Biosynthetic origins of the ionophore antibiotic indanomycin.
The pyrroloketoindane antibiotic indanomycin is produced by Streptomyces antibioticus NRRL 8167. These hybrid nonribosomal peptide-polyketide ionophore antibiotics are characterized by the presence of an unusual indane ring system, and there is interest in identifying the biochemical mechanisms guiding its biosynthesis. Following incorporation of [1-(13)C]-labeled precursors, the primary metabolic origins of indanomycin were determined to be one unit of L-proline, six units of malonyl-CoA and two units each of methylmalonyl-CoA and ethylmalonyl-CoA.
Roege
Kathryn E
KE
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Kelly
Wendy L
WL
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D007476:Ionophores / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011714:Pyrans / Q000737:chemistry / Q000378:metabolism*; D013303:Streptomyces antibioticus / Q000378:metabolism; D011758:Pyrroles
D000900:Anti-Bacterial Agents; D007476:Ionophores; D011714:Pyrans; C114124:indanomycin; D011758:Pyrroles