title: Effect of glutaraldehyde treatment on stability of permeabilized Ochrobactrum anthropi SY509 in nitrate removal.
abstract: For practical application, the stability of permeabilized Ochrobactrum anthropi SY509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. As the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. However, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. Thus, wholecell immobilization using glutaraldehyde was performed, and 0.1% (v/v) glutaraldehyde was determined as the optimum concentration to maintain activity and increase the half-life. It was also found that 0.1% (v/v) glutaraldehyde reacted with 41.9% of the total amine residues on the surface of the cells during the treatment. As a result, the half-life of the permeabilized cells was increased from 90 to 210 days by glutaraldehyde treatment after permeabilization, and no cell viability was detected.
authors.affiliation: Graduate Program in Biochemical Engineering and Biotechnology Seoul National University, Seoul 151-744, Korea.
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Park
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.forename: Suk Soon
authors.initials: SS
authors.lastname: Choi
authors.forename: Young Je
authors.initials: YJ
authors.lastname: Yoo
mesh_terms: D001709:Biotechnology / Q000379:methods; D018914:Cells, Immobilized; D015169:Colony Count, Microbial; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005976:Glutaral / Q000494:pharmacology*; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009572:Nitrite Reductases / Q000378:metabolism; D020621:Ochrobactrum anthropi / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D010539:Permeability
chemical_list: D003432:Cross-Linking Reagents; D009566:Nitrates; D009572:Nitrite Reductases; D050901:Nitrate Reductase; D005976:Glutaral