Identification of 2-[2-nitro-4-(trifluoromethyl)benzoyl]- cyclohexane-1,3-dione metabolites in urine of patients suffering from tyrosinemia type I with the use of 1H and 19F NMR spectroscopy.
Organic extracts of six urine samples from children treated with nitisinone, a medicine against tyrosinemia type I, were investigated by (1)H and (19)F NMR spectroscopy. The presence of unchanged 2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione (NTBC), 6-hydroxy-2-[2-nitro-4-(trifluoromethyl)benzoyl]cyclohexane-1,3-dione (NTBC-OH) and 2-nitro-4-trifluoromethylbenzoic acid (NTFA) as well as a few other unidentified compounds containing CF(3) group was documented.
Szczeciński
Przemysław
P
Faculty of Chemistry, Warsaw University of Technology, Warszawa, Poland. przemsz@ch.pw.edu.pl
Lamparska
Diana
D
Gryff-Keller
Adam
A
Gradowska
Wanda
W
D002648:Child; D002675:Child, Preschool; D003512:Cyclohexanones / Q000737:chemistry*; D005260:Female; D005461:Fluorine; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009579:Nitrobenzoates / Q000737:chemistry*; D011522:Protons; D020176:Tyrosinemias / Q000652:urine*
D003512:Cyclohexanones; D009579:Nitrobenzoates; D011522:Protons; D005461:Fluorine; C077073:nitisinone