title: An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in Arabidopsis.
abstract: Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca(2+)-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.
authors.affiliation: Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Ho Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Mi Soon
authors.initials: MS
authors.lastname: Jung
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Lee
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Kim
authors.forename: Jae Hyuk
authors.initials: JH
authors.lastname: Yoo
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Kim
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Moo Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D029681:Arabidopsis Proteins; D002147:Calmodulin; C538996:CBRLK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases