title: [Investigation into the controlled release behavior of gemcitabine-loaded nanovesicles].
abstract: OBJECTIVE
  To prepare gemcitabine-loaded nanovesicles and to observe its morphology, structure, particle size, and drug-release performance in vitro.
  
  
  METHODS
  Diemulsion technique was used to prepare nanovesicles as carrier from amphiphilic block copolymer of poly (ethylene glycol)-block-poly (D, L-lactide), and gemcitabine was used as the model drug. The morphology of vesicles was determined by scanning electron microscope (SEM) and transmission electron microscope (TEM), and its drug loading (DL), encapsulation ratio (ER), and drug-release curve in vitro were detected by UV-Vis-NIR Spectrophotometer.
  
  
  RESULTS
  The Gemcitabine-loaded nanovesicles is a kind of hollow nanosphere with the mean size of 200.6 nm, DL of 4.14% and ER of 20.54%. The nanovesicles showed its excellent controlled-release characteristic in the experiment of drug release in vitro.
  
  
  CONCLUSIONS
  The nanovesicles prepared from PEG-PDLLA can be served as one of carriers for Gemcitabine with good performance of drug controlled-release. It will provide a reliable experimental base for the further researches in vivo.
authors.affiliation: Department of Gastroenterology, First Municipal People's Hospital of Guangzhou, Guangzhou Medical College, Guangzhou 510180, China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Zheng
authors.forename: Lin
authors.initials: L
authors.lastname: Jia
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Kai-hong
authors.initials: KH
authors.lastname: Huang
authors.forename: Xin-tao
authors.initials: XT
authors.lastname: Shuai
authors.forename: Yu-yuan
authors.initials: YY
authors.lastname: Li
mesh_terms: D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D015195:Drug Design; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000493:pharmacokinetics; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D000093542:Gemcitabine
chemical_list: D003692:Delayed-Action Preparations; D007166:Immunosuppressive Agents; D003841:Deoxycytidine; D000093542:Gemcitabine