title: RPPAML/RIMS: a metadata format and an information management system for reverse phase protein arrays.
abstract: BACKGROUND
  Reverse Phase Protein Arrays (RPPA) are convenient assay platforms to investigate the presence of biomarkers in tissue lysates. As with other high-throughput technologies, substantial amounts of analytical data are generated. Over 1,000 samples may be printed on a single nitrocellulose slide. Up to 100 different proteins may be assessed using immunoperoxidase or immunoflorescence techniques in order to determine relative amounts of protein expression in the samples of interest.
  
  
  RESULTS
  In this report an RPPA Information Management System (RIMS) is described and made available with open source software. In order to implement the proposed system, we propose a metadata format known as reverse phase protein array markup language (RPPAML). RPPAML would enable researchers to describe, document and disseminate RPPA data. The complexity of the data structure needed to describe the results and the graphic tools necessary to visualize them require a software deployment distributed between a client and a server application. This was achieved without sacrificing interoperability between individual deployments through the use of an open source semantic database, S3DB. This data service backbone is available to multiple client side applications that can also access other server side deployments. The RIMS platform was designed to interoperate with other data analysis and data visualization tools such as Cytoscape.
  
  
  CONCLUSIONS
  The proposed RPPAML data format hopes to standardize RPPA data. Standardization of data would result in diverse client applications being able to operate on the same set of data. Additionally, having data in a standard format would enable data dissemination and data analysis.
authors.affiliation: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. rstanisl@mdanderson.org
authors.forename: Romesh
authors.initials: R
authors.lastname: Stanislaus
authors.forename: Mark
authors.initials: M
authors.lastname: Carey
authors.forename: Helena F
authors.initials: HF
authors.lastname: Deus
authors.forename: Kevin
authors.initials: K
authors.lastname: Coombes
authors.forename: Bryan T
authors.initials: BT
authors.lastname: Hennessy
authors.forename: Gordon B
authors.initials: GB
authors.lastname: Mills
authors.forename: Jonas S
authors.initials: JS
authors.lastname: Almeida
mesh_terms: D000465:Algorithms; D003628:Database Management Systems; D030562:Databases, Protein; D011381:Programming Languages; D040081:Protein Array Analysis / Q000379:methods*; D012984:Software