title: A new method for determination of myricetin and quercetin using solid phase microextraction-high performance liquid chromatography-ultra violet/visible system in grapes, vegetables and red wine samples.
abstract: A new approach for the extraction and determination of myricetin and quercetin by using SPME-HPLC-UV system has been developed. The method involves adsorption of flavonoids on CAR/TPR fiber followed by desorption in the desorption chamber of SPME-HPLC interface using citrate buffer (0.001 M):acetonitrile (70:30) as mobile phase and UV detection at 372 nm. The detection limits for myricetin and quercetin are 48.3 and 24.7 pgmL(-1), respectively. The proposed method was validated by determining myricetin and quercetin in tomato, onion, grapes and red wine samples.
authors.affiliation: Department of Chemistry, Punjabi University, Patiala 147 002, India.
authors.forename: Ashwini
authors.initials: A
authors.lastname: Kumar
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Malik
authors.forename: Dhananjay Kumar
authors.initials: DK
authors.lastname: Tewary
mesh_terms: D000327:Adsorption; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D011794:Quercetin / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012492:Salts / Q000737:chemistry; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D013997:Time Factors; D014466:Ultraviolet Rays; D014675:Vegetables / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D005419:Flavonoids; D012492:Salts; C040015:myricetin; D011794:Quercetin