Protein fractionation according to molecular size in constant pH media with immobilized charges in colinear porosity gradient.
A new method of protein electrophoresis is described here. Electrophoretic separation is performed in gel media with uniform concentration of immobilized charges, combined with porosity gradient directed against protein movement. Successful separation becomes possible due to the effect of strong sample zone compression; the latter effect is connected with complex conductivity profile dynamics in a gel system containing immobilized charges. Immobilized buffers combined with porosity gradient provide an opportunity of protein discrimination based on molecular size, while in the case of uniform gel concentration the separation is based on mobility differences and strongly affected by non-uniform electric field strength profile. The proposed method does not require ionic detergent for protein separation according to their molecular weight.
Stoyanov
Alexander V
AV
Research Institute for Children, Children's Hospital, New Orleans, LA 70118, USA. stoyanov@ufl.edu
D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D012107:Research Design
D011506:Proteins