An emulsion system based on a chip polymerase chain reaction.
In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on "on-chip" PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other related PCR components were dispersed in different droplets of the emulsion system, and then, due to the thermal instability of emulsion droplets, they would be released onto the surface of the slide after preheating in the first PCR step. To test the above method, we used plasma DNAs from pregnant women who was carrying a male fetus for gender identification. Four different Y chromosome DNA fragments were selected. Results showed that different DNA fragments could be simultaneously amplified with satisfactory results. It is suggested that a simple, convenient and inexpensive on-chip PCR method has been developed.
Ge
Qinyu
Q
Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, PR China. geqinyu@seu.edu.cn
Yu
Pinfei
P
Bai
Yunfei
Y
Lu
Zuhong
Z
D004247:DNA / Q000097:blood; D004655:Emulsions / Q000737:chemistry*; D005260:Female; D005333:Fetus; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D015203:Reproducibility of Results; D012732:Sex Determination Analysis; D013696:Temperature
D004655:Emulsions; D004247:DNA