Acrolein toxicity: Comparison with reactive oxygen species.
The toxicity of acrolein was compared with that of reactive oxygen species using a mouse mammary carcinoma FM3A cell culture system. Complete inhibition of cell growth was accomplished with 10 microM acrolein, 100 microM H(2)O(2), and 20 microM H(2)O(2) plus 1mM vitamin C, which produce ()OH, suggesting that toxicity of acrolein is more severe than H(2)O(2) and nearly equal to that of ()OH, when these compounds were added extracellularly. Acrolein toxicity was prevented by N-acetyl-l-cysteine and N-benzylhydroxylamine, and attenuated by putrescine and spermidine. Toxicity of H(2)O(2) was prevented by glutathione peroxidase plus N-acetyl-l-cysteine, pyruvate, catalase, and reduced by polyphenol, and toxicity of ()OH was prevented by glutathione peroxidase plus N-acetyl-l-cysteine, pyruvate, catalase and reduced by N-acetyl-l-cysteine. The results indicate that prevention of cell toxicity by N-acetyl-l-cysteine was more effective with acrolein than with ()OH. Protein and DNA synthesis was damaged primarily by acrolein and reactive oxygen species, respectively.
Yoshida
Madoka
M
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
Tomitori
Hideyuki
H
Machi
Yoshiki
Y
Hagihara
Motofumi
M
Higashi
Kyohei
K
Goda
Hitomi
H
Ohya
Takeshi
T
Niitsu
Masaru
M
Kashiwagi
Keiko
K
Igarashi
Kazuei
K
D000111:Acetylcysteine / Q000494:pharmacology; D000171:Acrolein / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D006898:Hydroxylamines / Q000494:pharmacology; D046911:Macromolecular Substances / Q000037:antagonists & inhibitors / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D014176:Protein Biosynthesis / Q000187:drug effects; D011700:Putrescine / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000633:toxicity*; D026082:Singlet Oxygen / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology
D006898:Hydroxylamines; D046911:Macromolecular Substances; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; C017064:benzyloxyamine; D000171:Acrolein; D006861:Hydrogen Peroxide; D013095:Spermidine; D011700:Putrescine; D000111:Acetylcysteine