title: Approaches to the compositional analysis of DNA.
abstract: DNA base compositional analysis is something which is rarely undertaken today, but it is still a useful criterion for phage taxonomy. A variety of techniques are described including hydrolysis of the DNA to the level of bases or nucleosides and separation by paper chromatography or HPLC. Spectroscopic and spectrofluorometric procedures are also outlined.
authors.affiliation: Department of Chemistry, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Manderville
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D009705:Nucleosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D009705:Nucleosides