Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry.
Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.
Okamoto
Masanori
M
Growth Regulation Research Group, RIKEN Plant Science Center, Tsurumi, Yokohama, Japan.
Hanada
Atsushi
A
Kamiya
Yuji
Y
Yamaguchi
Shinjiro
S
Nambara
Eiji
E
D000040:Abscisic Acid / Q000737:chemistry* / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D005875:Gibberellins / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D010937:Plant Growth Regulators / Q000737:chemistry*; D010944:Plants / Q000378:metabolism
D005875:Gibberellins; D010937:Plant Growth Regulators; D000040:Abscisic Acid