Protocols for microapplicator-assisted infection of lepidopteran larvae with baculovirus.
Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.
Li
Huarong
H
Department of Entomology, Iowa State University, USA. hrli@iastate.edu
Sparks
Wendy
W
Bonning
Bryony
B
D000818:Animals; D016367:Baculoviridae / Q000254:growth & development*; D006458:Hemolymph / Q000821:virology; D007915:Lepidoptera / Q000821:virology*; D008018:Life Cycle Stages; D014773:Virology / Q000379:methods*