Shoot micropropagation and microtuberization in potato (Solanum tuberosum L.) by the semi-continuous bioreactor.
The aim of this investigation is introducing the use of continuous and semi-continuous bioreactors and their functions at shoot multiplication and microtuberization of potato. The study shows that the explants have several nodes and when they are suspended continuously under the liquid culture medium, the shoot micropropagations and microtuberization was inhibited. The surfaces of the explants were formed callus and subsequently, they were died. However, in the semicontinuous bioreactor, with the periodical pumping of the nutritional medium the explants aren't continuously suspended under the nutritional solution. The shoot micropropagation, the leaf growth and, the root formation are suitable. In the microtuber inductive medium, the numerous of the tubers are induced. They were sessile tubers. The dormancy of the tubers are long-term, spourting after 3-4 months in the room conditions.
Ebadi
Mostafa
M
Azad Islamic University-Damghan Branch, Damghan, Iran.
Iranbakhsh
Alireza
A
Khaniki
Gholamreza Bakhshi
GB
D019149:Bioreactors; D046508:Culture Techniques; D018520:Plant Shoots / Q000254:growth & development*; D035281:Plant Tubers / Q000254:growth & development*; D011198:Solanum tuberosum / Q000254:growth & development*