[Association between polycyclic aromatic hydrocarbons exposure levels and nucleoplasmic bridge and nuclear bud frequencies in coke-oven workers].
OBJECTIVE
To seek new effect biomarkers as to evaluating the chromosomal damage in peripheral blood lymphocytes in coke-oven workers who were exposed to polycyclic aromatic hydrocarbons (PAHs).


METHODS
One hundred and fifty-eight coke-oven workers and 69 controls were recruited in this study. Nucleoplasmic bridges and nuclear buds were counted as indicators of chromosomal damage in terms of cytokinesis-block micronucleus (CBMN) test. Occupational history, age, sex, smoking and alcohol using status of all subjects were collected by questionnaire.


RESULTS
Frequencies of nucleoplasmic bridge in coke-oven workers were (9.41 +/- 3.73)% per hundred, and the frequencies of nuclear buds were (7.13 +/- 4.01)% per hundred, which were significantly higher (P < 0.01) than those of controls (1.88 +/- 1.49)% per hundred and (2.20 +/- 1.73)% per hundred respectively. The dose-effect relationships between nucleoplasmic bridges or nuclear buds and PAHs exposure levels were identified. Compared with male coke-oven workers, female workers had less nucleoplasmic bridges or nuclear buds. No effects of age, smoking and alcohol using were found on nucleoplasmic bridges or nuclear buds among coke-oven workers.


CONCLUSIONS
Nucleoplasmic bridges and nuclear buds might be effect biomarkers in coke-oven workers.
Duan
Hua-wei
HW
National Institute of Occupational Health Poison Control, Chinese Center for Disease Control Prevention, Beijing 100050, China.
Leng
Shu-guang
SG
Pan
Zu-fei
ZF
Niu
Yong
Y
Bin
Ping
P
Dai
Yu-fei
YF
Zheng
Yu-xin
YX
D000328:Adult; D000428:Alcohol Drinking; D002467:Cell Nucleus; D003077:Coke; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes; D008297:Male; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D012907:Smoking
D003077:Coke; D011084:Polycyclic Aromatic Hydrocarbons