The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants.
In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
Dzikiewicz-Krawczyk
Agnieszka
A
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
Piontek
Paulina
P
Szweykowska-Kulińska
Zofia
Z
Jarmołowski
Artur
A
D017398:Alternative Splicing; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D039502:Nuclear Cap-Binding Protein Complex / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D039502:Nuclear Cap-Binding Protein Complex; D012333:RNA, Messenger; D018749:RNA, Plant