Recurrent chromosomal abnormalities in human embryonic stem cells.
Cultured human embryonic stem (hES) cells have a known predisposition to aneuploidy of chromosomes 12, 17 and X. We studied 17 hES cell lines by array-based comparative genomic hybridization (aCGH) and found that the cells accumulate other recurrent chromosomal abnormalities, including amplification at 20q11.21 and a derivative chromosome 18. These genomic changes have a variable impact at the transcriptional level.
Spits
Claudia
C
Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090, Brussels, Belgium. laudia.spits@uzbrussel.be
Mateizel
Ileana
I
Geens
Mieke
M
Mertzanidou
Afroditi
A
Staessen
Catherine
C
Vandeskelde
Yves
Y
Van der Elst
Josiane
J
Liebaers
Inge
I
Sermon
Karen
K
D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D053595:Embryonic Stem Cells / Q000166:cytology / Q000648:ultrastructure; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data