Interference in clinical chemistry assays by the hemoglobin-based oxygen carrier, Hemospan.
OBJECTIVE
To evaluate interference by the hemoglobin-based oxygen carrier Hemospan on clinical laboratory assays.


METHODS
Interfering Hemospan concentrations were determined for general chemistry and cardiac marker analytes in pooled serum and the corresponding hemolysis index was calculated.


RESULTS
Hemospan did not interfere with 20 of 35 analytes. Hemospan produced a negative interference in serum creatinine, amylase, alkaline phosphatase, uric acid, and GGT assays and a positive interference in serum phosphate, LDH, iron, triglycerides, total protein, AST, cholesterol, magnesium, and albumin assays, and appeared to positively bias the serum cardiac troponin I (cTnI) assay only when cTnI is present in the sample.


CONCLUSIONS
We present a report of assays affected by Hemospan and the threshold concentrations for interference. This study highlights the importance of interference studies in understanding the effects of hemoglobin-based oxygen carriers on results reported by the clinical laboratory.
Cameron
Scott J
SJ
Department of Pathology, Clinical Chemistry Division, Johns Hopkins Medical Institutions, Meyer B-125 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Gerhardt
Gregory
G
Engelstad
Michael
M
Young
Mark A
MA
Norris
Edward J
EJ
Sokoll
Lori J
LJ
D001774:Blood Chemical Analysis / Q000592:standards*; D001802:Blood Substitutes; D002624:Chemistry, Clinical / Q000379:methods / Q000592:standards*; D003951:Diagnostic Errors; D006454:Hemoglobins; D006801:Humans; D008301:Maleimides; D011092:Polyethylene Glycols; D015203:Reproducibility of Results
D001802:Blood Substitutes; D006454:Hemoglobins; D008301:Maleimides; C493836:maleimide-polyethylene glycol-modified hemoglobin, MP4; D011092:Polyethylene Glycols