title: Studies on the nature of hemolytic effect induced by ethylene glycol alkyl ethers.
abstract: The nature of hemolytic effect induced by ethylene glycol alkyl ethers was analyzed taking into account G-6-PDH activity, ATP, pyruvate and thiols levels in peripheral blood of rats treated with single doses of 2-ethoxyethanol and 2-butoxyethanol. In addition, the susceptibility to autoxidation of rat erythrocyte lipids was evaluated. A decrease of ATP level in a dose-dependent manner and an increase in protein- and nonprotein-bound sulfhydryl groups were observed. These results indicate that an acute hemolytic effect of ethylene glycol alkyl ethers is not associated with alterations in G-6-PDH activity and the susceptibility of erythrocyte lipids to autoxidation. Increases in protein- and nonprotein-bound sulfhydryl groups seem to indicate the selective hemolysis of the aged erythrocytes. The increase in pyruvate and thiol levels may protect erythrocytes against the appearance of oxidative stress.
authors.affiliation: Department of Biochemical Toxicology, Medical College, Jagiellonian University, 9 Medyczna Str. Kraków, Poland. mfstarek@cyf-kr.edu.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Starek
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005026:Ethylene Glycols / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005026:Ethylene Glycols; D008315:Malondialdehyde; C017096:n-butoxyethanol; C021296:2-ethoxyethanol