Parvovirus capsid disorders cholesterol-rich membranes.
In this study canine parvovirus, CPV, was found to induce disorder in DPPC:cholesterol membranes in acidic conditions. This acidicity-induced fluidizing effect is suggested to originate from the N-terminus of the viral capsid protein VP1. In accordance with the model membrane studies, a fluidizing effect was seen also in the endosomal membranes during CPV infection implying an important functional role of the fluidization in the endocytic entry of the virus.
Pakkanen
Kirsi
K
Department of Biological and Environmental Science and Nanoscience Center, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland. kirsi.i.pakkanen@jyu.fi
Kirjavainen
Sanna
S
Mäkelä
Anna R
AR
Rintanen
Nina
N
Oker-Blom
Christian
C
Jalonen
Tuula O
TO
Vuento
Matti
M
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D017993:Parvovirus, Canine / Q000737:chemistry*
D036022:Capsid Proteins; D008567:Membranes, Artificial; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol