Tracheal instillation of diesel exhaust particles component causes blood and pulmonary neutrophilia and enhances myocardial oxidative stress in mice.
The influences of inhaling particulate air-pollutants on hematopoiesis and myocardial oxidative stress were investigated in mice by intratracheal instillation (IT) of diesel exhaust particles (DEP), its dichloromethane soluble-component (DMSC) or residual particle-component (RPC). After IT, time courses of cytokine levels in bronchial alveolar lavage fluid (BALF), peripheral blood cell count, myocardial myeloperoxidase (MPO) activity and myocardial chemokine levels were observed for 24 hr. RPC caused sustained blood neutrophilia while that caused by DEP and DMSC was transient. RPC also caused sustained elevations of granulocyte colony-stimulating factor (G-CSF) and interleukin (IL)-6 levels in BALF. Furthermore, IL-1beta level in BALF in the RPC group was significantly elevated at 24 hr after IT. Significant positive correlations were observed between blood neutrophil count and IL-6/G-CSF levels in BALF. MPO activity in the myocardium was increased by RPC at 12 and 24 hr after IT while the activities in the kidney and the liver were not affected. Significant correlation was also observed between myocardial MPO activity and blood neutrophil count at 12 hr after IT, for all three substances. From these results, it was concluded that particle component of DEP may enhance myocardial oxidative stress via blood neutrophilia and the elevation of cytokine levels in BALF.
Yokota
Syunji
S
First Laboratory of Toxicology, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan. yokota.s@fdsc.or.jp
Seki
Takayuki
T
Naito
Yukiko
Y
Tachibana
Shigehiro
S
Hirabayashi
Naoyuki
N
Nakasaka
Toshiaki
T
Ohara
Naoki
N
Kobayashi
Hiroyuki
H
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D019570:Inhalation Exposure / Q000009:adverse effects; D007668:Kidney / Q000187:drug effects / Q000276:immunology; D007958:Leukocyte Count; D008099:Liver / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D020556:Neutrophil Infiltration / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D018384:Oxidative Stress / Q000187:drug effects* / Q000276:immunology; D052638:Particulate Matter / Q000633:toxicity*; D009195:Peroxidase / Q000378:metabolism; D011014:Pneumonia / Q000097:blood / Q000139:chemically induced / Q000276:immunology; D014132:Trachea / Q000187:drug effects*; D001335:Vehicle Emissions / Q000633:toxicity*
D016207:Cytokines; D052638:Particulate Matter; D001335:Vehicle Emissions; D009195:Peroxidase