title: Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus.
abstract: With the widespread presence of influenza virus H5N1 in poultry and wildlife species, particularly migrating birds, vaccination has become an important control strategy for avian influenza (AI). In this study, the immune efficacy and hemagglutination inhibition (HI) antibody responses induced by a recombinant fowlpox virus (FPV) vector-based rFPV-HA-NA vaccine was evaluated in SPF and commercial chickens. Four-week old SPF chickens vaccinated with one dose of vaccine containing 2 x 10(3) plaque forming units (PFU) of virus were completely protected from H5N1 AI virus 1 week after vaccination, and protective immunity lasted for at least 40 weeks. Two-week old commercial layer chickens were vaccinated with the rFPV-HA-NA vaccine and boosted with the same dose of vaccine following an interval of 18 weeks. The HI antibody titers higher than 4log2 lasted for 52 weeks after the booster immunization. We also examined the efficacy of the rFPV-HA-NA vaccine in SPF chickens administrated by different routes. The results showed that effective application of rFPV-HA-NA vaccine in poultry may be restricted to wing-web puncture, intramuscular or subcutaneous injection. These results demonstrate that the rFPV-HA-NA vaccine is effective in the prevention of infection of H5N1 AI virus.
authors.affiliation: Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, PR China.
authors.forename: Chuanling
authors.initials: C
authors.lastname: Qiao
authors.forename: Yongping
authors.initials: Y
authors.lastname: Jiang
authors.forename: Guobin
authors.initials: G
authors.lastname: Tian
authors.forename: Xiurong
authors.initials: X
authors.lastname: Wang
authors.forename: Chengjun
authors.initials: C
authors.lastname: Li
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Xin
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
authors.forename: Kangzhen
authors.initials: K
authors.lastname: Yu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D005587:Fowlpox virus / Q000235:genetics*; D006385:Hemagglutination Inhibition Tests; D007117:Immunization, Secondary; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D016019:Survival Analysis; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines