title: Protective effect of resveratrol on acute endotoxemia-induced nephrotoxicity in rat through nitric oxide independent mechanism.
abstract: Lipopolysaccharide (LPS) is a glycolipid component of the cell wall of gram negative bacteria inducing deleterious effects on the kidney. Endotoxemia-induced nephrotoxicity is characterized by disturbed intracellular redox balance and reactive oxygen species (ROS) accumulation leading to DNA, proteins and membrane lipid damages. Resveratrol (trans-3,5,4'-trihydroxystilbene) is a polyphenol displaying antioxidant and anti-inflammatory properties. This study investigated its effects on LPS-induced nephrotoxicity in rats. Resveratrol counteracted all LPS-induced changes in renal haemodynamic parameters. In the kidney resveratrol abrogated LPS-induced lipoperoxidation and antioxidant enzyme activities depletion as superoxide dismutase (SOD) and catalase (CAT) but not peroxidase (POD) activity. LPS increased plasma and urine nitric oxide (NO) level and resveratrol reversed them. More importantly, LPS-induced iron mobilization from plasma to kidney, which was also abolished by resveratrol treatment. All these results suggest that resveratrol exerted strong antioxidant properties against LPS-induced nephrotoxicity and that its mode of action seemed to involve iron shuttling proteins.
authors.affiliation: UR Ethnobotanie & Stress Oxydant, Departement des Sciences de la Vie, Faculte des Sciences de Bizerte, Zarzouna, Tunisie.
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
authors.forename: Mossadok
authors.initials: M
authors.lastname: Ben-Attia
authors.forename: Mamane
authors.initials: M
authors.lastname: Sani
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Aouani
authors.forename: Néziha
authors.initials: N
authors.lastname: Ghanem-Boughanmi
mesh_terms: D000818:Animals; D019446:Endotoxemia / Q000378:metabolism / Q000503:physiopathology*; D007674:Kidney Diseases / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*; D007677:Kidney Function Tests; D015227:Lipid Peroxidation; D008070:Lipopolysaccharides; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D013267:Stilbenes; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D000077185:Resveratrol