Chemical structure of the somatic antigen isolated from Salmonella Abortusequi (O4).
A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide (LPS) of Salmonella Abortusequi O4 bacterium (previously serogroup B). As determined by compositional analyses and NMR spectroscopy, the O-polysaccharide consists of four or five residues in the repeating subunit. The assigned structures are: alpha-Abep -->2)-alpha-D-Manp-(1-->4)-alpha-L-Rhap-(1-->3)-alpha-D-Galp-(1--> and alpha-Abep alpha-D-Glcp -->2)-alpha-D-Manp-(1-->4)-alpha-L-Rhap-(1-->3)-alpha-D-Galp-(1-->. A distribution of the repeating units in O-chain was analysed by Western blotting with anti O12 serum and MS spectrometry of oligosaccharides obtained from partial hydrolysis of polysaccharide.
Kaczyński
Zbigniew
Z
Faculty of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952 Gdańsk, Poland. zbyszek@chem.univ.gda.pl
Gajdus
Jerzy
J
Dziadziuszko
Halina
H
Stepnowski
Piotr
P
D015153:Blotting, Western; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D019081:O Antigens / Q000737:chemistry*; D012475:Salmonella / Q000276:immunology*
D008070:Lipopolysaccharides; D019081:O Antigens