title: A large genome center's improvements to the Illumina sequencing system.
abstract: The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Quail
authors.forename: Iwanka
authors.initials: I
authors.lastname: Kozarewa
authors.forename: Frances
authors.initials: F
authors.lastname: Smith
authors.forename: Aylwyn
authors.initials: A
authors.lastname: Scally
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Stephens
authors.forename: Richard
authors.initials: R
authors.lastname: Durbin
authors.forename: Harold
authors.initials: H
authors.lastname: Swerdlow
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Turner
mesh_terms: D000047:Academies and Institutes; D002874:Chromosome Mapping / Q000295:instrumentation*; D004867:Equipment Design; D023281:Genomics / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*