Approaches to investigate the role of signaling in ER-to-Golgi transport.
There have been many indications that kinases play a role in signaling the transport of proteins through the secretory pathway. Specifically, the serine/threonine kinase Akt affects the transport of cholesterol regulatory components from the endoplasmic reticulum (ER) to the Golgi. However, elucidating the target of Akt in this process has proven to be challenging. Here we describe an approach devised to investigate the Akt target(s) based on examining a potential candidate.
Sharpe
Laura J
LJ
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
Du
Ximing
X
Brown
Andrew J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D016466:CHO Cells; D017124:Conserved Sequence; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D019143:Evolution, Molecular; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008853:Microscopy; D008967:Molecular Biology / Q000379:methods*; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction
D011506:Proteins; D011993:Recombinant Fusion Proteins; D051057:Proto-Oncogene Proteins c-akt