title: Infrared laser-mediated gene induction in targeted single cells in vivo.
abstract: We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.
authors.affiliation: Radiation Biology Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto, Japan. ykamei@radbio.med.osaka-u.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kamei
authors.forename: Motoshi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fujimori
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Tomonori
authors.initials: T
authors.lastname: Deguchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoneda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Todo
authors.forename: Shin
authors.initials: S
authors.lastname: Takagi
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yuba
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000528:radiation effects*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D002138:Calibration; D004926:Escherichia coli / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*
chemical_list: D029742:Caenorhabditis elegans Proteins