The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections.
The aim of this study was to compare the performance of four different ELISA procedures in diagnosing Trichinella spp. infections in human outbreaks. The main differences between the various procedures were: antigen concentration, dilutions of human serum sample, the type of conjugate used, and time of conjugate incubation. Serum samples were collected from 23 individuals involved in an outbreak. Results obtained in the four tested ELISA procedures differed in the rate of positive results obtained for the examined outbreak. Western blot confirmed the ELISA-positive results. Reactions to the specific 41-45kDa and 55kDa bands were obtained with all positive sera.
Moskwa
B
B
Witold Stefanski Institute of Parasitology of the Polish Academy of Sciences, Twarda Street 51/55, Warsaw, Poland. moskwa@twarda.pan.pl
Bien
J
J
Cabaj
W
W
Korinková
K
K
Koudela
B
B
Stefaniak
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002648:Child; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D012698:Serologic Tests / Q000379:methods; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology* / Q000469:parasitology; D055815:Young Adult
D000909:Antibodies, Helminth; D007074:Immunoglobulin G