The investigation on the value of repeat and combination test of ACA and anti-beta2-GPI antibody in women with recurrent spontaneous abortion.
OBJECTIVE
In order to investigate the value of anticardiolipin antibodies (ACA) and anti-beta2-GPI antibodies detection in screening autoimmune type recurrent spontaneous abortion and its clinic application in antiphospholipid syndrome diagnosis, we adopt repeat combined ACA and anti-beta2-GPI antibodies detection in this study.


METHODS
Sera were collected from patients and work-up was done for detection of ACA and anti-beta2-GPI antibodies by enzyme-linked immunosorbent assay (ELISA). The work-up was done for detection of antibodies once in every 6 weeks for 14 times consecutively.


RESULTS
The repeated and combined detection of ACA and anti-beta2-GPI antibodies detection could raise the positivity rate up to 21.8% (P < 0.05) in comparison with positive for ACA alone (14.1%), positive for anti-beta2-GPI alone (3.1%), and concurrently positive for both ACA and anti-beta2-GPI antibodies (4.6%). In 91 confirmed positive antiphospholipid antibodies (APA) patients, with more frequent screening for ACA and anti-beta2-GPI antibodies, more patients with APA were found. The positive rate of five and more screenings was over 81.32%, which was statistically significant (P < 0.05), in comparison with that of four or less screenings (68.13%).


CONCLUSIONS
Our data implied that it would be appropriate to take over five or more screenings of combined ACA and anti-beta2-GPI antibodies detection in suspect patients to facilitate the positive diagnostic rate for autoimmune type RSA.
Bao
Shi Hua
SH
Department of Obstetrics and Gynecology, Renji Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
Wang
Xi Peng
XP
Lin
Qi De
QD
Di
Wen
W
Xu
Liang
L
Ding
Chuan Wei
CW
D000026:Abortion, Habitual / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000022:Abortion, Spontaneous / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D011247:Pregnancy; D053482:beta 2-Glycoprotein I / Q000097:blood*
D017153:Antibodies, Anticardiolipin; D053482:beta 2-Glycoprotein I