The effects of hydroxyapatite/calcium phosphate glass scaffold and its surface modification with bovine serum albumin on 1-wall intrabony defects of beagle dogs: a preliminary study.
The purpose of this study was to evaluate the effects of biphasic hydroxyapatite/calcium phosphate glass (HA/CPG) scaffold and its surface modification with bovine serum albumin (BSA) on periodontal regeneration. 1-wall intrabony defects were surgically created on five beagle dogs. HA/CPG scaffolds, with a hydroxyapatite (HA)/calcium phosphate glass (CPG) ratio of 95:5 by weight (%) and surface modification done by 2% bovine serum albumin, were used. The control group received surgical flap operation, and the experimental groups were filled with HA/CPG scaffolds and HA/CPG(BSA) scaffolds. The animals were sacrificed eight weeks after surgery. Histological findings revealed better space maintenance in the experimental groups than the control group, and showed new bone formation intermittently in between the residual material particles. The newly formed bone was mostly woven bone and the residual particles were undergoing resorption. Cementum regeneration was observed with limited root resorption in all the groups. Histometric analysis also revealed greater mean values in new bone formation, cementum regeneration and bone area than the control group in both experimental groups. However, similar findings were presented between HA/CPG and HA/CPG(BSA). The result of the present study revealed the newly fabricated HA/CPG scaffold to have a potential use as a bone substitute material.
Um
Yoo-Jung
YJ
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, 134 Shinchon-Dong, Seodaemun-gu, Seoul 120-752 l, Korea.
Jung
Ui-Won
UW
Chae
Gyung-Joon
GJ
Kim
Chang-Sung
CS
Lee
Yong-Keun
YK
Cho
Kyoo-Sung
KS
Kim
Chong-Kwan
CK
Choi
Seong-Ho
SH
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D001862:Bone Resorption; D018786:Bone Substitutes / Q000737:chemistry; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D002417:Cattle; D003739:Dental Cementum / Q000737:chemistry; D004285:Dogs; D017886:Durapatite / Q000737:chemistry*; D005898:Glass; D008422:Materials Testing; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes; D002130:Calcium Phosphates; D012710:Serum Albumin, Bovine; D017886:Durapatite; C020243:calcium phosphate