Genome-wide analysis of genetic changes in intestinal-type sinonasal adenocarcinoma.
BACKGROUND
Intestinal-type sinonasal adenocarcinomas are rare tumors related to professional exposure to wood dust. Little is known about the genetic changes in these tumors.


METHODS
Twenty-two tumors were analyzed by microarray comparative genomic hybridization (CGH). In addition, DNA ploidy was measured by flow cytometry and microsatellite instability (MSI) by multiplex PCR.


RESULTS
The most frequent gains were, in descending order, as follows: 5p15, 20q13, and 8q24. Losses occurred most frequently at 4q31-qter, 18q12-22, 8p12-pter, and 5q11-qter. MSI was not detected. Seven cases that harbored very few changes were mostly DNA diploid and had more favorable clinicopathological features, such as lack of intracranial invasion, less metastases, and longer overall survival.


CONCLUSIONS
The microarray CGH results enabled to better define hotspots of chromosomal gains and losses for further investigation of genes involved in the tumorigenesis of sinonasal adenocarcinoma. In addition, the data allowed classification of a group of patients with better clinical outcome.
Hermsen
Mario A
MA
Department of Otorhinolaryngology, IUOPA, Hospital Universitario Central de Asturias, Asturias, Spain. mhermsen@hca.es
Llorente
José Luis
JL
Pérez-Escuredo
Jhudit
J
López
Fernando
F
Ylstra
Bauke
B
Alvarez-Marcos
César
C
Suárez
Carlos
C
D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D004391:Dust; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010255:Paranasal Sinus Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011003:Ploidies; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D014934:Wood / Q000009:adverse effects
D004391:Dust