title: Effect of temperature up-shift on fermentation and metabolic characteristics in view of gene expressions in Escherichia coli.
abstract: BACKGROUND
  Escherichia coli induces heat shock genes to the temperature up-shift, and changes the metabolism by complicated mechanism. The heat shock response is of practical importance for the variety of applications such as temperature-induced heterologous protein production, simultaneous saccharification and fermentation (SSF) etc. However, the effect of heat shock on the metabolic regulation is not well investigated. It is strongly desired to understand the metabolic changes and its mechanism upon heat shock in practice for the efficient metabolite production by temperature up-shift. In the present research, therefore, we investigated the effect of temperature up-shift from 37 degrees C to 42 degrees C on the metabolism in view of gene expressions.
  
  
  RESULTS
  The results of aerobic batch and continuous cultivations of E. coli BW25113 indicate that more acetate was accumulated with lower biomass yield and less glucose consumption rate at 42 degrees C as compared to the case at 37 degrees C. The down- regulation of the glucose uptake rate corresponds to the down-regulation of ptsG gene expression caused by the up-regulation of mlc gene expression. In accordance with up-regulation of arcA, which may be caused by the lower oxygen solubility at 42 degrees C, the expressions of the TCA cycle-related genes and the respiratory chain gene cyoA were down-regulated. The decreased activity of TCA cycle caused more acetate formation at higher temperature, which is not preferred in heterologous protein production etc. This can be overcome by the arcA gene knockout to some extent. The time courses of gene expressions revealed that the heat shock genes such as groEL, dnaK, htpG and ibpB as well as mlc were expressed in much the same way as that of rpoH during the first 10-20 minutes after temperature up-shift. Under microaerobic condition, the fermentation changed in such a way that formate and lactate were more produced due to up-regulation of pflA and ldhA genes while ethanol was less produced due to down-regulation of adhE gene at higher temperature as compared to the case at 37 degrees C.
  
  
  CONCLUSIONS
  The present result clarified the mechanism of metabolic changes upon heat shock from 37 degrees C to 42 degrees C based on gene expressions of heat shock genes, global regulators, and the metabolic pathway genes. It is recommended to use arcA gene knockout mutant to prevent higher acetate production upon heat shock, where it must be noted that the cell yield may be decreased due to TCA cycle activation by arcA gene knockout.
authors.affiliation: Department of Bioscience & Bioinformatics, Kyushu Institute of Technology, Iizuka, Fakuoka 820-8502, Japan. shimi@bio.kyutech.ac.jp.
authors.forename: Chowdhurry Mohammad Monirul
authors.initials: CM
authors.lastname: Hasan
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Shimizu