Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysis.
The highly specific molecular recognition properties of aptamers are combined with the unique optical properties of gold nanoparticles for the development of a dry-reagent strip biosensor that enables qualitative (visual)/quantitative detection of protein within minutes. A model system comprising thrombin as an analyte and a pair of aptamer probes is used to demonstrate the proof-of-concept on the conventional lateral flow test strip. The assay avoids the multiple incubation and washing steps performed in most current aptamer-based protein analyses. Although qualitative tests are realized by observing the color change of the test zone, quantitative data are obtained by recording the optical responses of the test zone with a portable "strip reader". The response of the biosensor is linear over the range of 5-100 nM of thrombin with a detection limit of 2.5 nM (S/N = 3). By comparing the analytical performances of the aptamer-based strip biosensor with the antibody-based strip sensor, we can demonstrate that aptamers are equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. The sensor was used successfully for detection of thrombin in human plasma samples. It shows great promise for use of aptamer-functionalized gold nanoparticle probes in dry-reagent strip biosensors for point-of-care or in-field detection of proteins.
Xu
Hui
H
College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China.
Mao
Xun
X
Zeng
Qingxiang
Q
Wang
Shengfu
S
Kawde
Abdel-Nasser
AN
Liu
Guodong
G
D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D011934:Reagent Strips; D013917:Thrombin / Q000032:analysis
D052157:Aptamers, Nucleotide; D011506:Proteins; D011934:Reagent Strips; D006046:Gold; D013917:Thrombin