title: Phenotypic and genetic diversity of the traditional Lister smallpox vaccine.
abstract: As an initial step in the development of a second-generation smallpox vaccine derived from the Lister strain, to be prepared for a variola virus threat, diversity of the traditional vaccine was examined by characterizing a series of ten viral clones. In vitro and in vivo phenotypic studies showed that the biological behavior of the clones diverged from each other and in most cases diverged from the vaccinia virus (VACV) Lister parental population. Taken together, these results demonstrate the heterogeneity of the viral population within the smallpox vaccine and highlight the difficulty in choosing one clone which would meet the current requirements for a safe and effective vaccine candidate.
authors.affiliation: Laboratoire de Virologie, Centre de Recherches du Service de Santé des Armées Emile Pardé, 24 avenue des Maquis du Grésivaudan, BP87, Grenoble 38702, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Garcel
authors.forename: Julien
authors.initials: J
authors.lastname: Perino
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Crance
authors.forename: Robert
authors.initials: R
authors.lastname: Drillien
authors.forename: Daniel
authors.initials: D
authors.lastname: Garin
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Favier
mesh_terms: D000818:Animals; D001835:Body Weight; D001921:Brain / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D017385:Sequence Homology; D012899:Smallpox / Q000517:prevention & control; D012900:Smallpox Vaccine / Q000235:genetics* / Q000276:immunology*; D016019:Survival Analysis; D014616:Vaccinia virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D012900:Smallpox Vaccine