Salvia miltiorrhiza depresses plasminogen activator inhibitor-1 production through inhibition of angiotensin II.
The purpose of this study was to investigate the effect of Salvia miltiorrhiza on the production of plasminogen activator inhibitor-1(PAI-1) induced by angiotensin II (Ang II) in renal mesangial cells. Rat mesangial cells were exposed to 100 nM Ang II. Meanwhile, different concentrations of Salvia miltiorrhiza injection were added to Mesangial Cells. PAI-1 mRNA was measured by semi-quantification reverse transcriptase polymerase chain reaction (RT-PCR) and PAI-1 protein by Western blotting. ELISA was used to detect the expression of transforming growth factor beta(1) (TGF-beta(1)) in serum free MEM medium. The level of cellular reactive oxygen species (ROS) was measured by confocal laser scanning microscopy. Salvia miltiorrhiza notably attenuated expression of PAI-1 induced by Ang II in a concentration-dependent manner. Meanwhile, it suppressed the production of TGF-beta(1) and cellular ROS in mesangial cells. These effects were due to Salvia miltiorrhiza's ability of inhibiting the activities of angiotensin II. Therefore, Salvia miltiorrhiza can be used to retard progression of glomerular sclerosis.
Yuan
Jun
J
Department of Nephrology, Affiliated Hospital of Hubei Traditional Chinese Medical College, Wuchang, Wuhan City, China. yjun_92@126.com
Wang
Xiaoqin
X
Chen
Taohou
T
Chen
Gang
G
Lu
Yanfang
Y
D000804:Angiotensin II / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D050527:Mesangial Cells / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000378:metabolism
D010936:Plant Extracts; D017395:Plasminogen Activator Inhibitor 1; D017382:Reactive Oxygen Species; D016212:Transforming Growth Factor beta; D000804:Angiotensin II