title: Ultrasonic and electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto a titanium plasma-spray surface.
abstract: Several studies suggest that the surface coating of titanium could play an important role in bone tissue engineering. In the present study, we have followed a particular biomimetic strategy where ultrasonically or electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix on a titanium plasma-spray surface. In comparison with control conditions, the ultrasonic stimulation (average power, 149 mW; frequency, 1.5 MHz) and the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation, and increased surface coating with decorin, osteocalcin, osteopontin, and type I collagen together with higher incorporation of calcium and phosphorus inside the extracellular matrix. The immunofluorescence related to the preceding bone matrix proteins showed their colocalization in the cell-rich areas. The use of the two physical stimulations aimed at obtaining the coating of the rough titanium plasma-spray surface in terms of cell colonization and deposition of extracellular matrix. The superficially cultured biomaterial could be theoretically used, in clinical applications, as an implant for bone repair.
authors.affiliation: Dipartimento di Informatica e Sistemistica, Università di Pavia, Pavia, Italy. lorenzo.fassina@unipv.it
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fassina
authors.forename: Enrica
authors.initials: E
authors.lastname: Saino
authors.forename: Maria Sonia
authors.initials: MS
authors.lastname: Sbarra
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Cusella De Angelis
authors.forename: Giuliano
authors.initials: G
authors.lastname: Mazzini
authors.forename: Francesco
authors.initials: F
authors.lastname: Benazzo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Magenes
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D055590:Electromagnetic Phenomena; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium / Q000378:metabolism*; D014465:Ultrasonics
chemical_list: D016326:Extracellular Matrix Proteins; D014025:Titanium