title: Recombinant protein secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a novel signal peptide.
abstract: Secretion of recombinant proteins aims to reproduce the correct posttranslational modifications of the expressed protein while simplifying its recovery. In this study, secretion signal sequences from an abundantly secreted 34-kDa protein (P34) from Pseudozyma flocculosa were cloned. The efficiency of these sequences in the secretion of recombinant green fluorescent protein (GFP) was investigated in two Pseudozyma species and compared with other secretion signal sequences, from S. cerevisiae and Pseudozyma spp. The results indicate that various secretion signal sequences were functional and that the P34 signal peptide was the most effective secretion signal sequence in both P. flocculosa and P. antarctica. The cells correctly processed the secretion signal sequences, including P34 signal peptide, and mature GFP was recovered from the culture medium. This is the first report of functional secretion signal sequences in P. flocculosa. These sequences can be used to test the secretion of other recombinant proteins and for studying the secretion pathway in P. flocculosa and P. antarctica.
authors.affiliation: Department of Phytology, Horticulture Research Centre, Envirotron, University Laval, Quebec, Canada.
authors.forename: Yali
authors.initials: Y
authors.lastname: Cheng
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Avis
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bolduc
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Raphaël
authors.initials: R
authors.lastname: Anguenot
authors.forename: Bertrand
authors.initials: B
authors.lastname: Neveu
authors.forename: Caroline
authors.initials: C
authors.lastname: Labbé
authors.forename: François
authors.initials: F
authors.lastname: Belzile
authors.forename: Richard R
authors.initials: RR
authors.lastname: Bélanger
mesh_terms: D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D017930:Genes, Reporter; D016681:Genome, Fungal; D023281:Genomics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014587:Ustilaginales / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins