Simultaneous determination of three anticonvulsants using hydrophilic interaction LC-MS.
A specific and automated method was developed to quantify the anticonvulsants gabapentin, pregabalin and vigabatrin simultaneously in human serum. Samples were prepared with a protein precipitation. The hydrophilic interaction chromatography (HILIC) with a mobile phase gradient was used to divide off ions of the matrix and for separation of the analytes. Four different HILIC-columns and two different column temperatures were tested. The Tosoh-Amid column gave the best results: single small peaks. The anticonvulsants were detected in the multiple reaction monitoring mode (MRM) with ESI-MS-MS. Using a volume of 100 microL biological sample the lowest point of the standard curve, i.e. the lower LOQs were 312 ng/mL. The described HILIC-MS-MS method is suitable for therapeutic drug monitoring and for clinical and pharmcokinetical investigations of the anticonvulsives.
Oertel
Reinhard
R
Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany. reinhard.oertel@tu-dresden.de
Arenz
Norman
N
Pietsch
Jörg
J
Kirch
Wilhelm
W
D000588:Amines / Q000032:analysis* / Q000737:chemistry; D000927:Anticonvulsants / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis* / Q000737:chemistry; D000077206:Gabapentin; D000069583:Pregabalin; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D020888:Vigabatrin / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry
D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D012996:Solutions; D014867:Water; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D020888:Vigabatrin