title: Promotion of mouse two-stage skin carcinogenesis by diacylglycerol-rich edible oil.
abstract: Tumor promotion potential of diacylglycerol (DAG)-rich edible oil was examined using a two-stage mouse skin carcinogenesis model initiated with 7,12-dimethylbenz[a]anthracene (DMBA). Topical treatment with 75 mg DAG oil once a day for 5 days/week for 35 weeks caused papillomas in 4 of 23 (17%) DMBA-treated female ICR mice, while DMBA initiation alone and DAG treatment without DMBA initiation did not induce any skin tumors. Doubling the daily treatment (twice a day x 5 days/week) at doses of 75 and 30 mg caused both papillomas and squamous cell carcinomas after DMBA initiation, the incidences of tumors being 48% (12/25) and 44% (11/25), respectively, significantly higher than the 4% (1/23) in the DMBA+ 85 mg triacylglycerol group and 0% (0/24) in the DMBA+ vehicle-treated group. The results indicate that DAG-rich oil has promoting potential for skin carcinogenesis, and thus, further investigations of its tumor-promoting potential in other organs are warranted.
authors.affiliation: Cancer Prevention Basic Research Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Takasuka
authors.forename: Mami
authors.initials: M
authors.lastname: Takahashi
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Hori
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Kitahashi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Iigo
authors.forename: Toshio
authors.initials: T
authors.lastname: Imai
authors.forename: Naoki
authors.initials: N
authors.lastname: Yoshimi
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugimura
authors.forename: Keiji
authors.initials: K
authors.lastname: Wakabayashi
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced; D002471:Cell Transformation, Neoplastic; D004075:Diglycerides / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005446:Fluocinolone Acetonide / Q000494:pharmacology; D019787:Linoleic Acid / Q000494:pharmacology; D051379:Mice; D009821:Oils; D019301:Oleic Acid / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D004075:Diglycerides; D005227:Fatty Acids; D009821:Oils; D005446:Fluocinolone Acetonide; D019301:Oleic Acid; D015127:9,10-Dimethyl-1,2-benzanthracene; D019787:Linoleic Acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate