Proton pump OsA8 is linked to phosphorus uptake and translocation in rice.
The plasma membrane (PM) proton pump ATPases (H(+)-ATPases) are involved in almost all aspects of biology. They are plant specific and several members of this family are supposed to play a key role in nutrient acquisition. At present, only some members of this gene family in plants have been characterized. However, no nutrient uptake associated H(+)-ATPase gene in rice has been functionally analysed. It is reported here that OsA8, a typical PM H(+)-ATPases gene that was predominantly expressed in roots of rice, is down-regulated by nutrient deficiency. The Osa8 mutant had a relatively smaller size and root to shoot biomass ratio, but higher ATPase activity than its wild-type counterparts under phosphorus (P) starvation conditions. Knockout of OsA8 affected the expression of several OsA genes and the high affinity phosphate transporter, OsPT6, and resulted in a higher P concentration in the roots and a lower amount of P in the shoots. These analyses demonstrate that OsA8 not only influences the uptake of P by roots, but also the translocation of P from the roots to the shoots in rice.
Chang
Chunrong
C
State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Hu
Yibing
Y
Sun
Shubin
S
Zhu
Yiyong
Y
Ma
Guojie
G
Xu
Guohua
G
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001692:Biological Transport; D018533:Biomass; D015536:Down-Regulation; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D006720:Homozygote; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics; D018520:Plant Shoots / Q000033:anatomy & histology / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D017494:Proton Pumps; D012333:RNA, Messenger; D010758:Phosphorus; D000251:Adenosine Triphosphatases