Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo.
Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca(2+) uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca(2+) uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca(2+) (0.02 mM) freshwater stimulated whole body Ca(2+) influx and zebrafish epithelial Ca(2+) channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca(2+) content, Ca(2+) influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca(2+) uptake in zebrafish embryos.
Tseng
Deng-Yu
DY
Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, Taiwan.
Chou
Ming-Yi
MY
Tseng
Yung-Che
YC
Hsiao
Chung-Der
CD
Huang
Chang-Jen
CJ
Kaneko
Toyoji
T
Hwang
Pung-Pung
PP
D000818:Animals; D030801:Animals, Genetically Modified; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000096:biosynthesis / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization; D008845:Microinjections; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D015347:RNA Probes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels; D014018:Tissue Distribution; D015027:Zebrafish / Q000502:physiology*; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics
D015220:Calcium Channels; D006023:Glycoproteins; D020319:Oligodeoxyribonucleotides, Antisense; D015347:RNA Probes; D050916:TRPV Cation Channels; D029961:Zebrafish Proteins; C505134:trpv6 protein, zebrafish; D012313:RNA; C051795:teleocalcin; D002118:Calcium