Challenges in applying photoemission electron microscopy to biological systems.
Photoemission electron microscopy (PEEM) is a unique surface-sensitive instrument capable of providing real-time images with high spatial resolution. While similar to the more common electron microscopies, scanning electron microscopy and transmission electron microscopy, the imaging technology relies on the photogeneration of electrons emitted from a sample through light excitation. This imaging technique has found prominence in surface and materials sciences, being well suited for imaging flat surfaces, and changes that occur to that surface as various parameters are changed (e.g. temperature, exposure to reactive gases). Biologically focused PEEM received significant attention in the 1970s, but was not aggressively advanced since that pioneering work. PEEM is capable of providing important insights into biological systems that extend beyond simple imaging. In this article, we identify and establish important issues that affect the acquisition and analysis of biological samples with PEEM. We will briefly review the biological impact and importance of PEEM with respect to our work. The article also concludes with a discussion of some of the current challenges that must be addressed to enable PEEM to achieve its maximum potential with biological samples.
Peles
Dana N
DN
Department of Chemistry, Duke University, Durham, NC, USA.
Simon
John D
JD
D001686:Biological Phenomena; D006801:Humans; D008854:Microscopy, Electron / Q000295:instrumentation* / Q000379:methods*; D010777:Photochemistry; D010860:Pigments, Biological
D010860:Pigments, Biological