Cord blood-derived mesenchymal stem cell does not stimulate nor inhibits T lymphocytes activation.
The immune modulatory properties of mesenchymal stem cell (MSC) had brought a new insight in cell-based neotherapy. However, recent works of MSC are focused exclusively on bone marrow-derived MSC. We evaluated the immunogenicity of cord blood-derived MSC (CB-MSC) on T lymphocytes. Human peripheral blood mononuclear cells (PBMC) were prepared by density gradient separation and culture with the presence or absence of CB-MSC. PBMC were collected for activation analysis by flow cytometry at 24-, 48-, and 72- hours. The results showed that, CB-MSC does not stimulate nor inhibit T lymphocyte activation.
Tong
C K
CK
Department of Pathology, Faculty of Medicine and Health Sciences, University Putra, Malaysia.
Seow
H F
HF
Ramasamy
R
R
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D001853:Bone Marrow / Q000276:immunology*; D018929:Cell Culture Techniques; D005312:Fetal Blood / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007125:Immunogenetics; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D036102:Peripheral Blood Stem Cell Transplantation; D013601:T-Lymphocytes / Q000166:cytology*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000099192:CD69 Antigens