Identification and characterization of structural proteins of orchid fleck virus.
Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions.
Kondo
Hideki
H
Research Institute for Bioresources, Okayama University, Kurashiki, Japan. hkondo@rib.okayama-u.ac.jp
Maeda
Takanori
T
Tamada
Tetsuo
T
D002586:Cesium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000032:analysis; D012209:Rhabdoviridae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology; D015678:Viral Structural Proteins / Q000737:chemistry*; D014771:Virion / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D002712:Chlorides; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D002586:Cesium; D012965:Sodium Chloride; C028019:cesium chloride