Genetic variation in wild and cultivated rhizoma corydalis revealed by ISSRs markers.
Rhizoma Corydalis is an important Chinese medicinal herb. In this paper, we employed ISSR data to explore the genetic variation in domesticated populations and wild populations of the species. The average of within-population ISSR diversity in cultivated populations (PPF=25.32%, Hpop=0.094) was lower than that in wild populations (PPF=47.70%, Hpop=0.144). Cultivated populations (PhiST=0.515, GST=0.429) have a greater proportion of their genetic variability distributed among populations than wild populations (PhiST=0.277, GST=0.226). Based on hierarchical estimates of variance components, significant statistical differences (57.77%, P<0.001) were found between the wild and cultivated groups. The low levels of genetic diversity within cultivated populations and high levels of genetic differentiation among populations/groups may result from artificial selection, the mode of clonal propagation, and only limited exchange of material among localities. Finally, some suggestions for conservation and efficient management of the genetic resources of this important medicinal herb are proposed.
Qiu
Ying-Xiong
YX
Laboratory of Systematic and Evolutionary Botany, and State Conservation Center for Gene Resources of Endangered Wildlife, College of Life Sciences, Zhejiang University, Hangzhou, China.
Zong
Min
M
Yao
Yu
Y
Chen
Bin-Long
BL
Zhou
Xiao-Long
XL
Chen
Zi-Lin
ZL
Fu
Cheng-Xin
CX
D031311:Corydalis / Q000235:genetics*; D005819:Genetic Markers; D018598:Minisatellite Repeats; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic
D005819:Genetic Markers