RNA-protein interaction mediating post-transcriptional regulation in the circadian system.
Post-transcriptional control makes an important contribution to shaping transcript profiles of circadianly regulated genes. In Arabidopsis thaliana, the clock-regulated glycine-rich RNA-binding protein ATGRP7 oscillates with a 24-h rhythm and transmits the rhythmicity generated by the central oscillator within the cell. ATGRP7 negatively auto-regulates its own expression at the post-transcriptional level. In response to an elevated protein level, a shift to a cryptic 5' splice site within the intron occurs, leading to an unproductively spliced transcript that rapidly vanishes due to its short half-life. This feedback regulation relies on direct binding of the RNA-binding protein to its own RNA. Here we describe the analysis of RNA-protein interaction in vitro employing recombinant RNA-binding protein and 32P-labelled in vitro transcripts or synthetic RNA oligoribonucleotides comprising the binding site under study.
Schöning
Jan C
JC
Department of Molecular Cell Physiology, University of Bielefeld, Bielefeld, Germany.
Staiger
Dorothee
D
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D008957:Models, Genetic; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; C118496:GRP7 protein, Arabidopsis