title: Plant extracts as natural amoebicidal agents.
abstract: Strains of Acanthamoeba sp. constitute a factor contributing to the occurrence of chronic granulomatous amoebic encephalitis, keratitis, pneumonia, as well as inflammations of other organs. Treatment of these diseases is very difficult and not always effective. A majority of these infections have been fatal. The aim of our study was to examine the amoebicidal or amoebistatic activity of plant extracts from Rubus chamaemorus, Pueraria lobata, Solidago virgaurea and Solidago graminifolia. For the purpose of isolation of pharmacologically active substances, we used the aboveground parts of plants, together with flowers, roots and leaves. It was established that extracts from S. virgauera, P. lobata and R. chamaemorus displayed chemotherapeutic properties in vitro in concentrations of approximately 0.01-0.05 mg extract/mL, i.e., in concentrations of 0.350 microg/mL expressed in ellagic acid for R. chamaemorus and 0.053 microg/mL expressed in puerarin for P. lobata. Therapeutic index values is 3.5-20. As a result of in vivo experiments, it was found out that, following therapy using the extracts, animals infected with Acanthamoeba sp. survived for an extended period (2.5-3 times longer). It was determined that plant extracts may be used both externally and internally in the case of a combined therapy for acanthamoebiasis. The tested extracts are not toxic for animals.
authors.affiliation: Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry Street, Poznań, 61-701, Poland. mderda@ump.edu.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Derda
authors.forename: Edward
authors.initials: E
authors.lastname: Hadaś
authors.forename: Barbara
authors.initials: B
authors.lastname: Thiem
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects*; D000563:Amebicides / Q000494:pharmacology*; D000818:Animals; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry*; D027824:Rosaceae / Q000737:chemistry*; D036624:Solidago / Q000737:chemistry*
chemical_list: D000563:Amebicides; D010936:Plant Extracts