Binding assays for abscisic acid receptors.
The plant hormone abscisic acid (ABA) is a major growth regulator mediating several aspects of plant development and stress-induced processes. For the hormone to function within the cell, it must first interact with a proteinaceous receptor to commence transmission of a signal to downstream components that elicit a cellular response. With the identification of several ABA receptors, there is an increased interest in receptor-ABA interaction studies and a demand for easy-to-follow protocols to allow measurement and quantification of ABA binding. Here, we present protocols for radioligand-binding measurements with particular focus on the ABA-binding of the recently identified ABA receptors, ABAP1 and FCA.
Razem
Fawzi A
FA
Plant Science Department, University of Manitoba, Winnipeg, MB, Canada.
Hill
Robert D
RD
D000040:Abscisic Acid / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods*
D010940:Plant Proteins; D000040:Abscisic Acid