[The inhibition of oxidative and nitrosative stresses by ecdysterone as the mechanisms of its cardio- and vasoprotective action in experimental diabetes type I].
Streptozotocine (STZ) administration (5 mg/100 g) up regulates oxidative (lipid peroxidation as a marker) and nitrosative (protein nitrosilation as a marker) stresses as well as ROS (O(2-), H2O2, OH) generation in heart and aorta in rats after 60 days of STZ action. The level of oxydative stress was higher in aorta. Xanthine oxidase (XO) activation (uric acid as marker), but not lipoxygenase (LTC4 as marker) or cyclooxygenase (TxB2 as marker) are the main oxydases that generate O(2-) as calculated by correlation analysis. STZ administration led to sphingosine pools up regulation in heart and aorta, but pools of polyamines in this organ was down regulated. C27-phytosteroid hormone ecdysterone (100 ng/100 g, per os, 60 days) mimics the action of its structural analog C27-steroid hormone calcitriol (1alpha,25-dihydroxyvitamin D,) and protects rise of ROS generation (by XO inhibition), lipid peroxidation, protein nitrosilation, polyamine degradation in heart and aorta of rats after STZ administration. The new mechanism of iNOS activation, prostaglandine and tetrahydrobiopterin synthesis stimulation by ecdysterone has been proposed. It was due to stimulating enzymatic degradation of sphingosine-1-phosphate as effective regulator of iNOS, COX and GTP-cyclohydrolase in cardio-vascular system: sphingomyelin > ceramide > sphingosine > S-I-P > phosphoethanolamine > ethanolamine.
Sahach
V F
VF
Korkach
Iu P
IuP
Kotsiuruba
A V
AV
Prysiazhna
O D
OD
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D002319:Cardiovascular System / Q000187:drug effects* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D004441:Ecdysterone / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D015538:Nitrosation / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D011921:Rats, Inbred WKY; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D004441:Ecdysterone