An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP.
Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.
Park
Hye-Jung
HJ
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, South Korea.
Kim
Sang Kyu
SK
Park
Kyoungsook
K
Lyu
Hong-Kun
HK
Lee
Chang-Soo
CS
Chung
Sang J
SJ
Yun
Wan Soo
WS
Kim
Moonil
M
Chung
Bong Hyun
BH
D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000737:chemistry*; D008320:Maltose / Q000737:chemistry*; D058619:Maltose-Binding Proteins; D011487:Protein Conformation; D012666:Semiconductors
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D008320:Maltose