title: Improvement of cardiac function and remodeling by transplanting adipose tissue-derived stromal cells into a mouse model of acute myocardial infarction.
abstract: BACKGROUND
  We investigated the effect of adipose tissue-derived stromal cells (ADSC) therapy on cardiac contractility and remodeling in the C57BL/6 mouse model of acute myocardial infarction (AMI).
  
  
  METHODS
  30 adult male C57BL/6 mice were randomized into 2 groups, namely, AMI+media (control, n=15) and AMI+ADSC (n=15). AMI was produced by left anterior descending coronary artery ligation. After AMI induction, 1 x 10(6) ADSC or media were intramyocardially injected and the results compared. Echocardiographic and histological analyses of surviving mice (n=20) were conducted. Echocardiography was performed before cell implantation and 2 weeks after transplantation.
  
  
  RESULTS
  LVEF and FS improved in the ADSC group compared to the control (P<0.01). LVEDD in the ADSC group decreased slightly from 4.65+/-0.63 mm to 4.14+/-0.53 mm compared to the control, but there was no statistical difference (P=0.072). LVESD decreased significantly in the ADSC group (P<0.05). A significant difference in scar formation and infarct size was observed between the ADSC and control group 2 weeks after AMI (P<0.05). ADSC were observed to migrate into injured sites and integrate into scar areas and increased vascular density in the infarct site compared to control group (P<0.05). Additionally, some transplanted ADSC expressed the endothelial marker.
  
  
  CONCLUSIONS
  Echocardiography and histological analysis revealed that improvement in cardiac function and ventricular remodeling was better in the ADSC group than in the control. This suggests that ADSC is a good candidate for cell therapy in cardiovascular disease.
authors.affiliation: Department of Cardiology, College of Medicine, Dong-A University, Busan, South Korea.
authors.forename: Long Hao
authors.initials: LH
authors.lastname: Yu
authors.forename: Moo Hyun
authors.initials: MH
authors.lastname: Kim
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Park
authors.forename: Kwang Soo
authors.initials: KS
authors.lastname: Cha
authors.forename: Young Dae
authors.initials: YD
authors.lastname: Kim
authors.forename: Mei Lian
authors.initials: ML
authors.lastname: Quan
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Rho
authors.forename: Su Yeoung
authors.initials: SY
authors.lastname: Seo
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
mesh_terms: D002001:Adipose Tissue, Brown / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D017690:Cell Transplantation / Q000379:methods*; D002921:Cicatrix / Q000473:pathology; D004195:Disease Models, Animal; D004452:Echocardiography; D006334:Heart Function Tests; D007150:Immunohistochemistry; D008222:Lymphokines; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009200:Myocardial Contraction / Q000502:physiology; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000637:transplantation*; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: D015415:Biomarkers; D008222:Lymphokines; C092591:fibrosin