title: Production of coronavirus nonstructural proteins in soluble form for crystallization.
abstract: For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.
authors.affiliation: Center for Structural and Cell Biology in Medicine, Institute of Biochemistry, University of Lübeck, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Piotrowski
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ponnusamy
authors.forename: Stephanie
authors.initials: S
authors.lastname: Glaser
authors.forename: Anniken
authors.initials: A
authors.lastname: Daabach
authors.forename: Ralf
authors.initials: R
authors.lastname: Moll
authors.forename: Rolf
authors.initials: R
authors.lastname: Hilgenfeld
mesh_terms: D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012995:Solubility; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C087633:nonstructural protein, coronavirus