[Monocytes are more susceptible in vitro in rhesus macaques of Chinese origin to recombinant serotype 5 adenovirus with higher preexisting vector specific neutralizing antibody titer].
Human adenovirus serotype 5 (HAd5) infect dendritic cells with low efficiency which restricts the use of HAd5 as an antigen carrying vector in such cells. Aiming to find a novel strategy to detour the traditional method for more convenient clinical use, peripheral blood mononuclear cells isolated from Chinese rhesus macaques were chosen as the target cells for HAd5. In vitro infection protocol was optimized which indicated centrifugation at 1000g could ease the entry of adenovirus. By this protocol, CD14 positive monocytes were infected at high efficiencies (> 80%), and about 10% of natural killer cells were infected; while T and B lymphocytes were rarely infected. Interestingly and importantly, it was the first time to report that in our in vitro study monocytes were more susceptible to HAd5-EGFP in macaques with higher preexisting vector specific neutralizing antibody titers. This phenomenon indicates an expansion of application of adenovirus based vectors for vaccine development and clinical use, especially for the population with preexisting neutralizing antibodies.
Zhuang
Qiu-Chuan
QC
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. zhuang_qiuchuan@gibh.ac.cn
Sun
Cai-Jun
CJ
Li
Feng
F
Feng
Li-Qiang
LQ
Liu
Yi-Chu
YC
Chen
Ling
L
D000256:Adenoviridae / Q000145:classification / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008253:Macaca mulatta; D008297:Male; D009000:Monocytes / Q000821:virology*; D009500:Neutralization Tests; D011995:Recombination, Genetic; D012703:Serotyping
D000914:Antibodies, Viral; D018950:Lipopolysaccharide Receptors