Spectroscopic studies on the interaction of hypocrellin A and hemoglobin.
The spectrophotometric and spectrofluorimetric studies revealed that hemoglobin (Hb) could interact with hypocrellin A, a photosensitizing drug used in photodynamic therapy. It was found that this kind of interaction can induce the conformational changes in Hb. In addition, based on fluorescence quenching titration and electron paramagnetic resonance spectroscopy results, the binding parameters, thermodynamic parameters are obtained. The quenching mechanism is also proposed.
Zhou
Jiahong
J
Analysis & Testing Center, Jiangsu Engineering Research Center for Bio-medical Function Materials, Nanjing Normal University, 122 Ninghai Road, Nanjing 210097, PR China. zhoujiahong@njnu.edu.cn
Wu
Xiaohong
X
Gu
Xiaotian
X
Zhou
Lin
L
Song
Kaixi
K
Wei
Shaohua
S
Feng
Yuying
Y
Shen
Jian
J
D004578:Electron Spin Resonance Spectroscopy; D006454:Hemoglobins / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D019800:Phenol; D011809:Quinones / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis
D006454:Hemoglobins; D011809:Quinones; D012996:Solutions; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A