title: Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV.
abstract: Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Here we addressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. A redox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.
authors.affiliation: Department of Neurology and Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
authors.forename: Uma D
authors.initials: UD
authors.lastname: Vempati
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
authors.forename: Carlos T
authors.initials: CT
authors.lastname: Moraes
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D018345:Mice, Knockout
chemical_list: D045304:Cytochromes c; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I