[Effects of PCB153 on DNA damage and DNA repair-related genes expressions induced by PBDE-47 in human neuroblastoma cells in vitro].
OBJECTIVE
To explore the effects of PCB153 on DNA damage and DNA repair-related gene expressions induced by PBDE-47 in SH-SY5Y cells.


METHODS
SH-SY5Y cells were incubated with different concentrations of 1,5 and 10 micromol/L PBDE-47 or/and 5 micromol/L PCB153 and antioxidant n-acetyl cysteine (NAC 100 micromol/L) for 24h in vitro, percentage of DNA in the tail, olive tail moment, the mRNA expression levels of XRCC1 and XRCC3 were measured respectively.


RESULTS
PBDE-47 increased percentage of DNA in the tail and olive tail moment significantly at doses of 5 micromol/L and above in a concentration-dependent manner compared to the control (P < 0.05). The percentage of DNA in the tail and olive tail moment were significantly higher in cells treated by 5 micromol/L PBDE-47 + 5 micromol/L PCB153 and 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared to the corresponding doses of PBDE-47 groups or PCB153 group (P < 0.05). The percentage of DNA in the tail and olive tail moment of cells treated by the groups added NAC were obviously lower than that of their corresponding groups not added it (P < 0.05). The interactive action was observed between PBDE-47 and PCB153 at concentrations of 10 micromol/L PBDE-47 (F = 23.74, P < 0.01). Significant decreased XRCC1 mRNA expression were observed at 10 micromol/L PBDE-47, 5 micromol/L PBDE-47 + 5 micromol/L PCB153, and 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups while significant increased XRCC3 mRNA expression were observed at 10 micromol/L PBDE-47 and 10 micromol/L PBDE-47 + 5 micromol/L PCB153 compared to the control group (P < 0.05). XRCC1 mRNA expression in cells treated by 100 micromol/L NAC + 10 micromol/L PBDE-47 + 5 micromol/L PCB153 group was significantly higher than that in 10 micromol/L PBDE-47 + 5 micromol/L PCB153 group (P < 0.05). XRCC3 mRNA expression in cells treated by PBDE-47 antioxidant groups were significantly lower than that in their corresponding non-antioxidant groups (P < 0.05). Correlation analysis showed there was a negative relationship between DNA damage and XRCC1 mRNA expression while a positive relationship between DNA damage and XRCC3 mRNA expression (r1 = 0.74, r2 = 0.76, P < 0.05).


CONCLUSIONS
PBDE-47 can induce DNA damage, PBDE-47 combined with PCB153 may increase the effects on DNA damage in SH-SY5Y cells in vitro, oxidative stress may play a important role in DNA damage induced by PBDE-47.
Gao
Ping
P
Department of Occupational and Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
He
Weihong
W
He
Ping
P
Xu
Bayi
B
D045744:Cell Line, Tumor; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000633:toxicity*
D004268:DNA-Binding Proteins; D055768:Halogenated Diphenyl Ethers; C511295:2,2',4,4'-tetrabromodiphenyl ether; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl