title: Effect of interdomain linker length on an antagonistic folding-unfolding equilibrium between two protein domains.
abstract: Fusion of one protein domain with another is a common event in both evolution and protein engineering experiments. When insertion is at an internal site (e.g., a surface loop or turn), as opposed to one of the termini, conformational strain can be introduced into both domains. Strain is manifested by an antagonistic folding-unfolding equilibrium between the two domains, which we previously showed can be parameterized by a coupling free-energy term (DeltaG(X)). The extent of strain is predicted to depend primarily on the ratio of the N-to-C distance of the guest protein to the distance between ends of the surface loop in the host protein. Here, we test that hypothesis by inserting ubiquitin (Ub) into the bacterial ribonuclease barnase (Bn), using peptide linkers from zero to 10 amino acids each. DeltaG(X) values are determined by measuring the extent to which Co(2+) binding to an engineered site on the Ub domain destabilizes the Bn domain. All-atom, unforced Langevin dynamics simulations are employed to gain structural insight into the mechanism of mechanically induced unfolding. Experimental and computational results find that the two domains are structurally and energetically uncoupled when linkers are long and that DeltaG(X) increases with decreasing linker length. When the linkers are fewer than two amino acids, strain is so great that one domain unfolds the other. However, the protein is able to refold as dimers and higher-order oligomers. The likely mechanism is a three-dimensional domain swap of the Bn domain, which relieves conformational strain. The simulations suggest that an effective route to mechanical unfolding begins with disruption of the hydrophobic core of Bn near the Ub insertion site.
authors.affiliation: Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Cutler
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Mills
authors.forename: David J
authors.initials: DJ
authors.lastname: Lubin
authors.forename: Lillian T
authors.initials: LT
authors.lastname: Chong
authors.forename: Stewart N
authors.initials: SN
authors.lastname: Loh
mesh_terms: D001426:Bacterial Proteins; D002413:Cations, Divalent / Q000378:metabolism; D002942:Circular Dichroism; D003035:Cobalt / Q000378:metabolism; D003198:Computer Simulation; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics; D025801:Ubiquitin / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; D003035:Cobalt; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease