[The role of hepatic stellate cells SSeCKS expression in liver fibrosis].
OBJECTIVE
To investigate the change and effect of SSeCKS (src suppressed c kinase substrates) in the activation of hepatic stellate cells (HSCs).


METHODS
HSCs were isolated from normal rats, the change of SSeCKS mRNA expression on HSCs culture in vitro was determined using real-time PCR, protein level was determined by Western blot and immunofluorescence methods. A rat model of liver fibrosis was established. The expression and location of SSeCKS and alpha-SMA (alpha-smooth muscle actin) in liver tissues were detected by immunofluorescence methods.


RESULTS
SSeCKS mRNA expression was low in freshly isolated HSCs cell and the expression increased in activated HSCs in vitro. In liver fibrosis tissue, the number of SSeCKS-positive cells was increased and these cells were distributed along the sinusoids which also contained alpha-SMA positive cells.


CONCLUSIONS
The expression of SSeCKS was increased in activated HSCs in vitro. Therefore, SSeCKS may be involved in the liver inflammation and fibrosis.
Jiang
Wen
W
Department of Gastroenterology, Nantong Third People's Hospital, Nantong 226006, China. jwntsy@126.com
Song
Lei
L
Shao
Jian-Guo
JG
Wang
Lu-Jun
LJ
Lu
Jian-Rong
JR
Liu
Hai-Ou
HO
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism*; D011493:Protein Kinase C / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D012333:RNA, Messenger; D011493:Protein Kinase C