GAPDH as housekeeping gene for human skin fibroblast senescent model.
Validation of housekeeping gene is important for accurate quantitation of RNA in real time RT-PCR technique. The purpose of this study was to determine the validity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a housekeeping gene for quantitative real time RT-PCR assessment in human skin fibroblast senescent model. The cells were divided into different treatment groups; young (passage 4), senescent (passage 30), treatment with H2O2 and treatment with A-tocotrienol prior to H2O2 treatment. Our results showed that the expression level of GAPDH was constant with different treatment groups. Therefore, we concluded that GAPDH was suitable to be used as housekeeping gene in human skin fibroblast senescent model.
Zainuddin
A
A
Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Makpol
S
S
Chua
K H
KH
Abdul Rahim
N
N
Yusof
Y A Mohd
YA
Ngah
W Z Wan
WZ
D000975:Antioxidants / Q000378:metabolism*; D016922:Cellular Senescence / Q000502:physiology*; D005347:Fibroblasts / Q000201:enzymology / Q000502:physiology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D008962:Models, Theoretical; D012867:Skin / Q000201:enzymology*; D012879:Skin Physiological Phenomena; D024508:Tocotrienols / Q000378:metabolism*
D000975:Antioxidants; D024508:Tocotrienols; D006861:Hydrogen Peroxide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases