Interleukin-18 protein level is upregulated in adipose tissue of obese mice.
In this study, we investigated the regulation of Interleukin-18 (IL-18) and caspase-1 mRNA and protein levels in adipose and liver tissue of obese (ob/ob) mice compared with ob/+ mice. In ob/ob mice, which have a twofold higher IL-18 plasma level as compared with lean mice, IL-18 mRNA expression was significantly reduced by 1.6-fold in adipose tissue, whereas protein level was enhanced fourfold as compared with ob/+ mice. However, caspase-1 mRNA expression and activity were significantly enhanced in adipose tissue of ob/ob mice. Conversely, both IL-18 mRNA and protein levels were slightly enhanced, but caspase-1 activity was reduced in liver of ob/ob mice as compared with lean mice. In conclusion, we show that adipose and hepatic IL-18 protein expressions are increased in obese mice. However, in contrast to liver, the adipose IL-18 protein level appears to be upregulated through a post-transcriptional mechanism probably involving caspase-1.
Membrez
Mathieu
M
Department of Nutrition and Health, Nestlé Research Center, Lausanne, Switzerland.
Ammon-Zufferey
Corinne
C
Philippe
David
D
Aprikian
Olivier
O
Monnard
Irina
I
Macé
Katherine
K
Darimont
Christian
C
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D020170:Caspase 1 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
D020382:Interleukin-18; D012333:RNA, Messenger; D020170:Caspase 1