Identification and characterization of Class 1 integron resistance gene cassettes among Salmonella strains isolated from imported seafood.
A total of 210 Salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. Class 1 integrons from three multidrug-resistant Salmonella enterica strains (Salmonella enterica serovars Newport [strain 62], Typhimurium var. Copenhagen [strain 629], and Lansing [strain 803], originating from Hong Kong, the Philippines, and Taiwan, respectively) were characterized. Southern hybridization of plasmids isolated from these strains, using a class 1 integron probe, showed that trimethoprim-sulfamethoxazole and streptomycin resistance genes were located on a megaplasmid in strain 629. Our study indicates that imported seafood could be a reservoir for Salmonella isolates resistant to multiple antibiotics.
Khan
Ashraf A
AA
Division of Microbiology, U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079, USA. Ashraf.khan@fda.hhs.gov
Ponce
Elizabeth
E
Nawaz
M S
MS
Cheng
Chorng-Ming
CM
Khan
Junaid A
JA
West
Christine S
CS
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006723:Hong Kong; D032023:Integrons; D008969:Molecular Sequence Data; D010679:Philippines; D010957:Plasmids; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D017747:Seafood / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013624:Taiwan
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial