title: cAMP receptor protein from escherichia coli as a model of signal transduction in proteins--a review.
abstract: In Escherichia coli, cyclic AMP receptor protein (CRP) is known to regulate the transcription of about 100 genes. The signal to activate CRP is the binding of cyclic AMP. It has been suggested that binding of cAMP to CRP leads to a long-distance signal transduction from the N-terminal cAMP-binding domain to the C-terminal domain of the protein, which is responsible for interaction with specific sequences of DNA. The signal transduction plays a crucial role in the activation of the protein. The most sophisticated spectroscopic techniques, other techniques frequently used in structural biochemistry, and site-directed mutagenesis have been used to investigate the details of cAMP-mediated allosteric control over CRP conformation and activity as a transcription factor. The aim of this review is to summarize recent works and developments pertaining to cAMP-dependent CRP signal transduction in E. coli.
authors.affiliation: Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland. ewelina.fic@uj.edu.pl
authors.forename: E
authors.initials: E
authors.lastname: Fic
authors.forename: P
authors.initials: P
authors.lastname: Bonarek
authors.forename: A
authors.initials: A
authors.lastname: Gorecki
authors.forename: S
authors.initials: S
authors.lastname: Kedracka-Krok
authors.forename: J
authors.initials: J
authors.lastname: Mikolajczak
authors.forename: A
authors.initials: A
authors.lastname: Polit
authors.forename: M
authors.initials: M
authors.lastname: Tworzydlo
authors.forename: M
authors.initials: M
authors.lastname: Dziedzicka-Wasylewska
authors.forename: Z
authors.initials: Z
authors.lastname: Wasylewski
mesh_terms: D000494:Allosteric Regulation; D000242:Cyclic AMP / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D015398:Signal Transduction
chemical_list: D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; C481662:crp protein, E coli; D000242:Cyclic AMP