Isothiocyanates repress estrogen receptor alpha expression in breast cancer cells.
The isothiocyanates (ITCs) have long been known to possess chemopreventive activities for a variety of neoplasms including breast cancer, but the molecular mechanism by which ITCs prevent breast cancer development has not been established. In this study, we investigated the effects of benzyl and phenethyl isothiocyanate (BITC and PEITC) on the estrogen-stimulated growth of estrogen receptor alpha (ERalpha)-positive breast cancer MCF7 and T-47D cells. BITC and PEITC inhibited estrogen-stimulated cell growth and reduced the expression levels of ERalpha in MCF7 and T-47D cells in a dose- and time-dependent and reversible manner. In addition, BITC and PEITC also abrogated the transcriptional activity of ERalpha and hence inhibited estrogen-stimulated expression of the estrogen responsive gene, pS2. These results demonstrated that BITC and PEITC function as potent ERalpha disruptors to abrogate mitogenic estrogen signaling in ER-positive breast cancer cells, which provides a molecular explanation for the growth inhibitory function of ITCs in breast cancer development, and a rational for further exploration of ITCs as chemopreventive agents for human mammary carcinogenesis.
Kang
Lianguo
L
Department of Surgery, Creighton University Medical School, Omaha, NE 68178, USA.
Ding
Ling
L
Wang
Zhao-Yi
ZY
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D000970:Antineoplastic Agents; D047628:Estrogen Receptor alpha; D017879:Isothiocyanates; C058305:phenethyl isothiocyanate; C031403:benzyl isothiocyanate