PP13 mRNA expression in trophoblasts from preeclamptic placentas.
To assess the role of placental protein 13 (PP13) in the pathogenesis of preeclampsia, the messenger RNA expression of PP13 was investigated in the trophoblasts from first and third trimester placenta. First, syncytiotrophoblasts and extravillous trophoblasts were separated from frozen section of third trimester placenta obtained from pregnant women with and without preeclampsia by laser microdissection. The PP13 levels of syncytiotrophoblasts were significantly higher than those of extravillous trophoblasts. The expression levels in syncytiotrophoblasts from the preeclampsia were significantly lower than those from normal. Next, the PP13 expression was assessed in trophoblasts from residual samples of chorionic villus sampling at 11 weeks of gestation, who subsequently developed preeclampsia. The levels in the preeclampsia group were significantly lower than those of normal cases. These findings indicate that an alteration in the PP13-messenger RNA expression in the trophoblasts may be associated with the pathogenesis of preeclampsia.
Sekizawa
Akihiko
A
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan. sekizawa@med.showa-u.ac.jp
Purwosunu
Yuditiya
Y
Yoshimura
Shiho
S
Nakamura
Masamitsu
M
Shimizu
Hanako
H
Okai
Takashi
T
Rizzo
Nicola
N
Farina
Antonio
A
D000328:Adult; D015193:Chorionic Villi Sampling; D005260:Female; D037161:Galectins / Q000235:genetics*; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000737:chemistry*
D037161:Galectins; C038195:LGALS13 protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases