Effect of polyamines and silver nitrate on the high frequency regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria; sp. asiatica).
In this study, we have investigated the effect ofpolyamines (PA) and silver nitrate (AgNO3) on the high frequency regeneration from cotyledon explants of bottle gourd containing Murashige and Skoog (MS) media supplemented with different kind of Cytokinin alone or in the combination. Synergistic effect of kinetin (1 mg L(-1)) and benzyl adenine (BA) (2 mg L(-1)) itself showed highest shoot regeneration (80.6%) efficiency than BA or Kinetin alone in cotyledon explants of bottle gourd without adding AgNO3 or PAs. We have also observed that PAs and AgNO3 show their sensitivity on the regeneration, which is hormonal dependent. Regenerated shoots were rooted in half strength MS media containing 0.1 mg L(-1) IAA.
Shyamali
Saha
S
Laboratory of Plant Genetics and Breeding, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Hattori
Kazumi
K
D002455:Cell Division / Q000187:drug effects; D018548:Cotyledon / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D019662:Cucurbitaceae / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D007701:Kinetin / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D011073:Polyamines / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D012835:Silver Nitrate / Q000494:pharmacology*
D011073:Polyamines; D012835:Silver Nitrate; D007701:Kinetin