Quantitative RT PCR approach to evaluate the neurogenic and gliagenic gene expression of cultured human amniotic epithelial cells.
The aim of the study is to determine the neuronal and glial gene expression of cultured human amniotic epithelial cells (HAECs) in serial passages. HAECs obtained from human term placentae were cultured in F12:DMEM (1:1) + 10% FBS +10ng/ml EGF in serial passages. Quantitative RT-PCR was used to assess the gene expression analysis. The results showed that the cultured HAECs expressed the neural stem cell genes (Nestin, NSE and Vimentin), mature neuronal genes (TH, MAP-2, beta-III-tubulin and NFM) and glial genes (CNPase, MBP and Olig). These neural stem cell genes increased with serial passages while the genes expression for mature neuronal and glial cells were downregulated. These results suggested that HAECs may promote or involve in neurogenesis and gliagenesis.
Tan
G C
GC
Department of Pathology, Universiti Kebangsaan Malaysia Medical Center.
Simat
S F
SF
Abdul Rahman
H
H
Tan
A E
AE
Chua
K H
KH
D000650:Amnion / Q000166:cytology*; D002478:Cells, Cultured; D004848:Epithelium; D015870:Gene Expression; D006801:Humans; D009457:Neuroglia / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D013234:Stem Cells