Phosphorylation of nucleosides and nucleotides with inorganic monoimido-cyclo-triphosphate.
The phosphorylation of nucleosides (adenosine, guanosine, cytidine, and uridine) and nucleotides (adenosine 5'-monophosphate, guanosine 5'-monophosphate, cytidine 5'-monophosphate and uridine 5'-monophosphate) has been achieved using inorganic monoimido-cyclo-triphosphate (MCTP, Na(3)P(3)O(8)NH) in aqueous solution. In this reaction, the 2'-OH or 3'-OH group of the beta-D-ribofuranose unit was phosphorylated and the total yield was more than 30% and 14%, respectively. The main products were 2'-diphosphoramidophosphononucleoside and 2'-diphosphoramidophosphononucleoside 5'-monophosphate.
Maeda
Hideko
H
Department of Functional Molecular Chemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe, Japan.
Chiba
Takeshi
T
Tsuhako
Mitsutomo
M
Nakayama
Hirokazu
H
D003562:Cytidine / Q000737:chemistry; D006151:Guanosine / Q000737:chemistry; D007094:Imides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009705:Nucleosides / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D010766:Phosphorylation; D011122:Polyphosphates / Q000737:chemistry*; D014529:Uridine / Q000737:chemistry
D007094:Imides; D009705:Nucleosides; D009711:Nucleotides; D011122:Polyphosphates; C516188:monoimido-cyclo-triphosphate; D006151:Guanosine; D003562:Cytidine; D014529:Uridine