Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip.
The present paper described a rapid and ultrasensitive detection method using a microfluidic chip for analyzing 7-aminoclonazepam (7-ACZP) residues in human urine. A microfluidic chip-based immunoassay with laser-induced fluorescence (LIF) detection based on the water-soluble denatured bovine serum albumin (dBSA)-coated CdTe quantum dots (QDs) was prepared for the ultrasensitive detection of 7-ACZP. The whole procedure including the chip and the control software was designed and constructed in our own laboratory. The detection of 7-ACZP could be completed within 5 min. The results demonstrated that under the optima conditions, 7-ACZP residues could be detected with a precision of 5% relative standard deviation (RSD), and the linear range and the limit of detection (LOD) for 7-ACZP were 1.1-60.1 and 0.021 ngmL(-1), respectively. This method was compared with ELISA and showed a good correlation. This microfluidic chip with LIF detection was applied to the determination of 7-ACZP residues in positive human urine samples, and the results were confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC/MS/MS). This ultrasensitive detection technique was proved to be practical for clinical use.
Chen
Wei
W
School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, China.
Peng
Chifang
C
Jin
Zhengyue
Z
Qiao
Ruirui
R
Wang
Wuyang
W
Zhu
Shuifang
S
Wang
Libing
L
Jin
Qinhui
Q
Xu
Chuanlai
C
D000927:Anticonvulsants / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation; D019187:Cadmium Compounds / Q000737:chemistry*; D002998:Clonazepam / Q000031:analogs & derivatives* / Q000652:urine; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008832:Microchemistry / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013691:Tellurium / Q000737:chemistry*; D016482:Urinalysis / Q000295:instrumentation*
D000927:Anticonvulsants; D019187:Cadmium Compounds; C020860:7-aminoclonazepam; D002998:Clonazepam; D013691:Tellurium; C028337:cadmium telluride