title: Mannose-binding lectin gene and promoter polymorphism in visceral leishmaniasis caused by Leishmania infantum.
abstract: Visceral leishmaniasis is an infectious disease caused by various species of Leishmania and Leishmania infantum is known to be associated with VL in Iran. Different factors can consider risk factors for VL that some remain unknown. The aim of present study is to determine the distribution of the alleles ofmannose-binding lectin gene codon 52, 54, 57 and promoter variants H/L, X/Y, P and Q in confirmed VL patients while compares then with normal controls and seek correlation between these variants and confirmed VL patients. Fifty eight confirmed VL patients blood samples were compared with one hundred and twenty normal controls from Azarbaijan population of Iran. MBL genotypes were investigated by polymerase chain reaction and restriction fragment length polymorphism. Allelic and genotypic frequency of the polymorphism at promoters and genes didn't show statistical differences in patients and normal controls, but frequency of alleles with high MBL concentration in VL patients was higher than controls (p = 0.03). We can conclude that normal alleles with high MBL serum level are risk factor for VL and defective alleles have protective role in VL.
authors.affiliation: Department of Biochemistry and Laboratory Sciences, Faculty of Medicine, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Asgharzadeh
authors.forename: Abdolsamad
authors.initials: A
authors.lastname: Mazloumi
authors.forename: Hossein Samadi
authors.initials: HS
authors.lastname: Kafil
authors.forename: Ardavan
authors.initials: A
authors.lastname: Ghazanchaei
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D018314:Leishmania infantum / Q000472:pathogenicity*; D007898:Leishmaniasis, Visceral / Q000235:genetics* / Q000469:parasitology; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; D037601:Mannose-Binding Lectin