[Property of liposomal fusion induced by acid-sensitive polymer].
The fusion between liposome-liposome, liposome-biomembarnes induced by acid-sensitive polymers has been systematically investigated. The polymer-liposomes were constructed by post-insertion method with the poly (2-ethylacrylic acid) (PEAA) alkylamide derivatives. The liposomal fusion was studied by use of fluorescence resonance energy transfer assay, particle size, fluorescent-photometer. The results indicated that the poly (2-ethylacrylic acid)-liposomes has very strong acidic induced fusion capability. Under acidic conditions, acid-sensitive polymer liposomes fused each other, the fusion closely related to the molecular weight of acid sensitivity polymer on the surface of liposomes. The acidic fusion of polymer-liposomes was dependent upon the lipids composition, the degree of fusion was reversely related to the cholesterol contents. Acid-en ci-nsitive polymer liposomes fused with erythrocyte ghosts. The liposomal fusion induced by acid-sensitive polymer associated with the increase of membrane permeability. The good acid-sensitivity of PEAA has been further demonstrated by membrane fusion in current experiments, and the liposomes prepared with lipid anchored-poly (2-ethylacrylic acid) were developeds s a potential pH sensitive delivery system.
Wang
Ru-tao
RT
Faculty of Life Sciences, Northwestern Polytechnical University, Xi' an 710072, China.
Chen
Tao
T
Wang
Zhao
Z
Hui
Min-quan
MQ
Fu
Jing-guo
JG
D000179:Acrylates / Q000737:chemistry*; D000478:Alkylation; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008561:Membrane Fusion; D008970:Molecular Weight; D010316:Particle Size; D011108:Polymers; D013696:Temperature
D000179:Acrylates; D004337:Drug Carriers; D008055:Lipids; D008081:Liposomes; D011108:Polymers; C091381:poly(2-ethylacrylic acid)