Electrochemical sensor highly selective for estradiol valerate determination based on a modified carbon paste with iron tetrapyridinoporphyrazine.
This work reports the use of iron tetrapyridinoporphyrazine (FeTPyPz) as a highly selective catalyst in the construction of an electrochemical sensor for estradiol valerate (EV) determination. The sensor was prepared by modifying a carbon paste with FeTPyPz. The best results were obtained in a mixture of acetonitrile (MeCN) and 0.1 mol L(-1) phosphate buffer solution (pH 6.0) in a volume ratio of 47 : 53. A linear response range was observed between 45 and 450 micromol L(-1) with a sensitivity of 12160 +/- 306 microA L mol(-1) and quantification and detection limits of 45 and 13 micromol L(-1), respectively. The repeatability, expressed as the relative standard deviation (RSD) for n = 10, was 5.9% ([EV] = 50 micromol L(-1)). The reproducibility (RSD) for the sensor construction was better than 4% and the operational stability (RSD) over 50 measurements was 1.8%. A detailed investigation regarding the selectivity and electrochemical characteristics was carried out. Finally, in a first step to evaluate the application potential of the sensor, it was successfully applied to determine EV in a commercial formulation.
Batista
Isabelle V
IV
Universidade São Francisco, Bragança Paulista, SP, Brazil. abbaspour@chem.susc.ac.ir
Lanza
Marcos R V
MR
Dias
Iara L T
IL
Tanaka
Sônia M C N
SM
Tanaka
Auro A
AA
Sotomayor
Maria D P T
MD
D002021:Buffers; D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008665:Metalloporphyrins / Q000737:chemistry; D008839:Microelectrodes; D012997:Solvents
D002021:Buffers; D008665:Metalloporphyrins; D012997:Solvents; C541203:iron tetrapyridinoporphyrazine; D004958:Estradiol; D002244:Carbon