[Study of the interaction of atrazine and ct-DNA by fluorescence probe technique].
The interaction atrazine and ct-DNA was studied using fluorescence probe and UV absorption spectroscopy technique. Ethidium bromide (EB) was used as a probe. The effects of atrazine, phosphate, concentration of ions, and potassium iodide on the fluorescence of ct-DNA-EB system were investigated. The results showed that the fluorescence quenching process of atrazine to ct-DNA-EB system was proved to be the simultaneous dynamic and static quenching model. The phenomena of fluorescence quenching of ct-DNA-atrazine system by KI and hyperchromicity and the red shift of DNA UV-absorption caused by atrazine indicated the existence of intercalation between atrazine and ct-DNA. The experiments of phosphate and ionic strength showed that atrazine could combine with the phosphate group of ct-DNA by non-characteristic static force, and this action could be inhibited by high ionic strength.
Ma
Jing
J
College of Science, China Agricultural University, Beijing 100193, China majing84113@163.com
Meng
Qing-xiang
QX
Ren
Li-ping
LP
Zhang
Tao
T
Mu
Hao-jie
HJ
D000818:Animals; D001280:Atrazine / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry*; D008956:Models, Chemical; D011193:Potassium Iodide / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011193:Potassium Iodide; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; D001280:Atrazine