Effect of MT1-MMP deficiency and overexpression in corneal keratocytes on vascular endothelial cell migration and proliferation.
OBJECTIVE
To determine the effect of keratocyte-derived MT1-MMP on calf pulmonary artery endothelial cell (CPAE) proliferation and migration.


METHODS
Keratocyte lines were generated from MT1-MMP knockout (KO) and wild type (WT) mice. WT keratocytes were transfected with WT or mutant MT1-MMP DNAs (DeltaTC or E240A). The effect of keratocyte-conditioned media on CPAE proliferation and migration was assayed.


RESULTS
KO keratocyte conditioned media resulted in the greatest increase of CPAE cell proliferation (190.5+/-6.0%; p<0.01). WT keratocyte conditioned media showed higher CPAE proliferation (155.4+/-3.6%) than WT/MT1-MMP-transfected keratocytes (119.7+/-2.2%; p<0.001). Migration assays confirmed these findings.


CONCLUSIONS
Keratocyte-derived MT1-MMP has anti-angiogenic effects in CPAE cells.
Azar
Dimitri T
DT
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois 60612, USA. dazar@uic.edu
Casanova
Fabio H
FH
Mimura
Tatsuya
T
Jain
Sandeep
S
Chang
Jin-Hong
JH
D020533:Angiogenesis Inhibitors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000201:enzymology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D053511:Matrix Metalloproteinase 14 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D011651:Pulmonary Artery / Q000166:cytology*; D014162:Transfection; D015854:Up-Regulation
D020533:Angiogenesis Inhibitors; D017077:Culture Media, Conditioned; D053511:Matrix Metalloproteinase 14