NOD1 expression in the eye and functional contribution to IL-1beta-dependent ocular inflammation in mice.
OBJECTIVE
NOD1 plays an important role in host defense and recognizes the minimal component of bacterial cell walls, meso-diaminopimelic acid (iE-DAP). Polymorphisms in NOD1 are associated with autoinflammatory diseases characterized by uveitis such as Crohn's disease and sarcoidosis. NOD1 is homologous to NOD2, which is responsible for an autosomal dominant form of uveitis. Nonetheless, the role of NOD1 in intraocular inflammation has not been explored. The induction of uveitis by iE-DAP in mice and the potential contribution of interleukin (IL)-1beta were investigated.


METHODS
BALB/c mice or mice deficient in caspase-1 or IL-1R1 and their congenic controls were injected intravitreally with iE-DAP or saline. The time course, dose response, and contribution of IL-1beta to ocular inflammation were quantified by intravital video microscopy, histology, and immunohistochemistry. NOD1 and IL-1beta were measured in eye tissue by immunoblotting and ELISA.


RESULTS
NOD1 protein is expressed in the eye and promotes ocular inflammation in a dose- and time-dependent fashion. The authors previously defined the role of IL-1beta in NOD2 uveitis and tested whether NOD1 and NOD2 used similar mechanisms. Treatment with iE-DAP significantly increased IL-1beta, which was caspase-1 dependent. However, in contrast to NOD2, caspase-1 and IL-1R1 were essential mediators of iE-DAP-induced uveitis, suggesting that NOD1 and NOD2 induce ocular inflammation by distinct mechanisms involving IL-1beta.


CONCLUSIONS
These findings demonstrate that NOD1 is expressed within the eye and that its activation results in uveitis in an IL-1beta-dependent mechanism. Characterizing the differences between NOD1 and NOD2 responses may provide insight into the pathogenesis of uveitis.
Rosenzweig
Holly L
HL
Casey Eye Institute, Oregon Health & Science University, Portland, Oregon 97239, USA. rosenzwh@ohsu.edu
Galster
Kellen T
KT
Planck
Stephen R
SR
Rosenbaum
James T
JT
D000818:Animals; D020170:Caspase 1 / Q000502:physiology; D003960:Diaminopimelic Acid / Q000633:toxicity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005123:Eye / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007267:Injections; D053583:Interleukin-1beta / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D053474:Nod1 Signaling Adaptor Protein / Q000378:metabolism*; D013997:Time Factors; D014605:Uveitis / Q000378:metabolism*; D014822:Vitreous Body
D053583:Interleukin-1beta; D053474:Nod1 Signaling Adaptor Protein; C507873:Nod1 protein, mouse; D003960:Diaminopimelic Acid; D020170:Caspase 1