Proteomics-based approach to elucidate the mechanism of antitumor effect of curcumin in cervical cancer.
Cervical cancer is the second leading cause of cancer death for women in the world. A potential target for preventing and treating cervical cancer is cyclooxygenase-2 (cox-2). Curcumin is an anti-inflammatory agent that is known to have anti-cox-2 activity. In this study we examined the expression of cox-2 in cervical cancer and its precursors by immunohistochemistry. The effect of curcumin in inhibiting cervical cancer cells was determined via 2-dimensional gel electrophoresis, data analysis, and ingenuity pathway analysis. No significant differences in the expression of cox-2 in squamous cell carcinoma, and carcinoma in situ were observed. However, there was a statistically significant difference in the expression of cox-2 in adenocarcinoma in comparison to normal (p value=0.01) and squamous cell carcinoma (p value=0.02) tissues. Proteins associated with cancer and cell cycle were significantly altered in cultured cells. Curcumin may have antitumor effect in cervical cancer.
Madden
Krystal
K
Department of Comparative Pathobiology, Purdue University, 725 Harrison Street, West Lafayette, IN 47907, USA.
Flowers
Lisa
L
Salani
Ritu
R
Horowitz
Ira
I
Logan
Sanjay
S
Kowalski
Kevin
K
Xie
Jun
J
Mohammed
Sulma I
SI
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053858:Metabolic Networks and Pathways; D040901:Proteomics; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D003474:Curcumin; D015232:Dinoprostone