Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 using response surface methodology.
Sequential methodology combining a screening stage by fractional factorial design and an optimization stage by central composite design was applied to enhance canthaxanthin production of Dietzia natronolimnaea HS-1 in shake flask cultures. Five variables (pH, luminous intensity, inoculum percent, concentration of glucose and concentration of NaCl) were studied with the first design and the results revealed that three factors (pH, concentration of glucose and concentration of NaCl) had greater influence on the canthaxanthin production (p<0.01). A central composite design was then used in the second step to determine the maximum canthaxanthin concentration. The optimum condition for the highest canthaxanthin production (5.32 mg L(-1)) was a pH of 7.53, glucose concentration of 25.90 g L(-1) and NaCI concentration of 3.42 g L(-1).
Khodaiyan
Faramarz
F
Department of Food Science and Engineering, Faculty of Biosystem Engineering, University of Tehran, Iran
Razavi
Seyed Hadi
SH
Emam-Djomeh
Zahra
Z
Mousavi
Seyed Mohammad Ali
SM
D000192:Actinomycetales / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D018533:Biomass; D016644:Canthaxanthin / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques; D013696:Temperature
D000975:Antioxidants; D016644:Canthaxanthin; D002244:Carbon