title: Polypeptide from Chlamys farreri modulates UVB-induced activation of NF-kappaB signaling pathway and protection HaCaT cells from apoptosis.
abstract: Polypeptide from Chlamys farreri (PCF) possesses strong antioxidant and photochemo-preventive properties. Our previous study has preliminarily demonstrated that PCF could reduce the intracellular reactive oxygen species (ROS) production and protect UVB-induced HaCaT cells apoptosis. But the anti-apoptotic effects of PCF on components of cell signaling pathways leading to gene expression has not been clearly established. In this study we determined whether PCF affords protection of HaCaT cells against UVB-mediated activation of nuclear factor kappa B signal pathway, which is involved in apoptosis. The result showed that pretreatment of UVB-induced HaCaT cells with PCF, ROS scavenger NAC and NF-kappaB inhibitor MG132 effectively suppressed the apoptosis of HaCaT cells. PCF inhibited UVB-induced activation and translocation of NF-kappaB/p65 to nucleus, which was mediated through inhibition of phosphorylation/degradation and decreasing mRNA expression of IkappaBalpha and also blocking activation of IKKalpha in a dose-dependent manner. Furthermore, we observed that NAC also inhibited UVB-induced activation of NF-kappaB/p65 through decreasing the degradation and phosphorylation of IkappaBalpha. We concluded that the activation of NF-kappaB signal pathway played an important role in UVB-induced apoptosis, and PCF likely exerted its anti-apoptotic effect in HaCaT cells through decreasing intracellular ROS level and modulating the NF-kappaB signaling pathway.
authors.affiliation: Department of Pharmacology, Medical College of Qingdao University, Qingdao 266071, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Liu
authors.forename: Zhengyang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunbo
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D049897:Pectinidae / Q000737:chemistry*; D010455:Peptides / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology / Q000528:radiation effects*; D051996:Transcription Factor RelA / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D010455:Peptides; D017382:Reactive Oxygen Species; D051996:Transcription Factor RelA; D000072000:NF-KappaB Inhibitor alpha