title: PCR based RFLP genotyping of bovine lymphocyte antigen DRB3.2 in Iranian Holstein population.
abstract: Major Histocompatibility Complex (MHC) class II locus DRB3 was investigated by PCR based restriction fragment length polymorphism (PCR-RFLP) assay. A total of 262 Holstein cows participating in the national recording system were sampled from 10 herds. A two-step polymerase chain reaction was carried out in order to amplify a 284 base-pair fragment of exon 2 of the target gene. Second PCR products were treated with three restriction endonucleas enzymes RsaI, BstYI and HaeIII. Digested fragments were analyzed by polyacrylamid gel electrophoresis. Twenty-eight BoLA-DRB3 alleles were identified. Identified alleles are: BoLA-DRB3.2 *3, *6, *7, *8, *9, *10, *11, *12, *13, *14, *15, *16,20, *21, *22, *23, *24, *25, *26, *27, *28, *32, *36, *37, *40, *51, *iaa and *ibb. The BoLA-DRB3.2*40 allele that was observed in this study has not been reported previously. The calculated frequencies were as follows: 2.29, 1.34, 0.19, 14.5, 0.38, 3.05, 12.21, 1.34, 2.29, 1.34, 2.48, 9.16, 0.95, 0.77, 6.68, 9.16, 17.94, 1.15, 0.57, 1.15, 0.95, 0.57, 0.38, 1.91, 0.38, 5.73, 0.19 and 0.95% respectively. The six most frequently observed alleles (BoLA-DRB3.2 *8, *11, *16, *22, *23 and *24) accounted for 69.65% of the alleles in these 10 herds. The results of this study confirm the allelic distribution of six most frequent alleles in Holstein population's worldwide.
authors.affiliation: Animal Genetics Division, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Pashmi
authors.forename: Saber
authors.initials: S
authors.lastname: Qanbari
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Ghorashi
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Salehi
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059826:HLA-DRB3 Chains; D007492:Iran; D008214:Lymphocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*
chemical_list: D006684:HLA-DR Antigens; D059826:HLA-DRB3 Chains