Co-production of two new peptide antibiotics by a bacterial isolate Paenibacillus alvei NP75.
Two new peptide antibiotics were secreted by a Gram-positive bacterial strain isolated from fermented tomato fruit. Based on its 99% 16S rDNA sequence similarity with Paenibacillus alvei, the isolate was designated as P. alvei NP75. Among these two peptides, one is active against Gram-positive pathogens while the other against Gram-negative pathogens; thus these peptides were named as paenibacillin P and paenibacillin N, respectively. After the purification of those peptide antibiotics from the cell free culture supernatant by RP-HPLC, they were analyzed for their temperature sensitivity and susceptibility to proteases. Higher-temperature tolerant paenibacillin N was easily degraded by proteinase K, while the temperature sensitive paenibacillin P was not affected by any of the proteases used in this study other than a specific protease that was secreted by the same NP75 strain. Mass-spectrometry analysis of the above peptide antibiotics further confirmed their distinction among the known peptide antibiotics. We are reporting first of its kind the co-production of two different new peptide antibiotics from a single bacterial isolate of P. alvei strain.
Anandaraj
Balaiah
B
Genetic Engineering Unit, Centre for Biotechnology, Anna University, Sardar Patel Road, Guindy, Chennai 600 025, India.
Vellaichamy
Adaikkalam
A
Kachman
Maureen
M
Selvamanikandan
Athinarayanan
A
Pegu
Shyamanta
S
Murugan
Vadivel
V
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D018551:Solanum lycopersicum / Q000382:microbiology; D013058:Mass Spectrometry
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; C535070:paenibacillin N; C535069:paenibacillin P