title: Immunohistochemical staining with newly developed metallothionein fragment antibodies against NH2-terminal, middle-regional and COOH-terminal peptides in rabbits.
abstract: Metallothionein (MT) has been reported to play important physiological roles in the human body, but the distribution and significance of MT is not fully understood. In order to analyze MT expression, three kinds of polyclonal MT fragment antibodies were developed against NH2-terminal, middle regional and COOH-terminal peptide followed by human MT-IA amino acid sequence in rabbits. The characteristics of these antibodies were studied using competitive immunoassay and immunohistochemical staining. The NH2-terminal antibody (anti-MT-N) had the strongest and clearest immunoreactivity to human and mouse tissues, while COOH-terminal antibody (anti-MT-C) showed species difference because of the 4 amino acid difference in the MT fragment peptide between human and mouse. No reactivity was detected using middle regional residue antibody (anti-MT-M) both on competitive immunoassay and on immunostaining. These results suggest that anti-MT-N is the most applicable antibody to use for immunohistochemistry in human, mouse and other specimens.
authors.affiliation: School of Health Sciences, Faculty of Medicine, Gunma University, Gunma, Japan. nkyoumi@health.gunma-u.ac.jp
authors.forename: Kyoumi
authors.initials: K
authors.lastname: Nakazato
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Keiji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Takeaki
authors.initials: T
authors.lastname: Nagamine
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008668:Metallothionein / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits
chemical_list: D000939:Epitopes; C530099:MT1A protein, human; D010446:Peptide Fragments; D008668:Metallothionein