Development of a new high performance low pressure chromatographic system using a multisyringe burette coupled to a chromatographic monolithic column.
A novel combination of high performance low pressure chromatography with multisyringe flow injection analysis is presented. This system comprises a multisyringe module, three low pressure solenoid valves, a monolithic Chromolith Flash RP-18e column and a diode array spectrophotometer. UV detection is carried out at 250nm. AutoAnalysis software is used for instrumental control and automated data collection. The results obtained with multisyringe liquid chromatography (MSC) were compared with those obtained with a HPLC system using similar conditions. The chromatographic parameters were calculated from a mixture of anthracene and thiourea using a mobile phase containing acetonitrile-water (60:40) at a flow rate of 2mlmin(-1). The proposed MSC system has been successfully applied to the determination of amoxicillin, ampicillin and cephalexin using a mobile phase of sodium acetate buffer (pH 6.2, 0.1moll(-1))-methanol (90:10) at a flow rate of 2mlmin(-1). The low-cost, flexibility and simplicity of MSC should be highlighted.
Miguel
Hilda M González-San
HM
Department of Quality Assurance and Pharmaceutical Technology, Food and Pharmaceutical Sciences Institute, University of Havana, Cuba.
Alpízar-Lorenzo
Jesús M
JM
Cerdà-Martín
Víctor
V