SUMOylation of RORalpha potentiates transcriptional activation function.
SUMOylation regulates a variety of cellular processes, including control of transcriptional activities of nuclear receptors. Here, we present SUMOylation of orphan nuclear receptor, RORalpha by both SUMO-1 and SUMO-2. SUMOylation of RORalpha occurred on the 240th lysine residue at the hinge region of human protein. PIAS family members, PIASxalpha, PIAS3, and PIASy, increased SUMOylation of RORalpha, whereas SENP2 specifically removed SUMO from RORalpha. SUMOylation-defective mutant form of RORalpha exhibited decreased transcriptional activity on RORalpha-responsive promoters indicating that SUMOylation may positively regulate transcriptional function of RORalpha.
Hwang
Eun Ju
EJ
Department of Biological Sciences, Research Center for Women's Disease, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742, South Korea.
Lee
Ji Min
JM
Jeong
Jiyeong
J
Park
Joo Hyeon
JH
Yang
Young
Y
Lim
Jong-Seok
JS
Kim
Jung Hwa
JH
Baek
Sung Hee
SH
Kim
Keun Il
KI
D003546:Cysteine Endopeptidases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D011401:Promoter Regions, Genetic; D050828:Protein Inhibitors of Activated STAT / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D050828:Protein Inhibitors of Activated STAT; C539738:RORA protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C117909:SUMO2 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D015534:Trans-Activators; D003546:Cysteine Endopeptidases; C501833:SENP2 protein, human