Iron-deficiency anaemia enhances red blood cell oxidative stress.
Oxidative stress associated with iron deficiency anaemia in a murine model was studied feeding an iron-deficient diet. Anaemia was monitored by a decrease in hematocrit and haemoglobin. For the 9 week study an increase in total iron binding capacity was also demonstrated. Anaemia resulted in an increase in red blood cells (RBC) oxidative stress as indicated by increased levels of fluorescent heme degradation products (1.24-fold after 5 weeks; 2.1-fold after 9 weeks). The increase in oxidative stress was further confirmed by elevated levels of methemoglobin for mice fed an iron-deficient diet. Increased haemoglobin autoxidation and subsequent generation of ROS can account for the shorter RBC lifespan and other pathological changes associated with iron-deficiency anaemia.
Nagababu
Enika
E
Molecular Dynamics Section, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
Gulyani
Seema
S
Earley
Christopher J
CJ
Cutler
Roy G
RG
Mattson
Mark P
MP
Rifkind
Joseph M
JM
D018798:Anemia, Iron-Deficiency / Q000473:pathology*; D000818:Animals; D001835:Body Weight; D004912:Erythrocytes / Q000378:metabolism*; D006400:Hematocrit; D006418:Heme / Q000737:chemistry; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008297:Male; D008706:Methemoglobin / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry
D006454:Hemoglobins; D006418:Heme; D008706:Methemoglobin; D007501:Iron; D010100:Oxygen