title: Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection.
abstract: Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more resistant against killing by neutrophils than the wild type and any other of the more than 3000 tested mutants. Inactivation of pilY1 led to the loss of twitching motility in twitching-proficient wild-type PA14 and PAO1 strains, predisposed to autolysis and impaired the secretion of quinolones and pyocyanin, but on the other hand promoted growth in stationary phase and bacterial survival in murine airway infection models. The PilY1 population consisted of a major full-length and a minor shorter PilY1* isoform. PilY1* was detectable in small extracellular quinolone-positive aggregates, but not in the pilus. P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population.
authors.affiliation: Klinische Forschergruppe, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse, Hannover, Germany.
authors.forename: Yu-Sing Tammy
authors.initials: YS
authors.lastname: Bohn
authors.forename: Gudrun
authors.initials: G
authors.lastname: Brandes
authors.forename: Elza
authors.initials: E
authors.lastname: Rakhimova
authors.forename: Sonja
authors.initials: S
authors.lastname: Horatzek
authors.forename: Prabhakar
authors.initials: P
authors.lastname: Salunkhe
authors.forename: Antje
authors.initials: A
authors.lastname: Munder
authors.forename: Andrea
authors.initials: A
authors.lastname: van Barneveld
authors.forename: Doris
authors.initials: D
authors.lastname: Jordan
authors.forename: Florian
authors.initials: F
authors.lastname: Bredenbruch
authors.forename: Susanne
authors.initials: S
authors.lastname: Häussler
authors.forename: Kathrin
authors.initials: K
authors.lastname: Riedel
authors.forename: Leo
authors.initials: L
authors.lastname: Eberl
authors.forename: Peter Østrup
authors.initials: PØ
authors.lastname: Jensen
authors.forename: Thomas
authors.initials: T
authors.lastname: Bjarnsholt
authors.forename: Claus
authors.initials: C
authors.lastname: Moser
authors.forename: Niels
authors.initials: N
authors.lastname: Hoiby
authors.forename: Burkhard
authors.initials: B
authors.lastname: Tümmler
authors.forename: Lutz
authors.initials: L
authors.lastname: Wiehlmann
mesh_terms: D000818:Animals; D001692:Biological Transport; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D051379:Mice; D008809:Mice, Inbred C3H; D016296:Mutagenesis; D009504:Neutrophils / Q000382:microbiology; D010619:Phenazines / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010619:Phenazines; C103855:PilY1 protein, Pseudomonas aeruginosa; D020033:Protein Isoforms; D011804:Quinolines; D036781:Fimbriae Proteins