[CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV-infected individuals].
OBJECTIVE
To study the CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV infected individuals, and reveal the relationship between the CNAR and CD4+ cell count.


METHODS
CD8+ cells from HIV-1 sero-positive individuals were separated by immunomagnetic beads and mixed with CD4+ cells at different CD8 CD4 cell input ratios (2:1, 1:1, 0.5:1 and 0.25:1). Reverse transcriptase (RT) activity of cocultured supernatant was tested and compared with negative control and the suppression rate of HIV-1 replication was measured.


RESULTS
The average CD8:CD4 cell input ratios to reach 80% suppression of HIV replication in the group with CD4 < 300/microl and CD4 > 300/microl were 2.4:1 and 1.3:1, respectively (P < 0.05).


CONCLUSIONS
CNAR activity in HIV infected individuals is associated with CD4+ cell count. The ability to suppress HIV replication in subjects with CD4 > 300 is stronger than those with CD4 < 300.
Yuan
Lin
L
Center for Prevention and Control of Sexually Transmitted Diseases and AIDS, China Center for Disease Prevention and Control, Beijing 100050, China.
Ma
Li-Ying
LY
Zang
Xi-Hui
XH
Meng
Xiang-Dong
XD
Peng
Hong
H
Zhao
Quan-Bi
QB
Xing
Hui
H
Shao
Yi-Ming
YM
D000328:Adult; D000369:Aged, 80 and over; D019380:Anti-HIV Agents / Q000627:therapeutic use; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003428:Cross Infection / Q000188:drug therapy / Q000276:immunology*; D005260:Female; D006678:HIV / Q000187:drug effects / Q000276:immunology*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged
D019380:Anti-HIV Agents