Expression of enzymatically active L-DOPA decarboxylase in human peripheral leukocytes.
L-DOPA decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyses the decarboxylation of L-DOPA to dopamine. Dopamine (DA) has been found to be a regulating factor of the proliferation and differentiation of different leukocyte subtypes. In the present study, we report the expression of the gene that codes for the L-DOPA decarboxylase in human peripheral leukocytes and in T-lymphocytes, as well as the simultaneous detection of both neural and non-neural type DDC mRNA in the cellular components of this specialized connective tissue type. Furthermore, we have detected the neural type DDC transcript which lacks exon 3 and the alternative 37 kD alt-DDC protein isoform which lacks exons 10-15 but includes an alternative exon 10 in human peripheral leukocytes. Treatment of white blood cells with Triton X-114 resulted in the recovery of DDC in the detergent enriched and highly hydrophobic phases, suggesting association of DDC molecules with membranes in the studied cells. Enzymatic activity experiments revealed that DDC is active towards the decarboxylation of L-DOPA. The expression of enzymatically active DDC in human leukocytes could indicate a cross-talk between the nervous and the immune systems and raises new questions about the regulatory role of DDC in immune responses.
Kokkinou
Ioanna
I
Department of Biochemistry-Molecular Biology, University of Athens, Athens, Greece.
Nikolouzou
Eleftheria
E
Hatzimanolis
Alexandros
A
Fragoulis
Emmanuel G
EG
Vassilacopoulou
Dido
D
D017398:Alternative Splicing; D004296:Dopa Decarboxylase / Q000235:genetics / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D005091:Exons / Q000235:genetics; D006801:Humans; D007962:Leukocytes / Q000201:enzymology* / Q000276:immunology; D007980:Levodopa / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology* / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology
D020033:Protein Isoforms; D007980:Levodopa; D004296:Dopa Decarboxylase; D004298:Dopamine