Application of biotransformation in flavor and fragrance industry.
The present study describes the isolation of microorganisms capable of producing alpha-pinene from beta-pinene. 24 (15 fungi, 9 bacteria) microorganisms were isolated from galbanum, gum and soil, were screened for their ability to transform beta-pinene to alpha-pinene. Biotransformation products were extracted with n-hexan and analyzed by gas chromatography. One microorganism (bacterial strain) were found. The biotransformation medium involved, phosphate buffer pH 6, 100 microL beta-pinene, 1 g biomass of microorganism, 37 degrees C, 150 rpm and 22 h. The experiments were performed in conical flasks. The optimum cell growth were obtained when 30 g L(-1) glycerin applied. The optimum conversion beta-pinene to alpha-pinene was obtained when 20 g L(-1) glycerin applied as carbon source for bacterial strain.
Kashi
Fereshteh Jookar
FJ
Department of Biology, School of Science, Al-Zahra University, Vanak, Tehran, Iran.
Fooladi
Jamshid
J
Bayat
Mansour
M
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D001711:Biotransformation; D002849:Chromatography, Gas; D003470:Culture Media; D005421:Flavoring Agents / Q000493:pharmacokinetics*; D010476:Perfume / Q000493:pharmacokinetics*
D003470:Culture Media; D005421:Flavoring Agents; D010476:Perfume