title: Combination of CCR1 mRNA, MCP1, and CA125 measurements in peripheral blood as a diagnostic test for endometriosis.
abstract: This study investigated the possible use of CCR1 mRNA measurement in peripheral blood leukocytes in combination with measurements of monocyte chemotactic protein-1 (MCP-1) and CA125 protein in serum as a diagnostic test for endometriosis.The expression of CCR1 mRNA in peripheral blood leukocytes was measured by quantitative real-time polymerase chain reaction. MCP-1 and CA125 levels in serum were determined by ELISA and ECLIA.The ratio of CCR1/HPRT mRNA in peripheral blood of patients with endometriosis and adenomyosis was significantly elevated compared with women without endometriosis. Additionally, serum levels of MCP-1 and CA125 were significantly higher in patients with endometriosis. This method showed a sensitivity of 92.2%, a specificity of 81.6%, a negative predictive value of 83.3%, a positive predictive value of 92.3%, a likelihood ratio of a positive test result of 5.017, and a likelihood ratio of a negative test result of 0.096 to predict the presence or absence of endometriosis.The results imply the potential use of CCR1 mRNA, MCP-1, and CA125 protein measurements for the diagnosis or exclusion of endometriosis.
authors.affiliation: Department of Gynecology and Obstetrics, University of Schleswig-Holstein, Luebeck, Germany.
authors.forename: Admir
authors.initials: A
authors.lastname: Agic
authors.forename: Schima
authors.initials: S
authors.lastname: Djalali
authors.forename: Monika M
authors.initials: MM
authors.lastname: Wolfler
authors.forename: Gulden
authors.initials: G
authors.lastname: Halis
authors.forename: Klaus
authors.initials: K
authors.lastname: Diedrich
authors.forename: Daniela
authors.initials: D
authors.lastname: Hornung
mesh_terms: D018394:CA-125 Antigen / Q000097:blood*; D018932:Chemokine CCL2 / Q000097:blood*; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D012333:RNA, Messenger / Q000097:blood; D054389:Receptors, CCR1 / Q000097:blood* / Q000235:genetics; D012189:Retrospective Studies
chemical_list: D018394:CA-125 Antigen; C111835:CCL2 protein, human; C515300:CCR1 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger; D054389:Receptors, CCR1