Antiperoxidation activity of triterpenoids from rhizome of Anemone raddeana.
Four triterpenoid compounds hederacolchiside E (1), hederasaponin B (2), raddeanoside 20 (3) and raddeanoside 21 (4) were isolated from ethanol extracts of rhizome of Anemone raddeana Regel. The effects of these triterpenoids on superoxide generation, tyrosyl phosphorylation of proteins and translocation of cytosolic compounds, such as p47(phox), p67(phox) and Rac to the cell membrane in human neutrophils was investigated. The superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was slightly suppressed by hederasaponin B, raddeanoside 20 and raddeanoside 21 in a concentration dependent manner. The superoxide generation induced by arachidonic acid (AA) was suppressed by hederasaponin B and raddeanoside 21 significantly. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins: p47(phox), p67(phox), and Rac to the cell membrane were suppressed in parallel with the suppression of stimulus-induced superoxide generation.
Chen
Xin
X
Department of Medicinal Plants, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Lu
Jincai
J
He
Wenfei
W
Chi
Haidong
H
Yamashita
Koichi
K
Manabe
Masanobu
M
Kodama
Hiroyuki
H
D031883:Anemone / Q000737:chemistry; D002462:Cell Membrane / Q000502:physiology; D003600:Cytosol / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D015394:Molecular Structure; D019255:NADPH Oxidases / Q000502:physiology; D009504:Neutrophils / Q000187:drug effects; D010750:Phosphoproteins / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects*; D027343:Rhizome; D013481:Superoxides / Q000037:antagonists & inhibitors*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014443:Tyrosine / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000502:physiology
D016166:Free Radical Scavengers; D010750:Phosphoproteins; D010936:Plant Extracts; D014315:Triterpenes; C079789:neutrophil cytosol factor 67K; D013481:Superoxides; D014443:Tyrosine; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D020744:rac GTP-Binding Proteins