Glucose sensor based on glucose oxidase-lipid LB film immobilized in Prussian Blue layer.
The structure and dynamic organization of a mixed Langmuir film of glucose oxidase (GOx) and lipid at the air-water interface were studied. The film was transferred onto the Prussian Blue (PB)-modified Pt electrode for biosensor preparation. The PB modified electrode showed well defined redox peaks in 0.1 M PBS electrolyte. The Langmuir film was characterized at the air-water interface by pi-A isotherms. The mixed monolayer was formed by spreading GOx on the LB trough covered with lipid. Time-pressure results show that at least 90 minutes are required to reach the equilibrium state of GOx-lipid film. The monolayer was transferred onto the PB-modified electrode when surface pressure was 40 mN/m. This sensor was characterized by a very low detection limit and a wide linear range. The optimal conditions for both fabricating and response of the sensor were investigated. The proposed biosensor showed a linear calibration range from 5 x 10(-6) to 6 x 10(-5) M. The detection limit was determined to be 1.5 x 10(-6) M.
Lee
Dong-Yun
DY
Department of Electrical Engineering and NTRC, Dong-A University, Busan 604-714, Korea.
Kafi
A K M
AK
Choi
Won-Suk
WS
Park
Sang-Hyun
SH
Kwon
Young-Soo
YS
D000388:Air; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D011312:Pressure; D015203:Reproducibility of Results; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry
D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D008055:Lipids; D014867:Water; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide