Determination of bacteriophage genome size by pulsed-field gel electrophoresis.
Standard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40-50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.
Lingohr
Erika
E
Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
Frost
Shelley
S
Johnson
Roger P
RP
D001435:Bacteriophages / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016679:Genome, Viral / Q000235:genetics*