title: ROS scavenging capacity and neuroprotective effect of alpha-mangostin against 3-nitropropionic acid in cerebellar granule neurons.
abstract: Alpha-mangostin is a xanthone with antioxidant properties isolated from mangosteen fruit. The reactive oxygen species (ROS) scavenging capacity and the potential protective effect of alpha-mangostin against the mitochondrial toxin 3-nitropropionic acid (3-NP) in primary cultures of cerebellar granule neurons (CGNs) were studied in the present work. It was found that alpha-mangostin was able to scavenge in a concentration-dependent way singlet oxygen, superoxide anion and peroxynitrite anion. In contrast, alpha-mangostin was unable to scavenge hydroxyl radicals and hydrogen peroxide. Furthermore, alpha-mangostin was able to ameliorate in a concentration-dependent way the neuronal death induced by 3-NP. This protective effect was associated with an amelioration of 3-NP-induced reactive oxygen species formation. It is concluded that alpha-mangostin is able to scavenge directly several ROS and has a neuroprotective effect against 3-NP in primary cultures of CGNs, which is associated with its ability to ameliorate 3-NP-induced ROS production.
authors.affiliation: Departamento de Biología, Facultad de Química, Laboratorio 209, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, México. pedraza@unam.mx
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverrí
authors.forename: Laura María
authors.initials: LM
authors.lastname: Reyes-Fermín
authors.forename: Eva Guadalupe
authors.initials: EG
authors.lastname: Nolasco-Amaya
authors.forename: Marisol
authors.initials: M
authors.lastname: Orozco-Ibarra
authors.forename: Omar Noel
authors.initials: ON
authors.lastname: Medina-Campos
authors.forename: Octavio
authors.initials: O
authors.lastname: González-Cuahutencos
authors.forename: Isabel
authors.initials: I
authors.lastname: Rivero-Cruz
authors.forename: Rachel
authors.initials: R
authors.lastname: Mata
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009574:Nitro Compounds / Q000633:toxicity; D011422:Propionates / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D009574:Nitro Compounds; D011422:Propionates; D017382:Reactive Oxygen Species; D044004:Xanthones; C015392:3-nitropropionic acid; C021053:mangostin