title: A stoichiometric model of Acidithiobacillus ferrooxidans ATCC 23270 for metabolic flux analysis.
abstract: A stoichiometric model of Acidithiobacillus ferrooxidans based on the sequenced genome from strain ATCC 23270 is derived and parameterized using genome/pathway databases. The model describes the main aspects of catabolism and anabolism. By the construction and utilization of the mathematical determination of the network, metabolic flux analysis is performed for such a bacterium for the first time and results are successfully verified by comparison to literature values. This first metabolic model of A. ferrooxidans is able to simulate the main aspects of metabolism and will be useful for further investigation and improvement of bioleaching procedures.
authors.affiliation: Department of Chemical Engineering and Biotechnology, Centre for Biochemical Engineering and Biotechnology, Millennium Institute for Cell Dynamics and Biotechnology (ICDB): a Centre for Systems Biology, University of Chile, Beauchef 861, Santiago, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Hold
authors.forename: B A
authors.initials: BA
authors.lastname: Andrews
authors.forename: J A
authors.initials: JA
authors.lastname: Asenjo
mesh_terms: D042763:Acidithiobacillus / Q000235:genetics*; D003198:Computer Simulation; D016680:Genome, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics*