The promoting angiogenesis and anti-inflammation effect of scutellarin on polyglycolic acid scaffold of balb/c mice model.
Engineering artificial implantable tissues require rapid induction of angiogenesis to meet the significant oxygen and nutrient demands of the cell during tissue repair. In this study, we investigated the role of a Chinese medicine, scutellarin (1), in the tissue engineering scaffold of balb/c mice model. The trial groups of balb/c mice were given an intraperitoneal injection of 1 at 20, 60, and 100 mg/kg per ml, respectively. The implanted samples were retrieved at 1 week and 1 month post transplantation. Angiogenesis response in polyglycolic acid (PGA) scaffold was evaluated by histopathological and immunohistochemical methods. The expression levels of vascular endothelial growth factor (VEGF) were determined by RT-PCR. The results showed that the density of neocapillaries in the PGA was enhanced by basic fibroblast growth factor (bFGF) and 1 at 1 week. At 1 month, only 60 and 100 mg/kg per ml 1 groups continuingly kept significant neocapillaries. The inflammatory cells were significantly less in the 100 mg/kg per ml 1 group in comparison with bFGF group and negative group at 1 week and 1 month. Our results indicated that 1 not only promoted angiogenesis in the PGA scaffold, but also inhibited the host inflammatory response to the xenogenic materials.
Ma
Jing-Yuan
JY
Department of Oral Medicine, School of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Affiliated The Ninth People's Hospital, Shanghai, China.
Jiang
Wei-Wen
WW
Zhou
Zeng-Tong
ZT
Li
Jing-Min
JM
Wang
Hai-Yan
HY
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D047310:Apigenin / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D005965:Glucuronates / Q000737:chemistry* / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011100:Polyglycolic Acid / Q000737:chemistry; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001672:Biocompatible Materials; D005965:Glucuronates; D042461:Vascular Endothelial Growth Factor A; C484876:scutellarin; D011100:Polyglycolic Acid; D012313:RNA; D047310:Apigenin