Recovery of DNA and fingerprints from touched documents.
This study investigated the various factors affecting DNA profiling from DNA recovered from fingerprints deposited on paper before and after fingerprint enhancement treatments. The DNeasy plant mini kit (QIAGEN) was found to improve DNA recovery from paper by over 150% compared with the QIAamp mini kit. A significant decrease in the amount of DNA recovered was observed following treatment with DFO and/or Ninhydrin. This decrease in yield did not have a comparably significant effect on the quality of the SGM Plus profiles. Furthermore, this study found that whilst certain paper types, such as newspaper, magazine and filter paper allowed for the good recovery of DNA, common office paper and white card, strongly interfered with the recovery of DNA resulting in poor quality profiles.
Sewell
Jonathan
J
Department of Forensic Science and Drug Monitoring, King's College London, London, UK.
Quinones
Ignacio
I
Ames
Carole
C
Multaney
Bryan
B
Curtis
Stuart
S
Seeboruth
Haj
H
Moore
Stephen
S
Daniel
Barbara
B
D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D003676:Deferoxamine; D006801:Humans; D009555:Ninhydrin; D010209:Paper; D011933:Reagent Kits, Diagnostic; D012463:Saliva / Q000737:chemistry; D014110:Touch
D011933:Reagent Kits, Diagnostic; D004247:DNA; D009555:Ninhydrin; D003676:Deferoxamine