A quantitative and morphometric study of mast cells in cutaneous leishmaniasis.
Background Mast cells (MCs) are related with healing process in chronic inflammatory diseases, although in cutaneous leishmaniasis (CL) its importance is unknown. The aim of this study was to determine the correlation of MC with clinical findings in patients with the localized form of CL. Methods A cohort of 85 patients with CL was evaluated. MCs count was performed in pre-treatment biopsies and correlation with clinical findings and Leishmania species determined by PCR were performed. Results The MCs count in patients with CL caused by Leishmania (V.) braziliensis was 14.3 +/- 9.8 cells/mm(2), and 7.0 +/- 6.5 cells/mm(2) in patients with L. (L.) amazonensis (P < 0.05). The linear regression of MCs count with the age showed a tendency of cell number decreasing, according to ageing of the patient (r2 = 0.05; P < 0.05). The association of disease's duration and MCs count was positive (r2 = 0.11; P < 0.05). There was not any association of MCs count with number of lesions neither with Leishmania antigen expression. The MCs count was higher in patients with earlier healing after treatment (P < 0.05). Conclusion MC can be important in CL and related with healing lesion.
Tuon
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Department of Infectious and Parasitic Diseases, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
Duarte
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MI
Amato
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VS
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000818:Animals; D001706:Biopsy; D015331:Cohort Studies; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000473:pathology*; D007958:Leukocyte Count; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000473:pathology; D013223:Statistics as Topic; D013997:Time Factors
D016054:DNA, Protozoan