[Establishing the method of collagen-gel droplet embedded culture drug sensitivity test with rat tail collagen].
OBJECTIVE
To explore the clinical application of collagen-gel droplet embedded-culture drug sensitivity test (CD-DST) in the malignant tumors.


METHODS
CD-DST was established with rat tail collagen. Three pancreatic cancer cell lines, surgical resection specimens including 15 cases of pancreatic cancer and 10 cases of gastrointestinal cancer were examined using CD-DST.


RESULTS
The overall achievement ratio of CD-DST for clinical tumor specimens was 80% (20/25). In vitro chemosensitivities of pancreatic carcinoma cells to 5-FU, gemcitabine and oxaliplatin were lower than those of gastrointestinal carcinoma.


CONCLUSIONS
CD-DST with rat tail collagen is a valuable chemosensitivity testing method for malignant tumors. It can be used to realize individualized anticancer chemotherapy.
Hu
Ya
Y
Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhou
Tao
T
Liao
Quan
Q
Zhang
Tai-Ping
TP
Chen
Ge
G
Zhao
Yu-Pei
YP
D000818:Animals; D003094:Collagen; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D005782:Gels; D006801:Humans; D008297:Male; D051381:Rats; D013623:Tail / Q000737:chemistry; D014407:Tumor Cells, Cultured
D005782:Gels; D003094:Collagen