A fibrin gel carrier system for islet transplantation into kidney subcapsule.
Islet transplantation is a promising therapeutic option for type 1 diabetes, and actively performed in the clinic as well as in the animal experiments. For the rodent experiments, islet transplantation into kidney subcapsule is widely used to assess islet quality, however, it is often difficult to do using a polyethylene tubing and fine needle because of inherent dead volume of needle and stickiness of the tubing to islets. This problem makes it difficult to interpret the physiological response to different islet doses. Here, we developed a simple fibrin gel carrier system for islet transplantation into kidney subcapsule and utilized it to determine the marginal islet mass sufficient for correction of hyperglycemia in diabetic nude mice.
Lim
Jong-Yeon
JY
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea.
Min
Byoung-Hoon
BH
Kim
Byoung-Geun
BG
Han
Hyun-Ju
HJ
Kim
Soo-Jin
SJ
Kim
Chan-Wha
CW
Han
Sung-Sik
SS
Shin
Jun-Seop
JS
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000473:pathology / Q000601:surgery; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007668:Kidney / Q000473:pathology / Q000601:surgery*; D051379:Mice; D008819:Mice, Nude; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D015718:Fibrin Tissue Adhesive; D007328:Insulin