Unusual amplification pattern by SSP HLA-B typing led to discovery of a novel HLA-C allele.
We report here the unique sequence of a novel human leucocyte antigen (HLA)-C allele resulted in amplification of a product with a primer pair used in sequence-specific primer (SSP) HLA-B typing, which led subsequently to identification of a new allele of Cw*0339 by sequence-based typing method. This new allele is closely related to Cw*031101 with only two nucleotide changes from T to G at position 97, and T to C at position 105 in the exon 2 region of HLA-C locus. The first nucleotide substitution causes an amino acid alteration from tyrosine to aspartic acid at amino acid residue 9 (Y to D), while the second one keeps alanine at residue 11 unchanged. Most interestingly, the nucleotide change from T to G at position 97 makes it identical to the 3' end sequence of one SSP of the commercial HLA-B SSP typing kit we were using. In this specific case, it resulted in amplification of a product of HLA-C gene instead of B gene.
Miao
K R
KR
First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Pan
Q Q
QQ
Wu
H X
HX
Zhou
X Y
XY
Pan
M
M
Xue
M
M
Fan
S
S
Wang
X Y
XY
Zhao
X
X
Tang
R C
RC
Wang
C Y
CY
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015235:HLA-B Antigens; D015236:HLA-C Antigens