[Effects of hnRNP B1-specific siRNA on the expression of hnRNP B1 in lung cancer tissues in vivo].
OBJECTIVE
To measure the effect of the eukaryotic expression vector of siRNA specific for hnRNP B1 on the expression of hnRNP Bx in lung cancer tissues in vivo.


METHODS
The eukaryotic expression vector of siRNA specific for hnRNP B1 was inoculated to nude mice with lung cancers. The mRNA and protein expressions of hnRNP B1 in the lung cancer tissues of the nude mice were detected by RT-PCR and Western blot. The optimal conditions for the expressions were identified.


RESULTS
The eukaryotic expression vector of siRNA specific for hnRNP B1 inhibited the mRNA and protein expressions at 40 days after the inoculation. However, the inhibitive effect disappeared at 60 days after the inoculation.


CONCLUSIONS
The expression of hnRNP B1 gene in A549 cells can be inhibited by hnRNP Br-specific siRNA expression vectors.
Wen
Chun-sheng
CS
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Wei-min
WM
Pu
Dan
D
Chen
Wen-bing
WB
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D005057:Eukaryotic Cells / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C439768:hnRNP A2