Blood-spinal cord barrier permeability in experimental spinal cord injury: dynamic contrast-enhanced MRI.
After a primary traumatic injury, spinal cord tissue undergoes a series of pathobiological changes, including compromised blood-spinal cord barrier (BSCB) integrity. These vascular changes occur over both time and space. In an experimental model of spinal cord injury (SCI), longitudinal dynamic contrast-enhanced MRI (DCE-MRI) studies were performed up to 56 days after SCI to quantify spatial and temporal changes in the BSCB permeability in tissue that did not show any visible enhancement on the post-contrast MRI (non-enhancing tissue). DCE-MRI data were analyzed using a two-compartment pharmacokinetic model. These studies demonstrate gradual restoration of BSCB with post-SCI time. However, on the basis of DCE-MRI, and confirmed by immunohistochemistry, the BSCB remained compromised even at 56 days after SCI. In addition, open-field locomotion was evaluated using the 21-point Basso-Beattie-Bresnahan scale. A significant correlation between decreased BSCB permeability and improved locomotor recovery was observed.
Cohen
David M
DM
Department of Diagnostic and Interventional Imaging, University of Texas Medical School at Houston, Houston, TX 77030, USA.
Patel
Chirag B
CB
Ahobila-Vajjula
Pallavi
P
Sundberg
Laura M
LM
Chacko
Tessy
T
Liu
Shi-Jie
SJ
Narayana
Ponnada A
PA
D000418:Albumins; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000473:pathology; D003287:Contrast Media; D005119:Extravasation of Diagnostic and Therapeutic Materials; D005682:Gadolinium / Q000008:administration & dosage / Q000494:pharmacology; D008279:Magnetic Resonance Imaging; D008297:Male; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis / Q000473:pathology*; D013997:Time Factors
D000418:Albumins; D003287:Contrast Media; D012965:Sodium Chloride; D005682:Gadolinium