title: [In vivo effect of nanophase Ti6Al4V on osseointegration: experiment with rabbits].
abstract: OBJECTIVE
  To evaluate the effect of nanophase Ti6Al4V substrates on the osseointegration in vivo.
  
  
  METHODS
  Novel nanophase Ti6Al4V substrates were prepared according to the severe plastic deformation principle. Eighteen New Zealand white rabbits were randomly divided into 3 equal groups with their trochanters of femur exposed and implanted with titanium substrate with common surface (Ti group), nanophase Ti6Al4V substrate (nano-Ti group), and hydroxyapatite-coated substrate (HA group) respectively. Four, 8, and 12 weeks later X-ray films were taken on 6 rabbits from each group, tetracycline and calcein were injected intramuscularly, and one day later the rabbits were sacrificed. The histological changes of the tissue surrounding the implant including the bone kinesics parameter were evaluated; the bone-implant interfaces were examined with scanning electron microscope (SEM) and transmission electron microscope (TEM) respectively.
  
  
  RESULTS
  Radiographic examinations showed that the bone recovery around the implant in the nano-Ti group was earlier compared to that in the Ti group. Histological examination suggested that the interface osseointegration rates 4, 8, and 12 weeks later of the nano-Ti groups were all significantly higher than those of the Ti group ( all P < 0.01). Strong tetracycline labeling and calcein labeling were observed around the implants in the nano-Ti group, indicating the active form action of new bone. The rates of bone mineralization and deposition 4, 8, and 12 weeks later of the nano-Ti group were higher than those of the Ti group. SEM and TEM examinations showed greater degradation of the surface and much more grains in cells in the HA group as compared to those in the nano-Ti group. The bone mineralization and osseointegration rates 4 weeks later of the HA group were significantly higher than those of the nano-Ti group (both P < 0.05), however, there were no significant differences in the bone mineralization and osseointegration rates 8 weeks later between these 2 groups. The bone mineralization and osseointegration rates 12 weeks later of the nano-Ti group were even higher than those of the HA group.
  
  
  CONCLUSIONS
  The novel nanophase Ti6Al4V substrates improves the bone-implant osseointegration without significant grains of degradation in vivo, suggesting that the novel substrates and nano technology should be further considered for the orthopedic implant applications.
authors.affiliation: Sixth People's Hospital of Shanghai Jiaotong University, Shanghai 200233, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Han
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Ji
authors.forename: Yao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Chang-Li
authors.initials: CL
authors.lastname: Zhao
authors.forename: Xiao-Nong
authors.initials: XN
authors.lastname: Zhang
mesh_terms: D000497:Alloys; D000818:Animals; D005269:Femur / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure; D020007:Implants, Experimental; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D016348:Osseointegration / Q000187:drug effects*; D011817:Rabbits; D011897:Random Allocation; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000497:Alloys; C031462:titanium alloy (TiAl6V4); D014025:Titanium