Candida ogatae sp. nov., an anamorphic member of the Kuraishia clade.
Three methanol-assimilating yeast strains representing a hitherto undescribed species were isolated from rotten wood and freshwater samples collected in Hungary. Analysis of the D1/D2 large subunit rRNA gene sequences placed the strains in the Kuraishia clade; however, no ascospore formation was observed. These strains differ from Candida hungarica, the genetically most closely related recognized species, by four and five substitutions in D1/D2 and by >1% and 4% differences in the internal transcribed spacer and in the mitochondrial small subunit rRNA gene regions, respectively. Some phenotypic differences were also observed. Candida ogatae, a novel yeast species, is proposed to accommodate these isolates. The type culture is NCAIM Y.01845(T) (CBS 10924, NRRL Y-48474).
Péter
Gábor
G
National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Sciences, Corvinus University of Budapest, Budapest, Hungary. gabor.peter@uni-corvinus.hu
Tornai-Lehoczki
Judit
J
Dlauchy
Dénes
D
D002175:Candida / Q000145:classification* / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D006814:Hungary; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000166:cytology; D014934:Wood / Q000382:microbiology*
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S