title: An n-3 PUFA-rich microalgal oil diet protects to a similar extent as a fish oil-rich diet against AOM-induced colonic aberrant crypt foci in F344 rats.
abstract: The chemopreventive effects of high fat microalgal oil diet on azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were studied in male Fischer 344 rats following 8 weeks of dietary treatment. These effects were compared to the effects of high fat fish oil and high fat corn oil diets to determine whether microalgal oil is a good alternative for fish oil regarding protection against colorectal cancer. Despite the difference in fatty acid composition and total amount of n-3 polyunsaturated fatty acids (PUFAs) between microalgal oil and fish oil, both these oils gave the same 50% reduction of AOM-induced ACF when compared to corn oil. To determine whether oxidative stress could play a role in the chemoprevention of colorectal cancer by n-3 PUFAs, feces and caecal content were examined using the TBA assay. The results showed that lipid peroxidation does occur in the gastrointestinal tract. As several lipid peroxidation products of n-3 PUFAs can induce phase II detoxifying enzymes by an EpRE-mediated pathway, the in vivo results suggest that this route may contribute to n-3 PUFA-mediated chemoprevention. All in all, n-3 PUFA-rich oil from microalgae is as good as fish oil regarding chemoprevention in the colon of the rat.
authors.affiliation: Division of Toxicology, Wageningen University and Research Centre, PO Box 8000, 6700 EA Wageningen, The Netherlands.
authors.forename: Vincent A
authors.initials: VA
authors.lastname: van Beelen
authors.forename: Bert
authors.initials: B
authors.lastname: Spenkelink
authors.forename: Hans
authors.initials: H
authors.lastname: Mooibroek
authors.forename: Lolke
authors.initials: L
authors.lastname: Sijtsma
authors.forename: Dirk
authors.initials: D
authors.lastname: Bosch
authors.forename: Ivonne M C M
authors.initials: IM
authors.lastname: Rietjens
authors.forename: Gerrit M
authors.initials: GM
authors.lastname: Alink
mesh_terms: D000821:Animal Feed; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D003314:Corn Oil / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage*; D004195:Disease Models, Animal; D056890:Eukaryota / Q000737:chemistry*; D015525:Fatty Acids, Omega-3; D005395:Fish Oils / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010938:Plant Oils; D003314:Corn Oil; D001397:Azoxymethane