title: Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control.
abstract: The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.
authors.affiliation: Department of Food, Institution of Food safety, College of Light Industry and Food, South China University of Technology, Guangzhou, China.
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Xiao
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: He
authors.forename: Yi-Gang
authors.initials: YG
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Gu
authors.initials: G
authors.lastname: Chen
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Li
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Zhang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Liu
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Xiao-Quan
authors.initials: XQ
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000821:virology; D004770:Enterovirus / Q000302:isolation & purification / Q000502:physiology*; D029821:Enterovirus A, Human / Q000302:isolation & purification / Q000502:physiology*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral