Optimization of headspace solid phase microextraction procedure for trace analysis of toluene.
This study describes optimization of headspace solid phase microextraction followed by gas chromatography equipped with a flame ionization detector for toluene at trace level in spiked urine. The parameters affecting the extraction and gas chromatographic determination of analytes were studied: extraction time and temperature; desorption time and temperature; addition of NaCl; and pH, volume and agitation of the sample. Optimized headspace extraction was carried out at 30 degrees C for 6 min in the presence of 0.2 g.m(-1)of NaCl in the sample solution. Also, sample volume and sample pH were optimized at 5 ml and 7 (neutral pH), respectively. Desorption of the analytes was carried out at 250 degrees C for 60 s. The optimized procedure was validated with 3 different pools of spiked urine; it showed good reproducibility over 6 consecutive days and 6 within-day experiments. The study also determined the accuracy, linearity and detection limits of this method.
Heidari
Hamid-Reza
HR
School of Public Health & Institute of Public Health Research, Center for Environmental Research, Tehran University of Medical Sciences. IR Iran. shahtaheri@tums.ac.ir
Shahtaheri
Seyed Jamaleddin
SJ
Golbabaei
Farideh
F
Alimohammadi
Mahmoud
M
Rahimi-Froushani
Abbas
A
D002849:Chromatography, Gas / Q000592:standards*; D006801:Humans; D052617:Solid Phase Microextraction; D014050:Toluene / Q000032:analysis* / Q000652:urine
D014050:Toluene