title: Molecular cloning of Kazal-type proteinase inhibitor of the shrimp Fenneropenaeus chinensis.
abstract: Proteinase inhibitors play important roles in host defence systems involving blood coagulation and pathogen digestion. We isolated and characterized a cDNA clone for a Kazal-type proteinase inhibitor (KPI) from a hemocyte cDNA library of the oriental white shrimp Fenneropenaeus chinensis. The KPI gene consists of three exons and two introns. KPI cDNA contains an open reading frame of 396 bp, a polyadenylation signal sequence AATAAA, and a poly (A) tail. KPI cDNA encodes a polypeptide of 131 amino acids with a putative signal peptide of 21 amino acids. The deduced amino acid sequence of KPI contains two homologous Kazal domains, each with six conserved cysteine residues. The mRNA of KPI is expressed in the hemocytes of healthy shrimp, and the higher expression of KPI transcript is observed in shrimp infected with the white spot syndrome virus (WSSV), suggesting a potential role for KPI in host defence mechanisms.
authors.affiliation: Biotechnology Research Institute, National Fisheries Research and Development Institute, Gijang-gun, Busan, South Korea. hjkong@nfrdi.re.kr
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kong
authors.forename: Hyun Kook
authors.initials: HK
authors.lastname: Cho
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Gyeong-Eun
authors.initials: GE
authors.lastname: Hong
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hyon Sob
authors.initials: HS
authors.lastname: Han
authors.forename: In-Kwon
authors.initials: IK
authors.lastname: Jang
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Lee
authors.forename: Jaehun
authors.initials: J
authors.lastname: Cheong
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D015842:Serine Proteinase Inhibitors