[Degradation of electrodeposited calcium phosphate and calcium phosphate-chitosan coatings in vitro].
OBJECTIVE
To investigate the degradation of electrodeposited calcium phosphate (ECP) coating and calcium phosphate/chitosan (ECPC) coating in vitro.


METHODS
Osteoclasts were isolated from neonatal rabbit long bone cavities and incubated with ECP and ECPC coatings. Calcium ion concentrations in the culture medium were analyzed at 3 days and 6 days. The osteoclastic resorption was observed with scanning electron microscope.


RESULTS
Both coatings demonstrated osteoclastic resorption lacunae. The calcium ion concentrations of the culture mediums were decreased when incubated with calcium phosphate coatings (P < 0.05). Compared with coatings cultured with osteoclasts, the calcium ion concentrations of those cultured without osteoclasts were higher on day 3 (P > 0.05) but lower on day 6 (P < 0.05).


CONCLUSIONS
Both ECP and ECPC coatings can be resorbed by osteoclasts in vitro and can dissolve in the culture medium.
Wang
Li-Na
LN
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Wang
Yi-Ning
YN
Wang
Jia-Wei
JW
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000378:metabolism*; D002478:Cells, Cultured; D048271:Chitosan / Q000008:administration & dosage / Q000378:metabolism*; D003722:Dental Alloys; D004563:Electrochemistry; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D011817:Rabbits
D002130:Calcium Phosphates; D003722:Dental Alloys; D048271:Chitosan; C020243:calcium phosphate; D002118:Calcium