title: A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populations.
abstract: In a collaborative work carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG), a polymerase chain reaction multiplex was optimized in order to type ten X-chromosome short tandem repeats (STRs) in a single reaction, including: DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423. Using this X-decaplex, each 17 of the participating laboratories typed a population sample of approximately 200 unrelated individuals (100 males and 100 females). In this work, we report the allele frequencies for the ten X-STRs in 15 samples from Argentina (Buenos Aires, Córdoba, Río Negro, Entre Ríos, and Misiones), Brazil (São Paulo, Rio de Janeiro, Paraná, and Mato Grosso do Sul), Colombia (Antioquia), Costa Rica, Portugal (Northern and Central regions), and Spain (Galicia and Cantabria). Gene diversities were calculated for the ten markers in each population and all values were above 56%. The average diversity per locus varied between 66%, for DXS7133, and 82%, for DXS6809. For this set of STRs, a high discrimination power was obtained in all populations, both in males (> or =1 in 5 x 10(5)) and females (> or =1 in 3 x 10(9)), as well as high mean exclusion chance in father/daughter duos (> or =99.953%) and in father/mother/daughter trios (> or =99.999%). Genetic distance analysis showed no significant differences between northern and central Portugal or between the two Spanish samples from Galicia and Cantabria. Inside Brazil, significant differences were found between Rio de Janeiro and the other three populations, as well as between São Paulo and Paraná. For the five Argentinean samples, significant distances were only observed when comparing Misiones with Entre Ríos and with Río Negro, the only two samples that do not differ significantly from Costa Rica. Antioquia differed from all other samples, except the one from Río Negro.
authors.affiliation: IPATIMUP Institute of Pathology and Molecular Immunology, University of Porto, Porto, Portugal. lgusmao@ipatimup.pt
authors.forename: Leonor
authors.initials: L
authors.lastname: Gusmão
authors.forename: Paula
authors.initials: P
authors.lastname: Sánchez-Diz
authors.forename: Cíntia
authors.initials: C
authors.lastname: Alves
authors.forename: Iva
authors.initials: I
authors.lastname: Gomes
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Zarrabeitia
authors.forename: Mariel
authors.initials: M
authors.lastname: Abovich
authors.forename: Ivannia
authors.initials: I
authors.lastname: Atmetlla
authors.forename: Cecilia
authors.initials: C
authors.lastname: Bobillo
authors.forename: Luisa
authors.initials: L
authors.lastname: Bravo
authors.forename: Juan
authors.initials: J
authors.lastname: Builes
authors.forename: Laura
authors.initials: L
authors.lastname: Cainé
authors.forename: Raquel
authors.initials: R
authors.lastname: Calvo
authors.forename: Elizeu
authors.initials: E
authors.lastname: Carvalho
authors.forename: Mónica
authors.initials: M
authors.lastname: Carvalho
authors.forename: Regina
authors.initials: R
authors.lastname: Cicarelli
authors.forename: Laura
authors.initials: L
authors.lastname: Catelli
authors.forename: Daniel
authors.initials: D
authors.lastname: Corach
authors.forename: Marta
authors.initials: M
authors.lastname: Espinoza
authors.forename: Oscar
authors.initials: O
authors.lastname: García
authors.forename: Marcelo
authors.initials: M
authors.lastname: Malaghini
authors.forename: Joyce
authors.initials: J
authors.lastname: Martins
authors.forename: Fátima
authors.initials: F
authors.lastname: Pinheiro
authors.forename: Maria
authors.initials: M
authors.lastname: João Porto
authors.forename: Eduardo
authors.initials: E
authors.lastname: Raimondi
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Riancho
authors.forename: Amelia
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Anayanci
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Belén
authors.initials: B
authors.lastname: Rodríguez Cardozo
authors.forename: Vicente
authors.initials: V
authors.lastname: Schneider
authors.forename: Sandra
authors.initials: S
authors.lastname: Silva
authors.forename: Celso
authors.initials: C
authors.lastname: Tavares
authors.forename: Ulises
authors.initials: U
authors.lastname: Toscanini
authors.forename: Carlos
authors.initials: C
authors.lastname: Vullo
authors.forename: Martin
authors.initials: M
authors.lastname: Whittle
authors.forename: Iñaki
authors.initials: I
authors.lastname: Yurrebaso
authors.forename: Angel
authors.initials: A
authors.lastname: Carracedo
authors.forename: António
authors.initials: A
authors.lastname: Amorim
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D041321:Chromosomes, Human, X / Q000235:genetics*; D003364:Costa Rica; D016172:DNA Fingerprinting; D004252:DNA Mutational Analysis; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D040961:Genetic Drift; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005828:Genetics, Population; D006801:Humans; D007391:International Cooperation; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011174:Portugal; D011786:Quality Control; D013020:South America; D013030:Spain
chemical_list: D005819:Genetic Markers