title: Efficacy and safety of five injectable anesthetic regimens for chronic blood collection from the anterior vena cava of Guinea pigs.
abstract: Despite several published methods of inducing surgical anesthesia in guinea pigs, viable methods of anesthesia for blood collection from the vena cava are inadequate. We compared 5 anesthesia regimens and their efficacy in inducing anesthesia for blood sampling in guinea pigs: ketamine-xylazine (30 and 2.5 mg/kg) administered subcutaneously, intramuscularly, or intraperitoneally; pentobarbital (37 mg/kg) administered intraperitoneally; and medetomidine (0.5 mg/kg) administered intramuscularly. Parameters measured included time to onset of anesthesia, time to recovery from anesthesia, and complete blood count (CBC) and serum chemistry values. CBC values did not differ among the 5 regimens, but serum glucose, BUN, phosphorous, and creatine phosphokinase levels varied among groups. Based on our data, intraperitoneal ketamine-xylazine appears to emerge as a preferable injectable anesthetic regimen in guinea pigs for blood collection from the anterior vena cava.
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Vi
authors.initials: V
authors.lastname: Dang
authors.forename: Saran
authors.initials: S
authors.lastname: Bao
authors.forename: Alida
authors.initials: A
authors.lastname: Ault
authors.forename: Catherine
authors.initials: C
authors.lastname: Murray
authors.forename: Joy
authors.initials: J
authors.lastname: McFarlane-Mills
authors.forename: Carmelo
authors.initials: C
authors.lastname: Chiedi
authors.forename: Marlon
authors.initials: M
authors.lastname: Dillon
authors.forename: John-Paul
authors.initials: JP
authors.lastname: Todd
authors.forename: Louis
authors.initials: L
authors.lastname: Detolla
authors.forename: Srinivas
authors.initials: S
authors.lastname: Rao
mesh_terms: D000700:Analgesics / Q000008:administration & dosage; D000758:Anesthesia / Q000662:veterinary; D000777:Anesthetics / Q000008:administration & dosage*; D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary*; D005260:Female; D006168:Guinea Pigs; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007649:Ketamine / Q000008:administration & dosage; D020926:Medetomidine / Q000008:administration & dosage; D014684:Venae Cavae; D014991:Xylazine / Q000008:administration & dosage
chemical_list: D000700:Analgesics; D000777:Anesthetics; D014991:Xylazine; D007649:Ketamine; D020926:Medetomidine