title: Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene.
abstract: We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to approximately 1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.
authors.affiliation: Department of Pathology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Shelly R
authors.initials: SR
authors.lastname: Gunn
authors.forename: Aswani R
authors.initials: AR
authors.lastname: Bolla
authors.forename: Lynn L
authors.initials: LL
authors.lastname: Barron
authors.forename: Mercedes E
authors.initials: ME
authors.lastname: Gorre
authors.forename: Mansoor S
authors.initials: MS
authors.lastname: Mohammed
authors.forename: David W
authors.initials: DW
authors.lastname: Bahler
authors.forename: Clemens H M
authors.initials: CH
authors.lastname: Mellink
authors.forename: Marinus H J
authors.initials: MH
authors.lastname: van Oers
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keating
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ferrajoli
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Coombes
authors.forename: Lynne V
authors.initials: LV
authors.lastname: Abruzzo
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Robetorye
mesh_terms: D000483:Alleles; D000951:Antigens, Neoplasm / Q000235:genetics*; D002872:Chromosome Deletion; D002892:Chromosomes, Human, Pair 22; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; C404944:PRAME protein, human