[Caudal-related homeobox 2 gene expression and its effects on human gastric carcinoma cell line].
OBJECTIVE
To investigate the effect of human caudal-related homeobox 2 (Cdx2) gene expression on human gastric carcinoma cell line MGC-803.


METHODS
pCMV-Cdx2-HA eukaryotic expression plasmid was constructed by using DNA recombinant method. The MGC-803 cells were divided into 4 groups: non-transfected, transfected with pCMV-HA, transfected with pCMV-GAPDH-HA, transfected with pCMV-Cdx2-HA. Cdx2 gene and its protein expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot techniques respectively. The effects of Cdx2 overexpression on the growth of MGC-803 cells in vitro was assessed by measuring MTT, flow cytometry and transmission electron microscopy analysis.


RESULTS
In MGC-803 cells transfected with pCMV-Cdx2-HA, cell proliferation was significantly suppressed, the cells was ultrastructurally destroyed, cell cycle progression was blocked in G0/G1 and apoptosis rate was higher (P<0.05) in comparison with non-transfected cells or cells transfected with empty vector.


CONCLUSIONS
It indicated that Cdx2 gene can suppress the growth of gastric carcinoma and may save as a novel therapeutic target.
Li
Lei
L
Department of Surgery, the First Hospital Affiliated to Guangxi Medical University, Nanning 530021, China.
Xiao
Qiang
Q
Xie
Yu-bo
YB
Ma
Yu-lin
YL
Tang
Zhen-yong
ZY
Yin
Yong-shuo
YS
D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
D018398:Homeodomain Proteins; D012333:RNA, Messenger