Inflammatory mediators of cerebrospinal fluid from patients with spinal cord injury.
BACKGROUND
The guidelines of MP treatment for acute SCI are still under debate. We examined the inflammatory mediators of CSF in patients with SCI and assessed the effect of MP treatment.


METHODS
We studied 7 patients with acute SCI at the cervical level and examined the mediators of CSF in patients by cytokine antibody array, ELISA and gelatin zymography.


RESULTS
We found that levels of IL-6, IL-8, monocyte chemoattractant protein-1, neutrophil-activating peptide 2, intracellular adhesion molecule-1, soluble Fas, tissue inhibitors of metalloproteinase 1, and matrix metalloproteinases-2 and -9 were upregulated in patients with complete SCI without MP treatment as compared to patients with MP treatments, incomplete SCI, or controls. Nerve growth factor was upregulated in patients with MP treatment.


CONCLUSIONS
We suggest that a neuroinflammatory CSF profile after complete SCI could be suppressed with MP treatment via downregulating the expression of various cytokines.
Tsai
Ming-Cheng
MC
Department of Neurosurgery, Shin Kong Wu Ho-Su Memorial Hospital, and Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei 111, Taiwan.
Wei
Chih-Peng
CP
Lee
Dan-Yang
DY
Tseng
Yi-Tzu
YT
Tsai
Ming-Dar
MD
Shih
Yung-Luen
YL
Lee
Yi-Hsuan
YH
Chang
Shwu-Fen
SF
Leu
Sy-Jye
SJ
D000328:Adult; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D016207:Cytokines / Q000134:cerebrospinal fluid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000134:cerebrospinal fluid*; D008297:Male; D020782:Matrix Metalloproteinases / Q000134:cerebrospinal fluid; D008775:Methylprednisolone / Q000627:therapeutic use; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics; D019635:Neurosurgical Procedures; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000601:surgery; D055815:Young Adult
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D009414:Nerve Growth Factors; D020782:Matrix Metalloproteinases; D008775:Methylprednisolone