Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high-speed counter-current chromatography.
In order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high-speed counter-current chromatography (HSCCC). The diphase solvent system was n-hexane/EtOAc/MeOH/H(2)O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA-O-beta-D-glucopyranoside (8 mg, PAAG), and PAB-O-beta-D-glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by( 1)H and( 13)C NMR analysis or HPLC comparison with the reference compounds.
Han
Quan-Bin
QB
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, ShaTin N. T., Hong Kong, China.
Wong
Lina
L
Lai
Fanny
F
Yang
Nian-Yun
NY
Song
Jing-Zheng
JZ
Qiao
Chun-Feng
CF
Xu
Hong-Xi
HX
D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry*; D015394:Molecular Structure; D028201:Pinaceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012997:Solvents; D013997:Time Factors
D004224:Diterpenes; D005960:Glucosides; D012997:Solvents; C058391:pseudolaric acid B; C058390:pseudolaric acid A