Method for conducting microarray study of oxidative stress induced gene expression.
Cellular systems produce reactive oxygen species during the process of metabolism. Oxidative stress results in the activation or repression of many genes in important signaling pathways. DNA microarrays allow for a high throughput evaluation of the changes in gene expression levels in any biological system. In this study, we describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria.
Subbaram
Sita
S
Center for Immunology & Microbial Disease, Albany College of Medicine, Albany, NY, USA.
Melendez
Juan A
JA
Chittur
Sridar V
SV
D045744:Cell Line, Tumor; D004586:Electrophoresis; D005434:Flow Cytometry; D005452:Fluoresceins; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016877:Oxidants / Q000032:analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018075:RNA, Complementary / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
D005452:Fluoresceins; D016877:Oxidants; D018075:RNA, Complementary; D012333:RNA, Messenger; C029569:diacetyldichlorofluorescein