(-)-Epigallocatechin gallate/gelatin layer-by-layer assembled films and microcapsules.
A new type of protein/polyphenol microcapsules on the basis of naturally occurring polyphenol (-)-epigallocatechin gallate (EGCG) and gelatin, type A, was obtained using the layer-by-layer (LbL) assembly method. The microcapsules show a more pronounced dependence of permeability on molecular weight of permeating substances than commonly used polyallylamine/polystyrene sulfonate capsules. The regularities of EGCG adsorption in alternation with type A and B gelatins have been investigated using quartz crystal microbalance and electrophoretic mobility measurements on microparticles and found to be dependent on gelatin properties. EGCG in the LbL assemblies retains its antioxidant activity. The kinetics of the reaction of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) cation-radicals with films consisting of 1-10 gelatin/EGCG bilayers is affected by film structure. The EGCG content in the protein/polyphenol film material is as high as 30% w/w. Encapsulation of EGCG via its alternated adsorption with gelatins can be a perspective way to new formulations containing the polyphenol for drug delivery applications.
Shutava
Tatsiana G
TG
Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, USA. shutova@ichnm.basnet.by
Balkundi
Shantanu S
SS
Lvov
Yuri M
YM
D000327:Adsorption; D000975:Antioxidants; D002214:Capsules / Q000737:chemistry*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D016503:Drug Delivery Systems; D004563:Electrochemistry; D004586:Electrophoresis; D005780:Gelatin / Q000737:chemistry*; D008567:Membranes, Artificial; D010539:Permeability
D000975:Antioxidants; D002214:Capsules; D008567:Membranes, Artificial; D002392:Catechin; D005780:Gelatin; C045651:epigallocatechin gallate