Plasma from preeclamptic women stimulates transendothelial migration of neutrophils.
Neutrophils infiltrate systemic vasculature of women with preeclampsia, so we tested the hypothesis that factors in plasma of preeclamptic women activate endothelial cells to produce IL-8 resulting in transendothelial migration of neutrophils. Neutrophil migration was studied using the Transwell system. An endothelial cell line was grown to confluence on the inserts and treated with 10% plasma from normal nonpregnant (NNP), normal pregnant (NP) and preeclamptic (PE) women or with an oxidizing solution containing linoleic acid (OxLA). Compared to medium control, NNP plasma or NP plasma, PE plasma significantly stimulated IL-8 and neutrophil migration which was inhibited by vitamins E and C or IL-8 neutralizing antibody. Compared to medium control or LA, OxLA stimulated IL-8 and neutrophil migration which was inhibited by vitamins E and C or IL-8 antibody.


CONCLUSIONS
Factors present in plasma of preeclamptic women stimulate transendothelial migration of neutrophils which is due to induction of oxidative stress and production of IL-8.
Walsh
Scott W
SW
Department of Obstetrics and Gynecology, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298, USA. swwalsh@vcu.edu
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002460:Cell Line; D054441:Cell Migration Assays, Leukocyte; D002465:Cell Movement / Q000187:drug effects; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; C508606:CXCL8 protein, human; D016209:Interleukin-8; D014810:Vitamin E; D019787:Linoleic Acid; D001205:Ascorbic Acid