[Establishing an experimental guinea pig model of dermatophytosis Using Trichophyton rubrum].
OBJECTIVE
To construct an animal model infected by Trichophyton rubrum.


METHODS
Three different strains of Trichophyton rubrum were separated from clinical specimen for the infection of guinea pigs. Corticosteroids were given before and after the construction of animal model to facilitate the infection. Direct microscopy, culture, and histopathologic methods were adopted to verify the construction.


RESULTS
Ten days after the inoculation of Trichophyton rubrum, with the intervention of corticosteroid, the guinea pigs were examined. Prominent scales and inflammation could be seen on the inoculation site of the Trichophyton rubrum infected guinea pig. Scales and hairs of Trichophyton rubrum infected guinea pig dealt with 10% potassium hydroxide, hypha out of the hair and microconidia or hypha in the hair shaft could be seen. Seven days after the inoculation of scales and hair on SDA plate, cultures of Trichophyton rubrum showed that the colonial morphology were identical to the original dermatophytes. PAS staining of infected guinea pig skin tissue showed that hypha and microconidia could be seen in the infundibula and hair root.


CONCLUSIONS
With the intervention of corticosteroid, a stable guinea pig model infected by Trichophyton rubrum were successfully constructed.
Chen
Xian-Jin
XJ
Department of Fungal Skin Disease, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
Shen
Yong-Nian
YN
Lü
Gui-Xia
GX
Liu
Wei-Da
WD
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D011897:Random Allocation; D014005:Tinea / Q000276:immunology / Q000382:microbiology*; D014249:Trichophyton / Q000472:pathogenicity / Q000502:physiology*