title: A transcriptomic analysis of the EK1.Br strain of human fibroblastoid keratocytes: the effects of growth, quiescence and senescence.
abstract: There is a growing need within ocular research for well-defined cellular models of normal corneal biology. To meet this need we created and partially characterised a standard strain of human fibroblastoid keratocytes (EK1.Br) and demonstrated that phenotypic changes occur within these cells with replicative senescence in vitro. Using Affymetrix HG-U133A oligonucleotide arrays, this paper reports both a comprehensive analysis of the transcriptome of EK1.Br in the growing, quiescent and senescent states and a comparison of that transcriptome with those of primary corneal endothelium, lung fibroblasts and dermal fibroblasts grown under identical conditions. Data mining shows (i) that EK1.Br retain the characteristic transcriptional fingerprint of keratocytes in vitro (ii) that this phenotype can be distinguished from those of other 'fibroblasts' by groups of highly differentially expressed genes and (iii) that senescence induces a distinct dedifferentiation phenomenon in EK1.Br. These findings are contextualised into the broader literature on replicative senescence and are supported with a web-accessible and fully searchable public-access database (www.madras.cf.ac.uk/cornea).
authors.affiliation: Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom.
authors.forename: David
authors.initials: D
authors.lastname: Kipling
authors.forename: Dawn L
authors.initials: DL
authors.lastname: Jones
authors.forename: S Kaye
authors.initials: SK
authors.lastname: Smith
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Giles
authors.forename: Katrin
authors.initials: K
authors.lastname: Jennert-Burston
authors.forename: Badr
authors.initials: B
authors.lastname: Ibrahim
authors.forename: Angela N P
authors.initials: AN
authors.lastname: Sheerin
authors.forename: Amy J C
authors.initials: AJ
authors.lastname: Evans
authors.forename: William
authors.initials: W
authors.lastname: Rhys-Willams
authors.forename: Richard G A
authors.initials: RG
authors.lastname: Faragher
mesh_terms: D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000502:physiology; D003315:Cornea / Q000378:metabolism*; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype