title: Hydrogen sulfide evokes neurite outgrowth and expression of high-voltage-activated Ca2+ currents in NG108-15 cells: involvement of T-type Ca2+ channels.
abstract: We investigated if stimulation of T-type Ca(2+) channels with sodium hydrosulfide (NaHS), a donor of hydrogen sulfide (H(2)S), could cause neuronal differentiation of NG108-15 cells. Like dibutyryl cyclic AMP (db-cAMP), treatment with NaHS at 1.5-13.5 mM for 16 h enhanced neurite outgrowth in a concentration-dependent manner. Synergistic neuritogenic effect was obtained in the cells stimulated with NaHS in combination with db-cAMP at subeffective concentrations. Exposure to NaHS or db-cAMP for 2 days resulted in enhancement of expression of high-voltage-activated currents consisting of N-, P/Q-, L- and also other types, but not of T-type currents. Mibefradil, a pan-T-type channel blocker, abolished the neuritogenesis induced by NaHS, but not by db-cAMP. The NaHS-evoked neuritogenesis was also completely blocked by pretreatment with BAPTA/AM, a chelator of intracellular Ca(2+), and by zinc chloride at a concentration known to selectively inhibit Ca(v)3.2 isoform of T-type Ca(2+) channels, but not Ca(v)3.1 or Ca(v)3.3. Further, L-ascorbate, recently proven to selectively inhibit Ca(v)3.2, abolished the neuritogenic effect of NaHS, but not db-cAMP. Our data thus demonstrate that NaHS/H(2)S is a novel inducer of neuronal differentiation in NG108-15 cells, as characterized by neuritogenesis and expression of high-voltage-activated currents, and suggest the involvement of T-type Ca(2+) channels, especially Ca(v)3.2.
authors.affiliation: Division of Pharmacology and Pathophysiology, Kinki University School of Pharmacy, Higashi-Osaka, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Nagasawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tarui
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yoshida
authors.forename: Fumiko
authors.initials: F
authors.lastname: Sekiguchi
authors.forename: Maho
authors.initials: M
authors.lastname: Matsunami
authors.forename: Ai
authors.initials: A
authors.lastname: Ohi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Fukami
authors.forename: Seiji
authors.initials: S
authors.lastname: Ichida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nishikawa
authors.forename: Atsufumi
authors.initials: A
authors.lastname: Kawabata
mesh_terms: D003994:Bucladesine / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000096:biosynthesis* / Q000187:drug effects*; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000502:physiology; D020865:Calcium Channels, P-Type / Q000187:drug effects / Q000502:physiology; D020747:Calcium Channels, T-Type / Q000502:physiology*; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D017967:Zinc Compounds / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D020865:Calcium Channels, P-Type; D020747:Calcium Channels, T-Type; D002614:Chelating Agents; D002712:Chlorides; D013439:Sulfhydryl Reagents; D017967:Zinc Compounds; D004533:Egtazic Acid; D003994:Bucladesine; C016837:zinc chloride; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D004229:Dithiothreitol; D006862:Hydrogen Sulfide