Evaluation of an injectable, photopolymerizable, and three-dimensional scaffold based on methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) combined with autologous mesenchymal stem cells in a goat tibial unicortical defect model.
An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and alpha-tricalcium phosphate (alpha-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of alpha-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.
Vertenten
Geert
G
Department of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. geert.vertenten@ugent.be
Lippens
Evi
E
Gironès
Jordi
J
Gorski
Tomasz
T
Declercq
Heidi
H
Saunders
Jimmy
J
Van den Broeck
Wim
W
Chiers
Koen
K
Duchateau
Luc
L
Schacht
Etiene
E
Cornelissen
Maria
M
Gasthuys
Frank
F
Vlaminck
Lieven
L
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D002469:Cell Separation; D002478:Cells, Cultured; D006041:Goats; D007150:Immunohistochemistry; D007267:Injections; D008027:Light; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D008954:Models, Biological; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology* / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry*
D008689:Methacrylates; D011091:Polyesters; C062968:lactide-caprolactone copolymer