Metabonomics and population studies: age-related amino acids excretion and inferring networks through the study of urine samples in two Italian isolated populations.
The study of two different Italian isolated populations was combined with a metabonomic approach to better understand tubular handling of amino acids. Levels of amino acids and metabolites have been analyzed by Nucleic Magnetic Resonance and expressed as ratio vs urinary creatinine concentration (mmol/mol). For most of the amino acids there is an age-related U shape pattern of excretion, with the peaks during childhood and old age, and a significant reduction in the adult age. Hierarchical cluster analysis has clearly identified three groups clustering the same amino acids: His, Thr and Ala (group one); Gly and Phe (group two) and a third larger one. Results have been further confirmed by factor and regression analysis, and used to confirm and, in some cases, infer new amino acids networks. As a matter of facts, the identification of strong evidences for clustering of urine excretion of several neutral amino acids suggests the predominant impact of relevant and common transporters.
D'Adamo
Pio
P
Medical Genetics, Department of Laboratory Medicine, Institute for Maternal and Child Health IRCCS-Burlo Garofolo, Trieste, Italy.
Ulivi
Sheila
S
Beneduci
Amerigo
A
Pontoni
Gabriele
G
Capasso
Giovambattista
G
Lanzara
Carmela
C
Andrighetto
Gilberto
G
Hladnik
Uros
U
Nunes
Virginia
V
Palacin
Manuel
M
Gasparini
Paolo
P
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000596:Amino Acids / Q000737:chemistry / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007558:Italy; D008297:Male; D055432:Metabolomics; D008875:Middle Aged; D044382:Population Groups; D012424:Rural Population; D055815:Young Adult
D000596:Amino Acids