Molecular typing and colonization patterns of Aspergillus fumigatus in patients with cystic fibrosis.
Aspergillus fumigatus is a chronic colonizer of the respiratory tract of patients with cystic fibrosis (CF). A total of 204 A. fumigatus isolates from 36 CF patients from three different medical centers, collected over a period of four months till 9.5 years, were genotyped using the short tandem repeat panel for A. fumigatus (STRAf assay). Four different colonization patterns were observed. Colonization patterns with only unique genotypes were found in 36% of the patients. In contrast 17% of the patients were chronically colonized with a single genotype. The remaining patients showed a predominant genotype or genotypes that succeed each other. In this collection no relation was found between colonization patterns and allergic bronchopulmonary aspergillosis.
de Valk
Hanneke A
HA
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands. h.v.haren@cwz.nl
Klaassen
Corné H W
CH
Yntema
Jan-Bart
JB
Hebestreit
Alexandra
A
Seidler
Marc
M
Haase
Gerhard
G
Müller
Frank-Michael
FM
Meis
Jacques F G M
JF
D001232:Aspergillus fumigatus / Q000235:genetics* / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D007223:Infant; D016533:Mycological Typing Techniques; D012137:Respiratory System / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology