title: Ion mobility-mass spectrometer interface for collisional activation of mobility separated ions.
abstract: An ion mobility-mass spectrometer (IM-MS) interface is described that can be employed to perform collisional activation and/or collision-induced dissociation (CID) with good transmission of mobility separated ions to the MS analyzer. The IM-MS interface consists of a stacked-ring ion guide design, where the field strength and pressure ratio can be operated such that structural rearrangement reactions and/or CID are achieved as a function of the effective ion temperature. The ion dynamics and collisional activation processes in the IM-MS interface are described as a function of the ion-neutral collisions, ion kinetic energies, and effective ion temperature. The applicability of the IM-CID-MS methodology to studies of peptide ion fragmentation is illustrated using a series of model peptides.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Fernandez-Lima
authors.forename: Christopher
authors.initials: C
authors.lastname: Becker
authors.forename: Kent J
authors.initials: KJ
authors.lastname: Gillig
authors.forename: William K
authors.initials: WK
authors.lastname: Russell
authors.forename: Shane E
authors.initials: SE
authors.lastname: Tichy
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
mesh_terms: D000595:Amino Acid Sequence; D007477:Ions / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D007477:Ions; D010455:Peptides