title: Variation in the replication loci of Streptomyces linear plasmids.
abstract: Streptomyces linear plasmids start replication at centrally located loci, usually consisting of iterons and adjacent rep genes. Here, we identified four new replication loci from Streptomyces linear plasmids. A discontinuous locus, consisting of two genes and iterons separated by two nonessential genes, was required for replication of pRL2 in both linear and circular modes. A temperature-sensitive plasmid, pRL4, contained a replication locus, a noncoding sequence and a SAP1.35-like gene. A telomere-adjacent locus, another noncoding sequence and SAP1.1-like gene, was identified for replication of the large plasmid pFRL2. The replication locus of pSHK1 consisted of SCP1-rep-like genes and iterons. These results indicate an unexpected variety of components, positions and combinations of replication loci among Streptomyces linear plasmids.
authors.affiliation: Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.forename: Haiyang
authors.initials: H
authors.lastname: Xia
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Qin
mesh_terms: D004261:DNA Replication; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin; D013302:Streptomyces / Q000235:genetics*