Optimization of bacitracin production by Bacillus licheniformis B5.
Production of the polypeptide antibiotic bacitracin by a newly isolated Bacillus licheniformis B5 strain, using a laboratory medium was optimized. A medium of the following conditions was obtained: 1.5% glycerol as carbon source, 0.05% glutamic acid as nitrogen source, 1.43% inorganic phosphate, 0.0025% magnesium sulfate, with an initial pH of 7.5. Culture was incubated at 37 degrees C for 48 h. The bacitracin productivity was about 174.2 units mL(-1). Bacitracin antibiotic was also produced using immobilized cells of the B5 isolate in different immobilization supports (7.5% polyacrylamide, 4% sodium alginate beads and 2% agar). It was found that the highest bacitracin production rate (192 units mL(-1)) was obtained when using 7.5% polyacrylamide gel as an immobilization material.
Haddar
Houria Ouled
HO
Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq.
Aziz
Ghazi Munim
GM
Al-Gelawi
Majed Hussein
MH
D001407:Bacillus / Q000378:metabolism*; D001414:Bacitracin / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D018698:Glutamic Acid; D005990:Glycerol; D008278:Magnesium Sulfate; D010710:Phosphates
D010710:Phosphates; D001414:Bacitracin; D018698:Glutamic Acid; D008278:Magnesium Sulfate; D005990:Glycerol