title: In vitro femtosecond laser-assisted nanosurgery of porcine posterior capsule.
abstract: OBJECTIVE
  To investigate femtosecond laser-assisted nanosurgery of the posterior capsule in a prospective in vitro animal study.
  
  
  METHODS
  Department of Ophthalmology and Eye Hospital, University of Saarland, Homburg, and Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany.
  
  
  METHODS
  The posterior capsules of 12 porcine eyes were irradiated with a nonamplified 90 MHz near-infrared 750 nm titanium:sapphire femtosecond laser. Intratissue and superficial laser cuts of laser-ablated (5 capsules) and control (1 capsule) specimens were examined by femtosecond multiphoton laser scanning microscopy (MLSM) and transmission electron microscopy (TEM).
  
  
  RESULTS
  Laser exposure time and pulse power determined the width of the lesions, which ranged from 0.69 microm+/-0.19 (SD) to 2.81+/-0.5 microm. Both MLSM and TEM revealed minimal collateral alterations in the tissue surrounding the laser cuts.
  
  
  CONCLUSIONS
  Nonamplified near-infrared femtosecond laser pulses at low pulse energies may be a promising strategy for precise lamellar noncontact nanosurgery of the posterior capsule, with minimal structural collateral damage to surrounding tissue. High-resolution MLSM offers 3-dimensional, noninvasive, nondestructive imaging at submicrometer resolution within seconds before and after ablation.
authors.affiliation: Department of Ophthalmology and Eye Hospital, University of Saarland, Homburg/Saar, Germany. doctorop@mail.ru
authors.forename: Sergey G
authors.initials: SG
authors.lastname: Toropygin
authors.forename: Matthias
authors.initials: M
authors.lastname: Krause
authors.forename: Iris
authors.initials: I
authors.lastname: Riemann
authors.forename: Berthold
authors.initials: B
authors.lastname: Seitz
authors.forename: Pedro
authors.initials: P
authors.lastname: Mestres
authors.forename: Klaus W
authors.initials: KW
authors.lastname: Ruprecht
authors.forename: Karsten
authors.initials: K
authors.lastname: König
mesh_terms: D000818:Animals; D053685:Laser Therapy / Q000379:methods*; D053844:Lasers, Solid-State; D007903:Lens Capsule, Crystalline / Q000601:surgery* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010865:Pilot Projects; D011446:Prospective Studies; D013552:Swine