Upregulation of SOX-2, FZD9, Nestin, OCT-4 and FGF-4 expression in human chorion derived-stem cells after angiogenic induction.
Angiogenic induction was made to promote angiogenesis by differentiating stem cells towards endothelial cells. However, the stemness property of induced cells has not been revealed yet. Hence, we aim to evaluate the differential mRNA expression of stemness genes in human chorion-derived stem cells (CDSC) after being cultured in EDM50 comprised bFGF and VEGF. Results indicated that CDSC cultured in EMD50 expressed significantly higher mRNA level of Sox-2, FZD9, BST-1 and Nestin. In addition Oct-4, FGF-4 and ABCG-2 were also upregulated. Our finding suggested that CDSC after angiogenic induction enhanced its stem cell properties. This could be contributed for the mechanism of stem cell therapy in ischemic problem.
Abdul Rahman
H
H
Department of Pathology, Universiti Kebangsaan Malaysia.
Manzor
N F Mohd
NF
Tan
G C
GC
Tan
A E
AE
Chua
K H
KH
D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology*; D005260:Female; D051519:Fibroblast Growth Factor 4 / Q000235:genetics*; D051157:Frizzled Receptors / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D007381:Intermediate Filament Proteins / Q000235:genetics*; D018919:Neovascularization, Physiologic; D009419:Nerve Tissue Proteins / Q000235:genetics*; D064231:Nestin; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D010865:Pilot Projects; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D055748:SOXB1 Transcription Factors / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology*; D015854:Up-Regulation / Q000235:genetics*
C496445:FGF4 protein, human; C105574:FZD3 protein, human; D051519:Fibroblast Growth Factor 4; D051157:Frizzled Receptors; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D050814:Octamer Transcription Factor-3; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D055748:SOXB1 Transcription Factors