Cloning and transcriptional analysis of groES and groEL in ethanol-producing bacterium Zymomonas mobilis TISTR 548.
Heat and ethanol had an affect not only on growth and cell viability of an obligatorily fermentative Gram-negative bacterium Zymomonas mobilis, but also on protein synthesis. Analysis by SDS-polyacrylamide gel electrophoresis revealed pronounced increasing of two dominant proteins designated as groES and groEL. Molecular cloning of the gene encoding groES and groEL was performed by PCR technique using specific primers synthesized based on the Z. mobilis groESL gene. Sequencing analysis of 2179 bp led to the detection of two open reading frames encoded for 95 and 549 amino acids, respectively. The deduced amino acid sequence of the Z. mobilis groES and groEL shows a high degree of identity with other. The strongly conserved carboxyl-terminus Gly-Gly-Met motif and two small segments, which appear more conserved between ethanol-producing organisms, were found, suggesting that their may be related to stability of protein under heat or ethanol stress. Induction of groES and groEL occurs in response to heat and ethanol, but not to salt stress.
Thanonkeo
Pornthap
P
Department of Biotechnology, Fermentation Research Center for Value Added Agricultural Product, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.
Sootsuwan
Kaewta
K
Leelavacharamas
Vichai
V
Yamada
Mamoru
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D018835:Chaperonin 10 / Q000235:genetics / Q000378:metabolism; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D000431:Ethanol / Q000378:metabolism; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014158:Transcription, Genetic; D017355:Zymomonas / Q000502:physiology*
D018835:Chaperonin 10; D018834:Chaperonin 60; D000431:Ethanol