title: On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles.
abstract: As a step toward a fully automated front-end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI-MS, using a replaceable on-chip digestion microcolumn based on self-assembled magnetic particles.
authors.affiliation: Laboratoire PCC, Institut Curie, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Le Nel
authors.forename: Jana
authors.initials: J
authors.lastname: Krenkova
authors.forename: Karel
authors.initials: K
authors.lastname: Kleparnik
authors.forename: Claire
authors.initials: C
authors.lastname: Smadja
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
authors.forename: Frantisek
authors.initials: F
authors.lastname: Foret
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D014357:Trypsin / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; D011506:Proteins; C013830:baysilon; D014357:Trypsin