Biodegradation of 1,4-dioxane and transformation of related cyclic compounds by a newly isolated Mycobacterium sp. PH-06.
A new bacterial strain PH-06 was isolated using enrichment culture technique from river sediment contaminated with 1,4-dioxane, and identified as belonging to the genus Mycobacterium based on 16S rRNA sequencing (Accession No. EU239889). The isolated strain effectively utilized 1,4-dioxane as a sole carbon and energy source and was able to degrade 900 mg/l 1,4-dioxane in minimal salts medium within 15 days. The key degradation products identified were 1,4-dioxane-2-ol and ethylene glycol, produced by monooxygenation. Degradation of 1,4-dioxane and concomitant formation of metabolites were demonstrated by GC/MS analysis using deuterium labeled 1,4-dioxane (1,4-dioxane-d8). In addition to 1,4-dioxane, this bacterium could also transform structural analogues such as 1,3-dioxane, cyclohexane and tetrahydrofuran when pre-grown with 1,4-dioxane as the sole growth substrate. Our results suggest that PH-06 can maintain sustained growth on 1,4-dioxane without any other carbon sources.
Kim
Young-Mo
YM
School of Environmental Science and Engineering, POSTECH, Pohang, South Korea.
Jeon
Jong-Rok
JR
Murugesan
Kumarasamy
K
Kim
Eun-Ju
EJ
Chang
Yoon-Seok
YS
D004146:Dioxanes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004146:Dioxanes; D012336:RNA, Ribosomal, 16S; C025223:1,4-dioxane