title: Recruitment of transplanted dermal multipotent stem cells to sites of injury in rats with combined radiation and wound injury by interaction of SDF-1 and CXCR4.
abstract: Systemic transplantation of dermal multipotent stem cells has been shown to accelerate both hematopoietic recovery and wound healing in rats with combined radiation and wound injury. In the present study, we explored the mechanisms governing the recruitment of dermal multipotent stem cells to the sites of injury in rats with combined injury. Male dermal multipotent stem cells were transplanted into female rats, and using quantitative real-time PCR for the sex-determining region of Y chromosome, it was found that the amounts of dermal multipotent stem cells in irradiated bone marrow and wounded skin were far greater than those in normal bone marrow and skin (P < 0.01). However, incubation of dermal multipotent stem cells with AMD3100 before transplantation, which specifically blocks binding of stromal cell-derived factor 1 (SDF-1) to its receptor CXCR4, diminished the recruitment of dermal multipotent stem cells to the irradiated bone marrow and wounded skin by 58 +/- 4% and 60 +/- 4%, respectively (P < 0.05). In addition, it was confirmed that the expression of SDF-1 in irradiated bone marrow and wounded skin was up-regulated compared to that in their normal counterparts, and in vitro analysis revealed that irradiated bone marrow and wounded skin extracts had a strong chemotactic effect on dermal multipotent stem cells but that the effect decreased significantly when dermal multipotent stem cells were preincubated with AMD3100 (P < 0.05). These data suggest that transplanted dermal multipotent stem cells were recruited more frequently to the irradiated bone marrow and wounded skin than normal bone marrow and skin and that the interactions of SDF-1 and CXCR4 played a crucial role in this process.
authors.affiliation: Division 2, Department of Traumatology, Daping Hospital, Third Military Medical University, ChongQing, 400042, China. zongzhaowen2004@sina.com
authors.forename: Zhao-Wen
authors.initials: ZW
authors.lastname: Zong
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Cheng
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Su
authors.forename: Xin-Ze
authors.initials: XZ
authors.lastname: Ran
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Ai
authors.forename: Shi-Wu
authors.initials: SW
authors.lastname: Dong
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D001596:Benzylamines; D001853:Bone Marrow / Q000473:pathology* / Q000528:radiation effects; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000037:antagonists & inhibitors / Q000502:physiology*; D002633:Chemotaxis; D000080027:Cyclams; D020405:Dermis / Q000166:cytology; D005260:Female; D006571:Heterocyclic Compounds / Q000494:pharmacology; D008297:Male; D039902:Multipotent Stem Cells / Q000502:physiology / Q000637:transplantation*; D011833:Radiation Injuries, Experimental / Q000150:complications / Q000473:pathology* / Q000628:therapy; D051381:Rats; D017208:Rats, Wistar; D019718:Receptors, CXCR4 / Q000502:physiology*; D012867:Skin / Q000293:injuries / Q000473:pathology*; D015854:Up-Regulation; D014945:Wound Healing
chemical_list: D001596:Benzylamines; D054377:Chemokine CXCL12; D000080027:Cyclams; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; C088327:plerixafor