Organic metabolites in exhaled human breath--a multivariate approach for identification of biomarkers in lung disorders.
The gas chromatographic profiles of exhaled air from lung cancer patients have been investigated. The breath from healthy volunteers, smokers and non-smokers, and lung cancer patients without treatment and under radio and/or chemotherapy, was collected using Tedlar bags. Different profiles for healthy people and cancer patients could be recognized by multivariate analysis and significant diagnostic compounds could be established. Target compounds showed to be linear and branched hydrocarbons between C(14) and C(24). Solid phase microextraction (SPME) coupled to gas chromatography mass spectrometry GC-(TOF)-MS was used. The method showed good precision (RSD below 26%) and limit of detection ranged from 0.04 to 8.0ppb. These findings show a high potential for establishment of laboratorial screening methods. Validation studies in a larger number of patients are being done.
Gaspar
Elvira M
EM
Chemistry Department, CQFB-Requimte, Faculty of Sciences and Technology, New University of Lisbon, Quinta da Torre, 2825-114 Monte da Caparica, Portugal.
Lucena
Ana F
AF
Duro da Costa
J
J
Chaves das Neves
H
H
D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D045853:Exhalation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006838:Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012907:Smoking / Q000378:metabolism; D052616:Solid Phase Extraction; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D015415:Biomarkers; D006838:Hydrocarbons; D055549:Volatile Organic Compounds