[Effects of hnRNP B, siRNA on the expression of p53 in human lung adenocarcinoma cell line A549].
OBJECTIVE
To explore the effects of hnRNP B1 on the expression of p53 in human lung adenocarcinoma cell line A549.


METHODS
Two lines of A549 cells transfected by hnRNP B1 specific siRNA (recombinant plasmid A and D), which had a high transfection efficiency, were established as experimental groups. One line of A549 cells and one line of A549 cells transfected by pure plasmid served as controls. All of the cells were collected after treated with 10 micromol/L cisplatin for 24 h. The expressions of p53 mRNA and protein were detected by real-time RT-PCR and Western blot analysis. The expression of Phospho-P53 protein was detected by western blot analysis.


RESULTS
No significant differences in the expression of p53 mRNA and protein among the groups were found. The expression ratio of Phospho-P53 protein in the cells transfected by hnRNP B1 siRNA were 0.87 +/- 0.06 and 0.75 +/- 0.06, which were significantly greater than those in the A549 cell (0.21 +/- 0. 04) and in the A549 cells transfected by pure plasmid (0.30 +/- 0.08) (P < 0.01).


CONCLUSIONS
hnRNP B, specific siRNA can up regulate the activity of p53 in A549 cells.
Han
Juan
J
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
Li
Wei-min
WM
Tang
Feng-ming
FM
Pu
Dan
D
Chen
Wen-bin
WB
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D016158:Genes, p53 / Q000235:genetics*; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C439768:hnRNP A2