title: An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP.
abstract: Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, South Korea.
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Sang Kyu
authors.initials: SK
authors.lastname: Kim
authors.forename: Kyoungsook
authors.initials: K
authors.lastname: Park
authors.forename: Hong-Kun
authors.initials: HK
authors.lastname: Lyu
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Sang J
authors.initials: SJ
authors.lastname: Chung
authors.forename: Wan Soo
authors.initials: WS
authors.lastname: Yun
authors.forename: Moonil
authors.initials: M
authors.lastname: Kim
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000737:chemistry*; D008320:Maltose / Q000737:chemistry*; D058619:Maltose-Binding Proteins; D011487:Protein Conformation; D012666:Semiconductors
chemical_list: D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D008320:Maltose