title: [Comparison between Neuropilin-1(+)T cells and CD4(+)CD25(+) regulatory T cells].
abstract: OBJECTIVE
  To analyze the relationship between Nrp-1(neuropilin-1) positive T cells (Nrp-1(+)T cells) and CD4(+)CD25(+) regulatory T cells (CD4(+)CD25(+)Treg) and compare their immunoregulatory effects.
  
  
  METHODS
  The expression of Nrp-1, CD4 and CD25 on the splenic T cells of BALB/c mice was detected by flow cytometry and Nrp-1(+)T cells and CD4(+)CD25(+)Treg were sorted. Then their effects of on NK cells killing B16-F10-luc-G5 melanoma cells were compared in vitro by bioluminescence imaging system.
  
  
  RESULTS
  The expression of Nrp-1 on CD4(+)CD25(+)Treg was (27.28+/-1.17)%, which was significantly higher than that (1.63+/-0.08)% on CD4(+)CD25(-) T cells(P<0.01). Both Nrp-1(+)T cells and CD4(+)CD25(+)Treg inhibited the effect of killing NK cells on B16-F10-luc-G5 melanoma cells in vitro. At 6, 24, 48 and 72 h, the number of tumor cells in Nrp-1(+)T cell group was higher than that in CD4(+)CD25(+)Treg group(984+/-15 vs 931+/-4, 1015+/-14 vs 983+/-8, 1261+/-21 vs 1201+/-18 and 1323+/-38 vs 1256+/-18, respectively). There was significant statistical difference between the two groups at each time point (P<0.01).
  
  
  CONCLUSIONS
  The proportion of CD4(+)CD25(+)Treg expressing Nrp-1 is high. Nrp-1(+)T cells show negtive immunoregulatory effect, which is more powerful than CD4(+)CD25(+)Treg. Nrp-1(+)T cells may be regarded as a new subgroup of Treg.
authors.affiliation: Organ Transplantation Center of PLA, the Second Affiliated Hospital of General Hospital of PLA, Beijing 100091, China.
authors.forename: Xiang-Ke
authors.initials: XK
authors.lastname: Pei
authors.forename: Ming
authors.initials: M
authors.lastname: Cai
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Zhou
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Xu
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Chen
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Shi
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism*; D002478:Cells, Cultured; D005434:Flow Cytometry; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D039942:Neuropilin-1 / Q000276:immunology* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit; D039942:Neuropilin-1