A rapid 3% polyacrylamide slab gel electrophoresis method for high through put screening of LDL phenotype.
BACKGROUND
Small dense LDL is reported to be associated with increased coronary artery disease risk by various epidemiological studies. The gold standard for separation and identification of LDL subtypes in plasma is ultracentrifugation which is a lengthy procedure and difficult to perform. Various other methods like NMR, HPLC, gradient gel electrophoresis (GGE) have been reported for LDL sub fractionation all of which require specialized equipments and expertise. We report here a high throughput 3% polyacrylamide slab gel electrophoresis method (PASGE) for sub fractionation of LDL which was compared with GGE, a commonly used method for LDL sub fractionation.


RESULTS
The 3% PASGE method compared well with the GGE method There was a good correlation between LDL particle diameter identified by the PASGE and GGE (Pearson correlation coefficient = 0.950). A 100% concordance was found when samples were classified as per LDL phenotypes in subjects with A and B phenotype by the two methods with the concordance being 66% in subjects with intermediate (I) phenotype. The electrophoresis apparatus was optimized and designed for running twenty eight samples at a time compared to twelve to fourteen by the conventional PASGE and eight to twelve by disc electrophoresis.


CONCLUSIONS
The rapid 3% polyacrylamide slab gel electrphoresis method developed is simple to perform, cost-effective and can be used for the identification LDL sub fractionation and phenotyping in large epidemiological studies.
Singh
Yogendra
Y
Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Ansari nagar, New Delhi-110029, India. ydv.yogesh@gmail.com
Lakshmy
Ramakrishnan
R
Gupta
Ruby
R
Kranthi
Vemparala
V
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008077:Lipoproteins, LDL / Q000032:analysis*; D010641:Phenotype
D008077:Lipoproteins, LDL