Localization of Entamoeba histolytica amebopore in amebic liver abscesses in hamsters.
Amebopore was purified from axenically grown trophozoites of the Entamoeba histolytica strain HM1:IMSS. The purification procedure involved Mono Q anion-exchange chromatography and electroelution. Sequence analysis of the final product revealed that amebopore A was completely pure. Polyclonal antibodies against the purified amebopore were obtained from rabbits, and Western blot studies demonstrated their specificity. Sections of experimental, acute (1, 2, 3, and 4 days), amebic liver abscesses produced in hamsters were stained with the anti-amebopore antibody; in all the analyzed stages, amebopore appeared as a constitutively expressed cytoplasmic molecule in trophozoites. No extracellular or hepatocyte-membrane amebopore was found. This study is the first to trace amebopore in an in vivo model of amebic liver abscesses.
González
A
A
Department of Experimental Medicine, Faculty of Medicine, National Autonomous University of Mexico and General Hospital, Mexico City, Mexico. metaugus@yahoo.com
Monterrubio
D
D
Nequiz
M
M
López
R
R
Olivos
A
A
García de Léon
C
C
Tello
E
E
Salaiza
N
N
Ramos
E
E
Gudiño
M
M
Montfort
I
I
Pérez-Tamayo
R
R
D000818:Animals; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D007150:Immunohistochemistry; D008101:Liver Abscess, Amebic / Q000469:parasitology*