[Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish].
OBJECTIVE
To study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish.


METHODS
In situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time.


RESULTS
Nspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head.


CONCLUSIONS
Nspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.
Wu
Xu-Dong
XD
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zhang
Min
M
Gong
Yan-Hua
YH
Qiang
Bo-Qin
BQ
Yuan
Jian-Gang
JG
Meng
An-Ming
AM
Peng
Xiao-Zhong
XZ
D000595:Amino Acid Sequence; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000254:growth & development / Q000378:metabolism; D063150:Polycomb Repressive Complex 1; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D012097:Repressor Proteins; D029961:Zebrafish Proteins; C549851:pcgf1 protein, Danio rerio; D063150:Polycomb Repressive Complex 1