Influence of negative pressure when harvesting adipose tissue on cell yield of the stromal-vascular fraction.
Adipose tissue is the standard autologous filling material used in plastic surgery today. At the same time it is also a source of mesenchymal stem cells, situated in the Stromal-Vascular Fraction (SVF) and easy to obtain in large quantities. The method of harvesting adipose tissue is an important stage for cell survival. So far, comparative studies on harvesting techniques have only concerned MTT cell viability of mature adipocytes. The aim of our study was to determine the influence of pressure on the yield of SVF cells in relation to the syringe aspiration technique which is the standard technique in plastic surgery. For this, six different harvesting conditions were tested on 3 patients. For each condition, a sample was taken from the trochanter region with the help of a 3 mm cannula, manual aspiration by a 10 ml syringe; wall suction; the traditional pump suction at -350 and -700 mmHg; the power assisted liposuction at -350 and -700 mmHg. Cell yield with a pressure of -350 mmHg, assisted or not, was greater than that obtained at -700 mmHg and significantly superior to aspiration with a syringe (p<0.05). At -350 mmHg, the use of power-assisted liposuction gave better results for two out of three patients when compared to non-power-assisted liposuction. Negative pressure is a factor influencing the number of SVF cells harvested.
Mojallal
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A
Department of Plastic, Reconstructive and Aesthetic Surgery, Edouard Herriot Hospital, Lyon, France. dr.mojallal@gmail.com
Auxenfans
C
C
Lequeux
C
C
Braye
F
F
Damour
O
O
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000328:Adult; D002452:Cell Count; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005260:Female; D006801:Humans; D008297:Male; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014618:Vacuum