Amino acids with an intermolecular proton bond as proton storage site in bacteriorhodopsin.
The positions of protons are not available in most high-resolution structural data of biomolecules, thus the identity of proton storage sites in biomolecules that transport proton is generally difficult to determine unambiguously. Using combined quantum mechanical/molecular mechanical computations, we demonstrate that a pair of conserved glutamate residues (Glu 194/204) bonded by a delocalized proton is the proton release group that has been long sought in the proton pump, bacteriorhodopsin. This model is consistent with all available experimental structural and infrared data for both the wild-type bacteriorhodopsin and several mutants. In particular, the continuum infrared band in the 1,800- to 2,000-cm(-1) region is shown to arise due to the partially delocalized nature of the proton between the glutamates in the wild-type bacteriorhodopsin; alternations in the flexibility of the glutamates and electrostatic nature of nearby residues in various mutants modulate the degree of proton delocalization and therefore intensity of the continuum band. The strong hydrogen bond between Glu 194/204 also significantly shifts the carboxylate stretches of these residues well <1,700 cm(-1), which explains why carboxylate spectral shift was not observed experimentally in the typical >1,700-cm(-1) region upon proton release. By contrast, simulations with the proton restrained on the nearby water cluster, as proposed by several recent studies [see, for example, Garezarek K, Gerwert K (2006) Functional waters in intraprotein proton transfer monitored by FTIR difference spectroscopy. Nature 439:109], led to significant structural deviations from available X-ray structures. This study establishes a biological function for strong, low-barrier hydrogen bonds.
Phatak
Prasad
P
Institute of Physical and Theoretical Chemistry, Technische Universität Braunschweig, Hans-Sommer Strasse 10, 38106 Braunschweig, Germany.
Ghosh
Nilanjan
N
Yu
Haibo
H
Cui
Qiang
Q
Elstner
Marcus
M
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000235:genetics; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D018698:Glutamic Acid / Q000737:chemistry*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009154:Mutation; D017433:Protein Structure, Secondary; D011522:Protons; D011789:Quantum Theory; D013055:Spectrophotometry, Infrared; D055672:Static Electricity
D011522:Protons; D018698:Glutamic Acid; D001436:Bacteriorhodopsins