title: Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers.
abstract: Streptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.
authors.affiliation: Department of Physiological Science, Dental School of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil. cinthia@fop.unicamp.br
authors.forename: Cínthia Pereira Machado
authors.initials: CP
authors.lastname: Tabchoury
authors.forename: Maria Clara K
authors.initials: MC
authors.lastname: Sousa
authors.forename: Rodrigo Alex
authors.initials: RA
authors.lastname: Arthur
authors.forename: Renata Oliveira
authors.initials: RO
authors.lastname: Mattos-Graner
authors.forename: Altair Antoninha
authors.initials: AA
authors.lastname: Del Bel Cury
authors.forename: Jaime Aparecido
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005456:Fluorescent Dyes; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D004996:Ethidium