Pterostilbene from Vitis coignetiae protect H2O2-induced inhibition of gap junctional intercellular communication in rat liver cell line.
In this study, we identified various stilbenoids derived from Vitis coignetiae and investigated the protective effect of the main component, pterostilbene, against the inhibition of gap junctional intercellular communication (GJIC) induced by H(2)O(2) in WB-F344 rat liver epithelial cells. We analyzed seven kinds of stilbenoids, pterostilbene, astringin, piceid, vitisin, rhaponticin, resveratrol, and rhapontigenin, using DAD/UV HPLC. Total stilbenoid content was 127.37+/-19.29 mg/100g dry weight. Pretreatment with 0.5, 1.0, and 5.0 microM pterostilbene for 24h was shown to recover GJIC blocked by 500 microM H(2)O(2). Pretreatment with pterostilbene prevented the inhibition of GJIC via the down-regulation of connexin43 phosphorylation by the inactivation of ERK1/2 and p38 MAP kinase. Our results suggest that pterostilbene may be a functional chemopreventative agent and that dietary exposure of pterostilbene would be helpful for improving health.
Kim
Ji-Sun
JS
Food Function Research Center, Food Convergence Technology Division, Korea Food Research Institute, Seongnam, Baekhyun-Dong, Bundang-Ku, Sungnam-Si, Gyeonggi-Do 463-746, Republic of Korea. jskim320@hotmail.com
Ha
Tae-Youl
TY
Ahn
Jiyun
J
Kim
Hyun-Ku
HK
Kim
Suna
S
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D018031:Connexin 43 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D017629:Gap Junctions / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity; D048748:MAP Kinase Kinase Kinase 3 / Q000037:antagonists & inhibitors; D016877:Oxidants / Q000633:toxicity; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors
D000975:Antioxidants; D018031:Connexin 43; D016877:Oxidants; D010936:Plant Extracts; D013267:Stilbenes; C107773:pterostilbene; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; D048748:MAP Kinase Kinase Kinase 3