Phylogenetic analysis of parapoxviruses and the C-terminal heterogeneity of viral ATPase proteins.
Two outbreaks of orf virus (a parapoxvirus) infection in goats found in Nantou and Taiping of central Taiwan were investigated. The nucleotide and the amino acid sequences of viral B2L, E3L and A32L genes in these two outbreaks were analyzed, and each of their phylogenetic trees were also constructed. In the A32L gene, an unexpected deletion of 24 nucleotides was found in the Taiping strain. The A32L gene can encode an ATPase and is supposed to be involved in virion DNA packaging. The 24 nucleotides correspond to 8 amino acids residues of the viral ATPase, which are located near the C-terminal region of the enzyme. Moreover, two copies of the RGD sequence at C-terminal region of ATPase were found in the Nantou strain. The 24-nucleotide difference in the A32L gene indicated that the Nantou strain and the Taiping strain were two separate strains, and it can be used in differential molecular diagnosis. Moreover, the C-terminal heterogeneity was found to be a general feature of the viral ATPase. Lastly, similar functional motifs of the ATPase and the Ras proto-oncoprotein (a GTPase) are discussed.
Chan
Kun-Wei
KW
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan.
Yang
Cheng-Hsiung
CH
Lin
Jen-Wei
JW
Wang
Hsien-Chi
HC
Lin
Fong-Yuan
FY
Kuo
Shu-Ting
ST
Wong
Min-Liang
ML
Hsu
Wei-Li
WL
D000251:Adenosine Triphosphatases / Q000737:chemistry; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D004196:Disease Outbreaks; D005814:Genes, Viral; D008969:Molecular Sequence Data; D018157:Parapoxvirus / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure; D010802:Phylogeny; D011213:Poxviridae Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D013624:Taiwan / Q000453:epidemiology; D014764:Viral Proteins / Q000737:chemistry; D006041:Goats; D000818:Animals
D000251:Adenosine Triphosphatases; D014764:Viral Proteins