title: The effect of human saliva substitutes in an erosion-abrasion cycling model.
abstract: Saliva may affect dental erosion-abrasion by reducing demineralization, enhancing remineralization, and acting as lubricant. This study tested the effect of human saliva substitutes in an erosion-abrasion cycling model designed for enamel and root dentin. Specimens were randomly assigned into the following groups (n = 8): artificial saliva (AS), artificial saliva + mucin (AS+M), deionized water (DIW, negative control), and pooled human saliva (HS, clinical reference). Each group was submitted to a cycle of 5 min in 1% citric acid (pH 3.75), 30 min in the testing solutions, and toothbrushing (enamel, 500 strokes; dentin, 150 strokes, approximately 200 g load) in fluoridated dentifrice (1,100 p.p.m. NaF) slurry. Specimens were rinsed and dried after each procedure. This cycle was repeated three times each day, for 3 d. Substrate loss was measured daily using optical profilometry. Analysis of variance (anova) and Tukey tests (alpha=0.05) showed a significant increase in enamel and dentin wear throughout the experiment for all groups. At the end of the experiment, enamel wear for each group was ranked as: (AS)<(AS+M) and (HS)<(DIW), with AS+M not differing from HS. For dentin, groups AS and AS+M did not differ from each other or from DIW, but showed significantly higher wear than HS. The artificial saliva with mucin showed promise as a potential substitute for human saliva in the enamel erosion-abrasion cycling model. For dentin, none of the artificial salivas performed similarly to human saliva.
authors.affiliation: Oral Health Research Institute, Department of Preventive and Community Dentistry, Indiana University School of Dentistry, Indianapolis, IN 46202-2876, USA. ahara@iupui.edu
authors.forename: Anderson T
authors.initials: AT
authors.lastname: Hara
authors.forename: Carlos
authors.initials: C
authors.lastname: González-Cabezas
authors.forename: Jonathan
authors.initials: J
authors.lastname: Creeth
authors.forename: Domenick T
authors.initials: DT
authors.lastname: Zero
mesh_terms: D003743:Dental Enamel / Q000187:drug effects*; D003799:Dental Stress Analysis / Q000379:methods; D003804:Dentin / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D008963:Molar; D011897:Random Allocation; D012307:Risk Factors; D012464:Saliva, Artificial / Q000494:pharmacology*; D014072:Tooth Abrasion / Q000150:complications*; D017001:Tooth Demineralization / Q000139:chemically induced / Q000209:etiology; D014077:Tooth Erosion / Q000150:complications*; D014089:Tooth Remineralization; D014099:Toothbrushing / Q000009:adverse effects
chemical_list: D012464:Saliva, Artificial