title: Size- and distance-dependent nanoparticle surface-energy transfer (NSET) method for selective sensing of hepatitis C virus RNA.
abstract: We report size- and distance-dependent surface-energy transfer (SET) properties of gold nanoparticles for recognizing hepatitis C virus (HCV) RNA sequence sensitively and selectively (single-base mutations) in a homogeneous format. We have demonstrated that quenching efficiency increases by three orders of magnitude, as the particle size increases from 5 to 70 nm. Due to this extraordinarily high K(SV), nanoparticle SET (NSET) detection limit can be as low as 300 fM concentration of RNA, depending on the size of gold nanoparticle. We have shown that the distance-dependent quenching efficiency is highly dependent on the particle size and the distance at which the energy-transfer efficiency is 50 %, ranges all the way from 8 nm, which is very close to the accessible distance of conventional Förster resonance energy transfer (FRET), to about 40 nm by choosing gold nanoparticles of different diameters. Our result points out that dipole-to-metal-particle energy transfer and NSET models provide a better description of the distance dependence of the quenching efficiencies for 8 nm gold nanoparticle, but agreement is poor for 40 and 70 nm gold nanoparticles, for which the measured values were always larger than the predicted ones.
authors.affiliation: Department of Chemistry, Jackson State University, Jackson, MS, USA.
authors.forename: Jelani
authors.initials: J
authors.lastname: Griffin
authors.forename: Anant Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Dulal
authors.initials: D
authors.lastname: Senapati
authors.forename: Patsy
authors.initials: P
authors.lastname: Rhodes
authors.forename: Kanieshia
authors.initials: K
authors.lastname: Mitchell
authors.forename: Brianica
authors.initials: B
authors.lastname: Robinson
authors.forename: Eugene
authors.initials: E
authors.lastname: Yu
authors.forename: Paresh Chandra
authors.initials: PC
authors.lastname: Ray
mesh_terms: D001483:Base Sequence; D004735:Energy Transfer; D006046:Gold / Q000737:chemistry; D016174:Hepacivirus / Q000235:genetics*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010316:Particle Size; D020641:Polymorphism, Single Nucleotide; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D012367:RNA, Viral; D006046:Gold