Serological survey of domestic animals for tick-borne encephalitis and Bhanja viruses in northeastern Hungary.
Blood sera collected from 400 domestic animals (260 cattle, 100 Merino sheep, and 40 Hutzul horses) in northeastern Hungary in 2005 were examined for antibodies against two tick-borne viruses, tick-borne encephalitis flavivirus (TBEV) and Bhanja bunyavirus (BHAV). Using ELISA as screening test and plaque-reduction neutralization as confirmatory test, seropositivity to TBEV was found to be 26.5% in cattle, 7.0% in sheep, and 0.0% in horses. Among cattle, the animals up to 3 years old had significantly lower seroprevalence rate than those in older age groups. Natural foci of tick-borne encephalitis are obviously present in northeastern Hungary. On the other hand, no antibodies neutralizing BHAV were detected in the domestic animals.
Sikutová
Silvie
S
Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Medical Zoology Laboratory, Kvetná 8, Brno 60365, Czech Republic. sikutova@ivb.cz
Hornok
Sándor
S
Hubálek
Zdenek
Z
Dolezálková
Iva
I
Juricová
Zina
Z
Rudolf
Ivo
I
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004669:Encephalitis Viruses, Tick-Borne / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000453:epidemiology* / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005843:Geography; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D006814:Hungary / Q000453:epidemiology; D009500:Neutralization Tests; D016851:Orthobunyavirus / Q000302:isolation & purification*; D012703:Serotyping; D012757:Sheep Diseases / Q000453:epidemiology / Q000821:virology*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral