Mitochondrial dysfunction leads to reduced chronological lifespan and increased apoptosis in yeast.
We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological aging as compared to WT. Expression of genes encoding subunits of mitochondrial electron transport chain and ATP synthase is significantly downregulated in HsTnII, and as a consequence, HsTnII is not able to respire ethanol. All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis.
Aerts
An M
AM
Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium.
Zabrocki
Piotr
P
Govaert
Gilmer
G
Mathys
Janick
J
Carmona-Gutierrez
Didac
D
Madeo
Frank
F
Winderickx
Joris
J
Cammue
Bruno P A
BP
Thevissen
Karin
K
D017209:Apoptosis / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D004251:DNA Transposable Elements; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D017346:Protein Serine-Threonine Kinases; C530964:SCH9 protein, S cerevisiae