Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors.
OBJECTIVE
To assess whether different genomic cell-free DNAs are equally abundant in the plasma of individual donors, and any relationship between DNA methylation and representation in plasma.


METHODS
The concentrations of DNA in plasma were determined by real-time PCR.


RESULTS
Different DNA sequences were not equally represented. The relative abundances were similar in different donors.


CONCLUSIONS
Different DNA sequences are not equally abundant in plasma, with no relationship between DNA methylation and abundance.
Puszyk
William M
WM
Department of Biological Sciences, University of Warwick, Coventry, UK.
Crea
Francesco
F
Old
Robert W
RW
D001483:Base Sequence; D004247:DNA / Q000097:blood* / Q000737:chemistry*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004247:DNA