title: The investigation on the value of repeat and combination test of ACA and anti-beta2-GPI antibody in women with recurrent spontaneous abortion.
abstract: OBJECTIVE
  In order to investigate the value of anticardiolipin antibodies (ACA) and anti-beta2-GPI antibodies detection in screening autoimmune type recurrent spontaneous abortion and its clinic application in antiphospholipid syndrome diagnosis, we adopt repeat combined ACA and anti-beta2-GPI antibodies detection in this study.
  
  
  METHODS
  Sera were collected from patients and work-up was done for detection of ACA and anti-beta2-GPI antibodies by enzyme-linked immunosorbent assay (ELISA). The work-up was done for detection of antibodies once in every 6 weeks for 14 times consecutively.
  
  
  RESULTS
  The repeated and combined detection of ACA and anti-beta2-GPI antibodies detection could raise the positivity rate up to 21.8% (P < 0.05) in comparison with positive for ACA alone (14.1%), positive for anti-beta2-GPI alone (3.1%), and concurrently positive for both ACA and anti-beta2-GPI antibodies (4.6%). In 91 confirmed positive antiphospholipid antibodies (APA) patients, with more frequent screening for ACA and anti-beta2-GPI antibodies, more patients with APA were found. The positive rate of five and more screenings was over 81.32%, which was statistically significant (P < 0.05), in comparison with that of four or less screenings (68.13%).
  
  
  CONCLUSIONS
  Our data implied that it would be appropriate to take over five or more screenings of combined ACA and anti-beta2-GPI antibodies detection in suspect patients to facilitate the positive diagnostic rate for autoimmune type RSA.
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
authors.forename: Shi Hua
authors.initials: SH
authors.lastname: Bao
authors.forename: Xi Peng
authors.initials: XP
authors.lastname: Wang
authors.forename: Qi De
authors.initials: QD
authors.lastname: Lin
authors.forename: Wen
authors.initials: W
authors.lastname: Di
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Chuan Wei
authors.initials: CW
authors.lastname: Ding
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000022:Abortion, Spontaneous / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D011247:Pregnancy; D053482:beta 2-Glycoprotein I / Q000097:blood*
chemical_list: D017153:Antibodies, Anticardiolipin; D053482:beta 2-Glycoprotein I