Alpha/beta hydrolase 1 is upregulated in D5 dopamine receptor knockout mice and reduces O2- production of NADPH oxidase.
Renal dopamine receptors have been shown to play a critical role in ROS-dependent hypertension. D5 dopamine receptor deficient (D5-/-) mice are hypertensive and have increased systemic oxidative stress which is manifested in the kidney and the brain. To further investigate the underlying mechanisms of hypertension in D5-/- mice, we used RNA arrays to compare mRNA levels of kidneys from wildtype and D5-/- mice. Our data show, that the mRNA level of alpha/beta hydrolase 1 (ABHD1) is significantly upregulated in D5-/- mice. Additionally, overexpression of ABHD1 in a new established renal proximal tubule cell line reduced the amount of O(2)(-) produced by the NADPH oxidase. Therefore the upregulation of ABHD1 in D5-/- mice could be an answer to the increased oxidative stress. While oxidative stress is an important factor for the development of hypertension, ABHD1 could play a protective role in the pathogenesis of hypertension.
Stoelting
Miriam
M
Medical Clinic and Policlinic D, University clinics Münster, Münster, Germany.
Geyer
Marcel
M
Reuter
Stefan
S
Reichelt
Rudolf
R
Bek
Martin Johannes
MJ
Pavenstädt
Hermann
H
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D006867:Hydrolases / Q000235:genetics*; D006977:Hypertension, Renal / Q000235:genetics* / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D018345:Mice, Knockout; D019255:NADPH Oxidases / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D010100:Oxygen / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism; D050641:Receptors, Dopamine D5 / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D050641:Receptors, Dopamine D5; D019255:NADPH Oxidases; D006867:Hydrolases; C454239:LABH1, mouse; D010100:Oxygen