A comparative proteome approach to decipher the mechanism of rice adaptation to phosphorous deficiency.
Mineral deficiency limits crop production in most soils and in Asia alone, about 50% of rice lands are phosphorous deficient. In an attempt to determine the mechanism of rice adaptation to phosphorous deficiency, changes in proteome patterns associated with phosphorous deficiency have been investigated. We analyzed the parental line Nipponbare in comparison to its near isogenic line (NIL6-4) carrying a major phosphorous uptake QTL (Pup1) on chromosome 12. Using 2-DE, the proteome pattern of roots grown under 1 and 100 microM phosphorous were compared. Out of 669 proteins reproducibly detected on root 2-DE gels, 32 proteins showed significant changes in the two genotypes. Of them, 17 proteins showed different responses in two genotypes under stress condition. MS resulted in identification of 26 proteins involved in major phosphorous deficiency adaptation pathways including reactive oxygen scavenging, citric acid cycle, signal transduction, and plant defense responses as well as proteins with unknown function. Our results highlighted a coordinated response in NIL in response to phosphorous deficiency which may confer higher adaptation to nutrient deficiency.
Torabi
Sepideh
S
Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.
Wissuwa
Matthias
M
Heidari
Manzar
M
Naghavi
Mohammad-Reza
MR
Gilany
Kambiz
K
Hajirezaei
Mohammad-Reza
MR
Omidi
Mansoor
M
Yazdi-Samadi
Bahman
B
Ismail
Abdelbagi M
AM
Salekdeh
Ghasem Hosseini
GH
D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D002875:Chromosomes / Q000737:chemistry; D002952:Citric Acid Cycle; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D018384:Oxidative Stress; D010758:Phosphorus / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D012492:Salts / Q000737:chemistry; D015854:Up-Regulation
D000975:Antioxidants; D010940:Plant Proteins; D012333:RNA, Messenger; D012492:Salts; D010758:Phosphorus; D000040:Abscisic Acid; D011494:Protein Kinases; D002688:Chitinases