Monitoring of Brucella reactor does following milk examination using different techniques.
Milk samples from 129 does were collected and monitored for Brucella antibodies using immunological tests such as Milk Ring Test (MRT), Whey Agglutination Test (WAT), Whey Antiglobulin Coombs Test (WCT) and milk ELISA (m ELISA) using Brucella Periplasmic protein antigen. Results obtained from these tests were compared to PCR and bacterial isolation. The highest incidence of positive reactors was given by Whey Antiglobulin and Whey Agglutination Test (9.3%) while the lowest incidence was given by bacterial isolation (Br. melitensis biovars 3, 3.8%). PCR showed the highest agreement with the bacterial isolation, while WAT and WCT showed the lowest one. PCR showed a high sensitivity of 1 x 10 B. melitensis CFU mL(-1) of milk. The results of mELISA here suggests its efficiency to be used as a screening test and/or confirmatory test, while the modified MRT still need more investigations to diagnosis caprine brucellosis.
el-Razik
K A Abd
KA
Department of Animal Reproduction and A.I. National Research Center, Dokki, Giza, Egypt.
Ghazi
Y A
YA
Salama
E M
EM
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002002:Brucella / Q000235:genetics* / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006041:Goats; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary
D000907:Antibodies, Bacterial