Developmental and environmental regulation of AaeIAP1 transcript in Aedes aegypti.
Apoptosis (programmed cell death) is a tightly regulated physiological process. The inhibitors of apoptosis proteins (IAPs) are key regulators for apoptosis. An inhibitor of apoptosis protein gene IAP1 was recently cloned from Aedes aegypti (L.) (AaeIAP1, GenBank accession no. DQ993355); however, it is not clear whether AaeIAP1 is developmentally and environmentally regulated. In this study, we applied quantitative polymerase chain reaction (PCR) to investigate the expression levels of the AaeIAPI transcript in different developmental stages and under different environmental conditions. Our results revealed that the expression of the AaeIAP1 transcript was detectable in all life stages ofAe. aegypti, with significantly higher levels in pupal and adult stages than in larval stages. Furthermore, when Ae. aegypti was exposed to all stressful environmental conditions (e.g., low and high temperatures, UV radiation, acetone, and permethrin insecticide treatment), the expression level of AaeIAP1 transcript was increased significantly. Our results suggest that AaeIAP1 might play an important role in both the physiological development ofAe. aegypti and stress-induced apoptosis.
Pridgeon
Julia W
JW
Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA. julia.pridgeon@ars.usda.gov
Zhao
Liming
L
Becnel
James J
JJ
Clark
Gary G
GG
Linthicum
Kenneth J
KJ
D000330:Aedes / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D018869:Heat-Shock Response; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D008018:Life Cycle Stages; D026023:Permethrin / Q000494:pharmacology; D013696:Temperature; D014466:Ultraviolet Rays
D051034:Inhibitor of Apoptosis Proteins; D026023:Permethrin