Cardioprotection by Guanxin II in rats with acute myocardial infarction is related to its three compounds.
OBJECTIVE
We tested the hypothesis that cardioprotection afforded by traditional Chinese Guanxin II (GXII) formula is related to absorbed bioactive compounds (ABCs).


METHODS
Sprague-Dawley rats with acute myocardial infarction (AMI) were induced by coronary occlusion. ABCs including ferulic acid (F), hydroxyl safflor yellow A (A), tanshinol (T), protocatechualdehyde (P) and paeoniflorin (E) were measured in blood after oral GXII. The effects of GXII and FATPE, alone and in combination, and of some components of FATPE on infarct size, myocardial apoptosis and caspase-3 activity were determined. Myocardial blood flow (MBF) in AMI rat was detected 2h after oral GXII and FAT.


RESULTS
FATPE was found in rat blood. FAT was similar to FATPE and GXII in decreasing infarct size, myocardial apoptosis and caspase-3 activity of AMI. Both FAT and GXII were similar in increasing of MBF.


CONCLUSIONS
GXII and FAT protect the heart from ischemic injury by increasing MBF, and decrease infarct size by inhibiting myocardial apoptosis and caspase-3 activity. These findings provide a potential cardioprotective cocktail.
Huang
Xi
X
Laboratory of Ethnopharmacology and Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. tcmhuangx59@163.com
Qin
Feng
F
Zhang
Hong-Min
HM
Xiao
Hong-Bin
HB
Wang
Long-Xin
LX
Zhang
Xue-Ya
XY
Ren
Ping
P
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002316:Cardiotonic Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D003326:Coronary Circulation / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; C534196:guan xin II; D053148:Caspase 3