title: [Application of orthogonal design in tissue culture of Dioscorea opposite].
abstract: OBJECTIVE
  To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose.
  
  
  METHODS
  stems were selected as explants. The effects of three kinds of factors were studied by orthogonal design method including sucrose, 6-BA, NAA on the buds and microtuber induction. The data were analyzed with range analysis and vadance analysis. RESULT AND CONDUSION: The optimal media to induce many buds from stems were MS + 6-BA 1 mg x L(-1) + NAA 1 mg x L(-1) + sucrose 3%, the effect of the three factors was in sequence of sucrose >6-BA > NAA. The optimal media to induce microtuber from stems were MS+6-BA 1.5 mg x V1 +NAA 1.5 mg x L(-1) + sucrose 5%, the effect of the three factors was in sequence of sucrose >6-BA > NAA.
authors.affiliation: Department of Biology, Huaibei Coal Industry Teachers' College, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China. xuejp2000@yahoo.com.cn
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Xiao-quan
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Zhang
authors.forename: Yue-qin
authors.initials: YQ
authors.lastname: Huang
mesh_terms: D003470:Culture Media; D027742:Dioscorea / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D013395:Sucrose