title: The stemness gene expression of cultured human amniotic epithelial cells in serial passages.
abstract: The aim of the study is to evaluate the stemness gene expression of cultured human amniotic epithelial cells (HAECs) in serial passages. HAECs obtained from human term placentae were cultured in F12:DMEM(1:1) + 10% FBS +10ng/ml EGF in serial passages (P0, P1, P2 and P4). Quantitative RT-PCR was used to assess the gene expression analysis. The results showed that cultured HAECs expressed and downregulated the stemness genes expression for Oct-4, Sox-2, Nanog3, FGF4, Rex-1, FZD-9, BST-1 ABCG2. However, vimentin and nestin gene expression were upregulated. The results suggested that cultured HAECs may have pluripotent and multipotent properties.
authors.affiliation: Department of Pathology, Universiti Kebangsaan Malaysia.
authors.forename: S F
authors.initials: SF
authors.lastname: Simat
authors.forename: K H
authors.initials: KH
authors.lastname: Chua
authors.forename: H
authors.initials: H
authors.lastname: Abdul Rahman
authors.forename: A E
authors.initials: AE
authors.lastname: Tan
authors.forename: G C
authors.initials: GC
authors.lastname: Tan
mesh_terms: D000650:Amnion / Q000166:cytology*; D002478:Cells, Cultured; D004848:Epithelium / Q000637:transplantation*; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D016133:Polymerase Chain Reaction