title: Expression of cardiac function genes in adult stem cells is increased by treatment with nitric oxide agents.
abstract: Mesenchymal stem cells (MSCs) have received special attention for cardiomyoplasty because several studies have shown that they differentiate into cardiomyocytes both in vitro and in vivo. Nitric oxide (NO) is a free radical signaling molecule that regulates several differentiation processes including cardiomyogenesis. Here, we report an investigation of the effects of two NO agents (SNAP and DEA/NO), able to activate both cGMP-dependent and -independent pathways, on the cardiomyogenic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived stem cells (ADSCs). The cells were isolated, cultured and treated with NO agents. Cardiac- and muscle-specific gene expression was analyzed by indirect immunofluorescence, flow cytometry, RT-PCR and real-time PCR. We found that untreated (control) ADSCs and BM-MSCs expressed some muscle markers and NO-derived intermediates induce an increased expression of some cardiac function genes in BM-MSCs and ADSCs. Moreover, NO agents considerably increased the pro-angiogenic potential mostly of BM-MSCs as determined by VEGF mRNA levels.
authors.affiliation: Núcleo de Cardiomioplastia Celular, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição 1155, Curitiba 80215-901, Brazil.
authors.forename: Carmen K
authors.initials: CK
authors.lastname: Rebelatto
authors.forename: Alessandra M
authors.initials: AM
authors.lastname: Aguiar
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Senegaglia
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Aita
authors.forename: Paula
authors.initials: P
authors.lastname: Hansen
authors.forename: Fabiane
authors.initials: F
authors.lastname: Barchiki
authors.forename: Crisciele
authors.initials: C
authors.lastname: Kuligovski
authors.forename: Márcia
authors.initials: M
authors.lastname: Olandoski
authors.forename: José A
authors.initials: JA
authors.lastname: Moutinho
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Brofman
authors.forename: Lia S
authors.initials: LS
authors.lastname: Nakao
authors.forename: Alejandro
authors.initials: A
authors.lastname: Correa
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000368:Aged; D015703:Antigens, CD / Q000235:genetics; D018421:Cardiomyoplasty; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D018031:Connexin 43 / Q000235:genetics; D015870:Gene Expression; D005819:Genetic Markers; D006321:Heart / Q000502:physiology; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics; D032383:Myocytes, Cardiac / Q000166:cytology*; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D015703:Antigens, CD; D018031:Connexin 43; D005819:Genetic Markers; D006834:Hydrazines; D009124:Muscle Proteins; D020030:Nitric Oxide Donors; C110881:S-nitro-N-acetylpenicillamine; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D010396:Penicillamine