Real-time polymerase chain reaction detection methods.
Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification. Real-time assay chemistries are subdivided into several main categories including DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer based systems. Specific advantages and applications of each category are highlighted herein.
Whitman
Douglas F
DF
Luminex Corporation, 12212 Technology Blvd., Austin, TX 78727, USA. dwhitman@luminexcorp.com
Dunbar
Sherry A
SA
D015342:DNA Probes / Q000235:genetics; D008954:Models, Biological; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes