Maternal prenatal undernutrition alters the response of POMC neurons to energy status variation in adult male rat offspring.
Epidemiological studies suggest that maternal undernutrition predisposes the offspring to development of energy balance metabolic pathologies in adulthood. Using a model of a prenatal maternal 70% food-restricted diet (FR30) in rats, we evaluated peripheral parameters involved in nutritional regulation, as well as the hypothalamic appetite-regulatory system, in nonfasted and 48-h-fasted adult offspring. Despite comparable glycemia in both groups, mild glucose intolerance, with a defect in glucose-induced insulin secretion, was observed in FR30 animals. They also exhibited hyperleptinemia, despite similar visible fat deposits. Using semiquantitative RT-PCR, we observed no basal difference of hypothalamic proopiomelanocortin (POMC) and neuropeptide Y (NPY) gene expression, but a decrease of the OB-Rb and an increase of insulin receptor mRNA levels, in FR30 animals. These animals also exhibited basal hypercorticosteronemia and a blunted increase of corticosterone in fasted compared with control animals. After fasting, FR30 animals showed no marked reduction of POMC mRNA levels or intensity of beta-endorphin-immunoreactive fiber projections. By contrast, NPY gene expression and immunoreactive fiber intensity increased. FR30 rats also displayed subtle alterations of food intake: body weight-related food intake was higher and light-dark phase rhythm and refeeding time course were modified after fasting. At rest, in the morning, hyperinsulinemia and a striking increase in the number of c-Fos-containing cells in the arcuate nucleus were observed. About 30% of the c-Fos-expressing cells were POMC neurons. Our data suggest that maternal undernutrition differently programs the long-term appetite-regulatory system of offspring, especially the response of POMC neurons to energy status and food intake rhythm.
Breton
Christophe
C
Neurosciences et Physiologie Adaptatives, Université de Lille I, Villeneuve d'Ascq, France. christophe.breton@univ-lille1.fr
Lukaszewski
Marie-Amélie
MA
Risold
Pierre-Yves
PY
Enache
Mihaela
M
Guillemot
Johann
J
Rivière
Guillaume
G
Delahaye
Fabien
F
Lesage
Jean
J
Dutriez-Casteloot
Isabelle
I
Laborie
Christine
C
Vieau
Didier
D
D000818:Animals; D001066:Appetite / Q000502:physiology; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology / Q000502:physiology; D001823:Body Composition / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D004435:Eating / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D048070:Fetal Nutrition Disorders / Q000378:metabolism*; D016762:Genes, fos / Q000502:physiology; D005951:Glucose Tolerance Test; D006728:Hormones / Q000097:blood; D007328:Insulin / Q000097:blood; D008098:Litter Size; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000235:genetics; D054411:Receptors, Leptin / Q000235:genetics; D001615:beta-Endorphin / Q000378:metabolism
D006728:Hormones; D007328:Insulin; D009478:Neuropeptide Y; D054411:Receptors, Leptin; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; D011972:Receptor, Insulin