[Tracing to the source of staphylococcus aureus isolates from ice cream].
OBJECTIVE
To investigate the contamination of Staphylococcus aureus isolates in ice cream by phenotypic typing and molecular typing.


METHODS
The Staphylococcus aureus isolates were separated from ice cream, filler, cutter, salves and material. The separated isolates were characterized by drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E, G-J) genes and pulsed-field gel electrophoresis (PFGE) types.


RESULTS
Two Staphylococcus aureus isolates were separated, one from ice cream, another from cutter. Their characteristics of drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E,G-J) genes and PFGE type were the same.


CONCLUSIONS
The two Staphylococcus aureus isolates were the same clone. The contaminated Staphylococcus aureus isolates could be traced to the contaminated cutters.
Zhang
Yan-Jun
YJ
Zhejiang Province Center for Disease Control and Prevention, Hangzhou 310009, China. yjzhang@cdc.zj.cn
Xu
Dan-Ge
DG
Fang
Ye-Zhen
YZ
Gong
Pu
P
Zhu
Min
M
Bao
Fang-Zhen
FZ
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000235:genetics; D005516:Food Microbiology; D007054:Ice Cream / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D004768:Enterotoxins