Fluorescence cross-correlation spectroscopy of plant proteins.
Fluorescence cross-correlation spectroscopy (FCCS) is a technique that physically evaluates the molecular interaction between two fluorophore-tagged molecules such as proteins and oligonucleotides in a quantitative manner. Because it simply makes use of the coincidental movement of two molecules, it could avoid the complexity that sometimes occurs in other fluorescent techniques such as fluorescence resonance energy transfer. The present chapter describes procedures for FCCS of plant proteins expressed and measured in HeLa cells. Determination in plant cells is also mentioned briefly.
Muto
Hideki
H
Laboratory of Supramolecular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan.
Kinjo
Masataka
M
Yamamoto
Kotaro T
KT
D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein