Novel synthetic collagen fibers, poly(PHG), stimulate platelet aggregation through glycoprotein VI.
Novel synthetic collagen fibers, poly(PHG) made by polycondensation of Pro-Hyp-Gly, spontaneously assume polymeric structure with molecular weights greater than 10(5). Its application for biomaterials has been explored, but that for a platelet agonist has not been investigated. Poly(PHG)-induced platelet aggregation independently of thromboxane A(2) and integrin alpha2beta1. Poly(PHG)-induced tyrosine phosphorylation of glycoprotein VI (GPVI)-related molecules and failed to activate GPVI/FcRgamma-deficient platelets. Binding of GPVI to poly(PHG) was confirmed by a surface plasmon resonance spectroscopy, suggesting that poly(PHG) activates platelets through GPVI. Poly(PHG) is an useful research tool to investigate GPVI-mediated signals and a substitute for collagen in platelet functional assays.
Inoue
Osamu
O
Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan.
Suzuki-Inoue
Katsue
K
Shinoda
Daisuke
D
Umeda
Yasuto
Y
Uchino
Masazumi
M
Takasaki
Sin-ichi
S
Ozaki
Yukio
Y
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D003094:Collagen / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D038982:Integrin alpha2beta1 / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013928:Thromboxane A2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D038982:Integrin alpha2beta1; D010980:Platelet Membrane Glycoproteins; D017452:Receptors, IgG; C098341:platelet membrane glycoprotein VI; C538997:poly(prolyl-hydroxyprolyl-glycyl); D014443:Tyrosine; D013928:Thromboxane A2; D003094:Collagen