Metal-enhanced fluorescence of tryptophan residues in proteins: application toward label-free bioassays.
The detection of submonolayers of proteins based on native fluorescence is a potentially valuable approach for label-free detection. We have examined the possibility of using silver nanostructures to increase the emission of tryptophan residues in proteins. Fluorescence spectra, intensities, and lifetimes of multilayers and submonolayers of proteins deposited on the surfaces of silver island films were measured. Increased fluorescence intensities from two- to three-fold and similar decreases in lifetimes were observed in the presence of the silver nanoparticles compared with the proteins on the surface of the bare quartz. The observed spectral effects of silver nanoparticles on tryptophan fluorescence indicates the possibility for the design of analytical tools for the detection of proteins without traditional labeling by extrinsic fluorophores.
Szmacinski
Henryk
H
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD 21201, USA. henry@cfs.umbi.umd.edu
Ray
Krishanu
K
Lakowicz
Joseph R
JR
D005453:Fluorescence; D053768:Metal Nanoparticles; D015336:Molecular Probe Techniques; D011506:Proteins / Q000032:analysis*; D012834:Silver; D014364:Tryptophan / Q000737:chemistry*
D011506:Proteins; D012834:Silver; D014364:Tryptophan