A proteome reference map of Trichomonas vaginalis.
Trichomoniasis caused by Trichomonas vaginalis is the most common sexual transmitted infection in the world. The 170-MB genome of this protozoan contains 60,000 genes, the largest number of genes ever identified in protozoan. High-throughput expression sequenced tag analysis showed that at least 4,000 genes were expressed in the trophozoite stage. In the present study, we use two-dimensional electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis to profile, identify, and characterize proteins expressed in the trophozoite stage of T. vaginalis. A total of 247 spots representing 164 different proteins were identified. The identified proteins with known sequence or motif/domain homologies were further classified into groups according to their biological functions. Among them, proteins related to carbohydrate metabolism represented the most abundant category in the T. vaginalis proteome. This study presented the most extensive proteomic analysis of T. vaginalis to date and provided a reference proteome database for future comparative proteomic studies.
Huang
Kuo-Yang
KY
Molecular Regulation and Bioinformatics Laboratory, Department of Parasitology, Chang Gung University, 259 Wenhwa 1st. Road, Kweishan, Taoyuan 333, Taiwan.
Chien
Kuen-Yi
KY
Lin
Yin-Chun
YC
Hsu
Wei-Min
WM
Fong
I-Kai
IK
Huang
Po-Jung
PJ
Yueh
Yuan-Ming
YM
Gan
Richie Ruei-Chi
RR
Tang
Petrus
P
D000818:Animals; D030562:Databases, Protein / Q000592:standards*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020543:Proteome / Q000592:standards*; D040901:Proteomics; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012015:Reference Standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014246:Trichomonas vaginalis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D053058:Trophozoites / Q000254:growth & development* / Q000378:metabolism
D020543:Proteome; D015800:Protozoan Proteins