Analysis of dibenzothiophene desulfurization in a recombinant Pseudomonas putida strain.
Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early exponential growth phases was designed to significantly increase biodesulfurization.
Calzada
Javier
J
Departamento de Ingeniería Química, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Spain.
Zamarro
María T
MT
Alcón
Almudena
A
Santos
Victoria E
VE
Díaz
Eduardo
E
García
José L
JL
Garcia-Ochoa
Felix
F
D001426:Bacterial Proteins / Q000096:biosynthesis; D004798:Enzymes / Q000096:biosynthesis; D020869:Gene Expression Profiling; D016958:Pseudomonas putida / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D001426:Bacterial Proteins; D004798:Enzymes; D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene