title: Binding of individual Bacillus thuringiensis Cry proteins to the olive moth Prays oleae (Lepidoptera: Yponomeutidae).
abstract: The microlepidopteran Prays oleae is one of the main insect pests causing significant crop losses in the Mediterranean olive groves. Bacillus thuringiensis based insecticides are being successfully used to minimize the impact of the second and third generations of this pest. However, because of its very small size and difficulty of rearing, very few studies have been carried out to determine the potency and mode of action of B. thuringiensis Cry proteins in this insect. In this study, Cry1Ac, Cry1Ca, and Cry1Fa proteins were shown to be toxic to third instar larvae of P. oleae. Furthermore, binding assays with (125)I-Cry1Ac and brush border membrane vesicles from midguts of last-instar larvae showed specific binding sites for Cry1Ac that are shared, with low affinity, by Cry1Ca and Cry1Fa.
authors.affiliation: Departament de Genètica, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
authors.forename: Carmen Sara
authors.initials: CS
authors.lastname: Hernández-Rodríguez
authors.forename: Sergio
authors.initials: S
authors.lastname: Pérez-Guerrero
authors.forename: Hani K
authors.initials: HK
authors.lastname: Aldebis
authors.forename: Enrique
authors.initials: E
authors.lastname: Vargas-Osuna
authors.forename: Juan
authors.initials: J
authors.lastname: Ferré
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D004731:Endotoxins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D009036:Moths / Q000254:growth & development / Q000378:metabolism*; D010572:Pest Control, Biological
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis