title: The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration.
abstract: Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
authors.affiliation: Institute for Animal Health, Compton, Newbury, Berkshire, UK.
authors.forename: Brenda V
authors.initials: BV
authors.lastname: Jones
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Hennion
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D017934:Coronavirus / Q000254:growth & development / Q000302:isolation & purification; D001351:Infectious bronchitis virus / Q000254:growth & development / Q000302:isolation & purification; D009924:Organ Culture Techniques / Q000379:methods; D014132:Trachea / Q000821:virology*