title: A method for identification of HIV gp140 binding memory B cells in human blood.
abstract: Antibodies to HIV are potentially important reagents for basic and clinical studies. Historically, these reagents have been produced by random cloning of heavy and light chains in phage display libraries [Burton, D.R., Barbas, C.F. III, Persson, M.A.A., Koenig, S., Chanock, R.M., and Lerner, R.A., (1991), A large array of human monoclonal antibodies to type 1 immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. U. S. A. 88, 10134-10137.] and electrofusion techniques [Buchacher, A., Predl, R., Tauer, C., Purtscher, M., Gruber, G., Heider, R., Steindl, F., Trkola, A., Jungbauer, A., and Katinger, H., (1992), Human monoclonal antibodies against gp41 and gp120 as potential agent for passive immunization. Vaccines 92, 191-195]. Here we describe a method to identify and potentially enrich human memory B cells from HIV infected patients that show serum titers of neutralizing antibodies. When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry.
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA.
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Scheid
authors.forename: Hugo
authors.initials: H
authors.lastname: Mouquet
authors.forename: Niklas
authors.initials: N
authors.lastname: Feldhahn
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Walker
authors.forename: Florencia
authors.initials: F
authors.lastname: Pereyra
authors.forename: Emily
authors.initials: E
authors.lastname: Cutrell
authors.forename: Michael S
authors.initials: MS
authors.lastname: Seaman
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
authors.forename: Richard T
authors.initials: RT
authors.lastname: Wyatt
authors.forename: Hedda
authors.initials: H
authors.lastname: Wardemann
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D019921:Biotinylation; D005434:Flow Cytometry; D015483:HIV Antibodies / Q000097:blood* / Q000276:immunology; D015658:HIV Infections / Q000097:blood / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D008297:Male; D009500:Neutralization Tests; D054299:env Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1