title: Microfabricated grooved substrates influence cell-cell communication and osteoblast differentiation in vitro.
abstract: Implant topography influences osteointegration, but the architectural parameters that dictate cell behavior and the molecular mechanisms remain unclear. Polycarbonate grooved substrates 10, 15, or 30 microm wide and 7 microm deep with an inter-groove distance of 2 microm were contrasted for their ability to influence osteoblasts in vitro. Osteoblasts grew well on the substrates and became alkaline phosphatase-positive under osteogenic conditions, although extensive mineralization of bone-like nodules was only observed on flat surfaces. Osteoblasts were aligned according to the grooves, and on 10-microm grooves had a significantly smaller area than on other surfaces. Vinculin staining revealed that there were significantly smaller and fewer focal adhesions per cell on the grooves than on the flat surfaces. Localization of the gap junction protein, connexin-43, showed lower expression in cells on the 10-microm grooves with lower frequency of large (>3 microm(2)) gap junction complexes. Cell-cell communication was assessed using fluorescence recovery after photobleaching, demonstrating that cells on the 10-microm grooves exhibited significantly slower dye uptake after bleaching. Cells in the layers above the grooves also had slower dye recovery times. Maintaining appropriate cell-cell communication structures is pivotal in the process of osteoblast differentiation, and the design of orthopedic biomaterials should ensure that interacting cells can maintain these critical interactions.
authors.affiliation: Musculoskeletal Research Group, Institute for Cellular Medicine, The Medical School, Newcastle University, Newcastle upon Tyne, United Kingdom.
authors.forename: George
authors.initials: G
authors.lastname: Kirmizidis
authors.forename: Mark A
authors.initials: MA
authors.lastname: Birch
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D018031:Connexin 43 / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism; D008422:Materials Testing / Q000379:methods*; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000187:drug effects; D011076:Polycarboxylate Cement / Q000494:pharmacology*; D051381:Rats; D012886:Skull / Q000166:cytology
chemical_list: D018031:Connexin 43; D011076:Polycarboxylate Cement; C005506:polycarbonate