title: [Effect of calming the liver and suppressing the hyperactive YANG drugs on lymphocyte protein in hypertension patients with hyperactivity of liver-YANG].
abstract: OBJECTIVE
  To explore the effect of calming the liver and suppressing the hyperactive YANG drugs on the lymphocyte protein and clinical efficacy in the hypertension patients with hyperactivity of liver-YANG, and to identify the therapy.
  
  
  METHODS
  Twenty-six hypertension patients with hyperactivity of liver-YANG were treated by calming the liver and suppressing the hyperactive YANG drugs for 2 courses. Symptoms of Chinese medicine and blood pressure were observed, and the separated lymphocyte total protein of normal and hypertensions before and after the treatment were examined by the solid-state pH gradient 2-dimensional gel electrophoresis. The differences of the protein expression were analyzed by ImageMaster 2DE analysis software with two-way patterns.
  
  
  RESULTS
  The total efficiency rate of calming the liver and suppressing the hyperactive YANG drugs was 88.5%, and the drugs could significantly relieve the symptoms, such as headache, dizziness, dry mouth, irritability, etc. Calming the liver and suppressing the hyperactive YANG drugs could also remarkably reduce the blood pressure,with significant different between pre-treatment and post-treatment (P<0.05). The average spots of lymphocyte gel proteins in the normal and the hypertension patients with syndrome of hyperactivity of liver-YANG before and after the treatment were 527+/-41,559+/-62, and 543+/-59, respectively. Compared with normal people, the expression of 15 proteins was down-regulated, and 10 up-regulated in the hypertension patients with syndrome of hyperactivity of liver-YANG. Compared with the pre-treatment, the expression of 12 proteins was increased in the 15 down-regulated proteins, and 6 decreased in the 10 up-regulated proteins after the treatment in the hypertension patients with syndrome of the hyperactivity of liver-YANG.
  
  
  CONCLUSIONS
  Calming the liver and suppressing the hyperactive yang drugs may mildly depress the blood pressure and improve the symptoms of Chinese medicine. The effect of drugs in treating hypertension may probably be associated with regulating the expression of some proteins in lymphocytes.
authors.affiliation: Research Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. zgw7512@sina.com
authors.forename: Guang-wei
authors.initials: GW
authors.lastname: Zhong
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Luo
authors.forename: Guo-lin
authors.initials: GL
authors.lastname: Chen
authors.forename: Zhen-jia
authors.initials: ZJ
authors.lastname: Yi
authors.forename: Ze-qi
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Chuan-man
authors.initials: CM
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000188:drug therapy*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D008517:Phytotherapy; D011506:Proteins / Q000378:metabolism*; D016709:Yin-Yang
chemical_list: D004365:Drugs, Chinese Herbal; D011506:Proteins