Genetic diversity among Bolivian arenaviruses.
Machupo virus and Chapare virus are members of the Tacaribe serocomplex (virus family Arenaviridae) and etiological agents of hemorrhagic fever in humans in Bolivia. The nucleotide sequences of the complete Z genes, a large fragment of the RNA-dependent RNA polymerase genes, the complete glycoprotein precursor genes, and the complete nucleocapsid protein genes of 8 strains of Machupo virus were determined to increase our knowledge of the genetic diversity among the Bolivian arenaviruses. The results of analyses of the predicted amino acid sequences of the glycoproteins of the Machupo virus strains and Chapare virus strain 200001071 indicated that immune plasma from hemorrhagic fever cases caused by Machupo virus may prove beneficial in the treatment of Bolivian hemorrhagic fever but not hemorrhagic fever caused by Chapare virus.
Cajimat
Maria N B
MN
Microbiology and Immunology Graduate Program, Graduate School of Biomedical Sciences, The University of Texas Medical Branch, Galveston, TX 77555-1019, USA. nbcajirm@utmb.edu
Milazzo
Mary Louise
ML
Rollin
Pierre E
PE
Nichol
Stuart T
ST
Bowen
Michael D
MD
Ksiazek
Thomas G
TG
Fulhorst
Charles F
CF
D000595:Amino Acid Sequence; D000818:Animals; D001117:Arenaviridae Infections / Q000821:virology; D006477:Arenaviruses, New World / Q000145:classification / Q000235:genetics*; D001838:Bolivia; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics; D006478:Hemorrhagic Fever, American / Q000821:virology; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics
D006023:Glycoproteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D012324:RNA-Dependent RNA Polymerase