title: [Effects of constant magnetic fields on proliferation and migration of endothelial progenitor cells under rapamycin intervention: experiment with rats].
abstract: OBJECTIVE
  To investigate the effects of constant magnetic field (CMF) on proliferation and migration of bone marrow-derived endothelial progenitor cells (EPCs) under rapamycin intervention.
  
  
  METHODS
  EPCs were isolated from rat bone marrow by density gradient centrifugation and cultured on fibronectin-coated dishes. Six days later the attached cells were divided into 5 groups: control group, rapamycin (1 ng/ml) group, and 3 rapamycin + CMF groups (treated with CMF of the doses 0.1 mT, 0.5 mT, and 1.0 mT respectively). Samples were collected 24 hours after incubation. Cell proliferation was measured by MTT chromatometre. EPC migration was detected with modified Boyden chamber assay.
  
  
  RESULTS
  The EPC proliferation ability of the rapamycin group, expressed by absorbance, was (0.252 +/- 0.006), significantly lower than that of the control group [(0.328 +/- 0.025), P < 0.05]. The number of migrating EPC was (31 +/- 3) cells, significantly lower than that of the control group [(48 +/- 5), P < 0.05]. The EPC proliferation ability of the rapamycin + CMF 0.5 mT and 1.0 mT groups, expressed by absorbance, were (0.278 +/- 0.008) and (0.280 +/- 0.010) respectively, both significantly higher than that of the control group (both P < 0.05). The migrating EPC number of the rapamycin + CMF 0.5 mT and 1.0 mT groups were (37 +/- 3) and (38 +/- 4) respectively, both significantly higher than that of the control group (both P < 0.05).
  
  
  CONCLUSIONS
  CMF of the doses of 0.5 mT and 1.0 mT antagonizes the effects of rapamycin on EPCs, increasing the proliferation and migration of EPCs.
authors.affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: He-xiang
authors.initials: HX
authors.lastname: Cheng
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Xu
authors.forename: Rong-qing
authors.initials: RQ
authors.lastname: Zhang
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Luan
authors.forename: Wen-yi
authors.initials: WY
authors.lastname: Guo
authors.forename: Hai-chang
authors.initials: HC
authors.lastname: Wang
authors.forename: Zhen-tao
authors.initials: ZT
authors.lastname: Yu
authors.forename: Lian
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion / Q000528:radiation effects; D002454:Cell Differentiation; D002465:Cell Movement / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020123:Sirolimus / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*
chemical_list: D020123:Sirolimus