title: Multilayer coatings on biomaterials for control of MG-63 osteoblast adhesion and growth.
abstract: Here, the layer-by-layer technique (LbL) was used to modify glass as model biomaterial with multilayers of chitosan and heparin to control the interaction with MG-63 osteoblast-like cells. Different pH values during multilayer formation were applied to control their physico-chemical properties. In the absence of adhesive proteins like plasma fibronectin (pFN) both plain layers were rather cytophobic. Hence, the preadsorption of pFN was used to enhance cell adhesion which was strongly dependent on pH. Comparing the adhesion promoting effects of pFN with an engineered repeat of the FN III fragment and collagen I which both lack a heparin binding domain it was found that multilayers could bind pFN specifically because only this protein was capable of promoting cell adhesion. Multilayer surfaces that inhibited MG-63 adhesion did also cause a decreased cell growth in the presence of serum, while an enhanced adhesion of cells was connected to an improved cell growth.
authors.affiliation: Biomedical Materials Group, Department of Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120, Halle (Saale), Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Kirchhof
authors.forename: Kamelia
authors.initials: K
authors.lastname: Hristova
authors.forename: Natalia
authors.initials: N
authors.lastname: Krasteva
authors.forename: George
authors.initials: G
authors.lastname: Altankov
authors.forename: Thomas
authors.initials: T
authors.lastname: Groth
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D005353:Fibronectins; D006801:Humans
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins