A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping.
In a haematology ward, Candida parapsilosis was found in blood cultures from 4 patients within a month. As C. parapsilosis is known to have a restricted genetic diversity, a combined methodological approach was adopted to establish a possible epidemiological relationship among the isolates (n = 9). Multilocus sequence typing and random amplified polymorphic DNA analysis suggested a clonal origin of the isolates. The clonal origin was confirmed by microsatellite analysis, a method that displayed the highest discriminatory level and readily differentiated cluster isolates from 2 epidemiologically unrelated strains of C. parapsilosis. The use of novel methods of genotyping such as microsatellite analysis will facilitate epidemiological investigations of potential clonal outbreaks of fungaemia.
Brillowska-Dabrowska
Anna
A
Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark.
Schön
T
T
Pannanusorn
S
S
Lönnbro
N
N
Bernhoff
L
L
Bonnedal
J
J
Haggstrom
J
J
Wistedt
A
A
Fernandez
V
V
Arendrup
M C
MC
D000368:Aged; D000369:Aged, 80 and over; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000453:epidemiology* / Q000382:microbiology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004271:DNA, Fungal / Q000235:genetics*; D004196:Disease Outbreaks; D005260:Female; D016469:Fungemia / Q000382:microbiology; D005821:Genetic Techniques; D005838:Genotype; D006757:Hospital Units; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013548:Sweden / Q000453:epidemiology
D004271:DNA, Fungal