title: Age-related enlargement of lymphoid tissue and altered leukocyte composition in serglycin-deficient mice.
abstract: Serglycin (SG) is a proteoglycan that is located predominantly in the secretory granules of hematopoietic cells. Previous studies have established a crucial role for SG in promoting the storage of various secretory granule compounds that are of importance in the immune defense system. Here, we show that mice lacking SG spontaneously develop enlargement of multiple lymphoid organs, including the spleen, Peyer's patches (PP), and bronchus-associated lymphoid tissue. In the spleen, the lack of SG resulted in a significant decrease in the proportion of CD4(+) cells as well as an increase of the CD45RC(+) leukocyte population, indicating an expansion of naïve lymphocytes. In the PP, the lack of SG resulted in a general increase in cellularity, without significant alterations in the proportion of individual leukocyte populations. The enlargement of lymphoid tissues was not accompanied by increased serum levels of inflammatory cytokines. The number of mast cells in the peritoneum was not affected by the lack of SG, as judged by surface staining for CD117 (c-kit). However, the intensity of c-kit staining was reduced significantly in SG null animals. Moreover, the number of peritoneal macrophages, defined by morphological criteria and by CD11b staining, was decreased markedly in older, SG-deficient animals. Finally, experiments in which airway inflammation was induced by bacterial LPS revealed a more pronounced inflammatory response in old, SG-deficient as compared with wild-type mice. Taken together, our data show that SG deficiency causes multiple, age-related effects on the lymphoid system.
authors.affiliation: Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry, Uppsala, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Wernersson
authors.forename: Tiago
authors.initials: T
authors.lastname: Braga
authors.forename: Osama
authors.initials: O
authors.lastname: Sawesi
authors.forename: Ida
authors.initials: I
authors.lastname: Waern
authors.forename: Kajsa E
authors.initials: KE
authors.lastname: Nilsson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Pejler
authors.forename: Magnus
authors.initials: M
authors.lastname: Abrink
mesh_terms: D000367:Age Factors; D000818:Animals; D001980:Bronchi; D002452:Cell Count; D007249:Inflammation / Q000276:immunology; D008221:Lymphoid Tissue / Q000473:pathology*; D017737:Macrophages, Peritoneal / Q000166:cytology; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D010581:Peyer's Patches; D011509:Proteoglycans / Q000172:deficiency*; D013154:Spleen; D033921:Vesicular Transport Proteins / Q000172:deficiency*
chemical_list: D011509:Proteoglycans; D033921:Vesicular Transport Proteins; C075430:serglycin