title: [Rescue of chimeric rabies virus expressing green fluorescent protein].
abstract: Green fluorescent protein (GFP) gene was inserted into the pseudogene (psi) region of genome of rabies virus rHep-Flury strain, and a recombinant rabies virus carrying GFP, designated as HEP-GFP, was rescued by reverse genetics system. It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line. The research indicated that the pseudogene (psi) region in the genome of rHEP-Flury strain, as an independent functional unit in the process of virus assembly, could independently carry and express exogenous genes.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Ai
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Guo
authors.forename: Jing-Chen
authors.initials: JC
authors.lastname: Sun
authors.forename: Hong-Ru
authors.initials: HR
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D049452:Green Fluorescent Proteins / Q000235:genetics*; D011820:Rabies virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins