title: Paramagnetic lipid-coated silica nanoparticles with a fluorescent quantum dot core: a new contrast agent platform for multimodality imaging.
abstract: Silica particles as a nanoparticulate carrier material for contrast agents have received considerable attention the past few years, since the material holds great promise for biomedical applications. A key feature for successful application of this material in vivo is biocompatibility, which may be significantly improved by appropriate surface modification. In this study, we report a novel strategy to coat silica particles with a dense monolayer of paramagnetic and PEGylated lipids. The silica nanoparticles carry a quantum dot in their center and are made target-specific by the conjugation of multiple alphavbeta3-integrin-specific RGD-peptides. We demonstrate their specific uptake by endothelial cells in vitro using fluorescence microscopy, quantitative fluorescence imaging, and magnetic resonance imaging. The lipid-coated silica particles introduced here represent a new platform for nanoparticulate multimodality contrast agents.
authors.affiliation: Condensed Matter and Interfaces, University Utrecht, P.O. Box 80000, 3508 TA Utrecht, The Netherlands.
authors.forename: Rolf
authors.initials: R
authors.lastname: Koole
authors.forename: Matti M
authors.initials: MM
authors.lastname: van Schooneveld
authors.forename: Jan
authors.initials: J
authors.lastname: Hilhorst
authors.forename: Karolien
authors.initials: K
authors.lastname: Castermans
authors.forename: David P
authors.initials: DP
authors.lastname: Cormode
authors.forename: Gustav J
authors.initials: GJ
authors.lastname: Strijkers
authors.forename: Celso
authors.initials: C
authors.lastname: de Mello Donegá
authors.forename: Daniel
authors.initials: D
authors.lastname: Vanmaekelbergh
authors.forename: Arjan W
authors.initials: AW
authors.lastname: Griffioen
authors.forename: Klaas
authors.initials: K
authors.lastname: Nicolay
authors.forename: Zahi A
authors.initials: ZA
authors.lastname: Fayad
authors.forename: Andries
authors.initials: A
authors.lastname: Meijerink
authors.forename: Willem J M
authors.initials: WJ
authors.lastname: Mulder
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003287:Contrast Media / Q000737:chemistry*; D005453:Fluorescence; D019786:Gadolinium DTPA / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D011092:Polyethylene Glycols / Q000737:chemistry; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D003287:Contrast Media; D008055:Lipids; C077628:gadolinium stearylamide DTPA; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D019786:Gadolinium DTPA