[Optimization of signaling pathways for bone regeneration].
We have developed an ES cell-based monitoring system for osteogenic differentiation and identified a potent combination of osteogenic genes. ES cells were isolated from mice carrying a transgene expressing GFP driven by the 2.3 kb fragment of rat type I collagen alpha1 promoter. We have screened cDNA libraries and combinations of major osteogenesis-related genes and identified that the combination of constitutively active activin receptor-like kinase 6 (caALK6) and runt-related transcription factor 2 (Runx2) was the minimal unit that induced GFP. These combinations of genes would be a potent and ideal tool for bone regeneration.
Ogata
Naoshi
N
The University of Tokyo, Faculty of Medicine, Division of Bone and Cartilage Regeneration, Japan.
Chung
Ung-il
UI
Tei
Yuichi
Y
Ohba
Shinsuke
S
Kawaguchi
Hiroshi
H
D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I; D019485:Bone Morphogenetic Proteins; D001861:Bone Regeneration / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit; D053595:Embryonic Stem Cells; D049452:Green Fluorescent Proteins; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D044968:Regenerative Medicine / Q000379:methods; D015398:Signal Transduction / Q000235:genetics; D023822:Tissue Engineering / Q000379:methods
D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit; D049452:Green Fluorescent Proteins; C498215:Bmpr1b protein, mouse; D052005:Bone Morphogenetic Protein Receptors, Type I