Effect of intravitreal plasmin on vitreous removal through a 25-gauge cutting system in the rabbit in vivo.
OBJECTIVE
Intravitreal plasmin creates a posterior vitreous detachment, but may also liquefy the vitreous. This study measures the rate of vitreous removal from rabbit eyes after plasmin injection in vivo.


METHODS
Intravitreal injections of 150 IU hyaluronidase (n = 5), 0.5 activity units (AU, n = 6) or 0.9 AU of streptokinase-activated human plasmin (n = four groups of 6) in 0.1 ml were performed in rabbits, the fellow eyes received 0.1 ml BSS. After 30 min (hyaluronidase), 30 min, 4 h, 12 h or 24 h (0.9 AU plasmin) or 24 h (0.5 AU plasmin), 1 ml of vitreous was removed from each eye without infusion, using a 25-gauge cutter and a standardized protocol. Animals were sacrificed after surgery.


RESULTS
Compared to fellow eyes, the average rate of vitreous removal was increased by hyaluronidase by 68.9 +/- 6.3% (p < 0.05) and by 0.5 AU plasmin (24 h) by 26.8 +/- 3.3% (p < 0.05). 0.9 AU of plasmin increased removal rates by 0.8 +/- 10% (n.s.), 15.4 +/- 6.3% (p < 0.05), 40.3 +/- 3.1% (p < 0.05), and 71.9 +/- 32.4% (p < 0.05) after 30 min, 4 h, 12 h and 24 h incubation respectively. The ratios of removal rates of treated/control eyes in the 0.9 AU groups showed a linear correlation with incubation time (r = 0.783, p < 0.0001).


CONCLUSIONS
Intravitreal plasmin increases the rate of vitreous removal in rabbits.
Hermel
Martin
M
Department of Ophthalmology, RWTH Aachen University, Pauwelsstrasse 30, 52057, Aachen, Germany. mah30@gmx.de
Prenner
Jonathan
J
Alabdulrazzak
Motaz
M
Dailey
Wendy
W
Hartzer
Michael
M
D000818:Animals; D005341:Fibrinolysin / Q000008:administration & dosage*; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D006821:Hyaluronoglucosaminidase / Q000008:administration & dosage; D007267:Injections; D008866:Microsurgery / Q000379:methods*; D011817:Rabbits; D014821:Vitrectomy / Q000295:instrumentation / Q000379:methods*; D014822:Vitreous Body / Q000187:drug effects*
D005343:Fibrinolytic Agents; D006821:Hyaluronoglucosaminidase; D005341:Fibrinolysin