Irradiation-induced loss of microglia in the young brain.
Irradiation-induced loss of neural stem and progenitor cells may contribute to cognitive deficits. Furthermore, subsequent inflammation inhibits neural progenitor cell differentiation. Here we have characterized the microglia response after a single dose of 8 Gy to the brains of postnatal day 9 or 21 rats. The number of Iba-1-positive microglia increased 6 h after IR but had decreased 7 days later, below control levels, and this decrease was more pronounced in P9 rats. Active caspase-3 and TUNEL staining revealed irradiation-induced microglia death. This age-dependent IR-induced loss of microglia likely affects both the response to IR and further brain development.
Kalm
Marie
M
Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, University of Gothenburg, Box 432, SE 405 30, Sweden.
Lannering
Birgitta
B
Björk-Eriksson
Thomas
T
Blomgren
Klas
K
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology* / Q000254:growth & development / Q000528:radiation effects; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D005260:Female; D020287:In Situ Nick-End Labeling / Q000379:methods; D007211:Indoles; D008297:Male; D008840:Microfilament Proteins; D017628:Microglia / Q000528:radiation effects*; D011827:Radiation; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism
C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D007211:Indoles; D008840:Microfilament Proteins; D012097:Repressor Proteins; C525546:Tip30 protein, mouse; D025521:Tumor Suppressor Proteins; C007293:DAPI