Heterologous expression and purification of the serotonin type 4 receptor from transgenic mouse retina.
Recent breakthroughs in the solution of X-ray structures for G protein-coupled receptors (GPCRs) with diffusible ligands have employed extensively mutated or recombined receptor fusion proteins heterologously expressed in conventional in vitro cell-based systems. While these advances now show that crystallization of non-rhodopsin members of this superfamily can be accomplished, the use of radically modified proteins may limit the relevance of the derived structures for precision-guided drug design. To better enable the study of native GPCR structures, we report here efforts to engineer an in vivo expression system that harnesses the photoreceptor system of the retina to express heterologous GPCRs with native human sequences in a biochemically homogeneous and pharmacologically functional conformation. As an example, we show that the human 5HT4 receptor, when placed under the influence of the mouse opsin promoter and an opsin rod outer segment (ROS) targeting sequence, localized to ROS of transgenic mouse retina. The resulting receptor protein was uniformly glycosylated and pharmacologically intact as demonstrated by immunoblotting and radioligand binding assays. Upon solubilization, the retinal 5HT4 receptor retained the binding properties of its initial state in retinal membranes. With the engineered T7 monoclonal epitope sequence, the solubilized receptor was easily purified by one-step immunoaffinity chromatography and the purified receptor in detergent solution preserved its ligand binding properties. This expression method may prove generally useful for generating functional, high-quality GPCR protein.
Salom
David
D
Polgenix Inc., 11000 Cedar Avenue, Suite 260, Cleveland, Ohio 44106, USA.
Wu
Nan
N
Sun
Wenyu
W
Dong
Zhiqian
Z
Palczewski
Krzysztof
K
Jordan
Steven
S
Salon
John A
JA
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D018390:Gene Targeting; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D055355:Opsins / Q000235:genetics; D044422:Receptors, Serotonin, 5-HT4 / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D012160:Retina / Q000737:chemistry / Q000502:physiology*; D014162:Transfection; D014982:Xenopus laevis
D055355:Opsins; D044422:Receptors, Serotonin, 5-HT4