[The role of extracellular chaperone Hsp70 in creating antitumor immunity in rat rhabdomyosarcoma RA-2 model].
Immunization of experimental animals with extract or membranes of rat rhabdomyosarcoma RA-2 in combination with pure Hsp70 did not offer any significant effect of protection from subsequent RA-2 cells-stimulated tumor growth. By contrast, immunization with preparations of pure Hsp70 led to a significant decrease in number and size of tumors as well as elevation of concentrations of antibodies against RA-2 cells. Also, enhanced blood levels of Hsp70 involved delayed tumor growth. In vitro tests Hsp70 incubation with RA-2 cells was followed by a 30-35% rise in cytotoxic lymphocytes levels. An ability of pure Hsp70 preparations to stimulate humoral and antitumor response was demonstrated. Hence, they may be used in developing vaccine formulas.
Guzhova
I V
IV
Komarova
E Iu
EIu
Pimenova
A A
AA
Bakhtin
Iu B
IuB
Kaminskaia
E V
EV
Margulis
B A
BA
D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D012208:Rhabdomyosarcoma / Q000276:immunology*
D019496:Cancer Vaccines; D018840:HSP70 Heat-Shock Proteins