PEG-PBLG nanoparticle-mediated HSV-TK/GCV gene therapy for oral squamous cell carcinoma.
OBJECTIVE
Previous studies revealed that drug-loaded poly(ethylene-glycol)-poly(gamma-benzyl-L-glutamate) (PEG-PBLG) nanoparticles exhibit favorable pharmacokinetic characteristics in the treatment of oral squamous cell carcinoma (OSCC). In this study, the characteristics and anticancer effect of herpes simplex virus thymidine kinase (HSV-TK)-loaded PEG-PBLG nanoparticles for OSCC were investigated.


METHODS
HSV-TK-loaded PEG-PBLG nanoparticles were prepared and their morphology, DNA protection and gene-transfer efficiency were evaluated. Their anticancer effect in vitro and in vivo was determined.


CONCLUSIONS
HSV-TK-loaded PEG-PBLG nanoparticles have a core-shell structure and DNA protection and higher gene-transfer efficiency. PEG-PBLG nanoparticle-mediated HSV-TK/ganciclovir (GCV) had a strong anticancer effect on Tca8113 cells in vitro and buccal carcinoma induced in golden hamsters.


CONCLUSIONS
PEG-PBLG nanoparticles may be a superior gene carrier in future clinical applications because of their DNA protection and higher gene-transfer efficiency. The HSV-TK/GCV suicide-gene system had significant antitumor effects on OSCC.
Yu
Dongsheng
D
Department of Oral & Maxillofacial Surgery, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, 510055, PR China.
Wang
Anxun
A
Huang
Hongzhang
H
Chen
Yiyang
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000628:therapy*; D003131:Combined Modality Therapy / Q000379:methods; D006224:Cricetinae; D015774:Ganciclovir / Q000627:therapeutic use; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000235:genetics / Q000628:therapy*; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D013937:Thymidine Kinase / Q000378:metabolism
D000970:Antineoplastic Agents; D000998:Antiviral Agents; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols; D013937:Thymidine Kinase; D015774:Ganciclovir