title: Fluorescent labeling of peptides on solid phase.
abstract: N(alpha)-Fmoc-N(epsilon)-[(7-methoxycoumarin-4-yl)acetyl]-L-lysine (N(alpha)-Fmoc-L-Lys(Mca)-OH) 3 is conveniently prepared by benzotriazole methodology (52% over two steps). N-Acylbenzotriazoles Mca-Bt 2, N(alpha)-Fmoc-L-Lys(Mca)-Bt 4, coumarin-3-ylcarbonyl (Cc)-Bt 5, N(alpha)-Fmoc-L-Lys(Cc)-Bt 7 and N(alpha)-(Cc)-L-Lys(Fmoc)-Bt 9 enable the efficient microwave enhanced solid-phase fluorescent labeling of peptides.
authors.affiliation: University of Florida, Gainesville, FL 32611-7200, USA. katritzky@chem.ufl.edu
authors.forename: Alan R
authors.initials: AR
authors.lastname: Katritzky
authors.forename: Megumi
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Tamari
authors.initials: T
authors.lastname: Narindoshvili
authors.forename: Alfred
authors.initials: A
authors.lastname: Chung
authors.forename: Jodie V
authors.initials: JV
authors.lastname: Johnson
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000215:Acylation; D002264:Carboxylic Acids / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D013194:Staining and Labeling; D014230:Triazoles / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D003374:Coumarins; D005456:Fluorescent Dyes; D010455:Peptides; D014230:Triazoles; C030123:coumarin; D008239:Lysine; D019342:Acetic Acid