[Correlation between the expression of gastrocolic omentum visfatin mRNA and gestational diabetes mellitus].
OBJECTIVE
To investigate the relationship between different expression of gastrocolic omentum visfatin(VF) mRNA and gestational diabetes mellitus (GDM) in late trimester pregnancy.


METHODS
One hundred cases of pregnant women in late trimester were divided into two groups, including 45 GDM women (GDM group) and 55 normal glucose tolerance women (NGT group). Semi-quantitative RT-PCR was applied to determine the expression of VF mRNA in gastrocolic omentum. Fast-blood glucose, insulin, cholesterol (TC) and triglyceride (TG) levels were measured. The progestational body mass index (BMI) and the homeostasis model assessment (HOMA) insulin resistance (IR) were calculated in these cases.


RESULTS
The expression of gastrocolic omentum VF mRNA in GDM group and NGT group were 0.8 +/- 0.4, 0.5 +/- 0.3. Fast-blood glucose levels were (4.12 +/- 0.14), (3.65 +/- 0.13) mmol/L. Insulin levels were (72 +/- 5), (61 +/- 5) pmol/L. TG levels were (5.6 +/- 0.3), (3.8 +/- 0.3) mmol/L. TC levels were (5.6 +/- 0.9), (3.9 +/- 0.3) mmol/L. HOMA-IR were 12.5 +/- 5.9, 9.5 +/- 0.8. The progestational BMIs were (22.6 +/- 0.8), (20.9 +/- 0.4) kg/m(2). All the indices in the GDM group were significantly higher than NGT group (P < 0.05). Furthermore, the expression of gastrocolic omentum VF mRNA had a positive correlation with BMI (r = 0.32, P < 0.01), but had no association with insulin resistance or metabolic parameters.


CONCLUSIONS
The experiment demonstrates that different expression of VF mRNA has a strong correlation with GDM and obesity.
Liang
Yan
Y
Department of Obstetrics and Gynecology, First Affiliated People's Hospital, Jiaotong University, Shanghai 200080, China.
Xu
Xian-Ming
XM
Wang
Hong-Sheng
HS
Wang
Pei-Wei
PW
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D015992:Body Mass Index; D016640:Diabetes, Gestational / Q000097:blood / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism*; D009852:Omentum / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001786:Blood Glucose; D007328:Insulin; D012333:RNA, Messenger; D054409:Nicotinamide Phosphoribosyltransferase