Live single-cell metabolomics of tryptophan and histidine metabolites in a rat basophil leukemia cell.
A direct and rapid metabolic analysis of a live single cell was performed by live single-cell video-mass spectrometry. The contents of the cytoplasm and a granule were sucked into a nano-electrospray ionization (nano-ESI) tip, and were directly introduced into a Q-TOF mass spectrometer by nano-spray after the addition of an ionization solvent. The metabolic pathways and the locations of tryptophan and histidine metabolites were traced by this method for a cultured rat basophil leukemia cell line (RBL-2H3). The t-values of detected peaks by a t-test between the different location, e.g. cytoplasm and a granule, revealed the molecular localization of each MS peak. A direct and quick metabolomic analysis of a living cell under simultaneous video-microscopic observations was innovated.
Mizuno
Hajime
H
Graduate School of Biomedical Sciences, Hiroshima University, Japan.
Tsuyama
Naohiro
N
Date
Sachiko
S
Harada
Takanori
T
Masujima
Tsutomu
T
D000818:Animals; D001491:Basophils / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D006639:Histidine / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology*; D055432:Metabolomics; D036103:Nanotechnology; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014364:Tryptophan / Q000378:metabolism*
D006639:Histidine; D014364:Tryptophan