Dendron-modified polystyrene microtiter plate: surface characterization with picoforce AFM and influence of spacing between immobilized amyloid beta proteins.
A polystyrene microtiter plate was coated with a molecular layer of a cone-shaped dendron as a means of providing proper spacing between immobilized biomolecules. For the coating preparation, di(ethylene glycol) vinyl ether was grafted onto the surface of the microtiter plate by a plasma process followed by self-assembly of a second-generation dendron (9-acid) or a third-generation dendron (27-acid). Contact angle analysis revealed a pronounced increase in the hydrophilicity upon plasma grafting, while the hydrophilicity reverted/decreased after dendron immobilization. For analysis by force-based atomic force microscopy (AFM), oligonucleotides were immobilized onto the AFM tip and the plate. The DNA-DNA interaction was observed at all spots examined, which implied that coating of the dendrons was uniform over the entire surface. The effectiveness for biomolecular assays of the spacing on dendron-modified microtiter plates was examined by carrying out an enzyme-linked immunosorbent assay (ELISA), where enhanced detection of different fragments of amyloid beta protein (A beta) was observed when compared with other conventional plates, such as untreated polystyrene or maleic anhydride activated plates. The positive influence of the mesospacing between biomolecules on the microtiter plates for this assay was confirmed.
Roy
Dhruvajyoti
D
Center for Integrated Molecular Systems, Departments of Chemistry and Materials Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Kwak
Ju-Won
JW
Maeng
Wan Joo
WJ
Kim
Hyungjun
H
Park
Joon Won
JW
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D016229:Amyloid beta-Peptides; D011137:Polystyrenes; D014867:Water; D004247:DNA