title: Determination of glyphosate using off-line ion exchange preconcentration and capillary electrophoresis-laser induced fluorescence detection.
abstract: An enrichment method for the herbicide glyphosate is presented based on ion exchange solid phase extraction (SPE) technique. A 200-mul micro-pipette tip packed with 50mg of Bio-Rad AG1-X8 anion exchanger beads was used for offline extraction of glyphosate from 50ml of spiked river water sample. The retained glyphosate was eluted with 10mM HCl and then converted quantitatively to the corresponding amine (glycine) using hypochlorite. Subsequent fluorescent labeling using naphthalene-2,3-dicarboxaldehyde (NDA)-cyanide allowed micellar electrokinetic chromatography (MEKC) separation and laser-induced fluorescence detection (LIF) with a violet diode laser. Optimization of the sample clean-up, extraction, elution, conversion and labeling steps enabled analysis of glyphosate in river water in the nanomolar range. Detection limits were 0.04nM glyphosate in standards and 1.6nM in spiked river.
authors.affiliation: Department of Chemistry, Gunning/Lemieux Chemistry Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
authors.forename: Jiang
authors.initials: J
authors.lastname: Jiang
authors.forename: Charles A
authors.initials: CA
authors.lastname: Lucy