Identification and physiological evaluation of the components from citrus fruits as potential drugs for anti-corpulence and anticancer.
On the basis of monitoring the prevention of accumulation of lipid droplets in mouse 3T3-L1 preadipocyte cells and inhibition of the proliferation of human colon cancer HT-29 cells, effective anti-corpulence and anticancer compounds were isolated from the peel of Citrus fruits. These bioactive components were identified as polymethoxyflavones and coumarin derivatives by spectroscopic analyses. 5-Hydroxy-6,7,8,3',4'-pentamethoxyflavone had the greatest anti-corpulence effects and 3,5,6,7,8,3',5'-heptamethoxyflavone had the greatest anticancer effects. Furthermore, distributions of those bioactive components in the peel of 10 species of Citrus fruits were demonstrated by HPLC analyses.
Hirata
Toshifumi
T
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Japan. hirata-001@hiroshima-u.ac.jp
Fujii
Misato
M
Akita
Kazuhiro
K
Yanaka
Noriyuki
N
Ogawa
Kaori
K
Kuroyanagi
Masanori
M
Hongo
Daiki
D
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002957:Citrus / Q000737:chemistry*; D003374:Coumarins / Q000302:isolation & purification* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D047309:Flavones / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D051379:Mice
D019440:Anti-Obesity Agents; D000970:Antineoplastic Agents; D003374:Coumarins; D047309:Flavones; C030123:coumarin