title: Purification of astaxanthin from mutant of Phaffia rhodozyma JH-82 which isolated from forests trees of Iran.
abstract: Astaxanthin have been extracted and purified from mutant isolate of Phaffia rhodozyma JH-82. Purified astaxanthin was identified by spectrophotometric, TLC and HPLC analysis and were compared with synthetic astaxanthin. Results of TLC analysis indicated that isolate of P. rhodozyma JH-82 were able to produce nine different carotenoids and high level of carotenoids was belong to astaxanthin. Results of this study for pure astaxanthin production indicated that mutant of JH-82 of P. rhodozyma (230 microg g(-1)(dried yeast)) produced more astaxanthin than natural isolate JH-80 (140 microg g(-1)(dried yeast)). The HPLC spectrum showed retention time 11 min for both purified and synthetic astaxanthin and solvent was CDCl3.
authors.affiliation: Department of Biomedical Sciences, Woman Research Center, Alzahra University, Tehran, Iran.
authors.forename: Shokufeh
authors.initials: S
authors.lastname: Golkhoo
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Barantalab
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Ahmadi
authors.forename: Zuhair Muhammad
authors.initials: ZM
authors.lastname: Hassan
mesh_terms: D001487:Basidiomycota / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007492:Iran; D009154:Mutation; D012015:Reference Standards; D013053:Spectrophotometry / Q000379:methods; D014197:Trees / Q000382:microbiology*; D024341:Xanthophylls / Q000302:isolation & purification
chemical_list: D024341:Xanthophylls; C005948:astaxanthine