A satellite-like sequence, representing a "clone gap" in the human genome, was likely involved in the seeding of a novel centromere in macaque.
Although the human genome sequence is generally considered "finished", the latest assembly (NCBI Build 36.1) still presents a number of gaps. Some of them are defined as "clone gaps" because they separate neighboring contigs. Evolutionary new centromeres are centromeres that repositioned along the chromosome, without marker order variation, during evolution. We have found that one human "clone gap" at 18q21.2 corresponds to an evolutionary new centromere in Old World Monkeys (OWM). The partially sequenced gap revealed a satellite-like structure. DNA stretches of the same satellite were found in the macaque (flanking the chromosome 18 centromere) and in the marmoset (New World Monkey), which was used as an outgroup. These findings strongly suggested that the repeat was present at the time of novel centromere seeding in OWM ancestor. We have provided, therefore, the first instance of a specific sequence hypothesized to have played a role in triggering the emergence of an evolutionary new centromere.
Carbone
Lucia
L
Children's Hospital Research Institute, Oakland, CA, 94609, USA.
D'addabbo
Pietro
P
Cardone
Maria Francesca
MF
Teti
Maria Grazia
MG
Misceo
Doriana
D
Vessere
Gery M
GM
de Jong
Pieter J
PJ
Rocchi
Mariano
M
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002520:Cercopithecidae / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D019143:Evolution, Molecular; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008251:Macaca / Q000235:genetics*; D008957:Models, Genetic; D013045:Species Specificity
D017931:DNA Primers; D004276:DNA, Satellite