[Association of -6735T-->C variant of glucokinase-associated dual-specificity phosphatase 12 gene with type 2 diabetes in Chinese].
OBJECTIVE
To investigate the impact of glucokinase-associated dual-specificity phosphatase 12 gene (DUSP12) single nucleotide polymorphism (SNP) on type 2 diabetes mellitus in Chinese.


METHODS
The genotypes of -6735T-->C of DUSP12 were determined by PCR-RFLP in 577 Chinese in Shanghai, 359 with normal glucose tolerance (NGT) and 218 being newly diagnosed DM patients without taking any drug. Oral glucose tolerance test was conducted. Height, weight, glucose and insulin concentrations, serum lipid profiles, and fat distribution parameters were determined.


RESULTS
The genotype frequency distributions of -6735T-->C variant in DUSP12 gene of the DM group and NGT group were not significantly different (all P > 0.05). The fasting plasma glucose concentration of the NGT subjects with C allele was 5.00 (4.69 - 5.28) mmol/L, significantly higher than that of the subjects with TT genotype [4.90 (4.50 - 5.26) mmol/L, P = 0.035]. The serum HDL-c concentration of the NGT subjects with C allele was (1.25 +/- 0.30) mmol/L, significantly lower than that of those with TT genotype [(1.34 +/- 0.30) mmol/L, P = 0.010]. These two results still showed significant statistical differences after adjusted with sex, age, and BMI (P = 0.035).


CONCLUSIONS
-6735T-->C variant in DUSP12 doesn't play a major role in diabetes, but it may have some effects on glucose and lipid metabolism.
Gao
Fei
F
Shanghai Clinical Medicine Center for Diabetes, Shanghai Diabetes Institute, Department of Endocrinology and Metabolism, Shanghai Jiaotong University Affiliated No.6 People's Hospital, Shanghai 200233, China.
Zhang
Rong
R
Hu
Cheng
C
Fang
Qi-chen
QC
Wang
Cong-rong
CR
Ma
Xiao-jing
XJ
Lu
Jun-xi
JX
Xu
Jing
J
Jia
Wei-ping
WP
Xiang
Kun-san
KS
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D054637:Dual-Specificity Phosphatases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005941:Glucokinase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
D005941:Glucokinase; C489855:dual specificity phosphatase 12; D054637:Dual-Specificity Phosphatases