title: Mapping of the spontaneous deletion in the Ap3d1 gene of mocha mice: fast and reliable genotyping.
abstract: BACKGROUND
  The mocha mouse carries a spontaneous deletion in the Ap3d1 gene, encoding the delta 1 subunit of the adaptor related protein complex 3, (Ap3d1), and subsequently lack the expression of functional AP-3. This leads to a deficiency in vesicle transport and storage, which affects neurotransmitter vesicle turnover and release in the central nervous system. Since the genomic sequence of the Ap3d1 gene of mocha mouse is not known, precise mapping of the deletion as well as reliable genotyping protocols are lacking.
  
  
  RESULTS
  We sequenced the Ap3d1 gene (HGNC GeneID: 8943) around the deletion site in the mocha mouse and revealed a 10639 bp deletion covering exon 2 to 6. Subsequently, new PCR primers were designed yielding a reliable genotyping protocol of both newborn and adult tissue. To examine the genotypes further, hippocampal neurons were cultured from mocha and control mice. Patch-clamp recordings showed that mocha neurons had a higher input resistance, and that autaptic EPSC in mocha cultures depressed faster and stronger as compared with control cultures.
  
  
  CONCLUSIONS
  Our study reports the sequence of the deleted part of the Ap3d1 gene in mocha mice, as well as a reliable PCR-based genotyping protocol. We cultured hippocampal neurons from control and mocha mice, and found a difference in input resistance of the neurons, and in the synaptic short-term plasticity of glutamatergic autapses showing a larger synaptic depression than controls. The described procedures may be useful for the future utilization of the mocha mouse as a model of defective vesicle biogenesis. Importantly, as genotyping by eye color is complicated in newborn mice, the designed protocol is so fast and reliable that newborn mice could rapidly be genotyped and hippocampal neurons dissociated and cultured, which is normally best done at P0-P2.
authors.affiliation: Synaptic Physiology Laboratory, Department of Physiology and Biophysics, Aarhus University, Ole Worms Allé Bldg 1185, DK-8000 Aarhus C, Denmark. mmh@fi.au.dk.
authors.forename: Kim Ryun
authors.initials: KR
authors.lastname: Drasbek
authors.forename: Mai Marie
authors.initials: MM
authors.lastname: Holm
authors.forename: Marion
authors.initials: M
authors.lastname: Delenclos
authors.forename: Kimmo
authors.initials: K
authors.lastname: Jensen