title: Inhibition of SNARE-driven neuroexocytosis by plant extracts.
abstract: Neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) proteins mediate membrane fusion between synaptic vesicle and presynaptic membrane, resulting in neurotransmitter release. SNARE proteins are specific substrates of botulinum neurotoxins (BoNT) which are now widely used for therapeutic and cosmetic purposes. While BoNT blocks neuroexocytosis by cleaving SNAREs, inhibiting SNARE assembly process might exert the same effect on neurotransmission. In the present study, some extracts of 100 plants reduced neurotransmitter release by inhibiting SNARE complex formation in neuronal cells. The extracts effectively paralyzed muscle of rat phrenic nerve-hemidiaphragm preparation. Our results raise the possibility that SNARE folding inhibitors from natural resources might replace some special BoNT application fields.
authors.affiliation: School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, 440-746, South Korea.
authors.forename: Chang Hwa
authors.initials: CH
authors.lastname: Jung
authors.forename: Yoo Soo
authors.initials: YS
authors.lastname: Yang
authors.forename: Jun-Seob
authors.initials: JS
authors.lastname: Kim
authors.forename: Yeon-Kyun
authors.initials: YK
authors.lastname: Shin
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Hwang
authors.forename: Eui Dong
authors.initials: ED
authors.lastname: Son
authors.forename: Hong Hwa
authors.initials: HH
authors.lastname: Lee
authors.forename: Koo Min
authors.initials: KM
authors.lastname: Chung
authors.forename: Jung Mi
authors.initials: JM
authors.lastname: Oh
authors.forename: Jong Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kweon
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000187:drug effects; D005089:Exocytosis / Q000187:drug effects*; D009466:Neuromuscular Blocking Agents / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D010243:Paralysis; D010791:Phrenic Nerve / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D050600:SNARE Proteins / Q000037:antagonists & inhibitors*; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D009466:Neuromuscular Blocking Agents; D010936:Plant Extracts; D050600:SNARE Proteins