Candida albicans cells lacking CaMCA1-encoded metacaspase show resistance to oxidative stress-induced death and change in energy metabolism.
Candida albicans, an opportunistic pathogen, can undergo programmed cell death upon various stimuli, including oxidative stress. In this study, we showed that deletion of CaMCA1, a homologue of Saccharomyces cerevisiae metacaspase YCA1, could both attenuated oxidative stress-induced cell death and caspase activation. Compared to wild-type strain, Camca1Delta mutant showed higher accumulation of trehalose and transcription of the genes related to trehalose biosynthesis (TPS2 and TPS3) under the condition of oxidative stress. Furthermore, lower intracellular ATP concentration and mitochondrial membrane potential, less endogenous reactive oxygen species (ROS) generation were observed in Camca1Delta mutant. Our results suggest that CaMCA1 might mediate the sensitiveness to oxidative stress by affecting energy metabolism in C. albicans.
Cao
Yingying
Y
School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Huang
Shan
S
Dai
Baodi
B
Zhu
Zhenyu
Z
Lu
Hui
H
Dong
Lingling
L
Cao
Yongbing
Y
Wang
Yan
Y
Gao
Pinghui
P
Chai
Yifeng
Y
Jiang
Yuanying
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D050296:Microbial Viability; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D017384:Sequence Deletion; D014199:Trehalose / Q000378:metabolism
D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D014199:Trehalose; D020169:Caspases