title: A draft genome sequence of Pseudomonas syringae pv. tomato T1 reveals a type III effector repertoire significantly divergent from that of Pseudomonas syringae pv. tomato DC3000.
abstract: Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those interactions governing pathogen-host specificity. Comparative genomics of closely related pathogens with different host specificity represents an excellent approach for identification of genes contributing to host-range determination. A draft genome sequence of Pseudomonas syringae pv. tomato T1, which is pathogenic on tomato but nonpathogenic on Arabidopsis thaliana, was obtained for this purpose and compared with the genome of the closely related A. thaliana and tomato model pathogen P. syringae pv. tomato DC3000. Although the overall genetic content of each of the two genomes appears to be highly similar, the repertoire of effectors was found to diverge significantly. Several P. syringae pv. tomato T1 effectors absent from strain DC3000 were confirmed to be translocated into plants, with the well-studied effector AvrRpt2 representing a likely candidate for host-range determination. However, the presence of avrRpt2 was not found sufficient to explain A. thaliana resistance to P. syringae pv. tomato T1, suggesting that other effectors and possibly type III secretion system-independent factors also play a role in this interaction.
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Nalvo F
authors.initials: NF
authors.lastname: Almeida
authors.forename: Shuangchun
authors.initials: S
authors.lastname: Yan
authors.forename: Magdalen
authors.initials: M
authors.lastname: Lindeberg
authors.forename: David J
authors.initials: DJ
authors.lastname: Studholme
authors.forename: David J
authors.initials: DJ
authors.lastname: Schneider
authors.forename: Bradford
authors.initials: B
authors.lastname: Condon
authors.forename: Haijie
authors.initials: H
authors.lastname: Liu
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Viana
authors.forename: Andrew
authors.initials: A
authors.lastname: Warren
authors.forename: Clive
authors.initials: C
authors.lastname: Evans
authors.forename: Eric
authors.initials: E
authors.lastname: Kemen
authors.forename: Dan
authors.initials: D
authors.lastname: Maclean
authors.forename: Aurelie
authors.initials: A
authors.lastname: Angot
authors.forename: Gregory B
authors.initials: GB
authors.lastname: Martin
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Jones
authors.forename: Alan
authors.initials: A
authors.lastname: Collmer
authors.forename: Joao C
authors.initials: JC
authors.lastname: Setubal
authors.forename: Boris A
authors.initials: BA
authors.lastname: Vinatzer
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods; D018551:Solanum lycopersicum / Q000254:growth & development / Q000382:microbiology; D008957:Models, Genetic; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial