One-port video assisted laparoscopic kidney biopsy in standing steers.
The purpose of this study was to simplify the two-port laparoscopic renal biopsy technique used in support of pharmacokinetic studies through the application of a one-port system. Twelve Holstein steers were fasted for 24 h and sedated with acepromazine and xylaxine in preparation for laparoscopic surgery in standing stocks. Lidocaine 2% was injected to provide local anesthesia for introduction of the trocar-cannula assembly. The operating endoscope was inserted and the abdomen was insufflated with CO(2). A biopsy forceps was introduced into the channel of the operating endoscope to obtain a 100mg kidney cortex sample. Eighteen laparoscopic procedures provided 18 kidney samples suitable for pharmacokinetic studies. No complications were encountered. The one-port laparoscopic kidney biopsy is feasible and safe, and advanced skill required for triangulation is not necessary for its performance.
Chiesa
Oscar Alberto
OA
Center for Veterinary Medicine, Office of Research, Division of Residue Chemistry, Food and Drug Administration, 8401 Muirkirk Road, Laurel, MD 20708, USA.
von Bredow
Jurgen
J
Smith
Michelle
M
Thomas
Michael
M
D000818:Animals; D001706:Biopsy / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D007668:Kidney / Q000601:surgery*; D020706:Laparoscopes / Q000662:veterinary*; D010535:Laparoscopy / Q000379:methods / Q000662:veterinary*; D008297:Male; D014743:Videotape Recording