title: Difference between the abilities of human Fcgamma receptor-expressing CV-1 cells to neutralize American and Asian genotypes of dengue virus 2.
abstract: Sera from patients involved in a Peruvian outbreak of dengue virus serotype 1 infection cross-neutralized the American genotype of dengue virus serotype 2 up to 100-fold more efficiently than they did the virulent Asian genotype of dengue virus serotype 2, as determined by a plaque reduction neutralization test (PRNT) with CV-1 fibroblasts modified to express human Fcgamma receptor CD32. The concordant preferential immune enhancement of the Asian genotype of dengue virus serotype 2 in human monocytes suggests that such a modification might strengthen the correlation between the PRNT titer and protection.
authors.affiliation: Department of Pathology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
authors.forename: W W Shanaka I
authors.initials: WW
authors.lastname: Rodrigo
authors.forename: W W I S
authors.initials: WW
authors.lastname: Rodrigo
authors.forename: D C
authors.initials: DC
authors.lastname: Alcena
authors.forename: Z
authors.initials: Z
authors.lastname: Kou
authors.forename: T J
authors.initials: TJ
authors.lastname: Kochel
authors.forename: K R
authors.initials: KR
authors.lastname: Porter
authors.forename: G
authors.initials: G
authors.lastname: Comach
authors.forename: R C
authors.initials: RC
authors.lastname: Rose
authors.forename: X
authors.initials: X
authors.lastname: Jin
authors.forename: J J
authors.initials: JJ
authors.lastname: Schlesinger
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D005838:Genotype; D006801:Humans; D009500:Neutralization Tests; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology*; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral; D017452:Receptors, IgG