Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene.
Nematode parasites of the genus Trichinella are intracellular and distinct life cycle stages invade intestinal epithelial and skeletal muscle cells. Within the genus, Trichinella spiralis and Trichinella pseudospiralis exhibit species-specific differences with respect to host-parasite complex formation and host immune modulation. Parasite excretory-secretory (ES) proteins play important roles at the host-parasite interface and are thought to underpin these differences in biology. Serine proteases are among the most abundant group of T. spiralis ES proteins and multiple isoforms of the muscle larvae-specific TspSP-1 serine protease have been identified. Recently, a similar protein (TppSP-1) in T. pseudospiralis muscle larvae was identified. Here we report the cloning and characterisation of the full-length transcript of TppSP-1 and present comparative data between TspSP-1 and TppSP-1.
Cwiklinski
Krystyna
K
Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
Meskill
Diana
D
Robinson
Mark W
MW
Pozio
Eduardo
E
Appleton
Judith A
JA
Connolly
Bernadette
B
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D007814:Larva / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000469:parasitology; D057057:Serine Proteases / Q000235:genetics / Q000378:metabolism*; D014234:Trichinella / Q000201:enzymology* / Q000235:genetics
D057057:Serine Proteases