title: Simple and sensitive method for quantification of fluorescent enzymatic mature and senescent crosslinks of collagen in bone hydrolysate using single-column high performance liquid chromatography.
abstract: A rapid high performance liquid chromatographic method was developed including an internal standard for the measurement of mature and senescent crosslinks concentration in non-demineralized bone hydrolysates. To avoid the demineralization which is a tedious step, we developed a method based on the use of a solid-phase extraction procedure to clean-up the samples. It resulted in sensitive and accurate measurements: the detection limits as low as 0.2 pmol for the pyridimium crosslinks and 0.02 pmol for the pentosidine. The inter- and intra-assay coefficients of variation were as low as 5% and 2%, respectively, for all crosslinks.
authors.affiliation: INSERM Research Unit 831, Claude Bernard University of Lyon, Lyon, France.
authors.forename: S
authors.initials: S
authors.lastname: Viguet-Carrin
authors.forename: E
authors.initials: E
authors.lastname: Gineyts
authors.forename: C
authors.initials: C
authors.lastname: Bertholon
authors.forename: P D
authors.initials: PD
authors.lastname: Delmas
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000737:chemistry*; D005453:Fluorescence; D006868:Hydrolysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003094:Collagen