title: [The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 (H5N1)].
abstract: OBJECTIVE
  To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1.
  
  
  METHODS
  The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology. The candidate genes were selected and confirmed by quantitative real-time PCR.
  
  
  RESULTS
  The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well.
  
  
  CONCLUSIONS
  Our results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.
authors.affiliation: State Key Laboratory for Infectious Diseases, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing 100052, China.
authors.forename: Xiao-Fen
authors.initials: XF
authors.lastname: Hu
authors.forename: Qin-Zhi
authors.initials: QZ
authors.lastname: Liu
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Shu
authors.forename: Hong-Tu
authors.initials: HT
authors.lastname: Liu
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Liang
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D053124:Influenza A Virus, H5N1 Subtype / Q000502:physiology*; D007251:Influenza, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis