title: Template usage is responsible for the preferential acquisition of the K65R reverse transcriptase mutation in subtype C variants of human immunodeficiency virus type 1.
abstract: We propose that a nucleotide template-based mechanism facilitates the acquisition of the K65R mutation in subtype C human immunodeficiency virus type 1 (HIV-1). Different patterns of DNA synthesis were observed using DNA templates from viruses of subtype B or C origin. When subtype C reverse transcriptase (RT) was employed to synthesize DNA from subtype C DNA templates, preferential pausing was seen at the nucleotide position responsible for the AAG-to-AGG K65R mutation. This did not occur when the subtype B RT and template were used. Template factors can therefore increase the probability of K65R development in subtype C HIV-1.
authors.affiliation: McGill University AIDS Center, McGill University, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Canada.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Coutsinos
authors.forename: Cédric F
authors.initials: CF
authors.lastname: Invernizzi
authors.forename: Hongtao
authors.initials: H
authors.lastname: Xu
authors.forename: Daniela
authors.initials: D
authors.lastname: Moisi
authors.forename: Maureen
authors.initials: M
authors.lastname: Oliveira
authors.forename: Bluma G
authors.initials: BG
authors.lastname: Brenner
authors.forename: Mark A
authors.initials: MA
authors.lastname: Wainberg
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D054303:HIV Reverse Transcriptase / Q000235:genetics* / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D020125:Mutation, Missense; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase