Rapid determination of ethanol in fermentation liquor by full evaporation headspace gas chromatography.
This paper reports a full evaporation (FE) headspace gas chromatographic (GC) method for rapid determination of ethanol in fermentation liquor. The data show that ethanol in the fermentation liquor was transferred to the vapor phase (headspace) almost completely within 3 min at a temperature of 105 degrees C when a very small volume (< 50 microL) of sample was directly added to a sealed headspace sample vial (20 mL). The ethanol in the vapor phase was then measured by headspace GC using a flame ionization detector. The results show that the present method has an excellent measurement precision (RSD=1.62%) and accuracy (recovery=98.1 (+/-1.76%)) for the ethanol quantification in fermentation liquors. The method requires no sample pretreatment and is very simple and rapid.
Li
Hailong
H
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, Guangdong 510640, China.
Chai
Xin-Sheng
XS
Deng
Yulin
Y
Zhan
Huaiyu
H
Fu
Shiyu
S
D000434:Alcoholic Beverages / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D000431:Ethanol / Q000032:analysis*; D005285:Fermentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014835:Volatilization
D000431:Ethanol