CXCL5 gene polymorphism association with diabetes mellitus.
BACKGROUND
CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is a chemokine that has a role in the development of cardiovascular and other diseases. We have previously scanned the full length CXCL5 gene and reported the -156G>C (rs352046) polymorphism in the promoter region of this gene.


OBJECTIVE
The aim of this study was to examine whether there was an association between this polymorphism and type 2 diabetes mellitus or its microvascular complications in an Iranian population.


METHODS
A total of 230 patients with type 2 diabetes were recruited from Rafsanjan, in the south-east of Iran; 102 healthy control subjects were recruited from the same area. The region containing the CXCL5 -156G>C polymorphism was genotyped by PCR amplification and restriction fragment length polymorphism analysis, and allele frequency data were analyzed using STATA 8 software.


RESULTS
We observed that patients with type 2 diabetes had a higher frequency of carrying either the G/C or C/C genotype compared with healthy controls (C/G + C/C vs G/G; p = 0.004; odds ratio [OR] 2.17; 95% CI 1.27, 3.80). In addition, the frequency of allele C was significantly increased in patients with diabetes compared with controls (p = 0.01; OR 1.72; 95% CI 1.07, 2.86). No association was found between this polymorphism and diabetic microvascular complications.


CONCLUSIONS
Our findings suggest a role of CXCL5 in the pathogenesis of diabetes. The mechanism behind this role needs to be investigated further. Moreover, replications in other populations with larger sample sizes are required to confirm these findings.
Hasani Ranjbar
Shirin
S
Endocrinology and Metabolism Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
Amiri
Parvin
P
Zineh
Issam
I
Langaee
Taimour Y
TY
Namakchian
Mahsa
M
Heshmet
Ramin
R
Sajadi
Mohammadali
M
Mirzaee
Mohammadreza
M
Rezazadeh
Ebrahim
E
Balaei
Parisa
P
Tavakkoly Bazzaz
Javad
J
Gonzalez-Gay
Miguel A
MA
Larijani
Bagher
B
Amoli
Mahsa M
MM
D000328:Adult; D054365:Chemokine CXCL5 / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5