Chemical strategies for generating protein biochips.
Protein biochips are at the heart of many medical and bioanalytical applications. Increasing interest has been focused on surface activation and subsequent functionalization strategies for immobilizing these biomolecules. Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function. Strategies for creating protein patterns (as opposed to protein arrays) are also outlined. An outlook on promising and challenging future directions for protein biochip research and applications is also offered.
Jonkheijm
Pascal
P
Department of Chemical Biology, Max Planck Institute of Molecular Physiology and Faculty of Chemistry, Chemical Biology, Technical University of Dortmund, Otto Hahn Strasse 11, 44227 Dortmund, Germany.
Weinrich
Dirk
D
Schröder
Hendrik
H
Niemeyer
Christof M
CM
Waldmann
Herbert
H
D055767:Immobilized Proteins / Q000737:chemistry*; D040081:Protein Array Analysis; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
D055767:Immobilized Proteins; D012825:Silicon