The autophagic induction in Helicobacter pylori-infected macrophage.
Helicobacter pylori has developed several mechanisms to evade the intracellular killing after phagocytosis. In this study, we reported that some Taiwanese clinical isolated H. pylori can multiply in human monocytic cells, such as THP-1 or U937 cells, but not in murine macrophage Raw264.7 cells. After internalization, there was a 5- to 10-fold increment of re-cultivable H. pylori from the infected THP-1 cells at 12 hrs post infection. The dividing H. pylori was found in a double-layer vesicle, which is characteristic of autophagosome. The formation of autophagosomes is associated with the multiplication of H. pylori in THP-1 cells. Its modulation with rapamycin or 3-MA affects the level of H. pylori replication. Furthermore, the VacA or CagA mutants of H. pylori have lower levels of multiplication in macrophages. We conclude that H. pylori infection induces autophagosome formation, and these autophagic vesicles were adapted for the multiplication of H. pylori in the host.
Wang
Ya-Hui
YH
Institute of Basic Medical Sciences, Department of Medical Technology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Wu
Jiunn-Jong
JJ
Lei
Huan-Yao
HY
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000382:microbiology / Q000648:ultrastructure; D009154:Mutation / Q000235:genetics; D020123:Sirolimus / Q000494:pharmacology; D014617:Vacuoles / Q000187:drug effects / Q000382:microbiology / Q000648:ultrastructure
D001426:Bacterial Proteins; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C025946:3-methyladenine; D000225:Adenine; D020123:Sirolimus