title: Cutaneous lymphocyte antigen-positive T cells may predict the development of acute GVHD: alterations and differences of CLA+ T- and NK-cell fractions.
abstract: Acute GVHD (aGVHD) is a serious complication after allogeneic SCT (allo-SCT). However, an adequate immunological index is not yet available for assessing its severity. We analyzed the fraction of cutaneous lymphocyte antigen (CLA)+ cells in peripheral blood T and natural killer (NK) cells in 33 patients and evaluated its association with aGVHD. The CLA+ T-cell fraction often increased 3-7 days before the onset of aGVHD, and the maximum percentage of CLA+ T cells in grades II-IV aGVHD cases was significantly higher than that in grade 0 or I aGVHD (P<0.01). When the cutoff value of the maximum CLA+ T-cell percentage was set at 20%, any higher percentage was a significant risk for the development of severe aGVHD (P<0.0001). The maximum CLA+ T-cell percentage was significantly correlated with a high body temperature, low percutaneous oxygen saturation, and fibrinogen/fibrin degradation product D-dimer level. The post-allo-SCT CLA+ T cells exhibited a high ability to produce IL-2 and IFN-gamma, and may be the effectors and immunological markers for aGVHD. The CLA+ NK-cell-fraction steadily increased 2-4 weeks after allo-SCT but was not influenced by aGVHD. The CLA+ T-cell percentage may predict the development of severe aGVHD in clinical settings.
authors.affiliation: Department of Internal Medicine, Division of Hematology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan. j-tutti@nifty.com
authors.forename: J
authors.initials: J
authors.lastname: Tsuchiyama
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: I
authors.initials: I
authors.lastname: Fuse
authors.forename: Y
authors.initials: Y
authors.lastname: Aizawa
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D033581:Stem Cell Transplantation; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines