Chemopreventive and antilipidperoxidative potential of Clerodendron inerme (L) Gaertn in 7,12-dimethylbenz(a)anthracene induced skin carcinogenesis in Swiss albino mice.
The present study has investigated the chemopreventive and antilipidperoxidative effects of the ethanolic extract of Clerodendron inerme leaves (CiELet) in DMBA induced skin carcinogenesis in Swiss albino mice. The skin squamous cell carcinoma was induced in the shaved back of mice, by painting with DMBA (25 microg 0.1 mL(-1) acetone) twice weekly for 8 weeks. We have observed 100% tumor formation in the fifteenth week of experimental period. Elevated lipid peroxidation and decline in enzymatic and non-enzymatic antioxidants status was observed in tumor bearing mice. Oral administration of CiELet (300 mg kg(-1) bw) for 25 weeks significantly prevented the tumor incidence, volume and burden of the tumor. The CiELet also showed potent antilipidperoxidative effect as well as enhanced the antioxidant defense mechanisms in DMBA painted mice. The present study thus demonstrated the chemopreventive and antilipidperoxidative efficacy of CiELet in DMBA induced mouse skin carcinogenesis.
Renju
Gopi Lilly
GL
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
Manoharan
Shanmugam
S
Balakrishnan
Subramanian
S
Senthil
Namasivayam
N
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D018890:Chemoprevention / Q000379:methods*; D032409:Clerodendrum / Q000033:anatomy & histology / Q000737:chemistry*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*
D000975:Antioxidants; D001551:Benz(a)Anthracenes; D010936:Plant Extracts; C046810:7,12-dihydroxymethylbenz(a)anthracene