Detection of cytochrome P450-2A6, -3A5 and -4B1 with real-time polymerase chain reaction in prostate tissue.
Cytochrome P450 (CYP) is a heme-containing enzyme superfamily metabolizing a wide variety of xenobiotics, including drugs and carcinogens. The majority of CYP genes are expressed in the liver, however, some CYP isoforms are also reported for a number of extra hepatic tissues. We analyzed Cytochrome P450-2A6, -3A5 and -4B1 mRNAs using real-time reverse-transcriptase polymerase chain reaction (RT-PCR) in a total of 21 homogenized prostate tissues with or without malignancy. We detected a consistent expression of CYP2A6 and CYP3A5 in all, and of CYP4B1 in some (11/21) of the samples at mRNA level. Neither the histopathological status nor the smoking habit of the individuals affected CYP4B1 expression. Our results reflect possible roles for these particular CYPs in therapy and protection of prostate tissue.
Zencir
Sevil
S
Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Alptekin
Davut
D
Celiktas
Medih
M
Canturk
Pakize
P
Colak
Deniz
D
Caner
Vildan
V
Luleyap
Umit H
UH
Topcu
Zeki
Z
D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D001706:Biopsy; D065637:Cytochrome P-450 CYP2A6; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D008297:Male; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods; D011467:Prostate / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D001189:Aryl Hydrocarbon Hydroxylases; C585710:CYP2A6 protein, human; C510164:CYP3A5 protein, human; D065637:Cytochrome P-450 CYP2A6; D051544:Cytochrome P-450 CYP3A; C095029:cytochrome P-450 CYP4B1; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases