Specific binding sites for progesterone and 17beta-estradiol in cells of Triticum aestivum L.
The presence and location of specific binding sites for progesterone and 17beta-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [(3)H]progesterone and [(3)H]17beta-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [(3)H]17beta-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17beta-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.
Janeczko
Anna
A
Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland. ania@belanna.strefa.pl
Budziszewska
Bogusława
B
Skoczowski
Andrzej
A
Dybała
Małgorzata
M
D000818:Animals; D001665:Binding Sites; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D008024:Ligands; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D014908:Triticum / Q000166:cytology / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D008024:Ligands; D011374:Progesterone; D004958:Estradiol