[Effect of coordination germanium compounds on biosynthesis and activity of proteases in Bacillus sp. and Yarrowia lipolytica].
The paper deals with the capacity of the coordination germanium compounds with citric acid in a complex with some aminoacids (aspartic acid, methionine, cysteine, threonine, phenylalanine, L-glutamic acid) as bioligands to affect synthesis and activity of the proteolytic enzyme complex of Bacillus sp. 27 and Yarrowia lipolytica 2061. Thus, biscitrategermanium acid H2[Ge(H4Citr)2] and its complex with aspartic acid (HAsp)2 [Ge(H4Citr)2] can be used with success for inducing biosynthesis ofcaseinolytic (182.1 and 197.4%) and elastase (182.6 and 383.3%) activities of Bacillus sp. 27, respectively. Biscitrategermanium acid can be used to induce biosynthesis of caseinolytic (182.1 and 197.4%) and elastase (182.6 and 383.3%) activities (respectively) of Bacillus sp. 27. Biscitrategermanium acid with methionine induced biosynthesis of caseinolytic (127.6%) and haemoglobinolytic (110.0%) activities of Y. lipolytica 2061. The tested coordinative compounds could not induce activity ofproteolytic complexes of both microorganisms. The capacity of complexes of citrategermanium acid with aspartic acid, methionine, cysteine, threonine, phenylalanine in concentration of 0.1% to inhibit activity of proteolytic complex of Y. lipolytica 2061 can be used in further studies of the catalytic centre of enzymes.
Shubchyns'ka
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Martsynko
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D000596:Amino Acids / Q000737:chemistry; D001407:Bacillus / Q000187:drug effects / Q000201:enzymology; D019343:Citric Acid / Q000737:chemistry; D005857:Germanium / Q000737:chemistry / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000378:metabolism; D025062:Yarrowia / Q000187:drug effects / Q000201:enzymology
D000596:Amino Acids; D005857:Germanium; D019343:Citric Acid; D010447:Peptide Hydrolases