title: Rapid diagnosis of tuberculous pleuritis by a T-cell interferon-gamma release assay.
abstract: To obtain a correct diagnosis of tuberculous pleuritis by analysis of pleural effusions with standard diagnostic tools is difficult. Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunosorbent assay (ELISA) or MTB-specific enzyme-linked immunospot assay (ELISpot) on peripheral blood was not useful because the judgment was indeterminate or negative. However, we recently encountered 2 cases in which tuberculous pleuritis was rapidly diagnosed by performing MTB-specific ELISpot on pleural effusion mononuclear cells (PEMCs), because high levels of MTB-specific cells by ELISpot were demonstrated in pleural effusion of patients with tuberculous pleuritis. to analyze MTB-specific T-cells in pleural effusion mononuclear cells by ELISpot is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of tuberculous pleuritis.
authors.affiliation: Division of Respiratory Diseases, Department of Medicine, Kawasaki Medical School, Kurashiki, Japan. yoshihiro@med.kawasaki-m.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kobashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Keiji
authors.initials: K
authors.lastname: Mouri
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Obase
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miyashita
authors.forename: Mikio
authors.initials: M
authors.lastname: Oka
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000032:analysis* / Q000378:metabolism; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*; D014396:Tuberculosis, Pleural / Q000175:diagnosis* / Q000188:drug therapy
chemical_list: D000995:Antitubercular Agents; D007371:Interferon-gamma