Serum total homocysteine, folate, 5,10-methylenetetrahydrofolate reductase (MTHFR) 677C-->T genotype and subclinical atherosclerosis.
OBJECTIVE
To determine the relationship of serum total homocysteine (tHcy), serum folate and 5,10-methylenetetrahydrofolate reductase (MTHFR) 677C-->T genotype with ultrasonic arterial wall measurements associated with subclinical atherosclerosis.


METHODS
Cross-sectional analysis of 767 participants in an ongoing prospective study. Intima-media thickness (IMT) of the common carotid (IMTcc), IMT of the internal carotid including plaque when present (IMT(max)) and the sum of the thickest plaques present in both carotid and both common femoral bifurcations (total plaque thickness (TPT)) were measured using ultrasound.


RESULTS
People in the upper homocysteine quartile were more likely to have clinical cardiovascular disease (CVD) than those in the lowest three quartiles. They were also more likely to have plaques. The MTHFR 677C-->T genotype was not associated with any of the measures of subclinical atherosclerosis in either men or women but was the most important determinant of total homocysteine levels in men under 60 years of age.


CONCLUSIONS
Increased homocysteine levels but not MTHFR 677C-->T genotype, are associated with subclinical atherosclerosis and the presence of plaques. Our results indicate that measurements of blood levels of homocysteine and folate in people at intermediate risk for atherosclerotic CVD before symptoms occur, might improve risk stratification and facilitate the decision to provide folate/B vitamin intervention in primary prevention.
Panayiotou
Andrie
A
University of Cyprus, Department of Biological Sciences, PO Box 20537, 1678, Nicosia, Cyprus. bipgap1@ucy.ac.cy
Nicolaides
Andrew
A
Griffin
Maura
M
Tyllis
Theodosis
T
Georgiou
Niki
N
Martin
Richard M
RM
Bond
Dawn
D
Tziakouri-Shiakalli
Chrysa
C
Fessas
Charis
C
Deltas
Constantinos
C
D000368:Aged; D050197:Atherosclerosis / Q000097:blood* / Q000235:genetics; D001483:Base Sequence; D003430:Cross-Sectional Studies; D017931:DNA Primers; D005260:Female; D005492:Folic Acid / Q000097:blood*; D005838:Genotype; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000097:blood* / Q000235:genetics; D008875:Middle Aged
D017931:DNA Primers; D006710:Homocysteine; D005492:Folic Acid; D042965:Methylenetetrahydrofolate Reductase (NADPH2)