First isolation of Neospora caninum from the faeces of a dog from Portugal.
We report the in vitro isolation of Neospora caninum from the faeces of a naturally infected 8-year-old male stray boxer from Portugal. Vero cell cultures were infected using parasite stages obtained after oral inoculation of gamma-interferon knockout mice with 10(2) sporulated oocysts. The isolate was identified by microscopical examination, as well as histological, immunological and molecular methods including a DNA-microsatellite-based typing technique, and was subsequently named NC-P1. The DNA-microsatellite pattern observed in the NC-P1 isolate was not previously reported for any N. caninum isolate. To our knowledge, this is the first isolation of N. caninum from the faeces of a naturally infected dog from Portugal.
Basso
W
W
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany. wbasso@fcv.unlp.edu.ar
Herrmann
D C
DC
Conraths
F J
FJ
Pantchev
N
N
Vrhovec
M Globokar
MG
Schares
G
G
D000818:Animals; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology*; D008297:Male; D018688:Neospora; D011174:Portugal / Q000453:epidemiology