Surface-enhanced Raman scattering tags for rapid and homogeneous detection of circulating tumor cells in the presence of human whole blood.
A novel, homogeneous SERS-based cell detection assay was developed for rapid and direct enumeration of circulating tumor cells in the presence of whole blood. Magnetic beads and SERS tags were respectively conjugated to EpCAM and her2 antibodies for the capture and detection of approximately 50 tumor cells/mL in the presence of whole blood in less than 1 h.
Sha
Michael Y
MY
Oxonica Inc., 325 East Middlefield Road, Mountain View, California 94043, USA. mike_sha168@yahoo.com
Xu
Hongxia
H
Natan
Michael J
MJ
Cromer
Remy
R
D000951:Antigens, Neoplasm / Q000737:chemistry; D017209:Apoptosis; D001769:Blood / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D012542:Scattering, Radiation; D015398:Signal Transduction; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule