Presence of HPV type 6 in dysplasia and carcinoma arising from recurrent respiratory papillomatosis.
BACKGROUND
We collected rare cases of recurrent respiratory papillomatosis (RRP) undergoing malignant transformation. We sought to identify human papillomavirus (HPV) subtypes in areas of papilloma, dysplasia, and carcinoma and investigate the pattern of protein overexpression.


METHODS
Three patients whose disease underwent malignant transformation from RRP to carcinoma were subjected to this study. Morphologically distinct areas in the pathology specimen of each patient were diagnosed as papilloma, dysplasia, and carcinoma. Each lesion was separately obtained by laser capture microdissection and was PCR amplified for the presence of HPV. A DNA chip was used to determine the type of HPV in each area. Immunohistochemistry for p53, Ki-67, and pRb was performed.


RESULTS
HPV type 6 was present in all specimens tested positive. Expression of p53 and Ki-67 increased with increasing severity of dysplastic change.


CONCLUSIONS
Although HPV type 11 is most frequently associated with malignant change of RRP, HPV type 6 may also contribute to play an equally important role in RRP carcinogenesis.
Jeong
Woo-Jin
WJ
Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, Korea.
Park
Seok-Woo
SW
Shin
Miwha
M
Lee
You Jeong
YJ
Jeon
Yoon Kyung
YK
Jung
Young Ho
YH
Hah
J Hun
JH
Kwon
Tack-Kyun
TK
Song
Yong-Sang
YS
Kim
Kwang Hyun
KH
Sung
Myung-Whun
MW
D000328:Adult; D001707:Biopsy, Needle; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D018450:Disease Progression; D052163:Human papillomavirus 6 / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D010212:Papilloma / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000473:pathology*; D011379:Prognosis; D012189:Retrospective Studies; D018570:Risk Assessment; D012494:Sampling Studies