Two-dimensional difference gel electrophoresis analysis of Streptococcus uberis in response to mutagenesis-inducing ciprofloxacin challenge.
In Streptococcus uberis, the fluoroquinolone antibiotic ciprofloxacin induces a mutagenic response that is distinct from the SOS paradigm. Two-dimensional differential gel electrophoresis was employed to investigate the effect of ciprofloxacin exposure on the proteome of S. uberis. Twenty-four protein spots exhibiting differential expression (p < 0.05) were identified as enzymes with potential role in oxidative stress, NADH generation and nucleotide biosynthesis. We suggest that these metabolic changes provide S. uberis means to stimulate mutagenesis and adaptation.
Poutanen
Marjo
M
Institute of Biotechnology, and Department of Basic Veterinary Sciences, University of Helsinki, Finland.
Varhimo
Emilia
E
Kalkkinen
Nisse
N
Sukura
Antti
A
Varmanen
Pekka
P
Savijoki
Kirsi
K
D001483:Base Sequence; D002232:Carbocyanines / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009711:Nucleotides / Q000737:chemistry; D018384:Oxidative Stress; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013291:Streptococcus / Q000378:metabolism*
D002232:Carbocyanines; D009711:Nucleotides; C089187:cyanine dye 3; C085321:cyanine dye 5; D002939:Ciprofloxacin