title: New alleles and mutational events at 14 STR loci from different German populations.
abstract: The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.
authors.affiliation: Biotype AG, Moritzburger Weg 67 D, 01109 Dresden, Germany. d.becker@biotype.de
authors.forename: Dorit
authors.initials: D
authors.lastname: Becker
authors.forename: Klaus
authors.initials: K
authors.lastname: Bender
authors.forename: Jeanett
authors.initials: J
authors.lastname: Edelmann
authors.forename: Frank
authors.initials: F
authors.lastname: Götz
authors.forename: Lotte
authors.initials: L
authors.lastname: Henke
authors.forename: Sandra
authors.initials: S
authors.lastname: Hering
authors.forename: Carsten
authors.initials: C
authors.lastname: Hohoff
authors.forename: Karolin
authors.initials: K
authors.lastname: Hoppe
authors.forename: Michael
authors.initials: M
authors.lastname: Klintschar
authors.forename: Matthias
authors.initials: M
authors.lastname: Muche
authors.forename: Burkhard
authors.initials: B
authors.lastname: Rolf
authors.forename: Reinhard
authors.initials: R
authors.lastname: Szibor
authors.forename: Volker
authors.initials: V
authors.lastname: Weirich
authors.forename: Martin
authors.initials: M
authors.lastname: Jung
authors.forename: Werner
authors.initials: W
authors.lastname: Brabetz
mesh_terms: D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D053803:Forensic Genetics; D005787:Gene Frequency; D005828:Genetics, Population; D005858:Germany; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009154:Mutation
chemical_list: D017931:DNA Primers; D004247:DNA