Production of o-diphenols by immobilized mushroom tyrosinase.
The o-diphenols 4-tert-butyl-catechol, 4-methyl-catechol, 4-methoxy-catechol, 3,4-dihydroxyphenylpropionic acid and 3,4-dihydroxyphenylacetic acid were produced from the corresponding monophenols (4-tert-butyl-phenol, 4-methyl-phenol, 4-methoxy-phenol, p-hydroxyphenylpropionic acid and p-hydroxyphenylacetic acid) using immobilized mushroom tyrosinase from Agaricus bisporus. In all cases the yield was R(diphenol)> or =88-96%, which, according to the literature, is the highest yield so far, obtained using tyrosinase. The reaction was carried out in 0.5M borate buffer pH 9.0 which was used to minimize the diphenolase activity of tyrosinase by complexing the o-diphenols generated. Hydroxylamine and ascorbic acid were also present in the reaction medium, the former being used to reduce mettyrosinase to deoxytyrosinase, closing the catalytic cycle, and the latter to reduce the o-quinone produced to o-diphenol. Inactivation of the tyrosinase by ascorbic acid was also minimized due to the formation of an ascorbic acid-borate complex. Concentrations of the o-diphenolic compounds obtained at several reaction times were determined by gas chromatography-mass spectrometry (GC-MS) and UV-vis spectroscopy. The experimental results are discussed.
Marín-Zamora
María Elisa
ME
Grupo de Química de Carbohidratos y Biotecnología de Alimentos (QCBA). Departamento de Química Orgánica, Facultad de Química, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
Rojas-Melgarejo
Francisco
F
García-Cánovas
Francisco
F
García-Ruiz
Pedro Antonio
PA
D000364:Agaricus / Q000201:enzymology*; D001205:Ascorbic Acid / Q000378:metabolism; D001881:Borates / Q000378:metabolism; D002396:Catechols / Q000032:analysis / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D019811:Hydroxylamine / Q000378:metabolism; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010636:Phenols / Q000032:analysis / Q000378:metabolism*
D001881:Borates; D002396:Catechols; D004800:Enzymes, Immobilized; D010636:Phenols; D019811:Hydroxylamine; D014442:Monophenol Monooxygenase; D001205:Ascorbic Acid