The Ebola virus ribonucleoprotein complex: a novel VP30-L interaction identified.
The ribonucleoprotein (RNP) complex of Ebola virus (EBOV) is known to be a multiprotein/RNA structure, however, knowledge is rather limited regarding the actual protein-protein interactions involved in its formation. Here we show that singularly expressed VP35 and VP30 are present throughout the cytoplasm, while NP forms prominent cytoplasmic inclusions and L forms smaller perinuclear inclusions. We could demonstrate the existence of NP-VP35, NP-VP30 and VP35-L interactions, similar to those described for Marburg virus (MARV) based on the redistribution of protein partners into NP and L inclusion bodies. Significantly, a novel VP30-L interaction was also identified and found to form as part of an NP-VP30-L bridge structure, similar to that formed by VP35. The identification of these interactions allows a preliminary model of the EBOV RNP complex structure to be proposed, and may provide insight into filovirus transcriptional regulation.
Groseth
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A
National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg R3E 3R2, Canada. groseth@staff.uni-marburg.de
Charton
J E
JE
Sauerborn
M
M
Feldmann
F
F
Jones
S M
SM
Hoenen
T
T
Feldmann
H
H
D000818:Animals; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D020836:Protein Structure, Quaternary; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014709:Vero Cells; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014157:Transcription Factors; C479897:VP30 protein, ebola virus; D014758:Viral Core Proteins; D014764:Viral Proteins; C084818:nucleoprotein VP35, Ebola virus