The potential role of suppressors of cytokine signaling in the attenuation of inflammatory reaction and alveolar bone loss associated with apical periodontitis.
Inflammatory cytokines contribute to periapical tissue destruction. Their activity is potentially regulated by suppressors of cytokine signaling (SOCS), which downregulate signal transduction as part of an inhibitory feedback loop. We investigated the expression of the cytokines tumor necrosis factor alpha (TNF-alpha); interleukin (IL)-10 and RANKL; and SOCS-1, -2, and -3 by real-time polymerase chain reaction in 57 periapical granulomas and 38 healthy periapical tissues. Periapical granulomas exhibited significantly higher SOCS-1, -2, and -3, TNF-alpha, IL-10, and RANKL messenger RNA levels when compared with healthy controls. Significant positive correlations were found between SOCS1 and IL-10 and between SOCS3 and IL-10. Significant inverse correlations were observed between SOCS1 and TNF-alpha, SOCS1 and RANKL, and SOCS3 and TNF-alpha. Increased SOCS-1, -2, and -3 messenger RNA levels in periapical granulomas may be related to the downregulation of inflammatory cytokines in these lesions; therefore, SOCS molecules may play a role in the dynamics of periapical granulomas development.
Menezes
Renato
R
Department of Biological Sciences, Bauru Dental School, University of São Paulo Bauru, SP, Brazil.
Garlet
Thiago Pompermaier
TP
Trombone
Ana Paula Fávaro
AP
Repeke
Carlos Eduardo
CE
Letra
Ariadne
A
Granjeiro
José Mauro
JM
Campanelli
Ana Paula
AP
Garlet
Gustavo Pompermaier
GP
D000293:Adolescent; D000328:Adult; D016301:Alveolar Bone Loss / Q000276:immunology*; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010484:Periapical Granuloma / Q000276:immunology; D010485:Periapical Periodontitis / Q000276:immunology*; D010486:Periapical Tissue / Q000276:immunology; D053245:RANK Ligand / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000276:immunology; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000032:analysis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D055815:Young Adult
D053245:RANK Ligand; D012333:RNA, Messenger; C108944:SOCS1 protein, human; C106643:SOCS2 protein, human; C106644:SOCS3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10