Evaluation of an immunochromatographic assay kit for rapid identification of Mycobacterium tuberculosis complex in clinical isolates.
We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.
Park
Mi Young
MY
Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea.
Kim
Young Jin
YJ
Hwang
Sang Hyun
SH
Kim
Hyoung Hoi
HH
Lee
Eun Yup
EY
Jeong
Seok Hoon
SH
Chang
Chulhun L
CL
D000818:Animals; D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D000911:Antibodies, Monoclonal