title: Dental mineralization and salivary activity are reduced in offspring of spontaneously hypertensive rats (SHR).
abstract: Several pathologies have been diagnosed in children of hypertensive mothers; however, some studies that evaluated the alterations in their oral health are not conclusive. This study analyzed the salivary gland activity and dental mineralization of offsprings of spontaneously hypertensive rats (SHR). Thirty-day-old SHR males and Wistar rats were studied. The salivary flow was evaluated by injection of pilocarpine, the protein concentration and salivary amylase activity, by the Lowry method and kinetic method at 405 nm, respectively. Enamel and dentin mineralization of the mandibular incisors was quantified with aid of the microhardness meter. The results were analyzed by the ANOVA or Student's t test (p<0.05). It was noticed that the salivary flow rate (0.026 mL/min/100 g +/- 0.002) and salivary protein concentration (2.26 mg/mL +/- 0.14) of SHR offspring were reduced compared to Wistar normotensive offspring (0.036 mL/min/100 g +/- 0.003 and 2.91 mg/mL +/- 0.27, respectively), yet there was no alteration in amylase activity (SHR: 242.4 U/mL +/- 36.9; Wistar: 163.8 U/mL +/- 14.1). Microhardness was lower both in enamel (255.8 KHN +/- 2.6) and dentin (59.9 KHN +/- 0.8) for the SHR teeth compared to the Wistar teeth (enamel: 328.7 KHN +/- 3.3 and dentin: 67.1 KHN +/- 1.0). These results suggest that the SHR offspring are more susceptible to development of pathologies impairing oral health, once they presented lesser flow and salivary protein concentration and lower dental mineralization.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Araçatuba, UNESP - São Paulo State University, School of Dentistry of Araçatuba, Araçatuba, SP, Brazil.
authors.forename: Gracieli Prado
authors.initials: GP
authors.lastname: Elias
authors.forename: Otoniel Antonio Macedo
authors.initials: OA
authors.lastname: dos Santos
authors.forename: Kikue Takebayashi
authors.initials: KT
authors.lastname: Sassaki
authors.forename: Alberto Carlos Botazzo
authors.initials: AC
authors.lastname: Delbem
authors.forename: Cristina
authors.initials: C
authors.lastname: Antoniali