Effect of growth temperature and pH on the aminopeptidase activity of Pseudomonas putida, P. fluorescens and Flavobacterium odoratum; the 4-nitroaniline test is reliable.
No significant difference (p > 0.05) was observed in the specific aminopeptidase activity (SAA) developed by Pseudomonas fluorescens, P. putida and Flavobacterium odoratum either growing at pH 5.0-6.5 or at 7 and 12 degrees C. Nevertheless, a significant difference was found when comparing the SAA of these organisms. The SAA of F. odoratum was lower than those of pseudomonads. The 4-nitroaniline test is reliable to estimate the G(-) load of fresh food products.
Lopez-Tomas
L A
LA
Servicio de Bromatología e Higiene de los Alimentos, Centro Militar de Veterinaria de la Defensa, Ministerio de Defensa, 28024, Madrid, Spain.
Ordoñez
J A
JA
Mediavilla
C
C
Rodriguez-Marin
J L
JL
Sarmiento
P
P
Zamora
A
A
Garcia de Fernando
G
G
D000626:Aminopeptidases / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media; D005417:Flavobacterium / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000254:growth & development; D016958:Pseudomonas putida / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature
D000814:Aniline Compounds; D001426:Bacterial Proteins; D003470:Culture Media; C019498:4-nitroaniline; D000626:Aminopeptidases