The effect of vitamin E on basic fibroblast growth factor level in human fibroblast cell culture.
Basic fibroblast growth factor (bFGF) is angiogenic and effective in down-regulating excess collagen production. The aim of this study is to evaluate the effectiveness of vitamin E (Tocotrienol Rich Fraction) in altering the level of bFGF, a cytokine involved in the scar formation process. In this model, normal human fibroblasts were treated with various concentrations of vitamin E at different time frames. The levels of bFGF were determined by Enzyme-Linked Immunosorbant Assay (ELISA). This study demonstrated that Tocotrienol Rich Fraction (TRF) stimulated bFGF production by fibroblast and postulate that vitamin E may decrease aberrant scar formation.
Rashid
S A Harun Nor
SA
Reconstructive Sciences Unit, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan.
Halim
A S
AS
Muhammad
N A
NA
D018929:Cell Culture Techniques; D002470:Cell Survival; D016207:Cytokines; D004797:Enzyme-Linked Immunosorbent Assay; D005346:Fibroblast Growth Factors / Q000096:biosynthesis / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008954:Models, Biological; D014810:Vitamin E / Q000494:pharmacology*
D016207:Cytokines; D014810:Vitamin E; D005346:Fibroblast Growth Factors