title: Determination of in vivo disulfide-bonded proteins in Arabidopsis.
abstract: Protein thiol-disulfide oxidoreduction plays an important role in redox regulation of cellular processes. Here we present a proteomic approach to visualize and map in vivo disulfide-bonded proteins in plants. A proteomic map of the disulfide-bonded proteins was achieved using 2D gel electrophoresis of Arabidopsis protein extract. Along with novel proteins identified as potentially redox regulated, we have also shown the feasibility of mapping some of the cysteines involved in the formation of disulfide bonds. This study presents an important tool for characterizing redox-regulated proteins.
authors.affiliation: Department of Botany and Zoology, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Sophie
authors.initials: S
authors.lastname: Alvarez
authors.forename: Gordon H
authors.initials: GH
authors.lastname: Wilson
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid; D004220:Disulfides / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D053719:Tandem Mass Spectrometry
chemical_list: D029681:Arabidopsis Proteins; D004220:Disulfides