Ovine colostrum nanopeptide affects amyloid beta aggregation.
A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the PRP complex components can affect aggregation of amyloid beta (Abeta1-42). The effect of NP on Abeta aggregation was studied using Thioflavin T (ThT) binding, atomic force microscopy, and analyzing circular dichroism spectra. Results presented suggest that NP can directly interact with amyloid beta, inhibit its aggregation and disrupt existing aggregates acting as a beta sheet breaker and reduce toxicity induced by aggregated forms of Abeta.
Janusz
Maria
M
Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. janusz@immuno.iitd.pan.wroc.pl
Woszczyna
Mirosław
M
Lisowski
Marek
M
Kubis
Adriana
A
Macała
Józefa
J
Gotszalk
Teodor
T
Lisowski
Józef
J
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D052160:Benzothiazoles; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D005456:Fluorescent Dyes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D017433:Protein Structure, Secondary / Q000187:drug effects; D051381:Rats; D034561:Sheep, Domestic / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry
D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D009842:Oligopeptides; D010446:Peptide Fragments; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C425383:valyl-glutamyl-seryl-tyrosyl-valyl-prolyl-leucyl-phenylalanylproline; C009462:thioflavin T