Activity lysosomal enzymes in rat skin fibroblasts after treatment with progesterone and new gestagen ABMP.
The effects of a new synthetic gestagen 17alpha-acetoxy-3beta-butanoyloxy-6-methyl-pregna-4,6-dien-20-on (ABMP) and reference drug progesterone on rat skin fibroblasts were evaluated by variations in lysosomal enzyme activity (cathepsin D and beta-glucosidase). Our results suggest that ABMP exhibits lysosomotropic properties, which depended on its concentration and time of treatment. The direct effect of progesterone on lysosomal enzyme activity in skin fibroblasts was compared to the influence of systemic treatment with gestagens on skin lysosomes. The data indicate that local application of gestagen preparations holds much promise for the therapy of skin diseases accompanied by increased proliferation (e.g. psoriasis).
Liga
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AB
Department of Molecular Pharmacology and Radiobiology, Medical and Biological Faculty, Russian State Medical University, Moscow. ariuna.liga@gmail.com
Ukhina
T V
TV
Shimanovski
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NL
D019326:17-alpha-Hydroxyprogesterone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D011374:Progesterone / Q000494:pharmacology* / Q000627:therapeutic use; D011372:Progestins / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012871:Skin Diseases / Q000188:drug therapy / Q000473:pathology; D001617:beta-Glucosidase / Q000378:metabolism*
C498329:17alpha-acetoxy-3beta-butanoyloxy-6-methyl-pregna-4,6-dien-20-one; D011372:Progestins; D011374:Progesterone; D019326:17-alpha-Hydroxyprogesterone; D001617:beta-Glucosidase; D002402:Cathepsin D