[Evaluation of two immunochromatography kits for rapid diagnosis of rotavirus infections].
A prospective study was conducted to evaluate two immunochromatography (ICG) commercial kits for diagnosis of rotavirus infection, VIKIA Rota-Adeno (bioMérieux) and Simple Rota-Adeno (Operon). Reverse transcriptase and polymerase chain reaction (RT-PCR) with specific primers for the VP7 gene of group A rotavirus was used as the reference method. The sensitivity and specificity of the ICG tests compared with those of the reference method were 98.4% and 84.8%, respectively, for Simple Rota-Adeno (Operon), and 100% and 24.2% for VlKIA Rota-Adeno (bioMérieux). It is remarkable the low specificity of the latter method, which yields a high number of false positive results. The predictive value of a positive result by this method was only 71.6%. Most of the detected rotavirus strains corresponded to genotype G9P[8] (65%), followed by G1P[8] (25.4%) and G2P[8] (3.2%).
Téllez
C J
CJ
Departamento de Microbiología, Facultad de Medicina, Universidad de Valencia, Hospital Clinico Universitario de Valencia, Avda. Blasco Ibáñez, 17, 46010 Valencia, España.
Montava
R
R
Ribes
Juan M
JM
Tirado
M D
MD
Buesa
J
J
D002845:Chromatography; D006801:Humans; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013997:Time Factors
D011933:Reagent Kits, Diagnostic