Comparison of dentoalveolar morphology in WT and P2X7R KO mice for the development of biomechanical orthodontic models.
It has been suggested that the absence of the P2X7 receptor affects long bone morphology, and that one of the cytokines dependent on its activation may also affect tooth morphology. P2X7R KO (knockout) were compared with C57B/6 WT mice (background strain) to identify differences in a maxillary molar and surrounding bone. Nineteen WT and 12 KO mouse maxillae were scanned and 3D-reconstructed using microCT. Tooth dimensions were measured and 3D bone morphometry was conducted. A finite element model was constructed based on the results. No statistically significant differences were found in dentoalveolar characteristics between the two mouse types. A single finite element model of the tooth can be used to mechanically represent both strains. P2X7R does not have a major effect on alveolar bone or tooth morphology. The P2X7R effects are site-specific.
Viecilli
Rodrigo
R
Department of Orthodontics and Oral Facial Genetics, Indiana University School of Dentistry, 1121 W. Michigan St., Indianapolis, IN 46224, USA. rviecill@iupui.edu
Katona
Thomas
T
Chen
Jie
J
Roberts
Eugene
E
Hartsfield
James
J
D000539:Alveolar Process / Q000033:anatomy & histology* / Q000000981:diagnostic imaging / Q000502:physiology*; D000818:Animals; D001696:Biomechanical Phenomena; D001732:Bite Force; D020342:Finite Element Analysis; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D003765:Models, Dental; D009970:Orthodontics; D018048:Receptors, Purinergic P2 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D058486:Receptors, Purinergic P2X7; D014070:Tooth / Q000033:anatomy & histology* / Q000000981:diagnostic imaging / Q000502:physiology*; D055114:X-Ray Microtomography
C546469:P2rx7 protein, mouse; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7