title: First isolation of Neospora caninum from the faeces of a dog from Portugal.
abstract: We report the in vitro isolation of Neospora caninum from the faeces of a naturally infected 8-year-old male stray boxer from Portugal. Vero cell cultures were infected using parasite stages obtained after oral inoculation of gamma-interferon knockout mice with 10(2) sporulated oocysts. The isolate was identified by microscopical examination, as well as histological, immunological and molecular methods including a DNA-microsatellite-based typing technique, and was subsequently named NC-P1. The DNA-microsatellite pattern observed in the NC-P1 isolate was not previously reported for any N. caninum isolate. To our knowledge, this is the first isolation of N. caninum from the faeces of a naturally infected dog from Portugal.
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany. wbasso@fcv.unlp.edu.ar
authors.forename: W
authors.initials: W
authors.lastname: Basso
authors.forename: D C
authors.initials: DC
authors.lastname: Herrmann
authors.forename: F J
authors.initials: FJ
authors.lastname: Conraths
authors.forename: N
authors.initials: N
authors.lastname: Pantchev
authors.forename: M Globokar
authors.initials: MG
authors.lastname: Vrhovec
authors.forename: G
authors.initials: G
authors.lastname: Schares
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology*; D008297:Male; D018688:Neospora; D011174:Portugal / Q000453:epidemiology