Synthesis and characterization of chitosan-based hydrogels.
Biocompatible hydrogels based on water-soluble chitosan-ethylene glycol acrylate methacrylate (CS-EGAMA) and polyethylene glycol diamethacrylate (PEGDMA) were synthesized by photopolymerization. Characterization of morphology, weight loss, water state of hydrogel, pH-sensitivity and cytotoxicity were investigated by scanning electron microscopy (SEM), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared (FTIR), swelling test and methylthiazolydiphenyl-tetrazolium bromide (MTT) assay. The results indicated that the hydrogels were sensitive to pH of the medium, no cytotoxicity for L929 and SW1353, satisfactory for the composite to be used in bioapplications.
Li
Qianzhu
Q
State Key Laboratory of Chemical Resource Engineering and Key Lab of Beijing, City on Preparation and Processing of Novel Polymer Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
Yang
Dongzhi
D
Ma
Guiping
G
Xu
Qiang
Q
Chen
Xiangmei
X
Lu
Fengmin
F
Nie
Jun
J
D000818:Animals; D002021:Buffers; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects / Q000528:radiation effects; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D012995:Solubility / Q000187:drug effects / Q000528:radiation effects; D012997:Solvents; D013818:Thermogravimetry; D014867:Water; D014961:X-Ray Diffraction
D002021:Buffers; D020100:Hydrogels; D008689:Methacrylates; D012997:Solvents; D014867:Water; C004919:ethylene dimethacrylate; D048271:Chitosan