title: Band-selective 1H-13C cross-polarization in fast magic angle spinning solid-state NMR spectroscopy.
abstract: A magic angle spinning (MAS) NMR technique to transfer polarization from protons to a specific set of the (13)C spins is introduced for the study of biomolecular samples in the solid-state. Ultrafast (>60 kHz) MAS and low irradiation rf fields are used to achieve band-selective Hartmann-Hahn cross-polarization (CP) between the whole proton bath and carbons whose resonances are close to the (13)C-transmitter offset. When compared to conventional, broadband (1)H-(13)C CP, the band-selective experiment can be established without any loss of sensitivity when polarizing the aliphatic signals of a protein sample, and with a significant gain when polarizing carbonyls. This scheme can be used as a building block in 2D (13)C-(13)C homonuclear correlation experiments to obtain a faster and more sensitive characterization of biological solids.
authors.affiliation: Université de Lyon, CNRS/ENS Lyon/UCB-Lyon 1, Centre RMN à Très Hauts Champs, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: Ségolène
authors.initials: S
authors.lastname: Laage
authors.forename: Alessandro
authors.initials: A
authors.lastname: Marchetti
authors.forename: Julien
authors.initials: J
authors.lastname: Sein
authors.forename: Roberta
authors.initials: R
authors.lastname: Pierattelli
authors.forename: Hans Juergen
authors.initials: HJ
authors.lastname: Sass
authors.forename: Stephan
authors.initials: S
authors.lastname: Grzesiek
authors.forename: Anne
authors.initials: A
authors.lastname: Lesage
authors.forename: Guido
authors.initials: G
authors.lastname: Pintacuda
authors.forename: Lyndon
authors.initials: L
authors.lastname: Emsley
mesh_terms: D002244:Carbon / Q000737:chemistry; D002247:Carbon Isotopes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D011522:Protons; D013696:Temperature
chemical_list: D002247:Carbon Isotopes; D011522:Protons; D002244:Carbon