Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins.
Bordetella pertussis is the causative agent of highly communicable respiratory infection whooping cough (pertussis) which remains one of the world's leading causes of vaccine-preventable deaths. In the present study, total soluble proteins extracted from two B. pertussis strains, Tohama I and the local isolate Saadet were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the antisera obtained from the mice immunized with inactivated whole cells as well as those collected from the mice challenged intraperitoneally with live cells of each strain. Of a total of 25 immunogenic proteins identified, 21 were shown to be the novel antigens for B. pertussis.
Altindiş
Emrah
E
Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey.
Tefon
Burcu E
BE
Yildirim
Volkan
V
Ozcengiz
Erkan
E
Becher
Dörte
D
Hecker
Michael
M
Ozcengiz
Gülay
G
D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D001886:Bordetella pertussis / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D021382:Protein Sorting Signals; D020543:Proteome; D040901:Proteomics / Q000379:methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D020543:Proteome