title: [Measurement of airborne cladosporium spp antigens by aeroallergen immnoblotting and the seasonal fluctuation].
abstract: BACKGROUND
  Airborne Cladosporium spp (Clad) antigens were measured by aeroallergen immnoblotting, and analyzed the seasonal fluctuation of the antigens.
  
  
  METHODS
  Airborne Clad antigens (Clad) were transferred onto nitrocellulose membrane and treated with anti-Clad rabbit antiserum, then alkaline phosphatase-conjugated rabbit IgG. Finally, the spots from Clad antigens were visualized with BCIP/NBT substrate.
  
  
  RESULTS
  Clear purple spots from Clad antigens were seen on the airborne samples. Two peaks of spring and autumn were observed on the study of daily examination of the spots. Many spots from Clad antigens were mainly observed from afternoon to midnight at daily basis. No correlation between the number of the spots and the four weather elements was seen.
  
  
  CONCLUSIONS
  Spots from airborne Clad antigens can be obtained easily by use of an immunochemical technique. The great numbers of spores were seen in spring and autumn out of one year and from afternoon to midnight out of one day.
authors.affiliation: Yamagata Prefectural Institute of Public Health.
authors.forename: Michiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasueda
authors.forename: Akemi
authors.initials: A
authors.lastname: Saito
mesh_terms: D000388:Air / Q000032:analysis*; D000391:Air Microbiology; D000485:Allergens / Q000032:analysis*; D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis*; D002963:Cladosporium / Q000276:immunology*; D003101:Collodion; D015151:Immunoblotting / Q000379:methods*; D007120:Immunochemistry / Q000379:methods; D009172:Mycology / Q000379:methods*; D052638:Particulate Matter / Q000032:analysis*; D011817:Rabbits; D012621:Seasons; D013995:Time
chemical_list: D000485:Allergens; D000946:Antigens, Fungal; D052638:Particulate Matter; D003101:Collodion