title: Direct electrochemistry and electrocatalysis of hemoglobin in gelatine film modified glassy carbon electrode.
abstract: Direct electrochemical and electrocatalytic behavior of hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containing gelatine (Gel) films was investigated. The characteristics of Hb/Gel film modified GC electrode were performed by using SEM microscopy, UV-vis spectroscopy and electrochemical methods. The immobilized Hb showed a couple of quasi-reversible redox peak with a formal potential of -0.38V (versus SCE) in 0.1M pH 7.0 PBS. The formal potential changed linearly from pH 4.03 to 8.41 with a slope value of -52.0mV pH(-1), which suggested that a proton transfer was accompanied with each electron transfer (ET) in the electrochemical reaction. The Hb/gelatine/GCE displayed a rapid amperometric response to the reduction of H(2)O(2) and nitrite.
authors.affiliation: Key Lab. of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Jin-Zhong
authors.initials: JZ
authors.lastname: Xu
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu