title: [Screening and identification of proteins interacting with RAR alpha-V via yeast two-hybrid system].
abstract: OBJECTIVE
  To screen the protein interacting with retinoic acid receptor variant protein (RAR alpha-V) via the yeast two-hybrid technique (YTHT) and to find out the targets protein and study its biological function.
  
  
  METHODS
  The bait vector of pGBKT7-RAR alpha-V was constructed for screening proteins interacting with RAR alpha-V in K562 cell cDNA expression library via YTHT. The protein-protein interaction was confirmed with re-transformation in yeast and GST pull-down in vitro.
  
  
  RESULTS
  The bait vector was successfully constructed without toxicity, leakage and self-activation. Sixteen proteins were screened by YTHT and eight positive clones were identified by re-transformation in yeast. The interaction between RAR alpha-V and JTV-1 was confirmed by GST pull-down in vitro.
  
  
  CONCLUSIONS
  There are some kinds of proteins interacting with RAR alpha-V in cell. The biological dysfunction caused by certain protein-protein interaction may be involved in the pathogenesis of leukemia.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Bei-zhong
authors.initials: BZ
authors.lastname: Liu
authors.forename: Qian-feng
authors.initials: QF
authors.lastname: Xia
authors.forename: Po
authors.initials: P
authors.lastname: Hao
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Dan-ting
authors.initials: DT
authors.lastname: Jin
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
mesh_terms: D015723:Gene Library; D006801:Humans; D020014:K562 Cells; D025941:Protein Interaction Mapping; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D000072482:Retinoic Acid Receptor alpha; D020798:Two-Hybrid System Techniques
chemical_list: C000607071:RARA protein, human; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha