ROS scavenging capacity and neuroprotective effect of alpha-mangostin against 3-nitropropionic acid in cerebellar granule neurons.
Alpha-mangostin is a xanthone with antioxidant properties isolated from mangosteen fruit. The reactive oxygen species (ROS) scavenging capacity and the potential protective effect of alpha-mangostin against the mitochondrial toxin 3-nitropropionic acid (3-NP) in primary cultures of cerebellar granule neurons (CGNs) were studied in the present work. It was found that alpha-mangostin was able to scavenge in a concentration-dependent way singlet oxygen, superoxide anion and peroxynitrite anion. In contrast, alpha-mangostin was unable to scavenge hydroxyl radicals and hydrogen peroxide. Furthermore, alpha-mangostin was able to ameliorate in a concentration-dependent way the neuronal death induced by 3-NP. This protective effect was associated with an amelioration of 3-NP-induced reactive oxygen species formation. It is concluded that alpha-mangostin is able to scavenge directly several ROS and has a neuroprotective effect against 3-NP in primary cultures of CGNs, which is associated with its ability to ameliorate 3-NP-induced ROS production.
Pedraza-Chaverrí
José
J
Departamento de Biología, Facultad de Química, Laboratorio 209, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, México. pedraza@unam.mx
Reyes-Fermín
Laura María
LM
Nolasco-Amaya
Eva Guadalupe
EG
Orozco-Ibarra
Marisol
M
Medina-Campos
Omar Noel
ON
González-Cuahutencos
Octavio
O
Rivero-Cruz
Isabel
I
Mata
Rachel
R
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009574:Nitro Compounds / Q000633:toxicity; D011422:Propionates / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D044004:Xanthones / Q000494:pharmacology*
D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D009574:Nitro Compounds; D011422:Propionates; D017382:Reactive Oxygen Species; D044004:Xanthones; C015392:3-nitropropionic acid; C021053:mangostin