Inhibition of SNARE-driven neuroexocytosis by plant extracts.
Neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins mediate membrane fusion between synaptic vesicle and presynaptic membrane, resulting in neurotransmitter release. SNARE proteins are specific substrates of botulinum neurotoxins (BoNT) which are now widely used for therapeutic and cosmetic purposes. While BoNT blocks neuroexocytosis by cleaving SNAREs, inhibiting SNARE assembly process might exert the same effect on neurotransmission. In the present study, some extracts of 100 plants reduced neurotransmitter release by inhibiting SNARE complex formation in neuronal cells. The extracts effectively paralyzed muscle of rat phrenic nerve-hemidiaphragm preparation. Our results raise the possibility that SNARE folding inhibitors from natural resources might replace some special BoNT application fields.
Jung
Chang Hwa
CH
School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, 440-746, South Korea.
Yang
Yoo Soo
YS
Kim
Jun-Seob
JS
Shin
Yeon-Kyun
YK
Hwang
Jae Sung
JS
Son
Eui Dong
ED
Lee
Hong Hwa
HH
Chung
Koo Min
KM
Oh
Jung Mi
JM
Lee
Jong Hwa
JH
Kweon
Dae-Hyuk
DH
D000818:Animals; D003964:Diaphragm / Q000187:drug effects; D005089:Exocytosis / Q000187:drug effects*; D009466:Neuromuscular Blocking Agents / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D010243:Paralysis; D010791:Phrenic Nerve / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D050600:SNARE Proteins / Q000037:antagonists & inhibitors*; D009435:Synaptic Transmission / Q000187:drug effects*
D009466:Neuromuscular Blocking Agents; D010936:Plant Extracts; D050600:SNARE Proteins