title: Trichinella pseudospiralis in pig from Croatia.
abstract: Trichinella spiralis and Trichinella britovi are species that are frequently found in domestic pigs and various sylvatic animals in Croatia. During routine trichinoscopy, non-encapsulated larvae were detected in the muscle tissue of a domestic pig. Artificial digestion revealed a larvae burden of 602 muscle larvae per gram of tissue. Tissue section analysis confirmed the presence of non-encapsulated larvae. Multiplex PCR identified the larvae as T. pseudospiralis. This observation is consistent with the reports of a local veterinary inspector who described the presence of non-encapsulated Trichinella in four individual cases over the last 2 years. This is the first report of T. pseudospiralis in Croatia and one of very few cases of T. pseudospiralis infection described in domestic pigs. The detection of non-encapsulated larvae stresses the need for implementation of artificial digestion instead of trichinoscopy for the detection and identification of Trichinella infections.
authors.affiliation: Faculty of Veterinary Medicine, Department for Parasitology and Parasitic Diseases, University of Zagreb, Heinzelova 55, Zagreb, Croatia. reljab@vef.hr
authors.forename: Relja
authors.initials: R
authors.lastname: Beck
authors.forename: Ana
authors.initials: A
authors.lastname: Beck
authors.forename: Snjezana
authors.initials: S
authors.lastname: Lucinger
authors.forename: Tihomir
authors.initials: T
authors.lastname: Florijancić
authors.forename: Ivica
authors.initials: I
authors.lastname: Bosković
authors.forename: Albert
authors.initials: A
authors.lastname: Marinculić
mesh_terms: D000818:Animals; D017523:Croatia / Q000453:epidemiology; D018482:Muscle, Skeletal / Q000469:parasitology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology*; D014234:Trichinella / Q000145:classification* / Q000166:cytology