title: Generation and characterization of novel canine malignant mast cell line CL1.
abstract: Studies using the currently available malignant canine mast cell lines and bone marrow-derived cultured mast cells (BMCMCs) have provided an in-depth understanding of normal and neoplastic canine mast cell biology. However, many of the currently available malignant canine mast cell lines possess limitations, including loss of cell surface markers and inability to bind canine IgE. We have recently generated a novel mast cell line, CL1, from an 11-year-old spayed female Labrador retriever diagnosed with systemic mastocytosis and neoplastic effusion. The CL1 cells express KIT, FcepsilonRI, CD44, CD45, CD14, CD11a, CD11b and CD18 as well as chymase. Interestingly, these cells express wild-type KIT, with no evidence of autophosphorylation, but are able to proliferate independently without the addition of exogenous stem cell factor (SCF), KIT ligand. However, stimulation of CL1 cells with SCF induces KIT phosphorylation promoting cell proliferation. The CL1 cells retain functional properties of mast cells, degranulating in a dose-dependent manner in response to both IgE cross-linking and chemical stimulation. Lastly, cytogenetic evaluation revealed several recurrent tumor-associated chromosome copy number imbalances in the CL1 line. In summary, the CL1 cell line possesses phenotypic and functional properties similar to those found in canine BMCMCs, and will likely be a useful tool to study mast cell biology, factors regulating transformation of mast cells, cytogenetic abnormalities in mast cell tumors, and novel preclinical therapies.
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Tzu-Yin
authors.initials: TY
authors.lastname: Lin
authors.forename: Rachael
authors.initials: R
authors.lastname: Thomas
authors.forename: Pei-Chien
authors.initials: PC
authors.lastname: Tsai
authors.forename: Matthew
authors.initials: M
authors.lastname: Breen
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: London
mesh_terms: D000818:Animals; D001483:Base Sequence; D015550:Cell Degranulation; D045744:Cell Line, Tumor; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D004285:Dogs; D005260:Female; D055106:Genome-Wide Association Study; D016130:Immunophenotyping; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology* / Q000502:physiology; D034721:Mastocytosis, Systemic / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm; D019009:Proto-Oncogene Proteins c-kit