Endogenous microRNAs induced by heat-shock reduce myocardial infarction following ischemia-reperfusion in mice.
We investigated the role of microRNAs (miRNA) in protection against ischemia/reperfusion (I/R) injury in heart. Mice subjected to cytoprotective heat-shock (HS) showed a significant increase of miRNA-1, miRNA-21 and miRNA-24 in the heart. miRNAs isolated from HS mice and injected into non-HS mice significantly reduced infarct size after I/R injury, which was associated with the inhibition of pro-apoptotic genes and increase in anti-apoptotic genes. Chemically synthesized miRNA-21 also reduced infarct size, whereas a miRNA-21 inhibitor abolished this effect. Overall, these studies for the first time provide evidence for the potential role of endogenously synthesized miRNA's in cardioprotection following I/R injury.
Yin
Chang
C
Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, 1101 E. Marshall St. Sanger Hall, Box 980281, Richmond, VA 23298-0281, USA.
Wang
Xiaoyin
X
Kukreja
Rakesh C
RC
D000818:Animals; D018882:Heat Stress Disorders / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D035683:MicroRNAs / Q000138:chemical synthesis / Q000235:genetics* / Q000302:isolation & purification; D009203:Myocardial Infarction / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000150:complications*
C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; C517731:Mirn1 microRNA, mouse; C529080:Mirn24 microRNA, mouse