Unprecedented biological cyclopropanation in the biosynthesis of FR-900848.
We were able to show the predominant incorporation of a single enantiomer and intact incorporation of multiply labelled synthetic diketide precursors (and), which established the intermediacy of cyclopropanated diketide and led to our proposal for the unprecedented biological cyclopropation, via PKS (polyketide synthase) having a novel cyclopropanase domain, in the biosynthesis of FR-900848 (1).
Tokiwano
Tetsuo
T
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, 010-0195, Japan. tkwn@akita-pu.ac.jp
Watanabe
Hiroaki
H
Seo
Takashi
T
Oikawa
Hideaki
H
D039903:Actinobacteria / Q000201:enzymology; D000214:Acyl Coenzyme A / Q000378:metabolism; D003521:Cyclopropanes / Q000494:pharmacology; D008954:Models, Biological; D009705:Nucleosides / Q000096:biosynthesis / Q000494:pharmacology; D048630:Polyketide Synthases / Q000378:metabolism; D013237:Stereoisomerism
D000214:Acyl Coenzyme A; D003521:Cyclopropanes; D009705:Nucleosides; C065437:FR 900848; D048630:Polyketide Synthases