title: TatABC overexpression improves Corynebacterium glutamicum Tat-dependent protein secretion.
abstract: The twin-arginine translocation (Tat) pathway in Corynebacterium glutamicum has been described previously. The minimal functional Tat system in C. glutamicum required TatA and TatC but did not require TatB, although this component was required for maximal efficiency of Tat-dependent secretion. We previously demonstrated that Chryseobacterium proteolyticum pro-protein glutaminase (pro-PG) and Streptomyces mobaraensis pro-transglutaminase (pro-TG) could be secreted via the Tat pathway in C. glutamicum. Here we report that the amounts of pro-PG secreted were more than threefold larger when TatC or TatAC was overexpressed, and there was a further threefold increase when TatABC was overexpressed. These results show that the amount of TatC protein is the first bottleneck and the amount of TatB protein is the second bottleneck in Tat-dependent protein secretion in C. glutamicum. In addition, the amount of pro-TG that accumulated via the Tat pathway when TatABC was overexpressed with the TorA signal peptide in C. glutamicum was larger than the amount that accumulated via the Sec pathway. We concluded that TatABC overexpression improves Tat-dependent pro-PG and pro-TG secretion in C. glutamicum.
authors.affiliation: Institute of Life Sciences, Ajinomoto Co., Inc., Kawasaki, Japan. yoshimi_kikuchi@ajinomoto.com
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itaya
authors.forename: Masayo
authors.initials: M
authors.lastname: Date
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsui
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Wu
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D045247:Chryseobacterium / Q000201:enzymology / Q000235:genetics; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D018628:Gene Dosage; D015870:Gene Expression; D026901:Membrane Transport Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D011503:Transglutaminases; D000581:Amidohydrolases; C022331:peptidyl-glutaminase