Towards [Cp*Rh(bpy)(H2O]2+-promoted P450 catalysis: direct regeneration of CytC.
The organometallic complex pentamethylcyclopentadienyl rhodium 2,2'-bipyridin ([Cp(*)Rh(bpy)(H(2)O)](2+)) was applied as regeneration catalyst for cytochrome C (CytC). Direct reduction of CytC-bound Fe(III) was achieved in this model system pointing towards a potential usefulness of this concept to promote cell-free P450 catalysis. In addition, controlled in situ provision with hydrogen peroxide was performed using [Cp(*)Rh(bpy)(H(2)O)](2+) resulting in improved CytC-catalyzed sulfoxidation of thioanisol This work represents the first step towards the direct-[Cp(*)Rh(bpy)(H(2)O)](2+) catalyzed regeneration of P450 monooxygenases and peroxidases.
Hollmann
Frank
F
Institute of Biotechnology, Swiss Federal Institute of Technology, Zurich, Switzerland. f.hollmann@tudelft.nl
Schmid
Andreas
A
D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D009942:Organometallic Compounds / Q000494:pharmacology*
C476958:(2,2'-bipyridyl)(pentamethylcyclopentadienyl)rhodium; D009942:Organometallic Compounds; D045304:Cytochromes c; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide