title: Epithelial cells within the human pancreas do not coexpress mesenchymal antigens: epithelial-mesenchymal transition is an artifact of cell culture.
abstract: Pancreatic mesenchymal stem cells (MSCs) may be derived from human beta-cells undergoing reversible epithelial-mesenchymal transition (EMT), suggesting that they could be a potential source of new beta-cells. In this study we sought to determine the origin of pancreatic MSCs in the nonendocrine pancreas. Double immunofluorescent (IF) staining and flow cytometry were used to assess the cell phenotype of nonendocrine pancreas tissue following islet procurement, during in vitro expansion of MSCs, and after differentiation. IF staining of paraffin-embedded pancreatic biopsy sections was used to assess cell phenotype in vivo. In this study we demonstrated that: (1) pancreatic epithelial cells do not express MSC antigens in vivo; (2) following islet isolation EpCAM- and CK19-positive epithelial cells coexpressed the MSC antigens CD44 (32+/-8% and 38+/-10%) and CD29 (85+/-4% and 64+/-4%); (3) during in vitro expansion the number of single-positive epithelial and double-positive epithelial/MSCs decreased whereas the number of single-positive MSCs increased and (4) differentiated MSCs do not revert to a true epithelial cell phenotype in our culture conditions, as epithelial cell surface markers (EpCAM, CK19 and E-Cadherin) are not reexpressed, although the MSC phenotype is altered. This study demonstrates that MSCs may be derived in vitro via a pancreatic epithelial cell undergoing EMT, however it is more likely that a small percentage of MSCs that reside in the adult pancreas are proliferating whereas the epithelial cells are negatively selected by the experimental culture conditions.
authors.affiliation: Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Seeberger
authors.forename: Alana
authors.initials: A
authors.lastname: Eshpeter
authors.forename: Ray V
authors.initials: RV
authors.lastname: Rajotte
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Korbutt
mesh_terms: D000328:Adult; D000368:Aged; D016477:Artifacts; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D016130:Immunophenotyping; D019012:Integrin beta1 / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D046790:Pancreas, Exocrine / Q000166:cytology*; D010641:Phenotype
chemical_list: D015415:Biomarkers; D018960:Hyaluronan Receptors; D019012:Integrin beta1