[Fingerprint comparison of effective parts of Ermiao wan categorized formula in anti-gouty syndrome of damp and heat].
OBJECTIVE
To study the correlation in the material foundation in compatibility of Ermiao wan categorized formulas (Ermiaowan, Sanmiaowan, Simiaowan and modified Simiaowan) and to explore its characteristics changes.


METHODS
Determined the fingerprint of the effective parts of Ermiao wan categorized formulas in anti-gouty syndrome of damp and heat and compared the composition features and compatibility changes of total peaks by establishing the HPLC fingerprint and using reference substances to identify the ingredients of the common peaks, the ascription and the source of the chromatographic peaks.


RESULTS
The effective parts of modified Simiao Wan had 60 total peaks which come from the sites of alkaloids, flavonoids, organic acids. There were some change principles in the number of total peaks and relative peak area ratio between basic formula and categorized formulas.


CONCLUSIONS
Ermiaowan categorized formulas have same substance-foundation and there are some correlations in the compatibility changes.
Li
Xin
X
Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiansu, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
Yin
Lian
L
Duan
Jin-ao
JA
Ding
An-wei
AW
D000470:Alkaloids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000627:therapeutic use*; D005419:Flavonoids / Q000737:chemistry; D006073:Gout / Q000188:drug therapy*; D006074:Gout Suppressants / Q000737:chemistry / Q000627:therapeutic use*; D006358:Hot Temperature
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006074:Gout Suppressants