title: Analysis of molecular determinants of PRL-3.
abstract: In order to analyse whether a C-terminal polybasic sequence represents a nuclear localization signal (NLS) we obtained several truncated and mutant forms of protein of regerating liver (PRL)-3 and evaluated their subcellular localization as compared to the wild-type form. Our results invalidate the hypothesis that this is an NLS. We also analysed the influence of the C- and N-terminal residues on the phosphatase activity of PRL-3. Our results provide in vitro evidence that the C-terminal CAAX motif, besides directing the protein farnesylation, plays an additional regulatory role by inhibiting the catalytic efficiency of PRL-3. Taking into account the results we obtained, as well as reported data, we propose a hypothetical molecular mechanism for the nucleocytoplasmic localization and transfer of PRL-3.
authors.affiliation: Department of Enzymology, Institute of Biochemistry, Bucharest, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Pascaru
authors.forename: Carmen
authors.initials: C
authors.lastname: Tanase
authors.forename: Andrei M
authors.initials: AM
authors.lastname: Vacaru
authors.forename: Patricia
authors.initials: P
authors.lastname: Boeti
authors.forename: Elena
authors.initials: E
authors.lastname: Neagu
authors.forename: Irinel
authors.initials: I
authors.lastname: Popescu
authors.forename: Stefan E
authors.initials: SE
authors.lastname: Szedlacsek
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000473:pathology; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism; D012038:Regeneration
chemical_list: D009363:Neoplasm Proteins; D010744:Phosphoric Monoester Hydrolases; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases