title: Antinociceptive activity of methanolic extract of Epilobium hirsutum.
abstract: Antinociceptive activity of methanolic extract of aerial parts of Epilobium hirsutum (EH) was determined in the Hot plate and writhing tests in mice. Nearly all extracts showed a dose dependent and marked analgesic activity in mice in the thermal and chemical models of analgesia when compared to the control. Methanol extract at dose of 500 mg kg(-1) showed higher activity (97.7% writhing inhibition) than diclofenac 50 mg kg(-1) i.p., (77.8%, p<0.05) and morphine 5 mg kg(-1) i.p. (91.2%, p<0.05). Methanol extract, in all tested doses (200-500 mg kg(-1)) significantly increased the pain threshold in hot plate test (p<0.05). EH extract at 200 mg kg(-1) showed a similar effect to morphine at 5 mg kg(-1). These finding indicate the potential therapeutic use of methanolic extract of aerial parts of EH as a potent antinociceptive agent. LD50 was obtained 1.5+/-0.1 g kg(-1). EH extract did not induce locomotor impairment in mice at any tested doses.
authors.affiliation: Pharmaceutical Sciences Research Center, Sari School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Pourmorad
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Ebrahimzadeh
authors.forename: Mitra
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Siavash
authors.initials: S
authors.lastname: Yasini
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000700:Analgesics / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D004008:Diclofenac / Q000494:pharmacology; D031666:Epilobium / Q000737:chemistry*; D008297:Male; D008519:Medicine, Traditional; D000432:Methanol; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology
chemical_list: D000700:Analgesics; D010936:Plant Extracts; D004008:Diclofenac; D009020:Morphine; D011794:Quercetin; D019342:Acetic Acid; D000432:Methanol