Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm.
Seventy isolates were obtained from a marine electroactive biofilm that was generated on a cathodically polarized stainless steel electrode (Genoa, Italy). The genetic diversity was investigated by means of BOX-PCR fingerprinting and two clusters of isolates with similar BOX-PCR profiles were delineated. Whole-cell fatty acid methyl ester analysis and 16S rRNA gene sequence analysis showed that the isolates belonged to the Roseobacter lineage of the class Alphaproteobacteria. DNA-DNA hybridization experiments and a biochemical analysis demonstrated that four isolates belonged to the species Ruegeria mobilis. However, 66 isolates from the second BOX-PCR cluster constituted a novel species within the genus Ruegeria, for which the name Ruegeria scottomollicae sp. nov. is proposed. The DNA G+C content was 61.0+/-0.4 %. The type strain is LMG 24367(T) (=CCUG 55858(T)).
Vandecandelaere
Ilse
I
Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, Gent, Belgium. Ilse.Vandecandelaere@UGent.be
Nercessian
Olivier
O
Segaert
Eveline
E
Achouak
Wafa
W
Faimali
Marco
M
Vandamme
Peter
P
D020561:Alphaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D018441:Biofilms; D004560:Electricity; D004566:Electrodes / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D008386:Marine Biology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S