[Orthogonal experiments for optimizing the formulation and preparation conditions of temozolomide solid lipid nanoparticles].
TMZ-SLN were prepared by emulsification-low temperature solidification method with stearic acid. The formulation and the preparation conditions were optimized by orthogonal experiments using entrapment efficiency as the evaluation index. The morphology was detected by transmission electron microscope. The Zeta potentials and the particle size distribution were evaluated by Laser Doppler Anemometry. The entrapment efficiencies and the drug release characteristics in vitro were assessed. The result showed that TMZ-SLN were concinnous and spherical in shape. The mean diameter (d(av) ) was 65.0 +/- 6.2 nm and the Zeta potential was -37.2 mV. The average entrapment efficiency was 58.9% +/- 1.21 %. The drug release behavior in vitro conformed to Higuchi Equation. The formation of a new material phase was testified by analysis of differential scanning calorimetry.
Dou
Mingjin
M
College of Pharmacy, Shandong University, Ji'nan 250012, China.
Huang
Guihua
G
Xi
Yanwei
Y
Zhang
Na
N
D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000737:chemistry; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013229:Stearic Acids / Q000737:chemistry*; D000077204:Temozolomide
D018906:Antineoplastic Agents, Alkylating; D004337:Drug Carriers; D008055:Lipids; D013229:Stearic Acids; C031183:stearic acid; D003606:Dacarbazine; D000077204:Temozolomide