[Multiparametric flow cytometry analyzes the expressions of immunophenotype CD133, CD34, CD44 in lung cancer naive cells].
OBJECTIVE
To study the expression of CD133, CD34 and CD44 in lung cancer, and distribution of cell subpopulations that generated by combination of different markers.


METHODS
By collecting 10 normal lung tissues and 40 lung cancer fresh samples that were diagnosed pathologically, preparing single cell suspension, and labelling cells with CD133, CD34, CD44, CD117, CD43, CD45 and LIN(CD2, CD3, CD31, CD64) monoclone antibodies, we analyzed the distribution of the above-mentioned antibodies in lung cancer cells on FCM. By double-parameters plots, we analyzed the naive cell subpopulations that were generated through combination of positive and negative antigen expression.


RESULTS
In lung cancer parenchymal-cells (LIN- CD45-) expressed CD133, CD34 and CD44 dim positive and, expressed CD117 and CD43 negative. The mann-Whitney rank test results shows that there was no significant difference for the expression of CD133, CD34, CD44 between lung cancer and normal lung tissue, and CD44+, CD133- CD34-, CD133+ CD44+, CD133+ CD44-, CD133+ CD34+, CD133- CD34+ and CD133- CD44+ expressed differently (P < 0.05) between lung adenocarcinoma and lung squamous cell carcinoma. The cluster analysis results suggest that they are classified to 4 subsets.


CONCLUSIONS
CD133, CD34, CD44 could be the marker of lung cancer naive cells, which could contribute to study lung cancer cell development.
Liu
Dan
D
Department of Respiratory Medicine, Laboratory Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Suchian University, Chengdu, China.
Li
Wei-min
WM
Mo
Xian-ming
XM
Liu
Lun-xu
LX
Wang
Yun
Y
Che
Guo-wei
GW
Wu
Zhu
Z
Gou
Ji-long
JL
D000071916:AC133 Antigen; D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology; D015703:Antigens, CD / Q000096:biosynthesis*; D018952:Antigens, CD34 / Q000096:biosynthesis*; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D016130:Immunophenotyping; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008297:Male; D014411:Neoplastic Stem Cells / Q000276:immunology*; D010455:Peptides
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D018960:Hyaluronan Receptors; C000606023:PROM1 protein, human; D010455:Peptides