Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae).
A microsporidium possessing molecular and morphological characteristics of the genus Nosema was isolated from larvae of the thee-spot grass yellow butterfly, Eurema blanda arsakia. The complete rRNA gene sequences of the E. blanda isolate contained 4,428 base pairs (GenBank Accession No. EU338534). The organization of the rRNA genes is LSU rRNA-ITS-SSU rRNA-IGS-5S, which corresponds with that of Nosema species closely related to Nosema bombycis. Phylogenetic analysis based on rRNA gene sequences show that this isolate is closely related to Nosema bombycis, Nosema plutellae, Nosema spodopterae, and Nosema antheraeae. The ultrastructure of all developmental stages of this microsporidium confirmed its placement in the genus Nosema. The isolate was successfully propagated in cell lines IPLB-LD652Y (Lymantria dispar) and NTU-LY (Lymantria xylina) and, in the in vitro system, it was frequently found to develop in the nuclei of the host cells, a circumstance that seldom occurs in other Nosema species. An extra-cellular vegetative stage of this microsporidium was also observed in the culture medium after 14 days of infection. The ECMDFs might be released from disrupted host cells.
Tsai
Yi-Chun
YC
Department of Entomology, College of Bioresources and Agriculture, National Taiwan University, Taipei 106, Taiwan, ROC.
Solter
Leellen F
LF
Wang
Chih-Yuan
CY
Fan
Huai-Sheng
HS
Chang
Chun-che
CC
Wang
Chung-Hsiung
CH
D000818:Animals; D002080:Butterflies / Q000382:microbiology*; D002460:Cell Line; D003470:Culture Media; D005819:Genetic Markers; D007814:Larva / Q000382:microbiology; D046529:Microscopy, Electron, Transmission; D009172:Mycology / Q000379:methods; D016818:Nosema / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure
D003470:Culture Media; D005819:Genetic Markers; D012335:RNA, Ribosomal