[Action of an active compound RG2-1 from radix gentianae on antirespiratory syncytial virus in vitro].
OBJECTIVE
To observe an action of an active compound RG2-1 from Radix Gentianae on the antirespiratory syncytial virus(RSV) in vitro.


METHODS
RG2-1 from Radix Gentianae was extracted by hot water extraction and ethanol precipitation. The cytotoxic and antiviral activity of RG2-1 were tested by MTT and the CPE inhibition assay, while ribavirin was used as a positive control.


RESULTS
The median toxic concentrations (TC50) of RG2-1 were 11.07 mg/ml. The direct inactivating effect, inhibition of endogenous multiplication and preventive effect of RG2-1 against RSV were observed. And the median effective concentrations (EC50) of three effects were 0.43, 0.42 and 0.48 mg/ml, respectively. The treatment indexes (TI) were 25.7, 26.36 and 23.06, respectively.


CONCLUSIONS
RG2-1 could have significant anti-RSV effect in vitro.
Li
Xin
X
School of Public Health, Harbin Medical University, Harbin 150086, China. lixin9191@163.com
Yang
Xiujing
X
Tian
Wenjing
W
Li
Hongyuan
H
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D030012:Gentiana / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal