title: Recurrent chromosomal abnormalities in human embryonic stem cells.
abstract: Cultured human embryonic stem (hES) cells have a known predisposition to aneuploidy of chromosomes 12, 17 and X. We studied 17 hES cell lines by array-based comparative genomic hybridization (aCGH) and found that the cells accumulate other recurrent chromosomal abnormalities, including amplification at 20q11.21 and a derivative chromosome 18. These genomic changes have a variable impact at the transcriptional level.
authors.affiliation: Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium. laudia.spits@uzbrussel.be
authors.forename: Claudia
authors.initials: C
authors.lastname: Spits
authors.forename: Ileana
authors.initials: I
authors.lastname: Mateizel
authors.forename: Mieke
authors.initials: M
authors.lastname: Geens
authors.forename: Afroditi
authors.initials: A
authors.lastname: Mertzanidou
authors.forename: Catherine
authors.initials: C
authors.lastname: Staessen
authors.forename: Yves
authors.initials: Y
authors.lastname: Vandeskelde
authors.forename: Josiane
authors.initials: J
authors.lastname: Van der Elst
authors.forename: Inge
authors.initials: I
authors.lastname: Liebaers
authors.forename: Karen
authors.initials: K
authors.lastname: Sermon
mesh_terms: D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D053595:Embryonic Stem Cells / Q000166:cytology / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data