title: Effect of actin C-terminal modification on tropomyosin isoforms binding and thin filament regulation.
abstract: Tropomyosins, a family of actin-binding regulatory proteins, are present in muscle and non-muscle cells. Multiple tropomyosin (TM) isoforms differ in actin affinity and regulatory properties, but little is known about the molecular bases of these differences. The C-terminus of actin stabilizes contacts between actin subunits in the filament and interacts with myosin and regulatory proteins. The goal of this work was to reveal how structural changes in actin and differences between TM isoforms affect binding between these proteins and affect thin filament regulation. Actin proteolytically truncated by three C-terminal amino acids exhibited 1.2-1.5 fold reduced affinity for non-muscle and smooth muscle tropomyosin isoforms. The truncation increased the cooperativity of myosin S1-induced tropomyosin binding for short tropomyosins (TM5a and TM1b9a), but it was neutral for long isoforms (smTM and TM2). Actin modification affected regulation of actomyosin ATPase activity in the presence of all tropomyosins by shifting the filament into a more active state. We conclude that the integrity of the actin C-terminus is important for actin-tropomyosin interactions, however the increased affinity of tropomyosin binding in the S1-induced state of the filament appears not to be involved in the tropomyosin isoform-dependent mechanism of the actomyosin ATPase activation.
authors.affiliation: Kazimierz Wielki University in Bydgoszcz, Department of Experimental Biology, Chodkiewicza 30, 85-064 Bydgoszcz, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Skórzewski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sliwińska
authors.forename: Danuta
authors.initials: D
authors.lastname: Borys
authors.forename: Apolinary
authors.initials: A
authors.lastname: Sobieszek
authors.forename: Joanna
authors.initials: J
authors.lastname: Moraczewska
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D009130:Muscle, Smooth / Q000378:metabolism; D010369:Pectoralis Muscles / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D014335:Tropomyosin / Q000378:metabolism*
chemical_list: D000199:Actins; D020033:Protein Isoforms; D014335:Tropomyosin