title: A fluorescent probe for diacetyl detection.
abstract: A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.
authors.affiliation: Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Axel
authors.initials: A
authors.lastname: Duerkop
authors.forename: Otto S
authors.initials: OS
authors.lastname: Wolfbeis
mesh_terms: D003931:Diacetyl / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D015394:Molecular Structure; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; D012235:Rhodamines; C543681:rhodamine B hydrazide; D003931:Diacetyl