Effect of cigarette filters on the chemical composition and in vitro biological activity of cigarette mainstream smoke.
The objective of this study was to evaluate the effects of cigarette filters on the chemical composition and toxicity of cigarette mainstream smoke. In this work, we used three types of cigarettes, including non-filter 2R4F cigarettes, cellulose acetate (CA)-filter 2R4F cigarettes, and carbon dual-filter 2R4F cigarettes. The cytotoxicity of TPM obtained from the filter cigarettes was not different from that of the non-filter cigarettes on an equal TPM basis. However, the EC50 value of GVP from carbon-filter cigarettes were 40.9 puffs/L, thereby indicating the cytotoxicity of these cigarettes was approximately 37% and 21% lower than non-filter and CA-filter cigarettes, respectively. The cytotoxicity of GVP was correlated with carbonyl components. The mutagenicity of TPM obtained from non-filter cigarettes, calculated on an equal TPM basis, was up to 30-40% lower than that of the filter cigarettes. When calculated on a per cigarette basis, the mutagenicity of CA or carbon-filter cigarettes was found to be 35% lower than that of the non-filter cigarettes. The results of chemical composition analyses revealed that the observed increase in aromatic amine compound yields on an equal TPM basis in filter cigarettes may be related with the mutagenic activity determined in Ames assays.
Shin
Han-Jae
HJ
KT&G Central Research Institute, Laboratory of Biochemistry, 302 Shinseong-Dong, Yuseong-Gu, Daejeon 305-805, Republic of Korea. hjshin@ktng.com
Sohn
Hyung-Ok
HO
Han
Jung-Ho
JH
Park
Chul-Hoon
CH
Lee
Hyeong-Seok
HS
Lee
Dong-Wook
DW
Hwang
Keon-Joong
KJ
Hyun
Hak-Chul
HC
D000818:Animals; D016466:CHO Cells; D002244:Carbon / Q000737:chemistry; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins; D004305:Dose-Response Relationship, Drug; D005374:Filtration / Q000295:instrumentation*; D009152:Mutagenicity Tests; D009153:Mutagens; D012486:Salmonella typhimurium / Q000187:drug effects; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry / Q000633:toxicity*
D003603:Cytotoxins; D009153:Mutagens; D012906:Smoke; D002244:Carbon