[Characterization of oxysterol binding protein homolog MgORP1 in the rice blast fungus Magnaporthe grisea].
OBJECTIVE
To study the component of ORPs (Oxysterol-binding proteins-related proteins) family in the rice fungus (Magnaporthe grisea), and the functions of MgORP1 gene with growing development of M. grisea, we constructed two MgORP1 deletion mutants and a complementation mutant.


METHODS
All proteins of ORPs have a conserved OSBP-related ligand-binding domain (ORD), which was compared by BLASTP with the sequences of M. grisea genomic DNA published in M. grisea database. One of ORP gene, designated as MgORP1, was deleted by gene replacement and then the phenotypes of delta MgORP1 were characterized.


RESULTS
Six putative ORP family proteins have been found in M. grisea, and the growth rate of hyphal colony in complete medium and conidiation of delta MgORP1 were lower, whereas the ability of conidia germination, appressoria formation and pathogenicity were normal.


CONCLUSIONS
MgORP1 gene of M. grisea was probably involved in colony growth and conidiation.
Chunhua
Lin
L
Environment and Plant Protection College, Hainan University, Danzhou 571737, China. lin3286320@126.com
Fucong
Zheng
Z
D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020082:Magnaporthe / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D017434:Protein Structure, Tertiary; D011987:Receptors, Steroid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017384:Sequence Deletion; D013172:Spores, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D014774:Virulence; D000098825:Oxysterol Binding Proteins
D005656:Fungal Proteins; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins