title: Anthocyanin/polyphenolic-rich fruit juice reduces oxidative cell damage in an intervention study with patients on hemodialysis.
abstract: Hemodialysis patients face an elevated risk of cancer, arteriosclerosis, and other diseases, ascribed in part to increased oxidative stress. Red fruit juice with high anthocyanin/polyphenol content had been shown to reduce oxidative damage in healthy probands. To test its preventive potential in hemodialysis patients, 21 subjects in a pilot intervention study consumed 200 mL/day of red fruit juice (3-week run-in; 4-week juice uptake; 3-week wash-out). Weekly blood sampling was done to monitor DNA damage (comet assay +/- formamidopyrimidine-DNA glycosylase enzyme), glutathione, malondialdehyde, protein carbonyls, trolox equivalent antioxidant capacity, triglycerides, and DNA binding capacity of the transcription factor nuclear factor-kappaB. Results show a significant decrease of DNA oxidation damage (P < 0.0001), protein and lipid peroxidation (P < 0.0001 and P < 0.001, respectively), and nuclear factor-kappaB binding activity (P < 0.01), and an increase of glutathione level and status (both P < 0.0001) during juice uptake. We attribute this reduction in oxidative (cell) damage in hemodialysis patients to the especially high anthocyanin/polyphenol content of the juice. This provides promising perspectives into the prevention of chronic diseases such as cancer and cardiovascular disease in population subgroups exposed to enhanced oxidative stress like hemodialysis patients.
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Erwin-Schroedinger-Strasse 52, 67663 Kaiserslautern, Germany.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Spormann
authors.forename: Franz W
authors.initials: FW
authors.lastname: Albert
authors.forename: Thomas
authors.initials: T
authors.lastname: Rath
authors.forename: Helmut
authors.initials: H
authors.lastname: Dietrich
authors.forename: Frank
authors.initials: F
authors.lastname: Will
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Stockis
authors.forename: Gerhard
authors.initials: G
authors.lastname: Eisenbrand
authors.forename: Christine
authors.initials: C
authors.lastname: Janzowski
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000872:Anthocyanins / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D001628:Beverages; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry*; D005978:Glutathione / Q000097:blood; D006801:Humans; D006835:Hydrazones / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D016328:NF-kappa B / Q000097:blood; D010084:Oxidation-Reduction / Q000187:drug effects*; D010636:Phenols / Q000378:metabolism*; D010865:Pilot Projects; D059808:Polyphenols; D006435:Renal Dialysis; D018709:Statistics, Nonparametric; D014280:Triglycerides / Q000097:blood
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D006835:Hydrazones; D016328:NF-kappa B; D010636:Phenols; D059808:Polyphenols; D014280:Triglycerides; D008315:Malondialdehyde; D005978:Glutathione