title: Candida kanchanaburiensis sp. nov., a new ascomycetous yeast species related to Pichia nakazawae isolated in Thailand.
abstract: Three yeast strains, ST-633, ST-634 and ST-635, isolated from the fruit body of a mushroom, Coprinus sp., and rotted fruit of guava collected in the western region of Thailand, were found to represent a hitherto undescribed species. This yeast is related to Pichia nakazawae var. akitaensis, P. nakazawae var. nakazawae and Pichia philogaea in the D1/D2 domain of 26S rDNA but 12 (2.3%), 13 (2.5%) and 15 (2.8%) nucleotides are different from these taxa, respectively, suggesting the distinctness of the Thai strains at species level. Since ascospore formation was not detected, it is described as a new species of Candida, Candida kanchanaburiensis. This species is distinguished from P. nakazawae by the ability to assimilate 2-ketogluconic acid and L-lysine, and inability to assimilate soluble starch.
authors.affiliation: NITE Biological Resource Center, Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, Japan nakase-takahashi@nite-go.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakase
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Jindamorakot
authors.forename: Shinya
authors.initials: S
authors.lastname: Ninomiya
authors.forename: Yumi
authors.initials: Y
authors.lastname: Imanishi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Wanchern
authors.initials: W
authors.lastname: Potacharoen
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003302:Coprinus; D004271:DNA, Fungal / Q000032:analysis; D005942:Gluconates / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010641:Phenotype; D010802:Phylogeny; D010843:Pichia / Q000145:classification* / Q000235:genetics; D031647:Psidium / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D005942:Gluconates; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; C008229:2-ketogluconate; D008239:Lysine