Astrocytic calcium signals induced by neuromodulators via functional metabotropic receptors in the ventral respiratory group of neonatal mice.
A controlled, periodic exchange of air between lungs and atmosphere requires a neuronal rhythm generated by a network of neurons in the ventral respiratory group (VRG) of the brainstem. Glial cells, e.g. astrocytes, have been shown to be supportive in stabilizing this neuronal activity in the central nervous system during development. In addition, a variety of neuromodulators including serotonin (5-HT), Substance P (SP), and thyrotropin-releasing hormone (TRH) stimulate respiratory neurons directly. If astrocytes in the VRG, like their neuronal neighbors, are also directly stimulated by neuromodulators, they might indirectly affect the respiratory neurons and consequently the respiratory rhythm. In the present study, we provide support for this concept by demonstrating expression of NK1-R, TRH-R, and 5-HT(2)-R in astrocytes of the VRG with immunohistochemistry. Additionally, we showed that the external application of the neuromodulators 5-HT, SP, and TRH activate calcium transients in VRG astrocytes. Consequently, we postulate that in the VRG of the neonatal mouse, neuromodulation by SP, TRH, and serotonin also involves astrocytic calcium signaling.
Härtel
Kai
K
Department of Neuro- and Sensory Physiology, Georg-August-University Göttingen, Göttingen, Germany.
Schnell
Christian
C
Hülsmann
Swen
S
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000235:genetics; D011189:Potassium Chloride / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D018040:Receptors, Neurokinin-1 / Q000235:genetics / Q000378:metabolism; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D012125:Respiratory Center / Q000166:cytology*; D018800:Thy-1 Antigens / Q000235:genetics; D044767:Ubiquitin-Protein Ligases; D000070584:Tripartite Motif Proteins
D002118:Calcium; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins; D018377:Neurotransmitter Agents; D009687:Nuclear Proteins; D011189:Potassium Chloride; D011956:Receptors, Cell Surface; D018040:Receptors, Neurokinin-1; D011985:Receptors, Serotonin; D018025:Receptors, Thyrotropin-Releasing Hormone; D018800:Thy-1 Antigens; D044767:Ubiquitin-Protein Ligases; C485184:enhanced green fluorescent protein; C489296:Trim27 protein, mouse; D000070584:Tripartite Motif Proteins