title: [Association between single nucleotide polymorphism of MC4R gene and carcass traits in rabbits].
abstract: Single nucleotide polymorphisms in the coding sequence of melanoeortin-4 receptor (MC4R) gene were detected by PCR-SSCP and DNA sequencing method in Harbin white rabbit, Tianfu black rabbit, Belgian hare, ZIKA rabbit, and California rabbit breeds. A-->G conversion mutation at base position 237 was found with high frequency in Harbin white rabbit, Belgian hare, and Zika rabbit and low frequency in Tianfu black rabbit and California rabbit. The allele A was pre-dominant allele for each of meat rabbit breeds. AA genotype frequency was higher than AG genotype in the five studied rabbit breeds. GLM analysis for the effect of genotypes on performance traits demonstrated that AG genotype was significantly associated with body weight, eviscerated weight and feed conversion efficiency (P<0.05), but not significantly associated with cooking loss (P>0.05). It was concluded from the results that MC4R gene could be a candidate modifier gene that affects or controls body weight and carcass traits of rabbit.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China. jms.1699@163.com
authors.forename: Mei-Shan
authors.initials: MS
authors.lastname: Jiang
authors.forename: Shi-Yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Song-Jia
authors.initials: SJ
authors.lastname: Lai
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Deng
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Wan
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D011817:Rabbits; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D044105:Receptor, Melanocortin, Type 4