Administration of high doses of caffeine increases alveolar bone loss in ligature-induced periodontitis in rats.
BACKGROUND
Although some studies have reported a negative influence of caffeine on bone metabolism, there is no information about its effect on the progression of periodontitis. The aim of this study was to evaluate the effect of high doses of caffeine on ligature-induced periodontitis in rats.


METHODS
Twenty-two Wistar rats were assigned to one of the following groups: non-caffeine group (n = 12) = animals without caffeine ingestion; caffeine group (n = 10) = animals ingesting 10 mg/100 g body weight/day of caffeine via drinking water for 56 days. Two weeks after the beginning of caffeine intake, one of the mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Forty-two days later, the animals were sacrificed, and the specimens were processed to obtain decalcified sections. The area of periodontal ligament and/or bone loss in the furcation region of the first molars was histometrically determined.


RESULTS
Caffeine intake did not have a direct effect on the alveolar bone loss in unligated teeth. But on the ligated tooth, a greater area of bone loss was observed in the animals that ingested caffeine compared to those that did not (P <0.05).


CONCLUSIONS
The present study demonstrated that daily intake of high doses of caffeine may enhance ligature-induced periodontitis progression.
Bezerra
Joyce Pinho
JP
Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
da Silva
Luiz Ricardo Ferreira
LR
de Alvarenga Lemos
Vanessa Adélia
VA
Duarte
Poliana Mendes
PM
Bastos
Marta Ferreira
MF
D016301:Alveolar Bone Loss / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D002110:Caffeine / Q000008:administration & dosage* / Q000494:pharmacology; D000697:Central Nervous System Stimulants / Q000008:administration & dosage* / Q000494:pharmacology; D018450:Disease Progression; D017823:Furcation Defects / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D008026:Ligation / Q000295:instrumentation; D008297:Male; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D010518:Periodontitis / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000697:Central Nervous System Stimulants; D002110:Caffeine