title: Cryopreservation induces temporal DNA methylation epigenetic changes and differential transcriptional activity in Ribes germplasm.
abstract: The physiological and molecular mechanisms associated with acclimation and survival have been examined in four Ribes genotypes displaying differential cryotolerance. Changes in DNA methylation, nucleic acid and nucleoside composition were determined during acclimation and recovery of in vitro shoot-meristems from cryopreservation. DNA methylation was induced in the tolerant genotype, while demethylation was evident in sensitive genotypes. This response initially occurred during sucrose simulated acclimation, with progressive changes as shoots recovered from successive stages of the encapsulation-dehydration protocol. These methylation patterns existed in the initial vegetative cycle but regressed to control values following subculture, indicating the changes in DNA methylation to be a reversible epigenetic mechanism. RNA levels indicating transcriptional activity during the acclimation of nodal tissue are inversely linked to methylation changes, where activity appears to be up-regulated in the cryosensitive genotypes. Conversely, cryopreserved shoots show increased levels of both RNA and DNA methylation in the cryotolerant genotypes. Other nucleosides show post-transcriptional activity corresponds with tolerance during acclimation and cryopreservation. These observations connect physiological attributes to differential molecular changes in Ribes, the implications of which are discussed in relation to cryopreservation-induced apoptosis and genetic stability.
authors.affiliation: Plant Conservation Group, School of Contemporary Science, University of Abertay Dundee, Kydd Building, Bell St, Dundee, DD1 1HG, UK. jjohnston@hortresearch.co.nz
authors.forename: Jason W
authors.initials: JW
authors.lastname: Johnston
authors.forename: Erica E
authors.initials: EE
authors.lastname: Benson
authors.forename: Keith
authors.initials: K
authors.lastname: Harding
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D019175:DNA Methylation; D018744:DNA, Plant / Q000032:analysis; D044127:Epigenesis, Genetic; D018520:Plant Shoots; D018749:RNA, Plant / Q000032:analysis; D031965:Ribes / Q000235:genetics*; D012639:Seeds; D015533:Transcriptional Activation
chemical_list: D018744:DNA, Plant; D018749:RNA, Plant