Hypericum grandifolium Choisy: a species native to Macaronesian Region with antidepressant effect.
OBJECTIVE
Various species of Hypericum genus have been used in the Canary Islands as sedative, diuretic, vermifuge, wound healing, antihysteric and antidepressant agent. Studies have shown that methanol extract of Hypericum grandifolium Choisy is active in tetrabenazine-induced ptosis and forced swimming tests. In the current study, the aqueous, butanol and chloroform fractions obtained from the methanol extract as well as three sub-fractions derived from the chloroform fraction were evaluated for their central nervous effects in mice, particularly their antidepressant activity.


METHODS
The central nervous effect of different fractions and sub-fractions of Hypericum grandifolium was evaluated in mice using various behavioural models including locomotor and muscle relaxant activity, forced swimming test, effect on normal body temperature, barbiturate-induced sleep, tetrabenazine-induced syndrome and 5-hydroxytryptohan-induced head twitches and syndrome.


RESULTS
We found that the butanol and chloroform fractions and all sub-fractions showed an antidepressant effect in the forced swimming test, the chloroform fraction being the most active. They produced no effects or only a slight depression of locomotor activity. Chloroform fraction significantly increased the pentobarbital-induced sleeping time, produced a slight but significant hypothermia and antagonized tetrabenazine-induced ptosis, whereas the butanol fraction produced a slight potentiation of 5-HTP-induced head twitches and syndrome.


CONCLUSIONS
The present results, together with previous pharmacological and phytochemical data, indicated that Hypericum grandifolium possess antidepressant-like effects in mice and that different constituents, such as the flavonoids and the benzophenone derivatives, could be responsible at least in part for the antidepressant effects observed for this species.
Sánchez-Mateo
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CC
Departamento de Farmacología, Facultad de Farmacia, Universidad de La Laguna, c/Astrofísico Francisco Sánchez s/n, 38071 La Laguna, Tenerife, Spain. csanchez@ull.es
Bonkanka
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CX
Rabanal
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RM
D000818:Animals; D000928:Antidepressive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D003863:Depression / Q000188:drug therapy*; D004195:Disease Models, Animal; D020902:Hypericum / Q000737:chemistry*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D013030:Spain; D013550:Swimming
D000928:Antidepressive Agents; D010936:Plant Extracts