Corticotropin-releasing hormone receptor subtypes in the rat anterior pituitary after two types of restraint stress.
The stress response in anterior pituitary (AP) is mediated by corticotropin-releasing hormone (CRH) acting through CRH-R1 and -R2, however, the function of CRH-R2 in AP is still not fully elucidated. We used 1-h long restraint (IMO) as well as restraint combined with water immersion (IMO+C). Using real-time PCR we quantified mRNA expression of CRH-R1, CRH-R2alpha-soluble and -insoluble, and cAMP response element binding (CREB), with reference gene GAPDH. In control AP, CRH-R1 mRNA was up to 20-fold higher than levels of CRH-R2alpha-soluble or CRH-R2alpha-insoluble mRNA. IMO reduced CRH-R1 mRNA to 47% and 63% of control levels 1 and 2 h after the onset of stressor, respectively, while IMO+C did not produce significant changes. Our data demonstrated that these stressors did not change CRH-R2alpha mRNA, unlike the very significant response of CRH-R1 to IMO.
Klenerova
Vera
V
Laboratory of Biochemical Neuropharmacology, Institute of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. vera.klenerova@LF1.cuni.cz
Sery
Omar
O
Hynie
Sixtus
S
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D007101:Immersion; D008297:Male; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D012149:Restraint, Physical; D013315:Stress, Psychological; D000099052:CRF Receptor, Type 1
C101531:CRF receptor type 2; D017362:Cyclic AMP Response Element-Binding Protein; D020033:Protein Isoforms; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D000099052:CRF Receptor, Type 1; D003346:Corticotropin-Releasing Hormone