title: Noncovalent immobilization of Pectinesterase (Prunus armeniaca L.) onto bentonite.
abstract: In this work, pectinesterase isolated from Malatya apricot was immobilized onto acid-treated bentonite surface by simple adsorption at pH 9.0. The properties of free and immobilized enzyme were defined. The effect of various factors such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. Optimum pH and temperature were determined to be 9.0 and 50 degrees C, respectively. Kinetic parameters of the immobilized enzyme (Km and Vmax values) were also determined as 0.51 mM of the Km and 14.6 micromol min(-1) mg(-1) of the Vmax. No drastic change was observed in the Km value after immobilization. The Vmax value of immobilized enzyme was 8.4-fold bigger than those of free enzyme. Thermal and storage stability experiments were carried out. The patterns of heat stability indicated that the immobilization process tends to stabilize the enzyme. The properties of the immobilized enzyme were compared to those of the free enzyme.
authors.affiliation: Yildiz Technical University, Faculty of Science and Arts, Department of Chemistry, Esenler, Istanbul, Turkey. karakus@yildiz.edu.tr
authors.forename: Emine
authors.initials: E
authors.lastname: Karakuş
authors.forename: Aynur
authors.initials: A
authors.lastname: Ozler
authors.forename: Sule
authors.initials: S
authors.lastname: Pekyardimci
mesh_terms: D000327:Adsorption; D001546:Bentonite; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D027861:Prunus; D013499:Surface Properties; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D001546:Bentonite; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase