title: In vivo effects of intra-articular injection of gelatin hydrogen microspheres containing basic fibroblast growth factor on experimentally induced defects in third metacarpal bones of horses.
abstract: OBJECTIVE
  To evaluate the effect of intra-articular injection of gelatin hydrogel microspheres containing basic fibroblast growth factor (bFGF) on experimentally induced defects in third metacarpal bones (MC3s) of horses, in vivo.
  
  
  METHODS
  6 healthy adult Thoroughbreds.
  
  
  METHODS
  Horses were anesthetized, and a hole (diameter, 4.5 mm) was drilled into the medial condyle of both MC3s of each horse. One milliliter (100 microg) of a solution of gelatin hydrogel microspheres (2 mg) containing bFGF was injected into the joint capsule of the right metacarpophalangeal joint of each horse (bFGF joint). One milliliter of saline (0.9% NaCl) solution was injected into the left metacarpophalangeal joint (control joint). Radiography was performed 1 day and 4, 8, 12, and 16 weeks after surgery to evaluate bone defect refilling. Sixteen weeks after surgery, multidetector-row computed tomography (MDRCT) was performed to determine the degree of refilling at the bone defect site.
  
  
  RESULTS
  Radiography revealed healing of bone defects at 4 to 12 weeks after surgery in bFGF joints and at 8 to 16 weeks after surgery in control joints. In addition, MDRCT revealed a higher degree of healing in bFGF versus control joints. Mean+/-SD MDRCT score for bFGF joints (411.7+/-135.6 Hounsfield units) was significantly higher than that for control joints (240.8+/-133.1 Hounsfield units).
  
  
  CONCLUSIONS
  Treatment of horses with gelatin hydrogel microspheres that contained bFGF enhanced bone regeneration and healing of experimentally induced defects. This treatment strategy may be useful for treating horses with fractures.
authors.affiliation: Department of Veterinary Surgery, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sasaki
authors.forename: Takuto
authors.initials: T
authors.lastname: Minami
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Yamada
authors.forename: Haruo
authors.initials: H
authors.lastname: Yamada
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Inoue
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000293:injuries*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000627:therapeutic use*; D005552:Forelimb; D005780:Gelatin; D006734:Horse Diseases / Q000188:drug therapy*; D006736:Horses; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007270:Injections, Intra-Articular; D008297:Male
chemical_list: D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin