title: [Identification of a new carbohydrate-binding site of influenza virus].
abstract: It has recently been shown that the influenza virus can specifically bind the residue of a nonsialylated sulfated oligosaccharide Gal(6SO(3)H)beta1-4GlcNAcbeta (6'SLacNAc). To identify by photoaffinity labeling the virion component that binds 6'SLacNAc, we synthesized a carbohydrate probe containing a (125)I labeled diazocyclopentadien-2-yl carbonyl group as an aglycone. According to the electrophoretic data, the labeled areas corresponded to a large hemagglutinin subunit, a nucleocapsid protein, and neuraminidase (NA). Probing in the presence of an excess of 6'SLacNAcbeta-OCH(2)CH(2)NHAc glycoside resulted in redistribution of the labeling intensity, with the maximum inhibition being observed for NA. The data obtained indicate that NA is a viral 6'SLacNAc-binding protein.
authors.forename: E V
authors.initials: EV
authors.lastname: Parfinovich
authors.forename: L V
authors.initials: LV
authors.lastname: Mochalova
authors.forename: Iu G
authors.initials: IuG
authors.lastname: Molotkovskiĭ
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
authors.forename: E L
authors.initials: EL
authors.lastname: Vodovozova
mesh_terms: D001665:Binding Sites; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006389:Hemagglutinins, Viral / Q000737:chemistry; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry*; D009439:Neuraminidase / Q000737:chemistry; D019590:Nucleocapsid Proteins / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry*; D011485:Protein Binding; D014764:Viral Proteins / Q000737:chemistry*; D014771:Virion / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D006389:Hemagglutinins, Viral; D019590:Nucleocapsid Proteins; D009844:Oligosaccharides; D014764:Viral Proteins; D009439:Neuraminidase