Insight into Anopheles (Nyssorhynchus) (Diptera: Culicidae) species from Brazil.
Anopheles (Nyssorhynchus) benarrochi s.l., Anopheles (Nyssorhynchus) oswaldoi s.l., and Anopheles (Nyssorhynchus) konderi s.l. collected in Acrelandia, state of Acre, Brazil, were identified based on morphological characters of the male genitalia, fourth-instar larvae, and pupae. Morphological variation was observed in the male genitalia of these species in comparison with specimens from other localities in Brazil. DNA sequence from the nuclear ribosomal second internal transcribed spacer of individuals identified as An. benarrochi s.l. by using male genitalia characteristics showed that the various morphological forms are conspecific but are distinct from An. benarrochi B from Colombia. Anopheles konderi s.l. and An. oswaldoi s.l. both misidentified as An. oswaldoi s.s. (Peryassti) throughout Brazil, may actually comprise at least two undescribed species. Diagnostic morphological characteristics of the male genitalia are provided to distinguish Anopheles benarrochi s.l., Anopheles oswaldoi s.l., and Anopheles konderi s.l. from morphologically similar species. Incrimination of An. oswaldoi s.s. in malaria transmission in Brazil needs further investigation because other undescribed species from Acre may have been confounded with this taxon.
Sallum
M A M
MA
Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de SSo Paulo, Avenida Doutor Arnaldo 715, CEP 01246-904, São Paulo, Brazil. masallum@usp.br
Marrelli
M T
MT
Nagaki
S S
SS
Laporta
G Z
GZ
Dos Santos
C L S
CL
D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D001938:Brazil; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005835:Genitalia / Q000033:anatomy & histology; D008297:Male; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer