title: A role for the host coatomer and KDEL receptor in early vaccinia biogenesis.
abstract: Members of the poxvirus family have been investigated for their applications as vaccines and expression vectors and, more recently, because of concern for their potential as biological weapons. Vaccinia virus, the prototypic member, evolves through multiple forms during its replication. Here, we show a surprising way by which vaccinia hijacks coatomer for early viral biogenesis. Whereas coatomer forms COPI vesicles in the host early secretory system, vaccinia formation bypasses this role of coatomer, but instead, depends on coatomer interacting with the host KDEL receptor. To gain insight into the viral roles of these two host proteins, we have detected them on the earliest recognized viral forms. These findings not only suggest insights into early vaccinia biogenesis but also reveal an alternate mechanism by which coatomer acts.
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Leiliang
authors.initials: L
authors.lastname: Zhang
authors.forename: Stella Y
authors.initials: SY
authors.lastname: Lee
authors.forename: Galina V
authors.initials: GV
authors.lastname: Beznoussenko
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Peters
authors.forename: Jia-Shu
authors.initials: JS
authors.lastname: Yang
authors.forename: Hui-ya
authors.initials: HY
authors.lastname: Gilbert
authors.forename: Abraham L
authors.initials: AL
authors.lastname: Brass
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elledge
authors.forename: Stuart N
authors.initials: SN
authors.lastname: Isaacs
authors.forename: Bernard
authors.initials: B
authors.lastname: Moss
authors.forename: Alexander
authors.initials: A
authors.lastname: Mironov
authors.forename: Victor W
authors.initials: VW
authors.lastname: Hsu
mesh_terms: D000818:Animals; D016466:CHO Cells; D022181:COP-Coated Vesicles; D020756:Coatomer Protein / Q000378:metabolism / Q000502:physiology*; D006224:Cricetinae; D003412:Cricetulus; D006678:HIV / Q000235:genetics / Q000502:physiology; D018000:Receptors, Peptide / Q000378:metabolism / Q000502:physiology*; D014616:Vaccinia virus / Q000235:genetics*; D014779:Virus Replication
chemical_list: D020756:Coatomer Protein; C064459:KDEL receptor; D018000:Receptors, Peptide