Germline transformation of Caenorhabditis elegans by injection.
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.
Kadandale
Pavan
P
Waksman Institute, Rutgers University, Piscataway, NJ, USA.
Chatterjee
Indrani
I
Singson
Andrew
A
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D012734:Disorders of Sex Development; D005854:Germ Cells / Q000378:metabolism*; D008845:Microinjections / Q000379:methods*; D014170:Transformation, Genetic