title: Structure of a RSC-nucleosome complex and insights into chromatin remodeling.
abstract: ATP-dependent chromatin-remodeling complexes, such as RSC, can reposition, evict or restructure nucleosomes. A structure of a RSC-nucleosome complex with a nucleosome determined by cryo-EM shows the nucleosome bound in a central RSC cavity. Extensive interaction of RSC with histones and DNA seems to destabilize the nucleosome and lead to an overall ATP-independent rearrangement of its structure. Nucleosomal DNA appears disordered and largely free to bulge out into solution as required for remodeling, but the structure of the RSC-nucleosome complex indicates that RSC is unlikely to displace the octamer from the nucleosome to which it is bound. Consideration of the RSC-nucleosome structure and published biochemical information suggests that ATP-dependent DNA translocation by RSC may result in the eviction of histone octamers from adjacent nucleosomes.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Chaban
authors.forename: Chukwudi
authors.initials: C
authors.lastname: Ezeokonkwo
authors.forename: Wen-Hsiang
authors.initials: WH
authors.lastname: Chung
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kornberg
authors.forename: Barbara
authors.initials: B
authors.lastname: Maier-Davis
authors.forename: Yahli
authors.initials: Y
authors.lastname: Lorch
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Asturias
mesh_terms: D042002:Chromatin Assembly and Disassembly; D020285:Cryoelectron Microscopy; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008958:Models, Molecular; D009707:Nucleosomes / Q000737:chemistry* / Q000648:ultrastructure*; D020836:Protein Structure, Quaternary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D014157:Transcription Factors / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D004268:DNA-Binding Proteins; D009707:Nucleosomes; C103337:RSC complex, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors