[Rescue of chimeric rabies virus expressing green fluorescent protein].
Green fluorescent protein (GFP) gene was inserted into the pseudogene (psi) region of genome of rabies virus rHep-Flury strain, and a recombinant rabies virus carrying GFP, designated as HEP-GFP, was rescued by reverse genetics system. It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line. The research indicated that the pseudogene (psi) region in the genome of rHEP-Flury strain, as an independent functional unit in the process of virus assembly, could independently carry and express exogenous genes.
Liu
Xiao-Hui
XH
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642.
Ai
Jun
J
Guo
Xiao-Feng
XF
Sun
Jing-Chen
JC
Liang
Hong-Ru
HR
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D049452:Green Fluorescent Proteins / Q000235:genetics*; D011820:Rabies virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins