title: Two-dimensional difference gel electrophoresis analysis of Streptococcus uberis in response to mutagenesis-inducing ciprofloxacin challenge.
abstract: In Streptococcus uberis, the fluoroquinolone antibiotic ciprofloxacin induces a mutagenic response that is distinct from the SOS paradigm. Two-dimensional differential gel electrophoresis was employed to investigate the effect of ciprofloxacin exposure on the proteome of S. uberis. Twenty-four protein spots exhibiting differential expression (p < 0.05) were identified as enzymes with potential role in oxidative stress, NADH generation and nucleotide biosynthesis. We suggest that these metabolic changes provide S. uberis means to stimulate mutagenesis and adaptation.
authors.affiliation: Institute of Biotechnology, and Department of Basic Veterinary Sciences, University of Helsinki, Finland.
authors.forename: Marjo
authors.initials: M
authors.lastname: Poutanen
authors.forename: Emilia
authors.initials: E
authors.lastname: Varhimo
authors.forename: Nisse
authors.initials: N
authors.lastname: Kalkkinen
authors.forename: Antti
authors.initials: A
authors.lastname: Sukura
authors.forename: Pekka
authors.initials: P
authors.lastname: Varmanen
authors.forename: Kirsi
authors.initials: K
authors.lastname: Savijoki
mesh_terms: D001483:Base Sequence; D002232:Carbocyanines / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009711:Nucleotides / Q000737:chemistry; D018384:Oxidative Stress; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013291:Streptococcus / Q000378:metabolism*
chemical_list: D002232:Carbocyanines; D009711:Nucleotides; C089187:cyanine dye 3; C085321:cyanine dye 5; D002939:Ciprofloxacin