Portal venous arterialization resulting in increased portal inflow and portal vein wall thickness in rats.
OBJECTIVE
To explore the influence of portal vein hemo-dynamic changes after portal venous arterialization (PVA) on peribiliary vascular plexus (PVP) morphological structure and hepatic pathology, and to establish a theoretical basis for the clinical application of PVA.


METHODS
Sprague-Dawley rats were randomly divided into control and PVA groups. After PVA, hemodynamic changes of the portal vein and morphological structure of hepatohilar PVP were observed using Doppler ultrasound, liver function tests, ink perfusion transparency management and three-dimensional reconstruction of computer microvisualization, and pathological examination was performed on tissue from the bile duct wall and the liver.


RESULTS
After PVA, the cross-sectional area and blood flow of the portal vein were increased, and the increase became more significant over time, in a certain range. If the measure to limit the flow in PVA was not adopted, the high blood flow would lead to dilatation of intrahepatic portal vein and its branches, increase in collagen and fiber degeneration in tunica intima. Except glutamic pyruvic transaminase (GPT), other liver function tests were normal.


CONCLUSIONS
Blood with a certain flow and oxygen content is important for filling the PVP and meeting the oxygen requirement of the bile duct wall. After PVA, It is the anatomic basis to maintain normal morphology of hepatohilar bile duct wall that the blood with high oxygen content and high flow in arterialized portal vein may fill PVP by collateral vessel reflux. A adequate measure to limit blood flow is necessary in PVA.
Li
Wen-Gang
WG
Institute of Hepatobiliary Surgery, General Hospital of PLA, Beijing 100853, China.
Chen
Yong-Liang
YL
Chen
Jing-Xi
JX
Qu
Lei
L
Xue
Bin-Dang
BD
Peng
Zhi-Hai
ZH
Huang
Zhi-Qiang
ZQ
D000818:Animals; D006499:Hepatic Artery / Q000502:physiology / Q000601:surgery; D008099:Liver / Q000098:blood supply / Q000502:physiology; D008102:Liver Circulation / Q000502:physiology; D008297:Male; D023421:Models, Animal; D010100:Oxygen / Q000097:blood; D011168:Portal System / Q000601:surgery*; D011169:Portal Vein / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D017539:Tunica Intima / Q000033:anatomy & histology*; D014656:Vascular Surgical Procedures / Q000379:methods*
D010100:Oxygen