title: Stemness gene expression profile of human adipose derived stem cells in long-term culture.
abstract: It is crucial to know whether stem cells retain its stemnness properties after advance in vitro manipulation. The objective of this study was to investigate the stemness gene expression of human adipose tissue derived stem cells (ADSCs) in long-term culture using quantitative RT-PCR technique. Our data showed that the expression level of Sox-2, Rex-1, FGF-4, Nanog, Nestin, BST-1, FZD-9 and Oct-4 were decreased gradually in long-term culture. This could mean that the ability of ADSCs to differentiate into other cell lineages reduce after extensive culture.
authors.affiliation: Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
authors.forename: W S Wan Kamarul
authors.initials: WS
authors.lastname: Zaman
authors.forename: S
authors.initials: S
authors.lastname: Makpol
authors.forename: S
authors.initials: S
authors.lastname: Santhapan
authors.forename: K H
authors.initials: KH
authors.lastname: Chua
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050154:Adiposity / Q000235:genetics*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005784:Gene Amplification; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors