title: Variability, heritability and environmental determinants of human plasma N-glycome.
abstract: Plasma glycans were analyzed in 1008 individuals to evaluate variability and heritability, as well as the main environmental determinants that affect glycan structures. By combining HPLC analysis of fluorescently labeled glycans with sialidase digestion, glycans were separated into 33 chromatographic peaks and quantified. A high level of variability was observed with the median ratio of minimal to maximal values of 6.17 and significant age- and gender-specific differences. Heritability estimates for individual glycans varied widely, ranging from very low to very high. Glycome-wide environmental determinants were also detected with statistically significant effects of different variables including diet, smoking and cholesterol levels.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Zagreb, Zagreb, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Knezević
authors.forename: Ozren
authors.initials: O
authors.lastname: Polasek
authors.forename: Olga
authors.initials: O
authors.lastname: Gornik
authors.forename: Igor
authors.initials: I
authors.lastname: Rudan
authors.forename: Harry
authors.initials: H
authors.lastname: Campbell
authors.forename: Caroline
authors.initials: C
authors.lastname: Hayward
authors.forename: Alan
authors.initials: A
authors.lastname: Wright
authors.forename: Ivana
authors.initials: I
authors.lastname: Kolcic
authors.forename: Niaobh
authors.initials: N
authors.lastname: O'Donoghue
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bones
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004777:Environment; D005260:Female; D014644:Genetic Variation; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D011134:Polysaccharides / Q000097:blood / Q000737:chemistry; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides