Enhanced secretion of biologically active murine interleukin-12 by Lactococcus lactis.
The novel signal peptide SLPmod was used for the secretion of murine interleukin-12 (mIL-12) by Lactococcus lactis. A >4-fold increase in secretion was observed when SLPmod was used instead of the Usp45-derived secretion signal. Oral delivery of this cytokine using the autoinducible host L. lactis FI5876 utilizing SLPmod resulted in a significant increase in mIL-12 plasma levels in mice.
Fernandez
Antonio
A
BBSRC Institute of Food Research, Colney, Norwich, United Kingdom. antonio.fernandezdumont@efsa.europa.eu
Horn
Nikki
N
Wegmann
Udo
U
Nicoletti
Claudio
C
Gasson
Michael J
MJ
Narbad
Arjan
A
D000818:Animals; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010949:Plasma / Q000737:chemistry; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D018664:Interleukin-12