title: Sensory denervation reduces visceral hypersensitivity in adult rats exposed to chronic unpredictable stress: evidences of neurogenic inflammation.
abstract: The purpose of this study is to provide evidence of neurogenic inflammation in chronic unpredictable stressed rats with the changes of visceral sensitivity, number of mast cells, and close proximity among mast cell-nerve-blood vessels. We found that (1) capsaicin denervation blocked stress-induced increase of visceral sensitivity, while doxantrazole presented a partial blocking; (2) capsaicin denervation blocked stress-induced enhancement of the proximity of mast cell-nerve fiber-blood vessels and blood vessel damage, while doxantrazole showed no effects on these; (3) doxantrazole blocked stress-induced increases of the MPO activity, the number and the degranulation of mast cells in the colon; (4) sensory denervation and doxantrazole had no effects on stress-induced behavioral inhibition. These results suggest that capsaicin-sensitive sensory fibers play a key role in stress-induced visceral hypersensitivity and the ultrastructural changes, mast cells play an important role in the generation of stress-induced colon inflammation.
authors.affiliation: Division Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, People's Republic of China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Shao-Zhong
authors.initials: SZ
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Liu
authors.forename: Xiao-Hai
authors.initials: XH
authors.lastname: Gao
authors.forename: Guang-Can
authors.initials: GC
authors.lastname: Li
authors.forename: Wen-Zhen
authors.initials: WZ
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: He-Sheng
authors.initials: HS
authors.lastname: Luo
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000294:innervation*; D002211:Capsaicin; D015550:Cell Degranulation; D003106:Colon / Q000201:enzymology / Q000276:immunology / Q000503:physiopathology* / Q000648:ultrastructure; D003714:Denervation; D005106:Exploratory Behavior; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors; D051381:Rats; D018689:Sensory System Agents; D013312:Stress, Physiological; D013315:Stress, Psychological / Q000201:enzymology / Q000276:immunology / Q000503:physiopathology*; D013892:Thioxanthenes; D044004:Xanthones
chemical_list: D010726:Phosphodiesterase Inhibitors; D018689:Sensory System Agents; D013892:Thioxanthenes; D044004:Xanthones; D009195:Peroxidase; D002211:Capsaicin; C010134:doxantrazole