title: [Efficiency comparison between two preimplantation genetic diagnostic methods for chromosomal translocation carriers].
abstract: OBJECTIVE
  To compare the diagnostic efficiency between blastomere preimplantation genetic diagnosis (PGD) and polar body PGD for chromosomal translocation carriers.
  
  
  METHODS
  Group A had 8 cycles using whole painting probes for the first polar body diagnosis, while group B had 29 cycles using two subtelomeric probes and one centromeric probe for the blastomere diagnosis.
  
  
  RESULTS
  The fertilization rate of group A was significantly lower than group B [66.1% (72/109) vs 85.2% (304/357), P < 0.05]. There was no significant difference in the successful biopsy rate between two groups. However, group A had a significantly higher loss rate during fixation and higher no signal rate after fluorescence in situ hybridization [FISH, 9.6% (12/104) vs 1.6% (4/252), 11.2% (10/89) vs 3.0% (7/233)]. Totally, the diagnostic efficiency in group A (72.5%, 79/109) was significantly lower than that in group B (89.8%, 230/256, P < 0.05). Although both the clinical pregnancy rate (3/7) and implantation rate (22.2%, 4/18) of group A were higher, the differences were not statistically significant (P > 0.05).
  
  
  CONCLUSIONS
  Both methods can be used efficiently in the PGD for chromosomal translocation carriers. Blastomere PGD has a higher diagnostic rate.
authors.affiliation: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Xu
authors.forename: Xiu-Lian
authors.initials: XL
authors.lastname: Ren
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zeng
authors.forename: Cong
authors.initials: C
authors.lastname: Fang
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Can-Quan
authors.initials: CQ
authors.lastname: Zhou
authors.forename: Guang-Lun
authors.initials: GL
authors.lastname: Zhuang
mesh_terms: D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000166:cytology; D001757:Blastomeres / Q000166:cytology; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006580:Genetic Carrier Screening / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*; D012189:Retrospective Studies; D014178:Translocation, Genetic