title: Convenient TLC-based Identification Test for the Crude Drug "Pogostemoni Herba".
abstract: TLC and HPLC were used to identify possible chemical markers for evaluating the quality of the crude drug "Pogostemoni herba" (aerial part of Pogostemon cablin), which is a component of Kampo medicines. In addition to the reported patchouli alcohol and 2-hydroxy-6-methyl-3-(4-methylpentanoyl)-4-pyrone, three phenylethanoids were isolated from this plant material for the first time: acteoside, isoacteoside, and crenatoside. The usefulness of these compounds as indicators of the crude commercial drug under various TLC conditions was examined, and patchouli alcohol was found to give a definite spot with a reproducible Rf value. Therefore, we propose TLC of the methanol (MeOH) extract using patchouli alcohol as a marker as a convenient method for identifying the crude drug Pogostemoni herba.
authors.affiliation: College of Pharmaceutical Sciences, Matsuyama University, Matsuyama City, Japan. amakura@cc.matsuyama-u.ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Amakura
authors.forename: Morio
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Chika
authors.initials: C
authors.lastname: Mouri
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mikage
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kawahara
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D002109:Caffeic Acids / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D000432:Methanol; D010636:Phenols / Q000302:isolation & purification*; D012717:Sesquiterpenes / Q000302:isolation & purification*; D059808:Polyphenols
chemical_list: D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D000432:Methanol; D010636:Phenols; D012717:Sesquiterpenes; C083069:crenatoside; C058956:acteoside; D059808:Polyphenols; C064683:isoacteoside; C052983:patchouli alcohol