Proteomic analysis of HepaRG cells: a novel cell line that supports hepatitis B virus infection.
The first proteomic characterization of the HepaRG cell line, the only cell line that is susceptible to hepatitis B virus (HBV) infection and supports a complete virus life cycle, is reported. Differential analysis of naive and HBV-infected HepaRG cells by two-dimensional gel electrophoresis revealed 19 differentially regulated features, 7 increasing and 12 decreasing with HBV infection. The proteins identified in these features were involved in various cellular pathways including apoptosis, DNA/RNA processing, and hepatocellular impairment. Similar expression changes in a number of the identified proteins have already been reported for other virus systems. Identification of these expression changes is a validation of the proteomics approach and contributes to an understanding of host cellular response to HBV infection.
Narayan
Ramamurthy
R
Oxford Antiviral Drug Discovery Unit, Oxford, OX1 3QU, UK. narayan.ramamurthy@ndm.ox.ac.uk
Gangadharan
Bevin
B
Hantz
Olivier
O
Antrobus
Robin
R
García
Angela
A
Dwek
Raymond A
RA
Zitzmann
Nicole
N
D017209:Apoptosis; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006509:Hepatitis B / Q000378:metabolism*; D006515:Hepatitis B virus / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D013058:Mass Spectrometry; D010641:Phenotype; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000737:chemistry
D020543:Proteome; D012313:RNA; D004247:DNA