Rapid cell mapping using nanoparticles and SERRS.
Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
Stokes
Robert J
RJ
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UKG1 1XL.
McKenzie
Fiona
F
McFarlane
Emma
E
Ricketts
Alastair
A
Tetley
Laurence
L
Faulds
Karen
K
Alexander
James
J
Graham
Duncan
D
D004396:Coloring Agents; D006046:Gold; D008264:Macrophages / Q000648:ultrastructure*; D053758:Nanoparticles; D012680:Sensitivity and Specificity; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000379:methods
D004396:Coloring Agents; D012834:Silver; D006046:Gold