High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.
Iyer
Gopal
G
Department of Chemistry & Biochemistry, David Geffen School of Medicine, UCLA, Los Angeles, California 90024, USA. gopal@chem.ucla.edu
Michalet
Xavier
X
Chang
Yun-Pei
YP
Pinaud
Fabien F
FF
Matyas
Stephanie E
SE
Payne
Gregory
G
Weiss
Shimon
S
D006241:Haptens / Q000737:chemistry*; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D045663:Quantum Dots
D006241:Haptens; D007128:Immunoglobulin Fragments; D011506:Proteins