[Anti-estrogenic effect of dioxin on rat skeleton development].
OBJECTIVE
To investigate the teratogenic and anti-estrogenic effect of environment teratogen on rat skeleton development.


METHODS
The fetal rat models with congenital skeleton malformation were constructed with 2, 3, 7,8-tetrachlorodibenzo-p-dioxin (TCDD) on pregnant day 10. The transparent skeleton of fetal rat was made with alizarin bordeaux. The cell pathology of chondrocyte in fetal rat center of ossification was observed by light microscope. The estradiol in rat serum was measured by radioimmunity method.


RESULTS
Treated with 5-15 microg/kg TCDD, the single or multiple rat fetal skeletal development malformation had been induced. The teratogenic effect of TCDD on the osteogenesis was in dose-dependent manner. There were decreased center of ossification and cell degeneration of chondrocyte in fetal extremity limbs. The estradiol increased significantly in TCDD-treated rat, compared with normal rat (P < 0.05).


CONCLUSIONS
TCDD can induce congenital fetal skeletal malformation under interfering the biological activity of estrogen and the function of chondrocyte and the primary center of ossification.
Guo
Lei
L
Department of Orthopedic Surgery, Department of endocrinology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhao
Yuyan
Y
Zhang
Shiliang
S
Liu
Kui
K
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D001842:Bone and Bones / Q000002:abnormalities* / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D005260:Female; D005315:Fetal Diseases / Q000139:chemically induced; D005333:Fetus; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity
D052244:Endocrine Disruptors; D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; D004958:Estradiol