title: The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice.
abstract: Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Andrey P
authors.initials: AP
authors.lastname: Anisimov
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Bakhteeva
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Evgeniy A
authors.initials: EA
authors.lastname: Panfertsev
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Tat'yana E
authors.initials: TE
authors.lastname: Svetoch
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Tat'yana B
authors.initials: TB
authors.lastname: Kravchenko
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Mikhail E
authors.initials: ME
authors.lastname: Platonov
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Galina M
authors.initials: GM
authors.lastname: Titareva
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Tat'yana I
authors.initials: TI
authors.lastname: Kombarova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Ivanov
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkofer Str. 9a, 80336 Munich, Germany.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Rakin
authors.affiliation: Canadian Food Inspection Agency Lethbridge Laboratory, PO 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada.
authors.forename: Kingsley K
authors.initials: KK
authors.lastname: Amoako
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Dentovskaya
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D005260:Female; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D014774:Virulence; D015009:Yersinia Infections / Q000276:immunology* / Q000382:microbiology; D015010:Yersinia pestis / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C081814:pH 6 antigen, Yersinia