title: Antiproliferative cardenolide glycosides of Elaeodendron alluaudianum from the Madagascar Rainforest.
abstract: Bioassay-guided fractionation of an ethanol extract of a Madagascar collection of Elaeodendron alluaudianum led to the isolation of two new cardenolide glycosides (1 and 2). The (1)H and (13)C NMR spectra of both compounds were fully assigned using a combination of 2D NMR experiments, including (1)H-(1)H COSY, HSQC, HMBC, and ROESY sequences. Both compounds 1 and 2 were tested against the A2780 human ovarian cancer cell line and the U937 human histiocytic lymphoma cell line assays, and showed significant antiproliferative activity with IC(50) values of 0.12 and 0.07 microM against the A2780 human ovarian cancer cell line, and 0.15 and 0.08 microM against the U937 human histiocytic lymphoma cell line, respectively.
authors.affiliation: Department of Chemistry, Virginia Polytechnic Institute and State University, 3111 Hahn Hall, Blacksburg, M/C 0212, VA 24061-0212, USA.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Hou
authors.forename: Shugeng
authors.initials: S
authors.lastname: Cao
authors.forename: Peggy
authors.initials: P
authors.lastname: Brodie
authors.forename: Martin
authors.initials: M
authors.lastname: Callmander
authors.forename: Fidisoa
authors.initials: F
authors.lastname: Ratovoson
authors.forename: Richard
authors.initials: R
authors.lastname: Randrianaivo
authors.forename: Etienne
authors.initials: E
authors.lastname: Rakotobe
authors.forename: Vincent E
authors.initials: VE
authors.lastname: Rasamison
authors.forename: Stephan
authors.initials: S
authors.lastname: Rakotonandrasana
authors.forename: Karen
authors.initials: K
authors.lastname: TenDyke
authors.forename: Edward M
authors.initials: EM
authors.lastname: Suh
authors.forename: David G I
authors.initials: DG
authors.lastname: Kingston
mesh_terms: D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029753:Celastraceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008270:Madagascar; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002298:Cardenolides; D006027:Glycosides