title: Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens.
abstract: Clinical reports have suggested the existence of immunological tolerance to noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched allogeneic stem cell transplantation (allo-SCT). We studied the T-cell reactivity using IFN-gamma enzyme-linked immunospot (ELISPOT) assay in three HLA fully matched allo-SCT cases and one healthy volunteer family case. In HLA fully matched allo-SCT cases, ELISPOT assay could detect the hyporesponsiveness of T cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed that the T cells from an individual responded to B cell from his mother significantly weakly than those from an unrelated HLA-haploidentical individual. These observations suggest that our IFN-gamma ELISPOT assay-based method may predict the presence of immunological tolerance to NIMA.
authors.affiliation: Division of Hematology and Oncology, Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: C
authors.initials: C
authors.lastname: Shimazaki
authors.forename: N
authors.initials: N
authors.lastname: Ochiai
authors.forename: E
authors.initials: E
authors.lastname: Maruya
authors.forename: Y
authors.initials: Y
authors.lastname: Akatsuka
authors.forename: E
authors.initials: E
authors.lastname: Ashihara
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
authors.forename: M
authors.initials: M
authors.lastname: Taniwaki
authors.forename: H
authors.initials: H
authors.lastname: Saji
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology*; D007371:Interferon-gamma; D008297:Male; D008431:Maternal-Fetal Exchange / Q000276:immunology; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D006680:HLA Antigens; D007371:Interferon-gamma