Unprecedented, low cytotoxicity of spongelike calcium phosphate/poly(ethylene imine) hydrogel composites.
Covalently crosslinked PEI hydrogels are efficient templates for calcium phosphate mineralization in SBF. In contrast to the PEI hydrogels, non-crosslinked PEI does not lead to calcium phosphate nucleation and growth in SBF. The precipitate is a mixture of brushite and hydroxyapatite. The PEI/calcium phosphate composite material exhibits a sponge like morphology and a chemical composition that is interesting for implants. Cytotoxicity tests using Dictyostelium discoideum amoebae show that both the non-mineralized and mineralized hydrogels have a very low cytotoxicity. This suggests that next generation PEI hydrogels, where also the degradation products are non-toxic, could be interesting for biomedical applications.
Shkilnyy
Andriy
A
Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, Building 26, D-14476 Golm, Germany.
Gräf
Ralph
R
Hiebl
Bernhard
B
Neffe
Axel T
AT
Friedrich
Alwin
A
Hartmann
Jürgen
J
Taubert
Andreas
A
D000818:Animals; D001826:Body Fluids; D002130:Calcium Phosphates / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D011232:Chemical Precipitation; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004023:Dictyostelium / Q000166:cytology / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry* / Q000648:ultrastructure; D013055:Spectrophotometry, Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011094:Polyethyleneimine