[Development of bovine blood lead component analysis standard reference materials].
OBJECTIVE
To develop the first grade standard analytical reference material of bovine blood lead.


METHODS
According to standards and technology specification of primary standard reference material (JJG1006-1994), ISO 17511, and volume-effect relationship, a bovine blood lead model was developed by adding acetate lead in the feed in dose of 2-5 mg x kg(-1) x d(-1). Cow blood was collected when blood lead concentration went up to low, medium and high concentration range (90-100, 190-200, 280-300 microg/L). Blood sample was kept in tube and frozen after irradiation. The samples were tested for homogeneity and stability. ID-MS method was used to measure the lead concentration through comparison with two standard lead samples from the USA NIST SRM 955b.


RESULTS
Samples of the three lead concentrations showed uniformity by single factor analysis of variance (F = 0.61, 1.64, 0.28, respectively, P > 0.05) . After 14 months monitoring, the RSD was 0.85%, 1.05% and 0.49% (t = 0.787, 1.132, 0.854, respectively, P > 0.05). The characteristic and indefinite values were 102.4 +/- 5.5; 181.2 +/- 4.0; 304.5 +/- 3.9, respectively (unit: microg/L). The reproduction of the two USA NIST SRM 955b samples further showed our methods and procedures were correct. Our sample was stabile for four days at room temperature.


CONCLUSIONS
The standard reference material of bovine in our research had reached the national standard material requirements.
Li
Jian-ping
JP
Department of Medical Laboratory, Xi'an Central Hospital, Xi'an 710003, China.
Wu
Yi-qun
YQ
Zhao
Yong-gang
YG
Qi-Hong
Chi
Ai-jun
AJ
D000821:Animal Feed; D000818:Animals; D001774:Blood Chemical Analysis / Q000592:standards*; D002417:Cattle; D007854:Lead / Q000097:blood*; D008297:Male; D023421:Models, Animal; D012015:Reference Standards
D007854:Lead