[Effects of three immunosuppressive agents and simvastatin on cell-proliferation and endothelin secretion of human endothelial cells].
OBJECTIVE
To evaluate the effects of four agents including cyclophosphamide, thalidomide, total glucosides of peony (TGP) and simvastatin on cell-proliferation and endothelin-1 secretion of human endothelial cells (ECs).


METHODS
EA. hy926 cells were cultured until confluence was achieved, then incubated separately for 24 h, 48 h and 72 h with various concentration of these four agents. The proliferation of ECs was detected with 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT). The amount of endothelin-1 in supernatants of ECs was determined with enzyme linked immunosorbent assay.


RESULTS
(1) Cyclophosphamide, TGP and simvastatin inhibited ECs proliferation in a dose dependent manner at 72 h (P <0.05). (2) Cyclophosphamide (50-2000 micromol/L) and simvastatin (5-10 micromol/L) decreased endothelin-1 secretion but not in dose dependent manner.


CONCLUSIONS
Cyclophosphamide, TGP and simvastatin can inhibit ECs proliferation. Cyclophosphamide and simvastatin can decrease endothelin-1 secretion of human ECs.
Zhang
Shang-Zhu
SZ
Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
Li
Meng-Tao
MT
He
Jian-Guo
JG
Zeng
Xiao-Feng
XF
D002455:Cell Division; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D019821:Simvastatin / Q000494:pharmacology*
D019332:Endothelin-1; D007166:Immunosuppressive Agents; D019821:Simvastatin