Enzyme-linked immunosorbent assay method for detection of cytomegalovirus strain-specific antibody responses.
Reliable methods for the detection of cytomegalovirus (CMV) strain-specific serological responses are lacking. We describe a simple and reliable enzyme-linked immunosorbent assay method developed to detect antibodies against the polymorphic epitopes within the two envelope glycoproteins of CMV, glycoproteins H and B. This assay is useful for the detection of serologic responses to CMV strains and the identification of CMV reinfections.
Novak
Zdenek
Z
Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, Alabama 35233, USA. zdnovak@peds.uab.edu
Ross
Shannon A
SA
Patro
Raj Kumar
RK
Pati
Sunil Kumar
SK
Reddy
Meera K
MK
Purser
Misty
M
Britt
William J
WJ
Boppana
Suresh B
SB
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C071440:glycoprotein H, Cytomegalovirus