Upregulation of S100 calcium-binding protein A9 is required for induction of smooth muscle cell proliferation by a periodontal pathogen.
We investigated the effect of a periodontal pathogen, Porphyromonas gingivalis, on human aortic smooth muscle cell (hAOSMC) proliferation as mechanisms of atherosclerosis. Cultured hAOSMCs exposed to the supernatant of plasma incubated with P. gingivalis showed a marked transformation from a contractile to proliferative phenotype, resulting in enhancement of cell growth. DNA microarray analysis revealed a P. gingivalis-dependent upregulation of S100A9 in hAOSMCs. Small interference-RNA for S100A9 dramatically attenuated the effect of P. gingivalis on transformation and proliferation of hAOSMCs. Our data suggested that upregulation of S100A9 mediated by P. gingivalis is an important event in the development of aortic intimal hyperplasia.
Inaba
Hiroaki
H
Department of Oral Frontier Biology, Graduate School of Dentistry, Osaka University, Suita 565-0871, Japan.
Hokamura
Kazuya
K
Nakano
Kazuhiko
K
Nomura
Ryota
R
Katayama
Kazufumi
K
Nakajima
Atsushi
A
Yoshioka
Hideo
H
Taniguchi
Kazuhiro
K
Kamisaki
Yoshinori
Y
Ooshima
Takashi
T
Umemura
Kazuo
K
Murad
Ferid
F
Wada
Koichiro
K
Amano
Atsuo
A
D001011:Aorta / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D040502:Calgranulin B / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D006801:Humans; D006965:Hyperplasia; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D010519:Periodontium / Q000382:microbiology; D016966:Porphyromonas gingivalis; D017539:Tunica Intima / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D015854:Up-Regulation
D040502:Calgranulin B