Candida kanchanaburiensis sp. nov., a new ascomycetous yeast species related to Pichia nakazawae isolated in Thailand.
Three yeast strains, ST-633, ST-634 and ST-635, isolated from the fruit body of a mushroom, Coprinus sp., and rotted fruit of guava collected in the western region of Thailand, were found to represent a hitherto undescribed species. This yeast is related to Pichia nakazawae var. akitaensis, P. nakazawae var. nakazawae and Pichia philogaea in the D1/D2 domain of 26S rDNA but 12 (2.3%), 13 (2.5%) and 15 (2.8%) nucleotides are different from these taxa, respectively, suggesting the distinctness of the Thai strains at species level. Since ascospore formation was not detected, it is described as a new species of Candida, Candida kanchanaburiensis. This species is distinguished from P. nakazawae by the ability to assimilate 2-ketogluconic acid and L-lysine, and inability to assimilate soluble starch.
Nakase
Takashi
T
NITE Biological Resource Center, Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, Japan nakase-takahashi@nite-go.jp
Jindamorakot
Sasitorn
S
Ninomiya
Shinya
S
Imanishi
Yumi
Y
Kawasaki
Hiroko
H
Potacharoen
Wanchern
W
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003302:Coprinus; D004271:DNA, Fungal / Q000032:analysis; D005942:Gluconates / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010641:Phenotype; D010802:Phylogeny; D010843:Pichia / Q000145:classification* / Q000235:genetics; D031647:Psidium / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013785:Thailand
D004271:DNA, Fungal; D005942:Gluconates; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; C008229:2-ketogluconate; D008239:Lysine