Enhanced production of 1,2-propanediol by tpi1 deletion in Saccharomyces cerevisiae.
Saccharomyces cerevisiae was metabolically engineered to improve 1,2-propanediol production. Deletion of the tpi1 (triosephosphate isomerase) gene in S. cerevisiae increased the carbon flux to DHAP (dihydroxylacetone phosphate) in glycolysis, resulting in increased glycerol production. Then, the mgs and gldA genes, the products of which convert DHAP to 1,2-propanediol, were introduced to the tpi1-deficient strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of the mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both the mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in flask culture.
Jung
Joon-Young
JY
Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Korea.
Choi
Eun-Sil
ES
Oh
Min-Kyu
MK
D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D006019:Glycolysis; D019946:Propylene Glycol / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014305:Triose-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D019946:Propylene Glycol; D011765:Pyruvaldehyde; D014305:Triose-Phosphate Isomerase; D005990:Glycerol