title: Polymerase chain reaction typing of genes of the locus of enterocyte effacement of ruminant attaching and effacing Escherichia coil.
abstract: The variability of the tir, espA, and espD genes of the locus of enterocyte effacement (LEE) in 185 attaching and effacing Escherichia coli (AEEC) strains isolated from healthy and diarrheic cattle, sheep, and goats was investigated by polymerase chain reaction. Nineteen of the strains were enterohemorrhagic E. coli (EHEC); the other 166 were enteropathogenic E. coli (EPEC). The combinations of the tir and esp genes were associated with the variants of the eae gene but not with a strain's belonging to the EPEC or EHEC group, animal species, or health status (healthy or diarrheic) of the animal. In addition, most of the strains showed the same combinations of LEE genes and serogroups as have been found in AEEC strains isolated from humans, which indicates that ruminants seem to be an EPEC reservoir for humans.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Yuste
authors.forename: José A
authors.initials: JA
authors.lastname: Orden
authors.forename: Ricardo
authors.initials: R
authors.lastname: De La Fuente
authors.forename: José A
authors.initials: JA
authors.lastname: Ruiz-Santa-Quiteria
authors.forename: Dolores
authors.initials: D
authors.lastname: Cid
authors.forename: Susana
authors.initials: S
authors.lastname: Martínez-Pulgarín
authors.forename: Gustavo
authors.initials: G
authors.lastname: Domínguez-Bernal
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004197:Disease Reservoirs / Q000662:veterinary; D020895:Enterocytes / Q000382:microbiology; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D054308:Enteropathogenic Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D015511:Goat Diseases / Q000382:microbiology; D006041:Goats; D006304:Health Status; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011956:Receptors, Cell Surface; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C102051:EspA protein, E coli; C474481:EspD protein, E coli; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli