Detection of an antibody to avian influenza virus by an electrochemical immunoassay (eELISA).
The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10 - 1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.
Ohtsuka
Keiichi
K
Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan.
Endo
Hiroshi
H
Morimoto
Kimio
K
Vuong
Bui N
BN
Ogawa
Haruko
H
Imai
Kunitoshi
K
Takenaka
Shigeori
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D004209:Disposable Equipment; D004563:Electrochemistry; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005240:Feasibility Studies; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000378:metabolism
D000814:Aniline Compounds; D000914:Antibodies, Viral; D009943:Organophosphorus Compounds; C068798:4-aminophenylphosphate; D000469:Alkaline Phosphatase