Amphiphilic triblock copolymers of methoxy-poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) for enhancement of osteoblast attachment and growth.
Amphiphilic triblock copolymers of methoxy-poly(ethylene glycol)-poly(L-lactide)-poly(L-lysine) (MPEG-b-PLLA-b-PLL) (Mn=8540-22 240) were synthesized through the ring-opening polymerization of Nepsilon-(Z)-lysine-N-carboxyanhydrides (N(epsilon)-(Z)-Lys-NCA) using MPEG-b-PLLA-NH2 as a macroinitiator. The triblock copolymers and diblock precursors were characterized by 1H NMR, ATR-FTIR, and GPC. The chain lengths of each block could be controlled by varying the feed ratios of the monomers. The surface properties of films of PLLA modified by blending with the triblock copolymers were investigated by XPS and AFM and demonstrated an enrichment of PLL blocks on the surface of the PLLA film. No cytotoxicity was detected on a range of modified PLLA films arising from the incorporation of the triblock copolymers. The triblock copolymers MPEG-b-PLLA-b-PLL showed better surface properties in promoting osteoblast adhesion and proliferation compared with pure PLLA and PLLA modified with MPEG-b-PLLA diblock copolymers. This study demonstrated that the triblock copolymers containing free amino groups, which self-segregate on the surface of biodegradable polyesters, have potential for applications in cell delivery and tissue engineering.
Peng
Hui
H
Australian Institute for Bioengineering and Nanotechnology, Centre for Magnetic Resonance, University of Queensland, Brisbane QLD 4072, Australia.
Xiao
Yin
Y
Mao
Xueli
X
Chen
Lan
L
Crawford
Ross
R
Whittaker
Andrew K
AK
D002448:Cell Adhesion / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
D046911:Macromolecular Substances; D013501:Surface-Active Agents; C538906:methoxy-poly(ethylene glycol)-b-poly(lactide)-b-poly(lysine); D011107:Polylysine; D011092:Polyethylene Glycols