The FUSE binding proteins FBP1 and FBP3 are potential c-myc regulators in renal, but not in prostate and bladder cancer.
BACKGROUND
The three far-upstream element (FUSE) binding proteins (FBP1, FBP2, and FBP3) belong to an ancient family of single-stranded DNA binding proteins which are required for proper regulation of the c-myc proto-oncogene. Whereas it is known that c-myc alterations play a completely different role in various carcinomas of the urogenital tract, the relevance of FBPs is unclear.


METHODS
FBP1, FBP3 and c-myc expression was studied in 105 renal cell, 95 prostate and 112 urinary bladder carcinomas by immunohistochemistry using tissue microarrays.


RESULTS
High rates of FBP1 and FBP3 expression were observed in all cancer types. There was a concomitant up-regulation of FBP1 and FBP3 in renal cell and prostate carcinomas (p < 0.001 both). C-myc expression was detectable in 21% of prostate, 30% of renal and 34% of urothelial carcinomas. Interestingly, strong FBP1 and FBP3 expression was associated with c-myc up-regulation in clear cell renal cell carcinomas (p < 0.001 and 0.09 resp.), but not in bladder or prostate cancer.


CONCLUSIONS
The correlation between FBP1/FBP3, c-myc and high proliferation rate in renal cell carcinoma provides strong in vivo support for the suggested role of FBP1 and FBP3 as activators of c-myc. The frequent up-regulation of FBP1 and FBP3 in urothelial and prostate carcinoma suggests that FBPs also have an important function in gene regulation of these tumors.
Weber
Achim
A
Department of Pathology, Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland. achim.weber@usz.ch
Kristiansen
Ilka
I
Johannsen
Manfred
M
Oelrich
Beibei
B
Scholmann
Katharina
K
Gunia
Sven
S
May
Matthias
M
Meyer
Hellmuth-Alexander
HA
Behnke
Silvia
S
Moch
Holger
H
Kristiansen
Glen
G
D016009:Chi-Square Distribution; D004265:DNA Helicases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D000090063:Proto-Oncogene Mas; D016601:RNA-Binding Proteins; D018709:Statistics, Nonparametric; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology; D014565:Urogenital Neoplasms / Q000378:metabolism* / Q000473:pathology
D004268:DNA-Binding Proteins; C492258:FUBP1 protein, human; C492259:FUBP3 protein, human; C000715470:MAS1 protein, human; C116115:MYCBP protein, human; D000090063:Proto-Oncogene Mas; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D004265:DNA Helicases