Interaction of the apolipoprotein E receptors low density lipoprotein receptor-related protein and sorLA/LR11.
In this study, we examined protein-protein interactions between two neuronal receptors, low density lipoprotein receptor-related protein (LRP) and sorLA/LR11, and found that these receptors interact, as indicated by three independent lines of evidence: co-immunoprecipitation experiments on mouse brain extracts and mouse neuronal cells, surface plasmon resonance analysis with purified human LRP and sorLA, and fluorescence lifetime imaging microscopy (FLIM) on rat primary cortical neurons. Immunocytochemistry experiments revealed widespread co-localization of LRP and sorLA within perinuclear compartments of rat primary neurons, while FLIM analysis showed that LRP-sorLA interactions take place within a subset of these compartments.
Spoelgen
R
R
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, MA 02129, USA.
Adams
K W
KW
Koker
M
M
Thomas
A V
AV
Andersen
O M
OM
Hallett
P J
PJ
Bercury
K K
KK
Joyner
D F
DF
Deng
M
M
Stoothoff
W H
WH
Strickland
D K
DK
Willnow
T E
TE
Hyman
B T
BT
D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D026502:LDL-Receptor Related Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods; D014162:Transfection / Q000379:methods
D026502:LDL-Receptor Related Proteins; D011973:Receptors, LDL; D049452:Green Fluorescent Proteins