[Effect of Atrazine on the content of calcium and expression of calmodulin in splenocytes of mice].
OBJECTIVE
To investigate the effects of Atrazine on the concentration of intracellular calcium ([Ca2+]i) and the expression of calmodulin (CaM) in splenocytes.


METHODS
The mice were divided into 5 groups, and were lavaged the doses of 100, 200 and 400 mg/kg Atrazine respectively. The mice were administrated Atrazine for 3 weeks. The Ca2+ concentrations in serum were detected by atomic absorption spectrophotometer. The [Ca2+]i in splenocytes were determined by fluorospectrophotometer. The expressions of CaM were detected by western blot analysis.


RESULTS
The changes of the Ca2+ concentrations of serum in treatment groups were not observed. The intracellular calcium concentrations and CaM expressions in treatment groups were significantly increased and decreased compared with the control group.


CONCLUSIONS
Atrazine could induced the increase of calcium concentration and the depression of the expression of CaM in splenocytes of mice.
Zhang
Yang
Y
School of Public Health, Harbin Medical University, Harbin 150086,China. zhangyang1962@sina.com
Wang
Mingqiu
M
Ren
Rui
R
Zhang
Xiaofeng
X
D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D006540:Herbicides / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D002147:Calmodulin; D004785:Environmental Pollutants; D006540:Herbicides; D001280:Atrazine; D002118:Calcium