title: Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: results of the 2006-2007 EDNAP collaborative exercise.
abstract: The European DNA Profiling (EDNAP) Group performed a collaborative exercise on a mitochondrial (mt) DNA screening assay that targeted 16 nucleotide positions in the coding region and allowed for the discrimination of major west Eurasian mtDNA haplogroups. The purpose of the exercise was to evaluate the stability and reproducibility of the self-developed multiplex-PCR and multiplex-single base extension kit by blind-testing saliva and hair shaft samples provided by the organizing laboratory. The overall success rate in obtaining useful results was high given that some of the participating laboratories had no previous experience with the technology and/or mtDNA analysis. The results of this collaborative exercise stimulate the expansion of screening methods in forensic laboratories to increase efficiency and performance of mtDNA typing, and thus demonstrates that mtDNA SNP typing is a powerful tool for forensic casework analysis.
authors.affiliation: Institute of Legal Medicine, Innsbruck Medical University, Austria. walther.parson@i-med.ac.at
authors.forename: Walther
authors.initials: W
authors.lastname: Parson
authors.forename: Liane
authors.initials: L
authors.lastname: Fendt
authors.forename: David
authors.initials: D
authors.lastname: Ballard
authors.forename: Claus
authors.initials: C
authors.lastname: Børsting
authors.forename: Bernd
authors.initials: B
authors.lastname: Brinkmann
authors.forename: Angel
authors.initials: A
authors.lastname: Carracedo
authors.forename: Mónica
authors.initials: M
authors.lastname: Carvalho
authors.forename: Michael D
authors.initials: MD
authors.lastname: Coble
authors.forename: Francisco Corte
authors.initials: FC
authors.lastname: Real
authors.forename: Stijn
authors.initials: S
authors.lastname: Desmyter
authors.forename: Berit M
authors.initials: BM
authors.lastname: Dupuy
authors.forename: Cheryl
authors.initials: C
authors.lastname: Harrison
authors.forename: Carsten
authors.initials: C
authors.lastname: Hohoff
authors.forename: Rebecca
authors.initials: R
authors.lastname: Just
authors.forename: Tanja
authors.initials: T
authors.lastname: Krämer
authors.forename: Niels
authors.initials: N
authors.lastname: Morling
authors.forename: Antonio
authors.initials: A
authors.lastname: Salas
authors.forename: Hermann
authors.initials: H
authors.lastname: Schmitter
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schneider
authors.forename: Marie-Luise
authors.initials: ML
authors.lastname: Sonntag
authors.forename: Peter M
authors.initials: PM
authors.lastname: Vallone
authors.forename: Anita
authors.initials: A
authors.lastname: Brandstätter
mesh_terms: D016009:Chi-Square Distribution; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D005554:Forensic Medicine / Q000379:methods; D005819:Genetic Markers; D005828:Genetics, Population; D006197:Hair / Q000737:chemistry; D006239:Haplotypes; D006801:Humans; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012463:Saliva / Q000737:chemistry; D044465:White People / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers