title: Multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel.
abstract: We have developed a system to detect three hydrolytic enzymes (cellulase, lipase, and protease) using a single sodium dodecyl sulfate (SDS) gel and an electrotransfer system. After electrophoresis, proteins in the gel were transferred to three sandwiched substrate gels containing glycerol tributyrate, azo-carboxymethyl cellulose (Azo-CMC), and fibrin for detection of cellulase, lipase, and protease, respectively. We show that three cellulases (from a Paenibacillus sp. and two Bacillus sp. strains), one lipase (from a Staphylococcus sp.), and two proteases (from two Bacillus sp. strains) can be detected simultaneously with our zymogram system.
authors.affiliation: Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology, Sinjeong-dong, Jeonbuk, Republic of Korea.
authors.forename: Nack-Shick
authors.initials: NS
authors.lastname: Choi
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Kim
authors.forename: Chang-Su
authors.initials: CS
authors.lastname: Park
authors.forename: Yun Jon
authors.initials: YJ
authors.lastname: Han
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Choi
authors.forename: Seung-Goo
authors.initials: SG
authors.lastname: Lee
authors.forename: Jae Jun
authors.initials: JJ
authors.lastname: Song
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D002480:Cellulase; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004798:Enzymes / Q000302:isolation & purification*; D008049:Lipase; D008722:Methods; D010447:Peptide Hydrolases; D012107:Research Design
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D008049:Lipase; D002480:Cellulase; D010447:Peptide Hydrolases