Distal IgA immunity can be sustained by alphaEbeta7+ B cells in L-selectin-/- mice following oral immunization.
Understanding the role of homing receptors could aid vaccine strategies for developing distal mucosal immunity. Infection studies have revealed that immune intestinal B cells use alpha(4)beta(7) homing receptors, but their role in subsequent oral immunization with soluble antigens is unknown. To assess the influence of L-selectin and alpha(4)beta(7) on distal B cells following oral cholera toxin (CT) immunization, L-selectin-deficient (L-Sel(-/-)) IgA anti-CT-B-specific B cells were enhanced 30-, 9.2-, and 3.5-fold in head and neck lymph nodes (HNLNs), nasal-associated lymphoid tissue, and nasal passages (NPs), respectively, vs. L-Sel(+/+) mice. Cell-sorted intestinal and NP IgA antibody-forming cells (AFCs) were mostly alpha(4)beta(7)(+), unlike HNLN L-Sel(-/-) IgA and IgG anti-CT-B AFCs that were alpha(E)beta(7)(+), contrasting with L-Sel(+/+) HNLN IgA AFCs that were mostly alpha(4)beta(7)(+). In vitro studies revealed that L-Sel(-/-) HNLN B cells preferentially expressed alpha(E) following polyclonal stimulation. These studies show that HNLN B cells express alpha(E)beta(7) in the absence of L-selectin to sustain distal IgA responses.
Pascual
D W
DW
Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, USA. dpascual@montana.edu
Riccardi
C
C
Csencsits-Smith
K
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology / Q000494:pharmacology*; D005260:Female; D007114:Immunization; D007070:Immunoglobulin A / Q000235:genetics / Q000276:immunology*; D016023:Integrins / Q000235:genetics / Q000276:immunology*; D019041:L-Selectin / Q000235:genetics / Q000276:immunology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016201:Receptors, Lymphocyte Homing / Q000235:genetics / Q000276:immunology*; D014612:Vaccines / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D007070:Immunoglobulin A; D016023:Integrins; D016201:Receptors, Lymphocyte Homing; D014612:Vaccines; C070709:integrin alphaEbeta7; D019041:L-Selectin; D002772:Cholera Toxin