[Effects of Newcastle disease virus on the mitochondria of human gastric carcinoma BGC-823 cells].
OBJECTIVE
To explore changes in structure and function of the mitochondria of human gastric carcinoma BGC-823 cells after Newcastle disease virus (NDV) infection.


METHODS
Electron microscopy was applied to observe the structure of mitochondria; Rhodamine 123 staining was used to determine the mitochondrial membrane potential; the activity of Na(+)-K(+)-ATPase and Ca(2+)-ATPase were also determined and the release of cytochrome C was detected by Western blotting.


RESULTS
The structure of mitochondria in the tumor cells infected with NDV changed distinctly. In the infected group the activity of mitochondrial Na(+)-K(+)-ATPase and Ca(2+)-ATPase significantly declined (P < 0.01), and compared with control cells, mitochondrial trans-membrane potential was decreased. NDV infection induced the decrease of cytochrome C levels.


CONCLUSIONS
The effects of NDV infection on the structure and functions of mitochondria of human gastric carcinoma BGC-823 cells might play a role in the oncolysis of NDV.
Liu
Kai-Yang
KY
Department of Microbiology of Hebei North University, Zhangjiakou, Hebei province 075000, China. kaiyang1999@yahoo.com.cn
Qu
Jian-Guo
JG
Liu
Jin-Jun
JJ
Liu
Fang
F
D000818:Animals; D002277:Carcinoma / Q000201:enzymology / Q000378:metabolism / Q000821:virology*; D045744:Cell Line, Tumor; D002642:Chick Embryo; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism / Q000821:virology*; D009521:Newcastle Disease / Q000201:enzymology / Q000378:metabolism / Q000821:virology*; D009522:Newcastle disease virus / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013274:Stomach Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000821:virology*
D045304:Cytochromes c; D000254:Sodium-Potassium-Exchanging ATPase