title: Chaperone-like activity of heme group against amyloid-like fibril formation by hen egg ovalbumin: possible mechanism of action.
abstract: It is well accepted that porphyrins related to heme, are effectively able to act as pharmaceutical. For instance, they were found to interfere with the aggregation of beta-amyloid peptide. In present study, the influence of heme concentration on the amyloid-type aggregation of ovalbumin was investigated. We provided experimental evidence of heme's prevention of ovalbumin aggregation in the heat-denaturation process using spectroscopic measurements. Different types of interactions were suggested to be involved in heme-ovalbumin amyloid communication. Since the consequence of heme-attenuated fibrillogenesis is yet to be identified, additional data on chaperoning role of heme may help us to manage amyloid aggregation processes.
authors.affiliation: Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, P.O. Box 67145-1673, Kermanshah University of Medical Sciences, Kermanshah, Iran. rkhodarahmi@mbrc.ac.ir
authors.forename: Reza
authors.initials: R
authors.lastname: Khodarahmi
authors.forename: Hosnieh
authors.initials: H
authors.lastname: Soori
authors.forename: Seyyed Arash
authors.initials: SA
authors.lastname: Karimi
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D003224:Congo Red; D004121:Dimethyl Sulfoxide; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005453:Fluorescence; D006418:Heme / Q000378:metabolism*; D006358:Hot Temperature; D018832:Molecular Chaperones / Q000378:metabolism*; D010047:Ovalbumin / Q000378:metabolism*; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D014270:Trifluoroethanol
chemical_list: D000682:Amyloid; D018832:Molecular Chaperones; D003224:Congo Red; D006418:Heme; D014270:Trifluoroethanol; D010047:Ovalbumin; D004121:Dimethyl Sulfoxide