title: Human embryonic stem cells reveal recurrent genomic instability at 20q11.21.
abstract: By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5-4.6 Mb at 20q11.21, encompassing approximately 23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture.
authors.affiliation: INSERM/UEVE UMR-861, I-STEM, AFM, Institute for Stem cell Therapy and Exploration of Monogenic diseases, 5 rue Henri Desbruères, 91030 Evry cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lefort
authors.forename: Maxime
authors.initials: M
authors.lastname: Feyeux
authors.forename: Cécile
authors.initials: C
authors.lastname: Bas
authors.forename: Olivier
authors.initials: O
authors.lastname: Féraud
authors.forename: Annelise
authors.initials: A
authors.lastname: Bennaceur-Griscelli
authors.forename: Gerard
authors.initials: G
authors.lastname: Tachdjian
authors.forename: Marc
authors.initials: M
authors.lastname: Peschanski
authors.forename: Anselme L
authors.initials: AL
authors.lastname: Perrier
mesh_terms: D002460:Cell Line; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D053595:Embryonic Stem Cells / Q000166:cytology*; D005784:Gene Amplification; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D012008:Recurrence