title: An investigation on the antibacterial and antibiofilm efficacy of cationic nanoparticulates for root canal disinfection.
abstract: This study aimed to investigate the antibacterial and antibiofilm efficacy of cationic nanoparticulates for root canal disinfection. Experiments were performed in two stages. In stage 1, experiments were conducted to examine the physical properties of three types of nanoparticulates. The antibacterial properties of nanoparticulates alone and nanoparticulates mixed with zinc oxide-eugenol-based sealer were studied. In stage 2, the ability of nanoparticulates-treated dentin to prevent bacterial adherence was examined. Zinc oxide nanoparticulates, chitosan nanoparticulates, a mixture of zinc oxide and chitosan nanoparticulates, and zinc oxide nanoparticulates with multilayered coating of chitosan were tested. This study showed that the incorporation of nanoparticulates did not alter the flow characteristics of sealer but improved the direct antibacterial property and the ability to leach out antibacterial components. There was a significant reduction in the adherence of Enterococcus faecalis to nanoparticulates-treated dentin (p < 0.05). These experiments highlighted the potential advantage of nanoparticulates in root canal disinfection.
authors.affiliation: Department of Restorative Dentistry, National University of Singapore, Singapore. rsdak@nus.edu.sg
authors.forename: Anil
authors.initials: A
authors.lastname: Kishen
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Shi
authors.forename: Annie
authors.initials: A
authors.lastname: Shrestha
authors.forename: Koon Gee
authors.initials: KG
authors.lastname: Neoh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001422:Bacterial Adhesion / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology; D015169:Colony Count, Microbial; D019551:Dental Disinfectants / Q000494:pharmacology*; D003786:Dental Pulp Cavity / Q000382:microbiology; D003804:Dentin / Q000382:microbiology; D013293:Enterococcus faecalis / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D012212:Rheology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D055672:Static Electricity; D013499:Surface Properties; D015034:Zinc Oxide / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D019551:Dental Disinfectants; D012388:Root Canal Irrigants; D015035:Zinc Oxide-Eugenol Cement; D048271:Chitosan; D015034:Zinc Oxide