title: 2006 GEP-ISFG collaborative exercise on mtDNA: reflections about interpretation, artefacts, and DNA mixtures.
abstract: We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.
authors.affiliation: Comisaría General de Policía Científica, DNA Laboratory, Madrid, Spain. lourditasmt@ya.com
authors.forename: L
authors.initials: L
authors.lastname: Prieto
authors.forename: A
authors.initials: A
authors.lastname: Alonso
authors.forename: C
authors.initials: C
authors.lastname: Alves
authors.forename: M
authors.initials: M
authors.lastname: Crespillo
authors.forename: M
authors.initials: M
authors.lastname: Montesino
authors.forename: A
authors.initials: A
authors.lastname: Picornell
authors.forename: A
authors.initials: A
authors.lastname: Brehm
authors.forename: J L
authors.initials: JL
authors.lastname: Ramírez
authors.forename: M R
authors.initials: MR
authors.lastname: Whittle
authors.forename: M J
authors.initials: MJ
authors.lastname: Anjos
authors.forename: I
authors.initials: I
authors.lastname: Boschi
authors.forename: J
authors.initials: J
authors.lastname: Buj
authors.forename: M
authors.initials: M
authors.lastname: Cerezo
authors.forename: S
authors.initials: S
authors.lastname: Cardoso
authors.forename: R
authors.initials: R
authors.lastname: Cicarelli
authors.forename: D
authors.initials: D
authors.lastname: Comas
authors.forename: D
authors.initials: D
authors.lastname: Corach
authors.forename: C
authors.initials: C
authors.lastname: Doutremepuich
authors.forename: R M
authors.initials: RM
authors.lastname: Espinheira
authors.forename: I
authors.initials: I
authors.lastname: Fernández-Fernández
authors.forename: S
authors.initials: S
authors.lastname: Filippini
authors.forename: Julia
authors.initials: J
authors.lastname: Garcia-Hirschfeld
authors.forename: A
authors.initials: A
authors.lastname: González
authors.forename: B
authors.initials: B
authors.lastname: Heinrichs
authors.forename: A
authors.initials: A
authors.lastname: Hernández
authors.forename: F P N
authors.initials: FP
authors.lastname: Leite
authors.forename: R P
authors.initials: RP
authors.lastname: Lizarazo
authors.forename: A M
authors.initials: AM
authors.lastname: López-Parra
authors.forename: M
authors.initials: M
authors.lastname: López-Soto
authors.forename: J A
authors.initials: JA
authors.lastname: Lorente
authors.forename: B
authors.initials: B
authors.lastname: Mechoso
authors.forename: I
authors.initials: I
authors.lastname: Navarro
authors.forename: S
authors.initials: S
authors.lastname: Pagano
authors.forename: J J
authors.initials: JJ
authors.lastname: Pestano
authors.forename: J
authors.initials: J
authors.lastname: Puente
authors.forename: E
authors.initials: E
authors.lastname: Raimondi
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Quesada
authors.forename: M F
authors.initials: MF
authors.lastname: Terra-Pinheiro
authors.forename: L
authors.initials: L
authors.lastname: Vidal-Rioja
authors.forename: C
authors.initials: C
authors.lastname: Vullo
authors.forename: A
authors.initials: A
authors.lastname: Salas
mesh_terms: D016477:Artifacts; D001801:Blood Stains; D019411:Clinical Laboratory Techniques / Q000592:standards*; D003198:Computer Simulation; D004247:DNA / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000592:standards*; D004272:DNA, Mitochondrial / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D003627:Data Interpretation, Statistical; D016208:Databases, Factual; D005260:Female; D005554:Forensic Medicine; D005819:Genetic Markers; D006197:Hair / Q000737:chemistry; D006239:Haplotypes; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011786:Quality Control; D012015:Reference Standards; D012463:Saliva / Q000737:chemistry
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D004247:DNA