title: A ratiometric fluorescent probe for cysteine and homocysteine displaying a large emission shift.
abstract: 4-(1H-phenanthro[9,10-d]imidazol-2-yl)benzaldehyde 1 was rationally designed as a novel ratiometric fluorescent probe for cysteine and homocysteine. Upon addition of cysteine or homocysteine, notably, the probe displayed a very large (125 nm) hypsochromic shift in emission due to switching off intramolecular charge transfer. This large emission wavelength shift may allow probe 1 to be employed for quantitatively detecting Cys/Hcy.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China. weiyinglin@hnu.cn
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.forename: Lingliang
authors.initials: L
authors.lastname: Long
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Zengmei
authors.initials: Z
authors.lastname: Cao
authors.forename: Bingbing
authors.initials: B
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Tan
mesh_terms: D003545:Cysteine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006710:Homocysteine / Q000032:analysis*; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C543093:4-(1H-phenanthro(9,10-d)imidazol-2-yl)benzaldehyde; D005456:Fluorescent Dyes; D007093:Imidazoles; D010618:Phenanthrolines; D006710:Homocysteine; D003545:Cysteine