[Expression of EBI3 and p28 mRNA in the brain and spinal cord of chronic model of experimental autoimmune encephalomyelitis in C57BL/6J mice].
OBJECTIVE
To investigate the expression of EBI3 and p28 mRNA (the 2 subunits of IL-27) in the brain and spinal cord of the model of experimental autoimmune encephalomyelitis (EAE), and to explore their effect on EAE.


METHODS
Seventy-two adult female SPF C57BL/6J mice (inbred strain) were randomly divided into a control group, an adjuvant group, and an EAE group. RT-PCR was performed to detect the expression of EBI3 mRNA and p28 mRNA in the brain and spinal cord.


RESULTS
The expression of EBI3 mRNA and p28 mRNA was up-regulated at onset in the EAE group, which increased quickly during peak phase and maintained at a high level in the chronic phase. There was significant difference in the expression of EBI3 and p28 mRNA between the EAE group and the control/adjuvant group (P<0.01). Additionally, there was no remarkable difference in the expression of EBI3 and p28 mRNA in the brain and spinal cord between the control group and the adjuvant group (P>0.05).


CONCLUSIONS
IL-27 may play a role of promoting the morbility of EAE in the early stage, and sustain the inflammatory response in endgame.
Yan
Jin-jin
JJ
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
Wang
Rui
R
Wang
Yu-zhong
YZ
Zhou
Wen-bin
WB
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002908:Chronic Disease; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*
C499601:Ebi3 protein, mouse; C486767:Il27 protein, mouse; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; D021122:Protein Subunits; D012333:RNA, Messenger; D018121:Receptors, Cytokine