6,7-Dimethyllumazine as a potential ligand for selective recognition of adenine opposite an abasic site in DNA duplexes.
6,7-Dimethyllumazine more selectively binds to adenine (A) base opposite the abasic site in DNA duplexes (5'-TCC AGX[combining low line] GCA AC-3'/3'-AGG TCN CGT TG-5', X = AP site (Spacer C3), N= A, T, C and G) than the other three nucleobases with a dissociation constant K(d) of ca. 1.0 microM; substituted methyl groups enhance the binding affinity to A and the selectivity for A over T, compared to the parent molecule, lumazine.
Ye
Zhiqiang
Z
Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
Rajendar
Burki
B
Qing
Dai
D
Nishizawa
Seiichi
S
Teramae
Norio
N
D000225:Adenine / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D011621:Pteridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
C534637:6,7-dimethyllumazine; D008024:Ligands; D011621:Pteridines; D004247:DNA; D000225:Adenine