title: In obese and non-obese adults, the cis-regulatory rs361072 promoter variant of PIK3CB is associated with insulin resistance not with type 2 diabetes.
abstract: We recently reported that rs361072, a promoter C/T variant of p110beta, the catalytic subunit of PI3-kinase, was associated with a protection from insulin resistance (IR) in Caucasian adolescents in proportion of their body mass. We tested if this cis-regulatory QTL is associated with IR and type 2 diabetes in 7885 middle-aged obese and non-obese adults of European ancestry. We genotyped rs361072 in 1139 non-diabetic obese (NDO) European adults, in whom IR was estimated by the HOMA-IR index. We also studied 427 type 2 diabetic obese adults (DO) and 424 diabetic non-obese (DNO) adults to test whether their disease status was associated with a decreased prevalence of the protective variant. The prevalence of rs361072 and association with IR was also examined in 5895 non-obese non-diabetic adults (NDNO). rs361072 was associated with HOMA-IR (p=4.10(-4)) in NDO, so that C/C patients had a 17% decrease of this index (p=0.002). A statistical trend (p=1.1.10(-2)) for the same genotypic differences was also observed in NDNO adults, but of insignificant magnitude (4.2%). The distribution of rs361072 genotype was comparable in NDO, DO, DNO and NDNO individuals. Allele C of rs361072 is associated with a protection from IR in obese and non-obese adults, but has no significant effect, however, on diabetes risk in obese or non-obese Europeans.
authors.affiliation: Endocrinology and Nutrition Department, Pitié-Salpétrière Hospital, Paris, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Clément
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Stunff
authors.forename: Aline
authors.initials: A
authors.lastname: Meirhaeghe
authors.forename: Agnès
authors.initials: A
authors.lastname: Dechartres
authors.forename: Jean
authors.initials: J
authors.lastname: Ferrieres
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Christian
authors.initials: C
authors.lastname: Boitard
authors.forename: Philippe
authors.initials: P
authors.lastname: Amouyel
authors.forename: Pierre
authors.initials: P
authors.lastname: Bougnères
mesh_terms: D000328:Adult; D058534:Class I Phosphatidylinositol 3-Kinases; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D044465:White People / Q000235:genetics
chemical_list: D058534:Class I Phosphatidylinositol 3-Kinases; D019869:Phosphatidylinositol 3-Kinases; C497132:PIK3CB protein, human