title: The effect of TiO2 nanotubes on endothelial function and smooth muscle proliferation.
abstract: In this study we investigate the effects of nanotubular titanium oxide (TiO(2)) surfaces on vascular cells. EC and VSMC response to nanotubes was investigated through immunofluorescence staining, scanning electron microscopy, 5-ethynyl-2'-deoxyuridine proliferation assays, and prostaglandin I(2) (PGI(2)) enzyme immunoassays. We found that the nanotubular surface significantly enhances EC proliferation and secretion of PGI(2). The surface also results in a decrease in VSMC proliferation and increased expression of smooth muscle alpha-actin. These data suggest that engineered nanotopographical cues may influence both EC and VSMC behavior in a manner that may be useful for stent or other vascular applications.
authors.affiliation: Department of Bioengineering and Therapeutic Sciences, University of California, Box 2520, Byers Hall Rm 203C, San Francisco, CA 94158-2330, USA.
authors.forename: Lily
authors.initials: L
authors.lastname: Peng
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Eltgroth
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: LaTempa
authors.forename: Craig A
authors.initials: CA
authors.lastname: Grimes
authors.forename: Tejal A
authors.initials: TA
authors.lastname: Desai
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000033:anatomy & histology / Q000502:physiology; D008422:Materials Testing; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology*; D043942:Nanotubes / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000378:metabolism
chemical_list: D000199:Actins; D001672:Biocompatible Materials; C009495:titanium dioxide; D014025:Titanium