The mechanism of inhibition of metastasis by cartilage polysaccharide in breast-cancer cells.
As large amounts of porcine cartilage are discarded as waste in daily life, it is necessary to find new uses for them. We extracted polysaccharide from cartilage and performed in vitro and in vivo experiments in cancer cells. A mouse breast-cancer pulmonary metastasis model was set up, and we tried to determine the mechanism of the inhibition of metastasis by cartilage PS (polysaccharide). Effects on tumour size and the progression of metastasis indicated that cartilage PS can obviously inhibit metastasis in breast-cancer cells. The levels of LNR1 (laminin receptor 1), alphavbeta3 integrin and MMP-9 (matrix metalloproteinase-9) in mice treated or not with cartilage PS showed significant differences. Cartilage PS inhibited the growth of MCF-7 human breast adenocarcinoma cells, but had little effect on normal cells. Cartilage PS can inhibit the activity of the MMP-2 and the MMP-9 by decreasing the levels of LNR1 and alphavbeta3 integrin to inhibit metastasis further. In summary, we conclude that cartilage PS can act as a specific anti-metastatic agent in breast-cancer cells.
Liu
An-jun
AJ
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
Hu
Yan-xun
YX
Liu
Chang-jin
CJ
Yao
Xiu-ling
XL
Zhang
Guo-rong
GR
D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D002356:Cartilage / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000187:drug effects / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000556:secondary*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D009362:Neoplasm Metastasis / Q000188:drug therapy*; D009368:Neoplasm Transplantation; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D017474:Receptors, Laminin / Q000187:drug effects / Q000378:metabolism; D012269:Ribosomal Proteins; D013552:Swine
D039302:Integrin alphaVbeta3; D061965:Matrix Metalloproteinase Inhibitors; D011134:Polysaccharides; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9