Angiotensin II induces superoxide generation via NAD(P)H oxidase activation in isolated rat pancreatic islets.
Angiotensin II (Ang II) controls blood pressure, electrolyte balance, cell growth and vascular remodeling. Ang II activates NAD(P)H oxidase in several tissues with important function in the control of insulin secretion. Considering the concomitant occurrence of hypertension, insulin resistance and pancreatic B cell secretion impairment in the development of type II diabetes the aim of the present study was to evaluate the effect of ANG II on NAD(P)H oxidase activation in isolated pancreatic islets. We found that ANGII-induced superoxide generation via NAD(P)H oxidase activation and increased protein and mRNA levels of NAD(P)H oxidase subunits (p47(PHOX) and gp91(PHOX)).
Hirata
A E
AE
Department of Physiology, Federal University of Sao Paulo-UNIFESP, São Paulo, Brazil. emiko.hirata@unifesp.br
Morgan
D
D
Oliveira-Emilio
H R
HR
Rocha
M S
MS
Carvalho
C R O
CR
Curi
R
R
Carpinelli
A R
AR
D000804:Angiotensin II / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D004789:Enzyme Activation; D005260:Female; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism*; D051381:Rats; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D044140:Receptor, Angiotensin, Type 1; D013481:Superoxides; D000804:Angiotensin II; D019255:NADPH Oxidases