JC viral DNA chip allows geographical localization of unidentified cadavers for rapid identification.
To narrow down the geographical origins of unidentified cadavers, a rapid and simple method to detect JC virus (JCV) genotypes using a DNA chip was developed. Fifty-four probes on a 3mm silicon DNA chip were designed to distinguish 12 JCV genotypes. The detection limit of the DNA chip was 0.001 pg/microL of template JCV DNA concentration, showing a sensitivity equal to that of the conventional method. The analysis requires only a few hours to yield results. This new DNA chip method helps to narrow the search area for human identification much more quickly and easily than use of the conventional method.
Ikegaya
Hiroshi
H
National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. ikegaya-tky@umin.ac.jp
Iwase
Hirotaro
H
Saukko
Pekka J
PJ
Akutsu
Tomoko
T
Sakurada
Koichi
K
Yoshino
Mineo
M
D002102:Cadaver; D004279:DNA, Viral / Q000235:genetics*; D018732:Forensic Anthropology / Q000379:methods*; D005838:Genotype; D005843:Geography; D006801:Humans; D007577:JC Virus / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013698:Templates, Genetic; D013997:Time Factors
D004279:DNA, Viral