title: Stability of screening compounds in wet DMSO.
abstract: The impact of storage conditions on compound stability and compound solubility has been debated intensely over the past 5 years. At Novartis, the authors decided to opt for a storage concept that can be considered controversial because they are using a DMSO/water (90/10) mixture as standard solvent. To assess the effect of water in DMSO stocks on compound stability, the authors monitored the purity of a subset of 1404 compounds from ongoing medicinal chemistry projects over several months. The study demonstrated that 85% of the compounds were stable in wet DMSO over a 2-year period at 4 degrees C. This result validates the storage concept developed at Novartis as a pragmatic approach that takes advantage of the benefits of DMSO/water mixtures while mediating the disadvantages. In addition, the authors describe how purity data collected over the course of the chemical validation of high-throughput screening actives are used to improve the analytical quality of the Novartis screening deck.
authors.affiliation: Novartis Institutes for BioMedical Research, Basel, Switzerland. Caroline.Engeloch@novartis.com
authors.forename: Caroline
authors.initials: C
authors.lastname: Engeloch
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schopfer
authors.forename: Ingo
authors.initials: I
authors.lastname: Muckenschnabel
authors.forename: Francois
authors.initials: F
authors.lastname: Le Goff
authors.forename: Hervé
authors.initials: H
authors.lastname: Mees
authors.forename: Karoline
authors.initials: K
authors.lastname: Boesch
authors.forename: Maxim
authors.initials: M
authors.lastname: Popov
mesh_terms: D002853:Chromatography, Liquid; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D004355:Drug Stability; D013058:Mass Spectrometry; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000302:isolation & purification; D012984:Software; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry*
chemical_list: D054852:Small Molecule Libraries; D014867:Water; D004121:Dimethyl Sulfoxide