title: Sox9-dependent expression of Gstm6 in Sertoli cells during testis development in mice.
abstract: Glutathione S-transferases (GSTs) are an important family of multifunctional enzymes that play a role in the protection of tissues by the detoxification of hazardous and carcinogenic compounds. We found previously that Gstm6 is upregulated in the somatic cells of male mouse fetal gonads relative to female gonads. In this study, we describe the spatial and temporal expression pattern of Gstm6 during mouse development. We show that Gstm6 is predominantly expressed in the reproductive system, at significantly higher levels in XY gonads compared with XX gonads from 11.5 dpc onwards, and remains expressed in the testes in adult mice. Its expression is associated with the Sertoli cell lineage, and is dependent on the expression of the male sex-determining gene Sox9. Our data suggest that Gstm6 plays a male-specific role in gonad development or function, possibly by modulating the exposure of somatic tissue and/or germ cells to endogenous or exogenous toxicants.
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Annemiek
authors.initials: A
authors.lastname: Beverdam
authors.forename: Terje
authors.initials: T
authors.lastname: Svingen
authors.forename: Stefan
authors.initials: S
authors.lastname: Bagheri-Fam
authors.forename: Pascal
authors.initials: P
authors.lastname: Bernard
authors.forename: Peter
authors.initials: P
authors.lastname: McClive
authors.forename: Mathew
authors.initials: M
authors.lastname: Robson
authors.forename: Mahdi Banan
authors.initials: MB
authors.lastname: Khojasteh
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Salehi
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Sinclair
authors.forename: Vincent R
authors.initials: VR
authors.lastname: Harley
authors.forename: Peter
authors.initials: P
authors.lastname: Koopman
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005982:Glutathione Transferase / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010053:Ovary / Q000196:embryology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000196:embryology*
chemical_list: D005982:Glutathione Transferase; D055758:SOX9 Transcription Factor; C527933:Sox9 protein, mouse; C412602:glutathione S-transferase Mu 2