A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region.
The analysis of X-STR polymorphisms has received the attention of several researchers, mainly due to its applicability to the investigation of complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in different populations of the world. The present work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. The loci investigated include DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011, which together afford a power of discrimination in the order of 0.999999. In addition, this work presents the genotyping results obtained for a sample of 324 individuals (182 males and 142 females) from the admixed population of Belém, Pará, located in the Brazilian Amazon Region.
Ribeiro Rodrigues
Elzemar Martins
EM
Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil.
Leite
Fabio Pereira das Neves
FP
Hutz
Mara Helena
MH
Palha
Teresinha de Jesus Brabo Ferreira
Tde J
Ribeiro dos Santos
Andrea Kely Campos
AK
dos Santos
Sidney Emanuel Batista
SE
D001938:Brazil; D041321:Chromosomes, Human, X; D005260:Female; D053803:Forensic Genetics; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction; D045483:Rivers
D005819:Genetic Markers