Correlative transmission microscopy: cytochemical localization and immunocytochemical localization in studies of oxidative and nitrosative stress.
Microscopy studies of oxidative stress have often consisted of only immunocytochemical localization or only cytochemical localization studies. However, correlative studies on the same sections provide more useful data in interpreting oxidative and nitrosative stress. Cellular sites of superoxide and hydrogen peroxide production can be identified using cerium-based cytochemical localization of NADH oxidase enzymatic activity, while areas of nitrosative stress can be identified by immunocytochemical localization of nitrotyrosine in the same sections of tissue.
Ellis
E Ann
EA
Microscopy and Imaging Center, Texas A&M University, College Station, Texas.
D000818:Animals; D006861:Hydrogen Peroxide / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D016707:Tissue Fixation; D009927:Tissue and Organ Procurement; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D009097:Multienzyme Complexes; D026361:Reactive Nitrogen Species; D013481:Superoxides; C002744:3-nitrotyrosine; D014443:Tyrosine; D006861:Hydrogen Peroxide; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases