Assessment of effects of a Cordia salicifolia extract on the radiolabeling of blood constituents and on the morphology of red blood cells.
Effects of a Cordia salicifolia (porangaba) extract on the labeling of blood cells (BCs) with technetium-99m ((99m)Tc) and on the morphology of red BCs were evaluated. Labeling of cellular and molecular structures with (99m)Tc depends on a reducing agent. Some physical characteristics, as visible absorbance spectrum, electric conductivity, and refractive index of this porangaba extract, were also determined. Blood samples from Wistar rats were incubated with porangaba extract or with 0.9% NaCl (control). Labeling of blood constituents with (99m)Tc was performed. Plasma (P) and BCs, both soluble (SF-P and SF-BC) and insoluble (IF-P and IF-BC) fractions, were separated. The radioactivity in each fraction was counted, and the percentage of radioactivity incorporated (%ATI) was calculated. Blood smears were prepared, fixed, and stained, and the morphology of the red BCs was evaluated. Data showed an absorbance peak at 480 nm and electric conductibility and refractive index concentration-dependent. Porangaba extract decreased significantly (P < .05) the BC, IF-P, and IF-BC %ATI, and no modifications were verified on the shape of red BCs. Analysis of the results reveals that some physical parameters could be useful to aid in characterizing the extract studied. Moreover, it is possible that chemical compounds of this extract could have chelating/redox actions or be capable of binding to plasma and/or cellular proteins.
Frydman
Jacques Natan Grinapel
JN
Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
Rocha
Vanessa Camara
VC
Benarroz
Monica Oliveira
MO
Rocha
Gabrielle Souza
GS
Pereira
Márcia Oliveira
MO
de Souza da Fonseca
Adenilson
A
Bernardo-Filho
Mario
M
D000818:Animals; D001773:Blood Cells / Q000187:drug effects*; D002352:Carrier Proteins; D031213:Cordia; D004553:Electric Conductivity; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D007553:Isotope Labeling; D008297:Male; D008565:Membrane Proteins; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D010949:Plasma / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012031:Refractometry; D013053:Spectrophotometry; D013667:Technetium
D002352:Carrier Proteins; D008565:Membrane Proteins; D010936:Plant Extracts; C076239:P protein, mouse; D013667:Technetium