[Role of phospholipase C-gamma1 signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells].
OBJECTIVE
To explore the role of phospholipase C-gamma1 (PLC-gamma1) signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells.


METHODS
PC12 cells were exposed to 50 micromol/L H(2)O(2) after pretreatment with 10 micromol/L U73122, a specific PLC-gamma1 inhibitor. Hoechst/PI double staining was performed to observe the morphological changes of the cells under light microscope. MTT assay was used to evaluate the cell viability, and the percentage of apoptotic cells was analyzed by flow cytometry. DNA fragmentation assay was carried out to characterize the cell apoptosis.


RESULTS
After inhibition of the PLC-gamma1 signaling pathway with 10 micromol/L U73122, PC12 cells showed obvious apoptotic morphology, the viable cells decreased significantly, and the percentage of apoptotic cells rose to 35.7%. PC12 cells treated with U73122 presented with a distinct DNA ladder on electrophoresis resulting from DNA cleavage in the apoptotic cells.


CONCLUSIONS
PLC-gamma1 signaling pathway plays an important protective role in H(2)O(2)-induced PC12 cell apoptosis.
Yuan
Wen-Li
WL
Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
Lu
Di
D
Sun
Jun
J
Chen
Guang-Xue
GX
Chen
Hui
H
Wang
Ting-Hua
TH
Luo
Shen-Qiu
SQ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004963:Estrenes / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016716:PC12 Cells; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011760:Pyrrolidinones / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction
D004963:Estrenes; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D006861:Hydrogen Peroxide; D051966:Phospholipase C gamma