title: Long-term garlic or micronutrient supplementation, but not anti-Helicobacter pylori therapy, increases serum folate or glutathione without affecting serum vitamin B-12 or homocysteine in a rural Chinese population.
abstract: The effects of a 7.3-y supplementation with garlic and micronutrients and of anti-Helicobacter pylori treatment with amoxicillin (1 g twice daily) and omeprazole (20 mg twice daily) on serum folate, vitamin B-12, homocysteine, and glutathione concentrations were assessed in a rural Chinese population. A randomized, double-blind, placebo-controlled, factorial trial was conducted to compare the ability of 3 treatments to retard the development of precancerous gastric lesions in 3411 subjects. The treatments were: 1) anti-H. pylori treatment with amoxicillin and omeprazole; 2) 7.3-y supplementation with aged garlic and steam-distilled garlic oil; and 3) 7.3-y supplementation with vitamin C, vitamin E, and selenium. All 3 treatments were given in a 2(3) factorial design to subjects seropositive for H. pylori infection; only the garlic supplement and vitamin and selenium supplement were given in a 2(2) factorial design to the other subjects. Thirty-four subjects were randomly selected from each of the 12 treatment strata. Sera were analyzed after 7.3 y to measure effects on folate, vitamin B-12, homocysteine, and glutathione concentrations. Regression analyses adjusted for age, gender, and smoking indicated an increase of 10.2% (95%CI: 2.9-18.1%) in serum folate after garlic supplementation and an increase of 13.4% (95%CI: 5.3-22.2%) in serum glutathione after vitamin and selenium supplementation. The vitamin and selenium supplement did not affect other analytes and the amoxicillin and omeprazole therapy did not affect any of the variables tested. In this rural Chinese population, 7.3 y of garlic supplementation increased the serum folate concentration and the vitamin and selenium supplement increased that of glutathione, but neither affected serum concentrations of vitamin B-12 or homocysteine.
authors.affiliation: Key Laboratory of Carcinogenesis and Translation Research (Ministry of Education), Department of Epidemiology, Peking University School of Oncology, Beijing Cancer Hospital and Institute, Hai-dian District, Beijing 100036, PR China.
authors.forename: Yujue
authors.initials: Y
authors.lastname: Wang
authors.forename: Lian
authors.initials: L
authors.lastname: Zhang
authors.forename: Roxana
authors.initials: R
authors.lastname: Moslehi
authors.forename: Junling
authors.initials: J
authors.lastname: Ma
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Pan
authors.forename: Tong
authors.initials: T
authors.lastname: Zhou
authors.forename: Weidong
authors.initials: W
authors.lastname: Liu
authors.forename: Linda Morris
authors.initials: LM
authors.lastname: Brown
authors.forename: Yuangreng
authors.initials: Y
authors.lastname: Hu
authors.forename: David
authors.initials: D
authors.lastname: Pee
authors.forename: Mitchell H
authors.initials: MH
authors.lastname: Gail
authors.forename: Weicheng
authors.initials: W
authors.lastname: You
mesh_terms: D000328:Adult; D000658:Amoxicillin / Q000008:administration & dosage / Q000494:pharmacology*; D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D044466:Asian People; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005260:Female; D005492:Folic Acid / Q000097:blood; D005737:Garlic; D005978:Glutathione / Q000097:blood; D016481:Helicobacter Infections / Q000517:prevention & control*; D016480:Helicobacter pylori; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008297:Male; D018977:Micronutrients / Q000494:pharmacology*; D008875:Middle Aged; D009853:Omeprazole / Q000008:administration & dosage / Q000494:pharmacology*; D012424:Rural Population; D014805:Vitamin B 12 / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D018977:Micronutrients; D006710:Homocysteine; D000658:Amoxicillin; D005492:Folic Acid; D005978:Glutathione; D009853:Omeprazole; D014805:Vitamin B 12