[Study on the association of cyclooxygenase-2 -765g>C and prostacyclin synthase C1117A polymorphisms and the risk of myocardial infarction in Uigur population of Xinjiang, China].
OBJECTIVE
The purpose of this study was to investigate the association of genetic polymorphism of cyclooxygenase-2 and prostacyclin synthase with myocardial infarction (MI) in Uigur population in Xinjiang.


METHODS
178 patients with MI and 175 healthy control subjects were detected on the genetic polymorphism of cyclooxygenase-2 and prostacyclin synthase by polymerase chain reaction-based restriction fragment length polymorphism. Other serum 6-keto-PGF1alpha concentration and biochemical indicators were detected in all the subjects.


RESULTS
(1) The genotype distributions of the control group and MI group were in the Hardy-Weinberg equilibrium. The frequencies of CC, CA and AA genotype of prostacyclin synthase were 75.84%, 17.42% and 6.74% in MI group while they were 64.57%, 28.29% and 9.14% in controls respectively. There was significant difference in frequencies of CC genotype and C allele as well as CA and AA genotypes between controls and MI cases. (2) The frequencies of -765GG, -765GC and -765CC genotype of cyclooxygenase-2 were 78.65%, 19.66% and 1.69% in MI group while they were 55.43%, 34.86% and 9.71% in controls respectively. There was significant difference in frequencies of three genotypes and alleles between the two groups (P < 0.05 or P < 0.01). (3) In combined genotype analysis, the genotype of PGIS CC + COX-2 -765GG was significantly higher in patients with MI than in control subjects (P < 0.05). The odds ratio estimated through combined analysis of the PGIS CC and COX-2 -765GG genotypes (OR = 3.87) markedly increased when compared with that estimated separately from the PGIS CC (OR = 1.72) or COX-2 -765GG (OR = 2.94) genotype. (4) There was a significant difference in serum 6-keto-PGF1alpha level between MI group and control group (P < 0.05), but there were no differences found in every genotype of PGIS and COX-2 gene (P > 0.05). In the cases with both COX-2 -765GG and PGIS CC genotypes, the serum 6-keto-PGF1alpha levels was lower than that of others (P < 0.05).


CONCLUSIONS
The CC genotype and C allele of prostacyclin synthase, -765GG genotype and G allele of COX-2 might serve as risk factors of MI of Uigur population in Xinjiang. Populations with both COX-2 -765GG and PGIS CC genotypes were more at risk with MI than others which might be resulted from the decreased serum 6-keto-PGF1alpha concentration. The -765CC genotype and C allele of COX-2 gene might have protective functions on MI among Uigur population in Xinjiang.
Xie
Xiang
X
Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
Ma
Yi-Tong
YT
Fu
Zhen-Yan
ZY
Yang
Yi-Ning
YN
Wang
Ying-Hong
YH
Chen
Bang-Dang
BD
Liu
Fen
F
D000368:Aged; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D051546:Cyclooxygenase 2 / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic
D003577:Cytochrome P-450 Enzyme System; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C472456:PTGIS protein, human