[Rapid detection method of three types of bacterial pathogens in aquatic products established by multiplex PCR].
OBJECTIVE
To develop a rapid multiplex PCR (m-PCR) assay for simultaneously detection of three foodborne pathogens in aquatic products.


METHODS
The invasion protein gene (invA) of Salmonella spp., toxR gene (toxR) of Vibrio parahaemolyticus and invasion-associated protein p60 gene (iap) of Listeria monocytogenes were used as the gene targets.


RESULTS
The multiplex PCR assay could be specific and rapid, and the detection limits were 10 cfu/ml when the artificially contaminated aquatic products were incubated at 37 degrees C for 10 h.


CONCLUSIONS
The multiplex PCR assay developed in this study could provide a cost-effective supplement of conventional microbiological methods for routine monitoring of food.
Yang
Xiaojuan
X
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China. xuyang80612@mail.china.com
Wu
Qingping
Q
Zhang
Jumei
J
Xu
Xiaoke
X
D000818:Animals; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000302:isolation & purification*; D017747:Seafood / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification*