title: [PrP protein can bind to protein kinase CK2 both in native and recombinant forms in vitro].
abstract: To explore the possible molecular interaction between CK2 and PrP, the full length sequences of human CK2alpha and CK2beta genes were amplified with RT-PCR using the mRNA from cell line SH-SY5Y as the template, and then the fusion proteins HIS-CK2alpha and GST-HIS-CK2beta were expressed in E. coli. The interaction between CK2 and PrP was evaluated with immunoprecipitation and pull-down assays. The results demonstrated that recombinant PrP bound specifically with CK2alpha, but not with CK2beta. The native CK2 and PrP in the hamster brains interacted each other, forming protein complexes. The domain responsible for interacting with CK2alpha was located at the C-terminal segment of PrP (residues 90-231). This study proposed reliable experimental data for the molecular interaction between PrP and CK2alpha, both in recombinant and native categories. These results supply scientific clues for further assessing the potential biological significance of the interaction of PrP with CK2 and possible role of CK2 in the pathogenesis of prion diseases.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Gao
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Lei
authors.forename: Bing
authors.initials: B
authors.lastname: Shan
authors.forename: Chen-Fang
authors.initials: CF
authors.lastname: Dong
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Wang
authors.forename: Song
authors.initials: S
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000737:chemistry* / Q000502:physiology; D006224:Cricetinae; D006801:Humans; D047468:Immunoprecipitation; D010766:Phosphorylation; D017096:Prion Diseases / Q000209:etiology; D011328:Prions / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011328:Prions; D011994:Recombinant Proteins; D047390:Casein Kinase II