title: Combination of different molecular mechanisms leading to fluconazole resistance in a Candida lusitaniae clinical isolate.
abstract: We report on the underlying molecular mechanisms likely responsible for the high-level fluconazole resistance in a Candida lusitaniae clinical isolate. Fluconazole resistance correlated with overexpression of ERG11 and of several efflux pump genes, in particular, the orthologs of the Candida albicans MDR1, PDR16, CDR1, CDR2, and YOR1.
authors.affiliation: Programme Chimiorésistance des Levures Pathogènes, EA209, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 75006 Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Reboutier
authors.forename: Mathieu
authors.initials: M
authors.lastname: Piednoël
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boisnard
authors.forename: Audrey
authors.initials: A
authors.lastname: Conti
authors.forename: Virginie
authors.initials: V
authors.lastname: Chevalier
authors.forename: Martine
authors.initials: M
authors.lastname: Florent
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Gibot-Leclerc
authors.forename: Bruno
authors.initials: B
authors.lastname: Da Silva
authors.forename: Christiane
authors.initials: C
authors.lastname: Chastin
authors.forename: Karim
authors.initials: K
authors.lastname: Fallague
authors.forename: Anne
authors.initials: A
authors.lastname: Favel
authors.forename: Thierry
authors.initials: T
authors.lastname: Noël
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Ruprich-Robert
authors.forename: Florence
authors.initials: F
authors.lastname: Chapeland-Leclerc
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D002175:Candida / Q000187:drug effects* / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D017931:DNA Primers; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D015725:Fluconazole; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D008780:Methyltransferases