title: Chlamydia infection in patients with and without cervical intra-epithelial lesions tested by real-time PCR vs. direct immunofluorescence.
abstract: This study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife. We conducted a transversal study involving 35 women with SIL and 35 without SIL attended at Ambulatório Especializado da Mulher, Recife, Brazil. They were tested for Chlamydia trachomatis using two techniques, direct IMF or real time PCR. The rates of Chlamydia trachomatis detection were compared and the association with intra-epithelial cervical lesions was determined using the chi-square test at a 5% level of significance. Concordance between the tests was evaluated using kappa. The global prevalence of Chlamydia infection was 47.1% by direct IMF and 58.6% by real time PCR. A significant association was observed between Chlamydia diagnosis and presence of intra-epithelial cervical lesions, with about 80% positive results by direct IMF and 77.1% by real time PCR. However, the detected rate of infection with Chlamydia trachomatis was significantly greater in patients without intra-epithelial cervical lesions tested by real time PCR (40%) when compared to direct IMF (14.3%). The concordance between the tests was weak, with a kappa coefficient of 0.4. Both real time PCR and direct IMF detected elevated rates of Chlamydia infection in patients with intra-epithelial cervical lesions (80%) but the tests were discordant when patients without cervical lesions were tested, possibly because sensitivity of real time PCR is greater.
authors.affiliation: Prof. Fernando Figueira Institute (IMIP), Recife, PE, Brazil. melamorim@uol.com.br
authors.forename: Micheline de Lucena
authors.initials: Mde L
authors.lastname: Oliveira
authors.forename: Melania Maria Ramos de
authors.initials: MM
authors.lastname: Amorim
authors.forename: Paulo Roberto Eleutério de
authors.initials: PR
authors.lastname: Souza
authors.forename: Lúcia Cristina Bezerra de
authors.initials: LC
authors.lastname: Albuquerque
authors.forename: Lucas André Cavalcanti
authors.initials: LA
authors.lastname: Brandão
authors.forename: Rafael Lima
authors.initials: RL
authors.lastname: Guimarães
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D003430:Cross-Sectional Studies; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D002577:Uterine Cervical Diseases / Q000382:microbiology*