title: In silico identification of genes in bacteriophage DNA.
abstract: One of the most satisfying aspects of a genome sequencing project is the identification of the genes contained within it.These are of two types: those which encode tRNAs and those which produce proteins. After a general introduction on the properties of protein-encoding genes and the utility of the Basic Local Alignment Search Tool (BLASTX) to identify genes through homologs, a variety of tools are discussed by their creators. These include for genome annotation: GeneMark, Artemis, and BASys; and, for genome comparisons: Artemis Comparison Tool (ACT), Mauve, CoreGenes, and GeneOrder.
authors.affiliation: Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: Mark
authors.initials: M
authors.lastname: Borodovsky
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Carver
authors.forename: Ana M
authors.initials: AM
authors.lastname: Cerdeño-Tárraga
authors.forename: Aaron
authors.initials: A
authors.lastname: Darling
authors.forename: Alexandre
authors.initials: A
authors.lastname: Lomsadze
authors.forename: Padmanabhan
authors.initials: P
authors.lastname: Mahadevan
authors.forename: Paul
authors.initials: P
authors.lastname: Stothard
authors.forename: Donald
authors.initials: D
authors.lastname: Seto
authors.forename: Gary
authors.initials: G
authors.lastname: Van Domselaar
authors.forename: David S
authors.initials: DS
authors.lastname: Wishart
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D012984:Software
chemical_list: D004279:DNA, Viral