The Effects of radiation on the safety and protective efficacy of an attenuated Plasmodium yoelii sporozoite malaria vaccine.
We are developing a radiation attenuated Plasmodium falciparum sporozoite (PfSPZ) malaria vaccine. An important step was to determine the minimum dose of irradiation required to adequately attenuate each sporozoite. This was studied in the Plasmodium yoelii rodent model system. Exposure to 100 Gy completely attenuated P. yoelii sporozoites (PySPZ). Next we demonstrated that immunization of mice intravenously with 3 doses of 750 PySPZ that had received 200 Gy, double the radiation dose required for attenuation, resulted in 100% protection. These results support the contention that a radiation attenuated sporozoite vaccine for malaria will be safe and effective at a range of radiation doses.
Chattopadhyay
Rana
R
Sanaria Inc., 9800 Medical Center Drive, Rockville, MD 20850, United States.
Conteh
Solomon
S
Li
Minglin
M
James
Eric R
ER
Epstein
Judith E
JE
Hoffman
Stephen L
SL
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005260:Female; D007114:Immunization; D017780:Malaria Vaccines / Q000009:adverse effects / Q000276:immunology / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D010967:Plasmodium yoelii / Q000276:immunology*; D012449:Safety; D034101:Sporozoites / Q000276:immunology / Q000528:radiation effects*; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000276:immunology / Q000528:radiation effects
D017780:Malaria Vaccines; D014613:Vaccines, Attenuated