title: Association of the cholesteryl ester transfer protein Taq1 B2B2 genotype with higher high-density lipoprotein cholesterol concentrations and lower risk of coronary artery disease in a Tunisian population.
abstract: BACKGROUND
  The role of cholesteryl ester transfer protein (CETP) in the development of atherosclerosis is undergoing debate.
  
  
  OBJECTIVE
  In this prospective study, we sought to explore the role of the CETP Taq1B variant in coronary artery disease risk, and its association with plasma lipid and apolipoprotein concentrations.
  
  
  METHODS
  DNA was extracted from 316 patients undergoing coronary angiography. The Taq1B polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis. Lipid and apolipoprotein concentrations were measured by enzymatic and nephelometric assays.
  
  
  RESULTS
  In our study population, the B2 allele frequency was 0.29. B2 allele carriers had a significantly higher high-density lipoprotein cholesterol (HDL-C) concentration than those with the B1B1 genotype (1.041+/-0.294 versus 0.995+/-0.277; p=0.039). After adjusting for age, sex, smoking status, diabetes, hypertension and dyslipidaemia, the odds ratio (OR) for significant stenosis associated with the B2 allele was 0.82 (95% confidence interval (CI) 0.60-0.97; p=0.039), suggesting that the B2 allele is associated with an 18% lower risk of significant stenosis. This protective effect seemed to be more significant in male nonsmokers (38% lower risk; OR 0.62, 95% CI 0.29-0.92; p=0.029). No significant protective effects were observed in women or male smokers.
  
  
  CONCLUSIONS
  Our data suggest that the B2 allele is associated with higher concentrations of HDL-C, which confer a protective effect with regard to coronary atherosclerosis. This effect seems to be more significant in men than in women and in nonsmokers than in smokers.
authors.affiliation: Biochemistry Department, UR MSP28/04, Sahloul University Hospital, 4054 Sousse, Tunisia.
authors.forename: Jihène
authors.initials: J
authors.lastname: Rejeb
authors.forename: Asma
authors.initials: A
authors.lastname: Omezzine
authors.forename: Lamia
authors.initials: L
authors.lastname: Rebhi
authors.forename: Ilyes
authors.initials: I
authors.lastname: Naffeti
authors.forename: Kaouther
authors.initials: K
authors.lastname: Kchok
authors.forename: Radhia
authors.initials: R
authors.lastname: Belkahla
authors.forename: Ibtihel
authors.initials: I
authors.lastname: Bel Hadjmbarek
authors.forename: Nabila
authors.initials: N
authors.lastname: Ben Rejeb
authors.forename: Naoufel
authors.initials: N
authors.lastname: Nabli
authors.forename: Amira
authors.initials: A
authors.lastname: Boujelbene
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ben Abdelaziz
authors.forename: Essia
authors.initials: E
authors.lastname: Boughzala
authors.forename: Ali
authors.initials: A
authors.lastname: Bouslama
mesh_terms: D000368:Aged; D000483:Alleles; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D003324:Coronary Artery Disease / Q000097:blood / Q000453:epidemiology*; D023921:Coronary Stenosis / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D012737:Sex Factors; D012907:Smoking / Q000097:blood / Q000453:epidemiology; D014416:Tunisia / Q000453:epidemiology
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D008075:Lipoproteins, HDL