[Determination of the activity of semicarbazide-sensitive amine oxidase in the differentiated 3T3-F442A cells by enzyme-linked spectrophotometric assay].
In order to probe into the patho-physiology semicarbazide-sensitive amine oxidase (SSAO), we determined the variation of its activity in the days of 3T3-F442A cells' differentiation into adipocytes in vitro. The differentiated 3T3-F442A cells were collected in various days and disrupted by ultrasonication. Then we studied the SSAO activity of the collected cells by oxidase-linked spectrophotometric assay. In the course of 3T3-F442A cells' differentiation, SSAO expression was shown by a curve graph. There was no SSAO detected in 3T3-F442A preadipocyte, and only a little of SSAO was detected in the cells on the 3rd day of differentiation. Since then, SSAO significantly increased and reached to a maximum level six or seven days after the cells' differentiation.
Zhu
Sha
S
Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China. zsha@zzu.edu.cn
Wang
Yan
Y
Du
Ying
Y
Li
Sanqiang
S
Xuan
Xiaoyan
X
Tang
Yue
Y
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology*; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D051379:Mice; D013053:Spectrophotometry / Q000379:methods*
D006631:Amine Oxidase (Copper-Containing)