title: An integrated decision-tree testing strategy for eye irritation with respect to the requirements of the EU REACH legislation.
abstract: This paper presents some results of a joint research project, sponsored by Defra and conducted by FRAME and Liverpool John Moores University, on the status of alternatives to animal testing with regard to the European Union REACH (Registration, Evaluation and Authorisation of Chemicals) system for the safety testing and risk assessment of chemicals. The project covered all the main toxicity endpoints associated with REACH. This paper focuses on the use of alternative (non-animal) methods (both in vitro and in silico) for eye irritation testing. The manuscript reviews numerous in vitro tests and their possible collation into test batteries, in silico models and a refined in vivo method (the low volume eye test), before combining the use of all these methods into an integrated testing strategy. The aim of this strategy is a reduction in the number of animal tests which would need to be performed in the process of fulfilling the REACH system criteria; this would also lead to a lowering of the number of animals required in compliance with the REACH system requirements.
authors.affiliation: FRAME, Nottingham, UK. frame@frame.org.uk
authors.forename: Christina
authors.initials: C
authors.lastname: Grindon
authors.forename: Robert
authors.initials: R
authors.lastname: Combes
authors.forename: Mark T D
authors.initials: MT
authors.lastname: Cronin
authors.forename: David W
authors.initials: DW
authors.lastname: Roberts
authors.forename: John F
authors.initials: JF
authors.lastname: Garrod
mesh_terms: D000826:Animal Testing Alternatives / Q000331:legislation & jurisprudence*; D000818:Animals; D002478:Cells, Cultured; D003198:Computer Simulation; D003663:Decision Trees; D005062:European Union; D005128:Eye Diseases / Q000139:chemically induced*; D006801:Humans; D011817:Rabbits; D018675:Toxicity Tests / Q000379:methods*