title: The leucine-responsive regulatory protein, Lrp, modulates microcin J25 intrinsic resistance in Escherichia coli by regulating expression of the YojI microcin exporter.
abstract: Many Escherichia coli K-12 strains display an intrinsic resistance to the peptide antibiotic microcin J25. In this study, we present results showing that the leucine-responsive regulatory protein, Lrp, is involved in this phenotype by acting as a positive regulator of YojI, a chromosomally encoded efflux pump which expels microcin out of cells. Exogenous leucine antagonizes the effect of Lrp, leading to a diminished expression of the pump and an increased susceptibility to microcin J25.
authors.affiliation: Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas--Universidad Nacional de Tucumán), Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Sergio B
authors.initials: SB
authors.lastname: Socías
authors.forename: Paula A
authors.initials: PA
authors.lastname: Vincent
authors.forename: Raúl A
authors.initials: RA
authors.lastname: Salomón
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007930:Leucine / Q000494:pharmacology; D051756:Leucine-Responsive Regulatory Protein / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D018528:ATP-Binding Cassette Transporters; D001430:Bacteriocins; D029968:Escherichia coli Proteins; C538747:YojI protein, E coli; D051756:Leucine-Responsive Regulatory Protein; C020411:microcin; D007930:Leucine