title: Studies on the antimicrobial effects of Spondias mombin and Baphia nittida on dental caries organism.
abstract: The antimicrobial effect of cold water, hot water and ethanolic extracts of Spondias mombin and Baphia nittida on cariogenic streptococci isolated from dental caries patients attending the Ebonyi State University Teaching hospital dental clinic Abakaliki was investigated using the agar well diffusion technique. The cold water and ethanolic extracts of Baphia nittida showed inhibition zone diameter (IZD) of 10 and 12 mm respectively at 400 mg mL(-1), while the hot water showed no inhibitory effect. All extracts of Spondias mombin did not inhibit the test organism. The cold water and ethanolic extracts of Baphia nittida showed Minimum Inhibitory Concentration (MIC) of 100 and 50 mg mL(-1) respectively. The combination of the cold water extracts of the two herbs showed enhanced activity of 13 mm. Phytochemical analysis of Baphia nittida revealed the presence of flavonoids, glycosides, proteins saponins, tannins, carbohydrate and steroidal aglycone. Acute toxicity testing of Baphia nittida at a range of 250-5000 mg kg(-1) bw using mice showed no clinical signs of acute toxicity. No chemical toxicity was observed amongst rats given Baphia nittida extracts 500 and 1000 mg kg(-1) bw after 30 days. Baphia nittida may be a potential source of an antimicrobial agent for the treatment and management of dental caries.
authors.affiliation: Department of Applied Microbiology, Faculty of Applied and Natural Sciences, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria.
authors.forename: E S
authors.initials: ES
authors.lastname: Amadi
authors.forename: A
authors.initials: A
authors.lastname: Oyeka
authors.forename: R A
authors.initials: RA
authors.lastname: Onyeagba
authors.forename: I
authors.initials: I
authors.lastname: Okoli
authors.forename: O C
authors.initials: OC
authors.lastname: Ugbogu
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D003731:Dental Caries / Q000382:microbiology* / Q000628:therapy*; D007887:Fabaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013291:Streptococcus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts