[Homing of CFSE-labeled antigen-specific CD4(+)CD25(+) regulatory T cells in islets transplantation].
OBJECTIVE
To observe the distribution and proliferation of the antigen-specific CD4(+)CD25(+) regulatory T (Treg ) cells and investigate the potential mechanism of antigen-specific CD4(+)CD25(+) regulatory T cells on the suppression of rejection for allogenetic islet transplantation in vivo.


METHODS
Antigen-specific CD4(+)CD25(+) Treg cells were generated by the addition of multiple intravenous injections of ICR mice splenocytes in vivo. After labeled by CFSE, 8x10(5) antigen-specific Treg cells were injected via tail vein with islets transplantation. Homing and distribution of antigen-specific CD4(+)CD25(+) Treg cells were investigated by flow cytometric analysis and immunofluorescence.


RESULTS
In vivo, the mean survival time of recipients with islets and antigen-specific CD4(+)CD25(+) Treg cells were (34.57+/-17.15) days, whereas transplanted islets without Treg treatment survived (10.6+/-1.82) days in control mice. The transferred antigen-specific CD4(+)CD25(+) Treg cells were mainly resident in pancreatic node and mesenteric node.


CONCLUSIONS
Allogeneic antigen-specific CD4(+)CD25(+) Treg cells prolong islet graft survival, and draining lymphoid tissue play a key role in the immune response.
Zhang
Mei
M
Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Xu
Shu-Hang
SH
Xu
Yu
Y
Liu
Cui-Ping
CP
Mao
Xiao-Dong
XD
Xu
Kuan-Feng
KF
Liu
Chao
C
D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005452:Fluoresceins / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D013388:Succinimides / Q000737:chemistry*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*; D014184:Transplantation, Homologous / Q000276:immunology
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D015704:CD4 Antigens; D005452:Fluoresceins; D053645:Interleukin-2 Receptor alpha Subunit; D013388:Succinimides