In vivo response of Mesocestoides vogae to human insulin.
Successful host invasion by parasitic helminths involves detection and appropriate response to a range of host-derived signals. Insulin signal response pathways are ancient and highly-conserved throughout the metazoans. However, very little is known about helminth insulin signalling and the potential role it may play in host-parasite interactions. The response of Mesocestoides vogae (Cestoda: Cyclophyllidea) larvae to human insulin was investigated, focusing on tyrosine-phosphorylation status, glucose content, survival and asexual reproduction rate. Parasite larvae were challenged with different levels of insulin for variable periods. The parameters tested were influenced by human insulin, and suggested a host-parasite molecular dialogue.
Canclini
L
L
Department of Cellular and Molecular Biology, Faculty of Sciences, University of the Republic of Uruguay, Iguá, Montevideo, Uruguay.
Esteves
A
A
D000595:Amino Acid Sequence; D000818:Animals; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008645:Mesocestoides / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D012100:Reproduction, Asexual; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism
D007328:Insulin; D014443:Tyrosine; D005947:Glucose