Characterization of the porcine differentially expressed PDK4 gene and association with meat quality.
To investigate the differential expression of genes in the skeletal muscle between Yorkshire and Chinese indigenous breed Meishan pigs, suppression subtractive hybridization was carried out and many genes were proved to be expressed significantly different in the two breeds. One gene highly expressed in Meishan but lowly expressed in Yorkshire specific library, shared strong homology with human pyruvate dehydrogenase kinase 4 (PDK4). Using semi-quantity and quantity PCR, We confirmed its differential expression between the two breeds. Temporal and spatial expression analysis indicated that porcine PDK4 gene is highly expressed in skeletal muscle and the highest in neonatal pigs. Complete cDNA cloning and sequence analysis revealed that porcine PDK4 gene contains an open reading frame of 1,221 bp. The deduced amino acid sequence showed conservation in evolution. A G/A mutation in intron 9 was identified and association analysis showed that it was significantly associated with intramuscular fat, muscle water content.
Lan
Jing
J
Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People's Republic of China.
Lei
Ming-Gang
MG
Zhang
Yi-Bing
YB
Wang
Jian-Hua
JH
Feng
Xiao-Ting
XT
Xu
De-Quan
DQ
Gui
Jian-Fang
JF
Xiong
Yuan-Zhu
YZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D007438:Introns / Q000235:genetics; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics*; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger; D011494:Protein Kinases; C403287:pyruvate dehydrogenase kinase 4