Immunohistochemical evaluation of the inflammatory response in periodontal disease.
In order to contribute to the knowledge of the pathogenesis of periodontal disease, an immunohistochemical analysis of the density of inflammatory mononucleated cells and the number of dendritic cells was performed using anti-CD4, anti-CD20, anti-CD25, anti-CD68 and anti-protein S-100 antibodies in 17 cases of chronic gingivitis (CG) and 25 of chronic periodontitis (CP). The CD4+ and CD68+ cells exhibited a diffuse distribution in the connective tissue. CD20+ cell distribution was predominantly in groups and the CD25+ cells exhibited a diffuse or focal distribution. The S-100+ cells were identified in the epithelium and the lamina propria, exhibiting distinct morphology and number. The statistical analysis showed no significant differences (p>0.05) between CG and CP regarding the density of the CD4+ and CD20+ cells and the number of S-100+ cells. However, significant differences (p<0.05) were found between the groups in the density of CD25+ and CD68+ cells . The density of macrophages was greater in CG and the level of cellular activation of the lymphocyte infiltrate was greater in CP. No differences were detected between the aforementioned conditions regarding the density of the T and B lymphocytes and to the number of the dendritic cells.
Lins
Ruthinéia Diógenes Alves Uchôa
RD
Department of Periodontics, University of the State of Paraíba, Campina Grande, PB, Brazil.
Figueiredo
Cláudia Roberta Leite Vieira
CR
Queiroz
Lélia Maria Guedes
LM
da Silveira
Ericka Janine Dantas
EJ
Freitas
Roseana de Almeida
Rde A
D015703:Antigens, CD / Q000032:analysis; D018951:Antigens, CD20 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D002452:Cell Count; D048430:Cell Shape; D002908:Chronic Disease; D055113:Chronic Periodontitis / Q000276:immunology / Q000473:pathology*; D003238:Connective Tissue / Q000276:immunology / Q000473:pathology; D003713:Dendritic Cells / Q000473:pathology; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005891:Gingivitis / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000473:pathology; D009092:Mucous Membrane / Q000276:immunology / Q000473:pathology; D009418:S100 Proteins / Q000032:analysis; D000097382:CD68 Molecule
D015703:Antigens, CD; D018951:Antigens, CD20; D015214:Antigens, Differentiation, Myelomonocytic; D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit; D009418:S100 Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule