title: Rapid cell mapping using nanoparticles and SERRS.
abstract: Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
authors.affiliation: Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UKG1 1XL.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Stokes
authors.forename: Fiona
authors.initials: F
authors.lastname: McKenzie
authors.forename: Emma
authors.initials: E
authors.lastname: McFarlane
authors.forename: Alastair
authors.initials: A
authors.lastname: Ricketts
authors.forename: Laurence
authors.initials: L
authors.lastname: Tetley
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.forename: James
authors.initials: J
authors.lastname: Alexander
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
mesh_terms: D004396:Coloring Agents; D006046:Gold; D008264:Macrophages / Q000648:ultrastructure*; D053758:Nanoparticles; D012680:Sensitivity and Specificity; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D004396:Coloring Agents; D012834:Silver; D006046:Gold