A high-throughput multiplex method adapted for GMO detection.
A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detection of up to 48 short DNA sequences (approximately 70 bp; "signature sequences") from taxa endogenous reference genes, from GMO constructions, screening targets, construct-specific, and event-specific targets, and finally from donor organisms. This assay avoids certain shortcomings of multiplex PCR-based methods already in widespread use for GMO detection. The assay demonstrated high specificity and sensitivity. The results suggest that this assay is reliable, flexible, and cost- and time-effective for high-throughput GMO detection.
Chaouachi
Maher
M
CEA/Institut de Genomique/Centre National de Genotypage/INRA UR EPGV, 2 rue Gaston Cremieux, CP 5724, 91057 Evry cedex, France.
Chupeau
Gaëlle
G
Berard
Aurélie
A
McKhann
Heather
H
Romaniuk
Marcel
M
Giancola
Sandra
S
Laval
Valérie
V
Bertheau
Yves
Y
Brunel
Dominique
D
D005838:Genotype; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide