[Extraction of praeruptorin A from Radix Peucedani by supercritical fluid extraction-CO2].
OBJECTIVE
To extract praeruptorin A from Radix Peucedani by supercritical fluid extraction (SFE)-CO2.


METHODS
After preliminary experiment, three main factors were acquired that could influence the result of SFE-CO2, including the time, pressure and temperature of the extraction. The optimal extraction process was carried out on orthogonal design, and SFE-CO2 was compared with the traditional methods.


RESULTS
In the extraction of the praeruptorin A, the best extraction conditions were 60 degrees C, 20 MPa, and duration for three hours. As cosolvent alcohol was added, the amount of extraction of the praeruptorin A increased, and the amount of SFE-CO2 extraction was higher than those of decoction and heating reflux.


CONCLUSIONS
The SFE-CO2 in extracting praeruptorin A from Radix Peucedani is feasible and reliable.
Liu
Ai-dong
AD
Department of Cardiology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province 130021, China.
Hou
Wei
W
Chen
Xue-song
XS
Qiu
Zhi-dong
ZD
D019661:Apiaceae / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry*
D003374:Coumarins; C441070:praeruptorin A