Re and (99m)Tc organometallic complexes containing pendant l-arginine derivatives as potential probes of inducible nitric oxide synthase.
Aiming to design radioactive compounds based on the core "(99m)Tc(CO)(3)" for probing inducible nitric oxide synthase (iNOS) levels in vivo, we have synthesized conjugates containing a pyrazolyl-diamine chelating unit and pendant l-arginine analogues (substrates and inhibitors of NOS). Reaction of the conjugates with fac-[M(CO)(3)](+) (M = Re, (99m)Tc) gave bioorganometallic complexes of the type fac-[M(CO)(3)(k(3)-L)] in good yield. After in vitro testing using the oxyhemoglobin NO capture assay, we concluded that the affinity of the inhibitor-containing conjugates to iNOS seems to be less affected upon metallation with rhenium than the substrate-containing conjugates. The complexes bearing guanidino substituted analogues of l-arginine still present considerable inhibitory action (N(omega)-monomethyl-l-arginine, K(i) = 36 microM; N(omega)-nitro-l-arginine, K(i) = 84 microM), being the first examples of organometallic complexes able to inhibit the iNOS. These results seem to indicate that (99m)Tc(CO)(3)-labeled L-argininine analogues, namely NOS inhibitors, may hold potential for monitoring increased levels of iNOS in vivo.
Oliveira
Bruno L
BL
Departamento de Química, ITN, Estrada Nacional 10, 2686-953, Sacavém, Portugal.
Correia
João D G
JD
Raposinho
Paula D
PD
Santos
Isabel
I
Ferreira
António
A
Cordeiro
Carlos
C
Freire
Ana P
AP
D001120:Arginine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015335:Molecular Probes; D052247:Nitric Oxide Synthase Type II / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D015609:Organotechnetium Compounds / Q000737:chemistry*; D012211:Rhenium / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D015335:Molecular Probes; D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; D012211:Rhenium; D001120:Arginine; D052247:Nitric Oxide Synthase Type II