title: Control of neural stem cell adhesion and density by an electronic polymer surface switch.
abstract: Adhesion is an essential parameter for stem cells. It regulates the overall cell density along the carrying surface, which further dictates the differentiation scheme of stem cells toward a more matured and specified population as well as tissue. Electronic control of the seeding density of neural stem cells (c17.2) is here reported. Thin electrode films of poly(3,4-ethylenedioxythiophene) (PEDOT):Tosylate were manufactured along the floor of cell growth dishes. As the oxidation state of the conjugated polymer electrodes was controlled, the seeding density could be varied by a factor of 2. Along the oxidized PEDOT:Tosylate-electrodes, a relatively lower density of, and less tightly bonded, human serum albumin (HSA) was observed as compared to reduced electrodes. We found that this favors adhesion of the specific stem cells studied. Surface analysis experiments, such as photoelectron spectroscopy, and water contact angle measurements, were carried out to investigate the mechanisms responsible for the electronic control of the seeding density of the c17.2 neural stem cells. Further, our findings may provide an opening for electronic control of stem cell differentiation.
authors.affiliation: Molecular Neurobiology, MBB, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
authors.forename: Carmen
authors.initials: C
authors.lastname: Saltó
authors.forename: Emilien
authors.initials: E
authors.lastname: Saindon
authors.forename: Maria
authors.initials: M
authors.lastname: Bolin
authors.forename: Anna
authors.initials: A
authors.lastname: Kanciurzewska
authors.forename: Mats
authors.initials: M
authors.lastname: Fahlman
authors.forename: Edwin W H
authors.initials: EW
authors.lastname: Jager
authors.forename: Pentti
authors.initials: P
authors.lastname: Tengvall
authors.forename: Ernest
authors.initials: E
authors.lastname: Arenas
authors.forename: Magnus
authors.initials: M
authors.lastname: Berggren
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D004583:Electrons; D006801:Humans; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology*; D011108:Polymers / Q000737:chemistry*; D012709:Serum Albumin; D013057:Spectrum Analysis; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties
chemical_list: D011108:Polymers; D012709:Serum Albumin