An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules.
Toward the expansion of the genetic alphabet, we present an unnatural base pair system for efficient PCR amplification, enabling the site-specific incorporation of extra functional components into DNA. This system can be applied to conventional PCR protocols employing DNA templates containing unnatural bases, natural and unnatural base triphosphates, and a 3'-->5' exonuclease-proficient DNA polymerase. For highly faithful and efficient PCR amplification involving the unnatural base pairing, we identified the natural-base sequences surrounding the unnatural bases in DNA templates by an in vitro selection technique, using a DNA library containing the unnatural base. The system facilitates the site-specific incorporation of a variety of modified unnatural bases, linked with functional groups of interest, into amplified DNA. DNA fragments (0.15 amol) containing the unnatural base pair can be amplified 10(7)-fold by 30 cycles of PCR, with <1% total mutation rate of the unnatural base pair site. Using the system, we demonstrated efficient PCR amplification and functionalization of DNA fragments for the extremely sensitive detection of zeptomol-scale target DNA molecules from mixtures with excess amounts (pmol scale) of foreign DNA species. This unnatural base pair system will be applicable to a wide range of DNA/RNA-based technologies.
Kimoto
Michiko
M
Systems and Structural Biology Center, RIKEN, Yokohama, Kanagawa 230-0045, Japan.
Kawai
Rie
R
Mitsui
Tsuneo
T
Yokoyama
Shigeyuki
S
Hirao
Ichiro
I
D000480:Alkynes / Q000737:chemistry*; D020029:Base Pairing; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011725:Pyridines / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
C528341:7-(2-thienyl)imidazo(4,5-b)pyridine; D000480:Alkynes; D007093:Imidazoles; D009711:Nucleotides; D011725:Pyridines; D011758:Pyrroles; D004247:DNA