title: Genotyping of Scedosporium species: a review of molecular approaches.
abstract: Scedosporium species are increasingly encountered opportunistic fungal pathogens not only in immunocompromised patients but are also significant primary pathogens in immunocompetent individuals. The environmental reservoir of these fungi is uncertain and the epidemiology and mode of transmission are not well-defined. Conventional phenotypic methods are of limited use for epidemiological purposes since they are insensitive and inadequately discriminatory. Molecular techniques not only enable accurate phylogenetic delineation of species but also provide the means for rapid, reliable genotyping of strains for epidemiological and population genetic studies. This review discusses the methods that have been applied for genotyping of these increasingly important pathogens.
authors.affiliation: Molecular Mycology Research Laboratory, University of Sydney, Western Clinical School at Westmead Hospital, Westmead Millennium Institute, Sydney, Australia.
authors.forename: Azian
authors.initials: A
authors.lastname: Harun
authors.forename: Haybrig
authors.initials: H
authors.lastname: Perdomo
authors.forename: Felix
authors.initials: F
authors.lastname: Gilgado
authors.forename: Sharon C A
authors.initials: SC
authors.lastname: Chen
authors.forename: Josep
authors.initials: J
authors.lastname: Cano
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
authors.forename: Wieland
authors.initials: W
authors.lastname: Meyer
mesh_terms: D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods; D009181:Mycoses / Q000382:microbiology; D021681:Scedosporium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification