title: In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry.
abstract: In vitro replicas of bone marrow can potentially provide a continuous source of blood cells for transplantation and serve as a laboratory model to examine human immune system dysfunctions and drug toxicology. Here we report the development of an in vitro artificial bone marrow based on a 3D scaffold with inverted colloidal crystal (ICC) geometry mimicking the structural topology of actual bone marrow matrix. To facilitate adhesion of cells, scaffolds were coated with a layer of transparent nanocomposite. After seeding with hematopoietic stem cells (HSCs), ICC scaffolds were capable of supporting expansion of CD34+ HSCs with B-lymphocyte differentiation. Three-dimensional organization was shown to be critical for production of B cells and antigen-specific antibodies. Functionality of bone marrow constructs was confirmed by implantation of matrices containing human CD34+ cells onto the backs of severe combined immunodeficiency (SCID) mice with subsequent generation of human immune cells.
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA. jnichols@utmb.edu
authors.forename: Joan E
authors.initials: JE
authors.lastname: Nichols
authors.forename: Joaquin
authors.initials: J
authors.lastname: Cortiella
authors.forename: Jungwoo
authors.initials: J
authors.lastname: Lee
authors.forename: Jean A
authors.initials: JA
authors.lastname: Niles
authors.forename: Meghan
authors.initials: M
authors.lastname: Cuddihy
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Wang
authors.forename: Joseph
authors.initials: J
authors.lastname: Bielitzki
authors.forename: Andrea
authors.initials: A
authors.lastname: Cantu
authors.forename: Ron
authors.initials: R
authors.lastname: Mlcak
authors.forename: Esther
authors.initials: E
authors.lastname: Valdivia
authors.forename: Ryan
authors.initials: R
authors.lastname: Yancy
authors.forename: Matthew L
authors.initials: ML
authors.lastname: McClure
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Kotov
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D040761:Biomimetic Materials; D001853:Bone Marrow / Q000033:anatomy & histology*; D002454:Cell Differentiation; D002460:Cell Line; D003102:Colloids; D003460:Crystallization; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D003102:Colloids