[Comparison of transduction efficiencies of various gene vectors in human bone-marrow-derived mesenchymal stem cells].
OBJECTIVE
To compare the transduction efficiencies of adenoviral vector, adeno-associated viral vector, baculoviral vector, and plasmid vector in human bone-marrow-derived mesenchymal stem cells (hBMSCs).


METHODS
The hBMSCs were cultured in vitro and transducted with the adenoviral vector, adeno-associated viral vector, baculoviral vector, and plasmid vector. The expression of target protein was observed by inverted fluorescent microscopy and flow cytometry.


RESULTS
Inverted fluorescent microscopy showed that some of the hBMSCs after transduction expressed the green fluorescent protein (GFP) and the hBMSCs transducted with baculoviral vector expressed more GFP than those of other three vectors. Flow cytometry showed that the transduction efficiencies and mean fluorescence intensities of the adenoviral vector, adeno-associated viral vector, and plasmid vector were 42%, 37%, and 22% and 158, 115, and 77, respectively, which were significantly lower than those of baculoviral vector (70%, P < 0.01; 212, P < 0.05; respectively).


CONCLUSIONS
Compared with the adenoviral vector, adeno-associated viral vector, and plasmid vector, the baculoviral vector has higher transduction efficiency in hBMSCs and therefore may be a more suitable gene vector for research in human gene therapy.
Liu
Zheng-Shan
ZS
Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Zhang
Cheng
C
Shang
Yan-Chang
YC
Xiong
Fu
F
Feng
Shan-Wei
SW
Li
Yong
Y
Xu
Yong-Feng
YF
Zhou
Chang
C
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000821:virology*; D002478:Cells, Cultured; D000229:Dependovirus / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000821:virology*; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods*
D049452:Green Fluorescent Proteins