Disruption of Nectin-like 1 cell adhesion molecule leads to delayed axonal myelination in the CNS.
Nectin-like 1 (Necl-1) is a neural-specific cell adhesion molecule that is expressed in both the CNS and PNS. Previous in vitro studies suggested that Necl-1 expression is essential for the axon-glial interaction and myelin sheath formation in the PNS. To investigate the in vivo role of Necl-1 in axonal myelination of the developing nervous system, we generated the Necl-1 mutant mice by replacing axons 2-5 with the LacZ reporter gene. Expression studies revealed that Necl-1 is exclusively expressed by neurons in the CNS. Disruption of Necl-1 resulted in developmental delay of axonal myelination in the optic nerve and spinal cord, suggesting that Necl-1 plays an important role in the initial axon-oligodendrocyte recognition and adhesion in CNS myelination.
Park
Jinsil
J
Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, Kentucky 40292, USA.
Liu
Ben
B
Chen
Tao
T
Li
Hong
H
Hu
Xuemei
X
Gao
Jing
J
Zhu
Ying
Y
Zhu
Qiang
Q
Qiang
Boqin
B
Yuan
Jiangang
J
Peng
Xiaozhong
X
Qiu
Mengsheng
M
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules; D002450:Cell Communication / Q000502:physiology; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018390:Gene Targeting / Q000379:methods; D017930:Genes, Reporter / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D055495:Neurogenesis / Q000235:genetics*; D009836:Oligodendroglia / Q000502:physiology; D009900:Optic Nerve / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013997:Time Factors; D018183:Transplantation Chimera
D015815:Cell Adhesion Molecules; C498600:Igsf4b protein, mouse; D007136:Immunoglobulins; D008565:Membrane Proteins; D019006:Neural Cell Adhesion Molecules