Pre-freeze and post-thaw characteristics on chimerism patterns in double-unit cord blood transplantation.
We analyzed the pre-freeze and post-thaw characteristics on chimerism patterns in 20 cases of double-unit cord blood transplantation. The cord blood units (CBUs) were a 4/6 HLA match or better with recipients and achieved a minimum combined precryo-preservation cell dose of 3.7 x 10(7) total nucleated cell (TNC)/kg. The unit with a higher cell dose was infused first. All evaluable patients engrafted at a median of 18 days. By day 42, neutrophil engraftment was derived from both donors in 63% of cases and a single donor in 37% of patients. By day 100, one unit predominated in 80% of the patients. Higher pre-freeze TNC and CD34+ cell doses were associated with cord predominance in 67% of patients.
Jaing
Tang-Her
TH
Division of Hematology and Oncology, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan, Taiwan. jaing001@cgmh.org.tw
Tsay
Pei-Kwei
PK
Lin
Tung-Liang
TL
Yang
Chao-Ping
CP
Hung
Iou-Jih
IJ
Wen
Yu-Chuan
YC
D046528:Chimerism; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D005615:Freezing; D006402:Hematologic Diseases / Q000628:therapy; D006650:Histocompatibility Testing; D006801:Humans; D019172:Transplantation Conditioning