[Promoting effect of constituents in plasma after oral administration of liuwei dihuangwan on proliferation of rat osteoblast].
OBJECTIVE
To elucidate the promoting effect of the constituents in plasma after oral administration of Liuwei Dihuangwan on proliferation of rat osteoblast.


METHODS
To add the constituents in plasma after oral administration of Liuwei Dihuangwan with rational concentration into culture solution of rat osteoblast, later using MT method for determining the proliferation rate of rat osteoblast.


RESULTS
The Mixed group including morroniside, sweroside and loganin with different dose all significantly promoted proliferation of rat osteoblast.


CONCLUSIONS
This study preliminarily elucidated the constituents such as morroniside, sweroside and loganin in plasma after oral administration of Liuwei Dihuangwan were main biological constituents which contributed to anti-osteoporosis bioactivity of Liuwei Dihuangwan.
Sun
Hui
H
College of Pharmacy, Heilongliang University of Chinese Medicine, Harbin 150040, China.
Zhang
Ning
N
Li
Li-jing
LJ
Wang
Xi-jun
XJ
D000284:Administration, Oral; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005960:Glucosides / Q000097:blood; D006027:Glycosides / Q000097:blood; D057888:Iridoid Glucosides; D039823:Iridoids / Q000097:blood; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010949:Plasma / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D057888:Iridoid Glucosides; D039823:Iridoids; C488401:morroniside; C059516:loganin; C049412:sweroside