title: Gene regulation in continuous cultures: a unified theory for bacteria and yeasts.
abstract: During batch growth on mixtures of two growth-limiting substrates, microbes consume the substrates either sequentially (diauxie) or simultaneously. The ubiquity of these growth patterns suggests that they may be driven by a universal mechanism common to all microbial species. Recently, we showed that a minimal model accounting only for enzyme induction and dilution, the two processes that occur in all microbes, explains the phenotypes observed in batch cultures of various wild-type and mutant/recombinant cells (Narang and Pilyugin in J. Theor. Biol. 244:326-348, 2007). Here, we examine the extension of the minimal model to continuous cultures. We show that: (1) Several enzymatic trends, attributed entirely to cross-regulatory mechanisms, such as catabolite repression and inducer exclusion, can be quantitatively explained by enzyme dilution. (2) The bifurcation diagram of the minimal model for continuous cultures, which classifies the substrate consumption pattern at any given dilution rate and feed concentrations, provides a precise explanation for the empirically observed correlations between the growth patterns in batch and continuous cultures. (3) Numerical simulations of the model are in excellent agreement with the data. The model captures the variation of the steady state substrate concentrations, cell densities, and enzyme levels during the single- and mixed-substrate growth of bacteria and yeasts at various dilution rates and feed concentrations. This variation is well approximated by simple analytical expressions that furnish deep physical insights. (4) Since the minimal model describes the behavior of the cells in the absence of cross-regulatory mechanisms, it provides a rigorous framework for quantifying the effect of these mechanisms. We illustrate this by analyzing several data sets from the literature.
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville, FL 32611-6005, USA.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Noel
authors.forename: Atul
authors.initials: A
authors.lastname: Narang
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques; D004790:Enzyme Induction / Q000235:genetics; D004794:Enzyme Repression / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008660:Metabolism / Q000235:genetics; D015003:Yeasts / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*