title: Studies of the toxicological potential of capsinoids: V. Genotoxicity studies of dihydrocapsiate.
abstract: A series of studies was performed to evaluate the safety of dihydrocapsiate (4-hydroxy-3-methoxybenzyl 8-methylnonanoate; CAS no. 205687-03-2). This study evaluated the potential genotoxicity of this compound using a variety of in vitro and in vivo test systems, including bacterial reverse mutation test, chromosomal aberration test, micronucleus test, gene mutation assay with transgenic rats, and single-cell gel (SCG) assay (Comet assay). In vitro tests (bacterial reverse mutation test and chromosomal aberration test) produced positive results in the absence of metabolic activation, but negative results in the presence of metabolic activation. The in vivo gene mutation assay (with transgenic rats) produced negative results, as did the in vivo mouse micronucleus assay, which failed to induce micronucleated polychromatic erythrocytes. Although the rat SCG assay produced statistically significant increases in the Olive tail moment and % tail DNA of the liver and intestine in the 2000 mg/kg group (compared with the negative-control group), a number of factors caused the authors to question the validity of these findings. Taken together, these results suggest that dihydrocapsiate has a low or extremely low likelihood of inducing genotoxicity.
authors.affiliation: SRA International, Inc., Cambridge, Maryland 21613, USA. Bernard@sra-intl.com
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Bernard
authors.forename: Eri
authors.initials: E
authors.lastname: Watanabe
authors.forename: Terutaka
authors.initials: T
authors.lastname: Kodama
authors.forename: Shoji
authors.initials: S
authors.lastname: Tsubuku
authors.forename: Akira
authors.initials: A
authors.lastname: Otabe
authors.forename: Madoka
authors.initials: M
authors.lastname: Nakajima
authors.forename: Shoji
authors.initials: S
authors.lastname: Masumori
authors.forename: Sawako
authors.initials: S
authors.lastname: Shimada
authors.forename: Jin
authors.initials: J
authors.lastname: Tanaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Masuyama
mesh_terms: D000818:Animals; D001711:Biotransformation; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000633:toxicity; D002869:Chromosome Aberrations; D020552:Comet Assay; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic
chemical_list: C470213:capsiate; D002211:Capsaicin