title: Malathion-induced alteration of the antioxidant defence system in kidney, gill, and intestine of Carassius auratus gibelio.
abstract: Pesticides such as malathion, commonly used in agriculture and households, are toxic substances that lead to reactive oxygen species generation, which harms organisms. Ecotoxicological consequences of malathion, particularly its effects on antioxidants in fish, are not well understood. Thus, we investigated the effects of malathion (0.05 mg/L) on lipid peroxidation and antioxidant systems in Carassius auratus gibelio kidney, intestine, and gills following exposure times of 1, 2, 3, and 6 days. The lipid peroxidation and antioxidative defense mechanisms display different responses in investigated tissues. The lipid peroxidation was increased in all investigated tissues, especially after 1 day of malathion administration. Changes in reduced glutathione levels have been registered, mainly after 6 days of pesticide exposure. The modulation in the activities of antioxidant enzymes, catalase, gluthatione peroxidase, glutathione reductase, and glutathione-S-transferase was time and tissue specific. The investigated parameters can be used as biomarkers of fish exposure to malathion.
authors.affiliation: Molecular Biology Center, Department of Biochemistry, University of Bucharest, 91-95 Spl. Independentei, 050095 Bucharest, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Huculeci
authors.forename: Diana
authors.initials: D
authors.lastname: Dinu
authors.forename: Andreea Cristina
authors.initials: AC
authors.lastname: Staicu
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Munteanu
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005880:Gills / Q000187:drug effects / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008294:Malathion / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000975:Antioxidants; D007306:Insecticides; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D008294:Malathion