title: Upregulation of S100 calcium-binding protein A9 is required for induction of smooth muscle cell proliferation by a periodontal pathogen.
abstract: We investigated the effect of a periodontal pathogen, Porphyromonas gingivalis, on human aortic smooth muscle cell (hAOSMC) proliferation as mechanisms of atherosclerosis. Cultured hAOSMCs exposed to the supernatant of plasma incubated with P. gingivalis showed a marked transformation from a contractile to proliferative phenotype, resulting in enhancement of cell growth. DNA microarray analysis revealed a P. gingivalis-dependent upregulation of S100A9 in hAOSMCs. Small interference-RNA for S100A9 dramatically attenuated the effect of P. gingivalis on transformation and proliferation of hAOSMCs. Our data suggested that upregulation of S100A9 mediated by P. gingivalis is an important event in the development of aortic intimal hyperplasia.
authors.affiliation: Department of Oral Frontier Biology, Graduate School of Dentistry, Osaka University, Suita 565-0871, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Inaba
authors.forename: Kazuya
authors.initials: K
authors.lastname: Hokamura
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakano
authors.forename: Ryota
authors.initials: R
authors.lastname: Nomura
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Katayama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakajima
authors.forename: Hideo
authors.initials: H
authors.lastname: Yoshioka
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kamisaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Ooshima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
authors.forename: Ferid
authors.initials: F
authors.lastname: Murad
authors.forename: Koichiro
authors.initials: K
authors.lastname: Wada
authors.forename: Atsuo
authors.initials: A
authors.lastname: Amano
mesh_terms: D001011:Aorta / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D040502:Calgranulin B / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D006801:Humans; D006965:Hyperplasia; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D010519:Periodontium / Q000382:microbiology; D016966:Porphyromonas gingivalis; D017539:Tunica Intima / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D015854:Up-Regulation
chemical_list: D040502:Calgranulin B