title: Peripheral nerve lesion induces an up-regulation of Spy1 in rat spinal cord.
abstract: Spy1, as a member of the Speedy/RINGO family and a novel activator of cyclin-dependent kinases, was shown to promote cell cycle progression and cell survival in response to DNA damage. While its expression and roles in nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve injury model in adult rats and studied the dynamic changes of Spy1 expression in lumbar spinal cord. Temporally, Spy1 expression was increased shortly after sciatic nerve crush and peaked at day 2. Spatially, Spy1 was widely expressed in the lumbar spinal cord including neurons and glial cells. While after injury, Spy1 expression was increased predominantly in astrocytes and microglia, which were largely proliferated. Moreover, there was a concomitant up-regulation of CDK2 activity and down-regulation of p27. Collectively, we hypothesized peripheral nerve injury induced an up-regulation of Spy1 in lumbar spinal cord, which was associated with glial proliferation.
authors.affiliation: Orthopaedics of the Second Affiliated Hospital, Nanjing Medical University, Nanjing, 226001, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Huang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.forename: Junling
authors.initials: J
authors.lastname: Yang
authors.forename: Mudan
authors.initials: M
authors.lastname: Lu
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Ke
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
authors.forename: Meijuan
authors.initials: M
authors.lastname: Yan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008159:Lumbar Vertebrae / Q000201:enzymology / Q000473:pathology; D008297:Male; D010641:Phenotype; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000201:enzymology / Q000473:pathology*; D013116:Spinal Cord / Q000201:enzymology / Q000473:pathology*; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D015415:Biomarkers; D018797:Cell Cycle Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D051357:Cyclin-Dependent Kinase 2