Novel quasi-interpenetrating network/functionalized multi-walled carbon nanotubes double-network composite matrices for DNA sequencing by CE.
Poly(N, N-dimethylacrylamide) (PDMA)-functionalized multi-walled carbon nanotubes (MWNT-PDMA) were prepared via atom transfer radical polymerization and then added into quasi-interpenetrating network (quasi-IPN) composed of linear polyacrylamide (3.3 MDa) and PDMA to form polymer/nanotube double-network composite sieving matrices for DNA sequencing by CE. The CE results show that, compared with quasi-IPN, the novel composite matrices can improve ssDNA sequencing performances due to the formation of a double-network consisting of a flexible quasi-IPN polymer network and a rigid MWNT network based on a unique tubular structure, which makes the total sieving networks more restricted and stable and increases the apparent molecular weight of the matrices. The effects of MWNT-PDMA concentration in matrices and molecular weight of PDMA side chains in MWNT-PDMA on ssDNA sequencing performances were studied in detail. Furthermore, these double-network composite matrices were also compared with other matrices and the results indicate that they are promising ones for DNA sequencing. The separation provided with high resolution, speediness, excellent reproducibility and easy loading owing to the addition of MWNT-PDMA is likely to achieve full automation, especially for capillary array electrophoresis and microchip electrophoresis.
Zhou
Dan
D
Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, PR China.
Yang
Liping
L
Yang
Runmiao
R
Song
Weihua
W
Peng
Shuhua
S
Wang
Yanmei
Y
D000178:Acrylamides / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000235:genetics / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000648:ultrastructure; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
D000178:Acrylamides; D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; C429790:poly(N,N-dimethylacrylamide)