Analytical sensitivity of air samplers based on uniform point-source exposure to airborne porcine reproductive and respiratory syndrome virus and swine influenza virus.
Research and surveillance activities involving airborne pathogens rely on the capture and enumeration of pathogens suspended in aerosols. The objective of this study was to estimate the analytical sensitivity (detection threshold) of each of 4 air samplers for Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus (SIV). In a 5-min sampling period under controlled conditions, the analytical sensitivity of the AGI-30 (Ace Glass, Vineland, New Jersey, USA), AGI-4 (Ace Glass), SKC BioSampler (SKC, Eighty Four, Pennsylvania, USA), and Midwest Micro-Tek sampler (Midwest Micro-Tek, Brookings, South Dakota, USA) was calculated at 1 x 10(1.1), 1 x 10(1.3), 1 X 10(1.1), and 1 x 10(1.2) median tissue culture infectious dose (TCID50) equivalents for PRRSV and 1 x 10(1.4), 1 x 10(1.1), 1 x 10(1.6), and 1 x 10(1.2) TCID50 equivalents for SIV [per 60 L (5-min sampling period)]. Despite marked differences in sampler design, no statistically significant difference in analytical sensitivity was detected between the samplers for collection of artificially produced aerosols containing cell-culture-propagated PRRSV or SIV.
Hermann
Joseph R
JR
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1250, USA.
Zimmerman
Jeffrey J
JJ
D000336:Aerosols / Q000032:analysis / Q000592:standards*; D000391:Air Microbiology; D000818:Animals; D009980:Influenza A virus / Q000302:isolation & purification*; D009976:Orthomyxoviridae Infections / Q000635:transmission / Q000662:veterinary / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000635:transmission / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000635:transmission / Q000821:virology
D000336:Aerosols