title: Importance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid-producing yeast Candida bombicola.
abstract: Three cytochrome P450 monooxygenases belonging to the CYP52 family were isolated from the genome of the sophorolipid-producing yeast Candida bombicola using degenerate PCR and genomic walking. One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), whereas the other genes belonged to new groups: CYP52M and CYP52N. CYP52E3 and CYP52N1 turned out to be of no relevance for sophorolipid production, but show clear upregulation when the yeast cells are grown on alkanes as the sole carbon source. On the other hand, CYP52M1 is clearly upregulated during sophorolipid synthesis and very likely takes part in sophorolipid formation.
authors.affiliation: Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium. inge.vanbogaert@ugent.be
authors.forename: Inge N A
authors.initials: IN
authors.lastname: Van Bogaert
authors.forename: Marjan
authors.initials: M
authors.lastname: De Mey
authors.forename: Marjan
authors.initials: M
authors.lastname: Demey
authors.forename: Dirk
authors.initials: D
authors.lastname: Develter
authors.forename: Wim
authors.initials: W
authors.lastname: Soetaert
authors.forename: Erick J
authors.initials: EJ
authors.lastname: Vandamme
mesh_terms: D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004271:DNA, Fungal; D003577:Cytochrome P-450 Enzyme System