title: Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi.
abstract: In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb0665 and bb0667) are cotranscribed with the motility and chemotaxis genes. This newly identified operon is termed as pami. Sequence and primer extension analyses showed that pami was regulated by a sigma(70)-like promoter, which is designated as P(ami). Transcriptional analysis using a gene encoding green fluorescence protein as a reporter demonstrated that P(ami) functions in both Escherichia coli and B. burgdorferi. Genetic studies showed that the Deltabb0666 mutant grows in long chains of unseparated cells, whose phenotype is similar to its counterparts in E. coli. Taken together, these results demonstrate that bb0666 is a homolog of MurNac-LAAs that contributes to the cell division of B. burgdorferi.
authors.affiliation: Department of Oral Biology, State University of New York at Buffalo, NY 14214-3092, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Chunhao
authors.initials: C
authors.lastname: Li
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D025065:Borrelia burgdorferi / Q000737:chemistry / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D002455:Cell Division; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D000581:Amidohydrolases; C019665:amidase