title: Isolation of two C-reactive protein homologues from cod (Gadus morhua L.) serum.
abstract: Pentraxins are important molecules in innate defence and play a role in the acute phase response of both mammals and fish. Isolation of cod pentraxins by affinity chromatography using phosphorylcholine agarose revealed two pentraxin-like proteins, referred to as PI and PII proteins. These varied in their overall charge, pentameric and subunit molecular size, glycosylation and N-terminal amino acid sequences. The PI protein was homologous with the CRP-like pentraxin previously described in cod whereas the PII protein was a new CRP homologue, which was characterized by substantial individual heterogeneity with regard to subunit size and relative density. The results indicate considerable genetic variations in the cod pentraxins.
authors.affiliation: Institute for Experimental Pathology, University of Iceland, Keldur v. Vesturlandsveg, 112 Reykjavik, Iceland.
authors.forename: Berglind
authors.initials: B
authors.lastname: Gisladottir
authors.forename: Sigridur
authors.initials: S
authors.lastname: Gudmundsdottir
authors.forename: Linton
authors.initials: L
authors.lastname: Brown
authors.forename: Zophonias O
authors.initials: ZO
authors.lastname: Jonsson
authors.forename: Bergljot
authors.initials: B
authors.lastname: Magnadottir
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002097:C-Reactive Protein / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D048389:Gadus morhua / Q000097:blood* / Q000276:immunology*; D006031:Glycosylation; D008024:Ligands; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D000911:Antibodies, Monoclonal; D029941:Fish Proteins; D008024:Ligands; D002097:C-Reactive Protein