A suicidal DNA vaccine co-expressing two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus antigens induce protective responses.
We constructed a suicidal DNA vaccine pSFV-ORF5m/ORF6 co-expressing GP5m (a modified GP5) and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV). In mice immunization, specific immune responses were elicited by the suicidal DNA vaccine pSFV-ORF5m/ORF6. The immunogenicity and protective efficiency was then evaluated in piglets immunized with pSFV-ORF5m/ORF6 before virus challenge: PRRSV-specific neutralizing antibodies and lymphocyte proliferative responses were developed. Post-PRRSV challenge, these immune responses were further boosted and partial protection was obtained.
Jiang
Yunbo
Y
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shi-zi-shan Street, 430070 Wuhan, Hubei, People's Republic of China.
Fang
Liurong
L
Xiao
Shaobo
S
Li
Bin
B
Pan
Yongfei
Y
Luo
Rui
R
Chen
Huanchun
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D007668:Kidney / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D009500:Neutralization Tests; D014066:Palatine Tonsil / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000821:virology; D013552:Swine; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; C525999:M protein, porcine reproductive and respiratory syndrome virus; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; C114789:glycoprotein 5, PRRSV