title: A fluorescence sensor for detection of geranyl pyrophosphate by the chemo-ensemble method.
abstract: Hexaamide receptor 4 and coumarin phosphate 5 form an ensemble for effective sensing of geranyl pyrophosphate (GPP). Fluorescence resonance energy transfer in the 4 + 5 ensemble diminishes when 5 is replaced by GPP. Receptor 4 binds selectively with GPP over other anions, including fatty acids. The 4-GPP complex in 1:1 stoichiometry is inferred by NMR and fluorescence titrations. Receptor 4 contains a pseudotetrahedral cleft to accommodate GPP via multiple hydrogen bonds, and the two aliphatic chains exert additional hydrophobic interactions.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Kuan-Hung
authors.initials: KH
authors.lastname: Chen
authors.forename: Jen-Hai
authors.initials: JH
authors.lastname: Liao
authors.forename: Hsin-Yu
authors.initials: HY
authors.lastname: Chan
authors.forename: Jim-Min
authors.initials: JM
authors.lastname: Fang
mesh_terms: D000577:Amides / Q000737:chemistry; D002138:Calibration; D003374:Coumarins / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011106:Polyisoprenyl Phosphates / Q000032:analysis*; D017186:Titrimetry
chemical_list: D000577:Amides; D003374:Coumarins; D005456:Fluorescent Dyes; D011106:Polyisoprenyl Phosphates; C015234:geranyl pyrophosphate; C030123:coumarin