title: Left handed beta helix models for mammalian prion fibrils.
abstract: We propose models for in vitro grown mammalian prion protein fibrils based upon left handed beta helices formed both from the N-terminal and C-terminal regions of the proteinase resistant infectious prion core. The C-terminal threading onto a beta-helical structure is almost uniquely determined by fixing the cysteine disulfide bond on a helix corner. In comparison to known left handed helical peptides, the resulting model structures have similar stability attributes including relatively low root mean square deviations in all atom molecular dynamics, substantial side-chain-to-side-chain hydrogen bonding, good volume packing fraction, and low hydrophilic/hydrophobic frustration. For the N-terminus, we propose a new threading of slightly more than two turns, which improves upon the above characteristics relative to existing three turn beta-helical models. The N-terminal and C-terminal beta helices can be assembled into eight candidate models for the fibril repeat units, held together by large hinge (order 30 residues) domain swapping, with three amenable to fibril promoting domain swapping via a small (five residue) hinge on the N-terminal side. Small concentrations of the metastable C-terminal beta helix in vivo might play a significant role in templating the infectious conformation and in enhancing conversion kinetics for inherited forms of the disease and explain resistance (for canines) involving hypothesized coupling to the methionine 129 sulfur known to play a role in human disease.
authors.affiliation: Department of Physics, University of California, Davis, California 95616, USA.
authors.forename: Kay C
authors.initials: KC
authors.lastname: Kunes
authors.forename: Scott C
authors.initials: SC
authors.lastname: Clark
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Cox
authors.forename: Rajiv R P
authors.initials: RR
authors.lastname: Singh
mesh_terms: D004220:Disulfides / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D011328:Prions / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D004220:Disulfides; D011328:Prions