Crystallization and preliminary crystallographic analysis of laminarinase from Rhodothermus marinus: a case of pseudomerohedral twinning.
Thermophilic endo-1,3(4)-beta glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method. The needle-like crystals belong to space group P2(1) and contain two protein molecules in the asymmetric unit with a solvent content of 51.75 %. Diffraction data were collected to a resolution of 1.95A and resulted in a dataset with an overall R(merge) of 10.4% and a completeness of 97.8%. Analysis of the structure factors revealed pseudomerohedral twinning of the crystals with a twin fraction of approximately 42%.
Golubev
Alexander M
AM
Petersburg Nuclear Physics Institute, Gatchina, St. Petersbug 188300, Rusia.
Rojas
Adriana L
AL
Nascimento
Alessandro S
AS
Bleicher
Lucas
L
Kulminskaya
Anna A
AA
Eneyskaya
Elena V
EV
Polikarpov
Igor
I
D003460:Crystallization; D018360:Crystallography, X-Ray; D044644:Endo-1,3(4)-beta-Glucanase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D009154:Mutation; D045245:Rhodothermus / Q000201:enzymology*
D044644:Endo-1,3(4)-beta-Glucanase