title: [T lymphocyte subgroups and serum levels of trace elements in patients with diarrhea type of irritable bowel syndrome].
abstract: To investigate the T lymphocyte subgroups and the levels of serum zinc (Zn), selenium (Se), iron (Fe), copper (Cu) in patients with diarrhea type of irritable bowel syndrome (D-IBS).
  
  
  
  A total of 30 D-IBS patients and 30 control subjects were enrolled in this study, and their peripheral blood samples were collected. The percentage of peripheral CD3, CD4, CD8 T lymphocytes were analyzed by flow cytometry, and the ratio of CD4/CD8 was calculated. Serum Zn, Fe and Cu levels were determined by atomic absorption spectrometry(AAS), and the Se level by atomic fluorometry.
  
  
  
  Compared with control group,the percentage of CD4 T lymphocyte and the ratio of CD4/CD8 in D-IBS group were significantly lower (P<0.01). However, there was no significant difference in serum Zn, Se, Fe, Cu levels between two groups (P>0.05).
  
  
  
  The declines of peripheral blood CD4 T lymphocytes and the ratio of CD4/CD8 may suggest a cellular immune abnormality in D-IBS patients. There was no significant difference in trace elements levels between the two groups.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, College of Medical, Zhejiang University, Hangzhou 31003, China.
authors.forename: Hang-bin
authors.initials: HB
authors.lastname: Jin
authors.forename: Zhu-ying
authors.initials: ZY
authors.lastname: Gu
authors.forename: Hong-wen
authors.initials: HW
authors.lastname: Zhao
authors.forename: Qun-yan
authors.initials: QY
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: You-ming
authors.initials: YM
authors.lastname: Li
mesh_terms: D000328:Adult; D016516:CD4-CD8 Ratio; D016022:Case-Control Studies; D003300:Copper / Q000097:blood; D003967:Diarrhea / Q000209:etiology; D005260:Female; D006801:Humans; D007501:Iron / Q000097:blood; D043183:Irritable Bowel Syndrome / Q000097:blood* / Q000150:complications / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012643:Selenium / Q000097:blood; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014131:Trace Elements / Q000097:blood*; D015032:Zinc / Q000097:blood
chemical_list: D014131:Trace Elements; D003300:Copper; D007501:Iron; D012643:Selenium; D015032:Zinc