[Antileukemic mechanism of resveratrol in vitro and in mice bearing L1210-tumor].
OBJECTIVE
To elucidate the molecular mechanism of resveratrol against leukemia both in vitro and in vivo.


METHODS
Three kinds of leukemia cell lines, HuT-78, Jurkat and L1210 cells, were used in this study. After different doses of Res treatment, methyl thiazolyl tetrazolium (MTT) colorimetry was used to detect the cell proliferation; apoptosis was detected by flow cytometry; Western blot and immuno-precipitation method were used to detect the Bcl-2 and Bax proteins expression and the activity of phospho-signal transducer and activator of transcription 3 (p-STAT3). Besides, a total of 40 BALB/c mice were randomly divided into untreated group and 12.5, 25 and 50 mg/(kg.d) Res groups. Then, leukemia-bearing model was established by L1210 cells subcutaneous injection. Interleukin-6 (IL-6) was detected to determine the secretion function of T lymphocytes of the mice; the IL-6 mRNA expression in the liver tissue of mice was also detected by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of the p-STAT3 protein was measured by Western blot and immunohistochemical method.


RESULTS
The results indicated that resveratrol could inhibit the proliferation of HuT-78, Jurkat and L1210 cells and significantly induce the cell apoptosis. At the same time, the radio of Bcl-2/Bax and expression of p-STAT3 protein were decreased either. Furthermore, resveratrol could reduce the expression of IL-6 mRNA and intracellular content of IL-6, and decrease the expression of p-STAT3 protein in the liver of leukemic mice at a dose-dependent manner.


CONCLUSIONS
Resveratrol can function as an antileukemic agent through inducing apoptosis, modulating IL-6 and STAT3 both in vitro and in vivo.
Li
Tan
T
Department of Immunology, Medical College of the Chinese People's Armed Police Force, Tianjin 300162, China. tanli20042001@yahoo.com.cn
Wang
Wei
W
Li
Tong
T
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D000077185:Resveratrol; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000970:Antineoplastic Agents; D015850:Interleukin-6; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D013267:Stilbenes; D051028:bcl-2-Associated X Protein; D000077185:Resveratrol