title: Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: implication in the migration.
abstract: Umbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC- and P-MSC possess immunophenotypic and functional characteristics similar to BM-MSC. However, their migration capacity, which is indispensable during tissue regeneration process, is unclear. Under defined conditions, the migration capacity of BM- and P-MSC was found 5.9- and 3.2-folds higher than that of UC-MSC, respectively. By the use of 2-DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Consistent with their migration capacity, the levels of migration enhancing proteins including cathepsin B, cathepsin D and prohibitin,were significantly lower in UC-MSC when compared with those in BM- and P-MSC. For the migration inhibiting proteins such as plasminogen activator inhibitor-1 (PAI-1) and manganese superoxide dismutase, higher expression was found in the UC-MSC. We also showed that the overexpression of the PAI-1 impaired the migration capacity of BM- and P-MSC while silencing of PAI-1 enhanced the migration capacity of UC-MSC. Our study indicates that PAI-1 and other migration-related proteins are pivotal in governing the migration capacity of MSC.
authors.affiliation: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, PR China.
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Xiao-ai
authors.initials: XA
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Meng
authors.forename: Chu-yan
authors.initials: CY
authors.lastname: Chan
authors.forename: David Tai Wai
authors.initials: DT
authors.lastname: Yew
authors.forename: Kam Sze
authors.initials: KS
authors.lastname: Tsang
authors.forename: Karen
authors.initials: K
authors.lastname: Li
authors.forename: Sau-na
authors.initials: SN
authors.lastname: Tsai
authors.forename: Sai-ming
authors.initials: SM
authors.lastname: Ngai
authors.forename: Zhong Chao
authors.initials: ZC
authors.lastname: Han
authors.forename: Marie Chia-mi
authors.initials: MC
authors.lastname: Lin
authors.forename: Ming-liang
authors.initials: ML
authors.lastname: He
authors.forename: Hsiang-fu
authors.initials: HF
authors.lastname: Kung
mesh_terms: D001854:Bone Marrow Cells / Q000737:chemistry*; D002401:Cathepsin B / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D010920:Placenta / Q000737:chemistry* / Q000166:cytology; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D014470:Umbilical Cord / Q000737:chemistry* / Q000166:cytology
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D020543:Proteome; C051321:SERPINE1 protein, human; D002401:Cathepsin B