Enhanced cell proliferation and neuroblast differentiation in the rat hippocampal dentate gyrus following myocardial infarction.
Basic and clinical studies have revealed that depression is frequently observed following myocardial infarction (MI). We observed changes in neurons in the subgranular zone of the hippocampal dentate gyrus (DG) 14 days after chronic cardiac ischemia. Cresyl violet staining was conducted to examine neurodegeneration. Cresyl violet-positive neurons in the hippocampus in the MI-operated group were similar to those in the sham-operated group, and Fluoro-Jade B-positive cells were not observed in either group. Next, we observed changes in cell proliferation using Ki67 and in the differentiation of neuroblasts using doublecortin (DCX) in the DG. The number of Ki67- and DCX-positive cells in the subgranular zone of the DG in the MI-operated group was significantly increased compared to that in the sham-operated group. In addition, DCX-positive processes were prominent in the MI group. These results suggest that MI may influence cell proliferation and affect neuroblast differentiation in the subgranular zone of the DG.
Hwang
In Koo
IK
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Yoo
Ki-Yeon
KY
Han
Tae Hee
TH
Lee
Choong Hyun
CH
Choi
Jung Hoon
JH
Yi
Sun Shin
SS
Lee
So Yeong
SY
Ryu
Pan Dong
PD
Yoon
Yeo Sung
YS
Won
Moo-Ho
MH
D000818:Animals; D048588:Benzoxazines; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000502:physiology*; D003866:Depressive Disorder / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005452:Fluoresceins; D020925:Hypoxia-Ischemia, Brain / Q000150:complications; D019394:Ki-67 Antigen / Q000032:analysis / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism; D009203:Myocardial Infarction / Q000150:complications*; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D009416:Nerve Regeneration / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D009479:Neuropeptides / Q000032:analysis / Q000378:metabolism; D009930:Organic Chemicals; D010078:Oxazines; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D048588:Benzoxazines; D015415:Biomarkers; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005452:Fluoresceins; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D009930:Organic Chemicals; D010078:Oxazines; C435731:fluoro jade; C028911:cresyl violet