RGD peptide-immobilized electrospun matrix of polyurethane for enhanced endothelial cell affinity.
An Arg-Gly-Asp (RGD) peptide-immobilized electrospun matrix of polyurethane (PU) was developed for the enhanced affinity of endothelial cells (EC). The novel PU matrix was fabricated as a vascular shape using the electrospinning technique. Then, poly(ethylene glycol) (PEG) was immobilized on the porous PU matrix as a spacer, followed by conjugating RGD peptide to the amino end group of the PEG chain. In the proliferation test of human umbilical vein endothelial cells (HUVEC) on the modified PU matrix, the RGD-immobilized porous matrix showed enhanced viability of HUVEC as compared with an unmodified surface, demonstrating that the presence of RGD peptide promoted HUVEC proliferation. In addition, the RGD-immobilized PU porous matrix revealed higher cell viability than the RGD-immobilized PU film because of the porous structure with higher surface area, indicating an advantageous property of the porous matrix for HUVEC proliferation.
Choi
Won Sup
WS
Department of Molecular Science and Technology, Ajou University, 5 Wonchon, Yeongtong, Suwon 443-749, Korea.
Bae
Jin Woo
JW
Lim
Hye Ryeon
HR
Joung
Yoon Ki
YK
Park
Jong-Chul
JC
Kwon
Il Keun
IK
Park
Ki Dong
KD
D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000473:pathology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D001672:Biocompatible Materials; D009842:Oligopeptides; D010455:Peptides; D011140:Polyurethanes; D013778:Tetrazolium Salts; D013844:Thiazoles; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; C022616:thiazolyl blue