Molecular characterization of gonadotropin subunits and gonadotropin receptors in black porgy, Acanthopagrus schlegeli: effects of estradiol-17beta on mRNA expression profiles.
The cDNAs of three gonadotropin (GTH) subunits (GTHalpha, FSHbeta, and LHbeta) and two GTH receptors (FSHR and LHR) from pituitary and gonads of black porgy were cloned. The nucleotide sequences of the GTHalpha, FSHbeta, and LHbeta cDNA were 354, 363, and 414 base pairs (bps) in length with open reading frames (ORF) encoding peptides of 117, 120, and 137 amino acids, respectively. The FSHR and LHR cDNA was 2118 and 2076 bps in length with ORFs encoding peptides of 705 and 691 amino acids, respectively. To study the mechanism of the estradiol-17beta (E(2)) action, we examined the expression pattern of GTH subunit mRNAs in pituitary and GTH-receptor mRNAs in gonads, and the changes of plasma E(2) level when E(2) treatment was applied to immature black porgy. E(2) treatment increased mRNA expression levels of the genes and plasma E(2) levels, indicating that E(2) stimulated the increases in GTH subunit and GTH-receptor mRNAs. These data indicate that E(2) plays an important regulatory role in the brain-pituitary-gonad axis of immature black porgy. We provide the molecular characterization and expression of the GTH subunits and GTH receptors during sex change in the protandrous black porgy.
An
Kwang Wook
KW
Division of Marine Environment & BioScience, Korea Maritime University, Busan, Republic of Korea.
Lee
Ki-Young
KY
Yun
Sung Gyu
SG
Choi
Cheol Young
CY
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D037201:Follicle Stimulating Hormone, beta Subunit / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D015292:Glycoprotein Hormones, alpha Subunit / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006062:Gonadotropins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006066:Gonads / Q000187:drug effects / Q000378:metabolism; D057850:Hermaphroditic Organisms; D037101:Luteinizing Hormone, beta Subunit / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011967:Receptors, Gonadotropin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019849:Sex Determination Processes
D018076:DNA, Complementary; D037201:Follicle Stimulating Hormone, beta Subunit; D015292:Glycoprotein Hormones, alpha Subunit; D006062:Gonadotropins; D037101:Luteinizing Hormone, beta Subunit; D021122:Protein Subunits; D012333:RNA, Messenger; D011967:Receptors, Gonadotropin; D004958:Estradiol