Measurement of within and between genetic variability in duck breeds by RAPD markers.
A total of 200 genomic DNAs were isolated from the four breeds of duck: Muscovy, Pekin, Khaki Campbell and Native, through a modified salting out procedure. The samples were used in a Polymerase Chain Reaction (PCR) with 27 RAPD markers. Amplified PCR-products with the markers were separated on a 2% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands were described. Genetic similarity and genetic distance were calculated. The RAPD analysis data from 7 primers were utilized in estimating genetic similarity between four breeds, which ranged from 0.46 to 0.77 between the breeds. The maximum genetic distance was observed between Muscovy and Native duck breeds (0.76).
Gholizadeh
Mohsen
M
Lab. For Molecular Genetic and Animal Biotechnology, Department of Animal Science, Faculty of Agricultural Science, Mazandaran University, P.O. Box 578, Sari, Iran.
Mianji
Ghodrat Rahimi
GR
Ghobadi
Abbas
A
Zadeh
Hadi Sayyah
HS
Montazami
Shahab
S
D000818:Animals; D016000:Cluster Analysis; D004372:Ducks / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity