Approaches to the compositional analysis of DNA.
DNA base compositional analysis is something which is rarely undertaken today, but it is still a useful criterion for phage taxonomy. A variety of techniques are described including hydrolysis of the DNA to the level of bases or nucleosides and separation by paper chromatography or HPLC. Spectroscopic and spectrofluorometric procedures are also outlined.
Manderville
Richard A
RA
Department of Chemistry, University of Guelph, Guelph, Ontario, Canada.
Kropinski
Andrew M
AM
D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D009705:Nucleosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
D004279:DNA, Viral; D009705:Nucleosides