title: Gelsolin down-regulates lipopolysaccharide-induced intraamniotic tumor necrosis factor-alpha production in the midtrimester of pregnancy.
abstract: OBJECTIVE
  The purpose of this study was to identify gelsolin in midtrimester amniotic fluid and evaluate its interaction with lipopolysaccharide (LPS).
  
  
  METHODS
  Supernatants from 40 midtrimester amniotic fluid samples were incubated with Escherichia coli LPS, and gelsolin binding was measured by enzyme-linked immunosorbent assay. Unfractionated aliquots of 25 of the fluids were cultured ex vivo for 24 hours in the presence of LPS and supernatants tested for tumor necrosis factor (TNF)-alpha and interleukin (IL)-10 production, and the influence of antigelsolin antibody was evaluated.
  
  
  RESULTS
  Each amniotic fluid was positive for gelsolin that bound to LPS. LPS-induced TNF-alpha production was inversely proportional to the amniotic fluid concentrations of LPS-bound gelsolin (r = -0.5047; P = .006). Preincubation with monoclonal antibody to gelsolin led to an increase in LPS-induced TNF-alpha production (P = .01). There was no relationship between gelsolin and IL-10 production.
  
  
  CONCLUSIONS
  Gelsolin is present in midtrimester amniotic fluid, binds to LPS, and inhibits the induction of TNF-alpha.
authors.affiliation: Division of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY 10021, USA.
authors.forename: Devrim
authors.initials: D
authors.lastname: Sezen
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: Bongiovanni
authors.forename: Shari
authors.initials: S
authors.lastname: Gelber
authors.forename: Uma
authors.initials: U
authors.lastname: Perni
authors.forename: J Milton
authors.initials: JM
authors.lastname: Hutson
authors.forename: Daniel
authors.initials: D
authors.lastname: Skupski
authors.forename: Steven S
authors.initials: SS
authors.lastname: Witkin
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D018260:Gelsolin / Q000276:immunology*; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D018260:Gelsolin; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha