title: In vitro spheroid model of placental vasculogenesis: does it work?
abstract: Placental vascular development begins very early in pregnancy and is characterized by construction of a primitive vascular network in a low-oxygen environment. In vitro three-component assays of this process are scarce. In this study, a complex three-dimensional spheroid model for in vitro studies of placental vasculogenesis with regard to cell-cell interactions between cytotrophoblasts (CTs), villous stromal cells and endothelial precursor cells was established. Microscopic and immunohistochemical analyses of the spheroids showed structural and differentiation patterns resembling the structure and differentiation of early placental chorionic villous tissue (in regard to the expression of multiple markers cytokeratin-7, vimentin, CD34, CD31). The authenticity of this model to in vivo events allowed investigation of placental vascular development and trophoblast invasion under physiological and pathological conditions. Particularly enhanced spheroidal expression of SDF-1alpha and its receptor CXCR4, the major chemokine system in embryonic vasculogenesis, in a low-oxygen environment was detected. In addition, our model confirmed previously described invasive phenotype of trophoblasts through collagen under low- (physiologic), but not high- (pathologic) oxygen concentrations. Therefore, the three-dimensional spheroid model consisting of major placental cell types proved to be an appropriate system to investigate early placental vessel development under both physiological and pathological conditions.
authors.affiliation: Department of Obstetrics and Gynecology, University of Giessen, Giessen, Germany.
authors.forename: Nelli
authors.initials: N
authors.lastname: Baal
authors.forename: Rebecca
authors.initials: R
authors.lastname: Widmer-Teske
authors.forename: Timothy
authors.initials: T
authors.lastname: McKinnon
authors.forename: Klaus T
authors.initials: KT
authors.lastname: Preissner
authors.forename: Marek T
authors.initials: MT
authors.lastname: Zygmunt
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism; D002824:Chorionic Villi / Q000254:growth & development / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010100:Oxygen / Q000008:administration & dosage / Q000378:metabolism; D010920:Placenta / Q000098:blood supply*; D010929:Placentation / Q000502:physiology; D011247:Pregnancy; D019718:Receptors, CXCR4 / Q000378:metabolism; D018874:Spheroids, Cellular / Q000166:cytology* / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology* / Q000502:physiology; D014471:Umbilical Veins / Q000166:cytology; D055815:Young Adult
chemical_list: D015415:Biomarkers; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D010100:Oxygen