title: Ultrasonic intensity microscopy for imaging of living cells.
abstract: Ultrasound intensity microscopy was developed for in vivo imaging. This paper describes the preliminary results obtained using 300 MHz ultrasound intensity microscopy for in vitro characterization of cell cultures. The novelty of the approach lies in the fact that it allows remote, non-contact and disturbance-free imaging of cultured synovial cells and the changes in the cells' properties due to external stimulants such as transforming growth factor beta-1 (TGF-beta1). The intensity imaging method has potential for extracting mechanical cell properties and monitoring the effects of drugs. Ultrasound propagates through a thin specimen such as cultured cells and is reflected at the interface between the specimen and substrate. A two-dimensional distribution of the ultrasonic intensity, which is closely related to the mechanical properties, is visualized to analyze cell organs, such as the nucleus at the central part and the cytoskeleton at the peripheral zone. After stimulation with TGF-beta1, the ultrasonic intensity at the actin zone was significantly increased compared with the control.
authors.affiliation: Department of Orthopaedic Surgery, Tohoku University School of Medicine, Aobaku, Sendai 980-8574, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Saijo
authors.forename: Akira
authors.initials: A
authors.lastname: Ando
authors.forename: Eiichi
authors.initials: E
authors.lastname: Chimoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Suda
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Onoda
authors.forename: Eiji
authors.initials: E
authors.lastname: Itoi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D008297:Male; D033401:Microscopy, Acoustic / Q000295:instrumentation*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013583:Synovial Membrane / Q000166:cytology* / Q000000981:diagnostic imaging*