Granulocyte macrophage--colony-stimulating factor-dependent proliferation is impaired in macrophages from senescence-accelerated mice.
A senescence-accelerated (SAMP8) mouse model was used to determine the effect of aging on the immune system. We produced in vitro bone marrow-derived macrophages from SAMP8 mice and compared them against senescence-resistant, long-lived mice (SAMR1). Although macrophages from both strains of mice proliferated in a similar manner in response to monocyte-colony-stimulating factor (M-CSF), SAMP8 macrophages showed an impaired response to granulocyte macrophage-colony-stimulating factor (GM-CSF). Similar levels of external regulated kinases (ERK)1/2 and signaling transducer and activator of transcription 5 (STAT5) phosphorylation were observed in macrophages from both strains of mice. The lack of proliferation was not caused by the induction of apoptosis. Differentiation of bone marrow cells into dendritic cells was similar in both strains of mice, as was the induction of major histocompatibility complex (MHC) class II molecules by interferon-gamma (IFN-gamma). Finally, we determined the density of Langerhans cells in vivo in the skin of the two mouse strains, but no differences were found.
Espía
Marta
M
Macroophage Biology Group, Institute for Research in Biomedicine, Barcelona, Barcelona Science Park, C/ Josep Samitier 1-5, E-08028 Barcelona, Spain.
Sebastián
Carlos
C
Mulero
Miquel
M
Giralt
Montserrat
M
Mallol
Jordi
J
Celada
Antonio
A
Lloberas
Jorge
J
D000375:Aging / Q000276:immunology*; D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007801:Langerhans Cells / Q000166:cytology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D010766:Phosphorylation; D011994:Recombinant Proteins; D050799:STAT5 Transcription Factor / Q000378:metabolism; D012867:Skin / Q000166:cytology
D011994:Recombinant Proteins; D050799:STAT5 Transcription Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor