title: High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.
abstract: The demand for high-throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for handling of nearly 300 different proteins per week. The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated.
authors.affiliation: School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Tegel
authors.forename: Johanna
authors.initials: J
authors.lastname: Steen
authors.forename: Anna
authors.initials: A
authors.lastname: Konrad
authors.forename: Hero
authors.initials: H
authors.lastname: Nikdin
authors.forename: Katarina
authors.initials: K
authors.lastname: Pettersson
authors.forename: Maria
authors.initials: M
authors.lastname: Stenvall
authors.forename: Samuel
authors.initials: S
authors.lastname: Tourle
authors.forename: Ulla
authors.initials: U
authors.lastname: Wrethagen
authors.forename: LanLan
authors.initials: L
authors.lastname: Xu
authors.forename: Louise
authors.initials: L
authors.lastname: Yderland
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.forename: Sophia
authors.initials: S
authors.lastname: Hober
authors.forename: Jenny
authors.initials: J
authors.lastname: Ottosson
mesh_terms: D001331:Automation; D001709:Biotechnology / Q000295:instrumentation*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D010455:Peptides; D011994:Recombinant Proteins