Liquid chromatography-mass spectrometry in in vitro drug metabolite screening.
A combination of high performance liquid chromatography (HPLC) and mass spectrometry (LC/MS) has proven its status as the most powerful analytical tool for screening and identifying drug metabolites in modern drug discovery. These techniques have become irreplaceable for drug metabolism laboratories, providing high amounts of information from a wide variety of samples. This review focuses on the most common and useful applications of these techniques when working on in vitro metabolism, more specifically with screening and identification of chemically stable or reactive metabolites formed via biotransformation reactions. Matching specific tasks and suitable instruments is a recurring consideration; for many reasons, the time-of-flight or orbitrap mass spectrometry provides clearly increased efficiency in metabolite profiling compared to other types of mass spectrometry.
Tolonen
Ari
A
Novamass Ltd., Medipolis Center, Kiviharjuntie 11, 90220 Oulu, Finland. ari.tolonen@novamass.net
Turpeinen
Miia
M
Pelkonen
Olavi
O
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055808:Drug Discovery / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism; D010599:Pharmacokinetics
D004364:Pharmaceutical Preparations