title: Strain difference in susceptibility to Trichinella spiralis infection between dark agouti and albino oxford rats.
abstract: The influence of host genetics on the susceptibility to primary Trichinella spiralis infection has been extensively studied in a mouse model, but has not been clarified for rats. Analyses of interstrain and intrastrain genetic variation in response to infectious agents could be beneficial not only for elucidating the genetic basis of host resistance/susceptibility, but for revealing immune response mechanisms as well. The aim of this study was to analyse interstrain differences in worm burdens and cytokine production between Albino Oxford (AO) and Dark Agouti (DA) rats in muscle phase of T. spiralis infection. Clear strain-dependent variation was observed in the number of T. spiralis larvae per gram (lpg) of muscle tissue where values for DA rats (626.7+/-171lpg) vastly exceeded those found in AO rats (49.8+/-25.9lpg, p<0.001). Differences between the strains were also noticed in key cytokine levels. In infected AO rats, the cytokine production remained in favor of Th1 type response, while infected DA rats showed a shift towards a Th2 type response. The level of regulatory IL-10 was significantly increased only in T. spiralis infected DA rats. Our results provide evidence that DA rats express higher susceptibility to T. spiralis infection in comparison to AO rats with respect to muscle larvae burden. The infection in DA rats was accompanied by the production of anti-inflammatory cytokines, while the response of AO rats was characterized by a proinflammatory type of immune response.
authors.affiliation: Institute for the Application of Nuclear Energy, University of Belgrade, Banatska 31b, Belgrade, Serbia. svasilev@inep.co.yu
authors.forename: S
authors.initials: S
authors.lastname: Vasilev
authors.forename: A
authors.initials: A
authors.lastname: Gruden-Movsesijan
authors.forename: N
authors.initials: N
authors.lastname: Ilic
authors.forename: L J
authors.initials: LJ
authors.lastname: Sofronic-Milosavljevic
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D020022:Genetic Predisposition to Disease; D007814:Larva; D018482:Muscle, Skeletal / Q000378:metabolism / Q000469:parasitology; D051381:Rats; D017160:Trichinella spiralis / Q000502:physiology*; D014235:Trichinellosis / Q000235:genetics*
chemical_list: D016207:Cytokines