title: Unprecedented biological cyclopropanation in the biosynthesis of FR-900848.
abstract: We were able to show the predominant incorporation of a single enantiomer and intact incorporation of multiply labelled synthetic diketide precursors (and), which established the intermediacy of cyclopropanated diketide and led to our proposal for the unprecedented biological cyclopropation, via PKS (polyketide synthase) having a novel cyclopropanase domain, in the biosynthesis of FR-900848 (1).
authors.affiliation: Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, 010-0195, Japan. tkwn@akita-pu.ac.jp
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Tokiwano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Seo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Oikawa
mesh_terms: D039903:Actinobacteria / Q000201:enzymology; D000214:Acyl Coenzyme A / Q000378:metabolism; D003521:Cyclopropanes / Q000494:pharmacology; D008954:Models, Biological; D009705:Nucleosides / Q000096:biosynthesis / Q000494:pharmacology; D048630:Polyketide Synthases / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000214:Acyl Coenzyme A; D003521:Cyclopropanes; D009705:Nucleosides; C065437:FR 900848; D048630:Polyketide Synthases