title: Electrochemical DNA detection based on the polyhedral boron cluster label.
abstract: Polyhedral boron clusters are proposed as new, chemically and biologically stable, versatile redox labels for electrochemical DNA hybridization sensors. Selective and sensitive detection of the redox labeled DNA-probe was achieved by means of covalently attached electroactive marker 7,8-dicarba-nido-undekaborate group. A nanomolar concentration of boron cluster-labeled DNA was recognized. High specificity of the analysis with the boron cluster-labeled DNA probe, including detection of single-base mismatch, was demonstrated. The above findings, together with proposed earlier use of metallacarboranes as an electrochemical label for biomolecules opens the door for a "multicolor" electrochemical coding of DNA with boron clusters and simultaneous detection of several DNA targets.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jelen
authors.forename: Agnieszka B
authors.initials: AB
authors.lastname: Olejniczak
authors.forename: Alena
authors.initials: A
authors.lastname: Kourilova
authors.forename: Zbigniew J
authors.initials: ZJ
authors.lastname: Lesnikowski
authors.forename: Emil
authors.initials: E
authors.lastname: Palecek
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D015374:Biosensing Techniques; D001881:Borates / Q000138:chemical synthesis / Q000737:chemistry*; D001895:Boron / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010084:Oxidation-Reduction; D014529:Uridine / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: C535189:7,8-dicarba-nido-undekaborate; D001881:Borates; D004247:DNA; D001895:Boron; D014529:Uridine