Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis.
Micromonospora inyoensis produces sisomicin (Sm), an aminoglycoside antibiotic. The gene cluster of sisomicin biosynthesis spanning ca. 47 kb consists of 37 ORFs encoding various proteins for sisomicin biosynthesis, regulation, resistance and transport. The comparative genetic studies on the biosynthetic genes of sisomicin and gentamicin (Gm) reveal a similar biosynthetic route and provide a framework for the future biosynthetic studies.
Hong
Wen-Rong
WR
College of Biological Science and Technology, Fuzhou University, 523 Gongye Rd, Fuzhou City, Fujian, China.
Hong
Wun-Rong
WR
Ge
Mei
M
Zeng
Zhi-Hong
ZH
Zhu
Li
L
Luo
Min-Yu
MY
Shao
Lei
L
Chen
Dai-Jie
DJ
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D023061:Gene Order; D008847:Micromonospora / Q000235:genetics*; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D012853:Sisomicin / Q000096:biosynthesis*; D026801:Synteny
D000900:Anti-Bacterial Agents; D012853:Sisomicin