[Influence of gene transduction mediated by lentivirus vector on gene expression of human CD34+ cord blood cells].
OBJECTIVE
To investigate the influence of gene transduction mediated by lentivirus vector on human CD34+ cord blood cell (CBCs) gene expression.


METHODS
CD34+ cells were isolated and transduced with the third-generation self-inactivating ( SIN) lentiviral vector carrying green fluorescent protein (GFP). The total RNA from transduced cells was extracted and the differences of genotypes between the transduced and non-transduced CD34+ cells were determined with cDNA microarray analysis.


RESULTS
In 23000 genes two were upregulated and six downregulated. These changes were not confirmed by semi-quantitative RT-PCR method.


CONCLUSIONS
Lentiviral vector used in this study do not influence significantly on the gene expression of CD34+ CBCs, and the vector system may be a useful and safe one in clinical gene therapy.
Bai
Yuan-song
YS
Department of Hematology/Oncology, China-Japan Union Hospital of Jilin University, Changchun 130031, China.
Chen
Yu-bing
YB
Shi
Zhang-zhen
ZZ
Lu
Zhen-xia
ZX
D018952:Antigens, CD34; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D014161:Transduction, Genetic; D014162:Transfection
D018952:Antigens, CD34