The role of peroxiredoxin III in the ototoxic drug-induced mitochondrial apoptosis of cochlear hair cells.
CONCLUSIONS
Peroxiredoxin (Prx) III plays a protective role in cisplatin- and gentamicin-induced apoptosis via a mitochondria-dependent pathway in cochlear hair cells.


OBJECTIVE
The aim of the present study was to investigate the effects of the depletion of endogenous Prx III on oxidative damage to mitochondrial components and on apoptotic events with the use of RNA interference.


METHODS
. Depletion of Prx III was done by RNA interference in cochlear hair cells (UB/OC-1), and cells were exposed to ototoxic drugs (cisplatin or gentamicin). We then compared the apoptotic signaling between Prx III-depleted and control cells.


RESULTS
The depletion of Prx III resulted in increased intracellular ROS levels accompanied by enhanced apoptosis by ototoxic drugs. The Prx III-depleted cells showed mitochondrial membrane potential collapse, cytochrome c release, and caspase activation.
Choi
Ho Seok
HS
Department of Otorhinolaryngology-Head & Neck Surgery, Inha University College of Medicine, Incheon, South Korea.
Park
Kwang Joo
KJ
Hwang
Sung Chul
SC
Park
Hun Yi
HY
Kim
Young Sun
YS
Park
Keehyun
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques; D002945:Cisplatin / Q000494:pharmacology; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D005839:Gentamicins / Q000494:pharmacology; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D061105:Peroxiredoxin III; D054464:Peroxiredoxins / Q000502:physiology*; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D005839:Gentamicins; D017382:Reactive Oxygen Species; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III; D054464:Peroxiredoxins; D002945:Cisplatin