Haplotype diversity in human mitochondrial DNA hypervariable regions I-III in the city of Caracas (Venezuela).
In order to expand the database of variable DNA for forensic identification purposes in Venezuela, we analyzed the sequence polymorphisms of mitochondrial DNA (mtDNA) hypervariable regions (HVR) I-III from 100 unrelated individuals from the city of Caracas, using PCR amplification and fluorescent-based capillary electrophoresis sequencing method. Dominant haplogroups corresponded to Native Americans followed by African ones. The inclusion of HVR III although useful for sub-haplogroup assignation, added little to the discrimination capacity of our database.
Lander
N
N
Centro de Biotecnología, Fundación Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela.
Rojas
M G
MG
Chiurillo
M A
MA
Ramírez
J L
JL
D044383:Black People / Q000235:genetics; D022801:Complementarity Determining Regions / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary; D053803:Forensic Genetics / Q000379:methods; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D016133:Polymerase Chain Reaction; D014505:Urban Population; D014687:Venezuela
D022801:Complementarity Determining Regions; D017931:DNA Primers; D004272:DNA, Mitochondrial