Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China.
A Gram-positive, halophilic bacterium was isolated from a sediment sample from Ai-Ding salt lake in China. The isolate, designated strain 17-5(T), grew at salinities of 8-33 % (w/v) NaCl (optimally at 12 %, w/v). The genomic DNA G+C content of strain 17-5(T) was 48.1 mol%. The predominant isoprenoid quinone was MK-7(H(2)) and the cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(16 : 0) and C(16 : 0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 17-5(T) was a member of the genus Bacillus, being most closely related to Bacillus qingdaonensis JCM 14087(T) (96.0 % sequence similarity) and Bacillus salarius DSM 16461(T) (95.6 %). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species were less than 91.7 %. Comparative analysis of the 16S rRNA gene sequence data, chemotaxonomy and phenotypic features of the novel isolate and related species of Bacillus indicated that strain 17-5(T) represents a novel species within the genus Bacillus, for which the name Bacillus aidingensis sp. nov. is proposed. The type strain is 17-5(T) (=CGMCC 1.3227(T)=DSM 18341(T)).
Xue
Yanfen
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Ventosa
A
A
Wang
Xiaowei
X
Ren
Peigen
P
Zhou
Peijin
P
Ma
Yanhe
Y
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D002681:China; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D012623:Seawater / Q000382:microbiology*; D013045:Species Specificity
D012336:RNA, Ribosomal, 16S; D012492:Salts