title: Sperm and embryo analysis in a carrier of supernumerary inv dup(15) marker chromosome.
abstract: We identified a small, paternally inherited, supernumerary marker chromosome, inv dup(15), in a phenotypically normal and normozoospermic male from a couple with reproductive problems. Sperm analysis by fluorescence in situ hybridization (FISH) showed that the marker was present in 26% of sperm nuclei. The disomy 15 was 10 times higher than in normal control donors. FISH analysis for aneuploidies of the other chromosomes showed an increase in nondisjunction of chromosome 21. We also examined 24 embryos by preimplantation genetic diagnosis, and 10 embryos (41.7%) contained the marker. This report provides information about inheritance of inv dup(15) from a male carrier.
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Oracova
authors.forename: Petra
authors.initials: P
authors.lastname: Musilova
authors.forename: Olga
authors.initials: O
authors.lastname: Kopecna
authors.forename: Roman
authors.initials: R
authors.lastname: Rybar
authors.forename: Miluse
authors.initials: M
authors.lastname: Vozdova
authors.forename: Katerina
authors.initials: K
authors.lastname: Vesela
authors.forename: Jiri
authors.initials: J
authors.lastname: Rubes
mesh_terms: D000328:Adult; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D004622:Embryo, Mammalian; D005260:Female; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D010375:Pedigree; D011247:Pregnancy; D013094:Spermatozoa; D024182:Uniparental Disomy / Q000235:genetics*