title: Induction of Epstein-Barr virus (EBV) lytic cycle in vitro causes oxidative stress in lymphoblastoid B cell lines.
abstract: Here, we investigated the effect of induction of the Epstein-Barr virus (EBV) viral lytic cycle on the oxidant/antioxidant balance in three lymphoblastoid cell lines: B95-8, Raji, and LCL C1. The induction of the EBV lytic cycle was done by a non-stressing dose of 12-0-tetradecanoylphorbol-13-acetate (8 nM). Oxidative stress was assessed by measuring malondialdehyde as a parameter of lipid peroxidation, the levels of glutathione, and the activities of three antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase). After 48 h (peak of lytic cycle), a significant decrease in superoxide dismutase activity was observed in B95-8, Raji, and LCL C1 cells (P < 0.05). In addition, in B95-8 cells also a significant decrease of catalase activity was detected (P < 0.05). The glutathione peroxidase activity and the glutathione level were not significantly modified by the induction in any of the cell lines. We found a significant rise in malondialdehyde levels in B95-8, Raji, and LCL C1 cells after the induction of the lytic cycle compared to controls (P < 0.05). In conclusion, induction of EBV lytic cycle in lymphoblastoid cells causes increased oxidative stress in the host cells within 48 h, a process that could be involved in malignant transformations.
authors.affiliation: Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, Sfax, Tunisia. bochragargouri@yahoo.fr
authors.forename: Bochra
authors.initials: B
authors.lastname: Gargouri
authors.forename: Jos
authors.initials: J
authors.lastname: Van Pelt
authors.forename: Abd El Fatteh
authors.initials: Ael F
authors.lastname: El Feki
authors.forename: Hammadi
authors.initials: H
authors.lastname: Attia
authors.forename: Saloua
authors.initials: S
authors.lastname: Lassoued
mesh_terms: D000975:Antioxidants / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral; D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D015448:Leukemia, B-Cell / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D016877:Oxidants / Q000032:analysis; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016877:Oxidants; D010088:Oxidoreductases