Micronucleus analysis in patients with colorectal adenocarcinoma and colorectal polyps.
OBJECTIVE
To determine, by counting micronucleus (MN) frequencies, whether chromosomal or DNA damage have an effect on the pathogenesis of early colorectal adenocarcinoma (CRC).


METHODS
We analyzed MN frequencies in 21 patients with CRC, 24 patients with colon polyps [10 neoplastic polyps (NP) and 14 non-neoplastic polyps (NNP)] and 20 normal controls.


RESULTS
MN frequency was significantly increased in CRC patients and in NP patients compared with controls (3.72 +/- 1.34, 3.58 +/- 1.21 vs 1.97 +/- 0.81, P < 0.001). However, there was no difference in the MN frequency between CRC patients and NP patients (P > 0.05). Similarly, there was no difference in the MN frequency between NNP patients (2.06 +/- 0.85) and controls (P > 0.05).


CONCLUSIONS
Our results suggest increased chromosome/DNA instabilities may be associated with the pathogenesis of early CRC.
Karaman
Ali
A
Erzurum Training and Research Hospital, Department of Medical Genetics, Erzurum 25240, Turkey. alikaramandr@hotmail.com
Binici
Doğan Nasir
DN
Kabalar
Mehmet Eşref
ME
Calikuşu
Züleyha
Z
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002478:Cells, Cultured; D003111:Colonic Polyps / Q000235:genetics* / Q000473:pathology; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D042822:Genomic Instability; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology