title: [Study of rapid method on enterotoxigenic Escherichia coli detected by real-time fluoresence quantitative PCR].
abstract: OBJECTIVE
  To develop a real-time PCR for detecting enterotoxigenic Escherichia coli (ETEC) based on TaqMan technology.
  
  
  METHODS
  Primers and probes were designed in the coding region of heat-stable enterotoxin, heat-labile enterotoxin of ETEC. ETEC were detected by real-time fluoresence quantitative PCR, making use of the exterior standard curve which was described by several different concentration. The speciality, sensitivity, accuracy, repetition, and stability of real-time fluoresence quantitative PCR system were evaluated.
  
  
  RESULTS
  Primers and TaqMan probes were suited to the real-time fluoresence quantitative PCR. The assay showed that the method could be rapid, special, sensitive and stabile. The real-time PCR system could detect ETEC between 10(0)-10(7) DNA copies/reaction. The assay should be finished in two hours.
  
  
  CONCLUSIONS
  It was suggested that real-time fluoresence quantitative PCR based on TaqMan probe could be a rapid, sensitive and special method. It is significant that the excellent method could control diarrhoea caused by ETEC.
authors.affiliation: Lab Medicine Department, Chengdu Medical College, Chengdu 610083, China. daijuan_2004_2007@126.com
authors.forename: Juan
authors.initials: J
authors.lastname: Dai
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Lixing
authors.initials: L
authors.lastname: Yuan
mesh_terms: D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes