[Study of polymerizing hemoglobin on cation exchange chromatography].
Poly-hemoglobin is the active component of hemoglobin-based blood substitutes. The excess reactivity of glutaraldehyde with hemoglobin in solution leads to poly-hemoglobin of a wide molecular weight distribution and a high average molecular weight. A new polymerization method has been tested to decrease the molecular weight distribution and the average molecular weight. The poly-hemoglobin with lower degree of modification (polymerization) was found enriched on the cation exchange columns and further polymerized with glutaraldehyde. The poly-hemoglobin of narrower molecular weight distribution has been prepared in this way.
Wang
Xiang
X
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300071, China. tpuwx@sohu.com
Huang
Lei
L
Yang
Chenming
C
Wang
Jinfeng
J
D001802:Blood Substitutes / Q000138:chemical synthesis* / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D001802:Blood Substitutes; D006454:Hemoglobins; C030939:polyhemoglobin-pyridoxal-5-phosphate; D011732:Pyridoxal Phosphate