title: The potential role of suppressors of cytokine signaling in the attenuation of inflammatory reaction and alveolar bone loss associated with apical periodontitis.
abstract: Inflammatory cytokines contribute to periapical tissue destruction. Their activity is potentially regulated by suppressors of cytokine signaling (SOCS), which downregulate signal transduction as part of an inhibitory feedback loop. We investigated the expression of the cytokines tumor necrosis factor alpha (TNF-alpha); interleukin (IL)-10 and RANKL; and SOCS-1, -2, and -3 by real-time polymerase chain reaction in 57 periapical granulomas and 38 healthy periapical tissues. Periapical granulomas exhibited significantly higher SOCS-1, -2, and -3, TNF-alpha, IL-10, and RANKL messenger RNA levels when compared with healthy controls. Significant positive correlations were found between SOCS1 and IL-10 and between SOCS3 and IL-10. Significant inverse correlations were observed between SOCS1 and TNF-alpha, SOCS1 and RANKL, and SOCS3 and TNF-alpha. Increased SOCS-1, -2, and -3 messenger RNA levels in periapical granulomas may be related to the downregulation of inflammatory cytokines in these lesions; therefore, SOCS molecules may play a role in the dynamics of periapical granulomas development.
authors.affiliation: Department of Biological Sciences, Bauru Dental School, University of São Paulo Bauru, SP, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Menezes
authors.forename: Thiago Pompermaier
authors.initials: TP
authors.lastname: Garlet
authors.forename: Ana Paula Fávaro
authors.initials: AP
authors.lastname: Trombone
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Repeke
authors.forename: Ariadne
authors.initials: A
authors.lastname: Letra
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
authors.forename: Gustavo Pompermaier
authors.initials: GP
authors.lastname: Garlet
mesh_terms: D000293:Adolescent; D000328:Adult; D016301:Alveolar Bone Loss / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010484:Periapical Granuloma / Q000276:immunology; D010485:Periapical Periodontitis / Q000276:immunology*; D010486:Periapical Tissue / Q000276:immunology; D053245:RANK Ligand / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000276:immunology; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000032:analysis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D055815:Young Adult
chemical_list: D053245:RANK Ligand; D012333:RNA, Messenger; C108944:SOCS1 protein, human; C106643:SOCS2 protein, human; C106644:SOCS3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10