Cytostatic and cytotoxic properties of chimeric peptides containing cyclin-inhibiting fragments.
Properties of chimeric peptides containing cell-penetrating sequences and pl6INK4a and E2F fragments were studied in vitro on immurtal cultures of human cells. Both sequences exhibit cytostatic activity. The peptide containing fragment p16INK4a inhibited proliferation during the G0/G1 stage of the cell cycle, while sequence E2F suppressed proliferation in S phase. Both sequences exhibit cytotoxic properties and can induce apoptosis.
Kulinich
T M
TM
Russian Research Center of Roentgenology and Radiology, Russian Medical Technologies, Moscow. sobral@mail.ru
Kharchenko
V P
VP
Filyasova
E I
EI
Shishkin
A M
AM
Bozhenko
V K
VK
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000037:antagonists & inhibitors*; D054697:Cytostatic Agents / Q000378:metabolism*; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D054697:Cytostatic Agents; D050687:E2F1 Transcription Factor; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins