title: Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors.
abstract: An extremely sensitive procedure for the isolation and detection of DNA from bacterial cell cultures is described. Direct asymmetric PCR amplified products from E. coli cultures are specifically detected, at a concentration level as low as 0.01 cfu mL(-1) (cfu, colony forming unit), using disposable magnetic DNA hybridization amperometric sensors with no need for culture preconcentration steps.
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Madrid, E-28040, Spain.
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Loaiza
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.forename: María
authors.initials: M
authors.lastname: Pedrero
authors.forename: Pedro
authors.initials: P
authors.lastname: García
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D015374:Biosensing Techniques; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004926:Escherichia coli / Q000235:genetics*; D006046:Gold; D008280:Magnetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D006046:Gold