title: Functions of Fyn kinase in the completion of meiosis in mouse oocytes.
abstract: Oocyte maturation invokes complex signaling pathways to achieve cytoplasmic and nuclear competencies for fertilization and development. The Src-family kinases FYN, YES and SRC are expressed in mammalian oocytes but their function during oocyte maturation remains an open question. Using chemical inhibitor, siRNA knockdown, and gene deletion strategies the function of Src-family kinases was evaluated in mouse oocytes during maturation under in vivo and in vitro conditions. Suppression of Src-family as a group with SKI606 greatly reduced meiotic cell cycle progression to metaphase-II. Knockdown of FYN kinase expression after injection of FYN siRNA resulted in an approximately 50% reduction in progression to metaphase-II similar to what was observed in oocytes isolated from FYN (-/-) mice matured in vitro. Meiotic cell cycle impairment due to a Fyn kinase deficiency was also evident during oocyte maturation in vivo since ovulated cumulus oocyte complexes collected from FYN (-/-) mice included immature metaphase-I oocytes (18%). Commonalities in meiotic spindle and chromosome alignment defects under these experimental conditions demonstrate a significant role for Fyn kinase activity in meiotic maturation.
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Blvd, mail stop 3043, Kansas City, KS 66160, USA. Lmcginnis2@kumc.edu
authors.forename: Lynda K
authors.initials: LK
authors.lastname: McGinnis
authors.forename: William H
authors.initials: WH
authors.lastname: Kinsey
authors.forename: David F
authors.initials: DF
authors.lastname: Albertini
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009865:Oocytes / Q000166:cytology / Q000201:enzymology* / Q000502:physiology*; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000378:metabolism; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D047428:Protein Kinase Inhibitors; D034741:RNA, Small Interfering; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn