The rpoB gene as a tool for clinical microbiologists.
The rpoB gene, encoding the beta-subunit of RNA polymerase, has emerged as a core gene candidate for phylogenetic analyses and identification of bacteria, especially when studying closely related isolates. Together with the 16S rRNA gene, rpoB has helped to delineate new bacterial species and refine bacterial community analysis, as well as enabling the monitoring of rifampicin resistance-conferring mutations. Sequencing of rpoB enables efficient estimation of bacterial G+C% content, DNA-DNA hybridization value and average nucleotide identity (percentage of the total genomic sequence shared between two strains) when taxonomic relationships have been firmly established. New identification tools targeting a rpoB gene fragment located between positions 2300 and 3300 have been developed recently. Therefore, inclusion of the rpoB gene sequence would be useful when describing new bacterial species.
Adékambi
Toïdi
T
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS IRD UMR 6236 IFR 48, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
Drancourt
Michel
M
Raoult
Didier
D
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit