title: From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel.
abstract: This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.
authors.affiliation: David Geffen School of Medicine, University of California, Los Angeles, USA.
authors.forename: Ivan
authors.initials: I
authors.lastname: Khvorostov
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Michael
authors.initials: M
authors.lastname: Teitell
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D004338:Drug Combinations; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007797:Laminin; D051379:Mice; D011509:Proteoglycans
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen