[AcSDKP attenuates PDGF-induced cardiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway].
OBJECTIVE
To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2 (ERK1/2) pathway on this process.


METHODS
Metabolic activity of fibroblasts was determined by CCK-8. Cell cycle was detected by flow cytometry. Expressions of type I and type III collagen were measured by immunocytochemistry and Western blot. Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot.


RESULTS
10(-9) mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2, while the protein levels of ERK1/2 were not significantly affected by AcSDKP.


CONCLUSIONS
AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.
Zhang
Li-juan
LJ
Experimental Center, North China Coal Medical University, Tangshan 063000, China.
Ma
Wen-dong
WD
Yang
Yan
Y
Wang
Rui-min
RM
Chen
Ping
P
Yan
Jing-bo
JB
Li
Qian
Q
Li
Dan-dan
DD
Yang
Fang
F
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D003094:Collagen; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C058504:goralatide