Determination of functional gamma-GTase genes and investigation of the biological activity of proteins in Arabidopsis thaliana at different stages of growth.
The results in the present study emphasize that in Arabidopsis thaliana genome, gamma-GTase encoded by a gene family comprised of four genes. Three of these genes are functionally expressed while the fourth is a pseudogene. The three functional genes express different active protein isoforms that appeared to have different functions. Two genes (GGT1 and GGT2) are structurally similar and expresses proteins with the same molecular weight and both target to the same cellular compartment (plasma membrane). However, both demonstrated different tissue localization, physiological activities and different patterns of response to environmental stress. The majority of GGTI encoded by the first gene was localized in the rosette leaves, It is also expressed throughout the whole plant.
Radwan
Safaa
S
Department of Botany, Faculty of Science, Tanta University, 31527 Tanta, Egypt.
Badr
Salwa
S
Badr
Abdelfattah
A
Oliver
David
D
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015139:Blotting, Southern; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D020033:Protein Isoforms / Q000235:genetics; D000637:Transaminases / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D020033:Protein Isoforms; C520630:At1g23310 protein, Arabidopsis; D000637:Transaminases