A cathepsin L-like protease from Strongylus vulgaris: an orthologue of Caenorhabditis elegans CPL-1.
Cathespin L-like proteases (CPLs), characterized from a wide range of helminths, are significant in helminth biology. For example, in Caenorhabditis elegans CPL is essential for embryogenesis. Here, we report a cathepsin L-like gene from three species of strongyles that parasitize the horse, and describe the isolation of a cpl gene (Sv-cpl-1) from Strongylus vulgaris, the first such from equine strongyles. It encodes a protein of 354 amino acids with high similarity to other parasitic Strongylida (90-91%), and C.elegans CPL-1 (87%), a member of the same Clade. As S.vulgaris cpl-1 rescued the embryonic lethal phenotype of the C.elegans cpl-1 mutant, these genes may be orthologues, sharing the same function in each species. Targeting Sv-CPL-1 might enable novel control strategies by decreasing parasite development and transmission.
Ultaigh
Sinéad Nic An
SN
School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
Carolan
James C
JC
Britton
Collette
C
Murray
Linda
L
Ryan
Michael F
MF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics*; D056668:Cathepsin L; D002403:Cathepsins / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D006736:Horses; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D018510:RNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D013323:Strongylus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth; D002403:Cathepsins; C466023:CPL-1 protein, C elegans; D056668:Cathepsin L