title: Using reverse genetics to develop small knockout collections for specific biological questions.
abstract: With the advent of indexed mutagenized insertion lines in Arabidopsis, it is now possible to order small knockout collections of particular genes to probe a question of biological interest. This first requires querying Arabidopsis databases to identify lines of interest, ordering them and then verifying homozygous lines to make your collection. Once the collection is in hand, it can be used multiple times to test scientific hypotheses as they arise.
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
authors.forename: Julian
authors.initials: J
authors.lastname: Northey
authors.forename: Peter
authors.initials: P
authors.lastname: McCourt
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017343:Genes, Plant; D018745:Genome, Plant; D044404:Genomic Islands; D016254:Mutagenesis, Insertional; D010935:Plant Diseases / Q000235:genetics; D030821:Plants, Genetically Modified
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA