A humanized anti-M2 scFv shows protective in vitro activity against influenza.
M2 is one of the most conserved influenza proteins, and has been widely prospected as a potential universal vaccine target, with protection predominantly mediated by antibodies. In this paper we describe the creation of a humanized single chain Fv from 14C2, a potent monoclonal antibody against M2. We show that the humanized scFv demonstrates similar activity to the parental mAb: it is able to recognize M2 in its native context on cell surfaces and is able to show protective in vitro activity against influenza, and so represents a potential lead antibody candidate for universal prophylactic or therapeutic intervention in influenza.
Gabbard
J
J
Department of Infectious Diseases, Animal Health Research Center, Influenza Pathogenesis and Immunology Research Center, University of Georgia, Athens, 30602-1563, USA.
Velappan
N
N
Di Niro
R
R
Schmidt
J
J
Jones
C A
CA
Tompkins
S M
SM
Bradbury
A R M
AR
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000914:Antibodies, Viral / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D002642:Chick Embryo; D004285:Dogs; D005434:Flow Cytometry; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D007251:Influenza, Human / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D014763:Viral Matrix Proteins / Q000276:immunology*; D010948:Viral Plaque Assay; D000086082:Viroporin Proteins
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins