title: The pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts.
abstract: To investigate the pro-angiogenic factors stimulated by human papillomavirus type 16 E6 and E7 protein in C33A and human fibroblasts. HPV-16 E6 and E7 genes were transfected into C33A and HFB to detect the profiling of angiogenesis-associated factors with the TranSignal angiogenesis antibody array. The mRNA and protein levels of the cytokines were examined by traditional RT-PCR and Western blotting in both cell lines before and after transfection of HPV-16 E6 and E7. HPV-16 E6 and E7 genes were successfully transfected into C33A and HFB cells. On the sheet of antibody array, after transfection of HPV-16 E6 and E7, 6 other cytokines, Ang-1, FGFalpha, HGF, IL-6, IP-10 and PIGF besides VEGF, were detected at higher levels in C33A cells. Expression of 7 other cytokines besides IL-8, Ang-1, IL-1alpha, IL-1beta, HGF, IL-6, VEGF and PIGF increased in HFB cells. The common cytokines in both cell lines were Ang-1, HGF, PIGF and VEGF. The mRNA and protein levels of the four cytokines were verified to increase by traditional RT-PCR and Western blotting in both cell lines after transfection of HPV-16 E6 and E7. Multiple pro-angiogenic cytokines could be stimulated by HPV-16 E6 and E7 protein both in cervical cancer cell line and normal human diploid cells. Anti-angiogenesis therapy may be effective alone or in combination with biologic means aimed at E6 and E7 in the treatment of cervical cancer.
authors.affiliation: Cancer Biology Research Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430030, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xi
authors.forename: Shixuan
authors.initials: S
authors.lastname: Wang
authors.forename: Changyu
authors.initials: C
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.forename: Xun
authors.initials: X
authors.lastname: Tian
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Dongrui
authors.initials: D
authors.lastname: Deng
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D040081:Protein Array Analysis; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000821:virology*
chemical_list: D043925:Angiogenesis Inducing Agents; D016207:Cytokines; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16