Gamma-ray up-regulated holocarboxylase synthetase gene.
OBJECTIVE
To investigate the effects of holocarboxylase synthetase (HCS) gene to irradiation in a time- and dose-dependent manner.


METHODS
AHH-1 cells, Hela cells, and the nude mice inoculated with tumor cells were exposed to gamma-ray of cobalt 60. The mRNA level of HCS was detected using real-time PCR. The profiles of mRNA of HCS after radiation were analyzed and described.


RESULTS
The expression of HCS gene was higher in AHH-1 cells than that in Hela cells. Furthermore, both AHH-1 and Hela displayed similar time-dependent transcriptional levels of HCS gene to radiations at the dose of 2 and 10 Gy. We set the parameters of D, V, R, F, N to quantitatively analyze HCS gene regulation in response to irradiation. We also observed that irradiation resulted in higher levels of HCS in human hepatocyte xenografts, compared with three other types of human tumor xenografts. 2 and 4 Gy radiation had little influence on the HCS gene of human lung cancer and brain cancer, mammary gland cancer was more sensitive to the 4 Gy gamma-ray dose compared with the 2 Gy gamma-ray dose.


CONCLUSIONS
HCS is a good radiation-responsive gene. It may be used as a candidate for developing novel biomarkers of radiation damage and it has a great potential to be used in radiation-therapy of liver tumors.
Ding
Kuke
K
Biomedical Engineering School, Capital Medical University, Beijing, 100069, China.
Yang
Chunjie
C
Shen
Jingjing
J
Xu
Lili
L
Li
Yanling
Y
Zhou
Pinkun
P
Zeng
Yanjun
Y
D000818:Animals; D019731:Carbon-Nitrogen Ligases / Q000235:genetics* / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation / Q000528:radiation effects*
D012333:RNA, Messenger; D019731:Carbon-Nitrogen Ligases; C023312:holocarboxylase synthetases