[Application of orthogonal design in tissue culture of Dioscorea opposite].
OBJECTIVE
To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose.


METHODS
stems were selected as explants. The effects of three kinds of factors were studied by orthogonal design method including sucrose, 6-BA, NAA on the buds and microtuber induction. The data were analyzed with range analysis and vadance analysis. RESULT AND CONDUSION: The optimal media to induce many buds from stems were MS + 6-BA 1 mg x L(-1) + NAA 1 mg x L(-1) + sucrose 3%, the effect of the three factors was in sequence of sucrose >6-BA > NAA. The optimal media to induce microtuber from stems were MS+6-BA 1.5 mg x V1 +NAA 1.5 mg x L(-1) + sucrose 5%, the effect of the three factors was in sequence of sucrose >6-BA > NAA.
Xue
Jian-ping
JP
Department of Biology, Huaibei Coal Industry Teachers' College, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China. xuejp2000@yahoo.com.cn
Zhao
Xiao-quan
XQ
Zhang
Ai-min
AM
Huang
Yue-qin
YQ
D003470:Culture Media; D027742:Dioscorea / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators; D013395:Sucrose