Expression patterns of Nedd4 isoforms and SGK1 in the rat cochlea.
CONCLUSIONS
Our findings suggest that there exists a Na(+) transport system in the cochlea consisting of SGK1, Nedd4 isoforms and ENaC, which may work in concert to transport Na(+) and to maintain homeostasis in the inner ear as they do in other tight epithelia.


OBJECTIVE
It has been well demonstrated that the epithelial sodium channel (ENaC) plays important roles in regulation of Na(+) reabsorption in the tight epithelia of kidney, colon and lung. Recent studies have provided multiple evidence that the serum glucocorticoid-inducible kinase1(SGK1) and the ubiquitin-protein ligase neural precursor cell-expressed, developmentally downregulated isoforms Nedd4 are involved in ENaC regulation in response to hormones such as aldosterone, vasopressin and insulin. The authors aimed to investigate the cellular localization of Nedd4 isoforms, Nedd4-1/2 and Nedd4-2, and SGK1 in the rat cochlea.


METHODS
The expression patterns of Nedd4-1/2, Nedd4-2 and SGK1 in the rat cochlea were studied by immunohistochemistry with the specific polyclonal rabbit antibodies against the rat Nedd4-1/2, Nedd4-2 and SGK1.


RESULTS
All three proteins were extensively expressed in various regions of the rat cochlea. They were found in the stria vascularis, spiral ligament, organ of Corti, spiral limbus, spiral ganglion and Reissner's membrane.
Zhong
Shi-Xun
SX
Department of Otolaryngology, The First Affiliated Hospital Chongqing Medical University, No.1 Youyi Road, Yuzhong District, Chongqing, 400016, P.R. China.
Liu
Zhao-Hua
ZH
D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D005260:Female; D017874:Immediate-Early Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D000075702:Nedd4 Ubiquitin Protein Ligases; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D000099184:Serum-Glucocorticoid Regulated Kinases
D056827:Endosomal Sorting Complexes Required for Transport; D017874:Immediate-Early Proteins; D007527:Isoenzymes; D000075702:Nedd4 Ubiquitin Protein Ligases; D017346:Protein Serine-Threonine Kinases; D044767:Ubiquitin-Protein Ligases; D000099184:Serum-Glucocorticoid Regulated Kinases; C000618479:NEDD4L protein, rat; C000618484:Nedd4 protein, rat