title: Aryl sulfamates are broad spectrum inactivators of sulfatases: effects on sulfatases from various sources.
abstract: Aryl sulfamates were originally developed as inhibitors of steroid sulfatase, and have recently been shown to be powerful inactivators of a bacterial sulfatase, PaAtsA from Pseudomonas aeruginosa. We demonstrate that a simple aryl sulfamate, 3-nitrophenyl sulfamate, can inactivate sulfatases from various sources including snail, limpet and abalone. In each case inactivation was time-dependent and active-site directed, as demonstrated by protection against inactivation by substrate. These results suggest that such easily acquired aryl sulfamates can be used as reliable biochemical reagents for the study of sulfatases from a diverse array of sources.
authors.affiliation: School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Rd, 3010 Parkville, Vic., Australia.
authors.forename: Pavla
authors.initials: P
authors.lastname: Bojarová
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Williams
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D013045:Species Specificity; D013429:Sulfatases / Q000037:antagonists & inhibitors*; D013451:Sulfonic Acids / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D013451:Sulfonic Acids; C005741:sulfamic acid; D013429:Sulfatases