Development of an immunoglobulin M capture-based enzyme-linked immunosorbent assay for diagnosis of acute infections with Bartonella henselae.
We describe the development of an immunoglobulin M-specific enzyme-linked immunosorbent assay for the detection of an early antibody response to Bartonella henselae, the causative agent of cat scratch disease, bacillary angiomatosis, and endocarditis. This assay discriminates between B. henselae-positive and -negative patient samples with sensitivity and specificity values of 100% and 97.1%, respectively.
Hoey
John G
JG
Antigen Discovery Division, Research and Development Department, Medical Diagnostic Laboratories, Hamilton, New Jersey 08690-3303, USA. jhoey@mdlab.com
Valois-Cruz
Fernando
F
Goldenberg
Hannah
H
Voskoboynik
Yekaterina
Y
Pfiffner
Jenna
J
Tilton
Richard C
RC
Mordechai
Eli
E
Adelson
Martin E
ME
D016917:Angiomatosis, Bacillary / Q000175:diagnosis / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D018416:Bartonella henselae / Q000276:immunology / Q000302:isolation & purification*; D002372:Cat-Scratch Disease / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D012680:Sensitivity and Specificity; D055815:Young Adult
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M