[Study on the validation of application model on embryonic stem test].
OBJECTIVE
According trend of differentiation cardiomyocyte of embryonic stem cell,the model of EST has been built and the validation of the model is needed to be tested. It is profit to improve the method of safety evaluation.


METHODS
The embryonic stem cell differentiates into cardiomyocyte in different concentrations of Penicillin G,DPH and 5-FU with hanging and suspending culture conditions. With the results of cytotoxicity, the different embryonic toxicity characteristics of different substants may be detected clearly.


RESULTS
The ID50 (D3) concentration of three embryo toxicants were 1099, 47.4 and 0.023 microg/ml. Penicillin G, DPH and 5-FU were discriminated as none-embryotoxicity, weak-embryotoxicity and strong-embryotoxicity.


CONCLUSIONS
Test compounds between three classes were discriminated correctly .The validation of EST model that established by us is high.
Yu
Zhou
Z
Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China.
Yan
Weixing
W
Zhang
Lianfeng
L
Xu
Haibin
H
D000826:Animal Testing Alternatives / Q000379:methods / Q000592:standards*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D005472:Fluorouracil / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D010400:Penicillin G / Q000633:toxicity; D015203:Reproducibility of Results; D018675:Toxicity Tests / Q000379:methods*
D010400:Penicillin G; D005472:Fluorouracil