Derivation of cochlea hair cell for in vitro expansion and characterization.
A potential cure for hearing loss would be to regenerate hair cells by stimulating cells of the damaged inner ear sensory epithelia to proliferate and differentiate into hair cells. Here, we investigated the possibility to isolate, culture-expand and characterize the cells from the cochlea membrane of adult mice. Our results showed that the cultured cells isolated from mouse cochlea membrane were heterogenous in nature. Morphologically there were epithelial like cells, hair cell like, nerve cell like and fibroblastic cells observed in the culture. The cultured cells were immunopositive for specific hair cell markers including Myosin 7a, Calretinin and Espin.
Ibnubaidah
M A
MA
Department of Otorhinolaryngology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
Chua
K H
KH
Mazita
A
A
Azida
Z N
ZN
Aminuddin
B S
BS
Ruszymah
B H I
BH
Lokman
B S
BS
D000818:Animals; D015415:Biomarkers; D064032:Calbindin 2; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology*; D004848:Epithelium; D005347:Fibroblasts / Q000166:cytology; D006198:Hair Cells, Auditory / Q000166:cytology*; D051379:Mice; D008840:Microfilament Proteins; D009218:Myosins; D064030:S100 Calcium Binding Protein G
D015415:Biomarkers; C576511:Calb2 protein, mouse; D064032:Calbindin 2; D008840:Microfilament Proteins; D064030:S100 Calcium Binding Protein G; C104108:espin protein, mouse; D009218:Myosins