MLVA genotyping of human Brucella isolates from Peru.
Recent human Brucella melitensis isolates from Peru were genotyped by multiple locus variable number repeat analysis. All 24 isolates originated from hospitalized patients living in the central part of Peru and consisted of six genomic groups comprising two to four isolates and nine unique genotypes. The isolates were most closely related to the two previously genotyped isolates from Mexico, with a maximum distance of 2 to 4. The Peruvian strains were clearly distinct from the East and West Mediterranean groups of B. melitensis genotypes, suggesting that they may constitute a unique Latin American cluster.
Smits
Henk L
HL
KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. h.smits@kit.nl
Espinosa
Benjamin
B
Castillo
Rosa
R
Hall
Eric
E
Guillen
Alfredo
A
Zevaleta
Milagros
M
Gilman
Robert H
RH
Melendez
Paolo
P
Guerra
Carlos
C
Draeger
Angelika
A
Broglia
Alessandro
A
Nöckler
Karsten
K
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics*; D002006:Brucellosis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006041:Goats / Q000382:microbiology; D006801:Humans; D010568:Peru; D016133:Polymerase Chain Reaction; D012008:Recurrence; D012307:Risk Factors; D017422:Sequence Analysis, DNA / Q000379:methods*
D004269:DNA, Bacterial; D005819:Genetic Markers