title: CMV enhancer-promoter is preferentially active in exocrine cells in vivo.
abstract: The CMV enhancer-promoter sequence is often used as a transcriptional regulatory element in vector systems. We have used this control element to drive expression of GFP in a lentivirus vector transgene in pigs and chickens. Promoted as a 'universal' enhancer/promoter element capable of transcriptional activity in a number of cells in vitro, CMV-GFP transgene expression in vivo is preferentially observed in exocrine cells. This expression profile validates the use of this transcriptional control sequence to target expression to exocrine cells in gene transfer strategies.
authors.affiliation: Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, EH25 9PS, Scotland, UK. douglas.vasey@bbsrc.ac.uk
authors.forename: D B
authors.initials: DB
authors.lastname: Vasey
authors.forename: S G
authors.initials: SG
authors.lastname: Lillico
authors.forename: H M
authors.initials: HM
authors.lastname: Sang
authors.forename: T J
authors.initials: TJ
authors.lastname: King
authors.forename: C B A
authors.initials: CB
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens; D003587:Cytomegalovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013552:Swine; D014158:Transcription, Genetic; D019076:Transgenes
chemical_list: D016328:NF-kappa B; D049452:Green Fluorescent Proteins