Differential expression profiles of microRNAs in NIH3T3 cells in response to UVB irradiation.
MicroRNAs (miRNAs) are known as a kind of small, noncoding RNA, which play an important role in mediating many biological processes such as development, cell proliferation and differentiation in plants and animals. Here we report the differential expression profiles of miRNAs and characterized putative target genes in NIH3T3 cells at a series of different time points after UVB irradiation (compared with no UVB irradiation). The relative expression of mature miRNA genes was determined by miRNA microarray technique and the results were confirmed by real-time reverse transcriptase polymerase chain reaction (qRT-PCR). Potential target genes of these miRNAs were classified into different function categories with the GOstat software (http://gostat.wehi.edu.au/cgi-bin/goStat.pl). Several miRNAs in this study expressed highly at different time points, especially mmu-miR-365 and mmu-miR-21. Three miRNAs were lowly expressed, of which mmu-miR-465 showed low levels of expression at all time points, whereas after 50 J m(-2) UVB irradiation mmu-miR-296 and mmu-miR-376c showed low levels of expression at 6 and 12 h, respectively. Our study provided a basis for the global characterization of UV-regulated miRNA expression.
Guo
Ling
L
Department of Radiation Medicine, Southern Medical University, Guangzhou, Guangdong Province, China.
Huang
Zhong-Xi
ZX
Chen
Xiao-Wei
XW
Deng
Qin-Kai
QK
Yan
Wei
W
Zhou
Mei-Juan
MJ
Ou
Cheng-Shan
CS
Ding
Zhen-Hua
ZH
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D020869:Gene Expression Profiling; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D041681:NIH 3T3 Cells; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014466:Ultraviolet Rays
D035683:MicroRNAs