THG-1pit moves to nucleus at the onset of cerebellar granule neurons apoptosis.
Thg-1pit (Tsc22d4), a murine gene belonging to the TGF-beta1-stimulated clone 22 domain (TSC22D) family, is expressed in developing and adult cerebellar granule neurons and mature Purkinje cells. We have studied THG-1pit function in primary cultures of mouse cerebellar granule neurons maintained in vitro in the presence of a medium containing 25 mM K+ (differentiating condition) or 5 mM K+ (pro-apoptotic condition), and determined the effect of culture medium, TGF-beta1 and IGF-1 on THG-1pit expression and intracellular localization. Thg-1pit encoded a 42 kDa MW protein and other, higher MW and developmentally-regulated forms. Cell exposure to 5 mM K+ elicited early and/or late waves of Thg-1pit transcription, depending on the presence/absence of TGF-beta1, and caused THG-1pit to massively and transiently move from cytoplasm and neurites to the nucleus. THG-1pit nuclear entrance was concomitant to that of AIF, suggesting that THG-1pit is involved in the induction of granule neuron apoptosis.
Canterini
Sonia
S
Department of Psychology, Section of Neuroscience, Istituto Pasteur-Fondazione Cenci Bolognetti and "Daniel Bovet" Research Center, La Sapienza University of Rome, Italy.
Bosco
Adriana
A
De Matteis
Valentina
V
Mangia
Franco
F
Fiorenza
Maria Teresa
MT
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D006801:Humans; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
C582288:TSC22D4 protein, human; C443111:Spacdr protein, mouse; D014157:Transcription Factors; D053773:Transforming Growth Factor beta1; D011188:Potassium