Synthesis of acyl carrier protein using a new heterocyclic carboxyl activating group, 3-mercapto-5,6-diphenyl-1,2,4-triazine through SPPS strategy.
A new, effective and easily accessible carboxyl activating group , 3-mercapto-5, 6 - diphenyl-1, 2, 4 - triazine was developed and the effectiveness was investigated using acyl derivatives, chemically by the preparation of respective amides, in addition to monitoring spectrophotometrically exploiting the UV- visible spectra. The carboxyl activating nature of the above mercapto heterocycle was further confirmed by solid phase peptide synthesis. Here CLPSER resin as polymeric support was applied. We synthesized Acyl Carrier Protein and smaller peptides by replacing this thiol for other conventional carboxyl activating reagents. These peptides synthesized were purified by HPLC and characterized by molecular weight method.
Rajan
M P
MP
Dept. of Chemistry, University of Calicut, 673635, Kerala, India. rajan.mp@rediffmail.com
Purushothaman
E
E
D000179:Acrylates / Q000737:chemistry; D000213:Acyl Carrier Protein / Q000138:chemical synthesis*; D000596:Amino Acids / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000737:chemistry*; D008745:Methylation; D010446:Peptide Fragments / Q000138:chemical synthesis; D011092:Polyethylene Glycols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013997:Time Factors; D014227:Triazines / Q000737:chemistry*
C534542:3-mercapto-5,6-diphenyl-1,2,4-triazine; D000179:Acrylates; D000213:Acyl Carrier Protein; D000596:Amino Acids; D003432:Cross-Linking Reagents; D006571:Heterocyclic Compounds; D010446:Peptide Fragments; D011137:Polystyrenes; D013438:Sulfhydryl Compounds; D014227:Triazines; D011092:Polyethylene Glycols