The structure of the efflux pump AcrB in complex with bile acid.
Gastrointestinal bacteria, like Escherichia coli, must remove bile acid to survive in the gut. Bile acid removal in E. coli is thought to be mediated primarily by the multidrug efflux pump, AcrB. Here, we present the structure of E. coli AcrB in complex with deoxycholate at 3.85 A resolution. All evidence suggests that bile acid is transported out of the cell via the periplasmic vestibule of the AcrAB-TolC complex.
Drew
David
D
Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London, UK.
Klepsch
Mirjam M
MM
Newstead
Simon
S
Flaig
Ralf
R
De Gier
Jan-Willem
JW
Iwata
So
S
Beis
Konstantinos
K
D018360:Crystallography, X-Ray; D003840:Deoxycholic Acid / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry* / Q000378:metabolism; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism
D003840:Deoxycholic Acid; D029968:Escherichia coli Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D050505:Mutant Proteins; C083496:AcrB protein, E coli