title: Phage lysin LysK can be truncated to its CHAP domain and retain lytic activity against live antibiotic-resistant staphylococci.
abstract: A truncated derivative of the phage endolysin LysK containing only the CHAP (cysteine- and histidine-dependent amidohydrolase/peptidase) domain exhibited lytic activity against live clinical staphylococcal isolates, including methicillin-resistant Staphylococcus aureus. This is the first known report of a truncated phage lysin which retains high lytic activity against live staphylococcal cells.
authors.affiliation: Teagasc, Moorepark Food Research Centre, Fermoy, County Cork, Ireland.
authors.forename: Marianne
authors.initials: M
authors.lastname: Horgan
authors.forename: Gary
authors.initials: G
authors.lastname: O'Flynn
authors.forename: Jennifer
authors.initials: J
authors.lastname: Garry
authors.forename: Jakki
authors.initials: J
authors.lastname: Cooney
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Olivia
authors.initials: O
authors.lastname: McAuliffe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology*; D024881:Drug Resistance, Bacterial; D009088:Mucoproteins / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D009088:Mucoproteins; C010393:lysin, gastropoda