In vitro nuclear assembly using fractionated Xenopus egg extracts.
Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
Cross
Marie
M
Department of Cell Biology, Emory University, USA. mkcross@emory.edu
Powers
Maureen
M
D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D008297:Male; D010063:Ovum / Q000737:chemistry / Q000166:cytology*; D013094:Spermatozoa / Q000737:chemistry; D014981:Xenopus / Q000502:physiology*
D002457:Cell Extracts; D002843:Chromatin