X-ray-induced apoptosis of BEL-7402 cell line enhanced by extremely low frequency electromagnetic field in vitro.
This study was designed to test whether extremely low frequency electromagnetic field (ELF-EMF) could enhance the apoptosis-induction effect of X-ray radiotherapy on liver cancer cell line BEL-7402 in vitro. EMF exposure was performed inside an energized solenoid coil. X-ray irradiation was performed using a linear accelerator. Apoptosis rates of BEL-7402 cells were analyzed using Annexin V-Fit Apoptosis Detection kit. Apoptosis rates of EMF group and sham EMF group were compared when combined with X-ray irradiation. Our results suggested that the apoptosis rate of BEL-7402 cells exposed to low doses of X-ray irradiation could be significantly increased by EMF. More EMF exposures obtain significantly higher apoptosis rates than fewer EMF exposures when combined with 2 Gy X-ray irradiation. These findings suggested that ELF-EMF could augment the cell apoptosis effects of low doses of X-ray irradiation on BEL-7402 cells in a synergistic and cumulative way.
Jian
Wen
W
Chemistry and Chemical Engineering School, Central South University, Changsha, China.
Wei
Zhao
Z
Zhiqiang
Cheng
C
Zheng
Fang
F
D017209:Apoptosis / Q000528:radiation effects*; D002460:Cell Line; D004574:Electromagnetic Fields; D014965:X-Rays