Tissue array for Tp53, C-myc, CCND1 gene over-expression in different tumors.
OBJECTIVE
To rapidly detect molecular alterations in different malignancies and investigate the possible role of Tp53, C-myc, and CCND1 genes in development of tumors in human organs and their adjacent normal tissues, as well as the possible relation between well- and poorly-differentiated tumors.


METHODS
A tissue array consisting of seven different tumors was generated. The tissue array included 120 points of esophagus, 120 points of stomach, 80 points of rectum, 60 points of thyroid gland, 100 points of mammary gland, 80 points of liver, and 80 points of colon. Expressions of Tp53, C-myc, and CCND1 were determined by RNA in situ hybridization. 3' terminal digoxin-labeled anti-sense single stranded oligonucleotide and locked nucleic acid modifying probe were used.


RESULTS
The expression level of Tp53 gene was higher in six different carcinoma tissue samples than in paracancerous tissue samples with the exception in colon carcinoma tissue samples (P < 0.05). The expression level of CCND1 gene was significantly different in different carcinoma tissue samples with the exception in esophagus and colon carcinoma tissue samples. The expression level of C-myc gene was different in esophagus carcinoma tissue samples (chi2 = 18.495, P = 0.000), stomach carcinoma tissue samples (chi2 = 23.750, P = 0.000), and thyroid gland tissue samples (chi2 = 10.999, P = 0.004). The intensity of signals was also different in different carcinoma tissue samples and paracancerous tissue samples.


CONCLUSIONS
Over-expression of the Tp53, CCND1, and C-myc genes appears to play a role in development of human cancer by regulating the expression of mRNA. Tp53, CCND1 and C-myc genes are significantly correlated with the development of different carcinomas.
Liu
Guo-Yan
GY
Department of General Surgery, Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361004, Fujian Province, China.
Luo
Qi
Q
Xiong
Bin
B
Pan
Chao
C
Yin
Ping
P
Liao
Hong-Feng
HF
Zhuang
Wei-Chun
WC
Gao
Hong-Zhi
HZ
D001943:Breast Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism; D002681:China; D003110:Colonic Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012004:Rectal Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism; D046888:Tissue Array Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
C530104:CCND1 protein, human; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1