[Characteristics of the immune response to the lethal toxin of Bacillus anthracis].
We and other authors have recently shown that the pattern of the immune response to components of anthrax, the Bacillus anthracis lethal toxin, is complex. In addition to neutralizing antibodies, the antitoxin antibody pool contains antibodies enhancing the toxin lethal action. We mapped the epitopes in the protective antigen that are responsible for the induction of both antibody types. In this study, we obtained new data on the cytotoxicity of the B. anthracis lethal toxin toward the J774 A.1 cell line in the presence of monoclonal antibodies to various domains of the protective antigen and the lethal factor. The role of the Fc fragment of immunoglobulins in enhancing the lethal toxin action was shown. These results may serve as a basis for the development of a new generation vaccine for anthrax.
Belova
E V
EV
Kolesnikov
A V
AV
Zakharova
M Iu
MIu
Dubileĭ
S A
SA
Diatlov
I A
IA
Shemiakin
I G
IG
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000276:immunology*; D001408:Bacillus anthracis / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D002460:Cell Line; D018604:Epitope Mapping; D005098:Exotoxins / Q000276:immunology*; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D051379:Mice
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D005098:Exotoxins; D007141:Immunoglobulin Fc Fragments; C030325:anthrax toxin