title: Anti-inflammatory effect of buckwheat sprouts in lipopolysaccharide-activated human colon cancer cells and mice.
abstract: In conducting an in vitro screening of ethanol extracts from various natural foods using a human colon cancer cell line (CoLoTC cells), an extract of buckwheat sprouts (ExtBS) was found to express significant anti-inflammatory activity. The anti-inflammatory activity of ExtBS was confirmed by oral administration of lipopolysaccharide (LPS) to mice. Inflammatory cytokines (interleukin 6 and tumor necrosis factor alpha) were markedly up-regulated in the spleen and liver from LPS-administrated mice, and combinatory treatment with LPS and ExtBS decreased up-regulation of them in both cytokines. Both serum cytokine levels corresponded to their gene expressions in tissues, but no anti-inflammatry effect in mice was observed when ExtBS was treated intraperitoneally. ExtBS oral administration also showed protective activity as to hepatic injury induced by galactosamine/LPS treatment. Based on these data, we suggest that ExtBS contains anti-inflammatory compounds.
authors.affiliation: Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. ishiis@obihiro.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishii
authors.forename: Takafumi
authors.initials: T
authors.lastname: Katsumura
authors.forename: Chikara
authors.initials: C
authors.lastname: Shiozuka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ooyauchi
authors.forename: Kunito
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Takigawa
authors.forename: Tatsunobu
authors.initials: T
authors.lastname: Fukushima
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Tokuji
authors.forename: Mikio
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Masao
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Mika
authors.initials: M
authors.lastname: Kawahara
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohba
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D005688:Galactosamine / Q000633:toxicity; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000293:injuries; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018520:Plant Shoots / Q000737:chemistry*; D012015:Reference Standards; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010936:Plant Extracts; D005688:Galactosamine