Combining proteomics and metabolite analyses to unravel cadmium stress-response in poplar leaves.
A proteomic analysis of poplar leaves exposed to cadmium, combined with biochemical analysis of pigments and carbohydrates revealed changes in primary carbon metabolism. Proteomic results suggested that photosynthesis was slightly affected. Together with a growth inhibition, photoassimilates were less needed for developmental processes and could be stored in the form of hexoses or complex sugars, acting also as osmoprotectants. Simultaneously, mitochondrial respiration was upregulated, providing energy needs of cadmium-exposed plants.
Kieffer
Pol
P
Centre de Recherche Public-Gabriel Lippmann, L-4422 Belvaux, Luxembourg, Belgium.
Planchon
Sébastien
S
Oufir
Mouhssin
M
Ziebel
Johanna
J
Dommes
Jacques
J
Hoffmann
Lucien
L
Hausman
Jean-François
JF
Renaut
Jenny
J
D000595:Amino Acid Sequence; D002104:Cadmium / Q000633:toxicity*; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects; D011108:Polymers / Q000737:chemistry; D032107:Populus / Q000187:drug effects* / Q000254:growth & development*; D040901:Proteomics / Q000379:methods*; D015854:Up-Regulation
D002241:Carbohydrates; D011108:Polymers; C024617:polyol; D002104:Cadmium; D002734:Chlorophyll; D002244:Carbon