Effect of heme oxygenase–endogenous carbon monoxide on mortality during septic shock in rats.
OBJECTIVE
To investigate the effect of HO–CO on mortality during septic shock in rats.


METHODS
Eighty rats were randomly divided into group-control, group ZnPP-IX, group SS, and group LZ.


RESULTS
Death was significantly lower in group SS than in group LZ (P < 0.05). The MAP was significantly higher in the group LZ than in the group SS (P < 0.05). ALT, AST, Cr and BUN, MDA, and the lung EB contents, were significantly lower in the group SS than that in the group LZ (all P < 0.05). And CO and SOD activities were significantly higher in group SS than that in the group LZ (all P < 0.05). HO-1 mRNA, and HO-1 protein were significantly lower in the group LZ than in the group SS (P < 0.05), whereas HO-2 mRNA, and HO-2 protein were not significantly different among four groups (all P > 0.05).


CONCLUSIONS
The increased oxidative stress and end-organ damage is related to mortality during septic shock; while the hypotension partly contributing to HO-1 protein and CO has no obvious relation with it.
Yu
J-b
JB
Department of Anesthesiology, Tianjin Nankai Hospital, China. jianboyu99@yahoo.com.cn
Yao
S-l
SL
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D002248:Carbon Monoxide / Q000097:blood / Q000502:physiology*; D003404:Creatinine / Q000097:blood; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism / Q000502:physiology*; D051547:Heme Oxygenase-1 / Q000378:metabolism / Q000502:physiology*; D007022:Hypotension / Q000503:physiopathology*; D008070:Lipopolysaccharides; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000139:chemically induced / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation
D008070:Lipopolysaccharides; D011524:Protoporphyrins; D012333:RNA, Messenger; C017803:zinc protoporphyrin; D008315:Malondialdehyde; D002248:Carbon Monoxide; D003404:Creatinine; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase