title: A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region.
abstract: The analysis of X-STR polymorphisms has received the attention of several researchers, mainly due to its applicability to the investigation of complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in different populations of the world. The present work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. The loci investigated include DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011, which together afford a power of discrimination in the order of 0.999999. In addition, this work presents the genotyping results obtained for a sample of 324 individuals (182 males and 142 females) from the admixed population of Belém, Pará, located in the Brazilian Amazon Region.
authors.affiliation: Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil.
authors.forename: Elzemar Martins
authors.initials: EM
authors.lastname: Ribeiro Rodrigues
authors.forename: Fabio Pereira das Neves
authors.initials: FP
authors.lastname: Leite
authors.forename: Mara Helena
authors.initials: MH
authors.lastname: Hutz
authors.forename: Teresinha de Jesus Brabo Ferreira
authors.initials: Tde J
authors.lastname: Palha
authors.forename: Andrea Kely Campos
authors.initials: AK
authors.lastname: Ribeiro dos Santos
authors.forename: Sidney Emanuel Batista
authors.initials: SE
authors.lastname: dos Santos
mesh_terms: D001938:Brazil; D041321:Chromosomes, Human, X; D005260:Female; D053803:Forensic Genetics; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction; D045483:Rivers
chemical_list: D005819:Genetic Markers