Mannosylated self-assembled structures for molecular confinement and gene delivery applications.
This paper reports self-assembly of a lysine conjugated with a biantennary mannose to form spherical structures. These supramolecular structures are found to be hollow in nature and they afford effective encapsulation of alkaline phosphatase enzyme, plasmid DNA and a GFP reporter gene, which was transfected in COS-7 cells. Loaded hollow structures also get disrupted upon mild sonication, releasing encapsulated molecules thereby illustrating their potential for confinement and delivery applications.
Gour
Nidhi
N
Department of Chemistry, Indian Institute of Technology-Kanpur, Kanpur 208016 (UP), India.
Purohit
Chandra Shekhar
CS
Verma
Sandeep
S
Puri
Rajat
R
Ganesh
Subramaniam
S
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000235:genetics; D008081:Liposomes; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D008362:Mannosides / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D013010:Sonication
D008081:Liposomes; D008362:Mannosides; C534483:bismannosylated lysine; D049452:Green Fluorescent Proteins; D008239:Lysine; D008358:Mannose