title: Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo Grande, MS, Brazil.
abstract: We analyzed fecal samples from hospitalized children up to three years of age with acute gastroenteritis at Campo Grande, Mato Grosso do Sul, Brazil, from May 2000-January 2004. Astrovirus and calicivirus were detected by Reverse Transcription-Polymerase Chain Reaction and adenovirus was detected using the Rotavirus and Adenovirus combined immunoenzyme assay. Astrovirus, adenovirus and calicivirus were detected at rates of 3.1%, 3.6% and 7.6%, respectively. These results re-emphasize the need for the establishment of regional vigilance systems to evaluate the impact of enteric viruses on viral gastroenteritis.
authors.affiliation: Departamento de Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil. msaa@nin.ufms.br
authors.forename: Marcia Sueli Assis
authors.initials: MS
authors.lastname: Andreasi
authors.forename: Divina das Dores de Paula
authors.initials: Dd
authors.lastname: Cardoso
authors.forename: Sonia Maria
authors.initials: SM
authors.lastname: Fernandes
authors.forename: Ines Aparecida
authors.initials: IA
authors.lastname: Tozetti
authors.forename: Ana Maria Tavares
authors.initials: AM
authors.lastname: Borges
authors.forename: Fabíola Souza
authors.initials: FS
authors.lastname: Fiaccadori
authors.forename: Rodrigo Alessandro Tôgo
authors.initials: RA
authors.lastname: Santos
authors.forename: Menira
authors.initials: M
authors.lastname: Souza
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000453:epidemiology*; D000260:Adenoviruses, Human / Q000302:isolation & purification; D019350:Astroviridae Infections / Q000175:diagnosis / Q000453:epidemiology*; D001938:Brazil / Q000453:epidemiology; D002139:Caliciviridae / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology*; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D001257:Mamastrovirus / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction