title: [Studies on bioactive constituents of whole herbs of Vernonia cinerea].
abstract: OBJECTIVE
  To study the constituents of the whole herbs of Vernonia cinerea. by bio-activity guided isolation with PC-12 model.
  
  
  METHODS
  The constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods.
  
  
  RESULTS
  Four compounds were identified to be (+)-lirioresinol B (1), stigmasterol (2), stigmasterol-3-O-beta-D-glucoside (3), 4-sulfo-benzocyclobutene (4), and their NGF inducing activity were also investigated.
  
  
  CONCLUSIONS
  Compounds 1, 3, 4 were isolated from this genus for the first time, and compound 4 was identified as a new natural product. Compounds 1, 3, 4 showed cytotoxicity on PC-12, and compounds 2, 3, 4 showed inhibition activity. Compound 4 showed a specific effect on the survival of TrkA fibroblasts, and resulted in the inducing NGF activity.
authors.affiliation: School of life sciences, Nanjing Normal University, Nanjing 210024, China. Huaxu72@yahoo.com.cn
authors.forename: Hua-xu
authors.initials: HX
authors.lastname: Zhu
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Lin-mei
authors.initials: LM
authors.lastname: Pan
authors.forename: Zhi-da
authors.initials: ZD
authors.lastname: Min
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D016716:PC12 Cells; D011083:Polycyclic Compounds / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000737:chemistry; D035741:Vernonia / Q000737:chemistry*
chemical_list: C540437:4-sulfo-benzocyclobutene; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D011083:Polycyclic Compounds; C464587:stigmasterol glucoside; D013265:Stigmasterol