Non-invasive imaging of stem cells by scanning ion conductance microscopy: future perspective.
The most valuable property of stem cells (SCs) is their potential to differentiate into many or all cell types of the body. So far, monitoring SC differentiation has only been possible after cells were fixed or destroyed during sample preparation. It is, however, important to develop nondestructive methods of monitoring SCs. Scanning ion conductance microscopy (SICM) is a unique imaging technique that uses similar principles to the atomic force microscope, but with a pipette for the probe. This allows scanning of the surface of living cells noninvasively and enables measurement of cellular activities under more physiological conditions than is possible with other high-resolution microscopy techniques. We report here the novel use of the SICM for studying SCs to assess and monitor the status of SCs and various cell types differentiated from SCs.
Gorelik
Julia
J
National Heart and Lung Institute, Imperial College London, London, United Kingdom. j.gorelik@imperial.ac.uk
Ali
Nadire N
NN
Sheikh Abdul Kadir
Siti H
SH
Lab
Max
M
Stojkovic
Petra
P
Armstrong
Lyle
L
Sviderskaya
Elena V
EV
Negulyaev
Yuri A
YA
Klenerman
David
D
Bennett
Dorothy C
DC
Lako
Majlinda
M
Harding
Sian E
SE
Stojkovic
Miodrag
M
Korchev
Yuri E
YE
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004867:Equipment Design; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009432:Neural Crest / Q000473:pathology; D009474:Neurons / Q000166:cytology; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D013499:Surface Properties