Genetic mapping of Foxb1-cell lineage shows migration from caudal diencephalon to telencephalon and lateral hypothalamus.
The hypothalamus is a brain region with vital functions, and alterations in its development can cause human disease. However, we still do not have a complete description of how this complex structure is put together during embryonic and early postnatal stages. Radially oriented, outside-in migration of cells is prevalent in the developing hypothalamus. In spite of this, cell contingents from outside the hypothalamus as well as tangential hypothalamic migrations also have an important role. Here we study migrations in the hypothalamic primordium by genetically labeling the Foxb1 diencephalic lineage. Foxb1 is a transcription factor gene expressed in the neuroepithelium of the developing neural tube with a rostral expression boundary between caudal and rostral diencephalon, and therefore appropriate for marking migrations from caudal levels into the hypothalamus. We have found a large, longitudinally oriented migration stream apparently originating in the thalamic region and following an axonal bundle to end in the anterior portion of the lateral hypothalamic area. Additionally, we have mapped a specific expansion of the neuroepithelium into the rostral diencephalon. The expanded neuroepithelium generates abundant neurons for the medial hypothalamus at the tuberal level. Finally, we have uncovered novel diencephalon-to-telencephalon migrations into septum, piriform cortex and amygdala.
Zhao
Tianyu
T
Department of Genes and Behavior, Brain Development Group, Max Planck Institute of Biophysical Chemistry, Göttingen, Germany.
Szabó
Nora
N
Ma
Jun
J
Luo
Lingfei
L
Zhou
Xunlei
X
Alvarez-Bolado
Gonzalo
G
D000818:Animals; D001931:Brain Mapping / Q000379:methods; D019070:Cell Lineage / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D004027:Diencephalon / Q000196:embryology; D051858:Forkhead Transcription Factors / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D007026:Hypothalamic Area, Lateral / Q000196:embryology; D007031:Hypothalamus / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008967:Molecular Biology / Q000379:methods; D009434:Neural Pathways / Q000196:embryology; D054259:Neural Tube / Q000196:embryology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013687:Telencephalon / Q000196:embryology*
D051858:Forkhead Transcription Factors; C491332:Foxb1 protein, mouse; D005819:Genetic Markers