title: Constitutive expression of a rice GTPase-activating protein induces defense responses.
abstract: G-proteins (guanine nucleotide-binding proteins that usually exhibit GTPase activities) and related signal transduction processes play important roles in mediating plant defense responses; here, a rice (Oryza sativa) cDNA clone, OsGAP1, encoding a GTPase-activating protein (GAP) that also contains a protein kinase C conserved region 2 (C2) domain is reported. An interacting G-protein partner for the OsGAP1 protein was identified by yeast two-hybrid library screening and confirmed by co-immunoprecipitation; the GTPase-activation activity of OsGAP1 on this interacting G-protein was demonstrated using in vitro assays. OsGAP1 was induced by wounding in rice and the presence of the R locus Xa14 enhances such induction. Gain-of-function tests in transgenic rice and Arabidopsis thaliana showed that constitutive expression of OsGAP1 led to increased resistance to bacterial pathogens in both monocots and dicots.
authors.affiliation: Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Cheung
authors.affiliation: Department of Biology and.
authors.forename: Nai-Yan
authors.initials: NY
authors.lastname: Zeng
authors.affiliation: Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Suk-Wah
authors.initials: SW
authors.lastname: Tong
authors.affiliation: Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Wing-Yen Francisca
authors.initials: WF
authors.lastname: Li
authors.affiliation: State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Kai-Jun
authors.initials: KJ
authors.lastname: Zhao
authors.affiliation: Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Chunlian
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: China National Rice Research Institute, Hangzhou, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Fu
authors.affiliation: China National Rice Research Institute, Hangzhou, China.
authors.forename: Zongxiu
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Samuel Sai-Ming
authors.initials: SS
authors.lastname: Sun
authors.affiliation: Department of Biology and.|State (China) Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.
authors.forename: Hon-Ming
authors.initials: HM
authors.lastname: Lam
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism; D003001:Cloning, Molecular; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism*; D010935:Plant Diseases; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D020690:GTPase-Activating Proteins; D010940:Plant Proteins