title: Repeated ethanol exposure during late gestation alters the maturation and innate immune status of the ovine fetal lung.
abstract: Little is known about the effects of fetal ethanol exposure on lung development. Our aim was to determine the effects of repeated ethanol exposure during late gestation on fetal lung growth, maturation, and inflammatory status. Pregnant ewes were chronically catheterized at 91 days of gestational age (DGA; term approximately 147 days). From 95-133 DGA, ewes were given a 1-h daily infusion of either 0.75 g ethanol/kg (n = 9) or saline (n = 8), with tissue collection at 134 DGA. Fetal lungs were examined for changes in tissue growth, structure, maturation, inflammation, and oxidative stress. Between treatment groups, there were no differences in lung weight, DNA and protein contents, percent proliferating and apoptotic cells, tissue and air-space fractions, alveolar number and mean linear intercept, septal thickness, type-II cell number and elastin content. Ethanol exposure caused a 75% increase in pulmonary collagen I alpha1 mRNA levels (P < 0.05) and a significant increase in collagen deposition. Surfactant protein (SP)-A and SP-B mRNA levels were approximately one third of control levels following ethanol exposure (P < 0.05). The mRNA levels of the proinflammatory cytokines interleukin (IL)-1beta and IL-8 were also lower (P < 0.05) in ethanol-exposed fetuses compared with controls. Pulmonary malondialdehyde levels tended to be increased (P = 0.07) in ethanol-exposed fetuses. Daily exposure of the fetus to ethanol during the last third of gestation alters extracellular matrix deposition and surfactant protein gene expression, which could increase the risk of respiratory distress syndrome after birth. Changes to the innate immune status of the fetus could increase the susceptibility of the neonatal lungs to infection.
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia. foula.sozo@med.monash.edu.au
authors.forename: Foula
authors.initials: F
authors.lastname: Sozo
authors.forename: Luke
authors.initials: L
authors.lastname: O'Day
authors.forename: Gert
authors.initials: G
authors.lastname: Maritz
authors.forename: Kelly
authors.initials: K
authors.lastname: Kenna
authors.forename: Victoria
authors.initials: V
authors.lastname: Stacy
authors.forename: Nadine
authors.initials: N
authors.lastname: Brew
authors.forename: David
authors.initials: D
authors.lastname: Walker
authors.forename: Alan
authors.initials: A
authors.lastname: Bocking
authors.forename: James
authors.initials: J
authors.lastname: Brien
authors.forename: Richard
authors.initials: R
authors.lastname: Harding
mesh_terms: D000818:Animals; D001483:Base Sequence; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004549:Elastin / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D005325:Fetal Organ Maturity / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D005333:Fetus / Q000187:drug effects* / Q000276:immunology*; D015870:Gene Expression / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects* / Q000235:genetics; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000196:embryology / Q000276:immunology* / Q000378:metabolism; D008431:Maternal-Fetal Exchange / Q000276:immunology*; D018384:Oxidative Stress; D011247:Pregnancy; D037661:Pulmonary Surfactant-Associated Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012756:Sheep
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D037661:Pulmonary Surfactant-Associated Proteins; D012333:RNA, Messenger; D000431:Ethanol; D003094:Collagen; D004549:Elastin