Molecular cloning of Kazal-type proteinase inhibitor of the shrimp Fenneropenaeus chinensis.
Proteinase inhibitors play important roles in host defence systems involving blood coagulation and pathogen digestion. We isolated and characterized a cDNA clone for a Kazal-type proteinase inhibitor (KPI) from a hemocyte cDNA library of the oriental white shrimp Fenneropenaeus chinensis. The KPI gene consists of three exons and two introns. KPI cDNA contains an open reading frame of 396 bp, a polyadenylation signal sequence AATAAA, and a poly (A) tail. KPI cDNA encodes a polypeptide of 131 amino acids with a putative signal peptide of 21 amino acids. The deduced amino acid sequence of KPI contains two homologous Kazal domains, each with six conserved cysteine residues. The mRNA of KPI is expressed in the hemocytes of healthy shrimp, and the higher expression of KPI transcript is observed in shrimp infected with the white spot syndrome virus (WSSV), suggesting a potential role for KPI in host defence mechanisms.
Kong
Hee Jeong
HJ
Biotechnology Research Institute, National Fisheries Research and Development Institute, Gijang-gun, Busan, South Korea. hjkong@nfrdi.re.kr
Cho
Hyun Kook
HK
Park
Eun-Mi
EM
Hong
Gyeong-Eun
GE
Kim
Young-Ok
YO
Nam
Bo-Hye
BH
Kim
Woo-Jin
WJ
Lee
Sang-Jun
SJ
Han
Hyon Sob
HS
Jang
In-Kwon
IK
Lee
Chang Hoon
CH
Cheong
Jaehun
J
Choi
Tae-Jin
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D015842:Serine Proteinase Inhibitors