title: The role of haemoglobin A(2) testing in the diagnosis of thalassaemias and related haemoglobinopathies.
abstract: The increase in haemoglobin (Hb)A(2) level is the most significant parameter in the identification of beta thalassaemia carriers. However, in some cases the level of HbA(2) is not typically elevated and some difficulties may arise in making the diagnosis. For these reasons the quantification of HbA(2) has to be performed with great accuracy and the results must be interpreted together with other haematological and biochemical evidence. The present document includes comments on the need for accuracy and standardisation, and on the interpretation of the HbA(2) value, reviewing the most crucial aspects related to this test. A practical flow-chart is presented to summarise the significance of HbA(2) estimation in different thalassaemia syndromes and related haemoglobinopathies.
authors.affiliation: Center for Metrological Traceability in Laboratory Medicine (CIRME) and Department of Science and Biomedical Technology, University of Milan, Milan, Italy. andrea.mosca@unimi.it
authors.forename: A
authors.initials: A
authors.lastname: Mosca
authors.forename: R
authors.initials: R
authors.lastname: Paleari
authors.forename: G
authors.initials: G
authors.lastname: Ivaldi
authors.forename: R
authors.initials: R
authors.lastname: Galanello
authors.forename: P C
authors.initials: PC
authors.lastname: Giordano
mesh_terms: D000465:Algorithms; D001800:Blood Specimen Collection / Q000379:methods; D006580:Genetic Carrier Screening / Q000379:methods; D006443:Hemoglobin A2 / Q000032:analysis*; D006453:Hemoglobinopathies / Q000175:diagnosis; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D013789:Thalassemia / Q000097:blood / Q000175:diagnosis*
chemical_list: D006443:Hemoglobin A2