[Three-step purification of preparative-scale antiCD20 (Fab')2].
OBJECTIVE
To establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime.


METHODS
AntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF.


RESULTS
Around 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100.


CONCLUSIONS
The three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.
Wang
Jin-Hong
JH
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Yang
Ming
M
Fan
Dong-Mei
DM
Xu
Yuan-Sheng
YS
Xiong
Dong-Sheng
DS
Yang
Chun-Zheng
CZ
D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D018951:Antigens, CD20 / Q000276:immunology*; D002845:Chromatography / Q000379:methods*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies; D018951:Antigens, CD20; D007140:Immunoglobulin Fab Fragments