Ion mobility-mass spectrometer interface for collisional activation of mobility separated ions.
An ion mobility-mass spectrometer (IM-MS) interface is described that can be employed to perform collisional activation and/or collision-induced dissociation (CID) with good transmission of mobility separated ions to the MS analyzer. The IM-MS interface consists of a stacked-ring ion guide design, where the field strength and pressure ratio can be operated such that structural rearrangement reactions and/or CID are achieved as a function of the effective ion temperature. The ion dynamics and collisional activation processes in the IM-MS interface are described as a function of the ion-neutral collisions, ion kinetic energies, and effective ion temperature. The applicability of the IM-CID-MS methodology to studies of peptide ion fragmentation is illustrated using a series of model peptides.
Fernandez-Lima
Francisco A
FA
Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
Becker
Christopher
C
Gillig
Kent J
KJ
Russell
William K
WK
Tichy
Shane E
SE
Russell
David H
DH
D000595:Amino Acid Sequence; D007477:Ions / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D007477:Ions; D010455:Peptides