title: Naturally occurring Class A ss-lactamases from the Burkholderia cepacia complex.
abstract: Chromosomally encoded ss-lactamases from the Burkholderia cepacia complex species (formerly Pseudomonas cepacia) were characterized. Cloning and sequencing identified an Ambler class A ss-lactamase (PenB) from B. cenocepacia. It shares 82% amino acid identity with the PenA ss-lactamases previously identified from B. multivorans 249. Its expression was dependent upon a LysR-type regulatory protein. Its narrow-spectrum hydrolysis activity mostly included penicillins but also included expanded-spectrum cephalosporins and aztreonam at lower levels. In that study, Pen-like ss-lactamases (PenC, PenD, PenE, PenF) that shared 63 to 92% identity with PenB from B. cenocepacia were identified from other Burkholderia species. The corresponding ss-lactamase genes might be used as genetic tools for accurate Burkholderia species identification.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Le Kremlin-Bicêtre Cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: José-Manuel
authors.initials: JM
authors.lastname: Rodriguez-Martinez
authors.forename: Patrick
authors.initials: P
authors.lastname: Plésiat
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D042602:Burkholderia cepacia complex / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D006868:Hydrolysis; D007526:Isoelectric Point; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases