Studies on the nature of hemolytic effect induced by ethylene glycol alkyl ethers.
The nature of hemolytic effect induced by ethylene glycol alkyl ethers was analyzed taking into account G-6-PDH activity, ATP, pyruvate and thiols levels in peripheral blood of rats treated with single doses of 2-ethoxyethanol and 2-butoxyethanol. In addition, the susceptibility to autoxidation of rat erythrocyte lipids was evaluated. A decrease of ATP level in a dose-dependent manner and an increase in protein- and nonprotein-bound sulfhydryl groups were observed. These results indicate that an acute hemolytic effect of ethylene glycol alkyl ethers is not associated with alterations in G-6-PDH activity and the susceptibility of erythrocyte lipids to autoxidation. Increases in protein- and nonprotein-bound sulfhydryl groups seem to indicate the selective hemolysis of the aged erythrocytes. The increase in pyruvate and thiol levels may protect erythrocytes against the appearance of oxidative stress.
Starek
Andrzej
A
Department of Biochemical Toxicology, Medical College, Jagiellonian University, 9 Medyczna Str. Kraków, Poland. mfstarek@cyf-kr.edu.pl
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005026:Ethylene Glycols / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
D005026:Ethylene Glycols; D008315:Malondialdehyde; C017096:n-butoxyethanol; C021296:2-ethoxyethanol