Sialic acid residues are essential for the anaphylactic activity of murine IgG1 antibodies.
Glycosylation of the Ab molecule is essential for maintaining the functional structure of Fc region and consequently for Ab-mediated effector functions, such as binding to cells or complement system activation. Alterations in the composition of the sugar moiety can dramatically influence Ab activity; however, it is not completely clear how differences in the N-linked oligosaccharide structure impact the biological function of Abs. We have described that murine IgG1 Abs can be separated according to their ability to elicit in vivo anaphylaxis in a fraction of anaphylactic and other of non-anaphylactic molecules. Furthermore, we showed that the N-linked oligosaccharide chain is essential for the structural conformation of the anaphylactic IgG1, the binding to FcgammaRIII on mast cells, and, consequently, for the ability to mediate anaphylactic reactions. In this study, we evaluated the contribution of individual sugar residues to this biological function. Differences in the glycan composition were observed when we analyzed oligosaccharide chains from anaphylactic or non-anaphylactic IgG1, mainly the presence of more sialic acid and fucose residues in anaphylactic molecules. Interestingly, the enzymatic removal of terminal sialic acid residues in anaphylactic IgG1 resulted in loss of the ability to trigger mast cell degranulation and in vivo anaphylactic reaction, similarly to the deglycosylated IgG1 Ab. In contrast, fucose removal did not affect the anaphylactic function. Therefore, we demonstrated that the ability of murine IgG1 Abs to mediate anaphylaxis is directly dependent on the amount of sialic acid residues associated to the oligosaccharide chain attached to the Fc region of these molecules.
Silva
Sandriana R
SR
Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Casabuono
Adriana
A
Jacysyn
Jacqueline F
JF
Favoretto
Bruna C
BC
Fernandes
Irene
I
Macedo
Mahasti S
MS
Couto
Alicia S
AS
Faquim-Mauro
Eliana L
EL
D000707:Anaphylaxis / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D001666:Binding Sites, Antibody; D002236:Carbohydrate Conformation; D002460:Cell Line; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D013329:Structure-Activity Relationship
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D037102:Lectins; D009844:Oligosaccharides; D012794:Sialic Acids