[Overexpression of PDZ1 domain of PSD-95 protein rescues hippocampal neurons from apoptosis induced by oxygen-glucose deprivation].
To detect the effect of PDZ1, domain of postsynaptic density 95 (PSD-95), on apoptosis of hippocampal neurons induced by oxygen-glucose deprivation (OGD), Sprague-Dawley rat hippocampal neurons were infected with PDZ1-viruses after 21 days of plating. Twenty-four hours after infection, cells were treated with OGD for 1.5 h, then were incubated with DAPI and apoptosis-like cells were characterized, or were collected for co-immunoprecipitation and Western blot analyses. The results showed that: (1) PDZ1 overexpression was observed in hippocampal neurons; (2) Apoptosis induced by OGD was obviously decreased in neurons overexpressing PDZ1 (P<0.05); (3) Overexpression of PDZ1 prevented the binding of GluR6 to PSD-95; (4) Overexpression of PDZ1 inhibited MLK3 and JNK1/2 activation induced by OGD. These results indicate that overexpression of PDZ1 may prevent hippocampal neurons from apoptosis induced by OGD.
Hu
Shu-Qun
SQ
Xuzhou Medical College, Xuzhou, China.
Zong
Yan-Yan
YY
Zhang
Guang-Yi
GY
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D000076122:Disks Large Homolog 4 Protein; D005947:Glucose / Q000737:chemistry; D006624:Hippocampus / Q000166:cytology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000473:pathology; D010100:Oxygen / Q000737:chemistry; D054731:PDZ Domains; D051381:Rats; D017207:Rats, Sprague-Dawley
D003470:Culture Media; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D005947:Glucose; D010100:Oxygen