title: General M13 phage display: M13 phage display in identification and characterization of protein-protein interactions.
abstract: In M13 phage display, proteins and peptides are exposed on one of the surface proteins of filamentous phage particles and become accessible to affinity enrichment against a bait of interest. We describe the construction of fragmented whole genome and gene fragment phage display libraries and interaction selection by panning. This strategy allows the identification and characterization of interacting proteins on a genomic scale by screening the fragmented "proteome" against protein baits. Gene fragment libraries allow a more in depth characterization of the protein-protein interaction site by identification of the protein region involved in the interaction.
authors.affiliation: Department of Biosystems, Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hertveldt
authors.forename: Tim
authors.initials: T
authors.lastname: Beliën
authors.forename: Guido
authors.initials: G
authors.lastname: Volckaert
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics* / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D019151:Peptide Library