title: Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group.
abstract: The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.
authors.affiliation: Institute of Legal Medicine, Innsbruck Medical University, Müllerstr. 44, 6020 Innsbruck, Austria. walther.parson@i-med.ac.at
authors.forename: Walther
authors.initials: W
authors.lastname: Parson
authors.forename: Harald
authors.initials: H
authors.lastname: Niederstätter
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lindinger
authors.forename: Peter
authors.initials: P
authors.lastname: Gill
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry; D041322:Chromosomes, Human, Y; D045424:Complex Mixtures / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000592:standards*; D004279:DNA, Viral; D053803:Forensic Genetics; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013048:Specimen Handling
chemical_list: D045424:Complex Mixtures; D004279:DNA, Viral; D004247:DNA