title: [Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish].
abstract: OBJECTIVE
  To study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish.
  
  
  METHODS
  In situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time.
  
  
  RESULTS
  Nspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head.
  
  
  CONCLUSIONS
  Nspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Gong
authors.forename: Bo-Qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Jian-Gang
authors.initials: JG
authors.lastname: Yuan
authors.forename: An-Ming
authors.initials: AM
authors.lastname: Meng
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000254:growth & development / Q000378:metabolism; D063150:Polycomb Repressive Complex 1; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D012097:Repressor Proteins; D029961:Zebrafish Proteins; C549851:pcgf1 protein, Danio rerio; D063150:Polycomb Repressive Complex 1