title: Highly sensitive method for determination of N-nitrosamines using high-performance liquid chromatography with online UV irradiation and luminol chemiluminescence detection.
abstract: A new method for the measurement of N-nitrosamines in part-per-trillion concentrations from water samples without preconcentration steps has been developed. This method is based on online UV irradiation after high-performance liquid chromatographic separation and subsequent luminol chemiluminescence detection without addition of an oxidant. It was confirmed that N-nitrosamines in basic aqueous solution were transformed to peroxynitrite by UV irradiation. The detection limits for this method were 1.5 ng/L, 2.9 ng/L, 3.0 ng/L, and 2.7 ng/L for N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine, respectively, at a signal-to-noise ratio of 3. The calibration graphs were linear in the range of 5-1000 ng/L for these N-nitrosamines. This method was used for the determination of N-nitrosamines in tap water, river water, and industrial plant effluent samples. The recoveries of N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine present in tap water sample at a concentration of 10 ng/L (mean+/-standard deviation, n=4) were (94.8+/-2.7)%, (102.0+/-6.9)%, (99.3+/-3.9)%, and (102.8+/-2.5)%, respectively. These results indicate that our proposed method can be applied satisfactorily to the determination of N-nitrosamines in water samples.
authors.affiliation: Faculty of Science, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, Japan. kodama@sci.kagoshima-u.ac.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kodamatani
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Keiitsu
authors.initials: K
authors.lastname: Saito
authors.forename: Abena
authors.initials: A
authors.lastname: Amponsaa-Karikari
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Takashi
authors.initials: T
authors.lastname: Tomiyasu
authors.forename: Yu
authors.initials: Y
authors.lastname: Komatsu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004128:Dimethylnitrosamine; D008163:Luminescent Measurements; D008165:Luminol / Q000032:analysis* / Q000737:chemistry; D009242:N-Nitrosopyrrolidine; D009602:Nitrosamines / Q000032:analysis*; D030421:Peroxynitrous Acid / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D009602:Nitrosamines; D014874:Water Pollutants, Chemical; D030421:Peroxynitrous Acid; C002741:N-nitrosomorpholine; D008165:Luminol; D004128:Dimethylnitrosamine; D009242:N-Nitrosopyrrolidine