[BFGF entraped in cationic liposomes as adjuvant: optimization of technical parameters].
OBJECTIVE
To test the immune efficiency of bFGF entraped in cationic liposomes as adjuvant in vivo.


METHODS
The technical parameters on encapsulation were tested in each step to gain high encapsulation efficiencies, which included lipid composition, weight ratio of protein and lipids, liposome extrusion, and different conditions of freeze-thawing. The bFGF in cationic liposome, Freund's adjuvant, or PBS were injected (four times) to the four-week old Balb/c mice to test the immune responses. The serum antibody was measured by ELISA 13 days after each injection.


RESULTS
Maximal encapsulation efficiency (about 50%) was achieved through optimized technical parameters. Cationic liposome demonstrated satisfied immune efficiency as adjuvant.


CONCLUSIONS
Cationic liposome is a safe and effective immunological adjuvant.
Zhong
Zhen-hua
ZH
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Mao
Shuang-lin
SL
Yang
Li
L
Yang
Guang-li
GL
Hou
Wen-li
WL
Chen
Xiang
X
Chen
Li-juan
LJ
D000818:Animals; D002412:Cations / Q000737:chemistry / Q000276:immunology; D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000276:immunology*; D005620:Freund's Adjuvant / Q000276:immunology; D007114:Immunization; D008081:Liposomes / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D002412:Cations; D004337:Drug Carriers; D008081:Liposomes; D016222:Fibroblast Growth Factor 2; D005620:Freund's Adjuvant