title: A birth from the transfer of a single vitrified-warmed blastocyst using intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient.
abstract: OBJECTIVE
  To present the effectiveness of diagnostic heterologous intracytoplasmic sperm injection (ICSI), mouse oocyte activation test (MOAT), and ICSI combined with assisted oocyte activation (AOA) in a globozoospermic patient.
  
  
  METHODS
  A case report.
  
  
  METHODS
  A private IVF center, Japan.
  
  
  METHODS
  A patient with globozoospermia.
  
  
  METHODS
  MOAT in a mouse and ICSI combined with AOA in a human.
  
  
  METHODS
  Ultrastructure, MOAT, fertilization, and pregnancy.
  
  
  RESULTS
  The transmission electron micrographs showed 100% round-headed spermatozoa lacking an acrosome. MOAT showed that the fertilization rate was 68.4% (13/19) when AOA was used but 0% (0/19) when AOA was not used. After the diagnosis of globozoospermia and sperm-related activation deficiency, 17 human mature oocytes were activated with calcium ionophore after ICSI was performed. The fertilization rate was 88.2% (15/17), and 11 blastocysts were cryopreserved using the vitrification method to prevent severe ovarian hyperstimulation syndrome. A single vitrified-warmed blastocyst was transferred. A gestational sac with fetal heart movements was recognized, and a healthy boy weighing 3180 g was born at 40 weeks of gestation by cesarean section without any congenital abnormality.
  
  
  CONCLUSIONS
  MOAT allows discrimination between sperm- and oocyte-related fertilization failures and shows the effectiveness of AOA.
authors.affiliation: Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan. info@ivf-kyono.or.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Kyono
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakajo
authors.forename: Chikako
authors.initials: C
authors.lastname: Nishinaka
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Hattori
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kyoya
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Araki
mesh_terms: D000328:Adult; D000818:Animals; D001681:Biological Assay; D000001:Calcimycin / Q000627:therapeutic use*; D002118:Calcium / Q000378:metabolism*; D002585:Cesarean Section; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000628:therapy*; D007476:Ionophores / Q000627:therapeutic use*; D050498:Live Birth; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D054315:Oocyte Retrieval; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D010062:Ovulation Induction; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000648:ultrastructure
chemical_list: D007476:Ionophores; D000001:Calcimycin; D002118:Calcium