title: Short-chain inulin-like fructans reduce endotoxin and bacterial translocations and attenuate development of TNBS-induced colitis in rats.
abstract: Anti-inflammatory effects of short-chain inulin-like fructans (SCF) on trinitrobenzene sulfonic acid (TNBS)-induced colitis were investigated in rats, focusing specifically on endotoxin and bacterial translocations. SCF with degrees of polymerization (DP) of 4 and 8 were used. Rats were fed either control diet or diets including 60 g DP4 or DP8 per kilogram for 7 days, and then received intracolonic TNBS and were fed the respective diets for a further 10 days. DP4 and DP8 significantly reduced colonic injuries as assessed by damage score, but the reduction of colonic myeloperoxidase activity was manifest solely with DP8. At 3 days after colitis induction, bacterial translocation to the mesenteric lymph node was significantly lower in the DP4 and DP8 groups, but significant reduction in the portal endotoxin concentration was achieved solely in the DP8 group. Immediately prior to colitis induction, cecal immunoglobulin A and mucin concentrations were higher in the DP4 and DP8 groups, but these changes were abolished at 10 days post colitis induction. The data suggest that SCF exert prophylactic effects against TNBS colitis, presumably as a result of inhibitory effects on endotoxin and bacterial translocations.
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tanabe
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
authors.forename: Wada
authors.initials: W
authors.lastname: Tadashi
authors.forename: Tomono
authors.initials: T
authors.lastname: Yasuhiko
authors.forename: Kimio
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Shuhachi
authors.initials: S
authors.lastname: Kiriyama
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Morita
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D002432:Cecum / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000382:microbiology* / Q000517:prevention & control; D004731:Endotoxins / Q000378:metabolism*; D005285:Fermentation; D005630:Fructans / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007070:Immunoglobulin A / Q000378:metabolism; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008643:Mesentery; D009077:Mucins / Q000378:metabolism; D011108:Polymers; D051381:Rats; D011916:Rats, Inbred F344; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D004731:Endotoxins; D005630:Fructans; D007070:Immunoglobulin A; D009077:Mucins; D011108:Polymers; D014302:Trinitrobenzenesulfonic Acid