A rapid HPLC method for the quantification of 3,5,4'-trimethoxy-trans-stilbene (TMS) in rat plasma and its application in pharmacokinetic study.
A rapid HPLC-UV method had been developed and validated to quantify 3,5,4'-trimethoxy-trans-stilbene (TMS), a naturally occurring and pharmacologically active analog of resveratrol in rat plasma. The samples were mixed with three volumes of acetonitrile to precipitate protein. Chromatographic separation was achieved on a RP-HPLC column (Agilent ZORBAX Eclipse Plus C18: 250 mm x 4.6mm i.d., 5microm), which was protected by a guard column (Agilent ZORBAX Eclipse Plus C18: 12.5 mm x 4.6mm i.d., 5microm) through isocratic delivery of a mobile phase of acetonitrile: water (75:25, v/v) at a flow rate of 1.2ml/min. The assay was executed at 30 degrees C and the UV absorbance at 320nm was monitored. The retention time of TMS and trans-stilbene (internal standard) was 6.5 and 8.3min, respectively. The calibration curve was linear within the range of 15-1000ng/ml (R(2)>0.998) and 15ng/ml was the lower LOQ. The intra- and inter-day precisions were good and the RSD was all lower than 7.3%. The mean absolute recovery of TMS in plasma ranged from 99.2 to 104.1%. This HPLC method had been successfully applied to study the pharmacokinetics of TMS, which was fully dissolved with hydroxypropyl-beta-cyclodextrin (HP-beta-CyD). In comparison with resveratrol, TMS had greater plasma exposure, longer elimination half-life and lower clearance. As TMS had superior pharmacokinetic characteristics, its potential as a preventive or therapeutic agent in resveratrol-effective conditions or diseases should be considered.
Lin
Hai-Shu
HS
Department of Pharmacy, National University of Singapore, Singapore 119260, Singapore. phalh@nus.edu.sg
Ho
Paul C
PC
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D017408:Guidelines as Topic; D006207:Half-Life; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D000077185:Resveratrol; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013267:Stilbenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D012997:Solvents; D013267:Stilbenes; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000077185:Resveratrol