title: DNA typing of Diptera collected from human corpses in Portugal.
abstract: Medico-legal entomology, one area in the broad field of entomology, is routinely used in forensic applications. Insects are often collected from a corpse during criminal information related to the body, but requires the fast and accurate identification of the species attracted to the remains. The local entomofauna in most cases is important for explaining entomological evidence. The survey of the local entomofauna has become a fundamental first step in forensic entomological studies, because different geographical distributions, seasonal and environmental factors may influence the decomposition process and the occurrence of different species on corpses. A morphological and DNA-based methods for species identification were used in this study. Thirty-two cases are reported from indoors and outdoors conditions. Specimens were collected from corpses during autopsy procedures in the National Institute of Legal Medicine, Portugal, and cases were summarized by sex, death local, month of discovery, probable cause of death, species found and number of analyzed specimens. Just eight species, mainly Calliphoridae together with one Sarcophagidae were reported from corpses. The DNA sequencing was performed to study the mitochondrial encoded subunit I of the cytochrome oxidase gene. The aim of this work was the beginning of a database of the cadaveric entomofauna in Portugal.
authors.affiliation: Instituto Nacional Medicina Legal, Portugal. lcaine@dpinml.mj.pt
authors.forename: Laura M
authors.initials: LM
authors.lastname: Cainé
authors.forename: Francisco Corte
authors.initials: FC
authors.lastname: Real
authors.forename: Marta I
authors.initials: MI
authors.lastname: Saloña-Bordas
authors.forename: M Martínez
authors.initials: MM
authors.lastname: de Pancorbo
authors.forename: Gabiela
authors.initials: G
authors.lastname: Lima
authors.forename: Teresa
authors.initials: T
authors.lastname: Magalhães
authors.forename: Fátima
authors.initials: F
authors.lastname: Pinheiro
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D004772:Entomology; D004777:Environment; D005247:Feeding Behavior; D005260:Female; D018732:Forensic Anthropology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011174:Portugal; D011180:Postmortem Changes; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV