title: [RNA interference of hnRNP B1 gene in human A549 lung cells].
abstract: OBJECTIVE
  To test the effect of RNA interference of hnRNP Bx gene in human lung cell line A549.
  
  
  METHODS
  The RNAs of cell line A549 were interfered by the RNA interference technique. The hnRNP B1 mRNAs were detected by fluorescence quantity RT-PCR. The proteins of hnRNP B1 were detected by Western blot technique. The growth rate of the cells was analysed by MTT.
  
  
  RESULTS
  The growth of the cells was suppressed significantly. The mRNA and protein expressions of hnRNP B1 were inhibited.
  
  
  CONCLUSIONS
  The expression of hnRNP B1 can be inhibited by RNA interference. RNA interference could become a possible clinical therapy for lung cancers.
authors.affiliation: Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Pu
authors.forename: Tao-you
authors.initials: TY
authors.lastname: Zhou
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Feng-ming
authors.initials: FM
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Han
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C439768:hnRNP A2