Precise and accurate quantitative (13)C NMR with reduced experimental time.
In order to detect small variations in (13)C isotopomers concentrations, high sensitivity, accuracy and precision have to be achieved. To assess such criteria, when using (13)C NMR, (13)C bi-labelled ethanol has been proposed as a molecular probe. Advantage has been taken of the pre-established structural relationship between the peak areas of the (13)C NMR spectrum of this molecule, i.e. the ratio of signal areas is set to a fixed value. It is shown that the quality performance, required by quantitative (13)C NMR spectroscopy, is not affected by a large reduction of the repetition delay using relaxation reagents.
Caytan
Elsa
E
Nantes University, LAIEM-UMR-CNRS 6006, Faculty of Science and Technology, 2 rue de la Houssinière, BP 92208, F-44322 Nantes Cedex 3, France.
Remaud
Gérald S
GS
Tenailleau
Eve
E
Akoka
Serge
S