Suppression of allergic airway inflammation and IgE responses by a class I restricted allergen peptide vaccine.
CD8 T cells are known to deviate CD4 T-cell responses from Th2 toward Th1. Reduction of Th2 cytokines and increased interferon-gamma ameliorates allergic airway disease. We have developed a novel approach to the suppression of allergic airway inflammation, by designing a MHC class I-restricted allergen peptide vaccine, which induces potent and long-lived CD8 T-cell responses. Vaccination of C57BL/6 mice before allergen sensitization completely prevented allergen-specific immunoglobulin E (IgE) antibody responses. Vaccination after sensitization failed to suppress IgE, but inhibited accumulation of eosinophils and neutrophils in airways after subsequent allergen challenge. Vaccination suppressed Th2 airway infiltration and enhanced the lung Th1 response without inducing excessive CD8 cellular infiltration or interleukin-17, and the combination of class I peptide with adjuvant was more effective than adjuvant alone. Airway hyperreactivity was prevented by vaccination in an allergen-specific fashion. Class I peptide vaccines might therefore represent a robust and long-lasting immunotherapeutic strategy in allergic disease.
Wells
J W
JW
King's College London, MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Guy's Hospital, London, UK.
Choy
K
K
Lloyd
C M
CM
Noble
A
A
D000485:Allergens / Q000276:immunology*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003888:Desensitization, Immunologic; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology*
D000485:Allergens; D015395:Histocompatibility Antigens Class I; D022223:Vaccines, Subunit; D007073:Immunoglobulin E