title: Heterologous expression and initial characterization of recombinant RbcX protein from Thermosynechococcus elongatus BP-1 and the role of RbcX in RuBisCO assembly.
abstract: In the cyanobacterial RuBisCO operon from Thermosynechococcus elongatus the rbcX gene is juxtaposed and cotranscribed with the rbcL and rbcS genes which encode large and small RuBisCO subunits, respectively. It has been suggested that the rbcX position is not random and that the RbcX protein could be a chaperone for RuBisCO. In this study, the RbcX protein from T. elongatus was overexpressed, purified and preliminary functional studies were conducted. The recombinant protein purified from Escherichia coli extracts was predominantly present in a soluble fraction in a dimeric form. Coexpression experiments have demonstrated that RbcX can mediate RbcL dimer (L(2)) formation, and that it is essential for the L(8) core complex assembly. This is the first characterization of the RbcX protein from a thermophilic organism.
authors.affiliation: Department of Biophysics, Faculty of Biotechnology, University of Wroclaw, Wrocław, Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Tarnawski
authors.forename: Beata
authors.initials: B
authors.lastname: Gubernator
authors.forename: Piotr
authors.initials: P
authors.lastname: Kolesinski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szczepaniak
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D015153:Blotting, Western; D002850:Chromatography, Gel; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D018832:Molecular Chaperones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D046940:Synechococcus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D018832:Molecular Chaperones; C522129:RbcX protein, cyanobacteria; D011994:Recombinant Proteins; D012273:Ribulose-Bisphosphate Carboxylase