title: MLVA genotyping of human Brucella isolates from Peru.
abstract: Recent human Brucella melitensis isolates from Peru were genotyped by multiple locus variable number repeat analysis. All 24 isolates originated from hospitalized patients living in the central part of Peru and consisted of six genomic groups comprising two to four isolates and nine unique genotypes. The isolates were most closely related to the two previously genotyped isolates from Mexico, with a maximum distance of 2 to 4. The Peruvian strains were clearly distinct from the East and West Mediterranean groups of B. melitensis genotypes, suggesting that they may constitute a unique Latin American cluster.
authors.affiliation: KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. h.smits@kit.nl
authors.forename: Henk L
authors.initials: HL
authors.lastname: Smits
authors.forename: Benjamin
authors.initials: B
authors.lastname: Espinosa
authors.forename: Rosa
authors.initials: R
authors.lastname: Castillo
authors.forename: Eric
authors.initials: E
authors.lastname: Hall
authors.forename: Alfredo
authors.initials: A
authors.lastname: Guillen
authors.forename: Milagros
authors.initials: M
authors.lastname: Zevaleta
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
authors.forename: Paolo
authors.initials: P
authors.lastname: Melendez
authors.forename: Carlos
authors.initials: C
authors.lastname: Guerra
authors.forename: Angelika
authors.initials: A
authors.lastname: Draeger
authors.forename: Alessandro
authors.initials: A
authors.lastname: Broglia
authors.forename: Karsten
authors.initials: K
authors.lastname: Nöckler
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics*; D002006:Brucellosis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006041:Goats / Q000382:microbiology; D006801:Humans; D010568:Peru; D016133:Polymerase Chain Reaction; D012008:Recurrence; D012307:Risk Factors; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers