title: Molecular diagnosis of acute retinal necrosis secondary to cytomegalovirus in vitreous aspirate.
abstract: We report a case of a 45-year old Filipino post-kidney transplant patient maintained on steroids, who presented with floaters in her left eye. Vitreous aspirate was analyzed using polymerase chain reaction (PCR) for human cytomegalovirus (HCMV) and herpes simplex virus (HSV). A distinct band (435 bp) was found that confirmed the presence of HCMV. Since a rapid and accurate diagnosis is crucial for prompt administration of antiviral therapy, PCR-based analysis of vitreous aspirate provides a valuable tool in the diagnosis of patients with retinitis caused by herpes viruses.
authors.affiliation: Research and Biotechnology Division, St Luke's Medical Center, Quezon City, 1102 Philippines. mobaclig@stluke.com.ph
authors.forename: Michael O
authors.initials: MO
authors.lastname: Baclig
authors.forename: Ma Luisa G
authors.initials: ML
authors.lastname: Daroy
authors.forename: Jessica Marie R
authors.initials: JM
authors.lastname: Abano
mesh_terms: D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications*; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000209:etiology* / Q000821:virology; D005260:Female; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015882:Retinal Necrosis Syndrome, Acute / Q000175:diagnosis / Q000209:etiology* / Q000821:virology; D014792:Visual Acuity; D014822:Vitreous Body / Q000821:virology