title: Efficient in vitro expansion of JC virus-specific CD8(+) T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy.
abstract: Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8(+) cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA-A0201(+) healthy controls, HIV(+) and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce a potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.
authors.affiliation: Div. of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Marzocchetti
authors.forename: Marco
authors.initials: M
authors.lastname: Lima
authors.forename: Troy
authors.initials: T
authors.lastname: Tompkins
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Kavanagh
authors.forename: Rajesh T
authors.initials: RT
authors.lastname: Gandhi
authors.forename: David W
authors.initials: DW
authors.lastname: O'Neill
authors.forename: Nina
authors.initials: N
authors.lastname: Bhardwaj
authors.forename: Igor J
authors.initials: IJ
authors.lastname: Koralnik
mesh_terms: D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology*; D015658:HIV Infections / Q000150:complications; D006801:Humans; D066298:In Vitro Techniques; D007577:JC Virus / Q000276:immunology*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000276:immunology*; D008875:Middle Aged