Basic phage electron microscopy.
Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.
Ackermann
Hans-W
HW
Department of Medical Microbiology, Faculty of Medicine, Laval University, Quebec, QC, Canada.
D001435:Bacteriophages / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods*; D009942:Organometallic Compounds; D010772:Phosphotungstic Acid
D009942:Organometallic Compounds; D010772:Phosphotungstic Acid; C005460:uranyl acetate