title: Synthesis of H-bonding probes of alpha7 nAChR agonist selectivity.
abstract: The alpha7 subtype of the nicotinic acetylcholine receptor (nAChR) is the target of studies aimed at identifying features that will lead to the development of selective therapeutics. Five arylidine anabaseines, three with pyridine rings and two with the pyrrole rings, were synthesized in 35-65% yield via aldol condensation. The compounds are homologs of benzylidine anabaseine and were chosen for synthesis because they provide either a hydrogen bond acceptor (pyridines) or hydrogen bond donor (pyrroles) that may interact with the receptor within the benzylidine selectivity motif. Initial analysis of the new compounds at 100 microM concentration reveal that the two pyrrole anabaseines are good partial agonists of the alpha7 nAChR, having 40% of the efficacy of ACh, efficacy comparable to 4OH-GTS-21, and dramatically enhanced efficacy relative to the 2- and 4-pyridinyl compounds. The pyrrole compounds were confirmed to be alpha7 selective, displaying preference for this receptor over muscle and heteromeric neuronal receptor subtypes.
authors.affiliation: Department of Chemistry, University of Florida, PO Box 117200, Gainesville, FL 32611-7200, USA.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Wang
authors.forename: Roger L
authors.initials: RL
authors.lastname: Papke
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Horenstein
mesh_terms: D000818:Animals; D006860:Hydrogen Bonding; D015335:Molecular Probes / Q000138:chemical synthesis*; D018722:Nicotinic Agonists / Q000737:chemistry / Q000494:pharmacology*; D011978:Receptors, Nicotinic / Q000187:drug effects*; D014981:Xenopus; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D015335:Molecular Probes; D018722:Nicotinic Agonists; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor