title: The epitope characterisation and the osteogenic differentiation potential of human fat pad-derived stem cells is maintained with ageing in later life.
abstract: Some clinical settings are deficient in osteogenic progenitors, e.g. atrophic nonunited fractures, large bone defects, and regions of scarring and osteonecrosis. These benefit from the additional use of bone marrow-derived mesenchymal stem cells, but these cells exhibit an age-related decline in lifespan, proliferation and osteogenic potential. Therapeutic approaches for the repair of bone could be optimised by the identification of a stem cell source that does not show age-related changes. Fat pad-derived stem cells are capable of osteogenesis, but a detailed study of the effect of ageing on their epitope profile and osteogenic potential has so far not been performed. Fat pad-derived cells were isolated from 2 groups of 5 patients with a mean age of 57 years (S.D. 3 years) and 86 years (S.D. 3 years). The proliferation, epitope profile and osteogenic differentiation potential of cells from the 2 groups were compared. Cells isolated from the fat pad of both groups showed similar proliferation rates and exhibited a cell surface epitope profile similar but not identical to that of bone marrow-derived stem cells. The cells from both groups cultured in osteogenic medium exhibited osteogenesis as shown by a significant upregulation of alkaline phosphatase and osteocalcin genes, and significantly greater alkaline phosphatase enzyme activity compared to cells cultured in the control medium. The cells cultured in the osteogenic medium also showed greater calcium phosphate deposition on alizarin red staining. There was no significant difference between the osteogenic potential of the two age groups for any of the parameters studied. The fat pad is a consistent and homogenous source of stem cells that exhibits osteogenic differentiation potential with no evidence of any decline with ageing in later life. This has many potential therapeutic tissue engineering applications for the repair of bone defects in an increasingly ageing population.
authors.affiliation: United Kingdom Centre for Tissue Engineering and Wellcome Trust Centre for Cell Matrix Research, University of Manchester, Oxford Road, Manchester M13 9PT, UK. wasimkhan@doctors.org.uk
authors.forename: W S
authors.initials: WS
authors.lastname: Khan
authors.forename: A B
authors.initials: AB
authors.lastname: Adesida
authors.forename: S R
authors.initials: SR
authors.lastname: Tew
authors.forename: J G
authors.initials: JG
authors.lastname: Andrew
authors.forename: T E
authors.initials: TE
authors.lastname: Hardingham
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000502:physiology*; D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000502:physiology*; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis / Q000502:physiology*
chemical_list: D000469:Alkaline Phosphatase