A functional putative phytochelatin synthase from the primitive red alga Cyanidioschyzon merolae.
Phytochelatin synthase (PCS) catalyzes the synthesis of phytochelatins (PCs), which play a detoxification role in higher plants. Heterologous expression of CmPCS, a product of a PCS-like gene from the genomic DNA of the red alga Cyanidioschyzon merolae, rescued Cd(2+)-sensitive yeast from Cd(2+) toxicity. The fact that these transformed cells synthesized PCs demonstrates that CmPCS is functional.
Osaki
Yuko
Y
Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Shirabe
Tomoo
T
Tamura
Shun
S
Yoshimura
Etsuro
E
D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000737:chemistry / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity; D015870:Gene Expression; D008969:Molecular Sequence Data; D054811:Phytochelatins / Q000096:biosynthesis; D000461:Rhodophyta / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D002104:Cadmium; D054811:Phytochelatins; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase