title: Insights into outer membrane protein crystallization.
abstract: Outer membrane proteins are structurally distinct from those that reside in the inner membrane and play important roles in bacterial pathogenicity and human metabolism. X-ray crystallography studies on >40 different outer membrane proteins have revealed that the transmembrane portion of these proteins can be constructed from either beta-sheets or less commonly from alpha-helices. The most common architecture is the beta-barrel, which can be formed from either a single anti-parallel sheet, fused at both ends to form a barrel or from multiple peptide chains. Outer membrane proteins exhibit considerable rigidity and stability, making their study through x-ray crystallography particularly tractable. As the number of structures of outer membrane proteins increases a more rational approach to their crystallization can be made. Herein we analyse the crystallization data from 53 outer membrane proteins and compare the results to those obtained for inner membrane proteins. A targeted sparse matrix screen for outer membrane protein crystallization is presented based on the present analysis.
authors.affiliation: Division of Molecular Biosciences, Membrane Protein Crystallography Group, Imperial College London, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Newstead
authors.forename: Jeanette
authors.initials: J
authors.lastname: Hobbs
authors.forename: Davina
authors.initials: D
authors.lastname: Jordan
authors.forename: Elisabeth P
authors.initials: EP
authors.lastname: Carpenter
authors.forename: So
authors.initials: S
authors.lastname: Iwata
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002021:Buffers; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D003902:Detergents; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012492:Salts
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002021:Buffers; D003902:Detergents; D012492:Salts; D011092:Polyethylene Glycols