Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein.
We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in <or=24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and cheap, allowing for their routine use in clinical laboratories.
Ivanov
Iuri B
IB
Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Orenburg 460056, Russia. mil-for@rambler.ru
Gritsenko
Viktor A
VA
D001426:Bacterial Proteins / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D001792:Blood Platelets / Q000378:metabolism; D002908:Chronic Disease; D006801:Humans; D008297:Male; D011467:Prostate / Q000382:microbiology; D011472:Prostatitis / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000378:metabolism*; D001620:beta-Thromboglobulin / Q000037:antagonists & inhibitors*
D001426:Bacterial Proteins; C061615:PPBP protein, human; D001620:beta-Thromboglobulin