[The study on differential gene expression profiling in pulmonary tissue of rats exposed to silica early].
OBJECTIVE
To study the differential gene expression profiling of rats exposed to silica using the normal rats as control.


METHODS
Animal models were established using intratracheal injection of the lung and 22 107 genes were screened in the differential expression profiling of silicosis by using oligonucleotide bead array. Differential expression profiling data were analyzed by using DAVID bioinformation software.


RESULTS
Totally 1567 differentially expressed genes were identified in lungs of silica exposed rats including 765 up-regulated genes and 802 down-regulated genes as compared to the normal controls. Among 406 annotated genes in KEGG pathways, 204 genes and 11 pathways were up-regulated as well as 202 genes and 3 pathways were down-regulated in silica exposed rats.


CONCLUSIONS
All 1567 genes are involved in the formation of silicosis. The differential gene expression profile of silicosis describes the general changes in the gene expressions in silicosis at transcriptional level. Further analysis of the identified genes might help reveal the molecular mechanism of pulmonary fibrosis induced by silica.
Chen
Lei
L
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.
Wang
Shi-Xin
SX
Liu
Ping
P
Wei
Mao-Ti
MT
Luan
Da-Wei
DW
He
Rui-Bo
RB
Tu
Zhi-Guang
ZG
D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011658:Pulmonary Fibrosis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012829:Silicosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology