Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis.
Glucosyl-3-phosphoglycerate synthase (GpgS) is a key enzyme that catalyses the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in mycobacteria. These important molecules are believed to be involved in the regulation of fatty-acid and mycolic acid synthesis. The enzyme belongs to the recently defined GT81 family of retaining glycosyltransferases (CAZy, Carbohydrate-Active Enzymes Database; see http://www.cazy.org). Here, the purification, crystallization and preliminary crystallographic analysis are reported of GpgS from Mycobacterium tuberculosis and of its complex with UDP. GpgS crystals belonged to space group I4, with unit-cell parameters a = 98.85, b = 98.85, c = 127.64 A, and diffracted to 2.6 A resolution. GpgS-UDP complex crystals belonged to space group I4, with unit-cell parameters a = 98.32, b = 98.32, c = 127.96 A, and diffracted to 3.0 A resolution.
Gest
Petra
P
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-168, USA.
Kaur
Devinder
D
Pham
Ha T
HT
van der Woerd
Mark
M
Hansen
Emily
E
Brennan
Patrick J
PJ
Jackson
Mary
M
Guerin
Marcelo E
ME
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000378:metabolism; D014530:Uridine Diphosphate / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D014530:Uridine Diphosphate; D016695:Glycosyltransferases