Template usage is responsible for the preferential acquisition of the K65R reverse transcriptase mutation in subtype C variants of human immunodeficiency virus type 1.
We propose that a nucleotide template-based mechanism facilitates the acquisition of the K65R mutation in subtype C human immunodeficiency virus type 1 (HIV-1). Different patterns of DNA synthesis were observed using DNA templates from viruses of subtype B or C origin. When subtype C reverse transcriptase (RT) was employed to synthesize DNA from subtype C DNA templates, preferential pausing was seen at the nucleotide position responsible for the AAG-to-AGG K65R mutation. This did not occur when the subtype B RT and template were used. Template factors can therefore increase the probability of K65R development in subtype C HIV-1.
Coutsinos
Dimitrios
D
McGill University AIDS Center, McGill University, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Canada.
Invernizzi
Cédric F
CF
Xu
Hongtao
H
Moisi
Daniela
D
Oliveira
Maureen
M
Brenner
Bluma G
BG
Wainberg
Mark A
MA
D019943:Amino Acid Substitution / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D054303:HIV Reverse Transcriptase / Q000235:genetics* / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D020125:Mutation, Missense; D013698:Templates, Genetic
D004279:DNA, Viral; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase