title: Assessment of the extent of starch dissolution in dimethyl sulfoxide by 1H NMR spectroscopy.
abstract: Complete dissolution is needed for the separation, characterization, or homogeneous labeling of whole starch molecules. A method is presented to quantify the extent of starch dissolution in DMSO for the first time; it is validated on a commercial rice starch. It is used directly on starch dispersions containing possible undissolved or co-dissolved species. High-amylose maize starches, known to be digested slowly in vivo, only quantitatively dissolve in the presence of high concentrations of an H-bond disrupter, LiBr, although they form clear dispersions at low LiBr concentrations. Starch quantitatively dissolves from waxy rice flours; non-starch components partially co-dissolve but do not interfere with the dissolution quantification.
authors.affiliation: The University of Queensland, Centre for Nutrition and Food Sciences, Brisbane, QLD, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schmitz
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Dona
authors.forename: Patrice
authors.initials: P
authors.lastname: Castignolles
authors.forename: Robert G
authors.initials: RG
authors.lastname: Gilbert
authors.forename: Marianne
authors.initials: M
authors.lastname: Gaborieau
mesh_terms: D001965:Bromides / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D006801:Humans; D018020:Lithium Compounds / Q000737:chemistry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012275:Oryza / Q000737:chemistry; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry
chemical_list: D001965:Bromides; D018020:Lithium Compounds; D012997:Solvents; C040949:lithium bromide; D013213:Starch; D004121:Dimethyl Sulfoxide