Real-time PCR monitoring of signal transduction related genes involved in water stress tolerance mechanism of sunflower.
The study deals with the quantitative expression pattern of genes involved in signaling transduction pathways in response to water stress in leaves and embryos of a water stress tolerant genotype compared to a non-tolerant genotype using real-time quantitative PCR. The experiment was conducted in the field. The results showed a high quantitative up-regulation of genes belonging to protein kinase, phosphatase and transcription factor pathways (from two to 70 fold) only in leaves of the tolerant genotype compared to the non-tolerant genotype. Moreover, genes related to the protein kinase pathway were down-regulated in leaves of the non-tolerant genotype. On the contrary, in seeds, our study showed that the positive regulation of genes related to the signal transduction pathway observed in leaves of the tolerant genotype is turned off, suggesting different transcriptional control of signaling water stress in reproductive organs compared to vegetative organs.
Roche
Jane
J
UMR 1248 France INRA/ENSAT Centre de Toulouse, Chemin de Borde Rouge, BP 27 31326, Auzeville, Castanet-Tolosan, France. jane.roche@univ-bpclermont.fr
Hewezi
Tarek
T
Bouniols
Andrée
A
Gentzbittel
Laurent
L
D000222:Adaptation, Physiological / Q000235:genetics*; D003681:Dehydration / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D014644:Genetic Variation; D005838:Genotype; D006368:Helianthus / Q000235:genetics* / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011494:Protein Kinases / Q000235:genetics; D012639:Seeds / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D015854:Up-Regulation
D014157:Transcription Factors; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases