Enhanced initial adhesion of osteoblast-like cells on an anatase-structured titania surface formed by H2O2/HCl solution and heat treatment.
OBJECTIVE
This study aims to investigate the effects of anatase-structured titania surface on the response of osteoblast-like cells.


METHODS
Three kinds of titanium (Ti) disks were prepared: the polished Ti (PT), the roughened Ti (RT) formed by sandblasting and acid etching, and the anatase-coated roughened Ti (ART) formed on the RT via H(2)O(2)/HCl solution treatment followed by heating at 400 degrees C. The surface topography of Ti disks was observed under a scanning electron microscope. MC3T3-E1 cells were cultured on the Ti disks. Cell morphology was observed by fluorescence microscopy. Cell adhesion and proliferation were assessed by MTT testing. To evaluate the cell differentiation on RT and ART, the expressions of core-binding factor alpha, osterix, bone sialoprotein, collagen type 1, osteocalcin and alkaline phosphatase-2 were measured by real-time reverse transcriptase polymerase chain reaction.


RESULTS
The three kinds of Ti disks had distinct surface topographic characteristics. The cells on the ART more rapidly displayed well-defined stress fibers and numerous processes and pseudopodi than the cells on the PT and RT. At 1 and 3h after culture, there were more cells adhering to the ART than to PT and RT. Cell proliferation did not differ between groups after 1-5 days of culture. In addition, ART generally enhanced the osteogenic gene expression in a time-dependent manner compared with RT.


CONCLUSIONS
Anatase-structured titania promotes initial adhesion and spreading of osteoblast-like cells, but does not influence cell proliferation. Anatase could also enhance cell differentiation by enhancing osteogenic gene expression.
Yang
Xiao-feng
XF
Hospital of Stomatology, School of Medicine, Zhejiang University, Hangzhou 310006, PR China.
Chen
Yao
Y
Yang
Fan
F
He
Fu-Ming
FM
Zhao
Shi-fang
SF
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D048430:Cell Shape; D024042:Collagen Type I / Q000096:biosynthesis; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D003722:Dental Alloys / Q000494:pharmacology*; D015870:Gene Expression; D006851:Hydrochloric Acid; D006861:Hydrogen Peroxide; D058574:Integrin-Binding Sialoprotein; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000096:biosynthesis; D000076182:Sp7 Transcription Factor; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D014157:Transcription Factors / Q000096:biosynthesis
D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D003722:Dental Alloys; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D012795:Sialoglycoproteins; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; D015675:Osteocalcin; C009495:titanium dioxide; D006861:Hydrogen Peroxide; D014025:Titanium; D006851:Hydrochloric Acid