title: Dendritic cells modulate lung response to Pseudomonas aeruginosa in a murine model of sepsis-induced immune dysfunction.
abstract: Host infection by pathogens triggers an innate immune response leading to a systemic inflammatory response, often followed by an immune dysfunction which can favor the emergence of secondary infections. Dendritic cells (DCs) link innate and adaptive immunity and may be centrally involved in the regulation of sepsis-induced immune dysfunction. We assessed the contribution of DCs to lung defense in a murine model of sublethal polymicrobial sepsis (cecal ligature and puncture, CLP). In this model, bone marrow-derived DCs (BMDCs) retained an immature phenotype, associated with decreased capacity of IL-12p70 release and impaired priming of T cell lymphocytes. Eight days after CLP surgery, we induced a secondary pulmonary infection through intratracheal instillation of 5 x 10(6) CFUs of Pseudomonas aeruginosa. Whereas all sham-operated mice survived, 80% of post-CLP mice died after secondary pneumonia. Post-CLP mice exhibited marked lung damage with early recruitment of neutrophils, cytokine imbalance with decreased IL-12p70 production, and increased IL-10 release, but no defective bacterial lung clearance, while systemic bacterial dissemination was almost constant. Concomitant intrapulmonary administration of exogenous BMDCs into post-CLP mice challenged with P. aeruginosa dramatically improved survival. BMDCs did not improve bacterial lung clearance, but delayed neutrophil recruitment, strongly attenuated the early peak of TNF-alpha and restored an adequate Il-12p70/IL-10 balance in post-CLP mice. Thus, adoptive transfer of BMDCs reversed sepsis-induced immune dysfunction in a relevant model of secondary P. aeruginosa pneumonia. Unexpectedly, the mechanism of action of BMDCs did not involve enhanced antibacterial activity, but occurred by dampening the pulmonary inflammatory response.
authors.affiliation: Department of Cellular Biology, Centre National de la Recherche Scientifique, Cochin Institute, University Paris-Descartes, Paris, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Pène
authors.forename: Benjamin
authors.initials: B
authors.lastname: Zuber
authors.forename: Emilie
authors.initials: E
authors.lastname: Courtine
authors.forename: Christophe
authors.initials: C
authors.lastname: Rousseau
authors.forename: Fatah
authors.initials: F
authors.lastname: Ouaaz
authors.forename: Julie
authors.initials: J
authors.lastname: Toubiana
authors.forename: Asmaa
authors.initials: A
authors.lastname: Tazi
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Mira
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Chiche
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000473:pathology; D002432:Cecum; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology* / Q000637:transplantation; D004195:Disease Models, Animal; D005260:Female; D008026:Ligation; D008168:Lung / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018410:Pneumonia, Bacterial / Q000276:immunology* / Q000382:microbiology* / Q000401:mortality; D011552:Pseudomonas Infections / Q000276:immunology* / Q000382:microbiology* / Q000401:mortality; D011677:Punctures