Immunohistochemical and proteomic profile of melanotic medulloblastoma.
We present the case of a 6-year-old male affected by an infratentorial tumor. Histological diagnosis was melanotic medulloblastoma. Immunohistochemistry showed in the melanin rich areas positive cells for HMB45. We performed a proteomic study to compare protein profiles in melanotic versus non-melanotic areas. Protein profiles of different areas of the tumor displayed similarity, with the exception of seven proteins. In accordance with the hypothesis that melanotic medulloblastomas produce oculo-cutaneous melanin, proteomic analysis showed melanocytic-associated antigens and epidermal autoantigen 450K in the pigmented nodule; both these proteins have a significant role as markers of melanotic elements.
Zanini
Cristina
C
Department of Genetics, Biology and Biochemistry, University of Turin, Turin, Italy. cristina.zanini@unito.it
Mandili
Giorgia
G
Pulerà
Francesco
F
Morra
Isabella
I
Peretta
Paola
P
Turrini
Franco
F
Forni
Marco
M
D002528:Cerebellar Neoplasms / Q000378:metabolism* / Q000473:pathology; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D013058:Mass Spectrometry; D008527:Medulloblastoma / Q000378:metabolism* / Q000473:pathology; D008543:Melanins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D040901:Proteomics
D008543:Melanins; D009363:Neoplasm Proteins