title: Real-time polymerase chain reaction detection methods.
abstract: Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification. Real-time assay chemistries are subdivided into several main categories including DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer based systems. Specific advantages and applications of each category are highlighted herein.
authors.affiliation: Luminex Corporation, 12212 Technology Blvd., Austin, TX 78727, USA. dwhitman@luminexcorp.com
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Whitman
authors.forename: Sherry A
authors.initials: SA
authors.lastname: Dunbar
mesh_terms: D015342:DNA Probes / Q000235:genetics; D008954:Models, Biological; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D015342:DNA Probes