Rhusonoside A, a new megastigmane glycoside from Rhus sylvestris, increases the function of osteoblastic MC3T3-E1 cells.
Chemical investigation of the stems and leaves of Rhus Sylvestris afforded a new megastigmane glycoside named rhusonoside A ( 1), along with four other known compounds: dihydroquercetin ( 2), astragalin ( 3), hyperin ( 4), and kaempferol-3- O-rutinoside ( 5). Their structures were determined by a combination of spectroscopic analysis and application of the modified Mosher's method. The effect of compounds 1 - 5 on the function of osteoblastic MC3T3-E1 cells was examined by determining cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Rhusonoside A ( 1) significantly increased the function of osteoblastic MC3T3-E1 cells. Cell viability, ALP activity, and collagen synthesis were increased dose dependently, up to 155.39 %, 171.27 %, and 134.25 %, respectively, of the basal value at 10 muM ( P < 0.05). In addition, 0.1 muM of compound 1 significantly increased mineralization of MC3T3-E1 cells to 142.78 % ( P < 0.05) of the basal value.
Ding
Yan
Y
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Nguyen
Huu Tung
HT
Choi
Eun Mi
EM
Bae
Kihwan
K
Kim
Young Ho
YH
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D003511:Cyclohexanols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D045792:Norisoprenoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D018547:Plant Stems; D029045:Rhus / Q000737:chemistry*
D003511:Cyclohexanols; D005960:Glucosides; D006027:Glycosides; D045792:Norisoprenoids; D010936:Plant Extracts; C539439:rhusonoside A; D003094:Collagen; D000469:Alkaline Phosphatase