Secreted Frizzled-related protein 2 is a procollagen C proteinase enhancer with a role in fibrosis associated with myocardial infarction.
Secreted Frizzled-related proteins (sFRPs) have emerged as key regulators of a wide range of developmental and disease processes. Most of the known functions of mammalian sFRPs have been attributed to their ability to antagonize Wnt signalling. Recently however, Xenopus laevis and zebrafish sFRP, Sizzled, was shown to function as an antagonist of Chordin processing by Tolloid-like metalloproteinases. This has led to the proposal that sFRPs may function as evolutionarily conserved antagonists of chordinase activities of this class of proteinases. In contrast to this proposal, we show here that the mammalian sFRP, sFRP2, does not affect Chordin processing, but instead, can serve as a direct enhancer of procollagen C proteinase activity of Tolloid-like metalloproteinases. We also show that the level of fibrosis, in which procollagen processing by Tolloid-like proteinases has a rate-limiting role, is markedly reduced in Sfrp2-null mice subjected to myocardial infarction. Importantly, this reduced level of fibrosis is accompanied by significantly improved cardiac function. This study thus uncovers a function for sFRP2 and a potential therapeutic application for sFRP2 antagonism in controlling fibrosis in the infarcted heart.
Kobayashi
Koichi
K
Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY, USA.
Luo
Min
M
Zhang
Yue
Y
Wilkes
David C
DC
Ge
Gaoxiang
G
Grieskamp
Thomas
T
Yamada
Chikaomi
C
Liu
Ting-Chun
TC
Huang
Guorui
G
Basson
Craig T
CT
Kispert
Andreas
A
Greenspan
Daniel S
DS
Sato
Thomas N
TN
D000818:Animals; D055395:Bone Morphogenetic Protein 1 / Q000378:metabolism*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics; D005355:Fibrosis / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000235:genetics; D009203:Myocardial Infarction / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism; D020127:Recovery of Function / Q000502:physiology; D055394:Tolloid-Like Metalloproteinases / Q000378:metabolism; D000094742:Secreted Frizzled-Related Proteins
D055395:Bone Morphogenetic Protein 1; D003094:Collagen; D008565:Membrane Proteins; D055394:Tolloid-Like Metalloproteinases; C491628:Sfrp2 protein, mouse; D000094742:Secreted Frizzled-Related Proteins