The effect of sample storage on the reliability of thyroglobulin and thyroglobulin-antibody measurements.
OBJECTIVE
To investigate how sample-storage alters thyroglobulin (Tg) and thyroglobulin-antibody (TgAb) immunoreactivity.


METHODS
Tg and TgAb measurements at different storage-times and -temperatures.


RESULTS
48-h-storage at 4-10 degrees C (but not 8 h at room temperature) increased Tg and decreased TgAb immunoreactivity by maximum +23% and -16%. 4-weeks-storage at -17 to -20 degrees C decreased both Tg and TgAb by max. 21-31%.


CONCLUSIONS
Storage of samples at 4-10 degrees C for >24-48 h, or at -17 to -20 degrees C for >2-3 weeks significantly alters Tg and TgAb immunoreactivity.
Locsei
Zoltán
Z
1st Department of Medicine, Markusovszky Teaching Hospital of County Vas, Szombathely, Hungary.
Toldy
Erzsébet
E
Szabolcs
István
I
Rácz
Károly
K
Kovács
Gábor L
GL
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000592:standards*; D013696:Temperature; D013954:Thyroglobulin / Q000032:analysis*; D013997:Time Factors
D001323:Autoantibodies; C006606:anti-thyroglobulin; D013954:Thyroglobulin