Cell based therapy for osteoarthritis in a sheep model: gross and histological assessment.
This study was to determine if autologous bone marrow mesenchymal stem cells (BMSCs) cultured in chondrogenic medium could repair surgically induced osteoarthritis. Sheep BMSCs were cultured in medium containing 5ng/ml TGFbeta3 + 50ng/ml IGF-1 for three weeks. The cultured cells were then suspended at density of 2x10(6) cell/ml and injected intraarticularly into the osteoarthritic knee joint. After six weeks, the distal head of the femur and the proximal tibial plateau were removed and stained with H&E. The results indicated that knee joints treated with autologous BMSCs cultured in chondrogenic medium showed clear evidence of articular cartilage regeneration in comparison with other groups.
Alfaqeh
H
H
Department of Physiology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
Norhamdan
M Y
MY
Chua
K H
KH
Chen
H C
HC
Aminuddin
B S
BS
Ruszymah
B H I
BH
D000818:Animals; D001853:Bone Marrow; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D005269:Femur / Q000473:pathology; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D010003:Osteoarthritis / Q000473:pathology / Q000628:therapy*; D044968:Regenerative Medicine; D034561:Sheep, Domestic; D013977:Tibia / Q000473:pathology; D014182:Transplantation, Autologous