title: Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses.
abstract: A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Ngan Thi Kim
authors.initials: NT
authors.lastname: Pham
authors.forename: Quang Duy
authors.initials: QD
authors.lastname: Trinh
authors.forename: Tuan Anh
authors.initials: TA
authors.lastname: Nguyen
authors.forename: Shuvra Kanti
authors.initials: SK
authors.lastname: Dey
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.forename: Le Phuc
authors.initials: le P
authors.lastname: Hoang
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D016679:Genome, Viral; D005838:Genotype; D052699:Kobuvirus / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D012367:RNA, Viral