Effects of different media on the in vivo chondrogenesis of sheep bone marrow stem cells: histological assessment.
This study aimed to compare the effects of three different media on the in vivo chondrogenesis of sheep bone marrow stem cells (BMSC). Sheep BMSC were cultured in F12:DMEM + 10% FBS, chondrogenic medium containing 5ng/ml TGF,3 + 50ng/ml IGF-1 and UKM-MECC for three weeks. The cultured cells were then harvested for construct formation with fibrin. Constructed tissues were implanted subcutaneously into nude mice for in vivo development. Cell aggregates were formed in both chondrogenic medium and UKM-MECC demonstrated the early chondrogenesis process. After five weeks of in vivo development, both chondrogenic medium and UKM-MECC promoted cartilage matrix synthesis confirmed by Safranin O staining.
Alfaqeh
H
H
Department of Physiology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
Chua
K H
KH
Aminuddin
B S
BS
Ruszymah
B H I
BH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002449:Cell Aggregation; D018929:Cell Culture Techniques; D019902:Chondrocytes; D020219:Chondrogenesis / Q000502:physiology*; D003470:Culture Media; D006801:Humans; D023421:Models, Animal; D012756:Sheep
D003470:Culture Media