[Yeasts isolated from the patients of the Department of Dentistry, University Hospital in Hradec Králové in the period of 1996-2007].
OBJECTIVE
The goal was to evaluate the spectrum of yeasts isolated from biological materials from patients treated at the Department of Dentistry, Faculty of Medicine and University Hospital in Hradec Kralove in the years 1996 to 2007. The study focused on the relationships between yeasts and some clinical and epidemiological characteristics.


METHODS
The study involved patients with the positive mycological findings attending the Department of Dentistry in the period. Electronic records kept at the Department of Clinical Microbiology were processed and then analyzed statistically by regression analysis and chi2 test.


RESULTS
During the period 1996 to 2007, 530 patients (26.9 %) showed culture-positive yeasts in a total of 713 biological specimens (23.9 %). Mycological findings were more common (chi2 test, p = 0.00003) in women (n = 348; 30.4 %) than in men (n = 182; 22.0 %). The mucous membrane of the mouth and the tongue were most often colonized by yeasts (51.5 %). Candida albicans (93.2 %) predominated among the yeasts isolated. The overall proportion of non-albicans Candida species (C. tropicalis, C. glabrata and C. krusei) was relatively low (24.7 %) and there was no significant trend of changes during the period in the spectrum of yeasts. However, a comparison of the distribution of yeast over the period showed a statistically higher incidence of C. tropicalis in females than in males (13.2 % vs. 6.6 %; chi2 test, p = 0.020) and more frequent positivity of C. krusei in men compared to women (4.9 % vs. 1.1 %; chi2 test, p = 0.007). Majority of yeast isolates was associated with the diagnosis of mucosal infection in the mouth cavity (89.0 %); smaller part represented diseases of teeth and adjacent anatomical sites (periodontium) and uncommon infections (angular cheilitis, salivary gland, Sjögren syndrome).


CONCLUSIONS
The study showed that yeasts were commonly present in the oral cavity either as normal microflora or cause of fungal infections, especially in women. The tongue, the palate and buccal mucosa were most frequent anatomical location with yeasts isolated. Candida albicans remains predominant species, but C. tropicalis and C. glabrata can frequently be isolated. There were no trends in the change of yeast spectrum during that period.
Vejsová
Marcela
M
Department of Clinical Microbiology, University Hospital Hradec Králové and Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové Czech Republic.
Buchta
Vladimír
V
Kudiyirickal
George Marina
GM
Krivcíková
Lucie
L
Slezák
Radovan
R
D002175:Candida / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification*