Normal breathing pattern and arterial blood gases in awake and sleeping goats after near total destruction of the presumed pre-Botzinger complex and the surrounding region.
Abrupt neurotoxic destruction of >70% of the pre-Bötzinger complex (preBötzC) in awake goats results in respiratory and cardiac failure (Wenninger JM, Pan LG, Klum L, Leekley T, Bastastic J, Hodges MR, Feroah TR, Davis S, Forster HV. J Appl Physiol 97: 1629-1636, 2004). However, in reduced preparations, rhythmic respiratory activity has been found in other areas of the brain stem (Huang Q, St. John WM. J Appl Physiol 64: 1405-1411, 1988; Janczewski WA, Feldman JL. J Physiol 570: 407-420, 2006; Lieske SP, Thoby-Brisson M, Telgkamo P, Ramierz JM. Nature Neurosci 3: 600-607, 2000; St. John WM, Bledsoe TA. J Appl Physiol 59: 684-690, 1985); thus we hypothesized that, when the preBötzC is destroyed incrementally over weeks, time-dependent plasticity within the respiratory network will result in a respiratory rhythm capable of maintaining normal blood gases. Microtubules were bilaterally implanted into the presumed preBötzC of seven goats. After recovery from surgery, studies were completed to establish baseline values for respiratory parameters. At weekly intervals, increasing volumes (in order 0.5, 1, 5, and 10 microl) of ibotenic acid (IA; 50 mM) were then injected into the preBötzC. All IA injections resulted in an acute tachypnea and dysrhythmia featuring augmented breaths, apneas, and increased breath-to-breath variation in breathing. In studies at night, apneas were nearly all central and occurred in the awake state. Breath-to-breath variation in breathing was greater (P < 0.05) during wakefulness than during non-rapid eye movement sleep. However, one week after the final IA injection, the breathing pattern, breath-to-breath variation, and arterial blood gases and pH were unchanged from baseline, but there was a 20% decrease in respiratory frequency (f) and CO(2) sensitivity (P < 0.05), as well as a 40% decrease in the ventilatory response to hypoxia (P < 0.001). In subsequent histological analysis of the presumed preBötzC region of lesioned goats, it was determined that there was a 90 and 92% reduction from control goats in total and neurokinin-1 receptor neurons, respectively. Therefore, it was concluded that 1) the dysrhythmic effects on breathing are state dependent; and 2) after incremental, near total destruction of the presumed preBötzC region, time-dependent plasticity within the respiratory network provides a rhythm capable of sustaining normal arterial blood gases.
Krause
K L
KL
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA. klkrause@mcw.edu
Forster
H V
HV
Kiner
T
T
Davis
S E
SE
Bonis
J M
JM
Qian
B
B
Pan
L G
LG
D000222:Adaptation, Physiological; D000818:Animals; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000097:blood*; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage; D005260:Female; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007051:Ibotenic Acid / Q000008:administration & dosage; D007267:Injections; D023421:Models, Animal; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D009473:Neuronal Plasticity; D010100:Oxygen / Q000097:blood*; D010507:Periodicity; D012123:Pulmonary Ventilation; D012125:Respiratory Center / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D015656:Respiratory Mechanics; D012890:Sleep; D013997:Time Factors; D014851:Wakefulness
D018690:Excitatory Amino Acid Agonists; D002245:Carbon Dioxide; D007051:Ibotenic Acid; D010100:Oxygen