[Studies on bioactive constituents of whole herbs of Vernonia cinerea].
OBJECTIVE
To study the constituents of the whole herbs of Vernonia cinerea. by bio-activity guided isolation with PC-12 model.


METHODS
The constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods.


RESULTS
Four compounds were identified to be (+)-lirioresinol B (1), stigmasterol (2), stigmasterol-3-O-beta-D-glucoside (3), 4-sulfo-benzocyclobutene (4), and their NGF inducing activity were also investigated.


CONCLUSIONS
Compounds 1, 3, 4 were isolated from this genus for the first time, and compound 4 was identified as a new natural product. Compounds 1, 3, 4 showed cytotoxicity on PC-12, and compounds 2, 3, 4 showed inhibition activity. Compound 4 showed a specific effect on the survival of TrkA fibroblasts, and resulted in the inducing NGF activity.
Zhu
Hua-xu
HX
School of life sciences, Nanjing Normal University, Nanjing 210024, China. Huaxu72@yahoo.com.cn
Tang
Yu-ping
YP
Pan
Lin-mei
LM
Min
Zhi-da
ZD
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D016716:PC12 Cells; D011083:Polycyclic Compounds / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000737:chemistry; D035741:Vernonia / Q000737:chemistry*
C540437:4-sulfo-benzocyclobutene; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D011083:Polycyclic Compounds; C464587:stigmasterol glucoside; D013265:Stigmasterol