title: Development of sandwich enzyme-linked immunosorbent assay systems for plasma beta-galactoside alpha2,6-sialyltransferase, a possible hepatic disease biomarker.
abstract: Previous reports, including our work, have shown that plasma beta-galactoside alpha2,6-sialyltransferase (ST6Gal I) activity is significantly increased in particular hepatopathological situations, suggesting that it may represent a sensitive biomarker for diagnosing hepatic diseases. So far, activity of ST6Gal I have been measured by using radioactive tracer method in place of measuring amount of ST6Gal I. However, this method is tangled and cannot exclude other sialyltransferase activities. Thus, simple and specific methods for measuring plasma ST6Gal I had been unavailable. Here, we developed two kinds of sandwich enzyme-linked immunosorbent assay (ELISA) systems that specifically detect the soluble cleaved form of ST6Gal I in plasma. In one sandwich ELISA, we detected rat specific sequence, EFQMPK, which is N-terminus of soluble ST6Gal I. In the other sandwich ELISA, we detected internal common sequence among rat, mouse and human ST6Gal I in plasma (M2 ELISA). Using the M2 ELISA, we observed that elevation of plasma ST6Gal I was much faster than elevation of plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in a carbon tetrachloride (CCl(4))-induced mouse liver injury model. Our data suggest that these ELISA systems are very useful tools for measuring plasma ST6Gal I, which represents a potential biomarker for diagnosing hepatic diseases.
authors.affiliation: Department of Biochemistry, School of Medicine, Fukushima Medical University, 1 Hikarigaoka, Fukushima-shi, Fukushima 960-1295, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Futakawa
authors.forename: Shinobu
authors.initials: S
authors.lastname: Kitazume
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Oka
authors.forename: Kazuko
authors.initials: K
authors.lastname: Ogawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: Akinori
authors.initials: A
authors.lastname: Kinoshita
authors.forename: Kazuya
authors.initials: K
authors.lastname: Miyashita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hashimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D001774:Blood Chemical Analysis / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008107:Liver Diseases / Q000097:blood*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D012680:Sensitivity and Specificity; D012799:Sialyltransferases / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D012995:Solubility; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D000906:Antibodies; D015415:Biomarkers; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase