Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts.
Induced pluripotent stem (iPS) cells can be generated from somatic cells by transduction with several transcription factors in mouse and human. However, direct reprogramming in other species has not been reported. Here, we generated monkey iPS cells by retrovirus-mediated introduction of monkey transcription factors OCT4, SOX2, KLF4, and c-MYC.
Liu
Haisong
H
Zhu
Fangfang
F
Yong
Jun
J
Zhang
Pengbo
P
Hou
Pingping
P
Li
Honggang
H
Jiang
Wei
W
Cai
Jun
J
Liu
Meng
M
Cui
Kai
K
Qu
Xiuxia
X
Xiang
Tingting
T
Lu
Danyu
D
Chi
Xiaochun
X
Gao
Ge
G
Ji
Weizhi
W
Ding
Mingxiao
M
Deng
Hongkui
H
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D047108:Embryonic Development / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008253:Macaca mulatta / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014161:Transduction, Genetic / Q000379:methods*
D015415:Biomarkers; D003470:Culture Media; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors