title: Anti-allergic effects of Teucrium japonicum on mast cell-mediated allergy model.
abstract: The mast cell-mediated immediate-type allergic reaction is involved in many allergic diseases such as asthma, allergic rhinitis, and sinusitis. Stimulation of mast cells starts the process of degranulation resulting in release of mediators such as histamine and an array of inflammatory cytokines. In this report, we investigated the effect of aqueous extract of Teucrium japonicum Houttuyn (Labiatae) (AXTJ) on the mast cell-mediated allergy model and studied its possible mechanisms of action. AXTJ inhibited compound 48/80-induced systemic reactions and serum histamine release in mice. AXTJ decreased immunoglobulin E-mediated passive cutaneous anaphylaxis reaction. AXTJ reduced histamine release and intracellular calcium from rat peritoneal mast cells activated by compound 48/80. In addition, AXTJ attenuated activation of nuclear factor (NF)-kappaB, and downstream tumor necrosis factor (TNF)-alpha expression in phorbol 12-myristate 13-acetate and calcium ionophore A23187-stimulated human mast cells. Our findings provide evidence that AXTJ inhibits mast cell-derived allergic reactions and involvement of intracellular calcium, TNF-alpha, and NF-kappaB in these effects.
authors.affiliation: IHBR, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea. shkim72@knu.ac.kr
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Seung-Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Sin-Myoung
authors.initials: SM
authors.lastname: Kang
authors.forename: Hoon
authors.initials: H
authors.lastname: Jeon
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Lim
authors.forename: Taeg Kyu
authors.initials: TK
authors.lastname: Kwon
authors.forename: Won-Hwan
authors.initials: WH
authors.lastname: Park
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D006633:Histamine Antagonists; D006636:Histamine Release / Q000187:drug effects*; D006967:Hypersensitivity / Q000188:drug therapy; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D031367:Teucrium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: D018926:Anti-Allergic Agents; D006633:Histamine Antagonists; D007476:Ionophores; D016328:NF-kappa B; D010703:Phorbol Esters; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C040618:teucrium extract; C008874:phorbol-12,13-diacetate; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium