[Chromatographic determination of caffeine and its metabolites in saliva and plasma].
The authors made a comparative analysis of the well-known high performance liquid chromatographic (HPLC) techniques to detect caffeine and its primary metabolites in saliva and plasma when during their oral administration. The studies yielded a new specific and sensitive procedure for HPLC analysis of caffeine and its major metabolites, whose distinctive feature was to simultaneously separate six substances with a possibility of determining the activity of the cytochrome P-450 isozymes 1A2, 2E1, and 3A4.
Filimonova
A A
AA
Ziganshina
L E
LE
Chichirov
A A
AA
D002110:Caffeine / Q000032:analysis / Q000097:blood / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D006801:Humans; D012015:Reference Standards; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002110:Caffeine; D019392:Cytochrome P-450 CYP2E1; C504573:CYP1A2 protein, human; D019388:Cytochrome P-450 CYP1A2; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human