title: Enzyme-linked immunosorbent assay method for detection of cytomegalovirus strain-specific antibody responses.
abstract: Reliable methods for the detection of cytomegalovirus (CMV) strain-specific serological responses are lacking. We describe a simple and reliable enzyme-linked immunosorbent assay method developed to detect antibodies against the polymorphic epitopes within the two envelope glycoproteins of CMV, glycoproteins H and B. This assay is useful for the detection of serologic responses to CMV strains and the identification of CMV reinfections.
authors.affiliation: Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, Alabama 35233, USA. zdnovak@peds.uab.edu
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Novak
authors.forename: Shannon A
authors.initials: SA
authors.lastname: Ross
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Patro
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Pati
authors.forename: Meera K
authors.initials: MK
authors.lastname: Reddy
authors.forename: Misty
authors.initials: M
authors.lastname: Purser
authors.forename: William J
authors.initials: WJ
authors.lastname: Britt
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Boppana
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C071440:glycoprotein H, Cytomegalovirus