Cytolytic assessment of hyperacute rejection and production of nuclear transfer embryos using hCD46-transgenic porcine embryonic germ cells.
Human complement regulatory protein hCD46 may reduce the hyperacute rejection (HAR) in pig-to-human xenotransplantation. In this study, an hCD46 gene was introduced into porcine embryonic germ (EG) cells. Treatment of human serum did not affect the survival of hCD46-transgenic EG cells, whereas the treatment significantly reduced the survival of non-transgenic EG cells (p < 0.01). The transgenic EG cells presumably capable of alleviating HAR were transferred into enucleated oocytes. Among 235 reconstituted oocytes, 35 (14.9%) developed to the blastocyst stage. Analysis of individual embryos indicated that 80.0% (28/35) of embryos contained the transgene hCD46. The result of the present study demonstrates resistance of hCD46-transgenic EG cells against HAR, and the usefulness of the transgenic approach may be predicted by this cytolytic assessment prior to actual production of transgenic pigs. Subsequently performed EG cell nuclear transfer gave rise to hCD46-transgenic embryos. Further study on the transfer of these embryos to recipients may produce hCD46-transgenic pigs.
Won
Ji Young
JY
Department of Physiology, Dankook University School of Medicine, Cheonan, Korea.
Ahn
Kwang Sung
KS
Sorrell
Alice M
AM
Shin
Susa
S
Heo
Soon Young
SY
Kang
Jee Hyun
JH
Park
Jin-Ki
JK
Chang
Won-Kyong
WK
Shim
Hosup
H
D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000502:physiology*; D047108:Embryonic Development; D018014:Gene Transfer Techniques; D006801:Humans; D051916:Membrane Cofactor Protein / Q000235:genetics* / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology*; D013552:Swine / Q000235:genetics*; D019076:Transgenes
C497857:CD46 protein, human; D051916:Membrane Cofactor Protein