Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction.
Cunningham
Miles G
MG
McLean Hospital, Harvard Medical School, mcunningham@mclean.harvard.edu
O'Connor
Ryan P
RP
Wong
Sydney E
SE
D000818:Animals; D001921:Brain; D002404:Catheterization / Q000295:instrumentation / Q000379:methods*; D002491:Central Nervous System Agents / Q000008:administration & dosage*; D007260:Infusion Pumps; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D002491:Central Nervous System Agents