[Cloning and bioinformatic analysis of calcineurin B gene in Setosphaeria turcica].
OBJECTIVE
To clone and identify CnB gene from Setosphaeria turcica and to perform the primary bioinformatic analysis.


METHODS
Using RACE method, we isolated the completed cDNA sequence and DNA sequence of CnB gene from S. turcica. Corresponding structure and functions were predicted by the bioinformatic software.


RESULTS
The results showed that CnB gene had 4 exons and 3 introns and the largest open reading frame was 525 bp which encoded a protein of 174 amino acid residues. The predicted secondary structure composition for the protein contained about 59.77% alpha helixes, 8.62% beta turn, 6.32% extended strand and 25.29% random coil. The CnB gene of S. turcica had 4 "EF-hand" calcium-binding regions, and showed 90% homology with CnB gene of P. nodorum, B. cinerea and Neurospora crassa.


CONCLUSIONS
The CnB gene was successfully cloned from S. turcica for the first time, providing a good foundation for further research on its function.
Yanmei
Liu
L
Mycotoxin Laboratory of Agricultural University of Hebei, Baoding 071001, China. lym0807@tom.com
Jianmin
Han
H
Jingao
Dong
D
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D019703:Calcineurin / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D019295:Computational Biology; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny
D005656:Fungal Proteins; D019703:Calcineurin