A SNP transferability survey within the genus Vitis.
BACKGROUND
Efforts to sequence the genomes of different organisms continue to increase. The DNA sequence is usually decoded for one individual and its application is for the whole species. The recent sequencing of the highly heterozygous Vitis vinifera L. cultivar Pinot Noir (clone ENTAV 115) genome gave rise to several thousand polymorphisms and offers a good model to study the transferability of its degree of polymorphism to other individuals of the same species and within the genus.


RESULTS
This study was performed by genotyping 137 SNPs through the SNPlex Genotyping System (Applied Biosystems Inc.) and by comparing the SNPlex sequencing results across 35 (of the 137) regions from 69 grape accessions. A heterozygous state transferability of 31.5% across the unrelated cultivars of V. vinifera, of 18.8% across the wild forms of V. vinifera, of 2.3% among non-vinifera Vitis species, and of 0% with Muscadinia rotundifolia was found. In addition, mean allele frequencies were used to evaluate SNP informativeness and develop useful subsets of markers.


CONCLUSIONS
Using SNPlex application and corroboration from the sequencing analysis, the informativeness of SNP markers from the heterozygous grape cultivar Pinot Noir was validated in V. vinifera (including cultivars and wild forms), but had a limited application for non-vinifera Vitis species where a resequencing strategy may be preferred, knowing that homology at priming sites is sufficient. This work will allow future applications such as mapping and diversity studies, accession identification and genomic-research assisted breeding within V. vinifera.
Vezzulli
Silvia
S
IASMA Research Centre, Via E, Mach 1, 38010 San Michele all'Adige (TN), Italy. silvia.vezzulli@iasma.it
Micheletti
Diego
D
Riaz
Summaira
S
Pindo
Massimo
M
Viola
Roberto
R
This
Patrice
P
Walker
M Andrew
MA
Troggio
Michela
M
Velasco
Riccardo
R
D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant; D005838:Genotype; D006579:Heterozygote; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods; D027843:Vitis / Q000235:genetics*
D018744:DNA, Plant