[Cloning, characterization and application of the promoter region of the alkaline protease gene in Bacillus alcalophillus PB92].
Promoter function fragment of alkaline protease gene (GenBank accession number: EU130686) was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR. Sequenced and analyzed revealed that it contains several typical promoter characterized regions. Two reverse translation frames were located in -538~-370 bp and-275~-128 bp. Deletion analysis of the sequence demonstrated that 414 bp to 619 bp upstream of the TSS showed predominant promoter activity, and a 105 bp length sequence can serve as this function. Additionally, a representative Sec-type signal peptide structure was detected in PB92 AprE signal peptide. By cloning the PaprE and alkaline protease signal peptide gene into pBE2, an expression vector pBEAC was constructed, and a plant sweet protein monellin gene was highly expressed in B. subtilis 1A751.
Chen
Kun
K
Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. chenkun97411@163.com
Li
Ming
M
Cheng
Kun
K
DU
Lian-Xiang
LX
Zhang
Tong-Cun
TC
Lu
Fu-Ping
FP
D000595:Amino Acid Sequence; D001407:Bacillus / Q000145:classification / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics
D001426:Bacterial Proteins; D010940:Plant Proteins; D021382:Protein Sorting Signals; D010450:Endopeptidases; C087822:alkaline protease