Determination of the alkyl- and methoxy-phenolic content in wood extractives by micellar solid-phase microextraction and gas chromatography-mass spectrometry.
This work combines the utilization of the micellar media during the extraction step and the focused microwave-assisted extraction followed by the solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) to determine the alkyl- and methoxy-phenolic content in wood extractives. The proposed environmental-friendly method is mainly characterized by short analysis times (5min for the microwaves extraction step) and for avoiding the use of organic solvents. Different surfactants were tried for the extraction process: the cationic surfactant CTAB and the non-ionic surfactants Triton X-100 and POLE, with similar extraction efficiencies (85.5-99.7%). The overall method presents limits of detection in the ngg(-1) region for the alkylphenols (from 7 to 150ngg(-1)) and in the mugg(-1) region for the methoxyphenols (from 0.80 to 22.9mugg(-1)). The vanillin was the compound most abundant in the wood extractives studied, with concentrations up to 116.2mgkg(-1).
Pino
Verónica
V
Department of Analytical Chemistry, Nutrition and Food Science, University of La Laguna. Campus de Anchieta, Astrofísico Francisco Sánchez s/n, E-38205 La Laguna, Spain.
Ayala
Juan H
JH
González
Venerando
V
Afonso
Ana M
AM