title: Genetic polymorphisms in TLR4, CR1 and Duffy genes are not associated with malaria resistance in patients from Baixo Amazonas region, Brazil.
abstract: The main purpose of this research was to analyze the relation of the genetic polymorphisms frequently expressed by antigen-presenting cells, erythrocytes and malaria susceptibility/resistance with the human malaria infection cases. The sample used consisted of 23 Plasmodium vivax (Pv)- and P. falciparum (Pf)-infected patients, and 21 healthy individuals as a control group, from the Baixo Amazonas population in Pará, Brazil. The Asp299Gly polymorphisms in the Toll-like receptor 4 (TLR4), and Gly42Asp, Arg89Cys, Ala100Thr, and T-33C in the Duffy gene (FY) were analyzed by restriction fragment length polymorphism-polymerase chain reaction. The Lys1590Glu and Arg1601Gly polymorphisms in the complement receptor type 1 (CR1) were analyzed by DNA sequencing. According to the results obtained and statistical analysis considering a significance level or alpha = 0.01, we conclude that the low heterozygote frequency (2.27%) for the Asp299Gly mutation, detected in the TLR4 gene, is not related to the Pv and Pf infections in the patients analyzed. Also, the promoter region GATA-1 analysis of the FY gene in the Pv-infected patients showed that the heterozygote frequency for the T-33C mutation (11.36% of the infected patients and 20.45% of the control patients) is not related to infection resistance. Regarding the CR1 gene, the observed heterozygote frequency (9.09%) for the Arg1601Gly mutation in Pf-infected patients when compared to heterozygote frequency in the control group (18.18%) suggests that there is no correlation with infection resistance.
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, Ribeirão Preto, SP, Brasil. simone@uea.edu.br
authors.forename: S C
authors.initials: SC
authors.lastname: Soares
authors.forename: K
authors.initials: K
authors.lastname: Abé-Sandes
authors.forename: V B
authors.initials: VB
authors.lastname: Nascimento Filho
authors.forename: F M F
authors.initials: FM
authors.lastname: Nunes
authors.forename: W A
authors.initials: WA
authors.lastname: Silva
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004375:Duffy Blood-Group System / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008288:Malaria / Q000453:epidemiology / Q000235:genetics*; D009154:Mutation; D010963:Plasmodium falciparum / Q000472:pathogenicity; D010966:Plasmodium vivax / Q000472:pathogenicity; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011956:Receptors, Cell Surface / Q000235:genetics*; D017463:Receptors, Complement 3b / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C473745:ACKR1 protein, human; C509991:CR1 protein, human; D004375:Duffy Blood-Group System; D011956:Receptors, Cell Surface; D017463:Receptors, Complement 3b; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4