title: Effect of two different UVA doses on the rabbit cornea and lens.
abstract: The aim of the present paper was to examine the irradiation effect of two doses of UVA rays (365 nm) on the rabbit cornea and lens. Corneas of anesthetized adult albino rabbits were irradiated with UVA rays for 5 days (daily dose 1.01 J cm(-2) in one group of rabbits and daily dose 2.02 J cm(-2) in the second group of animals). The third day after the last irradiation, the rabbits were killed, and their eyes were employed for spectrophotometrical, biochemical and immunohistochemical investigations. Normal eyes served as controls. Absorption spectra of the whole corneal centers were recorded over the UV-VIS (visible) spectral range. Levels of antioxidant and prooxidant enzymes, nitric oxide synthases and nitric oxide (indirectly measured as nitrate concentration) were investigated in the cornea. Malondialdehyde, a byproduct of lipid peroxidation, was examined in the cornea and lens. The results show that the staining for endothelial nitric oxide synthase was more pronounced in corneas irradiated with the higher UVA dose. Otherwise, UVA rays at either dose did not significantly change corneal light absorption properties and did not cause statistically significant metabolic changes in the cornea or lens. In conclusion, UVA rays at the employed doses did not evoke harmful effects in the cornea or lens.
authors.affiliation: Department of Eye Histochemistry and Pharmacology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Cestmír
authors.initials: C
authors.lastname: Cejka
authors.forename: Jan
authors.initials: J
authors.lastname: Pláteník
authors.forename: Richard
authors.initials: R
authors.lastname: Buchal
authors.forename: Vilém
authors.initials: V
authors.lastname: Guryca
authors.forename: Jakub
authors.initials: J
authors.lastname: Sirc
authors.forename: Martin
authors.initials: M
authors.lastname: Vejrazka
authors.forename: Jirina
authors.initials: J
authors.lastname: Crkovská
authors.forename: Taras
authors.initials: T
authors.lastname: Ardan
authors.forename: Jirí
authors.initials: J
authors.lastname: Michálek
authors.forename: Blanka
authors.initials: B
authors.lastname: Brůnová
authors.forename: Jitka
authors.initials: J
authors.lastname: Cejková
mesh_terms: D000818:Animals; D003315:Cornea / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D007908:Lens, Crystalline / Q000528:radiation effects*; D011817:Rabbits; D014466:Ultraviolet Rays