Mud-puddling in the yellow-spined bamboo locust, Ceracris kiangsu (Oedipodidae: Orthoptera): does it detect and prefer salts or nitrogenous compounds from human urine?
C. kiangsu adults were observed visiting human urine, especially on hot summer days. The main chemicals in fresh human urine include inorganic salts and CO(NH(2))(2.) When human urine was incubated, NH(4)HCO(3) became the richest nitrogenous compound. The phagostimulants, repellents and attractants in urine were identified here. On the filter papers treated with fresh or incubated urine samples, the 5th instar nymphs and the adults started and continued gnawing around the edges, in contrast to the 3rd and the 4th instar nymphs. The consumed areas were dramatically greater on the filters treated with the urine samples incubated for 3-6 days. The feedings of both male and female adults were also stimulated by several urine-borne components such as NaCl, NaH(2)PO(4), Na(2)SO(4), KCl, NH(4)Cl and NH(4)HCO(3) but not by CO(NH(2))(2). Among them NaCl was the most powerful phagostimulant. The repelling, or attractive/arresting effects of CO(NH(2))(2) and NH(4)HCO(3) were also evaluated by a two-choice test. When exposed to water- and CO(NH(2))(2) solution-immersed filters simultaneously, the adults prefer to stay on water-immersed filter. In contrast, when provided water- and NH(4)HCO(3) solution-treated filters, the adults prefer to stay on NH(4)HCO(3) solution-treated filter. This demonstrated that CO(NH(2))(2) acted as a repellent and NH(4)HCO(3) as an attractant/arrestant. In the bamboo forest, similar feeding behavior was also elicited by NaCl, NH(4)HCO(3) but not by CO(NH(2))(2). Comparing to NaCl solution, a mixed solution of NaCl and CO(NH(2))(2) (1:1) significantly decreased the consumed area of the treated filters whereas a mixed solution of NaCl and NH(4)HCO(3) (1:1) dramatically increased the consumed area. These results demonstrated that the phagostimulatory effect by NaCl was reduced by CO(NH(2))(2) in fresh urine and was enhanced by NH(4)HCO(3) in incubated urine.
Shen
Ke
K
Key Laboratory of Monitoring and Management of Plant Diseases and Pests Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
Wang
Hao-Jie
HJ
Shao
Lin
L
Xiao
Kai
K
Shu
Jin-Ping
JP
Xu
Tian-Sen
TS
Li
Guo-Qing
GQ
D000818:Animals; D001639:Bicarbonates / Q000032:analysis / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects*; D005260:Female; D006110:Grasshoppers / Q000187:drug effects*; D006801:Humans; D008297:Male; D012737:Sex Factors; D012965:Sodium Chloride / Q000032:analysis / Q000494:pharmacology*; D014508:Urea / Q000032:analysis / Q000494:pharmacology*; D014556:Urine / Q000737:chemistry
D001639:Bicarbonates; D012965:Sodium Chloride; C027043:ammonium bicarbonate; D014508:Urea