title: Activation of hedgehog signaling inhibits osteoblast differentiation of human mesenchymal stem cells.
abstract: Mesenchymal stem cells within the bone are responsible for the generation of osteoblasts, chondrocytes, and adipocytes. In rodents, Indian hedgehog has been shown to play a role in osteoblast differentiation. However, evidence for a direct function of hedgehog (Hh) in human osteoblastic differentiation is missing. Using different models of human mesenchymal stem cells we show that Hh signaling decreases during osteoblast differentiation. This is associated with a decrease in Smoothened expression, a key partner that triggers Hh signaling, and in the number of cells displaying a primary cilium, an organelle necessary for Hh signaling. Remarkably, treatment of human mesenchymal stem cells with sonic hedgehog or two molecules able to activate Hh signaling inhibits osteoblast differentiation. This inhibition is visualized through a decrease in mineralization and in the expression of osteoblastic genes. In particular, activation of Hh signaling induces a decrease in Runx2 expression, a key transcriptional factor controlling the early stage of osteoblast differentiation. Consistently, the activation of Hh signaling during the first days of differentiation is sufficient to inhibit osteoblast differentiation, whereas differentiated osteoblasts are not affected by Hh signaling. In summary, we show here, using various inducers of Hh signaling and mesenchymal stem cells of two different origins, that Hh signaling inhibits human osteoblast differentiation, in sharp contrast to what has been described in rodent cells. This species difference should be taken into account for screening for pro-osteogenic molecules.
authors.affiliation: Institute of Signaling, Biology, Development and Cancer, Université de Nice Sophia-Antipolis, CNRS UMR, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Plaisant
authors.forename: Coralie
authors.initials: C
authors.lastname: Fontaine
authors.forename: Wendy
authors.initials: W
authors.lastname: Cousin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rochet
authors.forename: Christian
authors.initials: C
authors.lastname: Dani
authors.forename: Pascal
authors.initials: P
authors.lastname: Peraldi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005786:Gene Expression Regulation; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D009025:Morpholines / Q000494:pharmacology; D055611:Natural Killer T-Cells; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011687:Purines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D017077:Culture Media, Conditioned; D053823:Hedgehog Proteins; D006888:Hydroxycholesterols; D009025:Morpholines; D011687:Purines; C509239:SHH protein, human; D000469:Alkaline Phosphatase; C470893:purmorphamine