Synergistic degradation of 2-naphthol by Fusarium proliferatum and Bacillus subtilis in wastewater.
2-Naphthol, which originates widely from various industrial activities, is toxic and thus harmful to human liver and kidney. A new compound biodegradation system was adopted to degrade 2-naphthol-contaminated wastewater. Enzymatic response to 2-naphthol biodegradation in the aqueous phase was also studied. As a co-metabolic substrate, salicylic acid could induce the two microorganisms to produce a large amount of degradation enzymes for 2-naphthol. The key enzymes were confirmed as polyphenol oxidase (PPO) and catechol 2,3-dioxygenase (C23O). The degradation extent of 2-naphthol, determined by high performance liquid chromatography (HPLC), was enhanced by nearly 15% on the 6th day after the addition of the co-metabolic substrate. The results obtained thus clearly indicated that the co-metabolic process was the most important factor affecting the degradation of the target contaminant. The optimal concentration of 2-naphthol was 150 mg L(-1), and the optimal pH value was 7.0. The degradation extent of 2-naphthol was further enhanced by nearly 10% after the addition of Tween 80, which increased the bioavailability of 2-naphthol. In a practical treatment of industrial wastewater from medical manufacture, the synergistic degradation system resulted in a high degradation efficiency of 2-naphthol although its lag time was a little long in the initial stage.
Zang
Shuyan
S
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China.
Lian
Bin
B
D001412:Bacillus subtilis / Q000378:metabolism; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000378:metabolism; D004156:Catechol Oxidase / Q000378:metabolism; D005670:Fusarium / Q000378:metabolism; D009284:Naphthols / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods / Q000592:standards; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000592:standards; D018508:Water Purification / Q000379:methods / Q000592:standards
D009284:Naphthols; D014874:Water Pollutants, Chemical; D004156:Catechol Oxidase; D050557:Catechol 2,3-Dioxygenase; C028405:2-naphthol