Endothelial progenitor cells in adolescents: impact of overweight, age, smoking, sport and cytokines in younger age.
OBJECTIVE
Endothelial progenitor cells (EPCs) are bone marrow derived pluripotent vascular progenitor cells capable to contribute to re-endothelialization and neovascularization. The number of circulating EPCs has been established as a biomarker of cardiovascular risk and is known to decrease with age. We determined the number of EPCs in teenagers and evaluated the influence of traditional risk factors focusing on overweight.


METHODS
79 male adolescents were enrolled (age 13-17 years; 42 of normal weight: 64.1 +/- 7.6 kg; 37 above the 90th BMI-percentile: 96.9 +/- 20.5 kg). 41 healthy adults served as controls. EPCs were counted by flow cytometry (CD34+/-CD133/KDR). Besides traditional risk factors, cholesterol, and high sensitive CRP different cytokines were determined.


RESULTS
Overweight adolescents have a higher systolic blood pressure, higher hsCRP, higher HbA(1c) and lower HDL. The number of CD34-negative EPCs, but not CD34-positive EPCs is higher in overweight adolescents. The overall level of EPCs is lower in adolescents compared to adults.


CONCLUSIONS
Overweight in adolescents influences EPCs in early life. CD34-negative EPCs might be more sensitive to the early risk profile and may represent a biological marker of occult vascular damage. Beginning insulin resistance, endothelial damage and elevation of EPCs could indicate the higher risk for future cardiovascular disease in obese teenagers.
Jung
Christian
C
Clinic of Internal Medicine I, Friedrich-Schiller-University, Erlanger Allee 101, Jena, Germany. christian.jung@med.uni-jena.de
Fischer
Nicole
N
Fritzenwanger
Michael
M
Thude
Hansjörg
H
Ferrari
Markus
M
Fabris
Marlen
M
Brehm
Bernhard R
BR
Barz
Dagmar
D
Figulla
Hans R
HR
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D018952:Antigens, CD34 / Q000097:blood; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure; D002097:C-Reactive Protein / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000209:etiology*; D016207:Cytokines / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D050177:Overweight / Q000150:complications / Q000503:physiopathology*; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D013177:Sports
D018952:Antigens, CD34; D015415:Biomarkers; D016207:Cytokines; D002097:C-Reactive Protein