title: Characterization of VP7 and VP4 genes of rotavirus strains: 1990-1994 and 2000-2002.
abstract: A total of 90 rotavirus-positive faecal specimens collected from patients hospitalized with diarrhoea in 1990-1994 (n=77) and 2000-2002 (n=13) were investigated for VP7 and VP4 genotypes. The specimens included 21 typable and 69 non-reactive or multireactive rotavirus strains as monitored by monoclonal antibody-based serotyping ELISA. Genotyping was carried out by multiplex PCR/sequencing using primers specific for both VP7 and VP4 genes. The contribution of common genotypes (G1P[8], G2P[4], G3P[8], G4P[8]) in causing rotavirus diarrhoea was 79.2% and 92.3% in the years 1990-1994 and 2000-2002, respectively, while G9P[8] infections were detected at lower levels (1.3% and 7.7%) at both time-points. There was a predominance of G1P[8] in 1990-1994 and of G2P[4] in 2000-2002. The detection of unusual rotavirus strains (G1P[6], G1P[4], G1P[19], G2P[8], G3P[4], G4P[6]) in 19.5% of the patients indicated a significant contribution of reassortants in causing diarrhoea in western India.
authors.affiliation: National Institute of Virology, Pune, Maharashtra, India.
authors.forename: J K
authors.initials: JK
authors.lastname: Zade
authors.forename: P
authors.initials: P
authors.lastname: Chhabra
authors.forename: S D
authors.initials: SD
authors.lastname: Chitambar
mesh_terms: D019943:Amino Acid Substitution; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology; D014644:Genetic Variation; D006801:Humans; D007194:India / Q000453:epidemiology; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D013997:Time Factors
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus