title: Determination of in vivo protein phosphorylation in photosynthetic membranes.
abstract: Light- and redox-controlled reversible phosphorylation of thylakoid proteins regulates short- and long-term acclimation of plants to environmental cues. The major phosphoproteins in thylakoids belong to photosystem II and its light-harvesting antenna but phosphorylation of subunits of other thylakoid protein complexes has been detected as well. The detection methods include electrophoretic separation of proteins and detection of phosphoproteins with a phosphoaminoacid-specific antibody or phosphoprotein-specific dye. The use of mass spectrometry allows the identification of exact phosphorylation site(s) in the proteins. Various methods for detection of phosphoproteins in thylakoids are outlined including phosphopeptide preparation for mass spectrometric analyses and quantitative analysis of protein phosphorylation.
authors.affiliation: Plant Physiology and Molecular Biology, Department of Biology, University of Turku, Turku, Finland.
authors.forename: Julia P
authors.initials: JP
authors.lastname: Vainonen
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Vener
authors.forename: Eva-Mari
authors.initials: EM
authors.lastname: Aro
mesh_terms: D013058:Mass Spectrometry; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D010750:Phosphoproteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins