title: Serological survey of domestic animals for tick-borne encephalitis and Bhanja viruses in northeastern Hungary.
abstract: Blood sera collected from 400 domestic animals (260 cattle, 100 Merino sheep, and 40 Hutzul horses) in northeastern Hungary in 2005 were examined for antibodies against two tick-borne viruses, tick-borne encephalitis flavivirus (TBEV) and Bhanja bunyavirus (BHAV). Using ELISA as screening test and plaque-reduction neutralization as confirmatory test, seropositivity to TBEV was found to be 26.5% in cattle, 7.0% in sheep, and 0.0% in horses. Among cattle, the animals up to 3 years old had significantly lower seroprevalence rate than those in older age groups. Natural foci of tick-borne encephalitis are obviously present in northeastern Hungary. On the other hand, no antibodies neutralizing BHAV were detected in the domestic animals.
authors.affiliation: Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Medical Zoology Laboratory, Kvetná 8, Brno 60365, Czech Republic. sikutova@ivb.cz
authors.forename: Silvie
authors.initials: S
authors.lastname: Sikutová
authors.forename: Sándor
authors.initials: S
authors.lastname: Hornok
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Hubálek
authors.forename: Iva
authors.initials: I
authors.lastname: Dolezálková
authors.forename: Zina
authors.initials: Z
authors.lastname: Juricová
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004669:Encephalitis Viruses, Tick-Borne / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000453:epidemiology* / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005843:Geography; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D006814:Hungary / Q000453:epidemiology; D009500:Neutralization Tests; D016851:Orthobunyavirus / Q000302:isolation & purification*; D012703:Serotyping; D012757:Sheep Diseases / Q000453:epidemiology / Q000821:virology*; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral