[Study of inhibition effect of zinc-, iron- and manganese-saturated bovine lactoferrin on hepatitis B virus DNA in vitro].
OBJECTIVE
To probe inhibition effect of zinc-saturated (Zn2+ -BLF), iron-saturated (Fe2+ -BLF) and manganese-saturated (Mn2+ -BLF) bovine lactoferrin on hepatitis B virus DNA (HBV-DNA) in vitro.


METHODS
Nature HBV was used to infect HepG2 cell, HBV-DNA was measured by fluorescence polymerase chain reaction to detect the effect of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF, the MTT test was used to examine cytotoxic effect of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF.


RESULTS
The maximum nontoxic dose (TD0) in HepG2 cell of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF were 1.5,3.0 and 1.5 g/L respective. After HepG2 cell were infected with HBV, every Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF test group had effect on inhibiting HBV-DNA to some extent. Compared the inhibition effect of concentration of 1.5 g/ L test group in each term, the inhibition result of Zn2 -BLF was 5.746 +/- 0.114, the inhibition result of Fe2+ -BLF was 6. 446 +/- 0.103, and the inhibition result of Mn2+ -BLF was 5.999 +/- 0.725.


CONCLUSIONS
After HepG2 cell was infected with HBV, HBV-DNA could be inhibited by Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF. Zn2+ -BLF has higher inhibition effect than Mn2+ -BLF, and Fe2+ -BLF has the lowest inhibition effect, but the possible mechanism need to be lucubrated.
Zhou
Haibo
H
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Li
Songtao
S
Huang
Guirong
G
Liu
Ning
N
D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000187:drug effects*; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000494:pharmacology; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D008345:Manganese / Q000737:chemistry / Q000494:pharmacology; D008670:Metals / Q000737:chemistry / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
D000998:Antiviral Agents; D004279:DNA, Viral; D008670:Metals; D008345:Manganese; D007501:Iron; D007781:Lactoferrin; D015032:Zinc