Hyperbaric oxygen for experimental intracerebral hemorrhage.
Acute brain edema formation contributes to brain injury after intracerebral hemorrhage (ICH). It has been reported that hyperbaric oxygen (HBO) is neuroprotective in cerebral ischemia, subarachnoid hemorrhage, and brain trauma. In this study, we investigated the effects of HBO on brain edema following ICH in rats. Male Sprague-Dawley rats received intracerebral infusion of autologous whole blood, thrombin, or ferrous iron. HBO (100% O2, 3.0 ATA for 1 h) was initiated 1 h after intracerebral injection. Control rats were exposed to air at room pressure. Brains were sampled at 24 or 72 h for water content, ion measurement, and Western blot analysis. We found that 1 session of HBO reduced perihematomal brain edema (p < 0.05) 24 h after ICH. HBO also reduced heat shock protein-32 (HSP-32) levels (p < 0.05) in ipsilateral basal ganglia 24h after ICH. However, HBO failed to attenuate thrombin-induced brain edema and exaggerated ferrous iron-induced brain edema (p < 0.05). Three sessions of HBO also failed to reduce brain edema 72h after ICH. In summary, HBO reduced early perihematomal brain edema and HSP-32 levels in brain. HBO-related brain protection does not occur through reduction in thrombin toxicity because HBO failed to attenuate thrombin-induced brain edema. Our results also indicate that HBO treatment after hematoma lysis for ICH may be harmful, since HBO amplifies iron-induced brain edema.
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Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109-2200, USA.
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Silbergleit
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D000704:Analysis of Variance; D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism / Q000473:pathology; D001777:Blood Coagulation / Q000502:physiology; D001929:Brain Edema / Q000209:etiology / Q000517:prevention & control; D002543:Cerebral Hemorrhage / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007501:Iron / Q000009:adverse effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism; D013917:Thrombin / Q000009:adverse effects; D013997:Time Factors
D012964:Sodium; D007501:Iron; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D013917:Thrombin