Rice aldehyde dehydrogenase7 is needed for seed maturation and viability.
Aldehyde dehydrogenases (ALDHs) catalyze the irreversible oxidation of a wide range of reactive aldehydes to their corresponding carboxylic acids. Although the proteins have been studied from various organisms and at different growth stages, their roles in seed development have not been well elucidated. We obtained T-DNA insertional mutants in OsALDH7, which is remarkably inducible by oxidative and abiotic stresses. Interestingly, endosperms from the osaldh7 null mutants accumulated brown pigments during desiccation and storage. Extracts from the mutant seeds showed a maximum absorbance peak at 360 nm, the wavelength that melanoidin absorbs. Under UV light, those extracts also exhibited much stronger fluorescence than the wild type, suggesting that the pigments are melanoidin. These pigments started to accumulate in the late seed developmental stage, the time when OsALDH7 expression began to increase significantly. Purified OsALDH7 protein showed enzyme activities to malondialdehyde, acetaldehyde, and glyceraldehyde. These results suggest that OsALDH7 is involved in removing various aldehydes formed by oxidative stress during seed desiccation. The mutant seeds were more sensitive to our accelerated aging treatment and accumulated more malondialdehyde than the wild type. These data imply that OsALDH7 plays an important role in maintaining seed viability by detoxifying the aldehydes generated by lipid peroxidation.
Shin
Jun-Hye
JH
National Research Laboratory, Department of Integrative Bioscience and Biotechnology, and Center for Functional Genomics, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Kim
Sung-Ryul
SR
An
Gynheung
G
D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007700:Kinetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000502:physiology*; D013379:Substrate Specificity
D004269:DNA, Bacterial; D010940:Plant Proteins; C036483:T-DNA; D000444:Aldehyde Dehydrogenase