title: Matrix proteins of basement membrane of intrahepatic bile ducts are degraded in congenital hepatic fibrosis and Caroli's disease.
abstract: Congenital hepatic fibrosis (CHF) and Caroli's disease are though to result from ductal plate malformation, and the basal laminar components play important roles in biliary differentiation during development. To clarify the involvement of basal laminar components in the ductal plate malformation, this study examined the immunohistochemical expression of laminin and type IV collagen in the livers of CHF and Caroli's disease. Using the polycystic kidney (PCK) rat, an animal model of Caroli's disease with CHF, in vivo and in vitro experiments were also performed. Immunostaining showed that basement membrane expression of laminin and type IV collagen around intrahepatic bile ducts was degraded in CHF, Caroli's disease, and the PCK rats. The degradation of laminin and type IV collagen around bile ducts was also observed in foci of cholangiocarcinoma in situ of Caroli's disease. In vitro, PCK cholangiocytes were found to overexpress plasminogen and a serine proteinase, the tissue-type plasminogen activator (tPA). When PCK cholangiocytes were cultured in Matrigel, the amounts of laminin and collagen in the gel were significantly reduced, and addition of alpha2-antiplasmin in the culture medium inhibited the degradation of laminin and collagen in Matrigel. These results suggest that biliary overexpression of plasminogen and tPA leads to the generation of excessive amounts of plasmin, and subsequent plasmin-dependent lysis of the extracellular matrix molecules may contribute to the biliary dysgenesis in CHF and Caroli's disease, including progressive cystic dilatation of the intrahepatic bile ducts in Caroli's disease. In addition, it is suggested that once cholangiocarcinoma in situ develops in the biliary epithelium of CHF and Caroli's disease, it tends to transform into invasive carcinoma, due to instability of the basement membrane of the bile ducts.
authors.affiliation: Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa, Japan.
authors.forename: Mitsue
authors.initials: M
authors.lastname: Yasoshima
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Sato
authors.forename: Shinichi
authors.initials: S
authors.lastname: Furubo
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kizawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sanzen
authors.forename: Satoru
authors.initials: S
authors.lastname: Ozaki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Harada
authors.forename: Yasuni
authors.initials: Y
authors.lastname: Nakanuma
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry / Q000378:metabolism*; D016767:Caroli Disease / Q000378:metabolism*; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000032:analysis / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051381:Rats; D011922:Rats, Mutant Strains; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis / Q000378:metabolism
chemical_list: D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D007797:Laminin; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator