[Expression of nuclear export factor CRM1 and p27 in glioma].
OBJECTIVE
To investigate the expression of nuclear export factor CRM1, Ser10-phosphorylated p27 and p27 in human gliomas.


METHODS
The expression of CRM1, Ser10-phosphorylated p27 and p27 were investigated in 70 cases of human gliomas and 10 specimens of the normal brain tissue by immunohistochemical technique and Western blot.


RESULTS
There were significant differences on the expression levels of CRM1, Ser10-phosphorylated p27 and p27 among normal brain tissue, gliomas of grades II and gliomas of grades III plus IV (P < 0.01). The expression of CRM1 in gliomas was inversely correlated with the expression of p27 (r(s) = -0.727, P < 0.01) and positively correlated with the expression of Ser10-phosphorylated p27 (r(s) = 0.954, P < 0.01) and Ki-67 (r(s) = 0.799, P < 0.01). Moreover, the expression of Ser10-phosphorylated p27 was inversely correlated with p27 (r(s) = -0.744, P < 0.01) and positively correlated with Ki-67 (r(s) = 0.785, P < 0.01).


CONCLUSIONS
CRM1, through recognizing and binding with Ser10-phosphorylated p27, may promote moving of p27CRM1 from its original locating sites; act as a critical signaling component in the proliferative process of glioma cells and then, plays an important role in the development of gliomas.
Wang
Dong-Lin
DL
Department of Pathology, Medical College of Nantong University, Nantong 226001, China. donglin@ntu.edu.cn
Wang
Yu-Chan
YC
Lu
Peng
P
E
Qun
Q
Shi
Gong-Sheng
GS
D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D002648:Child; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D049790:Nuclear Export Signals / Q000235:genetics; D010766:Phosphorylation; D011379:Prognosis; D055815:Young Adult
D049790:Nuclear Export Signals; D050760:Cyclin-Dependent Kinase Inhibitor p27