title: Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae).
abstract: Two full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2, were cloned from strains of the rice stem borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in field populations. Results showed a strong correlation between frequencies of the mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis.
authors.affiliation: Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Jiang
authors.forename: Mingjing
authors.initials: M
authors.lastname: Qu
authors.forename: Ian
authors.initials: I
authors.lastname: Denholm
authors.forename: Jichao
authors.initials: J
authors.lastname: Fang
authors.forename: Weihua
authors.initials: W
authors.lastname: Jiang
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Han
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017344:Genes, Insect; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology*; D007915:Lepidoptera / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D063086:Organothiophosphates / Q000494:pharmacology*; D012275:Oryza / Q000469:parasitology*; D010935:Plant Diseases / Q000469:parasitology*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods; D020539:Sequence Analysis, Protein; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D063086:Organothiophosphates; D014230:Triazoles; C012584:triazophos; D000110:Acetylcholinesterase