Antioxidants alleviate electric field-induced effects on lung tissue based on assays of heme oxygenase-1, protein carbonyl content, malondialdehyde, nitric oxide, and hydroxyproline.
In order to test whether antioxidants have beneficiary effects on electric field induced damage, we determined the pulmonary levels of heme oxygenase-1 (HO-1), protein carbonyl content (PCO), malondialdehyde (MDA), nitric oxide (NO) and hydroxyproline (HP) under extremely low frequency (ELF) electric (E) field exposure (50 Hz, 12 kV/m, 7 days/for 8 h/day). While PCO levels significantly increased (p<0.05), insignificant changes (p>0.05) were observed in HO-1, MDA, NO and HP levels for electric field exposure groups compared to the control group. We have not observed any significant change in these parameters on the electric field group compared to the group where NAC and EGCG were separately applied along with electric field. However, during our previous studies, we have concluded that NAC and EGCG are potent antioxidants and we believe that new studies should be established by way of setting up different experimental conditions.
Güler
Göknur
G
Department of Biophysics, Medical Faculty & Gazi Non-Ionizing Radiation Protection Center (GNRK), Gazi University, Besevler, 06500, Ankara, Turkey. gozturk@gazi.edu.tr
Türközer
Zerrin
Z
Ozgur
Elcin
E
Seyhan
Nesrin
N
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D044822:Biodiversity; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006168:Guinea Pigs; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008171:Lung Diseases / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D050050:Protein Carbonylation; D011897:Random Allocation
D000975:Antioxidants; D009569:Nitric Oxide; D008315:Malondialdehyde; D002392:Catechin; C045651:epigallocatechin gallate; D051547:Heme Oxygenase-1; D006909:Hydroxyproline; D000111:Acetylcysteine