title: Counting and determining the viability of cultured cells.
abstract: Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.
authors.affiliation: Molecular Pathology Laboratory Network, Inc., USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Ricardo
authors.forename: Katy
authors.initials: K
authors.lastname: Phelan
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D006801:Humans; D014343:Trypan Blue / Q000737:chemistry
chemical_list: D014343:Trypan Blue