title: Frequency and distribution of Epstein-Barr virus infection and its association with P53 expression in a series of primary nodal non-Hodgkin lymphoma patients from South India.
abstract: This study investigated the prevalence of Epstein-Barr virus (EBV) infection and its association with P53 expression in a panel of 87 previously untreated nodal non-Hodgkin lymphomas (NHLs) from India. Polymerase chain reaction specific for Epstein Barr nuclear antigen 1 (EBNA1) and EBNA-3C was performed on the lymphnode tissue DNA, while P53 expression was analyzed by immunohistochemistry. EBV, predominantly type A strain, was detected in 27/87 (31%) nodal lymphoid malignancies, 11/46 diffuse large B-cell lymphomas, 6/17 follicular lymphoma, 4/6 anaplastic large cell lymphomas (ALCL), 5/11 peripheral T-cell lymphomas (PTCL) and 1/7 lymphoblastic lymphomas. EBV infection was more frequently observed in a specific subset of nodal NHL, suggesting a causative role of EBV infection in the pathogenesis of ALCL and PTCL. There was no significant association between EBV and P53 expression in our series of NHL patients.
authors.affiliation: Department of Molecular Oncology, Cancer Institute (WIA), Chennai, India.
authors.forename: P S
authors.initials: PS
authors.lastname: Sasikala
authors.forename: K
authors.initials: K
authors.lastname: Nirmala
authors.forename: S
authors.initials: S
authors.lastname: Sundersingh
authors.forename: U
authors.initials: U
authors.lastname: Mahji
authors.forename: T
authors.initials: T
authors.lastname: Rajkumar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007194:India; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000821:virology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D017421:Sequence Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens; D016159:Tumor Suppressor Protein p53; C417029:EBV-encoded nuclear antigen 1