title: An enzyme cocktail for efficient protoplast formation in Aspergillus niger.
abstract: Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia.
authors.affiliation: Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Charissa
authors.initials: C
authors.lastname: de Bekker
authors.forename: Ad
authors.initials: A
authors.lastname: Wiebenga
authors.forename: Guillermo
authors.initials: G
authors.lastname: Aguilar
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
mesh_terms: D000818:Animals; D001234:Aspergillus niger / Q000648:ultrastructure*; D002688:Chitinases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006372:Helix, Snails / Q000201:enzymology; D011523:Protoplasts; D013305:Streptomyces griseus / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology
chemical_list: D002688:Chitinases; D005966:Glucuronidase