Surface engineering of poly(D,L-lactic acid) by entrapment of soluble eggshell membrane protein.
Soluble eggshell membrane protein (SEP) is used to engineer poly(D,L-lactic acid) (PDL-LA) membrane surface by a physical entrapment method to enhance cytocompatibility. Attenuated total reflection Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and contact angle measurements are used to characterize SEP-modified PDL-LA membrane surface and confirm the formation of an even and stable layer of SEP on PDL-LA membrane surface. NIH3T3 cell lines are used as model cells to evaluate cytocompatibility of the SEP-modified PDL-LA membrane, which is much better than that of virgin PDL-LA membrane. The enhanced cytocompatibility facilitates potential application in tissue engineering.
Lu
Jian-Wei
JW
Department of Chemical Engineering, Institute of Polymer Science and Engineering, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Li
Qiang
Q
Qi
Qing-Lei
QL
Guo
Zhao-Xia
ZX
Yu
Jian
J
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D003432:Cross-Linking Reagents / Q000737:chemistry; D004528:Egg Shell; D005976:Glutaral / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005976:Glutaral