title: Characterization of a C3 and a factor B-like in the carpet-shell clam, Ruditapes decussatus.
abstract: The alternative pathway is considered to be the most ancient route for activation of the complement system. Herein, we report the characterization of C3 and factor B-like proteins in the clam Ruditapes decussatus, termed Rd-C3 and Rd-Bf-like. The Rd-C3 is a three-chain protein, similar to other protoC3 proteins, and the Rd-Bf-like is composed of two complement control protein modules (CCP domains) that differ from other described Bf proteins. The inoculation of clams with live bacteria did not result in induction of these functions, but inhibited the expression of Rd-C3 and Rd-Bf-like.
authors.affiliation: Instituto de Investigaciones Marinas, CSIC, C/Eduardo Cabello 6, 36208 Vigo, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Prado-Alvarez
authors.forename: J
authors.initials: J
authors.lastname: Rotllant
authors.forename: C
authors.initials: C
authors.lastname: Gestal
authors.forename: B
authors.initials: B
authors.lastname: Novoa
authors.forename: A
authors.initials: A
authors.lastname: Figueras
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D003176:Complement C3 / Q000737:chemistry / Q000235:genetics*; D011415:Complement Factor B / Q000737:chemistry / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000502:physiology
chemical_list: D003176:Complement C3; D011415:Complement Factor B