Antitumor and antioxidant activity of protocatechualdehyde produced from Streptomyces lincolnensis M-20.
We characterized the biological functions of protocatechualdehyde (PA) isolated from the butanol extract of culture supernatant from Streptomyces lincolnensis M-20. Following butanol extraction, it was purified by silica gel and Sephadex LH-20 column chromatography. PA was analyzed by Furier Transform Infrared Spectroscopy (FT-IR), Gas chromatograph-Mass Spectrometer (GC-MS), and Nuclear Magnetic Resonance (NMR). PA had potent antioxidant activity, as measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Antitumor activity against MCF-7 human breast cancer cells was evaluated by the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide (MTT) assay. PA treatment (0 approximately 150 muM) dose-dependently blocked apoptosis, as shown by improved cell viability and inter-nucleosomal DNA fragmentation. Our findings suggest that Streptomyces lincolnensis M-20, a lincomycin producer, also produces protocatechualdehyde.
Kim
Kyoung-Ja
KJ
Department of Biotechnology, College of Life Science, Soonchunhyang University, Asan, 336-745, Korea. kyoungjakim@hotmail.com.
Kim
Mi-Ae
MA
Jung
Jee-Hyung
JH
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001547:Benzaldehydes / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D053938:DNA Fragmentation; D003911:Dextrans; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010851:Picrates / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D001547:Benzaldehydes; D001713:Biphenyl Compounds; D002396:Catechols; D003911:Dextrans; D010851:Picrates; C005581:protocatechualdehyde; C025614:sephadex; C004931:1,1-diphenyl-2-picrylhydrazyl