title: Evaluation of neurotoxicity of repeated dermal application of chlorpyrifos on hippocampus of adult mice.
abstract: Dermal absorption of chlorpyrifos, an organophosphate insecticide is important because of its use in agriculture and control of household pests. The objectives of this study are to investigate firstly, the biochemical changes in the blood and secondly, histomorphometric changes in the hippocampus of adult mice following dermal application of chlorpyrifos in sub-toxic doses. Male Swiss albino mice (60 days) were segregated into one control and two treated groups (n=10). Chlorpyrifos, diluted with xylene, was applied in doses of 1/2 of LD(50) (E1) and 1/5 of LD(50) (E2) over the tail of mice of the two treated groups, 6 hours daily for 3 weeks. AChE levels in the serum and brain were estimated using a spectrophotometric method (Amplex Red reagent). Coronal serial sections were stained with 0.2 % thionin in acetate buffer and pyramidal neurons of Cornu Ammonis of hippocampus were counted at 400x magnification using Image Pro Express software. At the end of 3 weeks, body weights were reduced significantly in E1 group. Serum AChE concentrations were reduced by 97 % in E1 and 74 % in E2 groups compared to controls. The neurons of CA 3 and CA 1 in the hippocampus showed evidences of morphological damage in both treated groups. Furthermore, the neuronal count was significantly reduced in CA 3 layer of hippocampus in E1 group.
authors.affiliation: Human Biology Department, International Medical University, No. 126 Jalan 19/155B, Bukit Jalil, Kuala Lumpur, Malaysia. nileshkumar_mitra@imu.edu.my
authors.forename: Nilesh K
authors.initials: NK
authors.lastname: Mitra
authors.forename: How Hee
authors.initials: HH
authors.lastname: Siong
authors.forename: Vishna D
authors.initials: VD
authors.lastname: Nadarajah
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000279:Administration, Cutaneous; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007306:Insecticides / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D011897:Random Allocation
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D000110:Acetylcholinesterase; D004390:Chlorpyrifos