[Experimental study on culture of human mesenchymal stem cells from cord blood using autologous serum].
In this study, we collected human umbilical cord blood and separated the autologous serum and clear cells. Then the human mesenchymal stem cells were cultured with the use of autologous serum and fetal serum, respectively. By identifing surface markers with flow cytometry and testing the osteogenic, adipogenic and cardiomyocytes differentiation capacity of the cells, we affirmed that the cultured cells are mesenchymal stem cells. Among 21 cases of umbilical cord blood, 11 cases using fetal bovine serum and 15 cases using autologous successfully cultured mesenchymal stem cells from umbilical cord blood. The UCB-MSCs did not express CD34 and CD45, but they expressed CD29, CD44 and CD105, and they were successfully induced into osteogenic cells, adipogenic cells and cardiomyocytic-like cells. All of these suggest that using autologous serum to culture UCB-MSCs is better than using fetal bovine serum, and the autologous serum cultured UCB-MSCs can also be induced into osteogenic cells, adipogenic cells cardiomycyote-like cells.
Zhang
Yonggang
Y
Laboratory of Cell-biology, College of Life Science, Sichuan University, Chengdu 610064, China.
Wang
Liming
L
Yang
Mingli
M
Huang
Lin
L
Hu
Huozhen
H
D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D044967:Serum
D003470:Culture Media