Metallothionein and brain injury after intracerebral hemorrhage.
Metallothioneins (MTs) are metal-binding proteins that can be upregulated in the brain after injury and are associated with neuroprotection. A recent genomics study has shown that brain MT-1 and MT-2 mRNA levels are upregulated following intracerebral hemorrhage (ICH) in rats. Our study examines whether brain MT-1 and MT-2 protein levels are increased after ICH. We also investigated the effect of exogenous MT-1 in perihematomal edema formation in vivo and iron-induced cell death in vitro. We found that MT-1/-2 immunoreactivity in ipsilateral basal ganglia was significantly increased after ICH and exogenous MT-1 attenuated perihematomal edema formation. In addition, MT-1 also reduced cell death induced by iron in cultured astrocytes. These results suggest a role for MT in ICH-induced brain injury, and MT could be a therapeutic target for ICH.
Yamashita
S
S
Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI. 48109-2200, USA.
Okauchi
M
M
Hua
Y
Y
Liu
W
W
Keep
R F
RF
Xi
G
G
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001479:Basal Ganglia / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000209:etiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex; D002543:Cerebral Hemorrhage / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
D008668:Metallothionein; D007770:L-Lactate Dehydrogenase