title: A study on the interactions of Aurein 2.5 with bacterial membranes.
abstract: Aurein 2.5 (GLFDIVKKVVGAFGSL-NH(2)) is an uncharacterised antimicrobial peptide. At an air/water interface, it exhibited strong surface activity (maximal surface pressure 25mNm(-1)) and molecular areas consistent with the adoption of alpha-helical structure orientated either perpendicular (1.72nm(2)molecule(-1)) or parallel (3.6nm(2)molecule(-1)) to the interface. Aurein 2.5 was strongly antibacterial, exhibiting a minimum inhibitory concentration (MIC) of 30microM against Bacillus subtilis and Escherichia coli. The peptide induced maximal surface pressure changes of 9mNm(-1) and 5mNm(-1), respectively, in monolayers mimicking membranes of these organisms whilst compression isotherm analysis of these monolayers showed DeltaG(Mix)>0, indicating destabilisation by Aurein 2.5. These combined data suggested that toxicity of the peptide to these organisms may involve membrane invasion via the use of oblique orientated alpha-helical structure. The peptide induced strong, comparable maximal surface changes in monolayers of DOPG (7.5mNm(-1)) and DOPE monolayers (6mNm(-1)) suggesting that the membrane interactions of Aurein 2.5 were driven by amphiphilicity rather than electrostatic interaction. Based on these data, it was suggested that the differing ability of Aurein 2.5 to insert into membranes of B. subtilis and E. coli was probably related to membrane-based factors such as differences in lipid packing characteristics. The peptide was active against both sessile E. coli and Staphylococcus aureus with an MIC of 125microM. The broad-spectrum antibacterial activity and non-specific modes of membrane action used by Aurein 2.5 suggested use as an anti-biofilm agent such as in the decontamination of medical devices.
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, University of Central Lancashire, Preston PR1 2HE, UK.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Dennison
authors.forename: Leslie H G
authors.initials: LH
authors.lastname: Morton
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: Shorrocks
authors.forename: Frederick
authors.initials: F
authors.lastname: Harris
authors.forename: David A
authors.initials: DA
authors.lastname: Phoenix
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000166:cytology* / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects*; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects*; D008055:Lipids / Q000737:chemistry; D010933:Plankton / Q000166:cytology / Q000187:drug effects; D011312:Pressure; D013499:Surface Properties / Q000187:drug effects; D013696:Temperature; D013816:Thermodynamics
chemical_list: D023181:Antimicrobial Cationic Peptides; D008055:Lipids; C535241:aurein 2.5 peptide, Litoria aurea