An enzyme cocktail for efficient protoplast formation in Aspergillus niger.
Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia.
de Bekker
Charissa
C
Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Wiebenga
Ad
A
Aguilar
Guillermo
G
Wösten
Han A B
HA
D000818:Animals; D001234:Aspergillus niger / Q000648:ultrastructure*; D002688:Chitinases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006372:Helix, Snails / Q000201:enzymology; D011523:Protoplasts; D013305:Streptomyces griseus / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology
D002688:Chitinases; D005966:Glucuronidase