Taxonomic study of a chromomycin-producing strain and reclassification of Streptomyces cavourensis subsp. washingtonensis as a later synonym of Streptomyces griseus.
A chromomycin-producing actinomycete, strain AP19, was isolated from a sample of faeces collected from Foping national nature reserve in China. Chemotaxonomic and morphological properties indicated that the novel isolate was a member of the genus Streptomyces. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence of the strain and on the 120-nt nucleotide variable gamma-region of this molecule revealed that it was closely related to Streptomyces griseus ISP 5236(T) and Streptomyces cavourensis subsp. washingtonensis ATCC 27732(T). DNA-DNA relatedness values among these strains were above 70 %. Streptomyces cavourensis subsp. washingtonensis could be readily distinguished from Streptomyces cavourensis ATCC 14889(T) by differing BOX-PCR fingerprinting patterns, relatively low 16S rRNA gene sequence similarity and a low DNA-DNA relatedness value. It is proposed, therefore, that Streptomyces cavourensis subsp. washingtonensis is a later synonym of Streptomyces griseus.
Wu
Xuechang
X
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, PR China.
Wen
Yanping
Y
Qian
Chaodong
C
Li
Ou
O
Fang
Haihuang
H
Chen
Weifeng
W
D002865:Chromomycins / Q000378:metabolism*; D002965:Classification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013302:Streptomyces / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D013305:Streptomyces griseus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology*
D002865:Chromomycins; D012336:RNA, Ribosomal, 16S