Fractalkine and TGF-beta1 levels reflect the severity of chronic pancreatitis in humans.
OBJECTIVE
To clarify whether serum chemokine and cytokine levels can become useful biological and functional markers to assess the severity of chronic pancreatitis (CP). This study aimed at clarifying whether serum chemokine and cytokine levels can become useful biological and functional markers to assess the severity of CP.


METHODS
Serum monocyte chemoattractant protein-1 (MCP-1), transforming growth factor beta-1 (TGF-beta1), and soluble type fractalkine (s-fractalkine) concentrations were examined in patients with CP (n = 109) and healthy controls (n = 116). Severity of disease was classified in patients with CP by a staging system. Relationships between stage-specific various clinical factors and serum MCP-1, TGF-beta1, and s-fractalkine levels were investigated. Furthermore, 57 patients with non-alcoholic CP were similarly evaluated in order to exclude influence of alcohol intake.


RESULTS
Patients with CP showed significant higher levels of serum TGF-beta1 and s-fractalkine, but not MCP-1, compared to the controls. Serum TGF-beta1 in the severe stage and s-fractalkine in the mild and the severe stage of CP significantly increased compared to those of controls. However, it was observed that both TGF-beta1 and s-fractalkine levels were affected by alcohol intake. In patients with non-alcoholic CP, serum TGF-beta1 showed significant increase in the moderate stage of CP, and serum s-fractalkine revealed significant increase in the early stage of CP.


CONCLUSIONS
It is suggested that the measurement of serum F-fractalkine is useful to diagnose early-stage CP. Moreover, the combined determination of both, s-fractalkine and TGF-beta1, in human sera may be helpful in evaluating the severity status of CP.
Yasuda
Mikihiko
M
Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Ito
Tetsuhide
T
Oono
Takamasa
T
Kawabe
Ken
K
Kaku
Toyoma
T
Igarashi
Hisato
H
Nakamura
Taichi
T
Takayanagi
Ryoichi
R
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood; D054428:Chemokine CX3CL1 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019512:Pancreatitis, Alcoholic / Q000175:diagnosis / Q000276:immunology*; D050500:Pancreatitis, Chronic / Q000175:diagnosis / Q000276:immunology*; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D053773:Transforming Growth Factor beta1 / Q000097:blood*; D015854:Up-Regulation
D015415:Biomarkers; C111835:CCL2 protein, human; C515538:CX3CL1 protein, human; D018932:Chemokine CCL2; D054428:Chemokine CX3CL1; D053773:Transforming Growth Factor beta1