[Effects of paclitaxel on the macrophages derived from bone marrow cells in vitro].
OBJECTIVE
To Study on the effects of paclitaxel on macrophages derived from the mouse bone marrow cells.


METHODS
The bone marrow cells flushed from BALB/c tibia, femur and hipbone were resuspended in RPMI1640 containing M-CSF and seeded in six-well plates. Then they were cultured in RPMI1640 containing M-CSF in the presence or absence of paclitaxel for seven days. The phenotypes and phagocytosis of the macrophages derived from mice bone marrow cells were measured by FCM and their antigen presentation was detected by DTH.


RESULTS
The expression of CD80 and CD14 in paclitaxal-treated macrophages was increased and their function of phagocytosis was significantly enhanced. More reduction appeared in DTH reaction of the macrophages treated with palitaxal compared with that in the macrophages derived from control group.


CONCLUSIONS
Paclitaxel can significantly enhance the phagocytosis of the macrophages derived from bone marrow cells and decrease their immunogenicity, which indicates that paclitaxal may regulate the immunological function of the macrophages derived from bone marrow cells.
Li
Long
L
Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China. long.lil81@yahoo.com.cn
We
Hong
H
Chen
Jing
J
Zhao
Yong
Y
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D018122:B7-1 Antigen / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017239:Paclitaxel / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D018122:B7-1 Antigen; D018950:Lipopolysaccharide Receptors; D016173:Macrophage Colony-Stimulating Factor; D017239:Paclitaxel