[Experimental study on the treatment of serious soft tissue injuries with strengthening the spleen and replenishing qi].
OBJECTIVE
To study the effects of Chinese drugs based on strengthening the spleen and replenishing qi treatment rule on neoformative capillaries and fibroblast during the soft tissue repair after serious trauma in rats, so as to explore the biological basis of the TCM theory "the spleen dominate extremities and muscles" applied to the treatment of soft tissue injuries.


METHODS
The model rats were established by bleeding from femoral artery and lancing method, and the rats were randomly divided into the control group, strengthening the spleen group and activating blood and resolving stasis group. The samples were got from the tissue of the wounded area at the 5th, 10th and 15th days after oral administration of the traditional Chinese medicine. After fixation and section, the tissues were stained by CD31 and PCNA staining. The amount of the capillaries and fibroblasts in the tissue of the wounded area were observed through multi-purpose microscope (ZEISS Axioskop2). Quantitative analysis was carried out on Image-ProPlus image analyzer.


RESULTS
The amount of the capillaries and fibroblasts in the wounded tissue in the strengthening the spleen group were larger than that in the control group at the 5th, 10th and 15th day. And the proliferation speed of capillaries and fibroblasts was faster than those in the control group or the activating blood and resolving stasis group.


CONCLUSIONS
The Chinese drugs according to strengthening the spleen and replenishing qi treatment rule were effective to promote growth of the granulation tissue and facilitate healing of the wounded area. And it has better effect than the treatment of promoting blood circulation and removing stasis.
Chen
Xun-wen
XW
Department of Orthopaedics, the Foshan Hospital Affiliated to Guangzhou University of TCM, Foshan 528000, Guangdong, China.
Zhu
Yong-zhan
YZ
Chen
Zhi-wei
ZW
Wu
Zheng-jie
ZJ
He
Li-lei
LL
D000818:Animals; D002196:Capillaries / Q000473:pathology; D002452:Cell Count; D049109:Cell Proliferation; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D008297:Male; D019413:Qi; D051381:Rats; D017695:Soft Tissue Injuries / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D013154:Spleen / Q000502:physiology*; D014945:Wound Healing