title: Development of multistage magnetic deposition microscopy.
abstract: Magnetic deposition microscropy (MDM) combines magnetic deposition and optical analysis of magnetically tagged cells into a single platform. Our multistage MDM uses enclosed microfabricated channels and a magnet assembly comprising four zones in series. The enclosed channels alleviate the problem plaguing previous versions of MDM: scouring of the cell deposition layer by the air-liquid interface as the channel is drained. The four-zone magnet assembly was designed to maximize capture efficiency, and experiments yielded total capture efficiencies of >99% of fluorescent- and magnetically-labeled Jurkat cells at reasonable throughputs (10(3) cells/min). A digital image processing protocol was developed to measure the average pixel intensities of the deposited cells in different zones, indicative of the marker expression. Preliminary findings indicate that the multistage MDM may be suitable for depositing cells and particles in successive zones according to their magnetic properties (e.g., magnetic susceptibilities or magnetophoretic mobilities). The overall goal is to allow the screening of multiple disease conditions in a single platform.
authors.affiliation: Department of Biomedical Engineering/ND-20, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.
authors.forename: Pulak
authors.initials: P
authors.lastname: Nath
authors.forename: Joseph
authors.initials: J
authors.lastname: Strelnik
authors.forename: Amit
authors.initials: A
authors.lastname: Vasanji
authors.forename: Lee R
authors.initials: LR
authors.lastname: Moore
authors.forename: P Stephen
authors.initials: PS
authors.lastname: Williams
authors.forename: Maciej
authors.initials: M
authors.lastname: Zborowski
authors.forename: Shuvo
authors.initials: S
authors.lastname: Roy
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Fleischman
mesh_terms: D001895:Boron / Q000737:chemistry; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007501:Iron / Q000737:chemistry; D019169:Jurkat Cells; D008280:Magnetics / Q000295:instrumentation / Q000379:methods*; D055616:Microtechnology / Q000295:instrumentation / Q000379:methods; D009354:Neodymium / Q000737:chemistry
chemical_list: D009354:Neodymium; D007501:Iron; D001895:Boron