title: Probing the binding of tetraplatinum(pyridyl)porphyrin complexes to DNA by means of surface plasmon resonance.
abstract: Tetrapyridylporphyrins containing four chloro(2,2'-bipyridine)platinum(II) complexes attached at the meta (3-H(2)TPtPyP) and para (4-H(2)TPtPyP) positions of the peripheral pyridine ligands were synthesized and their interaction with DNA investigated. The compounds were isolated in the solid state and characterized by means of spectroscopic and analytical techniques. According to molecular simulations, the two isomers exhibit contrasting structural characteristics, consistent with a saddle shape configuration for 3-H(2)TPtPyP and a planar geometry for 4-H(2)TPtPyP. Surface plasmon resonance studies were carried out on the interaction of the complexes with calf thymus DNA, revealing a preferential binding of 3-H(2)TPtPyP, presumably at the DNA major grooves.
authors.affiliation: Instituto de Química, Universidade de São Paulo, Caixa Postal 26077, CEP 05513-970 São Paulo, SP, Brazil.
authors.forename: Jeferson A
authors.initials: JA
authors.lastname: Naue
authors.forename: Sergio H
authors.initials: SH
authors.lastname: Toma
authors.forename: Juliano A
authors.initials: JA
authors.lastname: Bonacin
authors.forename: Koiti
authors.initials: K
authors.lastname: Araki
authors.forename: Henrique E
authors.initials: HE
authors.lastname: Toma
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D008665:Metalloporphyrins / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D017671:Platinum Compounds / Q000737:chemistry*; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D008024:Ligands; D008665:Metalloporphyrins; D009944:Organoplatinum Compounds; D017671:Platinum Compounds; D011166:Porphyrins; D004247:DNA