High-throughput screening platform for anticancer therapeutic drug cytotoxicity.
There are substances that kill cancer cells, but induce T cell proliferation, like thalidomide. To find more of these, a new anticancer drug screening strategy is vital. In this study we report the development of a differential cytotoxicity screening or evaluation platform using the CellTiter-Glo (Promega, Annandale, NSW, Australia) luminescent cell viability assay (ATP assay) and also the CellTiter 96 AQueous (Promega) one solution cell proliferation assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay]. The results suggested the platform consisting of the combination of the ATP assay be used for quantifying peripheral blood mononuclear cells, while the more economic MTS colorimetric assay is well suited to be used detecting cell viability of cancer cells. In addition, we found paclitaxel (Taxol, MP Biomedicals Australasia Pty Ltd., Seven Hills, NSW, Australia) to be a useful control for this routine screening methodology. Taxol exhibits the desirable in vitro feature of differential cytotoxicity that spares the immunological cells, when used at a concentration that will kill the majority of the cancer cell population.
Sekhon
Bhagwant Kaur
BK
Faculty of Pharmacy, The University of Sydney, Sydney, New South Wales, Australia.
Roubin
Rebecca Heidi
RH
Tan
Aaron
A
Chan
Wing Keung
WK
Sze
Daniel Man-Yuen
DM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D009000:Monocytes / Q000187:drug effects; D009101:Multiple Myeloma / Q000188:drug therapy / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology; D011429:Propolis / Q000494:pharmacology; D015203:Reproducibility of Results; D013778:Tetrazolium Salts; D013844:Thiazoles; D014343:Trypan Blue
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D013844:Thiazoles; C070380:3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; D000255:Adenosine Triphosphate; D011429:Propolis; D014343:Trypan Blue; D017239:Paclitaxel