title: mKikGR, a monomeric photoswitchable fluorescent protein.
abstract: The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Habuchi
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tsutsui
authors.forename: Anna B
authors.initials: AB
authors.lastname: Kochaniak
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Antoine M
authors.initials: AM
authors.lastname: van Oijen
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D038761:Photobleaching / Q000528:radiation effects; D013050:Spectrometry, Fluorescence
chemical_list: D008164:Luminescent Proteins; C535245:mKikGR protein