HSP70 and mucin 5B: novel protein targets of N,N'-dinitrosopiperazine-induced nasopharyngeal tumorigenesis.
N,N'-Dinitrosopiperazine (DNP) induces nasopharyngeal carcinoma (NPC) and shows organ specificity to the nasopharyngeal epithelium. To investigate its mechanism, the rat NPC model was induced using DNP. Rat NPC and normal nasopharyngeal cells were obtained from the NPC model using laser capture. The total proteins from these cell samples were separated with two-dimension polyacrylamide gel electrophoresis techniques, and highly expressed proteins (> five-fold) were analyzed using matrix-assisted laser desorption/ionization time of flight and bioinformatics. The results showed that HSP70 and mucin 5B expression increased not only in rat NPC but also in atypical hyperplasia nasopharyngeal tissues, a precancer stage of NPC. High-expression of heat shock protein 70 (HSP70) and mucin 5B was further supported by western blot analysis. The immunofluorescence and western-blotting studies further showed that DNP induced the expression of HSP70 and mucin 5B in a dosage-dependent manner in normal nasopharyngeal epithelia cells. Our data indicate that DNP triggers over-expression of HSP70 and mucin 5B, and is involved in nasopharyngeal tumorigenesis. HSP70 and mucin 5B may be important targets in nasopharyngeal tumorigenesis induced by DNP.
Tang
Fa-Qing
FQ
Xiangya Hospital, Department of Pathology, Xiangya School of Medicine, Central South University,Changsha, Hunan, China. tangfaqing333@hotmail.com
Duan
Chao-Jun
CJ
Huang
Da-Mao
DM
Wang
Wei-Wei
WW
Xie
Chun-Lei
CL
Meng
Jing-Jing
JJ
Wang
Lei
L
Jiang
Hai-Ying
HY
Feng
De-Yun
DY
Wu
Shang-Hui
SH
Gu
Huan-Hua
HH
Li
Mo-Yu
MY
Deng
Fu-Liang
FL
Gong
Zhi-Jun
ZJ
Zhou
Hui
H
Xu
Yong-Hao
YH
Tan
Can
C
Zhang
Xin
X
Cao
Ya
Y
D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens / Q000633:toxicity*; D049109:Cell Proliferation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D055257:Mucin-5B / Q000378:metabolism*; D009303:Nasopharyngeal Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009602:Nitrosamines / Q000633:toxicity; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002273:Carcinogens; D018840:HSP70 Heat-Shock Proteins; D055257:Mucin-5B; D009602:Nitrosamines; C026156:N,N'-dinitrosopiperazine