[Determination of lobetyolin in root of Codonopsis tangshen from various cultivation areas by high-performance liquid chromatography].
OBJECTIVE
To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas.


METHODS
A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and UV detection was at 268 nm.


RESULTS
Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1).


CONCLUSIONS
The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.
Song
Dan
D
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese medicine, Shanghai 201203, China.
Cheng
Xue-mei
XM
Li
Long-yun
LY
Zhong
Guo-yue
GY
Wang
Zheng-tao
ZT
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029745:Codonopsis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D053279:Polyynes / Q000032:analysis*; D011786:Quality Control
D004365:Drugs, Chinese Herbal; C521561:lobetyolin; D053279:Polyynes