Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs.
The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment was started. Salivary chromogranin A concentrations were measured using an enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary chromogranin A secretion, and no differences in salivary chromogranin A concentrations measured every 4 h were demonstrated during the 24-h cycle in dogs.
Kanai
Kazutaka
K
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato, University, Towada, Aomori 034-8628, Japan.
Hino
Mariko
M
Hori
Yasutomo
Y
Nakao
Ruriko
R
Hoshi
Fumio
F
Itoh
Naoyuki
N
Higuchi
Seiichi
S
D000818:Animals; D053379:Chromogranin A / Q000032:analysis* / Q000378:metabolism*; D002940:Circadian Rhythm; D004285:Dogs / Q000502:physiology*; D012463:Saliva / Q000737:chemistry*
D053379:Chromogranin A