title: Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci.
abstract: BACKGROUND
  Three phenotypic identification systems (MicroScan, VITEK 2, and Crystal GP) were evaluated for their accuracy to identify coagulase-negative staphylococci (CNS). A total of 120 clinical isolates confirmed to be CNS via 16S rRNA sequencing and analysis with the MicroSeq 500 v2.0 database were assessed.
  
  
  RESULTS
  The MicroScan, VITEK 2, and Crystal GP systems correctly identified 82.5%, 87.5%, and 67.5% of the isolates, respectively. Misidentification was the main problem in MicroScan (10.8%) and Crystal GP (23.3%) systems, whereas the main problem of VITEK 2 was low-level discrimination (7.5%).
  
  
  CONCLUSIONS
  None of the 3 phenotypic systems tested could accurately and reliably identify CNS at the species level. Further verifications such as biochemical testing or 16S rRNA sequencing together with analysis using a comparable database might be helpful in this regard.
authors.affiliation: Department of Laboratory Medicine, Seoul National University Hospital, 101 Daehang-no, Jongno-gu, Seoul, South Korea. rabbit790622@gmail.com
authors.forename: Miyoung
authors.initials: M
authors.lastname: Kim
authors.forename: Se Ran
authors.initials: SR
authors.lastname: Heo
authors.forename: Soon Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Hyelin
authors.initials: H
authors.lastname: Kwon
authors.forename: Jeong Su
authors.initials: JS
authors.lastname: Park
authors.forename: Moon-Woo
authors.initials: MW
authors.lastname: Seong
authors.forename: Do-Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Kyoung Un
authors.initials: KU
authors.lastname: Park
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.forename: Eui-Chong
authors.initials: EC
authors.lastname: Kim
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards*; D003030:Coagulase / Q000378:metabolism; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
chemical_list: D003030:Coagulase; D012336:RNA, Ribosomal, 16S