[A nondestructive method to measure the oxygen binding and releasing properties of biodegradable polymers microcapsules intented for blood substitutes].
P50 is an important parameter reflecting the binding and releasing oxygen properties of blood substitutes. In this study, based on the strong penetrating property of near infrared light and the mechanism involved in the pulsatile oxygen meter in clinic as well as on the ability for penetrating biodegradable polymers and detecting bovine hemoglobin encapsulated within the microcapsules, we have made an airproof and equilibrium apparatus to measure oxygen saturation and oxygen partial pressure. Subsequently, we have obtained the oxygen dissociation curve and P50 of the microcapsules loaded bovine hemoglobin in the light of oxyHemoglobin and deoxyHemoglobin with different spectrum in the near infrared region. The above-mentioned apparatus and method are not destructive to the microcapsules, and the process is simple and nondestructive. So it is practical to take in-situ measurements of the oxygen binding and releasing property of biodegradable polymer microcapsules intented for the blood substitute.
Zhang
Xiaolan
X
Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.
Yuan
Yuan
Y
Wang
Kai
K
Shan
Xiaoqian
X
Zhao
Jian
J
Sheng
Yan
Y
Liu
Changsheng
C
D000818:Animals; D001673:Biodegradation, Environmental; D001802:Blood Substitutes / Q000032:analysis / Q000737:chemistry*; D002214:Capsules; D002417:Cattle; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry
D001802:Blood Substitutes; D002214:Capsules; D006454:Hemoglobins; D010108:Oxyhemoglobins; D011108:Polymers; C100003:deoxyhemoglobin; D010100:Oxygen