Genetic differentiation and phylogeny relationships of functional ApoVLDL-II gene in red jungle fowl and domestic chicken populations.
A total of 243 individuals from Red Jungle Fowl (Gallus gallus spadiceus), Rugao, Anka, Wenchang and Silikes chicken populations were used for polymorphism analysis in functional apoVLDL-II gene by Restriction fragment length polymorphism and single strand conformation polymorphism markers. The results show that Anka population has highest gene diversity and Shannon information index, while Red jungle fowl shows highest effective number of allele. In addition, the higher coefficient of genetic differentiation (Gst) across all loci in apoVLDL-II was indicating that high variation is proportioned among populations. As expected total gene diversity (Ht) has upper estimate compared with within population genetic diversity (Hs) across all loci. The mean Gst value across all loci was (0.194) indicating about 19.4% of total genetic variation could be explained by breeds differences, while the remaining 80.6% was accounted for differences among individuals. The average apoVLDL-II gene flow across all loci in five chicken populations was 1.189. The estimates of genetic identity and distance confirm that these genes are significantly different between genetically fat and lean population, because fat type breed Anka shows highest distance with the other Silikes and Rugao whish are genetically lean. In addition, Wenchang and Red jungle fowl were found more closely and genetically related than the other breeds with 49.4% bootstrapping percentages, then they were related to Silikes by 100% bootstrapping percentages followed by Rugao and finally all of them are related with exotic fat breed Anka.
Musa
Hassan H
HH
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Cheng
Jin H
JH
Bao
Wen B
WB
Li
Bi C
BC
Mekki
Dafaalla M
DM
Chen
Guo H
GH
D000483:Alleles; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001823:Body Composition / Q000235:genetics; D002645:Chickens / Q000145:classification / Q000235:genetics*; D051456:Gene Flow; D005819:Genetic Markers; D008079:Lipoproteins, VLDL / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic
D001053:Apolipoproteins; D005819:Genetic Markers; D008079:Lipoproteins, VLDL; C027610:apolipoprotein VLDL II