title: Effect of alpha-tocopherol on carbon tetrachloride intoxication in the rat liver.
abstract: Carbon tetrachloride (1 ml/kg body weight as a 1:1 mixture of CCl(4) and mineral oil) was orally administered to rats. After 12 h, the activity of plasma ALT (alanine aminotransferase) was significantly higher than that of the control group, and plasma ALT and AST (aspartate aminotransferase) activities significantly increased 24 h after CCl(4) administration. These results indicated that the necrotic process had initiated at about 12 h and developed thereafter. After 6-24 h of CCl(4) administration, the hepatic level of vitamin C, the most sensitive indicator of oxidative stress, decreased significantly, indicating that oxidative stress was significantly enhanced 6 h after CCl(4) intoxication and thereafter. Oral administration of vitamin E (1 ml/kg body weight as a 1:1 mixture of alpha-tocopherol and mineral oil) 12 h before CCl(4) administration caused a significant elevation of liver vitamin E level and ameliorated liver necrosis 24 h after CCl(4) intoxication based on plasma AST and ALT. Vitamin E also significantly restored the hepatic vitamin C concentration 12 and 24 h after CCl(4) intoxication, demonstrating that vitamin E functioned as an antioxidant. The liver vitamin E concentration was not changed by vitamin E supplementation to rats that did not receive CCl(4). This result indicated that vitamin E accumulated in the damaged liver. The activation of JNK, ERK1/2 and p38 MAPK took place 1.5 h after CCl(4) administration. Co-administration of alpha-tocopherol with CCl(4) did not affect these early changes in MAPKs.
authors.affiliation: Department of Food Science and Nutrition, Nara Women's University, Nara, Japan.
authors.forename: Chinatsu
authors.initials: C
authors.lastname: Iida
authors.forename: Kozue
authors.initials: K
authors.lastname: Fujii
authors.forename: Eriko
authors.initials: E
authors.lastname: Koga
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Washino
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Ichi
authors.forename: Kouichi
authors.initials: K
authors.lastname: Abe
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Matsura
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kojo
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000139:chemically induced / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014810:Vitamin E / Q000378:metabolism; D024502:alpha-Tocopherol / Q000378:metabolism*
chemical_list: D014810:Vitamin E; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D024502:alpha-Tocopherol; D001205:Ascorbic Acid