title: Cell based therapy for osteoarthritis in a sheep model: gross and histological assessment.
abstract: This study was to determine if autologous bone marrow mesenchymal stem cells (BMSCs) cultured in chondrogenic medium could repair surgically induced osteoarthritis. Sheep BMSCs were cultured in medium containing 5ng/ml TGFbeta3 + 50ng/ml IGF-1 for three weeks. The cultured cells were then suspended at density of 2x10(6) cell/ml and injected intraarticularly into the osteoarthritic knee joint. After six weeks, the distal head of the femur and the proximal tibial plateau were removed and stained with H&E. The results indicated that knee joints treated with autologous BMSCs cultured in chondrogenic medium showed clear evidence of articular cartilage regeneration in comparison with other groups.
authors.affiliation: Department of Physiology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
authors.forename: H
authors.initials: H
authors.lastname: Alfaqeh
authors.forename: M Y
authors.initials: MY
authors.lastname: Norhamdan
authors.forename: K H
authors.initials: KH
authors.lastname: Chua
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
authors.forename: B S
authors.initials: BS
authors.lastname: Aminuddin
authors.forename: B H I
authors.initials: BH
authors.lastname: Ruszymah
mesh_terms: D000818:Animals; D001853:Bone Marrow; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D005269:Femur / Q000473:pathology; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D010003:Osteoarthritis / Q000473:pathology / Q000628:therapy*; D044968:Regenerative Medicine; D034561:Sheep, Domestic; D013977:Tibia / Q000473:pathology; D014182:Transplantation, Autologous