A ratiometric fluorescent probe for cysteine and homocysteine displaying a large emission shift.
4-(1H-phenanthro[9,10-d]imidazol-2-yl)benzaldehyde 1 was rationally designed as a novel ratiometric fluorescent probe for cysteine and homocysteine. Upon addition of cysteine or homocysteine, notably, the probe displayed a very large (125 nm) hypsochromic shift in emission due to switching off intramolecular charge transfer. This large emission wavelength shift may allow probe 1 to be employed for quantitatively detecting Cys/Hcy.
Lin
Weiying
W
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China. weiyinglin@hnu.cn
Long
Lingliang
L
Yuan
Lin
L
Cao
Zengmei
Z
Chen
Bingbing
B
Tan
Wen
W
D003545:Cysteine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006710:Homocysteine / Q000032:analysis*; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
C543093:4-(1H-phenanthro(9,10-d)imidazol-2-yl)benzaldehyde; D005456:Fluorescent Dyes; D007093:Imidazoles; D010618:Phenanthrolines; D006710:Homocysteine; D003545:Cysteine