Constitutive expression of a rice GTPase-activating protein induces defense responses.
G-proteins (guanine nucleotide-binding proteins that usually exhibit GTPase activities) and related signal transduction processes play important roles in mediating plant defense responses; here, a rice (Oryza sativa) cDNA clone, OsGAP1, encoding a GTPase-activating protein (GAP) that also contains a protein kinase C conserved region 2 (C2) domain is reported. An interacting G-protein partner for the OsGAP1 protein was identified by yeast two-hybrid library screening and confirmed by co-immunoprecipitation; the GTPase-activation activity of OsGAP1 on this interacting G-protein was demonstrated using in vitro assays. OsGAP1 was induced by wounding in rice and the presence of the R locus Xa14 enhances such induction. Gain-of-function tests in transgenic rice and Arabidopsis thaliana showed that constitutive expression of OsGAP1 led to increased resistance to bacterial pathogens in both monocots and dicots.
Cheung
Ming-Yan
MY
Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
Zeng
Nai-Yan
NY
Department of Biology and.
Tong
Suk-Wah
SW
Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
Li
Wing-Yen Francisca
WF
Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
Xue
Yan
Y
State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
Zhao
Kai-Jun
KJ
Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
Wang
Chunlian
C
Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
Zhang
Qi
Q
Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
Fu
Yaping
Y
China National Rice Research Institute, Hangzhou, China.
Sun
Zongxiu
Z
China National Rice Research Institute, Hangzhou, China.
Sun
Samuel Sai-Ming
SS
Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
Lam
Hon-Ming
HM
Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism; D003001:Cloning, Molecular; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism*; D010935:Plant Diseases; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D020690:GTPase-Activating Proteins; D010940:Plant Proteins