title: [Recombinant envelope glycoprotein domain III of dengue virus inhibit virus infection].
abstract: OBJECTIVE
  To observe the ability of dengue virus recombinant envelope protein domain expressed in E. coli to inhibit virus infection and induce the neutralizing antibody.
  
  
  METHODS
  E III protein of Dengue virus serotypes 1-4 were expressed in E. coli BL21(DE3) then purified. Recombinant proteins were tested to inhibit DV2 from infecting BHK-21 cell. Rabbits were immunized with recombinant proteins to produce anti-E III serum. Antibody titers were determined by neutralizing assay.
  
  
  RESULTS
  The recombinant E III proteins of Dengue virus serotypes 1-4 were expressed in E. coli. They effectively protected BHK cells in culture against DV2 infection. All four type anti-E III sera can neutralize DV2 but their efficacies are different.
  
  
  CONCLUSIONS
  proteins of dengue virus expressed in E. coli can directly inhibit DV2 infection. Neutralizing antibodies were induced by E III proteins. Both E III protein of dengue virus and the neutralizing antibodies they induced are more efficient in inhibiting homologous dengue serotypes infection than heterologous serotypes.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Shou-Chun
authors.initials: SC
authors.lastname: Cao
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Li
authors.forename: Qin-Zhi
authors.initials: QZ
authors.lastname: Liu
authors.forename: Quan-Fu
authors.initials: QF
authors.lastname: Zhang
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Miao
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Hang
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Liang
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015536:Down-Regulation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007114:Immunization; D008647:Mesocricetus; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins