title: [Effects of genistein on expression of TGF-beta1 and intracellular free Ca2+ concentration in fibroblasts derived from human hypertrophic scar].
abstract: OBJECTIVE
  To observe the effects of Genistein on TGF-beta1 expression and the intracellular free Ca2+ concentration in human hypertrophic scar fibroblasts, and to discuss the mechanism of the anti-fibrosis effect.
  
  
  METHODS
  Fibroblasts were derived from human hypertrophic scar tissue and cultured in vitro. Genistein in different concentrations (25, 50, 100 micromol/L) was administrated to the fibroblasts, respectively. After 48 hours of co-culture, the expression of TGF-beta1 mRNA and protein were examined by RT-PCR and Western-Blot assay respectively. The intracellular free Ca2+ concentration in hypertrophic scar fibroblasts pretreated by Genistein was determined by laser confocal scanning microscopy with or without the stimulation of bFGF.
  
  
  RESULTS
  Genistein inhibited the expression of TGF-beta1 in hypertrophic scar fibroblasts on a concentration-dependent manner. bFGF significantly elevated the intracellular free Ca2+ concentration, however its stimulating effect was remarkably alleviated when the fibroblasts were pre-treated by Genistein.
  
  
  CONCLUSIONS
  Genistein can reduce the expression of TGF-beta1 and block the accumulation of intracellular free calcium induced by growth factors. It maybe one of the possible mechanisms of Genistein's antifibrosis effect.
authors.affiliation: Department of Plastic Surgery, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Cao
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Dai
authors.forename: Zhi
authors.initials: Z
authors.lastname: Feng
authors.forename: Shan
authors.initials: S
authors.lastname: Xia
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Chen
authors.forename: Heng
authors.initials: H
authors.lastname: Yao
mesh_terms: D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1; D019833:Genistein; D002118:Calcium