title: Over-expression of nm23-H1 in HeLa cells provides cells with higher resistance to oxidative stress possibly due to raising intracellular p53 and GPX1.
abstract: OBJECTIVE
  To determine whether the antitumor factor nm23 is related with antioxidation.
  
  
  METHODS
  Full-length human nm23-H1 was cloned into a mammalianexpressing vector and transiently introduced into HeLa cells.
  
  
  RESULTS
  A remarkably low level of reactive oxygen species (ROS) was detected in the cells overexpressing nm23-H1. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays found that the cells transfected with a nm23- H1-expressing plasmid had higher viability and stronger resistance to oxidative stress. Immunoprecipitation tests revealed that endogenous nm23-H1 formed a protein complex with p53. Furthermore, the intracellular levels of p53 and p53- regulated gene GPX1 were obviously increased in the cells overexpressing nm23- H1. The downregulation of p53 in the cells overexpressing nm23-H1 resulted in a higher cellular ROS level and lower cell viability.
  
  
  CONCLUSIONS
  The findings suggest that nm23-H1 may act as a cellular protector against oxidative stress, possibly triggering the p53-related antioxidative pathway.
authors.affiliation: School of Medicine, Xi'an Jiao-Tong University, Xi'an 710061, China.
authors.forename: Run
authors.initials: R
authors.lastname: An
authors.forename: Yong-lie
authors.initials: YL
authors.lastname: Chu
authors.forename: Chan
authors.initials: C
authors.lastname: Tian
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Dai
authors.forename: Jing-hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D000094765:Glutathione Peroxidase GPX1
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; D016877:Oxidants; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; C517938:NME1 protein, human; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human