Assessment of a dynamic hollow-fibre liquid phase microextraction system for human blood plasma samples.
A dynamic liquid phase microextraction (LPME) system, based on hollow-fibre supported liquid membrane (SLM) extraction, was developed for extracting ionisable xenobiotics from human plasma, and its performance was evaluated (in terms of extraction efficiency, reproducibility, durability and carry-over) using nitrophenolic compounds as model analytes at concentrations of 0.1-0.5 microg mL(-1) in aqueous standards. The efficiency and repeatability were tested also on spiked human plasma. The system is non-expensive, convenient, requires minimal manual handling and enables samples with volumes as small as 0.2 mL to be extracted. For plasma samples extraction efficiencies of between 30 and 58% were achieved within 20 min, including washing steps. The limit of detection (LOD) values were in the range 0.02-0.03 microg mL(-1). The developed system can provide enrichment factors up to eight, based on the injection-to-acceptor volume ratio (in this case 0.2-0.025 mL). The same hollow-fibre membrane was used up to 8 days with no loss of efficiency. Carry-over was lower than detection limit.
Hansson
Helena
H
Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden.
Nilsson
Ulrika
U
D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D010636:Phenols / Q000032:analysis; D010949:Plasma; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013696:Temperature; D013997:Time Factors; D015262:Xenobiotics / Q000032:analysis
D010636:Phenols; D015262:Xenobiotics