title: [An experimental study on percutaneous autologous bone marrow mesenchymal stem cell grafting for bone defect].
abstract: OBJECTIVE
  To investigate in vive osteogenic potential in size-critical bone defect after percutaneous autologous grafting of culture-expanded rabbit autologous BMSCs, osteo-induced BMSCs and combination of both.
  
  
  METHODS
  BMSCs were cultured and then induced with osteogenic supplement (OS) medium. BMSCs with and without OS induction were collected and percutaneously autologously injected respectively into the 15 mm bone defect of 20 experimental rabbit model. The grafts were BMSCs, osteo-induced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, and 0.9% NaCl solution. Osteogenesis at the defect areas were observed by regular radiography, histology and biomechanics.
  
  
  RESULTS
  The group transplanted with BMSCs + osteo-induced BMSCs achieved complete bone healing with medullary cavity united, which showed the largest quantity of new bone measured by X-ray analysis, and also their maximal load were better than those in other groups.
  
  
  CONCLUSIONS
  The bone-forming ability of rabbit osteo-induced BMSCs combined with BMSCs in bone defect is superior to those of BMSCs and osteo-induced BMSCs.
authors.affiliation: Department of Orthopaedics, Beijing Army General Hospital, Beijing 100700, China.
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Chang
authors.forename: Tian-Sheng
authors.initials: TS
authors.lastname: Sun
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zhang
authors.forename: Zhou-Yong
authors.initials: ZY
authors.lastname: Ma
authors.forename: Qiang
authors.initials: Q
authors.lastname: Su
authors.forename: Da-Jiang
authors.initials: DJ
authors.lastname: Ren
authors.forename: Li-Ren
authors.initials: LR
authors.lastname: Zhang
authors.forename: Shao-Ting
authors.initials: ST
authors.lastname: Xu
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000601:surgery*; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011817:Rabbits; D014182:Transplantation, Autologous