Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene.
We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to approximately 1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.
Gunn
Shelly R
SR
Department of Pathology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Bolla
Aswani R
AR
Barron
Lynn L
LL
Gorre
Mercedes E
ME
Mohammed
Mansoor S
MS
Bahler
David W
DW
Mellink
Clemens H M
CH
van Oers
Marinus H J
MH
Keating
Michael J
MJ
Ferrajoli
Alessandra
A
Coombes
Kevin R
KR
Abruzzo
Lynne V
LV
Robetorye
Ryan S
RS
D000483:Alleles; D000951:Antigens, Neoplasm / Q000235:genetics*; D002872:Chromosome Deletion; D002892:Chromosomes, Human, Pair 22; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D000951:Antigens, Neoplasm; C404944:PRAME protein, human