Accumulation of the bioactive peptides, novokinin, LPYPR and rubiscolin, in seeds of genetically modified soybean.
Novokinin (RPLKPW), LPYPR, and rubiscolin (YPLDLF) are bioactive peptides with respective hypotensive, hypocholesterolemic, and memory-enhancing activities. We generated transgenic soybean lines that expressed modified forms of the alpha' subunit of seed storage protein beta-conglycinin containing tandem repeats of these bioactive peptides. The modified alpha' subunits constituted up to 0.2% of extracted proteins from the transgenic seeds.
Nishizawa
Keito
K
National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido, Japan.
Kita
Akiko
A
Doi
Chieko
C
Yamada
Yuko
Y
Ohinata
Kousaku
K
Yoshikawa
Masaaki
M
Ishimoto
Masao
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D030841:Food, Genetically Modified; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism*
C511871:Arg-Pro-Leu-Lys-Pro-Trp; D009842:Oligopeptides