title: Radial stretch reveals distinct populations of mechanosensitive mammalian somatosensory neurons.
abstract: Primary afferent somatosensory neurons mediate our sense of touch in response to changes in ambient pressure. Molecules that detect and transduce thermal stimuli have been recently identified, but mechanisms underlying mechanosensation, particularly in vertebrate organisms, remain enigmatic. Traditionally, mechanically evoked responses in somatosensory neurons have been assessed one cell at a time by recording membrane currents in response to application of focal pressure, suction, or osmotic challenge. Here, we used radial stretch in combination with live-cell calcium imaging to gain a broad overview of mechanosensitive neuronal subpopulations. We found that different stretch intensities activate distinct subsets of sensory neurons as defined by size, molecular markers, or pharmacological attributes. In all subsets, stretch-evoked responses required extracellular calcium, indicating that mechanical force triggers calcium influx. This approach extends the repertoire of stimulus paradigms that can be used to examine mechanotransduction in mammalian sensory neurons, facilitating future physiological and pharmacological studies.
authors.affiliation: Department of Physiology, University of California, San Francisco CA 94158, USA.
authors.forename: Martha R C
authors.initials: MR
authors.lastname: Bhattacharya
authors.forename: Diana M
authors.initials: DM
authors.lastname: Bautista
authors.forename: Karin
authors.initials: K
authors.lastname: Wu
authors.forename: Henry
authors.initials: H
authors.lastname: Haeberle
authors.forename: Ellen A
authors.initials: EA
authors.lastname: Lumpkin
authors.forename: David
authors.initials: D
authors.lastname: Julius
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D040542:Mechanotransduction, Cellular; D051379:Mice; D011189:Potassium Chloride / Q000494:pharmacology; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013314:Stress, Mechanical
chemical_list: D011189:Potassium Chloride; D002118:Calcium