Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori.
Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
Karasaki
Noriko
N
Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Mon
Hiroaki
H
Takahashi
Masateru
M
Lee
Jae Man
JM
Koga
Katsumi
K
Kawaguchi
Yutaka
Y
Kusakabe
Takahiro
T
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D012831:Bombyx; D004318:Doxycycline / Q000378:metabolism*; D015870:Gene Expression; D008967:Molecular Biology / Q000379:methods*; D017924:Nucleopolyhedroviruses / Q000235:genetics; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics; D015533:Transcriptional Activation
D000952:Antigens, Polyomavirus Transforming; D004318:Doxycycline