title: [Expression feature and bioinformatic analysis of molar root patterning gene 1].
abstract: OBJECTIVE
  To examine the expression of molar root patterning gene 1 (Mrp1) and predict the Mrp1 structure by bioinformatics analysis.
  
  
  METHODS
  A pair of Mrp1-specific PCR primers were designed, and RT-PCR method was used to study the mRNA's expression pattern in rat molar root and other organs. Gene positioning and other protein sequence prediction were carried out by chromosome analysis and other bioinformatics analysis.
  
  
  RESULTS
  Mrp1 was expressed not only in the molar but also in the developing pancreas, liver, lung and kidney tissues. Mrp1 was located in the 18q12.3 chromosome of the rats and the Mrp1 amino acids sequence had about 37% homology with a known protein Uroplakin IIIb (p35) which was an urothelial differentiation membrane molecular marker. A trans-membrane structure, 5 PKC phosphorylation sites and 4 CKII phosphorylation sites in Mrp1 were found.
  
  
  CONCLUSIONS
  Mrp1 has a broad expression in different developing organs, and it may have a important function in the rat tooth root development.
authors.affiliation: Department of Oral Histopathology, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Xing
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Shi-bin
authors.initials: SB
authors.lastname: Yu
authors.forename: Kun
authors.initials: K
authors.lastname: Xuan
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Ling-ying
authors.initials: LY
authors.lastname: Wen
mesh_terms: D000818:Animals; D019295:Computational Biology; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D008963:Molar / Q000254:growth & development*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014092:Tooth Root
chemical_list: D021382:Protein Sorting Signals; D011506:Proteins