title: Diagnosis of human immunodeficiency virus type 1 infection in infants by use of dried blood spots and an ultrasensitive p24 antigen assay.
abstract: We tested 617 dried blood spots (DBS) from human immunodeficiency virus-exposed infants from five countries using an ultrasensitive p24 antigen assay (Up24). The sensitivity was 94.4% (67/71) and the specificity was 100% (431/431) for infants with DBS specimens <or=20 months old; DBS older than 30 months demonstrated only 72.2% sensitivity (39/54) (P < 0.001) but displayed 100% specificity (61/61).
authors.affiliation: Department Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7290, USA.
authors.forename: Ada
authors.initials: A
authors.lastname: Cachafeiro
authors.forename: Gayle G
authors.initials: GG
authors.lastname: Sherman
authors.forename: Annette H
authors.initials: AH
authors.lastname: Sohn
authors.forename: Consuelo
authors.initials: C
authors.lastname: Beck-Sague
authors.forename: Susan A
authors.initials: SA
authors.lastname: Fiscus
mesh_terms: D001769:Blood / Q000821:virology*; D003890:Desiccation; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
chemical_list: D016655:HIV Core Protein p24