title: Polymerized lipid bilayers on a solid substrate: morphologies and obstruction of lateral diffusion.
abstract: Substrate supported planar lipid bilayers (SPBs) are versatile models of the biological membrane in biophysical studies and biomedical applications. We previously developed a methodology for generating SPBs composed of polymeric and fluid phospholipid bilayers by using a photopolymerizable diacetylene phospholipid (DiynePC). Polymeric bilayers could be generated with micropatterns by conventional photolithography, and the degree of polymerization could be controlled by modulating UV irradiation doses. After removing nonreacted monomers, fluid lipid membranes could be integrated with polymeric bilayers. Herein, we report on a quantitative study of the morphology of polymeric bilayer domains and their obstruction toward lateral diffusion of membrane-associated molecules. Atomic force microscopy (AFM) observations revealed that polymerized DiynePC bilayers were formed as nanometer-sized domains. The ratio of polymeric and fluid bilayers could be modulated quantitatively by changing the UV irradiation dose for photopolymerization. Lateral diffusion coefficients of lipid molecules in fluid bilayers were measured by fluorescence recovery after photobleaching (FRAP) and correlated with the amount of polymeric bilayer domains on the substrate. Controlled domain structures, lipid compositions, and lateral mobility in the model membranes should allow us to fabricate model membranes that mimic complex features of biological membranes with well-defined structures and physicochemical properties.
authors.affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda 563-8577, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okazaki
authors.forename: Takehiko
authors.initials: T
authors.lastname: Inaba
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Tatsu
authors.forename: Ryugo
authors.initials: R
authors.lastname: Tero
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Urisu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Morigaki
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D004058:Diffusion; D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D001704:Biopolymers; D008051:Lipid Bilayers