Determination of ammonium using a microplate-based fluorometric technique.
The determination of ammonium (NH(4)(+)) in concentrations ranging from nanomolar to micromolar in fresh and brackish waters often loaded in high suspended particulate matter and dissolved organic acids is presented. The newly described microplate-based fluorometric technique is allowing quick automated readings of different groups of samples with different background fluorescence and matrix effects. The lowest detectable concentration was estimated to 5nM using the average detected blank +/-3S.D. and the practical detection limit (LOD) determined with successive calibration curves was 50nM with an excellent repeatability. High loading of suspended particular matter, coloured organic acids, and salinity changes were not interfering with the accurate determination of ammonium. To illustrate its robustness and efficiency, this technique has been applied to water samples taken from rivers, saltmarshes and estuaries, spanning a large range of ammonium levels and chemical properties. Measurements of ammonium on reddish turbid waters sampled in south shore of St. Lawrence Estuary showed ammonium concentrations between 0.05+/-0.01 and 3.89+/-0.03muM, indicating a significant source of ammonium from terrestrial and saltmarsh ecosystems.
Poulin
Patrick
P
Institut des Sciences de la Mer de Rimouski (ISMER), Université du Québec à Rimouski, 310 allée des Ursulines, Rimouski, Que., Canada G5L 3A1.
Pelletier
Emilien
E