A scanning electron microscopic study of in vivo tissue engineered respiratory epithelium in sheep.
Normal tracheal mucociliary clearance is the key to maintaining the health and defense of respiratory airway. Therefore the present of cilia and mucous blanket are important for tracheal epithelium to function effectively. In the present study, we prepared a tissue engineered respiratory epithelium construct (TEREC) made of autologous respiratory epithelium cells, fibroblast and fibrin from sheep owns blood which replaced a created tracheal mucosal defect. Scanning electron microscopy (SEM) showed encouraging result where immature cilia were present on the surface of TEREC. This result indicates that engineered respiratory epithelium was able to function as normal tissue.
Heikal
M Y Mohd
MY
Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
Aminuddin
B S
BS
Jeevanan
J
J
Chen
H C
HC
Sharifah
S
S
Ruszymah
B H I
BH
D000818:Animals; D002923:Cilia / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology*; D004848:Epithelium / Q000502:physiology*; D005337:Fibrin / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D010865:Pilot Projects; D012137:Respiratory System / Q000166:cytology*; D034561:Sheep, Domestic; D023822:Tissue Engineering; D014132:Trachea / Q000166:cytology* / Q000502:physiology; D014182:Transplantation, Autologous
D005337:Fibrin