title: Biodegradation of 1,4-dioxane and transformation of related cyclic compounds by a newly isolated Mycobacterium sp. PH-06.
abstract: A new bacterial strain PH-06 was isolated using enrichment culture technique from river sediment contaminated with 1,4-dioxane, and identified as belonging to the genus Mycobacterium based on 16S rRNA sequencing (Accession No. EU239889). The isolated strain effectively utilized 1,4-dioxane as a sole carbon and energy source and was able to degrade 900 mg/l 1,4-dioxane in minimal salts medium within 15 days. The key degradation products identified were 1,4-dioxane-2-ol and ethylene glycol, produced by monooxygenation. Degradation of 1,4-dioxane and concomitant formation of metabolites were demonstrated by GC/MS analysis using deuterium labeled 1,4-dioxane (1,4-dioxane-d8). In addition to 1,4-dioxane, this bacterium could also transform structural analogues such as 1,3-dioxane, cyclohexane and tetrahydrofuran when pre-grown with 1,4-dioxane as the sole growth substrate. Our results suggest that PH-06 can maintain sustained growth on 1,4-dioxane without any other carbon sources.
authors.affiliation: School of Environmental Science and Engineering, POSTECH, Pohang, South Korea.
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kim
authors.forename: Jong-Rok
authors.initials: JR
authors.lastname: Jeon
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Murugesan
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kim
authors.forename: Yoon-Seok
authors.initials: YS
authors.lastname: Chang
mesh_terms: D004146:Dioxanes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004146:Dioxanes; D012336:RNA, Ribosomal, 16S; C025223:1,4-dioxane