In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system.
We demonstrate the feasibility of detecting individual tumor-infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high-resolution surface coil. Using a recently developed high-temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 microm)(3) at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin-expressing tumors by transferred OVA antigen-specific cytotoxic lymphocytes with low iron load.
Smirnov
Pierre
P
Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7-Denis Diderot, Paris, France.
Poirier-Quinot
Marie
M
Wilhelm
Claire
C
Lavergne
Elise
E
Ginefri
Jean-Christophe
JC
Combadière
Béhazine
B
Clément
Olivier
O
Darrasse
Luc
L
Gazeau
Florence
F
D000818:Animals; D002460:Cell Line; D003287:Contrast Media; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005290:Ferric Compounds; D007089:Image Enhancement / Q000295:instrumentation*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods*; D008280:Magnetics / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000473:pathology*; D020556:Neutrophil Infiltration; D013601:T-Lymphocytes / Q000473:pathology*
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide