title: Real-time PCR for quantification of eleven individual Fusarium species in cereals.
abstract: Contamination of cereals with Fusarium species is one of the major sources of mycotoxins in food and feed. Quantification of biomass of Fusarium species is essential to understand the interactions of individual species in disease development. In this study quantitative real-time PCR assays based on the elongation factor 1 alpha (EF1alpha) gene for the 11 Fusarium species F. graminearum, F. culmorum, F. poae, F. langsethiae, F. sporotrichioides, F. equiseti, F. tricinctum, F. avenaceum, F. verticillioides, F. subglutinans and F. proliferatum were developed and tested on 24 wheat and 24 maize field samples. The assays were found to be specific and sensitive. Generally, the results from the quantitative real-time PCR assays corresponded well with mycotoxin data of the field samples.
authors.affiliation: Aarhus University, Faculty of Agricultural Sciences, Department of Integrated Pest Management, Denmark. mogens.nicolaisen@agrsci.dk <mogens.nicolaisen@agrsci.dk>
authors.forename: Mogens
authors.initials: M
authors.lastname: Nicolaisen
authors.forename: Skaidre
authors.initials: S
authors.lastname: Suproniene
authors.forename: Linda Kaergaard
authors.initials: LK
authors.lastname: Nielsen
authors.forename: Irene
authors.initials: I
authors.lastname: Lazzaro
authors.forename: Niels Henrik
authors.initials: NH
authors.lastname: Spliid
authors.forename: Annemarie Fejer
authors.initials: AF
authors.lastname: Justesen
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D002523:Edible Grain / Q000382:microbiology*; D005516:Food Microbiology; D005670:Fusarium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009183:Mycotoxins / Q000032:analysis; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D009183:Mycotoxins; D020648:Peptide Elongation Factor 1