title: A splice variant of the Neurospora crassa hex-1 transcript, which encodes the major protein of the Woronin body, is modulated by extracellular phosphate and pH changes.
abstract: The Woronin body, a septal pore-associated organelle specific to filamentous ascomycetes, is crucial for preventing cytoplasmic bleeding after hyphal injury. In this study, we show that T1hex-1 transcript and a variant splicing T2hex-1 transcript are up-regulated at alkaline pH. We also show that both hex-1 transcripts are overexpressed in the preg(c), nuc-1(RIP), and pacC(ko) mutant strains of Neurospora crassa grown under conditions of phosphate shortage at alkaline pH, suggesting that hex-1 transcription may be coregulated by these genes. In addition, we present evidence that N. crassa PacC also has metabolic functions at acidic pH.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Leal
authors.forename: Fábio M
authors.initials: FM
authors.lastname: Squina
authors.forename: Janaína S
authors.initials: JS
authors.lastname: Freitas
authors.forename: Emiliana M
authors.initials: EM
authors.lastname: Silva
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Ono
authors.forename: Nilce M
authors.initials: NM
authors.lastname: Martinez-Rossi
authors.forename: Antonio
authors.initials: A
authors.lastname: Rossi
mesh_terms: D017398:Alternative Splicing; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D009492:Neurospora crassa / Q000235:genetics* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D005656:Fungal Proteins; D010710:Phosphates; C426803:Woronin body major protein, Neurospora crassa