title: Interaction of the human somatostatin receptor 3 with the multiple PDZ domain protein MUPP1 enables somatostatin to control permeability of epithelial tight junctions.
abstract: The presence of heterotrimeric G-proteins at epithelial tight junctions suggests that these cellular junctions are regulated by so far unknown G-protein coupled receptors. We identify here an interaction between the human somatostatin receptor 3 (hSSTR3) and the multiple PDZ protein MUPP1. MUPP1 is a tight junction scaffold protein in epithelial cells, and as a result of the interaction with MUPP1 the hSSTR3 is targeted to tight junctions. Interaction with MUPP1 enables the receptor to regulate transepithelial permeability in a pertussis toxin sensitive manner, suggesting that hSSTR3 can activate G-proteins locally at tight junctions.
authors.affiliation: Institut für Zellbiochemie und klinische Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Chong Wee
authors.initials: CW
authors.lastname: Liew
authors.forename: Matthias
authors.initials: M
authors.lastname: Vockel
authors.forename: Günter
authors.initials: G
authors.lastname: Glassmeier
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Brandner
authors.forename: Gregorio J
authors.initials: GJ
authors.lastname: Fernandez-Ballester
authors.forename: Jürgen R
authors.initials: JR
authors.lastname: Schwarz
authors.forename: Stefan
authors.initials: S
authors.lastname: Schulz
authors.forename: Friedrich
authors.initials: F
authors.lastname: Buck
authors.forename: Luis
authors.initials: L
authors.lastname: Serrano
authors.forename: Dietmar
authors.initials: D
authors.lastname: Richter
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Kreienkamp
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins; D054731:PDZ Domains; D010539:Permeability / Q000187:drug effects; D037342:Pertussis Toxin / Q000494:pharmacology; D017481:Receptors, Somatostatin / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; C111442:MPDZ protein, human; D008565:Membrane Proteins; D017481:Receptors, Somatostatin; C103631:somatostatin receptor 3; D013004:Somatostatin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins