Overexpression of the chitosanase gene in Fusarium solani via Agrobacterium tumefaciens-mediated transformation.
To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from Streptomyces hygroscopicus was used as the selection marker. Transformants which showed a significant increase in chitosanase production (approximately 2.1-fold than control) were obtained. Southern blot analysis indicated that most transformants had a single-copy T-DNA integration.
Liu
Huaiwei
H
Institute for Infocomm Research, State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda nanlu, Jinan 250100, Shandong Province, PR China.
Bao
Xiaoming
X
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D005656:Fungal Proteins; C036483:T-DNA; D006026:Glycoside Hydrolases; C019868:chitosanase