title: A scFv antibody-based immunoaffinity chromatography column for clean-up of bisphenol A-contaminated water samples.
abstract: This is a report on the development of immunoaffinity chromatography using a column of silica gel with an immobilized single-chain variable fragment (scFv) antibody specific to bisphenol A (BPA) for cleanup of BPA-contaminated water samples. The BBA-2187 scFv antibody specific to BPA was purified from the periplasmic fractions of the recombinant Escherichia coli. After a sample of BPA-contaminated river water was applied to the immunoaffinity column, the background signal intensity observed in high-performance liquid chromatography (HPLC) analysis of the eluates was markedly lower than that observed in HPLC analysis of the eluates from an Oasis HLB cartridge treated with the same sample. The immunoaffinity column efficiently concentrated BPA from actual river water samples with different matrices. Our results demonstrate that the immunoaffinity column with immobilized BBA-2187 scFv antibody is efficient for the cleanup of BPA-contaminated water samples from different sources.
authors.affiliation: Research Center for Environmental Genomics and Graduate School of Science and Technology, Kobe University, Rokkodaicho, Nada-ku, Kobe, Hyogo, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Inui
authors.forename: Akira
authors.initials: A
authors.lastname: Takehara
authors.forename: Fumito
authors.initials: F
authors.lastname: Doi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nishi
authors.forename: Mikio
authors.initials: M
authors.lastname: Takai
authors.forename: Shiro
authors.initials: S
authors.lastname: Miyake
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohkawa
mesh_terms: D055910:Antibodies, Immobilized / Q000032:analysis / Q000276:immunology; D001559:Benzhydryl Compounds; D002846:Chromatography, Affinity / Q000379:methods*; D007135:Immunoglobulin Variable Region / Q000032:analysis / Q000276:immunology; D007158:Immunologic Techniques; D010636:Phenols / Q000032:analysis* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology; D018508:Water Purification / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D055910:Antibodies, Immobilized; D001559:Benzhydryl Compounds; D007135:Immunoglobulin Variable Region; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds