title: Cysteine-rich venom proteins from the snakes of Viperinae subfamily - molecular cloning and phylogenetic relationship.
abstract: Cysteine-rich proteins found in animal venoms (CRISP-Vs) are members of a large family of cysteine-rich secretory proteins (CRISPs). CRISP-Vs acting on different ion channels were found in venoms or mRNA (cDNA) encoding CRISP-Vs were cloned from snakes of three main families (Elapidae, Colubridae and Viperidae). About thirty snake CRISP-Vs were sequenced so far, however no complete sequence for CRISP-V from Viperinae subfamily was reported. We have cloned and sequenced for the first time cDNAs encoding CRISP-Vs from Vipera nikolskii and Vipera berus vipers (Viperinae). The deduced mature CRISP-V amino acid sequences consist of 220 amino acid residues. Phylogenetic analysis showed that viper proteins are closely related to those of Crotalinae snakes. The presence of CRISP-V in the V. berus venom was revealed using a combination of gel-filtration chromatography, electrophoresis and MALDI mass spectrometry. The finding of the putative channel blocker in viper venom may indicate its action on prey nervous system.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Laboratory of Neuropeptide Receptors, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Ramazanova
authors.forename: Vladislav G
authors.initials: VG
authors.lastname: Starkov
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Osipov
authors.forename: Rustam H
authors.initials: RH
authors.lastname: Ziganshin
authors.forename: Sergey Yu
authors.initials: SY
authors.lastname: Filkin
authors.forename: Victor I
authors.initials: VI
authors.lastname: Tsetlin
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Utkin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D014757:Viper Venoms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017819:Viperidae / Q000235:genetics / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D014757:Viper Venoms; D012313:RNA; D003545:Cysteine