[Diagnostic significance of immunophenotyping and detection of gene rearrangement in subcutaneous panniculitis-like T-cell lymphoma].
OBJECTIVE
To explore the diagnostic implication of immunophenotyping and gene rearrangement in subcutaneous panniculitis-like T-cell lymphoma (SPTL).


METHODS
According to the selection criteria of 2005 WHO-EORTC classification for cutaneous lymphomas, 20 SPTL patients were enrolled in this study. A 10-antibody panel was used for immunophenotyping and in addition, polymerase chain reaction for TCR gamma and IgH gene rearrangement and in situ hybridization for EBER1/2 were also employed.


RESULTS
There were 9 males and 11 female with a mean age of 29.5 years. Immunophenotypic study showed that all the patients expressed one to three T-cell associated antigens (CD2, CD3 or CD45RO), 18 patients were positive for beta F1, 18 for CD8, 20 for TIA-1 and 16 for granzyme B. None of the patients expressed CD4, CD20 and CD56. TCR gamma gene rearrangement was found in 16 of 20 cases (80.0%) and none for IgH gene rearrangement. The positive rate of EBER1/2 was 25.0% (5/20).


CONCLUSIONS
Since the majority of SPTL patients show clonal TCR gene rearrangements, correlations among clinical presentation, histological features, immunophenotype and gene rearrangement data are considered important in confirming a diagnosis of SPTL.
Wan
Chuan
C
Department of Dermatovenereology, West China Hospital of Sichuan University, Chengdu 610041, China.
Xu
Chen
C
Wang
Lin
L
Li
Gan-di
GD
Jiang
Wei
W
Zhang
Wen-yan
WY
Tang
Yuan
Y
Kan
Bei
B
Liu
Wei-ping
WP
D000293:Adolescent; D000328:Adult; D018951:Antigens, CD20 / Q000276:immunology; D019002:CD56 Antigen / Q000032:analysis* / Q000276:immunology; D002648:Child; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D017403:In Situ Hybridization; D016399:Lymphoma, T-Cell / Q000145:classification* / Q000175:diagnosis / Q000235:genetics / Q000276:immunology; D016410:Lymphoma, T-Cell, Cutaneous / Q000145:classification / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015434:Panniculitis; D012367:RNA, Viral / Q000276:immunology / Q000302:isolation & purification; D012878:Skin Neoplasms / Q000276:immunology; D040521:Subcutaneous Tissue; D055815:Young Adult
D018951:Antigens, CD20; D019002:CD56 Antigen; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral