[Curcumin inhibits growth, induces G1 arrest and apoptosis on human prostatic stromal cells by regulating Bcl-2/Bax].
OBJECTIVE
To investigate the effect of curcumin on apoptosis of human prostatic stromal cells.


METHODS
Different concentrations of curcumin were added into culture media system to induce apoptosis of human prostatic stromal cells. The apoptosis was detected by MTT assay, flow cytometry, RT-PCR and TUNEL method.


RESULTS
Curcumin at concentrations of 10-40 micromol x L(-1) could inhibit the proliferation of human prostatic stromal cells in a doseand time-dependent manner (P < 0.05). Characteristic apoptosis were confirmed by TUNEL RT-PCR manifest downregulation of Bcl-2/Bax (P < 0.05). Cell cycle was arrested into G1 phase by curcumin.


CONCLUSIONS
Curcumin can induce apoptosis in human prostatic stromal cells by down-regulation of Bcl-2/Bax.
Chen
Zhi-qiang
ZQ
Institute of Urology & Nephrology, First Affiliated Hospital, Guangxi Medical Universuity, Nanning 530021, China.
Jie
Xiao
X
Mo
Zeng-nan
ZN
D017209:Apoptosis / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D011467:Prostate / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D051028:bcl-2-Associated X Protein; D003474:Curcumin