title: Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2.
abstract: Both Mycobacterium tuberculosis and Mycobacterium bovis can produce tuberculosis in humans. Mycobacterium tuberculosis has low nitrate reductase activity during aerobic growth (AG), but shows strong hypoxic induction. Virulent M. bovis has weak activity during AG with no hypoxic induction. Bacille Calmette-Guerin (BCG) lacks activity in both stages. Transcription of narG of the nitrate reductase enzyme operon was higher in M. tuberculosis than in M. bovis or BCG. Transcription of narK2 encoding the nitrate transporter was induced by hypoxia in M. tuberculosis but not M. bovis or BCG. Insertion of the M. tuberculosis narGHJI operon into M. bovis resulted in increased activity only during AG. Regulation of both the nitrate reductase enzyme and transporter are regulated differently in the two species.
authors.affiliation: Tuberculosis Research Laboratory, Department of Veterans Affairs Medical Center, Long Beach, CA 90822, USA. chuck@sohaskey.com
authors.forename: Charles D
authors.initials: CD
authors.lastname: Sohaskey
authors.forename: Lucia
authors.initials: L
authors.lastname: Modesti
mesh_terms: D000332:Aerobiosis; D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009163:Mycobacterium bovis / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism*; D000091285:Nitrate Transporters; D009566:Nitrates / Q000378:metabolism*; D009876:Operon; D010084:Oxidation-Reduction; D014376:Tuberculosis / Q000382:microbiology; D014774:Virulence
chemical_list: D027321:Anion Transport Proteins; D001426:Bacterial Proteins; D000091285:Nitrate Transporters; D009566:Nitrates; D050901:Nitrate Reductase