title: Improvement of Yarrowia lipolytica lipase production by fed-batch fermentation.
abstract: Two different types of fed-batch fermentation were investigated to improve production yields of the Lip2 extracellular lipase in Y. lipolytica mutant-strain LgX64.81 grown in a 20l bioreactor. Compare to batch cultures, culture feeding with the complete medium led to a 2-fold increased lipase production (2016 +/- 76 U ml(-1)) whereas addition of a combination of glucose and olive oil led to a 3-fold increase. The high level of lipase production obtained on glucose media with Y. lipolytica LgX64.81 could be related to its phenotype, i.e., a lower sensibility to glucose catabolite repression due to a modification in the level of HXK1 expression.
authors.affiliation: Centre Wallon de Biologie Industrielle, Service de Technologie Microbienne, Université de Liège, Boulevard du Rectorat, Batiment 40, Liège, Belgium. pfickers@ulg.ac.be
authors.forename: Patrick
authors.initials: P
authors.lastname: Fickers
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Destain
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
mesh_terms: D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D008049:Lipase / Q000096:biosynthesis*; D025062:Yarrowia / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; C408971:LIP2 protein, Yarrowia lipolytica; D008049:Lipase; D005947:Glucose