Comparison of SYBR Green I and TaqMan real-time PCR formats for the analysis of her2 gene dose in human breast tumors.
We compared two technologies of real-time PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for quantification of the dose of her2 gene in breast tumors. The maximum increase in the gene dose in TaqMan and SYBR Green I analyses was 10- and 5-fold, respectively. In was found that TaqMan and SYBR Green I technologies allow detection of the matrix in amounts corresponding to 1-100 and 2.5-40.0 ng genomic DNA, respectively. Tenfold increase in the gene dose leads to incorrect evaluation of multiplication ratio in the SYBR Green I analysis. These results suggest that TaqMan technology is more preferable for correct evaluation of her2 gene dose.
Matsenko
N U
NU
Laboratory of Gene Engineering Methods, Institute of Molecular and Biophysics, Siberian Division of the Russian Academy of Medical Sciences. n.matsenko@gmail.com
Rijikova
V S
VS
Kovalenko
S P
SP
D052160:Benzothiazoles; D001943:Breast Neoplasms / Q000235:genetics*; D003959:Diamines; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism*; D018628:Gene Dosage; D006801:Humans; D009930:Organic Chemicals / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D011804:Quinolines; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; D018719:Erb-b2 Receptor Tyrosine Kinases; C098022:SYBR Green I; C508053:ERBB2 protein, human