title: Detection of an antibody to avian influenza virus by an electrochemical immunoassay (eELISA).
abstract: The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10 - 1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.
authors.affiliation: Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu 804-8550, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Endo
authors.forename: Kimio
authors.initials: K
authors.lastname: Morimoto
authors.forename: Bui N
authors.initials: BN
authors.lastname: Vuong
authors.forename: Haruko
authors.initials: H
authors.lastname: Ogawa
authors.forename: Kunitoshi
authors.initials: K
authors.lastname: Imai
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D004209:Disposable Equipment; D004563:Electrochemistry; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005240:Feasibility Studies; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D000914:Antibodies, Viral; D009943:Organophosphorus Compounds; C068798:4-aminophenylphosphate; D000469:Alkaline Phosphatase