title: Wave expansion of CD34+ progenitor cells in the spleen in rodent malaria.
abstract: Defense against malaria depends upon amplification of the spleen structure and function for the clearance of parasitized red blood cells (pRBC). We studied the distribution and amount of CD34(+) cells in the spleens of mice infected with rodent malaria. We sought to identify these cells in the spleen and determine their relationship to infection. C57BL/6J mice were infected with self-resolving, Plasmodium chabaudi CR, or one of the lethal rodent malaria strains, P. chabaudi AJ and P. berghei ANKA. We then recorded parasitemia, mortality, and the presence of CD34(+) cells in spleen, as determined by immunohistochemistry and flow cytometry. In the non-lethal strain, the spleen structure was maintained during amplification, but disrupted in lethal models. The abundance of CD34(+) cells increased in the red pulp on the 4th and 6th days p.i. in all models, and subsided on the 8th day p.i. Faint CD34(+) staining on the 8th day p.i., was probably due to differentiation of committed cell lineages. In this work, increase of spleen CD34(+) cells did not correlate with infection control.
authors.affiliation: Instituto de Ciências Biomédicas da Universidade de São Paulo Professor Lineu Prestes Avenue, Cidade Universitária, São Paulo, SP 05508-900, Brazil.
authors.forename: Felipe Pessoa de Melo
authors.initials: FP
authors.lastname: Hermida
authors.forename: Daniel Perez
authors.initials: DP
authors.lastname: Vieira
authors.forename: Elaine Raniero
authors.initials: ER
authors.lastname: Fernandes
authors.forename: Heitor Franco
authors.initials: HF
authors.lastname: de Andrade
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018512:Parasitemia / Q000276:immunology; D010962:Plasmodium berghei / Q000276:immunology*; D016788:Plasmodium chabaudi / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
chemical_list: D018952:Antigens, CD34