title: Development of human Graafian follicles following transplantation of human ovarian tissue into NOD/SCID/gammac null mice.
abstract: OBJECTIVE
  Transplantation of human ovarian cortex into host mice may permit various kinds of challenges in reproductive medicine. A novel immunodeficient mouse strain (NOD/SCID/gammacnull: NOG) has been developed as a host of transplantation of human tissue.
  
  
  METHODS
  Human ovarian cortex was transplanted into various sites of NOG mice and human follicular development was examined by immunohistochemistry.
  
  
  RESULTS
  Transplantation of human ovarian tissue into NOG mice resulted in approximately similar tissue survival and follicle growth as did transplantation into non-obese diabetic-severe combined immunodeficient mice. The human Graafian follicle from NOG mouse expressed the same steroidogenic enzymes as observed in human Graafian follicles, which developed in the human body. The NOG mice's ovarian bursa was better placed for transplantation than the back skin or kidney capsule.
  
  
  CONCLUSIONS
  These results represent the successful generation and biological confirmation of the human Graafian follicles from the human ovarian cortex in the NOG mice.
authors.affiliation: Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. terada@mail.tains.tohoku.ac.jp
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Terada
authors.forename: Yumi
authors.initials: Y
authors.lastname: Terunuma-Sato
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kakoi-Yoshimoto
authors.forename: Hisataka
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ugajin
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
authors.forename: Hironobu
authors.initials: H
authors.lastname: Sasano
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D053631:Interleukin Receptor Common gamma Subunit / Q000172:deficiency* / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000276:immunology*; D016042:Transplantation, Heterotopic; D055815:Young Adult
chemical_list: C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit