title: Determination of ultra-trace amount methyl-, phenyl- and inorganic mercury in environmental and biological samples by liquid chromatography with inductively coupled plasma mass spectrometry after cloud point extraction preconcentration.
abstract: The cloud point extraction (CPE) preconcentration of ultra-trace amount of mercury species prior to reverse-phase high performance liquid chromatography (HPLC) with inductively coupled plasma mass spectrometry (ICP-MS) detection was studied. Mercury species including methyl-, ethyl-, phenyl- and inorganic mercury were transformed into hydrophobic chelates by reaction with sodium diethyldithiocarbamate, and the hydrophobic chelates were extracted into a surfactant-rich phase of Triton X-114 upon heating in a water bath at 40 degrees C. Ethylmercury was found partially decomposed during the CPE process, and was not included in the developed method. Various experimental conditions affecting the CPE preconcentration, HPLC separation, and ICP-MS determination were optimized. Under the optimized conditions, detection limits of 13, 8 and 6 ng l(-1) (as Hg) were achieved for MeHg(+), PhHg(+) and Hg(2+), respectively. Seven determinations of a standard solution containing the three mercury species each at 0.5 ng ml(-1) level produced relative standard deviations of 5.3, 2.3 and 4.4% for MeHg(+), PhHg(+) and Hg(2+), respectively. The developed method was successfully applied for the determination of the three mercury species in environmental water samples and biological samples of human hair and ocean fish.
authors.affiliation: Department of Chemistry, Zhejiang University, Zijin'gang Campus, Hangzhou 310058, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.forename: Hengwu
authors.initials: H
authors.lastname: Chen
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Jin
authors.forename: Haiting
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D005035:Ethylmercury Compounds / Q000032:analysis*; D005399:Fishes; D005506:Food Contamination; D006197:Hair; D008628:Mercury / Q000032:analysis*; D008767:Methylmercury Compounds / Q000032:analysis*; D017830:Octoxynol; D010663:Phenylmercury Compounds / Q000032:analysis*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013696:Temperature
chemical_list: D003902:Detergents; D005035:Ethylmercury Compounds; D008767:Methylmercury Compounds; D010663:Phenylmercury Compounds; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D008628:Mercury