Wave expansion of CD34+ progenitor cells in the spleen in rodent malaria.
Defense against malaria depends upon amplification of the spleen structure and function for the clearance of parasitized red blood cells (pRBC). We studied the distribution and amount of CD34(+) cells in the spleens of mice infected with rodent malaria. We sought to identify these cells in the spleen and determine their relationship to infection. C57BL/6J mice were infected with self-resolving, Plasmodium chabaudi CR, or one of the lethal rodent malaria strains, P. chabaudi AJ and P. berghei ANKA. We then recorded parasitemia, mortality, and the presence of CD34(+) cells in spleen, as determined by immunohistochemistry and flow cytometry. In the non-lethal strain, the spleen structure was maintained during amplification, but disrupted in lethal models. The abundance of CD34(+) cells increased in the red pulp on the 4th and 6th days p.i. in all models, and subsided on the 8th day p.i. Faint CD34(+) staining on the 8th day p.i., was probably due to differentiation of committed cell lineages. In this work, increase of spleen CD34(+) cells did not correlate with infection control.
Hermida
Felipe Pessoa de Melo
FP
Instituto de Ciências Biomédicas da Universidade de São Paulo Professor Lineu Prestes Avenue, Cidade Universitária, São Paulo, SP 05508-900, Brazil.
Vieira
Daniel Perez
DP
Fernandes
Elaine Raniero
ER
de Andrade
Heitor Franco
HF
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018512:Parasitemia / Q000276:immunology; D010962:Plasmodium berghei / Q000276:immunology*; D016788:Plasmodium chabaudi / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
D018952:Antigens, CD34