title: Different epithelial cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis.
abstract: OBJECTIVE
  To determine the anatomic cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis (epi-LASIK).
  
  
  METHODS
  Department of Ophthalmology, IIsan Paik Hospital, Goyang, Korea.
  
  
  METHODS
  Sixteen eyes (8 patients) were included in this study. Three epikeratomes, the Moria Epi-K, Centurion SES, and Amadeus II, were used to collect 4 epithelial flaps from 2 patients in the epi-LASIK procedure. Four epithelial flaps from 2 patients were also obtained by laser-assisted subepithelial keratectomy (LASEK). Immunohistochemical staining using monoclonal antibodies against integrin beta1, integrin beta4, laminin 5, and collagen type VII was performed.
  
  
  RESULTS
  Immunohistochemical staining showed expression of integrin beta1 and integrin beta4 in all epithelial flaps. In epi-LASIK, the expression of laminin 5 and collagen type VII had a linear or dotted pattern that differed based on the epikeratome used. In the epithelial flaps obtained using LASEK, the expression of laminin 5 and collagen type VII had a dotted pattern.
  
  
  CONCLUSIONS
  Each epikeratome yielded reproducible but different cleavage planes of corneal epithelium. The results suggest that further study is needed to elucidate the wound-healing process after epi-LASIK because different cleavage planes produced by different epikeratomes may influence the process.
authors.affiliation: Department of Ophthalmology, Ilsan Paik Hospital, College of Medicine, Inje University, Ilsanseo-gu, Goyang, Korea.
authors.forename: Suk Kyue
authors.initials: SK
authors.lastname: Choi
authors.forename: Jin Hyoung
authors.initials: JH
authors.lastname: Kim
authors.forename: Doh
authors.initials: D
authors.lastname: Lee
authors.forename: Jae Bum
authors.initials: JB
authors.lastname: Lee
authors.forename: Hyo Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Hung Won
authors.initials: HW
authors.lastname: Tchah
authors.forename: Tae Won
authors.initials: TW
authors.lastname: Hahn
authors.forename: Mee
authors.initials: M
authors.lastname: Joo
authors.forename: Chang Il
authors.initials: CI
authors.lastname: Ha
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D024143:Collagen Type VII / Q000378:metabolism; D019573:Epithelium, Corneal / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019012:Integrin beta1 / Q000378:metabolism; D039663:Integrin beta4 / Q000378:metabolism; D049008:Keratectomy, Subepithelial, Laser-Assisted / Q000379:methods*; D020731:Keratomileusis, Laser In Situ / Q000379:methods*; D054018:Lasers, Excimer / Q000627:therapeutic use*; D008297:Male; D009216:Myopia / Q000601:surgery; D013524:Surgical Flaps / Q000473:pathology*; D014945:Wound Healing; D000097794:Kalinin
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D024143:Collagen Type VII; D019012:Integrin beta1; D039663:Integrin beta4; D000097794:Kalinin