Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequential system.
OBJECTIVE
To compare embryologic and clinical outcomes in terms of preimplantation development, implantation, pregnancy rates, and secretome profile of implanted blastocysts from the preimplantation genetic diagnosis program grown in sequential versus endometrial epithelial cell (EEC) coculture system.


METHODS
Retrospective clinical study and prospective experimental study.


METHODS
In vitro fertilization clinical unit and university research laboratory.


METHODS
Blastomere biopsy, embryo culture, blastocyst transfer, and protein analysis of the media conditioned from implanted embryos obtained from coculture and sequential systems.


METHODS
Clinical study: blastocyst, implantation, and gestation rates in own and donated oocytes. Experimental study: differential protein analysis of implanted embryos grown in coculture system versus sequential system.


RESULTS
Of the 12,377 embryos analyzed, the blastocyst rates were 56.0% versus 45.9% in the coculture versus the sequential system, respectively, with own oocytes. With ovum donation, the rates were 70.5% versus 56.4%, respectively. Reproductive outcomes in terms of pregnancy rates (39.1% vs. 27.5%) and implantation rates (33.3% vs. 20.9%,) were statistically higher in EEC coculture versus sequential media. Furthermore, the protein profile of the EEC coculture versus the sequential system was obtained. Interleukin-6 (IL-6) was the most secreted protein by the EEC culture. Further ELISA experiments showed that the IL-6 present in the sequential medium diminished in implanted blastocysts.


CONCLUSIONS
The coculture system favors blastocyst development and implantation rates, given the contribution of the factors secreted by endometrial epithelial cells, such as IL-6.
Dominguez
Francisco
F
Fundación IVI, Instituto Universitario IVI, University of Valencia, C/Guadassuar 1 bajo, 46015 Valencia, Spain. fdominguez@ivi.es
Gadea
Blanca
B
Mercader
Amparo
A
Esteban
Francisco J
FJ
Pellicer
Antonio
A
Simón
Carlos
C
D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D018920:Coculture Techniques; D019295:Computational Biology; D017077:Culture Media, Conditioned / Q000378:metabolism; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D018587:Oocyte Donation; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D040081:Protein Array Analysis; D012189:Retrospective Studies; D056826:Single Embryo Transfer
D015415:Biomarkers; D017077:Culture Media, Conditioned; C508600:IL6 protein, human; D015850:Interleukin-6