title: Long-term infusions of ghrelin and obestatin in early lactation dairy cows.
abstract: Ghrelin is an endogenous ligand of the growth hormone secretagogue receptor and a potential orexigenic agent in monogastrics and ruminants. Obestatin has been reported to have the opposite (anorexigenic) effect. Fifty one multiparous cows were randomly allocated to 1 of 3 groups (n = 17): a control group and 2 groups with cows continuously infused with 0.74 mumol/d of ghrelin (GHR group) or obestatin (OBE group) subcutaneously. Infusions began 21 d in milk, and treatments continued for 8 wk. Generalized linear models were used to determine the treatment effect on average daily and cumulative milk production and composition, and plasma ghrelin, growth hormone, insulin-like growth factor (IGF)-1, leptin, nonesterified fatty acids, and glucose. Mixed models, with cow included as a repeated effect, were used to determine if treatment effects differed by week postcalving for milk production, body weight, and body condition score (BCS; scale 1 to 10). Parity, breed, week of the year at calving, treatment, week postcalving, and the 2 wk preexperimental average of each measure (covariate) were included as fixed effects. Treatment did not affect dry matter intake. Cows infused with GHR lost more BCS (-0.71 units) over the 8-wk study period than the control (-0.23 BCS units) cows, and on average were thinner than cows in either of the other 2 treatments (0.2 BCS units). Consistent with the extra BCS loss in GHR cows, plasma IGF-1, glucose, and leptin concentrations were reduced and plasma nonesterified fatty acid concentrations were greater in GHR cows. Despite a numerical tendency for GHR cows to produce more milk (1,779 kg) than control (1,681 kg) or OBE (1,714 kg) cows during the 8-wk period, milk production differences were not statistically different. However, the timing of the numerical separation of the lactation curves coincided with the significant changes in BCS, IGF-1, and leptin. Results indicate a positive effect of ghrelin infusion on lipolysis. Further research is required to determine if the numerical increase in milk production, which coincides with the increased negative energy balance, is real.
authors.affiliation: DairyNZ, Hamilton, New Zealand. john.roche@DairyNZ.co.nz
authors.forename: J R
authors.initials: JR
authors.lastname: Roche
authors.forename: A J
authors.initials: AJ
authors.lastname: Sheahan
authors.forename: L M
authors.initials: LM
authors.lastname: Chagas
authors.forename: D
authors.initials: D
authors.lastname: Blache
authors.forename: D P
authors.initials: DP
authors.lastname: Berry
authors.forename: J K
authors.initials: JK
authors.lastname: Kay
mesh_terms: D000818:Animals; D001824:Body Constitution / Q000187:drug effects; D002417:Cattle / Q000502:physiology*; D003612:Dairying; D005260:Female; D054439:Ghrelin / Q000008:administration & dosage / Q000494:pharmacology*; D055104:Infusions, Subcutaneous; D007774:Lactation / Q000187:drug effects*; D016018:Least-Squares Analysis; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D011897:Random Allocation; D013997:Time Factors
chemical_list: D054439:Ghrelin