title: S100beta and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury.
abstract: OBJECTIVE
  The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100beta are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury.
  
  
  METHODS
  Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve.
  
  
  RESULTS
  FGF-2 and S100beta are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100beta positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100beta positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2.
  
  
  CONCLUSIONS
  Reactive peripheral glial cells synthesizing FGF-2 and S100beta may be important in wound repair and restorative events in the lesioned peripheral nerves.
authors.affiliation: Department of Neurology, School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Duobles
authors.forename: Thais de Sousa
authors.initials: Tde S
authors.lastname: Lima
authors.forename: Beatriz de Freitas Azevedo
authors.initials: Bde F
authors.lastname: Levy
authors.forename: Gerson
authors.initials: G
authors.lastname: Chadi
mesh_terms: D000818:Animals; D019771:Axotomy; D015153:Blotting, Western; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009409:Nerve Crush; D009414:Nerve Growth Factors / Q000032:analysis / Q000378:metabolism*; D019899:Paracrine Communication; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000502:physiology / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism*; D027161:Satellite Cells, Perineuronal / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000293:injuries / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D016222:Fibroblast Growth Factor 2