Combination of CCR1 mRNA, MCP1, and CA125 measurements in peripheral blood as a diagnostic test for endometriosis.
This study investigated the possible use of CCR1 mRNA measurement in peripheral blood leukocytes in combination with measurements of monocyte chemotactic protein-1 (MCP-1) and CA125 protein in serum as a diagnostic test for endometriosis.The expression of CCR1 mRNA in peripheral blood leukocytes was measured by quantitative real-time polymerase chain reaction. MCP-1 and CA125 levels in serum were determined by ELISA and ECLIA.The ratio of CCR1/HPRT mRNA in peripheral blood of patients with endometriosis and adenomyosis was significantly elevated compared with women without endometriosis. Additionally, serum levels of MCP-1 and CA125 were significantly higher in patients with endometriosis. This method showed a sensitivity of 92.2%, a specificity of 81.6%, a negative predictive value of 83.3%, a positive predictive value of 92.3%, a likelihood ratio of a positive test result of 5.017, and a likelihood ratio of a negative test result of 0.096 to predict the presence or absence of endometriosis.The results imply the potential use of CCR1 mRNA, MCP-1, and CA125 protein measurements for the diagnosis or exclusion of endometriosis.
Agic
Admir
A
Department of Gynecology and Obstetrics, University of Schleswig-Holstein, Luebeck, Germany.
Djalali
Schima
S
Wolfler
Monika M
MM
Halis
Gulden
G
Diedrich
Klaus
K
Hornung
Daniela
D
D018394:CA-125 Antigen / Q000097:blood*; D018932:Chemokine CCL2 / Q000097:blood*; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D012333:RNA, Messenger / Q000097:blood; D054389:Receptors, CCR1 / Q000097:blood* / Q000235:genetics; D012189:Retrospective Studies
D018394:CA-125 Antigen; C111835:CCL2 protein, human; C515300:CCR1 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger; D054389:Receptors, CCR1