Counting and determining the viability of cultured cells.
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.
Ricardo
Richard
R
Molecular Pathology Laboratory Network, Inc., USA.
Phelan
Katy
K
D000818:Animals; D002452:Cell Count / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D006801:Humans; D014343:Trypan Blue / Q000737:chemistry
D014343:Trypan Blue