title: Parvovirus B19 profiles in patients presenting with acute myocarditis and chronic dilated cardiomyopathy.
abstract: BACKGROUND
  Parvovirus B19 (B19V) is the most prevalent cardiotropic virus in endomyocardial biopsies (EMBs) from patients presenting with acute myocarditis (AMC) and chronic dilated cardiomyopathy (DCM). We elucidated the role of B19V specific IgG and IgM antibodies against native VP2-capsids and denatured VP1-, VP2- and NS1-proteins, which discriminate disease acuity in other B19V related diseases, in patients presenting with clinically suspected AMC and DCM for the determination of the viral infection stage.
  
  
  METHODS
  n=62 prospectively enrolled AMC (n=33) and DCM (n=29) patients were investigated. B19V genomes were amplified in EMBs by nested PCR (nPCR). B19V-specific IgG and IgM were investigated by recomLine blots in the sera.
  
  
  RESULTS
  B19V genomes were detectable by nPCR with comparable frequencies in AMC (63.6%) and DCM patients (51.7%), respectively. IgM antibodies were detectable in 18.1% of the AMC, but not in DCM patients. In patients with myocardial B19V infection, antibody profiles indicating recent infections were more frequent in AMC (47.6%) compared with DCM patients (6.7%), while persistent/reactivating profiles were significantly more prevalent in DCM (20.0%) compared with AMC (0%) patients (p<0.05).
  
  
  CONCLUSIONS
  IgM B19V antibodies can be detected primarily in AMC, but not in DCM patients. The discrimination of the B19V specific IgG antibodies using recomLine blots may be suitable to distinguish acute versus chronic, and persistent/reactivating infection status in patients with myocardial B19V infection.
authors.affiliation: Department of Cardiology and Pneumonology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Felicitas
authors.initials: F
authors.lastname: Escher
authors.forename: Susanne
authors.initials: S
authors.lastname: Modrow
authors.forename: Titus
authors.initials: T
authors.lastname: Sabi
authors.forename: Uwe
authors.initials: U
authors.lastname: Kühl
authors.forename: Dirk
authors.initials: D
authors.lastname: Lassner
authors.forename: Heinz-Peter
authors.initials: HP
authors.lastname: Schultheiss
authors.forename: Michel
authors.initials: M
authors.lastname: Noutsias
mesh_terms: D000208:Acute Disease; D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D002311:Cardiomyopathy, Dilated / Q000821:virology*; D016022:Case-Control Studies; D002908:Chronic Disease; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009205:Myocarditis / Q000821:virology*; D010322:Parvoviridae Infections / Q000150:complications / Q000821:virology*; D016732:Parvovirus B19, Human / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral