title: Unusual amplification pattern by SSP HLA-B typing led to discovery of a novel HLA-C allele.
abstract: We report here the unique sequence of a novel human leucocyte antigen (HLA)-C allele resulted in amplification of a product with a primer pair used in sequence-specific primer (SSP) HLA-B typing, which led subsequently to identification of a new allele of Cw*0339 by sequence-based typing method. This new allele is closely related to Cw*031101 with only two nucleotide changes from T to G at position 97, and T to C at position 105 in the exon 2 region of HLA-C locus. The first nucleotide substitution causes an amino acid alteration from tyrosine to aspartic acid at amino acid residue 9 (Y to D), while the second one keeps alanine at residue 11 unchanged. Most interestingly, the nucleotide change from T to G at position 97 makes it identical to the 3' end sequence of one SSP of the commercial HLA-B SSP typing kit we were using. In this specific case, it resulted in amplification of a product of HLA-C gene instead of B gene.
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: K R
authors.initials: KR
authors.lastname: Miao
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Pan
authors.forename: H X
authors.initials: HX
authors.lastname: Wu
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhou
authors.forename: M
authors.initials: M
authors.lastname: Pan
authors.forename: M
authors.initials: M
authors.lastname: Xue
authors.forename: S
authors.initials: S
authors.lastname: Fan
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: R C
authors.initials: RC
authors.lastname: Tang
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D015235:HLA-B Antigens; D015236:HLA-C Antigens