Postnatal nutrition alters body composition in adult offspring exposed to maternal protein restriction.
The insulin-like growth factor (IGF) system is altered with intra-uterine growth retardation and in adult metabolic disease. The aim of the present study was to observe effects of continued protein restriction on the IGF-I system and body composition in offspring of mothers fed a low-protein (LP) diet. Offspring from Wistar dams fed either a 20 % (CON) or 8 % (LP) protein diet during gestation and lactation were studied at birth, 10 d, weaning and at 12 weeks after maintenance on either the 8 % (lp) or 20 % (con) protein diet from weaning. LP offspring had reduced weaning weights (P < 0.05) and reduced serum insulin (P < 0.005). Serum IGF-I (P < 0.001) and acid-labile subunit (ALS) (P < 0.0001) were reduced at 10 and 21 d. Hepatic expression of IGF-I (P < 0.05) and ALS (P < 0.005) were reduced at 10 and 21 d. IGF binding protein (IGFBP)-1 hepatic expression was elevated at 10 d (P < 0.001) but not at 21 d. Adult LP-con offspring had reduced body weight (P < 0.05), lean (P < 0.0001) and bone (P < 0.0001) but not fat (P = 0.6) mass with no persistent effects on IGF-I, ALS and IGFBP-1.Postnatal lp feeding reduced lean mass (P < 0.0001) and bone mass (P < 0.0001) in CON and LP animals. Percentage fat (LP P = 0.04; CON P = 0.6) and IGFBP-1 (LP P = 0.01; CON P = 0.2) were increased in LP-lp but not CON-lp offspring. This suggests that postnatal nutrition is important in the effects of maternal protein restriction on adult body composition and that IGFBP-1 may be involved.
Gosby
Alison K
AK
Human Nutrition Unit, The University of Sydney, NSW, Australia. alison.gosby@usyd.edu.au
Stanton
Lisa M L
LM
Maloney
Christopher A
CA
Thompson
Madeleine
M
Briody
Julie
J
Baxter
Robert C
RC
Bryson
Janet M
JM
Denyer
Gareth S
GS
Caterson
Ian D
ID
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development* / Q000378:metabolism; D001823:Body Composition; D001835:Body Weight; D002352:Carrier Proteins / Q000097:blood; D018753:Diet, Protein-Restricted; D005260:Female; D006023:Glycoproteins / Q000097:blood; D007328:Insulin / Q000097:blood; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007774:Lactation / Q000502:physiology; D008099:Liver / Q000737:chemistry; D039382:Maternal Nutritional Physiological Phenomena; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014886:Weaning
D002352:Carrier Proteins; D006023:Glycoproteins; D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; C075944:insulin-like growth factor binding protein, acid labile subunit; D007334:Insulin-Like Growth Factor I