Chemical approach toward efficient DNA methylation analysis.
The development of a reaction for the detection of the presence/absence of one methyl group in a very long DNA strand is a chemically and biologically challenging research subject. Several newly designed chemical assays for the typing of DNA methylation are reported and discussed in this paper. A new concept of sequence-specific short-term methylation analysis, supported by a chemical basis, is the starting point for a novel methylation-typing assay, which will supersede conventional methods.
Okamoto
Akimitsu
A
Advanced Science Institute, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, 351-0198, Japan. aki-okamoto@riken.jp
D002621:Chemistry / Q000379:methods*; D002874:Chromosome Mapping; D018899:CpG Islands; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D005260:Female; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D005838:Genotype; D006801:Humans; D008297:Male; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry
D017319:Photosensitizing Agents; D003596:Cytosine; D004247:DNA; D010100:Oxygen