Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis.
Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not been formally examined. In this paper, we have investigated the contribution of the mannose receptor, Dectin-1, and complement receptor 3; and we have demonstrated that Dectin-1 is the main non-opsonic receptor involved in fungal uptake. However, both Dectin-1 and complement receptor 3 were found to accumulate at the site of uptake, while mannose receptor accumulated on C. albicans phagosomes at later stages. These results suggest a potential role for MR in phagosome sampling; and, accordingly, MR deficiency led to a reduction in TNF-alpha and MCP-1 production in response to C. albicans uptake. Our data suggest that pattern recognition receptors sample the fungal phagosome in a sequential fashion.
Heinsbroek
Sigrid E M
SE
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
Taylor
Philip R
PR
Martinez
Fernando O
FO
Martinez-Pomares
Luisa
L
Brown
Gordon D
GD
Gordon
Siamon
S
D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000276:immunology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D010588:Phagosomes; D011956:Receptors, Cell Surface / Q000276:immunology; D051192:Receptors, Pattern Recognition / Q000276:immunology*; D013997:Time Factors; D000099181:Dectin-1
D037181:Lectins, C-Type; D016177:Macrophage-1 Antigen; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D051192:Receptors, Pattern Recognition; D000099181:Dectin-1