An assessment of Wx microsatellite allele, alkali degradation and differentiation of chloroplast DNA in traditional black rice (Oryza sativa L.) from Thailand and Lao PDR.
Thailand and Lao PDR are the country's rich rice diversity. To contribute a significant knowledge for development new rice varieties, a collection of 142 black rice (Oryza sativa) accessions were determined for variation of physico-chemical properties, Wx microsatellite allele, Wx allele and chloroplast DNA type. The results showed that amylose content of black rice accessions were ranged from 1.9 to 6.8%. All of the alkali disintegration types (high, intermediate and low) was observed in these rice with average of 1.75 on the 1-3 digestibility scale. The unique Wx microsatellite allele (CT)17 was found in these samples and all black rice strains carried Wx(b) allele. In addition, all black rice accessions were found the duplication of the 23 bp sequence motif in the exon 2 of the wx gene. This evidence is a common phenomenon in glutinous rice. Based on two growing condition for black rice, rainfed lowland and rainfed upland, chloroplast DNA type was distinct from each other. All rice strains from rainfed lowland was deletion plastotype, but all other rainfed upland strains were non-deletion types.
Prathepha
Preecha
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Walai Rukhavej Botanical Research Institute, Mahasarakham University, Khamriang District, Mahasarakham Province, 44150, Thailand.
D000688:Amylose / Q000032:analysis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018742:DNA, Chloroplast / Q000235:genetics*; D014644:Genetic Variation; D007812:Laos; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013214:Starch Synthase / Q000235:genetics*; D013785:Thailand
D017931:DNA Primers; D018742:DNA, Chloroplast; D000688:Amylose; D013214:Starch Synthase