title: [Role of phospholipase C-gamma1 signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells].
abstract: OBJECTIVE
  To explore the role of phospholipase C-gamma1 (PLC-gamma1) signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells.
  
  
  METHODS
  PC12 cells were exposed to 50 micromol/L H(2)O(2) after pretreatment with 10 micromol/L U73122, a specific PLC-gamma1 inhibitor. Hoechst/PI double staining was performed to observe the morphological changes of the cells under light microscope. MTT assay was used to evaluate the cell viability, and the percentage of apoptotic cells was analyzed by flow cytometry. DNA fragmentation assay was carried out to characterize the cell apoptosis.
  
  
  RESULTS
  After inhibition of the PLC-gamma1 signaling pathway with 10 micromol/L U73122, PC12 cells showed obvious apoptotic morphology, the viable cells decreased significantly, and the percentage of apoptotic cells rose to 35.7%. PC12 cells treated with U73122 presented with a distinct DNA ladder on electrophoresis resulting from DNA cleavage in the apoptotic cells.
  
  
  CONCLUSIONS
  PLC-gamma1 signaling pathway plays an important protective role in H(2)O(2)-induced PC12 cell apoptosis.
authors.affiliation: Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Yuan
authors.forename: Di
authors.initials: D
authors.lastname: Lu
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Guang-Xue
authors.initials: GX
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
authors.forename: Shen-Qiu
authors.initials: SQ
authors.lastname: Luo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004963:Estrenes / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016716:PC12 Cells; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011760:Pyrrolidinones / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction
chemical_list: D004963:Estrenes; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D006861:Hydrogen Peroxide; D051966:Phospholipase C gamma