title: [Increased expression of KCTD9, a novel potassium channel related gene, correlates with disease severity in patients with viral hepatitis B].
abstract: OBJECTIVE
  Studies have shown that potassium channel plays a pivotal role in T cell activation. The expression of potassium channel gene KCTD9 was evidenced being highly upregulated in patients with severe hepatitis B (SHB). To understand this phenomenon further, tissue and cellular expression profiles of KCTD9 were investigated in patients with SHB.
  
  
  METHODS
  A rabbit peptide polyclonal antibody was prepared. Various samples including peripheral blood mononuclear cells (PBMCs); livers from patients with SHB or mild chronic hepatitis B, were examined for KCTD9 expression by quantitative real time PCR and immunohistochemistry staining (IHC). Confocal microscopy was used to illustrate the localizations of the expressions.
  
  
  RESULTS
  Increased expression of KCTD9 was observed in PBMC in over 35.7% of the patients with SHB when compared with that of patients with mild chronic hepatitis B. In all patients, the relative value of increased KCTD9 mRNA was positively correlated with alanine aminotransferase, aspartate aminotransferase, total bilirubin and direct bilirubin but negatively with serum albumin. The expression was mainly located in hepatocytes, bile duct epithelial cells, Kupffer cells and inflammatory cells, and in the cytoplasm of PBMCs from the healthy individuals and patients with mild chronic hepatitis B, whereas in both cytoplasm and nuclei in those from patients with SHB.
  
  
  CONCLUSIONS
  The increased expression of potassium channel gene KCTD9 correlates with disease severity in patients with viral hepatitis B.
authors.affiliation: Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yao-yong
authors.initials: YY
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Pi
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Guo-hong
authors.initials: GH
authors.lastname: Deng
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Hong-wu
authors.initials: HW
authors.lastname: Wang
authors.forename: Mei-fang
authors.initials: MF
authors.lastname: Han
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Yan
authors.forename: Dong
authors.initials: D
authors.lastname: Xi
authors.forename: Jia-quan
authors.initials: JQ
authors.lastname: Huang
authors.forename: Yu-ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Luo
authors.forename: Qin
authors.initials: Q
authors.lastname: Ning
mesh_terms: D000328:Adult; D005260:Female; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000821:virology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
chemical_list: C580589:KCTD9 protein, human; D015221:Potassium Channels; D012333:RNA, Messenger