Characterization of Propionibacterium acnes isolates from sarcoid and non-sarcoid tissues with special reference to cell invasiveness, serotype, and trigger factor gene polymorphism.
Sarcoidosis is a systemic granulomatous disease of unknown etiology. Propionibacterium acnes is the only microorganism so far isolated from sarcoid lesions. To examine whether P. acnes isolates from sarcoid tissues differ from those obtained from non-sarcoid tissues, we studied cell invasiveness, serotype, and polymorphisms of the P. acnes trigger factor protein and the two invasion-associated proteins (named PAmce and PAp60) in 35 P. acnes isolates from sarcoid lymph nodes and 127 isolates from non-sarcoid tissues. Most of the serotype I isolates (79/112; 71%), but none of the serotype II isolates (0/50) were cell-invasive. Two prominent types of trigger factors, one with and one without a 15 amino acid-residue deletion, corresponded to serotype II and serotype I, respectively. Non-invasive isolates had genomic mutations that caused more than one amino acid change in either the PAmce or PAp60 gene, with four exceptional isolates. P. acnes was finally classified into nine isotypes, and isolates obtained from sarcoid and non-sarcoid tissue did not differ. Although the finding did not link P. acnes to sarcoidosis, the present study clarified the cell invasiveness of P. acnes and the close correlation of cell invasiveness to the serotype and genotype of the two invasion-associated P. acnes genes.
Furukawa
Asuka
A
Department of Human Pathology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan.
Uchida
Keisuke
K
Ishige
Yuki
Y
Ishige
Ikuo
I
Kobayashi
Intetsu
I
Takemura
Tamiko
T
Yokoyama
Tetsuji
T
Iwai
Kazuo
K
Watanabe
Kunitomo
K
Shimizu
Shinichiro
S
Ishida
Noriko
N
Suzuki
Yoshimi
Y
Suzuki
Takashige
T
Yamada
Tetsuo
T
Ito
Takashi
T
Eishi
Yoshinobu
Y
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000382:microbiology; D008074:Lipoproteins / Q000235:genetics; D008168:Lung / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology*; D051379:Mice; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011425:Propionibacterium acnes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D011817:Rabbits; D012507:Sarcoidosis / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012703:Serotyping
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008074:Lipoproteins; C116063:P60 protein, bacteria; C484918:trigger-factor protein, Propionibacterium acnes