Homeodomain transcription factor CDX1 is required for the transcriptional induction of PPARgamma in intestinal cell differentiation.
The caudal-associated homeobox transcription factor, CDX1, performs critical functions in intestinal cell proliferation, differentiation, and neoplasia. In this study, we have identified the molecular mechanism underlying the CDX1-mediated regulation of intestinal cell differentiation. CDX1 enhanced the expression of PPARgamma gene, a master transcriptional regulator of intestinal differentiation, at the transcriptional level, via functional interaction with CCAAT/enhancer-binding protein alpha (C/EBPalpha). Butyrate, an intestinal differentiation inducer, increased the protein interaction of CDX1 and C/EBPalpha, thereby resulting in a significant increase in PPARgamma expression. These results show that the regulation of target gene expression for intestinal determination is dependent on a transcriptional cascade of CDX1, C/EBPalpha, and PPARgamma.
Park
Min Jung
MJ
Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan, Republic of Korea.
Kim
Hye Young
HY
Kim
KyeongJin
K
Cheong
JaeHun
J
D002087:Butyrates / Q000494:pharmacology; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D003106:Colon / Q000166:cytology* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047495:PPAR gamma / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000235:genetics; D015533:Transcriptional Activation
D002087:Butyrates; D022763:CCAAT-Enhancer-Binding Protein-alpha; C491273:CDX1 protein, human; D018398:Homeodomain Proteins; D047495:PPAR gamma