title: The osteogenic differentiation of adipose tissue-derived precursor cells in a 3D scaffold/matrix environment.
abstract: This paper details an in-vitro study using human adipose tissue-derived precursor/stem cells (ADSCs) in three-dimensional (3D) tissue culture systems. ADSCs from 3 donors were seeded onto NaOH-treated medical grade polycaprolactone-tricalcium phosphate (mPCL-TCP) scaffolds with two different matrix components; fibrin glue and lyophilized collagen. ADSCs within these scaffolds were then induced to differentiate along the osteogenic lineage for a 28-day period and various assays and imaging techniques were performed at Day 1, 7, 14, 21 and 28 to assess and compare the ADSC's adhesion, viability, proliferation, metabolism and differentiation along the osteogenic lineage when cultured in the different scaffold/matrix systems. The ADSC cells were proliferative in both collagen and fibrin mPCL-TCP scaffold systems with a consistently higher cell number (by comparing DNA amounts) in the induced group over the non-induced groups for both scaffold systems. In response to osteogenic induction, these ADSCs expressed elevated osteocalcin, alkaline phosphatase and osteonectin levels. Cells were able to proliferate within the pores of the scaffolds and form dense cellular networks after 28 days of culture and induction. The successful cultivation of osteogenic ADSCs within a 3D matrix comprising fibrin glue or collagen, immobilized within a robust synthetic scaffold is a promising technique which should enhance their potential usage in the regenerative medicine arena, such as bone tissue engineering.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
authors.forename: David Tai
authors.initials: DT
authors.lastname: Leong
authors.forename: Wee Keong
authors.initials: WK
authors.lastname: Nah
authors.forename: Anurag
authors.initials: A
authors.lastname: Gupta
authors.forename: Dietmar Werner
authors.initials: DW
authors.lastname: Hutmacher
authors.forename: Maria Ann
authors.initials: MA
authors.lastname: Woodruff
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology*; D000328:Adult; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D003094:Collagen; D005109:Extracellular Matrix; D005260:Female; D015718:Fibrin Tissue Adhesive; D006801:Humans; D009924:Organ Culture Techniques; D010012:Osteogenesis / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D015718:Fibrin Tissue Adhesive; D003094:Collagen