Pseudomonas aeruginosa: mannose sensitive hemagglutinin inhibits the growth of human hepatocarcinoma cells via mannose-mediated apoptosis.
A vaccine derived from the outer membrane proteins of the Gram-negative bacterium Pseudomonas aeruginosa has been shown to have immune modulatory properties. An inactivated mutant strain of P. aeruginosa with mannose sensitive hemagglutinin fimbria (PA-MSHA) has been used for adjuvant therapy for malignant cancer. In this study, the growth of human hepatocellular carcinoma Hep G2 and BEL-7402 cells is inhibited by PA-MSHA, but not by mannose-cleaved PA-MSHA. PA-MSHA-treated cells arrested in the S phase of the cell cycle and underwent apoptosis. We hypothesize that apoptosis induced by treatment of Hep G2 and BEL-7402 cells with PA-MSHA is mediated by the mannose residues of PA-MSHA and is propagated through the extrinsic apoptosis pathway directly through caspase-8. These data provide mechanistic details for the potential application of PA-MSHA-based treatment of hepatocellular carcinoma.
Cao
Zhenyuan
Z
Department of Intervention, The First Affiliated Clinic College of Harbin Medical University, Harbin, Heilongjiang, China.
Shi
Lijun
L
Li
Ying
Y
Wang
Jinghua
J
Wang
Dandan
D
Wang
Guangyou
G
Sun
Bo
B
Mu
Lili
L
Yang
Mingfei
M
Li
Hulun
H
D017209:Apoptosis / Q000187:drug effects*; D001428:Bacterial Vaccines / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology; D002453:Cell Cycle; D036781:Fimbriae Proteins / Q000276:immunology*; D005434:Flow Cytometry; D006388:Hemagglutinins / Q000276:immunology*; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology; D008358:Mannose / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D001428:Bacterial Vaccines; D006388:Hemagglutinins; D007155:Immunologic Factors; C000603452:mannosesensitive haemagglutinin, Pseudomonas aeruginosa; D036781:Fimbriae Proteins; D008358:Mannose