title: Interest of ImmunoCAP system to recombinant omega-5 gliadin for the diagnosis of exercise-induced wheat allergy.
abstract: BACKGROUND
  omega-5 gliadin is a major allergen in exercise-induced wheat allergy (EIWA), but it is also implicated in immediate-type reactions to wheat. An ImmunoCAP assay to measure omega-5 gliadin-specific IgE has become available. This study aimed to evaluate this new biological test in wheat allergy diagnosis and to also determine if it was able to discriminate EIWA from other types of wheat allergy.
  
  
  METHODS
  Sixty-one patients with wheat allergy were divided into 3 groups as a function of their symptoms (EIWA, immediate-type reactions and atopic dermatitis). These patients underwent skin prick tests with purified omega gliadins and ImmunoCAP to wheat flour, gluten and recombinant omega-5 gliadin.
  
  
  RESULTS
  The experimental data showed that 78% of EIWA patients had a positive skin prick test to natural omega-5 gliadin and the same proportion had detectable specific IgE to recombinant omega-5 gliadin, indicating that omega-5 gliadin is the main allergen, but not the only one, in our population. Additionally, we showed that this detection was not EIWA specific since omega-5 gliadin-specific IgE was detected in 30% of other patients who had a wheat allergy. These results lead to a positive predictive value of 37.5% and to a negative predictive value of 91%.
  
  
  CONCLUSIONS
  Although not specific to EIWA, the new ImmunoCAP omega-5 gliadin is an important biological test because of its negative predictive value. In case of food-dependent exercise-induced allergy, the absence of omega-5 gliadin-specific IgE will almost completely exclude the implication of wheat.
authors.affiliation: Genclis SAS, Vandoeuvre-lès-Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Jacquenet
authors.forename: M
authors.initials: M
authors.lastname: Morisset
authors.forename: F
authors.initials: F
authors.lastname: Battais
authors.forename: S
authors.initials: S
authors.lastname: Denery-Papini
authors.forename: A
authors.initials: A
authors.lastname: Croizier
authors.forename: E
authors.initials: E
authors.lastname: Baudouin
authors.forename: B
authors.initials: B
authors.lastname: Bihain
authors.forename: D A
authors.initials: DA
authors.lastname: Moneret-Vautrin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology; D052179:Antigens, Plant; D002648:Child; D002675:Child, Preschool; D015444:Exercise; D005260:Female; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007073:Immunoglobulin E / Q000097:blood*; D007223:Infant; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D012882:Skin Tests; D021182:Wheat Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D011994:Recombinant Proteins; C475180:omega-5 gliadin, wheat; D007073:Immunoglobulin E; D005903:Gliadin