An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II.
Genetic evidence indicates that plant-specific homologs of DNA-dependent RNA polymerase (Pol) II large subunits form Pol IV and Pol V complexes involved in small interfering RNA production and RNA-directed DNA methylation. Here we describe evidence that Pol V contains subunits shared with Pol II, but that RNA polymerase II subunit (RPB)-4 is missing from Pol V and that RPB5 is present as a Pol V-specific isomer, RPB5b. Pol V also has other proteins that are not present in Pol II, consistent with a role of this complex as an effector of silencing.
Huang
Linfeng
L
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Jones
Alexandra M E
AM
Searle
Iain
I
Patel
Kanu
K
Vogler
Hannes
H
Hubner
Nina C
NC
Baulcombe
David C
DC
D004247:DNA / Q000235:genetics / Q000378:metabolism; D019951:DNA Polymerase beta / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D034622:RNA Interference; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D021122:Protein Subunits; D034741:RNA, Small Interfering; D004247:DNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D019951:DNA Polymerase beta