title: Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein.
abstract: We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in <or=24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and cheap, allowing for their routine use in clinical laboratories.
authors.affiliation: Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Orenburg 460056, Russia. mil-for@rambler.ru
authors.forename: Iuri B
authors.initials: IB
authors.lastname: Ivanov
authors.forename: Viktor A
authors.initials: VA
authors.lastname: Gritsenko
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D001792:Blood Platelets / Q000378:metabolism; D002908:Chronic Disease; D006801:Humans; D008297:Male; D011467:Prostate / Q000382:microbiology; D011472:Prostatitis / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000378:metabolism*; D001620:beta-Thromboglobulin / Q000037:antagonists & inhibitors*
chemical_list: D001426:Bacterial Proteins; C061615:PPBP protein, human; D001620:beta-Thromboglobulin