Murine and human cathepsin B exhibit similar properties: possible implications for drug discovery.
Validation of drug targets and subsequent preclinical studies are usually carried out on animal disease models, with mouse being the most commonly used. However, results from mouse models cannot always be directly related to human disease. Major discrepancies between the properties of murine and human variants were observed during the evaluation of compounds targeting cathepsins S and K. It is important, therefore, to know whether similar differences exist between murine and human cathepsin B. Thus, both enzymes were expressed and biochemically characterized. The enzymes exhibited similar biochemical properties, indicating that cathepsin B transgenic mouse models could be useful for studying its role in human pathologies.
Caglic
Dejan
D
Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
Kosec
Gregor
G
Bojic
Lea
L
Reinheckel
Thomas
T
Turk
Vito
V
Turk
Boris
B
D000595:Amino Acid Sequence; D000818:Animals; D002401:Cathepsin B / Q000737:chemistry* / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D015195:Drug Design; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D002401:Cathepsin B