title: Determination of phenolic acids using Trametes versicolor laccase.
abstract: Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1-1.0muM caffeic acid, 0.05-0.2muM ferulic acid, 2.0-14.0muM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-30.0muM caffeic acid, 2.0-10.0muM ferulic acid, 4.0-30.0muM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova-Izmir, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Odaci
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.forename: Nurdan
authors.initials: N
authors.lastname: Pazarlioglu
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Montereali
authors.forename: Walter
authors.initials: W
authors.lastname: Vastarella
authors.forename: Roberto
authors.initials: R
authors.lastname: Pilloton
authors.forename: Azmi
authors.initials: A
authors.lastname: Telefoncu