[Refined mapping of heterozygosity loss on chromosome 5p15 in sporadic colorectal cancer].
OBJECTIVE
To refine the loss of heterozygosity (LOH) on chromosome 5p15 and screen new tumor suppressor gene(s) in colorectal tumorigenesis.


METHODS
Samples of colorectal cancer and normal tissue of 83 cases were collected in this study. Sixteen polymorphic microsatellite markers were analyzed on chromosome 5 and another 6 markers on chromosome 5p15 by PCR. PCR products were electrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis.


RESULTS
Two distinct regions of frequent allelic deletions at D5S416 on 5p15 and D5S428-D5S410 on 5q were detected. Another 6 polymorphic microsatellite markers were applied to 5p15 and the minimal region of frequrent loss of heterozygosity was established on 5p15 spanning the D5S416 locus.


CONCLUSIONS
A critical and precise location of 5p deletions, 5p15.2-5p15.3, has been detected, which may contain one or more unknown tumor suppressor gene(s) related to colorectal cancer.
Xu
Shi-feng
SF
Department of General Surgery, the Shandong Provincial Hospital, Jinan, China. xushifengdoctor@sohu.com
Li
Da-peng
DP
Peng
Zhi-hai
ZH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002874:Chromosome Mapping / Q000379:methods; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction