title: Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast.
abstract: We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological aging as compared to WT. Expression of genes encoding subunits of mitochondrial electron transport chain and ATP synthase is significantly downregulated in HsTnII, and as a consequence, HsTnII is not able to respire ethanol. All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis.
authors.affiliation: Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium.
authors.forename: An M
authors.initials: AM
authors.lastname: Aerts
authors.forename: Piotr
authors.initials: P
authors.lastname: Zabrocki
authors.forename: Gilmer
authors.initials: G
authors.lastname: Govaert
authors.forename: Janick
authors.initials: J
authors.lastname: Mathys
authors.forename: Didac
authors.initials: D
authors.lastname: Carmona-Gutierrez
authors.forename: Frank
authors.initials: F
authors.lastname: Madeo
authors.forename: Joris
authors.initials: J
authors.lastname: Winderickx
authors.forename: Bruno P A
authors.initials: BP
authors.lastname: Cammue
authors.forename: Karin
authors.initials: K
authors.lastname: Thevissen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D017346:Protein Serine-Threonine Kinases; C530964:SCH9 protein, S cerevisiae