[Characterization of RdRp gene of rabies virus in China].
OBJECTIVE
Characterization of RdRp gene (L) of rabies virus aG and CTN181 strain in China.


METHODS
Overlapped fragments were amplified and assembled. Then characterization and phylogenetic analyses as well as prediction of functional regions were performed using biologic softwares.


RESULTS
L gene of CTN181 and aG strains were composed with 6387nt and 6384nt respectively and there were two repeated ATG in the start of L gene in CTN181 strain. In addition, some mutations and new functional regions were discovered and presumed to be crucial to the role of these regions in replication of rabies virus. Analyses of L gene provide new insight into the functional regions, and phylogenetic analyses showed that human vaccine strain aG were independent to others recommended by WHO.


CONCLUSIONS
The characterization of complete L genes will be helpful for research on new functional regions, antiviral drugs targeted at RdRp and phylogenetic analyses.
Du
Jia-Liang
JL
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhang
Qiang
Q
Tao
Xiao-Yan
XY
Li
Hao
H
Liang
Guo-Dong
GD
Tang
Qing
Q
D002681:China; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011818:Rabies / Q000821:virology*; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; C097625:L protein, Rabies virus; D012321:DNA-Directed RNA Polymerases