title: A high sensitive and specific QDs FRET bioprobe for MNase.
abstract: We used CdSe/ZnS quantum dots-ssDNA-fluorescent dye conjugates as bioprobes to detect micrococcal nuclease with high specificity and sensitivity, and further utilized the bioprobe to monitor the micrococcal nuclease activity in the culture medium of Staphylococcus aureus by fluorescence microscopy.
authors.affiliation: Research Center of Analytical Science, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.forename: Zhi Ke
authors.initials: ZK
authors.lastname: He
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Philip
authors.initials: P
authors.lastname: Tinnefeld
authors.forename: Xiong Rui
authors.initials: XR
authors.lastname: Su
authors.forename: Xiao Niu
authors.initials: XN
authors.lastname: Peng
mesh_terms: D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008836:Micrococcal Nuclease / Q000032:analysis* / Q000378:metabolism; D036103:Nanotechnology; D045663:Quantum Dots; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013211:Staphylococcus aureus / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D012235:Rhodamines; D017967:Zinc Compounds; D008836:Micrococcal Nuclease