Determination of acid alpha-naphthyl acetate esterase enzyme activity in peripheral blood leukocytes of gazelles (Gazella subgutturosa).
We examined gazelle peripheral blood leucocytes using the alpha-Naphthyl acetate esterase (ANAE) staining technique (pH 5.8). Our purpose was to determine the percentage of ANAE positive lymphocytes. The proportion of ANAE positive T-lymphocytes was 72%. T-lymphocytes showed an ANAE positive reaction, but eosinophilic granulocytes and monocytes also showed a positive reaction. By contrast, no reaction was detected in B-lymphocytes, neutrophil granulocytes or platelets. The reaction observed in T-lymphocytes was a red-brown coloration, usually 1-2 granules, but enough granules to fill the cytoplasm were detected rarely. As a result of ANAE enzyme staining, we concluded that the staining technique can be used as a cytochemical marker for gazelle T-lymphocytes.
Altunay
H
H
Department of Histology and Embryology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur. altunay@mehmetakif.edu.tr
Harem
I S
IS
Harem
M K
MK
Asti
R N
RN
Kurtdede
N
N
D000818:Animals; D001792:Blood Platelets / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology*; D009283:Naphthol AS D Esterase / Q000032:analysis* / Q000378:metabolism*; D012418:Ruminants / Q000097:blood*
D009283:Naphthol AS D Esterase