[Isolation, culture and purification of olfactory ensheathing cells from human fetal olfactory mucosa].
OBJECTIVE
To explore the method for obtaining olfactory ensheathing cells from human fetal olfactory mucosa by cell culture for selective adhesion in the presence of neurotrophin-3 (NT3) and low-concentration serum.


METHODS
The olfactory ensheathing cells were cultured alternatively in DMEM/F12 culture medium containing 10% fetal bovine serum (FBS) and the medium containing NT3 and 2.5% FBS every 72 h. The cells were observed for morphological changes and identified using immunocytochemistry with P75NTR and GFAP, and the cell purity was estimated.


RESULTS
The olfactory ensheathing cells from human fetal olfactory mucosa were positive for P75(NTR) and GFAP, and in in vitro culture, the cells exhibited dipolar or tripolar appearance with long thin neurites. On the 9th day of cell culture, the purity of the olfactory ensheathing cells reached about 83%.


CONCLUSIONS
The olfactory ensheathing cells can be obtained by in vitro culture for selective adhesion in the presence of NT3 and low-concentration serum.
Li
Qiang
Q
Department of Orthopedics, Oigdao Municipat Hospitat, Qingdao 266071, China.
He
Xi-Jing
XJ
Shi
Jian-Feng
JF
Wang
Bin
B
Zhu
Zhen-Zhong
ZZ
Zang
Quan-Jin
QJ
Fan
Pei
P
D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D006801:Humans; D020933:Neurotrophin 3 / Q000494:pharmacology; D009830:Olfactory Bulb / Q000166:cytology*; D009831:Olfactory Mucosa / Q000166:cytology*
D003470:Culture Media; D020933:Neurotrophin 3