title: HSP70 and mucin 5B: novel protein targets of N,N'-dinitrosopiperazine-induced nasopharyngeal tumorigenesis.
abstract: N,N'-Dinitrosopiperazine (DNP) induces nasopharyngeal carcinoma (NPC) and shows organ specificity to the nasopharyngeal epithelium. To investigate its mechanism, the rat NPC model was induced using DNP. Rat NPC and normal nasopharyngeal cells were obtained from the NPC model using laser capture. The total proteins from these cell samples were separated with two-dimension polyacrylamide gel electrophoresis techniques, and highly expressed proteins (> five-fold) were analyzed using matrix-assisted laser desorption/ionization time of flight and bioinformatics. The results showed that HSP70 and mucin 5B expression increased not only in rat NPC but also in atypical hyperplasia nasopharyngeal tissues, a precancer stage of NPC. High-expression of heat shock protein 70 (HSP70) and mucin 5B was further supported by western blot analysis. The immunofluorescence and western-blotting studies further showed that DNP induced the expression of HSP70 and mucin 5B in a dosage-dependent manner in normal nasopharyngeal epithelia cells. Our data indicate that DNP triggers over-expression of HSP70 and mucin 5B, and is involved in nasopharyngeal tumorigenesis. HSP70 and mucin 5B may be important targets in nasopharyngeal tumorigenesis induced by DNP.
authors.affiliation: Xiangya Hospital, Department of Pathology, Xiangya School of Medicine, Central South University,Changsha, Hunan, China. tangfaqing333@hotmail.com
authors.forename: Fa-Qing
authors.initials: FQ
authors.lastname: Tang
authors.forename: Chao-Jun
authors.initials: CJ
authors.lastname: Duan
authors.forename: Da-Mao
authors.initials: DM
authors.lastname: Huang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Wang
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Xie
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Meng
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Jiang
authors.forename: De-Yun
authors.initials: DY
authors.lastname: Feng
authors.forename: Shang-Hui
authors.initials: SH
authors.lastname: Wu
authors.forename: Huan-Hua
authors.initials: HH
authors.lastname: Gu
authors.forename: Mo-Yu
authors.initials: MY
authors.lastname: Li
authors.forename: Fu-Liang
authors.initials: FL
authors.lastname: Deng
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Gong
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Yong-Hao
authors.initials: YH
authors.lastname: Xu
authors.forename: Can
authors.initials: C
authors.lastname: Tan
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens / Q000633:toxicity*; D049109:Cell Proliferation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D055257:Mucin-5B / Q000378:metabolism*; D009303:Nasopharyngeal Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009602:Nitrosamines / Q000633:toxicity; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002273:Carcinogens; D018840:HSP70 Heat-Shock Proteins; D055257:Mucin-5B; D009602:Nitrosamines; C026156:N,N'-dinitrosopiperazine