TRPM5-expressing microvillous cells in the main olfactory epithelium.
BACKGROUND
The main olfactory epithelium (MOE) in the nasal cavity detects a variety of air borne molecules that provide information regarding the presence of food, predators and other relevant social and environmental factors. Within the epithelium are ciliated sensory neurons, supporting cells, basal cells and microvillous cells, each of which is distinct in morphology and function. Arguably, the least understood, are the microvillous cells, a population of cells that are small in number and whose function is not known. We previously found that in a mouse strain in which the TRPM5 promoter drives expression of the green fluorescent protein (GFP), a population of ciliated olfactory sensory neurons (OSNs), as well as a population of cells displaying microvilli-like structures is labeled. Here we examined the morphology and immunocytochemical properties of these microvillous-like cells using immunocytochemical methods.


RESULTS
We show that the GFP-positive microvillous cells were morphologically diversified and scattered throughout the entire MOE. These cells immunoreacted to an antibody against TRPM5, confirming the expression of this ion channel in these cells. In addition, they showed a Ca2+-activated non-selective cation current in electrophysiological recordings. They did not immunoreact to antibodies that label cell markers and elements of the transduction pathways from olfactory sensory neurons and solitary chemosensory cells of the nasal cavity. Further, the TRPM5-expressing cells did not display axon-like processes and were not labeled with a neuronal marker nor did trigeminal peptidergic nerve fibers innervate these cells.


CONCLUSIONS
We provide morphological and immunocytochemical characterization of the TRPM5-expressing microvillous cells in the main olfactory epithelium. Our data demonstrate that these cells are non-neuronal and in terms of chemosensory transduction do not resemble the TRPM5-expressing olfactory sensory neurons and nasal solitary chemosensory cells.
Lin
Weihong
W
Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA. weihong@umbc.edu
Ezekwe
Ejiofor A D
EA
Zhao
Zhen
Z
Liman
Emily R
ER
Restrepo
Diego
D
D000818:Animals; D015415:Biomarkers; D015220:Calcium Channels / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008871:Microvilli / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D018035:Receptors, Odorant / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D015222:Sodium Channels / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D050053:TRPM Cation Channels / Q000096:biosynthesis* / Q000235:genetics*; D014276:Trigeminal Nerve / Q000166:cytology / Q000502:physiology
D015415:Biomarkers; D015220:Calcium Channels; D018035:Receptors, Odorant; D015222:Sodium Channels; D050053:TRPM Cation Channels; C498617:Trpm5 protein, mouse; D049452:Green Fluorescent Proteins