title: Recovery of DNA and fingerprints from touched documents.
abstract: This study investigated the various factors affecting DNA profiling from DNA recovered from fingerprints deposited on paper before and after fingerprint enhancement treatments. The DNeasy plant mini kit (QIAGEN) was found to improve DNA recovery from paper by over 150% compared with the QIAamp mini kit. A significant decrease in the amount of DNA recovered was observed following treatment with DFO and/or Ninhydrin. This decrease in yield did not have a comparably significant effect on the quality of the SGM Plus profiles. Furthermore, this study found that whilst certain paper types, such as newspaper, magazine and filter paper allowed for the good recovery of DNA, common office paper and white card, strongly interfered with the recovery of DNA resulting in poor quality profiles.
authors.affiliation: Department of Forensic Science and Drug Monitoring, King's College London, London, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sewell
authors.forename: Ignacio
authors.initials: I
authors.lastname: Quinones
authors.forename: Carole
authors.initials: C
authors.lastname: Ames
authors.forename: Bryan
authors.initials: B
authors.lastname: Multaney
authors.forename: Stuart
authors.initials: S
authors.lastname: Curtis
authors.forename: Haj
authors.initials: H
authors.lastname: Seeboruth
authors.forename: Stephen
authors.initials: S
authors.lastname: Moore
authors.forename: Barbara
authors.initials: B
authors.lastname: Daniel
mesh_terms: D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D003676:Deferoxamine; D006801:Humans; D009555:Ninhydrin; D010209:Paper; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000737:chemistry; D014110:Touch
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA; D009555:Ninhydrin; D003676:Deferoxamine