Investigation of matrix metalloproteinase-1--1607 1G/2G polymorphism in a Turkish population with periodontitis.
OBJECTIVE
Matrix metalloproteinase-1 (MMP-1) is a proteolytic enzyme that degrades extracellular matrix and plays a fundamental role during destruction of periodontal tissues. The aim of this study was to examine the association between MMP-1 -1607 1G/2G polymorphism and chronic periodontitis susceptibility in a Turkish population.


METHODS
A total of 180 subjects were enrolled in this study. All the subjects received a periodontal examination including full-mouth clinical attachment loss measurements, probing depths, plaque index scores, gingival index scores and radiographic bone loss ratios. Three groups formed according to periodontal conditions were healthy, moderate periodontitis and severe periodontitis groups. MMP-1 -1607 1G/2G gene promoter polymorphism was genotyped using a polymerase chain reaction-restriction fragment length polymorphism method.


RESULTS
Analysis of the polymorphism showed no differences in distribution of the MMP-1 -1607 1G/2G polymorphism among healthy, moderate periodontitis and severe periodontitis groups (p>0.05). When the groups were further stratified by smoking status, we found no significant differences in genotype distributions, allele frequencies and carriage rates among any groups either (p>0.05).


CONCLUSIONS
On the basis of the results, no significant association is found for the MMP-1 -1607 1G/2G polymorphism with susceptibility to periodontitis. Moreover, smoking status did not seem to affect this result.
Ustun
Kemal
K
Oral and Dental Health Center, Selçuklu, Konya, Turkey. k_ustun@yahoo.com
Alptekin
Nilgün Ozlem
NO
Hakki
Sema S
SS
Hakki
Erdogan E
EE
D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000201:enzymology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012907:Smoking; D014421:Turkey
D020781:Matrix Metalloproteinase 1