title: Fabrication of a glucose biosensor based on inserted barrel plating gold electrodes.
abstract: We demonstrate here the application of barrel plating gold electrodes for fabricating a new type of disposable amperometric glucose biosensor. It is prepared by inserting two barrel plating gold electrodes onto an injection molding plastic base followed by immobilizing with a bioreagent layer and membrane on the electrode surface. The primary function of barrel plating is to provide an economical way to electroplate manufactured parts. The manufacture procedure is simple and can increase the fabrication precision for automation in mass production. At the two-electrode system, the detection of glucose is linear up to 800 mg/dL (i.e., 44.5 mM, r(2) > 0.99) in pH 7.4 PBS with a sensitivity of 0.71 microA/mM. Excellent sensor-to-sensor reproducibility shows coefficients of variation of only 0.8-1.4% for the detection of 56.5-561.0 mg/dL glucose. In laboratory trials 176 capillary blood samples with a range of 30-572 mg/dL glucose are used to evaluate the clinical application of the biosensor. A good linear correlation is observed between the measured values of the proposed biosensor and laboratory reference. Error grid analysis verifies that the proposed technique is promising in fabricating biosensor strips on a mass scale. As successfully demonstrated by using whole blood glucose as a model analyte, the fabrication technique can extend into other barrel plating noble metal electrodes for various applications.
authors.affiliation: Zensor R&D, BIONIME Corporation, and Department of Chemistry, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Cheng-Teng
authors.initials: CT
authors.lastname: Hsu
authors.forename: Hsieh-Hsun
authors.initials: HH
authors.lastname: Chung
authors.forename: Dong-Mung
authors.initials: DM
authors.lastname: Tsai
authors.forename: Mei-Yen
authors.initials: MY
authors.lastname: Fang
authors.forename: Hung-Chan
authors.initials: HC
authors.lastname: Hsiao
authors.forename: Jyh-Myng
authors.initials: JM
authors.lastname: Zen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005292:Ferricyanides / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005292:Ferricyanides; C007931:hexacyanoferrate III; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose