GM-CSF-mediated T-cell activation by macrophages infected with recombinant BCG that secretes major membrane protein-II of Mycobacterium leprae.
The potential of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) needs to be augmented to efficiently activate CD4(+) T cells through macrophages. Mycobacterium leprae-derived recombinant major membrane protein (MMP)-II induced GM-CSF production from macrophages. A recombinant BCG-SM that secretes MMP-II more efficiently produced GM-CSF and activated interferon (IFN)-gamma-producing CD4(+) T cells than did vector control BCG when infected with macrophages. The T-cell activation by BCG-SM was dependent on the GM-CSF production by macrophages. Interleukin (IL)-10 production by macrophages stimulated with M. leprae was inhibited in a GM-CSF-dependent manner when the precursor monocytes were infected with BCG-SM. BCG inducing GM-CSF production was effective in macrophage-mediated T-cell activation partially through IL-10 inhibition.
Makino
Masahiko
M
Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan. mmaki@nih.go.jp
Maeda
Yumi
Y
Kai
Masanori
M
Tamura
Toshiki
T
Mukai
Tetsu
T
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002675:Child, Preschool; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016753:Interleukin-10 / Q000276:immunology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*
D008565:Membrane Proteins; C500901:major membrane protein II, Mycobacterium leprae; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor