title: Dutch and Arctic mutant peptides of beta amyloid(1-40) differentially affect the FGF-2 pathway in brain endothelium.
abstract: Single point mutations of the amyloid precursor protein generate Abeta variants bearing amino acid substitutions at positions 21-23. These mutants are associated with distinct hereditary phenotypes of cerebral amyloid angiopathy, manifesting varying degrees of tropism for brain vessels, and impaired microvessel remodeling and angiogenesis. We examined the differential effects of E22Q (Dutch), and E22G (Arctic) variants in comparison to WT Abeta on brain endothelial cell proliferation, angiogenic phenotype expression triggered by fibroblast growth factor (FGF-2), pseudo-capillary sprouting, and induction of apoptosis. E22Q exhibited a potent anti-angiogenic profile in contrast to E22G, which had a much weaker effect. Investigations on the FGF-2 signaling pathway revealed the greatest differences among the peptides: E22Q and WT peptides suppressed FGF-2 expression while E22G had barely any effect. Phosphorylation of the FGF-2 receptor, FGFR-1, and the survival signal Akt were abolished by E22Q and WT peptides, but not by E22G. The biological dissimilar effect of the mutant and WT peptides on cerebral EC cannot be assigned to a particular Abeta structure, suggesting that the toxic effect of the Abeta assemblies goes beyond mere multimerization.
authors.affiliation: Department of Molecular Biology, University of Siena, Via A. Moro 2, 53100, Siena, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Solito
authors.forename: Federico
authors.initials: F
authors.lastname: Corti
authors.forename: Silvia
authors.initials: S
authors.lastname: Fossati
authors.forename: Emiliya
authors.initials: E
authors.lastname: Mezhericher
authors.forename: Sandra
authors.initials: S
authors.lastname: Donnini
authors.forename: Jorge
authors.initials: J
authors.lastname: Ghiso
authors.forename: Antonio
authors.initials: A
authors.lastname: Giachetti
authors.forename: Agueda
authors.initials: A
authors.lastname: Rostagno
authors.forename: Marina
authors.initials: M
authors.lastname: Ziche
mesh_terms: D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D001921:Brain / Q000098:blood supply*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D055806:Microvessels / Q000166:cytology; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D016222:Fibroblast Growth Factor 2; C496348:FGFR1 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1