title: High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.
abstract: We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.
authors.affiliation: Department of Chemistry & Biochemistry, David Geffen School of Medicine, UCLA, Los Angeles, California 90024, USA. gopal@chem.ucla.edu
authors.forename: Gopal
authors.initials: G
authors.lastname: Iyer
authors.forename: Xavier
authors.initials: X
authors.lastname: Michalet
authors.forename: Yun-Pei
authors.initials: YP
authors.lastname: Chang
authors.forename: Fabien F
authors.initials: FF
authors.lastname: Pinaud
authors.forename: Stephanie E
authors.initials: SE
authors.lastname: Matyas
authors.forename: Gregory
authors.initials: G
authors.lastname: Payne
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
mesh_terms: D006241:Haptens / Q000737:chemistry*; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D045663:Quantum Dots
chemical_list: D006241:Haptens; D007128:Immunoglobulin Fragments; D011506:Proteins