[Effector function of CLA(+) T lymphocytes on autologous keratinocytes in psoriasis].
BACKGROUND
Cutaneous lymphocyte antigen (CLA) is expressed by a subgroup of memory T cells that exhibit skin homing and are implicated in cutaneous T-cell-mediated diseases.


METHODS
Expression of genes associated with psoriasis was analyzed in keratinocytes taken from patients and healthy individuals and cultured under different conditions, including activation using supernatants from CLA(+) T lymphocytes activated with anti-CD3 and anti-CD28 antibodies.


RESULTS
Keratinocytes from psoriasis patients activated by CLA(+)T lymphocytes expressed higher levels of interferon-inducible protein 10, HLA-DR, intercellular cell adhesion molecule 1, and inducible nitric oxide synthase.


CONCLUSIONS
Our results suggest that we have developed an in vitro model that will allow analysis of the effector role of CLA(+) T lymphocytes on keratinocytes in psoriasis. This model may allow the identification of genes involved in the pathology of psoriasis through induction by CLA(+) T lymphocytes.
Ferran
M
M
Servicio de Dermatología, Hospital del Mar-IMAS/IMIM, Barcelona, España. mferran@imas.imim.es
Giménez-Arnau
A M
AM
Bellosillo
B
B
Pujol
R M
RM
Santamaría-Babi
L F
LF
D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D006801:Humans; D015603:Keratinocytes / Q000276:immunology*; D008562:Membrane Glycoproteins; D011565:Psoriasis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; C063419:CTAGE1 protein, human; D008562:Membrane Glycoproteins