title: Primate-specific spliced PMCHL RNAs are non-protein coding in human and macaque tissues.
abstract: BACKGROUND
  Brain-expressed genes that were created in primate lineage represent obvious candidates to investigate molecular mechanisms that contributed to neural reorganization and emergence of new behavioural functions in Homo sapiens. PMCHL1 arose from retroposition of a pro-melanin-concentrating hormone (PMCH) antisense mRNA on the ancestral human chromosome 5p14 when platyrrhines and catarrhines diverged. Mutations before divergence of hylobatidae led to creation of new exons and finally PMCHL1 duplicated in an ancestor of hominids to generate PMCHL2 at the human chromosome 5q13. A complex pattern of spliced and unspliced PMCHL RNAs were found in human brain and testis.
  
  
  RESULTS
  Several novel spliced PMCHL transcripts have been characterized in human testis and fetal brain, identifying an additional exon and novel splice sites. Sequencing of PMCHL genes in several non-human primates allowed to carry out phylogenetic analyses revealing that the initial retroposition event took place within an intron of the brain cadherin (CDH12) gene, soon after platyrrhine/catarrhine divergence, i.e. 30-35 Mya, and was concomitant with the insertion of an AluSg element. Sequence analysis of the spliced PMCHL transcripts identified only short ORFs of less than 300 bp, with low (VMCH-p8 and protein variants) or no evolutionary conservation. Western blot analyses of human and macaque tissues expressing PMCHL RNA failed to reveal any protein corresponding to VMCH-p8 and protein variants encoded by spliced transcripts.
  
  
  CONCLUSIONS
  Our present results improve our knowledge of the gene structure and the evolutionary history of the primate-specific chimeric PMCHL genes. These genes produce multiple spliced transcripts, bearing short, non-conserved and apparently non-translated ORFs that may function as mRNA-like non-coding RNAs.
authors.affiliation: Université de Nice-Sophia Antipolis, CNRS, Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France. schmieder@ipmc.cnrs.fr
authors.forename: Sandra
authors.initials: S
authors.lastname: Schmieder
authors.forename: Fleur
authors.initials: F
authors.lastname: Darré-Toulemonde
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Arguel
authors.forename: Audrey
authors.initials: A
authors.lastname: Delerue-Audegond
authors.forename: Richard
authors.initials: R
authors.lastname: Christen
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Nahon
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D019143:Evolution, Molecular; D005091:Exons; D006801:Humans; D007028:Hypothalamic Hormones / Q000235:genetics*; D007438:Introns; D008251:Macaca / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates; D011498:Protein Precursors / Q000235:genetics*; D012326:RNA Splicing; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013737:Testis / Q000378:metabolism
chemical_list: D007028:Hypothalamic Hormones; D011498:Protein Precursors; D022661:RNA, Untranslated; C059991:melanin-concentrating hormone precursors