title: Microarray and biochemical analysis of bufalin-induced apoptosis of HL-60 Cells.
abstract: Bufalin is a natural toxin with anti-leukemic properties. It induces cell differentiation and apoptosis, as well as increasing the sensitivity of leukemia cells to other chemotherapeutic agents. We investigated the biological effects and molecular mechanisms of bufalin triggered apoptosis in HL-60 cells by gene expression profiling. The broad transcriptional response to bufalin was consistent with bufalin's action of regulating HL-60 cell proliferation and apoptosis, as well as its synergistic effect with other drugs. Further transcription factor ELISA experiments suggested that the transcription factors NFkappaB and AP-1 were activated to promote bufalin-induced HL-60 cell apoptosis. Our study provides new insights into the molecular mechanisms of bufalin, might prove to be beneficial in leukemia therapy.
authors.affiliation: Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing 100084, People's Republic of China.
authors.forename: Ailiang
authors.initials: A
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Guo
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Keith
authors.initials: K
authors.lastname: Mitchelson
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
mesh_terms: D017209:Apoptosis; D002018:Bufanolides / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D016328:NF-kappa B / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D018808:Transcription Factor AP-1 / Q000096:biosynthesis
chemical_list: D002018:Bufanolides; D016328:NF-kappa B; D018808:Transcription Factor AP-1; C022777:bufalin