Proteome patterns in uremic plasma.
In patients with chronic kidney disease (CKD), peptides and proteins circulate at altered concentrations versus in healthy individuals. We have characterized proteome samples from 7 pooled CKD stage 5 patients not yet on dialysis and with no known co-morbidities. We also analyzed pooled plasma samples from 7 healthy age- and sex-matched controls. After immunodepletion of the 6 most abundant plasma proteins, HPLC and SDS-PAGE patterns differed between the healthy and disease groups. The differing proteins were identified by peptide mass fingerprinting using MALDI mass spectrometry and verified with electrospray tandem mass spectrometry sequence analysis. Multiple differences in at least 19 HPLC fractions were observed, from which we identified 29 proteins, 25 in greater yield and 4 in lower yield than in the healthy controls, adding at least 6 protein components to those that were previously known to be altered in CKD.
Naseeb
Uzma
U
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Shafqat
Jawed
J
Jägerbrink
Theres
T
Zarina
Shamshad
S
Alvestrand
Anders
A
Jörnvall
Hans
H
Axelsson
Jonas
J
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000097:blood; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014511:Uremia
D001798:Blood Proteins