title: Genetic characterization of two low pathogenic avian influenza virus H5N1 isolates from Ontario, Canada.
abstract: Genomes of two low pathogenic H5N1 avian influenza (LPAI) viruses, A/Turkey/ON/84/1983 and A/Mallard/ON/499/2005 from Ontario, Canada were cloned and genetically characterized. Phylogenetic analysis showed that the Canadian isolates cluster with other North American AIVs and are distinct from the Euro-Asian H5N1 isolates. Individual gene comparisons demonstrated that the Ontario isolates were most similar to the viruses isolated from around the same time period and geographical area. A long deletion of 22 amino acids was identified in the stalk region of NA of A/Turkey/ON/84/1983 isolate, a characteristic mutation related to its adaptation to domestic birds. To our knowledge A/Turkey/ON/84/1983 genomic sequence is the first and only available entire genomic sequence of a H5N1 AIV from domestic birds in Canada and USA.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada, N1G 2W1.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Pei
authors.forename: Janet
authors.initials: J
authors.lastname: Swinton
authors.forename: Davor
authors.initials: D
authors.lastname: Ojkic
authors.forename: Shayan
authors.initials: S
authors.lastname: Sharif
mesh_terms: D000818:Animals; D046090:Anseriformes / Q000821:virology; D016000:Cluster Analysis; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology; D008969:Molecular Sequence Data; D009864:Ontario; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D014422:Turkeys / Q000821:virology
chemical_list: D012367:RNA, Viral