title: Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba.
abstract: Six strains were isolated from root nodules of Vicia faba grown in Nanchang, Yifeng, Taihu, Huaibei, Bengbu and Lujiang, in the middle and lower reaches of the Yangtze River. According to phylogenetic analyses of 16S rRNA gene, atpD and recA sequences, these strains belong to the genus Rhizobium, with Rhizobium etli and Rhizobium leguminosarum as the closest related species. CCBAU 33202(T), a representative of these novel isolates, showed sequence similarity to its closest relatives R. etli CFN 42(T) and R. leguminosarum USDA 2370(T) of 99.5 and 99.1 % for the 16S rRNA gene, 91.9 and 91.9 % for atpD and 90.3 and 93.2 % for recA. The strains from this study could also be differentiated from R. etli CFN 42(T) and R. leguminosarum USDA 2370(T) by 16S-IGS RFLP and SDS-PAGE of whole-cell proteins, fatty acid profiles and several phenotypic characteristics. DNA-DNA hybridization yielded relatedness of 19 and 14-43 %, respectively, with R. etli CFN 42(T) and strains representing different biovars of R. leguminosarum. All data obtained in this study showed that these V. faba isolates belong to a novel species, for which the name Rhizobium fabae sp. nov. is proposed. The type strain, CCBAU 33202(T) (=LMG 23997(T) =JCM 14381(T)), was isolated from Nanchang.
authors.affiliation: Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Chang Fu
authors.initials: CF
authors.lastname: Tian
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
authors.forename: Li Juan
authors.initials: LJ
authors.lastname: Wu
authors.forename: Tian Xu
authors.initials: TX
authors.lastname: Han
authors.forename: Wen Feng
authors.initials: WF
authors.lastname: Chen
authors.forename: Chun Tao
authors.initials: CT
authors.lastname: Gu
authors.forename: Jin Gang
authors.initials: JG
authors.lastname: Gu
authors.forename: Wen Xin
authors.initials: WX
authors.lastname: Chen
mesh_terms: D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity; D031307:Vicia faba / Q000382:microbiology*
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S