title: [A novel transcriptional regulator of the phzA1 operon in Pseudomonas aeruginosa].
abstract: OBJECTIVE
  The aim was to investigate the regulator of phzA1B1C1D1E1F1(phzA1) operon in Pseudomonas aeruginosa.
  
  
  METHODS
  Mutants affecting phzA1 expression in the presence of subinhibitory concentrations of tetracyclin were selected by screening a transposon mutagenesis library. The transposon insertion sites in the mutants were determined by arbitrary PCR and subsequent PCR product sequencing.
  
  
  RESULTS
  We found two mutants affecting the phzA1 operon expression. The transposon insertion sites in these two mutants were at the promoter region of gene PA0487 encoding a probable molybdenum transport regulator. Some genes involved in quorum sensing were also down-regulated in the mutant.
  
  
  CONCLUSIONS
  PA0487 was involved in regulating the expression of phzA1, possibly through the quorum sensing system.
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China. kongliping0516@163.com
authors.forename: Kong
authors.initials: K
authors.lastname: Liping
authors.forename: Liang
authors.initials: L
authors.lastname: Haihua
authors.forename: Dong
authors.initials: D
authors.lastname: Zhaolin
authors.forename: Duan
authors.initials: D
authors.lastname: Kangmin
authors.forename: Shen
authors.initials: S
authors.lastname: Lixin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016254:Mutagenesis, Insertional; D009876:Operon; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D053038:Quorum Sensing; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors