title: In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system.
abstract: We demonstrate the feasibility of detecting individual tumor-infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high-resolution surface coil. Using a recently developed high-temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 microm)(3) at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin-expressing tumors by transferred OVA antigen-specific cytotoxic lymphocytes with low iron load.
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS, Université Paris 7-Denis Diderot, Paris, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Smirnov
authors.forename: Marie
authors.initials: M
authors.lastname: Poirier-Quinot
authors.forename: Claire
authors.initials: C
authors.lastname: Wilhelm
authors.forename: Elise
authors.initials: E
authors.lastname: Lavergne
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Ginefri
authors.forename: Béhazine
authors.initials: B
authors.lastname: Combadière
authors.forename: Olivier
authors.initials: O
authors.lastname: Clément
authors.forename: Luc
authors.initials: L
authors.lastname: Darrasse
authors.forename: Florence
authors.initials: F
authors.lastname: Gazeau
mesh_terms: D000818:Animals; D002460:Cell Line; D003287:Contrast Media; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005290:Ferric Compounds; D007089:Image Enhancement / Q000295:instrumentation*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods*; D008280:Magnetics / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000473:pathology*; D020556:Neutrophil Infiltration; D013601:T-Lymphocytes / Q000473:pathology*
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide