DNA markers for Portuguese olive oil fingerprinting.
The certification of olive oil has led to the definition of Protected Denomination of Origin (PDO) producing regions in European countries. PDO products should be protected, and a solution could be by using DNA fingerprinting. In this work we evaluate the efficiency of RAPD, ISSR, and SSR molecular markers for olive oil varietal identification and their possible use in certification purposes. Twenty-three Portuguese olive oil samples (11 obtained monovarietal and 12 purchased commercial oils) were screened by means of two RAPD, four ISSR, and four SSR markers. The quality of amplified products was used to evaluate the reproducibility and the level of polymorphism. Principal component analysis was performed with DCENTER using unweighted pair group mathematical average (UPGMA) that allowed group formation according to olive oil varietal geographic origin.
Martins-Lopes
Paula
P
Institute of Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro, P.O. Box 1013, 5001-801 Vila Real, Portugal. plopes@utad.pt
Gomes
Sónia
S
Santos
Elisabete
E
Guedes-Pinto
Henrique
H
D016172:DNA Fingerprinting; D005819:Genetic Markers; D018895:Microsatellite Repeats; D031658:Olea / Q000737:chemistry / Q000235:genetics*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D011174:Portugal; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers; D000069463:Olive Oil; D010938:Plant Oils