MiniY-STR quadruplex systems with short amplicon lengths for analysis of degraded DNA samples.
Two short amplicon Y-chromosomal short tandem repeat (miniY-STR) quadruplex systems for the eight Y-STR loci DYS522, DYS508, DYS632, DYS556, DYS570, DYS576, DYS504 and DYS540 were devised using newly designed primer sets. Among 224 samples from Japanese population, amplification product lengths detected in these Y-STR loci ranged from 95 to 147bp, while 170 different haplotype were identified (discrimination capacity=0.7589 and haplotype diversity=0.9949). As a result of test on degraded DNA samples using the miniY-STR quadruplex systems, the systems proved to be an quite effective tools for analyzing degraded DNAs. We conclude that analyses of the miniY-STR quadruplex systems in addition to the commercial available Y-STR multiplex kits are highly useful for forensic practices of degraded DNA samples.
Asamura
H
H
Department of Legal Medicine, Shinshu University School of Medicine, Japan.
Sakai
H
H
Ota
M
M
Fukushima
H
H
D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods / Q000706:statistics & numerical data; D017931:DNA Primers / Q000235:genetics; D005260:Female; D053803:Forensic Genetics / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004247:DNA