title: A facile method for the detection of aldehydes in oxidized lipids using solid-phase microextraction fiber and gas chromatograph equipped with a septum-free injector.
abstract: A facile method is described for the detection of volatile odor components from oxidized lipids. The method is based on headspace solid-phase microextraction (HS-SPME) of the compounds in the vial head space and the subsequent analysis of the volatiles by gas chromatography (GC) using a septum-free sample injector. The extraction was done using a 85 microm carboxen-poly(dimethylsiloxane) SPME fiber and the volatiles were desorbed onto a GC instrument with the septum-free injection port. The septum free injector made it possible to analyze the samples without using a conventional silicone rubber septum, that is easily damaged by the repetitive stubs of a relatively thick blunt needle into the septum and a long desorption time after injection. The method was standardized with authentic C(3)-C(10) aldehydes and was applied to the detection of aldehyde components from oxidized fish oil. Several volatiles, including propanal and hexanal, were clearly separated on a polar capillary column (60 m) with reproducible retention times. The present study demonstrates that the method using the GC septum-free injector and the HS-SPME fiber would be useful for the determination of volatile components derived from edible oils.
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hokkaido, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Arato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
authors.forename: Kazuo
authors.initials: K
authors.lastname: Miyashita
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Itabashi
mesh_terms: D000447:Aldehydes / Q000032:analysis*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D004041:Dietary Fats / Q000378:metabolism*; D050356:Lipid Metabolism; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D014835:Volatilization
chemical_list: D000447:Aldehydes; D004041:Dietary Fats