Short term effects of doxycycline on matrix metalloproteinases 2 and 9.
OBJECTIVE
To investigate the short term effects of Doxycycline on MMP-2 and MMP-9.


METHODS
Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells.


RESULTS
In plasma, MMP-9 activity was reduced (-22%, 95% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28%, 95% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52%, 95% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively).


CONCLUSIONS
Doxy decreases MMP-9 plasma levels by around 20%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.
Fiotti
Nicola
N
S C Clinica Medica Generale e Terapia Medica, Dipartimento di Scienze Cliniche, Morfologiche e Tecnologiche, Università degli Studi di Trieste, Trieste, Italy. fiotti@units.it
Altamura
Nicola
N
Moretti
Michèle
M
Wassermann
Stella
S
Zacchigna
Serena
S
Farra
Rossella
R
Dapas
Barbara
B
Consoloni
Lara
L
Giacca
Mauro
M
Grassi
Gabriele
G
Giansante
Carlo
C
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D004318:Doxycycline / Q000494:pharmacology*; D004656:Enalapril / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D009566:Nitrates / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D009566:Nitrates; D004656:Enalapril; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D004318:Doxycycline; D001241:Aspirin