title: [Association between polycyclic aromatic hydrocarbons exposure levels and nucleoplasmic bridge and nuclear bud frequencies in coke-oven workers].
abstract: OBJECTIVE
  To seek new effect biomarkers as to evaluating the chromosomal damage in peripheral blood lymphocytes in coke-oven workers who were exposed to polycyclic aromatic hydrocarbons (PAHs).
  
  
  METHODS
  One hundred and fifty-eight coke-oven workers and 69 controls were recruited in this study. Nucleoplasmic bridges and nuclear buds were counted as indicators of chromosomal damage in terms of cytokinesis-block micronucleus (CBMN) test. Occupational history, age, sex, smoking and alcohol using status of all subjects were collected by questionnaire.
  
  
  RESULTS
  Frequencies of nucleoplasmic bridge in coke-oven workers were (9.41 +/- 3.73)% per hundred, and the frequencies of nuclear buds were (7.13 +/- 4.01)% per hundred, which were significantly higher (P < 0.01) than those of controls (1.88 +/- 1.49)% per hundred and (2.20 +/- 1.73)% per hundred respectively. The dose-effect relationships between nucleoplasmic bridges or nuclear buds and PAHs exposure levels were identified. Compared with male coke-oven workers, female workers had less nucleoplasmic bridges or nuclear buds. No effects of age, smoking and alcohol using were found on nucleoplasmic bridges or nuclear buds among coke-oven workers.
  
  
  CONCLUSIONS
  Nucleoplasmic bridges and nuclear buds might be effect biomarkers in coke-oven workers.
authors.affiliation: National Institute of Occupational Health Poison Control, Chinese Center for Disease Control Prevention, Beijing 100050, China.
authors.forename: Hua-wei
authors.initials: HW
authors.lastname: Duan
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Leng
authors.forename: Zu-fei
authors.initials: ZF
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Ping
authors.initials: P
authors.lastname: Bin
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000428:Alcohol Drinking; D002467:Cell Nucleus; D003077:Coke; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes; D008297:Male; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D012907:Smoking
chemical_list: D003077:Coke; D011084:Polycyclic Aromatic Hydrocarbons