Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control.
The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.
Xiao
Xing-Long
XL
Department of Food, Institution of Food safety, College of Light Industry and Food, South China University of Technology, Guangzhou, China.
He
Ya-Qing
YQ
Yu
Yi-Gang
YG
Yang
Hong
H
Chen
Gu
G
Li
Hui-Fang
HF
Zhang
Jing-Wei
JW
Liu
Dong-Mei
DM
Li
Xiao-Feng
XF
Yang
Xiao-Quan
XQ
Wu
Hui
H
D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000821:virology; D004770:Enterovirus / Q000302:isolation & purification / Q000502:physiology*; D029821:Enterovirus A, Human / Q000302:isolation & purification / Q000502:physiology*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D012367:RNA, Viral