[Effect of metastasis suppressor gene KAI1 on the proliferation and invasive ability of cervical carcinoma cells].
OBJECTIVE
To explore the effect of metastasis suppressor gene KAI1 on the proliferation and invasive ability of cervical cancer cell line CaSki.


METHODS
pCMV-KAI1 cDNA plasmid was transferred into cervical carcinoma cell line CaSki by liposome, which had low level of endogenous KAI1 expression. The expressions of KAI1 protein and mRNA were determined by immunohistochemistry and real-time fluorescence quantitative PCR (RT-PCR), the proliferation of KAI1-transfected CaSki cells was investigated by MTT assay and the invasive ability of these cells was evaluated by in vitro invasion assays.


RESULTS
After the transfection of pCMV-KAI1 cDNA, the level of KAI1 mRNA and protein expression in CaSki cell were increased (P < 0.05), while the cell proliferation was suppresssed, and the migrative ability of passing through the membrane filte also decreased evidently (P < 0.05).


CONCLUSIONS
The KAI1 metastasis suppressor gene suppressed the ability of proliferation and invasion of cervical cancer cell CaSki in vitro.
Ouyang
Yun-wei
YW
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, China.
Pan
Xiao-ling
XL
Qu
Yi
Y
Peng
Zhi-lana
ZL
Wei
Da-peng
DP
Wang
Xia
X
D049109:Cell Proliferation; D005260:Female; D016147:Genes, Tumor Suppressor / Q000502:physiology; D006801:Humans; D051861:Kangai-1 Protein / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
C497688:CD82 protein, human; D051861:Kangai-1 Protein; D012333:RNA, Messenger