title: Infusion of cytomegalovirus specific cytotoxic T lymphocytes from a sero-negative donor can facilitate resolution of infection and immune reconstitution.
abstract: We report a stem cell transplant patient with a therapy-refractory cytomegalovirus (CMV) infection who received CMV-specific T cells from his sero-negative stem cell donor. This donor received the Towne strain CMV vaccine, and T cells were expanded using monocytes pulsed with pp65 overlapping peptides. CMV DNA decreased after the CTL infusion, and CMV-specific cytotoxicity increased. This strategy could be implemented in similar situations or with persistent viremia post-transplant.
authors.affiliation: Department of Pediatrics, Blood and Marrow Transplant Program, University of California, San Francisco, CA.
authors.forename: Biljana
authors.initials: B
authors.lastname: Horn
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Kimberly
authors.initials: K
authors.lastname: Dunham
authors.forename: Mindy
authors.initials: M
authors.lastname: Stamer
authors.forename: Stuart
authors.initials: S
authors.lastname: Adler
authors.forename: Morton
authors.initials: M
authors.lastname: Cowan
authors.forename: Kenneth
authors.initials: K
authors.lastname: Lucas
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000628:therapy*; D022482:Cytomegalovirus Vaccines; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008297:Male; D010750:Phosphoproteins / Q000276:immunology*; D033581:Stem Cell Transplantation / Q000009:adverse effects; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology*; D055815:Young Adult
chemical_list: D022482:Cytomegalovirus Vaccines; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa