[Cloning of PsPIP1 gene from Polygonum sibiricum Laxm. and analy-sis of its expression in response to NaHCO3].
Gene PsPIP1 (GenBank accession No. EU626398) containing a complete ORF was obtained using rapid amplification of cDNA ends (RACE) from the cDNA library of Polygonum sibiricum Laxm. leaves. The length of cDNA was 1 004 bp, which encoded a peptide of 285 amino acid residues. Based on other kinds of plant aquaporin amino acid sequences, the phylogenetic evolution, and tertiary structure of protein comparison, this gene was classified into aquaporin subfamily. Expression analysis by Real-time PCR showed that PsPIP1 gene was expressed in leaves, stems, and underground stems. The expression level of PsPIP1 gene was higher in leaves than in underground stems and was the lowest in stems. The expression pattern of PsPIP1 gene induced by NaHCO3 stress and de-stressing also varied remarkably.
Wang
Yin
Y
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China. wangyin725@hotmail.com
Liu
Guan-Jun
GJ
Yan
Xiu-Feng
XF
Yang
Chuan-Ping
CP
Liu
Ming-Kun
MK
Qu
Chun-Pu
CP
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018547:Plant Stems / Q000235:genetics; D028502:Polygonum / Q000235:genetics*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017693:Sodium Bicarbonate / Q000494:pharmacology*
D018076:DNA, Complementary; D010940:Plant Proteins; D017693:Sodium Bicarbonate