[Cloning and sequencing of the papA gene from uropathogenic Escherichia coli 4030 strain].
OBJECTIVE
Cloning and sequencing of the papA gene from uropathogenic Escherichia coli 4030 strain to investigate the differences of the sequences of the papA of UPEC4030 strain and the ones of related genes, in order to make whether or not it was a new genotype.


METHODS
Cloning and sequencing methods were used to analyze the sequence of the papA of UPEC4030 strain in comparison with related sequences.


RESULTS
The sequence analysis of papA revealed a 722 bp gene and encode 192 amino acid polypeptide. The overall homology of the papA genes between UPEC4030 and the standard strains of ten F types were 36.11%-77.95% and 22.20%-78.34% at nucleotide and deduced amino acid levels. The homology between the sequence of the reverse primers and the corresponding sequence of UPEC4030 papA was 10%-66.67%. The results confirmed that UPEC4030 strain contained a novel papA variant.


CONCLUSIONS
UPEC4030 strain could contain an unknown papA variant or the novel genotype. The pathogenic mechanism and epidemiology related need to be further studied.
Wu
Qinggang
Q
Wuxi Center for Disease Control and Prevention, Wuxi 214023, China.
Zhang
Jingping
J
Zhao
Chuncheng
C
Zhu
Jianguo
J
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D014552:Urinary Tract Infections / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D018830:Adhesins, Escherichia coli; C078802:AtpA protein, E coli; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins