Mass spectrometry for pectin structure analysis.
Pectin are extremely complex biopolymers made up of different structural domains. Enzymatic degradation followed by purification and structural analysis of the degradation products proved to be efficient tools for the understanding of pectin fine structure, including covalent interactions between pectic structural domains or with other cell wall polysaccharides. Due to its high sensitivity, high throughput and capacity to analyze mixtures, mass spectrometry has gained more and more importance as a tool for oligosaccharides structural characterization in the past 10 years. This review will focus on the combined use of mass spectrometry and enzymatic digestion for pectins structural characterization.
Ralet
Marie-Christine
MC
UR Biopolymères Interactions Assemblages, INRA, Nantes, France. ralet@nantes.inra.fr
Lerouge
Patrice
P
Quéméner
Bernard
B
D000107:Acetylation; D013058:Mass Spectrometry; D008745:Methylation; D010368:Pectins / Q000737:chemistry* / Q000648:ultrastructure*; D021241:Spectrometry, Mass, Electrospray Ionization; D014284:Triiodothyronine / Q000031:analogs & derivatives
D014284:Triiodothyronine; D010368:Pectins; C043821:carbamylated triiodothyronine