title: Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43.
abstract: N-Carbamoyl-L-amino-acid amidohydrolases (L-N-carbamoylases; EC 3.5.1.87) hydrolyze the carbon-nitrogen bond of the ureido group in N-carbamoyl-L-alpha-amino acids. These enzymes are commonly used in the production of optically pure natural and non-natural L-amino acids using the ;hydantoinase process'. Recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43 has been expressed, purified and crystallized by hanging-drop vapour diffusion. X-ray data were collected to a resolution of 2.75 A. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 103.2, b = 211.7, c = 43.1 A and two subunits in the asymmetric unit.
authors.affiliation: Departamento Química Física, Bioquímica y Química Inorgánica, Universidad de Almería, Almería, Spain. srodrig@ual.es
authors.forename: Sergio
authors.initials: S
authors.lastname: Martínez-Rodríguez
authors.forename: Abel
authors.initials: A
authors.lastname: García-Pino
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Las Heras-Vázquez
authors.forename: Josefa María
authors.initials: JM
authors.lastname: Clemente-Jiménez
authors.forename: Felipe
authors.initials: F
authors.lastname: Rodríguez-Vico
authors.forename: Remy
authors.initials: R
authors.lastname: Loris
authors.forename: Juan Ma
authors.initials: JM
authors.lastname: García-Ruiz
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Gavira
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000378:metabolism; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000581:Amidohydrolases