Her4 is necessary for establishing peripheral projections of the trigeminal ganglia in zebrafish.
Transcripts of notch and its target genes have been detected in some differentiating neurons. However, the role of Notch in neuronal differentiation remains poorly defined. Here, we show that a subset of differentiating sensory neurons in the trigeminal ganglia express her4. Expression of her4 requires Notch signaling during neurogenesis but not during differentiation, when peripheral projections of the trigeminal ganglia are established. These projections develop poorly in her4 morphants. While many components of the canonical Notch signaling pathway are not required for late her4 expression or peripheral axon outgrowth in trigeminal neurons, simultaneous knock-down of Notch receptors prevents establishment of these peripheral projections. These observations suggest that Her4 and Notch play a role in peripheral outgrowth of sensory neurons.
So
Ju-Hoon
JH
Department of Biology, Chungnam National University, Daejeon, Republic of Korea.
Chun
Hang-Suk
HS
Bae
Yong-Ki
YK
Kim
Hyun-Seo
HS
Park
Yeoll-Mae
YM
Huh
Tae-Lin
TL
Chitnis
Ajay B
AB
Kim
Cheol-Hee
CH
Yeo
Sang-Yeob
SY
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism; D014158:Transcription, Genetic; D012668:Trigeminal Ganglion / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch; D029961:Zebrafish Proteins; C119433:her4.1 protein, zebrafish; C486337:neurog1 protein, zebrafish