Modulated growth of nanoparticles. Application for sensing nerve gases.
Hydrolysis of acetylthiocholine mediated by acetylcholine esterase yields the thiol-bearing compound thiocholine. At trace concentrations, thiocholine modulates the growth of Au-Ag nanoparticles on seeding gold nanoparticles in the presence of ascorbic acid. Inhibition of the enzyme by 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide (BW284c51) or by diethyl p-nitrophenyl phosphate (paraoxon) produces lower yields of thiocholine, promoting the catalytic growth of Au-Ag nanoparticles. Here, we describe the development of a simple and sensitive colorimetric assay for the detection of AChE inhibitors.
Virel
Ana
A
CIC biomaGUNE, Parque Tecnologico de San Sebastian, Paseo Miramon 182, 20009 San Sebastian, Spain.
Saa
Laura
L
Pavlov
Valeri
V
D000110:Acetylcholinesterase / Q000737:chemistry; D000122:Acetylthiocholine / Q000737:chemistry; D001205:Ascorbic Acid / Q000737:chemistry; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000032:analysis / Q000737:chemistry; D002619:Chemical Warfare Agents / Q000032:analysis* / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006868:Hydrolysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D010261:Paraoxon / Q000032:analysis / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013860:Thiocholine / Q000032:analysis / Q000737:chemistry
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D012834:Silver; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; D000122:Acetylthiocholine; D013860:Thiocholine; D006046:Gold; D000110:Acetylcholinesterase; D001205:Ascorbic Acid; D010261:Paraoxon