title: Effects of contaminated sediment from Cork Harbour, Ireland on the cytochrome P450 system of turbot.
abstract: Hatchery-reared juvenile turbot (Scophthalmus maximus L.) were exposed for 3 weeks, under laboratory conditions, to inter-tidal sediments collected from polluted sites in Cork Harbour (Whitegate and Agahda) and a reference site at Ballymacoda Co., Cork, Ireland. The potential of the sediment exposure to induce cytochrome P450 activities and CYP1A1 in the fish was assessed. Chemical analysis revealed that the sediments originating from the reference and harbour sites were contaminated principally with PAHs-the harbour sites having double the levels of those at the reference site. Following 3 weeks exposure to the sediments western blotting demonstrated a strong immunogenic response for CYP1A1 in the liver, but not for gill or intestine. P450 activities were generally significantly higher than those exposed to reference site sediment. Liver was the most responsive tissue with significantly greater P450 activities compared with gill and intestinal tissues.
authors.affiliation: Department of Zoology, Ecology and Plant Science, University College Cork, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Kilemade
authors.forename: M G J
authors.initials: MG
authors.lastname: Hartl
authors.forename: J
authors.initials: J
authors.lastname: O'Halloran
authors.forename: N M
authors.initials: NM
authors.lastname: O'Brien
authors.forename: D
authors.initials: D
authors.lastname: Sheehan
authors.forename: C
authors.initials: C
authors.lastname: Mothersill
authors.forename: F N A M
authors.initials: FN
authors.lastname: van Pelt
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000187:drug effects*; D004784:Environmental Monitoring; D004790:Enzyme Induction / Q000187:drug effects; D005412:Flatfishes / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry*; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D007422:Intestines / Q000187:drug effects / Q000201:enzymology; D007494:Ireland; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D012623:Seawater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1