A functional genomics approach to identify and characterize oxidation resistance genes.
In order to develop a more complete understanding of the genes required for resistance to oxidative DNA damage, we devised methods to identify genes that can prevent or repair oxidative DNA damage. These methods use the oxidative mutator phenotype of a repair deficient E. coli strain to measure the antimutator effect resulting from the expression of human cDNAs. The method can be adapted to characterize the function, and to determine the active site domains, of putative antimutator genes. Since bacteria do not contain subcellular compartments, genes that function in mitochondria, the cytoplasm, or the nucleus can be identified. Methods to determine the localization of genes in their normal host organism are also described.
Volkert
Michael R
MR
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA.
Wang
Jen-Yeu
JY
Elliott
Nathan A
NA
D001681:Biological Assay; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005796:Genes; D023281:Genomics / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D009930:Organic Chemicals / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013194:Staining and Labeling
D018076:DNA, Complementary; D024101:Mitochondrial Proteins; C419777:OXR1 protein, human; D009930:Organic Chemicals; D011506:Proteins; D017382:Reactive Oxygen Species; C107472:red dye CMXRos; D006861:Hydrogen Peroxide