Mirror image DNA nanostructures for chiral supramolecular assemblies.
L-DNA, the mirror image of natural D-DNA, can be readily self-assembled into designer discrete or periodic nanostructures. The assembly products are characterized by polyacrylamide gel electrophoresis, circular dichroism spectrum, atomic force microscope, and fluorescence microscope. We found that the use of enantiomer DNA as building material leads to the formation of DNA supramolecules with opposite chirality. Therefore, the L-DNA self-assembly is a substantial complement to the structural DNA nanotechnology. Moreover, the L-DNA architectures feature superior nuclease resistance thus are appealing for in vivo medical applications.
Lin
Chenxiang
C
Department of Chemistry and Biochemistry and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
Ke
Yonggang
Y
Li
Zhe
Z
Wang
James H
JH
Liu
Yan
Y
Yan
Hao
H
D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D010316:Particle Size; D013237:Stereoisomerism; D013499:Surface Properties
D046911:Macromolecular Substances; D004247:DNA