A high sensitive and specific QDs FRET bioprobe for MNase.
We used CdSe/ZnS quantum dots-ssDNA-fluorescent dye conjugates as bioprobes to detect micrococcal nuclease with high specificity and sensitivity, and further utilized the bioprobe to monitor the micrococcal nuclease activity in the culture medium of Staphylococcus aureus by fluorescence microscopy.
Huang
Shan
S
Research Center of Analytical Science, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, PR China.
Xiao
Qi
Q
He
Zhi Ke
ZK
Liu
Yi
Y
Tinnefeld
Philip
P
Su
Xiong Rui
XR
Peng
Xiao Niu
XN
D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008836:Micrococcal Nuclease / Q000032:analysis* / Q000378:metabolism; D036103:Nanotechnology; D045663:Quantum Dots; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013211:Staphylococcus aureus / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
D019187:Cadmium Compounds; D012235:Rhodamines; D017967:Zinc Compounds; D008836:Micrococcal Nuclease