Inhibition of laryngeal cancer cell invasion and growth with lentiviral-vector delivered short hairpin RNA targeting human MMP-9 gene.
The purpose of this study was to explore the inhibiting role of MMP-9 gene silence in the invasive ability and growth of laryngeal squamous cell carcinoma (LSCC) by lentivirus mediated RNA interference. MMP-9-RNAi-lentivirus and the control lentivirus (GFP-lentivirus) were transfected into Hep-2 cells. Gelatin zymography showed the proteins expression of MMP-9 were knockdown in the MMP-9 siRNA transfected Hep-2 cells. The invasive activity and viability of MMP-9 siRNA treated Hep-2 cells were decreased than the control cells measured with modified Boyden chamber assay and MTT assay. In animal experiment, 20 nude mice bearing Hep-2 cell tumor were randomly separated into the experimental and the control groups. The former were intratumorally injected with MMP-9-RNAi-lentivirus, and the later were injected with equivalent dose of GFP-lentivirus. Results showed the average weight and volume of tumor in MMP-9-RNAi-lentivirus treated group were significantly lower than those in the control group (P < .01). The protein expressions of MMP-9 were downregulated in tumors of MMP-9-RNAi-lentivirus treatment. The PCNA index was obviously lower in the tumors of treated group than that in the control group (P < .01). These results suggest that MMP-9 gene silence by lentivirus mediated RNA interference can inhibit invasion and growth of LSCC.
Sun
Yanan
Y
The Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Liu
Ming
M
Yang
Baofeng
B
Lu
Jianguang
J
Li
Baoxin
B
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D016086:Lentivirus / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D016337:RNA, Catalytic / Q000235:genetics*; D014162:Transfection
D016337:RNA, Catalytic; D020780:Matrix Metalloproteinase 9