title: Molecular cloning and sequence analysis of the sisomicin biosynthetic gene cluster from Micromonospora inyoensis.
abstract: Micromonospora inyoensis produces sisomicin (Sm), an aminoglycoside antibiotic. The gene cluster of sisomicin biosynthesis spanning ca. 47 kb consists of 37 ORFs encoding various proteins for sisomicin biosynthesis, regulation, resistance and transport. The comparative genetic studies on the biosynthetic genes of sisomicin and gentamicin (Gm) reveal a similar biosynthetic route and provide a framework for the future biosynthetic studies.
authors.affiliation: College of Biological Science and Technology, Fuzhou University, 523 Gongye Rd, Fuzhou City, Fujian, China.
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Hong
authors.forename: Wun-Rong
authors.initials: WR
authors.lastname: Hong
authors.forename: Mei
authors.initials: M
authors.lastname: Ge
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Zeng
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Min-Yu
authors.initials: MY
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Shao
authors.forename: Dai-Jie
authors.initials: DJ
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D023061:Gene Order; D008847:Micromonospora / Q000235:genetics*; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D012853:Sisomicin / Q000096:biosynthesis*; D026801:Synteny
chemical_list: D000900:Anti-Bacterial Agents; D012853:Sisomicin