title: 1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor.
abstract: The possibility of using the pyrene metabolite 1-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs - phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days of exposure the concentrations of parent PAHs and 1-hydroxypyrene were identified using three different analytical methods, high-performance liquid chromatography with fluorescence detection (HPLC/F), synchronous fluorescence spectroscopy (SFS) and gas chromatography with mass spectrometric detection (GC/MS). The SFS measurements of 1-hydroxypyrene were validated by the more sensitive method HPLC/F. The positive correlation between total PAHs and 1-hydroxypyrene concentrations in the polychaete tissues observed in experiments, suggests the feasibility of 1-hydroxypyrene as a suitable biomarker for total PAH exposure assessment. Furthermore, the possibility of employment of the simple and rapid SFS method instead of HPLC/F for biomarker analysis has been confirmed by the positive and significant correlation between results achieved by these two analytical methods.
authors.affiliation: Department of Science, Systems and Models, Roskilde University, Universitetsvej 1, DK-4000 Roskilde, Denmark. zhanna@ruc.dk
authors.forename: Zhanna M
authors.initials: ZM
authors.lastname: Tairova
authors.forename: Anders M B
authors.initials: AM
authors.lastname: Giessing
authors.forename: Rikke
authors.initials: R
authors.lastname: Hansen
authors.forename: Ole
authors.initials: O
authors.lastname: Andersen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D019015:Geologic Sediments / Q000737:chemistry; D008386:Marine Biology; D011077:Polychaeta / Q000737:chemistry / Q000187:drug effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011721:Pyrenes / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D014874:Water Pollutants, Chemical; C033146:1-hydroxypyrene