A fluorescence sensor for detection of geranyl pyrophosphate by the chemo-ensemble method.
Hexaamide receptor 4 and coumarin phosphate 5 form an ensemble for effective sensing of geranyl pyrophosphate (GPP). Fluorescence resonance energy transfer in the 4 + 5 ensemble diminishes when 5 is replaced by GPP. Receptor 4 binds selectively with GPP over other anions, including fatty acids. The 4-GPP complex in 1:1 stoichiometry is inferred by NMR and fluorescence titrations. Receptor 4 contains a pseudotetrahedral cleft to accommodate GPP via multiple hydrogen bonds, and the two aliphatic chains exert additional hydrophobic interactions.
Chen
Kuan-Hung
KH
Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.
Liao
Jen-Hai
JH
Chan
Hsin-Yu
HY
Fang
Jim-Min
JM
D000577:Amides / Q000737:chemistry; D002138:Calibration; D003374:Coumarins / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011106:Polyisoprenyl Phosphates / Q000032:analysis*; D017186:Titrimetry
D000577:Amides; D003374:Coumarins; D005456:Fluorescent Dyes; D011106:Polyisoprenyl Phosphates; C015234:geranyl pyrophosphate; C030123:coumarin