[Membrane-initiated nongenomic effect on mitogen-activated protein kinase signal transduction of estrogen in endometrial carcinoma cell].
OBJECTIVE
To study the nongenomic effect on mitogen-activated kinase (MAPK) signal transduction of 17beta-estradiol at plasma membrane in endometrial cancer cell Ishikawa.


METHODS
Estradiol-bovine serum albumin conjugates (E(2)-BSA) was added to Ishikawa cells at different time points. Western blot was used to detect the protein expression of phosphorylated extracellular signal-regulated kinase (ERK) 1/2. Confocal was used to verify if the phosphorylation of ERK1/2 was initiated at the membrane.


RESULTS
Phosphorylation of ERK1/2 was elicited after 5 minute exposure to 1 x 10(-7) mol/L E(2)-BSA. It was induced to a maximum following 60 minute exposure and descended after 2 hours. The expression levels of phosphorylated ERK1/2 were 0.52, 0.98, and 0.09, respectively. Confocal proved that E(2)-BSA combined with membrane receptors after 5 minutes and elevated the phosphorylation of ERK1/2.


CONCLUSIONS
Membrane actions of estrogen can activate the rapid signaling cascades of MAPK in endometrial carcinoma cells.
Zhang
Li-Li
LL
Department of Gynecology, Peking University People's Hospital, Beijing 100044, China.
Li
Xiao-Ping
XP
Wang
Jian-Liu
JL
Wei
Li-Hui
LH
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D004789:Enzyme Activation; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
D011956:Receptors, Cell Surface; C043090:estradiol-bovine serum albumin; D012710:Serum Albumin, Bovine; D004958:Estradiol; D019950:Mitogen-Activated Protein Kinase 1; D020928:Mitogen-Activated Protein Kinases