title: [Apoptosis induced by NNAMB, a novel polyamine conjugate, in human erythroleukemia K562 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the apoptosis-inducing effects of NNAMB, a novel polyamine conjugate, in erythroleukemia K562 cells and its molecular mechanism.
  
  
  METHODS
  Cell viability was assessed by MTT assay and trypan blue dye exclusion method. The cell morphology was observed by fluorescence microscopy. The cell cycle distribution, apoptosis and mitochondrial membrane potential were measured by flow cytometry. The expression of caspase-3, -8, -9, cytochrome c in the K562 cells was detected by Western blot.
  
  
  RESULTS
  NNAMB inhibited the proliferation of K562 cells. The cells treated with NNAMB showed a typical apoptotic morphology, Sub-G1 peak and loss of mitochondrial membrane potential. Western blot assay showed that NNAMB increased the expression of caspase-3, -9, cytochrome c but not caspase-8 in a dose-and time-dependent manner.
  
  
  CONCLUSIONS
  NNAMB induces apoptosis via mitochondrial pathway in K562 cells.
authors.affiliation: Department of Pharmacology, Henan University, Kaifeng 475001, China.
authors.forename: Song-Qiang
authors.initials: SQ
authors.lastname: Xie
authors.forename: Ying-Liang
authors.initials: YL
authors.lastname: Wu
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Liu
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Cheng
authors.forename: Min-Wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Ma
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Chao-Jie
authors.initials: CJ
authors.lastname: Wang
mesh_terms: D000873:Anthracenes / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D011073:Polyamines / Q000494:pharmacology*; D013095:Spermidine / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D000873:Anthracenes; C548263:NNAMB compound; D011073:Polyamines; D045304:Cytochromes c; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D013095:Spermidine