Effects of antikeratin 16 antibodies on the expression of Toll-like receptors 2 and 4 in keratinocytes.
Innate immunity plays an important role in psoriasis. The possible role of Toll-like receptors (TLRs) in the activation of innate immunity in psoriasis remains unknown. Serum antikeratin 16 (anti-K16) autoantibody levels are raised in patients with psoriasis. The aim of this study was to investigate the effects of anti-K16 antibodies on TLR2 and TLR4 expression in keratinocytes. Keratinocytes were incubated with mouse anti-K16 monoclonal antibodies. Levels of TLR2 and TLR4 were measured by quantitative real-time PCR and flow cytometry. TLR2 mRNA level was increased by 1.73-fold, 1.60-fold and 2.52-fold at 6, 24 and 36 h after incubation, respectively. TLR4 mRNA level was increased by 3.62-fold and 2.21-fold at 12 and 36 h, respectively. TLR2 protein expression was increased by 1.73-fold on the cell membrane and 2.22-fold in cell membrane and intracytoplasmic locations. There was no change in TLR4. The results of this study indicate that anti-K16 antibodies may be a modulator of TLRs.
Wu
C
C
Department of Dermatology, Huashan Hospital, Medical College, Fudan University, Shanghai, China.
Luan
Q
Q
Li
C
C
Zheng
Z
Z
D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D007113:Immunity, Innate; D053536:Keratin-16 / Q000276:immunology*; D015603:Keratinocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D011565:Psoriasis / Q000276:immunology*; D012867:Skin / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
D001323:Autoantibodies; D053536:Keratin-16; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4