title: Rapid and sensitive quantitation of major constituents in Danggui Buxue Tang by ultra-fast HPLC-TOF/MS.
abstract: A quantitative method by ultra-fast HPLC coupled with time-of-flight mass spectrometry (TOF/MS) has been developed for analysis of 15 major constituents in Danggui Buxue Tang (DBT) preparations. Its performance was compared with a conventional HPLC method with diode array detection/evaporative light scattering detection (DAD/ESLD). Accurate mass measurements within 3ppm error were obtained for all the compounds. The analytical time by an ultra-fast system is 4 times faster than conventional HPLC, the limits of detection by TOF/MS are low to 0.004-0.08ng compared with 1-200ng for HPLC-DAD-ELSD, and acceptable linearity of response was demonstrated over two orders of magnitude (r(2)>0.99) for all analytes. Intra-day reproducibility was below 3% and inter-day values were below 5% R.S.D. Robustness and applicability of the method was validated for the analysis of market DBT real samples, proposing a rapid, sensitive and validated method for routine analysis and quality control of DBT.
authors.affiliation: Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Wang
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010316:Particle Size; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D016062:Porosity; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012997:Solvents; C477279:danggui buxue decoction