Differential expression of HSP70 mRNA in the mouse brain after treatment with geldanamycin.
OBJECTIVE
The effect of geldanamycin (GA) on the brain is poorly understood. The induction of specific heat shock protein (HSP) isoforms by GA has not been studied in detail. The aim of this work was to identify the HSP70 genes that become up-regulated in the mouse brain after GA administration.


METHODS
The brains of male hsp 70.1 knockout and littermate mice were obtained after GA injection into the lateral ventricle. The mice were killed 6 hours later to investigate the levels of hsp 70.1 and hsp 70.3 using semi-quantitative reverse transcriptase polymerase chain reactions.


RESULTS
GA infusion into the mouse brain led to the up-regulation of hsp 70.1.


CONCLUSIONS
Further work is needed to more clearly define the role of those genes in the clinical effects of GA.
Kwon
Hyung-Min
HM
Department of Neurology, Seoul National University Boramae Hospital, Chongno-gu, Seoul, Republic of Korea.
Kim
Yong-Ju
YJ
Ryu
Sun
S
Yang
Seung-In
SI
Lee
Seung-Hoon
SH
Yoon
Byung-Woo
BW
D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology*; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D007276:Injections, Intraventricular; D047029:Lactams, Macrocyclic / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016227:Benzoquinones; D018840:HSP70 Heat-Shock Proteins; C498550:Hsp70.3 protein, mouse; D047029:Lactams, Macrocyclic; C081884:heat-shock protein 70.1; C001277:geldanamycin