title: Derived chromatographic indices as effective tools to study the self-aggregation process of bile acids.
abstract: Reversed-phase high-performance liquid chromatography (RP-HPLC) is more and more adopted as a fruitful tool for the estimation of several physico-chemical properties of diverse classes of organic compounds. In this frame, derived chromatographic indices have been proposed as effective parameters to measure the lipophilicity (log P or log D) of compounds. Instead, a limited attention has been directed towards the chromatographic evaluation of the Critical Micellar Concentration (CMC), one of the most important parameters employed to study the bile acid physico-chemical profile. We have recently reported on the effectiveness of the derived chromatographic index phi(0) for the study of the self-aggregation process of bile acids. Here we show that this index is independent upon the adopted chromatographic environment so as to be instrumental for the evaluation of the hydrophobic/hydrophilic balance of bile acids. Molecular modelling studies have also been undertaken with the aim of rationalizing the experimental findings.
authors.affiliation: Università degli Studi di Perugia, Dipartimento di Chimica e Tecnologia del Farmaco, Via del Liceo, 1, 06123 Perugia, Italy. natalini@chimfarm.unipg.it
authors.forename: Benedetto
authors.initials: B
authors.lastname: Natalini
authors.forename: Roccaldo
authors.initials: R
authors.lastname: Sardella
authors.forename: Emidio
authors.initials: E
authors.lastname: Camaioni
authors.forename: Antonio
authors.initials: A
authors.lastname: Macchiarulo
authors.forename: Antimo
authors.initials: A
authors.lastname: Gioiello
authors.forename: Giovanni
authors.initials: G
authors.lastname: Carbone
authors.forename: Roberto
authors.initials: R
authors.lastname: Pellicciari
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D016014:Linear Models; D008823:Micelles; D008958:Models, Molecular
chemical_list: D001647:Bile Acids and Salts; D008823:Micelles