Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies--a comparative study.
Economizing the research protocols by using low cost technologies is the need of laboratories of developing world. Screening of recombinant E. coli colonies is the crucial step in gene cloning and expression studies. In the present study, the cost effectiveness of colony lysis method and colony PCR method in the screening of recombinant E. coli colonies was compared. The colony lysis method was 20 two times more cost effective and less time consuming and can be used to screen the recombinant E. coli colonies in large scale instead of colony PCR method.
Azhahianambi
P
P
Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Ghosh
S
S
Ashok Kumar
C
C
Suryanarayana
V V S
VV
D001431:Bacteriological Techniques / Q000191:economics; D003362:Cost-Benefit Analysis; D004926:Escherichia coli / Q000235:genetics*; D005821:Genetic Techniques / Q000191:economics*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods; D011995:Recombination, Genetic