Characterization of different diamond-like carbon electrodes for biosensor design.
Diamond-like carbon (DLC) films are gaining big interest in electrochemistry research area. DLC electrodes made with different ratio of sp(3)/sp(2) carbon hybridization or doped with different percentages of nickel were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for the elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutaraldehyde. Measurements were made at a fixed potential+1.0V in 40mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose, 0.6875muA/mM, than SiCNi5%, 0.3654muA/mM. Detections limits were 20muM and 30muM, respectively. Apparent Michaelis-Menten constants were found around 3mM. Forty-eight percent and 79% of the original response for 0.5mM glucose remained after 10 days for both biosensors, respectively.
Maalouf
R
R
Center of Electrical Engineering of Lyon, CEGELY, UMR/CNRS 5005, Ecole Centrale Lyon, 69134 Ecully Cedex, France; Laboratory of Chemistry, Lebanese University, Fanar, Beirut, Lebanon; Laboratory of Analytical Electrochemistry, Claude Bernard University, Lyon I, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex, France.
Chebib
H
H
Saikali
Y
Y
Vittori
O
O
Sigaud
M
M
Garrelie
F
F
Donnet
C
C
Jaffrezic-Renault
N
N