title: Complement activation in tear fluid during occupational mold challenge.
abstract: BACKGROUND
  Indoor air quality is important in occupational healthcare when evaluating the health risks of a work environment. Components of the classical and alternative complement pathways are present in ocular tissues and fluids. The authors determined the levels of complement components C1INH, C3, and C4 in sera and C3a in tear fluids of normal persons and of those who were exposed to molds.
  
  
  METHODS
  Nine patients environmentally exposed to molds and 6 controls were selected from the Indoor Air Clinic of the Skin and Allergy Hospital. Tear fluid samples were collected from patients during the exposure to molds and after 2 weeks without mold exposure. At the same time, conjunctival cytology samples were obtained from each patient. Tear fluid was taken from 6 control subjects. All had negative skin prick tests to common environmental allergens.
  
  
  RESULTS
  In 4 patients subjective eye symptoms and tear fluid C3a levels decreased during 2 weeks of sick leave as did conjunctival eosinophils but other inflammatory cells were unchanged.
  
  
  CONCLUSIONS
  Elevated complement C3a levels in tear fluids may be influenced by environmental exposure to molds. According to the authors' clinical experience, eosinophilia is not a consistent finding in patients exposed to molds. However, molds may cause eosinophilic inflammation in the eye.
authors.affiliation: Skin and Allergy Hospital, Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Peltonen
authors.forename: O
authors.initials: O
authors.lastname: Kari
authors.forename: H
authors.initials: H
authors.lastname: Jarva
authors.forename: H
authors.initials: H
authors.lastname: Mussalo-Rauhamaa
authors.forename: T
authors.initials: T
authors.lastname: Haahtela
authors.forename: S
authors.initials: S
authors.lastname: Meri
mesh_terms: D000328:Adult; D002452:Cell Count; D003167:Complement Activation / Q000276:immunology*; D003174:Complement C1 Inactivator Proteins / Q000378:metabolism; D050718:Complement C1 Inhibitor Protein; D003176:Complement C3 / Q000378:metabolism; D015926:Complement C3a / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D003228:Conjunctiva / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D005260:Female; D005658:Fungi / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D013666:Tears / Q000276:immunology* / Q000378:metabolism
chemical_list: D003174:Complement C1 Inactivator Proteins; D050718:Complement C1 Inhibitor Protein; D003176:Complement C3; D003181:Complement C4; C469952:SERPING1 protein, human; D015926:Complement C3a