title: Dynamic contrast-enhanced magnetic resonance imaging for estimating tumor proliferation and microvessel density of oral squamous cell carcinomas.
abstract: We evaluated the relationship between histopathological prognostic factors, tumor proliferation microvessel density (MVD), and enhancement parameters in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in oral squamous cell carcinoma (SCC). Twenty-eight T2 and T3 patients with primary oral SCC underwent DCE-MRI using three-dimensional fast imaging with a steady-state precession sequence. Tumor cell proliferation and MVD of all surgical specimens were evaluated using immunohistochemical staining with CD34 and the antibody for proliferating cell nuclear antigen (PCNA). Regression analysis was used to statistically analyze the relationship between the PCNA labeling index or MVD and each of three DCE-MRI parameters: maximum CI (CI-max), maximum CI gain (CI-gain) and the CI-gain / CI-max ratio). The PCNA labeling index and MVD showed significant correlations with the CI-gain/CI-max ratio (P=0.0012, r=0.581 and P=0.00141, r=0.574, respectively). The assessment of DCE-MRI parameters may prove to be a valuable non-invasive method for assessing tumor cell proliferation and MVD of patients with oral cancer.
authors.affiliation: Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 5-1, Shikata-cho, 2-chome, Okayama 700-8552, Japan.
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Unetsubo
authors.forename: Hironobu
authors.initials: H
authors.lastname: Konouchi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Yanagi
authors.forename: Jun
authors.initials: J
authors.lastname: Murakami
authors.forename: Masae
authors.initials: M
authors.lastname: Fujii
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: Miki
authors.initials: M
authors.lastname: Hisatomi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Asaumi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000473:pathology*; D003287:Contrast Media; D005260:Female; D019786:Gadolinium DTPA; D006801:Humans; D021621:Imaging, Three-Dimensional; D007564:Japan; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D055806:Microvessels / Q000473:pathology; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000098:blood supply / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D003287:Contrast Media; D018809:Proliferating Cell Nuclear Antigen; D019786:Gadolinium DTPA