Stabilization and conformational isomerization of the cofactor during the catalysis in hydrolytic ALDHs.
Over the past 15 years, mechanistic and structural aspects were studied extensively for hydrolytic ALDHs. One the most striking feature of nearly all X-ray structures of binary ALDH-NAD(P)(+) complexes is the great conformational flexibility of the NMN moiety of the NAD(P)(+), in particular of the nicotinamide ring. However, the fact that the acylation step is efficient in GAPN (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase) from Streptococcus mutans and in other hydrolytic ALDHs implies an optimal positioning of the nicotinamide ring relative to the hemithioacetal intermediate within the ternary complex to allow an efficient and stereospecific hydride transfer. Another key aspect of the chemical mechanism of this ALDH family is the requirement for the reduced NMN (NMNH) to move away from the initial position of the NMN for adequate positioning and activation of the deacylating water molecule by invariant E268 for completion of the reaction. In recent years, significant efforts have been made to characterize structural and molecular factors involved in the stabilization of the NMN moiety of the cofactor during the acylation step and to provide structural evidence of conformational isomerization of the cofactor during the catalytic cycle of hydrolytic ALDHs. The results presented here will be discussed for their relevance to the two-step catalytic mechanism and from an evolutionary viewpoint.
Talfournier
François
F
Faculté des Sciences et Techniques, UMR 7567 CNRS - Nancy Université, Maturation des ARN et Enzymologie Moléculaire, Vandoeuvre-lès-Nancy Cedex, France. francois.talfournier@maem.uhp-nancy.fr
Pailot
Arnaud
A
Stinès-Chaumeil
Claire
C
Branlant
Guy
G
D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D004795:Enzyme Stability; D006868:Hydrolysis; D007536:Isomerism; D008958:Models, Molecular; D009536:Niacinamide / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
D009536:Niacinamide; D000444:Aldehyde Dehydrogenase