Suitability of human mesenchymal stem cells for gene therapy depends on the expansion medium.
Great hope is set in the use of mesenchymal stem cells for gene therapy and regenerative medicine. Since the frequency of this subpopulation of stem cells in bone marrow is low, mesenchymal stem cells are expanded ex vivo and manipulated prior to experimental or clinical use. Different methods for isolation and expansion are available, but the particular effect on the stem cell character is unclear. While the isolation of mesenchymal stem cells by density centrifugation followed by selection of the plastic adherent fraction is frequently used, the composition of expansion media differs. Thus, in the present study we cultured mesenchymal stem cells isolated from five healthy young volunteers in three widely used expansion media and performed a detailed analysis of the effect on morphology, proliferation, clonogenicity, passaging, differentiation and senescence. By this way we clearly show that the type of expansion medium used determines the stem cell character and time of senescence which is critical for future gene therapeutic and regenerative approaches using mesenchymal stem cells.
Apel
Anja
A
Molecular OncoSurgery Group, University of Heidelberg and German Cancer Research Center, Heidelberg, Germany.
Groth
Ariane
A
Schlesinger
Sabine
S
Bruns
Helge
H
Schemmer
Peter
P
Büchler
Markus W
MW
Herr
Ingrid
I
D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D055815:Young Adult
D003470:Culture Media