Reversible switching of pRNA activity on the DNA packaging motor of bacteriophage phi29.
This paper reports a reversible switching of the biological activity of an RNA molecule, packaging RNA (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29. The switching mechanism contains two components: (1) inhibition of pRNA by a short antisense DNA (asDNA) that can bind to the 3' end of the pRNA and inactivate the packaging motor; and (2) reactivation of pRNA by isothermal removal of asDNA from pRNA through a strand displacement strategy. The switching process can be repeated for multiple cycles and has been demonstrated by gel electrophoresis and a virion assembly assay.
Ko
Seung Hyeon
SH
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, USA.
Chen
Yi
Y
Shu
Dan
D
Guo
Peixuan
P
Mao
Chengde
C
D017103:Bacillus Phages / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D042003:DNA Packaging; D016373:DNA, Antisense / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins; D014779:Virus Replication
D016373:DNA, Antisense; D004279:DNA, Viral; D020409:Molecular Motor Proteins; D012367:RNA, Viral; D014764:Viral Proteins