[The analysis of hepatitis B virus genetic characterization and ratio of mutation in low-age group of HuNan].
OBJECTIVE
In order to investigate the characterization of mutation and genotype distributing in the younger group which was under the universal vaccination. The sequence of HBV was analyzed to offer the information to control and prevention in the area.


METHODS
Young person's sera with positive HBsAg are collected, and the Large S sequence of HBV including preS and S gene are amplified and sequenced. The genotype and serotype were determined by clastwal with the standard genotype sequence. And one virus complete genome is amplified.


RESULTS
The virus gene are successful amplified from the 33 sera. The sequence result indicate the 30 of 33 (90.9%) HBV genotype is B and 3 of 33 (9.0%) is C. The HBV serotype including ayw (1), adr (3), adw (29), 5 of 33 mutated in the "a" dominant of HBV, and the percentage is 15.2% . The HBV full length gene of serum number of 5856 is amplified and sequenced. Its genotype is B, serotype is adw and length is 3215 base.


CONCLUSIONS
The dominant genotype of HuNan is B, and the dominant serotype is adw.
Bian
Tao
T
Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Zhang
Yong
Y
Cao
Yan-Qiang
YQ
Wang
Ji-Jie
JJ
Shen
Li-Ping
LP
Wang
Feng
F
Wang
Yue
Y
Bi
Sheng-Li
SL
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D009154:Mutation; D010802:Phylogeny
D006514:Hepatitis B Surface Antigens