title: [Determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food by high performance anion exchange chromatography].
abstract: OBJECTIVE
  To develop a method for analysis of eight kinds of monosaccharides using high performance anion exchange chromatography.
  
  
  METHODS
  Amino PAC PA-10 (2 x 250 mm) column with a pulse amperometric detector with an Au working electrode and an Ag/AgCl reference electrode was applied to determine the monosaccharides.
  
  
  RESULTS
  The linear range was 0.1-5.0 microg/mL and the detection limits were 2-26 microg/L. The relative standard derivation for retain time and area of standard solution were 0.34%-7.42% and 0.02%-2.16% respectively. The satisfactory results were obtained for analysis of honey and polysaccharide hydrolyzate of functional food with the proposed method. The average recoveries were 81.4%-122.0%. The method was sensitive and with high precision.
  
  
  CONCLUSIONS
  This method was available for determination of monosaccharides in honey and polysaccharide hydrolyzate of functional food.
authors.affiliation: Department of Sanitary Technology, West China School of Pulblic Health, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Zou
authors.forename: Shui
authors.initials: S
authors.lastname: Jiang
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zeng
authors.forename: Ci-ling
authors.initials: CL
authors.lastname: Yan
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006722:Honey / Q000032:analysis*; D006868:Hydrolysis; D009005:Monosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides