Mucoadjuvant properties of lipo- and glycoconjugated derivatives of oligochitosans.
Chitosan, (1-4)-2-amino-2-deoxy-beta-d-glucan, is a deacetylated form of chitin, an abundant biodegradable, positively charged natural polysaccharide. Chitosan is used for antigen delivery through mucosal barrier due to its ability to disrupt tight junctions. Here we produced new water-soluble low-molecular weight chitosan (LMW-Chi) lipid derivatives and compared their ability to stimulate humoral response with the effect of unmodified LMW-Chi or its oligosaccharide derivatives. LMW-Chi effectively penetrated into macrophage-like, lymphoid and epithelial cells. It also stimulated in mice IgG production to model proteins delivered either by subcutaneous or intranasal routes. Adjuvant effect of chitosan derivatives was comparable to or lower than that of unmodified LMW-Chi. Thus, it is possible that adjuvant effect is induced by unmodified glucosamine units of chitosan.
Svirshchevskaya
E V
EV
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, Moscow, Russia.
Alekseeva
L G
LG
Reshetov
P D
PD
Phomicheva
N N
NN
Parphenyuk
S A
SA
Ilyina
A V
AV
Zueva
V S
VS
Lopatin
S A
SA
Levov
A N
AN
Varlamov
V P
VP
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology; D048271:Chitosan; D006001:Glycoconjugates; D007074:Immunoglobulin G / Q000096:biosynthesis; D008055:Lipids; D051379:Mice; D008970:Molecular Weight; D009844:Oligosaccharides; D012995:Solubility; D013329:Structure-Activity Relationship
D000276:Adjuvants, Immunologic; D006001:Glycoconjugates; D007074:Immunoglobulin G; D008055:Lipids; D009844:Oligosaccharides; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan