In-vitro gastric cancer prevention by a polyphenol-rich extract from olives through induction of apoptosis.
In recent years, natural dietary agents have drawn a great deal of attention owing to their demonstrated ability to suppress cancer. We aimed to investigate the in-vitro gastric cancer preventive activity of a methanol extract obtained from table olives of Greek origin. Tested were AGS cell proliferation, induction of apoptosis and inhibition of inflammation. AGS stomach cancer cells were cultured at a density of 10 cells/ml. Methanol extract of olive was added to cultures at concentrations of 2.0, 1.6, 1.0, and 0.4 microg phenols/ml. Effect on cellular viability was evaluated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and percentages of early and late apoptotic cells were assayed by annexin V-FITC staining on a FACS scan. Interleukin-8 (IL-8) and intercellular adhesion molecule (ICAM)-1 mRNA and protein production were measured by applying reverse transcriptase-PCR and enzyme-linked immunosorbent assay. Olive extract significantly suppressed cell proliferation at 2.0, 1.6, and 1.0 microg phenols/ml. Flow cytometric analysis of Annexin-V labeled cells indicated that 2.0 microg phenols/ml significantly induced apoptosis. Similarly, at 2.0, 1.6, and 1.0 microg phenols/ml a significant decrease of ICAM-1 and IL-8 protein levels was observed. ICAM-1, as well as IL-8, mRNA expression were decreased in the presence of 2.0 microg phenols/ml. Results indicate that the methanol extract from olives, rich in phenolic compounds, exhibits gastric cancer preventive efficacy by limiting cell proliferation, inducing cell death and suppressing inflammation in AGS cells.
Kountouri
Aggeliki M
AM
Department of Science of Dietetics-Nutrition, Harokopio University of Athens, Athens, Greece.
Kaliora
Andriana C
AC
Koumbi
Lemonica
L
Andrikopoulos
Nikolaos K
NK
D000230:Adenocarcinoma / Q000517:prevention & control*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000302:isolation & purification / Q000627:therapeutic use*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification / Q000627:therapeutic use*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D059808:Polyphenols; D013274:Stomach Neoplasms / Q000517:prevention & control*; D015854:Up-Regulation / Q000187:drug effects
D005419:Flavonoids; D016209:Interleukin-8; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D018799:Intercellular Adhesion Molecule-1