title: Suppression of protein adsorption on a charged phospholipid polymer interface.
abstract: High capability of a charged interface to suppress adsorption of both anionic and cationic proteins was reported. The interface was covalently constructed on quartz by modifying with an anionic phospholipid copolymer, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-potassium 3-methacryloyloxypropyl sulfonate (PMPS)-co-3-methacryloxypropyl trimethoxysilane (MPTMSi)) (PMBSSi). The PMBSSi interfaces were very hydrophilic and homogeneous and could function effectively for a long time even under long-term fluidic working conditions. The PMBSSi density on the interface, which was controllable by adjusting the PMBSSi concentration of the modification solution, affected the surface properties, including the surface contact angle, the surface roughness, and the surface zeta-potential. When a PMBSSi modification was applied, the adsorption of various proteins (isoelectric point varying from 1.0 to 11.0) on quartz was reduced to at least 87% in amount, despite the various electrical natures these proteins have. The protein adsorption behavior on the PMBSSi interface depended more on the PMBSSi density than on the surface charge. The PMBSSi modification had a stable impact on the surface, not only at the physiologic ionic strength, but also over a range of the ionic strength, suggesting that electrostatic interactions do not dominate the behavior of protein adsorption to the PMBSSi surface.
authors.affiliation: Department of Materials Engineering, School of Engineering, and Center for NanoBio Integration, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. xuyan@icl.t.u-tokyo.ac.jp
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Madoka
authors.initials: M
authors.lastname: Takai
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
mesh_terms: D000327:Adsorption; D010743:Phospholipids; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011791:Quartz; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D010743:Phospholipids; D011108:Polymers; D011506:Proteins; D011791:Quartz