[Cloning and phylogenetic analysis of the entire gene of an H1N1 subtype swine influenza virus isolated from Guangdong Province].
To study the genetic variation and evolutionary characteristics of H1N1 swine influenza virus, all the eight genes of LM were amplified by RT- PCR, cloned into pMD18-T vector and sequenced respectively. The results showed that neither insertion nor deletion was observed in nucleotides of LM. The amino acids sequence of cleavage site of HA is IPSIQSR decrease G, suggesting that LM did not have the molecular characteristics of high pathogen. HA had highly conservative N-glycosylation site at position 11, 23, 87 and 276 sites of HA1, and two more at position 154 and 213 sites of HA2. NA had highly conservative N-glycosylation site at position 58, 63, 68, 88, 146, and two more at position 44 and 235 sites, which might be one molecular characteristics of H1N1 subtype of SIV. The results of Bast showed HA gene had high homology to the strain of 'human-like' SIV (99%), while others had high homology to the 'classical' SIV. So it is inferred that HA of LM might originate from human-like linage swine influenza virus, while others might originate from 'classical' swine influenza virus.
Liu
Da-Fei
DF
National Key Laboratory of Veterinary Biotechnology / Avian Influenza Lab of Ministry of Agriculture, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Liu
Ming
M
Liu
Chun-Guo
CG
Yang
Tao
T
Liu
Da-Cheng
DC
D000818:Animals; D003001:Cloning, Molecular; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000235:genetics*; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase