Circular dichroism studies of type III collagen mimetic peptides with anti- or pro-aggregant activities on human platelets.
We report the synthesis of collagen related peptides containing the peptide sequence Lys-Hyp-Gly-Glu-Hyp-Gly-Pro-Lys. The anti-thrombotic activity effects of different glycine mutations in this sequence were studied in regard with their different adopted conformations. The biological results could be correlated to the glycine propensity to adopt a more stable polyproline II helix conformation. The incorporation of these sequences in "collagen-like" alpha-triple-helix peptides shows a pro-thrombotic activity compared to a scrambled negative control peptide which possesses no significant activity.
Pêcher
Julien
J
Laboratoire des Glucides, UMR-CNRS 6219, Faculté de Pharmacie, Université de Picardie Jules Verne, 1 rue des louvels, F-80037 Amiens, France.
Pires
Viviane
V
Djaafri
Ibtissem
I
Da Nascimento
Sophie
S
Fauvel-Lafève
Françoise
F
Legrand
Chantal
C
Sonnet
Pascal
P
D001792:Blood Platelets / Q000187:drug effects; D002942:Circular Dichroism / Q000379:methods; D024061:Collagen Type III / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D006801:Humans; D018716:Molecular Mimicry; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D013997:Time Factors
D024061:Collagen Type III; D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors