title: [Degradation of electrodeposited calcium phosphate and calcium phosphate-chitosan coatings in vitro].
abstract: OBJECTIVE
  To investigate the degradation of electrodeposited calcium phosphate (ECP) coating and calcium phosphate/chitosan (ECPC) coating in vitro.
  
  
  METHODS
  Osteoclasts were isolated from neonatal rabbit long bone cavities and incubated with ECP and ECPC coatings. Calcium ion concentrations in the culture medium were analyzed at 3 days and 6 days. The osteoclastic resorption was observed with scanning electron microscope.
  
  
  RESULTS
  Both coatings demonstrated osteoclastic resorption lacunae. The calcium ion concentrations of the culture mediums were decreased when incubated with calcium phosphate coatings (P < 0.05). Compared with coatings cultured with osteoclasts, the calcium ion concentrations of those cultured without osteoclasts were higher on day 3 (P > 0.05) but lower on day 6 (P < 0.05).
  
  
  CONCLUSIONS
  Both ECP and ECPC coatings can be resorbed by osteoclasts in vitro and can dissolve in the culture medium.
authors.affiliation: Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Wang
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Wang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000378:metabolism*; D002478:Cells, Cultured; D048271:Chitosan / Q000008:administration & dosage / Q000378:metabolism*; D003722:Dental Alloys; D004563:Electrochemistry; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D011817:Rabbits
chemical_list: D002130:Calcium Phosphates; D003722:Dental Alloys; D048271:Chitosan; C020243:calcium phosphate; D002118:Calcium