Characterization of the interferon regulatory factor 3-mediated antiviral response in a cell line deficient for IFN production.
The innate cellular response to virus particle entry in non-immune cells requires the transcriptional activity of interferon regulatory factor 3 (IRF-3), but not production of type I interferon (IFN). Here, we characterize the IFN-independent innate cellular response to virus-derived stimuli in Vero cells, a monkey kidney epithelial cell line deficient for IFN production. We provide evidence that Vero cells are deficient in their ability to mount an IRF-3-dependent, IFN-independent antiviral response against either incoming virus particles or polyinosinic:polycytidylic acid (pIC), a dsRNA mimetic. We further demonstrate that abundance of IRF-3 protein is a determinant in the pIC-mediated antiviral signalling pathway. These observations further characterize the permissive nature of Vero cells to viral infection, and highlight the crucial involvement of IRF-3 in the innate antiviral response.
Chew
Tracy
T
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
Noyce
Ryan
R
Collins
Susan E
SE
Hancock
Meaghan H
MH
Mossman
Karen L
KL
D000818:Animals; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000276:immunology* / Q000502:physiology / Q000528:radiation effects; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000276:immunology*; D007372:Interferons / Q000096:biosynthesis*; D011070:Poly I-C / Q000494:pharmacology; D029082:Sendai virus / Q000187:drug effects / Q000276:immunology* / Q000502:physiology / Q000528:radiation effects; D017422:Sequence Analysis, DNA; D014466:Ultraviolet Rays; D014709:Vero Cells; D038301:Virus Inactivation / Q000187:drug effects / Q000528:radiation effects; D014779:Virus Replication / Q000187:drug effects / Q000528:radiation effects
C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; D007372:Interferons; D011070:Poly I-C