title: Cytotoxicity and scanning electron microscopy study of gentamycin-coated HA effect on biofilm.
abstract: Biofilms are adherent, multi-layered colonies of bacteria that are typically more resistant to the host immune response and routine antibiotic therapy. HA biomaterial comprises of a single-phased hydroxyapatite scaffold with interconnected pore structure. The device is designed as osteoconductive space filler to be gently packed into bony voids or gaps following tooth extraction or any surgical procedure. Gentamycin-coated biomaterial (locally made hydroxyapatite) was evaluated to reduce or eradicate the biofilm on the implant materials. The results indicated that the HA coated with gentamycin was biocompatible to human osteoblast cell line and the biofilm has been reduced after being treated with different concentrations of gentamycin-coated hydroxyapatite (HA).
authors.affiliation: Department of Human Anatomy, Industrial Technology Division, Malaysian Nuclear Agency.
authors.forename: L F
authors.initials: LF
authors.lastname: Au
authors.forename: F
authors.initials: F
authors.lastname: Othman
authors.forename: R
authors.initials: R
authors.lastname: Mustaffa
authors.forename: S
authors.initials: S
authors.lastname: Vidyadaran
authors.forename: A
authors.initials: A
authors.lastname: Rahmat
authors.forename: I
authors.initials: I
authors.lastname: Besar
authors.forename: A Md
authors.initials: AM
authors.lastname: Akim
authors.forename: M A K Goriman
authors.initials: MA
authors.lastname: Khan
authors.forename: M
authors.initials: M
authors.lastname: Saidi
authors.forename: M Nor
authors.initials: MN
authors.lastname: Shamsudin
authors.forename: G A
authors.initials: GA
authors.lastname: Froemming
authors.forename: A K
authors.initials: AK
authors.lastname: Ishak
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials; D018441:Biofilms; D003602:Cytotoxicity, Immunologic; D017886:Durapatite; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods; D010006:Osteoblasts; D054457:Tissue Scaffolds
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005839:Gentamicins; D017886:Durapatite