title: Immobilization of catalysts of biological interest on porous oxidized silicon surfaces.
abstract: The present paper deals with the immobilization of redox mediators and proteins onto protected porous silicon surfaces to obtain their direct electrochemical reactions and to retain their bioactivities. This paper shows that MP-11 and viologens are able to establish chemical bonds with 3-aminopropyltriethoxylsilane-modified porous silicon surface. The functionalization of the surfaces have been fully characterized by energy dispersive X-ray analysis (EDX) and X-ray photoelectron spectroscopy (XPS) to examine the immobilization of these mediators onto the solid surface. Amperometric and open circuit potential measurements have shown the direct electron transfer between glucose oxidase and the electrode in the presence of the viologen mediator covalently linked to the 3-aminopropyltriethoxylsilane (APTES)-modified porous silicon surfaces.
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP, Brazil.
authors.forename: Wendel A
authors.initials: WA
authors.lastname: Alves
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Fiorito
authors.forename: Gerard
authors.initials: G
authors.lastname: Froyer
authors.forename: Fady
authors.initials: F
authors.lastname: El Haber
authors.forename: Luc
authors.initials: L
authors.lastname: Vellutini
authors.forename: Roberto M
authors.initials: RM
authors.lastname: Torresi
authors.forename: Susana I Córdoba
authors.initials: SI
authors.lastname: de Torresi
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism; D004583:Electrons; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013997:Time Factors
chemical_list: D011437:Propylamines; D012821:Silanes; D005949:Glucose Oxidase; D005947:Glucose; C477625:amino-propyl-triethoxysilane; D010100:Oxygen; D012825:Silicon