[Production of carotene and lycopene by mutants of Streptomyces globisporus 1912 cultivated on mealy media].
Carotenoids produced by strains 4Crt and 4Lcp, spontaneous mutants oflandomycin E producer Streptomyces globisporus 1912 with activated cryptic genes of carotenogenesis, were identified, and their quantitative output was studied after growth of Streptomycete cultures on different mealy media in shake flasks. On the basis of chromatographic mobility and maxima of absorption spectra of purified pigments they were related to lycopene, beta- and phi-carotene (isorenieratene). It was shown that strain 4Crt synthesizes both carotenoids, while its spontaneous mutant 4Lcp--only lycopene. The greatest output of lycopene (27.24 mg/l) was observed after cultivation of 4Lcp strain on soy-corn medium.
Holembiovs'ka
S L
SL
Matseliukh
B P
BP
D001431:Bacteriological Techniques / Q000379:methods*; D002338:Carotenoids / Q000096:biosynthesis*; D002855:Chromatography, Thin Layer; D003470:Culture Media; D000077276:Lycopene; D009154:Mutation; D013025:Glycine max; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D003313:Zea mays
D003470:Culture Media; D002338:Carotenoids; D000077276:Lycopene