title: [Promoting effect of constituents in plasma after oral administration of liuwei dihuangwan on proliferation of rat osteoblast].
abstract: OBJECTIVE
  To elucidate the promoting effect of the constituents in plasma after oral administration of Liuwei Dihuangwan on proliferation of rat osteoblast.
  
  
  METHODS
  To add the constituents in plasma after oral administration of Liuwei Dihuangwan with rational concentration into culture solution of rat osteoblast, later using MT method for determining the proliferation rate of rat osteoblast.
  
  
  RESULTS
  The Mixed group including morroniside, sweroside and loganin with different dose all significantly promoted proliferation of rat osteoblast.
  
  
  CONCLUSIONS
  This study preliminarily elucidated the constituents such as morroniside, sweroside and loganin in plasma after oral administration of Liuwei Dihuangwan were main biological constituents which contributed to anti-osteoporosis bioactivity of Liuwei Dihuangwan.
authors.affiliation: College of Pharmacy, Heilongliang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Li
authors.forename: Xi-jun
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005960:Glucosides / Q000097:blood; D006027:Glycosides / Q000097:blood; D057888:Iridoid Glucosides; D039823:Iridoids / Q000097:blood; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010949:Plasma / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D057888:Iridoid Glucosides; D039823:Iridoids; C488401:morroniside; C059516:loganin; C049412:sweroside