[Expression feature and bioinformatic analysis of molar root patterning gene 1].
OBJECTIVE
To examine the expression of molar root patterning gene 1 (Mrp1) and predict the Mrp1 structure by bioinformatics analysis.


METHODS
A pair of Mrp1-specific PCR primers were designed, and RT-PCR method was used to study the mRNA's expression pattern in rat molar root and other organs. Gene positioning and other protein sequence prediction were carried out by chromosome analysis and other bioinformatics analysis.


RESULTS
Mrp1 was expressed not only in the molar but also in the developing pancreas, liver, lung and kidney tissues. Mrp1 was located in the 18q12.3 chromosome of the rats and the Mrp1 amino acids sequence had about 37% homology with a known protein Uroplakin IIIb (p35) which was an urothelial differentiation membrane molecular marker. A trans-membrane structure, 5 PKC phosphorylation sites and 4 CKII phosphorylation sites in Mrp1 were found.


CONCLUSIONS
Mrp1 has a broad expression in different developing organs, and it may have a important function in the rat tooth root development.
Xing
Xiang-hui
XH
Department of Oral Histopathology, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wang
Xiao-jing
XJ
Yu
Shi-bin
SB
Xuan
Kun
K
Jin
Yan
Y
Wen
Ling-ying
LY
D000818:Animals; D019295:Computational Biology; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D008963:Molar / Q000254:growth & development*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014092:Tooth Root
D021382:Protein Sorting Signals; D011506:Proteins