title: Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro.
abstract: The present study aimed to evaluate whether the association between a calcium hydroxide paste (Calen paste) and 0.4% chlorhexidine (CHX) affects the development of the osteogenic phenotype in vitro. With rat calvarial osteogenic cell cultures, the following parameters were assayed: cell morphology and viability, alkaline phosphatase activity, total protein content, bone sialoprotein immunolocalization, and mineralized nodule formation. Comparisons were carried out by using the nonparametric Kruskal-Wallis test (level of significance, 5%). The results showed that the association between Calen paste and 0.4% CHX did not affect the development of the osteogenic phenotype. No significant changes were observed in terms of cell shape, cell viability, alkaline phosphatase activity, and the total amount of bone-like nodule formation among control, Calen, or Calen + CHX groups. The strategy to combine Ca(OH)(2) and CHX to promote a desirable synergistic antibacterial effect during endodontic treatment in vivo might not significantly affect osteoblastic cell biology.
authors.affiliation: Department of Pediatric Clinic, Preventive and Social Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. raquel@forp.usp.br
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: da Silva
authors.forename: Mário Roberto
authors.initials: MR
authors.lastname: Leonardo
authors.forename: Léa Assed Bezerra
authors.initials: LA
authors.lastname: da Silva
authors.forename: Larissa Moreira Spinola
authors.initials: LM
authors.lastname: de Castro
authors.forename: Adalberto Luiz
authors.initials: AL
authors.lastname: Rosa
authors.forename: Paulo Tambasco
authors.initials: PT
authors.lastname: de Oliveira
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001857:Bone Matrix / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002126:Calcium Hydroxide / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002710:Chlorhexidine / Q000494:pharmacology*; D004396:Coloring Agents; D004338:Drug Combinations; D005456:Fluorescent Dyes; D007211:Indoles; D058574:Integrin-Binding Sialoprotein; D009930:Organic Chemicals; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000032:analysis; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: C417664:Alexa594; D004396:Coloring Agents; D004338:Drug Combinations; D005456:Fluorescent Dyes; C546873:Ibsp protein, rat; D007211:Indoles; D058574:Integrin-Binding Sialoprotein; D009930:Organic Chemicals; D011506:Proteins; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D012795:Sialoglycoproteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C420643:calen; D053495:Osteopontin; C007293:DAPI; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D002126:Calcium Hydroxide; D002710:Chlorhexidine