title: In vitro growth of human umbilical blood mesenchymal stem cells and their differentiation into chondrocytes and osteoblasts.
abstract: Conditions for culturing and differentiation of human umbilical blood mononuclear cells in vitro were studied. The growth of mesenchymal stem cells was attained in 31 of 54 (57.4%) umbilical blood samples and morphological and immunophenotypical authenticity of these cells was confirmed. Stimulatory effects of 20% AB(IV) human serum and transforming growth factor-beta (TGF-beta) on the growth of mesenchymal stem cells were demonstrated. Osteogenic cells formed in the presence of differentiation factors ascorbic acid, dexamethasone, and beta-glycerophosphate, while chondrogenic cells developed in the presence of dexamethasone, ascorbic acid, and TGF-beta. Differentiation of mesenchymal stem cells was confirmed by histochemical and molecular genetic tests.
authors.affiliation: National Center of Hematology and Transfusiology, Minsk.
authors.forename: S M
authors.initials: SM
authors.lastname: Kosmacheva
authors.forename: M V
authors.initials: MV
authors.lastname: Volk
authors.forename: T A
authors.initials: TA
authors.lastname: Yeustratenka
authors.forename: I N
authors.initials: IN
authors.lastname: Severin
authors.forename: M P
authors.initials: MP
authors.lastname: Potapnev
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D048430:Cell Shape; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D020219:Chondrogenesis / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology