Expression of selenoprotein-coding genes SEPP1, SEP15 and hGPX1 in non-small cell lung cancer.
Aim of the study was to investigate the mRNA expression level of selenoprotein P (SEPP1), 15-kDa selenoprotein (SEP15) and glutathione peroxidase 1 (hGPX1) in paired malignant and non-malignant tissue. To achieve this goal, the quantitative real-time PCR technique was utilized in paired tissue samples from 33 non-small cell lung cancer (NSCLC) patients. Simultaneously, the activity of glutathione peroxidases (GPX) and the level of thiobarbituric acid-reactive species (TBARS) in paired tissue specimens and the blood plasma selenium level was measured. We found significant down-regulation of SEPP1 expression level in tumorous lung tissue (2.732-fold; p<0.001). The expression of hGPX1 and SEP15 in tumorous tissue remained unchanged compared to healthy tissue. The level of TBARS in malignant tissue was significantly increased (p<0.005) and negatively correlated with SEPP1 expression level (R(S)=-0.3238; p<0.05). The activity of GPX in malignant tissue was significantly increased compared to the non-malignant one (p<0.005) and negatively correlated with the expression level of SEPP1. It seems possible, that the down-regulation of SEPP1 expression may lead to an increased oxidative stress possibly resulting in lung carcinogenesis. Increased activity of GPX in tumorous lung tissue seems to be a feedback mechanism.
Gresner
Peter
P
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8, Sw. Teresy St., Lodz 91-348, Poland. pgresner@yahoo.com
Gromadzinska
Jolanta
J
Jablonska
Ewa
E
Kaczmarski
Jacek
J
Wasowicz
Wojciech
W
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005979:Glutathione Peroxidase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012643:Selenium / Q000097:blood; D051149:Selenoprotein P / Q000096:biosynthesis* / Q000235:genetics; D051140:Selenoproteins / Q000096:biosynthesis* / Q000235:genetics; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
D012333:RNA, Messenger; C495238:SELENOF protein, human; D051149:Selenoprotein P; D051140:Selenoproteins; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human