[Expression of programmed death 1 and its ligands in iris-ciliary body from mice with anterior chamber-associated immune deviation].
OBJECTIVE
To study the expression and possible implication of programmed death 1 (PD-1) and its ligands in the iris-ciliary body from mice with anterior chamber-associated immune deviation (ACAID).


METHODS
This was an experimental study. Twenty-four BALB/c mice were divided into ACAID group, negative controls, positive controls and phosphate-buffer saline controls. ACAID was evaluated by delayed-type hypersensitivity response. Expressions of PD-1 and its ligands (PD-L1, PD-L2) in the iris-ciliary body from ACAID mice were determined by real-time polymerase chain reaction and immunohistochemical studies.


RESULTS
Delayed-type hypersensitivity was not detected in ACAID group, indicating that ACAID was induced successfully. The expression of PD-1 mRNA in ACAID group was higher than that in all other groups (F = 248. 109, P < 0.05). The positive controls showed a high expression of PD-L1 mRNA (F = 179. 033, P < 0.05). PD-1 and PD-L1 positive cells with round or oval shapes were found in whole iris-ciliary body, especially in the iris base and pupil margin. Expression of PD-1 and PD-L1 proteins were similar to their expression at mRNA levels. Neither mRNA nor protein of PD-L2 was detected in the iris-ciliary body in all groups.


CONCLUSIONS
An increased expression of PD-1 in the iris-ciliary body of ACAID mice indicates that this is involved in the induction of ACAID.
Meng
Qian-li
QL
Zhongshan Ophthalmic Center, Uveitis Study Center, State Key Laboratory of Ophthalmology, Sun Yat-sen University, Guangzhou 510060, China.
Yang
Pei-zeng
PZ
Li
Bing
B
Zhou
Hong-yan
HY
Huang
Xiang-kun
XK
Zhu
Lian-xiang
LX
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002924:Ciliary Body / Q000378:metabolism*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000378:metabolism*; D007498:Iris / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D061026:Programmed Cell Death 1 Receptor
D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; D008024:Ligands; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor