Living bilayered human skin equivalent: promising potentials for wound healing.
The angiogenic potential of native skin (NS), keratinocytes single skin equivalent (SSE-K), fibroblasts single skin equivalent (SSE-F) and bilayered skin equivalent secreting angiogenic growth factors such as transforming growth factor beta1 (TGF-beta1), vascular endothelial growth factor (VEGF), keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) in the in vitro systems at 24, 48, 72 hours and 7 days was compared using Enzyme-Linked Immunosorbent Assay (ELISA). Bilayered skin equivalent exhibit highest release of growth factors within 24 hours to 7 days of culture compared to NS, SSE-K and SSE-F. This proved the potential of bilayered skin equivalent in producing and sustaining growth factors release to enhance angiogenesis, fibroblasts proliferation, matrix deposition, migration and growth of keratinocytes.
Mazlyzam
A L
AL
Department of Physiology, Medical Centre, Universiti Kebangsaan Malaysia (UKM).
Aminuddin
B S
BS
Saim
L
L
Ruszymah
B H I
BH
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D049109:Cell Proliferation; D010450:Endopeptidases; D004797:Enzyme-Linked Immunosorbent Assay; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D051523:Fibroblast Growth Factor 7 / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology*; D018093:Gelatinases; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D008565:Membrane Proteins; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012697:Serine Endopeptidases; D053773:Transforming Growth Factor beta1 / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*; D000099167:Fibroblast Activation Protein Alpha
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D016222:Fibroblast Growth Factor 2; D051523:Fibroblast Growth Factor 7; D018093:Gelatinases; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D000099167:Fibroblast Activation Protein Alpha