BV2 enhanced the neurotrophic functions of mesenchymal stem cells after being stimulated with injured PC12.
OBJECTIVE
Immune-related mechanisms are involved in various neurological diseases and although mesenchymal stem cells (MSCs) have been shown to repair and replace injured neurons, it remains uncertain what the immune cells in the brain do during this process. Microglial cells are the representatives of immune cells in the brain. Microglial cells, injured neurons and stem cells often coexist in the same microenvironment. To investigate the effect of microglial cells on stem cells in such an environment, we tried to see how microglial cells affect the neurotrophicity of MSCs after being stimulated with injured neurons.


METHODS
Microglial cells (BV2) were stimulated by being cocultured with various sets of injured neurons (neuron cell pheochromocytoma, PC12), and we cocultured stimulated BV2 with MSCs whose supernatant was applied to injured PC12 to see if PC12 apoptosis was reduced. Neurotrophic factors in MSCs' supernatant, including basic fibroblast growth factor, brain-derived neurotrophic factors and nerve growth factors, were detected.


RESULTS
We found that after coculturing with injured PC12, BV2 were able to promote the neurotrophic functions of MSCs. The supernatant of MSCs cocultured with stimulated BV2 reduced PC12 apoptosis significantly compared with control, and had the highest basic fibroblast growth factor concentration, which was also significantly different from the control.


CONCLUSIONS
Our results showed that after being stimulated with injured neurons, microglial cells enhanced the neurotrophic functions of MSCs.
Luo
Xiaoguang
X
Department of Neurology, First Affiliated Hospital, China Medical University.
Ge
Chunlin
C
Ren
Yan
Y
Zhou
Jin
J
Li
Xiaolei
X
Yan
Rong
R
Zhang
Chaodong
C
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004660:Encephalitis / Q000276:immunology / Q000503:physiopathology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism*; D017628:Microglia / Q000276:immunology*; D020932:Nerve Growth Factor / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D009416:Nerve Regeneration / Q000187:drug effects / Q000276:immunology; D009474:Neurons / Q000187:drug effects / Q000276:immunology*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology
D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D016222:Fibroblast Growth Factor 2; D020932:Nerve Growth Factor