title: A stable prefusion intermediate of the alphavirus fusion protein reveals critical features of class II membrane fusion.
abstract: Alphaviruses infect cells via a low-pH-triggered membrane fusion reaction mediated by the class II virus fusion protein E1, an elongated molecule with three extramembrane domains (DI-III). E1 drives fusion by inserting its fusion peptide loop into the target membrane and refolding to a hairpin-like trimer in which DIII moves toward the target membrane and packs against the central trimer. Three-dimensional structures provide static pictures of prefusion and postfusion E1 but do not explain this transition. Using truncated forms of E1, we reconstituted a low-pH-dependent intermediate composed of trimers of DI/II. Unexpectedly, DI/II trimers were stable in the absence of DIII. Once formed at a low pH, DI/II trimers efficiently and specifically bound recombinant DIII through a pH-independent reaction. Even in the absence of DIII, DI/II trimers interacted to form hexagonal lattices and to cause membrane deformation and tubulation. These studies identify a prefusion intermediate in class II membrane fusion.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Sánchez-San Martín
authors.forename: Hernando
authors.initials: H
authors.lastname: Sosa
authors.forename: Margaret
authors.initials: M
authors.lastname: Kielian
mesh_terms: D000524:Alphavirus / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D011485:Protein Binding; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D053586:Virus Internalization
chemical_list: D046911:Macromolecular Substances; D014760:Viral Fusion Proteins