Enumeration of bacteriophages using the small drop plaque assay system.
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.
Mazzocco
Amanda
A
Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
Waddell
Thomas E
TE
Lingohr
Erika
E
Johnson
Roger P
RP
D001435:Bacteriophages / Q000254:growth & development* / Q000302:isolation & purification*; D010948:Viral Plaque Assay / Q000379:methods*