[Effect of verapamil on pharmacokinetics of puerarin in rats].
OBJECTIVE
To investigate the effect of verapamil on the pharmacokinetics of puerarin in rats.


METHODS
Puerarin with or without verapamil was administered intravenously or orally to rats. The concentration of puerarin in serum was determined by HPLC.


RESULTS
No significant difference was found between the control and 0.5 microg x g(-1) verapamil combined groups for intravenous administration, and there was significant difference between the control and 2. 5 microg x g(-1) verapamil combined groups (P < 0.05). When puerarin was administered orally with verapamil, significant difference was found between the control and combined groups (P < 0.05).


CONCLUSIONS
Verapamil inhibited puerarin metabolism when puerarin was coadministered with verapamil, so it is necessary to change the therapeutic dose of puerarin.
Cui
Sheng-miao
SM
Department of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China. cuishengmiao@yahoo.com
Ma
Yan-bin
YB
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004347:Drug Interactions; D007529:Isoflavones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D014665:Vasodilator Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology*
D007529:Isoflavones; D014665:Vasodilator Agents; D014700:Verapamil; C033607:puerarin