Investigating the mechanisms underlying neuronal death in ischemia using in vitro oxygen-glucose deprivation: potential involvement of protein SUMOylation.
It is well established that brain ischemia can cause neuronal death via different signaling cascades. The relative importance and interrelationships between these pathways, however, remain poorly understood. Here is presented an overview of studies using oxygen-glucose deprivation of organotypic hippocampal slice cultures to investigate the molecular mechanisms involved in ischemia. The culturing techniques, setup of the oxygen-glucose deprivation model, and analytical tools are reviewed. The authors focus on SUMOylation, a posttranslational protein modification that has recently been implicated in ischemia from whole animal studies as an example of how these powerful tools can be applied and could be of interest to investigate the molecular pathways underlying ischemic cell death.
Cimarosti
Helena
H
MRC Centre for Synaptic Plasticity, Department of Anatomy, University Walk, University of Bristol, Bristol, UK.
Henley
Jeremy M
JM
D000818:Animals; D016923:Cell Death / Q000502:physiology; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000473:pathology; D006801:Humans; D000860:Hypoxia / Q000150:complications*; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D008962:Models, Theoretical; D009474:Neurons / Q000502:physiology*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000502:physiology*
D025841:Small Ubiquitin-Related Modifier Proteins; D005947:Glucose