Enumeration of bacteriophages by double agar overlay plaque assay.
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.
Kropinski
Andrew M
AM
Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
Mazzocco
Amanda
A
Waddell
Thomas E
TE
Lingohr
Erika
E
Johnson
Roger P
RP
D000362:Agar; D001435:Bacteriophages / Q000254:growth & development* / Q000302:isolation & purification*; D010948:Viral Plaque Assay / Q000295:instrumentation / Q000379:methods*
D000362:Agar