19F NMR spectroscopy for the analysis of RNA secondary structure populations.
Labeling of RNA with site-specific fluorine atoms has become straightforward in recent years and in particular has become an integrated part of engineered functional RNA with therapeutic potential, e.g. for siRNA technologies. Here, we demonstrate that temperature-dependent one-dimensional (19)F NMR spectroscopy of oligoribonucleotides is a powerful tool to obtain direct information on the conformational behavior. The approach is particularly useful for the quantification of monomolecular vs bimolecular secondary structure equilibria.
Graber
Dagmar
D
University of Innsbruck, Institute of Organic Chemistry, Innrain 52a, 6020 Innsbruck, Austria.
Moroder
Holger
H
Micura
Ronald
R
D005462:Fluorine Radioisotopes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry; D017433:Protein Structure, Secondary; D012313:RNA / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D014466:Ultraviolet Rays
D005462:Fluorine Radioisotopes; D009696:Nucleic Acids; D009843:Oligoribonucleotides; D034741:RNA, Small Interfering; D012313:RNA