[Role of secondary lymphoid tissue chemokine in the pathogenesis of rat ulcerative colitis].
OBJECTIVE
To investigate the effect of secondary lymphoid tissue chemokine (SLC) on experimental colon lesions in rats with ulcerative colitis.


METHODS
Sixty Sprague-Dawley rats were randomly divided into control group, model group and SLC intervention group. Colonic mucosal lesions of different groups were observed with HE staining for inflammation and lymphocyte homing situation. Cytokine IL-2 and IL-6 levels were measured by ABC-ELISA. Semi-quantitative RT-PCR was used to examine the colonic SLC expression.


RESULTS
Intestinal inflammation score and colonic cytokine levels were significantly different among three groups (P<0.05, P<0.01). Abnormal lymphocyte homing phenomenon under colonic mucosa was found in the model group and the intervention group. SLC mRNA expression of the model and intervention groups increased significantly compared with the control group (0.846+/-0.047, 0.768+/-0.135 vs 0.312+/-0.112, P<0.01). However, there was no significant difference between model group and intervention group.


CONCLUSIONS
SLC may play an important role in experimental colonic mucosal inflammation in rats with ulcerative colitis. Blockade of SLC may be one of effective ways in reducing colonic mucosal inflammation.
Ge
Bu-jun
BJ
Department of Surgery, Tongji Hospital, Tongji University, Shanghai 200065, China. gebujun@126.com
Chen
Xi-mei
XM
Yang
Chang-qing
CQ
Wu
Jian
J
D000818:Animals; D054421:Chemokine CCL21 / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D007249:Inflammation; D007376:Interleukin-2 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D054421:Chemokine CCL21; D007376:Interleukin-2; D015850:Interleukin-6