(S)-2-amino-6-nitrohexanoic acid binds to human arginase I through multiple nitro-metal coordination interactions in the binuclear manganese cluster.
The binding affinity of (S)-2-amino-6-nitrohexanoic acid to human arginase I was studied using surface plasmon resonance (K(d) = 60 microM), and the X-ray crystal structure of the enzyme-inhibitor complex was determined at 1.6 A resolution to reveal multiple nitro-metal coordination interactions.
Zakharian
Tatiana Y
TY
Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Di Costanzo
Luigi
L
Christianson
David W
DW
D000614:Aminocaproates; D001119:Arginase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry; D055592:Biophysical Phenomena; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology; D002625:Chemistry, Organic / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008345:Manganese / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D008956:Models, Chemical; D015394:Molecular Structure; D009574:Nitro Compounds / Q000737:chemistry* / Q000494:pharmacology; D009584:Nitrogen / Q000737:chemistry*; D011485:Protein Binding
C542346:(S)-2-amino-6-nitrohexanoic acid; D000614:Aminocaproates; D002208:Caproates; D008024:Ligands; D008670:Metals; D009574:Nitro Compounds; C037652:hexanoic acid; D008345:Manganese; D001120:Arginine; D001119:Arginase; D009584:Nitrogen