title: Odontoblasts in the dental pulp immune response.
abstract: Recent studies have demonstrated that human dental pulp cells sense pathogens and elicit innate and/or adaptive immunity. Particular attention has been paid to odontoblasts that are situated at the pulp-dentin interface and constitute the first line of defense to cariogenic bacteria entering dentin after enamel disruption. In this review, recent in vitro and in vivo data suggesting that odontoblasts initiate immune/inflammatory events within the dental pulp in response to cariogenic bacteria are discussed. These data include sensing of pathogens by Toll-like receptors (TLRs), production of chemokines upon cell stimulation with microbial by-products and induction of dendritic cell migration. Additional results presented here reveal that all TLR genes are expressed in the healthy human dental pulp that is thus well equipped to combat pathogens entering the tissue. Seventeen chemokine genes including CXCL12, CCL2, CXCL9, CX3CL1, CCL8, CXCL10, CCL16, CCL5, CXCL2, CCL4, CXCL11 and CCL3, and 9 chemokine receptor genes including CXCR4, CCR1, CCR5, CX3CR1, CCR10 and CXCR3, are also expressed in pulp. TLR2, CCL2 and CXCL1 are upregulated in odontoblasts both under caries lesions and upon stimulation with pathogen by-products. These molecules thus appear as preferential targets for the design of therapeutic agents able to reduce the immune/inflammatory response to cariogenic bacteria and favor pulp healing.
authors.affiliation: Université de Lyon, Université Lyon 1, Institut de Génomique Fonctionnelle de Lyon, CNRS, INRA, Ecole Normale Supérieure de Lyon, Lyon, France. Jean-Christophe.Farges@sante.univ-lyon1.fr
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Farges
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Keller
authors.forename: Florence
authors.initials: F
authors.lastname: Carrouel
authors.forename: Stephanie H
authors.initials: SH
authors.lastname: Durand
authors.forename: Annick
authors.initials: A
authors.lastname: Romeas
authors.forename: Françoise
authors.initials: F
authors.lastname: Bleicher
authors.forename: Serge
authors.initials: S
authors.lastname: Lebecque
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Staquet
mesh_terms: D001424:Bacterial Infections / Q000276:immunology; D018925:Chemokines / Q000235:genetics / Q000502:physiology; D017124:Conserved Sequence; D003731:Dental Caries / Q000276:immunology / Q000473:pathology; D003782:Dental Pulp / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008963:Molar / Q000276:immunology / Q000473:pathology; D009804:Odontoblasts / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D019707:Receptors, Chemokine / Q000235:genetics; D013682:Teichoic Acids / Q000494:pharmacology; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology; D014076:Tooth Diseases / Q000276:immunology
chemical_list: D018925:Chemokines; D008070:Lipopolysaccharides; D019707:Receptors, Chemokine; D013682:Teichoic Acids; D051193:Toll-Like Receptors; C009900:lipoteichoic acid