title: Characterization of neutralizing epitopes of varicella-zoster virus glycoprotein H.
abstract: Varicella-zoster virus (VZV) glycoprotein H (gH) is the major neutralization target of VZV, and its neutralizing epitope is conformational. Ten neutralizing human monoclonal antibodies to gH were used to map the epitopes by immunohistochemical analysis and were categorized into seven epitope groups. The combinational neutralization efficacy of two epitope groups was not synergistic. Each epitope was partially or completely resistant to concanavalin A blocking of the glycomoiety of gH, and their antibodies inhibited the cell-to-cell spread of infection. The neutralization epitope comprised at least seven independent protein portions of gH that served as the target to inhibit cell-to-cell spread.
authors.affiliation: Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Akahori
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Tohru
authors.initials: T
authors.lastname: Daikoku
authors.forename: Masae
authors.initials: M
authors.lastname: Iwai
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Asano
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Shiraki
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D008562:Membrane Glycoproteins; D014764:Viral Proteins; C040496:gp 118 protein, Herpesvirus 3, Human