title: [Three-step purification of preparative-scale antiCD20 (Fab')2].
abstract: OBJECTIVE
  To establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime.
  
  
  METHODS
  AntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF.
  
  
  RESULTS
  Around 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100.
  
  
  CONCLUSIONS
  The three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Fan
authors.forename: Yuan-Sheng
authors.initials: YS
authors.lastname: Xu
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Xiong
authors.forename: Chun-Zheng
authors.initials: CZ
authors.lastname: Yang
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D018951:Antigens, CD20 / Q000276:immunology*; D002845:Chromatography / Q000379:methods*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D018951:Antigens, CD20; D007140:Immunoglobulin Fab Fragments