title: In vitro cytotoxicity of a novel injectable and biodegradable alveolar bone substitute.
abstract: The unsaturated polyphosphoester (UPPE) polymer is being investigated as an injectable and biodegradable system for alveolar bone repair in the treatment of periodontal diseases. The incorporation of beta-tricalcium phosphate (beta-TCP) particles into the UPPE polymer was previously shown to significantly increase the material's mechanical properties. Moreover, in vitro experiments demonstrated that the UPPE/beta-TCP composite was capable of zero-order release of tetracycline for over 2 weeks. In this study, we investigated the in vitro cytotoxicity of each individual component, the resulting cross-linked network and the degradation products of the UPPE/beta-TCP composite using an AlamarBlue viability assay. We confirmed that each individual component except beta-TCP and the in vitro degradation products of the composite displayed a dose-dependent cytotoxic response. Once cross-linked, however, the composite did not demonstrate an adverse response. Our results suggest that the UPPE/beta-TCP composite holds great promise for use as an injectable and biodegradable alveolar bone substitute.
authors.affiliation: Department of Orthodontics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiangli
authors.initials: X
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Xiao
authors.forename: Chenmei
authors.initials: C
authors.lastname: Liu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Qiu
mesh_terms: D020341:Absorbable Implants; D016301:Alveolar Bone Loss / Q000601:surgery; D000818:Animals; D018786:Bone Substitutes / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D010755:Organophosphates / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D011091:Polyesters / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D010755:Organophosphates; D011091:Polyesters; C485817:beta-tricalcium phosphate