[Variation of MSTN gene UTR in eleven sheep breeds].
PCR-RFLP was applied to analyze the polymorphism of MSTN gene UTR in 345 sheep that comprised of eleven sheep breeds, namely Texel sheep, Charolais sheep, Small-tailed Han sheep, Monggolian sheep, Ujumqin sheep, Altay Fat-rumped sheep, Hulunbeir sheep, Tashikurgan sheep, Duolang sheep, Hu sheep, and Gangba sheep. A 271 bp and a 1 003 bp long PCR products were digested with Mboand Bsato demonstrate polymorphism in the eleven sheep breeds, which were all at Hardy-Weinberg equilibrium (P>0.05). The distribution of 3 genotypes in 11 sheep breeds was significantly different (P<0.01). Digestion of the PCR products with HpyCH4 proved that 9 domestic local sheep breeds were different from Texel sheep in the SNP site that was associated with muscularity. The individual mutation base could generate the motifs for miRNA in the 3'UTR, and sequencing analysis demonstrated high frequency of mutation in the 3'UTR region.
Meng
Xiang-Ren
XR
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. mxryx@163.com
Guo
Jun
J
Zhao
Qian-Jun
QJ
Ma
Yue-Hui
YH
Guan
Wei-Jun
WJ
Liu
Di
D
Di
Ran
R
Qiao
Hai-Yun
HY
Na
Ri-Su
RS
D000818:Animals; D001947:Breeding; D014644:Genetic Variation; D009154:Mutation; D055435:Myostatin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012756:Sheep / Q000235:genetics*; D020506:Untranslated Regions / Q000235:genetics*
D055435:Myostatin; D020506:Untranslated Regions