A new medium for diagnosis of dermatophyte infection.
A new medium (DBM) was compared with dermatophyte test medium (DTM) for the diagnosis of dermatophyte infection. The sensitivity was 103 cfu/mL (2 x 101 cfu/slant) for both DTM and DBM with a suspension of Trichophyton rubrum. In axenic cultures, all dermatophytes tested altered the color of both media. Although most non dermatophytic molds made a color change, it was at a slower rate. In nail samples of dermatophyte infection, all dermatophytes altered the color of both media. However, the time for discoloration was shorter with DBM than with DTM (5.83 +/- 0.39 days vs. 7.32 +/- 0.41 days, t = 2.63, P = 0.01). Most isolates of nondermatophyte also made a discoloration, but they could be distinguished from dermatophytes by their colonial diameters when the color began to change (> or = 5 mm). Our results were in good agreement with a professional laboratory of medical mycology, however, the latter is regularly able to differentiate exactly the species of the growing dermatophyte. The DBM medium is more convenient, rapid, more accurate and economical to use than DTM.
Li
Xiao-Fang
XF
Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Jiang Wangmiao jie 14, Nanjing 210042, China.
Shen
Yong-Nian
YN
Chen
Wei
W
Chen
Hui
H
Lv
Gui-Xia
GX
Liu
Wei-Da
WD
D000704:Analysis of Variance; D003883:Arthrodermataceae / Q000145:classification*; D016009:Chi-Square Distribution; D003470:Culture Media; D003881:Dermatomycoses / Q000175:diagnosis*; D006801:Humans; D007202:Indicators and Reagents; D016533:Mycological Typing Techniques / Q000379:methods*; D014009:Onychomycosis / Q000175:diagnosis; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D003470:Culture Media; D007202:Indicators and Reagents