Solid substrate-room temperature phosphorimetry for the determination of trace lead using p-nitro-phenyl-fluorone-multi-wall carbon nanotubes-Tween-80 micellae compound and diagnosis about human diseases.
The structures of multi-wall carbon nanotubes (MWNTs) were modified by H(2)SO(4)-HNO(3) and H(2)SO(4)-H(2)O(2), respectively. The corresponding products were water-soluble MWNTs-A and MWNTs-B. According to the experiment, it was found that MWNTs-B could emit stable solid substrate-room temperature phosphorescence (RTP) on the surface of paper with Ag(+) as perturber. Under the conditions of 70 degrees C and 15 min, MWNTs-B can react with Tween-80 and p-nitro-phenyl-fluorone (R) to form R-MWNTs-B-Tween-80 micellae compound, which could emit RTP of R and MWNTs-B on the surface of paper, respectively. Pb(2+) could cause the RTP of R and MWNTs-B enhanced sharply, respectively. DeltaI(p) is directly proportional to the content of Pb(2+). A new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace Pb(2+) has been established based on R-MWNTs-B-Tween-80 micellae compound containing double luminescent molecule. The detection limit of this method were 0.035 ag Pb(2+) spot(-1) (8.8 x 10(-17) g Pb(2+) ml(-1), MWNTs-B) and 0.028 ag Pb(2+) spot(-1) (7.1 x 10(-17) g Pb(2+) ml(-1), R). This method is of high sensitivity, good selectivity, high precision and accuracy. It could be applied to determine trace Pb(2+) in serum samples at wavelength of 453.7/623.0 nm (R) or 475.9/645.0 nm (MWNTs-B) with satisfactory results, showing that SS-RTP has flexibility and utility value. Simultaneously, this method can be used to diagnose human diseases. The reaction mechanism for the determination of trace Pb(2+) by SS-RTP based on R-MWNTs-B-Tween-80 micellae compound containing double luminescent molecule was also discussed.
Yang
Tianlong
T
Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
Liu
Zhenbo
Z
Liu
Jiaming
J
Liu
Haizhu
H
Huang
Yahong
Y
Liu
Jianqin
J
Chen
Xuebing
X
Zhao
Yibing
Y
D000143:Acids; D004194:Disease; D005452:Fluoresceins / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D007477:Ions; D007854:Lead / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D008823:Micelles; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D000143:Acids; D005452:Fluoresceins; D007202:Indicators and Reagents; D007477:Ions; D008823:Micelles; D037742:Nanotubes, Carbon; D011136:Polysorbates; D007854:Lead