title: OcuDrain-E-A noninvasive technique for reduction of intraocular pressure.
abstract: OcuDrain-E is a noninvasive technique in which electrical pulses are applied across the cornea to enhance the rate of transcorneal water evaporation (TCWE). In vitro studies were carried out with rabbit cornea mounted on a Franz diffusion cell. Application of 30 pulses each of 1millisecond (ms) duration at >or=40V/cm(2) decreased the corneal resistivity approximately 80% indicating permeabilization of the cornea. The corneal resistivity was almost completely recovered within 6h when the pulse voltage was <40V/cm(2). The average TCWE at 40V/cm(2) was significantly (approximately 39-fold) higher than the control (t-test, p<0.0001). Application of electrical pulses (40V-30 pulses-1ms-1Hz) across the cornea resulted in significant decrease in the intraocular pressure (IOP) in rabbits. The electrical protocol was well tolerated by the rabbits. Microscopic studies revealed that the applied electrical protocol did not cause any edema or detachment of the epidermal layers. The results of current investigation suggest that OcuDrain-E could be developed as a potential technique for the treatment of glaucoma in patients who respond poorly to drugs.
authors.affiliation: Department of Pharmaceutics, The University of Mississippi, University, MS 38677, United States. murthy@olemiss.edu
authors.forename: S Narasimha
authors.initials: SN
authors.lastname: Murthy
authors.forename: Srinivasa M
authors.initials: SM
authors.lastname: Sammeta
authors.forename: Siva Ram K
authors.initials: SR
authors.lastname: Vaka
mesh_terms: D000818:Animals; D003315:Cornea / Q000378:metabolism*; D015194:Diffusion Chambers, Culture; D004560:Electricity / Q000009:adverse effects; D005901:Glaucoma / Q000628:therapy; D006801:Humans; D066298:In Vitro Techniques; D007429:Intraocular Pressure; D010539:Permeability; D011817:Rabbits; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water