title: CD146(+) bone marrow osteoprogenitors increase in the advanced stages of primary myelofibrosis.
abstract: CD146(+) bone marrow stromal cells have been recently recognized as clonogenic osteoprogenitors able to organize a complete hematopoietic microenvironment. In this study we used immunohistochemical analysis to investigate the contribution of CD146(+) bone marrow osteoprogenitors to the stromal remodeling occurring in the different stages of primary myelofibrosis. We found that CD146(+) cells sited at the abluminal side of the bone marrow vessels and branching among hematopoietic cells significantly increased in the advanced stages of primary myelofibrosis (p<0.001), paralleling the extent of fibrosis (rho=0.916, p<0.0001) and the microvascular density (r=0.883, p<0.0001). Coherently with a mural cell function, such cells also displayed smooth-muscle actin expression. Our data providing evidence of CD146(+) cell involvement in bone marrow stromal changes occurring in primary myelofibrosis are consistent with the capability of these cells to participate in fiber deposition, angiogenesis, and bone formation. They could also represent rationale for new therapies targeting the bone marrow stroma in primary myelofibrosis.
authors.affiliation: Department of Human Pathology, University of Palermo, Palermo, Italy. tripodo@unipa.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Tripodo
authors.forename: Andrea
authors.initials: A
authors.lastname: Di Bernardo
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Ternullo
authors.forename: Carla
authors.initials: C
authors.lastname: Guarnotta
authors.forename: Rossana
authors.initials: R
authors.lastname: Porcasi
authors.forename: Sabrina
authors.initials: S
authors.lastname: Ingrao
authors.forename: Umberto
authors.initials: U
authors.lastname: Gianelli
authors.forename: Emanuela
authors.initials: E
authors.lastname: Boveri
authors.forename: Emilio
authors.initials: E
authors.lastname: Iannitto
authors.forename: Giovanni
authors.initials: G
authors.lastname: Franco
authors.forename: Ada Maria
authors.initials: AM
authors.lastname: Florena
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism*; D051929:CD146 Antigen / Q000378:metabolism*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D055728:Primary Myelofibrosis / Q000378:metabolism* / Q000473:pathology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D051929:CD146 Antigen