Difference between the abilities of human Fcgamma receptor-expressing CV-1 cells to neutralize American and Asian genotypes of dengue virus 2.
Sera from patients involved in a Peruvian outbreak of dengue virus serotype 1 infection cross-neutralized the American genotype of dengue virus serotype 2 up to 100-fold more efficiently than they did the virulent Asian genotype of dengue virus serotype 2, as determined by a plaque reduction neutralization test (PRNT) with CV-1 fibroblasts modified to express human Fcgamma receptor CD32. The concordant preferential immune enhancement of the Asian genotype of dengue virus serotype 2 in human monocytes suggests that such a modification might strengthen the correlation between the PRNT titer and protection.
Rodrigo
W W Shanaka I
WW
Department of Pathology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
Rodrigo
W W I S
WW
Alcena
D C
DC
Kou
Z
Z
Kochel
T J
TJ
Porter
K R
KR
Comach
G
G
Rose
R C
RC
Jin
X
X
Schlesinger
J J
JJ
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D005838:Genotype; D006801:Humans; D009500:Neutralization Tests; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D017452:Receptors, IgG