Hylin a1, the first cytolytic peptide isolated from the arboreal South American frog Hypsiboas albopunctatus ("spotted treefrog").
RP-HPLC fractionation of the electrically stimulated skin secretion of the arboreal South American frog Hypsiboas albopunctatus ("spotted treefrog") led to the isolation of a cytolytic C-terminally amidated peptide. This novel peptide, named hylin a1 (Hy-a1), consists of 18 amino acid residues (IFGAILPLALGALKNLIK-NH(2)). The alpha-helical structure of the synthetic hylin a1 peptide was confirmed by CD spectroscopy in the presence of 60% (v/v) TFE. The synthetic peptide displayed broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria including Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa and also against fungi (Candida albicans, C. krusei, C. parapsilosis and Cryptococcus neoformans). Hylin a1 was also able to disrupt human erytrocytes (HC(50)=18 microM). Similarity analysis using PSI-BLAST revealed 50-44% of identity to maximins Hv, H16, H15 and H10 from Bombina maxima and also to hylins b1 and b2 (Hy-b1 and Hy-b2) from Hypsiboas lundii (synonym: Hyla biobeba).
Castro
Mariana S
MS
Brazilian Center for Protein Research, Department of Cell Biology, University of Brasilia, CEP 70.910-900, Brasilia/DF, Brazil. mscastro@unb.br
Ferreira
Tânia Cristina G
TC
Cilli
Eduardo M
EM
Crusca
Edson
E
Mendes-Giannini
Maria José Soares
MJ
Sebben
Antonio
A
Ricart
Carlos André O
CA
Sousa
Marcelo V
MV
Fontes
Wagner
W
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification; D001001:Anura / Q000378:metabolism*; D002942:Circular Dichroism; D003600:Cytosol / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D010455:Peptides