Detection of Toxoplasma gondii from sera and urine of experimentally infected mice by PCR.
Toxoplasms gondii was detected in sera and urine of acutely infected mice. Animals were inoculated intraperitoneally with tachyzoites of T. gondii, RH strain. Ten animals were killed every day from day 1 up to day 7 post infection. Urine and sera of animals were collected and stored at -20 degree C until use. PCR performed by B1 gene amplification. Toxoplasma was detected in sera from 3 days post infection and in urine from 5 days post infection. No parasite was detected in control group.
Shojaee
Saeedeh
S
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Rezaie
Sasan
S
Keshavarz
Hossein
H
D000818:Animals; D006801:Humans; D051379:Mice; D025202:Molecular Diagnostic Techniques; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000235:genetics*; D014124:Toxoplasmosis, Animal / Q000097:blood* / Q000175:diagnosis / Q000652:urine*