title: [BFGF entraped in cationic liposomes as adjuvant: optimization of technical parameters].
abstract: OBJECTIVE
  To test the immune efficiency of bFGF entraped in cationic liposomes as adjuvant in vivo.
  
  
  METHODS
  The technical parameters on encapsulation were tested in each step to gain high encapsulation efficiencies, which included lipid composition, weight ratio of protein and lipids, liposome extrusion, and different conditions of freeze-thawing. The bFGF in cationic liposome, Freund's adjuvant, or PBS were injected (four times) to the four-week old Balb/c mice to test the immune responses. The serum antibody was measured by ELISA 13 days after each injection.
  
  
  RESULTS
  Maximal encapsulation efficiency (about 50%) was achieved through optimized technical parameters. Cationic liposome demonstrated satisfied immune efficiency as adjuvant.
  
  
  CONCLUSIONS
  Cationic liposome is a safe and effective immunological adjuvant.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Zhong
authors.forename: Shuang-lin
authors.initials: SL
authors.lastname: Mao
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Guang-li
authors.initials: GL
authors.lastname: Yang
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Hou
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry / Q000276:immunology; D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000276:immunology*; D005620:Freund's Adjuvant / Q000276:immunology; D007114:Immunization; D008081:Liposomes / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D002412:Cations; D004337:Drug Carriers; D008081:Liposomes; D016222:Fibroblast Growth Factor 2; D005620:Freund's Adjuvant