Species identification of Campylobacter jejuni ssp. jejuni and C. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and PCR.
The Campylobacter species strains (n = 42; isolated from clinical samples and deposited in Czech National Collection of Type Cultures, Prague) originally phenotypically (and biochemically) identified as Campylobacter jejuni were re-classified using molecular biological and mass spectrometric methods. Whole-cell MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) separated the isolates into two genetically related strains--C. jejuni (n = 26) and C. coli (n = 16) and, moreover, distinguished the intimate details in the group of tested strains. It also made it possible to create the MALDI-TOF MS dendrogram; similarly, the spectral characteristics were used for the 3D cluster analysis. Polymerase chain reaction (PCR) confirmed the results obtained by mass spectrometry. Both methods (PCR and MALDI-TOF MS) gave the same results which supports their suitability in the rapid and accurate Campylobacter-species determination.
Kolínská
R
R
Czech National Collection of Type Cultures, National Institute of Public Health, 100 42, Prague, Czech Republic.
Drevínek
M
M
Jakubů
V
V
Zemlicková
H
H
D000818:Animals; D015373:Bacterial Typing Techniques; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics; D002415:Cats; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D004269:DNA, Bacterial