title: A newly identified bocavirus species in human stool.
abstract: Viral metagenomic analysis was used to identify a previously uncharacterized parvovirus species, "HBoV2," whose closest phylogenetic relative is the human bocavirus (HBoV). HBoV2 has a genomic organization identical to that of HBoV but has only 78%, 67%, and 80% identity, respectively, with the latter's NS1, NP1, and VP1/VP2 proteins. The study used polymerase chain reaction to detect HBoV2 sequences in 5 of 98 stool samples from Pakistani children and in 3 of 699 stool samples from Edinburgh. Nearly-full-length genome sequencing revealed the presence of 3 HBoV2 genotypes and evidence of recombination between genotypes. Further studies are necessary to identify anatomical sites of HBoV2 replication and potential associations with clinical symptoms or disease.
authors.affiliation: Blood Systems Research Institute and Department of Laboratory Medicine, University of California, San Francisco, CA 94118, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Kapoor
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Slikas
authors.forename: Peter
authors.initials: P
authors.lastname: Simmonds
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Chieochansin
authors.forename: Asif
authors.initials: A
authors.lastname: Naeem
authors.forename: Shahzad
authors.initials: S
authors.lastname: Shaukat
authors.forename: Muhammad Masroor
authors.initials: MM
authors.lastname: Alam
authors.forename: Salmaan
authors.initials: S
authors.lastname: Sharif
authors.forename: Mehar
authors.initials: M
authors.lastname: Angez
authors.forename: Sohail
authors.initials: S
authors.lastname: Zaidi
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D052660:Bocavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D010154:Pakistan / Q000453:epidemiology; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity