[Evaluation of microvessel density and vascular endothelial growth factor in colorectal carcinoma with 64-multidetector-row CT perfusion imaging].
OBJECTIVE
To evaluate the correlation of 64-multidetector-row CT (64MDCT) perfusion imaging with microvessel density(MVD) and vascular endothelial growth factor(VEGF) in colorectal carcinoma.


METHODS
64MDCT perfusion imaging was performed in 33 patients with pathologically verified colorectal carcinoma. Time-density curves (TDC) were created from the region of interest (ROI) drawn over the tumor, target artery and vein by 64MDCT with perfusion functional software. The individual perfusion maps generated were for blood flow (BF), blood volume (BV), mean transit time (MTT) and permeability-surface area product (PS). MVD and VEGF expression of surgical specimens were examined by immunohistochemical staining with anti-CD34, anti-VEGF monoclonal antibody. MVD and VEGF were compared among the different types of TDC in colorectal carcinoma. The correlation of CT perfusion parameters with MVD and VEGF was also examined.


RESULTS
TDC of colorectal carcinoma was divided into five types according to their shapes. MVD in the colorectal carcinoma was 22.61+/-9.01. VEGF staining was found in 25 of 29 tumors (86.2%). The score of VEGF expression was 4.15+/-1.09. No significant differences of MVD and VEGF expression among TDC types were found (F=2.59, 1.11, P>0.05). There were also no correlations of MVD and VEGF expression with any dynamic CT parameters (P>0.05).


CONCLUSIONS
64MDCT perfusion imaging, MVD and VEGF may reflect angiogenic activity, but no significant correlations are found among them.
Feng
Shi-ting
ST
Department of Radiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Sun
Can-hui
CH
Li
Zi-ping
ZP
Guo
Huan-yi
HY
Peng
Zhen-peng
ZP
Huang
Jian-wen
JW
Meng
Quan-fei
QF
Zheng
Ke-guo
KG
Xu
Da-sheng
DS
D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D008297:Male; D055806:Microvessels; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D036542:Tomography, Spiral Computed / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D055815:Young Adult
C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A