Inhibitory effect of blocking TGF-beta/Smad signal on injury-induced fibrosis of corneal endothelium.
OBJECTIVE
To understand the role of TGF-beta related signals in the repair of a corneal endothelium defect and also to evaluate the therapeutic effect of Smad7 gene transfer on injury induced fibrosis of the corneal endothelium in rats.


METHODS
(1) Japanese albino rabbits (n=108) were used. Blocks of central cornea (4 x 4 mm) were prepared. After partially scraping the endothelium to produce a defect, the blocks were organ cultured for 24 h in the presence of either exogenous growth factors, transforming growth factor beta (TGF-beta)-neutralizing antibody, or inhibitors of each TGF-beta related signal. Endothelium repair was assayed under light microscopy. (2) Adult Wistar rats (n=62) were then used. Smad7 expressing adenoviral vector (Smad7-Ad) or non-functioning control vector (Cre-Ad) was administered to the anterior chamber of an eye. The cornea was burned with topical 1 N NaOH (10 microl) three days later. After specific intervals, the eye was histologically observed.


RESULTS
(1) The endothelial layer that elongated toward the defect lacked proliferation after 24 h in organ culture. Endogenous TGF-beta was required for endothelium defect repair. Inhibition of p38 and Erk but not c-Jun NH(2)-terminal kinase (JNK) and ALK5 signal (Smad) retarded such cell spreading. (2) Adenoviral Smad7 overexpression suppressed fibrogenic reaction of the endothelium of an alkali-burned cornea as evaluated by immunohistochemistry for phospho-Smad2, collagen I, and alpha-smooth muscle actin, a marker for endothelial-mesenchymal transition (EnMT), and by electron microscopy.


CONCLUSIONS
Inhibition of Smad and JNK signals do not affect corneal endothelium defect repair. Inhibition of Smad suppresses fibrogenic reaction via EnMT of corneal endothelium in vivo.
Sumioka
Takayoshi
T
Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan. sumioka@wakayama-med.ac.jp
Ikeda
Kazuo
K
Okada
Yuka
Y
Yamanaka
Osamu
O
Kitano
Ai
A
Saika
Shizuya
S
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002056:Burns / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology* / Q000648:ultrastructure; D005355:Fibrosis; D005822:Genetic Vectors / Q000235:genetics; D019426:Integrases / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests; D009924:Organ Culture Techniques; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D051785:Smad Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014161:Transduction, Genetic; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
D000906:Antibodies; D016207:Cytokines; D051785:Smad Proteins; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; C045073:Cre recombinase; D019426:Integrases