Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system.
Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.
Ko
Kwang-Wook
KW
Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
Lin
Fengqiu
F
Katsumata
Takumi
T
Sugai
Yoshinori
Y
Miyazaki
Sho
S
Kawaide
Hiroshi
H
Okada
Kazunori
K
Nojiri
Hideaki
H
Yamane
Hisakazu
H
D000595:Amino Acid Sequence; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression; D008861:Microsomes / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D012275:Oryza / Q000201:enzymology*; D010843:Pichia / Q000166:cytology / Q000235:genetics*
D003577:Cytochrome P-450 Enzyme System; C066398:ent-kaurene oxidase; D009251:NADPH-Ferrihemoprotein Reductase