Regulation of CO2 fixation gene expression in Acidithiobacillus ferrooxidans ATCC 23270 by Lix984n shock.
Acidithiobacillus ferrooxidans ATCC 23270 is an important model organism for bioleaching and bioremediation studies owing to its diverse metabolic capabilities, whereas lix984n is a widely used extractant. Little is known about the response of cbb genes in A. ferrooxidans to lix984n shock. Thus, to elucidate the response of the CO2 fixation genes in A. ferrooxidans ATCC 23270 to the addition of lix984n, the gene expression of cbb genes was examined using a real-time PCR. Although a natural increase or decrease in the expression of most cbb genes was observed after 5 min of shock with 3% (v/v) lix984n, sdhC and cbbR exhibited quick responses to the shock. Ten min of shock had a greater effect on the cbb gene expression, yet 15 min of shock had a significant effect on the Calvin cycle in A. ferrooxidans ATCC 23270, as the expression of all the cbb genes reached a very high level. Therefore, after a short lix984n shock, a solution of A. ferrooxidans can be re-used for bioleaching.
Wang
Wei
W
School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, PR China.
Xiao
Shuiming
S
Chao
Jing
J
Chen
Qijiong
Q
Qiu
Guanzhou
G
Liu
Xueduan
X
D042763:Acidithiobacillus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002245:Carbon Dioxide / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010091:Oximes / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods
D001426:Bacterial Proteins; D010091:Oximes; D002245:Carbon Dioxide; D003576:Electron Transport Complex IV