A model of the complex between human beta-microseminoprotein and CRISP-3 based on NMR data.
beta-Microseminoprotein (MSP), a 10kDa seminal plasma protein, forms a tight complex with cysteine-rich secretory protein 3 (CRISP-3) from granulocytes. The 3D structure of human MSP has been determined but there is as yet no 3D structure for CRISP-3. We have now studied the complex between human MSP and CRISP-3 with multidimensional NMR. (15)N-HSQC spectra show substantial differences between free and complexed hMSP. Using several 3D-NMR spectra of triply labeled hMSP in complex with a recombinant N-terminal domain of CRISP-3, most of the backbone of hMSP could be assigned. The data show that only one side of hMSP, comprising beta-strands 1, 4, 5, and 8 are affected by the complex formation, indicating that beta-strands 1 and 8 form the main binding surface. Based on this we present a tentative structure for the hMSP-CRISP-3 complex using the known crystal structure of triflin as a model of CRISP-3.
Ghasriani
Houman
H
Department of Biophysical Chemistry, Lund University, Po Box 124, 22100 Lund, Sweden.
Fernlund
Per
P
Udby
Lene
L
Drakenberg
Torbjörn
T
D000595:Amino Acid Sequence; D003461:Crystallography; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D029584:Prostatic Secretory Proteins / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000378:metabolism; D029589:Seminal Plasma Proteins / Q000737:chemistry* / Q000378:metabolism
C489926:CRISP3 protein, human; D029584:Prostatic Secretory Proteins; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein