Connectivity of the primate superior colliculus mapped by concurrent microstimulation and event-related FMRI.
BACKGROUND
Neuroanatomical studies investigating the connectivity of brain areas have heretofore employed procedures in which chemical or viral tracers are injected into an area of interest, and connected areas are subsequently identified using histological techniques. Such experiments require the sacrifice of the animals and do not allow for subsequent electrophysiological studies in the same subjects, rendering a direct investigation of the functional properties of anatomically identified areas impossible.


RESULTS
Here, we used a combination of microstimulation and fMRI in an anesthetized monkey preparation to study the connectivity of the superior colliculus (SC). Microstimulation of the SC resulted in changes in the blood oxygenation level-dependent (BOLD) signals in the SC and in several cortical and subcortical areas consistent with the known connectivity of the SC in primates.


CONCLUSIONS
These findings demonstrates that the concurrent use of microstimulation and fMRI can be used to identify brain networks for further electrophysiological or fMRI investigation.
Field
Courtney B
CB
Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada.
Johnston
Kevin
K
Gati
Joseph S
JS
Menon
Ravi S
RS
Everling
Stefan
S
D000818:Animals; D016477:Artifacts; D001931:Brain Mapping / Q000379:methods*; D004558:Electric Stimulation; D008279:Magnetic Resonance Imaging; D008839:Microelectrodes; D010100:Oxygen / Q000097:blood; D011323:Primates / Q000502:physiology*; D013477:Superior Colliculi / Q000502:physiology*; D013997:Time Factors
D010100:Oxygen