Mobilization of qnrB2 and ISCR1 in plasmids.
The DNA sequences of two IncHI2 plasmids, pEC-IMP and pEC-IMPQ, from metallo-beta-lactamase-producing Enterobacter cloacae clinical isolates were determined. The two conjugative plasmids are almost identical, but pEC-IMPQ carries an additional segment containing an orf513 (ISCR1), a truncated 3' conserved sequence, and a qnrB2. Comparative analyses provide support for the proposed ISCR1-mediated gene mobilization.
Chen
Ying-Tsong
YT
Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli, Taiwan.
Liao
Tsai-Lien
TL
Liu
Yen-Ming
YM
Lauderdale
Tsai-Ling
TL
Yan
Jing-Jou
JJ
Tsai
Shih-Feng
SF
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D015363:Quinolones; D001618:beta-Lactamases