[Novel tool for diagnosis of amyloidosis].
We synthesized new chemical compounds to detect amyloid fibrils named (trans, trans) -1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (BSB) and 1-fluoro-2,5-bis (3-carboxy-4-hydroxystyryl) benzene (FSB). We used these compounds to detect amyloid fibrils in autopsy and biopsy samples from patients with systemic amyloidosis, such as familial amyloidotic polyneuropathy, amyloid A (AA) amyloidosis, light chain (AL) amyloidosis, and dialysis-related amyloidosis, and localized amyloidosis, such as familial prion disease (Gerstmann-Straussler-Scheimker syndrome: GSS). BSB showed reactions in all Congo red positive and immunoreactive regions of the samples examined in the study, and some amyloid fibrils in the tissues could be detected more precisely with BSB and FSB than with the other methods. In the mouse model of AA amyloidosis, injected BSB reacted with amyloid in all regions in the serial sections in which Congo red staining was positive. A highly sensitive 27-MHz quartz crystal microbalance analysis revealed that BSB showed a significant affinity for amyloid fibrils purified from familial amyloidotic polyneuropathy and dialysis-related amyloidosis samples and suppressed formation of transthyretin amyloid in vitro. These results suggest that BSB and FSB may become valuable tools for detection of amyloid deposits in amyloidosis and of the mechanism of amyloid formation.
Obayashi
Konen
K
Department of Diagnostic Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-0811, Japan. konen@fc.kuh.kumamoto-u.ac.jp
Ando
Yukio
Y
D000328:Adult; D000682:Amyloid / Q000378:metabolism; D000686:Amyloidosis / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D013194:Staining and Labeling; D013343:Styrenes / Q000627:therapeutic use
C496440:(E,E)-1-fluoro-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene; C430896:1-bromo-2,5-bis(3-hydroxycarbonyl-4-hydroxy)styrylbenzene; D000682:Amyloid; D013343:Styrenes