title: Structure of the motor subunit of type I restriction-modification complex EcoR124I.
abstract: Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors.
authors.affiliation: Department of Structure and Function of Proteins, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Nove Hrady, Czech Republic.
authors.forename: Mikalai
authors.initials: M
authors.lastname: Lapkouski
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: Pavel
authors.initials: P
authors.lastname: Janscak
authors.forename: Ivana Kuta
authors.initials: IK
authors.lastname: Smatanova
authors.forename: Jannette
authors.initials: J
authors.lastname: Carey
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Ettrich
authors.forename: Eva
authors.initials: E
authors.lastname: Csefalvay
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D000255:Adenosine Triphosphate; C048242:endodeoxyribonuclease EcoR124I; D015253:Deoxyribonucleases, Type I Site-Specific