Enzyme assays.
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign production processes. The present article highlights the aspects of fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting, which are our particular areas of interest.
Reymond
Jean-Louis
JL
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, Berne, 3012, Switzerland. jean-louis.reymond@ioc.unibe.ch
Fluxà
Viviana S
VS
Maillard
Noélie
N
D000818:Animals; D001709:Biotechnology / Q000191:economics / Q000639:trends*; D004396:Coloring Agents; D004798:Enzymes / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007202:Indicators and Reagents; D036103:Nanotechnology; D010449:Peptide Mapping
D004396:Coloring Agents; D004798:Enzymes; D007202:Indicators and Reagents