title: NdgR, an IclR-like regulator involved in amino-acid-dependent growth, quorum sensing, and antibiotic production in Streptomyces coelicolor.
abstract: NdgR (regulator for nitrogen source-dependent growth and antibiotic production), an IclR-like regulator, has been initially identified as a binding protein to the promoters of doxorubicin biosynthetic genes in Streptomyces peucetius by DNA affinity capture assay method. NdgR is well conserved throughout the Streptomyces species and many other bacteria such as Mycobacteria and Corynebacteria. In Streptomyces coelicolor, ndgR deletion mutant showed slow cell growth and defects in differentiation and enhances the production of actinorhodin (ACT) in minimal media containing certain amino acids where wild-type strain could not produce ACT. Although deletion mutant of ndgR showed different antibiotic production in minimal media containing Leu or Gln, it only showed reduced mRNA expression levels of the genes involved in leucine metabolism. Neither NdgR-dependent expression of glnA nor direct binding of NdgR protein to glnA, glnII, and glnR promoters was observed. However, ScbR, which is governed by NdgR shown by gel mobility shift assay, binds to promoter of glnR, suggesting indirect regulation of glutamine metabolism by NdgR. NdgR protein binds to intergenic region of ndgR-leuC, and scbR-scbA involved in gamma-butyrolactone. Two-dimensional gel analysis has shown a global effect of ndgR deletion in protein expression, including up-regulated proteins involved in ACT synthesis and down-regulation of chaperones such as GroEL, GroES, and DnaK. These results suggest a global regulatory role for NdgR in amino acid metabolisms, quorum sensing, morphological changes, antibiotic production, and expression of chaperonines in S. coelicolor.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
authors.forename: Eunjung
authors.initials: E
authors.lastname: Song
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Kim
authors.forename: Kwangwon
authors.initials: K
authors.lastname: Lee
authors.forename: Woo-Seong
authors.initials: WS
authors.lastname: Kim
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Park
authors.forename: Ji-Sook
authors.initials: JS
authors.lastname: Hahn
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D053038:Quorum Sensing; D016415:Sequence Alignment; D048372:Streptomyces coelicolor / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D000095622:Benzoisochromanequinones
chemical_list: D000596:Amino Acids; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C013390:actinorhodin; D000095622:Benzoisochromanequinones