A novel model for external biliary drainage in rat orthotopic liver transplantation.
OBJECTIVE
The objective of this study was to establish a stable model of orthotopic liver transplantation with external biliary drainage in rats, to provide the possibility for dynamic detection of bile characteristics after transplantation.


METHODS
Ninety-eight Sprague Dawley rats were divided randomly into a control group (n = 30) and an external drainage group (n = 68). Biliary extra-drainage group rats were established as follows: an epidural catheter was implanted into the common bile duct and an appropriate hose was connected from the outside. A stoma was made into the high jejunum, and a hose was implanted. Then the 2 hoses were induced to connect with each other in the subcutis of the right lateral abdominal wall.


RESULTS
The operative success rate and 1-week and 2-week survival rates of the extra-drainage group were 94.1% (64/68), 88.2% (60/68), and 79.4% (54/68), respectively. Meanwhile, in the control group, the operative success rate and 1-week and 2-week survival rates were 93.3% (28/30), 90% (27/30), and 83.3% (25/30), respectively. There were no significant differences at any time between the 2 groups (p > 0.05).


CONCLUSIONS
The biliary extra-drainage model in rat orthotopic liver transplantation provides a novel, simple, and reliable method for dynamic collection of bile. The operation success rate was satisfactory. This model was successful and could be applied in various experimental studies.
Hang
H-L
HL
Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, Nanjing, Jiangsu Province, PR China.
Qiu
Y-D
YD
Zhu
X-H
XH
Mao
L
L
Ding
Y-T
YT
D000818:Animals; D001646:Bile; D003135:Common Bile Duct / Q000601:surgery; D004322:Drainage / Q000379:methods*; D005240:Feasibility Studies; D007582:Jejunostomy; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley