Scalable production of pancreatic islet progenitors for diabetes cell therapies.
Embryonic stem cells (ESCs) can be an inexhaustible source of islet cells for transplantation. Previously published protocols have been characterized by low differentiation efficiency. In this study, we developed a scalable system for the growth and differentiation of hESCs towards pancreatic islets. Our results showed that hESCs can be grown on microcarriers to a larger scale and directed to differentiate into pancreatic progenitor endoderm cells. This culture system would represent an economical differentiation protocol that can be scaled-up to meet the demand in islet transplantation.
Lock
L T
LT
Department of Chemical and Biological Engineering, State University of New York, USA.
Tzanakakis
E S
ES
D003920:Diabetes Mellitus / Q000628:therapy*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D010865:Pilot Projects; D013234:Stem Cells / Q000166:cytology
D007328:Insulin