Infrared laser-mediated gene induction in targeted single cells in vivo.
We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.
Kamei
Yasuhiro
Y
Radiation Biology Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto, Japan. ykamei@radbio.med.osaka-u.ac.jp
Suzuki
Motoshi
M
Watanabe
Kenjiro
K
Fujimori
Kazuhiro
K
Kawasaki
Takashi
T
Deguchi
Tomonori
T
Yoneda
Yoshihiro
Y
Todo
Takeshi
T
Takagi
Shin
S
Funatsu
Takashi
T
Yuba
Shunsuke
S
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000528:radiation effects*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D002138:Calibration; D004926:Escherichia coli / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*
D029742:Caenorhabditis elegans Proteins