title: SV40 lymphomagenesis in Syrian golden hamsters.
abstract: Simian virus 40 (SV40) isolates differ in oncogenic potential in Syrian golden hamsters following intraperitoneal inoculation. Here we describe the effect of intravenous exposure on tumor induction by SV40. Strains SVCPC (simple regulatory region) and VA45-54(2E) (complex regulatory region) were highly oncogenic following intravenous inoculation, producing a spectrum of tumor types. Three lymphoma cell lines were established; all expressed SV40 T-antigen, were immortalized for growth in culture, and were tumorigenic following transplantation in vivo. New monoclonal antibodies directed against hamster lymphocyte surface antigens are described. The cell lines expressed MHC class II and macrophage markers and were highly phagocytic, indicating a histiocytic origin. Many hamsters that remained tumor-free developed SV40 T-antigen antibodies, suggesting that viral replication occurred. This study shows that route of exposure influences the pathogenesis of SV40-mediated carcinogenesis, that SV40 strain VA45-54(2E) is lymphomagenic in hamsters, that hamster lymphoid cells of histiocytic origin can be transformed in vivo and established in culture, and that reagents to hamster leukocyte differentiation molecules are now available.
authors.affiliation: Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA. amcnees@bcm.edu
authors.forename: Adrienne L
authors.initials: AL
authors.lastname: McNees
authors.forename: Regis A
authors.initials: RA
authors.lastname: Vilchez
authors.forename: Tiffany C
authors.initials: TC
authors.lastname: Heard
authors.forename: Vojtech
authors.initials: V
authors.lastname: Sroller
authors.forename: Connie
authors.initials: C
authors.lastname: Wong
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Herron
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Hamilton
authors.forename: William C
authors.initials: WC
authors.lastname: Davis
authors.forename: Janet S
authors.initials: JS
authors.lastname: Butel
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D000956:Antigens, Viral / Q000235:genetics; D002478:Cells, Cultured; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics; D000882:Haplorhini; D008198:Lymph Nodes / Q000276:immunology; D008223:Lymphoma / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008647:Mesocricetus; D012045:Regulatory Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics / Q000472:pathogenicity*; D014412:Tumor Virus Infections / Q000276:immunology / Q000473:pathology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral