Goat serum as an alternative to establish cell culture from Indian major carp, Cirrhinus mrigala.
Serum from goat, calf, and chicken sources were evaluated in terms of attachment, growth, and proliferation of explants of Indian major carp, Cirrhinus mrigala. The attachment of explants viz. heart, liver, and kidney was directly proportional to the concentration of the serum. Among these sera, the highest percentage of attachment, growth, and proliferation was recorded for 10% goat serum and 15% newborn calf serum without affecting their cell morphology. On contrary to these sera, chicken serum at 15% concentration was found to be mildly toxic for all the explants. The cell count was significantly high for the kidney, liver, and heart at 10% goat serum among all the tested sera as well as concentration. Similarly, the liver, heart, and kidney explants were found to survive up to the tenth, seventh, and ninth passage, respectively. Therefore, the goat serum at 10% concentration can be used as effectively as newborn calf serum for routine culture of fish cells.
Nanda
P K
PK
Central Institute of Freshwater Aquaculture, Bhubaneswar, India.
Swain
P
P
Nayak
S K
SK
Dash
S
S
Routray
P
P
Swain
S K
SK
Patra
B C
BC
D000818:Animals; D002347:Carps; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006041:Goats; D007194:India; D008099:Liver / Q000166:cytology; D009928:Organ Specificity; D044967:Serum