title: The efficacy of ethanolic extract of Lemon verbena on the skin infection due to Staphylococcus aureus in an animal model.
abstract: Daily increasing of Staphylococcus aureus resistance to various antibiotics in particular penicillin and Methecilin has led the scientist to look fore new medicines in this area. In an in vitro laboratory studies, it has been demonstrated that ethanolic extract of Lemon verbena can prevent the growth of Staphylococcus aureus. In this study the efficacy of ethanolic extract of Lemon verbena against Staphylococcus aureus skin infection were assessed in an in vivo, in animal model. 200lambda of Staphylococcus aureus suspension, were inoculated intradermally on the shoulder of 63 laboratory 20-30 g mice. the mice were divided in to 4 groups, 2 control groups: Negative (without treatment) and positive (treated with Mupirucin) and 2 test groups that treated for 7 days by ointment prepared from ethanolic extract of Lemon verbena (group 3), or injection of Lemon verbena solution (group 4). The status of wounds, the rate of recovery was studied and the presence of local pus after dissection of mice on day 8 recorded and compared with each other. The wound appearance in the second day, on the injection site of S. aureus, in Group 1, 4, 3 and 2 were 84.2, 66.7, 46.2 and 23.1%, respectively. In the final day, the lesion still was remained in 78.9, 23.1, 92.3 and 77.7% in groups 1 -4, respectively. The necrotic and wide wounds were more observed in groups 1 and 3 vs two other groups. The results from this investigation indicate that the ointment prepared from ethanolic extract of Lemon verbena is a proper medication to prevent the skin infection by Staphylococcus aureus in early phase.
authors.affiliation: Golestan University of Medical Sciences, Iran.
authors.forename: Ezzat Ollah
authors.initials: EO
authors.lastname: Ghaemi
authors.forename: Didar
authors.initials: D
authors.lastname: Khorshidi
authors.forename: Abdolvahab
authors.initials: A
authors.lastname: Moradi
authors.forename: Akhter
authors.initials: A
authors.lastname: Seifi
authors.forename: Masomeh
authors.initials: M
authors.lastname: Mazendrani
authors.forename: Masod
authors.initials: M
authors.lastname: Bazouri
authors.forename: Azad Reza
authors.initials: AR
authors.lastname: Mansourian
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D013207:Staphylococcal Skin Infections / Q000188:drug therapy* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000378:metabolism*; D016896:Treatment Outcome; D032412:Verbena / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects; D014946:Wound Infection / Q000188:drug therapy
chemical_list: D000891:Anti-Infective Agents, Local; D010936:Plant Extracts; D000431:Ethanol