Self-assembled honokiol-loaded micelles based on poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) copolymer.
Self-assembled polymeric micelles are widely applied in drug delivery system (DDS). In this study, honokiol (HK) loaded micelles were prepared from biodegradable poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCEC) copolymers. Micelles were prepared by self-assembly of triblock copolymer PCEC in distilled water triggered by its amphiphilic character without any organic solvent. Drug loading and encapsulation efficiency were determined by adjusting the weight ratio of HK and PCEC. The particle size and zeta potential distribution of obtained micelles were determined using Malvern laser particle sizer, and spherical geometry were observed on atomic force microscope (AFM). Otherwise, the thermo-sensitivity of honokiol-loaded micelles was monitored. And the cytotoxicity results of drug loaded micelles showed that the encapsulated honokiol remained potent antitumor effect. Moreover, in vitro release profile demonstrated a significant difference between rapid release of free honokiol and much slower and sustained release of HK-loaded micelles. These results suggested that we have successfully prepared honokiol-loaded micelles in an improved method which is safer and more efficient. The prepared micelles might be potential carriers for honokiol delivery in cancer chemotherapy.
Wei
XiaWei
X
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Gong
ChangYang
C
Shi
Shuai
S
Fu
ShaoZhi
S
Men
Ke
K
Zeng
Shi
S
Zheng
XiuLing
X
Gou
MaLing
M
Chen
LiJuan
L
Qiu
LiYan
L
Qian
ZhiYong
Z
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage; D001713:Biphenyl Compounds / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D007834:Lasers; D017705:Lignans / Q000008:administration & dosage*; D008175:Lung Neoplasms / Q000188:drug therapy; D008823:Micelles; D018625:Microscopy, Atomic Force; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013696:Temperature; D000498:Allyl Compounds; D010636:Phenols
D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D017705:Lignans; D008823:Micelles; D011091:Polyesters; D011092:Polyethylene Glycols; C492324:poly(epsilon-caprolactone)-poly(oxyethylene)-poly(epsilon-caprolactone); C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols