title: Allelic variation of calsyntenin 2 (CLSTN2) modulates the impact of developmental tobacco smoke exposure on mnemonic processing in adolescents.
abstract: BACKGROUND
  Exposure to nicotine in tobacco smoke during development has been linked to subsequent deficits in attention and memory. The present study tested for evidence that genetic variation may contribute to individual differences in vulnerability to the effects of developmental exposure to tobacco smoke on memory and medial temporal lobe function in adolescents.
  
  
  METHODS
  Verbal and visuospatial memory were assessed and functional magnetic resonance imaging (fMRI) data were acquired in 101 adolescents systematically characterized for prenatal and adolescent exposure to tobacco smoke, while they performed an encoding and recognition memory task. The impact of allelic variation at loci within CLSTN2 (encoding synaptic protein calsyntenin 2) and KIBRA, shown previously to modulate early and delayed recall of words, on the dependent measures was examined.
  
  
  RESULTS
  KIBRA genotype did not exert significant main or interacting effects with prenatal or adolescent exposure to tobacco smoke on verbal or visuospatial memory. Previous observations of a beneficial effect of the CLSTN2 C allele on verbal recall were replicated. Adolescent exposure to tobacco smoke reversed this beneficial effect and was associated with increased activation of parahippocampal gyrus during early and delayed recognition in CLTSN2 C allele carriers. While the CLSTN2 C allele conferred enhanced functional connectivity between brain regions subserving accurate verbal recognition, adolescent exposure to tobacco smoke reversed this effect.
  
  
  CONCLUSIONS
  These findings extend previous work demonstrating that calsyntenins play an essential role in learning and indicate that this role is modulated both by CLSTN2 genotype and, during adolescent development, by exposure to tobacco smoke.
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut 06320, USA. leslie.jacobsen@pfizer.com
authors.forename: Leslie K
authors.initials: LK
authors.lastname: Jacobsen
authors.forename: Marina R
authors.initials: MR
authors.lastname: Picciotto
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Heath
authors.forename: W Einar
authors.initials: WE
authors.lastname: Mencl
authors.forename: Joel
authors.initials: J
authors.lastname: Gelernter
mesh_terms: D000293:Adolescent; D000294:Adolescent Behavior / Q000502:physiology*; D041923:Adolescent Development / Q000502:physiology; D022981:Allelic Imbalance; D001921:Brain / Q000503:physiopathology; D002135:Calcium-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008569:Memory Disorders / Q000209:etiology* / Q000235:genetics* / Q000503:physiopathology; D010750:Phosphoproteins; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D013702:Temporal Lobe / Q000503:physiopathology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: C472551:CLSTN2 protein, human; D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011506:Proteins; D014028:Tobacco Smoke Pollution; C513637:WWC1 protein, human