Cytotoxicity and scanning electron microscopy study of gentamycin-coated HA effect on biofilm.
Biofilms are adherent, multi-layered colonies of bacteria that are typically more resistant to the host immune response and routine antibiotic therapy. HA biomaterial comprises of a single-phased hydroxyapatite scaffold with interconnected pore structure. The device is designed as osteoconductive space filler to be gently packed into bony voids or gaps following tooth extraction or any surgical procedure. Gentamycin-coated biomaterial (locally made hydroxyapatite) was evaluated to reduce or eradicate the biofilm on the implant materials. The results indicated that the HA coated with gentamycin was biocompatible to human osteoblast cell line and the biofilm has been reduced after being treated with different concentrations of gentamycin-coated hydroxyapatite (HA).
Au
L F
LF
Department of Human Anatomy, Industrial Technology Division, Malaysian Nuclear Agency.
Othman
F
F
Mustaffa
R
R
Vidyadaran
S
S
Rahmat
A
A
Besar
I
I
Akim
A Md
AM
Khan
M A K Goriman
MA
Saidi
M
M
Shamsudin
M Nor
MN
Froemming
G A
GA
Ishak
A K
AK
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials; D018441:Biofilms; D003602:Cytotoxicity, Immunologic; D017886:Durapatite; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods; D010006:Osteoblasts; D054457:Tissue Scaffolds
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005839:Gentamicins; D017886:Durapatite