Butyricicoccus pullicaecorum gen. nov., sp. nov., an anaerobic, butyrate-producing bacterium isolated from the caecal content of a broiler chicken.
Five isolates that produced large amounts of butyrate were obtained in the course of a study on the butyrate-producing microbiota from the caecal content of a 4-week-old broiler chicken. The five isolates were virtually indistinguishable in biochemical and genetic terms, suggesting that they were derived from a single bacterial clone colonizing this habitat. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the five isolates represented a unique lineage within the Clostridium leptum subgroup of the clostridia, with Eubacterium desmolans as the closest phylogenetic neighbour (about 93 % similarity). These data indicate that the five novel isolates represent a single novel species within a novel genus, for which we propose the name Butyricicoccus pullicaecorum gen. nov., sp. nov. The type strain of Butyricicoccus pullicaecorum is 25-3(T) (=LMG 24109(T) =CCUG 55265(T)). The DNA G+C content of strain 25-3(T) was 54.5 mol% .
Eeckhaut
Venessa
V
Department of Pathology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. venessa.eeckhaut@UGent.be
Van Immerseel
Filip
F
Teirlynck
Emma
E
Pasmans
Frank
F
Fievez
Veerle
V
Snauwaert
Cindy
C
Haesebrouck
Freddy
F
Ducatelle
Richard
R
Louis
Petra
P
Vandamme
Peter
P
D000693:Anaerobiosis; D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D002645:Chickens / Q000382:microbiology*; D005285:Fermentation; D005766:Gastrointestinal Contents / Q000382:microbiology*; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D002087:Butyrates; D012336:RNA, Ribosomal, 16S