title: An unambiguous structural elucidation of a 1,3-beta-D-glucan obtained from liquid-cultured Grifola frondosa by solution NMR experiments.
abstract: Grifolan LE (GRN-LE), a purified beta-D-glucan, which is obtained from liquid-cultured Grifola frondosa, exhibits various biological activities, including antitumor effects. Significant progress has been made in the study of these effects. However, an unambiguous structural characterization of GRN-LE using NMR spectroscopy has not been carried out as yet. It is well accepted that the biological effects of a beta-glucan depend on its primary structure, conformation, and molecular weight. In the present study, we unambiguously elucidate the primary structure of GRN-LE using NMR spectroscopy. The data presented here reveal that GRN-LE comprises a 1,3-beta-D-glucan backbone with a single 1,6-beta-D-glucosyl side branching unit on every third residue.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Tada
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D002478:Cells, Cultured; D005936:Glucans / Q000737:chemistry*; D048691:Grifola / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D012996:Solutions / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D005936:Glucans; D008024:Ligands; D012996:Solutions; D047071:beta-Glucans; C539908:grifolan LE