title: The distribution of kisspeptin and its receptor GPR54 in rat dorsal root ganglion and up-regulation of its expression after CFA injection.
abstract: Kisspeptin/GPR54 system plays a crucial role in the control of puberty onset and reproductive function. In the present study, we gave the first report that kisspeptin and GPR54 were expressed in the small- to large-sized neurons, and co-localized with Bandeiraea simplicifolia isolectin B4 (IB4), calcitonin-gene-related peptide (CGRP) and neurofilament 200 (NF200) in the L4/5 dorsal root ganglion (DRG) of naïve rats, detected by the double immunofluorescent staining. Interestingly, a marked elevation in the levels of KiSS-1 and GPR54 mRNA as well as protein was observed in the spinal dorsal horn and DRG 4 and 14 days following intra-articular injection of complete Freund's adjuvant (CFA), indicating a possible involvement of the kisspeptin/GPR54 system in chronic inflammatory pain.
authors.affiliation: Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, Shanghai 20032, China. wu003@shmu.edu.cn
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Mi
authors.forename: Qi-Liang
authors.initials: QL
authors.lastname: Mao-Ying
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Xiu
authors.initials: X
authors.lastname: Li
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Gen-Cheng
authors.initials: GC
authors.lastname: Wu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000633:toxicity; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006614:Hindlimb / Q000187:drug effects / Q000503:physiopathology; D006930:Hyperalgesia / Q000139:chemically induced / Q000503:physiopathology; D007150:Immunohistochemistry; D007270:Injections, Intra-Articular; D059648:Kisspeptins; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010147:Pain Measurement / Q000379:methods; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D000074502:Receptors, Kisspeptin-1; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: C491406:Kiss1 protein, rat; C492247:Kiss1r protein, rat; D059648:Kisspeptins; D016900:Neurofilament Proteins; D011506:Proteins; D043562:Receptors, G-Protein-Coupled; D000074502:Receptors, Kisspeptin-1; C051774:neurofilament protein H; D005620:Freund's Adjuvant; D015740:Calcitonin Gene-Related Peptide