title: Functional differentiation of tbf1 orthologues in fission and budding yeasts.
abstract: In Saccharomyces cerevisiae, TBF1, an essential gene, influences telomere function but also has other roles in the global regulation of transcription. We have identified a new member of the tbf1 gene family in the mammalian pathogen Pneumocystis carinii. We demonstrate by transspecies complementation that its ectopic expression can provide the essential functions of Schizosaccharomyces pombe tbf1 but that there is no rescue between fission and budding yeast orthologues. Our findings indicate that an essential function of this family of proteins has diverged in the budding and fission yeasts and suggest that effects on telomere length or structure are not the primary cause of inviability in S. pombe tbf1 null strains.
authors.affiliation: Institute of Microbiology, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.
authors.forename: Moira M
authors.initials: MM
authors.lastname: Cockell
authors.forename: Libera
authors.initials: L
authors.lastname: Lo Presti
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cerutti
authors.forename: Elena
authors.initials: E
authors.lastname: Cano Del Rosario
authors.forename: Philippe M
authors.initials: PM
authors.lastname: Hauser
authors.forename: Viesturs
authors.initials: V
authors.lastname: Simanis
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010641:Phenotype; D045363:Pneumocystis carinii / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004718:Saccharomycetales / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D016615:Telomere / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C079376:TBF1 protein, S cerevisiae; C538990:TBF1 protein, S pombe; D014157:Transcription Factors