Protective effect of tripeptide in the presence of cyclophosphamide on the growth of cultured lymphoid tissue from rats of different age.
We studied the effect of tripeptide T-38 (Lys-Glu-Asp) in the presence of cyclophosphamide on cell proliferation and apoptosis in explants of splenic lymphoid tissue from young and old rats. Peptide T-38 in a concentration of 0.05 ng/ml produced a stimulatory effect on the growth zone of the explants. Addition of 1 mg/ml cyclophosphamide to the culture medium suppressed cell proliferation, which was associated with enhanced expression of proapoptotic p53 protein. Under conditions of combined treatment with cyclophosphamide and T-38 no inhibiting effect of the cytostatics was observed. Thus, tripeptide T-38 in the presence of cytostatics produces a protective effect on cell proliferation in lymphoid tissue explants.
Chalisova
N I
NI
St. Petersburg Institute of Bioregulation and Gerontology, Northwestern Division of the Russian Academy of Medical Sciences, Russia. ni_chalisova@mail.ru
Lesnyak
V V
VV
Oganezova
E V
EV
Rizhak
G A
GA
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D046509:Tissue Culture Techniques; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D009842:Oligopeptides; D016159:Tumor Suppressor Protein p53; C533570:lysyl-glutamyl-aspartic acid; D003520:Cyclophosphamide