ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.
Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, and may be readily elaborated to many different assay formats.
Leipold
Michael D
MD
Department of Chemistry, University of Toronto, Toronto, Ontario, M5S 3H6, Canada.
Herrera
Isaac
I
Ornatsky
Olga
O
Baranov
Vladimir
V
Nitz
Mark
M
D000818:Animals; D002614:Chelating Agents / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*
D002614:Chelating Agents; D006023:Glycoproteins; D028581:Lanthanoid Series Elements; D037102:Lectins; D011108:Polymers