Towards a pragmatic strategy for regenerating infarcted myocardium with glandular stem cells.
We have recently reported that the in vitro differentiation of human glandular stem cells into cardiac-like cells can be enhanced by co-culture with small myocardial biopsies. These results suggest that implantation of such cells directly into infarcted myocardium may facilitate the regeneration of the heart. As a preliminary to testing this approach in a goat model, pilot in vitro tests for these experiments have been performed and are presented here. Stem cells, isolated from the glandula submandibularis of Boer goats (SuSCs), have been co-cultured either directly or indirectly with heart biopsies from various species (Boer goat, rattus norwegicus, human) or heart conditioned medium for 48h. We found a substantial increase in the number of cells expressing heart-specific marker proteins (Troponin I, Troponin T, sarcomeric myosin) regardless of the source organism of the heart biopsy. The proliferation of SuSCs also increased significantly under co-culture conditions. To benefit from these results in vivo, the stem cells must be delivered to the infarcted region in the heart and held securely in place over lengthy periods of time. Therefore, we repeated the co-culture experiments with SuSCs grown on biodegradable Vicryl-meshes. The cells demonstrated good proliferation on the meshes and likewise, the expression of heart-specific marker proteins could be enhanced through co-culture with heart biopsies.
Maass
Antje
A
Fraunhofer Institute of Marine Biotechnology, MGZ, Paul-Ehrlich-Strasse 1-3, D-23562 Luebeck, Germany.
Kajahn
Jennifer
J
Guerleyik
Emel
E
Guldner
Norbert W
NW
Rapoport
Daniel H
DH
Kruse
Charli
C
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D018920:Coculture Techniques / Q000379:methods; D017077:Culture Media, Conditioned; D004195:Disease Models, Animal; D006041:Goats; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology* / Q000601:surgery*; D010865:Pilot Projects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D012469:Salivary Glands / Q000166:cytology* / Q000502:physiology; D013045:Species Specificity; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D013363:Submandibular Gland / Q000166:cytology* / Q000502:physiology
D017077:Culture Media, Conditioned