Time-dependent activation of ERK1/2 in nerve terminals of the dentin-pulp complex following bradykinin treatment.
The extracellular signal-regulated kinases 1 and 2 (ERK1/2) have been implicated in the inflammation-dependent sensitization of nociceptors, and the inflammatory mediator bradykinin (BK) led to a reduced threshold in the nociceptor terminals, activating intracellular signaling by phosphorylating receptors and ion channels. The effects of BK on the non-transcriptional modulation of the ERK1/2 in the peripheral nociceptor terminals, including in nerve endings of the dentin-pulp complex, are unknown. The time-dependent effects of BK (10(-7) M) on the ERK1/2 phosphorylation in nerve terminals of the dentin-pulp complex were investigated by quantitative and double immunolabeling with organ bath experiments. In nerve terminals, total and p-ERK1/2 were detected. In comparison with the controls, the numbers of p-ERK1/2-positive nerve endings increased after 1 and 3 min and decreased after 10 min of BK treatment. Analysis of the data indicates that BK induces phosphorylation-mediated local activation of ERK1/2 in nerve terminals modulating nociception in the dentin-pulp complex.
Korkmaz
Y
Y
Department of Operative and Preventive Dentistry and Endodontics, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, Germany. yueksel.korkmaz@uni-duesseldorf.de
Bloch
W
W
Schneider
K
K
Zimmer
S
S
Addicks
K
K
Raab
W H-M
WH
D000818:Animals; D000911:Antibodies, Monoclonal; D001920:Bradykinin / Q000494:pharmacology*; D003782:Dental Pulp / Q000294:innervation*; D003804:Dentin / Q000294:innervation*; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D009411:Nerve Endings / Q000201:enzymology* / Q000473:pathology; D009412:Nerve Fibers / Q000187:drug effects / Q000473:pathology; D009619:Nociceptors / Q000187:drug effects / Q000473:pathology; D009804:Odontoblasts / Q000201:enzymology / Q000473:pathology; D010146:Pain / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D043222:Ubiquitin Thiolesterase / Q000187:drug effects
D000911:Antibodies, Monoclonal; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse; D001920:Bradykinin