Cell attachment study of chicken fibroblast cell (DF-1) using ceramic microcarrier granule in bioreactors.
Tricalcium phosphate ceramic microcarrier has been developed and introduced to a new possibility for the culture of anchorage dependent animal cells of DF1. It was observed that the number of attached cells was increased with shorter time for both spinner vessel and stirred tank (ST) bioreactor. For those bioreactors, the total viable cell number that had been obtained is about 1.2 x 10(5) cell/ml.
Mel
M
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Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia.
Sopyan
I
I
Nor
Y Ahmad
YA
D000818:Animals; D001672:Biocompatible Materials; D019149:Bioreactors; D002130:Calcium Phosphates; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival; D002645:Chickens; D005347:Fibroblasts; D013997:Time Factors
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C018392:tricalcium phosphate