Influence of extracellular matrix on cytokine stimulated pro-labour gene expression in human uterine myocytes.
Cellular function is modulated by the interaction with the extracellular matrix within the myometrium. We formed the hypothesis that the cytokine-stimulated pro-labour gene expression by human uterine smooth muscle cells would be increased by growing the cells on collagen-coated plates. Primary cultures of human uterine smooth muscle cells grown on uncoated plates and on plates coated with collagen were exposed to the inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta and interleukin-6) and assessed the messenger RNA expression of oxytocin receptor, interleukin-8, prostaglandin H synthase type-2 and prostaglandin F(2) alpha receptor. Basal pro-labour gene expression was unaffected by collagen coating and the response to the inflammatory cytokines was similar for oxytocin receptor and prostaglandin H synthase type-2, but appeared to be reduced for interleukin-8 and enhanced for FP. Collagen coating made no significant impact on basal integrin expression and interleukin-1beta induced phosphorylation of extracellular-regulated-kinase1/2 and RelA subunit of nuclear factor-kappa B (p65). We conclude that growing human uterine smooth muscle cells on collagen-coated plates may modulate the pro-labour gene response to the inflammatory cytokines.
Engineer
Neelam
N
Department of Maternal Fetal Medicine, Imperial College School of Medicine, Chelsea & Westminster Hospital, London, UK. nejengineer@aol.com
Sooranna
Suren R
SR
Liang
Zhiqing
Z
Bennett
Phillip R
PR
Johnson
Mark R
MR
D000328:Adult; D016207:Cytokines / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007743:Labor, Obstetric / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology; D009215:Myometrium / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D016207:Cytokines; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger