title: Plasma and placental nitric oxide levels in women with and without pre-eclampsia living at different altitudes.
abstract: OBJECTIVE
  To investigate the nitric oxide (NO) levels in the plasma and the placentas of pregnant women with pre-eclampsia and women without pre-eclampsia, and to determine the effect of high or low altitude of residence.
  
  
  METHODS
  NO was determined by chemoluminescence and group comparisons were performed.
  
  
  RESULTS
  Women with pre-eclampsia (n=63) had higher plasma NO levels (38.6+/-17.44 vs 30.6+/-12.44 micromol/L, P=0.004) and higher placental NO levels (38.5+/-17.0 vs 24.3+/-7.16 ng/mg protein, P<0.05) compared with women without pre-eclampsia. A similar trend was found when comparisons were made according to altitude of residence. NO levels were significantly higher in the plasma of pre-eclamptic women living at sea level (41.11+/-18.78 vs 28.96+/-9.57 micromol/L, P=0.003), and in the placentas of women living at high altitude (39.51+/-16.98 vs 21.91+/-6.64 ng/mg protein, P<0.0001).
  
  
  CONCLUSIONS
  Women with pre-eclampsia had higher plasma and placental NO levels and the differences were associated with altitude of residence.
authors.affiliation: Biomedical Center, Central University of Ecuador, Quito, Ecuador. e_teran_uce@yahoo.com
authors.forename: Enrique
authors.initials: E
authors.lastname: Teran
authors.forename: Peter
authors.initials: P
authors.lastname: Chedraui
authors.forename: Sandra
authors.initials: S
authors.lastname: Vivero
authors.forename: Francisco
authors.initials: F
authors.lastname: Villena
authors.forename: Fabian
authors.initials: F
authors.lastname: Duchicela
authors.forename: Luis
authors.initials: L
authors.lastname: Nacevilla
mesh_terms: D000293:Adolescent; D000328:Adult; D000531:Altitude; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000097:blood*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D009569:Nitric Oxide