title: Enhanced secretion of biologically active murine interleukin-12 by Lactococcus lactis.
abstract: The novel signal peptide SLPmod was used for the secretion of murine interleukin-12 (mIL-12) by Lactococcus lactis. A >4-fold increase in secretion was observed when SLPmod was used instead of the Usp45-derived secretion signal. Oral delivery of this cytokine using the autoinducible host L. lactis FI5876 utilizing SLPmod resulted in a significant increase in mIL-12 plasma levels in mice.
authors.affiliation: BBSRC Institute of Food Research, Colney, Norwich, United Kingdom. antonio.fernandezdumont@efsa.europa.eu
authors.forename: Antonio
authors.initials: A
authors.lastname: Fernandez
authors.forename: Nikki
authors.initials: N
authors.lastname: Horn
authors.forename: Udo
authors.initials: U
authors.lastname: Wegmann
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicoletti
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gasson
authors.forename: Arjan
authors.initials: A
authors.lastname: Narbad
mesh_terms: D000818:Animals; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010949:Plasma / Q000737:chemistry; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D018664:Interleukin-12