Engineering of hetero-functional gold nanorods for the in vivo molecular targeting of breast cancer cells.
A novel technique is described to functionalize gold nanorods (GNRs) allowing for in vivo targeting of breast cancer tumors grown in athymic nude mice. GNRs were functionalized by covalent attachment of Herceptin (HER), a monoclonal antibody that enables molecular recognition of breast cancer cells expressing highly specific tumor associated antigens, and poly(ethylene glycol) (PEG) which obscures particles against the reticuloendothelial system in the body. The stability and functionality of fabricated particles (Her-PEG GNRs) were demonstrated in vitro in the presence of blood and then in vivo in nude mice model for breast cancer. The results demonstrate successful tumor accumulation of functionalized gold nanorods within HER2/neu overexpressing breast tumors in tumor-bearing nude mice and support the notions that GNRs can be used for molecular imaging of tumor.
Eghtedari
Mohammad
M
Center for Biomedical Engineering, The University of Texas Medical Branch in Galveston, 301 University Boulevard, UTMB Route 0456, Galveston, Texas 77555, USA.
Liopo
Anton V
AV
Copland
John A
JA
Oraevsky
Alexander A
AA
Motamedi
Massoud
M
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D016503:Drug Delivery Systems / Q000379:methods*; D006046:Gold; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D015336:Molecular Probe Techniques; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000648:ultrastructure
D006046:Gold