title: Structural basis for putrescine activation of human S-adenosylmethionine decarboxylase.
abstract: Putrescine (1,4-diaminobutane) activates the autoprocessing and decarboxylation reactions of human S-adenosylmethionine decarboxylase (AdoMetDC), a critical enzyme in the polyamine biosynthetic pathway. In human AdoMetDC, putrescine binds in a buried pocket containing acidic residues Asp174, Glu178, and Glu256. The pocket is away from the active site but near the dimer interface; however, a series of hydrophilic residues connect the putrescine binding site and the active site. Mutation of these acidic residues modulates the effects of putrescine. D174N, E178Q, and E256Q mutants were expressed and dialyzed to remove putrescine and studied biochemically using X-ray crystallography, UV-CD spectroscopy, analytical ultracentrifugation, and ITC binding studies. The results show that the binding of putrescine to the wild type dimeric protein is cooperative. The D174N mutant does not bind putrescine, and the E178Q and E256Q mutants bind putrescine weakly with no cooperativity. The crystal structure of the mutants with and without putrescine and their complexes with S-adenosylmethionine methyl ester were obtained. Binding of putrescine results in a reorganization of four aromatic residues (Phe285, Phe315, Tyr318, and Phe320) and a conformational change in the loop 312-320. The loop shields putrescine from the external solvent, enhancing its electrostatic and hydrogen bonding effects. The E256Q mutant with putrescine added shows an alternate conformation of His243, Glu11, Lys80, and Ser229, the residues that link the active site and the putrescine binding site, suggesting that putrescine activates the enzyme through electrostatic effects and acts as a switch to correctly orient key catalytic residues.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Shridhar
authors.initials: S
authors.lastname: Bale
authors.forename: Maria M
authors.initials: MM
authors.lastname: Lopez
authors.forename: George I
authors.initials: GI
authors.lastname: Makhatadze
authors.forename: Qingming
authors.initials: Q
authors.lastname: Fang
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Pegg
authors.forename: Steven E
authors.initials: SE
authors.lastname: Ealick
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D019281:Dimerization; D004789:Enzyme Activation / Q000502:physiology; D006801:Humans; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D011700:Putrescine / Q000737:chemistry* / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D012437:Adenosylmethionine Decarboxylase; D011700:Putrescine