title: Detection and characterization of waterborne gastroenteritis viruses in urban sewage and sewage-polluted river waters in Caracas, Venezuela.
abstract: The detection and molecular characterization of pathogenic human viruses in urban sewage have been used extensively to derive information on circulating viruses in given populations throughout the world. In this study, a similar approach was applied to provide an overview of the epidemiology of waterborne gastroenteritis viruses circulating in urban areas of Caracas, the capital city of Venezuela in South America. Dry season sampling was conducted in sewers and in a major river severely polluted with urban sewage discharges. Nested PCR was used for detection of human adenoviruses (HAds), while reverse transcription plus nested or seminested PCR was used for detection of enteroviruses (HuEVs), rotaviruses (HRVs), noroviruses (HuNoVs), and astroviruses (HAstVs). HRVs were fully characterized with genotype-specific primers for VP4 (genotype P), VP7 (genotype G), and the rotavirus nonstructural protein 4 (NSP4). HuNoVs and HAstVs were characterized by sequencing and phylogenetic analysis. The detection rates of all viruses were >or=50%, and all sampling events were positive for at least one of the pathogenic viruses studied. The predominant HRV types found were G1, P[8], P[4], and NSP4A and -B. Genogroup II of HuNoVs and HAstV type 8 were frequently detected in sewage and sewage-polluted river waters. This study reveals relevant epidemiological data on the distribution and persistence of human pathogenic viruses in sewage-polluted waters and addresses the potential health risks associated with transmission of these viruses through water-related environmental routes.
authors.affiliation: Laboratorio de Biología de Virus, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
authors.forename: J
authors.initials: J
authors.lastname: Rodríguez-Díaz
authors.forename: L
authors.initials: L
authors.lastname: Querales
authors.forename: L
authors.initials: L
authors.lastname: Caraballo
authors.forename: E
authors.initials: E
authors.lastname: Vizzi
authors.forename: F
authors.initials: F
authors.lastname: Liprandi
authors.forename: H
authors.initials: H
authors.lastname: Takiff
authors.forename: W Q
authors.initials: WQ
authors.lastname: Betancourt
mesh_terms: D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D045483:Rivers / Q000821:virology*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000821:virology*; D014505:Urban Population; D014687:Venezuela; D014764:Viral Proteins / Q000235:genetics; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D012722:Sewage; D014764:Viral Proteins