title: Halomonas daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield soil.
abstract: A Gram-negative, moderately halophilic, short rod-shaped, aerobic bacterium with peritrichous flagellae, strain DQD2-30(T), was isolated from a soil sample contaminated with crude oil from the Daqing oilfield in Heilongjiang Province, north-eastern China. The novel strain was capable of growth at NaCl concentrations of 1-15 % (w/v) [optimum at 5-10 % (w/v)]. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Halomonas in the class Gammaproteobacteria; the highest 16S rRNA gene sequence similarities were with Halomonas desiderata DSM 9502(T) (98.8 %), Halomonas campisalis A4(T) (96.6 %) and Halomonas gudaonensis CGMCC 1.6133(T) (95.1 %). The major cellular fatty acids of strain DQD2-30(T) were C(18 : 1)omega7c (43.97 %), C(19 : 0 )cyclo omega8c (23.37 %) and C(16 : 0) (14.83 %). The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q9). The DNA G+C content was 67.0 mol%. The DNA-DNA hybridization values of strain DQD2-30(T) with the most closely related species of the genus Halomonas were 51.8 %, 28.4 % and 23.5 % for H. desiderata, H. campisalis and H. gudaonensis, respectively. Based on these analyses, strain DQD2-30(T )(=CGMCC 1.6443(T)=LMG 23896(T)) is proposed to represent the type strain of a novel species, Halomonas daqingensis sp. nov.
authors.affiliation: State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Wu
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Chang-Qiao
authors.initials: CQ
authors.lastname: Chi
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Kenji
authors.initials: K
authors.lastname: Kida
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
authors.forename: Zhao-Kun
authors.initials: ZK
authors.lastname: Luan
mesh_terms: D005227:Fatty Acids / Q000032:analysis; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S