title: Identification of a new molecular target of class IIa bacteriocins in Listeria monocytogenes EGDe.
abstract: It was shown recently (Calvez et al. 2007) that glpQ and pde genes are involved in intermediate resistance of Enterococcus faecalis JH2-2 to DvnV41, a class IIa bacteriocin produced by Carnobacterium divergens V41. The listerial orthologs of lpQ and pde genes might be lmo0052 and lmo1292 genes, respectively. Here, the role of lmo0052 and lmo1292 genes in resistance of Listeria monocytogenes EGDe to DvnV41 and MesY105 was investigated. L. monocytogenes EGDe was inactivated in lmo0052 and/or lmo1292 by homologous recombination. Listerial mutant strain EGDSC02 (inactivated in the putative glpQ gene), was slightly resistant to DvnV41. The listerial mutant strain EGDSC01 (inactivated in the putative pde gene) remained, as the wild-type strain, sensitive to DvnV41, but was affected in growth parameters.
authors.affiliation: Laboratoire de Microbiologie, Ecole Nationale d'Ingénieurs des Techniques des Industries Agricoles et Alimentaires, 44322, Nantes Cedex 3, France.
authors.forename: S
authors.initials: S
authors.lastname: Calvez
authors.forename: H
authors.initials: H
authors.lastname: Prévost
authors.forename: D
authors.initials: D
authors.lastname: Drider
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D010727:Phosphoric Diester Hydrolases; C087921:glpQ protein, Bacteria