[Study on expression of CTGF and WISP-1 genes in human lung cancers].
OBJECTIVE
To investigate CTGF and WISP-1 genes expression levels and theirs relations to clinical and pathological features in human lung carcinomas.


METHODS
The CTGF and WISP-1 mRNA expression levels in samples from sixty primary lung cancers and their matched normal lung tissues were quantified by quantitative real-time PCR assay and immunohistochemistry staining.


RESULTS
Down-regulations of CTGF gene were quantified found in 65% (39/60) primary lung cancers in comparison the paired normal lung tissues (t = -1.59, P = 0.016). The up-regualtions of WISP-1 gene were observed in 83% (50/60) lung cancers in comparison their normal counterparts (t = 4.15, P = 0.000). These results were further conformed by immunohistochemistry staining. Pearson's correlation analysis showed that WISP-1 was negatively associated with CTGF (r = - 0.299, P = 0.020). Multiple linear regression analysis revealed that the position of the tumor and sex were key factors for CTGF expression, and tumor type, age, family history were valuable predictors for WISP-1 expression.


CONCLUSIONS
These results suggested that CTGF and WISP-1 could play an important role in the progression of primary lung cancers by either individual gene itself or two-gene co-interactions of these genes and theirs relations to clinical and pathological features.
Li
Ning
N
Department of Nutrition and Food Hygiene, College of Public Health,Zhengzhou University, Zhengzhou 450001, China. lining8028@yahoo.com.cn
Wang
Qi
Q
Chen
Pingping
P
Xie
Dong
D
D055511:CCN Intercellular Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012044:Regression Analysis
D055511:CCN Intercellular Signaling Proteins; C526317:CCN2 protein, human; C116396:CCN4 protein, human; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor