title: Biflavonoids from Daphne feddei and their inhibitory activities against nitric oxide production.
abstract: Bioassay-directed fractionation led to the isolation of two new biflavonoids and 13 known biflavonoids from a sample of the dried stem barks of Daphne feddei. The structures of compounds 1 and 2 were elucidated as 2''-methoxy-daphnodorin C (1) and 2''-methoxy-2-epi-daphnodorin C (2) on the basis of detailed spectroscopic analysis and X-ray crystallography. All 15 biflavonoids were tested for inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 1, 2, 8, 10, 14 and 15 showed varying degrees of inhibitory activities against the production of NO in tested concentration of 25, 50, 75 and 100 microg/ml.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Jin
authors.forename: Jun-Mian
authors.initials: JM
authors.lastname: Tian
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Yan
mesh_terms: D000818:Animals; D044946:Biflavonoids / Q000737:chemistry* / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D032364:Daphne / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013329:Structure-Activity Relationship
chemical_list: C534254:2''-methoxy-2-epi-daphnodorin C; C534253:2''-methoxy-daphnodorin C; D044946:Biflavonoids; D005419:Flavonoids; D008070:Lipopolysaccharides; D009569:Nitric Oxide