Inhibition of cyclooxygenase activity blocks cell-to-cell spread of human cytomegalovirus.
Human cytomegalovirus has previously been shown to induce the accumulation of cyclooxygenase-2 RNA, protein, and enzyme activity. High doses of cyclooxygenase inhibitors substantially block viral replication in cultured fibroblasts. However, doses corresponding to the level of drug achieved in the plasma of patients have little effect on the replication of human cytomegalovirus in cultured cells. Here, we demonstrate that two nonsteroidal anti-inflammatory drugs, tolfenamic acid and indomethacin, markedly reduce direct cell-to-cell spread of human cytomegalovirus in cultured fibroblasts. The block is reversed by addition of prostaglandin E2, proving that it results from the action of the drugs on cyclooxygenase activity. Because direct cell-to-cell spread likely contributes importantly to pathogenesis of the virus, we suggest that nonsteroidal anti-inflammatory drugs might help to control human cytomegalovirus infections in conjunction with other anti-viral treatments.
Schröer
Jörg
J
Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Shenk
Thomas
T
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000188:drug therapy* / Q000378:metabolism; D015232:Dinoprostone / Q000494:pharmacology; D004347:Drug Interactions; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000998:Antiviral Agents; D016861:Cyclooxygenase Inhibitors; D014764:Viral Proteins; D062367:ortho-Aminobenzoates; C009500:tolfenamic acid; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D007213:Indomethacin