Effect of vitamin levels on biomarkers of exposure and oxidative damage-the EXPAH study.
DNA adducts are markers of carcinogen exposure and of their biological effect; they have been shown to be related to mutagenesis, and therefore they could be a predictive biomarker of human cancer. The objective of this study was to assess if there is a relationship between vitamins A, C, and E, which are known to play a significant role as free radical scavengers and antioxidant agents, and biomarkers of genotoxicity and oxidative stress. Three hundred and fifty-six subjects from Czech Republic, Slovak Republic and Bulgaria, who completed a questionnaire on dietary information and had a measurement of plasma A, C, E vitamins, DNA adduct levels (benzo[a]pyrene (B[a]P) and bulky (DNA-Tot) DNA adducts) and oxidative damage (cyclic pyrimidopurinone N-1,N2 malondialdehyde-2 deoxyguanosine (M1dG) and 8-oxo-7,8-dihydro-2_deoxyguanosine (8-oxodG)) were analyzed. A significant inverse correlation was observed between plasma vitamin levels and both benzo[a]pyrene (B[a]P) and bulky DNA adducts. Vitamin A was also significantly inversely correlated with M1dG, a marker of oxidative damage. The associations were stronger in non-smokers than in smokers. Dietary intake of certain antioxidants such as vitamins is associated with reduced levels of markers of DNA damage (B[a]P and DNA-Tot) and oxidation (M1dG and 8-oxodG) measured in peripheral white blood cells. This could contribute to the protective role of such a dietary pattern on cancer risk. The protective effect of dietary vitamins is less evident in smokers.
Sram
R J
RJ
Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine of Academy AS CR and Health Institute of Central Bohemia, Prague, Czech Republic.
Farmer
P
P
Singh
R
R
Garte
S
S
Kalina
I
I
Popov
T A
TA
Binkova
B
B
Ragin
C
C
Taioli
E
E
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis*; D003430:Cross-Sectional Studies; D018736:DNA Adducts / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D011795:Surveys and Questionnaires; D014815:Vitamins / Q000008:administration & dosage* / Q000494:pharmacology*
D015415:Biomarkers; D018736:DNA Adducts; D014815:Vitamins