title: Nanoscale separation of molecular species based on their rotational mobility.
abstract: We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.
authors.affiliation: Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Testa
authors.forename: Andreas
authors.initials: A
authors.lastname: Schönle
authors.forename: Claas
authors.initials: C
authors.lastname: von Middendorff
authors.forename: Claudia
authors.initials: C
authors.lastname: Geisler
authors.forename: Rebecca
authors.initials: R
authors.lastname: Medda
authors.forename: Christian A
authors.initials: CA
authors.lastname: Wurm
authors.forename: Andre C
authors.initials: AC
authors.lastname: Stiel
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Mariano
authors.initials: M
authors.lastname: Bossi
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
authors.forename: Alexander
authors.initials: A
authors.lastname: Egner
mesh_terms: D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D012399:Rotation
chemical_list: D001704:Biopolymers