Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells.
BRMS1 and SUDS3 are related members of SIN3-HDAC chromatin remodeling complexes. We hypothesized that they might have overlapping functions and that SUDS3 over-expression could compensate for BRMS1 deficiency. SUDS3 expression was ubiquitous in seven breast cell lines, regardless of metastatic potential. SUDS3 over-expression in BRMS1-non-expressing metastatic cells did not suppress metastasis, motility, osteopontin secretion, or EGF receptor expression, phenotypes associated with BRMS1-mediated metastasis suppression. This study demonstrates functional differences for BRMS1 family members and highlights how the composition of SIN3-HDAC (BRMS1/SUDS3) complexes uniquely affects protein expression and biological behaviors.
Silveira
Alexandra C
AC
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35209, USA.
Hurst
Douglas R
DR
Vaidya
Kedar S
KS
Ayer
Donald E
DE
Welch
Danny R
DR
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D056264:Sin3 Histone Deacetylase and Corepressor Complex; D014162:Transfection
C410024:BRMS1 protein, human; D009363:Neoplasm Proteins; D012097:Repressor Proteins; D006655:Histone Deacetylases; D056264:Sin3 Histone Deacetylase and Corepressor Complex