Thirty-five percent oxygen pre-conditioning protects PC12 cells against death induced by hypoxia.
The present study is designed to investigate the effect of pre-conditioning with 35% O2 on PC12 cell death induced by hypoxia. This study investigated whether 35% O2 pre-conditioning for 3 h, followed by 12 h recovery, can protect PC12 cells against death induced by subsequent exposure to hypoxia for 72 h. The result showed that pre-conditioning with 35% O2 partly blocked the decrease in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction induced by hypoxia in PC12 cells. PC12 cells pre-conditioned with 35% O2 could generate a small quantity of reactive oxygen species (ROS), which activated the extracellular signal-regulated kinase (ERK) signalling pathway, then the over-expression of the B-cell lymphoma/leukaemia-2 (Bcl-2) was induced, which subsequently protected PC12 cell against death resulting from hypoxia exposure. In conclusion, 35% O2 pre-conditioning could protect PC12 cells against hypoxic insult.
Cao
Zhongping
Z
Department of Pathophysiology and High Altitude Physiology, Key Laborary of High Aititude Medicine of Educative Ministry, The Third Military Medical University, Chongqing, PR China.
Gao
Wenxiang
W
Tao
Guocai
G
Fan
Youming
Y
Liu
Fuyu
F
Gao
Yuqi
Y
D000818:Animals; D015153:Blotting, Western; D015687:Cell Hypoxia / Q000187:drug effects*; D019610:Cytoprotection / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019194:Ischemic Preconditioning / Q000379:methods*; D010100:Oxygen / Q000494:pharmacology*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D048049:Extracellular Signal-Regulated MAP Kinases; D010100:Oxygen