title: [Effect of transfection of LKB1 on biological behavior of human lung adenocarcinoma cells].
abstract: OBJECTIVE
  To determine the effects of exogenous LKB1 gene on biological behavior of lung carcinoma cells.
  
  
  METHODS
  Nest PCR was used to clone LKB1 gene pcDNA-LKB1 and pcDNA3.1 were introduced into A549 cell line by lipofectin transfection, and the A549 cells stably expressing LKB1 gene were established by G418 selection. The expression of LKB1 and STAT3 protein was detected by Western blot. Cell proliferation was observed by growth curve and clone formation. Cell cycle and apoptosis were assayed by flow cytometry(FCM).
  
  
  RESULTS
  A549 cells stably expressing LKB1 protein were established. Compared with the vector transfected cells, the positive clone cells grew more slowly. FCM showed that more positive clone cells went into phase G0/G1 and fewer cells went into phase S. Moreover, there was significant difference on apoptosis between the two group cells.
  
  
  CONCLUSIONS
  LKB1 protein might inhibit malignant biological behavior of A549 cells partly by down-regulating the tyrosine-phosphorylated level of STAT3 protein.
authors.affiliation: Department of Oncology, Medical College, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liang
authors.forename: Ke-Jun
authors.initials: KJ
authors.lastname: Nan
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Yao
authors.forename: Tao
authors.initials: T
authors.lastname: Tian
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Wang
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases