[Recombinant envelope glycoprotein domain III of dengue virus inhibit virus infection].
OBJECTIVE
To observe the ability of dengue virus recombinant envelope protein domain expressed in E. coli to inhibit virus infection and induce the neutralizing antibody.


METHODS
E III protein of Dengue virus serotypes 1-4 were expressed in E. coli BL21(DE3) then purified. Recombinant proteins were tested to inhibit DV2 from infecting BHK-21 cell. Rabbits were immunized with recombinant proteins to produce anti-E III serum. Antibody titers were determined by neutralizing assay.


RESULTS
The recombinant E III proteins of Dengue virus serotypes 1-4 were expressed in E. coli. They effectively protected BHK cells in culture against DV2 infection. All four type anti-E III sera can neutralize DV2 but their efficacies are different.


CONCLUSIONS
proteins of dengue virus expressed in E. coli can directly inhibit DV2 infection. Neutralizing antibodies were induced by E III proteins. Both E III protein of dengue virus and the neutralizing antibodies they induced are more efficient in inhibiting homologous dengue serotypes infection than heterologous serotypes.
Lu
Peng
P
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Wei
Yan
Y
Cao
Shou-Chun
SC
Li
Jian-Dong
JD
Liu
Qin-Zhi
QZ
Zhang
Quan-Fu
QF
Li
Chuan
C
Miao
Fang
F
Zhang
Shuo
S
Hang
Xiao-Tong
XT
Liang
Mi-Fang
MF
Li
De-Xin
DX
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015536:Down-Regulation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007114:Immunization; D008647:Mesocricetus; D017434:Protein Structure, Tertiary; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins