New theophylline-activated Diels-Alderase ribozymes by molecular engineering.
Allosteric ribozymes were developed by rational design and step-wise optimization. These ribozymes accelerate a Diels-Alder reaction between anthracene and maleimide derivatives. The optimized sequence is efficiently activated by theophylline under single and multiple turnover conditions and distinguishes between structurally similar effector molecules.
Petermeier
Markus
M
Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120, Heidelberg, Germany.
Jäschke
Andres
A
D001483:Base Sequence; D002384:Catalysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry*; D013806:Theophylline / Q000737:chemistry*
D016337:RNA, Catalytic; D013806:Theophylline