Chaperone-like activity of heme group against amyloid-like fibril formation by hen egg ovalbumin: possible mechanism of action.
It is well accepted that porphyrins related to heme, are effectively able to act as pharmaceutical. For instance, they were found to interfere with the aggregation of beta-amyloid peptide. In present study, the influence of heme concentration on the amyloid-type aggregation of ovalbumin was investigated. We provided experimental evidence of heme's prevention of ovalbumin aggregation in the heat-denaturation process using spectroscopic measurements. Different types of interactions were suggested to be involved in heme-ovalbumin amyloid communication. Since the consequence of heme-attenuated fibrillogenesis is yet to be identified, additional data on chaperoning role of heme may help us to manage amyloid aggregation processes.
Khodarahmi
Reza
R
Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, P.O. Box 67145-1673, Kermanshah University of Medical Sciences, Kermanshah, Iran. rkhodarahmi@mbrc.ac.ir
Soori
Hosnieh
H
Karimi
Seyyed Arash
SA
D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D003224:Congo Red; D004121:Dimethyl Sulfoxide; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005453:Fluorescence; D006418:Heme / Q000378:metabolism*; D006358:Hot Temperature; D018832:Molecular Chaperones / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism*; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D014270:Trifluoroethanol
D000682:Amyloid; D018832:Molecular Chaperones; D003224:Congo Red; D006418:Heme; D014270:Trifluoroethanol; D010047:Ovalbumin; D004121:Dimethyl Sulfoxide