Trim36/Haprin plays a critical role in the arrangement of somites during Xenopus embryogenesis.
The TRIM family members contain a tripartite motif (TRIM), which includes RING, B-box, and coiled-coil domains, or collectively RBCC. They have been implicated in a variety of biological processes, such as the regulation of differentiation and development, and oncogenesis. In this study, we discovered a novel function of the TRIM family in early development. We report the expression of Trim36/Haprin during Xenopus laevis early embryogenesis and its involvement in somite formation. Temporal expression analysis indicated that Trim36/Haprin was present throughout embryogenesis. Spatial expression analysis showed that its expression was mainly confined to the nervous system and a portion of the posterior somite. Morpholino-mediated knockdown of Trim36/Haprin markedly and specifically inhibited the somite formation. We conclude that Trim36/Haprin plays an important role in the arrangement of somites during their formation.
Yoshigai
Emi
E
Graduate School of Genetic Resources Technology, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Kawamura
Shinobu
S
Kuhara
Satoru
S
Tashiro
Kosuke
K
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D055785:Gene Knockdown Techniques; D047908:Intracellular Signaling Peptides and Proteins; D016376:Oligonucleotides, Antisense / Q000235:genetics; D019170:Somites / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D000070584:Tripartite Motif Proteins
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D016376:Oligonucleotides, Antisense; D029867:Xenopus Proteins; C534481:Trim36 protein, Xenopus; D044767:Ubiquitin-Protein Ligases; D000070584:Tripartite Motif Proteins