title: A functional putative phytochelatin synthase from the primitive red alga Cyanidioschyzon merolae.
abstract: Phytochelatin synthase (PCS) catalyzes the synthesis of phytochelatins (PCs), which play a detoxification role in higher plants. Heterologous expression of CmPCS, a product of a PCS-like gene from the genomic DNA of the red alga Cyanidioschyzon merolae, rescued Cd(2+)-sensitive yeast from Cd(2+) toxicity. The fact that these transformed cells synthesized PCs demonstrates that CmPCS is functional.
authors.affiliation: Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Osaki
authors.forename: Tomoo
authors.initials: T
authors.lastname: Shirabe
authors.forename: Shun
authors.initials: S
authors.lastname: Tamura
authors.forename: Etsuro
authors.initials: E
authors.lastname: Yoshimura
mesh_terms: D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000737:chemistry / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity; D015870:Gene Expression; D008969:Molecular Sequence Data; D054811:Phytochelatins / Q000096:biosynthesis; D000461:Rhodophyta / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D002104:Cadmium; D054811:Phytochelatins; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase