title: Roundup Ready soybean gene concentrations in field soil aggregate size classes.
abstract: Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of genetic transformation of soil bacteria. This study used real-time PCR to examine the concentration of cp4 epsps in four field soil aggregate size classes (>2000 microm, 2000-500 microm, 500-250 microm and <250 microm). Aggregates over 2000 microm in diameter had significantly greater gene concentrations than those with diameters under 2000 microm. The >2000 mum fraction contained between 66.62% and 99.18% of total gene copies, although it only accounted for about 30.00% of the sampled soil. Aggregate formation may facilitate persistence of recombinant DNA.
authors.affiliation: Department of Environmental Biology, University of Guelph, Guelph, ON, Canada.
authors.forename: David J
authors.initials: DJ
authors.lastname: Levy-Booth
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gulden
authors.forename: Rachel G
authors.initials: RG
authors.lastname: Campbell
authors.forename: Jeff R
authors.initials: JR
authors.lastname: Powell
authors.forename: John N
authors.initials: JN
authors.lastname: Klironomos
authors.forename: K Peter
authors.initials: KP
authors.lastname: Pauls
authors.forename: Clarence J
authors.initials: CJ
authors.lastname: Swanton
authors.forename: Jack T
authors.initials: JT
authors.lastname: Trevors
authors.forename: Kari E
authors.initials: KE
authors.lastname: Dunfield
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000032:analysis* / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D037102:Lectins / Q000032:analysis / Q000235:genetics; D010316:Particle Size; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D012231:Rhizobium / Q000201:enzymology; D012987:Soil / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D018744:DNA, Plant; D037102:Lectins; D010940:Plant Proteins; D012987:Soil; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase