Analysis of flavonoids in propolis and Ginkgo biloba by micellar electrokinetic capillary chromatography.
A micellar electrokinetic capillary chromatography (MEKC) method has been developed for simultaneous determination of 10 bioactive flavonoids: rutin, apigenin, luteolin, eriodictyol, kaempferol, chrysin, acacetin, flavanone, flavone, and fisetin. The effect of several parameters, such as UV detection wavelength, buffer pH, buffer concentration, sodium dodecyl sulfate (SDS) concentration, beta-cyclodextrin (beta-CD) concentration, separation voltage, and injection time on the separation of these flavonoids were systematically investigated. The 10 flavonoids were successfully separated within 18 min in 20 mM Na(2)B(4)O(7)-10 mM NaH(2)PO(4) buffer (pH 9.7) containing 100 mM SDS and 16 mM beta-CD at a separation voltage of 19 kV, with UV detection at 254 nm. Regression analysis revealed a good linear relationship between the peak area of each analyte and its concentration with detection limits (S/N = 3), ranging from 0.15 to 1.36 microg mL(-1). This method could simultaneously quantify the 10 flavonoids and be used in the quality control of functional foods containing propolis and Ginkgo biloba.
Jiang
Liqin
L
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China.
Fang
Guozhen
G
Zhang
Yan
Y
Cao
Guojie
G
Wang
Shuo
S
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D020441:Ginkgo biloba / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry*
D005419:Flavonoids; D011429:Propolis