title: Spread of CTX-M-type extended-spectrum beta-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital.
abstract: This study was to determine the prevalence and characteristics of CTX-M-type extended-spectrum beta-lactamases (ESBLs) in nonduplicate Escherichia coli (n=760) and Klebsiella pneumoniae (n=379) bloodstream isolates collected during January 2005 to October 2007 at a university hospital (2000 beds) in Seoul, Korea. Antimicrobial susceptibilities were determined by disk diffusion and agar dilution methods. The double-disk synergy test detected ESBLs in 8.7% (66/760) of E. coli and 11.3% (43/379) of K. pneumoniae isolates. Polymerase chain reaction detected bla(CTX-M) in 60/66 (90.9%) E. coli and 9/43 (20.9%) K. pneumoniae isolates with the ESBL phenotype. CTX-M-14 was the most common type of CTX-M ESBLs in both E. coli (n=32) and K. pneumoniae (n=6). CTX-M-15 was the 2nd most common type of CTX-M ESBLs in E. coli (n=22), but it was not detected in K. pneumoniae. In addition, CTX-M-24 (n=2), CTX-M-65 (n=2), CTX-M-27 (n=1), and CTX-M-32 (n=1) were detected for the 1st time in Korea.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seodaemun-Gu, Seoul 120-752, South Korea.
authors.forename: Sang-Guk
authors.initials: SG
authors.lastname: Lee
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Hyukmin
authors.initials: H
authors.lastname: Lee
authors.forename: Chang Ki
authors.initials: CK
authors.lastname: Kim
authors.forename: Yangsoon
authors.initials: Y
authors.lastname: Lee
authors.forename: Eunmi
authors.initials: E
authors.lastname: Koh
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
mesh_terms: D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D006761:Hospitals; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D007723:Korea; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D015995:Prevalence; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D001618:beta-Lactamases