[Determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food by high performance anion exchange chromatography].
OBJECTIVE
To develop a method for analysis of eight kinds of monosaccharides using high performance anion exchange chromatography.


METHODS
Amino PAC PA-10 (2 x 250 mm) column with a pulse amperometric detector with an Au working electrode and an Ag/AgCl reference electrode was applied to determine the monosaccharides.


RESULTS
The linear range was 0.1-5.0 microg/mL and the detection limits were 2-26 microg/L. The relative standard derivation for retain time and area of standard solution were 0.34%-7.42% and 0.02%-2.16% respectively. The satisfactory results were obtained for analysis of honey and polysaccharide hydrolyzate of functional food with the proposed method. The average recoveries were 81.4%-122.0%. The method was sensitive and with high precision.


CONCLUSIONS
This method was available for determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food.
Zou
Xiao-li
XL
Department of Sanitary Technology, West China School of Pulblic Health, Sichuan University, Chengdu 610041, China.
Jiang
Shui
S
Zheng
Bo
B
Zeng
Hong-yan
HY
Yan
Ci-ling
CL
Xie
Qi
Q
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006722:Honey / Q000032:analysis*; D006868:Hydrolysis; D009005:Monosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry*; D012680:Sensitivity and Specificity
D009005:Monosaccharides; D011134:Polysaccharides