title: Short term effects of doxycycline on matrix metalloproteinases 2 and 9.
abstract: OBJECTIVE
  To investigate the short term effects of Doxycycline on MMP-2 and MMP-9.
  
  
  METHODS
  Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells.
  
  
  RESULTS
  In plasma, MMP-9 activity was reduced (-22%, 95% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28%, 95% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52%, 95% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively).
  
  
  CONCLUSIONS
  Doxy decreases MMP-9 plasma levels by around 20%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.
authors.affiliation: S C Clinica Medica Generale e Terapia Medica, Dipartimento di Scienze Cliniche, Morfologiche e Tecnologiche, Università degli Studi di Trieste, Trieste, Italy. fiotti@units.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Fiotti
authors.forename: Nicola
authors.initials: N
authors.lastname: Altamura
authors.forename: Michèle
authors.initials: M
authors.lastname: Moretti
authors.forename: Stella
authors.initials: S
authors.lastname: Wassermann
authors.forename: Serena
authors.initials: S
authors.lastname: Zacchigna
authors.forename: Rossella
authors.initials: R
authors.lastname: Farra
authors.forename: Barbara
authors.initials: B
authors.lastname: Dapas
authors.forename: Lara
authors.initials: L
authors.lastname: Consoloni
authors.forename: Mauro
authors.initials: M
authors.lastname: Giacca
authors.forename: Gabriele
authors.initials: G
authors.lastname: Grassi
authors.forename: Carlo
authors.initials: C
authors.lastname: Giansante
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D004318:Doxycycline / Q000494:pharmacology*; D004656:Enalapril / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D009566:Nitrates / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D009566:Nitrates; D004656:Enalapril; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D004318:Doxycycline; D001241:Aspirin