[Observation of ultrastructure and ECP expression in nasal polyps].
OBJECTIVE
To observe the ultrastructural feature and ECP expression in nasal polyps, and aim to explore its role in the pathogenesis of CRSwNP.


METHODS
5 CRSwNP, 5 CRS and 5 control patients underwent sinus surgery were gathered to detect its ultrastructure and expression of ECP by in situ hybridization and electron microscopy technique.


RESULTS
Under electron microscopy, the eosinophilic cells in CRSwNP group increased, its membrane was intact but fold, the feature of pseudopodium, degranulation and cavitation were all found. The expression level of ECP mRNA was up-regulated.


CONCLUSIONS
The findings suggest that eosinophilic infiltrate and ECP cytologic inflammation reactions may involve in the pathogenesis of CRSwNP.
Xia
Zhongfang
Z
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Kong
Weijia
W
Yue
Jianxin
J
Wang
Yanjun
Y
Wu
Lisha
L
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D047150:Eosinophil Cationic Protein / Q000378:metabolism*; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D009298:Nasal Polyps / Q000378:metabolism* / Q000648:ultrastructure*; D055815:Young Adult
D047150:Eosinophil Cationic Protein