Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro.
The present study aimed to evaluate whether the association between a calcium hydroxide paste (Calen paste) and 0.4% chlorhexidine (CHX) affects the development of the osteogenic phenotype in vitro. With rat calvarial osteogenic cell cultures, the following parameters were assayed: cell morphology and viability, alkaline phosphatase activity, total protein content, bone sialoprotein immunolocalization, and mineralized nodule formation. Comparisons were carried out by using the nonparametric Kruskal-Wallis test (level of significance, 5%). The results showed that the association between Calen paste and 0.4% CHX did not affect the development of the osteogenic phenotype. No significant changes were observed in terms of cell shape, cell viability, alkaline phosphatase activity, and the total amount of bone-like nodule formation among control, Calen, or Calen + CHX groups. The strategy to combine Ca(OH)(2) and CHX to promote a desirable synergistic antibacterial effect during endodontic treatment in vivo might not significantly affect osteoblastic cell biology.
da Silva
Raquel Assed Bezerra
RA
Department of Pediatric Clinic, Preventive and Social Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. raquel@forp.usp.br
Leonardo
Mário Roberto
MR
da Silva
Léa Assed Bezerra
LA
de Castro
Larissa Moreira Spinola
LM
Rosa
Adalberto Luiz
AL
de Oliveira
Paulo Tambasco
PT
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001857:Bone Matrix / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002126:Calcium Hydroxide / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002710:Chlorhexidine / Q000494:pharmacology*; D004396:Coloring Agents; D004338:Drug Combinations; D005456:Fluorescent Dyes; D007211:Indoles; D058574:Integrin-Binding Sialoprotein; D009930:Organic Chemicals; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000032:analysis; D013778:Tetrazolium Salts; D013844:Thiazoles
C417664:Alexa594; D004396:Coloring Agents; D004338:Drug Combinations; D005456:Fluorescent Dyes; C546873:Ibsp protein, rat; D007211:Indoles; D058574:Integrin-Binding Sialoprotein; D009930:Organic Chemicals; D011506:Proteins; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D012795:Sialoglycoproteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C420643:calen; D053495:Osteopontin; C007293:DAPI; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D002126:Calcium Hydroxide; D002710:Chlorhexidine