title: The SLC6/NSS family members KAAT1 and CAATCH1 have weak chloride dependence.
abstract: KAAT1 and CAATCH1 are amino acid transporters cloned from the intestine of the lepidoptera Manduca sexta. They are members of the SLC6/NSS family, which groups membrane proteins that use Na(+), K(+), and Cl⁻ gradients for the coupled transport of amines and amino acids. The report of the atomic-resolution x-ray crystal structure of the eubacterium Aquifex aeolicus leucine transporter (AaLeuT) has contributed significantly to understanding of the structure-function relationship in NSS proteins. Transport by AaLeuT is Cl⁻ independent, whereas many neurotransmitter:sodium symporters like serotonin transporter (SERT), GABA transporter (GAT1), dopamine transporter, and norephinephrine transporter, among others, are strongly Cl⁻ dependent.
authors.affiliation: Institute of General Physiology and Biological Chemistry G. Esposito, School of Pharmacy, University of Milan, Milano, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Bettè
authors.forename: Michela
authors.initials: M
authors.lastname: Castagna
authors.forename: Elena
authors.initials: E
authors.lastname: Bossi
authors.forename: Antonio
authors.initials: A
authors.lastname: Peres
authors.forename: Vellea Franca
authors.initials: VF
authors.lastname: Sacchi
mesh_terms: D000588:Amines / Q000378:metabolism; D026921:Amino Acid Transport Systems, Neutral / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002712:Chlorides / Q000737:chemistry*; D050485:GABA Plasma Membrane Transport Proteins / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism*; D018402:Manduca / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism
chemical_list: D000588:Amines; D026921:Amino Acid Transport Systems, Neutral; D000596:Amino Acids; C413023:CAATCH1 protein, insect; D002352:Carrier Proteins; D002712:Chlorides; D050485:GABA Plasma Membrane Transport Proteins; D019476:Insect Proteins; C112249:KAAT1 protein, insect; D008565:Membrane Proteins; D018377:Neurotransmitter Agents