title: Binding assays for abscisic acid receptors.
abstract: The plant hormone abscisic acid (ABA) is a major growth regulator mediating several aspects of plant development and stress-induced processes. For the hormone to function within the cell, it must first interact with a proteinaceous receptor to commence transmission of a signal to downstream components that elicit a cellular response. With the identification of several ABA receptors, there is an increased interest in receptor-ABA interaction studies and a demand for easy-to-follow protocols to allow measurement and quantification of ABA binding. Here, we present protocols for radioligand-binding measurements with particular focus on the ABA-binding of the recently identified ABA receptors, ABAP1 and FCA.
authors.affiliation: Plant Science Department, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Fawzi A
authors.initials: FA
authors.lastname: Razem
authors.forename: Robert D
authors.initials: RD
authors.lastname: Hill
mesh_terms: D000040:Abscisic Acid / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods*
chemical_list: D010940:Plant Proteins; D000040:Abscisic Acid