[Study on the selective removal of plasma low-density lipoprotein and fibrinogen by degraded guar sulfate].
Degraded guar was prepared by acid with guar as the main material, which was then brought into reaction with chlorosulfonic acid under proper conditions, the sulfonated degraded guar was obtained successfully. The effects of sulfonation conditions on the SO4(2-) content were investigated, and the proper reaction conditions were determined. The results of infrared spectrometry showed that this sulfated derivative is a novel heparin-like polysaccharide. At the same time, the selective removal of low density lipoprotein (LDL) and fibrinogen (Fib) by degraded guar gum sulfate was studied. The experimental results showed that degraded guar gum sulfate is a novel LDL/ Fib purifying agent. When pH= 5.15 and the initial concentration of the degraded guar gum sulfate is 2500 mg/L, the reduction percentages were about 60%-66% for total cholesterol, about 76%-89% for LDL and very low-density lipoproteins (VLDL), and almost 100% for fibrinogen. There were no significant changes regarding the level of high-density lipoproteins and total proteins.
Zhu
Ye
Y
Chemical Engineering Research Center, ECUST, Shanghai 200237, China.
Fang
Bo
B
Huang
Li
L
Guan
Chen
C
Yang
Guang
G
D005340:Fibrinogen / Q000032:analysis / Q000302:isolation & purification*; D005685:Galactans / Q000737:chemistry*; D006949:Hyperlipidemias / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D008351:Mannans / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
D005685:Galactans; D008077:Lipoproteins, LDL; D008351:Mannans; D053149:Plant Gums; D013431:Sulfates; D005340:Fibrinogen; C007894:guar gum