title: Combining subproteome enrichment and Rubisco depletion enables identification of low abundance proteins differentially regulated during plant defense.
abstract: Transgenic Arabidopsis conditionally expressing the bacterial avrRpm1 type III effector under the control of a dexamethasone-responsive promoter were used for proteomics studies. This model system permits study of an individual effector without interference from additional bacterial components. Coupling of different prefractionation approaches to high resolution 2-DE facilitated the discovery of low abundance proteins - enabling the identification of proteins that have escaped detection in similar experiments. A total of 34 differentially regulated protein spots were identified. Four of these (a remorin, a protein phosphatase 2C (PP2C), an RNA-binding protein, and a C2-domain-containing protein) are potentially early signaling components in the interaction between AvrRpm1 and the cognate disease resistance gene product, resistance to Pseudomonas syringae pv. maculicola 1 (RPM1). For the remorin and RNA-binding protein, involvement of PTM and post-transcriptional regulation are implicated, respectively.
authors.affiliation: Leibniz Institute of Plant Biochemistry, Halle, Germany.
authors.forename: Ivy
authors.initials: I
authors.lastname: Widjaja
authors.forename: Kai
authors.initials: K
authors.lastname: Naumann
authors.forename: Udo
authors.initials: U
authors.lastname: Roth
authors.forename: Noreen
authors.initials: N
authors.lastname: Wolf
authors.forename: David
authors.initials: D
authors.lastname: Mackey
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Dangl
authors.forename: Dierk
authors.initials: D
authors.lastname: Scheel
authors.forename: Justin
authors.initials: J
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000276:immunology; D029681:Arabidopsis Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate; D008861:Microsomes / Q000737:chemistry; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D030821:Plants, Genetically Modified; D040901:Proteomics / Q000379:methods*; D044224:Pseudomonas syringae / Q000502:physiology; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry
chemical_list: D029681:Arabidopsis Proteins; C502082:AvrRpm1 protein, Pseudomonas syringae; D001426:Bacterial Proteins; C095014:RPM1 protein, Arabidopsis; D012273:Ribulose-Bisphosphate Carboxylase