Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus.
Alveolar macrophages from PRRSV-infected and naïve pigs were placed into culture and infected with PRRSV laboratory strain SD-23983. Permissiveness increased with time in culture, and macrophages from infected pigs could be superinfected. Addition of actinomycin D, an inhibitor of mRNA synthesis, blocked infection. Interferon-gamma reduced infection in cultures, while the addition of tumor necrosis factor-alpha or interleukin (IL)-10 did not affect permissiveness. IL-4 produced a marginal increase in the percentage of infected cells, but without a detectable increase in virus yield. These results suggest that the PRRSV-permissive population of cells in culture arises from a non-permissive precursor population and depends on new mRNA synthesis.
Gaudreault
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Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, USA.
Rowland
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RR
Wyatt
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CR
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000821:virology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D034421:Sus scrofa; D013997:Time Factors
D000998:Antiviral Agents; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D016753:Interleukin-10; D003609:Dactinomycin; D007371:Interferon-gamma