A large genome center's improvements to the Illumina sequencing system.
The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.
Quail
Michael A
MA
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK.
Kozarewa
Iwanka
I
Smith
Frances
F
Scally
Aylwyn
A
Stephens
Philip J
PJ
Durbin
Richard
R
Swerdlow
Harold
H
Turner
Daniel J
DJ
D000047:Academies and Institutes; D002874:Chromosome Mapping / Q000295:instrumentation*; D004867:Equipment Design; D023281:Genomics / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*