title: Adenosine deaminase in patients with primary immunodeficiency syndromes: the analysis of serum ADA1 and ADA2 activities.
abstract: OBJECTIVE
  We aimed to investigate the activity of ADA and its isoenzymes in serum of patients with various primary immunodeficiency (PID) syndromes.
  
  
  METHODS
  Total ADA (tADA) and its isoenzymes were measured in 76 children with PID syndromes and 30 healthy controls using the Ellis method.
  
  
  RESULTS
  Our results indicated that tADA and ADA2 levels were higher in patients with Chronic Granulomatous Disease (CGD), Leukocyte Adhesion Deficiency (LAD), hyper IgM (HIM) and Wiskott-Aldrich Syndrome (WAS) than those of corresponding controls (P<0.01). There was a significant elevation of tADA and ADA1 activities in IgA deficiency patients as compared to healthy individuals (P<0.01).
  
  
  CONCLUSIONS
  Our results hypothesized that altered ADA activity may be associated with altered immunity. Therefore, serum ADA level could be used as an indicator along with other parameters in follow up of patients with CGD, LAD, IgA deficiency, HIM and WAS.
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran.
authors.forename: Pegah
authors.initials: P
authors.lastname: Poursharifi
authors.forename: Reza
authors.initials: R
authors.lastname: Saghiri
authors.forename: Mina
authors.initials: M
authors.lastname: Ebrahimi-Rad
authors.forename: Habibollah
authors.initials: H
authors.lastname: Nazem
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pourpak
authors.forename: Mostafa
authors.initials: M
authors.lastname: Moin
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Shams
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood* / Q000378:metabolism; D000293:Adolescent; D000704:Analysis of Variance; D002648:Child; D002675:Child, Preschool; D006105:Granulomatous Disease, Chronic / Q000201:enzymology; D006801:Humans; D053306:Hyper-IgM Immunodeficiency Syndrome / Q000201:enzymology; D017098:IgA Deficiency / Q000201:enzymology; D017099:IgG Deficiency / Q000201:enzymology; D007223:Infant; D007492:Iran; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000201:enzymology; D016511:Severe Combined Immunodeficiency / Q000201:enzymology* / Q000473:pathology
chemical_list: D007527:Isoenzymes; D000243:Adenosine Deaminase