title: A fibrin gel carrier system for islet transplantation into kidney subcapsule.
abstract: Islet transplantation is a promising therapeutic option for type 1 diabetes, and actively performed in the clinic as well as in the animal experiments. For the rodent experiments, islet transplantation into kidney subcapsule is widely used to assess islet quality, however, it is often difficult to do using a polyethylene tubing and fine needle because of inherent dead volume of needle and stickiness of the tubing to islets. This problem makes it difficult to interpret the physiological response to different islet doses. Here, we developed a simple fibrin gel carrier system for islet transplantation into kidney subcapsule and utilized it to determine the marginal islet mass sufficient for correction of hyperglycemia in diabetic nude mice.
authors.affiliation: Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea.
authors.forename: Jong-Yeon
authors.initials: JY
authors.lastname: Lim
authors.forename: Byoung-Hoon
authors.initials: BH
authors.lastname: Min
authors.forename: Byoung-Geun
authors.initials: BG
authors.lastname: Kim
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Han
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
authors.forename: Sung-Sik
authors.initials: SS
authors.lastname: Han
authors.forename: Jun-Seop
authors.initials: JS
authors.lastname: Shin
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000473:pathology / Q000601:surgery; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007668:Kidney / Q000473:pathology / Q000601:surgery*; D051379:Mice; D008819:Mice, Nude; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D015718:Fibrin Tissue Adhesive; D007328:Insulin