Use of immunohistochemical and PCR methods in diagnosis of infectious haematopoietic necrosis disease in some Rainbow trout hatcheries in Iran.
The aim of this research was investigation on infection in reproducing and breeding centers of Rainbow trout in Iran by infectious Necrosis haematpoietic virus. We collected 100 samples from 100 reproducing and breeding centers of Rainbow trout of the Iran that showed clinical sings or were healthy in appearance in larva stage and finger link. Samples were tested by immunohistochemistry and Nested-PCR methods. By immunohistochemistry methods 35 samples and by PCR 43 samples were positive. There were 38 samples with clinical symptoms of Infectious Haematpoietic Necrosis. The results of this study show the dispersion of Infectious hematopoietic necrosis virus in some reproducing and breeding centers of Rainbow trout in the Iran. Also the result of two methods had acceptable overlapping.
Asl
Adel Haghighi Khiabanian
AH
Department of Pathology, Veterinary Sciences School, Islamic Azad University of Iran, Science and Research Campus, Tehran, Islamic Republic of Iran.
Soltani
Mehdi
M
Kazemi
Bahram
B
Haghdoust
Iraj Sohrabi
IS
Sharifpour
Isa
I
D000818:Animals; D000911:Antibodies, Monoclonal; D017931:DNA Primers / Q000235:genetics; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology*; D005399:Fishes; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D029142:Infectious hematopoietic necrosis virus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D017931:DNA Primers