title: Evidence for intestinal heterogenic expression of di-tripeptides transporter PepT1 during experimental cryptosporidiosis in neonatal rats.
abstract: Cryptosporidium parvum is a protozoan parasite that causes intestinal malabsorptive syndrome and malnutrition. Considering the importance of di-tripeptide absorption for nutritional status, we previously investigated the regulation of PepT1 transporter in the suckling rat model of acute cryptosporidiosis and showed that PepT1 protein expression and activity were not modified in the parasitized intestine. Here we used confocal microscopy performed on intestinal villi to determine the subcellular localization of PepT1 together with f-actin and parasites. For this purpose, confocal microscopy using vibratome thick sections was developed on the distal small intestine, the preferential site of parasite implantation. Results showed major heterogeneity of apical PepT1 expression among enterocytes, which did not correlate with actin staining or parasite implantation. These results underscore the importance of considering the effect of C. parvum at the cellular scale and not only in the entire epithelium.
authors.affiliation: EA209 Eucaryotes pathogènes: transports membranaires et chimiorésistance, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 75006, Paris, France.
authors.forename: Perrine
authors.initials: P
authors.lastname: Marquet
authors.forename: Bruno
authors.initials: B
authors.lastname: Saubaméa
authors.forename: Leila
authors.initials: L
authors.lastname: Snouber-Choucha
authors.forename: Valérie
authors.initials: V
authors.lastname: Gafa
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kapel
authors.forename: Laurence
authors.initials: L
authors.lastname: Barbot-Trystram
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000737:chemistry / Q000502:physiology*; D005260:Female; D007413:Intestinal Mucosa / Q000469:parasitology; D026901:Membrane Transport Proteins / Q000096:biosynthesis*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D051381:Rats
chemical_list: D000199:Actins; D026901:Membrane Transport Proteins; C043912:peptide permease