[The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 (H5N1)].
OBJECTIVE
To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1.


METHODS
The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology. The candidate genes were selected and confirmed by quantitative real-time PCR.


RESULTS
The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well.


CONCLUSIONS
Our results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.
Hu
Xiao-Fen
XF
State Key Laboratory for Infectious Diseases, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing 100052, China.
Liu
Qin-Zhi
QZ
Li
Chuan
C
Dong
Jie
J
Zhou
Jian-Fang
JF
Wang
Min
M
Shu
Yue-Long
YL
Liu
Hong-Tu
HT
Liang
Mi-Fang
MF
Li
De-Xin
DX
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D053124:Influenza A Virus, H5N1 Subtype / Q000502:physiology*; D007251:Influenza, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis