DNA loaded carrier preferential extravasation from tumor blood vessel.
Non-viral gene delivery carriers were prepared by using DNA/polyethylenimine/polymethacrylic acid (DPP) polyplexes and its extravasation from tumor blood vessel was evaluated with mouse dorsal skin fold window chamber model. The DNA/PEI (DP) complex with a ratio of N to P (10/1) was coated with polymethacrylic acid, and the ratio of PMA to DNA complex in DNA/PEI/PMA (DPP) polyplex was fixed 0.03 (w/w). The surface charges of the DP and DPP polyplex were positive 26 and 15, respectively. The size of DP and DPP polyplex were 161nm and 195nm. The transfection efficiencies in HepG2 cells were about 30-fold and 20-fold higher than that in HeLa and L/C cells in the presence of 50% serum, respectively. The DPP polyplex showed a reduced erythrocyte aggregation activity and a decreased cytotoxicity in cancer cells. After being incubated 30min, Fluorescently labelled DPP polyplex uptaken by cancer cells decreased, compared with DP by measuring flowcytometry. DPP polyplex penetrating through tumor blood vessel appeared fast and stayed longer in tumour interstitial, this fact was observed from mouse dorsal skin fold window chamber model.
Jiang
Ge
G
Key Laboratory of Bio-organic Chemistry and College of Bioengineering, Dalian University, Dalian 116622, China.
Jiang
Yingzhi
Y
Shen
Yuanyuan
Y
Nam
Kweon-Ho
KH
Lee
Donhaeng
D
Gao
Zhonggao
Z
D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage*; D004903:Erythrocyte Aggregation; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000098:blood supply; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012884:Skin Window Technique; D014162:Transfection / Q000379:methods
D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D011094:Polyethyleneimine; D004247:DNA