[RNA interference of hnRNP B1 gene in human A549 lung cells].
OBJECTIVE
To test the effect of RNA interference of hnRNP Bx gene in human lung cell line A549.


METHODS
The RNAs of cell line A549 were interfered by the RNA interference technique. The hnRNP B1 mRNAs were detected by fluorescence quantity RT-PCR. The proteins of hnRNP B1 were detected by Western blot technique. The growth rate of the cells was analysed by MTT.


RESULTS
The growth of the cells was suppressed significantly. The mRNA and protein expressions of hnRNP B1 were inhibited.


CONCLUSIONS
The expression of hnRNP B1 can be inhibited by RNA interference. RNA interference could become a possible clinical therapy for lung cancers.
Pu
Dan
D
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
Zhou
Tao-you
TY
Li
Wei-min
WM
Wu
Di
D
Chen
Xiao-bing
XB
Chen
Min
M
Tang
Feng-ming
FM
Han
Juan
J
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C439768:hnRNP A2