Characterization and expression of NADPH oxidase in LPS-, poly(I:C)- and zymosan-stimulated trout (Oncorhynchus mykiss W.) macrophages.
In vertebrates, the generation of superoxide reactive oxygen species (ROS) via activation of the Nox/Duox family of NADPH oxidases is a prototypical feature of the pathogen-induced defensive responses of activated professional phagocytes. To understand the role of the rainbow trout (Oncorhynchus mykiss) Phox oxidase from a phylogenetic and functional perspective we describe the cloning, sequencing and expression analysis of multiple NADPH components in cultured macrophages. Phylogenetic analyses support the notion of the emergence of Phox-related components before the diversification of basal euteleosts and add to the limited collection of teleost NADPH oxidases. Expression studies using lipopolysaccharide, polyinosine-polycytidylic acid and zymosan to mimic the onset of inflammatory responses in trout macrophages suggest differences in regulation of the NADPH complex throughout the maturation/differentiation period of culture and between different treatments.
Boltaña
Sebastian
S
Unitat de Fisiologia Animal, Departament de Biologia Cellular, Fisiologia i d'Immunologia, Facultat de Biociencies, Universitat Autònoma de Barcelona, Barcelona, Spain.
Doñate
Carmen
C
Goetz
Frederick W
FW
MacKenzie
Simon
S
Balasch
Joan C
JC
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000145:classification / Q000276:immunology*; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D016415:Sequence Alignment; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D015054:Zymosan; D019255:NADPH Oxidases; D011070:Poly I-C