title: [Occurrence of AmpC-positive Klebsiella pneumoniae strains in patients with haematological malignancies].
abstract: BACKGROUND
  Currently, important bacterial beta-lactamases of increasing clinical significance include AmpC enzymes. The aim was to assess their occurrence in Klebsiella pneumoniae strains isolated from patients with haematological malignancies in a prospective study.
  
  
  METHODS
  Over a 2-month period, strains of the species were isolated from clinical material obtained from patients hospitalized at the Department of Haemato-Oncology of the University Hospital Olomouc. The strains were identified using standard microbiological techniques and the Vitek 2 automated system. Production of AmpC beta-lactamases was roughly determined by phenotypic tests and subsequently confirmed by PCR detection of genes encoding these enzymes.
  
  
  RESULTS
  During the above-mentioned period, a total of 35 K. pneumoniae isolates were collected. In 7 of them, production of AmpC beta-lactamases was preliminarily detected by phenotypic test. The multiplex PCR method confirmed phenotyping and determined DHA types in all the isolates.
  
  
  CONCLUSIONS
  All AmpC-positive isolates were false-susceptible to at least one of the tested third-generation cephalosporins. In one patient, clinically apparent infection caused by this strain was documented. The reported results suggest the possibility of occurrence of AmpC-beta-lactamases in K. pneumoniae strains with clinical significance.
authors.affiliation: Institute of Microbiology Faculty of Medicine UP Olomouc, Olomouc, Czech Republic. magdalena.chroma@centrum.cz
authors.forename: Magdaléna
authors.initials: M
authors.lastname: Chromá
authors.forename: Milan
authors.initials: M
authors.lastname: Kolár
authors.forename: Pavel
authors.initials: P
authors.lastname: Sauer
authors.forename: Edgar
authors.initials: E
authors.lastname: Faber
authors.forename: Tatána
authors.initials: T
authors.lastname: Stosová
authors.forename: Dagmar
authors.initials: D
authors.lastname: Koukalová
authors.forename: Karel
authors.initials: K
authors.lastname: Indrák
mesh_terms: D000208:Acute Disease; D001426:Bacterial Proteins / Q000378:metabolism*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000302:isolation & purification*; D007938:Leukemia / Q000382:microbiology*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases