[Effect of Escherichia coli lipopolysaccharide on mineralized matrix formation in vitro differentiation human dental pulp cell].
OBJECTIVE
To investigated the effect of Escherichia coli (Ec) LPS on alkaline phosphatase (ALP) activity and expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) genes in vitro differentiation human dental pulp cell.


METHODS
Odontoblast-like cells were cultured, cells exposed to Ec LPS for 12 h, total RNA was isolated and DSPP, OCN transcripts were examined by real-time RT-PCR. ALP kit were used to assessed the changes of ALP activity.


RESULTS
Real-time RT-PCR analysis indicated that Ec LPS induced about a 3.6-fold decrease for DSPP gene and a 1.6-fold decrease for OCN gene in odontoblast-like cells as compared with controls. At the same time, cells treated with LPS could depress ALP activity from (1156.10 +/- 100.60) pmol x h(-1) x ng(-1) down to (884.80 +/- 26.72) pmol x h(-1) x ng(-1).


CONCLUSIONS
These results indicate that exposure of odontoblast-like cells to LPS can alter cells function by downregulating cell markers of odontoblastic activity.
Jiang
Hong-wei
HW
Department of Cariology and Endodontology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
Ling
Jun-qi
JQ
Zeng
Jin-feng
JF
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D004926:Escherichia coli; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008903:Minerals / Q000378:metabolism; D009804:Odontoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism*
D008070:Lipopolysaccharides; D008903:Minerals; D015675:Osteocalcin; D000469:Alkaline Phosphatase