title: Growth and osteogenic differentiation of adipose stem cells on PLA/bioactive glass and PLA/beta-TCP scaffolds.
abstract: The aim of this study was to compare the effects of novel three-dimensional composite scaffolds consisting of a bioactive phase (bioactive glass or beta-tricalcium phosphate [beta-TCP] 10 and 20 wt%) incorporated within a polylactic acid (PLA) matrix on viability, distribution, proliferation, and osteogenic differentiation of human adipose stem cells (ASCs). The viability and distribution of ASCs on the bioactive composite scaffolds was evaluated using Live/Dead fluorescence staining, environmental scanning electron microscopy, and scanning electron microscopy. There were no differences between the two concentrations of bioactive glass and beta-TCP in PLA scaffolds on proliferation and osteogenic differentiation of ASCs. After 2 weeks of culture, DNA content and alkaline phosphatase (ALP) activity of ASCs cultured on PLA/beta-TCP composite scaffolds were higher relative to other scaffold types. Interestingly, the cell number was significantly lower, but the relative ALP/DNA ratio of ASCs was significantly higher in PLA/bioactive glass scaffolds than in other three scaffold types. These results indicate that the PLA/beta-TCP composite scaffolds significantly enhance ASC proliferation and total ALP activity compared to other scaffold types. This supports the potential future use of PLA/beta-TCP composites as effective scaffolds for tissue engineering and as bone replacement materials.
authors.affiliation: Regea Institute for Regenerative Medicine, University of Tampere, Tampere, Finland. suvi.haimi@regea.fi
authors.forename: Suvi
authors.initials: S
authors.lastname: Haimi
authors.forename: Niina
authors.initials: N
authors.lastname: Suuriniemi
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Haaparanta
authors.forename: Ville
authors.initials: V
authors.lastname: Ellä
authors.forename: Bettina
authors.initials: B
authors.lastname: Lindroos
authors.forename: Heini
authors.initials: H
authors.lastname: Huhtala
authors.forename: Sari
authors.initials: S
authors.lastname: Räty
authors.forename: Hannu
authors.initials: H
authors.lastname: Kuokkanen
authors.forename: George K
authors.initials: GK
authors.lastname: Sándor
authors.forename: Minna
authors.initials: M
authors.lastname: Kellomäki
authors.forename: Susanna
authors.initials: S
authors.lastname: Miettinen
authors.forename: Riitta
authors.initials: R
authors.lastname: Suuronen
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D005898:Glass / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D054457:Tissue Scaffolds
chemical_list: D000954:Antigens, Surface; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C485817:beta-tricalcium phosphate; D019344:Lactic Acid; C033616:poly(lactide); D004247:DNA; D000469:Alkaline Phosphatase