title: AAV2/1-TNFR:Fc gene delivery prevents periodontal disease progression.
abstract: Periodontal disease is a chronic inflammatory condition induced by tooth-associated microbial biofilms that induce a host immune response. Therapeutic control of progressive tissue destruction in high-risk patients is a significant challenge in therapy. Soluble protein delivery of antagonists to tumor necrosis factor-alpha (TNF-alpha) inhibits alveolar bone resorption due to periodontitis. However, protein therapy raises several concerns, such as recurrence of disease activity after treatment cessation and repeated dosing regimens. In this study, we used pseudotyped adeno-associated virus vector based on serotype 1 (AAV2/1) to deliver the TNF receptor-immunoglobulin Fc (TNFR:Fc) fusion gene to rats subjected to experimental Porphyromonas gingivalis (Pg)-lipopolysaccharide (LPS)-mediated bone loss. Animals received Pg-LPS delivered to the gingivae thrice weekly for 8 weeks, vehicle alone, Pg-LPS and intramuscular delivery of pseudotyped AAV2/1-TNFR:Fc vector (1 x 10(11) DNase I-resistant particles) or AAV2/1-TNFR:Fc vector delivered to naive animals. AAV2/1-TNFR:Fc therapy led to sustained therapeutic levels of serum TNFR protein and protected against Pg-LPS-mediated loss of bone volume and density. Furthermore, AAV2/1-TNFR:Fc administration reduced local levels of multiple proinflammatory cytokines and osteoclast-like cells at the periodontal lesions. These findings suggest that delivery of AAV2/1-TNFR:Fc may be a viable approach to modulate periodontal disease progression.
authors.affiliation: Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Cirelli
authors.forename: C H
authors.initials: CH
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: MacKool
authors.forename: M
authors.initials: M
authors.lastname: Taba
authors.forename: K H
authors.initials: KH
authors.lastname: Lustig
authors.forename: H
authors.initials: H
authors.lastname: Burstein
authors.forename: W V
authors.initials: WV
authors.lastname: Giannobile
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology / Q000517:prevention & control; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood; D015519:Bone Density; D002454:Cell Differentiation; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D000229:Dependovirus / Q000235:genetics; D018450:Disease Progression; D000068800:Etanercept; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000097:blood / Q000235:genetics*; D007166:Immunosuppressive Agents / Q000097:blood; D008297:Male; D010010:Osteoclasts / Q000473:pathology; D010518:Periodontitis / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood / Q000235:genetics*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D018124:Receptors, Tumor Necrosis Factor; D000068800:Etanercept