Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation.
GSK-3 activity mediates cAMP repression of stalk induction of cells in low-density monolayer culture. The lower the GSK-3 activity the greater the percentage of stalk cells formed. This protocol describes a robust and quantitative method utilizing an adapted stalk cell monolayer assay to measure GSK-3 activation.
Harwood
Adrian J
AJ
Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D000242:Cyclic AMP / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology / Q000201:enzymology; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta
D000242:Cyclic AMP; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3