Profiling of root canal bacterial communities associated with chronic apical periodontitis from Brazilian and Norwegian subjects.
The aim of this study was to compare the bacterial community profiles of the root canal microbiota associated with chronic apical periodontitis from Brazilian and Norwegian patients using the denaturing gradient gel electrophoresis (DGGE) and the ribosomal intergenic spacer analysis (RISA) approaches. DNA extracted from root canal samples was subjected to polymerase chain reaction using primers appropriate for further DGGE or RISA analysis. The resulting banding patterns representative of the bacterial community structures in samples from the two locations were compared. DGGE and RISA fingerprints showed a great interindividual variability in the bacterial community profiles, irrespective of the geographic location of the patient. However, similarities among the bacterial community DGGE profiles revealed the existence of a geography-related pattern.
Siqueira
José F
JF
Department of Endodontics and Molecular Microbiology, Estácio de Sá University, Rio de Janeiro, Brazil. jf_siqueira@yahoo.com
Rôças
Isabela N
IN
Debelian
Gilberto J
GJ
Carmo
Flávia L
FL
Paiva
Simone S M
SS
Alves
Flávio R F
FR
Rosado
Alexandre S
AS
D000328:Adult; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001938:Brazil; D002908:Chronic Disease; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D009664:Norway; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012338:RNA, Ribosomal, 23S / Q000032:analysis
D017931:DNA Primers; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S