title: Zonal gene expression of chondrocytes in osteoarthritic cartilage.
abstract: OBJECTIVE
  To determine the chondrocyte metabolism in respective zones of osteoarthritic (OA) cartilage.
  
  
  METHODS
  OA cartilage was obtained from macroscopically intact areas of 4 knee joints with end-stage OA. The cartilage was divided into 3 zones, and gene expression profiles were determined in the respective zones by a custom-designed microarray that focused on chondrocyte-related genes. For the genes whose expression was significantly different among the zones, the expression was compared between OA and control cartilage in the respective zones by an analysis using laser capture microdissection and real-time polymerase chain reaction (PCR). For some genes, the correlation of expression was investigated in specific cartilage zones.
  
  
  RESULTS
  A total of 198 genes (approximately 40% of those investigated) were found to be expressed at significantly different levels among the zones. Expression of 26 of those genes was evaluated by laser capture microdissection and real-time PCR, which confirmed the validity of microarray analysis. The expression of cartilage matrix genes was mostly enhanced in OA cartilage, at similar levels across the zones but at different magnitudes among the genes. The expression of bone-related genes was induced either in the superficial zone or in the deep zone, and positive correlations were found among their expression in the respective zones. The expression of 5 proteinase genes was most enhanced in the superficial zone, where their expression was correlated, suggesting the presence of a common regulatory mechanism(s) for their expression.
  
  
  CONCLUSIONS
  In OA cartilage, the metabolic activity of chondrocytes differed considerably among zones. Characteristic changes were observed in the superficial and deep zones.
authors.affiliation: Clinical Research Center, National Hospital Organization Sagamihara Hospital, Kanagawa, Japan. n-fukui@sagamihara-hosp.gr.jp
authors.forename: Naoshi
authors.initials: N
authors.lastname: Fukui
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hikita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mitomi
authors.forename: Nobuho
authors.initials: N
authors.lastname: Tanaka
authors.forename: Yozo
authors.initials: Y
authors.lastname: Katsuragawa
authors.forename: Seizo
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Motoji
authors.initials: M
authors.lastname: Sawabe
authors.forename: Takuo
authors.initials: T
authors.lastname: Juji
authors.forename: Toshihito
authors.initials: T
authors.lastname: Mori
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.forename: Shiro
authors.initials: S
authors.lastname: Ikegawa
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001842:Bone and Bones / Q000502:physiology; D002358:Cartilage, Articular / Q000473:pathology / Q000502:physiology*; D019902:Chondrocytes / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D045726:Metalloproteases / Q000235:genetics; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D020370:Osteoarthritis, Knee / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D045726:Metalloproteases