[Effect of silver-hydroxyapatite/titania nanocomposites on proliferation, vitality and ultrastructure of osteoblasts].
OBJECTIVE
Osteoblasts behaviors were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg-HA/TiO2) in vitro.


METHODS
Rat calvaria osteoblasts were cultured in the presence of 10 mirog/mL-500 mirog/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope (TEM).


RESULTS
Both cell proliferation and vitality were restricted by addition of nanoparticles, while the respiration rates as an index for unit cell viability were enhanced. nAg-HA/TiO2 at low concentration showed superiority. No remarkable ultrastructural changes were observed in the osteoblasts exposed to nanoparticles. The differences in cell proliferation and cell vitality between nAg-HA/TiO2 group and nHA group at the same concentration were insignificant.


CONCLUSIONS
nAg-HA/TiO2 restricted cells proliferation, but triggered unit cell viability. Cytocompatibility of nAg-HA/TiO2 is almost the same as that of nHA's.
Zhang
Jing-chao
JC
Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China.
Mo
An-chun
AC
Wu
Hong-kun
HK
Liao
Juan
J
Li
Yu-bao
YB
Li
Xiao-yu
XY
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry; D004622:Embryo, Mammalian; D053761:Nanocomposites; D010006:Osteoblasts / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
C009495:titanium dioxide; D012834:Silver; D017886:Durapatite; D014025:Titanium