title: Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil.
abstract: The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic B. fragilis (NTBF) among 109 strains isolated from 1980-2008 in Brazil were investigated by PCR. One strain, representing 0.9% of the total analyzed strains, harbored the bft gene which was identified as bft-1 isoform based on PCR-RFLP and sequencing. Forty-nine strains (44.9%) exhibited the NTBF pattern III which possesses the flanking region required for pathogenicity island acquisition in which the bft gene is codified. These data reinforce the potential of B. fragilis as an emerging enteropathogen in our country.
authors.affiliation: Laboratório de Biologia de Anaeróbios, Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil. karlarodr@yahoo.com.br
authors.forename: Karla R
authors.initials: KR
authors.lastname: Miranda
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Dias
authors.forename: Priscilla L S
authors.initials: PL
authors.lastname: Guimarães
authors.forename: Renata F
authors.initials: RF
authors.lastname: Boente
authors.forename: Heidi
authors.initials: H
authors.lastname: Pauer
authors.forename: Priscila Z
authors.initials: PZ
authors.lastname: Ramos
authors.forename: Laís S
authors.initials: LS
authors.lastname: Falcão
authors.forename: Eliane de O
authors.initials: Ede O
authors.lastname: Ferreira
authors.forename: Ilana T
authors.initials: IT
authors.lastname: Balassiano
authors.forename: Livia Q
authors.initials: LQ
authors.lastname: Ferreira
authors.forename: Joaquim dos
authors.initials: Jd
authors.lastname: Santos-Filho
authors.forename: Geraldo R de
authors.initials: GR
authors.lastname: Paula
authors.forename: Eduardo N F
authors.initials: EN
authors.lastname: Antunes
authors.forename: Katia E S
authors.initials: KE
authors.lastname: Avelar
authors.forename: Regina M C P
authors.initials: RM
authors.lastname: Domingues
mesh_terms: D001441:Bacteroides fragilis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D001938:Brazil; D004768:Enterotoxins / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004768:Enterotoxins