Quantitative determination of chlorogenic acid in Honeysuckle using microwave-assisted extraction followed by nano-LC-ESI mass spectrometry.
The chlorogenic acid (CA) in Honeysuckle is determined and identified by nano-liquid chromatography-electrospray ionization mass spectrometry (nano-LC-ESI/MS) after extraction with microwave-assisted extraction (MAE). As a new sample preparation method for Honeysuckle, the MAE procedure is optimized, validated and compared with conventional methods including reflux extraction (RE) and ultrasonic extraction (USE). It is found that MAE gives the best result due to the highest extraction efficiency within shortest extraction time (only 4 min). Here, CA is determined by nano-LC-ESI/MS based on the calibration curve of its authentic standard. The method linearity, detection limit, precision and recovery are studied. The results show that the combined MAE and nano-LC-ESI/MS method has a linearity (R(2) 0.991, 0.8-20 ng mL(-1)), a low limit of detection (0.5 ng mL(-1)), good precision (R.S.D.=2.54%) and a recovery (84.8%). The experiment has demonstrated that the nano-LC-ESI/MS following MAE is a fast and reliable method for quantitative analysis of CA in Honeysuckle.
Hu
Fengli
F
Department of Chemistry, Fudan University, Shanghai 200433, China.
Deng
Chunhui
C
Liu
Yang
Y
Zhang
Xiangmin
X
D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002726:Chlorogenic Acid / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004867:Equipment Design; D031239:Lonicera / Q000378:metabolism*; D008872:Microwaves; D036103:Nanotechnology / Q000379:methods; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics
D002726:Chlorogenic Acid