title: Characterization of multidrug-resistant and metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates from a paediatric clinic in China.
abstract: BACKGROUND
  In the present study, we characterized multidrug-resistant Pseudomonas aeruginosa (MDRP) clinical isolates from a paediatric facility and investigated the types and features of the metallo-beta-lactamases (MBLs) produced by carbapenem-resistant strains.
  
  
  METHODS
  Four hundred and ninety-eight strains of Pseudomonas aeruginosa were isolated from patients at Beijing Children's Hospital between January 2005 and December 2006. The minimal inhibition concentrations (MICs) of the strains for 13 antibiotics were measured. A combination of the E test and PCR amplification/DNA sequencing was used to define the carbapenem-resistant strains.
  
  
  RESULTS
  We found that 24.1% (120/498) of the isolates were MDRP. The frequencies of resistance to imipenem and meropenem were 34.2% and 35.8%, respectively, and the MIC50 and MIC90 values for the two antibiotics were identical at 4 microg/ml and 32 microg/ml, respectively. The detection rate for carbapenem resistance was 49.2% (59/120). Among the 59 carbapenem-resistant Pseudomonas aeruginosa strains, 39 (66.1%) were positive for the MBL genotype; 35 (89.7%) strains carried the bla(IMP) gene and 4 (10.3%) strains carried the bla(VIM) gene. Neither bla(SPM) nor bla(GIM) was amplified from any of the 59 isolates. DNA sequencing revealed that IMP-1 was present in 35 IMP-producing isolates and VIM-2 was detected in four VIM-producing isolates.
  
  
  CONCLUSIONS
  These MDRP isolates exhibited high frequencies of resistance to carbapenems among clinical isolates from a paediatric facility in Beijing, China. The production of MBL appears to be an important mechanism for carbapenem resistance in Pseudomonas aeruginosa.
authors.affiliation: Microbiology Laboratory, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
authors.forename: Xi-Wei
authors.initials: XW
authors.lastname: Xu
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Song
authors.forename: Ping
authors.initials: P
authors.lastname: Lü
authors.forename: Sang-Jie
authors.initials: SJ
authors.lastname: Yu
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Yang
authors.forename: Xu-Zhuang
authors.initials: XZ
authors.lastname: Shen
mesh_terms: D015780:Carbapenems / Q000494:pharmacology; D002648:Child; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics
chemical_list: D015780:Carbapenems; D001618:beta-Lactamases