title: Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells.
abstract: Dynamin 2 has been reported to be implicated in phagocytosis. However, the mode of action of dynamin is poorly understood. In this study, we examined whether dynamin 2 participates in actin assembly during phagocytosis in Sertoli cells. In the presence of dynasore, a dynamin inhibitor, phagocytosis was reduced by 60-70% in Sertoli cells and macrophages. Scanning electron microscopy revealed that Sertoli cells treated with dynasore were unable to form phagocytic cups. In addition, dysfunction of dynamin 2 reduced both actin polymerization and recruitment of actin and dynamin 2 to phosphatidylinositol (4,5) bisphosphate [PI(4,5)P(2)]-containing liposomes. The formation of dynamin 2-positive ruffles of Sertoli cells was decreased by 60-70% by sequestering PI(4,5)P(2) either by expression of PH domain of PLCdelta or treatment with neomycin. These results strongly suggest that dynamin 2 is involved in actin dynamics and the formation of dynamin 2-positive ruffles during phagocytosis.
authors.affiliation: Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Otsuka
authors.forename: Tadashi
authors.initials: T
authors.lastname: Abe
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yagisawa
authors.forename: Kohji
authors.initials: K
authors.lastname: Takei
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D034285:Dynamin II / Q000037:antagonists & inhibitors / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000494:pharmacology; D051381:Rats; D012708:Sertoli Cells / Q000201:enzymology / Q000276:immunology*
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D019269:Phosphatidylinositol 4,5-Diphosphate; D034285:Dynamin II