Deglycosylation of apo B-containing lipoproteins increase their ability to aggregate and to promote intracellular cholesterol accumulation in vitro.
Sub-fractions of all apo B-100 containing lipoproteins (low density lipoproteins, very low density lipoproteins and intermediate density lipoproteins) with reduced contents of sialic acid were found in vivo in human blood. These lipoproteins were inclined to spontaneously form aggregates and were able to stimulate accumulation of cholesterol in cells cultured from human aortic intima. In vitro treatment of apo B-containing lipoproteins with 2,6- and 2,3-specific sialidases, alpha-mannosidase, endoglycosidases F1 or F2 or peptide-N-glycanase F also stimulated aggregation and increased the ability of these particles to potentiate cholesterol accumulation in cells of the intact human aortic intima. So, deglycosylation of various apo B-containing lipoproteins possibly occurs in the blood, decreases their resistance to aggregation and increases the ability of these particles to stimulate accumulation of cholesterol in human aortic intima cells, thereby increasing their atherogenic potential.
Aksenov
D V
DV
Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow, Russia. denaks@mail.ru
Medvedeva
L A
LA
Skalbe
T A
TA
Sobenin
I A
IA
Tertov
V V
VV
Gabbasov
Z A
ZA
Popov
E V
EV
Orekhov
A N
AN
D000328:Adult; D053299:Apolipoprotein B-100 / Q000378:metabolism*; D050197:Atherosclerosis / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008358:Mannose / Q000378:metabolism; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011485:Protein Binding
D053299:Apolipoprotein B-100; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D002784:Cholesterol; D006026:Glycoside Hydrolases; D019158:N-Acetylneuraminic Acid; D008358:Mannose