Peptide fragmentation induced by radicals at atmospheric pressure.
A novel ion dissociation technique, which is capable of providing an efficient fragmentation of peptides at essential atmospheric pressure conditions, is developed. The fragmentation patterns observed often contain c-type fragments that are specific to electron capture dissociation/electron transfer dissociation (ECD/ETD), along with the y-/b-type fragments that are specific to collision-activated dissociation (CAD). In the presented experimental setup, ion fragmentation takes place within a flow reactor located in the atmospheric pressure region between the ion source and the mass spectrometer. According to a proposed mechanism, the fragmentation results from the interaction of ESI-generated analyte ions with the gas-phase radical species produced by a corona discharge source.
Vilkov
Andrey N
AN
MassTech, Inc., Columbia, MD, USA. andrey_vilkov@apmaldi.com
Laiko
Victor V
VV
Doroshenko
Vladimir M
VM
D001274:Atmospheric Pressure; D006358:Hot Temperature; D017665:Hydroxyl Radical / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007700:Kinetics; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D007477:Ions; D010446:Peptide Fragments; D017665:Hydroxyl Radical