Improvement of sensitivity in flow analysis by exploiting a multi-reversed software-assisted system.
A multi-reversed flow system software-assisted was developed for improvement of sensitivity in flow analysis. The performance of the flow system proposed was evaluated by using as a model the conventional Griess' colorimetric reaction for determination of nitrite in waters. The manifold incorporated three 3-way solenoid valves, a relay box solenoid actuated, a peristaltic pump, and a photometric detector. A tailored software was designed and written in Visual Basic 6.0 which allows full control of all flow system components and simultaneous acquisition and processing of the data. The sensitivity measured as the slope of the calibration curve was improved 2.5- and 1.4-fold regarding those obtained by continuous- and stopped-flow systems, respectively. Other valuable features such as analytical throughput of 55 determinations per hour, limit of detection of 5mugL(-1) (3sigma(blank)/slope), relative standard deviation<2% (n=8), and a linear dynamic range up to 1800mugL(-1) were also achieved.
Neira
José Y
JY
Departamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Casilla 237, Concepción, Chile.
González
Elizabeth
E
Nóbrega
Joaquim A
JA