Application of centrifugal partition chromatography coupled with evaporative light scattering detection for the isolation of saikosaponins-a and -c from Bupleurum falcatum roots.
Centrifugal partition chromatography (CPC) coupled with evaporative light scattering detection (ELSD) was applied to separate saikosaponins-a and -c preparatively from Bupleurum falcatum roots. The two-phase solvent system composed of ethyl acetate/n-butanol/methanol/water (15:1:3:15 by volume) was used to yield saikosaponins-a (36.1 mg) and -c (28.7 mg) from 370 mg of saponin-rich extract. The purities of isolated compounds were 96.6 and 97.3% for saikosaponins-a and -c, respectively. Structure identification of these compounds was accomplished by comparison of spectroscopic data of ESI-MS, 1H NMR, and 13C NMR with those of previously reported values.
Yoon
Kee Dong
KD
College of Pharmacy, and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Kim
Jinwoong
J
D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000302:isolation & purification*; D012542:Scattering, Radiation; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D