Changes in regulatory microRNA expression in myocardium of heart failure patients on left ventricular assist device support.
BACKGROUND
Left ventricular assist device support in heart failure patients leads to changes in mRNA and protein expression in the myocardium.


METHODS
MicroRNA's are important regulators of various cellular processes, so changes in their expression were tested by Q-PCR methods.


RESULTS
LVAD support (independently of the duration) leads to a decrease of the expression of miR-1, miR-133a and miR-133b in DCM patients but to an increase in expression in IHD patients. The expression of miR's pre- and post-LVAD in heart failure patients was low compared to the level of miR's in control myocardial tissue.


CONCLUSIONS
Alterations in miRs expression might also be important during repair processes in IHD patients.
Schipper
Marguérite E I
ME
Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands.
van Kuik
Joyce
J
de Jonge
Nicholas
N
Dullens
Hub F J
HF
de Weger
Roel A
RA
D000328:Adult; D001344:Autopsy; D005260:Female; D006321:Heart / Q000503:physiopathology*; D006333:Heart Failure / Q000235:genetics* / Q000601:surgery*; D016027:Heart Transplantation; D006353:Heart-Assist Devices; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014019:Tissue Donors; D018487:Ventricular Dysfunction, Left / Q000235:genetics / Q000503:physiopathology / Q000601:surgery
D035683:MicroRNAs; D012313:RNA