Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: implication in the migration.
Umbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC- and P-MSC possess immunophenotypic and functional characteristics similar to BM-MSC. However, their migration capacity, which is indispensable during tissue regeneration process, is unclear. Under defined conditions, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-folds higher than that of UC-MSC, respectively. By the use of 2-DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Consistent with their migration capacity, the levels of migration enhancing proteins including cathepsin B, cathepsin D and prohibitin,were significantly lower in UC-MSC when compared with those in BM- and P-MSC. For the migration inhibiting proteins such as plasminogen activator inhibitor-1 (PAI-1) and manganese superoxide dismutase, higher expression was found in the UC-MSC. We also showed that the overexpression of the PAI-1 impaired the migration capacity of BM- and P-MSC while silencing of PAI-1 enhanced the migration capacity of UC-MSC. Our study indicates that PAI-1 and other migration-related proteins are pivotal in governing the migration capacity of MSC.
Li
Guo
G
Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, PR China.
Zhang
Xiao-ai
XA
Wang
Hua
H
Wang
Xin
X
Meng
Chun-ling
CL
Chan
Chu-yan
CY
Yew
David Tai Wai
DT
Tsang
Kam Sze
KS
Li
Karen
K
Tsai
Sau-na
SN
Ngai
Sai-ming
SM
Han
Zhong Chao
ZC
Lin
Marie Chia-mi
MC
He
Ming-liang
ML
Kung
Hsiang-fu
HF
D001854:Bone Marrow Cells / Q000737:chemistry*; D002401:Cathepsin B / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D010920:Placenta / Q000737:chemistry* / Q000166:cytology; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D014470:Umbilical Cord / Q000737:chemistry* / Q000166:cytology
D017395:Plasminogen Activator Inhibitor 1; D020543:Proteome; C051321:SERPINE1 protein, human; D002401:Cathepsin B