title: NP7 protects from cell death induced by oxidative stress in neuronal and glial midbrain cultures from parkin null mice.
abstract: Parkin mutations produce Parkinson's disease (PD) in humans and nigrostriatal dopamine lesions related to increased free radicals in mice. We examined the effects of NP7, a synthetic, marine derived, free radical scavenger which enters the brain, on H(2)O(2) toxicity in cultured neurons and glia from wild-type (WT) and parkin null mice (PK-KO). NP7, 5-10 microM, prevented the H(2)O(2) induced apoptosis and necrosis of midbrain neuronal and glial cultures from WT and PK-KO mice. NP7 suppressed microglial activation and the H(2)O(2) induced drop-out of dopamine neurons(.) Furthermore, NP7 prevented the increased phosphorylation of ERK and AKT induced by H(2)O(2). NP7 may be a promising neuroprotector against oxidative stress in PD.
authors.affiliation: Departmento de Neurobiología-Investigación, CIBERned, Hospital Ramón y Cajal, Madrid, Spain. maria.a.mena@hrc.es
authors.forename: M A
authors.initials: MA
authors.lastname: Mena
authors.forename: M J
authors.initials: MJ
authors.lastname: Casarejos
authors.forename: R
authors.initials: R
authors.lastname: Solano
authors.forename: J A
authors.initials: JA
authors.lastname: Rodríguez-Navarro
authors.forename: A
authors.initials: A
authors.lastname: Gómez
authors.forename: I
authors.initials: I
authors.lastname: Rodal
authors.forename: M
authors.initials: M
authors.lastname: Medina
authors.forename: J G
authors.initials: JG
authors.lastname: de Yebenes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D000978:Antiparkinson Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019610:Cytoprotection; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D018345:Mice, Knockout; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics
chemical_list: D000975:Antioxidants; D000978:Antiparkinson Agents; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D006861:Hydrogen Peroxide; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein