title: Haplotype diversity in human mitochondrial DNA hypervariable regions I-III in the city of Caracas (Venezuela).
abstract: In order to expand the database of variable DNA for forensic identification purposes in Venezuela, we analyzed the sequence polymorphisms of mitochondrial DNA (mtDNA) hypervariable regions (HVR) I-III from 100 unrelated individuals from the city of Caracas, using PCR amplification and fluorescent-based capillary electrophoresis sequencing method. Dominant haplogroups corresponded to Native Americans followed by African ones. The inclusion of HVR III although useful for sub-haplogroup assignation, added little to the discrimination capacity of our database.
authors.affiliation: Centro de Biotecnología, Fundación Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela.
authors.forename: N
authors.initials: N
authors.lastname: Lander
authors.forename: M G
authors.initials: MG
authors.lastname: Rojas
authors.forename: M A
authors.initials: MA
authors.lastname: Chiurillo
authors.forename: J L
authors.initials: JL
authors.lastname: Ramírez
mesh_terms: D044383:Black People / Q000235:genetics; D022801:Complementarity Determining Regions / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D053803:Forensic Genetics / Q000379:methods; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D016133:Polymerase Chain Reaction; D014505:Urban Population; D014687:Venezuela
chemical_list: D022801:Complementarity Determining Regions; D017931:DNA Primers; D004272:DNA, Mitochondrial