Three distinct condensin complexes control C. elegans chromosome dynamics.
BACKGROUND
Condensin complexes organize chromosome structure and facilitate chromosome segregation. Higher eukaryotes have two complexes, condensin I and condensin II, each essential for chromosome segregation. The nematode Caenorhabditis elegans was considered an exception, because it has a mitotic condensin II complex but appeared to lack mitotic condensin I. Instead, its condensin I-like complex (here called condensin I(DC)) dampens gene expression along hermaphrodite X chromosomes during dosage compensation.


RESULTS
Here we report the discovery of a third condensin complex, condensin I, in C. elegans. We identify new condensin subunits and show that each complex has a conserved five-subunit composition. Condensin I differs from condensin I(DC) by only a single subunit. Yet condensin I binds to autosomes and X chromosomes in both sexes to promote chromosome segregation, whereas condensin I(DC) binds specifically to X chromosomes in hermaphrodites to regulate transcript levels. Both condensin I and II promote chromosome segregation, but associate with different chromosomal regions during mitosis and meiosis. Unexpectedly, condensin I also localizes to regions of cohesion between meiotic chromosomes before their segregation.


CONCLUSIONS
We demonstrate that condensin subunits in C. elegans form three complexes, one that functions in dosage compensation and two that function in mitosis and meiosis. These results highlight how the duplication and divergence of condensin subunits during evolution may facilitate their adaptation to specialized chromosomal roles and illustrate the versatility of condensins to function in both gene regulation and chromosome segregation.
Csankovszki
Gyorgyi
G
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. gyorgyi@umich.edu
Collette
Karishma
K
Spahl
Karin
K
Carey
James
J
Snyder
Martha
M
Petty
Emily
E
Patel
Uchita
U
Tabuchi
Tomoko
T
Liu
Hongbin
H
McLeod
Ian
I
Thompson
James
J
Sarkeshik
Ali
A
Sarkesik
Ali
A
Yates
John
J
Meyer
Barbara J
BJ
Hagstrom
Kirsten
K
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D048750:Cell Nucleus Division / Q000502:physiology; D020090:Chromosome Segregation / Q000502:physiology*; D002875:Chromosomes / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004303:Dosage Compensation, Genetic / Q000235:genetics* / Q000502:physiology; D019143:Evolution, Molecular; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D047468:Immunoprecipitation; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D040901:Proteomics / Q000379:methods; D034622:RNA Interference
D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C108312:condensin complexes; D000251:Adenosine Triphosphatases