[Detection of the SRY gene by primed in situ labeling].
OBJECTIVE
To establish a primed in situ labeling (PRINS) technique which can be more effective in detection of single copy gene.


METHODS
On the basis of traditional PRINS, new reagents and procedures, such as TaqStart antibody, four primers of the sex determining region Y (SRY) gene and TSA(TM) Biotin System were included in detection of the SRY gene. Meanwhile, fluorescence in situ hybridization(FISH) to detect the SRY gene was used as control.


RESULTS
Fifty metaphases were scored. PRINS labeling showed signals for the SRY on the Y chromosome at band Yp11.3 in all metaphases. These signals were as distinct as that from results of FISH.


CONCLUSIONS
This improved method is ideal for rapidly localizing single copy genes and small DNA segments. And PRINS is a cost- and time-effective alternative to FISH.
Nie
Yong
Y
Institute of Medical Genetics, Sichuan University School of Life Science, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, Sichuan 610064, P. R. China.
Ding
Xianping
X
Deng
Li
L
Wei
Ping
P
Wang
Huan
H
D018628:Gene Dosage; D024282:Genes, sry / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008677:Metaphase / Q000235:genetics; D020401:Primed In Situ Labeling / Q000379:methods*