In silico identification of genes in bacteriophage DNA.
One of the most satisfying aspects of a genome sequencing project is the identification of the genes contained within it.These are of two types: those which encode tRNAs and those which produce proteins. After a general introduction on the properties of protein-encoding genes and the utility of the Basic Local Alignment Search Tool (BLASTX) to identify genes through homologs, a variety of tools are discussed by their creators. These include for genome annotation: GeneMark, Artemis, and BASys; and, for genome comparisons: Artemis Comparison Tool (ACT), Mauve, CoreGenes, and GeneOrder.
Kropinski
Andrew M
AM
Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
Borodovsky
Mark
M
Carver
Tim J
TJ
Cerdeño-Tárraga
Ana M
AM
Darling
Aaron
A
Lomsadze
Alexandre
A
Mahadevan
Padmanabhan
P
Stothard
Paul
P
Seto
Donald
D
Van Domselaar
Gary
G
Wishart
David S
DS
D001435:Bacteriophages / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D012984:Software
D004279:DNA, Viral