Effect of barley and oat cultivars with different carbohydrate compositions on the intestinal bacterial communities in weaned piglets.
This experiment was aimed at comparing the intestinal microbial community composition in pigs fed hulled common barley supplemented with isolated barley mixed-linked beta-glucan, four hulless barley varieties and breeding lines with mixed-linked beta-glucan contents ranging from 41 to 84 g kg(-1) and different amylose/amylopectin ratios as well as two oat varieties. Seventy-two weaned piglets were allocated to one of nine diets composed of 81.5% cereal, 6% whey, 9% soy protein isolate and 3.5% minerals. After 15 days, pigs were sacrificed and ileum and colon contents were collected for quantitative real-time PCR (qPCR) and denaturing gradient gel electrophoresis to evaluate microbial communities. Shifts in intestinal microbial communities were observed with the hulless barley cultivars with a normal to high beta-glucan content and from normal starch toward either high-amylopectin or high-amylose starch. These hulless barleys had the lowest (P<0.05) microbial diversity, whereas oats had intermediate diversity compared with low-beta-glucan hulless cultivars and hulled varieties. Furthermore, hulless varieties favoured xylan- and beta-glucan-degrading bacteria whereas mixed-linked beta-glucan-supplemented hulled barley favoured lactobacilli. Numbers of lactobacilli decreased in the ileum of pigs fed hulless/high mixed-linked beta-glucan barley-based diets. Thus, cultivar differences in both the form and the quantity of carbohydrates affect gut microbiota in pigs, which provides information for future feeding strategies.
Pieper
Robert
R
Research Unit for Nutritional Physiology 'Oskar Kellner', Research Institute for the Biology of Farm Animals, Dummerstorf, Germany.
Jha
Rajesh
R
Rossnagel
Brian
B
Van Kessel
Andrew G
AG
Souffrant
Wolfgang B
WB
Leterme
Pascal
P
D000818:Animals; D018554:Avena / Q000737:chemistry*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D018407:Bacterial Physiological Phenomena; D015373:Bacterial Typing Techniques; D003106:Colon / Q000382:microbiology*; D015169:Colony Count, Microbial; D004032:Diet / Q000662:veterinary; D004040:Dietary Carbohydrates / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry*; D007085:Ilium / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011897:Random Allocation; D013552:Swine / Q000382:microbiology*; D014886:Weaning; D047071:beta-Glucans / Q000378:metabolism
D004040:Dietary Carbohydrates; D012336:RNA, Ribosomal, 16S; D047071:beta-Glucans