title: FAT/CD36 expression is not ablated in spontaneously hypertensive rats.
abstract: There is doubt whether spontaneously hypertensive rats (SHR; North American strain) are null for fatty acid translocase (FAT/CD36). Therefore, we examined whether FAT/CD36 is expressed in heart, muscle, liver and adipose tissue in SHR. Insulin resistance was present in SHR skeletal muscle. We confirmed that SHR expressed aberrant FAT mRNAs in key metabolic tissues; namely, the major 2.9 kb transcript was not expressed, but 3.8 and 5.4 kb transcripts were present. Despite this, FAT/CD36 protein was expressed in all tissues, although there were tissue-specific reductions in FAT/CD36 protein expression and plasmalemmal content, ranging from 26-85%. Fatty acid transport was reduced in adipose tissue (-50%) and was increased in liver (+47%). Normal rates of fatty acid transport occurred in heart and muscle, possibly due to compensatory upregulation of plasmalemmal fatty acid binding protein (FABPpm) in red (+123%) and white muscle (+110%). In conclusion, SHRs (North American strain) are not a natural FAT/CD36 null model, the North American strain of SHR express FAT/CD36, albeit at reduced levels.
authors.affiliation: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada. abonen@uoguelph.ca
authors.forename: Arend
authors.initials: A
authors.lastname: Bonen
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Han
authors.forename: Narendra N
authors.initials: NN
authors.lastname: Tandon
authors.forename: Jan F C
authors.initials: JF
authors.lastname: Glatz
authors.forename: James
authors.initials: J
authors.lastname: Lally
authors.forename: Laelie A
authors.initials: LA
authors.lastname: Snook
authors.forename: Joost J F P
authors.initials: JJ
authors.lastname: Luiken
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D018955:CD36 Antigens / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014018:Tissue Distribution
chemical_list: D018955:CD36 Antigens; C008132:Cd36 protein, rat; D005227:Fatty Acids; D012333:RNA, Messenger; D019308:Palmitic Acid; D005947:Glucose