[Study on qualitative and quantitatire methods for Ceratostigma willmottianum].
OBJECTIVE
To establish the qualitative and quantitative methods of Caratostigma willmottianum.


METHODS
The roots of Radix Ceratostigmae were identified by TLC with the authentic medicinal material and plumbagin as the reference. The contents of plumbagin in Radix Ceratostigmae were determined by HPLC.


RESULTS
The TLC method was simple and specific. A Diamonsil C18 column was used. The mobile phase was methanol-water (75:25). Plumbagin in the sawple extract was separated well. The linear range of plumbagin was 33.6-313.6 ng. The average recovery of plumbagin was 100.3% and RSD was 1.9%.


CONCLUSIONS
The methods can be used for identification and determination of plumbagin in C. willmottianum.
Wang
Ai-min
AM
School of Pharmacy, Guiyang Medical Colledge, Guiyang 550004, China.
Wang
Yong-lin
YL
Li
Yong-jun
YJ
Su
Hong
H
Zhao
Ping
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D009285:Naphthoquinones / Q000032:analysis; D029606:Plumbaginaceae / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D009285:Naphthoquinones; C014758:plumbagin