[Immunosuppression induced by measles virus in adult patients is not related to CD4+ CD25+ regulatory T cell induction].
OBJECTIVE
To investigate of the relationship of the immunosuppression induced by Measles virus in adult patients and CD4+ CD25+ regulatory T cell.


METHODS
Thirty-four patients with measles and 27 healthy control subjects were included in this study. The whole blood was collected and CD4+ CD25+ cell and FoxP3+ cell were analyzed by flow cytometry, and CD4+ CD25- and CD4+ CD25+ T lymphocytes were isolated from PBMCs of patients with measles or healthy donors, CD4+ CD25- T cells were cultured in absence or presence of anti-CD3, or BCG, or live attenuated MV. The cell culture supernatant was collected after 72 hours and the concentration of IFN-gamma and IL-10 was determined.


RESULTS
Compared to healthy donors, we observed a reduction of the number of white blood cells and lymphocytes in patients with measles, but there was not significantly different in the frequency of CD4+ CD25+ T cells and CD4+ CD25high T cells within the total CD4+ population in the blood. Treg from both measles patients and healthy controls significantly inhibited IFN-gamma production by CD4+ CD25- T cells in response to anti-CD3 stimulation.


CONCLUSIONS
Induction and expansion of Treg may not represent a mechanism involved in the establishment of immune suppression by MV.
Li
Mei-Zhong
MZ
Shenzhen Donghu Hospital, Shenzhen 518020, China.
Xu
Fu-De
FD
Huang
Xue-Huan
XH
Chen
Xin-Chun
XC
Deng
Qi-Wen
QW
Liu
Shui-Teng
ST
Liu
Yan
Y
Xu
Liu-Mei
LM
Wang
Huo-Sheng
HS
Cui
Jian-Jun
JJ
D000293:Adolescent; D000328:Adult; D015704:CD4 Antigens / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008457:Measles / Q000276:immunology* / Q000821:virology; D008459:Measles virus / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit