Development of a lateral flow colloidal gold immunoassay strip for the rapid detection of enrofloxacin residues.
A rapid immunochromatographic lateral flow test strip of competitive format has been developed using a gold-conjugated monoclonal antibody for the specific determination of enrofloxacin (ENR) residues in chicken muscles. For this purpose, a specific monoclonal antibody (mAb) for ENR was generated and characterized. The mAb showed negligible cross-reactivity with other related compounds. Using ENR standards prepared in chicken muscle extracts from 0 to 24.3 ng/mL (microg/kg), the method indicated that the detection limit of the test strip, as measured in a strip scanner, was as low as 0.138 microg/kg of ENR and the half-maximal inhibition concentration (IC(50)) was 0.935 microg/kg. For samples spiked at 10, 20, and 30 microg/kg, the recovery was between 85.3 and 96.1% and the coefficient of variation [CV (%)] was between 4.5 and 7.91%. Parallel analysis of muscle samples from chickens fed ENR showed good comparable results obtained from the test strip and LC-MS. Each test requires 5-10 min. The data indicate that the method has high sensitivity, specificity, and the advantages of simplicity and speed of performance. Therefore, the test strip provides a useful screening method for quantitative, semiquantitative, or qualitative detection of ENR residues in chicken muscles.
Zhao
Yinli
Y
The Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Zhang
Gaiping
G
Liu
Qingtang
Q
Teng
Man
M
Yang
Jifei
J
Wang
Jianhua
J
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D004350:Drug Residues / Q000032:analysis*; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000032:analysis*; D017979:Gold Colloid / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000737:chemistry; D011201:Poultry Diseases / Q000188:drug therapy*; D011934:Reagent Strips / Q000032:analysis*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D017979:Gold Colloid; D011934:Reagent Strips; D000077422:Enrofloxacin