title: Application of a Reverse Line Blot hybridisation assay for the species-specific identification of cyathostomins (Nematoda, Strongylida) from benzimidazole-treated horses in the Slovak Republic.
abstract: Five horse farms located in eastern Slovakia were investigated for the presence of benzimidazole-resistant strongyles by faecal egg count reduction test and egg hatch assay. Coprocultures were prepared for each farm from faecal samples taken pre- and post-treatment and harvested larvae were molecularly examined with a Reverse Line Blot assay. Faecal egg count reduction values ranged from 0 to 52.5% and all farms were positive for benzimidazole-resistant cyathostomins. Seven benzimidazole-resistant cyathostomin species were molecularly identified on farms before and also after treatment. These data demonstrate that resistance to benzimidazoles is well established in cyathostomin populations from horse farms in the Slovak Republic and that the molecular assay was able to determine the species-specific distribution of resistant cyathostomins under field conditions.
authors.affiliation: Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001 Kosice, Slovak Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Cernanská
authors.forename: Barbara
authors.initials: B
authors.lastname: Paoletti
authors.forename: Ivica
authors.initials: I
authors.lastname: Králová-Hromadová
authors.forename: Raffaella
authors.initials: R
authors.lastname: Iorio
authors.forename: Patrícia
authors.initials: P
authors.lastname: Cudeková
authors.forename: Piermarino
authors.initials: P
authors.lastname: Milillo
authors.forename: Donato
authors.initials: D
authors.lastname: Traversa
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D001562:Benzimidazoles / Q000627:therapeutic use*; D006734:Horse Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D006736:Horses; D018154:Slovakia / Q000453:epidemiology; D013045:Species Specificity; D017183:Strongylida / Q000145:classification*; D017206:Strongylida Infections / Q000188:drug therapy / Q000453:epidemiology / Q000662:veterinary*; D020798:Two-Hybrid System Techniques / Q000662:veterinary*
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; C031000:benzimidazole