title: Passive transfer of Plasmodium falciparum MSP-2 pseudopeptide-induced antibodies efficiently controlled parasitemia in Plasmodium berghei-infected mice.
abstract: We have developed monoclonal antibodies directed against the pseudopeptide psi-130, derived from the highly conserved malarial antigen Plasmodium falciparum merozoite surface protein 2 (MSP-2), for obtaining novel molecular tools with potential applications in the control of malaria. Following isotype switching, these antibodies were tested for their ability to suppress blood-stage parasitemia through passive immunization in malaria-infected mice. Some proved totally effective in suppressing a lethal blood-stage challenge infection and others reduced malarial parasitemia. Protection against P. berghei malaria following Ig passive immunization can be associated with specific immunoglobulins induced by a site-directed designed MSP-2 reduced amide pseudopeptide.
authors.affiliation: Fundación Instituto de Inmunología de Colombia-FIDIC, Bogotá, Colombia.
authors.forename: Paola A
authors.initials: PA
authors.lastname: Martínez
authors.forename: Nubia
authors.initials: N
authors.lastname: Yandar
authors.forename: Liliana P
authors.initials: LP
authors.lastname: Lesmes
authors.forename: Martha
authors.initials: M
authors.lastname: Forero
authors.forename: Oscar
authors.initials: O
authors.lastname: Pérez-Leal
authors.forename: Manuel Elkin
authors.initials: ME
authors.lastname: Patarroyo
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Lozano
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology / Q000627:therapeutic use*; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D007116:Immunization, Passive; D008288:Malaria / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D018512:Parasitemia / Q000188:drug therapy*; D010962:Plasmodium berghei; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C065974:merozoite surface protein 2, Plasmodium