title: [Association between tumor necrosis factor alpha gene polymorphism and colorectal cancer].
abstract: OBJECTIVE
  To elucidate the association of TNF-alpha-308G/A gene functional polymorphism with the development and progression of colorectal cancer.
  
  
  METHODS
  PCR-RFLP was employed to detect the TNF-alpha-308 G/A genotypes in 157 colorectal cancer patients and 117 healthy controls.
  
  
  RESULTS
  The frequency of TNF-alpha-308 genotype and allele were not significantly different between colorectal cancer patients and healthy controls (genotype chi(2)=1.054, P=0.591, allele chi(2)=0.404, P=0.525). The frequency of A/A genotype and A allele in III+IV stage (62 patients in total) were higher than those in I+II stages (85 patients in total) (A allele: 22.6% vs 12.9%, A/A genotype: 8.1% vs 1.2%), and the differences were significant (genotype P=0.048, OR=7.368, 95% CI=0.839-64.743, allele chi(2)=4.720, P=0.03, OR=1.962, 95% CI=1.061-3.628). The frequency of TNF-alpha-308 genotype were not significantly different among different colorectal cancer grades (chi(2)=3.009,P=0.591).
  
  
  CONCLUSIONS
  TNF-alpha-308G/A gene polymorphism is not associated with the development of colorectal cancer, but TNF-alpha-308 A/A genotype and A allele are related to the progression of colorectal cancer.
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen 361004, China. wuguoyangmail@yahoo.com.cn
authors.forename: Guo-yang
authors.initials: GY
authors.lastname: Wu
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Wang
authors.forename: Michael
authors.initials: M
authors.lastname: Keese
authors.forename: Till
authors.initials: T
authors.lastname: Hasenberg
authors.forename: Jörg W
authors.initials: JW
authors.lastname: Sturm
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha