title: [Establishing the method of collagen-gel droplet embedded culture drug sensitivity test with rat tail collagen].
abstract: OBJECTIVE
  To explore the clinical application of collagen-gel droplet embedded-culture drug sensitivity test (CD-DST) in the malignant tumors.
  
  
  METHODS
  CD-DST was established with rat tail collagen. Three pancreatic cancer cell lines, surgical resection specimens including 15 cases of pancreatic cancer and 10 cases of gastrointestinal cancer were examined using CD-DST.
  
  
  RESULTS
  The overall achievement ratio of CD-DST for clinical tumor specimens was 80% (20/25). In vitro chemosensitivities of pancreatic carcinoma cells to 5-FU, gemcitabine and oxaliplatin were lower than those of gastrointestinal carcinoma.
  
  
  CONCLUSIONS
  CD-DST with rat tail collagen is a valuable chemosensitivity testing method for malignant tumors. It can be used to realize individualized anticancer chemotherapy.
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Hu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Quan
authors.initials: Q
authors.lastname: Liao
authors.forename: Tai-Ping
authors.initials: TP
authors.lastname: Zhang
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.forename: Yu-Pei
authors.initials: YP
authors.lastname: Zhao
mesh_terms: D000818:Animals; D003094:Collagen; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D005782:Gels; D006801:Humans; D008297:Male; D051381:Rats; D013623:Tail / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D005782:Gels; D003094:Collagen