Vascularization of engineered teeth.
The implantation of cultured dental cell-cell re-associations allows for the reproduction of fully formed teeth, crown morphogenesis, epithelial histogenesis, mineralized dentin and enamel deposition, and root-periodontium development. Since vascularization is critical for organogenesis and tissue engineering, this work aimed to study: (a) blood vessel formation during tooth development, (b) the fate of blood vessels in cultured teeth and re-associations, and (c) vascularization after in vivo implantation. Ex vivo, blood vessels developed in the dental mesenchyme from the cap to bell stages and in the enamel organ, shortly before ameloblast differentiation. In cultured teeth and re-associations, blood-vessel-like structures remained in the peridental mesenchyme, but never developed into dental tissues. After implantation, both teeth and re-associations became revascularized, although later in the case of the re-associations. In implanted re-associations, newly formed blood vessels originated from the host, allowing for their survival, and affording conditions organ growth, mineralization, and enamel secretion.
Nait Lechguer
A
A
INSERM UMR 595, Faculté de Médecine, 11, rue Humann, 67085 Strasbourg cedex, France.
Kuchler-Bopp
S
S
Hu
B
B
Haïkel
Y
Y
Lesot
H
H
D000565:Ameloblasts / Q000502:physiology; D000566:Amelogenesis / Q000502:physiology; D000818:Animals; D001808:Blood Vessels / Q000254:growth & development; D002454:Cell Differentiation / Q000502:physiology; D024141:Collagen Type IV / Q000032:analysis; D003810:Dentinogenesis / Q000502:physiology; D004658:Enamel Organ / Q000254:growth & development; D004848:Epithelium / Q000254:growth & development; D008648:Mesoderm / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D009024:Morphogenesis / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009805:Odontogenesis / Q000502:physiology*; D009924:Organ Culture Techniques; D010519:Periodontium / Q000254:growth & development; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D023822:Tissue Engineering; D014070:Tooth / Q000098:blood supply* / Q000637:transplantation; D014074:Tooth Calcification / Q000502:physiology; D019228:Tooth Crown / Q000254:growth & development; D014083:Tooth Germ / Q000254:growth & development; D014092:Tooth Root / Q000254:growth & development; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000032:analysis
D024141:Collagen Type IV; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D040301:Vascular Endothelial Growth Factor Receptor-2