[Association between single nucleotide polymorphisms in the promoter of cyclooxygenase COX-2 gene and hereditary susceptibility to pancreatic cancer].
OBJECTIVE
To evaluate the effects of -1290A > G, -1195G > A and -765G > C single nucleotide polymorphisms (SNPs) in the promoter of cyclooxygenase (COX)-2 gene on the risk of pathogenesis of pancreatic cancer.


METHODS
Peripheral blood samples were collected from 283 patients with pancreatic cancer and 566 normal controls. Questionnaire survey was conducted to understand the demographic data and status of smoking and smoking cessation of the subjects. Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of the gene fragments containing the 3 SNP sites in the promoter regions of the COX-2 gene. Statistical tests were performed to analyze the relations among different factors and the risk of pancreatic cancer.


RESULTS
Three SNPs, -1290A > G, -1195G > A, and -765G > C were identified. A case-control analysis revealed 1.75-fold (95% CI = 1.16-2.64) and 2.53-fold (95% CI = 1.43-4.47) excesses of risks of developing pancreatic cancer for the -1195AA and -765CG genotype carriers respectively compared with the non-carriers. Compared with A(-1290)-G(-1195)-G(-765) containing haplotype, greater risks of developing pancreatic cancer were observed for A(-1290)-A(-1195)-G(-765), (OR = 1.26, 95%, CI = 1.02-1.56) and G(-1290)-A(-1195)-C(-765) (OR = 5.54, 95% CI = 1.79-17.16) containing haplotypes. There were interactions between the -765CG or -1195AA genotype and smoking in the risk of developing pancreatic cancer.


CONCLUSIONS
The SNP of -1195A > G and -765G > C in the COX -2 promoter may play an important role in mediating hereditary susceptibility to developing pancreatic cancer.
Xu
Dong-kui
DK
Department of Cancer Surgery, First Municipal Hospital of Xiamen Affiliated to Fujian Medical University, Xiamen 361000, China.
Zhang
Xue-mei
XM
Zhao
Ping
P
Cai
Jian-chun
JC
Zhao
Dan
D
Tan
Wen
W
Guo
Yong-li
YL
Lin
Dong-xin
DX
D000368:Aged; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012907:Smoking; D011795:Surveys and Questionnaires
D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human