title: Human stem cell factor (SCF) is a heparin-binding cytokine.
abstract: Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmin, but not others. The binding of SCF was also inhibited with 0.55 M or higher concentrations of NaCl in the medium. These results suggested that a high content of all three sulfate groups in repeating disaccharide units is required for interaction with SCF. Furthermore, pre-immobilized SCF on F/P MP-coated plates significantly stimulated proliferation of a human erythroleukemia cell line.
authors.affiliation: Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Fumie
authors.initials: F
authors.lastname: Oonuma
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Mori
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Harada
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tagawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
mesh_terms: D004187:Disaccharides / Q000032:analysis; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding / Q000187:drug effects; D019089:Stem Cell Factor / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004187:Disaccharides; D019089:Stem Cell Factor; D006493:Heparin