[Role of pilose antler polypeptides on replicative senescence of rat chondrocyte].
OBJECTIVE
To observe the function of pilose antler polypeptides (PAP) on replicative senescence of rat chondrocyte subcultivated in vitro by means of PAP intervention and controlled experiment.


METHODS
The 3rd generation chondrocytes were divided into blank group, and PAP groups with three different concentration of PAP which were passaged to the 4th generation. Meanwhile, the 2nd generation of chontrocytes were used as control group. The chondrocytes in different groups were detected with the method of histochemistry for S-A-beta-gal, flow cytometry for cell life cycle and proliferation index, alcian blue test for the content and structure of GAG of ECM, and RT-PCR for type II collagen and Aggrecan. Then PAP's function was observed regarding the appearance and functional status in the process of chondrocyte's senescence.


RESULTS
PAP significantly inhibited chondrocyte's express of S-A-beta-gal (P<0.01), promoted chondrocyte's proliferation (P<0.01), reduced cell content on G1 phase, enhanced the content of GAG, type II collagen and Aggrecan of ECM (P<0.01).


CONCLUSIONS
PAP can significantly resist rat chondrocyte senescence occurred in subcultivation.
Chen
Xiao-dong
XD
Shenzhen Pingle Hospital of Orthopedics and Traumatology, Shenzhen 518010, Guangdong, China. cxdf22@163.com
Lin
Jian-hua
JH
D053674:Aggrecans / Q000235:genetics; D000818:Animals; D000999:Antlers / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics; D006025:Glycosaminoglycans / Q000032:analysis; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D053674:Aggrecans; D024043:Collagen Type II; D006025:Glycosaminoglycans; D010455:Peptides; D012333:RNA, Messenger