title: Polymorphism of the bovine POU1F1 gene: allele frequencies and effects on milk production in three Iranian native breeds and Holstein cattle of Iran.
abstract: The aim of this study was to estimate the allele frequencies in polymorphic site of exon six of POU1F1 gene in three Iranian native and Holstein cattle. Genomic DNA was extracted from 3 Iranian native cattle breeds, including 97 Mazandarani, 87 Sarabi, 112 Golpaygani and also 110 Holstein cattle. A 451 bp fragment of intron 5 and exon 6 were amplified and digested with HinfI restriction enzyme. Frequencies of allele A were 0.37, 0.27, 0.34 and 0.21 for Mazandarani, Sarabi, Golpaygani and Holstein cattle, respectively. Significant differences in genotype frequencies were found between Mazandarani or Golpaygani and Holstein cattle. No significant differences in genotype frequencies were found between Sarabi and Holstein cattle. Transition A to G in nucleotide 1256 is responsible for HinfI(-) allele. No significant association was observed between POU1F1 polymorphism and milk production. Differences in allelic frequency between native Bos indicus breeds (Mazandarani, Golpaygani) and Holstein at the present study might be due to differences in origin breeds, low number of samples and/or as the effect of natural selection in native breeds.
authors.affiliation: Department of Animal Science, Hasheminejad High Education Center, Mashhad, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Zakizadeh
authors.forename: M
authors.initials: M
authors.lastname: Reissmann
authors.forename: G
authors.initials: G
authors.lastname: Rahimi
authors.forename: A Nejati
authors.initials: AN
authors.lastname: Javaremi
authors.forename: P
authors.initials: P
authors.lastname: Reinecke
authors.forename: S R
authors.initials: SR
authors.lastname: Mirae-Ashtiani
authors.forename: M Moradi
authors.initials: MM
authors.lastname: Shahrbabak
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007774:Lactation / Q000235:genetics*; D008892:Milk; D011110:Polymorphism, Genetic; D011247:Pregnancy; D050817:Transcription Factor Pit-1 / Q000235:genetics*
chemical_list: D050817:Transcription Factor Pit-1