On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles.
As a step toward a fully automated front-end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI-MS, using a replaceable on-chip digestion microcolumn based on self-assembled magnetic particles.
Le Nel
Anne
A
Laboratoire PCC, Institut Curie, Paris, France.
Krenkova
Jana
J
Kleparnik
Karel
K
Smadja
Claire
C
Taverna
Myriam
M
Viovy
Jean-Louis
JL
Foret
Frantisek
F
D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D014357:Trypsin / Q000737:chemistry*
D004129:Dimethylpolysiloxanes; D011506:Proteins; C013830:baysilon; D014357:Trypsin