Eliminating heterophilic antibody interference for ferritin detection using Olympus F(ab')2 based reagent.
Interferences with the Olympus immunoturbidimetric assay for ferritin have been reported because the antibodies used in the immunoassay are derived from rabbits. Rabbits are familiar pets known to be a risk factor for developing heterophilic (or interfering) antibodies. This report shows how the current Olympus Ferritin assay has been improved to eliminate the interference from heterophilic antibodies.
Grouzmann
Eric
E
Laboratoires Meditest SA, 1800, Vevey, Switzerland. eric.grouzmann@meditest.ch
Dayer
Nicole
N
Bain
Catherine
C
Neville
Claire M
CM
McCusker
Matthew D
MD
Wang
Robert Y
RY
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D000918:Antibody Specificity; D005189:False Positive Reactions; D005293:Ferritins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D011817:Rabbits; D012680:Sensitivity and Specificity
D000910:Antibodies, Heterophile; D007140:Immunoglobulin Fab Fragments; D005293:Ferritins