Amplification and cloning of herpes simplex virus type 2 glycoprotein G from an Iranian isolate.
Herpes Simplex Virus type-2 (HSV-2) is the main cause of genital herpes infection. Its prevalence is increasing worldwide and varies widely with generally higher rate in developing than developed countries and urban than rural areas. HSV-2 Iranian isolate was propagated in HeLa cell line. The viral genome was extracted by phenol-chloroform and used as template in nested polymerase chain reactions (n-PCR) to amplify gG-2 gene. The amplified gene was cloned into a cloning vector (pTZ57R/T) and transformed into competent E. coli DH5a. The recombinant vector encoding the gene of interest was confirmed by restriction enzyme analysis and sequencing.
Jamalidoost
Marzieh
M
Department of Virology, Faculty of Medical Science, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran.
Soleimanjahi
Hoorieh
H
Fotouhi
Fatemeh
F
Meshkat
Zahra
Z
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D018258:Herpesvirus 2, Human / Q000235:genetics*; D006801:Humans; D007492:Iran; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D017931:DNA Primers; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2