title: Phage proteomics: applications of mass spectrometry.
abstract: Current techniques in mass spectrometry (MS) allow sensitive and accurate identification of proteins thanks to the in silico availability of these protein sequences within databases. This chapter provides a short overview of MS techniques used in the identification of phage structural proteins and focuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. Such analyses provide an experimental examination of structural proteins and confirm genome-based gene predictions.
authors.affiliation: Department of Biosystems, Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Ceyssens
authors.forename: J
authors.initials: J
authors.lastname: Robben
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D014764:Viral Proteins