[Influence of avian influenza virus NS1 protein on the expression of IP-10 in BEAS-2B cells].
OBJECTIVE
To investigate the influence of avian influenza virus (AIV) NS1 protein on the expression of interferon-inducible protein 10 (IP-10).


METHODS
NSI gene from virus A/Anhui/1/2005 (H5N1), NS1 gene inserted with 80-84 amino acids from virus A/Anhui/1/2005 (H5N1) and NS1 gene from virus A/Puerto Rico/8/1934 (H1N1) were cloned into the eukaryotic expression vector pEGFP-N1, and transfected into BEAS-2B cells, IP-10 expression level in transfected cells was detected by flow cytometry.


RESULTS
Compared with the control group pEGFP-N1, expression of these three different NS1 genes can down-regulate the expression of IP-10 in BEAS-2B cells, but there is no significant difference as to the lower level among them.


CONCLUSIONS
NS1 protein of A/Anhui/1/2005 (H5N1) can down-regulate the expression level of IP-10, but this may not clarify its relationship with the virulence of AIV.
Jia
Xiao-Jun
XJ
Department of Animal Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Zhou
Jian-Fang
JF
Wang
Jing-Yu
JY
Dong
Jie
J
Bo
Hong
H
Li
Zi
Z
Li
Kui-Biao
KB
Lan
Yu
Y
Shu
Yue-Long
YL
D002460:Cell Line; D054357:Chemokine CXCL10 / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000378:metabolism*; D007251:Influenza, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C085820:INS1 protein, influenza virus; D017361:Viral Nonstructural Proteins