title: Surface-enhanced Raman scattering tags for rapid and homogeneous detection of circulating tumor cells in the presence of human whole blood.
abstract: A novel, homogeneous SERS-based cell detection assay was developed for rapid and direct enumeration of circulating tumor cells in the presence of whole blood. Magnetic beads and SERS tags were respectively conjugated to EpCAM and her2 antibodies for the capture and detection of approximately 50 tumor cells/mL in the presence of whole blood in less than 1 h.
authors.affiliation: Oxonica Inc., 325 East Middlefield Road, Mountain View, California 94043, USA. mike_sha168@yahoo.com
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Sha
authors.forename: Hongxia
authors.initials: H
authors.lastname: Xu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Natan
authors.forename: Remy
authors.initials: R
authors.lastname: Cromer
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry; D017209:Apoptosis; D001769:Blood / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D012542:Scattering, Radiation; D015398:Signal Transduction; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule