title: [Effect of different choices and treatments with donor cells on reprogramming].
abstract: The donor nucleus must experienced the epigenetic modification of the process reprogramming and went back to the initial state after the donor cell was injected into the oocytes. If the reprogramming is not completed, the efficiency of cloning will be reduced. However, reprogramming of nucleus muct was not only embodied in its ability after it was transferred into the oocytes. It was different in the potential if the cell type was not identical. In addition, different treatment to the donor cells resulted in different ability and the level of reprogramming. This paper described different effects of the type, algebra, cycles, age, and species of the donor cells after nuclear transplantation on the reprogramming. An overview of the exposition and analysis through the donor cell cryopreservation, serum starvation, and different reagent treatments were discussed.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China.
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Yao
authors.forename: Xiang-Chen
authors.initials: XC
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Qiao
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Guan
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Ma
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002477:Cells / Q000166:cytology* / Q000378:metabolism; D065150:Cellular Reprogramming; D016922:Cellular Senescence; D015925:Cryopreservation; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism
chemical_list: D007202:Indicators and Reagents