Phosphorus utilization and characterization of ileal digesta and excreta from broiler chickens fed diets varying in cereal grain, phosphorus level, and phytase addition.
Both intrinsic and exogenous phytase in poultry feeds can alter phytate utilization and the solubility of P excreted. This experiment determined the effects of feeding diets varying in cereal grain, P concentration and phytase addition on phytate and P utilization and P characterization of ileal digesta and excreta. Twelve treatments, consisting of diets based on corn, wheat, barley, or high fat-low lignin oat and 3 P treatments (low P with 0.30% nonphytate P; low P + 1,000 phytase units of phytase; high P with 0.45% nonphytate P), were fed to 300 broilers using a factorial design. Fresh excreta were collected at 20 and 21 d and ileal digesta was collected at 21 d. Ileal digesta and excreta were analyzed for total P, phytate P and Ca, with P composition determined by solution (31)P nuclear magnetic resonance spectroscopy. Excreta samples were also analyzed for water soluble P (WSP). Apparent ileal digestibility coefficients for phytate P and total P ranged from 0.03 to 0.42 and 0.56 to 0.71, respectively. Diets supplemented with phytase had greater phytate P hydrolysis than unsupplemented diets. Apparent total tract digestibility coefficients for phytate P and total P ranged from 0.10 to 0.73 and 0.43 to 0.61, respectively. Across cereal grains, there was almost a 3-fold increase in total tract phytate P hydrolysis with phytase supplementation. The P composition of ileal digesta was predominantly phytate P (70 to 88% of total P), whereas excreta phytate P ranged from 26 to 76% of total P. Excreta WSP ranged from 3.2 to 7.5 g kg(-1) and was least for the barley diets. There was a 25% reduction in excreta WSP from the high P to the low P + phytase diets and a 37% reduction from the high P to the low P diets. As cereal grain had little influence on phytate digestibility, it is unlikely that intrinsic phytase in grain has much influence on phytate utilization by poultry. Both total P and WSP in excreta were reduced by the low P diet and the low P + phytase diet, irrespective of cereal grain, which reduces the risk of P transfer to water bodies when excreta are applied to land as fertilizer.
Leytem
A B
AB
USDA, Agricultural Research Service, Northwest Irrigation and Soils Research Laboratory, 3793 N 3600 E, Kimberly, ID 83341, USA. April.Leytem@ars.usda.gov
Widyaratne
G P
GP
Thacker
P A
PA
D010832:6-Phytase / Q000737:chemistry / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D002523:Edible Grain / Q000737:chemistry / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D008297:Male; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism*
D010758:Phosphorus; D010832:6-Phytase