[Effect of different choices and treatments with donor cells on reprogramming].
The donor nucleus must experienced the epigenetic modification of the process reprogramming and went back to the initial state after the donor cell was injected into the oocytes. If the reprogramming is not completed, the efficiency of cloning will be reduced. However, reprogramming of nucleus muct was not only embodied in its ability after it was transferred into the oocytes. It was different in the potential if the cell type was not identical. In addition, different treatment to the donor cells resulted in different ability and the level of reprogramming. This paper described different effects of the type, algebra, cycles, age, and species of the donor cells after nuclear transplantation on the reprogramming. An overview of the exposition and analysis through the donor cell cryopreservation, serum starvation, and different reagent treatments were discussed.
Yao
Ya-Xin
YX
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China.
Li
Xiang-Chen
XC
Zhang
Yong
Y
Qiao
Li-Min
LM
Guan
Wei-Jun
WJ
Ma
Yue-Hui
YH
D000818:Animals; D002453:Cell Cycle; D002477:Cells / Q000166:cytology* / Q000378:metabolism; D065150:Cellular Reprogramming; D016922:Cellular Senescence; D015925:Cryopreservation; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism
D007202:Indicators and Reagents