title: Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil.
abstract: The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.
authors.affiliation: Centro de Pesquisa em Medicina Tropical, Porto Velho, RO, Brasil.
authors.forename: Tatiane
authors.initials: T
authors.lastname: Silva
authors.forename: Paulo Afonso
authors.initials: PA
authors.lastname: Nogueira
authors.forename: Gleiciene Félix
authors.initials: GF
authors.lastname: Magalhães
authors.forename: Andréa Fagundes
authors.initials: AF
authors.lastname: Grava
authors.forename: Luiz Hildebrando Pereira da
authors.initials: LH
authors.lastname: Silva
authors.forename: Patrícia Puccinelli
authors.initials: PP
authors.lastname: Orlandi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D003092:Colitis / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D015994:Incidence; D007223:Infant; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012760:Shigella / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial