Surface modification of hydrogels based on poly(2-hydroxyethyl methacrylate) with extracellular matrix proteins.
Infrared attenuated total reflection spectroscopy was used for in situ observation of the deposition of collagen I on poly(2-hydroxyethyl methacrylate-co-methacrylic acid, 2.9%) hydrogels and subsequent attachment of laminin or fibronectin on the collagen surface. While there was no adsorption of collagen dissolved in an acid solution on the hydrogel surface, it deposited on the surface at pH 6.5. The collagen layers with attached laminin or fibronectin were stable on hydrogel surface in physiological solution. The modification with collagen and particularly with collagen and laminin or fibronectin allowed the adhesion and growth of mesenchymal stromal cells and astrocytes on the hydrogel surface.
Brynda
Eduard
E
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic. brynda@imc.cas.cz
Houska
Milan
M
Kysilka
Jirí
J
Prádný
Martin
M
Lesný
Petr
P
Jendelová
Pavla
P
Michálek
Jirí
J
Syková
Eva
E
D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D016326:Extracellular Matrix Proteins; D020100:Hydrogels; C000257:polyhydroxyethyl methacrylate hydrogels; D011102:Polyhydroxyethyl Methacrylate