title: Culture versus polymerase chain reaction for the etiologic diagnosis of community-acquired pneumonia in antibiotic-pretreated pediatric patients.
abstract: Tracheal aspirates were collected from 176 hospitalized antibiotic-pretreated children with community-acquired pneumonia. Haemophilus influenzae and Streptococcus pneumoniae were detected by both culture and target-enriched multiplex (TEM)-PCR whereas Mycoplasma pneumoniae and Chlamydia pneumoniae were detected by TEM-PCR only. TEM-PCR detected more S. pneumoniae (32 vs. 7) and H. influenzae (29 vs. 23) than did culture. TEM-PCR detected an additional 26 M. pneumoniae and 1 C. pneumoniae. TEM-PCR significantly enhances the pathogen-specific diagnosis of CAP in children.
authors.affiliation: Shenzhen Children's Hospital, China Medical University, Shenzhen, China.
authors.forename: Jikui
authors.initials: J
authors.lastname: Deng
authors.forename: Yuejie
authors.initials: Y
authors.lastname: Zheng
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Zhao
authors.forename: Peter F
authors.initials: PF
authors.lastname: Wright
authors.forename: Charles W
authors.initials: CW
authors.lastname: Stratton
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D019072:Antibiotic Prophylaxis; D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000188:drug therapy / Q000382:microbiology* / Q000517:prevention & control; D005260:Female; D006193:Haemophilus influenzae / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008297:Male; D009177:Mycoplasma pneumoniae / Q000145:classification / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000188:drug therapy / Q000382:microbiology* / Q000517:prevention & control; D016133:Polymerase Chain Reaction / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000145:classification / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents