Disposable electrochemical immunosensor for carcinoembryonic antigen using ferrocene liposomes and MWCNT screen-printed electrode.
Disposable electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) in saliva and serum was developed. Monoclonal anti-CEA antibodies (alphaCEA) were covalently immobilized on polyethyleneimine wrapped multiwalled carbon nanotubes screen-printed electrode. A sandwich immunoassay was performed with CEA and alphaCEA tagged ferrocene carboxylic acid encapsulated liposomes (alphaCEA-FCL). The square wave voltammetry (SWV) was employed to analyze faradic redox responses of the released ferrocene carboxylic acid from the immunoconjugated liposomes on the electrode surface. The magnitude of the SWV peak current was directly related to the concentration of CEA. The calibration curve for CEA concentration was in the range of 5 x 10(-12) to 5 x 10(-7)gmL(-1) with a detection limit of 1 x 10(-12)gmL(-1) (S/N=3). This method provides a high precise and sensitive determination of CEA in human blood serum and saliva samples.
Viswanathan
Subramanian
S
Department of Biosensors, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
Rani
Chinnakkaruppanan
C
Vijay Anand
Annadurai
A
Ho
Ja-An Annie
JA
D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D004209:Disposable Equipment; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005296:Ferrous Compounds / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008081:Liposomes / Q000737:chemistry; D000075163:Metallocenes; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002272:Carcinoembryonic Antigen; D005296:Ferrous Compounds; D008081:Liposomes; D000075163:Metallocenes; D037742:Nanotubes, Carbon; C004998:ferrocene