Using reverse genetics to develop small knockout collections for specific biological questions.
With the advent of indexed mutagenized insertion lines in Arabidopsis, it is now possible to order small knockout collections of particular genes to probe a question of biological interest. This first requires querying Arabidopsis databases to identify lines of interest, ordering them and then verifying homozygous lines to make your collection. Once the collection is in hand, it can be used multiple times to test scientific hypotheses as they arise.
Northey
Julian
J
Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
McCourt
Peter
P
D017360:Arabidopsis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D017353:Gene Deletion; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D017343:Genes, Plant; D018745:Genome, Plant; D044404:Genomic Islands; D016254:Mutagenesis, Insertional; D010935:Plant Diseases / Q000235:genetics; D030821:Plants, Genetically Modified
D004269:DNA, Bacterial; C036483:T-DNA