A facile method for the detection of aldehydes in oxidized lipids using solid-phase microextraction fiber and gas chromatograph equipped with a septum-free injector.
A facile method is described for the detection of volatile odor components from oxidized lipids. The method is based on headspace solid-phase microextraction (HS-SPME) of the compounds in the vial head space and the subsequent analysis of the volatiles by gas chromatography (GC) using a septum-free sample injector. The extraction was done using a 85 microm carboxen-poly(dimethylsiloxane) SPME fiber and the volatiles were desorbed onto a GC instrument with the septum-free injection port. The septum free injector made it possible to analyze the samples without using a conventional silicone rubber septum, that is easily damaged by the repetitive stubs of a relatively thick blunt needle into the septum and a long desorption time after injection. The method was standardized with authentic C(3)-C(10) aldehydes and was applied to the detection of aldehyde components from oxidized fish oil. Several volatiles, including propanal and hexanal, were clearly separated on a polar capillary column (60 m) with reproducible retention times. The present study demonstrates that the method using the GC septum-free injector and the HS-SPME fiber would be useful for the determination of volatile components derived from edible oils.
Arato
Shingo
S
Faculty of Fisheries Sciences, Hokkaido University, Hokkaido, Japan.
Ito
Hiroshi
H
Miyashita
Kazuo
K
Hayakawa
Kazuichi
K
Itabashi
Yutaka
Y
D000447:Aldehydes / Q000032:analysis*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D004041:Dietary Fats / Q000378:metabolism*; D050356:Lipid Metabolism; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D014835:Volatilization
D000447:Aldehydes; D004041:Dietary Fats