[Comparative study of two primary culture methods of human nasal epithelial cells in vitro].
OBJECTIVE
To compare two primary culture methods of human nasal epithelial (HNE) in vitro, and explore a suitable method to be used in further study.


METHODS
Achievement ratio and growth curve of primarily cultural HNE by tissue piece culture were compared to those by isolated cell culture. Shape and appearance were observed and cellular sources were identified to get preliminary bionomics of HNE.


RESULTS
The isolated cell culture method (87.5%) was shown to be superior to tissue piece culture method (83.33%) by comparing achievement ratios, but no statistical significance was found (P > 0.05). The growth curve of isolated cell culture was higher than that of tissue piece culture. All cultured cells were confirmed coming from epithelial cells by observing shape, appearance and dyeing cytokeratin.


CONCLUSIONS
The isolated cell culture method is more suitable for primary culture due to its less promiscuity, faster proliferation, and more stable and reliable cell supply.
Wang
Zhenlin
Z
Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China. wzl1812@yahoo.com.cn
Zhang
Qiuhang
Q
Li
Yuan
Y
Li
Peng
P
Ye
Jin
J
Yang
Qintai
Q
Li
Yulu
Y
D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology*