[Effect of microRNA143 expression on cell proliferation in colonic carcinoma].
OBJECTIVE
To investigate the effect of microRNA143 on cell proliferation and K-ras expression in colorectal carcinoma.


METHODS
Northern blot was used to examine the expression of miR-143 in colorectal carcinoma and adjacent normal tissues. A miR-143 expression vector was constructed and transfected into a human colon adenocarcinoma cell line SW480. Cell proliferation was evaluated by MTT assay. RT-PCR and Western blot were used to examine the expression of K-ras oncogene in transfected cells.


RESULTS
The level of mature miR-143 was lower in tumors compared with adjacent normal tissues in 81% of colorectal carcinoma specimens. In transfected cells, the increased accumulation of miR-143 inhibited the cell proliferation, and resulted in approximately 40.3% decrease of K-ras protein levels, but had no effect on level of K-ras mRNA.


CONCLUSIONS
The increased accumulation of miR-143 inhibits the proliferation of transfected cells, and results in down-regulation of K-ras protein in colorectal carcinoma.
Liu
Hong
H
Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
Zhang
Su-Zhan
SZ
Cai
Shan-Rong
SR
Peng
Jia-Ping
JP
Zheng
Shu
S
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D011905:Genes, ras; D005822:Genetic Vectors; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D018631:ras Proteins / Q000378:metabolism*
C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D018631:ras Proteins