title: Inflammation markers in healthy and periodontitis patients: a preliminary data screening.
abstract: Advances in diagnostic research are moving towards methods whereby the periodontal risk can be identified and quantified by objective measures using biomarkers. Patients with periodontitis may have elevated circulating levels of specific inflammatory markers that can be correlated to the severity of the disease. The purpose of this study was to evaluate whether differences in the serum levels of inflammatory biomarkers are differentially expressed in healthy and periodontitis patients. Twenty-five patients (8 healthy patients and 17 chronic periodontitis patients) were enrolled in the study. A 15 mL blood sample was used for identification of the inflammatory markers, with a human inflammatory flow cytometry multiplex assay. Among 24 assessed cytokines, only 3 (RANTES, MIG and Eotaxin) were statistically different between groups (p<0.05). In conclusion, some of the selected markers of inflammation are differentially expressed in healthy and periodontitis patients. Cytokine profile analysis may be further explored to distinguish the periodontitis patients from the ones free of disease and also to be used as a measure of risk. The present data, however, are limited and larger sample size studies are required to validate the findings of the specific biomarkers.
authors.affiliation: Department of Oral and Maxillofacial Surgery and Traumatology and Periodontics, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Adriana Corrêa
authors.initials: AC
authors.lastname: de Queiroz
authors.forename: Mario
authors.initials: M
authors.lastname: Taba
authors.forename: Patrícia Aquino
authors.initials: PA
authors.lastname: O'Connell
authors.forename: Priscila Brasil
authors.initials: PB
authors.lastname: da Nóbrega
authors.forename: Priscila Paganini
authors.initials: PP
authors.lastname: Costa
authors.forename: Viviane Keiko dos Santos
authors.initials: VK
authors.lastname: Kawata
authors.forename: Glauce Lunardelli
authors.initials: GL
authors.lastname: Trevisan
authors.forename: Arthur Belém
authors.initials: AB
authors.lastname: Novaes
authors.forename: Sergio Luís Scombatti
authors.initials: SL
authors.lastname: de Souza
authors.forename: Daniela Bazan
authors.initials: DB
authors.lastname: Palioto
authors.forename: Márcio Fernando de Moraes
authors.initials: MF
authors.lastname: Grisi
mesh_terms: D015415:Biomarkers / Q000097:blood; D018932:Chemokine CCL2 / Q000097:blood; D054405:Chemokine CCL3 / Q000097:blood; D054407:Chemokine CCL4 / Q000097:blood; D018946:Chemokine CCL5 / Q000097:blood; D054370:Chemokine CXCL9 / Q000097:blood; D019742:Chemokines, CC / Q000097:blood; D055113:Chronic Periodontitis / Q000097:blood*; D016207:Cytokines / Q000097:blood; D053222:Fas Ligand Protein / Q000097:blood; D016222:Fibroblast Growth Factor 2 / Q000097:blood; D005884:Gingival Hemorrhage / Q000097:blood; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D007371:Interferon-gamma / Q000097:blood; D016906:Interleukin-9 / Q000097:blood; D007378:Interleukins / Q000097:blood; D008233:Lymphotoxin-alpha / Q000097:blood; D017622:Periodontal Attachment Loss / Q000097:blood; D010514:Periodontal Pocket / Q000097:blood; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: D015415:Biomarkers; C111835:CCL2 protein, human; C515429:CCL3 protein, human; C483024:CCL5 protein, human; C080810:CXCL9 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054370:Chemokine CXCL9; D019742:Chemokines, CC; D016207:Cytokines; D053222:Fas Ligand Protein; D018836:Inflammation Mediators; D016906:Interleukin-9; D007378:Interleukins; D008233:Lymphotoxin-alpha; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor