title: Biofilm formation of Candida albicans on the surface of a soft denture-lining material.
abstract: BACKGROUND
  Soft denture lining-materials are more susceptible to microbial adhesion than hard denture base acrylic resin. Poor oral hygiene and Candida albicans infection are common among elderly denture wearers as these patients usually have difficulty in keeping them clean.
  
  
  OBJECTIVE
  To evaluate the influence of the oral hygiene methods on the formation of a biofilm over a soft denture-lining material.
  
  
  METHODS
  Twenty volunteers were randomly separated into two groups: G1 and G2. Ten volunteers performed daily hygiene of the prostheses with a soft toothbrush and toothpaste. The G2 performed a treatment identical to G1 but also immersed the prostheses in sodium hypochlorite 0.5% for 20 min, once a week. Quantification of the mean score values of biofilm formation at different times were statistically analysed using analysis of variance and Tukey's test (alpha = 0.05).
  
  
  RESULTS
  G1 (0.65 +/- 0.52) showed the lowest mean score values of biofilm formation. There was statistical difference between G1 and G2. The highest mean score values were found at 6 weeks (1.3 +/- 1.08) and were statistically different from other times.
  
  
  CONCLUSIONS
  The oral hygiene methods had a significant effect in the formation of the biofilm over a soft denture-lining material.
authors.affiliation: Department of Prosthodontics, School of Dentistry, West University of Santa Catarina, Santa Catarina, Brazil. noeli@boscatto.com.br
authors.forename: Noéli
authors.initials: N
authors.lastname: Boscato
authors.forename: Alessandra
authors.initials: A
authors.lastname: Radavelli
authors.forename: Daniela
authors.initials: D
authors.lastname: Faccio
authors.forename: Alessandro Dourado
authors.initials: AD
authors.lastname: Loguercio
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D003774:Dental Plaque Index; D003820:Denture Cleansers / Q000627:therapeutic use; D003822:Denture Liners / Q000382:microbiology*; D003827:Denture, Complete, Upper / Q000382:microbiology*; D004202:Disinfectants / Q000627:therapeutic use*; D006801:Humans; D007575:Jaw, Edentulous / Q000382:microbiology; D008437:Maxilla; D009910:Oral Hygiene / Q000379:methods; D016037:Single-Blind Method; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D014099:Toothbrushing
chemical_list: D003820:Denture Cleansers; D004202:Disinfectants; D012973:Sodium Hypochlorite