title: Safety assessment of bacterial choline oxidase protein introduced in transgenic crops for tolerance against abiotic stress.
abstract: Genetically modified crops have resistance to abiotic stress by introduction of choline oxidase protein. In the present study, the safety of choline oxidase protein derived from Arthrobacter globiformis was assessed for toxicity and allergenicity. The protein was stable at 90 degrees C for 1 h. Toxicity studies of choline oxidase in mice showed no significant difference (p > 0.05) from control in terms of growth, body weight, food consumption, and blood biochemical indices. Histology of gut tissue of mice fed protein showed normal gastric mucosal lining and villi in jejunum and ileum sections. Specific IgE in serum and IL-4 release in splenic culture supernatant were low in choline oxidase treated mice, comparable to control. Intravenous challenge with choline oxidase did not induce any adverse reaction, unlike ovalbumin group mice. Histology of lung tissues from choline oxidase sensitized mice showed normal airways, whereas ovalbumin-sensitized mice showed inflamed airways with eosinophilic infiltration and bronchoconstriction. ELISA carried out with food allergic patients' sera revealed no significant IgE affinity with choline oxidase. Also, choline oxidase did not show any symptoms of toxicity and allergenicity in mice.
authors.affiliation: Institute of Genomics and Integrative Biology (CSIR), Delhi, India.
authors.forename: Abinav K
authors.initials: AK
authors.lastname: Singh
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Singh
authors.forename: G B K S
authors.initials: GB
authors.lastname: Prasad
authors.forename: Shailendra N
authors.initials: SN
authors.lastname: Gaur
authors.forename: Naveen
authors.initials: N
authors.lastname: Arora
mesh_terms: D000328:Adult; D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000633:toxicity*; D000818:Animals; D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000633:toxicity*; D002478:Cells, Cultured; D004795:Enzyme Stability; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D030841:Food, Genetically Modified / Q000592:standards; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D030821:Plants, Genetically Modified / Q000276:immunology* / Q000502:physiology / Q000633:toxicity*; D011897:Random Allocation; D013154:Spleen / Q000276:immunology; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D000429:Alcohol Oxidoreductases; C019888:choline oxidase