title: [Ferumoxide labeled Flk1+ CD31- CD34- human bone marrow mesenchymal stem cells and its in vivo tracing in the brains of Macaca Fascicularis].
abstract: OBJECTIVE
  To explore the method for labeling Flk1+ CD31- CD34- human bone marrow mesenchymal stem cells (hBMSCs) with ferumoxide-PLL and evaluate the feasibility of its tracing after transplantation into the brains of Macaca Fascicularis.
  
  
  METHODS
  The hBMSCs were incubated with ferumoxide-PLL. Trypan blue staining, Prussian blue staining, and transmission electron microscope were performed to show intracellular iron, marking efficiency, and the vigor of the labeled cells. After the hBMSCs were transplanted into the brains of cynomolgus monkeys by stereotaxis, magnetic resonance imaging (MRI) was performed to trace the cells in vivo. Cell survival and differentiation were studied with immunohistochemistry, Prussian blue staining, and HE staining.
  
  
  RESULTS
  The marking efficiency of the ferumoxide-PLL was 96%. Iron particles were found intracytoplasmic of the hBMSCs by Prussian blue staining and transmission electron microscopy. The relaxation rates of labeled cells in MRI were 4.4 and 4.2 times higher than those of the unlabeled cells. Hypointensity area was found by MRI three weeks after transplantation. Many hBMSCs and new vessels were found in the transplantation zone by pathological and immunofluorescence methods.
  
  
  CONCLUSIONS
  Ferumoxide-PLL can effectively label hBMSCs and thus increase its contrast in MRI results. The cells can survive in the brains of cynomolgus monkeys. The labeled hBMSCs can be traced in vivo by MRI.
authors.affiliation: Department of Neurosurgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Feng
authors.forename: Ren-Zhi
authors.initials: RZ
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Wang
authors.forename: Jun-Ji
authors.initials: JJ
authors.lastname: Wei
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yin
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Ma
authors.forename: Chuang
authors.initials: C
authors.lastname: Qin
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Zhao
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: An
authors.forename: Yan-Guo
authors.initials: YG
authors.lastname: Kong
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis / Q000378:metabolism; D001854:Bone Marrow Cells / Q000737:chemistry* / Q000378:metabolism; D016026:Bone Marrow Transplantation; D001921:Brain / Q000098:blood supply / Q000378:metabolism; D001923:Brain Chemistry; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D008252:Macaca fascicularis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000032:analysis / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D040301:Vascular Endothelial Growth Factor Receptor-2; C065507:ferumoxides; D052203:Ferrosoferric Oxide