Integration of laser trapping for continuous and selective monitoring of photothermal response of a single microparticle.
Photothermal response (PTR) is an established pump and probe technique for real-time sensing of biological assays. Continuous and selective PTR monitoring is difficult owing to the Brownian motion changing the relative position of the target with respect to the beams. Integration of laser trapping with PTR is proposed as a solution. The proposed method is verified on red polystyrene microparticles. PTR is continuously monitored for 30 min. Results show that the mean relaxation time variation of the acquired signals is less than 5%. The proposed method is then applied to human red blood cells for continuous and selective PTR.
Vasudevan
Srivathsan
S
1Photonics Research Center, School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
Chen
George C K
GC
Ahluwalia
Balpreet Singh
BS
D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000502:physiology* / Q000528:radiation effects*; D006358:Hot Temperature; D052898:Optical Tweezers; D018511:Systems Integration; D013696:Temperature