title: Diallyl disulfide (DADS) induces apoptosis in human cervical cancer Ca Ski cells via reactive oxygen species and Ca2+-dependent mitochondria-dependent pathway.
abstract: The mechanisms of apoptosis induced by diallyl disulfide (DADS) were explored in human cervical cancer Ca Ski cells. Flow cytometric analysis, DNA gel electrophoresis and DAPI staining demonstrated that DADS induced apoptosis in Ca Ski cells. DADS induced apoptosis through the production of reactive oxygen species and Ca2+, and induced abrogation of mitochondrial membrane potential (Deltapsim) and cleavage of Bid protein (t-Bid). DADS increased the levels of p53, p21 and Bax, but caused a decrease in the level of Bcl-2. DADS also promoted the activities of caspase-3 leading to DNA fragmentation, thus indicating that DADS-induced apoptosis is caspase-3 dependent. In addition, DADS induced an increase in the level of cytochrome c in the cytoplasm, which was released from mitochondria. BAPTA attenuated the Deltapsim abrogation and significantly diminished the occurrence of DADS-induced apoptosis in Ca Ski cells. In conclusion, DADS-induced apoptosis occurs via production of ROS and caspase-3 and a mitochondria-dependent pathway in Ca Ski cells.
authors.affiliation: Department of Nursing and Management, Jen-Teh Junior College of Medicine, Miaoli, Taiwan, R.O.C.
authors.forename: Yuh-Tzy
authors.initials: YT
authors.lastname: Lin
authors.forename: Jai-Sing
authors.initials: JS
authors.lastname: Yang
authors.forename: Shuw-Yuan
authors.initials: SY
authors.lastname: Lin
authors.forename: Tzu-Wei
authors.initials: TW
authors.lastname: Tan
authors.forename: Chin-Chin
authors.initials: CC
authors.lastname: Ho
authors.forename: Te-Chun
authors.initials: TC
authors.lastname: Hsia
authors.forename: Tsan-Hung
authors.initials: TH
authors.lastname: Chiu
authors.forename: Chun-Shu
authors.initials: CS
authors.lastname: Yu
authors.forename: Hsu-Feng
authors.initials: HF
authors.lastname: Lu
authors.forename: Yueh-Shan
authors.initials: YS
authors.lastname: Weng
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D004220:Disulfides / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000498:Allyl Compounds; D000970:Antineoplastic Agents; D004220:Disulfides; D017382:Reactive Oxygen Species; C028009:diallyl disulfide; D053148:Caspase 3; D002118:Calcium