Comparison of luminescent immunoassays using biotinylated proteins of aequorin, alkaline phosphatase and horseradish peroxidase as reporters.
We compare aequorin, alkaline phosphatase and horseradish peroxidase as reporters for luminescent immunoassays using alpha-fetoprotein (AFP) as a model analyte. Biotinylated aequorin was prepared from mutated aequorin containing a reactive cysteine residue by chemical conjugation. The measurable range of AFP using this biotinylated aequorin was 0.02-200 ng/ml, with a lower background level than the other biotinylated enzymes tested.
Inouye
Satoshi
S
Yokohama Research Center, Chisso Corporation, Yokohama, Japan. sinouye@chisso.co.jp
Sato
Jun-ichi
J
D000331:Aequorin / Q000032:analysis / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D019921:Biotinylation; D006735:Horseradish Peroxidase / Q000032:analysis / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D000509:alpha-Fetoproteins; D000331:Aequorin; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase