Remnant lipoprotein cholesterol homogenous assay (RemL-C) is closely associated with very-low-density lipoprotein remnants: comparison with the immunoseparation assay.
BACKGROUND
Remnant-like particle-cholesterol (RLP-C) is recognized as a risk factor for cardiovascular disease. As an alternative to the immunoseparation assay widely used for the measurement of RLP-C, a new remnant lipoprotein-C homogenous assay (RemL-C) is available. In light of its homogeneity as an assay method, we speculated that this homogeneous assay (RemL-C) is closely associated with very-low-density lipoprotein(VLDL) remnant including intermediate-density lipoprotein(IDL). We examined the characteristics of the homogeneous assay for reacting with VLDL remnants.


RESULTS
VLDL1, VLDL2, and IDL were separated by ultracentrifugation in the fasting serum of subjects including hypertriglyceridemia and uremic patients usually having higher levels of remnants. While RemL-C and RLP-C were mainly recovered in VLDL1 and both assays were strongly correlated with serum TG and VLDL1, the RemL-C assay was more closely correlated with VLDL2 and IDL levels than the RLP-C assay. RemL-C levels were significantly correlated with IDL-C, whereas RLP-C levels had only borderline associations with IDL-C (r= 0.56 Vs. 0.31).


CONCLUSIONS
The remnant lipoprotein cholesterol homogenous assay is more closely associated with VLDL2 and IDL than the immunoseparation assay.
Nagashima
Masaharu
M
Department of Diabetes, Metabolism and Endocrinology, Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8666, Japan.
Mori
Yusaku
Y
Morita
Ryo
R
Takada
Michiya
M
Lee
Soushou
S
Taira
Takayasu
T
Miyauchi
Kazuhito
K
Kuwata
Hideyuki
H
Kayahara
Norihiko
N
Hirano
Tsutomu
T
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000209:etiology; D002784:Cholesterol / Q000097:blood*; D015243:Cholesterol, VLDL / Q000097:blood*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D007118:Immunoassay / Q000379:methods*; D008074:Lipoproteins / Q000097:blood*; D008875:Middle Aged; D006435:Renal Dialysis; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood*; D014511:Uremia / Q000097:blood
D015415:Biomarkers; D015243:Cholesterol, VLDL; D008074:Lipoproteins; D014280:Triglycerides; C112017:remnant-like particle cholesterol; D002784:Cholesterol