title: Engineered neuronal circuits shaped and interfaced with carbon nanotube microelectrode arrays.
abstract: Standard micro-fabrication techniques which were originally developed to fabricate semi-conducting electronic devices were inadvertently found to be adequate for bio-chip fabrication suited for applications such as stimulation and recording from neurons in-vitro as well as in-vivo. However, cell adhesion to conventional micro-chips is poor and chemical treatments are needed to facilitate the interaction between the device surface and the cells. Here we present novel carbon nanotube-based electrode arrays composed of cell-alluring carbon nanotube (CNT) islands. These play a double role of anchoring neurons directly and only onto the electrode sites (with no need for chemical treatments) and facilitating high fidelity electrical interfacing-recording and stimulation. This method presents an important step towards building nano-based neurochips of precisely engineered networks. These neurochips can provide unique platform for studying the activity patterns of ordered networks as well as for testing the effects of network damage and methods of network repair.
authors.affiliation: School of Electrical Engineering, Tel-Aviv University, Tel-Aviv 69978, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Shein
authors.forename: A
authors.initials: A
authors.lastname: Greenbaum
authors.forename: T
authors.initials: T
authors.lastname: Gabay
authors.forename: R
authors.initials: R
authors.lastname: Sorkin
authors.forename: M
authors.initials: M
authors.lastname: David-Pur
authors.forename: E
authors.initials: E
authors.lastname: Ben-Jacob
authors.forename: Y
authors.initials: Y
authors.lastname: Hanein
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology*; D004567:Electrodes, Implanted; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D009924:Organ Culture Techniques / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D037742:Nanotubes, Carbon