title: Ribotyping and whole-cell protein analysis of spirochetes isolated from arthropods in the Czech Republic.
abstract: In the presented work, six Borrelia burgdorferi sensu lato isolates were recovered from Ixodes ricinus ticks and one strain of spirochete with uncertain taxonomic position isolated from Culex pipiens mosquito collected in the Valtice area (South Moravia, Czech Republic), as well as representative type strains, were further characterized by ribotyping and whole-cell protein fingerprinting. The ribotype patterns obtained with HindII and EcoRV ribotype patterns showed good correlations with the recently proposed genotypes and grouped each genospecies in a well-separated cluster. The whole-cell protein profiles of genospecies were more heterogeneous than the ribotype patterns. The non-identified spirochetal strain was clearly separated from the Borrelia isolates in both methods thus proving their good capacity to characterize Borrelia burgdorferi sensu lato strains.
authors.affiliation: Department of Lipids, Tensides and Cosmetics Technology, Faculty of Technology, Tomas Bata University-Zlin, Namesti T. G. Masaryka 275, Zlin, Czech Republic. bunkova@ft.utb.cz
authors.forename: Leona
authors.initials: L
authors.lastname: Bunková
authors.forename: Pavel
authors.initials: P
authors.lastname: Svec
authors.forename: Jirí
authors.initials: J
authors.lastname: Halouzka
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
authors.forename: Miroslav
authors.initials: M
authors.lastname: Nemec
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003465:Culex / Q000382:microbiology; D018153:Czech Republic / Q000453:epidemiology; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000453:epidemiology; D010802:Phylogeny; D021521:Ribotyping
chemical_list: D001426:Bacterial Proteins