Surface modification by 2-methacryloyloxyethyl phosphorylcholine coupled to a photolabile linker for cell micropatterning.
This report describes a new surface-treatment technique for cell micropatterning. Cell attachment was selectively controlled on the glass surface using a photochemical reaction. This strategy is based on combining 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, which is known to reduce non-specific adsorption, and a photolabile linker (PL) for selective cell patterning. The MPC polymer was coated directly on the glass surface using a straightforward surface modification method, and was removed by ultraviolet (UV) light illumination. All the surface modification steps were evaluated using static water contact angle measurements, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), measurements of non-specific protein adsorption, and the cell attachment test. After selective cleavage of the MPC polymer through the photomask, cells attached only to the UV-illuminated region where the MPC polymer was removed, which made the hydrophilic surface relatively hydrophobic. Furthermore, the size of the MC-3T3 E1 cell patterns could be controlled by single cell level. Stability of the cell micropatterns was demonstrated by culturing MC-3T3 E1 cell patterns for 5 weeks on glass slide. The micropatterns were stable during culturing; cell viability also was verified. This method can be a powerful tool for cell patterning research.
Jang
Kihoon
K
Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan.
Sato
Kae
K
Mawatari
Kazuma
K
Konno
Tomohiro
T
Ishihara
Kazuhiko
K
Kitamori
Takehiko
T
D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000737:chemistry*; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011506:Proteins; D010767:Phosphorylcholine