title: [Protective effect of ulinastatin on myocardium after cardiopulmonary resuscitation in rats].
abstract: OBJECTIVE
  To investigate the changes in serum creatine kinase isoenzyme (CK-MB) and cardiac troponin I (cTnI) levels and the ultrastructure of myocardium, and the effect of ulinastatin after cardiopulmonary resuscitation (CPR) in rats.
  
  
  METHODS
  One hundred and twenty Sprague-Dawley (SD) rats were randomly divided into sham operation group, CPR group and ulinastatin group. And each group was divided into five subgroups (n=8) according to time points of 0.5, 3, 6, 12, 24 hours after tracheostomy in sham operation group or after restoration of spontaneous circulation (ROSC) in CPR group and ulinastatin group. Cardiac arrest (CA) and CPR models were reproduced by asphyxia in CPR group and ulinastatin group, with injection of ulinastatin 100 kU/kg via carotid 2 minutes after ROSC in ulinastatin group. Normal saline was given in the same dosage as ulinastatin in CPR group. Serum samples was taken at each time point of subgroups for measuring serum levels of CK-MB and cTnI. The ultrastructure changes in myocardium were observed under the electron microscope.
  
  
  RESULTS
  The serum levels of CK-MB and cTnI were increased from 0.5 hours after ROSC in CPR group and ulinastatin group compared with sham operation group ( all P<0.01), and CK-MB peaked at 12 hours, then decreased. The serum CK-MB or cTnI levels started to decreased from 3 hours or 0.5 hours after ROSC in ulinastatin group compared with CPR group (P<0.05 or P<0.01). There were milder pathological changes of ultrastructure of myocardium in ulinastatin group than in CPR group.
  
  
  CONCLUSIONS
  Ulinastatin could decrease serum levels of CK-MB and cTnI, and alleviate myocardial injury after CPR in rats.
authors.affiliation: Department of Emergency, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, Zhejiang, China.
authors.forename: Shou-Quan
authors.initials: SQ
authors.lastname: Chen
authors.forename: Shi-Fang
authors.initials: SF
authors.lastname: Zhou
authors.forename: Zhang-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Yan
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Huang
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Cheng
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Li
authors.forename: Wan-Tie
authors.initials: WT
authors.lastname: Wang
mesh_terms: D000818:Animals; D016887:Cardiopulmonary Resuscitation; D052279:Creatine Kinase, MB Form / Q000097:blood; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000494:pharmacology*; D006323:Heart Arrest / Q000097:blood / Q000188:drug therapy / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000648:ultrastructure*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019210:Troponin I / Q000097:blood
chemical_list: D006023:Glycoproteins; D019210:Troponin I; D052279:Creatine Kinase, MB Form; C028665:urinastatin