[The dynamic changing profiles of peripheral white blood cell telomere length in populations of different ages living at different altitude areas].
OBJECTIVE
To investigate the relationship between the length of telomere DNA and age at different altitude areas.


METHODS
All 172 peripheral blood samples were randomly selected from healthy individuals of different ages from 25 to 65 years old. High altitude group (47 males, 48 females) living at an altitude of 4380 m (HA group), sea level group (39 males, 38 females) living at an altitude of 43 m (SL group). The terminal restriction fragment (TRF) length of telomere DNA was measured by Southern blotting analysis. The plasma levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were assayed.


RESULTS
Average TRF lengths of males and females in HA groups were 10.45 +/- 1.35 and 10.50 +/- 1.45. Average TRF lengths of males and females in SL groups were 11.29 +/- 1.10 and 11.31 +/- 1.13. A negative correlation was shown between the average TRF length and age of males in two groups (P < 0.01). This was also the case for females. ANOVA test was used to analyze the difference between TRF length and gender at different ages (P < 0.001). It was shown that there was significant difference in TRF length between the male (25 years old and 55 years old) and female (25 years old and 55 years old) in two groups at different ages (P < 0.05). The plasma levels of SOD and MDA were significant different between HA groups and SL groups (25-44 years old groups/45-65 years old groups) (P < 0.05).


CONCLUSIONS
Obviously shortening of telomere was observed by increasing of ages in high altitude groups. There was a negative correlation between the length of telomere DNA and ages. Telomere shortening became more obviously in high altitude group than in sea level group in keeping with the age increases.
Zhong
Ying-Cheng
YC
High Altitude Medical Research Center, Qinghai University Medical College, Xining 810001, China.
Luo
Peng-Li
PL
Jin
Guo-En
GE
Yang
Hui-Lian
HL
Han
Shu-Fen
SF
Ge
Ri-Li
RL
D000328:Adult; D000367:Age Factors; D000368:Aged; D000531:Altitude; D001773:Blood Cells; D004247:DNA / Q000235:genetics*; D005260:Female; D006801:Humans; D007962:Leukocytes; D008297:Male; D008315:Malondialdehyde; D008875:Middle Aged; D012091:Repetitive Sequences, Nucleic Acid; D013482:Superoxide Dismutase; D016615:Telomere / Q000235:genetics*
D008315:Malondialdehyde; D004247:DNA; D013482:Superoxide Dismutase