Resveratrol attenuates oxidative stress and histological alterations induced by liver ischemia/reperfusion in rats.
OBJECTIVE
To investigate the effects of resveratrol on liver ischemia/reperfusion (I/R) injury in rats.


METHODS
A total of 40 male Sprague-Dawley rats weighing 240-290 g were randomized into four groups of ten: (1) controls: data from unmanipulated animals; (2) sham group: rats subjected to the surgical procedure, except for liver I/R, and given saline; (3) I/R group: rats underwent liver ischemia for 45 min followed by reperfusion for 45 min; (4) I-R/Resveratrol group: rats pretreated with resveratrol (10 micromol/L, iv). Liver tissues were obtained to determine antioxidant enzyme levels and for biochemical and histological evaluation.


RESULTS
Plasma aminotransferase activities were higher in the I/R group than in the I-R/Resveratrol group. Malondialdehyde levels and the hepatic injury score decreased, while superoxide dismutase, catalase, and glutathione peroxidase levels increased in group 4 compared to group 3. In group 4, histopathological changes were significantly attenuated in resveratrol-treated livers.


CONCLUSIONS
These results suggest that resveratrol has protective effects against hepatic I/R injury, and is a potential therapeutic drug for ischemia reperfusion-related liver injury.
Gedik
Ercan
E
Department of General Surgery, Medical School, Dicle University, Diyarbakir 21280, Turkey. ercan.gedik@yahoo.com.tr
Girgin
Sadullah
S
Ozturk
Hayrettin
H
Obay
Basra-Deniz
BD
Ozturk
Hulya
H
Buyukbayram
Huseyin
H
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D008026:Ligation; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D008107:Liver Diseases / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications* / Q000517:prevention & control; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D013267:Stilbenes; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000077185:Resveratrol