The role of AmpR in regulation of L1 and L2 beta-lactamases in Stenotrophomonas maltophilia.
Stenotrophomonas maltophilia is known to produce at least two chromosomal-mediated inducible beta-lactamases, L1 and L2. Gene L2, which encodes a class A beta-lactamase, and the adjacent ampR gene form an ampR-class A beta-lactamase module. L1 belongs to the class B beta-lactamase and has no neighbor ampR-like regulatory gene. In this study, the ampR-L2 module from S. maltophilia KH was compared with ampR-beta-lactamase modules from several microorganisms with respect to the AmpR and beta-lactamase proteins and the intergenic (IG) region. S. maltophilia and Xanthomonas campestris showed the most closely phylogenetic relationship among the microorganisms considered. The regulatory role of AmpR towards L1 and L2 was further analyzed. In the absence of an inducer, AmpR acted as an activator for L1 expression and as a repressor for L2 expression, whereas AmpR was an activator for both genes in an induced state. In addition, inducibility of L1 and L2 genes depended on the presence of AmpR. The ampR transcript was weakly and constitutively expressed, but was not autoregulated.
Lin
Cheng-Wen
CW
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
Huang
Yi-Wei
YW
Hu
Rouh-Mei
RM
Chiang
Kai-Hung
KH
Yang
Tsuey-Ching
TC
D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D020615:Stenotrophomonas maltophilia / Q000201:enzymology* / Q000235:genetics; D015533:Transcriptional Activation; D016959:Xanthomonas campestris / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D001426:Bacterial Proteins; C071526:AmpR protein, Bacteria; C088032:beta-lactamase L1; C425104:beta-lactamase L2; D001618:beta-Lactamases