title: Bioactivity-guided isolation of cytotoxic constituents of Brucea javanica collected in Vietnam.
abstract: Five new triterpenoids (1-5), together with two known quassinoids, bruceantin (6) and bruceine A (7), and a known flavonolignan, (-)-hydnocarpin (8), were isolated from the chloroform-soluble subfraction of a methanol extract of the combined twigs, leaves, and inflorescence of Brucea javanica collected in Vietnam. The structures of the new compounds 1-5 were established on the basis of spectroscopic methods. All isolates were evaluated for cytotoxicity against a small panel of human cancer cell lines. Quassinoids 6 and 7 were found to be highly active against these cell lines. (-)-Hydnocarpin (8) showed a potentiating effect when combined with both 6 and 7, during cytotoxicity testing using the MCF-7 human breast cancer cell line.
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, 500 West 12th Avenue, Columbus, OH 43210, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
authors.forename: Hee-Byung
authors.initials: HB
authors.lastname: Chai
authors.forename: Tran Ngoc
authors.initials: TN
authors.lastname: Ninh
authors.forename: Djaja Djendoel
authors.initials: DD
authors.lastname: Soejarto
authors.forename: A Douglas
authors.initials: AD
authors.lastname: Kinghorn
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D032269:Brucea / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D014744:Vietnam
chemical_list: D000972:Antineoplastic Agents, Phytogenic