title: Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells.
abstract: This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As(5+), Cr(6+), Cd(2+), and Pb(2+)) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr(6+) exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr(6+) lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 microg-Cr/mL-medium (p<0.05), respectively, but showed no significant difference at higher Cr(6+)concentrations (2.0-5.0 microg-Cr/mL-medium).
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: I-Cheng
authors.initials: IC
authors.lastname: Chou
authors.forename: Yee-Lin
authors.initials: YL
authors.lastname: Wu
authors.forename: Lin-Chi
authors.initials: LC
authors.lastname: Wang
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Chang-Chien
authors.forename: Hsinyu
authors.initials: H
authors.lastname: Lee
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity; D002104:Cadmium / Q000633:toxicity; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002857:Chromium / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D007854:Lead / Q000633:toxicity; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008670:Metals / Q000633:toxicity*; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D014162:Transfection
chemical_list: D008670:Metals; D000072317:Polychlorinated Dibenzodioxins; D002104:Cadmium; D002857:Chromium; D007854:Lead; D008156:Luciferases; D001151:Arsenic