Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection.
Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.
Bohn
Yu-Sing Tammy
YS
Klinische Forschergruppe, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse, Hannover, Germany.
Brandes
Gudrun
G
Rakhimova
Elza
E
Horatzek
Sonja
S
Salunkhe
Prabhakar
P
Munder
Antje
A
van Barneveld
Andrea
A
Jordan
Doris
D
Bredenbruch
Florian
F
Häussler
Susanne
S
Riedel
Kathrin
K
Eberl
Leo
L
Jensen
Peter Østrup
PØ
Bjarnsholt
Thomas
T
Moser
Claus
C
Hoiby
Niels
N
Tümmler
Burkhard
B
Wiehlmann
Lutz
L
D000818:Animals; D001692:Biological Transport; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D051379:Mice; D008809:Mice, Inbred C3H; D016296:Mutagenesis; D009504:Neutrophils / Q000382:microbiology; D010619:Phenazines / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000382:microbiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010619:Phenazines; C103855:PilY1 protein, Pseudomonas aeruginosa; D020033:Protein Isoforms; D011804:Quinolines; D036781:Fimbriae Proteins