title: The Tnt1 family member Retrosol copy number and structure disclose retrotransposon diversification in different Solanum species.
abstract: Eukaryotic genome expansion/retraction caused by LTR-retrotransposon activity is dependent on the expression of full length copies to trigger efficient transposition and recombination-driven events. The Tnt1 family of retrotransposons has served as a model to evaluate the diversity among closely related elements within Solanaceae species and found that members of the family vary mainly in their U3 region of the long terminal repeats (LTRs). Recovery of a full length genomic copy of Retrosol was performed through a PCR-based approach from wild potato, Solanum oplocense. Further characterization focusing on both LTR sequences of the amplified copy allowed estimating an approximate insertion time at 2 million years ago thus supporting the occurrence of transposition cycles after genus divergence. Copy number of Tnt1-like elements in Solanum species were determined through genomic quantitative PCR whereby results sustain that Retrosol in Solanum species is a low copy number retrotransposon (1-4 copies) while Retrolyc1 has an intermediate copy number (38 copies) in S. peruvianum. Comparative analysis of retrotransposon content revealed no correlation between genome size or ploidy level and Retrosol copy number. The tetraploid cultivated potato with a cellular genome size of 1,715 Mbp harbours similar copy number per monoploid genome than other diploid Solanum species (613-884 Mbp). Conversely, S. peruvianum genome (1,125 Mbp) has a higher copy number. These results point towards a lineage specific dynamic flux regarding the history of amplification/activity of Tnt1-like elements in the genome of Solanum species.
authors.affiliation: GaTE Lab, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, IBUSP, Rua do Matão, 277, São Paulo, SP, 05508-090, Brazil.
authors.forename: M E
authors.initials: ME
authors.lastname: Manetti
authors.forename: M
authors.initials: M
authors.lastname: Rossi
authors.forename: M
authors.initials: M
authors.lastname: Nakabashi
authors.forename: M A
authors.initials: MA
authors.lastname: Grandbastien
authors.forename: Marie Anne
authors.initials: MA
authors.lastname: Van Sluys
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D018628:Gene Dosage; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D011003:Ploidies; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D032322:Solanum / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D018626:Retroelements