title: Structure of the wild-type human BCL6 POZ domain.
abstract: BCL6 is a transcriptional repressor that is overexpressed in diffuse large B-cell lymphoma and follicular lymphoma. The N-terminal POZ domain of BCL6 interacts with transcriptional corepressors and targeting these associations is a promising therapeutic strategy. Previous structural studies of the BCL6 POZ domain have used a mutant form because of the low solubility of the wild-type recombinant protein. A method for the purification and crystallization of the wild-type BCL6 POZ domain is described and the crystal structure to 2.1 A resolution is reported. This will be relevant for the design of therapeutics that target BCL6 POZ-domain interaction interfaces.
authors.affiliation: Molecular Cell Biology Research Group, Garstang/Astbury Buildings, Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, England.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Stead
authors.forename: Gareth O
authors.initials: GO
authors.lastname: Rosbrook
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Hadden
authors.forename: Chi H
authors.initials: CH
authors.lastname: Trinh
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Carr
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Wright
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D051560:Proto-Oncogene Proteins c-bcl-6; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D011994:Recombinant Proteins