title: MASDET-A fast and user-friendly multiplatform software for mass determination by dark-field electron microscopy.
abstract: Electron microscopy has been used to measure the mass of biological nanoparticles since the early 60s, and is the only way to obtain the mass of large structures or parameters such as the mass-per-length of filaments. The ability of this method to sort heterogeneous samples both in terms of mass and shape promises to make it a key tool for proteomics down to the single cell level. A new multiplatform software package, MASDET, that can be run under MATLAB or as a standalone program is described. Based on a user-friendly graphical interface MASDET streamlines mass evaluation and greatly increases the speed of required optimisation procedures. Importantly, the immediate application of Monte-Carlo simulations to describe multiple scattering is possible, allowing the mass analysis of thicker samples and the generation of mass thickness maps.
authors.affiliation: Institute of Medical Physics and Biophysics, Universitätsklinikum Münster, University of Münster, Germany. krzyzane@uni-muenster.de
authors.forename: Vladislav
authors.initials: V
authors.lastname: Krzyzánek
authors.forename: Shirley A
authors.initials: SA
authors.lastname: Müller
authors.forename: Andreas
authors.initials: A
authors.lastname: Engel
authors.forename: Rudolf
authors.initials: R
authors.lastname: Reichelt
mesh_terms: D003196:Computer Graphics; D003201:Computers; D003627:Data Interpretation, Statistical; D034301:Deinococcus / Q000378:metabolism; D004583:Electrons; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron / Q000379:methods*; D015233:Models, Statistical; D008970:Molecular Weight; D009010:Monte Carlo Method; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012984:Software; D014584:User-Computer Interface