Selectivity in enrichment of cAMP-dependent protein kinase regulatory subunits type I and type II and their interactors using modified cAMP affinity resins.
cAMP regulates cellular functions primarily by activating PKA. The involvement of PKAs in various signaling pathways occurring simultaneously in different cellular compartments necessitates stringent spatial and temporal regulation. This specificity is largely achieved by binding of PKA to protein scaffolds, whereby a distinct group of proteins called A kinase anchoring proteins (AKAPs) play a dominant role. AKAPs are a diverse family of proteins that all bind via a small PKA binding domain to the regulatory subunits of PKA. The binding affinities between PKA and several AKAPs can be different for different isoforms of the regulatory subunits of PKA. Here we employ a combination of affinity chromatography and mass spectrometry-based quantitative proteomics to investigate specificity in PKA-AKAP interactions. Three different immobilized cAMP analogs were used to enrich for PKA and its interacting proteins from several systems; HEK293 and RCC10 cells and rat lung and testis tissues. Stable isotope labeling was used to confidently identify and differentially quantify target proteins and their preferential binding affinity for the three different cAMP analogs. We were able to enrich all four isoforms of the regulatory subunits of PKA and concomitantly identify more than 10 AKAPs. A selective enrichment of the PKA RI isoforms could be achieved; which allowed us to unravel which AKAPs bind preferentially to the RI or RII regulatory domains of PKA. Of the twelve AKAPs detected, seven preferentially bound to RII, whereas the remaining five displayed at least dual specificity with a potential preference for RI. For some of these AKAPs our data provide the first insights into their specificity.
Aye
Thin Thin
TT
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Mohammed
Shabaz
S
van den Toorn
Henk W P
HW
van Veen
Toon A B
TA
van der Heyden
Marcel A G
MA
Scholten
Arjen
A
Heck
Albert J R
AJ
D054758:A Kinase Anchor Proteins / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000737:chemistry*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis*; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007553:Isotope Labeling; D008168:Lung / Q000201:enzymology; D008745:Methylation; D008863:Microspheres; D011485:Protein Binding; D021122:Protein Subunits / Q000032:analysis*; D040901:Proteomics; D051381:Rats; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*
D054758:A Kinase Anchor Proteins; D007527:Isoenzymes; D021122:Protein Subunits; D012117:Resins, Synthetic; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D017869:Cyclic GMP-Dependent Protein Kinases