title: Presence of HPV type 6 in dysplasia and carcinoma arising from recurrent respiratory papillomatosis.
abstract: BACKGROUND
  We collected rare cases of recurrent respiratory papillomatosis (RRP) undergoing malignant transformation. We sought to identify human papillomavirus (HPV) subtypes in areas of papilloma, dysplasia, and carcinoma and investigate the pattern of protein overexpression.
  
  
  METHODS
  Three patients whose disease underwent malignant transformation from RRP to carcinoma were subjected to this study. Morphologically distinct areas in the pathology specimen of each patient were diagnosed as papilloma, dysplasia, and carcinoma. Each lesion was separately obtained by laser capture microdissection and was PCR amplified for the presence of HPV. A DNA chip was used to determine the type of HPV in each area. Immunohistochemistry for p53, Ki-67, and pRb was performed.
  
  
  RESULTS
  HPV type 6 was present in all specimens tested positive. Expression of p53 and Ki-67 increased with increasing severity of dysplastic change.
  
  
  CONCLUSIONS
  Although HPV type 11 is most frequently associated with malignant change of RRP, HPV type 6 may also contribute to play an equally important role in RRP carcinogenesis.
authors.affiliation: Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Jeong
authors.forename: Seok-Woo
authors.initials: SW
authors.lastname: Park
authors.forename: Miwha
authors.initials: M
authors.lastname: Shin
authors.forename: You Jeong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Jeon
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Jung
authors.forename: J Hun
authors.initials: JH
authors.lastname: Hah
authors.forename: Tack-Kyun
authors.initials: TK
authors.lastname: Kwon
authors.forename: Yong-Sang
authors.initials: YS
authors.lastname: Song
authors.forename: Kwang Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Myung-Whun
authors.initials: MW
authors.lastname: Sung
mesh_terms: D000328:Adult; D001707:Biopsy, Needle; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D018450:Disease Progression; D052163:Human papillomavirus 6 / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D010212:Papilloma / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000473:pathology*; D011379:Prognosis; D012189:Retrospective Studies; D018570:Risk Assessment; D012494:Sampling Studies