Toxicity of polybrominated diphenyl ethers (DE-71) in chicken (Gallus gallus), mallard (Anas platyrhynchos), and American kestrel (Falco sparverius) embryos and hatchlings.
Embryonic survival, pipping and hatching success, and sublethal biochemical, endocrine, and histological endpoints were examined in hatchling chickens (Gallus gallus), mallards (Anas platyrhynchos), and American kestrels (Falco sparverius) following air cell administration of a pentabrominated diphenyl ether (penta-BDE; DE-71) mixture (0.01-20 microg/g egg) or polychlorinated biphenyl (PCB) congener 126 (3,3',4,4',5-pentachlorobiphenyl; 0.002 microg/g egg). The penta-BDE decreased pipping and hatching success at concentrations of 10 and 20 microg/g egg in kestrels but had no effect on survival endpoints in chickens or mallards. Sublethal effects in hatchling chickens included ethoxyresorufin-O-dealkylase (EROD) induction and histological changes in the bursa, but these responses were not observed in other species. Polychlorinated biphenyl congener 126 (positive control) reduced survival endpoints in chicken and kestrel embryos and caused sublethal effects (EROD induction, reduced bursal mass and follicle size) in chickens. Mallards were clearly less sensitive than the other species to administered penta-BDE and PCB 126. In a second experiment, the absorption of penta-BDE (11.1 microg/g egg, air cell administered during early development) into the contents of chicken and kestrel eggs was determined at various intervals (24 h postinjection, midincubation, and pipping). By pipping, 29% of the penta-BDE administered dose was present in the egg contents in chickens, and 18% of the administered dose was present in kestrel egg contents. Based on uptake in kestrels, the lowest-observed-effect level on pipping and hatching success may be as low as 1.8 microg total penta-BDE/g egg, which approaches concentrations detected in eggs of free-ranging birds. Because some penta-BDE congeners are still increasing in the environment, the toxic effects observed in the present study are cause for concern in wildlife.
McKernan
Moira A
MA
Marine, Estuarine, and Environmental Sciences and Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA.
Rattner
Barnett A
BA
Hale
Robert C
RC
Ottinger
Mary Ann
MA
D000818:Animals; D001846:Bone Development / Q000187:drug effects; D002642:Chick Embryo / Q000187:drug effects*; D002645:Chickens; D004372:Ducks / Q000196:embryology / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity; D046093:Falconiformes / Q000196:embryology / Q000254:growth & development; D005411:Flame Retardants / Q000633:toxicity*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008221:Lymphoid Tissue / Q000187:drug effects / Q000254:growth & development; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism
D004785:Environmental Pollutants; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C086401:pentabromodiphenyl ether