[The role of growth factors in inhibition of collagen degeneration and chondrocyte differentiation in patients with osteoarthrosis].
Degeneration of collagen in 23 explants of articular cartilage from patients with osteoarthrosis (OA) was suppressed by a combination of growth factors that were used in the past to inhibit hypertrophy of oral plate chondrocytes, viz. transforming growth factor -beta 2 (TGFb2), fibroblast growth factor-2 (FGF-2), and insulin. Their integrated inhibitory activity amounted to 56%, with TGFb being the most efficient of them (59%). Inhibition of enhanced collagen degeneration by these growth factors was associated with reduced expression of the genes controlling chondrocyte hypertrophy, type 10 collagen (COL10A1), collagenase-3 (MMP-13), matrix-9 metalloproteinase (MMP-9), cytokines IL-la/b and TNFa, prostaglandin E synthase (PGES-1), and release of prostaglandin E2 (PGE2) into the culture medium. On the other hand, these growth factors alone or in different combinations did not inhibit degradation of proteoglycans (such as aggrecan), with the exception of TGFb2 that in certain cases stimulated degradation. To conclude, growth factors TGFb2, FGF-2, and insulin suppress in vitro collagen degradation and chondrocyte differentiation in cartilaginous tissue of patients with OA, possibly with the participation of PGE2. They can be used to prevent collagen-2 degeneration in these patients.
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D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics
D036341:Intercellular Signaling Peptides and Proteins; D012313:RNA; D003094:Collagen