title: Liquid chromatography/tandem mass spectrometric method for the simultaneous determination of tobacco-specific nitrosamine NNK and its five metabolites.
abstract: A sensitive and robust high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method to analyze 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its five metabolites in one passage was developed and validated. The method achieved excellent reproducibility and accuracy. Linearity was observed for all six compounds (R(2)=0.999) with detection limits (S/N> or =3) ranging from 0.2 to 2.4 pg on column and 0.01-0.12 ng ml(-1) in samples injected. Average intra-day and inter-day variations (% R.S.D.) were 1.2 and 3.5%, respectively. A sample preparation method involving C8 and C18 solid phase extraction provided satisfactory recovery of the analytes in mouse urine. Each NNK metabolite was identified by its chromatographic retention time and specific fragmentation pattern. Since the carcinogenicity of NNK is related to its metabolism, the method described in this report should facilitate toxicological investigations into the carcinogenesis due to NNK exposure in the environment.
authors.affiliation: Division of Environmental Health and Occupational Medicine, National Health Research Institutes, No 35 Keyan Road, Zhunan, Miaoli, Taiwan.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Lee
authors.forename: Chiying
authors.initials: C
authors.lastname: Wang
authors.forename: Susana
authors.initials: S
authors.lastname: Lin
authors.forename: Dennis Paul Hsientang
authors.initials: DP
authors.lastname: Hsieh