Association of parental hyperhomocysteinemia and C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism with recurrent pregnancy loss.
OBJECTIVE
To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL).


METHODS
A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to assess total plasma homocysteine, C677T MTHFR polymorphism and DNA damage respectively. Student t-test, ANOVA and Fisher exact test were used for statistical analysis.


RESULTS
Maternal [mean: 11.6+/-5.0 versus 8.6+/-4.2 micromol/L, odds ratio (OR): 4.48] and paternal [mean: 19.6+/-9.5 versus 14.2+/-7.4 micromol/L, OR: 6.92] hyperhomocysteinemia, paternal age [OR: 1.16], paternal MTHFR 677T allele [OR: 2.30] and DNA damage were found to increase the risk for RPL. DNA damage showed positive correlation with plasma homocysteine and MTHFR 677T allele.


CONCLUSIONS
Parental hyperhomocysteinemia, paternal age, paternal C677T MTHFR polymorphism and DNA damage are risk factors for RPL. DNA damage showed positive correlation with plasma homocysteine and MTHFR 677T allele.
Govindaiah
Vinukonda
V
Dept. of pathology, New York Medical College, Valhalla, NY 10595, USA.
Naushad
Shaik Mohammad
SM
Prabhakara
Krishnamurthy
K
Krishna
Prasad Chintakindi
PC
Radha Rama Devi
Akella
A
D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution / Q000235:genetics; D016022:Case-Control Studies; D004249:DNA Damage; D020964:Embryo Loss / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006710:Homocysteine / Q000097:blood; D006801:Humans; D020138:Hyperhomocysteinemia / Q000201:enzymology* / Q000235:genetics*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D048629:Micronuclei, Chromosome-Defective; D010290:Parents; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D012008:Recurrence; D055815:Young Adult
D006710:Homocysteine; D042965:Methylenetetrahydrofolate Reductase (NADPH2)