Rapid DNA multi-analyte immunoassay on a magneto-resistance biosensor.
We present the rapid and sensitive detection of amplified DNA on a giant magneto-resistance sensor using superparamagnetic particles as a detection label. The one-step assay is performed on an integrated and miniaturized detection platform suitable for application into point-of-care devices. A double-tagged PCR amplification product of the LamB gene of the Escherichia coli bacterium was used to investigate binding kinetics of the assay. We applied magnetic actuation to concentrate the target-particle complexes at the sensor surface and to remove unbound particles from the sensor surface. We achieved biological dose-response curves detecting 4-250pM amplicon concentrations in a one-step format in total assay times of less than 3min. Using various tag-antibody combinations specific for one of the individual genes, multi-analyte detection is shown of several antibiotic resistance genes of the food pathogen Salmonella.
Koets
M
M
Philips Research, High Tech Campus 11, 5656 AE Eindhoven, The Netherlands.
van der Wijk
T
T
van Eemeren
J T W M
JT
van Amerongen
A
A
Prins
M W J
MW
D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008280:Magnetics / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004247:DNA