Enantioselective separation of chiral vicinal diols in capillary electrophoresis using a mono-6(A)-aminoethylamino-beta-cyclodextrin as a chiral selector.
This paper describes an improved access to mono-6(A)-aminoethylamino-beta-CD (beta-CDen), a very efficient cationic chiral selector for CZE in the separation of eight chiral aromatic vicinal diols. The beta-CDen concentration has a strong influence on the efficiency of enantioseparation. The effects of the pH and concentration of the BGE, the capillary temperature, and the applied voltage on the resolution and separation selectivity have been studied. Excellent chiral resolution was achieved under the optimal conditions of beta-CDen 10 mM, pH 10, 200 mM borate buffer at 15 kV and 20 degrees C within 20 min. Moreover, the developed method was successfully applied to the determination of the enantiomeric purity of the catalytic asymmetric dihydroxylation (AD) reaction products.
Liu
Peng
P
Research Center for Chirotechnology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Sun
Xiaoli
X
He
Wei
W
Jiang
Ru
R
Wang
Pingan
P
Zhao
Yan
Y
Zhang
Shengyong
S
D000438:Alcohols / Q000302:isolation & purification*; D001881:Borates / Q000737:chemistry; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013696:Temperature; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry
D000438:Alcohols; D001881:Borates; D047392:beta-Cyclodextrins