title: [Detection of HBV DNA among HBsAg negative children after prevention for mother-to-infant transmission].
abstract: OBJECTIVE
  Detection HBV DNA among HBsAg negative children who have been vaccined at birth, in order to improve the evaluation of the indicator for HBV DNA infection.
  
  
  METHODS
  Selection HBsAg negative children who have been vaccined at birth and then detection HBV DNA from sera using QIAamp Viral DNA Mini Kit, HBV DNA s region was obtained by nested PCR and sequencing.
  
  
  RESULTS
  12 of the 140 children were HBV DNA detected were positive and the infectious rate was 8.6% . No mutant of the 12 HBV DNA in "a" determinant.
  
  
  CONCLUSIONS
  To evaluate the effection of the prevention of HBV mother-to-child transmission, the standard method should be established. The detection of HBV DNA should be included in the future.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Bian
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Shen
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Yan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000517:prevention & control* / Q000635:transmission*; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control*; D008297:Male; D009154:Mutation
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens