title: Monosaccharide inhibition of adherence by Pseudomonas aeruginosa to canine corneocytes.
abstract: The effect of D-galactose, D-mannose, L-rhamnose and dextrose on the adhesion to canine corneocytes by three strains of Pseudomonas aeruginosa was studied in six healthy dogs. Canine corneocytes were collected from the inner aspect of the pinna using adhesive discs (D-Squame). Half millimetre of bacterial suspension in phosphate-buffered saline (PBS) with or without the addition of a monosaccharide was placed over the corneocyte layer and incubated in moist chambers. Image analysis was used to quantify bacterial adherence to corneocytes. The three strains of Pseudomonas adhered well to canine corneocytes. All monosaccharides tested inhibited the adherence of Pseudomonas to canine corneocytes. The mean reduction in adhesion for individual sugars at a concentration of 0.1% was 40.2% (dextrose), 30.8% (L-rhamnose), 25.6% (D-galactose) and 19.4% (D-mannose). When D-galactose, D-mannose and L-rhamnose were used in combination at 0.1% concentration, the mean reduction in adherence was 52.9%. The monosaccharides studied may have a potential role in the management of Pseudomonas infections in dogs.
authors.affiliation: Faculty of Veterinary Science, Small Animal Teaching Hospital, The University of Liverpool, Leahurst, Chester High Road, Neston, Wirral CH64 7TE, UK. n.a.mcewan@liverpool.ac.uk
authors.forename: Neil A
authors.initials: NA
authors.lastname: McEwan
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Rème
authors.forename: Hugo
authors.initials: H
authors.lastname: Gatto
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Nuttall
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology*; D004283:Dog Diseases; D004285:Dogs; D009005:Monosaccharides / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000502:physiology*
chemical_list: D009005:Monosaccharides