title: An evaluation of a method for the detection of sensory ganglia in product derived from advanced meat recovery systems.
abstract: A method is described for the identification of dorsal root ganglia (DRG)-associated sensory neurons within advanced meat recovery (AMR) product derived from bovine vertebral columns. This method relies on the unique microanatomy of sensory neurons and immunohistochemical staining, primarily for glial fibrillary acidic protein. Sensory neurons are variably sized unipolar neurons, exhibiting a single-cell process that is rarely seen in histologic sections. These neurons are surrounded by a prominent ring of glial fibrillary acidic protein-positive satellite cells that produce a distinctive and readily identifiable staining pattern in histologic sections. Fragmented DRG were detected to the 0.25% level in samples of ground beef or nonvertebral-origin AMR product spiked with these sensory ganglia. Similarly examined commercially produced nonvertebral-origin AMR product (n = 157) did not contain sensory ganglia, while 3.3% of vertebral-origin AMR product (n = 364) contained fragmented DRG.
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, Office of Public Health Science, Athens, Georgia 30604, USA. Scott.Hafner@FSIS.USDA.gov
authors.forename: Scott
authors.initials: S
authors.lastname: Hafner
authors.forename: Mary T
authors.initials: MT
authors.lastname: Sutton
authors.forename: Joseph
authors.initials: J
authors.lastname: Hill
authors.forename: Patrick C
authors.initials: PC
authors.lastname: McCaskey
authors.forename: Lynda Collins
authors.initials: LC
authors.lastname: Kelley
mesh_terms: D000818:Animals; D002417:Cattle; D003257:Consumer Product Safety; D005069:Evaluation Studies as Topic; D005506:Food Contamination / Q000032:analysis*; D005727:Ganglia, Spinal / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis; D015203:Reproducibility of Results; D027161:Satellite Cells, Perineuronal; D012680:Sensitivity and Specificity; D011984:Sensory Receptor Cells
chemical_list: D005904:Glial Fibrillary Acidic Protein