title: Enhanced expression of Hab18g/CD147 and activation of integrin pathway in HCC cells under 3-D co-culture conditions.
abstract: CD147 is reported to be correlated with the malignancy of some cancers, and its overexpression affects the progression of tumor. In the present study, we investigated the function of HAb18G/CD147, a member of CD147 family, on hepatocellular carcinoma (HCC) adhesion, invasion and metastasis in 3-dimensional (3-D) cell co-culture model. The results showed that the extracellular microenvironment could determine the cellular phenotypes and then affected the cellular functions. The expressions of HAb18G/CD147 in HCC cells and fibroblasts were both obviously elevated in 3-D co-culture model. The overexpression of HAb18G/CD147 increased MMPs' (MMP-2 and MMP-9) production (P < 0.01), and was obviously accompanied with enhanced expressions of paxillin, FAK and p-FAK in 3-D cell co-culture model. All the results suggest that HAb18G/CD147 plays an important role in HCC adhesion, invasion and metastasis mainly via modulating synthesis of MMPs and activating integrin signal pathways in fibroblasts and tumor cells themselves under the 3-D co-culture conditions.
authors.affiliation: Cell Engineering Research Centre & Department of Cell Biology, State Key Laboratory of Cancer Biology, The Fourth Military Medical University, 17 West Changle Road, Xi'an 710032, People's Republic of China.
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Wu
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.forename: Pu
authors.initials: P
authors.lastname: Zhao
authors.forename: Zhi-Nan
authors.initials: ZN
authors.lastname: Chen
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Jiang
mesh_terms: D000328:Adult; D051926:Basigin / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051419:Paxillin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D016023:Integrins; D051419:Paxillin; D051926:Basigin; D051416:Focal Adhesion Protein-Tyrosine Kinases; D020782:Matrix Metalloproteinases