Direct production of L-tagatose from L-psicose by Enterobacter aerogenes 230S.
L-tagatose was produced directly from L-psicose by subjecting the same biomass suspension to microbial reduction followed by oxidation using a newly isolated bacteria Enterobacter aerogenes 230S. After various optimizations, it was observed that cells grown on xylitol have the best conversion potential. Moreover, E. aerogenes 230S converted L-psicose to L-tagatose at a faster rate in the presence of polyols such as glycerol, D-sorbitol, ribitol, L-arabitol, D-mannitol and xylitol. At 5% substrate concentration, the conversion ratio of L-psicose to L-tagatose was above 60% in the presence of glycerol. Identity of crystalline L-tagatose was confirmed by HPLC analysis, (13)C-NMR spectra, and optical rotation.
Rao
Devendar
D
Rare Sugar Research Center, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, Japan.
Gullapalli
Pushpakiran
P
Yoshihara
Akihide
A
Jenkinson
Sarah F
SF
Morimoto
Kenji
K
Takata
Goro
G
Akimitsu
Kazuya
K
Tajima
Shigeyuki
S
Fleet
George W J
GW
Izumori
Ken
K
D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D021902:Enterobacter aerogenes / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D013402:Sugar Alcohols / Q000378:metabolism*
D002241:Carbohydrates; D006601:Hexoses; D013402:Sugar Alcohols; C003243:psicose; D005632:Fructose; C042890:altritol; D002244:Carbon; D010100:Oxygen; C030192:tagatose