title: Determination of afloqualone in human plasma using liquid chromatography/tandem mass spectrometry: Application to pharmacokinetic studies in humans.
abstract: Two methods for determining the central-acting muscle relaxant afloqualone in human plasma were developed and compared using API2000 and API4000 liquid chromatography tandem mass spectrometry (LC/MS/MS) systems. In the API2000 LC/MS/MS system, afloqualone and the internal standard methaqualone were extracted from plasma using a methyl-tertiary ether. After drying the organic layer, the residue was reconstituted in a mobile phase (0.1% formic acid-acetonitrile:0.1% formic acid buffer, 80:20 v/v) and injected onto a reversed-phase C(18) column. The isocratic mobile phase was eluted at 0.2ml/min. The ion transitions monitored in multiple reaction-monitoring mode were m/z 284-->146 and 251-->117 for afloqualone and methaqualone, respectively. Sample preparation for the API4000LC/MS/MS system involved simple protein precipitation with an organic mixture (methanol:10% ZnSO(4)=8:2). The ion transitions monitored in multiple reaction-monitoring mode were m/z 284-->146 and 251-->131 for afloqualone and methaqualone, respectively. In both assays, the coefficient of variation of the precision was less than 11.8%, the accuracy exceeded 91.5%, the limit of quantification was 0.5ng/ml, and the limit of detection was 0.1ng/ml for afloqualone. Two methods were used to measure the plasma afloqualone concentration in healthy subjects after a single oral 20-mg dose of afloqualone. During subsequent application of the methods, we observed that high-concentration plasma samples (>7ng/ml) prepared using the protein precipitation method resulted in about 20% higher afloqualone concentrations than with plasma samples prepared using the liquid-liquid extraction method. We believe that this phenomenon was related to the cleanness of the sample and its chemical nature.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Hwi-Yeol
authors.initials: HY
authors.lastname: Yun
authors.forename: Seo-Pan
authors.initials: SP
authors.lastname: Lee
authors.forename: Hae Hum
authors.initials: HH
authors.lastname: Jeong
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Yoon
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Sohn
authors.forename: Sang Kyum
authors.initials: SK
authors.lastname: Kim
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Kwon