[Modified Ziehl-Neelsen and modified Safranin staining for diagnosing Cyclospora cayetanensis].
OBJECTIVE
Comparing modified Ziehl-Neelsen (ZNm) and modified Safranin (Sm) staining for detecting C. cayetanensis oocysts in stool samples.


METHODS
The sample to be analysed consisted of 100 stool samples which had been previously evaluated by direct microscopic examination and concentration. Each sample was then ZNm and Sm stained. Microscope examination was used as gold standard for comparing both techniques by statistical analysis.


RESULTS
There was 95% sensitivity and 90% specificity for ZNm and 98% sensitivity and 100% specificity for Sm. The Kappa index was 0.93, signifying a very good degree of agreement between the two techniques.


CONCLUSIONS
The use of either of the two stains for diagnosing C. cayetanensis can be recommended due to the high sensitivity and specificity for Zm and Sm found in this study and to the high degree of agreement between them.
Galvan-Díaz
Ana L
AL
Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia. agalvandiaz@yahooo.com
Herrera-Jaramillo
Viviana
V
Santos-Rodriguez
Zila M
ZM
Delgado-Naranjo
Marcela
M
D000818:Animals; D004396:Coloring Agents; D021744:Cyclospora / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D010619:Phenazines; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; D010619:Phenazines; C009195:safranine T