High throughput screening various abused drugs and metabolites in urine by liquid chromatography-heated electrospray ionization/tandem mass spectrometry.
An integrated method of liquid chromatography-heated electrospray ionization/tandem mass spectrometry was evaluated for high throughput screening of various abused drugs in urine. Chromatographic analysis was performed on a C18 reverse phase column using a linear gradient of 10mM ammonium acetate containing 0.1% formic acid-methanol as mobile phase and the total separation time was 7 min. A simple and rapid sample preparation method used was by passing urine samples through a 0.22 microm PVDF syringe filter. The detection limits of the studied abused drugs in urine were from 0.6 ng mL(-1) (ketamine) to 9.0 ng mL(-1) (norcodeine). According to the results, the linear range was from 1 to 1200 ng mL(-1) with relative standard deviation (R.S.D.s) value below 14.8% (intra-day) and 24.6% (inter-day). The feasibility of applying the proposed method to determine various abused drugs in real samples was examined by analyzing urine samples from drug-abused suspects. The abused drugs including ketamines and amphetamines were detected in suspected urine samples. The results demonstrate the suitability of LC-HESI-MS/MS for high throughput screening of the various abused drugs in urine.
Chen
Chung-Yu
CY
Department of Chemistry, National Chung Hsing University, Taichung, Taiwan, ROC.
Shen
Chien-Chun
CC
Yang
Tzung-Jie
TJ
Chang
Yan-Zin
YZ
Lee
Maw-Rong
MR
D000085:Acetates / Q000032:analysis; D000662:Amphetamines / Q000032:analysis; D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D005561:Formates / Q000032:analysis; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D007649:Ketamine / Q000032:analysis; D000432:Methanol / Q000032:analysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D013997:Time Factors; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*
D000085:Acetates; D000662:Amphetamines; D005561:Formates; D013287:Illicit Drugs; C030544:formic acid; D007649:Ketamine; C018824:ammonium acetate; D000432:Methanol