Cloning and expression of a human tissue plasminogen activator variant: K2S in Escherichia coli.
The DNA sequence of Kringle-2 and serine protease domains of the human tissue plasminogen activator (reteplase, K2S) was PCR amplified. This product was then cloned into the expression vector pET15b plasmid. The presence of the insert was confirmed by restriction digestion, PCR and determination of the nucleotide sequence. By using isopropyl beta-D thiogalactopyranoside (IPTG), reteplase was induced in E. coli BL21 cells and analyzed using polyacrylamide gel electrophoresis (PAGE).
Dormiani
Kianoush
K
Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Khazaie
Yahya
Y
Sadeghi
Hamid Mir Mohammad
HM
Rabbani
Mohammad
M
Moazen
Fatemeh
F
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D007544:Isopropyl Thiogalactoside; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D010959:Tissue Plasminogen Activator / Q000235:genetics* / Q000378:metabolism*
D017931:DNA Primers; D007544:Isopropyl Thiogalactoside; D010959:Tissue Plasminogen Activator