title: Therapeutic effects of gelatin matrix-embedded IL-12 gene-modified macrophages in a mouse model of residual prostate cancer.
abstract: We evaluated the potential use of intraoperative gelatin matrix hemostatic sealant (GMHS; FloSeal; Baxter Healthcare) embedded with macrophages (Mphi) transduced with murine interleukin (IL)-12 recombinant adenoviral vector (G/Mphi/AdmIL-12) for prevention of recurrence of prostate cancer following radical prostatectomy. Application of G/Mphi/AdmIL-12 resulted in significant suppression of tumor growth and spontaneous lung metastases, a statistically significant survival advantage of the G/Mphi/AdmIL-12-treated animals, more efficient trafficking of Mphi to lymph nodes draining from the prostate and generation of systemic natural killer cell activity and tumor-specific cytolytic T lymphocyte responses compared to the controls in a preclinical mouse model of residual prostate cancer. Our data recommend this treatment as a novel adjuvant for prevention of local recurrence of prostate cancer following radical prostatectomy.
authors.affiliation: Scott Department of Urology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: K
authors.initials: K
authors.lastname: Tabata
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Naruishi
authors.forename: K
authors.initials: K
authors.lastname: Edamura
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: E
authors.initials: E
authors.lastname: Abdel Fattah
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Goltsov
authors.forename: D
authors.initials: D
authors.lastname: Floryk
authors.forename: S D
authors.initials: SD
authors.lastname: Soni
authors.forename: D
authors.initials: D
authors.lastname: Kadmon
authors.forename: T C
authors.initials: TC
authors.lastname: Thompson
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002465:Cell Movement; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005780:Gelatin; D015316:Genetic Therapy; D006490:Hemostatics / Q000494:pharmacology; D018664:Interleukin-12 / Q000235:genetics* / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000401:mortality / Q000473:pathology / Q000628:therapy*
chemical_list: D006490:Hemostatics; D018664:Interleukin-12; D005780:Gelatin