Highly selective detection of 5-methylcytosine using photochemical ligation.
We report the nonenzymatic detection of 5-methylcytosine by using template-directed photoligation through 5-cyanovinyl-2'-deoxyuridine ((C)U) with high selectivity and present a new methylation detection method using a photoligation-based DNA chip assay.
Ogino
Masayuki
M
Japan Science and Technology Agency, Ishikawa, 923-1211, Japan.
Taya
Yuuta
Y
Fujimoto
Kenzo
K
D044503:5-Methylcytosine / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D015342:DNA Probes / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010777:Photochemistry
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D044503:5-Methylcytosine; D004247:DNA