Binding assays for brassinosteroid receptors.
BRI1, a leucine-rich repeat (CRR)-receptor kinase protein, belongs to the plant hormone brassinosteroid (BR) receptor complex in Arabidopsis thaliana (Arabidopsis). The experiments described in this chapter to carry out BR-binding assays in Arabidopsis plants are largely based on previously published procedures by Wang et al. (1) and Caño-Delgado et al. (2), which demonstrated that BRI1 and BRI1-like proteins BRL1 and BRL3, are components of the BR-receptor complexes in vivo. Furthermore, binding assays using a photo-affinity labelled castasterone have demonstrated that BRs are perceived directly by a subdomain of the extracellular domain of BRI1 defined by the 94 amino acids of the ID-LRR22 (3).
Caño-Delgado
Ana
A
Departamento de Genética Molecular, Consorcio CSIC-IRTA, Barcelona, Spain.
Wang
Zhi-Yong
ZY
D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D049452:Green Fluorescent Proteins; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods*; D013256:Steroids / Q000737:chemistry* / Q000378:metabolism*
D029681:Arabidopsis Proteins; D013256:Steroids; D049452:Green Fluorescent Proteins; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis