Whole-cell recordings of light evoked excitatory synaptic currents in the retinal slice.
We use the whole-cell patch clamp technique to study the synaptic circuitry that underlies visual information processing in the retina. In this video, we will guide you through the process of performing whole-cell recordings of light evoked currents of individual cells in the retinal slice preparation. We use the aquatic tiger salamander as an animal model. We begin by describing the dissection of the eye and show how slices are mounted for electrophysiological recordings. Once the slice is placed in the recording chamber, we demonstrate how to perform whole-cell voltage clamp recordings. We then project visual stimuli onto the photoreceptors in the slice to elicit light-evoked current responses. During the recording we perfuse the slice with pharmacological agents, whereby an 8-channel perfusion system allows us to quickly switch between different agents. The retinal slice preparation is widely used for patch clamp recordings in the retina, in particular to study amacrine or bipolar cells, which are not accessible in a whole-mount preparation.
Werner
Birgit
B
Program in Neuroscience, Boston University, USA. birgit@bu.edu
Cook
Paul B
PB
Passaglia
Christopher L
CL
D000818:Animals; D004210:Dissection / Q000379:methods; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D005123:Eye / Q000033:anatomy & histology; D008027:Light; D008867:Microtomy / Q000379:methods; D018408:Patch-Clamp Techniques / Q000379:methods*; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology; D012160:Retina / Q000502:physiology*; D013569:Synapses / Q000502:physiology*