[RNA interference silencing excision repair cross-complementing 1 gene and affecting cisplatin sensitivity on lung cancer cell lines].
OBJECTIVE
To research the changes of cisplatin sensitivity by RNA interfering the excision repair cross-complementing (ERCC)1 gene in lung cancer cell lines.


METHODS
The small interference RNA (siRNA) targeting ERCC1 gene was designed and synthesized by transcription in vitro, and transfected to lung cancer cell line A549. The mRNA and protein of ERCC1 were evaluated by means of RT-PCR, Western blot and immunocytochemistry. The changes of cisplatin sensitivity after interference were examined by methyl thiazolyl tetrazolium (MTT) assay.


RESULTS
In A549 cell, the mRNA and protein levels of ERCC1 were dramatically decreased 24, 48 and 72 hours after transfection. The sensitivity to cisplatin of A549 cell line was increased by 3.07 times after disturbing the ERCC1 gene.


CONCLUSIONS
The sensitivity to cisplatin of lung cancer cell lines A549 could be enhanced by RNA interfering ERCC1 gene.
Shang
Xiao-bin
XB
Department of Esophageal Tumor, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. sxbin@eyou.com
Yu
Zhen-tao
ZT
Tang
Peng
P
Zhang
Xi-zeng
XZ
D000230:Adenocarcinoma / Q000188:drug therapy; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy; D034741:RNA, Small Interfering; D014162:Transfection
D000970:Antineoplastic Agents; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; C071447:ERCC1 protein, human; D004720:Endonucleases; D002945:Cisplatin