title: sodA is essential for virulence of Borrelia burgdorferi in the murine model of Lyme disease.
abstract: Borrelia burgdorferi, the causative agent of Lyme disease, has a limited set of genes to combat oxidative/nitrosative stress encountered in its tick vector or mammalian hosts. We inactivated the gene encoding for superoxide dismutase A (sodA, bb0153), an enzyme mediating the dismutation of superoxide anions and examined the in vitro and in vivo phenotype of the mutant. There were no significant differences in the in vitro growth characteristics of the sodA mutant compared with the control strains. Microscopic analysis of viability of spirochaetes revealed greater percentage of cell death upon treatment of sodA mutant with superoxide generators compared with its controls. Infectivity analysis in C3H/HeN mice following intradermal needle inoculation of 10(3) or 10(5) spirochaetes per mouse revealed complete attenuation of infectivity for the sodA mutant compared with control strains at 21 days post infection. The sodA mutant was more susceptible to the effects of activated macrophages and neutrophils, suggesting that its in vivo phenotype is partly due to the killing effects of activated immune cells. These studies indicate that SodA plays an important role in combating oxidative stress and is essential for the colonization and dissemination of B. burgdorferi in the murine model of Lyme disease.
authors.affiliation: South Texas Center for Emerging Infectious Diseases, and Department of Biology, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
authors.forename: Maria D
authors.initials: MD
authors.lastname: Esteve-Gassent
authors.forename: Nathaniel L
authors.initials: NL
authors.lastname: Elliott
authors.forename: J
authors.initials: J
authors.lastname: Seshu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D025065:Borrelia burgdorferi / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D005816:Genetic Complementation Test; D008193:Lyme Disease / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008809:Mice, Inbred C3H; D016296:Mutagenesis; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology; D018384:Oxidative Stress; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D013482:Superoxide Dismutase