title: Vinegar as an antimicrobial agent for control of Candida spp. in complete denture wearers.
abstract: The use of denture is known to increase the carriage of Candida in healthy patients, and the proliferation of Candida albicans strains can be associated with denture-induced stomatitis. The aim of this study was to evaluate the use of vinegar as an antimicrobial agent for control of Candida spp. in complete upper denture wearers. Fifty-five patients were submitted to a detailed clinical interview and oral clinical examination, and were instructed to keep their dentures immersed in a 10% vinegar solution (pH less than 3) overnight for 45 days. Before and after the experimental period, saliva samples were collected for detection of Candida, counting of cfu/mL and identification of species by phenotypical tests (germ tube formation, chlamidoconidia production, and carbohydrate fermentation and assimilation). The results were analyzed using Spearman's correlation and Student's t-test (p<or=0.05). Candida yeasts were present in 87.3% of saliva samples before the treatment. A significant reduction was verified in CFU/mL counts of Candida after treatment. A positive correlation between Candida and denture stomatitis was verified, since the decrease of cfu/mL counts was correlated with a reduction in cases of denture stomatitis. Although it was not able to eliminate C. albicans, the immersion of the complete denture in 10% vinegar solution, during the night, reduced the amounts (cfu/mL) of Candida spp. in the saliva and the presence of denture stomatitis in the studied patients.
authors.affiliation: Department of Dentistry, University of Taubaté, Taubaté, SP, Brazil.
authors.forename: Telma Maria Silva
authors.initials: TM
authors.lastname: Pinto
authors.forename: Ana Christina Claro
authors.initials: AC
authors.lastname: Neves
authors.forename: Mariella Vieira Pereira
authors.initials: MV
authors.lastname: Leão
authors.forename: Antonio Olavo Cardoso
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D019342:Acetic Acid / Q000627:therapeutic use*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification; D041221:Candida glabrata / Q000187:drug effects / Q000302:isolation & purification; D041022:Candida tropicalis / Q000187:drug effects / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003820:Denture Cleansers / Q000627:therapeutic use; D003827:Denture, Complete, Upper / Q000382:microbiology*; D005500:Follow-Up Studies; D006801:Humans; D007101:Immersion; D008875:Middle Aged; D012463:Saliva / Q000382:microbiology; D013282:Stomatitis, Denture / Q000382:microbiology / Q000517:prevention & control*; D014099:Toothbrushing / Q000295:instrumentation
chemical_list: D000891:Anti-Infective Agents, Local; D003820:Denture Cleansers; D019342:Acetic Acid