Amplified fluorescence determination of microRNAs in homogeneous solution with cationic conjugated polymers.
Combining the miRNA-primed extension reaction and the optical amplification of a conjugated polymer, a sensitive, selective and homogeneous method for miRNA determination has been developed based on a fluorescence resonance energy transfer (FRET) mechanism.
Zhang
Yali
Y
Hebei University, Baoding, 071002, Hebei Province, People's Republic of China. .
Li
Zhengping
Z
Cheng
Yongqiang
Y
D001483:Base Sequence; D002412:Cations; D015342:DNA Probes; D031541:Fluorescence Resonance Energy Transfer; D035683:MicroRNAs / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002412:Cations; D015342:DNA Probes; D035683:MicroRNAs; D011108:Polymers