Bioconjugation with stable luminescent lanthanide(III) chelates comprising pyridine subunits.
The use of long-lifetime emitting lanthanide(III) chelate labels or probes together with time-resolved fluorometry in detection provides a method to generate sensitive bioaffinity assays. However, the use of stable chelates demands very complicated optimization of the chelate structure. A great number of chelates have been synthesized, but usually, only the most prominent structures were then converted to corresponding biomolecule labeling reactants. This review covers the syntheses of luminescent lanthanide chelates comprising a pyridine subunit that allow solution and solid-phase bioconjugation.
Hovinen
Jari
J
PerkinElmer Life and Analytical Sciences, Turku Site, Turku, Finland. jari.hovinen@perkinelmer.com
Guy
Pamela M
PM
D002614:Chelating Agents / Q000138:chemical synthesis* / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D028581:Lanthanoid Series Elements / Q000138:chemical synthesis* / Q000737:chemistry; D049408:Luminescent Agents / Q000138:chemical synthesis* / Q000737:chemistry; D011725:Pyridines / Q000138:chemical synthesis* / Q000737:chemistry
D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D011725:Pyridines; C023666:pyridine