Assessment of myocardial blood perfusion improved by CD151 in a pig myocardial infarction model.
OBJECTIVE
To appraise the efficacy of CD151-induced myocardial therapeutic angiogenesis in a pig myocardial infarction model.


METHODS
CD151 and anti-CD151 were constructed into the recombinant adeno-associated virus (rAAV) vector. All 26 pigs were subjected to coronary artery ligation or no surgery. Eight weeks after coronary artery ligation, the expression of CD151 was measured by Western blot and immunostaining. Capillary density was evaluated using immunostaining for von Willebrand factor (vWF). 13N-labeled NH3 positron emission computed tomography ([13N]NH3PET) was measured to assess regional myocardial perfusion and the defect area.


RESULTS
CD151 gene delivery could increase the expression of CD151 at protein level. Over-expression of CD151 increased the density of total capillaries in the ischemic myocardium, significantly improved the blood perfusion and reduced the defect area percentage.


CONCLUSIONS
This study demonstrated that the rAAV-mediated CD151 gene delivery promoted efficient neovascularization and increased the blood perfusion after myocardial infarction in pigs.
Zuo
Hou-juan
HJ
Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Zheng-xiang
ZX
Liu
Xiao-chun
XC
Yang
Jun
J
Liu
Tao
T
Wen
Sha
S
Wang
Dao-wen
DW
Zhang
Xin
X
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use*; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D034421:Sus scrofa; D060185:Tetraspanin 24
D015703:Antigens, CD; C554715:CD151 protein, human; D060185:Tetraspanin 24