Centella asiatica extract selectively decreases amyloid beta levels in hippocampus of Alzheimer's disease animal model.
PSAPP mice expressing the 'Swedish' amyloid precursor protein and the M146L presenilin 1 mutations are a well-characterized model for spontaneous amyloid beta plaque formation. Centella asiatica has a long history of use in India as a memory enhancing drug in Ayurvedic literature. The study investigated whether Centella asiatica extract (CaE) can alter the amyloid pathology in PSAPP mice by administering CaE (2.5 or 5.0 g/kg/day) starting at 2 months of age prior to the onset of detectable amyloid deposition and continued for either 2 months or 8 months. A significant decrease in amyloid beta 1-40 and 1-42 was detectable by ELISA following an 8 month treatment with 2.5 mg/kg of CaE. A reduction in Congo Red stained fibrillar amyloid plaques was detected with the 5.0 mg/kg CaE dose and long-term treatment regimen. It was also confirmed that CaE functions as an antioxidant in vitro, scavenging free radicals, reducing lipid peroxidation and protecting against DNA damage. The data indicate that CaE can impact the amyloid cascade altering amyloid beta pathology in the brains of PSAPP mice and modulating components of the oxidative stress response that has been implicated in the neurodegenerative changes that occur with Alzheimer's disease.
Dhanasekaran
Muralikrishnan
M
Department of Neurology, Scott and White Clinic, Texas A & M University System HSC College of Medicine, Texas, USA.
Holcomb
Leigh A
LA
Hitt
Angie R
AR
Tharakan
Binu
B
Porter
Jami W
JW
Young
Keith A
KA
Manyam
Bala V
BV
D000544:Alzheimer Disease / Q000188:drug therapy* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D000818:Animals; D028041:Centella / Q000737:chemistry*; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation; D008515:Medicine, Ayurvedic; D051379:Mice; D008822:Mice, Transgenic; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress; D010936:Plant Extracts; D058225:Plaque, Amyloid / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*
D016229:Amyloid beta-Peptides; C526146:Centella asiatica extract; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014315:Triterpenes