Biodegradation and saccharification of wood chips of Pinus strobus and Liriodendron tulipifera by white rot fungi.
Degradation and glucose production from wood chips of white pine (Pinus strobus) and tulip tree (Liriodendron tulipifera) by several white rot fungi were investigated. The highest weight losses from 4 g of wood chips of P. strobus and L. tulipifera by the fungal degradation on yeast extractmalt extract-glucose agar medium were 38% of Irpex lacteus and 93.7% of Trametes versicolor MrP 1 after 90 days, respectively. When 4 g of wood chips of P. strobus and L. tulipifera biodegraded for 30 days were treated with cellulase, glucose was recovered ot the highest values of 106 mg/g degraded wood by I. lacteus and 450 mg/g degraded wood by T. versicolor. The weight loss of 10 g of wood chip of L. tulipifera by T. versicolor on the nutrient non-added agar under the nonsterile conditions was 35% during 7 weeks of incubation, and the cumulative amount of glucose produced during this period was 239 mg without cellulase treatment. The activities of ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase) of fungi tested did not show a high correlation with degradation of the wood chips and subsequent glucose formation. These results suggest that the selection of proper wood species and fungal strain and optimization of glucose recovery are all necessary for the fungal pretreatment of woody biomass as a carbon substrate.
Hwang
Soon-Seok
SS
Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Lee
Sung-Jae
SJ
Kim
Hee Kyu
HK
Ka
Jong-Ok
JO
Kim
Kyu-Joong
KJ
Song
Hong-Gyu
HG
D001487:Basidiomycota / Q000201:enzymology / Q000378:metabolism; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D002480:Cellulase / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D031567:Liriodendron / Q000378:metabolism*; D028223:Pinus / Q000378:metabolism*; D055454:Trametes / Q000201:enzymology / Q000378:metabolism; D014934:Wood / Q000378:metabolism*
D003470:Culture Media; D008031:Lignin; D002480:Cellulase; D005947:Glucose