[Establishment of serum protein pattern model for screening pancreatic cancers by SELDI-TOF-MS technique].
OBJECTIVE
To detect the serum specific proteins in pancreatic cancer patients and establish diagnostic model by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) technique.


METHODS
Twenty-nine serum samples from patients of pancreatic cancer were collected before surgery and an additional 57 serum samples from age and sex matched individuals without cancer were used as controls, SELDI-TOF-MS technique and WCX magnetic beads were used to detect the protein fingerprint expression of all the serum samples and the resulting profiles between pancreatic cancer patients and controls were analyzed with biomarker wizard system, established the model using biomarker patterns system software. A double-blind test was used to determine the sensitivity and specificity of the classification model.


RESULTS
A panel of four biomarkers (relative molecular weight are 5705, 4935, 5318 and 3243 Da) were selected to set up a decision trees as the classification model for screening pancreatic cancer effectively. The result yielded a sensitivity of 100%, specificity of 97.4%. The double-blind test challenged the model with a sensitivity of 88.9% and a specificity of 89.5%.


CONCLUSIONS
SELDI-TOF-MS offers a unique platform for the proteomic detection of serum in pancreatic cancer patients. It also offers a noninvasive method to further study the proteomic changes in the development and progression of pancreatic cancer.
Ma
Ning
N
Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Ge
Chun-lin
CL
Luan
Feng-ming
FM
Hu
Chao-jun
CJ
Li
Yong-zhe
YZ
Liu
Young-feng
YF
D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D055088:Early Detection of Cancer; D006801:Humans; D008403:Mass Screening; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D001798:Blood Proteins