title: Amplification and cloning of herpes simplex virus type 2 glycoprotein G from an Iranian isolate.
abstract: Herpes Simplex Virus type-2 (HSV-2) is the main cause of genital herpes infection. Its prevalence is increasing worldwide and varies widely with generally higher rate in developing than developed countries and urban than rural areas. HSV-2 Iranian isolate was propagated in HeLa cell line. The viral genome was extracted by phenol-chloroform and used as template in nested polymerase chain reactions (n-PCR) to amplify gG-2 gene. The amplified gene was cloned into a cloning vector (pTZ57R/T) and transformed into competent E. coli DH5a. The recombinant vector encoding the gene of interest was confirmed by restriction enzyme analysis and sequencing.
authors.affiliation: Department of Virology, Faculty of Medical Science, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Jamalidoost
authors.forename: Hoorieh
authors.initials: H
authors.lastname: Soleimanjahi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fotouhi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Meshkat
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D018258:Herpesvirus 2, Human / Q000235:genetics*; D006801:Humans; D007492:Iran; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2