A new bio-active surface for protein immobilisation via copper-free 'click' between azido SAM and alkynyl Fischer carbene complex.
Fischer carbene complex anchored on glass or silicon surface using a Cu-free 'click' reaction allows facile and swift covalent grafting of protein molecules like Bovine Serum Albumin (BSA).
Sawoo
Sudeshna
S
Department of Organic Chemistry, Indian Association for the Cultivation of Science, Kolkata-700032, India.
Dutta
Piyali
P
Chakraborty
Amarnath
A
Mukhopadhyay
Rupa
R
Bouloussa
Othman
O
Sarkar
Amitabha
A
D000818:Animals; D001386:Azides / Q000138:chemical synthesis* / Q000737:chemistry; D002417:Cattle; D003619:Dansyl Compounds / Q000138:chemical synthesis / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017640:Silicates / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D001386:Azides; D003619:Dansyl Compounds; D055767:Immobilized Proteins; D017640:Silicates; C030011:carbene; D012710:Serum Albumin, Bovine; D008697:Methane; C029038:dansyl chloride