title: Mucosal prior to systemic application of recombinant adenovirus boosting is more immunogenic than systemic application twice but confers similar protection against SIV-challenge in DNA vaccine-primed macaques.
abstract: We investigated the immunogenicity and efficacy of a bimodal prime/boost vaccine regimen given by various routes in the Simian immunodeficiency virus (SIV) rhesus monkey model for AIDS. Twelve animals were immunized with SIV DNA-vectors followed by the application of a recombinant adenovirus (rAd5) expressing the same genes either intramuscularly (i.m.) or by oropharyngeal spray. The second rAd5-application was given i.m. All vaccinees plus six controls were challenged orally with SIVmac239 12 weeks post-final immunization. Both immunization strategies induced strong SIV Gag-specific IFN-gamma and T-cell proliferation responses and mediated a conservation of CD4(+) memory T-cells and a reduction of viral load during peak viremia following infection. Interestingly, the mucosal group was superior to the systemic group regarding breadth and strength of SIV-specific T-cell responses and exhibited lower vector specific immune responses. Therefore, our data warrant the inclusion of mucosal vector application in a vaccination regimen which makes it less invasive and easier to apply.
authors.affiliation: Working group Infection Models, German Primate Center, Kellnerweg, Goettingen, Germany.
authors.forename: Reiner
authors.initials: R
authors.lastname: Schulte
authors.forename: You-Suk
authors.initials: YS
authors.lastname: Suh
authors.forename: Ulrike
authors.initials: U
authors.lastname: Sauermann
authors.forename: Washingtone
authors.initials: W
authors.lastname: Ochieng
authors.forename: Sieghart
authors.initials: S
authors.lastname: Sopper
authors.forename: Kwang S
authors.initials: KS
authors.lastname: Kim
authors.forename: So-Shin
authors.initials: SS
authors.lastname: Ahn
authors.forename: Ki S
authors.initials: KS
authors.lastname: Park
authors.forename: Nicole
authors.initials: N
authors.lastname: Stolte-Leeb
authors.forename: Gerhard
authors.initials: G
authors.lastname: Hunsmann
authors.forename: Young C
authors.initials: YC
authors.lastname: Sung
authors.forename: Christiane
authors.initials: C
authors.lastname: Stahl-Hennig
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D049109:Cell Proliferation; D007114:Immunization / Q000379:methods*; D007117:Immunization, Secondary / Q000379:methods*; D007156:Immunologic Memory; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000378:metabolism; D008253:Macaca mulatta; D009960:Oropharynx / Q000276:immunology; D018449:SAIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology; D019562:Viral Load; D014766:Viremia / Q000517:prevention & control
chemical_list: D018449:SAIDS Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma