title: The structure of the efflux pump AcrB in complex with bile acid.
abstract: Gastrointestinal bacteria, like Escherichia coli, must remove bile acid to survive in the gut. Bile acid removal in E. coli is thought to be mediated primarily by the multidrug efflux pump, AcrB. Here, we present the structure of E. coli AcrB in complex with deoxycholate at 3.85 A resolution. All evidence suggests that bile acid is transported out of the cell via the periplasmic vestibule of the AcrAB-TolC complex.
authors.affiliation: Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London, UK.
authors.forename: David
authors.initials: D
authors.lastname: Drew
authors.forename: Mirjam M
authors.initials: MM
authors.lastname: Klepsch
authors.forename: Simon
authors.initials: S
authors.lastname: Newstead
authors.forename: Ralf
authors.initials: R
authors.lastname: Flaig
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: De Gier
authors.forename: So
authors.initials: S
authors.lastname: Iwata
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Beis
mesh_terms: D018360:Crystallography, X-Ray; D003840:Deoxycholic Acid / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry* / Q000378:metabolism; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D003840:Deoxycholic Acid; D029968:Escherichia coli Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D050505:Mutant Proteins; C083496:AcrB protein, E coli