Characterization of an NADH oxidase of the flavin-dependent disulfide reductase family from Methanocaldococcus jannaschii.
Methanocaldococcus jannaschii, a deeply rooted hyperthermophilic anaerobic methanarchaeon from a deep-sea hydrothermal vent, carries an NADH oxidase (Nox) homologue (MJ0649). According to the characteristics described here, MJ0649 represents an unusual member within group 3 of the flavin-dependent disulfide reductase (FDR) family. This FDR group comprises Nox, NADH peroxidases (Npx) and coenzyme A disulfide reductases (CoADRs); each carries a Cys residue that forms Cys-sulfenic acid during catalysis. A sequence analysis identified MJ0649 as a CoADR homologue. However, recombinant MJ0649 (rMJNox), expressed in Escherichia coli and purified to homogeneity an 86 kDa homodimer with 0.27 mol FAD (mol subunit)(-1), showed Nox but not CoADR activity. Incubation with FAD increased FAD content to 1 mol (mol subunit)(-1) and improved NADH oxidase activity 3.4-fold. The FAD-incubated enzyme was characterized further. The optimum pH and temperature were > or =10 and > or =95 degrees C, respectively. At pH 7 and 83 degrees C, apparent Km values for NADH and O2 were 3 microM and 1.9 mM, respectively, and the specific activity at 1.4 mM O2 was 60 micromol min(-1) mg(-1); 62 % of NADH-derived reducing equivalents were recovered as H2O2 and the rest probably generated H2O. rMjNox had poor NADPH oxidase, NADH peroxidase and superoxide formation activities. It reduced ferricyanide, plumbagin and 5,5'-dithiobis(2-nitrobenzoic acid), but not disulfide coenzyme A and disulfide coenzyme M. Due to a high Km, O2 is not a physiologically relevant substrate for MJ0649; its true substrate remains unknown.
Case
Christopher L
CL
Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Rodriguez
Jason R
JR
Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.|Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Mukhopadhyay
Biswarup
B
Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.|Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.|Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
D000595:Amino Acid Sequence; D019641:DNA, Archaeal / Q000032:analysis; D019605:Euryarchaeota / Q000201:enzymology* / Q000235:genetics; D005415:Flavins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013696:Temperature
D019641:DNA, Archaeal; D005415:Flavins; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; C431348:protein disulfide oxidoreductase, Methanococcus jannaschii