title: Identification of endo- and exo-polygalacturonase activity in Lygus hesperus (Knight) salivary glands.
abstract: Polygalacturonase (PG) activity found in the salivary gland apparatus of the western tarnished plant bug (WTPB, Lygus hesperus Knight) has been thought to be the main chemical cause of the damage inflicted by this mirid when feeding on its plant hosts. Early viscosity and thermal stability studies of the PG activity in L. hesperus protein extracts were difficult to interpret. Thus, it has been suggested that one or more PG protein(s) with different hydrolytic modes of action are produced by this mirid. In order to understand the quantitative complexity of the WTPB salivary PG activity, PG purification from a protein extract from salivary glands excised from L. hesperus insects was performed using affinity and ion exchange chromatography. To elucidate the qualitative complexity of the purified PGs, the digestion products generated by the PGs were separated using high performance anion exchange chromatography with pulsed amperometric detection. At least five PG proteins were detected; these differing in terms of their glycosylation, mass-to-charge ratios, and/or molecular mass. The characterization of the products generated by these PGs showed that endo- and exo-acting PGs are produced by WTPB. Although none of the PGs was purified to homogeneity, the present work provides biochemical evidence of a multiplicity of PGs that degrade the pectin component of the plant tissue in different fashions. The implications of these findings affect the understanding of WTPB feeding damage and, potentially, help identify ways to control this important crop pest. Arch. Insect Biochem. Physiol. 2008. (c) 2008 Wiley-Liss, Inc.
authors.affiliation: Plant Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Maria de la Paz
authors.initials: Mde L
authors.lastname: Celorio-Mancera
authors.forename: L
authors.initials: L
authors.lastname: Carl Greve
authors.forename: Larry R
authors.initials: LR
authors.lastname: Teuber
authors.forename: John M
authors.initials: JM
authors.lastname: Labavitch
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005247:Feeding Behavior; D020062:Heteroptera / Q000201:enzymology*; D011096:Polygalacturonase / Q000302:isolation & purification / Q000378:metabolism*; D012469:Salivary Glands / Q000201:enzymology*
chemical_list: D011096:Polygalacturonase