title: A survey of Clostridium spiroforme antimicrobial susceptibility in rabbit breeding.
abstract: Rabbit meat breeding may be heavily affected by enterotoxaemia due to Clostridium spiroforme. Data on its antimicrobial susceptibility are insufficient, presumably because of difficulties in cultivating and identifying the pathogen. Our aim is therefore to provide this information to veterinary practitioners by focusing on a panel of therapeutics used in intensive rabbit units. Lincomycin was also checked in order to investigate the origin of resistance to macrolides. Minimal inhibitory concentrations (MICs) were determined with the agar dilution method according to the CLSI M11-A7 protocol (2007). MIC(50) and MIC(90) were, respectively, 64 and 64microg/ml for tiamulin, 32 and 32microg/ml for norfloxacin, 0.063 and 0.125microg/ml for amoxicillin, and 8 and 16microg/ml for doxycycline. MIC(50) and MIC(90) were 256microg/ml for sulphadimethoxine, spiramycin and lincomycin. Our results have shown that intrinsic or acquired antimicrobial resistances are diffuse in the C. spiroforme population and suggest focusing on prevention rather than on treatment of clostridial overgrowth, by reducing risk factors and using antimicrobials prudently.
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Venezie, viale Brigata Treviso 13/a - 31100 Treviso, Italy. fagnoletti@izsvenezie.it
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Agnoletti
authors.forename: Tiziana
authors.initials: T
authors.lastname: Ferro
authors.forename: Angela
authors.initials: A
authors.lastname: Guolo
authors.forename: Barbara
authors.initials: B
authors.lastname: Marcon
authors.forename: Monia
authors.initials: M
authors.lastname: Cocchi
authors.forename: Ilenia
authors.initials: I
authors.lastname: Drigo
authors.forename: Elena
authors.initials: E
authors.lastname: Mazzolini
authors.forename: Luca
authors.initials: L
authors.lastname: Bano
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003013:Clostridium / Q000187:drug effects* / Q000254:growth & development; D003015:Clostridium Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D004767:Enterotoxemia / Q000188:drug therapy / Q000382:microbiology*; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D011817:Rabbits / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents