title: Inhibition of neurosphere proliferation by IFNgamma but not IFNbeta is coupled to neuronal differentiation.
abstract: Interferons are produced following neural damage as part of the inflammatory response and may thus affect neural stem cell function. We compared the effects of interferon-gamma and interferon-beta on the proliferation and differentiation of adult murine neural progenitors. Both interferons inhibited neurosphere proliferation due to cell cycle arrest in G1 but only interferon-gamma induced neuronal differentiation. Both interferons induced differential phosphorylation of STAT proteins and a modest and late upregulation of the cell cycle regulator p27 but not several other likely cell cycle regulators. Thus in neural progenitor cells, anti-proliferative effects of interferons are not necessarily linked to differentiation.
authors.affiliation: Centre for Neuroscience, University of Melbourne VIC 3010, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Lum
authors.forename: E
authors.initials: E
authors.lastname: Croze
authors.forename: C
authors.initials: C
authors.lastname: Wagner
authors.forename: S
authors.initials: S
authors.lastname: McLenachan
authors.forename: B
authors.initials: B
authors.lastname: Mitrovic
authors.forename: A M
authors.initials: AM
authors.lastname: Turnley
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects*; D000704:Analysis of Variance; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D047468:Immunoprecipitation; D016899:Interferon-beta / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D020547:Lateral Ventricles / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects; D050791:STAT Transcription Factors / Q000378:metabolism; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D050791:STAT Transcription Factors; D014404:Tubulin; C462941:beta3 tubulin, mouse; D016899:Interferon-beta; D007371:Interferon-gamma