Pulse electromagnetic fields effects on serum E2 levels, chondrocyte apoptosis, and matrix metalloproteinase-13 expression in ovariectomized rats.
Observe pulse electromagnetic fields (PEMFs) effects on ovariectomized (OVX) rats, to study the mechanisms of PEMFs therapy for postmenopausal osteoarthritis. Forty-eight female rats were exposed to PEMFs (PEMFs group), administrated E2 and placebo PEMFs (E group), or were treated with placebo PEMFs (OVX and Sham groups). The treatment duration was 30 days after which serum E2 levels, chondrocyte morphology, chondrocyte apoptosis and matrix metalloproteinases-13 expression in knee joint was analyzed. We observed differential chondrocyte formation in each group, and serum E2 content in the PEMFs and E group were significantly higher than the OVX group. The apoptosis index of chondrocytes and the positive index of MMP13 expression in the PEMFs group and E group were significantly lower than the OVX group. PEMFs has a systemic effect on estrogen metabolism in ovariectomized rats, then inhibit chondrocyte apoptosis and downregulate MMP13 expression of knee joint cartilage. It may be the mechanisms by which PEMFs therapy works for on postmenopausal osteoarthritis.
Luo
QingLu
Q
Department of Rehabilitation, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, People's Republic of China.
Li
Sha-Sha
SS
He
ChengQi
C
He
HongChen
H
Yang
Lin
L
Deng
Li
L
D000818:Animals; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects*; D002358:Cartilage, Articular / Q000378:metabolism / Q000528:radiation effects; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D015536:Down-Regulation / Q000528:radiation effects; D004574:Electromagnetic Fields; D004958:Estradiol / Q000097:blood*; D005260:Female; D007719:Knee Joint / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D010052:Ovariectomy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D004958:Estradiol; D053509:Matrix Metalloproteinase 13