In vivo protective effect of crocetin on benzo(a)pyrene-induced lung cancer in Swiss albino mice.
The objective of this investigation was to determine the efficacy of crocetin in preventing lung tumorigenesis in mice. We evaluated crocetin in Swiss albino mice treated with the tobacco-specific carcinogen benzo(a)pyrene [B(a)P] for their ability to inhibit pulmonary adenoma formation and growth. Male Swiss albino mice (7 weeks old) were given 100 mg/kg B(a)P by i.p. injection, and 4 or 14 weeks later, they were given crocetin 50 mg/kg by i.p. injection 3 days/week. Crocetin (50 mg/kg body weight) reduced proliferating cells by 68% and 45% in 18 and 8 weeks of treatment respectively. The levels of glycoproteins and polyamines were significantly altered in the B(a)P-induced animals than in crocetin treatment groups. The activity of crocetin was more pronounced in the cancer. Taken together, these results indicate that crocetin was capable of inhibiting proliferation cells by inhibiting proliferating cells, glycoprotein and polyamine synthesis.
Magesh
Venkatraman
V
Department of Medical Biochemistry, Dr A.L.M.P.G. Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600113, India.
DurgaBhavani
Konga
K
Senthilnathan
Palaniyandi
P
Rajendran
Peramaiyan
P
Sakthisekaran
Dhanapal
D
D000236:Adenoma / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D001564:Benzo(a)pyrene / Q000009:adverse effects; D002338:Carotenoids / Q000494:pharmacology*; D006023:Glycoproteins / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051379:Mice; D011073:Polyamines / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D014801:Vitamin A / Q000031:analogs & derivatives
D000972:Antineoplastic Agents, Phytogenic; D006023:Glycoproteins; D006595:Hexosamines; D011073:Polyamines; D018809:Proliferating Cell Nuclear Antigen; C487773:trans-sodium crocetinate; D014801:Vitamin A; D001564:Benzo(a)pyrene; D002338:Carotenoids