The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice.
Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.
Anisimov
Andrey P
AP
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Bakhteeva
Irina V
IV
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Panfertsev
Evgeniy A
EA
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Svetoch
Tat'yana E
TE
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Kravchenko
Tat'yana B
TB
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Platonov
Mikhail E
ME
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Titareva
Galina M
GM
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Kombarova
Tat'yana I
TI
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Ivanov
Sergey A
SA
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
Rakin
Alexander V
AV
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkofer Str. 9a, 80336 Munich, Germany.
Amoako
Kingsley K
KK
Canadian Food Inspection Agency Lethbridge Laboratory, PO 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada.
Dentovskaya
Svetlana V
SV
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia.
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D005260:Female; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D014774:Virulence; D015009:Yersinia Infections / Q000276:immunology* / Q000382:microbiology; D015010:Yersinia pestis / Q000235:genetics* / Q000472:pathogenicity*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C081814:pH 6 antigen, Yersinia