title: Transcription initiation in the Escherichia coli K-12 malI-malX intergenic region and the role of the cyclic AMP receptor protein.
abstract: The Escherichia coli K-12 malI-malX intergenic region contains two divergent promoters, which have been investigated by both mutational and biochemical analysis. The malX promoter drives transcription initiation from a location that is 43 bp upstream from the malX translation start codon. Expression from the malX promoter is dependent on binding of the cyclic AMP receptor protein (CRP) to a DNA site centred 41.5 bp upstream of the transcript start. The malI promoter drives transcription initiation from a location 85 bp upstream from the malX transcript start and it is active without the CRP. Expression from the malI promoter can be stimulated by the CRP. Mutational analysis suggests that the malI promoter has an unusual organization.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK.
authors.forename: Georgina S
authors.initials: GS
authors.lastname: Lloyd
authors.forename: Kerry
authors.initials: K
authors.lastname: Hollands
authors.forename: Rita E
authors.initials: RE
authors.lastname: Godfrey
authors.forename: Stephen J W
authors.initials: SJ
authors.lastname: Busby
mesh_terms: D001483:Base Sequence; D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D021901:DNA, Intergenic / Q000378:metabolism*; D048168:Escherichia coli K12 / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002373:Cyclic AMP Receptor Protein; D021901:DNA, Intergenic; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C481662:crp protein, E coli; C060788:MalI protein, E coli