title: Diagnosis of pancreatic adenocarcinoma using protein chip technology.
abstract: BACKGROUND
  To develop a serum-specific protein fingerprint which is capable of differentiating samples from patients with pancreatic cancer and those with other pancreatic conditions.
  
  
  METHODS
  We used SELDI-TOF-MS coupled with CM10 chips and bioinformatics tools to analyze a total of 118 serum samples in this study; 78 serum samples were analyzed to establish the diagnostic models and the other 40 samples were analyzed on the second day as an independent test set.
  
  
  RESULTS
  The analysis of this independent test set yielded a specificity of 91.6% and a sensitivity of 91.6% for pattern 1, which distinguished pancreatic adenocarcinoma (PC) from healthy individuals and a specificity of 80.0% and a sensitivity of 90.9% for pattern 2, which distinguished PC from chronic pancreatitis.
  
  
  CONCLUSIONS
  This study indicated that the SELDI-TOF-MS technique can facilitate the discovery of better serum tumor biomarkers and a combination of specific models is more accurate than a single model in diagnosis of PC.
authors.affiliation: Department of Surgery, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China.
authors.forename: Daren
authors.initials: D
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Cao
authors.forename: Jiekai
authors.initials: J
authors.lastname: Yu
authors.forename: Risheng
authors.initials: R
authors.lastname: Que
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Jiang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhou
authors.forename: Linhua
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D014408:Biomarkers, Tumor / Q000097:blood*; D018395:CA-19-9 Antigen / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis / Q000175:diagnosis; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D050500:Pancreatitis, Chronic / Q000175:diagnosis; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen