Stimulative effects of light and a temperature downshift on transcriptional expressions of developmentally regulated genes in the initial stages of fruiting-body formation of the basidiomycetous mushroom Lentinula edodes.
Quantitative reverse transcription PCR experiments were done to analyze the effects of light and a temperature downshift on transcriptional expressions of a variety of developmentally regulated genes in the initial stages of fruiting-body formation of Lentinula edodes wild-type strain FMC2. It was revealed that both proper light conditions and a temperature downshift are required for high levels of transcriptions of the genes and the formation of fruiting bodies. In L. edodes MIL-LEW-M13-1 strain, which has been reported previously to be capable of forming fruiting bodies without a low-temperature treatment, the expressions of the developmentally regulated genes were enhanced by light exposure.
Nakazawa
Takehito
T
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. nakazawa.t.ac@m.titech.ac.jp
Miyazaki
Yasumasa
Y
Kaneko
Shinya
S
Shishido
Kazuo
K
D048690:Fruiting Bodies, Fungal / Q000254:growth & development* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008027:Light; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020942:Shiitake Mushrooms / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
D005656:Fungal Proteins