title: Accumulation of the bioactive peptides, novokinin, LPYPR and rubiscolin, in seeds of genetically modified soybean.
abstract: Novokinin (RPLKPW), LPYPR, and rubiscolin (YPLDLF) are bioactive peptides with respective hypotensive, hypocholesterolemic, and memory-enhancing activities. We generated transgenic soybean lines that expressed modified forms of the alpha' subunit of seed storage protein beta-conglycinin containing tandem repeats of these bioactive peptides. The modified alpha' subunits constituted up to 0.2% of extracted proteins from the transgenic seeds.
authors.affiliation: National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido, Japan.
authors.forename: Keito
authors.initials: K
authors.lastname: Nishizawa
authors.forename: Akiko
authors.initials: A
authors.lastname: Kita
authors.forename: Chieko
authors.initials: C
authors.lastname: Doi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Kousaku
authors.initials: K
authors.lastname: Ohinata
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Masao
authors.initials: M
authors.lastname: Ishimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D030841:Food, Genetically Modified; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism*
chemical_list: C511871:Arg-Pro-Leu-Lys-Pro-Trp; D009842:Oligopeptides