RNA silencing mediated by direct repeats in maize: a potential tool for functional genomics.
It has been shown in tobacco and Arabidopsis that transgenes with multiple direct repeats induce RNA silencing at high frequency. In this study, we tried to establish a direct repeat-induced RNA silencing system in maize and evaluate whether it can be developed as a high throughput tool for functional genomics. Our results showed that the construct phC4, which carries four direct repeats of a chloramphenicol acetyl-transferase (CAT) gene, was able to induce silencing of itself with high efficiency in maize. Using a transient expression system, we further demonstrated that construct phC3G with a beta-glucuronidase (GUS) gene located downstream of three direct repeats of CAT gene silenced not only itself in maize calli but also an "endogenous" GUS gene, which was stably expressed in maize calli. Most importantly, when constructs with the maize iojap (ij) gene inserted in either sense or antisense orientation into the downstream of four direct repeats of CAT gene were transformed into maize plants, co-suppression of endogenous and transgenic ij genes was detected in majority of transgenic maize plants. Our co-suppression results suggest that with improvements, this new approach has the potential to become an efficient research tool for high throughput functional genomics.
Xu
Xiuping
X
State Key Laboratory for Agro-biotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, People's Republic of China.
Zhu
Dengyun
D
Zhao
Qian
Q
Ao
Guangming
G
Ma
Chonglie
C
Yu
Jingjuan
J
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D017343:Genes, Plant; D023281:Genomics / Q000379:methods*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D035261:Plant Components, Aerial / Q000166:cytology; D030821:Plants, Genetically Modified; D018087:Plastids / Q000648:ultrastructure; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D034741:RNA, Small Interfering; D014764:Viral Proteins; D015500:Chloramphenicol O-Acetyltransferase; D005966:Glucuronidase; D003546:Cysteine Endopeptidases