The Escherichia coli K5 capsule is not synthesized in a protected compartment within the cytoplasm.
The intracellular expression of the K5 lyase enzyme, which degrades the K5 polysaccharide, decreased cell surface expression of the Escherichia coli K5 capsule. This indicates that biosynthesis of K5 polysaccharide in the cytoplasm is accessible to the action of K5 lyase and is not synthesized within a protected cytoplasmic compartment.
Hudson
Thomas
T
University of Manchester, United Kingdom.
Goldrick
Marie
M
Roberts
Ian S
IS
D016667:Bacterial Capsules / Q000096:biosynthesis*; D003090:Coliphages / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism / Q000821:virology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; C050673:capsular polysaccharide K5; C411971:KflA protein, E coli; D011133:Polysaccharide-Lyases