title: Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes.
abstract: Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses or other coronaviruses were used or when mammalian cells were stimulated with IBV. Inactivation of IBV reduced ChIFN-gamma production, but ChIFN-gamma remained elevated compared to unstimulated cells. An increase in ChIFN-gamma mRNA was detected in splenocytes from IBV-infected and uninfected chickens as early as 1 h after stimulation with IBV. These results indicate that IBV acts as a polyclonal stimulus, inducing a rapid production of IFN-gamma even without previous exposure to the virus.
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, The Netherlands.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Ariaans
authors.forename: Peter M
authors.initials: PM
authors.lastname: van de Haar
authors.forename: Evert J
authors.initials: EJ
authors.lastname: Hensen
authors.forename: Lonneke
authors.initials: L
authors.lastname: Vervelde
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D001351:Infectious bronchitis virus / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D007958:Leukocyte Count; D007962:Leukocytes / Q000821:virology*; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D007371:Interferon-gamma