Adenosine deaminase in patients with primary immunodeficiency syndromes: the analysis of serum ADA1 and ADA2 activities.
OBJECTIVE
We aimed to investigate the activity of ADA and its isoenzymes in serum of patients with various primary immunodeficiency (PID) syndromes.


METHODS
Total ADA (tADA) and its isoenzymes were measured in 76 children with PID syndromes and 30 healthy controls using the Ellis method.


RESULTS
Our results indicated that tADA and ADA2 levels were higher in patients with Chronic Granulomatous Disease (CGD), Leukocyte Adhesion Deficiency (LAD), hyper IgM (HIM) and Wiskott-Aldrich Syndrome (WAS) than those of corresponding controls (P<0.01). There was a significant elevation of tADA and ADA1 activities in IgA deficiency patients as compared to healthy individuals (P<0.01).


CONCLUSIONS
Our results hypothesized that altered ADA activity may be associated with altered immunity. Therefore, serum ADA level could be used as an indicator along with other parameters in follow up of patients with CGD, LAD, IgA deficiency, HIM and WAS.
Poursharifi
Pegah
P
Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran.
Saghiri
Reza
R
Ebrahimi-Rad
Mina
M
Nazem
Habibollah
H
Pourpak
Zahra
Z
Moin
Mostafa
M
Shams
Sedigheh
S
D000243:Adenosine Deaminase / Q000097:blood* / Q000378:metabolism; D000293:Adolescent; D000704:Analysis of Variance; D002648:Child; D002675:Child, Preschool; D006105:Granulomatous Disease, Chronic / Q000201:enzymology; D006801:Humans; D053306:Hyper-IgM Immunodeficiency Syndrome / Q000201:enzymology; D017098:IgA Deficiency / Q000201:enzymology; D017099:IgG Deficiency / Q000201:enzymology; D007223:Infant; D007492:Iran; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000201:enzymology; D016511:Severe Combined Immunodeficiency / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; D000243:Adenosine Deaminase