Atypical micromorphology and uncommon location of cryptococcosis: a histopathologic study using special histochemical techniques (one case report).
Here we report an unusual case of disseminated cryptococcosis in a patient with AIDS. Although typical Cryptococcus neoformans micromorphology was observed in tongue biopsy, cervical lymph node examination revealed atypical histopathologic findings. These included pseudohyphae, chains of budding yeasts and structures resembling germ tubes. Cryptococcus neoformans infection in supraclavicular lymph nodes was also confirmed by culture. The importance of using special histochemical techniques-Mayer's mucicarmine stain for mucicarminophilic capsule and Grocott's silver stain-in the diagnosis of cryptococcosis is reinforced.
Gazzoni
Alexandra Flávia
AF
Post-graduation Program in Pulmonary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. aleflavia@yahoo.com.br
Severo
Cecília Bittencourt
CB
Barra
Marines Bizarro
MB
Severo
Luiz Carlos
LC
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology*; D000328:Adult; D002329:Carmine; D003453:Cryptococcosis / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology*; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000472:pathogenicity* / Q000648:ultrastructure*; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D008297:Male; D009333:Neck; D016622:Silver Staining; D013194:Staining and Labeling; D014059:Tongue / Q000382:microbiology* / Q000473:pathology
C029618:mucicarmine; D002329:Carmine