Depletion of high-abundance serum proteins from human uterine lavages enhances detection of lower-abundance proteins.
Two-dimentional electrophoresis, 2-DE, of human uterine lavages demonstrated the abundance of serum proteins. Two methods of serum protein removal were assessed. Multiple affinity removal system was most effective in depleting abundant serum proteins. 2D-DiGE comparing depleted versus nondepleted lavages resulted in increased protein detection following depletion, further enhanced by assessing the protein profile over a narrower pI 4-7 range. Demonstrating depletion of abundant serum proteins is important for sensitive proteomic analysis.
Hannan
Natalie J
NJ
Department of Obstetrics and Gynecology, Monash University, Clayton, 3168 Australia. natalie.hannan@princehenrys.org
Stoikos
Chelsea J
CJ
Stephens
Andrew N
AN
Salamonsen
Lois A
LA
D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D020543:Proteome / Q000032:analysis; D007507:Therapeutic Irrigation; D014599:Uterus / Q000737:chemistry*
D001798:Blood Proteins; D020543:Proteome