Production of polyclonal antibodies to a recombinant coat protein of potato virus Y.
The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.
Folwarczna
J
J
Intitute of Experimental Botany, Academy of Sciences of the Czech Republic, 160 00, Prague, Czech Republic.
Plchová
H
H
Moravec
T
T
Hoffmeisterová
H
H
Dedic
P
P
Cerovská
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D015153:Blotting, Western; D036022:Capsid Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology*; D011198:Solanum tuberosum / Q000821:virology; D014026:Nicotiana / Q000821:virology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C090960:coat protein, Potato virus Y