title: FCA does not bind abscisic acid.
abstract: The RNA-binding protein FCA promotes flowering in Arabidopsis. Razem et al. reported that FCA is also a receptor for the phytohormone abscisic acid (ABA). However, we find that FCA does not bind ABA, suggesting that the quality of the proteins assayed and the sensitivity of the ABA-binding assay have led Razem et al. to erroneous conclusions. Because similar assays have been used to characterize other ABA receptors, our results indicate that the ABA-binding properties of these proteins should be carefully re-evaluated and that alternative ABA receptors are likely to be discovered.
authors.affiliation: Biochemistry Department, University of Otago, Dunedin 9054, New Zealand. catherine.day@otago.ac.nz.
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Risk
authors.forename: Richard C
authors.initials: RC
authors.lastname: Macknight
authors.forename: Catherine L
authors.initials: CL
authors.lastname: Day
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C518020:FY protein, Arabidopsis; C106535:Flowering time control protein FCA, Arabidopsis; D016601:RNA-Binding Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D000040:Abscisic Acid