Immobilization of catalysts of biological interest on porous oxidized silicon surfaces.
The present paper deals with the immobilization of redox mediators and proteins onto protected porous silicon surfaces to obtain their direct electrochemical reactions and to retain their bioactivities. This paper shows that MP-11 and viologens are able to establish chemical bonds with 3-aminopropyltriethoxylsilane-modified porous silicon surface. The functionalization of the surfaces have been fully characterized by energy dispersive X-ray analysis (EDX) and X-ray photoelectron spectroscopy (XPS) to examine the immobilization of these mediators onto the solid surface. Amperometric and open circuit potential measurements have shown the direct electron transfer between glucose oxidase and the electrode in the presence of the viologen mediator covalently linked to the 3-aminopropyltriethoxylsilane (APTES)-modified porous silicon surfaces.
Alves
Wendel A
WA
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP, Brazil.
Fiorito
Pablo A
PA
Froyer
Gerard
G
El Haber
Fady
F
Vellutini
Luc
L
Torresi
Roberto M
RM
de Torresi
Susana I Córdoba
SI
D001234:Aspergillus niger / Q000378:metabolism; D004583:Electrons; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013997:Time Factors
D011437:Propylamines; D012821:Silanes; D005949:Glucose Oxidase; D005947:Glucose; C477625:amino-propyl-triethoxysilane; D010100:Oxygen; D012825:Silicon