[Immunol reconstitution after autologous purified CD34+ cells transplantation in patients with systemic lupus erythematosus].
OBJECTIVE
To investigate the variation of immune index in patients with systemic lupus erythematosus (SLE) treated with autologous purified CD34+ cells transplantation and to clarify the relationship with pathogenesis and prognosis.


METHODS
Flow cytometry (FCM) and enzyme linked immunosorbent assay (ELISA) were used to test lymphocyte subsets, C3, C4, CH50, autoantibodies and immunoglobulin for 18 cases of SLE before and after transplantation.


RESULTS
The results showed that the ratio of all the T cell subsets reduced obviously in early post graft and recovered gradually in 1 to 3 months after transplantation except CD45RO(+)CD4(+) cells. The levels of serum C3, C4, CH50 increased significantly after transplantation. No case relapsed within one year after transplantation, but 2 patients relapsed one year after transplantation. The levels of the indexes in the patients with relapse were significantly lower than those in the patients with persistent remission, including C(4) in the entire course, CH(50) in the 3rd and 12th month after transplantation and CD(45)RA(+)CD(8)+ cells in the 6th month after transplantation. However, the ratio of CD45RO(+)CD(4)+ cells in the first month after transplantation in the patients with relapse was higher than that in the patients with persistent remission.


CONCLUSIONS
Autologous purified CD(34)+ cells transplantation is effective for treating SLE. Survey of immune indexes before and after transplantation is important to investigate the pathogenesis of SLE. Moreover, these immune indexes can be used to predict therapeutic efficacy of SLE.
Wei
Yong-Qiang
YQ
Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Liu
Qi-Fa
QF
Sun
Jing
J
Xu
Dan
D
Yi
Zheng-Shan
ZS
Feng
Ru
R
Meng
Fan-Yi
FY
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D002648:Child; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000601:surgery*; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D016176:T-Lymphocyte Subsets / Q000276:immunology; D019172:Transplantation Conditioning; D014182:Transplantation, Autologous
D018952:Antigens, CD34