title: Complete nucleotide sequence of a Polish strain of Peanut stunt virus (PSV-P) that is related to but not a typical member of subgroup I.
abstract: Peanut stunt virus (PSV) is a common legume pathogen present worldwide. It is also infectious for many other plants including peanut and some vegetables. Viruses of this species are classified at present into three subgroups based on their serology and nucleotide homology. Some of them may also carry an additional subviral element - satellite RNA. Analysis of the full genome sequence of a Polish strain - PSV-P - associated with satRNA was performed and showed that it may be classified as a derivative of the subgroup I sharing 83.9-87.9% nucleotide homology with other members of this subgroup. A comparative study of sequenced PSV strains indicates that PSV-P shows the highest identity level with PSV-ER or PSV-J depending on the region used for analysis. Phylogenetic analyses, on the other hand, have revealed that PSV-P is related to representatives of the subgroup I to the same degree, with the exception of the coat protein coding sequence where PSV-P is clustered together with PSV-ER.
authors.affiliation: Interdepartmental Laboratory of Molecular Biology - National Research Institute, Poznań, Poland. ao.steplowska@ior.poznan.pl
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Obrepalska-Steplowska
authors.forename: Marta
authors.initials: M
authors.lastname: Budziszewska
authors.forename: Henryk
authors.initials: H
authors.lastname: Pospieszny
mesh_terms: D001483:Base Sequence; D017799:Cucumovirus / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D005814:Genes, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers