New alleles and mutational events at 14 STR loci from different German populations.
The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.
Becker
Dorit
D
Biotype AG, Moritzburger Weg 67 D, 01109 Dresden, Germany. d.becker@biotype.de
Bender
Klaus
K
Edelmann
Jeanett
J
Götz
Frank
F
Henke
Lotte
L
Hering
Sandra
S
Hohoff
Carsten
C
Hoppe
Karolin
K
Klintschar
Michael
M
Muche
Matthias
M
Rolf
Burkhard
B
Szibor
Reinhard
R
Weirich
Volker
V
Jung
Martin
M
Brabetz
Werner
W
D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D053803:Forensic Genetics; D005787:Gene Frequency; D005828:Genetics, Population; D005858:Germany; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009154:Mutation
D017931:DNA Primers; D004247:DNA