title: [Effect of silver-hydroxyapatite/titania nanocomposites on proliferation, vitality and ultrastructure of osteoblasts].
abstract: OBJECTIVE
  Osteoblasts behaviors were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg-HA/TiO2) in vitro.
  
  
  METHODS
  Rat calvaria osteoblasts were cultured in the presence of 10 mirog/mL-500 mirog/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope (TEM).
  
  
  RESULTS
  Both cell proliferation and vitality were restricted by addition of nanoparticles, while the respiration rates as an index for unit cell viability were enhanced. nAg-HA/TiO2 at low concentration showed superiority. No remarkable ultrastructural changes were observed in the osteoblasts exposed to nanoparticles. The differences in cell proliferation and cell vitality between nAg-HA/TiO2 group and nHA group at the same concentration were insignificant.
  
  
  CONCLUSIONS
  nAg-HA/TiO2 restricted cells proliferation, but triggered unit cell viability. Cytocompatibility of nAg-HA/TiO2 is almost the same as that of nHA's.
authors.affiliation: Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Jing-chao
authors.initials: JC
authors.lastname: Zhang
authors.forename: An-chun
authors.initials: AC
authors.lastname: Mo
authors.forename: Hong-kun
authors.initials: HK
authors.lastname: Wu
authors.forename: Juan
authors.initials: J
authors.lastname: Liao
authors.forename: Yu-bao
authors.initials: YB
authors.lastname: Li
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry; D004622:Embryo, Mammalian; D053761:Nanocomposites; D010006:Osteoblasts / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
chemical_list: C009495:titanium dioxide; D012834:Silver; D017886:Durapatite; D014025:Titanium