title: One-port video assisted laparoscopic kidney biopsy in standing steers.
abstract: The purpose of this study was to simplify the two-port laparoscopic renal biopsy technique used in support of pharmacokinetic studies through the application of a one-port system. Twelve Holstein steers were fasted for 24 h and sedated with acepromazine and xylaxine in preparation for laparoscopic surgery in standing stocks. Lidocaine 2% was injected to provide local anesthesia for introduction of the trocar-cannula assembly. The operating endoscope was inserted and the abdomen was insufflated with CO(2). A biopsy forceps was introduced into the channel of the operating endoscope to obtain a 100mg kidney cortex sample. Eighteen laparoscopic procedures provided 18 kidney samples suitable for pharmacokinetic studies. No complications were encountered. The one-port laparoscopic kidney biopsy is feasible and safe, and advanced skill required for triangulation is not necessary for its performance.
authors.affiliation: Center for Veterinary Medicine, Office of Research, Division of Residue Chemistry, Food and Drug Administration, 8401 Muirkirk Road, Laurel, MD 20708, USA.
authors.forename: Oscar Alberto
authors.initials: OA
authors.lastname: Chiesa
authors.forename: Jurgen
authors.initials: J
authors.lastname: von Bredow
authors.forename: Michelle
authors.initials: M
authors.lastname: Smith
authors.forename: Michael
authors.initials: M
authors.lastname: Thomas
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D007668:Kidney / Q000601:surgery*; D020706:Laparoscopes / Q000662:veterinary*; D010535:Laparoscopy / Q000379:methods / Q000662:veterinary*; D008297:Male; D014743:Videotape Recording