Cloning and expression pattern of a putative octopamine/tyramine receptor in antennae of the noctuid moth Mamestra brassicae.
In insects, biogenic amines have been shown to play an important role in olfactory plasticity. In a first attempt to decipher the underlying molecular mechanisms, we report the molecular cloning and precise expression pattern of a newly identified octopamine/tyramine-receptor-encoding gene in the antennae of the noctuid moth Mamestra brassicae (MbraOAR/TAR). A full-length cDNA has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction; the deduced protein exhibits high identities with previously identified octopamine/tyramine receptors in other moths. In situ hybridization within the antennae has revealed that MbraOAR/TAR is expressed at the bases of both pheromone-sensitive and non-sensitive olfactory sensilla and in cells with a neurone-like shape. In accordance with previous physiological studies that have revealed a role of biogenic amines in the electrical activity of the receptor neurones, our results suggest that biogenic amines (either octopamine or tyramine) target olfactory receptor neurones to modulate olfactory coding as early as the antennal level.
Brigaud
Isabelle
I
INRA, UMR 1272 INRA-UPMC-AgroParisTech PISC Physiologie de l'Insecte: Signalisation et Communication, Route de Saint-Cyr, 78000 Versailles, France.
Grosmaître
Xavier
X
François
Marie-Christine
MC
Jacquin-Joly
Emmanuelle
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism; D010802:Phylogeny; D017982:Receptors, Biogenic Amine / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012903:Smell / Q000502:physiology
D010675:Pheromones; D017982:Receptors, Biogenic Amine; C027604:norsynephrine receptor; C066175:tyramine receptor