Pollution induced nitrative stress and heat shock protein 70 overexpression in fish liver mitochondria.
Exposure to heavy metals and organic pollutants in natural water bodies can have detrimental effects on fish health. A combination of biochemical and energy studies were used to observe the changes in fish liver mitochondria in response to environmental pollutant induced nitrative stress in natural field conditions. The fish samples Mugil cephalus were collected from polluted (Ennore) and unpolluted (Kovalam) estuaries for a period of two years. The results revealed elevated nitrite (NO2-) and nitrate (NO3-) levels, increased nitric oxide (NO) synthesis and 3-nitrotyrosine expression, decreased respiratory chain enzyme activities and ATP/ADP ratio, reduced mitochondrial superoxide dismutase (MnSOD), glutathione peroxidase (Gpx) levels, diminished thiol status that leads to alterations in the mitochondrial function and elevated mitochondrial heat shock protein 70 (mtHSP70) expression (30%) to a significant extent in fish from the polluted estuary than in the unpolluted estuary. The overexpression of HSP70 under stress may aid mitochondrial survival by protecting against nitrative stress induced damage. The results also reveal the percentage increase in fish liver mitochondrial HSP70 in response to cumulative effect of environmental pollutants.
Padmini
E
E
Research Department of Biochemistry, Bharathi Women's College, Chennai-600108, Tamilnadu, India. dstpadmini@rediffmail.com
Vijaya Geetha
B
B
Usha Rani
M
M
D000244:Adenosine Diphosphate / Q000032:analysis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D017753:Ecosystem; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D023701:Smegmamorpha / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D018840:HSP70 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D019803:Glutathione Disulfide