A new therapeutic modality involving acridine orange excitation by photon energy used during reduction surgery for rhabdomyosarcomas.
Rhabdomyosarcoma is a common malignant soft tissue that frequently involves bone and major neurovascular structures and resection of deep-seated rhabdomyosarcoma can cause severe dysfunction in the affected limbs. Based on the mouse osteosarcoma model, we developed a new surgical approach involving photodynamic surgery (PDS), photodynamic therapy (PDT) and radiodynamic therapy (RDT) using acridine orange (AO). Six rhabdomyosarcoma cases were treated using this new modality after confirming the effectiveness of AO-PDT on human rhabdomyosarcoma cell lines. All patients had almost normal limb function after surgery, with only one recurrence. Based on these results, AO-PDS, PDT and RDT can be used to preserve excellent limb function in patients with rhabdomyosarcoma involving major nerves and vessels or bones.
Matsubara
Takao
T
Department of Orthopaedic Surgery, Mie Postgraduate School of Medicine, Mie Prefecture 514-8507, Japan.
Kusuzaki
Katsuyuki
K
Matsumine
Akihiko
A
Murata
Hiroaki
H
Satonaka
Haruhiko
H
Shintani
Ken
K
Nakamura
Tomoki
T
Hosoi
Hajime
H
Iehara
Tomoko
T
Sugimoto
Toru
T
Uchida
Atsumasa
A
D000165:Acridine Orange / Q000627:therapeutic use*; D000293:Adolescent; D045744:Cell Line, Tumor; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D017785:Photons / Q000627:therapeutic use*; D012208:Rhabdomyosarcoma / Q000473:pathology / Q000628:therapy*; D012983:Soft Tissue Neoplasms / Q000473:pathology / Q000628:therapy
D000165:Acridine Orange