[Study of DNA extraction methods for testing for genetically modified organisms in soyproducts].
In order to evaluate three different methods for DNA extraction (CTAB, DNeasy Plant Mini Kit and Wizard DNA Clean-up Resin system), the yields of DNA extracted from soyproducts and the copy numbers of lectin genes amplified by quantitative PCR were compared. Fermented foods, such as miso and nattou, gave poor yields of DNA and low copy numbers with any method. However atsu-age and kinugoshi-tofu gave high-quality results with all methods. Kinako gave a high yield of DNA, but poor amplification. Boiled soybeans and soymilk showed in poor amplification. It is important to choose the appropriate DNA extraction method for each product.
Moriuchi
Rie
R
Tokyo Metropolitan Institute of Public Health, 3241, Hyakunin-cho, Shinjuku-ku, Tokyo 1690073, Japan.
Monma
Kimio
K
Kamata
Kunihiro
K
Ibe
Akihiro
A
D018744:DNA, Plant / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D045730:Soy Foods / Q000032:analysis*
D018744:DNA, Plant