title: Malnourished mice display an impaired hematologic response to granulocyte colony-stimulating factor administration.
abstract: The aim of this study was to determine if protein-energy malnutrition could affect the hematologic response to granulocyte colony-stimulating factor (G-CSF). Swiss mice were fed a low-protein diet containing 4% protein, whereas control mice were fed a 20% protein-containing diet. After the malnourished group lost 20% of their original body weight, the mice were subdivided in 2 treatment groups, and hematopoietic parameters were studied. Mice were injected with either 8 microg/kg per day of G-CSF or saline twice daily for 4 days. Malnourished mice developed anemia with reticulopenia and leukopenia with depletion of granulocytes and lymphocytes. Both malnourished and control mice treated with G-CSF showed a significant increase in neutrophils; however, in the control group, this increase was more pronounced compared to the malnourished group (4.5-fold and 3.4-fold, respectively). Granulocyte colony-stimulating factor administration increased bone marrow blastic (P < .001) and granulocytic (P < .01) compartments in the controls but had no significant effect on these hematopoietic compartments in the malnourished animals (P = .08 and P = .62, respectively). We report that malnourished mice display an impaired response to G-CSF, which contributes to the decreased production of leukocytes in protein-energy malnutrition.
authors.affiliation: Laboratory of Cellular Physiology, Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, 05508-900 Butantã, São Paulo, Brasil.
authors.forename: Marco Aurélio Ramirez
authors.initials: MA
authors.lastname: Vinolo
authors.forename: Amanda Rabello
authors.initials: AR
authors.lastname: Crisma
authors.forename: Karina
authors.initials: K
authors.lastname: Nakajima
authors.forename: Marcelo Macedo
authors.initials: MM
authors.lastname: Rogero
authors.forename: Ricardo Ambrósio
authors.initials: RA
authors.lastname: Fock
authors.forename: Primavera
authors.initials: P
authors.lastname: Borelli
mesh_terms: D000740:Anemia / Q000209:etiology*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001854:Bone Marrow Cells / Q000187:drug effects; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007958:Leukocyte Count; D007970:Leukopenia / Q000503:physiopathology; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D011502:Protein-Energy Malnutrition / Q000097:blood* / Q000150:complications; D011994:Recombinant Proteins; D013154:Spleen / Q000187:drug effects
chemical_list: D015415:Biomarkers; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor