[Effects of piperazine ferulate on connective tissue growth factor and extracellular matrix in TGF-beta1 induced mesangial cells].
OBJECTIVE
To observe the effects of piperazine ferulate (PF) on synthesis of extracellular matrix and expression of connective tissue growth factor (CTGF) in transforming growth factor-betal (TGF-beta1) induced rat glomerular mesangial cells (GMC), and to investigate its mechanism on the prevention of glomerulosclerosis.


METHODS
Rat HBZY-1 GMCs were cultured in vitro and divided into 5 groups: Normal control group, TGF-beta1-stimulated group, TGF-beta1 plus 50, 100, 200 ng/mL PF group. The level of CTGF protein expression induced by TGF-beta1 was measured by immunocytochemistry, The mRNA expression of CTGF was evaluated by quantitative real time fluroscent polymerase chain reaction. The expressions of fibronectin and collagen I were analyzed by enzyme linked immunosorbent assay (ELISA).


RESULTS
With the stimulation of TGF-beta1, the expression of of CTGF protein and mRNA, as well as fibronectin and collagen I were markly increased (P < 0.05), which then were decreased by the treatment of PF. (P < 0.05).


CONCLUSIONS
PF can partly inhibit synthesis of the extracellular matrix and CTGF. PF may take part in the prevention of glomerulosclerosis through the inhibition of CTGF expression.
Yin
Jian-ping
JP
Department of Nephrology, West China Hospital, Sichuan University, Chengdu, China.
Fu
Pinga
P
Xie
Xi-sheng
XS
Liu
Fang
F
Liu
Fei
F
Zhou
Li
L
D000818:Animals; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D003373:Coumaric Acids / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism*; D005923:Glomerulosclerosis, Focal Segmental / Q000517:prevention & control; D050527:Mesangial Cells / Q000166:cytology / Q000378:metabolism*; D000077489:Piperazine; D010879:Piperazines / Q000494:pharmacology*; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
D003373:Coumaric Acids; D010879:Piperazines; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D000077489:Piperazine; C004999:ferulic acid