title: Metabonomics and population studies: age-related amino acids excretion and inferring networks through the study of urine samples in two Italian isolated populations.
abstract: The study of two different Italian isolated populations was combined with a metabonomic approach to better understand tubular handling of amino acids. Levels of amino acids and metabolites have been analyzed by Nucleic Magnetic Resonance and expressed as ratio vs urinary creatinine concentration (mmol/mol). For most of the amino acids there is an age-related U shape pattern of excretion, with the peaks during childhood and old age, and a significant reduction in the adult age. Hierarchical cluster analysis has clearly identified three groups clustering the same amino acids: His, Thr and Ala (group one); Gly and Phe (group two) and a third larger one. Results have been further confirmed by factor and regression analysis, and used to confirm and, in some cases, infer new amino acids networks. As a matter of facts, the identification of strong evidences for clustering of urine excretion of several neutral amino acids suggests the predominant impact of relevant and common transporters.
authors.affiliation: Medical Genetics, Department of Laboratory Medicine, Institute for Maternal and Child Health IRCCS-Burlo Garofolo, Trieste, Italy.
authors.forename: Pio
authors.initials: P
authors.lastname: D'Adamo
authors.forename: Sheila
authors.initials: S
authors.lastname: Ulivi
authors.forename: Amerigo
authors.initials: A
authors.lastname: Beneduci
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pontoni
authors.forename: Giovambattista
authors.initials: G
authors.lastname: Capasso
authors.forename: Carmela
authors.initials: C
authors.lastname: Lanzara
authors.forename: Gilberto
authors.initials: G
authors.lastname: Andrighetto
authors.forename: Uros
authors.initials: U
authors.lastname: Hladnik
authors.forename: Virginia
authors.initials: V
authors.lastname: Nunes
authors.forename: Manuel
authors.initials: M
authors.lastname: Palacin
authors.forename: Paolo
authors.initials: P
authors.lastname: Gasparini
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000596:Amino Acids / Q000737:chemistry / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007558:Italy; D008297:Male; D055432:Metabolomics; D008875:Middle Aged; D044382:Population Groups; D012424:Rural Population; D055815:Young Adult
chemical_list: D000596:Amino Acids