title: The adverse effect of dichlorodiphenyltrichloroethane (DDT) and its metabolite (DDE) on the secretion of prostaglandins and oxytocin in bovine cultured ovarian and endometrial cells.
abstract: The influence of chlorinated hydrocarbon insecticide-dichlorodiphenyltrichloroethane (DDT) and its metabolite (DDE) on the uterine prostaglandin (PGF2alpha and PGE2) and ovarian oxytocin (OT) secretion was investigated. Endometrial, granulosa and luteal cells from cows and heifers on 8-12 days of estrous cycle were treated (24-72h) with DDT, mixture or individual isomer (p,p'-, o,p'-) of DDE (0.1, 1 or 10ng/ml). The viability of cells and the concentrations of hormones in medium were determinted. Neither DDT nor DDE affected (P>0.05) the viability of cells, while they stimulated (P<0.05) PGF2alpha and reduced (P<0.001) PGE2 secretion from endometrial cells. Hence the ratio of PGF2alpha to PGE2 was markedly changed. Used pollutants also increased (P<0.05) OT secretion from ovarian cells. It is concluded that DDT and its metabolite impair PGF2alpha, PGE2 and OT secretion from uterus and ovary, respectively, and this way they can affect the course of estrous cycle in cattle.
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Street 10, 10-747 Olsztyn, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Wrobel
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Mlynarczuk
authors.forename: Jan
authors.initials: J
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003634:DDT / Q000633:toxicity*; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D010121:Oxytocin / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism*
chemical_list: D007306:Insecticides; D011453:Prostaglandins; D003633:Dichlorodiphenyl Dichloroethylene; D010121:Oxytocin; D015237:Dinoprost; D003634:DDT; D015232:Dinoprostone