title: CD4+ T cells have a key instructive role in educating dendritic cells in allergy.
abstract: BACKGROUND
  Dendritic cells (DCs) are central in allergy as regulators of the Th1/Th2 balance. We have recently demonstrated a unique transcriptional profile of DCs in patients with ongoing allergy compared with healthy subjects and shown that crosstalk between DCs and memory T cells affects the transcriptional profile of T cells. However, the transcriptional profile of DCs educated by T cells in allergy is unknown.
  
  
  METHODS
  In the present study, we have examined the transcriptional profiles of DCs after stimulation with grass pollen allergens, Phleum pratense and coculture with autologous CD4+ memory T cells using high-density microarray. Protein analysis was performed using flow cytometry and recombinant antibody protein microarrays. Patients with allergic rhinitis and healthy subjects were compared.
  
  
  RESULTS
  The results reveal a distinct T-cell-induced DC profile in atopic individuals. Accordingly, about 170 genes were upregulated and 40 genes downregulated. For example, the chemokine receptor CXCR4 and the tumor necrosis factor receptor CD30 were upregulated in DCs derived from atopic donors, and this could also be verified at the protein level.
  
  
  CONCLUSIONS
  We conclude that crosstalk between CD4+ memory T cells and autologous DCs induces transcriptional reprogramming in DCs. This finding suggests that T cells have a key instructive role in educating DCs in Th2-type responses.
authors.affiliation: Department of Immunotechnology, Lund University, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Larsson
authors.forename: Malin
authors.initials: M
authors.lastname: Lindstedt
authors.forename: Kristina
authors.initials: K
authors.lastname: Lundberg
authors.forename: Linda
authors.initials: L
authors.lastname: Dexlin
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
authors.forename: Magnus
authors.initials: M
authors.lastname: Korsgren
authors.forename: Lennart
authors.initials: L
authors.lastname: Greiff
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
mesh_terms: D000485:Allergens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology*; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D040081:Protein Array Analysis
chemical_list: D000485:Allergens; D016207:Cytokines