title: [Immunosuppression induced by measles virus in adult patients is not related to CD4+ CD25+ regulatory T cell induction].
abstract: OBJECTIVE
  To investigate of the relationship of the immunosuppression induced by Measles virus in adult patients and CD4+ CD25+ regulatory T cell.
  
  
  METHODS
  Thirty-four patients with measles and 27 healthy control subjects were included in this study. The whole blood was collected and CD4+ CD25+ cell and FoxP3+ cell were analyzed by flow cytometry, and CD4+ CD25- and CD4+ CD25+ T lymphocytes were isolated from PBMCs of patients with measles or healthy donors, CD4+ CD25- T cells were cultured in absence or presence of anti-CD3, or BCG, or live attenuated MV. The cell culture supernatant was collected after 72 hours and the concentration of IFN-gamma and IL-10 was determined.
  
  
  RESULTS
  Compared to healthy donors, we observed a reduction of the number of white blood cells and lymphocytes in patients with measles, but there was not significantly different in the frequency of CD4+ CD25+ T cells and CD4+ CD25high T cells within the total CD4+ population in the blood. Treg from both measles patients and healthy controls significantly inhibited IFN-gamma production by CD4+ CD25- T cells in response to anti-CD3 stimulation.
  
  
  CONCLUSIONS
  Induction and expansion of Treg may not represent a mechanism involved in the establishment of immune suppression by MV.
authors.affiliation: Shenzhen Donghu Hospital, Shenzhen 518020, China.
authors.forename: Mei-Zhong
authors.initials: MZ
authors.lastname: Li
authors.forename: Fu-De
authors.initials: FD
authors.lastname: Xu
authors.forename: Xue-Huan
authors.initials: XH
authors.lastname: Huang
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Qi-Wen
authors.initials: QW
authors.lastname: Deng
authors.forename: Shui-Teng
authors.initials: ST
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Liu-Mei
authors.initials: LM
authors.lastname: Xu
authors.forename: Huo-Sheng
authors.initials: HS
authors.lastname: Wang
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Cui
mesh_terms: D000293:Adolescent; D000328:Adult; D015704:CD4 Antigens / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008457:Measles / Q000276:immunology* / Q000821:virology; D008459:Measles virus / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
chemical_list: D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit