title: Rotenone damages striatal organotypic slice culture.
abstract: Epidemiological studies suggest the involvement of pesticides in the etiology of Parkinson's disease. Exposure to rotenone results in degeneration of the nigrostriatal pathway through inhibition of complex I. Organotypic striatal slice cultures were prepared from brains of adult mice and treated with rotenone (0.01, 0.05, 0.1, and 1 mM) for 48 h. Lactate dehydrogenase activity was elevated by 167% at 1 mM of rotenone. Using fluorescent indicators, membrane damage was up to 130% as measured by propidium iodide fluorescence, and superoxide (DHE) and nitric oxide (DAF-FM) formation were increased by 195% and 774% at 1 mM of rotenone, respectively, compared to controls. The study concludes that formation of radicals mediated striatal degeneration by rotenone.
authors.affiliation: Institute of Medical Chemistry, Veterinary Medical University, Vienna, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Moldzio
authors.forename: Christina
authors.initials: C
authors.lastname: Piskernik
authors.forename: Khaled
authors.initials: K
authors.lastname: Radad
authors.forename: Wolf-Dieter
authors.initials: WD
authors.lastname: Rausch
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D007202:Indicators and Reagents / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010300:Parkinson Disease / Q000473:pathology; D011419:Propidium / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D014475:Uncoupling Agents / Q000494:pharmacology*
chemical_list: D007202:Indicators and Reagents; D007306:Insecticides; D017382:Reactive Oxygen Species; D014475:Uncoupling Agents; D012402:Rotenone; D009569:Nitric Oxide; D011419:Propidium; D007770:L-Lactate Dehydrogenase