Proteins associated with immunopurified granules from a model pancreatic islet beta-cell system: proteomic snapshot of an endocrine secretory granule.
beta-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-1E beta-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and beta-cell death in type-2 diabetes.
Hickey
Anthony J R
AJ
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Bradley
Joshua W I
JW
Skea
Gretchen L
GL
Middleditch
Martin J
MJ
Buchanan
Christina M
CM
Phillips
Anthony R J
AR
Cooper
Garth J S
GJ
D000682:Amyloid / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000378:metabolism; D004703:Endocrine System / Q000378:metabolism*; D004798:Enzymes / Q000737:chemistry; D006728:Hormones / Q000378:metabolism; D006801:Humans; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D058228:Islet Amyloid Polypeptide; D013058:Mass Spectrometry / Q000379:methods; D018832:Molecular Chaperones / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D051381:Rats; D022142:Secretory Vesicles / Q000378:metabolism*
D000682:Amyloid; D004798:Enzymes; D006728:Hormones; D058228:Islet Amyloid Polypeptide; D018832:Molecular Chaperones; D020543:Proteome