title: Interference in clinical chemistry assays by the hemoglobin-based oxygen carrier, Hemospan.
abstract: OBJECTIVE
  To evaluate interference by the hemoglobin-based oxygen carrier Hemospan on clinical laboratory assays.
  
  
  METHODS
  Interfering Hemospan concentrations were determined for general chemistry and cardiac marker analytes in pooled serum and the corresponding hemolysis index was calculated.
  
  
  RESULTS
  Hemospan did not interfere with 20 of 35 analytes. Hemospan produced a negative interference in serum creatinine, amylase, alkaline phosphatase, uric acid, and GGT assays and a positive interference in serum phosphate, LDH, iron, triglycerides, total protein, AST, cholesterol, magnesium, and albumin assays, and appeared to positively bias the serum cardiac troponin I (cTnI) assay only when cTnI is present in the sample.
  
  
  CONCLUSIONS
  We present a report of assays affected by Hemospan and the threshold concentrations for interference. This study highlights the importance of interference studies in understanding the effects of hemoglobin-based oxygen carriers on results reported by the clinical laboratory.
authors.affiliation: Department of Pathology, Clinical Chemistry Division, Johns Hopkins Medical Institutions, Meyer B-125 600 N. Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Cameron
authors.forename: Gregory
authors.initials: G
authors.lastname: Gerhardt
authors.forename: Michael
authors.initials: M
authors.lastname: Engelstad
authors.forename: Mark A
authors.initials: MA
authors.lastname: Young
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Norris
authors.forename: Lori J
authors.initials: LJ
authors.lastname: Sokoll
mesh_terms: D001774:Blood Chemical Analysis / Q000592:standards*; D001802:Blood Substitutes; D002624:Chemistry, Clinical / Q000379:methods / Q000592:standards*; D003951:Diagnostic Errors; D006454:Hemoglobins; D006801:Humans; D008301:Maleimides; D011092:Polyethylene Glycols; D015203:Reproducibility of Results
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D008301:Maleimides; C493836:maleimide-polyethylene glycol-modified hemoglobin, MP4; D011092:Polyethylene Glycols