title: Heart-cutting two-dimensional (size exclusion x reversed phase) liquid chromatography-mass spectrometry analysis of flavonol glycosides from leaves of Maytenus ilicifolia.
abstract: Two-dimensional liquid chromatography-electrospray ionization mass spectrometry was employed to analyze flavonol glycosides present in leaves of Maytenus ilicifolia, frequently used in traditional Brazilian medicine. Since they contain many flavonol glycosides, including isomers, one-dimensional liquid chromatography did not give complete separation and identification, yielding overlapping of compounds with different molecular weights. Thus, employing size exclusion chromatography in the first and reversed phase in the second dimension, a great number of flavonol glycosides could be identified and its relative abundances determined. The majority of glycosides contained kaempferol or quercetin as aglycones, and glycosides with previously unreported structures were also present and characterized.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba-PR, Brazil.
authors.forename: Lauro M
authors.initials: LM
authors.lastname: de Souza
authors.forename: Thales R
authors.initials: TR
authors.lastname: Cipriani
authors.forename: Carolina F
authors.initials: CF
authors.lastname: Sant'ana
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.forename: Philip A J
authors.initials: PA
authors.lastname: Gorin
authors.forename: Guilherme L
authors.initials: GL
authors.lastname: Sassaki
mesh_terms: D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D044948:Flavonols / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D044949:Kaempferols; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D029755:Maytenus / Q000737:chemistry*; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin
chemical_list: D044948:Flavonols; D006027:Glycosides; D044949:Kaempferols; C006552:kaempferol; D011794:Quercetin