title: A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping.
abstract: In a haematology ward, Candida parapsilosis was found in blood cultures from 4 patients within a month. As C. parapsilosis is known to have a restricted genetic diversity, a combined methodological approach was adopted to establish a possible epidemiological relationship among the isolates (n = 9). Multilocus sequence typing and random amplified polymorphic DNA analysis suggested a clonal origin of the isolates. The clonal origin was confirmed by microsatellite analysis, a method that displayed the highest discriminatory level and readily differentiated cluster isolates from 2 epidemiologically unrelated strains of C. parapsilosis. The use of novel methods of genotyping such as microsatellite analysis will facilitate epidemiological investigations of potential clonal outbreaks of fungaemia.
authors.affiliation: Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark.
authors.forename: Anna
authors.initials: A
authors.lastname: Brillowska-Dabrowska
authors.forename: T
authors.initials: T
authors.lastname: Schön
authors.forename: S
authors.initials: S
authors.lastname: Pannanusorn
authors.forename: N
authors.initials: N
authors.lastname: Lönnbro
authors.forename: L
authors.initials: L
authors.lastname: Bernhoff
authors.forename: J
authors.initials: J
authors.lastname: Bonnedal
authors.forename: J
authors.initials: J
authors.lastname: Haggstrom
authors.forename: A
authors.initials: A
authors.lastname: Wistedt
authors.forename: V
authors.initials: V
authors.lastname: Fernandez
authors.forename: M C
authors.initials: MC
authors.lastname: Arendrup
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000453:epidemiology* / Q000382:microbiology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004271:DNA, Fungal / Q000235:genetics*; D004196:Disease Outbreaks; D005260:Female; D016469:Fungemia / Q000382:microbiology; D005821:Genetic Techniques; D005838:Genotype; D006757:Hospital Units; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013548:Sweden / Q000453:epidemiology
chemical_list: D004271:DNA, Fungal