title: Klugiella xanthotipulae gen. nov., sp. nov., a novel member of the family Microbacteriaceae.
abstract: An actinobacterium, designated strain 44C3(T), was isolated in Michigan, USA, from the hindgut of the larvae of Tipula abdominalis, an aquatic crane fly, and was subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain represented a separate clade within the family Microbacteriaceae. It showed highest 16S rRNA gene sequence similarity with Cryobacterium psychrotolerans 0549(T) (96.5 %). Strain 44C3(T) had a novel B-type peptidoglycan. The peptidoglycan contained the diamino acid lysine, the peptide Gly-d-Glu was detected in the partial hydrolysate and alanine was the N terminus of the interpeptide bridge. No other amino acids found in other B-type peptidoglycans (including diaminobutyric acid, ornithine, homoserine and hydroxyglutamic acid) could be detected. The major menaquinones were MK-12 and MK-11, the major fatty acids were ai-C(15 : 0), ai-C(17 : 0) and i-C(16 : 0) and the DNA G+C content was 60.9 mol%. Analysis of the chemotaxonomic and phylogenetic data suggested that strain 44C3(T) represented a novel species of a new genus within the family Microbacteriaceae, for which the name Klugiella xanthotipulae gen. nov., sp. nov. is proposed. The type strain of Klugiella xanthotipulae is 44C3(T) (=DSM 18031(T) =ATCC BAA-1524(T)).
authors.affiliation: Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Dana M
authors.initials: DM
authors.lastname: Cook
authors.forename: Emily DeCrescenzo
authors.initials: ED
authors.lastname: Henriksen
authors.forename: Theresa E
authors.initials: TE
authors.lastname: Rogers
authors.forename: Joy Doran
authors.initials: JD
authors.lastname: Peterson
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D000818:Animals; D004175:Diptera / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D012336:RNA, Ribosomal, 16S