Characterization of neutralizing epitopes of varicella-zoster virus glycoprotein H.
Varicella-zoster virus (VZV) glycoprotein H (gH) is the major neutralization target of VZV, and its neutralizing epitope is conformational. Ten neutralizing human monoclonal antibodies to gH were used to map the epitopes by immunohistochemical analysis and were categorized into seven epitope groups. The combinational neutralization efficacy of two epitope groups was not synergistic. Each epitope was partially or completely resistant to concanavalin A blocking of the glycomoiety of gH, and their antibodies inhibited the cell-to-cell spread of infection. The neutralization epitope comprised at least seven independent protein portions of gH that served as the target to inhibit cell-to-cell spread.
Akahori
Yasushi
Y
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
Suzuki
Kazuhiro
K
Daikoku
Tohru
T
Iwai
Masae
M
Yoshida
Yoshihiro
Y
Asano
Yoshizo
Y
Kurosawa
Yoshikazu
Y
Shiraki
Kimiyasu
K
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D008562:Membrane Glycoproteins; D014764:Viral Proteins; C040496:gp 118 protein, Herpesvirus 3, Human