title: Interspliced transcription chimeras: neglected pathological mechanism infiltrating gene accession queries?
abstract: Over half of the DNA of mammalian genomes is transcribed, and one of the emerging enigmas in the field of RNA research is intergenic splicing or transcription induced chimerism. We argue that fused low-copy-number transcripts constitute neglected pathological mechanism akin to copy number variation, due to loss of stoichiometric subunit ratios in protein complexes. An obstacle for transcriptomics meta-analysis of published microarrays is the traditional nomenclature of merged transcript neighbors under same accession codes. Tandem transcripts cover 4-20% of genomes but are only loosely overlapping in population. They were most enriched in systems medicine annotations concerning neurology, thalassemia and genital disorders in the GeneGo Inc. MetaCore-MetaDrug(TM) knowledgebase, evaluated with external randomizations here. Clinical transcriptomics is good news since new disease etiologies offer new remedies. We identified homeotic HOX-transfactors centered around BMI-1, the Grb2 adaptor network, the kallikrein system, and thalassemia RNA surveillance as vulnerable hotspot chimeras. As a cure, RNA interference would require verification of chimerism from symptomatic tissue contra healthy control tissue from the same patient.
authors.affiliation: Institute of Medical Technology, University of Tampere, Finland.
authors.forename: Martti
authors.initials: M
authors.lastname: Tolvanen
authors.forename: Pauli J
authors.initials: PJ
authors.lastname: Ojala
authors.forename: Petri
authors.initials: P
authors.lastname: Törönen
authors.forename: Heidi
authors.initials: H
authors.lastname: Anderson
authors.forename: Jukka
authors.initials: J
authors.lastname: Partanen
authors.forename: Hannu
authors.initials: H
authors.lastname: Turpeinen
mesh_terms: D000465:Algorithms; D030561:Databases, Nucleic Acid; D018628:Gene Dosage; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks; D023281:Genomics / Q000379:methods*; D006801:Humans; D050506:Mutant Chimeric Proteins / Q000235:genetics*; D034622:RNA Interference; D012326:RNA Splicing
chemical_list: D050506:Mutant Chimeric Proteins