Stability and antibacterial activity of bacteriocins produced by Bacillus thuringiensis and Bacillus thuringiensis ssp. kurstaki.
Bacteriocins are antimicrobial peptides that are produced by bacteria and toxic to bacterial strains closely related to the producer strain. It has previously been reported that Bacillus thuringiensis strain NEB17 and Bacillus thuringiensis subsp. kurstaki BUPM4 produce the bacteriocins thuricin 17 (3,162 Da) and bacthuricin F4 (3,160.05 Da), respectively. Here, we demonstrate that these bacteriocins have functional similarities and show a similar spectrum of antimicrobial activities against indicator strains. We also studied the effects of sterilization methods on the recovery and biological activities of these bacteriocins. They were completely degraded by autoclaving and the two were similarly affected by the tested filter membranes. Polyvinylidene fluoride (PVDF), polyestersulfone (PES), and cellulose acetate (CA) are suitable for filter sterilization of these bacteriocins. The two bacteriocins were stable across a range of storage conditions. These data will facilitate their utilization in food preservation or agricultural applications.
Jung
Woo-Jin
WJ
Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University,Gwangju 500-757, Korea.
Mabood
Fazli
F
Souleimanov
Alfred
A
Zhou
Xiaomin
X
Jaoua
Samir
S
Kamoun
Fakher
F
Smith
Donald L
DL
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000145:classification / Q000187:drug effects; D001409:Bacillus cereus / Q000187:drug effects; D001410:Bacillus megaterium / Q000187:drug effects; D001413:Bacillus thuringiensis / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D001430:Bacteriocins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004355:Drug Stability; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D013696:Temperature
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C059740:thuricin