Down-regulation of CD40 gene expression and inhibition of apoptosis with Danshensu in endothelial cells.
Danshen is commonly used in China for the treatment of atherosclerosis-related disorders such as cardiovascular and cerebrovascular diseases. Research shows that it also has immunostimulation properties. The present study evaluates the protective effect of danshensu, an active water-extractable component isolated from danshen, on an endothelial cell line (CRL-1730) treated with hydrogen peroxide (H(2)O(2)). Danshensu significantly inhibited endothelial cell viability induced by H(2)O(2). The treatment of endothelial cells with danshensu resulted in most cells being arrested in the S and G(2)/M phases of the cell cycle. The fraction of cells in G(0)/G(1) phase was markedly decreased by danshensu treatment compared to the control groups. The apoptosis was also markedly decreased after danshensu treatment. Additionally, danshensu restrains decreased nitric oxide level, increased the release of lactate dehydrogenase and expression of cluster of differentiation 40 (CD40) significantly. These results suggest that danshensu protects endothelial cells from the damage induced by H(2)O(2) through its CD40 anti-inflammatory approach and cell apoptosis inhibition.
Yang
Guang-De
GD
School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Zhang
Hui
H
Lin
Rong
R
Wang
Wei-Rong
WR
Shi
Xiao-Lian
XL
Liu
Yu
Y
Ji
Qiao-Li
QL
D017209:Apoptosis / Q000187:drug effects*; D019013:CD40 Antigens / Q000235:genetics*; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry
D019013:CD40 Antigens; D004365:Drugs, Chinese Herbal; D007773:Lactates; D009569:Nitric Oxide; C035055:3,4-dihydroxyphenyllactic acid; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase