Plasma membrane microdomains regulate turnover of transport proteins in yeast.
In this study, we investigate whether the stable segregation of proteins and lipids within the yeast plasma membrane serves a particular biological function. We show that 21 proteins cluster within or associate with the ergosterol-rich membrane compartment of Can1 (MCC). However, proteins of the endocytic machinery are excluded from MCC. In a screen, we identified 28 genes affecting MCC appearance and found that genes involved in lipid biosynthesis and vesicle transport are significantly overrepresented. Deletion of Pil1, a component of eisosomes, or of Nce102, an integral membrane protein of MCC, results in the dissipation of all MCC markers. These deletion mutants also show accelerated endocytosis of MCC-resident permeases Can1 and Fur4. Our data suggest that release from MCC makes these proteins accessible to the endocytic machinery. Addition of arginine to wild-type cells leads to a similar redistribution and increased turnover of Can1. Thus, MCC represents a protective area within the plasma membrane to control turnover of transport proteins.
Grossmann
Guido
G
Institute of Cell Biology and Plant Physiology, University of Regensburg, 93053 Regensburg, Germany.
Malinsky
Jan
J
Stahlschmidt
Wiebke
W
Loibl
Martin
M
Weig-Meckl
Ina
I
Frommer
Wolf B
WB
Opekarová
Miroslava
M
Tanner
Widmar
W
D026923:Amino Acid Transport Systems, Basic / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D020043:Genes, Essential; D016681:Genome, Fungal / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D017830:Octoxynol / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D026923:Amino Acid Transport Systems, Basic; D015415:Biomarkers; C502212:CAN1 protein, S cerevisiae; D002352:Carrier Proteins; C525535:NCE102 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D017830:Octoxynol