title: IL-17 producing CD4+ T cells mediate accelerated ischemia/reperfusion-induced injury in autoimmunity-prone mice.
abstract: Elements of the innate and adaptive immune response have been implicated in the development of tissue damage after ischemic reperfusion (I/R). Here we demonstrate that T cells infiltrate the intestine of C57BL/6 mice subjected to intestinal I/R during the first hour of reperfusion. The intensity of the T cell infiltration was higher in B6.MRL/lpr mice subjected to intestinal I/R and reflected more severe tissue damage than that observed in control mice. Depletion of T cells limited I/R damage in B6.MRL/lpr mice, whereas repletion of B6.MRL/lpr lymph node-derived T cells into the I/R-resistant Rag-1(-/-) mouse reconstituted tissue injury. The tissue-infiltrating T cells were found to produce IL-17. Finally, IL-23 deficient mice, which are known not to produce IL-17, displayed significantly less intestinal damage when subjected to I/R. Our data assign T cells a major role in intestinal I/R damage by virtue of producing the pro-inflammatory cytokine IL-17.
authors.affiliation: Department of Medicine, Uniformed Services University for the Health Sciences, Bethesda, MD 20814, USA.
authors.forename: Colin
authors.initials: C
authors.lastname: Edgerton
authors.forename: José C
authors.initials: JC
authors.lastname: Crispín
authors.forename: Chantal M
authors.initials: CM
authors.lastname: Moratz
authors.forename: Estelle
authors.initials: E
authors.lastname: Bettelli
authors.forename: Mohamed
authors.initials: M
authors.lastname: Oukka
authors.forename: Milomir
authors.initials: M
authors.lastname: Simovic
authors.forename: Athina
authors.initials: A
authors.lastname: Zacharia
authors.forename: Ryan
authors.initials: R
authors.lastname: Egan
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Jurandir J
authors.initials: JJ
authors.lastname: Dalle Lucca
authors.forename: Yuang-Taung
authors.initials: YT
authors.lastname: Juang
authors.forename: George C
authors.initials: GC
authors.lastname: Tsokos
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000503:physiopathology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D020381:Interleukin-17 / Q000276:immunology*; D007422:Intestines / Q000276:immunology / Q000293:injuries / Q000503:physiopathology; D007511:Ischemia / Q000276:immunology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D018345:Mice, Knockout; D015427:Reperfusion Injury / Q000276:immunology / Q000503:physiopathology*
chemical_list: D020381:Interleukin-17