Expression of integrin subunits in the herniated intervertebral disc.
Integrins are a class of cell adhesion molecules that regulate interactions between cells and their extracellular matrix (ECM). Several specific integrin receptors have been identified in intervertebral discs, including the fibronectin-binding integrin receptors alpha(5) beta(1), alpha(v) beta(3) and the collagen-binding integrin receptors alpha(1) beta(1), alpha(2) beta(1), and, alpha(v) beta(1). But the integrins expressions in degenerated intervertebral discs are still unknown. In our study, the expressions of alpha(1), alpha(2), alpha(5), alpha(v), beta(1), beta(3) integrin subunits, collagens, and fibronectin in normal and herniated intervertebral discs of human were determined. Specimens of human lumbar intervertebral discs were divided into 3 groups: normal discs (n = 10), protrusion (n = 15), and extrusion (n = 15). Real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunoprecipitation were used to evaluate the alpha(1), alpha(2), alpha(5),alpha(v), beta(1), and beta(3) integrin subunits messenger ribonucleic acid (mRNA) and protein expressions. RT-PCR was also performed to measure the mRNA level of collagen I, collagen II, and fibronectin. The expressions of alpha(5) and beta(1) subunits were increased in herniated discs, especially in the discs of extrusion. But as to alpha(1), alpha(2), alpha(v) and beta(3), their expressions had no difference among the discs. Fibronectin, whose binding integrin receptor was alpha(5) beta(1) was also increased. And in herniated discs, the collagen I was increased, but the collagen II was decreased. The expressions of some integrin subunits were increased in herniated discs. These results may be attributed to the interaction between cells and the ECM in the process of degeneration.
Xia
Maosheng
M
Department of Orthopaedics, The First Hospital of China Medical University, Shenyang, PR China.
Zhu
Yue
Y
D000328:Adult; D003094:Collagen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000096:biosynthesis*; D007403:Intervertebral Disc / Q000378:metabolism*; D007405:Intervertebral Disc Displacement / Q000378:metabolism*; D008875:Middle Aged; D021122:Protein Subunits / Q000096:biosynthesis
D005353:Fibronectins; D016023:Integrins; D021122:Protein Subunits; D003094:Collagen