title: Evaluation of an injectable, photopolymerizable, and three-dimensional scaffold based on methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) combined with autologous mesenchymal stem cells in a goat tibial unicortical defect model.
abstract: An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and alpha-tricalcium phosphate (alpha-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of alpha-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.
authors.affiliation: Department of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. geert.vertenten@ugent.be
authors.forename: Geert
authors.initials: G
authors.lastname: Vertenten
authors.forename: Evi
authors.initials: E
authors.lastname: Lippens
authors.forename: Jordi
authors.initials: J
authors.lastname: Gironès
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gorski
authors.forename: Heidi
authors.initials: H
authors.lastname: Declercq
authors.forename: Jimmy
authors.initials: J
authors.lastname: Saunders
authors.forename: Wim
authors.initials: W
authors.lastname: Van den Broeck
authors.forename: Koen
authors.initials: K
authors.lastname: Chiers
authors.forename: Luc
authors.initials: L
authors.lastname: Duchateau
authors.forename: Etiene
authors.initials: E
authors.lastname: Schacht
authors.forename: Maria
authors.initials: M
authors.lastname: Cornelissen
authors.forename: Frank
authors.initials: F
authors.lastname: Gasthuys
authors.forename: Lieven
authors.initials: L
authors.lastname: Vlaminck
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D002469:Cell Separation; D002478:Cells, Cultured; D006041:Goats; D007150:Immunohistochemistry; D007267:Injections; D008027:Light; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D008954:Models, Biological; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology* / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011091:Polyesters; C062968:lactide-caprolactone copolymer