Apoptosis changes and SA-beta galactosidase expression in stress-induced premature senescence (SIPS) model of human skin fibroblasts.
Stress-induced premature senescence (SIPS) model is in vitro model of cellular aging. In this study, apoptosis was evaluated in SIPS model and in replicative senescent fibroblasts. We also compared the activity of senescence-associated beta-galactosidase (SA-beta gal) as a biomarker of cellular aging. Our results suggested that SIPS model and senescent fibroblasts might share similar mechanism of aging and apoptosis pathway.
Abdul Rahim
N
N
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Makpol
S
S
Chua
K H
KH
Yusof
Y A Mohd
YA
Top
G Md
GM
Ngah
W Z Wan
WZ
D000375:Aging / Q000187:drug effects / Q000502:physiology*; D017302:Annexins; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers; D016922:Cellular Senescence / Q000187:drug effects*; D002944:Circumcision, Male; D005347:Fibroblasts / Q000187:drug effects*; D052816:Foreskin / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D008297:Male; D008962:Models, Theoretical; D012867:Skin / Q000187:drug effects*; D012879:Skin Physiological Phenomena / Q000187:drug effects*; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000187:drug effects
D017302:Annexins; D015415:Biomarkers; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase