Can Hg(II) be determined via quenching of the emission of green fluorescent protein from Anemonia sulcata var. smaragdina?
Anemonia sulcata var. smaragdina is a widely distributed Cnidarian species along Turkish coastlines. It is also a well-known example of a facultative symbiotic life form in sea ecosystems. Green fluorescent proteins (GFPs) in Anemonia sulcata var. smaragdina have vital roles in this symbiotic form. The fluorescence quenching by Hg(II) in the supernatants obtained from A. sulcata var. smaragdina was shown in this study. According to results, there was a statistical significant relationship (R(2) = 0.9913) between increased Hg(II) concentration and decreased fluorescence intensity of GFP supernatants obtained from A. sulcata var. smaragdina. Mn(II), Fe(II), and Al(II) showed no interference effect and did not change the fluorescence intensity of GFP supernatants obtained from A. sulcata var. smaragdina. In conclusion, the fluorescence quenching of GFPs by Hg(II) can be a novel method to determine the Hg(II) levels in aqueous solution. Therefore, further researches are strongly warranted because of the possible potential applications of the fluorescence quenching of GFPs by Hg(II).
Bozkurt
Serap Seyhan
SS
Faculty of Arts and Sciences, Department of Chemistry, Division of Analytical Chemistry, Dokuz Eylul University, Izmir, Turkey.
Cavas
Levent
L
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D016014:Linear Models; D008628:Mercury / Q000032:analysis* / Q000633:toxicity*; D012615:Sea Anemones / Q000187:drug effects* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
D014867:Water; D049452:Green Fluorescent Proteins; D008628:Mercury