[Analysis of the genotype of intraspecies of common Mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences].
OBJECTIVE
To analyze the genotypes of intraspecies of common mycobacteria.


METHODS
The genotypes of 94 strains of mycobacteria from DSMZ, as compared with 12 reference strains, were studied by 16S-23S rRNA internal transcription space (ITS) sequence analysis.


RESULTS
The sequencing of 16S-23S rRNA ITS of the 106 strains of common mycobacteria were completed. All the mycobacteria could be discriminated to several genotypes except 4 M. intracellulare, 4 M. avium, 6 M. marinum and 2 M. malmoense which were identical to their reference strains.


CONCLUSIONS
16S-23S rRNA ITS sequence analysis is a reliable method to discriminate mycobacteria in interspecies even in intraspecies.
Yan
Li-Xi
LX
Lanzhou Medical College, Lanzhou University, Lanzhou 730000, China.
Ju
Jun
J
Chen
Bao-Wen
BW
Zhu
Bing-Dong
BD
Wang
Guo-Zhi
GZ
D021903:DNA, Ribosomal Spacer / Q000235:genetics; D020459:Genes, rRNA; D005838:Genotype; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014158:Transcription, Genetic
D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S