title: [Extraction of triterpenoids from fruiting [corrected] bodies of Ganoderma lucidum by supercritical fluid extraction].
abstract: OBJECTIVE
  To optimize the extracting condition for triterpenoids from the fruits of Ganoderma lucidum by supercritical fluid extraction (SFE).
  
  
  METHODS
  Optimum extraction conditions were studied by orthogonal tests. The total triterpenoids were determined by ultraviolet spectrophotometry and ganoderic acid B was determined by RP-HPLC.
  
  
  RESULTS
  The optimal extraction conditions were that the pressure 25 MPa, the temperature was 45 degrees C, the extraction time was 1.5 hour, and the ethanol was employed as modifier carrier at the volume of 3 mL x g(-1).
  
  
  CONCLUSIONS
  SFE is superior and it is feasible to extract triterpenoids from fruits of Ganoderma lucidum.
authors.affiliation: Jiangsu Provincial Academy of Chinese Medicine, Jiangsu Engineering Research Center for Pharmaceutical Preparation, Nanjing 210028, China.
authors.forename: Shi-hua
authors.initials: SH
authors.lastname: Song
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Jia
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Wang
authors.forename: Cheng-lei
authors.initials: CL
authors.lastname: Zhao
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D020940:Reishi / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D014315:Triterpenes