title: Discrimination between biothreat agents and 'near neighbor' species using a resequencing array.
abstract: Timely identification of biothreat organisms from large numbers of clinical or environmental samples in potential outbreak or attack scenario is critical for effective diagnosis and treatment. This study aims to evaluate the potential of resequencing arrays for this purpose. Albeit suboptimal, this report demonstrated that respiratory pathogen microarray version 1 can identify Bacillus anthracis, Francisella tularensis, Yersinia pestis and distinguish them from benign 'near neighbor' species in a single assay. Additionally, the sequence information can discriminate strains and possibly the sources of the strains. With further development, it is possible to use resequencing microarrays for biothreat surveillance.
authors.affiliation: Center for Bio/Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA.
authors.forename: Chris R
authors.initials: CR
authors.lastname: Taitt
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Malanoski
authors.forename: Baochuan
authors.initials: B
authors.lastname: Lin
authors.forename: David A
authors.initials: DA
authors.lastname: Stenger
authors.forename: Frances S
authors.initials: FS
authors.lastname: Ligler
authors.forename: Anne W
authors.initials: AW
authors.lastname: Kusterbeck
authors.forename: George P
authors.initials: GP
authors.lastname: Anderson
authors.forename: Sue E
authors.initials: SE
authors.lastname: Harmon
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Shriver-Lake
authors.forename: Steven K
authors.initials: SK
authors.lastname: Pollack
authors.forename: Denise M
authors.initials: DM
authors.lastname: Lennon
authors.forename: Fe
authors.initials: F
authors.lastname: Lobo-Menendez
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Joel M
authors.initials: JM
authors.lastname: Schnur
mesh_terms: D000881:Anthrax / Q000175:diagnosis / Q000382:microbiology; D001408:Bacillus anthracis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D023482:Bioterrorism / Q000517:prevention & control*; D004311:Double-Blind Method; D005603:Francisella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005604:Francisella tularensis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010930:Plague / Q000175:diagnosis / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity; D014406:Tularemia / Q000175:diagnosis / Q000382:microbiology; D015007:Yersinia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015010:Yersinia pestis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification