title: cDNA sequencing and expression of Nramp1 (Slc11a1) in dogs phenotypically resistant or susceptible to visceral leishmaniasis.
abstract: Nramp1 (Slc11a1) is linked to resistance to Leishmania in mice, but its role in canine leishmaniasis is not clear. In this study we sequenced the Nramp1 cDNA from dogs whose macrophages allowed or restricted intracellular growth of Leishmania chagasi. Peripheral blood monocyte-derived macrophages were isolated from 29 dogs, cultured and inoculated with L. chagasi. This approach resulted in the identification of dogs whose macrophages were resistant or susceptible to L. chagasi. Nramp1 cDNA sequences of these dogs were identical. mRNA levels of Nramp1, IFNgamma, IL-4 and the subunit p35 of IL-12 were assessed in the spleen of naturally infected symptomatic and asymptomatic dogs in comparison to uninfected controls. Although not statistically significant, asymptomatic dogs had a tendency for higher levels of Nramp1 mRNA (p = 0.11). Expression of Nramp1 was then compared between phenotypically resistant and susceptible dogs, without any significant difference between these groups.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Regina
authors.initials: R
authors.lastname: Bueno
authors.forename: Alcina V
authors.initials: AV
authors.lastname: Carvalho Neta
authors.forename: Mariana N
authors.initials: MN
authors.lastname: Xavier
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Oliveira
authors.forename: Soraia A
authors.initials: SA
authors.lastname: Diniz
authors.forename: Maria N
authors.initials: MN
authors.lastname: Melo
authors.forename: Renato L
authors.initials: RL
authors.lastname: Santos
mesh_terms: D000818:Animals; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005786:Gene Expression Regulation / Q000276:immunology; D020022:Genetic Predisposition to Disease; D007898:Leishmaniasis, Visceral / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008264:Macrophages / Q000469:parasitology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D018076:DNA, Complementary; D000099173:Natural Resistance-associated Macrophage Protein 1