p53 gene mutational rate, Gleason score, and BK virus infection in prostate adenocarcinoma: Is there a correlation?
Prostate cancer represents the second leading cause of cancer deaths in Western countries. Viral infections could play a role in prostate carcinogenesis. Human polyomavirus BK (BKV) is a possible candidate because of its transforming properties. In this study, BKV sequences in urine, blood, fresh, and paraffin-embedded prostate cancer samples from 26 patients were searched using Q-PCR analysis. T antigen (TAg) and p53 localization in neoplastic cells were evaluated by immunohistochemical analysis. Also, the presence of mutations in 5-9 exons of p53 gene was analyzed. Results showed that BKV-DNA was found in urine (54%), plasma (31%), and in fresh prostate cancer specimens (85%). The analysis of p53 gene evidenced several mutations in high Gleason patients, according to tumor advanced stage. Immunohistochemical analysis results evidenced the localization of p53 and TAg into cytoplasm, whereas in TAg-negative tumors, p53 was nuclear. This study suggests that BKV acts as cofactor in the pathogenesis of prostate cancer. These observations emphasize previous studies regarding the cellular pathways that may be deregulated by BKV.
Russo
Giuseppe
G
Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania.
Anzivino
Elena
E
Fioriti
Daniela
D
Mischitelli
Monica
M
Bellizzi
Anna
A
Giordano
Antonio
A
Autran-Gomez
Anamaria
A
Di Monaco
Franco
F
Di Silverio
Franco
F
Sale
Patrizio
P
Di Prospero
Laura
L
Pietropaolo
Valeria
V
D000230:Adenocarcinoma / Q000821:virology*; D000957:Antigens, Viral, Tumor / Q000032:analysis; D001739:BK Virus / Q000302:isolation & purification*; D001769:Blood / Q000821:virology; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D027601:Polyomavirus Infections / Q000150:complications*; D011467:Prostate / Q000737:chemistry / Q000821:virology; D011471:Prostatic Neoplasms / Q000821:virology*; D012720:Severity of Illness Index; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D014412:Tumor Virus Infections / Q000150:complications*; D014556:Urine / Q000821:virology
D000957:Antigens, Viral, Tumor; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53