title: Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system.
abstract: Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.
authors.forename: Kwang-Wook
authors.initials: KW
authors.lastname: Ko
authors.forename: Fengqiu
authors.initials: F
authors.lastname: Lin
authors.forename: Takumi
authors.initials: T
authors.lastname: Katsumata
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sugai
authors.forename: Sho
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaide
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
mesh_terms: D000595:Amino Acid Sequence; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression; D008861:Microsomes / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D012275:Oryza / Q000201:enzymology*; D010843:Pichia / Q000166:cytology / Q000235:genetics*
chemical_list: D003577:Cytochrome P-450 Enzyme System; C066398:ent-kaurene oxidase; D009251:NADPH-Ferrihemoprotein Reductase