Production of purple-colored creeping bentgrass using maize transcription factor genes Pl and Lc through Agrobacterium-mediated transformation.
Purple-colored transgenic creeping bentgrass (Agrostis stolonifera L.) plants were developed for ornamental purpose by means of Agrobacterium-mediated transformation. Embryogenic creeping bentgrass calli were transformed with the pCAMBIA 3301 vector harboring maize (Zea mays) flavonoid/anthocyanin biosynthetic pathway transcription factor genes, Lc (Leaf color) and Pl (Purple leaf), individually and in combination, and three types of putative transgenic plants (Lc, Pl, and Lc + Pl) were generated. Genomic integration and expression of the transgenes were confirmed by Southern and northern blot analyses, respectively. The transgenic creeping bentgrass plants expressing both Lc and Pl genes were entirely purple, whereas those expressing Pl alone had purple stems and those expressing Lc alone lacked purple pigmentation in adult plants. The anthocyanin content was estimated in all the three types of transgenic plant and correlated well with the degree of purple coloration observed. These results suggest that both Lc and Pl genes are necessary and sufficient to confer purple coloration to creeping bentgrass.
Han
Yun-Jeong
YJ
Department of Biotechnology, Chonnam National University, Gwangju, South Korea.
Kim
Yong-Min
YM
Lee
Jee-Yeon
JY
Kim
Soo Jung
SJ
Cho
Kyu-Chang
KC
Chandrasekhar
Thummala
T
Song
Pill-Soon
PS
Woo
Young-Min
YM
Kim
Jeong-Il
JI
D031703:Agrostis / Q000235:genetics*; D000872:Anthocyanins / Q000096:biosynthesis; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D017343:Genes, Plant; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics*
D000872:Anthocyanins; D018744:DNA, Plant; D010940:Plant Proteins; D014157:Transcription Factors