title: Molecular determinants of the regioselectivity of toluene/o-xylene monooxygenase from Pseudomonas sp. strain OX1.
abstract: Bacterial multicomponent monooxygenases (BMMs) are a heterogeneous family of di-iron monooxygenases which share the very interesting ability to hydroxylate aliphatic and/or aromatic hydrocarbons. Each BMM possesses defined substrate specificity and regioselectivity which match the metabolic requirements of the strain from which it has been isolated. Pseudomonas sp. strain OX1, a strain able to metabolize o-, m-, and p-cresols, produces the BMM toluene/o-xylene monooxygenase (ToMO), which converts toluene to a mixture of o-, m-, and p-cresol isomers. In order to investigate the molecular determinants of ToMO regioselectivity, we prepared and characterized 15 single-mutant and 3 double-mutant forms of the ToMO active site pocket. Using the Monte Carlo approach, we prepared models of ToMO-substrate and ToMO-reaction intermediate complexes which allowed us to provide a molecular explanation for the regioselectivities of wild-type and mutant ToMO enzymes. Furthermore, using binding energy values calculated by energy analyses of the complexes and a simple mathematical model of the hydroxylation reaction, we were able to predict quantitatively the regioselectivities of the majority of the variant proteins with good accuracy. The results show not only that the fine-tuning of ToMO regioselectivity can be achieved through a careful alteration of the shape of the active site but also that the effects of the mutations on regioselectivity can be quantitatively predicted a priori.
authors.affiliation: Dipartimento di Biologia Strutturale e Funzionale, Universitá di Napoli Federico II, Complesso Universitario di Monte S. Angelo, Naples, Italy. notomist@unina.it
authors.forename: Eugenio
authors.initials: E
authors.lastname: Notomista
authors.forename: Valeria
authors.initials: V
authors.lastname: Cafaro
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bozza
authors.forename: Alberto
authors.initials: A
authors.lastname: Di Donato
mesh_terms: D020134:Catalytic Domain; D003408:Cresols / Q000378:metabolism; D004252:DNA Mutational Analysis; D007700:Kinetics; D008958:Models, Molecular; D008962:Models, Theoretical; D020125:Mutation, Missense; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics*; D011549:Pseudomonas / Q000201:enzymology*; D013237:Stereoisomerism; D013379:Substrate Specificity; D014050:Toluene / Q000378:metabolism
chemical_list: D003408:Cresols; D014050:Toluene; D010105:Oxygenases; C114360:toluene 2-xylene monooxygenase