title: Influence of polymer structure and biodegradation on DNA release from silk-elastinlike protein polymer hydrogels.
abstract: Silk-elastinlike protein polymers (SELPs) of varying ratios and lengths of silk and elastin blocks capable of hydrogel formation were evaluated as matrices for controlled delivery of plasmid DNA. Influence of polymer structure, ionic strength of the media and gelation time on DNA release from two structurally related hydrogels, SELP-47K and SELP-415K, was evaluated. The influence of elastase-induced degradation on the swelling behavior and DNA release from these hydrogels was investigated. Results indicate that release is a function of polymer structure, concentration and cure time. SELP-415K which has twice the number of elastin units as that of SELP-47K demonstrated higher release than that of SELP-47K. DNA release from these hydrogels is an inverse function of polymer concentration and cure time, with higher release observed at lower polymer concentration and shorter cure time. Results indicate that ionic strength of the media governs the rate of release. An increase in swelling ratio was observed in the presence of elastase at 12 wt.% composition for both SELP analogs. Release in the presence of elastase was enhanced due to increased swelling ratio and loss of hydrogel integrity. These studies allude to the utility of recombinant techniques to control plasmid DNA release and biodegradation in SELP hydrogels.
authors.affiliation: Department of Bioengineering, University of Maryland, College Park, MD, USA.
authors.forename: David
authors.initials: D
authors.lastname: Hwang
authors.forename: Vikas
authors.initials: V
authors.lastname: Moolchandani
authors.forename: Ramesh
authors.initials: R
authors.lastname: Dandu
authors.forename: Mohamed
authors.initials: M
authors.lastname: Haider
authors.forename: Joseph
authors.initials: J
authors.lastname: Cappello
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ghandehari
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D004549:Elastin / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D009994:Osmolar Concentration; D010196:Pancreatic Elastase / Q000737:chemistry; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D005353:Fibronectins; D020100:Hydrogels; D011993:Recombinant Fusion Proteins; C089172:silk-like protein polymer; D004247:DNA; D004549:Elastin; D005349:Fibroins; D010196:Pancreatic Elastase