Human stem cell factor (SCF) is a heparin-binding cytokine.
Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmin, but not others. The binding of SCF was also inhibited with 0.55 M or higher concentrations of NaCl in the medium. These results suggested that a high content of all three sulfate groups in repeating disaccharide units is required for interaction with SCF. Furthermore, pre-immobilized SCF on F/P MP-coated plates significantly stimulated proliferation of a human erythroleukemia cell line.
Kishimoto
Satoko
S
Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan.
Nakamura
Shingo
S
Hattori
Hidemi
H
Nakamura
Shin-Ichiro
S
Oonuma
Fumie
F
Kanatani
Yasuhiro
Y
Tanaka
Yoshihiro
Y
Mori
Yasutaka
Y
Harada
Yasuji
Y
Tagawa
Masahiro
M
Ishihara
Masayuki
M
D004187:Disaccharides / Q000032:analysis; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding / Q000187:drug effects; D019089:Stem Cell Factor / Q000378:metabolism* / Q000494:pharmacology
D004187:Disaccharides; D019089:Stem Cell Factor; D006493:Heparin