Neutral polar methacrylate-based monoliths for normal phase nano-LC and CEC of polar species including N-glycans.
Neutral diol methacrylate-based monoliths were developed for normal phase chromatography (NPC) and NP-CEC of polar compounds including N-glycans. Four different diol methacrylate-based monoliths were synthesized via the copolymerization of a functional monomer using either glyceryl monomethacrylate (GMM) or glycidyl methacrylate (GMA) and a crosslinker either ethylene dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM). While the GMM-based monoliths yield in one reaction step polar diol methacrylate monoliths that are ready for use in NPC or NP-CEC, the GMA-based monoliths required a postmodification with hot sulfuric acid to convert the epoxy functions into diols before use in NPC or NP-CEC. All the four monoliths are neutral and void of fixed charges on their surfaces but yet exhibited relatively strong EOF in NP-CEC. The EOF is attributed to the adsorption of ions from the mobile phase thus forming the electric double layer necessary for producing a bulk mobile phase flow. Under the same in situ copolymerization conditions of GMM or GMA with either EDMA or TRIM, the GMM-EDMA monolith was the best choice in terms of retention, separation efficiency, EOF velocity in CEC and linear flow velocity in Nano-LC.
Zhong
Hengwen
H
Department of Chemistry, Oklahoma State University, Stillwater, OK 74078-3071, USA.
El Rassi
Ziad
Z
D000327:Adsorption; D053801:Capillary Electrochromatography; D002851:Chromatography, High Pressure Liquid; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D036103:Nanotechnology; D011134:Polysaccharides / Q000737:chemistry*; D013499:Surface Properties
D008689:Methacrylates; D011134:Polysaccharides