[Correlation between expression of metastasis-associated gene 1 and matrix metalloproteinase 9 and invasion and metastasis of breast cancer].
OBJECTIVE
To investigate the expression of metastasis-associated gene 1 (MTA1) and matrix metalloproteinase 9 (MMP-9) gene in breast carcinoma and the relationship between the expression of these genes and the invasion of breast cancer.


METHODS
Fluorescence quantitative PCR technique was used to detect the mRNA expression of MTA1 and MMP-9 gene among 56 human breast cancer samples.


RESULTS
The mRNA expression rate of MTA1 was higher in 83.9% (47/56) of the primary breast cancer tissues compared with the matched normal breast tissues. The mRNA expression of MMP-9 was higher in 85.7% (48/56) of the primary breast cancer tissues compared to the matched normal breast tissues. The over-expression of MTA1 and that of the MMP-9 gene were significantly related with the degree of differentiation, clinical stage, and lymph node metastasis of breast cancer (all P < 0.05). The positive ratio of MTA1 gene is tightly associated with that of MMP-9 gene (P < 0.05).


CONCLUSIONS
The united detection of MTA1 and MMP-9 gene expression predicts the invasion and metastasis of breast cancer and supplies evidence for clinical therapy and judgment of prognosis. MTA1 and MMP-9 will become new targets for gene therapy.
Li
Jing-ruo
JR
Department of Breast Disease Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Li
Meng-quan
MQ
Bao
Jun-tao
JT
Li
Jian-zhang
JZ
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000235:genetics*; D006801:Humans; D008207:Lymphatic Metastasis; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators
C089320:MTA1 protein, human; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators; D020780:Matrix Metalloproteinase 9; D006655:Histone Deacetylases