Using support vector machines to distinguish enzymes: approached by incorporating wavelet transform.
The enzymatic attributes of newly found protein sequences are usually determined either by biochemical analysis of eukaryotic and prokaryotic genomes or by microarray chips. These experimental methods are both time-consuming and costly. With the explosion of protein sequences registered in the databanks, it is highly desirable to develop an automated method to identify whether a given new sequence belongs to enzyme or non-enzyme. The discrete wavelet transform (DWT) and support vector machine (SVM) have been used in this study for distinguishing enzyme structures from non-enzymes. The networks have been trained and tested on two datasets of proteins with different wavelet basis functions, decomposition scales and hydrophobicity data types. Maximum accuracy has been obtained using SVM with a wavelet function of Bior2.4, a decomposition scale j=5, and Kyte-Doolittle hydrophobicity scales. The results obtained by the self-consistency test, jackknife test and independent dataset test are encouraging, which indicates that the proposed method can be employed as a useful assistant technique for distinguishing enzymes from non-enzymes.
Qiu
Jian-Ding
JD
Department of Chemistry, Nanchang University, Nanchang 330031, PR China. jdqiu@ncu.edu.cn
Luo
San-Hua
SH
Huang
Jian-Hua
JH
Liang
Ru-Ping
RP
D000818:Animals; D001185:Artificial Intelligence; D002627:Chemistry, Physical; D004798:Enzymes / Q000737:chemistry*; D005240:Feasibility Studies; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry
D004798:Enzymes; D011506:Proteins