High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.
The demand for high-throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for handling of nearly 300 different proteins per week. The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated.
Tegel
Hanna
H
School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
Steen
Johanna
J
Konrad
Anna
A
Nikdin
Hero
H
Pettersson
Katarina
K
Stenvall
Maria
M
Tourle
Samuel
S
Wrethagen
Ulla
U
Xu
LanLan
L
Yderland
Louise
L
Uhlén
Mathias
M
Hober
Sophia
S
Ottosson
Jenny
J
D001331:Automation; D001709:Biotechnology / Q000295:instrumentation*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013053:Spectrophotometry
D010455:Peptides; D011994:Recombinant Proteins