Arthrobacter oxydans as a biocatalyst for purine deamination.
Deaminases are enzymes that catalyze the hydrolysis of amino groups of nucleosides or their bases. Because these enzymes play important roles in nucleotide metabolism, they are relevant targets in anticancer and antibacterial therapies. Mammalian deaminases are commercially available but the use of bacterial whole cells, especially as biocatalysts, is continuously growing because of their economical benefits. Moreover, deaminases are useful for the preparative chemoenzymatic transformation of nucleoside and base analogues into a variety of derivatives. The purine deaminase activities of Arthrobacter oxydans, a gram-positive bacterium utilized widely in bioremediation, were studied. The presence of adenosine, adenine and guanine deaminases was demonstrated and some purine bases and nucleosides were analyzed as substrates. Using A. oxydans whole cells as the biocatalyst, different purine compounds such as the anti-HIV, 2',3'-dideoxyinosine (73%, 2 h) were obtained.
Médici
Rosario
R
Biotransformation Laboratory, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
Lewkowicz
Elizabeth S
ES
Iribarren
Adolfo M
AM
D000243:Adenosine Deaminase / Q000378:metabolism; D000619:Aminohydrolases / Q000378:metabolism*; D019380:Anti-HIV Agents / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D003641:Deamination; D016049:Didanosine / Q000378:metabolism; D004798:Enzymes; D006148:Guanine Deaminase / Q000378:metabolism; D011684:Purine Nucleosides / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D013379:Substrate Specificity
D019380:Anti-HIV Agents; D004798:Enzymes; D011684:Purine Nucleosides; D011687:Purines; D000619:Aminohydrolases; C018774:adenine deaminase; D006148:Guanine Deaminase; D000243:Adenosine Deaminase; D016049:Didanosine