title: Characteristics of sulfobacin A from a soil isolate Chryseobacterium gleum.
abstract: A nonmotile, nonspore-forming, Gram-negative, aerobic, small rod-shaped bacterium, isolated from soil, was identified as Chryseobacterium gleum on the basis of 16S rRNA gene sequence analysis. It was observed to grow luxuriously at pH 9 and tolerate highly alkaline environment up to pH 12. Orange red color was a peculiar character of these cells which on purification obtained 60-80 mg/l and found to be sphingosine type of sulfonolipid "sulfobacin A" on the basis of infrared, nuclear magnetic resonance, and mass spectral data. Inhibition of sulfobacin A synthesis by incorporation of L: -cycloserine in culture growth medium suggested presence of serine palmitoyl transferase which is one of the important enzymes involved in its biosynthesis. Sulfobacin A from C. gleum LMG P-22264 exhibited cytotoxicity against four cell lines tested. Maximum activity against human mammary adenocarcinoma cells was indicative of its potential as an anticancer agent.
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, India.
authors.forename: Priti N
authors.initials: PN
authors.lastname: Chaudhari
authors.forename: Kishor S
authors.initials: KS
authors.lastname: Wani
authors.forename: Bhushan Liladhar
authors.initials: BL
authors.lastname: Chaudhari
authors.forename: Sudhir B
authors.initials: SB
authors.lastname: Chincholkar
mesh_terms: D017738:Alkanesulfonic Acids / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045247:Chryseobacterium / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D003523:Cycloserine / Q000494:pharmacology; D006801:Humans; D010802:Phylogeny; D010858:Pigmentation; D011090:Polyenes / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013057:Spectrum Analysis; D044366:Transition Temperature
chemical_list: D017738:Alkanesulfonic Acids; D016588:Anticarcinogenic Agents; D011090:Polyenes; D012336:RNA, Ribosomal, 16S; C096615:sulfobacin A; C017313:flexirubins; D003523:Cycloserine