Isolation of Phage via Induction of Lysogens.
Most bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using Mitomycin C, and for the isolation of prophage-cured bacterial cell lines.
Raya
Ra'l R
RR
Cerela-Conicet, S.M. de Tucumá, Tucumá, Argentina.
H'bert
Elvira M
EM
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D008242:Lysogeny / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D016685:Mitomycin / Q000494:pharmacology; D039002:Prophages / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin