title: Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins.
abstract: Bordetella pertussis is the causative agent of highly communicable respiratory infection whooping cough (pertussis) which remains one of the world's leading causes of vaccine-preventable deaths. In the present study, total soluble proteins extracted from two B. pertussis strains, Tohama I and the local isolate Saadet were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the antisera obtained from the mice immunized with inactivated whole cells as well as those collected from the mice challenged intraperitoneally with live cells of each strain. Of a total of 25 immunogenic proteins identified, 21 were shown to be the novel antigens for B. pertussis.
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey.
authors.forename: Emrah
authors.initials: E
authors.lastname: Altindiş
authors.forename: Burcu E
authors.initials: BE
authors.lastname: Tefon
authors.forename: Volkan
authors.initials: V
authors.lastname: Yildirim
authors.forename: Erkan
authors.initials: E
authors.lastname: Ozcengiz
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Gülay
authors.initials: G
authors.lastname: Ozcengiz
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D001886:Bordetella pertussis / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D021382:Protein Sorting Signals; D020543:Proteome; D040901:Proteomics / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D020543:Proteome