title: Hyperthermia on mesenchymal stem cells (MSCs) can sensitize tumor cells to undergo cell death.
abstract: Hyperthermia, the procedure of raising the temperature of tumor-loaded tissue to 40 degrees -43 degrees C, has been applied to various established cancer treatments. Although the mechanism of hyperthermia in cancer treatment is well-known, there are few or no studies regarding the effect of hyperthermia on the tumor-supportive stroma. Mesenchymal stem cells (MSCs) display the potential for differentiation into various tissues. MSCs are also reported to play a role as potential precursors for tumor stroma in providing a favorable environment for tumor progression. Here, we investigated the effects of hyperthermia-treated MSCs on the viability and growth of cancer cells. Culture supernatants from non-shocked or heat-shocked MSCs (NS-MSCs or HS-MSCs) were added to MCF7 cells. Morphological analysis and cell proliferation assay showed the reduced viability and growth of MCF7 cells by addition of culture medium conditioned by HS-MSCs. Additionally, exposure to the conditioned medium by HS-MSCs induced cell cycle arrest at G2/M phase, increased MHC class I, Fas receptor, and TNF-R expressions, and decreased MDR1 expression in the MCF7 cells. In particular, the conditioned medium of HS-MSCs accelerated the inhibition of tumor cell growth by several chemotherapeutic drugs. These data present new aspects of hyperthermia in cancer treatment, suggesting that hyperthermia can enable tumor stroma provide a sensitizing environment for tumor cells to undergo cell death.
authors.affiliation: Department of Biomedical Engineering, Korea University College of Health Science, Seoul, Korea.
authors.forename: Ho
authors.initials: H
authors.lastname: Park
authors.forename: Jung-Ah
authors.initials: JA
authors.lastname: Cho
authors.forename: Suel-Kee
authors.initials: SK
authors.lastname: Kim
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D002453:Cell Cycle; D016923:Cell Death; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017077:Culture Media, Conditioned; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D009363:Neoplasm Proteins / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins