title: Development of an immunoglobulin M capture-based enzyme-linked immunosorbent assay for diagnosis of acute infections with Bartonella henselae.
abstract: We describe the development of an immunoglobulin M-specific enzyme-linked immunosorbent assay for the detection of an early antibody response to Bartonella henselae, the causative agent of cat scratch disease, bacillary angiomatosis, and endocarditis. This assay discriminates between B. henselae-positive and -negative patient samples with sensitivity and specificity values of 100% and 97.1%, respectively.
authors.affiliation: Antigen Discovery Division, Research and Development Department, Medical Diagnostic Laboratories, Hamilton, New Jersey 08690-3303, USA. jhoey@mdlab.com
authors.forename: John G
authors.initials: JG
authors.lastname: Hoey
authors.forename: Fernando
authors.initials: F
authors.lastname: Valois-Cruz
authors.forename: Hannah
authors.initials: H
authors.lastname: Goldenberg
authors.forename: Yekaterina
authors.initials: Y
authors.lastname: Voskoboynik
authors.forename: Jenna
authors.initials: J
authors.lastname: Pfiffner
authors.forename: Richard C
authors.initials: RC
authors.lastname: Tilton
authors.forename: Eli
authors.initials: E
authors.lastname: Mordechai
authors.forename: Martin E
authors.initials: ME
authors.lastname: Adelson
mesh_terms: D016917:Angiomatosis, Bacillary / Q000175:diagnosis / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000097:blood*; D018416:Bartonella henselae / Q000276:immunology / Q000302:isolation & purification*; D002372:Cat-Scratch Disease / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M