title: SUMOylation of RORalpha potentiates transcriptional activation function.
abstract: SUMOylation regulates a variety of cellular processes, including control of transcriptional activities of nuclear receptors. Here, we present SUMOylation of orphan nuclear receptor, RORalpha by both SUMO-1 and SUMO-2. SUMOylation of RORalpha occurred on the 240th lysine residue at the hinge region of human protein. PIAS family members, PIASxalpha, PIAS3, and PIASy, increased SUMOylation of RORalpha, whereas SENP2 specifically removed SUMO from RORalpha. SUMOylation-defective mutant form of RORalpha exhibited decreased transcriptional activity on RORalpha-responsive promoters indicating that SUMOylation may positively regulate transcriptional function of RORalpha.
authors.affiliation: Department of Biological Sciences, Research Center for Women's Disease, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742, South Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Hwang
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Jiyeong
authors.initials: J
authors.lastname: Jeong
authors.forename: Joo Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Young
authors.initials: Y
authors.lastname: Yang
authors.forename: Jong-Seok
authors.initials: JS
authors.lastname: Lim
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Baek
authors.forename: Keun Il
authors.initials: KI
authors.lastname: Kim
mesh_terms: D003546:Cysteine Endopeptidases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D011401:Promoter Regions, Genetic; D050828:Protein Inhibitors of Activated STAT / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D050828:Protein Inhibitors of Activated STAT; C539738:RORA protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C117909:SUMO2 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D015534:Trans-Activators; D003546:Cysteine Endopeptidases; C501833:SENP2 protein, human