title: Enumeration of bacteriophages by double agar overlay plaque assay.
abstract: The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.
authors.affiliation: Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: Amanda
authors.initials: A
authors.lastname: Mazzocco
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Waddell
authors.forename: Erika
authors.initials: E
authors.lastname: Lingohr
authors.forename: Roger P
authors.initials: RP
authors.lastname: Johnson
mesh_terms: D000362:Agar; D001435:Bacteriophages / Q000254:growth & development* / Q000302:isolation & purification*; D010948:Viral Plaque Assay / Q000295:instrumentation / Q000379:methods*
chemical_list: D000362:Agar