Effective purification of ginsenosides from cultured wild ginseng roots,red ginseng, and white ginseng with macroporous resins.
This study was aimed (i) to develop an effective method for the purification of ginsenosides for industrial use and (ii) to compare the distribution of ginsenosides in cultured wild ginseng roots (adventitious root culture of Panax ginseng) with those of red ginseng (steamed ginseng) and white ginseng (air-dried ginseng). The crude extracts of cultured wild ginseng roots, red ginseng, and white ginseng were obtained by using a 75% ethanol extraction combined with ultrasonication. This was followed sequentially by AB-8 macroporous adsorption chromatography, Amberlite IRA 900 Cl anion-exchange chromatography, and Amberlite XAD16 adsorption chromatography for further purification. The contents of total ginsenosides were increased from 4.1%, 12.1%, and 11.3% in the crude extracts of cultured wild ginseng roots, red ginseng, and white ginseng to 79.4%, 71.7%, and 72.5% in the final products, respectively. HPLC analysis demonstrated that ginsenosides in cultured wild ginseng roots were distributed in a different ratio compared with red ginseng and white ginseng.
Li
Huayue
H
Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University, Busan 617-736, Korea.
Lee
Jae-Hwa
JH
Ha
Jong-Myung
JM
D001709:Biotechnology / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification*; D007475:Ion Exchange Resins; D005894:Panax / Q000737:chemistry* / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry* / Q000254:growth & development; D012117:Resins, Synthetic
D036145:Ginsenosides; D007475:Ion Exchange Resins; D010936:Plant Extracts; D012117:Resins, Synthetic; C046797:amberlite