Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP).
THOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50-137, amino acid numbers) binds to the N-terminal portion (1-199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.
El Bounkari
Omar
O
Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Hannover, Germany.
Guria
Anuja
A
Klebba-Faerber
Sabine
S
Claussen
Maike
M
Pieler
Tomas
T
Griffiths
John R
JR
Whetton
Anthony D
AD
Koch
Alexandra
A
Tamura
Teruko
T
D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009154:Mutation; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D012322:RNA Precursors / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism
D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; C530378:THOC5 protein, human; C538994:THOC7 protein, human; D029867:Xenopus Proteins