Apoptotic activity of a nuclear form of mitogaligin, a cell death protein.
Galig, an internal gene to the galectin-3 gene, encodes two proteins and induces cell death in human cells. Mitogaligin, one of these proteins, contains a mitochondrial targeting sequence and promotes the release of cytochrome c into the cytosol. Here, we show that mitogaligin can also localize to nucleus. The nuclear form of mitogaligin induced cell death through a pathway exhibiting typical properties of apoptosis. These observations indicate for the first time that mitogaligin expresses cytotoxic properties not only when addressed to mitochondria but also when targeted to the nucleus.
Gonzalez
Patrick
P
Centre de Biophysique Moléculaire, CNRS UPR4301, Orléans Cedex 2, France.
Robinet
Pauline
P
Charpentier
Stéphane
S
Mollet
Lucile
L
Normand
Thierry
T
Dubois
Martine
M
Legrand
Alain
A
D017209:Apoptosis; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004249:DNA Damage; D037161:Galectins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D021381:Protein Transport; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D001798:Blood Proteins; D037161:Galectins; C428445:LGALS3 protein, human; D051028:bcl-2-Associated X Protein; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins