Limiting dilution analysis of antigen-specific T cells.
Limiting dilution analysis (LDA) has been extensively employed as a quantitative method to estimate the precursor frequency of various T lymphocyte subsets according to their functional properties in vitro. We describe here an example of LDA experiment assessing antigen-specific T cell proliferation of microcultures in the presence or absence of adjuvant and illustrate how to estimate the frequencies of precursor T cells using an online tool that we made publicly available.
Carneiro
Jorge
J
Instituto Gulbenkian de Ciência, Oeiras, Portugal.
Duarte
Lurdes
L
Padovan
Elisabetta
E
D000941:Antigens / Q000276:immunology*; D049109:Cell Proliferation / Q000528:radiation effects; D045142:Cross-Priming / Q000276:immunology / Q000528:radiation effects; D016207:Cytokines / Q000378:metabolism; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D013234:Stem Cells / Q000166:cytology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000528:radiation effects
D000941:Antigens; D016207:Cytokines; D000939:Epitopes