Derived chromatographic indices as effective tools to study the self-aggregation process of bile acids.
Reversed-phase high-performance liquid chromatography (RP-HPLC) is more and more adopted as a fruitful tool for the estimation of several physico-chemical properties of diverse classes of organic compounds. In this frame, derived chromatographic indices have been proposed as effective parameters to measure the lipophilicity (log P or log D) of compounds. Instead, a limited attention has been directed towards the chromatographic evaluation of the Critical Micellar Concentration (CMC), one of the most important parameters employed to study the bile acid physico-chemical profile. We have recently reported on the effectiveness of the derived chromatographic index phi(0) for the study of the self-aggregation process of bile acids. Here we show that this index is independent upon the adopted chromatographic environment so as to be instrumental for the evaluation of the hydrophobic/hydrophilic balance of bile acids. Molecular modelling studies have also been undertaken with the aim of rationalizing the experimental findings.
Natalini
Benedetto
B
Università degli Studi di Perugia, Dipartimento di Chimica e Tecnologia del Farmaco, Via del Liceo, 1, 06123 Perugia, Italy. natalini@chimfarm.unipg.it
Sardella
Roccaldo
R
Camaioni
Emidio
E
Macchiarulo
Antonio
A
Gioiello
Antimo
A
Carbone
Giovanni
G
Pellicciari
Roberto
R
D001647:Bile Acids and Salts / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D016014:Linear Models; D008823:Micelles; D008958:Models, Molecular
D001647:Bile Acids and Salts; D008823:Micelles