[Sequence analysis of the DM domain of three Dmrt genes in loach, Paramisgurnus dabryanus].
We amplified genomic DNA of the Paramisgurnus dabryanus using the DM degenerate primers and detected a band, approximately 150 bp. After cloned into pMD18-T vector and sequenced, three sequences showed high homology with the DM domain. They were named as PdDmrt1, PdDmrt3, and PdDmrt5. Based on similarities of amino acid sequences of the DM domain, Dmrt gene protein sequences from six vertebrates species were included in a phylogenetic tree. It confirmed the identity of the three PCR products. Our results reveal that Dmrt gene is highly conservative in phylogeny.
Xu
Ling-Hua
LH
School of Chemical and Material Engineering, Huangshi Institute of Technology, Huangshi Hubei Province, 435003, China. linghua0317@126.com
Yan
Zhen-Jun
ZJ
Zeng
Qing-Tao
QT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D003531:Cypriniformes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*
D014157:Transcription Factors