Rosmarinic acid and antioxidant enzyme activities in Lavandula vera MM cell suspension culture: a comparative study.
The growth and intracellular protein content of lavender (Lavandula vera MM) cell suspension culture was followed along with some antioxidant defense system members-non-enzymatic (rosmarinic acid) and enzymatic [superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6)]. It was found that the media content and the cultivation mode strongly influenced the production of plant defense compounds as well as the ratio between non-enzymatic and enzymatic ones. The bioreactor culture contains about two times more rosmarinic acid, superoxide dismutase, and catalase compared to the shake-flask cultivation. These findings are discussed with respect to the relative stress levels and plant antioxidant orchestra system. It was concluded that investigated defense system components (enzymatic and non-enzymatic) were closely associated in a complex balance. The three isoenzyme forms of SOD (Cu/ZnSOD, FeSOD, and MnSOD) in the cells of Lavandula vera were revealed by polyacrylamide gel electrophoresis analysis, and the FeSOD isoform exhibited highest activity.
Georgiev
Milen
M
Department of Microbial Biosynthesis and Biotechnologies-Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, 4002 Plovdiv, Bulgaria. milengeorgiev@gbg.bg
Abrashev
Radoslav
R
Krumova
Ekaterina
E
Demirevska
Klimentina
K
Ilieva
Mladenka
M
Angelova
Maria
M
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002934:Cinnamates / Q000378:metabolism*; D053630:Depsides / Q000378:metabolism*; D027523:Lavandula / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D000097123:Rosmarinic Acid
D000975:Antioxidants; D002374:Catalase; D002934:Cinnamates; D053630:Depsides; D013482:Superoxide Dismutase; D000097123:Rosmarinic Acid