title: Embryonic expression of Drosophila IMP in the developing CNS and PNS.
abstract: Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nervous system, and IMP up-regulation in solid tumors correlates with a high metastatic potential and poor prognosis. In this study, we used immunohistochemistry and live-imaging of an endogenous promoter-driven GFP-dIMP fusion protein to reveal the expression pattern of dIMP protein throughout embryogenesis. In the cellular blastoderm, immunoreactivity was seen in the entire cell-layer, where it was localized apically to the nucleus, and in the pole cells. Later, the GFP-dIMP fusion protein appeared in the developing central nervous system, both in the brain and in the ventral nerve cord. In the peripheral nervous system, immunoreactivity was detected in both neurons and accessory cells of chordotonal and external sensory organs.
authors.affiliation: Department of Biology, University of Copenhagen, Ole Maaloes Vej 5, DK-2200 Copenhagen N, Denmark. sidsela@bio.ku.dk
authors.forename: Sidsel Kramshøj
authors.initials: SK
authors.lastname: Adolph
authors.forename: Robert
authors.initials: R
authors.lastname: DeLotto
authors.forename: Finn Cilius
authors.initials: FC
authors.lastname: Nielsen
authors.forename: Jan
authors.initials: J
authors.lastname: Christiansen
mesh_terms: D000818:Animals; D001756:Blastoderm / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000378:metabolism; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004817:Epidermis / Q000196:embryology / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins; D011984:Sensory Receptor Cells / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; C498778:Imp protein, Drosophila; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins