title: [Hepatocellular glycogen alleviates hepatic ischemia reperfusion injury and its relationship to ICAM-1 gene expression].
abstract: OBJECTIVE
  To investigate if higher hepatocellular glycogen contents can alleviate hepatic ischemia reperfusion injury and its relationship to ICAM-1 gene expression in hepatic sinusoidal cells (HSCs).
  
  
  METHODS
  Twenty-one rabbits fed with a standard diet were randomly divided into three groups (n=7 in each). All the animals were subjected to hepatic ischemia reperfusion injury then sacrificed. Before the injury, group A rabbits fasted for 24 hours; group C rabbits had 6 intravenous glucose solution (25%, 20 ml) injections, 4 hours between two injections. Hepatic enzymological changes, hepatic ICAM-1 mRNA expressions and leukocytic counts in the sinusoids were observed.
  
  
  RESULTS
  The liver glycogen contents of the three groups were significantly different. Livers of group A had higher contents of glycogen (9.85+/-0.91 mg/g. wet tissue); in group B they were 38.93+/-5.72; and in group C they were 48.31+/-6.58. Group C animals had the slightest liver function damage. There were no differences in the pre- and post-ischemic ICAM-1 mRNA contents in the three groups. However, livers with a higher content of glycogen showed less expression of ICAM-1 mRNA (group A: 1.398+/-0.365 ng/mg wet tissue; group B: 0.852+/-0.297; group C: 0.366+/-0.183) and lower leukocytic counts. The relationship analysis showed a negative relationship between hepatocellular glycogen and hepatic ICAM-1 mRNA contents (r= -0.965, P less than 0.01).
  
  
  CONCLUSIONS
  Hepatocellular glycogen is important in protecting liver ischemic reperfusion injury. Also hepatocellular glycogen decreases the expression of ICAM-1 mRNA of HSCs.
authors.affiliation: Center of General Surgery of PLA, PLA Chengdu General Hospital, Chengdu 610083, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Tang
authors.forename: Fu-zhou
authors.initials: FZ
authors.lastname: Tian
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Cui
authors.forename: Hao
authors.initials: H
authors.lastname: Luo
authors.forename: Dong-xuan
authors.initials: DX
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Shu
authors.initials: S
authors.lastname: Zou
mesh_terms: D000818:Animals; D005260:Female; D006003:Glycogen / Q000494:pharmacology*; D022781:Hepatocytes / Q000737:chemistry / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D006003:Glycogen