title: Triterpenoic acids of Prunella vulgaris var. lilacina and their cytotoxic activities in vitro.
abstract: The column chromatographic separation of the MeOH extract from the aerial parts of Prunella vulgaris var. lilacina Nakai led to the isolation of fifteen triterpenoic acids (2-6, 9-13, 16-20), four flavonoids (14, 21-23), four phenolics (7, 8, 15, 24), and a diterpene (1). Their structures were determined by spectroscopic methods to be trans-phytol (1), oleanic acid (2) ursolic acid (3), 2alpha,3alpha,19alpha-trihydroxyurs-12en-28oic acid (4), 2alpha,3alpha-dihydroxyurs-12en-28oic acid (5), maslinic acid (6), caffeic acid (7), phydroxy cinnamic acid (8), 2alpha,3alpha,19alpha,23-tetrahydroxyurs-12en-28oic acid (9), 2alpha,3alpha,23-trihydroxyurs-12en-28oic acid (10), 2alpha,3beta-dihydroxyurs-12en-28oic acid (11), 2alpha,3beta,24-trihydroxyolea-12en-28oic acid (12), (12R, 13S)-2alpha,3alpha,24,trihydroxy-12,13-cyclo-taraxer-14-en-28oic acid (13), quercertin 3-O-beta-D-glucopyranoside (14), rosmarinic acid (15), 2alpha,3alpha,24-trihydroxyurs-12,20(30)-dien-28oic acid (16), 2alpha,3alpha,24-trihydroxyolea-12en-28oic acid (17), 2alpha,3beta,19alpha,24-tetrahydroxyurs-12en-28oic acid 28-O-Dglucopyranoside (18), 2alpha,3alpha,19alpha,24-tetrahydroxyurs-12en-28oic acid 28-O-D-glucopyranoside (19), prunvuloside A (20), kaempferol 3-O-alpha-L-rhamnopyranosyl(1-->6)-beta-D-glucopranoside (21), kaempferol 3-O-beta-D-glucopyranoside (22), quercertin 3-O-alpha-L-rhamnopyranosyl(1-->6)-beta-D-glucopyranoside (23), and 2-hydroxy-3-(3',4'-dihydroxyphenly)propanoic acid (24). Compounds 1, 8-12, 17, 21, 23, and 24 were isolated from this plant source for the first time. The isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells in vitro using the sulforhodamin B bioassay (SRB) method. Compound 3 exhibited moderate cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells, with ED(50) values of 3.71, 3.65, 13.62, and 5.44 microM, respectively.
authors.affiliation: Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea. krlee@skku.ac.kr
authors.forename: Il Kyun
authors.initials: IK
authors.lastname: Lee
authors.forename: Do Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Seung Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyung Ran
authors.initials: KR
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Jong Ki
authors.initials: JK
authors.lastname: Hong
authors.forename: Jei Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Park
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D031347:Prunella / Q000737:chemistry*; D012235:Rhodamines; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D007202:Indicators and Reagents; D012235:Rhodamines; D014315:Triterpenes; C022027:lissamine rhodamine B