title: Successful ovarian autotransplant with no vascular reanastomosis in rats.
abstract: Preservation of ovarian functions in woman with premature ovarian failure remains an issue in reproductive medicine. Hormone replacement therapy for maintaining endocrine functions, and cryopreservation of embryos or oocytes for those who wish pregnancy, are some of the choices. However, ovarian transplantation is a more physiological alternative, although problems related to ovarian ischemia have been reported. Herein, we investigated the viability of autologous transplantation of the ovarian tissue into the rat peritoneum, without vascular reanastomosis. Twenty animals in the study group had both ovaries excised, and each ovary was dissected into two halves. A half of an ovary was autotransplanted to the peritoneal surface, closely located to the left epigastric vessels. This simple procedure does not require surgical vascular reanastomosis while it maintains appropriate follicular growth and therefore should be further considered as an alternative for women undergoing oophorectomy, not only to maintain endocrine functions but also for fertility preservation.
authors.affiliation: Medical School of the Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Flávio S V
authors.initials: FS
authors.lastname: Barros
authors.forename: Rodrigo M
authors.initials: RM
authors.lastname: de Oliveira
authors.forename: Felipe M T
authors.initials: FM
authors.lastname: Alves
authors.forename: Marcos
authors.initials: M
authors.lastname: Sampaio
authors.forename: Selmo
authors.initials: S
authors.lastname: Geber
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D005298:Fertility; D006085:Graft Survival; D009865:Oocytes / Q000378:metabolism; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology / Q000637:transplantation*; D016649:Primary Ovarian Insufficiency / Q000628:therapy*; D051381:Rats; D012044:Regression Analysis; D014180:Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D016896:Treatment Outcome