Enzyme catalyzed site-specific protein labeling and cell imaging with quantum dots.
We have developed an efficient method for one-step covalent labeling of cell surface proteins with quantum dots based on enzyme catalyzed site-specific modification of short peptide tags.
Sunbul
Murat
M
Department of Chemistry, The University of Chicago, 929 East 57th Street, GCIS E505A, Chicago, IL 60637, USA.
Yen
Michelle
M
Zou
Yekui
Y
Yin
Jun
J
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D003065:Coenzyme A / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry; D045663:Quantum Dots; D014162:Transfection
D005456:Fluorescent Dyes; D008565:Membrane Proteins; D010455:Peptides; D003065:Coenzyme A