title: Evaluation of an immunochromatographic assay kit for rapid identification of Mycobacterium tuberculosis complex in clinical isolates.
abstract: We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Park
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Hwang
authors.forename: Hyoung Hoi
authors.initials: HH
authors.lastname: Kim
authors.forename: Eun Yup
authors.initials: EY
authors.lastname: Lee
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Chulhun L
authors.initials: CL
authors.lastname: Chang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000911:Antibodies, Monoclonal