title: Effects of resorcylidene aminoguanidine (RAG) on selected parameters of isolated rat liver mitochondria.
abstract: In the present investigation, we attempted to study possible mechanisms of the interactions of resorcylidene aminoguanidine (RAG), the agent with a recognized anti-glycation and antioxidative activity, with rat liver mitochondria. We hypothesized that RAG affects organization of the lipid bilayer in mitochondrial membranes and thus impairs transmembrane Ca(2+) redistribution, transmembrane potential, and respiration capacity. Isolated mitochondria were exposed to RAG (50-200 microM) and several parameters of their function monitored employing spectrofluorimetric, cytometric, and respirometric techniques. Mitochondrial membrane potential and membrane fluidity were tracked using the staining with rhodamine 123 (Rh123) and 1,6-diphenyl-1,3,5-hexatriene (DPH), respectively. Mitochondrial respiration and oxidative phosphorylation was monitored with a high-resolution respirometry, and mobilization of Ca(2+) was detected using spectrofluorimetry with Calcium Green 5-N. RAG depolarized and fluidized mitochondrial membrane, as deduced from reduced fluorescence of intramitochondrial Rh123 and decreased DPH fluorescence anisotropy. The slight inhibitory effect of 100-200 microM RAG on mitochondrial respiratory capacity was observed merely when monitored in the presence of ADP. The reduced sensitivity of mitochondria to calcium-induced depolarization was significant only at higher RAG concentrations (100-200 microM). Moreover, RAG induced pronounced conformational changes in two model proteins: bovine serum albumin and cytochrome c. These findings indicate that regardless of its depolarizing and fluidizing properties, RAG does not largely affect the mitochondrial respiration, although it may significantly lower oxidative phosphorylation when used at higher concentrations.
authors.affiliation: Department of General Biophysics, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland. magdalab@biol.uni.lodz.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Labieniec
authors.forename: Tomasz
authors.initials: T
authors.lastname: Przygodzki
authors.forename: Jozef
authors.initials: J
authors.lastname: Cársky
authors.forename: Dominika
authors.initials: D
authors.lastname: Malinska
authors.forename: Jacek
authors.initials: J
authors.lastname: Rysz
authors.forename: Cezary
authors.initials: C
authors.lastname: Watala
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006146:Guanidines / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D006146:Guanidines; D008051:Lipid Bilayers; C115168:resorcylidene aminoguanidine; D012710:Serum Albumin, Bovine; D014364:Tryptophan; D045304:Cytochromes c; D010100:Oxygen; D002118:Calcium