title: Corneal innervation in mesocephalic and brachycephalic dogs and cats: assessment using in vivo confocal microscopy.
abstract: OBJECTIVE
  To determine the density of the canine and feline corneal neural network in healthy dogs and cats using in vivo confocal microscopy (IVCM). ANIMALS EXAMINED: A total of 16 adult dogs (9 Mesocephalic breeds, 7 Brachycephalic breeds) and 15 cats (9 Domestic Short-haired cats (DSH), 6 Persian cats) underwent IVCM.
  
  
  METHODS
  Animals were examined with a confocal corneal microscope (HRTII/RCM; Heidelberg Retina Tomograph II/Rostock Cornea Module, Heidelberg Engineering, Dossenheim, Germany). The investigations focused on the distribution of the corneal nerves and quantification of central subepithelial and subbasal nerve plexus.
  
  
  RESULTS
  The corneal stromal nerve trunks, subepithelial and subbasal nerve plexus were observed. The nerve fiber density (NFD) quantified in nerve fiber length in mesocephalic dogs were 12.39 +/- 5.25 mm/mm(2) in the subepithelial nerve plexus and 14.87 +/- 3.08 mm/mm(2) in the subbasal nerve plexus. The NFD of the subepithelial nerve plexus in DSH cats was 15.49 +/- 2.7 and 18.4 +/- 3.84 mm/mm(2) in the subbasal nerve plexus. The subbasal NFD of DSH cats was significantly higher than in mesocephalic dogs (P = 0.037). The subepithelial NFD in brachycephalic dogs, and Persian cats were 10.34 +/- 4.71 and 9.50 +/- 2.3 mm/mm(2), respectively. The subbasal NFD measured 11.80 +/- 3.73 mm/mm(2) in brachycephalic dogs, and 12.28 +/- 4.3 mm/mm(2) NFD in Persian cats, respectively. The subepithelial and subbasal NFD in Persian cats were significantly lower than in DSH cats (P = 0.028, respectively, P = 0.031), in contrast to brachycephalic vs. mesocephalic dogs.
  
  
  CONCLUSIONS
  The noninvasive IVCM accurately detects corneal innervation and provides a reliable quantification of central corneal nerves.
authors.affiliation: Institute of Veterinary Anatomy, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany. chka@arcor.de
authors.forename: Christiane
authors.initials: C
authors.lastname: Kafarnik
authors.forename: Jens
authors.initials: J
authors.lastname: Fritsche
authors.forename: Sven
authors.initials: S
authors.lastname: Reese
mesh_terms: D000818:Animals; D001947:Breeding; D002415:Cats / Q000033:anatomy & histology*; D003315:Cornea / Q000033:anatomy & histology* / Q000294:innervation*; D004285:Dogs / Q000033:anatomy & histology*; D005260:Female; D008297:Male; D018613:Microscopy, Confocal / Q000662:veterinary*; D012016:Reference Values; D013045:Species Specificity