Increased coherence of circadian rhythms in mature fibroblast cultures.
Circadian transcriptional rhythms in confluent NIH 3T3 and PER2::LUCIFERASE fibroblast cultures become more coherent as they mature. This enhanced coherence correlated with increased culture density, while inhibition of the cell cycle increased coherence in immature cultures. Mature cultures may therefore express more coherent rhythms for longer because of their reduced rate of cell division. The authors conclude that mature fibroblast cultures will be of great utility for high-throughput pharmacological and mutational circadian screening.
O'Neill
John S
JS
Centre for Systems Biology at Edinburgh, Mayfield Road, Edinburgh, UK. john.oneill@ed.ac.uk
Hastings
Michael H
MH
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D018797:Cell Cycle Proteins / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000235:genetics; D019384:Nucleic Acid Synthesis Inhibitors; D056950:Period Circadian Proteins; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection
D018797:Cell Cycle Proteins; D004791:Enzyme Inhibitors; D009687:Nuclear Proteins; D019384:Nucleic Acid Synthesis Inhibitors; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D014157:Transcription Factors; D016590:Aphidicolin; D008156:Luciferases