Augmentation of angiogenic and endothelial associated gene expression by EDM50 in human chorion-derived stem cells.
The objective of this study was to investigate the angiogenic potential of human chorion-derived stem cells (CDSC) cultured in medium containing bFGF and VEGF (EDM50). Total RNA was extracted from cells cultured in FD+10% FBS and EDM50. Quantitative RT-PCR was carried out to score the differential mRNA expression of genes involve in angiogenesis and endothelial differentiation. Our finding demonstrated that all angiogenic and endothelial associated genes were expressed higher in EDM50. Expression level of ANG-1, eNOS and VEGFR2 were significantly higher in EDM50 compared to FD+10% FBS. Our results suggested that human CDSC cultured in EDM50 can be used for angiogenesis purpose in regenerative medicine.
Manzor
N F Mohd
NF
Department of Pathology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
Chua
K H
KH
Tan
G C
GC
Tan
A E
AE
Abdul Rahman
H
H
D002823:Chorion / Q000378:metabolism*; D004727:Endothelium / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D005810:Multigene Family; D018919:Neovascularization, Physiologic / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2