title: Molecular identification and susceptibility testing of Trichosporon isolates from a Brazilian hospital.
abstract: In this study the molecular identification and susceptibility profile of 21 clinical isolates, from a Brazilian hospital, belongs to six different species of Trichosporon were described. Trichosporon asahii was the predominant species and corresponded to 43% of isolates. Eighty three percent of the strains isolated from deep sites were identified as T. asahii, while only 17% belong to a non-T. asahii species (Trichosporon inkin). In general, the MICs were high and independent of the species of Trichosporon as well as the clinical origin of strain. Amphotericin B and fluconazole were less effective against Trichosporon spp. and high MIC values of voriconazole, posaconazole and ravuconazole were observed. Fifty-six percent (5/9) of T. asahii strains were isolated from deep sites, whereas 8% (1/12) of non-T. asahii species were isolated from those sites. A total of 89% of T. asahii isolates exhibited resistance to amphotericin B in vitro.
authors.affiliation: Nucleo de Doenças Infecciosas, Universidade Federal do Espirito Santo, Brazil. mariceliaraujo@yahoo.com
authors.forename: Mariceli
authors.initials: M
authors.lastname: Araujo Ribeiro
authors.forename: Ana
authors.initials: A
authors.lastname: Alastruey-Izquierdo
authors.forename: Alicia
authors.initials: A
authors.lastname: Gomez-Lopez
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Rodriguez-Tudela
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D003881:Dermatomycoses / Q000382:microbiology; D025141:Drug Resistance, Fungal; D054714:Echinocandins / Q000494:pharmacology; D005260:Female; D005437:Flucytosine / Q000494:pharmacology; D016469:Fungemia / Q000382:microbiology; D006785:Hospitals, University / Q000706:statistics & numerical data; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods; D009181:Mycoses / Q000453:epidemiology / Q000382:microbiology*; D014009:Onychomycosis / Q000382:microbiology; D009894:Opportunistic Infections / Q000453:epidemiology / Q000382:microbiology; D009928:Organ Specificity; D014230:Triazoles / Q000494:pharmacology; D014250:Trichosporon / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D014556:Urine / Q000382:microbiology; D014627:Vaginitis / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D014230:Triazoles; D000666:Amphotericin B; D005437:Flucytosine