title: Deletion of nonstructural proteins NS1 and NS2 from pneumonia virus of mice attenuates viral replication and reduces pulmonary cytokine expression and disease.
abstract: Pneumonia virus of mice (PVM) strain 15 causes fatal pneumonia in mice and provides a convenient model for human respiratory syncytial virus pathogenesis and immunobiology. We prepared PVM mutants lacking the genes for nonstructural proteins NS1 and/or NS2. In Vero cells, which lack type I interferon (IFN), deletion of these proteins had no effect on the efficiency of virus growth. In IFN-competent mouse embryo fibroblasts, wild-type (wt) PVM and the DeltaNS1 virus grew efficiently and strongly inhibited the IFN response, whereas virus lacking NS2 was highly attenuated and induced high levels of IFN and IFN-inducible genes. In BALB/c mice, intranasal infection with wt PVM caused overt disease that began on day 6 and was lethal by day 9 postinoculation. In comparison, DeltaNS1 induced transient, reduced disease, and DeltaNS2 and DeltaNS12 caused no disease. Thus, NS1 and NS2 are virulence factors, with NS2 being a major antagonist of the type I IFN system. The pulmonary titers of wt PVM and DeltaNS1 were high on day 3 and increased further by day 6; in addition, expression of IFN and representative proinflammatory cytokines/chemokines and T lymphocyte-related cytokines was undetectable on day 3 but increased dramatically by day 6 coincident with the onset of disease. The titers of DeltaNS2 and DeltaNS12 were somewhat lower on day 3 and decreased further by day 6; in addition, these viruses induced a more circumscribed set of cytokines/chemokines (IFN, interleukin-6 [IL-6], and CXCL10) that were detected on day 3 and had largely subsided by day 6. Lung immunohistology revealed abundant PVM-positive pneumocytes and bronchial and bronchiolar epithelial cells in wt PVM- and DeltaNS1-infected mice on day 6 compared to few PVM-positive foci with DeltaNS2 and DeltaNS12. These results indicate that severe PVM disease is associated with high, poorly controlled virus replication driving the expression of high levels of pulmonary IFN and a broad array of cytokines/chemokines. In contrast, in the absence of NS2, there was an early, transient innate response involving moderate levels of IFN, IL-6, and CXCL10 that restricted virus replication and prevented disease.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA. ubuchholz@niaid.nih.gov
authors.forename: Ursula J
authors.initials: UJ
authors.lastname: Buchholz
authors.forename: Jerrold M
authors.initials: JM
authors.lastname: Ward
authors.forename: Elaine W
authors.initials: EW
authors.lastname: Lamirande
authors.forename: Britta
authors.initials: B
authors.lastname: Heinze
authors.forename: Christine D
authors.initials: CD
authors.lastname: Krempl
authors.forename: Peter L
authors.initials: PL
authors.lastname: Collins
mesh_terms: D000818:Animals; D001835:Body Weight; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D017353:Gene Deletion; D008168:Lung / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D029122:Murine pneumonia virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*; D012720:Severity of Illness Index; D016019:Survival Analysis; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
chemical_list: D016207:Cytokines; D017361:Viral Nonstructural Proteins; D037521:Virulence Factors