Endoxanthanase, a novel beta-D-glucanase hydrolyzing backbone linkage of intact xanthan from newly isolated Microbacterium sp. XT11.
A novel endoxanthanase was produced and isolated from the culture of Microbacterium sp. XT11 growing on xanthan. Optimum pH and temperature for the enzyme activity were 6.0 and 35-40 degrees C, respectively. The endoxanthanase cleaves the backbone endo-beta-1,4-glucosidic linkage of the xanthan molecule, which is specific to the intact xanthan. However, the lyase-treated xanthan or carboxymethyl cellulose could not be cleaved by endoxanthanase.
Li
Bing
B
Department of Bio & Food Engineering, Dalian College of Light Industry, Dalian, People's Republic of China.
Guo
Jiqiang
J
Chen
Wenfu
W
Chen
Xiaoyi
X
Chen
Ligang
L
Liu
Zhiwen
Z
Li
Xianzhen
X
D001665:Binding Sites; D044644:Endo-1,3(4)-beta-Glucanase / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D006094:Gram-Positive Bacteria / Q000201:enzymology*; D006868:Hydrolysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011485:Protein Binding
D011135:Polysaccharides, Bacterial; D044644:Endo-1,3(4)-beta-Glucanase; C002563:xanthan gum