title: Bone morphogenetic protein 7 (BMP-7) increases the expression of follicle-stimulating hormone (FSH) receptor in human granulosa cells.
abstract: OBJECTIVE
  To examine the effect of bone morphogenetic protein 7 (BMP-7) on FSH receptor (FSHR) expression in human granulosa cells.
  
  
  METHODS
  Laboratory study using human samples.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Human granulosa cells were obtained from 60 women undergoing oocyte retrieval for IVF.
  
  
  METHODS
  Human granulosa cells (GCs) were cultured with recombinant BMP-7, followed by RNA extraction.
  
  
  METHODS
  mRNA levels of GCs were measured by real-time reverse-transcription polymerase chain reaction.
  
  
  RESULTS
  Bone morphogenetic protein 7 increased FSHR gene expression in human luteinized granulosa cells, whereas it decreased LH receptor gene expression. Bone morphogenetic protein 7 also increased FSH-induced cyclic adenosine monophosphate production in GCs, indicating up-regulation of the cellular response to FSH. Although BMP-7 increased gene expression of activin-betaA and -betaB in GCs, inhibition of activin function did not affect the BMP-7-induced FSHR gene expression.
  
  
  CONCLUSIONS
  These findings provide new insight into the biologic function of BMP-7 in the human ovary and demonstrate its unique mechanism of regulating FSHR action.
authors.affiliation: Department of Obstetrics and Gynecology, University of Tokyo, Tokyo 113-8655, Japan.
authors.forename: Jia
authors.initials: J
authors.lastname: Shi
authors.forename: Osamu
authors.initials: O
authors.lastname: Yoshino
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Osuga
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishii
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000502:physiology*; D052004:Bone Morphogenetic Protein Receptors / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D011962:Receptors, FSH / Q000096:biosynthesis* / Q000235:genetics; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics
chemical_list: D055419:Bone Morphogenetic Protein 7; D012333:RNA, Messenger; D011962:Receptors, FSH; D011974:Receptors, LH; D052004:Bone Morphogenetic Protein Receptors