title: Roles of the Aspergillus nidulans homologues of Tup1 and Ssn6 in chromatin structure and cell viability.
abstract: For three different carbon catabolite repressible promoters, alcA, alcR and the bidirectional promoter prnD-prnB, a deletion of rcoA, the Aspergillus nidulans homologue of TUP1, does not result in carbon catabolite derepression. Surprisingly, it results in disruption of the chromatin default structure of alcR and prnD-prnB promoters. In these promoters, and at variance with the wild type, repression occurs in the absence of nucleosome positioning. For alcR, repression occurs together with a nucleosome pattern identical to that found under conditions of full expression, and for prnD-prnB it occurs with a novel pattern that does not correspond to the pattern seen under conditions of repression in a wild-type strain. Deletion of the putative RcoA partner, SsnF, is lethal in A. nidulans.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis (CSIC-Universidad de Sevilla), Centro de Investigaciones Científicas Isla de la Cartuja, Seville, Spain. irene.garcia@ibvf.csic.es
authors.forename: Irene
authors.initials: I
authors.lastname: García
authors.forename: Martine
authors.initials: M
authors.lastname: Mathieu
authors.forename: Igor
authors.initials: I
authors.lastname: Nikolaev
authors.forename: Béatrice
authors.initials: B
authors.lastname: Felenbok
authors.forename: Claudio
authors.initials: C
authors.lastname: Scazzocchio
mesh_terms: D001233:Aspergillus nidulans / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D050296:Microbial Viability; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D002843:Chromatin; D005656:Fungal Proteins; D009707:Nucleosomes; D012097:Repressor Proteins; D002244:Carbon