title: Nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing.
abstract: Strains TRF-1(T) and TC-9 were isolated from transfer spikes of two separate bottles containing 0.9 % NaCl (physiological saline) solution used repeatedly to wash wounds in hospital wards, months apart. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains TRF-1(T) and TC-9 formed a distinct branch within the family Bacillaceae most closely related to the members of the genus Jeotgalicoccus. The two strains, with identical 16S rRNA gene sequences, showed sequence similarities of 89.8-93.9 % with species of Jeotgalicoccus, 91.1-91.8 % with species of Salinicoccus and 89.1-89.7 % with species of Staphylococcus. Strains TRF-1(T) and TC-9 are Gram-positive, non-spore-forming and non-motile cocci, with an optimum growth temperature of about 37 degrees C. Strain TRF-1(T) grew optimally in medium containing 3 % (w/v) NaCl (maximum about 14 % NaCl), while strain TC-9 grew optimally in medium with 1 % (w/v) NaCl. Both strains produce a brown pigment when grown in the presence of NaCl. The cell-wall peptidoglycan is of the A3alpha type with a cross-linkage containing the peptide l-Lys-Gly(4)-l-Ser-Gly. The major respiratory quinones are menaquinone 7 (MK-7) and menaquinone 8 (MK-8), the major fatty acids are straight-chain C(14 : 0) and C(16 : 0) (more than 85 % of the total) and the major polar lipid is an unknown aminophospholipid. The DNA G+C content is 33.5 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, we are of the opinion that strains TRF-1(T) and TC-9 represent a novel species of a new genus, for which we propose the name Nosocomiicoccus ampullae gen. nov., sp. nov. The type strain of Nosocomiicoccus ampullae is strain TRF-1(T) (=LMG 24060(T) =CIP 109506(T)).
authors.affiliation: Institute of Microbiology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
authors.forename: Marta
authors.initials: M
authors.lastname: Alves
authors.forename: Célia
authors.initials: C
authors.lastname: Nogueira
authors.forename: Augusto
authors.initials: A
authors.lastname: de Magalhães-Sant'ana
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Chung
authors.forename: Paula V
authors.initials: PV
authors.lastname: Morais
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D013045:Species Specificity; D040141:Staphylococcaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D014947:Wounds and Injuries / Q000628:therapy
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride