Stress induces tRNA cleavage by angiogenin in mammalian cells.
tRNAs play a central role in protein translation, acting as the carrier of amino acids. By cloning microRNAs, we unexpectedly obtained some tRNA fragments generated by tRNA cleavage in the anticodon loop. These tRNA fragments are present in many cell lines and different mouse tissues. In addition, various stress conditions can induce this tRNA cleavage event in mammalian cells. More importantly, angiogenin (ANG), a member of RNase A superfamily, appears to be the nuclease which cleaves tRNAs into tRNA halves in vitro and in vivo. These results imply that angiogenin plays an important physiological role in cell stress response, except for the known function of inducing angiogenesis.
Fu
Hanjiang
H
Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People's Republic of China.
Feng
Junjun
J
Liu
Qin
Q
Sun
Fang
F
Tie
Yi
Y
Zhu
Jie
J
Xing
Ruiyun
R
Sun
Zhixian
Z
Zheng
Xiaofei
X
D000818:Animals; D000926:Anticodon / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D051379:Mice; D012343:RNA, Transfer / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D013312:Stress, Physiological
D000926:Anticodon; D012343:RNA, Transfer; C046366:angiogenin; D012259:Ribonuclease, Pancreatic