Comparison of Neisseria gonorrhoeae multiantigen sequence typing and porB sequence analysis for identification of clusters of N. gonorrhoeae isolates.
porB DNA sequence analysis and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) methods were compared for their abilities to discriminate strains and to identify epidemiologically congruent pairs of N. gonorrhoeae. Both methods provided high-level discrimination of strains. NG-MAST further differentiated large porB-based clusters. However, considerations of cost suggest that porB DNA sequence analysis is a useful tool for preliminary molecular analysis of the epidemiology of N. gonorrhoeae.
Liao
Mingmin
M
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Helgeson
Sarah
S
Gu
Wei-Ming
WM
Yang
Yang
Y
Jolly
Ann M
AM
Dillon
Jo-Anne R
JA
D000942:Antigens, Bacterial / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018272:Porins / Q000235:genetics*; D017421:Sequence Analysis
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D018272:Porins; C057699:porin protein, Neisseria