[The effect of NGF on hematopoiesis of granulocyte-macrophage in normal and radiation, chemistry injury mice].
OBJECTIVE
To explore the effect of nerve growth factor (NGF) on hematopoiesis of granulocyte-macrophage and to elucidate the underlying mechanism.


METHODS
Using flow cytometry, colony forming assay, blood cell count, fluorescent real-time quantitation PCR, and enzyme-linked immunosorbent assay (ELISA), changes in the bone marrow cells (BMCs) proliferation cycle, CFU-GM counts, the peripheral white blood cell (WBC)counts, BMC GM-CSF mRNA quantities, and serum GM-CSF, IL-3 concentrations were observed after NGF was injected intramuscularly into the thigh of normal and combined 60Co gamma ray irradiated and cyclophosphamide peritoneal cavity injected(combined radiation and chemistry injury mice, 60Co-Cy injury) mice. The effect of NGF on CFU-GM formation in vitro cooperation with exogenous GM-CSF or alone was also observed.


RESULTS
An injection of NGF [7.5 microg/(kg x d) or 10 microg/(kg x d)] lasted 7 days, the S+G2/M proportions and the CFU-GM counts of BMCs rose greater than injection of physiological saline (PS) in normal and 60Co-Cy injury mice; the serum GM-CSF,IL-3 concentration in 60Co-Cy injury mice rose obviously. In vitro, NGF stimulated a dose-dependent increase of CFU-GM colonies formation in the semisolid culture system with exogenous rmGM-CSF.


CONCLUSIONS
NGF cooperates with hematopoietic stimulating factors in culture system to promote the CFU-GM colonies in normal and 60Co-Cy injury mice in vitro. NGF accelerates the BMCs into mitosis, the hematopoietic stem cells differentiation into granulocyte-macrophage hematopoietic cells, the CFU-GM formation and promotes the white blood cells count in normal and 60Co-Cy injury mice in vivo, also NGF stimulates the secreting of GM-CSF and IL-3 in 60Co-Cy injury mice,this may be one of the reasons why NGF stimulate the granulocyte-macrophage hematopoiesis repair after combined radiation and chemistry injury.
Huang
Xiao-qin
XQ
Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhu
Bi-de
BD
Jiang-Yang-ze-ren
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000473:pathology; D003520:Cyclophosphamide / Q000009:adverse effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects*; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000494:pharmacology*; D011832:Radiation Injuries / Q000097:blood*
D009414:Nerve Growth Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003520:Cyclophosphamide