title: Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue.
abstract: Accumulating evidence suggests that the delivery of human adipose tissue-derived stromal cells (hASCs) has great potential as regenerative therapy. This was performed to develop a method for expanding hASCs by reducing the amount of serum required. We demonstrate that hASCs were able to expand efficiently in media containing 2% serum and fibroblast growth factor-2. These cells, or low serum cultured hASCs (hLASCs), expressed cell surface markers similar to those on bone marrow-derived mesenchymal stem cells, and could be differentiated into cells of mesenchymal lineage. Of interest, hLASCs secreted higher levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) than hASCs cultured in 20% serum (hHASCs). Moreover, hLASC-conditioned media significantly increased endothelial cell (EC) proliferation and decreased EC apoptosis compared to that obtained from hHASCs or control media only. Antibodies against VEGF and HGF virtually negated these effects. When hASCs were administered into the ischemic hindlimbs of nude rats, hLASCs improved blood flow, increased capillary density, and raised the levels of VEGF and HGF in the muscles as compared with hHASCs. In conclusion, we demonstrate a novel low serum culture system for hASCs, which may have great potential in regenerative cell therapy for damaged organs in the clinical setting.
authors.affiliation: Department of Nephrology, Nagoya University Graduate School of Medicine, Showaku, Nagoya, Aichi, Japan.
authors.forename: Shigejiro
authors.initials: S
authors.lastname: Iwashima
authors.forename: Takenori
authors.initials: T
authors.lastname: Ozaki
authors.forename: Shoichi
authors.initials: S
authors.lastname: Maruyama
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Saka
authors.forename: Masato
authors.initials: M
authors.lastname: Kobori
authors.forename: Kaoru
authors.initials: K
authors.lastname: Omae
authors.forename: Hirotake
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Niimi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Toriyama
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kamei
authors.forename: Shuhei
authors.initials: S
authors.lastname: Torii
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Murohara
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yuzawa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Seiichi
authors.initials: S
authors.lastname: Matsuo
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D048430:Cell Shape; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D054377:Chemokine CXCL12 / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006614:Hindlimb / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000378:metabolism / Q000628:therapy; D017078:Laser-Doppler Flowmetry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D011923:Rats, Nude; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D050151:Subcutaneous Fat / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D015415:Biomarkers; D054377:Chemokine CXCL12; D017077:Culture Media, Conditioned; D016207:Cytokines; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor