Comparative proteomic analysis of rat juvenile and adult dura.
BACKGROUND
It has been widely observed that infants and young children can reossify large calvarial defects when they are younger than 2 years of age; afterwards, they lose this regenerative potential. Previous studies have implicated that the dura mater serves as a key regulator of calvarial regeneration. However, the molecular mechanism of calvarial reossification remains elusive.


METHODS
In order to identify the proteins that may participate in this process, we performed a proteome-wide comparison of the protein expression levels of immature and mature dura using 2D electrophoresis and MALDI-TOF mass spectrometry. The Western blotting was used to verify the results of the 2D electrophoresis/MALDI-TOF mass spectrometry.


RESULTS
Eleven proteins were found to express with significant differences in the immature and the mature dura. Among them, the emergence of vimentin, tropomyosin, beta-actin and gamma-actin were further confirmed by the Western blotting analysis.


CONCLUSIONS
The proteins and proteomic profiles provide a better understanding of the molecular mechanism of calvarial regeneration.
Song
Tao
T
Department of Craniomaxillofacial Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Bi
Nan
N
Peng
Zhe
Z
Gui
Lai
L
Chen
Bao-sheng
BS
D000375:Aging / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D004388:Dura Mater / Q000737:chemistry*; D004586:Electrophoresis; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley
D011506:Proteins