Glucose sensing electrodes based on a poly(3,4-ethylenedioxythiophene)/Prussian blue bilayer and multi-walled carbon nanotubes.
Here we report a new glucose sensing electrode based on a poly(3,4-ethylenedioxythiophene) (PEDOT)/Prussian blue (PB) bilayer and multi-walled carbon nanotubes (CNT). The bilayer was prepared on a flexible screen-printed carbon electrode (SPCE) by sequential electrodeposition. The inner PB layer was electrodeposited first for detecting H(2)O(2) from glucose oxidation; the outer PEDOT layer was electropolymerized on a baked or an unbaked PB film to entrap glucose oxidase (GOD). It was observed that the stability of PB in phosphate buffered saline (pH 7.4) was attained by post-deposition bake at 100 degrees C and the outer PEDOT layer both. In addition, a baked PB film enhanced the subsequent PEDOT growth and the corresponding GOD entrapment. As a result, the bilayer enzyme electrode showed highly resolved and reproducible signals (R.S.D.=2.54%) to glucose samples from 100 microM to 1M during a flow-injection analysis (FIA) at -0.1V vs. Ag/AgCl. The sensitivity of the linear range (1-10mM) was 2.67 microAcm(-2)mM(-1). Moreover, the electrode retained ca. 82% of the original response after 1-month storage in PBS, pH 6.0 at 4 degrees C and could determine the glucose level in human serum precisely. Besides, it was found that CNT incorporation could further improve the sensitivity and could achieve muM-range glucose detection.
Chiu
Jing-Yang
JY
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan.
Yu
Chung-Mu
CM
Yen
Miao-Ju
MJ
Chen
Lin-Chi
LC
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005295:Ferrocyanides / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D008839:Microelectrodes; D036103:Nanotechnology / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D019086:Bridged Bicyclo Compounds, Heterocyclic; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D037742:Nanotubes, Carbon; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide