title: Common transcriptional signature of tumor-infiltrating mononuclear inflammatory cells and peripheral blood mononuclear cells in hepatocellular carcinoma patients.
abstract: Hepatocellular carcinoma (HCC) is frequently associated with infiltrating mononuclear inflammatory cells. We performed laser capture microdissection of HCC-infiltrating and noncancerous liver-infiltrating mononuclear inflammatory cells in patients with chronic hepatitis C (CH-C) and examined gene expression profiles. HCC-infiltrating mononuclear inflammatory cells had an expression profile distinct from noncancerous liver-infiltrating mononuclear inflammatory cells; they differed with regard to genes involved in biological processes, such as antigen presentation, ubiquitin-proteasomal proteolysis, and responses to hypoxia and oxidative stress. Immunohistochemical analysis and gene expression databases suggested that the up-regulated genes involved macrophages and Th1 and Th2 CD4 cells. We next examined the gene expression profile of peripheral blood mononuclear cells (PBMC) obtained from CH-C patients with or without HCC. The expression profiles of PBMCs from patients with HCC differed significantly from those of patients without HCC (P < 0.0005). Many of the up-regulated genes in HCC-infiltrating mononuclear inflammatory cells were also differentially expressed by PBMCs of HCC patients. Analysis of the commonly up-regulated or down-regulated genes in HCC-infiltrating mononuclear inflammatory cells and PBMCs of HCC patients showed networks of nucleophosmin, SMAD3, and proliferating cell nuclear antigen that are involved with redox status, the cell cycle, and the proteasome system, along with immunologic genes, suggesting regulation of anticancer immunity. Thus, exploring the gene expression profile of PBMCs may be a surrogate approach for the assessment of local HCC-infiltrating mononuclear inflammatory cells.
authors.affiliation: Department of Gastroenterology, Kanazawa University, School of Medicine, Kanazawa, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sakai
authors.forename: Masao
authors.initials: M
authors.lastname: Honda
authors.forename: Haruo
authors.initials: H
authors.lastname: Fujinaga
authors.forename: Isamu
authors.initials: I
authors.lastname: Tatsumi
authors.forename: Eishiro
authors.initials: E
authors.lastname: Mizukoshi
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Nakamoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006521:Hepatitis, Chronic / Q000097:blood / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008103:Liver Cirrhosis / Q000097:blood / Q000235:genetics; D008113:Liver Neoplasms / Q000097:blood* / Q000235:genetics*; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic; D015854:Up-Regulation