[Apoptosis induced by NNAMB, a novel polyamine conjugate, in human erythroleukemia K562 cells and its mechanism].
OBJECTIVE
To investigate the apoptosis-inducing effects of NNAMB, a novel polyamine conjugate, in erythroleukemia K562 cells and its molecular mechanism.


METHODS
Cell viability was assessed by MTT assay and trypan blue dye exclusion method. The cell morphology was observed by fluorescence microscopy. The cell cycle distribution, apoptosis and mitochondrial membrane potential were measured by flow cytometry. The expression of caspase-3, -8, -9, cytochrome c in the K562 cells was detected by Western blot.


RESULTS
NNAMB inhibited the proliferation of K562 cells. The cells treated with NNAMB showed a typical apoptotic morphology, Sub-G1 peak and loss of mitochondrial membrane potential. Western blot assay showed that NNAMB increased the expression of caspase-3, -9, cytochrome c but not caspase-8 in a dose-and time-dependent manner.


CONCLUSIONS
NNAMB induces apoptosis via mitochondrial pathway in K562 cells.
Xie
Song-Qiang
SQ
Department of Pharmacology, Henan University, Kaifeng 475001, China.
Wu
Ying-Liang
YL
Liu
Guang-Chao
GC
Cheng
Peng-Fei
PF
Wang
Min-Wei
MW
Ma
Yuan-Fang
YF
Zhao
Jin
J
Wang
Chao-Jie
CJ
D000873:Anthracenes / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D011073:Polyamines / Q000494:pharmacology*; D013095:Spermidine / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000873:Anthracenes; C548263:NNAMB compound; D011073:Polyamines; D045304:Cytochromes c; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D013095:Spermidine