[T lymphocyte subgroups and serum levels of trace elements in patients with diarrhea type of irritable bowel syndrome].
To investigate the T lymphocyte subgroups and the levels of serum zinc (Zn), selenium (Se), iron (Fe), copper (Cu) in patients with diarrhea type of irritable bowel syndrome (D-IBS).



A total of 30 D-IBS patients and 30 control subjects were enrolled in this study, and their peripheral blood samples were collected. The percentage of peripheral CD3, CD4, CD8 T lymphocytes were analyzed by flow cytometry, and the ratio of CD4/CD8 was calculated. Serum Zn, Fe and Cu levels were determined by atomic absorption spectrometry(AAS), and the Se level by atomic fluorometry.



Compared with control group,the percentage of CD4 T lymphocyte and the ratio of CD4/CD8 in D-IBS group were significantly lower (P<0.01). However, there was no significant difference in serum Zn, Se, Fe, Cu levels between two groups (P>0.05).



The declines of peripheral blood CD4 T lymphocytes and the ratio of CD4/CD8 may suggest a cellular immune abnormality in D-IBS patients. There was no significant difference in trace elements levels between the two groups.
Jin
Hang-bin
HB
Department of Gastroenterology, The First Affiliated Hospital, College of Medical, Zhejiang University, Hangzhou 31003, China.
Gu
Zhu-ying
ZY
Zhao
Hong-wen
HW
Wang
Qun-yan
QY
Wu
Wei
W
Li
You-ming
YM
D000328:Adult; D016516:CD4-CD8 Ratio; D016022:Case-Control Studies; D003300:Copper / Q000097:blood; D003967:Diarrhea / Q000209:etiology; D005260:Female; D006801:Humans; D007501:Iron / Q000097:blood; D043183:Irritable Bowel Syndrome / Q000097:blood* / Q000150:complications / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012643:Selenium / Q000097:blood; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014131:Trace Elements / Q000097:blood*; D015032:Zinc / Q000097:blood
D014131:Trace Elements; D003300:Copper; D007501:Iron; D012643:Selenium; D015032:Zinc