title: Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, part II: process optimization.
abstract: This work optimized the novel biotransformation process of podophyllotoxin to produce podophyllic acid by Pseudomonas aeruginosa CCTCC AB93066. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/l of yeast extract and 5 g/l of peptone were favorable for podophyllic acid production (i.e. 25.3+/-3.7 mg/l), while not beneficial for the cell growth of P. aeruginosa. This indicated that the accumulation of podophyllic acid was not corresponded well to the cell growth of P. aeruginosa. 0 g/l of sucrose was beneficial for podophyllic acid production (i.e. 34.3+/-3.9 mg/l), which led to high podophyllotoxin conversion (i.e. 98.2+/-0.1%). 1g/l of NaCl was the best for podophyllic acid production (i.e. 47.6+/-4.0mg/l). Secondly, the production of podophyllic acid was significantly enhanced by fed-batch biotransformation. When each 100mg/l of podophyllotoxin was added to the biotransformation system after 4, 10 and 25 h of culture, respectively, podophyllic acid concentration reached 99.9+/-12.3mg/l, enhanced by 284% comparing to one-time addition (i.e. 26.0+/-2.1mg/l). The fundamental information obtained in this study provides a simple and efficient way to produce podophyllic acid.
authors.affiliation: Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Mi
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Li
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Tang
mesh_terms: D000704:Analysis of Variance; D001711:Biotransformation / Q000187:drug effects; D007700:Kinetics; D009584:Nitrogen / Q000494:pharmacology; D010461:Peptones / Q000494:pharmacology; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012044:Regression Analysis; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects
chemical_list: D010461:Peptones; C507546:podophyllic acid; D012965:Sodium Chloride; D013395:Sucrose; D011034:Podophyllotoxin; D009584:Nitrogen