Sensitive liquid chromatography-electrospray mass spectrometry allows for the analysis of the O-glycosylation of immunoprecipitated proteins from cells or tissues: application to MUC1 glycosylation in cancer.
We have analyzed the structures of the glycans on immunoprecipitated proteins from small amounts of cell or tissue lysates, by liquid-chromatography electrospray mass spectrometry (LC-ESI-MS) and MS/MS. The sensitive and specific method was applied to the analysis of the O-glycosylation of MUC1 in breast, prostate and gastric cancer, including analysis of a patient tumor specimen. The method will be applicable for the glycosylation analysis of individual proteins.
Bäckström
Malin
M
Institute of Biomedicine, Department of Medical Biochemistry, University of Gothenburg, Sweden. malin.backstrom@medkem.gu.se
Thomsson
Kristina A
KA
Karlsson
Hasse
H
Hansson
Gunnar C
GC
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D002853:Chromatography, Liquid / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry / Q000378:metabolism; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000378:metabolism; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
C060192:MUC1 protein, human; D018396:Mucin-1