Electrical pulsed stimulation of surfaces homogeneously coated with gold nanoparticles to induce neurite outgrowth of PC12 cells.
In this study, the potential of gold nanoparticles (20 nm) to deliver electrical stimulation to nerve cell cultures in vitro to induce nerve regeneration was evaluated. In order to use these biomaterials to deliver an electrical stimulus, we devised a novel method for the fabrication of a nanostructured 2D substrate comprising gold nanoparticles attached to the surface of a cover glass via an adsorption system. In this strategy, gold nanoparticles are created and then coated onto a positively charged cover glass that has been pretreated with polyethyleneimine (PEI). Scanning electron microscopy (SEM) revealed that the PC 12 cells extended neurites well on the gold nanoparticles in the presence of electrical stimulation. In addition, the neurite outgrowth of PC12 cells in response to pulsed and constant electrical stimulation was evaluated by live/dead cell determination, by reverse transcriptase polymerase chain reaction (RT-PCR) analysis, as well as by beta-tubulin and NF-200 expression. By electrical stimulation (250 mV for 1 h), PC12 cells with their neurite outgrowth length were highly increased, with a mean diameter of 98.5 microm. However, the neurite outgrowth length without electrical stimulation was approximately 10 approximately 20 microm. Moreover, the alternating current stimulation also showed good viability (<90%), while a high amount of cell death (more than 30%) was observed with constant current stimulation. Thus, the gold nanoparticles with pulsed current stimulation may provide suitable tools for the nerve regeneration using neuronal cells.
Park
Ji Sun
JS
College of Medicine, Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
Park
Kyeongsoon
K
Moon
Hyun Tae
HT
Woo
Dae Gyun
DG
Yang
Han Na
HN
Park
Keun-Hong
KH
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004558:Electric Stimulation; D006046:Gold; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D016501:Neurites; D016716:PC12 Cells; D011094:Polyethyleneimine / Q000737:chemistry; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014404:Tubulin / Q000737:chemistry
D017931:DNA Primers; D014404:Tubulin; D006046:Gold; D011094:Polyethyleneimine