[Expression of angiopoietin-1,2 and its relationship with angiogenesis in laryngeal squamous cell carcinoma].
OBJECTIVE
To investigate the expression of Angiopoietin-1,2 in laryngeal squamous cell carcinoma (LSCC) and the relationship between Angiopoietin-1,2 expression and clinicopathologic parameters and microvessel density (MVD) marked by CD105, and also evaluate the significance of co-expression of Ang-2 and vascular endothelial cell growth factor (VEGF) in tumor angiogenesis.


METHODS
The expression of Ang-1,2 and VEGF in samples of tumor, para cancer and normal mucosa were detected by immunohistochemical method.


RESULTS
The expression of Angiopoietin-1,2 were identified in LSCC, para cancer tissue and normal mucosa. The VEGF expression was only existed in LSCC. The expression of Ang-1,2 were significantly higher in LSCC than in para cancer tissue (P < 0.05) and normal mucosa (P < 0.01). In the clinicopathologic parameters, only the expression of Ang-2 was closely correlated with clinical stages and MVD (all P < 0.05). There was no significant correlation between the expression of Ang-1,2 and tumor differentiation degree (all P > 0.05). When the expression of Ang-2 and VEGF were both positive, the mean value of MVD was higher than others (P < 0.05).


CONCLUSIONS
These results suggest that overexpression of Ang-1,2 may play a very important role in the development of LSCC and are closely correlated with angiogenesis. Ang-2 promote angiogenesis when interacting with VEGF.
Xu
Guang
G
Department of Otolaryngology, First Hospital of Zhengzhou University, Zhengzhou 450052, China.
Lou
Weihua
W
Sun
Zhanwei
Z
Zhang
Peng
P
D000368:Aged; D042683:Angiopoietin-1 / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D042683:Angiopoietin-1; D042702:Angiopoietin-2; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A