Protein microarray analysis of apoptosis-related protein expression following heat shock in human tongue squamous cell carcinomas containing different p53 phenotypes.
OBJECTIVE
Hyperthermia is useful in the treatment of human head and neck cancers, because it is relatively easy to regulate temperatures when compared to tumors located in deep organs. In this study, attention was focused on p53 as a possible predictive indicator for the efficacy of hyperthermic cancer therapy.


METHODS
Two kinds of cell lines were used. These were derived from a human squamous cell carcinoma (SAS) and had identical genetic backgrounds except for their p53 gene status. It was previously reported that the heat sensitivity and frequency of apoptosis in wild-type p53 cells (SAS/neo) were clearly elevated when compared with mutated p53 cells (SAS/mp53). In order to study the expression of apoptosis related proteins after heat treatment, protein microarray analysis was used.


RESULTS
The expression of apoptosis inhibitory proteins such as Bcl-2, Bcl-xL, NF-kappaB, COX2, STAT3, IL-6, and IKKalpha/1 was seen to increase after heat treatment in SAS/mp53 cells, but not in SAS/neo cells.


CONCLUSIONS
The result of these observations indicates that apoptosis inhibitory proteins (such as Bcl-2, Bcl-xL, IL-6, etc.) were highly induced in SAS/mp53 cells after heat treatment when compared to control SAS/neo cells.
Kajihara
Atsuhisa
A
Department of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University, Nara, Japan.
Takahashi
Akihisa
A
Ohnishi
Ken
K
Imai
Yuichiro
Y
Yamakawa
Nobuhiro
N
Yasumoto
Jun-ichi
J
Ohnishi
Takeo
T
Kirita
Tadaaki
T
D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000503:physiopathology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D018869:Heat-Shock Response / Q000502:physiology*; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D015398:Signal Transduction; D014062:Tongue Neoplasms / Q000235:genetics* / Q000503:physiopathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D016159:Tumor Suppressor Protein p53