title: DC-SIGN and DC-SIGNR polymorphic variants in Northern Asian Indians.
abstract: Dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein (DC-SIGNR), along with DC-SIGN, is suggested to facilitate HIV infection of T cells in trans through binding with HIV gp120. We studied the repeat region polymorphisms in DC-SIGN and DC-SIGNR in 100 healthy HIV-1 seronegative individuals among Northern Asian Indians. Each variant polymorphism obtained by polymerase chain reaction (PCR) was confirmed by cloning and sequencing. Fifty-four per cent of the healthy seronegative individuals were homozygous for the DC-SIGNR 7/7 repeat. The heterozygous 7/5 variant was found in 25%, while the 5/5 homozygous genotype was found in 17% of the subjects. Allele 8 was rare and accounted for 4% of the heterozygous genotype (8/7) in the sample population. DC-SIGN polymorphism was rare, and the genotype 7/7 was most frequent in this study population. Further studies are warranted in a large sample size including high-risk and seropositive HIV patients to confirm the association of DC-SIGNR polymorphisms with HIV-1 susceptibility.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: O
authors.initials: O
authors.lastname: Chaudhary
authors.forename: R
authors.initials: R
authors.lastname: Bhasin
authors.forename: K
authors.initials: K
authors.lastname: Luthra
mesh_terms: D000483:Alleles; D015815:Cell Adhesion Molecules / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1; D006801:Humans; D007194:India; D037181:Lectins, C-Type / Q000235:genetics*; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C433029:CLEC4M protein, human