title: Mathematical model of the Lux luminescence system in the terrestrial bacterium Photorhabdus luminescens.
abstract: A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations. This model will have value in the interpretation of Lux data when used as a reporter in time-course gene expression experiments. The system was tested on time series and stationary data from published papers and the model is in good agreement with the published data. Metabolic control analysis demonstrates that control of the system lies mainly with the aldehyde recycling pathway (LuxE and LuxC). The rate at which light is produced in the steady state model shows a low sensitivity to changes in kinetic parameter values to those measured in other species of luminescent bacteria, demonstrating the robustness of the Lux system.
authors.affiliation: University of Birmingham, Birmingham, UKB15 2TT. PAW631@bham.ac.uk
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Welham
authors.forename: Dov J
authors.initials: DJ
authors.lastname: Stekel
mesh_terms: D055162:Biocatalysis; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D020637:Photorhabdus / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D008164:Luminescent Proteins