Modulation of macrophage functions by compounds isolated from Zingiber officinale.
Bioactivity-guided fractionation of Zingiber Officinale (zingiberaceae) led us to isolate 14 compounds, -gingerol ( 1), -gingerol ( 2), -gingerol ( 3), -gingerol ( 4), -paradol ( 5), -shogaol ( 6), -shogaol ( 7), 1-dehydro- -gingerdione ( 8), -gingerdione ( 9), hexahydrocurcumin ( 10), tetrahydrocurcumin ( 11), gingerenone A ( 12), 1,7-bis-(4' hydroxyl-3' methoxyphenyl)-5-methoxyhepthan-3-one ( 13), and methoxy- -gingerol ( 14). Using the RAW 264.7 cell line, the inhibitory effects on nitric oxide production induced by lipopolysaccharide and the stimulatory effects on phagocytosis of these compounds were evaluated. Compounds 7, 8, and 9 significantly decreased lipopolysaccharide-induced nitric oxide production, and compounds 7 and 8 significantly reduced inducible nitric oxide synthase expression. Among them, compound 8 also showed significant stimulatory effects on phagocytosis.
Koh
Eun Mi
EM
Kim
Hye Jin
HJ
Kim
Sohee
S
Choi
Woo Hyuck
WH
Choi
Yeon Hee
YH
Ryu
Shi Yong
SY
Kim
Young Sup
YS
Koh
Woo Suk
WS
Park
Shin-Young
SY
D000818:Animals; D002396:Catechols / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D036381:Diarylheptanoids / Q000737:chemistry / Q000494:pharmacology; D005233:Fatty Alcohols / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D020939:Zingiber officinale / Q000737:chemistry*; D006139:Guaiacol / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D010587:Phagocytosis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D002396:Catechols; D036381:Diarylheptanoids; D005233:Fatty Alcohols; D008070:Lipopolysaccharides; D010936:Plant Extracts; C000604237:gingerenone A; D009569:Nitric Oxide; D006139:Guaiacol; C040115:shogaol; C007845:gingerol; D052247:Nitric Oxide Synthase Type II; C050872:gingerdione