Mycetocola reblochoni sp. nov., isolated from the surface microbial flora of Reblochon cheese.
Four Gram-positive, aerobic, non-sporulating, rod-shaped bacteria isolated from the surface microflora of Reblochon cheese at the late stage of ripening had chemotaxonomic properties characteristic of members of the family Microbacteriaceae. The isolates had virtually identical SDS-PAGE whole-organism protein patterns, shared many chemical and phenotypic characteristics and formed an independent branch in the Microbacteriaceae 16S rRNA gene tree that was most closely related to the type strains of Mycetocola species. The new isolates had chemotaxonomic properties consistent with their classification in the genus Mycetocola but were readily distinguished from recognized members of this taxon based on DNA-DNA relatedness, whole-organism protein and phenotypic data. The combined genotypic and phenotypic data indicate that the isolates should be classified in the genus Mycetocola as members of a novel species, for which the name Mycetocola reblochoni sp. nov. is proposed. The type strain is LMG 22367(T) (=R-20377(T) =BRB-1L41(T) =DSM 18580(T)).
Bora
Nagamani
N
School of Biology, Ridley Building, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
Vancanneyt
Marc
M
Gelsomino
Roberto
R
Snauwaert
Cindy
C
Swings
Jean
J
Jones
Amanda L
AL
Ward
Alan C
AC
Chamba
Jean-Francois
JF
Kroppenstedt
Reiner M
RM
Schumann
Peter
P
Goodfellow
Michael
M
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D002611:Cheese / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D024482:Vitamin K 2 / Q000032:analysis
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2