title: CMY-31 and CMY-36 cephalosporinases encoded by ColE1-like plasmids.
abstract: Two CMY-2 derivatives, CMY-31 (Gln(215)-->Arg) from Salmonella enterica serotype Newport and CMY-36 (Ala(77)-->Cys and Gln(193)-->Glu) from Klebsiella pneumoniae, were characterized. Both cephalosporinases functionally resembled CMY-2. bla(CMY) alleles occurred as parts of a putative transposon comprising ISEcp1B and a Citrobacter freundii-derived sequence carried by ColE1-like plasmids similar to CMY-5-encoding pTKH11 from Klebsiella oxytoca.
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Zioga
authors.forename: J M
authors.initials: JM
authors.lastname: Whichard
authors.forename: S D
authors.initials: SD
authors.lastname: Kotsakis
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001120:Arginine / Q000378:metabolism; D001483:Base Sequence; D002510:Cephalosporinase / Q000235:genetics*; D016971:Citrobacter freundii / Q000201:enzymology; D003545:Cysteine / Q000378:metabolism; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D007526:Isoelectric Point; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013379:Substrate Specificity; D014170:Transformation, Genetic; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D065093:beta-Lactamase Inhibitors; D005973:Glutamine; D001120:Arginine; D002510:Cephalosporinase; D001618:beta-Lactamases; D003545:Cysteine