title: Glycoproteomic analysis of plasma from patients with atopic dermatitis: CD5L and ApoE as potential biomarkers.
abstract: Atopic dermatitis (AD) is an inflammatory skin disorder that is both uncomfortable and distressing to patients, and its prevalence has been steadily increasing. It is obvious that the identification of efficient markers of AD in plasma would offer the possibility of effective diagnosis, prevention, and treatment strategies. In this study, a proteomic approach was used to analyze plasma glycoproteins from both children with AD and healthy child donors. Several protein spots showing significant quantitative changes in the AD patients were identified. Through sequential studies, it was confirmed that CD5L and ApoE were significantly up-regulated or down-regulated, respectively, in the plasma from AD patients compared with that from healthy donors. In addition, we suggest that the up-regulated CD5L in AD patients causes eosinophilia by inhibiting apoptosis or promoting the proliferation of eosinophils either in combination with or without IL-5. The glycoproteomic data in this study provides clues to understanding the mechanism of atopic alterations in plasma and suggests AD-related proteins can be used as candidate markers for AD.
authors.affiliation: Translational Research Center, KRIBB, Daejeon 305-806, Korea.
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
authors.forename: Hyang Ran
authors.initials: HR
authors.lastname: Hwang
authors.forename: Do Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Phil Young
authors.initials: PY
authors.lastname: Lee
authors.forename: Yu Jung
authors.initials: YJ
authors.lastname: In
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Ryu
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
authors.forename: Kwang Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
mesh_terms: D001057:Apolipoproteins E / Q000097:blood*; D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D049109:Cell Proliferation; D002648:Child; D003876:Dermatitis, Atopic / Q000378:metabolism*; D004802:Eosinophilia / Q000378:metabolism; D004804:Eosinophils / Q000502:physiology; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D015848:Interleukin-5 / Q000378:metabolism; D008297:Male; D040901:Proteomics; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B / Q000097:blood*
chemical_list: D001057:Apolipoproteins E; D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; C492821:CD5L protein, human; D006023:Glycoproteins; D015848:Interleukin-5; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B