Potential core species and satellite species in the bacterial community within the rabbit caecum.
A bacteria library was constructed from the caecum of a rabbit maintained under standard conditions. The complete gene 16S rRNA gene was sequenced. The 228 clones obtained were distributed in 70 operational taxonomic units (OTUs). The large majority of the OTUs were composed of one or two clones and seven OTUs contained half of the sequences. Fourteen sequences had high similarity to the sequence already registered in databases (threshold of 97%). Only one of these sequences has been identified as Variovorax sp. (99% identity). Units were distributed mainly (94%) in the Firmicutes phylum. Three sequences were related to Bacteroidetes. Nine clusters were defined in the phylogenic tree. A great diversity of caecal bacteria of the rabbit was shown. Half of the sequences generated in this library were distributed in the phylogenetic tree near the sequences characterized previously in rabbit caecum (potential core species), and the other half of the sequences were well separated (satellite species).
Monteils
Valérie
V
INRA, Université de Toulouse, UMR 1289, Tissus Animaux, Nutrition, Digestion, Ecosystème et Métabolisme, Chemin de Borde-Rouge, Auzeville,Castanet-Tolosan, France. valerie.monteils@ensat.fr
Cauquil
Laurent
L
Combes
Sylvie
S
Godon
Jean-Jacques
JJ
Gidenne
Thierry
T
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D044822:Biodiversity; D002432:Cecum / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011817:Rabbits / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S