title: Novel cryoprecipitate for  wound healing and skin  grafts in rats.
abstract: The authors sought to evaluate the ability of locally administered enhanced cryoprecipitate (eCryo) to improve the wound healing of split thickness skin grafts (STSG) and their donor sites. An STSG (5 x 5 cm) was harvested on the back of 30 rats and divided into four areas that were then treated in one of the following groups: A: 'standard' dressing without STSG; B: eCryo without STSG; C: eCryo with STSG coverage and D: STSG alone. Macroscopic and histological assessments (histomorphometric grading scale and cellular composition) were evaluated at days 7, 14, 21 and 28 for wound healing. All wound beds as well as STSGs healed well without any complications. Eighty per cent of the STSG showed a histological graft take of >75% after 28 days. There were no statistically significant differences of macroscopic or histological results between the groups at any time point. Preparation of eCryo is easy and effective. Its use as an adhesive for STSGs is safe and shows similar results as controls. The theoretical benefits of eCryo did not show significant differences. Possible reasons as well as important findings for future research on wound healing are discussed.
authors.affiliation: Aesthetic & Plastic Surgery Institute, University of California, Irvine, Orange, CA 92868-3298, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Scholz
authors.forename: Joshua
authors.initials: J
authors.lastname: Waltzman
authors.forename: Garrett A
authors.initials: GA
authors.lastname: Wirth
authors.forename: Senait W
authors.initials: SW
authors.lastname: Dyson
authors.forename: William J
authors.initials: WJ
authors.lastname: Owens
authors.forename: Edward
authors.initials: E
authors.lastname: Shanbrom
authors.forename: Gregory R D
authors.initials: GR
authors.lastname: Evans
mesh_terms: D000279:Administration, Cutaneous; D000704:Analysis of Variance; D000818:Animals; D001458:Bandages; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005169:Factor VIII / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005340:Fibrinogen / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008297:Male; D009336:Necrosis / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012449:Safety; D017592:Skin Care / Q000379:methods; D016038:Skin Transplantation; D014945:Wound Healing / Q000187:drug effects*
chemical_list: C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005340:Fibrinogen