title: Pseudomonas aeruginosa: mannose sensitive hemagglutinin inhibits the growth of human hepatocarcinoma cells via mannose-mediated apoptosis.
abstract: A vaccine derived from the outer membrane proteins of the Gram-negative bacterium Pseudomonas aeruginosa has been shown to have immune modulatory properties. An inactivated mutant strain of P. aeruginosa with mannose sensitive hemagglutinin fimbria (PA-MSHA) has been used for adjuvant therapy for malignant cancer. In this study, the growth of human hepatocellular carcinoma Hep G2 and BEL-7402 cells is inhibited by PA-MSHA, but not by mannose-cleaved PA-MSHA. PA-MSHA-treated cells arrested in the S phase of the cell cycle and underwent apoptosis. We hypothesize that apoptosis induced by treatment of Hep G2 and BEL-7402 cells with PA-MSHA is mediated by the mannose residues of PA-MSHA and is propagated through the extrinsic apoptosis pathway directly through caspase-8. These data provide mechanistic details for the potential application of PA-MSHA-based treatment of hepatocellular carcinoma.
authors.affiliation: Department of Intervention, The First Affiliated Clinic College of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Cao
authors.forename: Lijun
authors.initials: L
authors.lastname: Shi
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jinghua
authors.initials: J
authors.lastname: Wang
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Guangyou
authors.initials: G
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Lili
authors.initials: L
authors.lastname: Mu
authors.forename: Mingfei
authors.initials: M
authors.lastname: Yang
authors.forename: Hulun
authors.initials: H
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001428:Bacterial Vaccines / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology; D002453:Cell Cycle; D036781:Fimbriae Proteins / Q000276:immunology*; D005434:Flow Cytometry; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology; D008358:Mannose / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D001428:Bacterial Vaccines; D006388:Hemagglutinins; D007155:Immunologic Factors; C000603452:mannosesensitive haemagglutinin, Pseudomonas aeruginosa; D036781:Fimbriae Proteins; D008358:Mannose