Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis.
OBJECTIVE
The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats.


METHODS
Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software.


RESULTS
Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group.


CONCLUSIONS
The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.
Potu
Bhagath Kumar
BK
Department of Anatomy, Kasturba Medical College, Manipal University.
Rao
Muddanna S
MS
Kutty
N Gopalan
NG
Bhat
Kumar M R
KM
Chamallamudi
Mallikarjuna Rao
MR
Nayak
Soubhagya Ranjan
SR
D000473:Alkanes; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D032422:Cissus / Q000737:chemistry*; D005260:Female; D047109:Fetal Development / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D023382:Toxicity Tests, Acute
D000473:Alkanes; D010936:Plant Extracts; C004544:naphtha