title: Potential use of DNA adducts to detect mutagenic compounds in soil.
abstract: In this study, three different soils with contrasting features, spiked with 300 mg benzo[a]pyrene (BaP)/kg dry soil, were incubated at 20 degrees C and 60% water holding capacity for 540 days. At different time points, BaP and DNA were extracted and quantified, and DNA adducts were quantified by (32)P-postlabelling. After 540 days incubation, 69.3, 81.6 and 83.2% of initial BaP added remained in Cruden Bay, Boyndie and Insch soils, respectively. Meanwhile, a significantly different amount of DNA-BaP adducts were found in the three soils exposed to BaP over time. The work demonstrates the concept that DNA adducts can be detected on DNA extracted from soil. Results suggest the technique is not able to directly reflect bioavailability of BaP transformation products. However, this new method provides a potential way to detect mutagenic compounds in contaminated soil and to assess the outcomes of soil remediation.
authors.affiliation: School of Biology, Institute for Research on the Environment and Sustainability, Devonshire Building, Newcastle University, NE1 7RU, UK.
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Hua
authors.forename: Brett
authors.initials: B
authors.lastname: Lyons
authors.forename: Ken
authors.initials: K
authors.lastname: Killham
authors.forename: Ian
authors.initials: I
authors.lastname: Singleton
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis*; D001682:Biological Availability; D018736:DNA Adducts / Q000032:analysis*; D054750:Ecotoxicology / Q000379:methods; D009153:Mutagens / Q000032:analysis*; D010761:Phosphorus Radioisotopes / Q000032:analysis; D012987:Soil / Q000032:analysis*; D012989:Soil Pollutants / Q000032:analysis*; D013997:Time Factors
chemical_list: D018736:DNA Adducts; D009153:Mutagens; D010761:Phosphorus Radioisotopes; D012987:Soil; D012989:Soil Pollutants; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene