High-throughput analysis of DNA fragments using a miniaturized CE system combined with a slotted-vial array sample introduction system.
An automated nanoliter sample introduction system was combined to a liquid-core waveguide (LCW)-based microfluidic CE system for high-throughput analysis of DNA fragments. The main component of the sample introduction system was a motor-driven plate, on which a circular array of bottom-slotted vials containing sample/buffer solutions was placed. A 7 cm-long LCW capillary served as both the sample probe and separation channel. The inlet terminal of the capillary could pass through the slots of the vials for electrokinetic sample introduction, and the capillary outlet was immersed in the solution of a reservoir, behind which a PMT facing directly to the outlet was positioned. A diode laser was used as excitation source for LCW LIF detection. Performance of the system was demonstrated through the separation of DNA fragments. Baseline separation was achieved for all 11 fragments of PhiX174-HaeIII digest DNA with a throughput of 33/h. Theoretical plate number for 603 bp fragment was 7.3x10(6)/m, corresponding to a plate height 0.14 microm. The detection limitation for 603 bp fragment was 0.4 ng/microL with a precision of 2.2% RSD for the peak height. Automated sample changing and introduction were achieved with only 0.3 nL gross sample consumption for each cycle.
Fan
Xiao-Feng
XF
Research Center for Analytical Sciences, Northeastern University, Shenyang, PR China.
Li
Qi
Q
Wang
Shi-Li
SL
Xu
Zhang-Run
ZR
Du
Wen-Bin
WB
Fang
Qun
Q
Fang
Zhao-Lun
ZL
D010584:Bacteriophage phi X 174 / Q000737:chemistry; D004279:DNA, Viral / Q000302:isolation & purification*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D008970:Molecular Weight; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry
D004279:DNA, Viral; D011108:Polymers