Survival of stress exposed Campylobacter jejuni in the murine macrophage J774 cell line.
Although campylobacters are relatively fragile and sensitive to environmental stresses, Campylobacter jejuni has evolved mechanisms for survival in diverse environments, both inside and outside the host. Their survival properties and pathogenic potential were assessed after subjecting food and clinical C. jejuni isolates to different stress conditions. After exposure to starvation (5 h and 15 h of nutrient depletion), a temperature shock (3 min at 55 degrees C) or oxidative stress (5 h and 15 h of atmospheric oxygen) we studied the culturability, viability and capability of adhesion, internalization and survival within the in vitro cell culture model using J774 murine macrophages. Starvation severely impaired C. jejuni culturability, particularly after 15 h of nutrient depletion. The number of viable cells decreased by 30-40%. Starved bacterial cells also showed a lower capability of adhesion, internalization and survival within macrophages. Despite the reduced culturability and viability of the heat treated cells, C. jejuni efficiently adhered to, and entered murine macrophages. However, the number of heat treated cells started to decrease more quickly than non-stressed cells. Within 24 h post infection all the cells were killed. The bacterial mechanisms involved in inactivating toxic oxygen products may enhance bacterial persistence through increased binding, entry and survival of both oxidatively stressed C. jejuni isolates inside the macrophages. Oxygen exposure increased the internalization and intracellular survival, although the cells cannot remain viable for extended periods within murine macrophages. However, any prolongation of survival in macrophages may increase the probability of transmission of bacteria in the host organism and have further implications in the pathogenesis of campylobacteriosis. This indicates that environmental stress conditions may be involved.
Sikić Pogacar
Maja
M
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Rubesa Mihaljević
Roberta
R
Klancnik
Anja
A
Brumini
Gordana
G
Abram
Maja
M
Smole Mozina
Sonja
S
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D016123:Campylobacter jejuni / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*; D002460:Cell Line; D002645:Chickens; D003257:Consumer Product Safety; D005516:Food Microbiology; D006358:Hot Temperature; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008460:Meat / Q000382:microbiology*; D051379:Mice; D050296:Microbial Viability; D010100:Oxygen / Q000494:pharmacology; D014774:Virulence
D010100:Oxygen