title: Expanding the scope of chemoselective peptide ligations in chemical biology.
abstract: Chemoselective ligation methods have increased the efficiency of bioconjugation, enabling complex macromolecules to be assembled. In particular, these methods have been utilized for the ligation and modification of peptides and proteins. The chemical synthesis of proteins from unprotected peptide fragments has enabled the introduction of unnatural amino acids, site-specific isotopic labeling, and the site-specific attachment of affinity tags or labels for imaging. A greater insight into current ligation methods has led to higher reaction rates, higher reaction yields, and greater biocompatibility, thereby increasing the impact of chemoselective ligation reactions in chemical biology.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Anouk
authors.initials: A
authors.lastname: Dirksen
authors.forename: Philip E
authors.initials: PE
authors.lastname: Dawson
mesh_terms: D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D013455:Sulfur / Q000737:chemistry
chemical_list: D009930:Organic Chemicals; D010446:Peptide Fragments; D010455:Peptides; D013455:Sulfur