title: Ovine colostrum nanopeptide affects amyloid beta aggregation.
abstract: A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the PRP complex components can affect aggregation of amyloid beta (Abeta1-42). The effect of NP on Abeta aggregation was studied using Thioflavin T (ThT) binding, atomic force microscopy, and analyzing circular dichroism spectra. Results presented suggest that NP can directly interact with amyloid beta, inhibit its aggregation and disrupt existing aggregates acting as a beta sheet breaker and reduce toxicity induced by aggregated forms of Abeta.
authors.affiliation: Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. janusz@immuno.iitd.pan.wroc.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Janusz
authors.forename: Mirosław
authors.initials: M
authors.lastname: Woszczyna
authors.forename: Marek
authors.initials: M
authors.lastname: Lisowski
authors.forename: Adriana
authors.initials: A
authors.lastname: Kubis
authors.forename: Józefa
authors.initials: J
authors.lastname: Macała
authors.forename: Teodor
authors.initials: T
authors.lastname: Gotszalk
authors.forename: Józef
authors.initials: J
authors.lastname: Lisowski
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D052160:Benzothiazoles; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D005456:Fluorescent Dyes / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D017433:Protein Structure, Secondary / Q000187:drug effects; D051381:Rats; D034561:Sheep, Domestic / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D009842:Oligopeptides; D010446:Peptide Fragments; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C425383:valyl-glutamyl-seryl-tyrosyl-valyl-prolyl-leucyl-phenylalanylproline; C009462:thioflavin T