[Study on the gene polymorphism of Auberger antigens in Chinese population].
OBJECTIVE
To study the gene polymorphism of the Auberger antigens in Lutheran blood group system in Chinese population and establish a stable, accurate molecular method detecting Auberger antigens.


METHODS
Peripheral blood samples from 162 randomly collected and unrelated volunteer blood donors were directly sequenced for the exon 12 at the gene locus of Auberger antigens. PCR products with novel nucleotide were further investigated by restriction fragment length polymorphism (RFLP) analysis.


RESULTS
Auberger genotypes in the 162 Chinese individuals were obtained: Au(a+ b- )(nt1615A) was found in 119 individuals, Au(a+ b+ ) (nt1615A/G) in 40 individuals and Au(a- b+ ) (nt1615G) in 3 individuals. The allele frequencies of the Au(a) and Au(b) were 0.8580 and 0.1420, respectively. An individual with homozygous Au(a) genotype had a nucleotide mutation (1595 G to T). The mutation was confirmed by digesting the DNA with Hha I.


CONCLUSIONS
The distribution of gene polymorphism of Auberger antigens in a Chinese population was investigated and obtained. And a molecular method determining the Auberger antigen was established. A novel Lutheran allele was deposited in GenBank (accession number EU260043).
Zeng
Jianqiang
J
Shenzhen Blood Center, Shenzhen Institute of Transfusion Medicine, Shenzhen, Guangdong 518035, P. R. China.
Deng
Zhihui
Z
Yang
Baocheng
B
Jiang
Xiangyue
X
Su
Yuqing
Y
Lu
Liang
L
Yu
Qiong
Q
D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008188:Lutheran Blood-Group System / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D008188:Lutheran Blood-Group System