No genomic aberrations in Langerhans cell histiocytosis as assessed by diverse molecular technologies.
The etiology of Langerhans cell histiocytosis (LCH), a disease characterized by uncontrolled proliferation of Langerhans cells, is unknown. Although some believe that LCH is reactive, others support a neoplastic origin. We tested the hypothesis that LCH is neoplastic by investigating potential consistent chromosomal aberrations in LCH cells. We used multiparameter DNA flow cytometry to analyze the DNA ploidy LCH cells in 20 cases, performed karyotype analysis in 31 cases, array-based comparative genomic hybridization (arrayCGH) and single nucleotide polymorphism (SNP) arrays with DNA from flow-sorted CD1a-positive and CD1a-negative cells in 19 cases. Ploidy analysis revealed diploid DNA content in all cases. The karyotype of all patients analyzed was normal, excluding the presence of balanced translocations. ArrayCGH and SNP arrays did not show genome abnormalities. Despite positive TP53 protein immunohistochemical staining, sequencing of exon 5 to 8 of p53 gene showed no alterations in 7 cases. This study strongly suggests that gross chromosomal abnormalities do not cause LCH. Although we cannot exclude cryptic point mutations in as yet unidentified genes, this study of 72 LCH cases shows that LCH may be the result of restricted oligoclonal stimulation rather than unlimited neoplastic proliferation. (c) 2008 Wiley-Liss, Inc.
da Costa
Cristiana E T
CE
Department of Pediatrics, Immunology Laboratory, Leiden University Medical Center, Leiden, The Netherlands.
Szuhai
Karoly
K
van Eijk
Ronald
R
Hoogeboom
Manja
M
Sciot
Raphael
R
Mertens
Fredrik
F
Björgvinsdóttir
Helga
H
Debiec-Rychter
Maria
M
de Krijger
Ronald R
RR
Hogendoorn
Pancras C W
PC
Egeler
R Maarten
RM
Annels
Nicola E
NE
D000293:Adolescent; D000328:Adult; D000368:Aged; D018949:Antigens, CD1 / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D004252:DNA Mutational Analysis; D005260:Female; D005434:Flow Cytometry; D016158:Genes, p53; D006646:Histiocytosis, Langerhans-Cell / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007223:Infant; D007621:Karyotyping; D007801:Langerhans Cells / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide
D018949:Antigens, CD1; C419393:CD1a antigen