title: Comparison of luminescent immunoassays using biotinylated proteins of aequorin, alkaline phosphatase and horseradish peroxidase as reporters.
abstract: We compare aequorin, alkaline phosphatase and horseradish peroxidase as reporters for luminescent immunoassays using alpha-fetoprotein (AFP) as a model analyte. Biotinylated aequorin was prepared from mutated aequorin containing a reactive cysteine residue by chemical conjugation. The measurable range of AFP using this biotinylated aequorin was 0.02-200 ng/ml, with a lower background level than the other biotinylated enzymes tested.
authors.affiliation: Yokohama Research Center, Chisso Corporation, Yokohama, Japan. sinouye@chisso.co.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inouye
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Sato
mesh_terms: D000331:Aequorin / Q000032:analysis / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D019921:Biotinylation; D006735:Horseradish Peroxidase / Q000032:analysis / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D000509:alpha-Fetoproteins; D000331:Aequorin; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase