[Expression of hnRNP K in lung adenocarcinoma cells].
OBJECTIVE
To examine the effects of hnRNP K on the growth and apoptosis of lung adencarcinoma cells in vitro.


METHODS
The expressions of hnRNP K were detected by the SP method of immunohistochemistry in 70 lung adencarcinoma tissue samples. The hnRNP K siRNA was transfected into human lung adencarcinoma cell line A549 with Lipofectamine 2000. The cells transfected with siRNAn and untreated served as controls. The inhibitory effect of siRNA on the expression of hnRNP K mRNA and protein was detected by RT-PCR and Western blot. The change in cell cycling and cell apoptosis of siRNA-treated cells was detected by flow cytometry.


RESULTS
Positive expressions of hnRNP K were found in 38.5%, 95.2% and 91.7% of lung adencarcinoma tissues in tumors with a diameter of < or =3 cm, 3-5 cm and > or =5 cm respectively (P < 0.01). The expressions of hnRNP K mRNA were significantly inhibited in siRNA- transfected cells compared with those in controls (P < 0.05). hnRNP K protein decreased in hnRNP K siRNA-transfected cells. siRNA targeting human hnRNP K inhibited the growth of lung adencarcinoma cell line A549 and the distribution of the cell cycle, with an apoptosis rate of 4.79%. Twenty four hours after transfection, the number of cells in G0/G1 phase increased from 37.21% to 85.60%, while the number of cells in S and G2/M phases decreased from 47.71% and 13.00% to 13.50% and 0.32%, respectively.


CONCLUSIONS
The expression of hnRNP K is closely correlated with the size of lung adencarcinoma. hnRNP K siRNA inhibits the growth aNd increases the apoptosis of lung adencarcinoma cells.
Tang
Feng-ming
FM
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Wei-min
WM
Chen
Yan
Y
Wang
Dong-mei
DM
Han
Juan
J
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017209:Apoptosis / Q000235:genetics*; D049109:Cell Proliferation; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D012333:RNA, Messenger; D034741:RNA, Small Interfering