Cell survival within pulp and periodontal constructs.
The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs (n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria. The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments.
Gebhardt
Matthew
M
Department of Endodontics, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328-2018, USA.
Murray
Peter E
PE
Namerow
Kenneth N
KN
Kuttler
Sergio
S
Garcia-Godoy
Franklin
F
D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D002130:Calcium Phosphates; D002417:Cattle; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D007739:L Cells; D019344:Lactic Acid; D008422:Materials Testing; D051379:Mice; D010513:Periodontal Ligament / Q000166:cytology*; D011091:Polyesters; D011108:Polymers; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; C020243:calcium phosphate