title: [Isolation, culture and purification of olfactory ensheathing cells from human fetal olfactory mucosa].
abstract: OBJECTIVE
  To explore the method for obtaining olfactory ensheathing cells from human fetal olfactory mucosa by cell culture for selective adhesion in the presence of neurotrophin-3 (NT3) and low-concentration serum.
  
  
  METHODS
  The olfactory ensheathing cells were cultured alternatively in DMEM/F12 culture medium containing 10% fetal bovine serum (FBS) and the medium containing NT3 and 2.5% FBS every 72 h. The cells were observed for morphological changes and identified using immunocytochemistry with P75NTR and GFAP, and the cell purity was estimated.
  
  
  RESULTS
  The olfactory ensheathing cells from human fetal olfactory mucosa were positive for P75(NTR) and GFAP, and in in vitro culture, the cells exhibited dipolar or tripolar appearance with long thin neurites. On the 9th day of cell culture, the purity of the olfactory ensheathing cells reached about 83%.
  
  
  CONCLUSIONS
  The olfactory ensheathing cells can be obtained by in vitro culture for selective adhesion in the presence of NT3 and low-concentration serum.
authors.affiliation: Department of Orthopedics, Oigdao Municipat Hospitat, Qingdao 266071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Xi-Jing
authors.initials: XJ
authors.lastname: He
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Zhu
authors.forename: Quan-Jin
authors.initials: QJ
authors.lastname: Zang
authors.forename: Pei
authors.initials: P
authors.lastname: Fan
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D006801:Humans; D020933:Neurotrophin 3 / Q000494:pharmacology; D009830:Olfactory Bulb / Q000166:cytology*; D009831:Olfactory Mucosa / Q000166:cytology*
chemical_list: D003470:Culture Media; D020933:Neurotrophin 3