title: Crystallization and preliminary crystallographic analysis of laminarinase from Rhodothermus marinus: a case of pseudomerohedral twinning.
abstract: Thermophilic endo-1,3(4)-beta glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method. The needle-like crystals belong to space group P2(1) and contain two protein molecules in the asymmetric unit with a solvent content of 51.75 %. Diffraction data were collected to a resolution of 1.95A and resulted in a dataset with an overall R(merge) of 10.4% and a completeness of 97.8%. Analysis of the structure factors revealed pseudomerohedral twinning of the crystals with a twin fraction of approximately 42%.
authors.affiliation: Petersburg Nuclear Physics Institute, Gatchina, St. Petersbug 188300, Rusia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Golubev
authors.forename: Adriana L
authors.initials: AL
authors.lastname: Rojas
authors.forename: Alessandro S
authors.initials: AS
authors.lastname: Nascimento
authors.forename: Lucas
authors.initials: L
authors.lastname: Bleicher
authors.forename: Anna A
authors.initials: AA
authors.lastname: Kulminskaya
authors.forename: Elena V
authors.initials: EV
authors.lastname: Eneyskaya
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D044644:Endo-1,3(4)-beta-Glucanase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D009154:Mutation; D045245:Rhodothermus / Q000201:enzymology*
chemical_list: D044644:Endo-1,3(4)-beta-Glucanase