[Effects of piperazine ferulate on TGF-beta1-induced renal interstitial fibroblast activation].
OBJECTIVE
To investigate the effects of piperazine ferulate (PF) on TGF-beta1-induced renal interstitial fibroblast activation, and to explore the mechanism of PF in the prevention of renal tubulointerstitial fibrosis.


METHODS
In cultured normal rat renal kidney fibroblast (NRK-49F), the effect of PF on the TGF-beta1-induced cell vitality was observed by MTT. The protein expression levels of a-smooth muscle actin (alpha-SMA), connective tissue growth factor (CTGF) induced by TGF-beta1 were evaluated by immunocytochemistry. The levels of a-SMA, CTGF mRNA expression induced by TGF-beta1, were determined by quantitative real time fluroscent polymerase chain reaction. The levels of collagen type I (Col I) and fibronectin (FN) protein expression were examined by Enzyme Linked Immunosorbent Assay(ELISA).


RESULTS
TGF-beta1 may markedly increase the cell vitality, alpha-SMA and CTGF expression, FN and Col I protein expression (P < 0.05). Compared with TGF-beta1-induced group, PF can partly decrease the cell vitality, level of alpha-SMA and CTGF expression and extracellular matrix(ECM) synthesis induced by TGF-beta1 (P < 0.05).


CONCLUSIONS
PF may inhibit TGF-beta1-induced fibrosis in the renal fibroblast to a certain extent.
You
Guan-qiao
GQ
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Fu
Ping
P
Xie
Xi-sheng
XS
Liu
Fei
F
Li
Jing
J
D000199:Actins / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D003373:Coumaric Acids / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005355:Fibrosis / Q000517:prevention & control; D007668:Kidney / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D000077489:Piperazine; D010879:Piperazines / Q000494:pharmacology*; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
D000199:Actins; D055513:Connective Tissue Growth Factor; D003373:Coumaric Acids; D000077489:Piperazine; D010879:Piperazines; D053773:Transforming Growth Factor beta1; C498862:Acta2 protein, rat; C004999:ferulic acid