Characterization of two isoforms of Japanese spiny lobster Panulirus japonicus defensin cDNA.
Antimicrobial peptides (AMPs) are components of the innate immune responses that form the first line of host defense against pathogens. In this study, cDNAs of two new isoforms of defensin (designated PJD1 and PJD2) from a Japanese spiny lobster Panulirus japonicus haemocytes cDNA library were cloned and sequenced. PJD1 and PJD2 consist of 656 and 673 nucleotides encoding putative proteins of 66 and 64 amino acids, respectively. The isoforms share 74.2% amino acid identity. In a phylogenetic analysis, the peptides clustered with vertebrate defensins and were closely mostly related to chicken beta-defensin. PJD1 and PJD2 were detected in all tissues examined including heart, nerves, intestine, haemocytes, gills and hepatopancreas.
Pisuttharachai
Duangjai
D
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Yasuike
Motoshige
M
Aono
Hideaki
H
Yano
Yutaka
Y
Murakami
Keisuke
K
Kondo
Hidehiro
H
Aoki
Takashi
T
Hirono
Ikuo
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D023082:Defensins / Q000145:classification / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D033521:Palinuridae / Q000276:immunology*; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment
D018076:DNA, Complementary; D023082:Defensins; D020033:Protein Isoforms