title: Cytotoxicity and apoptosis induction of weisiensin B isolated from Rabdosia weisiensis C.Y. Wu in human hepatoma cells.
abstract: Weisiensin B, a new ent-kaurane diterpenoid, was isolated from traditional Chinese herb Rabdosia weisiensis C.Y. Wu. In this study, cytotoxicity of weisiensin B was tested on four different tumor cell lines and the effect of growth inhibition and apoptosis in BEL-7402 cell line were investigated in vitro. The results indicated that weisiensin B had significant antiproliferation activity on the four cell lines. Further study on BEL-7402 cells involving Hoechst 33258 stain and DNA fragmentation assay revealed the characteristic apoptotic features of nuclear and DNA ladder formation. Flow cytometric (FCM) analysis with propidium iodide (PI) staining demonstrated that BEL-7402 cells treated with weisiensin B were arrested in G(2)/M phase. The results demonstrated that a significant fraction of weisiensin B-treated cells died by an apoptotic pathway in BEL-7402 cells.
authors.affiliation: College of Life Sciences, Northwest Normal University, Lanzhou, China. dinglan@nwnu.edu.cn
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.forename: Shi-Dong
authors.initials: SD
authors.lastname: Zhang
authors.forename: Dong-Juan
authors.initials: DJ
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Qi-Yin
authors.initials: QY
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C539120:weisiensin B