Effects of Urtica dioica L. seed on lipid peroxidation, antioxidants and liver pathology in aflatoxin-induced tissue injury in rats.
This study was carried out to investigate the hepatoprotective and antioxidant properties of Urtica dioica L. seeds (UDS) extract against aflatoxin (AF)-exposure in rats. The preventive potential and antioxidant capacity of the plant's extract was evaluated by liver histopathological changes, measuring serum marker enzymes, antioxidant defense systems and lipid peroxidation (Malondialdehyde, MDA) content in some tissues of rats. Eighteen rats were randomly divided into one of three experimental groups: control, AF-treated group and AF+UDS-treated group. Rats in control group were fed with a diet without AF. Rats in AF-treated group and AF+UDS-treated group received approximately 25 microgr of AF/rat/day. AF+UDS groups also received 2 mL of UDS oils/rat/day by gavage for 90 days. Administration of UDS extract restored the AF-induced imbalance between MDA and antioxidant system towards near normal particularly in liver. Hepatoprotection by UDS is further substantiated by the almost normal histologic findings in AF+UDS-treated group as against degenerative changes in the AF-treated rats. It is concluded that UDS has a hepatoprotective effect in rats with aflatoxicosis, probably acting by promoting the antioxidative defense systems.
Yener
Zabit
Z
Department of Pathology, Yuzuncu Yil University, Faculty of Veterinary Medicine, 65080 Van, Turkey. zyener@yyu.edu.tr
Celik
Ismail
I
Ilhan
Fatma
F
Bal
Ramazan
R
D000284:Administration, Oral; D000348:Aflatoxins / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011042:Poisons / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D026982:Urtica dioica / Q000737:chemistry*
D000348:Aflatoxins; D000975:Antioxidants; D010936:Plant Extracts; D011042:Poisons; D008315:Malondialdehyde; D010088:Oxidoreductases