Interaction of the human somatostatin receptor 3 with the multiple PDZ domain protein MUPP1 enables somatostatin to control permeability of epithelial tight junctions.
The presence of heterotrimeric G-proteins at epithelial tight junctions suggests that these cellular junctions are regulated by so far unknown G-protein coupled receptors. We identify here an interaction between the human somatostatin receptor 3 (hSSTR3) and the multiple PDZ protein MUPP1. MUPP1 is a tight junction scaffold protein in epithelial cells, and as a result of the interaction with MUPP1 the hSSTR3 is targeted to tight junctions. Interaction with MUPP1 enables the receptor to regulate transepithelial permeability in a pertussis toxin sensitive manner, suggesting that hSSTR3 can activate G-proteins locally at tight junctions.
Liew
Chong Wee
CW
Institut für Zellbiochemie und klinische Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany.
Vockel
Matthias
M
Glassmeier
Günter
G
Brandner
Johanna M
JM
Fernandez-Ballester
Gregorio J
GJ
Schwarz
Jürgen R
JR
Schulz
Stefan
S
Buck
Friedrich
F
Serrano
Luis
L
Richter
Dietmar
D
Kreienkamp
Hans-Jürgen
HJ
D002352:Carrier Proteins / Q000378:metabolism*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins; D054731:PDZ Domains; D010539:Permeability / Q000187:drug effects; D037342:Pertussis Toxin / Q000494:pharmacology; D017481:Receptors, Somatostatin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; C111442:MPDZ protein, human; D008565:Membrane Proteins; D017481:Receptors, Somatostatin; C103631:somatostatin receptor 3; D013004:Somatostatin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins