title: The use of elastin immunostain improves the evaluation of melanomas associated with nevi.
abstract: BACKGROUND
  Twenty to 30% of malignant melanomas are associated with melanocytic nevi; however, sometimes it is difficult to distinguish the melanoma from the nevus by routine histology. We have previously described distinctive patterns of elastic fibers in nevi and in melanomas.
  
  
  METHODS
  We analyzed elastic fiber patterns using elastin immunostain and elastic van Gieson (EVG) stain in 30 cases of invasive melanomas associated with nevi, 12 control melanocytic nevi and 14 control invasive melanomas.
  
  
  RESULTS
  Elastin immunostain was superior to EVG in showing the elastic fiber patterns. In nevi, the elastic fibers were preserved between nests and often around individual melanocytes. In contrast, melanomas had markedly decreased elastic fibers in the stroma and within the nests of melanocytes. The melanoma pushed down the pre-existing thin elastic fibers of the papillary dermis, forming a compressed layer at its base, which separated the melanoma from the nevus. On sun-damaged skin, the solar elastosis had similar elastin and EVG patterns. In three cases with dense inflammation, the layer of elastic fibers between melanoma and nevus was still present but less evident.
  
  
  CONCLUSIONS
  The distinctive patterns of elastic fibers, best shown by the elastin immunostain, were helpful in evaluating melanomas associated with melanocytic nevi.
authors.affiliation: Dermatopathology Section, Department of Dermatology, New York University School of Medicine, New York, NY 10016, USA. hideko.kamino@nyumc.org
authors.forename: Hideko
authors.initials: H
authors.lastname: Kamino
authors.forename: Sam
authors.initials: S
authors.lastname: Tam
authors.forename: Beatriz
authors.initials: B
authors.lastname: Tapia
authors.forename: Sonia
authors.initials: S
authors.lastname: Toussaint
mesh_terms: D004549:Elastin / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009506:Nevus / Q000378:metabolism* / Q000473:pathology*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology*; D013472:Sunlight / Q000009:adverse effects
chemical_list: D004549:Elastin