title: [Anti-tumor mechanism of active components from extract of Actinidia rufa root].
abstract: OBJECTIVE
  To observe effect and mechanism of n-Butanol lysate of alcohol extracts from Actinidia rufa root (monomer of R6,R8).
  
  
  METHODS
  Tunel, Wright's stain with Giemsa's stain dyeing, and Hoechst 33258-PI double dyeing assay were used to detect the apoptosis of SGC7901 tumor cells treated with R6, R8. The SGC7901 tumor cells were randomly divided into control group and two treatment groups administered 0.05 g x L(-1) R6, R8, respectively, for 72 h). FCM assay was used to detect the apoptosis. Agarose electrophoresis assay was used to detect DNA strand break of tumor cells and reveal anti-tumor action mechanism.
  
  
  RESULTS
  The apoptosis percentage of the tumor cell in 24 h, 48 h, 72 h was (17.08 +/- 2.78)% , (29.68 +/- 2.96)%, (52.46 +/- 3.81)%; (14.75 +/- 2.14)%, (27.35 +/- 3.79)%, (45.64 +/- 5.24)%, respectively, for the treatment group, significantly higher than that in the control group (1.94 +/- 1.55)%, (2.78 +/- 1.84)%, (11.8 +/- 2.79)% (P < 0.01) by tunnel assay. Wright's stain with Giemsa's stain dyeing assay, Hoechst 33258-PI and FCM double dyeing assay showed same action. R6 and R8 had the effect of inducing the DNA histogram of tumor cells (P < 0.01).
  
  
  CONCLUSIONS
  The anti-tumor mechanisms may be associated with inducing the injury of DNA and stimulating apoptosis.
authors.affiliation: Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Guo-biao
authors.initials: GB
authors.lastname: Lin
authors.forename: Zhen-guo
authors.initials: ZG
authors.lastname: Zhong
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Zhang
authors.forename: Feng-fen
authors.initials: FF
authors.lastname: Zhang
authors.forename: Xi-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Chu-sheng
authors.initials: CS
authors.lastname: Huang
mesh_terms: D029042:Actinidia / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal