Bone marrow-derived dendritic cells pulsed with tumor lysates induce anti-tumor immunity against gastric cancer ex vivo.
OBJECTIVE
To investigate whether bone marrow-derived denritic cells pulsed with tumor lysates induce immunity against gastric cancer ex vivo.


METHODS
c-kit(+) hematopoietic progenitor cells were magnetically isolated with a MiniMACS separator from BALB/c mice bone marrow cells. These cells were cultured with cytokines GM-CSF, IL-4, and TNFalpha to induce their maturation. They were analysed by morphological observation, phenotype analysis, and mixed lymphocyte reaction (MLR). Bone marrow-derived DCs (BM-DCs) were pulsed with tumor cell lysate obtained by rapid freezing and thawing at a 1:3 DC:tumor cell ratio. Finally, cytotoxic T lymphocyte (CTL) activity and interferon gamma (IFNgamma) secretion was evaluated ex vivo.


RESULTS
c-kit(+) hematopoietic progenitor cells from mice bone marrow cells cultured with cytokines for 8 d showed the character of typical mature DCs. Morphologically, observed by light microscope, these cells were large with oval or irregularly shaped nuclei and with many small dendrites. Phenotypically, FACS analysis showed that they expressed high levels of Ia, DEC-205, CD11b, CD80 and CD86 antigen, moderate levels of CD40, and negative for F4/80. Functionally, these cells gained the capacity to stimulate allogeneic T cells in MLR assay. However, immature DCs cultured with cytokines for 5 d did not have typical DCs phenotypic markers and could not stimulate allogeneic T cells. Ex vivo primed T cells with SGC-7901 tumor cell lysate-pulsed (TP) DCs were able to induce effective CTL activity against SGC-7901 tumor cells (E:T = 100:1, 69.55% +/- 6.05% specific lysis), but not B16 tumor cells, and produced higher levels of IFNgamma when stimulated with SGC-7901 tumor cells but not when stimulated with B16 tumor cells (1575.31 +/- 60.25 pg/mL in SGC-7901 group vs 164.11 +/- 18.52 pg/mL in B16 group, P < 0.01).


CONCLUSIONS
BM-derived DCs pulsed with tumor lysates can induce anti-tumor immunity specific to gastric cancer ex vivo.
Li
Yan-Lin
YL
Department of Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
Wu
Yu-Gang
YG
Wang
Yong-Qing
YQ
Li
Zhong
Z
Wang
Rong-Chao
RC
Wang
Liang
L
Zhang
Yan-Yun
YY
D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D019496:Cancer Vaccines / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000473:pathology*; D004195:Disease Models, Animal; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013274:Stomach Neoplasms / Q000473:pathology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology
D019496:Cancer Vaccines; D007371:Interferon-gamma