Cold-induced gene expression profiles of Vibrio parahaemolyticus: a time-course analysis.
A whole-genome DNA microarray was constructed to dissect expression profiles of Vibrio parahaemolyticus in response to a sudden temperature downshift from 37 to 10 degrees C. The mRNA level of each gene at each of three time points (20, 40 and 60 min after temperature downshift) was compared with that just before cold treatment. Clustering analysis of time-course data revealed nine gene clusters with different time-dependent expression patterns. Downregulation of metabolism-related genes was obviously dominant over upregulation at all time points. The distinct negative regulation of metabolism-related genes would account for a generally reduced cellular protein pool resulting from the sudden temperature downshift. In contrast, cold shock had a 'neutral and balanced' regulatory action on nonmetabolic cellular pathways, which likely brought about the remodelling of cell envelope structures and transport/binding functions. We identified a 171-bp 5'-untranslated region in the cspA transcript. The cspA gene encoded cold shock protein A (CspA), and CspA was shown to be the major cold shock protein in V. parahaemolyticus. Evident regulatory motifs were conserved within the cspA promoter regions of Escherichia coli and V. parahaemolyticus. These two bacteria likely use the same mechanism to regulate the cold-inducible expression of cspA.
Yang
Lei
L
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Peking Union Medical College, Beijing, China.
Zhou
Dongsheng
D
Liu
Xiumei
X
Han
Haihong
H
Zhan
Lingjun
L
Guo
Zhaobiao
Z
Zhang
Lianfeng
L
Qin
Chuan
C
Wong
Hin-chung
HC
Yang
Ruifu
R
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003080:Cold Temperature; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins