A rapid molecular method for characterising bacterial bioburden in chronic wounds.
Polymerase chain reaction is a culture-free method of identifying pathogens. This case study describes how, unlike traditional culture reports, a modification of this technique rapidly determined that Pseudomonas spp. was present in a chronic wound.
Wolcott
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RD
Southwest Regional Wound Care Center, Lubbock, TX, USA.
Dowd
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SE
D000368:Aged; D015373:Bacterial Typing Techniques / Q000379:methods*; D018441:Biofilms / Q000254:growth & development; D002908:Chronic Disease; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007871:Leg Ulcer / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification; D017592:Skin Care; D013997:Time Factors; D014946:Wound Infection / Q000382:microbiology*
D004269:DNA, Bacterial