High performance liquid chromatography/electron spin resonance/mass spectrometry analyses of lipid-derived radicals.
High performance liquid chromatography/electron spin resonance/mass spectrometry (HPLC/ EPR/MS) analyses of radicals is performed for the reaction mixture of 13-hydroperoxy-(9Z,11E)-octadeca-9,11-dienoic acid (13ZE-OOH) with ferrous ions under an aerobic condition, or an anaerobic condition. Radicals are identified from 13ZE-OOH by using high performance liquid chromatography/electron spin resonance spectrometry (HPLC/EPR) and HPLC/EPR/MS. The pentyl radical and isomers of epoxylinoleic acid radicals from 13ZE-OOH are identified under an anaerobic condition and the 7-carboxyheptyl radical and pentyl radical from 13ZE-OOH under an aerobic condition. These results suggest that the formation of the respective radical species depends to a great extent on oxygen concentration in the reaction mixtures.
Iwahashi
Hideo
H
Department of Chemistry, Wakayama Medical University, Wakayama, Japan.
D000332:Aerobiosis; D000693:Anaerobiosis; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000032:analysis* / Q000737:chemistry; D007501:Iron; D008054:Lipid Peroxides / Q000032:analysis / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*
D005609:Free Radicals; D008054:Lipid Peroxides; D008055:Lipids; D007501:Iron