Glass transition behavior of the vitrification solutions containing propanediol, dimethyl sulfoxide and polyvinyl alcohol.
Knowledge of the glass transition behavior of vitrification solutions is important for research and planning of the cryopreservation of biological materials by vitrification. This brief communication shows the analysis for the glass transition and glass stability of the multi-component vitrification solutions containing propanediol (PE), dimethyl sulfoxide (Me2SO) and polyvinyl alcohol (PVA) by using differential scanning calorimetry (DSC) during the cooling and subsequent warming between 25 and -150 degrees C. The glass formation of the solutions was enhanced by introduction of PVA. Partial glass formed during cooling and the fractions of free water in the partial glass matrix increased with the increasing of PVA concentration, which caused slight decline of glass transition temperature, T(g). Exothermic peaks of devitrification were delayed and broadened, which may result from the inhibition of ice nucleation or recrystallization of PVA.
Wang
Hai-Yan
HY
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Xingangxi Road, No. 135, Guangzhou, Guangdong 510275, China.
Lu
Shu-Shen
SS
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Xingangxi Road, No. 135, Guangzhou, Guangdong 510275, China. Electronic address: lvshsh@mail.sysu.edu.cn.
Lun
Zhao-Rong
ZR
School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
D002152:Calorimetry, Differential Scanning; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D044367:Phase Transition; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
D003451:Cryoprotective Agents; D011409:Propylene Glycols; D011142:Polyvinyl Alcohol; D004121:Dimethyl Sulfoxide