Bacillus pallidus sp. nov., isolated from forest soil.
A Gram-positive bacterium, designated strain CW 7(T), was isolated from forest soil in Anhui Province, south-east China. Cells were strictly aerobic, motile with peritrichous flagella and rod-shaped. The strain grew optimally at 30-37 degrees C and pH 7.0-8.0. The major fatty acids of strain CW 7(T) were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). The predominant menaquinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The G+C content of the genomic DNA was 42.3 mol%. Phylogenetic analysis indicated that strain CW 7(T) belonged to a monophyletic cluster within the genus Bacillus and showed 16S rRNA gene sequence similarities of less than 96.5 % to recognized species of the genus Bacillus. The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analyses, showed that strain CW 7(T) represents a novel species of the genus Bacillus, for which the name Bacillus pallidus sp. nov. is proposed. The type strain is CW 7(T) (=KCTC 13200(T)=CCTCC AB 207188(T)=LMG 24451(T)).
Zhou
Yu
Y
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
Wei
Wei
W
Che
Qiaolin
Q
Xu
Yuxin
Y
Wang
Xu
X
Huang
Xing
X
Lai
Ren
R
D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity; D014197:Trees / Q000382:microbiology*
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S