Total internal reflected resonance light scattering determination of protein in human blood serum at water/tetrachloromethane interface with Arsenazo-TB and Cetyltrimethylammonium bromide.
A direct quantification of protein in human blood serum samples is proposed based on the measurement of total internal reflected resonance light scattering (TIR-RLS) at water/tetrachloromethane (H(2)O/CCl(4)) interfaces. At pH 4.10 and in the presence of Cetyltrimethylammonium bromide (CTAB), the interaction of Arsenazo-TB (ATB) with human serum albumin (HSA) results in strong enhanced TIR-RLS signals with the maximum spectra peak located at 330nm. The enhanced TIR-RLS intensity at 330nm was found to be proportional to the concentration of HSA in the range 0.5-42.3microg/ml and its limit of determination (3sigma) is 198ng/ml. The content of HSA in blood serum samples were determined with the recovery of 97-103% for standard addition experiment.
Dong
Lijun
L
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Chen
Xingguo
X
Hu
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