title: Hollow spherical nanostructured polydiphenylamine for direct electrochemistry and glucose biosensor.
abstract: Nanostructured, hollow spheres of polydiphenylamine (HS-PDPA) are prepared through a "soft template assisted self-assembly" approach. An enzymatic glucose biosensor is fabricated through immobilizing glucose oxidase (GOx) into HS-PDPA matrix. The HS-PDPA-GOx electrode exhibits a pair of well-defined reversible redox peaks with a fast heterogeneous electron transfer rate. At an applied potential of +0.65V, HS-PDPA-GOx electrode possesses high sensitivity (1.77 microAmM(-1)cm(-2)), stability and reproducibility towards glucose. The amperometric current response of HS-PDPA-GOx to glucose is linear in the concentration range between 1 and 28 mM with a detection limit of 0.05 mM (S/N=3). Also, HS-PDPA-GOx electrode shows high selectivity towards glucose in the presence of ascorbic acid, uric acid and acetaminophen at their maximum physiological concentrations.
authors.affiliation: Department of Chemistry Graduate School, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: P
authors.initials: P
authors.lastname: Santhosh
authors.forename: K M
authors.initials: KM
authors.lastname: Manesh
authors.forename: S
authors.initials: S
authors.lastname: Uthayakumar
authors.forename: A I
authors.initials: AI
authors.lastname: Gopalan
authors.forename: K-P
authors.initials: KP
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004151:Dipeptides; D004563:Electrochemistry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D008839:Microelectrodes; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004151:Dipeptides; D004800:Enzymes, Immobilized; D011108:Polymers; C026650:phenylalanylphenylalanine; D010649:Phenylalanine; D005949:Glucose Oxidase; D005947:Glucose