Highly sensitive method for determination of N-nitrosamines using high-performance liquid chromatography with online UV irradiation and luminol chemiluminescence detection.
A new method for the measurement of N-nitrosamines in part-per-trillion concentrations from water samples without preconcentration steps has been developed. This method is based on online UV irradiation after high-performance liquid chromatographic separation and subsequent luminol chemiluminescence detection without addition of an oxidant. It was confirmed that N-nitrosamines in basic aqueous solution were transformed to peroxynitrite by UV irradiation. The detection limits for this method were 1.5 ng/L, 2.9 ng/L, 3.0 ng/L, and 2.7 ng/L for N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine, respectively, at a signal-to-noise ratio of 3. The calibration graphs were linear in the range of 5-1000 ng/L for these N-nitrosamines. This method was used for the determination of N-nitrosamines in tap water, river water, and industrial plant effluent samples. The recoveries of N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine present in tap water sample at a concentration of 10 ng/L (mean+/-standard deviation, n=4) were (94.8+/-2.7)%, (102.0+/-6.9)%, (99.3+/-3.9)%, and (102.8+/-2.5)%, respectively. These results indicate that our proposed method can be applied satisfactorily to the determination of N-nitrosamines in water samples.
Kodamatani
Hitoshi
H
Faculty of Science, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, Japan. kodama@sci.kagoshima-u.ac.jp
Yamazaki
Shigeo
S
Saito
Keiitsu
K
Amponsaa-Karikari
Abena
A
Kishikawa
Naoya
N
Kuroda
Naotaka
N
Tomiyasu
Takashi
T
Komatsu
Yu
Y
D002851:Chromatography, High Pressure Liquid; D004128:Dimethylnitrosamine; D008163:Luminescent Measurements; D008165:Luminol / Q000032:analysis* / Q000737:chemistry; D009242:N-Nitrosopyrrolidine; D009602:Nitrosamines / Q000032:analysis*; D030421:Peroxynitrous Acid / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000032:analysis*
D009602:Nitrosamines; D014874:Water Pollutants, Chemical; D030421:Peroxynitrous Acid; C002741:N-nitrosomorpholine; D008165:Luminol; D004128:Dimethylnitrosamine; D009242:N-Nitrosopyrrolidine