title: A synthetically simple, click-generated cyclam-based zinc(II) sensor.
abstract: A cyclam-based macrocyclic sensor has been prepared using synthetically simple "click" chemistry to link a fluorophore to the macrocyclic receptor. This sensor shows high selectivity for Zn(II) over a range of other metals, providing a significant enhancement of fluorescence intensity over a wide pH range. As such, this is the first cyclam-based sensor demonstrated to be selective for Zn(II) and is the first example of a triazole being used as a coordinating ligand on an azamacrocycle. The sensor can access biologically available zinc in mammalian cells, sensing the Zn(II) flux that exists during apoptotic cell death.
authors.affiliation: The Walter Besant Building, School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London, E1 4NS, United Kingdom.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Tamanini
authors.forename: Arna
authors.initials: A
authors.lastname: Katewa
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Sedger
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Todd
authors.forename: Michael
authors.initials: M
authors.lastname: Watkinson
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D004579:Electron Transport; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry*; D008027:Light; D051379:Mice; D014230:Triazoles / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D014230:Triazoles; C024560:cyclam; D002256:Carbonic Anhydrases; D015032:Zinc