Human embryonic stem cells reveal recurrent genomic instability at 20q11.21.
By analyzing five human embryonic stem (hES) cell lines over long-term culture, we identified a recurrent genomic instability in the human genome. An amplification of 2.5-4.6 Mb at 20q11.21, encompassing approximately 23 genes in common, was detected in four cell lines of different origins. This amplification, which has been associated with oncogenic transformation, may provide a selective advantage to hES cells in culture.
Lefort
Nathalie
N
INSERM/UEVE UMR-861, I-STEM, AFM, Institute for Stem cell Therapy and Exploration of Monogenic diseases, 5 rue Henri Desbruères, 91030 Evry cedex, France.
Feyeux
Maxime
M
Bas
Cécile
C
Féraud
Olivier
O
Bennaceur-Griscelli
Annelise
A
Tachdjian
Gerard
G
Peschanski
Marc
M
Perrier
Anselme L
AL
D002460:Cell Line; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D053595:Embryonic Stem Cells / Q000166:cytology*; D005784:Gene Amplification; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D012008:Recurrence