Isolation techniques of murine bone marrow progenitor cells and their adipogenic, neurogenic and osteogenic differentiation capacity.
Bone marrow derived progenitor cells have been widely studied for its multipotent property and have proofed to be an important resource in regenerative medicine. However, the propagation of murine bone marrow appeared to be a great challenge as compared to other mammalian species. In this study, various isolation techniques and the plasticity of the isolated cells were evaluated. Our result shows that magnetic sorting technique yielded the most viable cells and displayed wider differentiation capacity.
Ng
A M H
AM
Tissue Engineering Laboratory, Universiti Kebangsaan Malaysia Medical Centre Kuala Lumpur.
Kojima
K
K
Kodoma
S
S
Ruszymah
B H I
BH
Aminuddin
B S
BS
Vacanti
A C
AC
D050156:Adipogenesis / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D010865:Pilot Projects; D023822:Tissue Engineering