title: The histological and clinical effects of 630 nanometer and 860 nanometer low-level laser on rabbits' ear punch holes.
abstract: Low-level laser therapy (LLLT) studies on the musculoskeletal and cartilage tissues of rabbits have reported conflicting results. We aimed to investigate the effects of 630 nm and 860 nm low-level laser on injured rabbit cartilage. After punching 5 mm holes in both ears of ten rabbits, we grouped the rabbits randomly. The punched holes of the laser-treated group were irradiated with 630 nm and 860 nm diode laser on days 3-5 and then every other day until day 20. In both laser and control groups, the hole diameters were measured weekly. Histological evaluation was carried out on day 30. The inter-group difference in hole diameters was not significant. Mann-Whitney U tests showed significant inter-group differences in histological variables related to chondrocyte production and organization, growth rate, granulation tissue and pseudocarcinomatosis. LLLT improved cartilage formation and reduced inflammation and formation of granulation tissue. More accurate results on its healing effects warrant studies with larger sample sizes.
authors.affiliation: ENT, Head & Neck Surgery Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Kamran
authors.initials: SK
authors.lastname: Kamrava
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farhadi
authors.forename: Farhad
authors.initials: F
authors.lastname: Rezvan
authors.forename: Davood
authors.initials: D
authors.lastname: Sharifi
authors.forename: Javad
authors.initials: J
authors.lastname: Ashrafihellan
authors.forename: Shervan
authors.initials: S
authors.lastname: Shoaee
authors.forename: Bijan
authors.initials: B
authors.lastname: Rezvan
mesh_terms: D000818:Animals; D019902:Chondrocytes / Q000473:pathology / Q000528:radiation effects; D004425:Ear Cartilage / Q000293:injuries* / Q000473:pathology / Q000528:radiation effects*; D006097:Granulation Tissue / Q000473:pathology; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D011817:Rabbits; D012038:Regeneration / Q000528:radiation effects; D014945:Wound Healing / Q000528:radiation effects