Phage proteomics: applications of mass spectrometry.
Current techniques in mass spectrometry (MS) allow sensitive and accurate identification of proteins thanks to the in silico availability of these protein sequences within databases. This chapter provides a short overview of MS techniques used in the identification of phage structural proteins and focuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. Such analyses provide an experimental examination of structural proteins and confirm genome-based gene predictions.
Lavigne
Rob
R
Department of Biosystems, Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
Ceyssens
Pieter-Jan
PJ
Robben
J
J
D001435:Bacteriophages / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism
D014764:Viral Proteins