title: Weissella ghanensis sp. nov., isolated from a Ghanaian cocoa fermentation.
abstract: During a study on lactic acid bacteria (and their species diversity) in spontaneous heap fermentations of Ghanaian cocoa beans, two strains, designated 215(T) and 194B, were isolated. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that these strains represented a distinct lineage close to the genus Weissella and showing only 92.1 % 16S rRNA gene sequence similarity with respect to their closest neighbour, Weissella soli LMG 20113(T). Whole-cell protein electrophoresis, fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and physiological and biochemical tests confirmed the unique taxonomic position of the two novel isolates. On the basis of the results of the morphological and biochemical tests and 16S rRNA gene sequence analysis, strains 215(T) and 194B represent the most peripheral lineage of the genus Weissella, for which we propose the name Weissella ghanensis sp. nov. The type strain is 215(T) (=LMG 24286(T)=DSM 19935(T)).
authors.affiliation: Laboratory of Microbiology and BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: De Bruyne
authors.forename: Nicholas
authors.initials: N
authors.lastname: Camu
authors.forename: Karen
authors.initials: K
authors.lastname: Lefebvre
authors.forename: Luc
authors.initials: L
authors.lastname: De Vuyst
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D002099:Cacao / Q000378:metabolism* / Q000382:microbiology*; D005285:Fermentation; D005869:Ghana; D007777:Lactobacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D013045:Species Specificity
chemical_list: D012336:RNA, Ribosomal, 16S