Phenotypic and genetic diversity of the traditional Lister smallpox vaccine.
As an initial step in the development of a second-generation smallpox vaccine derived from the Lister strain, to be prepared for a variola virus threat, diversity of the traditional vaccine was examined by characterizing a series of ten viral clones. In vitro and in vivo phenotypic studies showed that the biological behavior of the clones diverged from each other and in most cases diverged from the vaccinia virus (VACV) Lister parental population. Taken together, these results demonstrate the heterogeneity of the viral population within the smallpox vaccine and highlight the difficulty in choosing one clone which would meet the current requirements for a safe and effective vaccine candidate.
Garcel
Aude
A
Laboratoire de Virologie, Centre de Recherches du Service de Santé des Armées Emile Pardé, 24 avenue des Maquis du Grésivaudan, BP87, Grenoble 38702, France.
Perino
Julien
J
Crance
Jean-Marc
JM
Drillien
Robert
R
Garin
Daniel
D
Favier
Anne-Laure
AL
D000818:Animals; D001835:Body Weight; D001921:Brain / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D017385:Sequence Homology; D012899:Smallpox / Q000517:prevention & control; D012900:Smallpox Vaccine / Q000235:genetics* / Q000276:immunology*; D016019:Survival Analysis; D014616:Vaccinia virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010948:Viral Plaque Assay; D014779:Virus Replication
D012900:Smallpox Vaccine