title: Acasiane A and B and farnesirane A and B, diterpene derivatives from the roots of Acacia farnesiana.
abstract: Four new diterpenes, acasiane A ( 1), acasiane B ( 2), farnesirane A ( 3), and farnesirane B ( 4), along with three known diterpenes ( 5 - 7), two triterpenes ( 8 and 9), and eight flavonoids ( 10 - 17) were isolated from the roots of Acacia farnesiana. The structures and relative configurations of these compounds were determined by various spectroscopic and x-ray analyses. All isolated compounds were evaluated for their in vitro cytotoxic activities against six human cancer cell lines (Hep G2, Hep 3B, MDA-MB-231, MCF-7, A549, and Ca9 - 22) with the MTT method. Betulinic acid ( 8) displayed moderate cytotoxicity (1.70 - 5.74 microg/mL) towards five human cancer cell lines and the flavonoids had slight effects. In addition, 8, diosmetin ( 13), and 3',4',5-trihydroxy-7-methoxyflavone ( 15) slightly inhibited superoxide anion generation or elastase release by human neutrophils, indicating moderate anti-inflammatory activities.
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: An-Shen
authors.initials: AS
authors.lastname: Lin
authors.forename: Chia-Rong
authors.initials: CR
authors.lastname: Lin
authors.forename: Ying-Chi
authors.initials: YC
authors.lastname: Du
authors.forename: Tilo
authors.initials: T
authors.lastname: Lübken
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Chiang
authors.forename: I-Hsiao
authors.initials: IH
authors.lastname: Chen
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Chen
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Yen
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots; D013481:Superoxides / Q000037:antagonists & inhibitors; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D000094062:Betulinic Acid
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D005419:Flavonoids; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D014315:Triterpenes; D013481:Superoxides; D019272:Leukocyte Elastase; D000094062:Betulinic Acid