Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells.
This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As(5+), Cr(6+), Cd(2+), and Pb(2+)) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr(6+) exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr(6+) lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 microg-Cr/mL-medium (p<0.05), respectively, but showed no significant difference at higher Cr(6+)concentrations (2.0-5.0 microg-Cr/mL-medium).
Chou
I-Cheng
IC
Department of Environmental Engineering, National Cheng Kung University, Tainan, Taiwan.
Wu
Yee-Lin
YL
Wang
Lin-Chi
LC
Chang-Chien
Guo-Ping
GP
Lee
Hsinyu
H
D000818:Animals; D001151:Arsenic / Q000633:toxicity; D002104:Cadmium / Q000633:toxicity; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002857:Chromium / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D007854:Lead / Q000633:toxicity; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008670:Metals / Q000633:toxicity*; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D014162:Transfection
D008670:Metals; D000072317:Polychlorinated Dibenzodioxins; D002104:Cadmium; D002857:Chromium; D007854:Lead; D008156:Luciferases; D001151:Arsenic