Allogeneic intra-BM-BMT plus adult thymus transplantation from same donor has benefits for long-term survival even after sublethal irradiation or low-dose BM cell injection.
We examined the effects of intra-BM-BMT (IBM-BMT) plus adult thymus transplantation (ATT) from the same donor after 5.5 Gy sublethal irradiation (SubLI) or low-dose (3 x 10(6)) BM cell injection (LDBMCI). With SubLI, BALB/c mice that had received 1 x 10(7) bone marrow cells by IBM-BMT plus ATT from B6 mice showed 73% donor chimerism, whereas those treated with IBM-BMT alone showed 45% chimerism. In the LDBMCI with 7Gy irradiation, IBM-BMT plus ATT resulted in a 90% survival rate with 90% chimerism, whereas IBM-BMT alone resulted in a 55% survival rate with 44% chimerism. Although the number of CD4 T cells was higher in IBM-BMT plus ATT than in IBM-BMT alone, the percentages of FoxP3+/CD4+ T cells and lymphocyte functions in the former were almost identical to those in the latter. When treated with IBM-BMT plus donor lymphocyte infusion (DLI), the mice showed a reduced survival time as a result of GVHD, with low numbers of FoxP3+CD4 T cells under either condition, although 100% chimerism was induced. These results suggest that IBM-BMT plus ATT is effective in reconstituting the recipients with donor-derived cells even after SubLI or LDBMCI.
Nishida
T
T
Department of Pathology, Kansai Medical University, Moriguchi, Osaka, Japan.
Hosaka
N
N
Takaki
T
T
Miyake
T
T
Cui
W
W
Inaba
M
M
Kinoshita
H
H
Matsuda
T
T
Ikehara
S
S
D000818:Animals; D001835:Body Weight; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000379:methods; D005260:Female; D006085:Graft Survival; D007421:Intestine, Small / Q000473:pathology; D008099:Liver / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D015996:Survival Rate; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology / Q000637:transplantation*; D018183:Transplantation Chimera / Q000276:immunology; D023001:Transplantation Tolerance / Q000276:immunology; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D014916:Whole-Body Irradiation