Simultaneous analysis of anthocyanins and flavonols in petals of lotus (Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry.
A fast and reliable HPLC method for the simultaneous separation of anthocyanins and flavonols in lotus petals was developed based on the study of four candidate solvent systems. Fifteen flavonoids were identified by high-performance liquid chromatography with photodiode array detection/mass spectrometry. Among them, two anthocyanins and nine flavonols were discovered in lotus petals for the first time. This work is valuable for both the hybrid breeding on lotus oriented to flower color and the utilization of lotus petals as functional food materials.
Yang
Rui-Zhen
RZ
Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
Wei
Xiao-Lei
XL
Gao
Fen-Fang
FF
Wang
Liang-Sheng
LS
Zhang
Hui-Jin
HJ
Xu
Yan-Jun
YJ
Li
Chong-Hui
CH
Ge
Yu-Xuan
YX
Zhang
Jing-Jing
JJ
Zhang
Jie
J
D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D044948:Flavonols / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D000872:Anthocyanins; D044948:Flavonols; D010936:Plant Extracts