Significant correlation between maternal body mass index at delivery and in the second trimester, and second trimester circulating total cell-free DNA levels.
OBJECTIVE
The use of cell-free fetal deoxyribonucleic acid (DNA) requires the knowledge of variables that may influence the levels of cell-free DNA, such as maternal body mass index (BMI).


METHODS
In this study, using 406 maternal blood samples from the second trimester of pregnancy, cell-free fetal DNA specific for the SRY and DYS14 loci and glyceraldehyde-3-phosphate dehydrogenase sequence were quantified by real-time polymerase chain reaction.


RESULTS
No significant correlation was seen between the levels of cell-free fetal DNA and maternal BMI, whereas total cell-free DNA was significantly associated with maternal BMI at 20 to 21 weeks of gestation (P = .034) and at the end of pregnancy (R2 regression: 0.016, P = .014).


CONCLUSIONS
Quantitative levels of cell-free fetal DNA are not affected by maternal BMI, whereas total DNA levels in the second trimester significantly correlate with maternal BMI at the moment of blood drawing and at delivery.
Lapaire
Olav
O
Department of Obstetrics and Gynecology, University of Basel, Basel, Switzerland.
Volgmann
Thorsten
T
Grill
Simon
S
Hösli
Irène
I
Zanetti-Daellenbach
Rosanna
R
Zhong
Xiao Yan
XY
Holzgreve
Wolfgang
W
D000328:Adult; D015415:Biomarkers / Q000097:blood; D001724:Birth Weight; D015992:Body Mass Index; D004247:DNA / Q000097:blood*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005858:Germany; D005865:Gestational Age; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008431:Maternal-Fetal Exchange; D036801:Parturition; D010920:Placenta / Q000098:blood supply; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000097:blood; D011446:Prospective Studies; D015203:Reproducibility of Results; D012732:Sex Determination Analysis; D051876:Sex-Determining Region Y Protein / Q000235:genetics; D013557:Switzerland; D055815:Young Adult
D015415:Biomarkers; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D004247:DNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases