title: In vitro behavior of neural stem cells in response to different chemical functional groups.
abstract: Neural stem cells (NSCs) cultured on glass surfaces modified by different chemical groups, including hydroxyl (-OH), sulfonic (-SO3H), amino (-NH2), carboxyl (-COOH), mercapto (-SH) and methyl (-CH3) groups, are shown here to commit to phonotypes with extreme sensitivity to surface chemical groups. The adhering NSCs at the level of single cells exhibited morphological changes in response to different chemical groups. NSCs on -SO(3)H surfaces had the largest contact area and the most flattened morphology, while those on -CH(3) surfaces exhibited the smallest contact area and the most rounded morphology. After 5 days of culture, the migration of NSCs from their original aggregates onto these test surfaces followed the trend: -NH2>-COOH=-SH>>-SO3H>-CH3>-OH. The expression of specific markers, including nestin, beta-Tubulin-III, glial fibrillary acidic protein and O4, were used to examine NSCs lineage specification. The -SO3H surfaces favored NSCs differentiation into oligodendrocytes, while NSCs in contact with -COOH, -NH2, -SH and -CH3 had the ability to differentiate into neurons, astrocytes and oligodendrocytes. Compared to -COOH surfaces, -NH2 seemed to promote neuronal differentiation. These chemically modified surfaces exhibited regulation of NSCs on adhesion, migration and differentiation potential, providing chemical means for the design of biomaterials to direct NSCs lineage specification for neural tissue engineering.
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, Department of Material Science and Engineering, Tsinghua University, Beijing 100084, China. renyj04@mails.tsinghua.edu.cn
authors.forename: Yong-Juan
authors.initials: YJ
authors.lastname: Ren
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Zi-You
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Fu-Zhai
authors.initials: FZ
authors.lastname: Cui
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: An
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007287:Inorganic Chemicals / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D010641:Phenotype; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D014867:Water
chemical_list: D007287:Inorganic Chemicals; D012997:Solvents; D014867:Water