Heterotrimeric coiled coils with core residue urea side chains.
We report several coiled coil heterotrimers with varying core residue buried polar groups, all with T(m) values >43 degrees C. Introduction of new synthetic side chain structures, including some terminating in monosubstituted ureas, diversifies the pool of viable core residue candidates. A study of core charge pairings demonstrates that, unlike dimeric systems, trimeric coiled coils do not tolerate guanidine-guanidine contacts, even in the presence of a compensating carboxylate. Overall, the roster of feasible coiled coil designs is significantly expanded.
Diss
Maria L
ML
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, USA.
Kennan
Alan J
AJ
D000595:Amino Acid Sequence; D002264:Carboxylic Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D019791:Guanidine / Q000737:chemistry; D006575:Heterocyclic Compounds, 3-Ring / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D011487:Protein Conformation; D014508:Urea / Q000737:chemistry*
D002264:Carboxylic Acids; D006575:Heterocyclic Compounds, 3-Ring; D007202:Indicators and Reagents; D009842:Oligopeptides; D014508:Urea; D019791:Guanidine