Towards structural elucidation of eukaryotic photosystem II: Purification, crystallization and preliminary X-ray diffraction analysis of photosystem II from a red alga.
Crystal structure of photosystem II (PSII) has been reported from prokaryotic cyanobacteria but not from any eukaryotes. In the present study, we improved the purification procedure of PSII dimers from an acidophilic, thermophilic red alga Cyanidium caldarium, and crystallized them in two forms under different crystallization conditions. One had a space group of P222(1) with unit cell constants of a=146.8 A, b=176.9 A, and c=353.7 A, and the other one had a space group of P2(1)2(1)2(1) with unit cell constants of a=209.2 A, b=237.5 A, and c=299.8 A. The unit cell constants of both crystals and the space group of the first-type crystals are different from those of cyanobacterial crystals, which may reflect the structural differences between the red algal and cyanobacterial PSII, as the former contains a fourth extrinsic protein of 20 kDa. X-ray diffraction data were collected and processed to a 3.8 A resolution with the first type crystal. For the second type crystal, a post-crystallization treatment of dehydration was employed to improve the resolution, resulting in a diffraction data of 3.5 A resolution. Analysis of this type of crystal revealed that there are 2 PSII dimers in each asymmetric unit, giving rise to 16 PSII monomers in each unit cell, which contrasts to 4 dimers per unit cell in cyanobacterial crystals. The molecular packing of PSII within the unit cell was constructed with the molecular replacement method and compared with that of the cyanobacterial crystals.
Adachi
Hideyuki
H
Division of Biosciences, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Umena
Yasufumi
Y
Enami
Isao
I
Henmi
Takahiro
T
Kamiya
Nobuo
N
Shen
Jian-Ren
JR
D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D008958:Models, Molecular; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011487:Protein Conformation; D000461:Rhodophyta / Q000378:metabolism*
D045332:Photosystem II Protein Complex; D010100:Oxygen