[Preparation and in vitro targeting of follicle stimulating hormone polypeptide modified nanoparticles].
OBJECTIVE
To prepare follicle stimulating hormone (FSH) polypeptide modified nanoparticles (NP) in order to achieve specific ovarian tumor targeting.


METHODS
Expression of FSH receptor protein in human liver cancer and ovarian cancer cell lines BEL-7402, SKOV-3 and Caov-3 was detected by immunocytochemistry. The polypeptide fragment of FSH beta 81-95 amino acids (FSHL81-95) was synthesized and covalently coupled to NP. The specific binding of FSHL81-95 and FSHL81-95-NP was examined by fluorescence microscopy and flow cytometry.


RESULTS
BEL-7402 and SKOV-3 cells were negative for FSH receptor staining, while Caov-3 cells were positive. The diameters of NP were about 100 nm, with a Zeta potential of -25 mV or so. Caov-3 cells showed a more specific interaction with FSHL81-95-NP than SKOV-3 cells (4.17 +/- 0.86 and 2.30 +/- 0.21; P < 0.05). The uptake of FSHL81-95-NP was more than NP in Caov-3 cells (4.17 +/- 0.86 and 0.41 +/- 0.32; P < 0.05). FSHL81-95-NP showed a selective targeting at Caov-3 cells compared with control NP.


CONCLUSIONS
FSH polypeptide modified NP could selectively target ovarian cancer cells expressing FSH receptor, which might contribute to specific endocytosis mediated by FSH receptor.
Zhang
Xiao-yan
XY
Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
Chen
Jun
J
Gao
Xiao-ling
XL
Sun
Hong
H
Xu
Cong-jian
CJ
D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D043373:Follicle Stimulating Hormone, Human / Q000008:administration & dosage / Q000494:pharmacology; D037201:Follicle Stimulating Hormone, beta Subunit / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D053758:Nanoparticles; D010051:Ovarian Neoplasms / Q000473:pathology*; D010316:Particle Size; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D011962:Receptors, FSH / Q000378:metabolism*
D043373:Follicle Stimulating Hormone, Human; D037201:Follicle Stimulating Hormone, beta Subunit; D010455:Peptides; D011962:Receptors, FSH