Sensory denervation reduces visceral hypersensitivity in adult rats exposed to chronic unpredictable stress: evidences of neurogenic inflammation.
The purpose of this study is to provide evidence of neurogenic inflammation in chronic unpredictable stressed rats with the changes of visceral sensitivity, number of mast cells, and close proximity among mast cell-nerve-blood vessels. We found that (1) capsaicin denervation blocked stress-induced increase of visceral sensitivity, while doxantrazole presented a partial blocking; (2) capsaicin denervation blocked stress-induced enhancement of the proximity of mast cell-nerve fiber-blood vessels and blood vessel damage, while doxantrazole showed no effects on these; (3) doxantrazole blocked stress-induced increases of the MPO activity, the number and the degranulation of mast cells in the colon; (4) sensory denervation and doxantrazole had no effects on stress-induced behavioral inhibition. These results suggest that capsaicin-sensitive sensory fibers play a key role in stress-induced visceral hypersensitivity and the ultrastructural changes, mast cells play an important role in the generation of stress-induced colon inflammation.
Chen
Ji-Hong
JH
Division Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, People's Republic of China.
Wei
Shao-Zhong
SZ
Chen
Jian
J
Wang
Qing
Q
Liu
Hui-Lan
HL
Gao
Xiao-Hai
XH
Li
Guang-Can
GC
Yu
Wen-Zhen
WZ
Chen
Min
M
Luo
He-Sheng
HS
D000818:Animals; D001808:Blood Vessels / Q000294:innervation*; D002211:Capsaicin; D015550:Cell Degranulation; D003106:Colon / Q000201:enzymology / Q000276:immunology / Q000503:physiopathology* / Q000648:ultrastructure; D003714:Denervation; D005106:Exploratory Behavior; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors; D051381:Rats; D018689:Sensory System Agents; D013312:Stress, Physiological; D013315:Stress, Psychological / Q000201:enzymology / Q000276:immunology / Q000503:physiopathology*; D013892:Thioxanthenes; D044004:Xanthones
D010726:Phosphodiesterase Inhibitors; D018689:Sensory System Agents; D013892:Thioxanthenes; D044004:Xanthones; D009195:Peroxidase; D002211:Capsaicin; C010134:doxantrazole