SNARE-mediated fusion of liposomes.
Lipid-mixing assay is now commonly used to study protein, temperature and ion-dependent membrane fusion events. This assay has been crucial to demonstrate the ability of neuronal and non-neuronal soluble NSF attachment receptor (SNARE) to promote spontaneous fusion of liposomes. This lipid-mixing assay is based on the fluorescence resonance energy transfer (FRET) capability between a donor fluorescent lipid and a quenching lipid. When fusion between donor fluorescent liposomes and nonfluorescent acceptor liposome occurred, FRET decreases. This assay allows a real-time reading of SNARE-mediated liposome fusion.
Vicogne
Jérôme
J
Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.
Pessin
Jeffrey E
JE
D001681:Biological Assay; D015153:Blotting, Western; D006801:Humans; D008081:Liposomes / Q000302:isolation & purification / Q000378:metabolism*; D008561:Membrane Fusion; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / Q000302:isolation & purification / Q000378:metabolism*
D008081:Liposomes; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins