title: Distribution and density of CD1a+ and CD83+ dendritic cells in HPV-associated laryngeal papillomas.
abstract: BACKGROUND
  Respiratory papillomatosis associated with human papilloma virus (HPV) infection is the most common benign laryngeal neoplasm. The age of patients at disease onset, HPV type, number of surgeries are well known prognostic factors of the disease course. The correlation between dendritic cell (DC) density in tumor tissue and clinical prognosis was established.
  
  
  OBJECTIVE
  The aim of our study was to estimate the density of DC in laryngeal papillomas associated with HPV types 6/11 infection and to evaluate the relationship between the number of DC and the disease severity.
  
  
  METHODS
  Our study included 40 randomly selected biopsy specimens from patients with HPV-positive laryngeal papillomatosis aged from 1.7 to 20 year. DC were immunohistochemically labelled with anti-CD1a antibodies and anti-CD83 antibodies. The density of DC was analysed in epithelial layer and lamina propria.
  
  
  RESULTS
  In the epithelial layer of papillomas the number of CD1a+ and CD83+ DC was 86.2 (47.5-119.9) cells/mm(2) and 2.6 (0.6-7.9) cells/mm(2), respectively. In lamina propria - 15.3 (5.1-27.9) and 16.0 (6.7-33.2) cells/mm(2). For subgroups of patients with high number of operations (more than 3), early disease onset (children under 3 years of age) and lingering duration of disease (more than 1 year) we detected an increase of CD83+ DC in the epithelial layer. However, our data did not demonstrate a statistically significant difference in CD1a+ DC count neither in the epithelium nor in the lamina propria. Probably, the increase of CD83+ DC density in epithelial layer of patients with severe course of disease can be an evidence of impaired migration of matured DC.
authors.affiliation: Department of Pediatric Pathology, Institute of Pathology, Johannes Gutenberg University of Mainz, Mainz, Germany. mail@dr-kovalenko.com
authors.forename: Sergey
authors.initials: S
authors.lastname: Kovalenko
authors.forename: Polina
authors.initials: P
authors.lastname: Lukashenko
authors.forename: Alesia
authors.initials: A
authors.lastname: Romanovskaya
authors.forename: Iouri L
authors.initials: IL
authors.lastname: Soldatski
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Bakanov
authors.forename: Herbert
authors.initials: H
authors.lastname: Pfister
authors.forename: Valentin
authors.initials: V
authors.lastname: Gerein
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis*; D018949:Antigens, CD1 / Q000032:analysis*; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D005260:Female; D052140:Human papillomavirus 11 / Q000276:immunology; D052163:Human papillomavirus 6 / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007223:Infant; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D007830:Larynx / Q000166:cytology / Q000821:virology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D010212:Papilloma / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology; D012189:Retrospective Studies; D014018:Tissue Distribution; D055815:Young Adult; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C419393:CD1a antigen