Effect of polygonum multiflorum on the fluidity of the mitochondria membrane and activity of COX in the hippocampus of rats with Abeta 1-40-induced Alzheimer's disease.
OBJECTIVE
To explore the effect of polygonum multiflorum on the fluidity of mitochondria membrane and activity of cytochrome oxidase (COX) in Alzheimer's disease (AD) model rats.


METHODS
Forty-five SD rats were randomly divided into 3 groups: an AD model group, a control group, and a treatment group (n=15). AD model was established by injecting beta-amyloid protein (Abeta) 1-40 into the hippocampus of rats. The learning and memory abilities of rats were tested with the Y-electrical maze. The coefficient of viscosity of the hippocampal mitochondria membrane was determined by a spectrofluorometer, and the activity of COX was measured by an ultraviolet spectrophotometer.


RESULTS
Compared with the control group, the learning and memory ability of the AD model group was significantly lower (P<0.01), while the coefficient of viscosity of the hippocampal mitochondria membrane of the AD model group rats was significantly higher (P<0.01), and COX activity was lower (P<0.01). Compared with the AD model group rats, the coefficient of viscosity of the hippocampal mitochondria membrane of the treatment group was significantly lower (P<0.05), and COX activity was significantly improved (P<0.05).


CONCLUSIONS
Polygonum multiflorum could improve the fluidity of mitochondria membrane and the activity of mitochondrial COX in the model of Alzheimer's disease.
Hou
De-ren
DR
Department of Neurology, Third Xiangya Hospital, Central South University, Changsha 410013, China. hou0718@xy3yy.com
Wang
Yan
Y
Xue
Li
L
Tian
Yi
Y
Chen
Kun
K
Song
Zhi
Z
Yang
Qi-dong
QD
D000544:Alzheimer Disease / Q000139:chemically induced / Q000378:metabolism*; D016229:Amyloid beta-Peptides; D000818:Animals; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects; D010446:Peptide Fragments; D028502:Polygonum / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D008565:Membrane Proteins; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D051545:Cyclooxygenase 1; C496513:Ptgs1 protein, rat