Excellent combination of counter-current chromatography and preparative high-performance liquid chromatography to separate galactolipids from pumpkin.
Galactolipids in the fruits of Cucurbita moschata (pumpkin) could not be completely separated by high-performance liquid chromatography (HPLC). Preparative HPLC was not available for preparing major galactolipid monomers in pumpkin. In the present paper, a combination of high-speed counter-current chromatography (HSCCC) and preparative HPLC was used for preparing the galactolipids. A fraction containing galactolipids (Fr60) from the purification of the n-butanol extract of pumpkin by macro-porous resin column chromatography was first separated by HSCCC to result in three sub-fractions of each containing two galactolipid monomers. The three sub-fractions were further separated by preparative HPLC respectively to yield six galactolipid monomers with purity more than 96%. The method is a good one for preparing galactolipid monomers from plant materials for the studies of bioactivities.
Du
Qizhen
Q
Institute of Food and Biological Engineering, Zhejiang Gongshang University, Hangzhou, China. qizhendu@163.com
Jiang
Zhiguo
Z
Wang
Daijie
D
D020001:1-Butanol / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution / Q000379:methods*; D028464:Cucurbita / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D038983:Galactolipids / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
D000097:Acetonitriles; D038983:Galactolipids; D012997:Solvents; D020001:1-Butanol; C032159:acetonitrile