[An experimental study on percutaneous autologous bone marrow mesenchymal stem cell grafting for bone defect].
OBJECTIVE
To investigate in vive osteogenic potential in size-critical bone defect after percutaneous autologous grafting of culture-expanded rabbit autologous BMSCs, osteo-induced BMSCs and combination of both.


METHODS
BMSCs were cultured and then induced with osteogenic supplement (OS) medium. BMSCs with and without OS induction were collected and percutaneously autologously injected respectively into the 15 mm bone defect of 20 experimental rabbit model. The grafts were BMSCs, osteo-induced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, and 0.9% NaCl solution. Osteogenesis at the defect areas were observed by regular radiography, histology and biomechanics.


RESULTS
The group transplanted with BMSCs + osteo-induced BMSCs achieved complete bone healing with medullary cavity united, which showed the largest quantity of new bone measured by X-ray analysis, and also their maximal load were better than those in other groups.


CONCLUSIONS
The bone-forming ability of rabbit osteo-induced BMSCs combined with BMSCs in bone defect is superior to those of BMSCs and osteo-induced BMSCs.
Chang
Yu-Li
YL
Department of Orthopaedics, Beijing Army General Hospital, Beijing 100700, China.
Sun
Tian-Sheng
TS
Zhang
Xiao-Dong
XD
Ma
Zhou-Yong
ZY
Su
Qiang
Q
Ren
Da-Jiang
DJ
Zhang
Li-Ren
LR
Xu
Shao-Ting
ST
D000818:Animals; D001847:Bone Diseases / Q000601:surgery*; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011817:Rabbits; D014182:Transplantation, Autologous