title: Scalable production of pancreatic islet progenitors for diabetes cell therapies.
abstract: Embryonic stem cells (ESCs) can be an inexhaustible source of islet cells for transplantation. Previously published protocols have been characterized by low differentiation efficiency. In this study, we developed a scalable system for the growth and differentiation of hESCs towards pancreatic islets. Our results showed that hESCs can be grown on microcarriers to a larger scale and directed to differentiate into pancreatic progenitor endoderm cells. This culture system would represent an economical differentiation protocol that can be scaled-up to meet the demand in islet transplantation.
authors.affiliation: Department of Chemical and Biological Engineering, State University of New York, USA.
authors.forename: L T
authors.initials: LT
authors.lastname: Lock
authors.forename: E S
authors.initials: ES
authors.lastname: Tzanakakis
mesh_terms: D003920:Diabetes Mellitus / Q000628:therapy*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D010865:Pilot Projects; D013234:Stem Cells / Q000166:cytology
chemical_list: D007328:Insulin