title: Genetic diversity among Bolivian arenaviruses.
abstract: Machupo virus and Chapare virus are members of the Tacaribe serocomplex (virus family Arenaviridae) and etiological agents of hemorrhagic fever in humans in Bolivia. The nucleotide sequences of the complete Z genes, a large fragment of the RNA-dependent RNA polymerase genes, the complete glycoprotein precursor genes, and the complete nucleocapsid protein genes of 8 strains of Machupo virus were determined to increase our knowledge of the genetic diversity among the Bolivian arenaviruses. The results of analyses of the predicted amino acid sequences of the glycoproteins of the Machupo virus strains and Chapare virus strain 200001071 indicated that immune plasma from hemorrhagic fever cases caused by Machupo virus may prove beneficial in the treatment of Bolivian hemorrhagic fever but not hemorrhagic fever caused by Chapare virus.
authors.affiliation: Microbiology and Immunology Graduate Program, Graduate School of Biomedical Sciences, The University of Texas Medical Branch, Galveston, TX 77555-1019, USA. nbcajirm@utmb.edu
authors.forename: Maria N B
authors.initials: MN
authors.lastname: Cajimat
authors.forename: Mary Louise
authors.initials: ML
authors.lastname: Milazzo
authors.forename: Pierre E
authors.initials: PE
authors.lastname: Rollin
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Nichol
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Ksiazek
authors.forename: Charles F
authors.initials: CF
authors.lastname: Fulhorst
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001117:Arenaviridae Infections / Q000821:virology; D006477:Arenaviruses, New World / Q000145:classification / Q000235:genetics*; D001838:Bolivia; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics; D006478:Hemorrhagic Fever, American / Q000821:virology; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D006023:Glycoproteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D012324:RNA-Dependent RNA Polymerase