Anti-inflammatory activity of Schizonepeta tenuifolia through the inhibition of MAPK phosphorylation in mouse peritoneal macrophages.
Schizonepeta tenuifolia (ST) is a well-known herb to treat the cold and its associated headache. However, the anti-inflammatory mechanism of ST in mouse peritoneal macrophages is not clear. In this study, we demonstrated that ST inhibited lipopolysaccaride (LPS)-induced tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production. The maximal inhibition rate of TNF-alpha and IL-6 production by ST (2 mg/ml) was 48.01 +/- 2.8% and 56.45 +/- 2.8%, respectively. During the inflammatory process, cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) were increased in mouse peritoneal macrophages. However, treated with ST decreased the protein level of COX-2 and iNOS, as well as the production of PGE(2) and NO in LPS-stimulated mouse peritoneal macrophages. In addition, ST inhibited the phosphorylation of MAPK. Taken together, the results of this study suggest an important molecular mechanism by which ST reduces inflammation, which may explain its beneficial effect in the regulation of inflammatory reactions.
Kim
Su-Jin
SJ
College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
Kim
Jung-Sun
JS
Choi
In-Young
IY
Kim
Dong-Hyun
DH
Kim
Min-Cheol
MC
An
Hyo-Jin
HJ
Na
Ho-Jeong
HJ
Kim
Na-Hyung
NH
Moon
Phil-Dong
PD
Myung
Noh-Yil
NY
Lee
Ju-Young
JY
Jeong
Hyun-Ja
HJ
Um
Jae-Young
JY
Shin
Tae-Yong
TY
Kim
Hyung-Min
HM
Hong
Seung-Heon
SH
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000276:immunology; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D015850:Interleukin-6 / Q000276:immunology; D019686:Lamiaceae / Q000737:chemistry*; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020929:Mitogen-Activated Protein Kinase Kinases / Q000276:immunology / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D020929:Mitogen-Activated Protein Kinase Kinases