title: Determination of levodopa by capillary electrophoresis with chemiluminescence detection.
abstract: A rapid and simple method using capillary electrophoresis (CE) with chemiluminescence (CL) detection was developed for the determination of levodopa. This method was based on enhance effect of levodopa on the CL reaction between luminol and potassium hexacyanoferrate(III) (K(3)[Fe(CN)(6)]) in alkaline aqueous solution. CL detection employed a lab-built reaction flow cell and a photon counter. The optimized conditions for the CL detection were 1.0 x 10(-5)M luminol added to the CE running buffer and 5.0 x 10(-5)M K(3)[Fe(CN)(6)] in 0.6M NaOH solution introduced postcolumn. Under the optimal conditions, a linear range from 5.0 x 10(-8) to 2.5 x 10(-6)M (r=9991), and a detection limit of 2.0 x 10(-8)M (signal/noise=3) for levodopa were achieved. The precision (R.S.D.) on peak area (at 5.0 x 10(-7)M of levodopa, n=11) was 4.1%. The applicability of the method for the analysis of pharmaceutical and human plasma samples was examined.
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China. zhaoshulin001@163.com
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.forename: Wenling
authors.initials: W
authors.lastname: Bai
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: He