title: A cathepsin L-like protease from Strongylus vulgaris: an orthologue of Caenorhabditis elegans CPL-1.
abstract: Cathespin L-like proteases (CPLs), characterized from a wide range of helminths, are significant in helminth biology. For example, in Caenorhabditis elegans CPL is essential for embryogenesis. Here, we report a cathepsin L-like gene from three species of strongyles that parasitize the horse, and describe the isolation of a cpl gene (Sv-cpl-1) from Strongylus vulgaris, the first such from equine strongyles. It encodes a protein of 354 amino acids with high similarity to other parasitic Strongylida (90-91%), and C.elegans CPL-1 (87%), a member of the same Clade. As S.vulgaris cpl-1 rescued the embryonic lethal phenotype of the C.elegans cpl-1 mutant, these genes may be orthologues, sharing the same function in each species. Targeting Sv-CPL-1 might enable novel control strategies by decreasing parasite development and transmission.
authors.affiliation: School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Sinéad Nic An
authors.initials: SN
authors.lastname: Ultaigh
authors.forename: James C
authors.initials: JC
authors.lastname: Carolan
authors.forename: Collette
authors.initials: C
authors.lastname: Britton
authors.forename: Linda
authors.initials: L
authors.lastname: Murray
authors.forename: Michael F
authors.initials: MF
authors.lastname: Ryan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics*; D056668:Cathepsin L; D002403:Cathepsins / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D006736:Horses; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D018510:RNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D013323:Strongylus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth; D002403:Cathepsins; C466023:CPL-1 protein, C elegans; D056668:Cathepsin L