From MEFs to matrigel I: passaging hESCs in the presence of MEFs.
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.
Zhang
Jin
J
David Geffen School of Medicine, University of California, Los Angeles, USA.
Khvorostov
Ivan
I
Teitell
Michael
M
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003094:Collagen; D004338:Drug Combinations; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007797:Laminin; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology*; D011509:Proteoglycans
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen