The influence of hyperlipidemia on platelet activity markers in patients after ischemic stroke.
BACKGROUND
To investigate the relationship between hyperlipidemia and platelet activation markers--platelet and soluble P-selectin (sP-selectin), and platelet-derived microparticles (PDMPs)--in patients after ischemic stroke.


METHODS
41 patients after ischemic stroke (>3 months) confirmed by CT were divided into 2 groups: with hyperlipidemia (HL, n = 21) and normolipidemia (NL, n = 20). Twenty healthy subjects served as controls. CD62P-positive platelets and PDMPs in whole blood were analyzed by the use of a flow cytometer and anti-CD61 and anti-CD62P monoclonal antibodies. Platelets were activated by thrombin (0.08 units). The level of sP-selectin in serum was measured by ELISA.


RESULTS
We observed a significantly higher CD62P expression and percentage of CD62P-positive resting and thrombin-activated platelets in the HL as compared to the NL group. The sP-selectin concentration was also significantly higher in HL than NL subjects (p < 0.05). Moreover, we observed a significantly higher percentage of PDMPs in patients after stroke (NL: p < 0.05; HL: p = 0.005) in comparison with the control group.


CONCLUSIONS
Patients after stroke present symptoms of platelet hyperreactivity. HL in the patients may be a risk factor for vascular events due to the increase in platelet activation.
Pawelczyk
Malgorzata
M
Departments of Neurology and Epileptology, Medical University of Lodz, Zeromskiego Str. 113, Lodz, Poland. gosiagalka@poczta.onet.pl
Baj
Zbigniew
Z
Chmielewski
Henryk
H
Kaczorowska
Beata
B
Klimek
Andrzej
A
D000368:Aged; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000503:physiopathology*; D039661:Integrin beta3 / Q000097:blood; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000502:physiology*; D020521:Stroke / Q000097:blood* / Q000503:physiopathology*
D015415:Biomarkers; D039661:Integrin beta3; D019007:P-Selectin