title: Method for conducting microarray study of oxidative stress induced gene expression.
abstract: Cellular systems produce reactive oxygen species during the process of metabolism. Oxidative stress results in the activation or repression of many genes in important signaling pathways. DNA microarrays allow for a high throughput evaluation of the changes in gene expression levels in any biological system. In this study, we describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria.
authors.affiliation: Center for Immunology & Microbial Disease, Albany College of Medicine, Albany, NY, USA.
authors.forename: Sita
authors.initials: S
authors.lastname: Subbaram
authors.forename: Juan A
authors.initials: JA
authors.lastname: Melendez
authors.forename: Sridar V
authors.initials: SV
authors.lastname: Chittur
mesh_terms: D045744:Cell Line, Tumor; D004586:Electrophoresis; D005434:Flow Cytometry; D005452:Fluoresceins; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016877:Oxidants / Q000032:analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018075:RNA, Complementary / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D005452:Fluoresceins; D016877:Oxidants; D018075:RNA, Complementary; D012333:RNA, Messenger; C029569:diacetyldichlorofluorescein