title: Gating deficits in isolation-reared rats are correlated with alterations in protein expression in nucleus accumbens.
abstract: The isolation-rearing (IR) paradigm, consisting of the social deprivation for 6-9 weeks after weaning, induces a spectrum of aberrant behaviors in adult rats. Some of these alterations such as sensorimotor gating deficits are reminiscent of the dysfunctions observed in schizophrenia patients. Although gating impairments in IR rats have been linked to impairments in the cortico-mesolimbic system, the specific molecular mechanisms underlying this relation are unclear. To elucidate the neurochemical modifications underlying the gating disturbances exhibited by IR rats, we compared their pre-pulse inhibition (PPI) of the acoustic startle reflex with that of socially reared (SR) controls, and correlated this index to the results of proteomic analyses in prefrontal cortex and nucleus accumbens from both groups. As expected, IR rats exhibited significantly lower startle amplitude and PPI than their SR counterparts. Following behavioral testing, IR and SR rats were killed and protein expression profiles of their brain regions were examined using two-dimensional electrophoresis based proteomics. Image analysis in the Coomassie blue-stained gel revealed that three protein spots were differentially expressed in the nucleus accumbens of IR and SR rats. Mass spectrometry (matrix-assisted laser desorption ionization-time of flight and MS/MS) identified these spots as heat shock protein 60 (HSP60), alpha-synuclein (alpha-syn), and 14-3-3 protein zeta/delta. While accumbal levels of HSP60 was decreased in IR rats, alpha-syn and 14-3-3 proteins were significantly increased in IR in comparison with SR controls. Notably, these two last alterations were significantly correlated with different loudness intensity-specific PPI deficits in IR rats. In view of the role of these proteins in synaptic trafficking and dopaminergic regulation, these findings might provide a neurochemical foundation for the gating alterations and psychotic-like behaviors in IR rats.
authors.affiliation: Istituto Sperimentale Italiano L. Spallanzani, Milano, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Roncada
authors.forename: Marco
authors.initials: M
authors.lastname: Bortolato
authors.forename: Roberto
authors.initials: R
authors.lastname: Frau
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Saba
authors.forename: Giovanna
authors.initials: G
authors.lastname: Flore
authors.forename: Alessio
authors.initials: A
authors.lastname: Soggiu
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pisanu
authors.forename: Angela
authors.initials: A
authors.lastname: Amoresano
authors.forename: Andrea
authors.initials: A
authors.lastname: Carpentieri
authors.forename: Paola
authors.initials: P
authors.lastname: Devoto
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology; D003627:Data Interpretation, Statistical; D015180:Electrophoresis, Gel, Two-Dimensional; D017403:In Situ Hybridization; D013058:Mass Spectrometry; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009714:Nucleus Accumbens / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism; D020543:Proteome / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013216:Reflex, Startle / Q000235:genetics* / Q000502:physiology*; D012565:Schizophrenic Psychology; D012934:Social Isolation / Q000523:psychology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D051844:alpha-Synuclein / Q000096:biosynthesis / Q000235:genetics
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome; D051844:alpha-Synuclein