Chemiluminescence detector with a serpentine flow cell.
We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available "BacTiter-Glo" reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell.
Terry
Jessica M
JM
School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.
Adcock
Jacqui L
JL
Olson
Don C
DC
Wolcott
Duane K
DK
Schwanger
Cassie
C
Hill
Lauren A
LA
Barnett
Neil W
NW
Francis
Paul S
PS