title: Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip.
abstract: The present paper described a rapid and ultrasensitive detection method using a microfluidic chip for analyzing 7-aminoclonazepam (7-ACZP) residues in human urine. A microfluidic chip-based immunoassay with laser-induced fluorescence (LIF) detection based on the water-soluble denatured bovine serum albumin (dBSA)-coated CdTe quantum dots (QDs) was prepared for the ultrasensitive detection of 7-ACZP. The whole procedure including the chip and the control software was designed and constructed in our own laboratory. The detection of 7-ACZP could be completed within 5 min. The results demonstrated that under the optima conditions, 7-ACZP residues could be detected with a precision of 5% relative standard deviation (RSD), and the linear range and the limit of detection (LOD) for 7-ACZP were 1.1-60.1 and 0.021 ngmL(-1), respectively. This method was compared with ELISA and showed a good correlation. This microfluidic chip with LIF detection was applied to the determination of 7-ACZP residues in positive human urine samples, and the results were confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC/MS/MS). This ultrasensitive detection technique was proved to be practical for clinical use.
authors.affiliation: School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Chifang
authors.initials: C
authors.lastname: Peng
authors.forename: Zhengyue
authors.initials: Z
authors.lastname: Jin
authors.forename: Ruirui
authors.initials: R
authors.lastname: Qiao
authors.forename: Wuyang
authors.initials: W
authors.lastname: Wang
authors.forename: Shuifang
authors.initials: S
authors.lastname: Zhu
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Qinhui
authors.initials: Q
authors.lastname: Jin
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000927:Anticonvulsants / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation; D019187:Cadmium Compounds / Q000737:chemistry*; D002998:Clonazepam / Q000031:analogs & derivatives* / Q000652:urine; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008832:Microchemistry / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013691:Tellurium / Q000737:chemistry*; D016482:Urinalysis / Q000295:instrumentation*
chemical_list: D000927:Anticonvulsants; D019187:Cadmium Compounds; C020860:7-aminoclonazepam; D002998:Clonazepam; D013691:Tellurium; C028337:cadmium telluride