[Determination of plasma escitalopram with liquid chromatography-mass spectrometry].
OBJECTIVE
To establish an liquid chromatography-mass spectrometry (HPLC-MS/MS)-based method for determining escitalopram in human plasma.


METHODS
A liquid-liquid ether extraction approach was adopted using dextromethorphan as the internal standard. The Agilent ZORBAX SB-C18 (3.5 microm,2.1 x 150 mm) was used as the analytical column with acetonitrile-NH4AC buffer (70:30, V/V) as the mobile phase at the flow rate of 0.3 ml/min. The sample was ionized by electrospray ionization source in the triple quadruple tandem mass spectrometer, and the plasma escitalopram determined with a multiple reaction monitoring mode of m/z325.4-->109.4.


RESULTS
The linear range was 0.0866-64.13 microg/L (r=0.9965) for escitalopram in human plasma, with the absolute recovery between 64.98% and 78.72% and the within-day and between-day deviations less than 10%.


CONCLUSIONS
The method is sensitive, accurate, rapid, specific and well applicable for clinical pharmacokinetics study of escitalopram.
Zheng
Heng
H
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. hzheng@tjh.tjmu.edu.cn
Yang
Ling
L
Tang
Wen-Yan
WY
D018687:Antidepressive Agents, Second-Generation / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015283:Citalopram / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D018687:Antidepressive Agents, Second-Generation; D015283:Citalopram