title: Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels.
abstract: Bacteriophage phiC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not need perfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the phiC31 integrase approach to make transgenic Xenopus laevis embryos.
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City, IA, USA.
authors.forename: Bryan G
authors.initials: BG
authors.lastname: Allen
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Weeks
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000201:enzymology*; D015139:Blotting, Southern; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D018014:Gene Transfer Techniques; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016662:Virus Integration; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D029867:Xenopus Proteins; D019426:Integrases