title: In vitro study of matrix surface properties of porous granulated calcium phosphate ceramic materials made in Russia.
abstract: We performed in vitro screening of monophasic (hydroxyapatite, beta-tricalcium phosphate, carbonate-substituted hydroxyapatite with 0.59 and 5.9 wt% substitution with CO(3)(2-)) and biphasic (hydroxyapatite-tricalcium phosphate with various percentage of the components 80/20, 60/40, 20/80, silicon-substituted hydroxyapatite with 0.79 wt% SiO(2)) porous granulated ceramics composed of calcium phosphate powders synthesized by methods of heterophasic interaction of reagents and precipitation from aqueous solutions using MTT test and cultured human fibroblasts. Acute toxicity of materials (24-h incubation with cell culture) and matrix properties (3, 5, 7, 14, 18, 21, 28 days in culture) were evaluated. We selected a batch of materials obtained by precipitation from aqueous solutions, which were non-toxic and were characterized by good matrix properties (for cells). Biphasic ceramics with hydroxyapatite-tricalcium phosphate ratio of 80/20 exhibited best characteristics, and ceramics on the basis of silicon-substituted hydroxyapatite showed moderate characteristics.
authors.affiliation: P. A. Gertsen Moscow Research Oncological Institute, Federal Agency for High-Technological Medical Care, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Chissov
authors.forename: I K
authors.initials: IK
authors.lastname: Sviridova
authors.forename: N S
authors.initials: NS
authors.lastname: Sergeeva
authors.forename: V A
authors.initials: VA
authors.lastname: Kirsanova
authors.forename: S A
authors.initials: SA
authors.lastname: Achmedova
authors.forename: M M
authors.initials: MM
authors.lastname: Filiushin
authors.forename: S M
authors.initials: SM
authors.lastname: Barinov
authors.forename: I V
authors.initials: IV
authors.lastname: Fadeeva
authors.forename: V S
authors.initials: VS
authors.lastname: Komlev
authors.forename: V V
authors.initials: VV
authors.lastname: Smirnov
mesh_terms: D001672:Biocompatible Materials / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006882:Hydroxyapatites / Q000032:analysis / Q000737:chemistry; D008422:Materials Testing; D016062:Porosity; D011208:Powders; D012426:Russia; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; D011208:Powders; C018392:tricalcium phosphate