title: Manufacturing of human placenta-derived mesenchymal stem cells for clinical trials.
abstract: Mesenchymal stem cells (MSC) are being used increasingly in clinical trials for a range of regenerative and inflammatory diseases. Bone marrow is the traditional source but is relatively inaccessible in large volume. MSC have now been derived from tissues other than bone marrow including placenta and adipose tissue. We have used placenta obtained after delivery as a source of MSC and have been unable to detect any marked differences from marrow-derived MSC in terms of cell surface phenotype, chemokine receptor display, mesodermal differentiation capacity or immunosuppressive ability. This report described our manufacturing process for isolating and expanding placenta-derived human MSC and their safe infusion into the first patient in a clinical trial program of human placenta-derived MSC.
authors.affiliation: Adult Stem Cell Laboratory, Biotherapy Program, Mater Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Gary
authors.initials: G
authors.lastname: Brooke
authors.forename: Tony
authors.initials: T
authors.lastname: Rossetti
authors.forename: Rebecca
authors.initials: R
authors.lastname: Pelekanos
authors.forename: Nina
authors.initials: N
authors.lastname: Ilic
authors.forename: Patricia
authors.initials: P
authors.lastname: Murray
authors.forename: Sonia
authors.initials: S
authors.lastname: Hancock
authors.forename: Vicki
authors.initials: V
authors.lastname: Antonenas
authors.forename: Gillian
authors.initials: G
authors.lastname: Huang
authors.forename: David
authors.initials: D
authors.lastname: Gottlieb
authors.forename: Ken
authors.initials: K
authors.lastname: Bradstock
authors.forename: Kerry
authors.initials: K
authors.lastname: Atkinson
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D017809:Fatal Outcome; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000628:therapy; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D010920:Placenta / Q000166:cytology*; D016896:Treatment Outcome; D055815:Young Adult