title: Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules.
abstract: During mitosis, sister chromatids congress to the spindle equator and are subsequently segregated via attachment to dynamic kinetochore microtubule (kMT) plus ends. A major question is how kMT plus-end assembly is spatially regulated to achieve chromosome congression. Here we find in budding yeast that the widely conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kMTs. Of the two, Cin8p is the major effector and its activity requires a functional motor domain. In contrast, the depolymerizing kinesin-8 motor Kip3p plays a minor role in spatial regulation of yeast kMT assembly. Our analysis identified a model where kinesin-5 motors bind to kMTs, move to kMT plus ends, and upon arrival at a growing plus end promote net kMT plus-end disassembly. In conclusion, we find that length-dependent control of net kMT assembly by kinesin-5 motors yields a simple and stable self-organizing mechanism for chromosome congression.
authors.affiliation: Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Melissa K
authors.initials: MK
authors.lastname: Gardner
authors.forename: David C
authors.initials: DC
authors.lastname: Bouck
authors.forename: Leocadia V
authors.initials: LV
authors.lastname: Paliulis
authors.forename: Janet B
authors.initials: JB
authors.lastname: Meehl
authors.forename: Eileen T
authors.initials: ET
authors.lastname: O'Toole
authors.forename: Julian
authors.initials: J
authors.lastname: Haase
authors.forename: Adelheid
authors.initials: A
authors.lastname: Soubry
authors.forename: Ajit P
authors.initials: AP
authors.lastname: Joglekar
authors.forename: Mark
authors.initials: M
authors.lastname: Winey
authors.forename: Edward D
authors.initials: ED
authors.lastname: Salmon
authors.forename: Kerry
authors.initials: K
authors.lastname: Bloom
authors.forename: David J
authors.initials: DJ
authors.lastname: Odde
mesh_terms: D015825:Chromosomes, Fungal / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism*; D018386:Kinetochores / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D020409:Molecular Motor Proteins; D009154:Mutation; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C075342:CIN8 protein, S cerevisiae; C431747:KIP1 protein, S cerevisiae; C108256:KIP3 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D016547:Kinesins