A rapid and sensitive immunonanogold resonance scattering spectral probe for complement 3.
Gold nanoparticles in size of about 10nm was used to label goat anti-human complement 3 (anti-C3) to obtain a sensitive and selective immunoresonance scattering spectral probe for C3. It was based on the immune reaction between labeled anti-C3 and C3 in the pH 5.6 Na(2)HPO(4)-citric acid buffer solutions and in presence of polyethylene glycol (PEG). The resonance scattering (RS) intensity at 560nm enhanced greatly with C3. Well linear relationships between the enhanced RS intensity ( big up tri, openI(RS)) and the C3 concentration in the range of 8.33-200ngml(-1) were obtained, with a detection limit of 2.8ngml(-1) and the limit of quantification 8.51ngml(-1) for C3. The convenient and selective and sensitive assay was applied to quantification of C3 in human sera, with satisfactory results.
Jiang
Zhiliang
Z
School of Environment and Resource, Guangxi Normal University, Guilin 541004, China; Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China.
Huang
Wenxin
W
Liang
Aihui
A
Chen
Bing
B