[Extraction of triterpenoids from fruiting [corrected] bodies of Ganoderma lucidum by supercritical fluid extraction].
OBJECTIVE
To optimize the extracting condition for triterpenoids from the fruits of Ganoderma lucidum by supercritical fluid extraction (SFE).


METHODS
Optimum extraction conditions were studied by orthogonal tests. The total triterpenoids were determined by ultraviolet spectrophotometry and ganoderic acid B was determined by RP-HPLC.


RESULTS
The optimal extraction conditions were that the pressure 25 MPa, the temperature was 45 degrees C, the extraction time was 1.5 hour, and the ethanol was employed as modifier carrier at the volume of 3 mL x g(-1).


CONCLUSIONS
SFE is superior and it is feasible to extract triterpenoids from fruits of Ganoderma lucidum.
Song
Shi-hua
SH
Jiangsu Provincial Academy of Chinese Medicine, Jiangsu Engineering Research Center for Pharmaceutical Preparation, Nanjing 210028, China.
Jia
Xiao-bin
XB
Chen
Yan
Y
Chen
Bin
B
Wang
Li-jing
LJ
Zhao
Cheng-lei
CL
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D020940:Reishi / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000737:chemistry*
D014315:Triterpenes