HOXA10 suppresses p/CAF promoter activity via three consecutive TTAT units in human endometrial stromal cells.
Implantation is the first maternal-embryo crosstalk that only occurs during a finite period called the 'implantation window'. HOXA10, a homeobox transcription factor, plays an important regulatory role during this period. However, the target genes of HOXA10 involved in implantation and decidualization have not been identified. Using a chromatin immunoprecipitation screen, we identified the p300/CBP-associated factor (p/CAF) as a direct HOXA10 target gene in vivo. Adenovirus-mediated overexpression and siRNA-specific knockdown of HOXA10 altered p/CAF promoter activity via interaction with the three consecutive TTAT element units in human endometrial cells. These results indicate that p/CAF is a novel HOXA10 target gene, and HOXA10 promotes human endometrial development, at least in part, through the regulation of p/CAF gene.
Sun
Haixiang
H
College of Life Sciences, Nanjing Normal University, Nanjing, PR China.
Chen
Linjun
L
Yan
Guijun
G
Wang
Ruina
R
Diao
Zhenyu
Z
Hu
Yali
Y
Li
Chaojun
C
D001483:Base Sequence; D015536:Down-Regulation; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011401:Promoter Regions, Genetic; D017154:Stromal Cells / Q000378:metabolism; D050880:p300-CBP Transcription Factors / Q000235:genetics*; D000099224:p300-CBP-Associated Factor
D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C069634:HOXA10 protein, human