title: Detection of specifically oxidized apolipoproteins in oxidized HDL.
abstract: Atherosclerosis is associated with dysfunctional HDL, and oxidation of HDL is thought to give rise to HDL becoming dysfunctional. Lipoprotein oxidation represents a complex series of processes that can be assessed by various methods. In general, oxidation mediated by 1-electron or radical oxidants gives rise to lipid hydroperoxides (LOOHs) as the primary product. These LOOHs may then undergo further reactions giving rise to secondary lipid oxidation products and/or oxidation of lipoprotein-associated proteins. Thus, LOOHs specifically oxidize Met residues of apolipoprotein (apo) A-I and A-II (the major proteins of HDL) to MetO. Here we describe an HPLC-based method to detect oxidized HDL containing specifically oxidized forms of apoA-I and apoA-II. This method may be useful to assess the early stages of HDL oxidation in biological samples.
authors.affiliation: Centre for Vascular Research, Bosch Institute and Discipline of Pathology, The University of Sydney, Sydney, Australia.
authors.forename: Xiao Suo
authors.initials: XS
authors.lastname: Wang
authors.forename: Roland
authors.initials: R
authors.lastname: Stocker
mesh_terms: D000578:Amidines / Q000494:pharmacology; D016632:Apolipoprotein A-I / Q000097:blood / Q000737:chemistry; D016633:Apolipoprotein A-II / Q000097:blood / Q000737:chemistry; D001053:Apolipoproteins / Q000097:blood* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005838:Genotype; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D052250:Nitric Oxide Synthase Type III / Q000235:genetics; D010084:Oxidation-Reduction / Q000187:drug effects; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000578:Amidines; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D001053:Apolipoproteins; D008075:Lipoproteins, HDL; C046728:2,2'-azobis(2-amidinopropane); D052250:Nitric Oxide Synthase Type III