Development of a gel permeation chromatographic assay to achieve mass balance in cellulose acetate phthalate stability studies.
Cellulose acetate phthalate (CAP, cellulose acetate 1,2-benzenedicarboxylate) is a common polymeric oral tablet coating. CAP is also a vaginal microbicide candidate that potently inhibits HIV-1 proliferation. This paper describes the development of a precise, stability-indicating gel permeation chromatography (GPC) assay for CAP. During accelerated stability studies monitored by separate reversed-phase high performance liquid chromatography (RP-HPLC) and GPC analyses, an apparent loss of mass balance was observed. This deficit was corrected by recalculating the response factor (RF) for each degraded sample, proportional to the fraction of phthalate remaining bound to the polymeric CAP. The correction factor enabled CAP and the degradation product phthalic acid (PA) to be quantitated by a single GPC analysis. The chromatographic approach taken here could potentially apply to any polymer containing degradable chromophores.
Mayhew
James W
JW
Dow Pharmaceutical Sciences, Inc., 1330 Redwood Way, Petaluma, CA 94954, USA.
Gideon
Lulu T
LT
Ericksen
Bryan
B
Hlavaty
John J
JJ
Yeh
Simon M
SM
Chavdarian
Charles G
CG
Strick
Nathan
N
Neurath
A Robert
AR
D019380:Anti-HIV Agents / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D002850:Chromatography, Gel / Q000191:economics / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005260:Female; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis; D015394:Molecular Structure; D015203:Reproducibility of Results; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D019380:Anti-HIV Agents; D012996:Solutions; D012997:Solvents; D002482:Cellulose; C011708:cellulose acetate phthalate