Nmur1-/- mice are not protected from cutaneous inflammation.
Neuromedin U (Nmu) is a neuropeptide expressed primarily in the gastrointestinal tract and central nervous system. Previous reports have identified two G protein-coupled receptors (designated Nmur1 and Nmur2) that bind Nmu. Recent reports suggest that Nmu mediates immune responses involving mast cells, and Nmur1 has been proposed to mediate these responses. In this study, we generated mice with an Nmur1 deletion and then profiled the responses of these mice in a cutaneous inflammation model utilizing complete Freund's adjuvant (CFA). We report here that mice lacking Nmur1 had normal inflammation responses with moderate changes in serum cytokines compared to Nmur1(+/+) littermates. Although differences in IL-6 were observed in mice lacking Nmu peptide, these mice exhibited a normal response to CFA. Our data argues against a major role for Nmur1 in mediating the reported inflammatory functions of NmU.
Abbondanzo
Susan J
SJ
Department of Discovery Technology, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. susan.abbondanzo@spcorp.com
Manfra
Denise J
DJ
Chen
Shu-Cheng
SC
Pinzon-Ortiz
Maria
M
Sun
Yongliang
Y
Phillips
Jonathan E
JE
Laverty
Maureen
M
Vassileva
Galya
G
Hu
Weiwen
W
Yang
Shijun
S
Gustafson
Eric L
EL
Fine
Jay S
JS
Hedrick
Joseph A
JA
D000818:Animals; D016207:Cytokines / Q000097:blood; D003872:Dermatitis / Q000235:genetics / Q000276:immunology*; D005620:Freund's Adjuvant / Q000276:immunology / Q000494:pharmacology; D017353:Gene Deletion; D051379:Mice; D018345:Mice, Knockout; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000502:physiology*
D016207:Cytokines; D017981:Receptors, Neurotransmitter; C082444:neuromedin U receptor; D005620:Freund's Adjuvant