title: Lipids of the ultra-thin square halophilic archaeon Haloquadratum walsbyi.
abstract: The lipid composition of the extremely halophilic archaeon Haloquadratum walsbyi was investigated by thin-layer chromatography and electrospray ionization-mass spectrometry. The analysis of neutral lipids showed the presence of vitamin MK-8, squalene, carotene, bacterioruberin and several retinal isomers. The major polar lipids were phosphatidylglycerophosphate methyl ester, phosphatidylglycerosulfate, phosphatidylglycerol and sulfated diglycosyl diether lipid. Among cardiolipins, the tetra-phytanyl or dimeric phospholipids, only traces of bisphosphatidylglycerol were detected. When the cells were exposed to hypotonic medium, no changes in the membrane lipid composition occurred. Distinguishing it from other extreme halophiles of the Halobacteriaceae family, the osmotic stress did not induce the neo-synthesis of cardiolipins in H. walsbyi. The difference may depend on the three-laminar structure of the cell wall, which differs significantly from that of other Haloarchaea.
authors.affiliation: Department of Medical Biochemistry, Biology and Physics, University of Bari, Bari, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Lobasso
authors.forename: Patrizia
authors.initials: P
authors.lastname: Lopalco
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mascolo
authors.forename: Angela
authors.initials: A
authors.lastname: Corcelli
mesh_terms: D002308:Cardiolipins / Q000032:analysis; D002855:Chromatography, Thin Layer; D006216:Halobacteriaceae / Q000737:chemistry* / Q000145:classification*; D016931:Halobacteriales / Q000145:classification; D008563:Membrane Lipids / Q000032:analysis* / Q000737:chemistry; D009997:Osmotic Pressure; D010743:Phospholipids / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002308:Cardiolipins; D008563:Membrane Lipids; D010743:Phospholipids