Quantification of bovine casein fractions by direct chromatographic analysis of milk. Approaching the application to a real production context.
The ability to quantify the casein content by an exact and cost-effective approach represents an issue of crucial importance in the dairy industry as the natural variations in milk protein concentration can markedly affect the yield of the cheesemaking processes, thus causing a direct and significant economic impact on the producers. In this work, the separation and quantification of alpha(s1)-, alpha(s2)-, kappa- and beta-casein was carried out by direct RP-HPLC analysis of milk. The identification of each casein was established by electrospray ionization mass spectrometry. The data show that this method is able to effectively separate the bovine casein fractions, it provides simplified analytical conditions (with special regard to mobile phase composition and gradient profile) and faster separation while ensuring adequate precision to achieve reliable quantifications in milk samples from dairy production.
Bonizzi
Ivan
I
Laboratorio Qualità dei Prodotti, Istituto Sperimentale Italiano "Lazzaro Spallanzani", Via Einstein, località Cascina Codazza, 26900 Lodi, Italy. ivan.bonizzi@isils.it
Buffoni
Joanna Natalia
JN
Feligini
Maria
M
D000818:Animals; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D002614:Chelating Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D002364:Caseins; D002614:Chelating Agents