title: Antimicrobial susceptibility of monoculture biofilms of a clinical isolate of Enterococcus faecalis.
abstract: The purpose of this study was to create a monoculture biofilm of a clinical isolate of Enterococcus faecalis and to determine susceptibility against four antimicrobial irrigants. Biofilms were subjected to 1-, 3-, and 5-minute exposures to one of the following irrigants: 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX) or one of two new products, <6% NaOCl with surface modifiers (Chlor-XTRA) or 2% CHX with surface modifiers (CHX-Plus) (Vista Dental Products, Racine, WI). It was hypothesized that NaOCl and CHX would be equally effective and that addition of surface modifiers would improve bactericidal activity of the respective irrigants compared to the original formulations. Results indicate that 6% NaOCl and Chlor-EXTRA were significantly superior against E. faecalis biolfilms compared to 2% CHX and CHX-Plus at all time points except five minutes.
authors.affiliation: Department of Endodontics, University of Iowa College of Dentistry, Iowa City, Iowa, USA. anne-williamson@uiowa.edu
authors.forename: Anne E
authors.initials: AE
authors.lastname: Williamson
authors.forename: Jared W
authors.initials: JW
authors.lastname: Cardon
authors.forename: David R
authors.initials: DR
authors.lastname: Drake
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology; D013293:Enterococcus faecalis / Q000187:drug effects*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology; D012388:Root Canal Irrigants / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants