Combination of different molecular mechanisms leading to fluconazole resistance in a Candida lusitaniae clinical isolate.
We report on the underlying molecular mechanisms likely responsible for the high-level fluconazole resistance in a Candida lusitaniae clinical isolate. Fluconazole resistance correlated with overexpression of ERG11 and of several efflux pump genes, in particular, the orthologs of the Candida albicans MDR1, PDR16, CDR1, CDR2, and YOR1.
Reboutier
David
D
Programme Chimiorésistance des Levures Pathogènes, EA209, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 75006 Paris, France.
Piednoël
Mathieu
M
Boisnard
Stéphanie
S
Conti
Audrey
A
Chevalier
Virginie
V
Florent
Martine
M
Gibot-Leclerc
Stéphanie
S
Da Silva
Bruno
B
Chastin
Christiane
C
Fallague
Karim
K
Favel
Anne
A
Noël
Thierry
T
Ruprich-Robert
Gwenaël
G
Chapeland-Leclerc
Florence
F
Papon
Nicolas
N
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D002175:Candida / Q000187:drug effects* / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D017931:DNA Primers; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D015725:Fluconazole; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D008780:Methyltransferases