title: Establishment and characterization of HER2-positive cell line derived from pleural effusion of human breast scirrhous carcinoma.
abstract: We have established a cell line derived from a pleural effusion of breast scirrhous carcinoma. This cell line presented immunohistochemically negative for estrogen receptor and slightly positive for progesterone receptor, and positive for c-erbB/HER2/neu. However the original tumor was found to be positive for estrogen receptor and progesterone receptor and slightly positive for c-erbB/HER2/neu expression. Enzyme and electrochemiluminescence immunoassays of tumor markers in the conditioned medium by the cell line revealed they secrete CA15-3, NCC-ST-439 and HER2 protein. We believe the cell line will contribute to the therapeutic study of malignant breast cancer.
authors.affiliation: Department of Surgery, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Tabei
authors.forename: Shigeki
authors.initials: S
authors.lastname: Nishiyama
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamashita
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Ken
authors.initials: K
authors.lastname: Uchida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D002293:Adenocarcinoma, Scirrhous / Q000235:genetics / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis; D009368:Neoplasm Transplantation; D016066:Pleural Effusion, Malignant / Q000166:cytology / Q000235:genetics / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D018396:Mucin-1; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C056315:ST 439 antigen, human