Peroxisome proliferator-activated receptor gamma is constitutively activated in yeast.
A rapid, simple in vitro test system for high-throughput screening of peroxisome proliferator-activated receptor (PPAR) gamma agonists would be of interest for testing new antidiabetic drugs, alternative medicine, or environmental samples. A yeast two-hybrid assay based on the ligand-dependent recruitment of the coactivator CBP (CREB-binding protein) was constructed. In this system PPARgamma was constitutively activated and the signal was not further increased significantly by adding agonists. In yeast we identified oleic acid as a putative endogenous ligand. Furthermore yeasts seem to lack regulatory mechanisms present in mammalian cells. Mammalian systems are an alternative for screening PPARgamma agonists.
Mueller
Monika
M
Christian Doppler Laboratory for Receptor Biotechnology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Jungbauer
Alois
A
D050882:CREB-Binding Protein / Q000378:metabolism; D055808:Drug Discovery / Q000379:methods*; D047495:PPAR gamma / Q000819:agonists*; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000737:chemistry*
D047495:PPAR gamma; D050882:CREB-Binding Protein