ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells.
OBJECTIVE
Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro.


METHODS
PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor-beta (TGF-beta), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR.


RESULTS
ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF-beta and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15.


CONCLUSIONS
Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing.
Emecen
Pinar
P
Department of Periodontology, College of Dentistry, Ohio State University, Colombus, OH, USA.
Akman
Abdullah Cevdet
AC
Hakki
Sema Sezgin
SS
Hakki
Erdogan Esref
EE
Demiralp
Burak
B
Tözüm
Tolga Fikret
TF
Nohutcu
Rahime Meral
RM
D000818:Animals; D001857:Bone Matrix; D055396:Bone Morphogenetic Protein 2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018786:Bone Substitutes / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D006882:Hydroxyapatites / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D024042:Collagen Type I; D004338:Drug Combinations; D006882:Hydroxyapatites; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D042442:Vascular Endothelial Growth Factors; C407062:cell-binding peptide P-15; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I; D003094:Collagen