Transformation of microspore-derived embryos of winter oilseed rape (Brassica napus L.) by using Agrobacterium tumefaciens.
Haploid microspore-derived embryos (MDEs) constitute a unique material for the introduction of new traits into winter oilseed rape (Brassica napus). MDEs have been transformed by using Agrobacterium tumefaciens strains EHA105 and LBA4404, both carrying the binary vector pKGIB containing the uidA gene encoding beta-glucuronidase (GUS) and the bar gene as a marker of resistance to phosphinotricin. Transformed embryos expressed GUS and regenerated plants that were resistant to herbicide Basta, as confirmed by a leaf-painting test. Progeny plants of the transformant T-39 were all transgenic, as they inherited T-DNA from their doubled haploid parental plant. Southern-blot analysis confirmed the integration and transmission of T-DNA into T1 plants. Transformation of MDEs facilitates the obtaining of winter oilseed rape homozygous for the introduced genes.
Cegielska-Taras
Teresa
T
Plant Breeding and Acclimatization Institute, Strzeszyńska 36, 60-479 Poznań, Poland. tceg@nico.ihar.poznan.pl
Pniewski
Tomasz
T
Szała
Laurencja
L
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D029688:Brassica napus / Q000235:genetics*; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D014161:Transduction, Genetic
D005966:Glucuronidase