title: [The primary application of direct rapid immunohistochemical test to rabies diagnosis in China].
abstract: OBJECTIVE
  Evaluation of the direct rapid immumohistochemical test (DRIT) for laboratory surveillance of rabies.
  
  
  METHODS
  72 brain specimens of domestic dogs or patients collected from Guizhou, Guangxi, Hunan, Anhui, Jiangsu and Yunnan provinces were detected by conventional methods including Direct Fluorescent-antibody Assay (DFA) and Reverse Transcription Polymerase Chain Reaction (RT-PCR), and by DRIT which was newly developed in the Rabies Section of the Centers for Disease Control and Prevention in the United States. The sensitivity and specificity of DRIT were evaluated by compare of the three results. By analysis of the index including cost of experiment, technique requirement and so on, the advancement and applicability of DRIT were discussed.
  
  
  RESULTS
  Compared with DFA and RT-PCR, DRIT will be more applicable for laboratories with limited funds and weak techniques because of its lower cost needed and simpler techniques required while its sensitivity and specificity are equal to the other two methods.
  
  
  CONCLUSIONS
  DRIT is more valuable in rabies diagnosis and more applicable for extension and popularization in rabies laboratory surveillance in local CDC.
authors.affiliation: Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Tao
authors.forename: Michael
authors.initials: M
authors.lastname: Niezgoda
authors.forename: Jia-Liang
authors.initials: JL
authors.lastname: Du
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology; D002681:China / Q000453:epidemiology; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D004285:Dogs; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D015995:Prevalence; D011818:Rabies / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*