Molecular cloning and characterization of the common marmoset huntingtin gene.
We report here for the first time the isolation and identification of the common marmoset (Callithrix jacchus) huntingtin (Htt) gene, whose ortholog in humans is known to be related to Huntington's disease (HD). A 9396 nucleotide complementary DNA (cDNA) carrying the putative full-length open reading frame of the marmoset Htt gene was identified, and highly conserved nucleotide and amino acid sequences among primates were observed. Based on this data and using tools evaluated for the detection of the marmoset Htt gene, we have demonstrated gene silencing against the expression of endogenous Htt gene in immortalized common marmoset mononuclear cells by means of RNA interference (RNAi). Taken together, the data presented here may assist us in realizing a non-human primate HD model with the common marmoset.
Hohjoh
Hirohiko
H
Department of Molecular Genetics, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan. hohjohh@ncnp.go.jp
Akari
Hirofumi
H
Fujiwara
Yuko
Y
Tamura
Yoshiko
Y
Hirai
Hirohisa
H
Wada
Keiji
K
D000595:Amino Acid Sequence; D000818:Animals; D002144:Callithrix / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D009419:Nerve Tissue Proteins; D010455:Peptides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins