title: Expression and functional role of MDL-1 (CLEC5A) in mouse myeloid lineage cells.
abstract: Myeloid DNAX activation protein 12 (DAP12)-associating lectin-1 (MDL-1), also known as C-type lectin domain family 5, member A, is a type II transmembrane protein belonging to the C-type lectin family and associates with DAP12 (also called KARAP or TYROBP). It has been reported that two isoforms of MDL-1-long form (MDL-1L) and short form (MDL-1S)-exist in mice. Previously, we observed the marked induction of MDL-1 mRNA expression during the pulmonary mycobacterial infection in mice. The data suggested that the MDL-1-expressing cells were involved in immune responses against mycobacterial infection; however, little is known about the function of MDL-1 as yet. In this study, we demonstrated the significant protein expression of MDL-1L and MDL-1S in mouse neutrophils and macrophages. MDL-1L was highly glycosylated by N-linked glycan and sialic acid. Interestingly, the expression pattern of MDL-1 was different between neutrophils and macrophages. MDL-1 expression was notably induced during the differentiation of the mouse myeloid cell line 32Dcl3 into neutrophils. Additionally, we observed that MDL-1 stimulation induced a significant amount of RANTES and macrophage-derived chemokine production in 32Dcl3 cells in cooperation with signaling through TLR. MDL-1 stimulation also up-regulated CD11b expression and maintained cell survival. Our findings indicate that MDL-1, therefore, plays an important role in immune defense as a result of an innate immunity, which involves neutrophils and macrophages.
authors.affiliation: Asahikawa Medical College, Midorigaoka-higashi 2-1-1-1, Asahikawa, Japan. aoq@asahikawa-med.ac.jp.
authors.forename: Naoko
authors.initials: N
authors.lastname: Aoki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kimura
authors.forename: Shoji
authors.initials: S
authors.lastname: Kimura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagato
authors.forename: Makoto
authors.initials: M
authors.lastname: Azumi
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sato
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tateno
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D018925:Chemokines / Q000096:biosynthesis; D006031:Glycosylation; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000235:genetics / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology*; D009504:Neutrophils / Q000378:metabolism*; D020033:Protein Isoforms; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*
chemical_list: D018925:Chemokines; C509772:Clec5a protein, mouse; D037181:Lectins, C-Type; D020033:Protein Isoforms; D011956:Receptors, Cell Surface