title: Adaptation of myosin heavy chain mRNA expression after implantation of poly(3)hydroxybutyrate scaffolds in rat m. latissimus dorsi.
abstract: The aim of this study is to identify the synergistic effect between an ectopic bone substitute and surrounding tissues, in this case muscle tissue, which is known to have a considerable potential for adaptation. To describe this effect, changes of myosin heavy chain (MyHC) isoform mRNA content of 12 Wistar-King rats m. latissimus dorsi with implanted poly(3)hydroxybutyrate (PHB) scaffolds were examined after six and 12 weeks. At each time interval six rats were killed and implants and surrounding tissues prepared for genetic evaluation. Eight rats without any implants served as controls. After homogenisation of muscle tissue, RNA was extracted and reverse transcribed. Changes in mRNA content were measured by Real-Time PCR using specific primers for type I MyHC, IIa, IIb and IIx isoforms. The mRNA level of myosin isoform type I of the muscles surrounding the implant was significantly increased (p<0.02) compared to the control group. Further, the studied muscle tissue showed a significant decrease in MyHC isoform IIx mRNA compared to the controls (p<0.02). Implantation of PHB scaffolds into rat m. latissimus dorsi causes an increase of its' content of slow myosin isoforms indicating a synergistic effect between the PHB scaffold and the surrounding muscle tissue.
authors.affiliation: Department of Orthodontics, Preventive and Paediatric Dentistry, Ernst-Moritz-Arndt University Greifswald, Germany. h.mack@griffith.edu.au
authors.forename: H B
authors.initials: HB
authors.lastname: Mack
authors.forename: R
authors.initials: R
authors.lastname: Mai
authors.forename: G
authors.initials: G
authors.lastname: Lauer
authors.forename: F
authors.initials: F
authors.lastname: Mack
authors.forename: T
authors.initials: T
authors.lastname: Gedrange
authors.forename: R
authors.initials: R
authors.lastname: Franke
authors.forename: T
authors.initials: T
authors.lastname: Gredes
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D003001:Cloning, Molecular; D006885:Hydroxybutyrates; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000096:biosynthesis*; D011091:Polyesters; D000091402:Prohibitins; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D000094122:Polyhydroxybutyrates
chemical_list: D018786:Bone Substitutes; D006885:Hydroxybutyrates; D018995:Myosin Heavy Chains; D011091:Polyesters; D000091402:Prohibitins; D012333:RNA, Messenger; C000717332:Phb protein, rat; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates