title: Cyclooxygenase-2 polymorphisms confer susceptibility to sarcoidosis but are not related to prognosis.
abstract: BACKGROUND
  The aim of this multicenter study was to investigate the relationship between single nucleotide polymorphisms (SNPs) of the cyclooxygenase-2 (COX2) gene and susceptibility to sarcoidosis, as well as the relation between these SNPs and the evolution of the disease.
  
  
  METHODS
  This multicenter investigation involved seven hospitals in Spain. We used a case-control design followed by a prospective follow-up study. Sarcoid patients were recruited from the participating institutions during outpatient routine visits. Age- and gender-matched control subjects were recruited mainly from among outpatients attending the participating hospitals. Four SNPs in the COX2 gene (COX2.5909 T > G, COX2.8473 T > C, COX2.926 G > C, and COX2.3050 G > C) were genotyped using fluorescent hybridization probes among 131 patients with sarcoidosis (63 males; mean age: 47 +/- 15 years) and 157 healthy controls (83 males; mean age: 50 +/- 16 years). We employed a binomial multiple logistic regression analysis to test the association between the selected SNPs and disease susceptibility. The clinical, functional and radiological prognosis of the sarcoidosis patients was determined after a mean follow-up of 37.4 +/- 30.4 months.
  
  
  RESULTS
  Carriers of the homozygous CC genotype of the COX2.8473 T > C polymorphism had a higher risk of sarcoidosis compared with TT carriers (OR: 3.08; 95% CI: 1.2-7.7; p = 0.035). 84% of patients achieved improvement or complete remission at follow-up. No association between the investigated SNPs and prognosis was seen.
  
  
  CONCLUSIONS
  Our data suggest that the homozygous CC genotype of the COX2.8473 T > C polymorphism may be associated with sarcoidosis susceptibility. No significant association with prognosis was detected.
authors.affiliation: Hospital Universitario Virgen del Rocío, Seville, Spain. lcampos@separ.es
authors.forename: José Luis
authors.initials: JL
authors.lastname: Lopez-Campos
authors.forename: David
authors.initials: D
authors.lastname: Rodriguez-Rodriguez
authors.forename: Eulogio
authors.initials: E
authors.lastname: Rodriguez-Becerra
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Alfageme Michavila
authors.forename: Jose Fernandez
authors.initials: JF
authors.lastname: Guerra
authors.forename: Francisco Javier García
authors.initials: FJ
authors.lastname: Hernandez
authors.forename: Alvaro
authors.initials: A
authors.lastname: Casanova
authors.forename: Javier
authors.initials: J
authors.lastname: Fernández de Córdoba Gamero
authors.forename: Ana
authors.initials: A
authors.lastname: Romero-Ortiz
authors.forename: Elena
authors.initials: E
authors.lastname: Arellano-Orden
authors.forename: Ana
authors.initials: A
authors.lastname: Montes-Worboys
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D051546:Cyclooxygenase 2 / Q000235:genetics*; D017931:DNA Primers; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008168:Lung / Q000000981:diagnostic imaging; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies; D011859:Radiography; D017565:Sarcoidosis, Pulmonary / Q000000981:diagnostic imaging / Q000235:genetics*
chemical_list: D017931:DNA Primers; D051546:Cyclooxygenase 2