Determination of microcystin-LR in water from Lake Tai, China.
Microcystin-LR (MC-LR) is a heptapeptide hepatotoxin produced by cyanobacteria. Immunoaffinity chromatography (IAC) column was prepared with CNBr-activated Sepharose 4B and monoclonal antibody of MC-LR. Water sample was cleaned up by IAC column and the content of MC-LR in water was determined by liquid chromatography-mass spectrometry (LC-MS). The results suggested that the IAC column exhibited highly selective specificity for MC-LR and selective removed interference from complex water sample. Water sample was concentrated for 2,000-fold through once purification. Cyanobacterial blooms had broken out in 2007 in Lake Tai, the third largest freshwater lake in China. Water samples from two parts of Lake Tai had been analyzed. The highest concentration of MC-LR in water from Lake Tai was 0.522 microg/L.
Xiao
Fu-Gang
FG
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China. xfug@163.com
Zhao
Xiao-Lian
XL
Tang
Jian
J
Gu
Xiao-Hong
XH
Zhang
Jing-Ping
JP
Niu
Wei-Min
WM
D000911:Antibodies, Monoclonal / Q000276:immunology; D002681:China; D002853:Chromatography, Liquid; D000458:Cyanobacteria / Q000254:growth & development; D008387:Marine Toxins; D013058:Mass Spectrometry; D052998:Microcystins / Q000032:analysis*; D014871:Water Microbiology
D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR