Infrared imaging contribution for intestinal ischemia detection in wound healing.
OBJECTIVE
To study thermal variations obtained through infrared image in rats, and to evaluate the relationship between intestinal ischemic time and histopathological findings.


METHODS
Thirty Wistar rats were operated after distribution in 5 groups with different times of ischemia. Thermograms were obtained by using a infrared camera. The surgical technique has been standardized for all groups: abdominal cavity opening by a 5 cm length incision in the midline, abdominal wall plans section and cavity exposure, and exteriorization of the intestine. In group I (control), it was proceeded only laparotomy without superior mesenteric artery ligature. After first thermogram done, incision was closed with continuing suture. In each rat in groups II, III, IV and V, the superior mesenteric artery was located at its origin on abdominal aorta, dissected and occluded with a vascular microclamp, subjecting the intestine to ischemia in variable times.


RESULTS
Rats submitted to a 30 minutes ischemia presented reactive hyperemia, thermal differential of 1.8 degrees C and normal pathological examination. The 1 hour ischemia produced reactive hyperemia with ischemic areas, thermal differential of 1.0 degrees C and injuries at villosities' tips. However, the 90 minutes ischemia had not shown reactive hyperemia with large ischemic areas, thermal differential of -1.0 degrees C and injury in the upper third of the villosities. The 2 hours ischemia demonstrated a severe ischemia, thermal differential of -2.0 degrees C and injury throughout the all villosities' extension.


CONCLUSIONS
It has been possible studying thermal variations through infrared image in rats, showing correlation between thermal response in thermograms, ischemic time and histopathological findings.
Malafaia
Osvaldo
O
FEPAR, Curitiba - PR, Brazil.
Brioschi
Marcos Leal
ML
Aoki
Sonia Maria Schneider
SM
Dias
Fernando Gallego
FG
Gugelmin
Bruno Schneider
BS
Aoki
Massao Schneider
MS
Aoki
Yuki Schneider
YS
D000704:Analysis of Variance; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D004195:Disease Models, Animal; D006940:Hyperemia / Q000175:diagnosis / Q000503:physiopathology; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D008638:Mesenteric Arteries / Q000601:surgery; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013055:Spectrophotometry, Infrared; D013817:Thermography / Q000379:methods*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*