title: [Application of liquid chromatography in substitution of the radioimmunoassay technique in order to reduce residues generated in health services in research laboratory].
abstract: Designing a Health Care Service Waste Management Plan, according to the RDC 306 rules, is a responsibility of all those who produce such waste. Since radioimmunoassay (RIA) is one of the most employed techniques, we studied the impact of replacing this technique by liquid chromatography (HPLC) with regard to the reduction of the radioactive residues routinely produced by the Unifesp steroid laboratory. The residues produced by the determination of serum cortisol and 17 alpha-hydroxyprogesterone were classified, and those belonging to groups B and C were evaluated. We observed that, when RIA is used, chemical residues (group B) and radioactive waste (group C) are produced, whereas HPLC generates only chemical residues. Adequation of these techniques showed to be advantageous, by significantly reducing the time of analysis and mainly by eliminating and/or reducing the generation of radioactive waste, encouraging its application to other methodologies, as well as its adoption by other research units.
authors.affiliation: Laboratório de Esteróides, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Luciane M
authors.initials: LM
authors.lastname: Ribeiro Neto
authors.forename: Eduardo K
authors.initials: EK
authors.lastname: Sugawara
authors.forename: Ieda T N
authors.initials: IT
authors.lastname: Verreschi
mesh_terms: D019326:17-alpha-Hydroxyprogesterone / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D006298:Health Services Administration / Q000592:standards*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007753:Laboratories; D017744:Medical Waste Disposal; D011635:Public Health Administration; D011863:Radioimmunoassay; D012037:Refuse Disposal / Q000592:standards; D018505:Waste Management / Q000592:standards*; D014874:Water Pollutants, Chemical
chemical_list: D017744:Medical Waste Disposal; D014874:Water Pollutants, Chemical; D019326:17-alpha-Hydroxyprogesterone; D006854:Hydrocortisone