title: Localization of prolyl oligopeptidase in the thalamic and cortical projection neurons: a retrograde neurotracing study in the rat brain.
abstract: Prolyl oligopeptidase (POP) is a serine endopeptidase which hydrolyses proline-containing peptides shorter than 30-mer. POP is believed to be associated with cognitive functions via neuropeptide cleavage. POP has been also connected to the inositol 1,4,5-triphosphate (IP(3)) signalling but the effects of POP-inhibition to the IP(3) accumulation in vivo are still unclear. However, little is known about the physiological role of POP in the brain. We have previously found that in the rat brain POP was specifically expressed in the pyramidal neurons of the cerebral cortex, particularly in the primary motor and somatosensory cortices, and corresponding projection areas in thalamus. Using a retrograde neurotracer we have now visualized the localization of POP in thalamocortical and corticothalamic projection neurons in ventrobasal complex and medial geniculate nucleus of thalamus and somatosensory/motor and auditory cortices. We observed that both in thalamus and cortex over 50% of projection neurons contained POP. These results support the hypothesis that POP is involved in thalamocortical and corticothalamic signal processing. We also propose, based on our neuroanatomical findings and literature, that POP may take part in the thalamocortical oscillations by interacting with IP(3) signalling in cells.
authors.affiliation: Department of Pharmacology and Toxicology, University of Kuopio, P.O. Box 1627, FI-70211 Kuopio, Finland. Timo.Myohanen@uku.fi
authors.forename: Timo T
authors.initials: TT
authors.lastname: Myöhänen
authors.forename: Tiina M
authors.initials: TM
authors.lastname: Kääriäinen
authors.forename: Aaro J
authors.initials: AJ
authors.lastname: Jalkanen
authors.forename: Marjo
authors.initials: M
authors.lastname: Piltonen
authors.forename: Pekka T
authors.initials: PT
authors.lastname: Männistö
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D000085822:Prolyl Oligopeptidases; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000378:metabolism*; D013266:Stilbamidines / Q000378:metabolism; D013788:Thalamus / Q000166:cytology* / Q000378:metabolism*
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D013266:Stilbamidines; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases