In vivo evaluation of leukemic stem cells through the xenotransplantation model.
The xenotransplantation model has been instrumental for the identification and characterization of human leukemic stem cells. This unit describes our current method for the engraftment of human leukemic patients' samples in the xenotransplanted mouse model. We concentrate uniquely on the model of acute myeloid leukemia, as it was the first type of leukemia for which the xenotransplantation model was developed. Nevertheless, the Basic Protocol could be applied to other sorts of blood disorders.
Bonnet
Dominique
D
Cancer Research UK, London Research Institute, Haematopoietic Stem Cell Laboratory, London, United Kingdom.
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology; D008722:Methods; D051379:Mice; D009368:Neoplasm Transplantation / Q000379:methods*; D014411:Neoplastic Stem Cells / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000379:methods*