Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo Grande, MS, Brazil.
We analyzed fecal samples from hospitalized children up to three years of age with acute gastroenteritis at Campo Grande, Mato Grosso do Sul, Brazil, from May 2000-January 2004. Astrovirus and calicivirus were detected by Reverse Transcription-Polymerase Chain Reaction and adenovirus was detected using the Rotavirus and Adenovirus combined immunoenzyme assay. Astrovirus, adenovirus and calicivirus were detected at rates of 3.1%, 3.6% and 7.6%, respectively. These results re-emphasize the need for the establishment of regional vigilance systems to evaluate the impact of enteric viruses on viral gastroenteritis.
Andreasi
Marcia Sueli Assis
MS
Departamento de Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil. msaa@nin.ufms.br
Cardoso
Divina das Dores de Paula
Dd
Fernandes
Sonia Maria
SM
Tozetti
Ines Aparecida
IA
Borges
Ana Maria Tavares
AM
Fiaccadori
Fabíola Souza
FS
Santos
Rodrigo Alessandro Tôgo
RA
Souza
Menira
M
D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000453:epidemiology*; D000260:Adenoviruses, Human / Q000302:isolation & purification; D019350:Astroviridae Infections / Q000175:diagnosis / Q000453:epidemiology*; D001938:Brazil / Q000453:epidemiology; D002139:Caliciviridae / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology*; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D001257:Mamastrovirus / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction