title: Investigating the translocation of lambda-DNA molecules through PDMS nanopores.
abstract: We investigate the translocation of lambda-DNA molecules through resistive-pulse polydimethylsiloxane (PDMS) nanopore sensors. Single molecules of lambda-DNA were detected as a transient current increase due to the effect of DNA charge on ionic current through the pore. DNA translocation was found to deviate from a Poisson process when the interval between translocations was comparable to the duration of translocation events, suggesting that translocation was impeded during the presence of another translocating molecule in the nanopore. Characterization of translocation at different voltage biases revealed that a critical voltage was necessary to drive DNA molecules through the nanopore. Above this critical voltage, frequency of translocation events was directly proportional to DNA concentration and voltage bias, suggesting that transport of DNA from the solution to the nanopore was the rate limiting step. These observations are consistent with experimental results on transport of DNA through nanopores and nanoslits and the theory of hydrodynamically driven polymer flow in pores.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Yi-Heng
authors.initials: YH
authors.lastname: Sen
authors.forename: Rohit
authors.initials: R
authors.lastname: Karnik
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000032:analysis* / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D016062:Porosity; D011489:Protein Denaturation; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D012710:Serum Albumin, Bovine; D004247:DNA