title: Stimulative effects of light and a temperature downshift on transcriptional expressions of developmentally regulated genes in the initial stages of fruiting-body formation of the basidiomycetous mushroom Lentinula edodes.
abstract: Quantitative reverse transcription PCR experiments were done to analyze the effects of light and a temperature downshift on transcriptional expressions of a variety of developmentally regulated genes in the initial stages of fruiting-body formation of Lentinula edodes wild-type strain FMC2. It was revealed that both proper light conditions and a temperature downshift are required for high levels of transcriptions of the genes and the formation of fruiting bodies. In L. edodes MIL-LEW-M13-1 strain, which has been reported previously to be capable of forming fruiting bodies without a low-temperature treatment, the expressions of the developmentally regulated genes were enhanced by light exposure.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. nakazawa.t.ac@m.titech.ac.jp
authors.forename: Takehito
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Kaneko
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shishido
mesh_terms: D048690:Fruiting Bodies, Fungal / Q000254:growth & development* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008027:Light; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020942:Shiitake Mushrooms / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins