title: Effects of hyperglycemia on the modulation of vascular function by perivascular adipose tissue.
abstract: OBJECTIVE
  To study the acute and chronic effect of hyperglycemia on perivascular adipose tissue (PVAT) function in rat aorta.
  
  
  METHODS
  Alterations in PVAT function in rat aorta incubated with 22 mmol/l D-glucose for 30 min and in aorta from streptozotocin (STZ)-induced diabetic rats were studied.
  
  
  RESULTS
  Incubation with D-glucose caused an attenuation of contraction in response to phenylephrine, both in the presence and absence of endothelium, whereas removal of PVAT eliminated this attenuation effect. The presence of PVAT did not affect concentration-related relaxation response of the aorta to carbamylcholine in STZ rats. There was also no difference in the relaxation response of the aorta to carbamylcholine between STZ and control rats. The presence of PVAT, however, caused a higher attenuation of the concentration-dependent contraction to phenylephrine in aorta from STZ rats with intact endothelium as compared with that from control rats. Incubation of the aorta from control rats with Nomega-nitro-L-arginine or carboxy-2-phenyl-4,4,5,5-tetra-methyl-imidazoline-1-oxyl-3-oxide potentiated the contraction of the vessels to phenylephrine, and this potentiation effect was higher in the vessels from STZ rats than control rats when N-nitro-L-arginine was used. Removal of PVAT reduced this potentiation effect and eliminated the difference between the vessels from control and STZ rats.
  
  
  CONCLUSIONS
  Under both acute and chronic conditions, hyperglycemia enhanced the relaxation response of the vessels mediated by PVAT. These new findings provide important information on the mechanism underlying the postprandial effect of hyperglycemia on blood pressure control and the presence of hypotension under chronic hyperglycemia in a type-1 model of diabetes.
authors.affiliation: Smooth Muscle Research Program, McMaster University, Hamilton, Ontario, Canada. rmkwlee@mcmaster.ca
authors.forename: Robert M K W
authors.initials: RM
authors.lastname: Lee
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Su
authors.forename: Geoff
authors.initials: G
authors.lastname: Werstuck
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Gao
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D001013:Aorta, Thoracic / Q000503:physiopathology; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000503:physiopathology*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013311:Streptozocin / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D010656:Phenylephrine; D013311:Streptozocin; D005947:Glucose