title: A bifunctional archaeal protein that is a component of 30S ribosomal subunits and interacts with C/D box small RNAs.
abstract: We have identified a novel archaeal protein that apparently plays two distinct roles in ribosome metabolism. It is a polypeptide of about 18 kDa (termed Rbp18) that binds free cytosolic C/D box sRNAs in vivo and in vitro and behaves as a structural ribosomal protein, specifically a component of the 30S ribosomal subunit. As Rbp18 is selectively present in Crenarcheota and highly thermophilic Euryarchaeota, we propose that it serves to protect C/D box sRNAs from degradation and perhaps to stabilize thermophilic 30S subunits.
authors.affiliation: Dpt. Biotecnologie Cellulari ed Ematologia, Università di Roma La Sapienza (Policlinico) Viale Regina Elena 324, 00161 Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ciammaruconi
authors.forename: Stefania
authors.initials: S
authors.lastname: Gorini
authors.forename: Paola
authors.initials: P
authors.lastname: Londei
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D019642:RNA, Archaeal / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D054749:Ribosome Subunits, Small, Archaeal / Q000737:chemistry* / Q000378:metabolism; D048229:Sulfolobus solfataricus / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D019642:RNA, Archaeal; D016601:RNA-Binding Proteins