title: Regulation of dendritic cell migration by CD74, the MHC class II-associated invariant chain.
abstract: Dendritic cells (DCs) sample peripheral tissues of the body in search of antigens to present to T cells. This requires two processes, antigen processing and cell motility, originally thought to occur independently. We found that the major histocompatibility complex II-associated invariant chain (Ii or CD74), a known regulator of antigen processing, negatively regulates DC motility in vivo. By using microfabricated channels to mimic the confined environment of peripheral tissues, we found that wild-type DCs alternate between high and low motility, whereas Ii-deficient cells moved in a faster and more uniform manner. The regulation of cell motility by Ii depended on the actin-based motor protein myosin II. Coupling antigen processing and cell motility may enable DCs to more efficiently detect and process antigens within a defined space.
authors.affiliation: INSERM U653, Institut Curie, 12 rue Lhomond, 75005, Paris, France.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Faure-André
authors.forename: Pablo
authors.initials: P
authors.lastname: Vargas
authors.forename: Maria-Isabel
authors.initials: MI
authors.lastname: Yuseff
authors.forename: Mélina
authors.initials: M
authors.lastname: Heuzé
authors.forename: Jheimmy
authors.initials: J
authors.lastname: Diaz
authors.forename: Danielle
authors.initials: D
authors.lastname: Lankar
authors.forename: Veronica
authors.initials: V
authors.lastname: Steri
authors.forename: Jeremy
authors.initials: J
authors.lastname: Manry
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Hugues
authors.forename: Fulvia
authors.initials: F
authors.lastname: Vascotto
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boulanger
authors.forename: Graça
authors.initials: G
authors.lastname: Raposo
authors.forename: Maria-Rosa
authors.initials: MR
authors.lastname: Bono
authors.forename: Mario
authors.initials: M
authors.lastname: Rosemblatt
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Lennon-Duménil
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology; D004705:Endocytosis; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008247:Lysosomes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D024462:Myosin Type II / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; C045084:invariant chain; D002403:Cathepsins; C028217:cathepsin S; D024462:Myosin Type II