Infusion of cytomegalovirus specific cytotoxic T lymphocytes from a sero-negative donor can facilitate resolution of infection and immune reconstitution.
We report a stem cell transplant patient with a therapy-refractory cytomegalovirus (CMV) infection who received CMV-specific T cells from his sero-negative stem cell donor. This donor received the Towne strain CMV vaccine, and T cells were expanded using monocytes pulsed with pp65 overlapping peptides. CMV DNA decreased after the CTL infusion, and CMV-specific cytotoxicity increased. This strategy could be implemented in similar situations or with persistent viremia post-transplant.
Horn
Biljana
B
Department of Pediatrics, Blood and Marrow Transplant Program, University of California, San Francisco, CA.
Bao
Lei
L
Dunham
Kimberly
K
Stamer
Mindy
M
Adler
Stuart
S
Cowan
Morton
M
Lucas
Kenneth
K
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000628:therapy*; D022482:Cytomegalovirus Vaccines; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008297:Male; D010750:Phosphoproteins / Q000276:immunology*; D033581:Stem Cell Transplantation / Q000009:adverse effects; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology*; D055815:Young Adult
D022482:Cytomegalovirus Vaccines; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa