Comparison of telogen hair analyses: genRES MPX-2SP kit versus genRES MPX-SP1 and genRES MPX-SP2 kits.
STR investigations of telogen hair are invariably difficult due to the small amounts of nuclear DNA and its degradation products. However, in recent years there has been a considerable improvement. This study examined the suitability of a new STR kit with shortened amplicons for the investigation of hair in routine casework. This kit allows the simultaneous amplification of the eight STR-loci D3S1358, VWA, FGA, TH01, SE33, D8S1179, D18S51, and D21S11, and the sex-determining amelogenin system. It was tested against the genRES MPX-SP1 and genRES MPX-SP2 kits. The sensitivity of the new genRES MPX-2SP kit was demonstrated to be inferior to that of the genRES MPX-SP1, but almost equal to that of the genRES MPX-SP2 kit.
Schmid
D
D
Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Nussbaumstr 26, 80335 Munich, Germany. dagmar.schmid@med.uni-muenchen.de
Bayer
B
B
Anslinger
K
K
D053523:Amelogenin / Q000235:genetics*; D002467:Cell Nucleus / Q000737:chemistry; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D030541:Databases, Genetic; D005260:Female; D005784:Gene Amplification; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D019849:Sex Determination Processes
D053523:Amelogenin; D004272:DNA, Mitochondrial; D011933:Reagent Kits, Diagnostic; D004247:DNA