title: Localization of EFA6A, a guanine nucleotide exchange factor for ARF6, in spermatogenic cells of testes of adult mice.
abstract: ADP ribosylation factors (ARFs) of small GTPase are molecular switches regulating various membrane dynamics. Among them, ARF6 has recently been highlighted because of its function to facilitate the interaction between the cytoskeleton and the plasma membrane. Each ARFs has its preferable or even specific guanine nucleotide exchange factors (GEFs) as its activators. According to our previous RT-PCR analysis, EFA6A, a guanine nucleotide exchange factor for ARF6, was restrictedly expressed in the brain, retina and testis. Different from previous studies on neurons, EFA6A, a guanine nucleotide exchange factor for ARF6, was first shown to be localized intensely in nuclei of spermatocytes of adult mouse testes in the present immunohistochemical study. This suggests a possible involvement of EFA6A-ARF6 signaling in the karyokinesis and cytokinesis.
authors.affiliation: Department of Anatomy, School of Medicine, Akita University, 1-1-1 Hondo, Akita 010-8543, Japan. rysuzuki@gipc.akita-u.ac.jp
authors.forename: Ryoji
authors.initials: R
authors.lastname: Suzuki
authors.forename: Sachiko
authors.initials: S
authors.lastname: Saino-Saito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Toshimori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013090:Spermatocytes / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*
chemical_list: D000089982:ADP-Ribosylation Factor 6; D020662:Guanine Nucleotide Exchange Factors; D018809:Proliferating Cell Nuclear Antigen; C504474:Psd protein, mouse; D020727:ADP-Ribosylation Factors; C000715727:Arf6 protein, mouse