title: Optimization of bacitracin production by Bacillus licheniformis B5.
abstract: Production of the polypeptide antibiotic bacitracin by a newly isolated Bacillus licheniformis B5 strain, using a laboratory medium was optimized. A medium of the following conditions was obtained: 1.5% glycerol as carbon source, 0.05% glutamic acid as nitrogen source, 1.43% inorganic phosphate, 0.0025% magnesium sulfate, with an initial pH of 7.5. Culture was incubated at 37 degrees C for 48 h. The bacitracin productivity was about 174.2 units mL(-1). Bacitracin antibiotic was also produced using immobilized cells of the B5 isolate in different immobilization supports (7.5% polyacrylamide, 4% sodium alginate beads and 2% agar). It was found that the highest bacitracin production rate (192 units mL(-1)) was obtained when using 7.5% polyacrylamide gel as an immobilization material.
authors.affiliation: Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq.
authors.forename: Houria Ouled
authors.initials: HO
authors.lastname: Haddar
authors.forename: Ghazi Munim
authors.initials: GM
authors.lastname: Aziz
authors.forename: Majed Hussein
authors.initials: MH
authors.lastname: Al-Gelawi
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001414:Bacitracin / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D018698:Glutamic Acid; D005990:Glycerol; D008278:Magnesium Sulfate; D010710:Phosphates
chemical_list: D010710:Phosphates; D001414:Bacitracin; D018698:Glutamic Acid; D008278:Magnesium Sulfate; D005990:Glycerol