10000 times volume reduction for fluorescence correlation spectroscopy using nano-antennas.
We present an experimental and theoretical study of a new scheme for Near-Field Fluorescence Correlation Spectroscopy that, using the field enhancement by optical nanoantennas, allows the reduction of the observation volume 4 orders of magnitude below the diffraction limit. This reduction can be used in two different ways: to increase the sample concentration and to improve the spatial resolution of the dynamics under study. Our experimental results using individual gold nanoparticles and a 150 microM Rose Bengal solution in glycerol confirm the volume reduction.
Estrada
Laura C
LC
Departamento de Física, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina. lestrada@df.uba.ar
Aramendía
Pedro F
PF
Martínez
Oscar E
OE
D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008027:Light; D008962:Models, Theoretical; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*