[Transfection of pEGFP-C2 in brain mediated by targeting liposome P-MMA-DOSPER].
This research tried improving the specificity and efficiency of gene transfection in gene therapy and tried making the liposome a better gene transfer vector to brain by use of the monoclonal antibody (anti-Lex/SSEA-1)-mediated targeting of liposome. The derivatized monoclonal antibody was conjugated to the liposome DOSPER to form the targeting liposome P-MMA-DOSPER. Then, the pEGFP-C2 encapsulated in P-MMA-DOSPER or DOSPER was injected into the lateral ventricle of SD rats respectively, and the brains were taken for frosted slice 1, 3, 7 or 14 days later. The expression of GFP was observed under fluorescent microscope. There was a lot of expression of GFP around the lateral ventricle of rats in each group. But the indirect fluorescence antibody test showed the ratio of GFP+/nestin+ cells to nestin+ cells of every marking time point in the group of P-MMA-DOSPER was higher than the one in the group of DOSPER; the difference was found to be statistically significant (P<0.01). The results proved that the P-MMA-DOSPER can permeat the ependyma and can transfer gene into the nerve stem cells in vivo safely and effectively.
Tang
Shaonian
S
Department of Neurolog of Zhujiang Hospital Affiliated to the Southern Medical University, Guangzhou 510282, China.
Liu
Zhenhua
Z
Zhao
Lianxu
L
Zou
Zhihao
Z
Du
Mouxuan
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D008081:Liposomes; D008297:Male; D019904:Polymethyl Methacrylate / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
C118665:1,3-dioleoyloxy-2-(6-carboxyspermyl)propylamide; D000911:Antibodies, Monoclonal; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D049452:Green Fluorescent Proteins; D019904:Polymethyl Methacrylate