[Study of inducing bone marrow-derived mesenchymal stem cells into chondrocytes in vitro].
OBJECTIVE
To explore a method of isolation, culture and chondrogenic phenotype differentiation of mesenchymal stem cell (MSCs) from the bone marrow of rats in vitro and to offer experimental reference for the resources of seeding cells in cartilage tissue engineering.


METHODS
MSCs were isolated from bone marrow and purified by density gradient centrifuge and cultured in vitro. The MSC adherence formed and those in passage 3 were chosen to induce into chondrogenic differentiation. After 7, 14, 21 days, immunohistochemical techique was applied to detect the expression of collagen type II. The differentiated cells were implanted on the CPP/PLLA composites. After the cell-scaffold complex was cultured in vitro for one week, the ultrastructure of the scaffold was observed with scanning electron microscopy.


RESULTS
The differentiated cells changed from a spindle-like fibroblastic appearance to a polygonal shape, the capability of proliferation was down markedly. Immunohistochemical staining of collagen II were positive for the pass age, especially in the 21st days. Induced MSCs were well adherent to the scaffold composites and the cells were embedded by the cell-matrix.


CONCLUSIONS
Under the induced medium, MSCs can differentiate into chondrogenic phenotype and secrete specificity matrix of cartilage in vitro. MSCs can likely be served as optimal cell source for cartilage tissue engineering.
Tong
Zhi-Chao
ZC
Department of Orthopaedics, Xi'an Red Cross Society Hospital, Xián 710054, Shaanxi, China. zhichaotong@126.com
Yang
Zhen
Z
Tong
Zhi-Qin
ZQ
Wang
Kun-Zheng
KZ
Yang
Tuan-Min
TM
Shi
Zong-Li
ZL
Li
Yi
Y
Guo
Xiong
X
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation; D002469:Cell Separation; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology; D020219:Chondrogenesis; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds