title: Sweet potato beta-carotene bioefficacy is enhanced by dietary fat and not reduced by soluble fiber intake in Mongolian gerbils.
abstract: Orange-fleshed sweet potato (OFSP) is an important source of beta-carotene (betaC). Provitamin A bioefficacy from plant foods is influenced by dietary fat and fiber. We fed 3% OFSP powder diets with varying amounts of fat and soluble fiber to vitamin A (VA)-depleted Mongolian gerbils (n = 85) for 3 wk (8 groups, n = 10/group; control, n = 9) following a baseline kill (n = 6). OFSP diets differing in fat (3, 6, and 12%) contained 0.24% soluble fiber. Two additional 3% OFSP diets contained 6% fat and 3 or 9% white-fleshed sweet potato (WFSP) powder with soluble fiber contents of 0.42 and 0.80%, respectively. Control, VA-, and betaC-supplemented groups were included. Simulated digestion experiments compared the bioaccessibility of betaC from boiled vs. oil stir-fried OFSP. All OFSP diets maintained VA status and 12% fat and WFSP-added diets improved VA status above baseline (P < 0.05). Bioefficacy, as bioconversion factors, in gerbils fed 12% fat (3.5 +/- 1.4 microg betaC:1 microg VA) was improved over the 3% fat and betaC groups (6.5 +/- 3.7 and 6.7 +/- 3.7 microg betaC:1 microg VA, respectively) (P < 0.05) but did not differ from WFSP-added groups or the 6% fat group with no WFSP. Stir-frying doubled the efficiency of betaC incorporation into micelles during small intestinal digestion in support of the stimulatory effect of dietary fat on bioefficacy in vivo. Soluble fiber intake derived from WFSP did not influence bioefficacy. Replacing WFSP with OFSP will affect VA status if adopted by target groups.
authors.affiliation: Interdepartmental Graduate Program in Nutritional Sciences, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Jordan P
authors.initials: JP
authors.lastname: Mills
authors.forename: Gaston A
authors.initials: GA
authors.lastname: Tumuhimbise
authors.forename: Kazi M
authors.initials: KM
authors.lastname: Jamil
authors.forename: Sagar K
authors.initials: SK
authors.lastname: Thakkar
authors.forename: Mark L
authors.initials: ML
authors.lastname: Failla
authors.forename: Sherry A
authors.initials: SA
authors.lastname: Tanumihardjo
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000032:analysis / Q000494:pharmacology*; D004043:Dietary Fiber / Q000032:analysis / Q000494:pharmacology*; D005849:Gerbillinae; D027723:Ipomoea batatas / Q000378:metabolism*; D012995:Solubility; D014801:Vitamin A / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000188:drug therapy*; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D004041:Dietary Fats; D004043:Dietary Fiber; D019207:beta Carotene; D014801:Vitamin A