title: An avian cell line designed for production of highly attenuated viruses.
abstract: Several viral vaccines, including highly promising vectors such as modified vaccinia Ankara (MVA), are produced on chicken embryo fibroblasts. Dependence on primary cells complicates production especially in large vaccination programs. With primary cells it is also not possible to create packaging lines for replication-deficient vectors that are adapted to proliferation in an avian host. To obviate requirement for primary cells permanent lines from specific tissues of muscovy duck were derived (AGE1.CR, CS, and CA) and further modified: we demonstrate that stable expression of the structural gene pIX from human adenovirus increases titers for unrelated poxvirus in the avian cells. This augmentation appears to be mediated via induction of heat shock and thus provides a novel cellular substrate that may allow further attenuation of vaccine strains.
authors.affiliation: ProBioGen AG, Goethestr. 54, 13086 Berlin, Germany. ingo.jordan@probiogen.de
authors.forename: Ingo
authors.initials: I
authors.lastname: Jordan
authors.forename: Ad
authors.initials: A
authors.lastname: Vos
authors.forename: Stefanie
authors.initials: S
authors.lastname: Beilfuss
authors.forename: Andreas
authors.initials: A
authors.lastname: Neubert
authors.forename: Sabine
authors.initials: S
authors.lastname: Breul
authors.forename: Volker
authors.initials: V
authors.lastname: Sandig
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D004372:Ducks; D006801:Humans; D014616:Vaccinia virus / Q000254:growth & development*; D010948:Viral Plaque Assay; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus