title: Application of the glucose hydrogen breath test for the detection of bacterial overgrowth in patients with cystic fibrosis--a reliable method?
abstract: Patients with cystic fibrosis (CF) have recently been deemed highly susceptible for bacterial intestinal overgrowth (BIO). We aimed to define the prevalence of BIO in children with CF by applying the H(2)-glucose breath test. Forty children with CF and ten healthy children received 1 g/kg D-glucose orally. Breath samples for H(2) content (ppm) were collected for 3 h. BIO was suspected if the breath hydrogen content increased by more than 20 ppm or if baseline concentrations topped 20 ppm. In 27 of 40 CF children (68%), breath hydrogen content exceeded 20 ppm. Whereas the breath hydrogen exhalation persisted above 20 ppm in almost all these children throughout the sampling period, none of the remaining children increased above this threshold. The high rate of CF children with elevated fasting hydrogen breath concentrations indicates that this phenomenon is less a sign of BIO rather than a consequence of global malabsorption and intestinal dysmotility.
authors.affiliation: Medical Department I (ZAFES), Johann Wolfgang Goethe-University Frankfurt, Theodor-Stern-Kai 7, Frankfurt/Main, 60590, Germany. arne.schneider@em.uni-frankfurt.de
authors.forename: Arne R J
authors.initials: AR
authors.lastname: Schneider
authors.forename: Stefanie
authors.initials: S
authors.lastname: Klueber
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Posselt
authors.forename: Benjamin
authors.initials: B
authors.lastname: Funk
authors.forename: Lydia
authors.initials: L
authors.lastname: Murzynski
authors.forename: Wolfgang F
authors.initials: WF
authors.lastname: Caspary
authors.forename: Juergen
authors.initials: J
authors.lastname: Stein
mesh_terms: D000293:Adolescent; D001944:Breath Tests / Q000379:methods*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000378:metabolism*; D005260:Female; D005769:Gastrointestinal Motility / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology; D007422:Intestines / Q000382:microbiology* / Q000503:physiopathology; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006859:Hydrogen; D005947:Glucose