[Reactivity of five different antibodies with benign and malignant melanocytic lesions].
At the histological examination of an increasing number of melanocytic tumors there is a need to use various immunohistochemical methods. Currently, we are supplied by several antibodies working well on formalin-fixed, paraffin-embedded samples. We have tested five antibodies (S-100, HMB-45, Melan-A, MITF, PNL-2) on 34 benign and 34 malignant melanocytic tumors. We examined the specificity and sensitivity in the junctional and dermal component separately, with special consideration to features disturbing the evaluation (regression, halo-like inflammation, etc.). We have concluded that the histological diagnosis of melanocytic tumors is based on the detailed examination of traditional HE slides and the immunohistochemical methods only confirm or weaken our opinion.
Plótár
Vanda
V
Országos Onkológiai Intézet, Daganatpatológiai Osztály, 1122 Budapest Ráth Gy. u. 7-9. plotar.vanda@oncol.hu
Szentirmay
Zoltán
Z
Orosz
Zsolt
Z
D000911:Antibodies, Monoclonal / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D058965:MART-1 Antigen; D008545:Melanoma / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D058950:Melanoma-Specific Antigens; D051739:Microphthalmia-Associated Transcription Factor / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D009506:Nevus / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D018329:Nevus, Blue / Q000737:chemistry; D018332:Nevus, Epithelioid and Spindle Cell / Q000737:chemistry; D018331:Nevus, Spindle Cell / Q000737:chemistry; D016612:Paraffin Embedding; D011133:Polysaccharide-Lyases / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D012878:Skin Neoplasms / Q000737:chemistry* / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D058965:MART-1 Antigen; C497234:MITF protein, human; C548667:MLANA protein, human; D058950:Melanoma-Specific Antigens; D051739:Microphthalmia-Associated Transcription Factor; D009363:Neoplasm Proteins; D009418:S100 Proteins; D011133:Polysaccharide-Lyases; C027349:pectin lyase