Isolation and expression analysis of Alzheimer's disease-related gene xb51 in zebrafish.
XB51 protein is known to interact with the amino-terminal of the X11L protein and to be involved in Abeta40 generation, a hallmark of Alzheimer's disease. In this study, we isolated a zebrafish xb51 homologue and analyzed its spatio-temporal expression pattern during early brain development. The xb51 transcript was first detected in the forebrain at 22 hr post-fertilization. Expression of xb51 in the brain persisted by 36 hpf and became more complex in the brain after 48 hpf. The detailed expression domain of xb51 in the dorsal telencephalon was defined by several molecular markers: emx1, dlx2, lim1, islet1, neurod4/zath3, ngn1, her4, and elavl3/huC. The location of xb51-expressing cells was restricted in a subset of cells positive for elavl3/huC and acetylated alpha-tubulin, markers of differentiating and/or differentiated neurons. Together, these results suggest that xb51 may be required for maturation and maintenance of xb51-expressing neurons in the forebrain.
Kim
Hyun-Taek
HT
Department of Biology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon, South Korea.
Kim
Eun-Hye
EH
Yoo
Kyeong-Won
KW
Lee
Mi-Sun
MS
Choi
Jung-Hwa
JH
Park
Hae-Chul
HC
Yeo
Sang-Yeob
SY
Lee
Dong-Seok
DS
Kim
Cheol-Hee
CH
D000544:Alzheimer Disease; D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D016548:Prosencephalon / Q000196:embryology / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013687:Telencephalon / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D029961:Zebrafish Proteins; C534580:necab1 protein, zebrafish