title: Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells.
abstract: OBJECTIVE
  To identify adiponectin and its receptors (AdipoR1 and AdipoR2) in human granulosa cells (GC) and to study the effects of recombinant human adiponectin on P and E(2) secretion from these cells.
  
  
  METHODS
  The effects of recombinant human adiponectin on the secretion of P and E(2) by cultured human GCs were investigated.
  
  
  METHODS
  Academic institutions.
  
  
  METHODS
  Seventeen infertile and healthy women undergoing IVF.
  
  
  METHODS
  Primary human GC cultures stimulated with human recombinant adiponectin (5 microg/mL).
  
  
  METHODS
  Determination of messenger RNA (mRNA) and protein expression of adiponectin and its receptors AdipoR1 and AdipoR2 in fresh human GCs by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot, respectively. Measurement of P and E(2) levels in the conditioned media by RIA and determination of cell proliferation by tritied thymidine incorporation.
  
  
  RESULTS
  Human GCs express adiponectin receptors AdipoR1 and AdipoR2 but not adiponectin. In primary human GCs, adiponectin increases P and E(2) secretion in response to insulin-like growth factor I (IGF-I). This was associated with an increase in the p450 aromatase protein level but not those of p450scc, 3 beta HSD, or StAR. Adiponectin treatment does not affect IGF-1-induced cell proliferation and basal steroidogenesis (no IGF-1 or FSH stimulation). Adiponectin rapidly stimulates MAPK ERK1/2 and p38 phosphorylation in primary human GCs.
  
  
  CONCLUSIONS
  Adiponectin receptors AdipoR1 and AdipoR2, but not adiponectin, are present in human GCs. Adiponectin increases IGF-1-induced P and E(2) secretion in primary human GCs.
authors.affiliation: Unité de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, Nouzilly, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Chabrolle
authors.forename: Lucie
authors.initials: L
authors.lastname: Tosca
authors.forename: Christelle
authors.initials: C
authors.lastname: Ramé
authors.forename: Pierre
authors.initials: P
authors.lastname: Lecomte
authors.forename: Dominique
authors.initials: D
authors.lastname: Royère
authors.forename: Joëlle
authors.initials: J
authors.lastname: Dupont
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000378:metabolism / Q000494:pharmacology; D015571:Follicular Fluid / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism* / Q000503:physiopathology; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology; D054315:Oocyte Retrieval; D011374:Progesterone / Q000378:metabolism*; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: C499918:ADIPOQ protein, human; C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D054419:Receptors, Adiponectin; D011994:Recombinant Proteins; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone