title: Association between polymorphisms of FCRL3, a non-HLA gene, and Behçet's disease in a Chinese population with ophthalmic manifestations.
abstract: OBJECTIVE
  Studies have shown a strong association of human leukocyte antigens-B51 (HLA-B51) with Behçet's disease (BD). However, little is known about the association of non-HLA genes with BD. The polymorphisms of the Fc receptor-like 3 gene (FCRL3), -169C/T, -110A/G, +358C/G, and +1381A/G, have been reported to be associated with several autoimmune diseases. This study was designed to determine whether the polymorphisms of FCRL3 were associated with susceptibility to BD in a Chinese population mainly with ocular involvement.
  
  
  METHODS
  A case-control study was performed in 245 Chinese BD patients and 289 controls. Four single nucleotide polymorphisms (SNPs; -169C/T, -110A/G, +358C/G, and +1381A/G) in FCRL3 were detected using polymerase chain reaction restriction fragment length polymorphisms (PCR-RFLP). HLA-B51 genotyping was performed by the PCR sequence specific primers method as described previously.
  
  
  RESULTS
  The results showed a significantly higher frequency of the G allele at the -110 site in BD patients compared with that in controls (corrected p=0.044, 75.3% versus 67.5%, chi2=7.72). Haplotype CGCG frequency was significantly higher in patients than in controls (corrected p=0.0096) whereas haplotype TACG frequency was significantly lower in patients compared with controls (corrected p=0.032). There was no relationship between clinical signs and FCRL3 polymorphisms. No significant difference was observed between patients and controls after HLA-B51 stratification concerning the four SNPs.
  
  
  CONCLUSIONS
  Our study suggests that the -110 G allele and the haplotype CGCG of FCRL3 are positively associated with BD in a Chinese population and that the haplotype ATCG might be a protective haplotype for BD.
authors.affiliation: First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Shengping
authors.initials: S
authors.lastname: Hou
authors.forename: Liping
authors.initials: L
authors.lastname: Du
authors.forename: Aize
authors.initials: A
authors.lastname: Kijlstra
authors.forename: Peizeng
authors.initials: P
authors.lastname: Yang
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D001528:Behcet Syndrome / Q000150:complications* / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control; D005128:Eye Diseases / Q000209:etiology*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015235:HLA-B Antigens / Q000097:blood / Q000235:genetics; D059949:HLA-B51 Antigen; D006239:Haplotypes; D006801:Humans; D011110:Polymorphism, Genetic; D011971:Receptors, Immunologic / Q000235:genetics*; D055815:Young Adult
chemical_list: C457775:FCRL3 protein, human; D015235:HLA-B Antigens; D059949:HLA-B51 Antigen; D011971:Receptors, Immunologic