[Effects of quercetin and quercetin in combination with cisplatin on adhesion, migration and invasion of HeLa cells].
OBJECTIVE
To explore the effects of quercetin and quercetin in combination with cisplatin on adhesion, migration and invasion of HeLa cells.


METHODS
Adhesion, migration and invasion of HeLa cells treated with quercetin and quercetin in combination with cisplatin were measured by adhesion assay, wound healing assay, and transwell chamber method respectively.


RESULTS
The results showed that quercetin and quercetin in combination with cisplatin could inhibit adhesion, migration and invasion of HeLa cells. The adhesive ratio, migration rate and the invasiveness were negatively proportional to concentration of quercetin and quercetin in combination with cisplatin. With increasing concentration of quercetin from 20 to 80 micromol/L, the adhesive ratio decreased from (82.2 +/- 1.5)% to (48.4 +/- 1.1)%; the migration rate decreased from (7.26 +/- 0.20) microm/h to (3.78 +/- 0.64) microm/h; the invasiveness decreased from (124.3 +/- 1.5) piece to (90.7 +/- 2.1) piece (P < 0.05). In quercetin and quercetin in combination with 10 micromol/L cisplatin treatment group, with quercetin concentration increasing from 20 to 80 micromol/L, the adhesive ratio decreased from (42.6 +/- 1.2)% to (27.5 +/- 1.7)%, the migration rate decreased from (2.20 +/- 0.33) microm/h to (0.72 +/- 0.19) microm/h and the invasiveness decreased from (78.7 +/- 2.5) piece to (44.0 +/- 4.0) piece (P < 0.05). Compared with the quercetin groups, quercetin in combination with cisplatin groups had significantly higher inhibitory effects (P < 0.05).


CONCLUSIONS
Quercetin and quercetin in combination with cisplatin can inhibit adhesion and migration and invasion of HeLa cells. Quercetin can enhance the inhibitory effect of cisplatin on HeLa cell adhesion, migration and invasion.
Zhang
Feng-Li
FL
Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University, Wuhan 430060, China.
Zhang
Wei
W
Chen
Xiao-Mei
XM
Luo
Ruo-Yu
RY
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D016896:Treatment Outcome
D000972:Antineoplastic Agents, Phytogenic; D011794:Quercetin; D002945:Cisplatin