title: Genetic relationships among wild Felidae in Thailand using AFLP markers.
abstract: The cytogenetics of eight Felidae species in Thailand were investigated by the colchicines-hypotonic fixation-air drying technique followed by a conventional technique. All species studied have an identical number of 38 diploid chromosomes, indicating a close genetic relationship among species. At a deep study level, the genetic relationships of eight Felidae species were accessed by the AFLP method. Blood samples were collected from sources locating in their original regions for DNA extraction. With ten successful primer combinations, a total of 4208 scorable bands were generated. Of these bands, 18.91% are polymorphic. Percentages of Polymorphic Bands (PPB) for each primer combination range from 15.00 to 23.59%. The generating bands were used for dendrogram construction. The average genetic similarity values among all Felidae species are 68.20% (between Panthera tigris and Neofelis nebulosa) to 85.53% (between Prionailurus bengalensis and Prionailurus viverrinus). The dendrogram shows that the eight Felidae species were clustered together and the subfamily Pantherinae and Felinae with Neofelis nebulosa are distinguished. The Felinae, Prionailurus bengalensis, Prionailurus viverrinus, Catopuma temminckii, Felis chaus, Pardofelis marmorata and Neofelis nebulosa were clustered together with 91% bootstrap support and the Pantherinae, Panthera pardus is clustered with Panthera tigris with 92% bootstrap support. In summary, the ten successful primer combinations can be used to determine genetic differences among eight Thailand Felidae species.
authors.affiliation: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Nattapong
authors.initials: N
authors.lastname: Srisamoot
authors.forename: Arunrat
authors.initials: A
authors.lastname: Chaveerach
authors.forename: Suporn
authors.initials: S
authors.lastname: Nuchadomrong
authors.forename: Nison
authors.initials: N
authors.lastname: Sattayasai
authors.forename: Prapansak
authors.initials: P
authors.lastname: Chaveerach
authors.forename: Alongkoad
authors.initials: A
authors.lastname: Tanomtong
authors.forename: Krit
authors.initials: K
authors.lastname: Pinthong
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D000835:Animals, Wild / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D016000:Cluster Analysis; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004171:Diploidy; D017753:Ecosystem; D045989:Felidae / Q000235:genetics*; D005819:Genetic Markers; D008214:Lymphocytes / Q000502:physiology; D011110:Polymorphism, Genetic; D013785:Thailand
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA