title: Sodium hydrosulfide alleviates pulmonary artery collagen remodeling in rats with high pulmonary blood flow.
abstract: This study aimed to explore the effect of sodium hydrosulfide (NaHS) on pulmonary artery collagen remodeling in rats with high pulmonary blood flow. Thirty-two Sprague-Dawley rats were randomly divided into a sham group, shunt group, sham + NaHS (an H2S donor) group, and shunt + NaHS group. After 11 weeks of shunting, mean pulmonary artery pressure (MPAP), relative median area (RMA) of pulmonary arteries, H2S concentration in lung tissues, plasma endothelin-1 (ET-1) levels, and ET-1 mRNA in lung tissues were investigated. Collagen I and collagen III were evaluated by immunohistochemistry. Hydroxyproline assay and Sirius-red staining were performed. Matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metalloproteinase-1 (TIMP-1), and connective tissue growth factor (CTGF) were evaluated by immunohistochemistry. After 11 weeks of shunting, rats showed a significant pulmonary hypertension and pulmonary artery collagen remodeling in association with a decrease in lung tissue H2S content. After NaHS treatment for 11 weeks, lung tissue H(2)S content was increased, whereas MPAP was attenuated and RMA was reduced. Meanwhile, pulmonary artery collagen I and collagen III protein expressions of intra-acinar pulmonary arteries were inhibited, but MMP-13/TIMP-1 ratio was augmented with a decreased plasma ET-1 content and lung tissue ET-1mRNA and CTGF expressions. The downregulation of H(2)S is involved in the development of pulmonary artery collagen remodeling induced by high pulmonary blood flow.
authors.affiliation: Department of Pediatrics, Peking University First Hospital and Key Laboratory of Molecular Cardiology, Ministry of Education, Xi-An Men Street No. 1, West District, Beijing, 100034, P.R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Junbao
authors.initials: J
authors.lastname: Du
authors.forename: Hongfang
authors.initials: H
authors.lastname: Jin
authors.forename: Bin
authors.initials: B
authors.lastname: Geng
authors.forename: Chaoshu
authors.initials: C
authors.lastname: Tang
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology*; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism*; D024061:Collagen Type III / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D006976:Hypertension, Pulmonary / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D011651:Pulmonary Artery / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D011669:Pulmonary Wedge Pressure / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D013440:Sulfides; C025451:sodium bisulfide