title: Structural stability of Sclerotium rolfsii ATCC 201126 beta-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach.
abstract: OBJECTIVE
  Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences.
  
  
  RESULTS
  Fermenter-scale production led to productivity (P(r)) and yield (Y(P/C)) values higher at 48 h (P(r) = 0.542 g l(-1) h(-1); Y(P/C) = 0.74) than at 72 h (P(r) = 0.336 g l(-1) h(-1); Y(P/C) = 0.50). Both EPSs were neutral glucose-homopolysaccharides with a beta-(1,3)-glycosidic backbone and single beta-(1,6)-glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm(-1) confirmed beta-glycosidic linkages, while gentiobiose released by beta-(1,3)-glucanases confirmed single beta-1,6-glycosidic branching for both EPSs.
  
  
  CONCLUSIONS
  The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour.
  
  
  CONCLUSIONS
  Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.
authors.affiliation: PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Tucumán, Argentina. jifarina@yahoo.com
authors.forename: J I
authors.initials: JI
authors.lastname: Fariña
authors.forename: S C
authors.initials: SC
authors.lastname: Viñarta
authors.forename: M
authors.initials: M
authors.lastname: Cattaneo
authors.forename: L I C
authors.initials: LI
authors.lastname: Figueroa
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004355:Drug Stability; D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry; D010504:Periodic Acid / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D047071:beta-Glucans
chemical_list: D003470:Culture Media; D005936:Glucans; D047071:beta-Glucans; D010504:Periodic Acid; C045938:scleroglucan; C009288:metaperiodate