title: Anti-inflammatory activity of Schizonepeta tenuifolia through the inhibition of MAPK phosphorylation in mouse peritoneal macrophages.
abstract: Schizonepeta tenuifolia (ST) is a well-known herb to treat the cold and its associated headache. However, the anti-inflammatory mechanism of ST in mouse peritoneal macrophages is not clear. In this study, we demonstrated that ST inhibited lipopolysaccaride (LPS)-induced tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production. The maximal inhibition rate of TNF-alpha and IL-6 production by ST (2 mg/ml) was 48.01 +/- 2.8% and 56.45 +/- 2.8%, respectively. During the inflammatory process, cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) were increased in mouse peritoneal macrophages. However, treated with ST decreased the protein level of COX-2 and iNOS, as well as the production of PGE(2) and NO in LPS-stimulated mouse peritoneal macrophages. In addition, ST inhibited the phosphorylation of MAPK. Taken together, the results of this study suggest an important molecular mechanism by which ST reduces inflammation, which may explain its beneficial effect in the regulation of inflammatory reactions.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kim
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: An
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Na-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Noh-Yil
authors.initials: NY
authors.lastname: Myung
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000276:immunology; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D015850:Interleukin-6 / Q000276:immunology; D019686:Lamiaceae / Q000737:chemistry*; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020929:Mitogen-Activated Protein Kinase Kinases / Q000276:immunology / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D020929:Mitogen-Activated Protein Kinase Kinases