Improved spiral tube assembly for high-speed counter-current chromatography.
The original spiral tube support (STS) assembly is improved by changing the shape of the tubing, with 1-cm presses perpendicularly along the length. This modification interrupts the laminar flow of the mobile phase. The tubing in the four return grooves to the center of the rotor is flattened by a specially made pressing tool to increase the number of spiral layers and decrease the dead space volume, thus increasing the column efficiency. The performance of this spiral tube assembly was tested in separations of dipeptides and proteins with suitable polar two-phase solvent systems. The results revealed that the present system yields high partition efficiency with a satisfactory level of stationary phase retention in a short elution time. The present high-speed counter-current chromatographic (HSCCC) system will be efficiently applied to a broad spectrum of two-phase solvent systems including aqueous-aqueous polymer phase systems (TPAS) which are used for separation of biopolymers such as proteins and nucleic acids.
Ito
Y
Y
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. itoy2@mail.nih.gov
Clary
R
R
Powell
J
J
Knight
M
M
Finn
T M
TM
D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods; D004151:Dipeptides / Q000302:isolation & purification; D004867:Equipment Design; D011506:Proteins / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors
D004151:Dipeptides; D011506:Proteins; D012997:Solvents