title: Validation of plasma clearance of 51Cr-EDTA in adult renal transplant recipients: comparison with inulin renal clearance.
abstract: Plasma clearance of (51)Cr-EDTA ((51)Cr-EDTA-Cl) is an alternative method to evaluate glomerular filtration rate (GFR). This study aimed to investigate the concordance between (51)Cr-EDTA-Cl and renal inulin clearance (In-Cl) in renal transplant recipients as well to determine the repeatability of (51)Cr-EDTA-Cl in kidney donors. Forty four kidney recipients and 22 kidney donors were enrolled. Simultaneous measurements of (51)Cr-EDTA-Cl and In-Cl were performed. A single dose of 3.7MBq of (51)Cr-EDTA was injected and the plasma disappearance curve was created by taking blood samples at 2, 4, 6 and 8 h after injection. Bland and Altman statistical approach was used to quantify the agreement between In-Cl and (51)Cr-EDTA-Cl and to determine the better concordance between all possibilities of measure for the (51)Cr-EDTA-Cl. The mean of In-Cl was 44.5 +/- 17.9 ml/min/1.73 m(2). There was a positive correlation between In-Cl and all possible measurements of (51)Cr-EDTA-Cl. (51)Cr-EDTA-Cl with two samples taken at 4 and 8 h or at 4 and 6 h presenting the narrow limits of agreement and a difference (bias) of 2.8 and 2.7 ml/min, respectively. Two plasma sampling for (51)Cr-EDTA-Cl was a reliable method to measure GFR compared with In-Cl and comprises a suitable method to be used in kidney transplanted patients.
authors.affiliation: Division of Nephrology, School of Medicine, University of São Paulo, São Paulo, Brazil. fsreismedeiros@gmail.com
authors.forename: Flávia Silva Reis
authors.initials: FS
authors.lastname: Medeiros
authors.forename: Marcelo T
authors.initials: MT
authors.lastname: Sapienza
authors.forename: Elisângela S
authors.initials: ES
authors.lastname: Prado
authors.forename: Fabiana
authors.initials: F
authors.lastname: Agena
authors.forename: Maria H M
authors.initials: MH
authors.lastname: Shimizu
authors.forename: Francine B C
authors.initials: FB
authors.lastname: Lemos
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Buchpiguel
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Ianhez
authors.forename: Elias
authors.initials: E
authors.lastname: David-Neto
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000493:pharmacokinetics; D002860:Chromium Radioisotopes; D004492:Edetic Acid / Q000493:pharmacokinetics; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D007004:Hypoglycemic Agents / Q000493:pharmacokinetics; D007328:Insulin / Q000493:pharmacokinetics; D007677:Kidney Function Tests / Q000379:methods* / Q000592:standards*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D008954:Models, Biological
chemical_list: D000925:Anticoagulants; D002860:Chromium Radioisotopes; D007004:Hypoglycemic Agents; D007328:Insulin; D004492:Edetic Acid