From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel.
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.
Khvorostov
Ivan
I
David Geffen School of Medicine, University of California, Los Angeles, USA.
Zhang
Jin
J
Teitell
Michael
M
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D004338:Drug Combinations; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007797:Laminin; D051379:Mice; D011509:Proteoglycans
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen