Termination of equine atrial fibrillation by quinidine: an optical mapping study.
OBJECTIVE
To perform the first optical mapping studies of equine atrium to assess the spatiotemporal dynamics of atrial fibrillation (AF) and of its termination by quinidine.


METHODS
Intact, perfused atrial preparations obtained from four horses with normal cardiovascular examinations.


METHODS
AF was induced by a rapid pacing protocol with or without acetylcholine perfusion, and optical mapping was used to determine spatial dominant frequency distributions, electrical activity maps, and single-pixel optical signals. Following induction of AF, quinidine gluconate was perfused into the preparation and these parameters were monitored during quinidine-induced termination of AF.


RESULTS
Equine AF develops in the context of spatial gradients in action potential duration (APD) and diastolic interval (DI) that produce alternans, conduction block, and Wenckebach conduction in different regions at fast pacing rates. Quinidine terminates AF and prevents subsequent reinduction by reducing the maximal frequency and increasing frequency homogeneity.


CONCLUSIONS
Heterogeneity of APD and DI promote alternans and conduction block at fast pacing rates in the equine atrium, predisposing to the development of AF. Quinidine terminates AF by reducing maximum frequency and increasing frequency homogeneity. Our results are consistent with the hypothesis that quinidine increases effective refractory period, thereby decreasing frequency.
Fenton
Flavio H
FH
Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA.
Cherry
Elizabeth M
EM
Kornreich
Bruce G
BG
D000109:Acetylcholine / Q000494:pharmacology; D000200:Action Potentials; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000627:therapeutic use*; D001281:Atrial Fibrillation / Q000188:drug therapy / Q000662:veterinary*; D006325:Heart Atria / Q000187:drug effects*; D006734:Horse Diseases / Q000188:drug therapy*; D006736:Horses; D011802:Quinidine / Q000627:therapeutic use*; D046509:Tissue Culture Techniques / Q000662:veterinary
D000889:Anti-Arrhythmia Agents; D011802:Quinidine; D000109:Acetylcholine