title: Pre-freeze and post-thaw characteristics on chimerism patterns in double-unit cord blood transplantation.
abstract: We analyzed the pre-freeze and post-thaw characteristics on chimerism patterns in 20 cases of double-unit cord blood transplantation. The cord blood units (CBUs) were a 4/6 HLA match or better with recipients and achieved a minimum combined precryo-preservation cell dose of 3.7 x 10(7) total nucleated cell (TNC)/kg. The unit with a higher cell dose was infused first. All evaluable patients engrafted at a median of 18 days. By day 42, neutrophil engraftment was derived from both donors in 63% of cases and a single donor in 37% of patients. By day 100, one unit predominated in 80% of the patients. Higher pre-freeze TNC and CD34+ cell doses were associated with cord predominance in 67% of patients.
authors.affiliation: Division of Hematology and Oncology, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan, Taiwan. jaing001@cgmh.org.tw
authors.forename: Tang-Her
authors.initials: TH
authors.lastname: Jaing
authors.forename: Pei-Kwei
authors.initials: PK
authors.lastname: Tsay
authors.forename: Tung-Liang
authors.initials: TL
authors.lastname: Lin
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Yang
authors.forename: Iou-Jih
authors.initials: IJ
authors.lastname: Hung
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Wen
mesh_terms: D046528:Chimerism; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology*; D005615:Freezing; D006402:Hematologic Diseases / Q000628:therapy; D006650:Histocompatibility Testing; D006801:Humans; D019172:Transplantation Conditioning