title: Characterization of different diamond-like carbon electrodes for biosensor design.
abstract: Diamond-like carbon (DLC) films are gaining big interest in electrochemistry research area. DLC electrodes made with different ratio of sp(3)/sp(2) carbon hybridization or doped with different percentages of nickel were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for the elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutaraldehyde. Measurements were made at a fixed potential+1.0V in 40mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose, 0.6875muA/mM, than SiCNi5%, 0.3654muA/mM. Detections limits were 20muM and 30muM, respectively. Apparent Michaelis-Menten constants were found around 3mM. Forty-eight percent and 79% of the original response for 0.5mM glucose remained after 10 days for both biosensors, respectively.
authors.affiliation: Center of Electrical Engineering of Lyon, CEGELY, UMR/CNRS 5005, Ecole Centrale Lyon, 69134 Ecully Cedex, France; Laboratory of Chemistry, Lebanese University, Fanar, Beirut, Lebanon; Laboratory of Analytical Electrochemistry, Claude Bernard University, Lyon I, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Maalouf
authors.forename: H
authors.initials: H
authors.lastname: Chebib
authors.forename: Y
authors.initials: Y
authors.lastname: Saikali
authors.forename: O
authors.initials: O
authors.lastname: Vittori
authors.forename: M
authors.initials: M
authors.lastname: Sigaud
authors.forename: F
authors.initials: F
authors.lastname: Garrelie
authors.forename: C
authors.initials: C
authors.lastname: Donnet
authors.forename: N
authors.initials: N
authors.lastname: Jaffrezic-Renault