Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses.
A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies.
Pham
Ngan Thi Kim
NT
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Trinh
Quang Duy
QD
Nguyen
Tuan Anh
TA
Dey
Shuvra Kanti
SK
Phan
Tung Gia
TG
Hoang
Le Phuc
le P
Khamrin
Pattara
P
Maneekarn
Niwat
N
Okitsu
Shoko
S
Mizuguchi
Masashi
M
Ushijima
Hiroshi
H
D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D016679:Genome, Viral; D005838:Genotype; D052699:Kobuvirus / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D036022:Capsid Proteins; D017931:DNA Primers; D012367:RNA, Viral