A novel double-network hydrogel induces spontaneous articular cartilage regeneration in vivo in a large osteochondral defect.
We have developed a novel method to induce spontaneous hyaline cartilage regeneration in vivo for a large osteochondral defect by implanting a plug made from a double-network hydrogel composed of poly(2-acrylamido-2-methylpropanesulfonic acid) and poly(N,N'-dimethylacrylamide) at the bottom of the defect, leaving the cavity vacant. In cells regenerated in the treated defect, type-2 collagen, Aggrican, and SOX9 mRNAs were highly expressed and the regenerated matrix was rich in proteoglycan and type-2 collagen at 4 weeks. This fact gave a significant modification to the commonly established concept that hyaline cartilage tissue cannot regenerate in vivo. This study prompted an innovative strategy in the field of joint surgery to repair an osteochondral defect using an advanced, high-function hydrogel.
Yasuda
Kazunori
K
Department of Sports Medicine and Joint Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Kitamura
Nobuto
N
Gong
Jian Ping
JP
Arakaki
Kazunobu
K
Kwon
Hyuck Joon
HJ
Onodera
Shin
S
Chen
Yong Mei
YM
Kurokawa
Takayuki
T
Kanaya
Fuminori
F
Ohmiya
Yoshihiro
Y
Osada
Yoshihito
Y
D000178:Acrylamides / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002358:Cartilage, Articular / Q000293:injuries* / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000627:therapeutic use*; D007718:Knee Injuries / Q000601:surgery*; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use*; D011817:Rabbits; D012038:Regeneration / Q000502:physiology*; D055758:SOX9 Transcription Factor / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry / Q000627:therapeutic use*
D000178:Acrylamides; D001672:Biocompatible Materials; D011108:Polymers; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D013451:Sulfonic Acids; C429790:poly(N,N-dimethylacrylamide); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C077118:poly(2-acrylamido-2-methyl-1-propanesulfonic acid)