Identification of ICE2, a gene involved in cold acclimation which determines freezing tolerance in Arabidopsis thaliana.
Several transcription factors are presently known to regulate the response to cold stress. Here we describe a new positive regulator, ICE2, which is a transcription factor of the bHLH family that participates in the response to deep freezing through the cold acclimation-dependent pathway in Arabidopsis thaliana plants. An overexpression of ICE2 (as we named the At1g12860 locus) in transgenic Arabidopsis plants results in increased tolerance to deep freezing stress after cold acclimation. The seeds of transgenic lines that overexpressed ICE2 were characterized by decreased levels of carbohydrate and increased levels of lipids. The analysis of expression of CBF1 gene (also known as DREB1B), which have been shown to be required for the complete development of cold acclimation response in Arabidopsis indicated a difference between expression of the CBF1 gene in transgenic plants and the wild-type control plants, Col-0. These results suggested that the CBF1 transcription factor, known as one of the regulators of the cold stress response, has a dominant role in providing freezing tolerance in transgenic plants characterized by overexpression of ICE2.
Fursova
Oksana V
OV
N.I. Vavilov Institute of General Genetics Russian Academy of Sciences, Gubkin Str., 3, Moscow 119991 GSP-1, Russian Federation.
Pogorelko
Gennady V
GV
Tarasov
Valentin A
VA
D000064:Acclimatization / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D044367:Phase Transition; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D016415:Sequence Alignment; D016019:Survival Analysis; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D002241:Carbohydrates; D008055:Lipids; D012333:RNA, Messenger; D015534:Trans-Activators; C104387:CBF1 protein, Arabidopsis; C538823:ICE2 protein, Arabidopsis