title: Mouse bone marrow mesenchymal stem cells acquire CD45-CD106+ immunophenotype only at later passages.
abstract: Classically, MSC are identified by a CD45-CD106+ phenotype. In this study, we found that mouse MSC achieve this characteristic phenotype only at later passages. With increasing passages, CD45 (hematopoietic marker) expression shifts to negativity, whereas CD106 (vascular cell adhesion molecule-1) expression becomes increasingly positive. These results demonstrate that MSC cells cultured from mouse bone marrow acquire a classical MSC immunophenotype (CD45-CD106+) in later passages.
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra, Malaysia.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ooi
authors.forename: R
authors.initials: R
authors.lastname: Ramasamy
authors.forename: S
authors.initials: S
authors.lastname: Vidyadaran
mesh_terms: D000818:Animals; D015415:Biomarkers; D001853:Bone Marrow; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000235:genetics; D016130:Immunophenotyping; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens / Q000235:genetics*; D059630:Mesenchymal Stem Cells; D051379:Mice; D023421:Models, Animal; D018215:Osteoblastoma; D010006:Osteoblasts; D010012:Osteogenesis; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics*
chemical_list: D015415:Biomarkers; D019010:Vascular Cell Adhesion Molecule-1; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human