Microencapsulation of Lactobacillus plantarum spp in an alginate matrix coated with whey proteins.
Whey proteins were used as a coating material to improve encapsulation of Lactobacillus plantarum strains in calcium alginate beads. L. plantarum 299v, L. plantarum 800 and L. plantarum CIP A159 were used in this study. Inactivation experiments were carried out in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Cross-sections of freeze-dried beads revealed the random distribution of bacteria throughout the alginate network. From an initial count of 10.04+/-0.01 log(10) CFU g(-1) for L. plantarum 299v, 10.12+/-0.04 for L. plantarum CIP A159 and 10.03+/-0.01 for L. plantarum 800, bacteria in coated beads and incubated in SGF (37 degrees C, 60 min) showed a better survival for L. plantarum 299v, L. plantarum CIP A159 and L. plantarum 800 (respectively 7.76+/-0.12, 6.67+/-0.08 and 5.81+/-0.25 log(10) CFU g(-1)) when compared to uncoated beads (2.19+/-0.09, 1.89+/-0.09 and 1.65+/-0.10 log(10) CFU g(-1)) (p<0.05). Only bacteria in the coated beads survived in the SIF medium (37 degrees C, 180 min) after SGF treatment. This preliminary work showed that whey proteins are a convenient, cheap and efficient material for coating alginate beads loaded with bacteria.
Gbassi
Gildas Komenan
GK
IPHC, Laboratoire de Chimie Analytique et Sciences de l'Aliment (UMR-7178) Faculté de Pharmacie, ULP-CNRS, Illkirch-Graffenstaden, France. gildas.gbassi-komenan@ulp.u-strasbg.fr
Vandamme
Thierry
T
Ennahar
Saïd
S
Marchioni
Eric
E
D000464:Alginates / Q000737:chemistry*; D015169:Colony Count, Microbial; D004339:Drug Compounding / Q000379:methods*; D005524:Food Technology; D005744:Gastric Acid / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000254:growth & development*; D050296:Microbial Viability; D008894:Milk Proteins / Q000737:chemistry*; D019936:Probiotics; D000067816:Whey Proteins
D000464:Alginates; D006603:Hexuronic Acids; D008894:Milk Proteins; D000067816:Whey Proteins; D020723:Glucuronic Acid