Identification of full-length proviral DNA of porcine endogenous retrovirus from Chinese Wuzhishan miniature pigs inbred.
Existence of porcine endogenous retrovirus (PERV) hinders pigs to be used in clinical xenotransplantation to alleviate the shortage of human transplants. Chinese miniature pigs are potential organ donors for xenotransplantation in China. However, so far, an adequate level of information on the molecular characteristics of PERV from Chinese miniature pigs has not been available. We described here the cloning and characterization of full-length proviral DNA of PERV from Chinese Wuzhishan miniature pigs inbred (WZSP). Full-length nucleotide sequences of PERV-WZSP and other PERVs were aligned and phylogenetic tree was constructed from deduced amino-acid sequences of env. The results demonstrated that the full-length proviral DNA of PERV-WZSP belongs to gammaretrovirus and shares high similarity with other PERVs. Sequence analysis also suggested that different patterns of LTR existed in the same porcine germ line and partial PERV-C sequence may recombine with PERV-A sequence in LTR.
Ma
Yuyuan
Y
Laboratory for Viral Safety of National Centre of Biomedical Analysis, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
Lv
Maomin
M
Xu
Shu
S
Wu
Jianmin
J
Tian
Kegong
K
Zhang
Jingang
J
D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000302:isolation & purification*; D010802:Phylogeny; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification*; D012192:Retroviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D013556:Swine, Miniature
D004279:DNA, Viral