title: The bradykinin B2 receptor mediates hypoxia/reoxygenation induced neuronal cell apoptosis through the ERK1/2 pathway.
abstract: The bradykinin B2 receptor (B2R) mediates many physiological processes such as hypotension, inflammation and blood-vessel permeability. Hypoxia/reoxygenation (H/R) induces neuronal cell apoptosis. It was found that B2R expression was enhanced in primary cultured cortical neurons after H/R treatment. Addition of bradykinin (BK) alleviated the neuronal damage from H/R. This protective effect of BK was inhibited by the B2R antagonist, HOE140, and the ERK1/2 antagonist, PD98059. The phosphorylation of ERK1/2 was increased under H/R, and the addition of BK enhanced this effect. These results indicate that B2R plays an important role in protecting neurons from damage induced by H/R and this effect may function through the ERK1/2 pathway.
authors.affiliation: Department of neurology, Huashan Hospital, Fudan University, Shanghai 200040,China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Mei
authors.initials: M
authors.lastname: Cui
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Ren
authors.forename: Bao-Guo
authors.initials: BG
authors.lastname: Xiao
authors.forename: Ben-Yan
authors.initials: BY
authors.lastname: Luo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shao
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Hou-Guang
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D065094:Bradykinin B2 Receptor Antagonists; D015687:Cell Hypoxia; D002478:Cells, Cultured; D003470:Culture Media; D005419:Flavonoids / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043782:Receptor, Bradykinin B2 / Q000378:metabolism*; D015427:Reperfusion Injury / Q000503:physiopathology
chemical_list: D065094:Bradykinin B2 Receptor Antagonists; D003470:Culture Media; D005419:Flavonoids; D047428:Protein Kinase Inhibitors; D043782:Receptor, Bradykinin B2; C065679:icatibant; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D010100:Oxygen; D001920:Bradykinin; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one