title: Enhanced production of 1,2-propanediol by tpi1 deletion in Saccharomyces cerevisiae.
abstract: Saccharomyces cerevisiae was metabolically engineered to improve 1,2-propanediol production. Deletion of the tpi1 (triosephosphate isomerase) gene in S. cerevisiae increased the carbon flux to DHAP (dihydroxylacetone phosphate) in glycolysis, resulting in increased glycerol production. Then, the mgs and gldA genes, the products of which convert DHAP to 1,2-propanediol, were introduced to the tpi1-deficient strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of the mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both the mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in flask culture.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Korea.
authors.forename: Joon-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Eun-Sil
authors.initials: ES
authors.lastname: Choi
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
mesh_terms: D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D006019:Glycolysis; D019946:Propylene Glycol / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014305:Triose-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: D003470:Culture Media; D019946:Propylene Glycol; D011765:Pyruvaldehyde; D014305:Triose-Phosphate Isomerase; D005990:Glycerol