Five year maintenance of the inducible expression of anthranilate synthase in Catharanthus roseus hairy roots.
Transgenic hairy root cultures have the potential to be an industrial production platform for a variety of chemicals. This report demonstrates the long-term stability of a transgenic Catharanthus roseus hairy root line containing the inducible expression of a feedback-insensitive anthranilate synthase (AS). After 5 years in liquid culture, the presence of the inserted AS gene was confirmed by genomic PCR. The inducible expression of AS was confirmed by enzyme assay and by changes in terpenoid indole alkaloid concentrations. This report also demonstrates that it may take as long as 2 years for the metabolite profile to stabilize.
Peebles
Christie A M
CA
Department of Bioengineering, Rice University, Houston, MS-3362, 6100 Main Street, Houston, Texas 77005, USA.
Sander
Guy W
GW
Li
Mai
M
Shanks
Jacqueline V
JV
San
Ka-Yiu
KY
D000878:Anthranilate Synthase / Q000096:biosynthesis*; D030008:Catharanthus / Q000201:enzymology*; D018744:DNA, Plant / Q000235:genetics; D042822:Genomic Instability; D030821:Plants, Genetically Modified / Q000201:enzymology; D016133:Polymerase Chain Reaction; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism
D018744:DNA, Plant; D046948:Secologanin Tryptamine Alkaloids; D000878:Anthranilate Synthase