title: Effects of VEGF and FGF2 on the revascularization of severed human dental pulps.
abstract: The long-term outcome of replanted avulsed permanent teeth is frequently compromised by lack of revascularization, resulting in pulp necrosis. The purpose of this study was to evaluate the effects of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF-2) on the revascularization of severed human dental pulps. Tooth slices were prepared from non-carious human molars and treated with 0-50 ng/mL rhVEGF(165) or rhFGF-2 for 7 days in vitro. Both angiogenic factors enhanced pulp microvessel density compared with untreated controls (p < 0.05). Tooth slices were also treated with 0 or 50 ng/mL rhVEGF(165) for one hour prior to implantation into the subcutaneous space of immunodeficient mice. Treatment with rhVEGF(165) increased pulp microvessel density in vivo (p < 0.05). These results demonstrate that rhVEGF(165) enhanced neovascularization of severed human dental pulps and suggest that topical application of an angiogenic factor prior to replantation might be beneficial for the treatment of avulsed teeth.
authors.affiliation: Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, 1011 N. University, Ann Arbor, MI 48109-1078, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Mullane
authors.forename: Z
authors.initials: Z
authors.lastname: Dong
authors.forename: C M
authors.initials: CM
authors.lastname: Sedgley
authors.forename: J C-C
authors.initials: JC
authors.lastname: Hu
authors.forename: T M
authors.initials: TM
authors.lastname: Botero
authors.forename: G R
authors.initials: GR
authors.lastname: Holland
authors.forename: J E
authors.initials: JE
authors.lastname: Nör
mesh_terms: D000293:Adolescent; D000328:Adult; D042501:Angiogenic Proteins / Q000494:pharmacology*; D000818:Animals; D003470:Culture Media; D003782:Dental Pulp / Q000098:blood supply / Q000187:drug effects / Q000293:injuries* / Q000637:transplantation; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D055806:Microvessels / Q000187:drug effects / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D040521:Subcutaneous Tissue / Q000601:surgery; D013997:Time Factors; D046509:Tissue Culture Techniques; D019737:Transplants; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D042501:Angiogenic Proteins; D003470:Culture Media; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2