Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles.
Nanoparticle size and plasma binding profile contribute to a particle's longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.
Dobrovolskaia
Marina A
MA
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick Inc., NCI-Frederick, Frederick, Maryland 21702, USA. marina@mail.nih.gov
Patri
Anil K
AK
Zheng
Jiwen
J
Clogston
Jeffrey D
JD
Ayub
Nader
N
Aggarwal
Parag
P
Neun
Barry W
BW
Hall
Jennifer B
JB
McNeil
Scott E
SE
D001665:Binding Sites; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008854:Microscopy, Electron; D020527:Microscopy, Scanning Probe; D010316:Particle Size
D001798:Blood Proteins; D006046:Gold