Periodic assembly of nanospecies on repetitive DNA sequences generated on gold nanoparticles by rolling circle amplification.
Periodical assembly of nanospecies is desirable for the construction of nanodevices. We provide a protocol for the preparation of a gold nanoparticle (AuNP)/DNA scaffold on which nanospecies can be assembled in a periodical manner. AuNP/DNA scaffold is prepared by growing long single-stranded DNA (ssDNA) molecules (typically hundreds of nanometers to a few microns in length) on AuNPs via rolling circle amplification (RCA). Since these long ssDNA molecules contain many repetitive sequence units, complementary DNA-attached nanospecies can be assembled through specific hybridization in a controllable and periodical manner.
Zhao
Weian
W
Department of Chemistry, McMaster University, Hamilton, Ontario, Canada.
Brook
Michael A
MA
Li
Yingfu
Y
D004277:DNA, Single-Stranded / Q000138:chemical synthesis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid
D004277:DNA, Single-Stranded; D006046:Gold