[A novel transcriptional regulator of the phzA1 operon in Pseudomonas aeruginosa].
OBJECTIVE
The aim was to investigate the regulator of phzA1B1C1D1E1F1(phzA1) operon in Pseudomonas aeruginosa.


METHODS
Mutants affecting phzA1 expression in the presence of subinhibitory concentrations of tetracyclin were selected by screening a transposon mutagenesis library. The transposon insertion sites in the mutants were determined by arbitrary PCR and subsequent PCR product sequencing.


RESULTS
We found two mutants affecting the phzA1 operon expression. The transposon insertion sites in these two mutants were at the promoter region of gene PA0487 encoding a probable molybdenum transport regulator. Some genes involved in quorum sensing were also down-regulated in the mutant.


CONCLUSIONS
PA0487 was involved in regulating the expression of phzA1, possibly through the quorum sensing system.
Liping
Kong
K
Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China. kongliping0516@163.com
Haihua
Liang
L
Zhaolin
Dong
D
Kangmin
Duan
D
Lixin
Shen
S
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016254:Mutagenesis, Insertional; D009876:Operon; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D053038:Quorum Sensing; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D014157:Transcription Factors