Antiproliferative cardenolide glycosides of Elaeodendron alluaudianum from the Madagascar Rainforest.
Bioassay-guided fractionation of an ethanol extract of a Madagascar collection of Elaeodendron alluaudianum led to the isolation of two new cardenolide glycosides (1 and 2). The (1)H and (13)C NMR spectra of both compounds were fully assigned using a combination of 2D NMR experiments, including (1)H-(1)H COSY, HSQC, HMBC, and ROESY sequences. Both compounds 1 and 2 were tested against the A2780 human ovarian cancer cell line and the U937 human histiocytic lymphoma cell line assays, and showed significant antiproliferative activity with IC(50) values of 0.12 and 0.07 microM against the A2780 human ovarian cancer cell line, and 0.15 and 0.08 microM against the U937 human histiocytic lymphoma cell line, respectively.
Hou
Yanpeng
Y
Department of Chemistry, Virginia Polytechnic Institute and State University, 3111 Hahn Hall, Blacksburg, M/C 0212, VA 24061-0212, USA.
Cao
Shugeng
S
Brodie
Peggy
P
Callmander
Martin
M
Ratovoson
Fidisoa
F
Randrianaivo
Richard
R
Rakotobe
Etienne
E
Rasamison
Vincent E
VE
Rakotonandrasana
Stephan
S
TenDyke
Karen
K
Suh
Edward M
EM
Kingston
David G I
DG
D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029753:Celastraceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008270:Madagascar; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D002298:Cardenolides; D006027:Glycosides