[Effects of Astaxanthin on the damage of osteoblast induced by H2O2].
OBJECTIVE
To investigate the effect of Astaxanthin on enhancing the function of anti-oxidative damage in osteoblast.


METHODS
MC3T3-E1 osteoblasts were randomly divided into five groups, including control group, model group, Astaxanthin group [low-dose (1 x 10(-7) mol/L), middle-dose (1 x 10(-6) mol/L), high-dose (1 x 10(-5) mol/L)], in which the activity of cells, activity of superoxide dismutase (SOD), the content of reactive oxygen species (ROS), lipid oxygen (LPO) and membrane fluidity were tested and compared.


RESULTS
Compared with Astaxanthin groups, the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased (P < 0.01). However, the contents of ROS and LPO were significantly raised (P < 0.01).


CONCLUSIONS
H2O2 can cause oxidative damage of MC3T3-E1 osteoblasts, but Astaxanthin can prevent or decrease its influence.
Pei
Ling-peng
LP
Institute of Orthopaedics and Traumatology, China Academy of Chinese Medical Science, Beijing 100700, China.
Dong
Fu-hui
FH
Hui
Bo-di
BD
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D024341:Xanthophylls / Q000737:chemistry / Q000494:pharmacology
D000975:Antioxidants; D017382:Reactive Oxygen Species; D024341:Xanthophylls; C005948:astaxanthine; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase