[Expression level changes of inhibitor of differentiation 1 during ATRA-induced acute promyelocytic leukemia cells differentiation].
OBJECTIVE
To study the role of inhibitor of differentiation 1 (ID1) in ATRA-induced acute promyelocytic leukemia (APL) cells differentiation.


METHODS
The expression of ID1 was detected by cDNA microarray, cycloheximide inhibition test, real-time RT-PCR and western blot.


RESULTS
The expression of ID1 gene was up-regulated in ATRA-induced NB4 cells and APL cells from two patients and was independent on other proteins synthesis. ID1 expression level reached the peak at 2 h in NB4 cells induced by ATRA, its relative expression level was (359.4 +/- 48.7)-fold greater than control. ID1 expression level reached the peak at 2 h in bone marrow cells from APL patents treated with ATRA, and its level detected 3 times in one of the patient was (311.1 +/- 48.7) fold of control. The expression of ID1 protein was not up-regulated in ATRA resistant NB4-R2 cells after ATRA treatment.


CONCLUSIONS
ID1 may be involved in ATRA-induced granulocytic differentiation as an ATRA-targeted gene.
Zheng
Pei-zheng
PZ
Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Zhao
Chun-jun
CJ
Fan
Hui-yong
HY
Chen
Yu-long
YL
D000970:Antineoplastic Agents / Q000627:therapeutic use; D002454:Cell Differentiation; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D014212:Tretinoin / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D051798:Inhibitor of Differentiation Protein 1; D014212:Tretinoin