Nanoscale separation of molecular species based on their rotational mobility.
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.
Testa
Ilaria
I
Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Schönle
Andreas
A
von Middendorff
Claas
C
Geisler
Claudia
C
Medda
Rebecca
R
Wurm
Christian A
CA
Stiel
Andre C
AC
Jakobs
Stefan
S
Bossi
Mariano
M
Eggeling
Christian
C
Hell
Stefan W
SW
Egner
Alexander
A
D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D012399:Rotation
D001704:Biopolymers