title: Protein expression profiling during chick retinal maturation: a proteomics-based approach.
abstract: BACKGROUND
  The underlying pathways that drive retinal neurogenesis and synaptogenesis are still relatively poorly understood. Protein expression analysis can provide direct insight into these complex developmental processes. The aim of this study was therefore to employ proteomic analysis to study the developing chick retina throughout embryonic (E) development commencing at day 12 through 13, 17, 19 and post-hatch (P) 1 and 33 days.
  
  
  RESULTS
  2D proteomic and mass spectrometric analysis detected an average of 1514 spots per gel with 15 spots demonstrating either modulation or constitutive expression identified via MS. Proteins identified included alpha and beta-tubulin, alpha enolase, B-creatine kinase, gamma-actin, platelet-activating factor (PAF), PREDICTED: similar to TGF-beta interacting protein 1, capping protein (actin filament muscle Z line), nucleophosmin 1 (NPM1), dimethylarginine dimethylaminohydrolase, triosphoaphate isomerase, DJ1, stathmin, fatty acid binding protein 7 (FABP7/B-FABP), beta-synuclein and enhancer of rudimentary homologue.
  
  
  CONCLUSIONS
  This study builds upon previous proteomic investigations of retinal development and represents the addition of a unique data set to those previously reported. Based on reported bioactivity some of the identified proteins are most likely to be important to normal retinal development in the chick. Continued analysis of the dynamic protein populations present at the early stages and throughout retinal development will increase our understanding of the molecular events underpinning retinogenesis.
authors.affiliation: Centre for Vision Sciences, Queen's University of Belfast, Institute of Clinical Sciences, Royal Victoria Hospital, Belfast, Northern Ireland. s.finnegan@ucc.ie
authors.forename: Sorcha
authors.initials: S
authors.lastname: Finnegan
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Robson
authors.forename: Mildred
authors.initials: M
authors.lastname: Wylie
authors.forename: Adrienne
authors.initials: A
authors.lastname: Healy
authors.forename: Alan W
authors.initials: AW
authors.lastname: Stitt
authors.forename: William J
authors.initials: WJ
authors.lastname: Curry