Chitosan nanofiber scaffold enhances hepatocyte adhesion and function.
To enhance cell attachment and promote liver functions of hepatocytes cultured in bioreactors, a chitosan nanofiber scaffold was designed and prepared via electrospinning. Effects of the scaffold on hepatocyte adhesion, viability and function were then investigated. Data showed that hepatocytes on chitosan nanofiber scaffold exhibited better viability and tighter cell-substrate contact than cells on regular chitosan film. In addition, urea synthesis, albumin secretion and cytochrome P450 activity of hepatocytes on chitosan nanofiber scaffold were all 1.5 to 2 folds higher than the controls. Glycogen synthesis was also increased as compared with the controls. These results suggested the potential application of this chitosan nanofiber scaffold as a suitable substratum for hepatocyte culturing in bioreactors.
Chu
Xue-Hui
XH
Department of Hepatobiliary Surgery, Drum Tower Affiliated Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, People's Republic of China.
Shi
Xiao-Lei
XL
Feng
Zhang-Qi
ZQ
Gu
Zhong-Ze
ZZ
Ding
Yi-Tao
YT
D019149:Bioreactors; D002448:Cell Adhesion / Q000187:drug effects*; D018929:Cell Culture Techniques; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis; D022781:Hepatocytes / Q000502:physiology*; D049329:Nanostructures / Q000737:chemistry*; D012709:Serum Albumin / Q000378:metabolism; D014508:Urea / Q000378:metabolism
D012709:Serum Albumin; D014508:Urea; D006003:Glycogen; D048271:Chitosan; D003577:Cytochrome P-450 Enzyme System