title: Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system.
abstract: Cellular internalization of functional nanoparticles that have optical and magnetic properties is very important in the cellular imaging and manipulation of specifically targeted biomolecules. In this study, a robust method to deliver functional nanoparticles and targeting antibodies into cells was suggested. The engineered protein G system, which contains an affinity tag and a cell penetration peptide in the N- and C-terminals, respectively, can capture surface-modified nanoparticles and antibodies without chemical reaction, and then non-invasively deliver them into the cells. Finally, gold-coated iron oxide nanoparticle/engineered protein G hybrid systems were successfully employed as multifunctional cargo systems for the targeting, imaging, and manipulation of mitochondria.
authors.affiliation: Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-806, South Korea.
authors.forename: Yong Taik
authors.initials: YT
authors.lastname: Lim
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Cho
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Sang Jeon
authors.initials: SJ
authors.lastname: Chung
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D000906:Antibodies / Q000494:pharmacology*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects* / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering; D045663:Quantum Dots; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D000906:Antibodies; C029152:G-substrate; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins