[Effects of six polysaccharides extracted from plants on the immunological cells of mice].
OBJECTIVE
To compare the immunoenhancement of six polysaccharides on the murine immunological cells in vitro.


METHODS
The immunoenhancement of the polysaccharide on the murine splenocytes were tested by MTt assay. The effects of polysaceharides on IgG secretion of spleen lymphocytes were detected by ELISA. The activation of peritoneal macrophages were tested by the method of neutral red phagocytosis assay. The phagocytic activity of spleen NK cells were detected by the method of LDH.


RESULTS
Lentinan and Ganoderan could promote spleen lymphocyte proliferation with ConA significantly. Astragalan, Ganoderan and Lentinan had stronger function in promoting the IgG secretion and proliferation of spleen lymphocyte with LPS. Lichenan and Astragalan could significantly enhance activation of peritoneal macrophages and splenic NK.


CONCLUSIONS
The immuno-regulation mechanism and effects of six polysaccharides were indivadually variable. The polysaccharide aiming at different effect targets could be combined into the most immunopotentiating agent by the most appropriate doses ratio.
Wang
Guoxiu
G
Department of Nutrition & Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150086, China. guoxiu945@163.com
Lin
Weiming
W
Zhao
Ruifang
R
Lin
Na
N
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D005260:Female; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D007912:Lentinan / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011124:Polyporaceae / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D011134:Polysaccharides; D007912:Lentinan