A new method for the determination of nucleic acid using an Eu3+- nicotinic acid complex as a resonance light scattering probe.
This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu(3+)-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7 x 10(-8)-1 x 10(-5 ) g x mL(-1) for fsDNA, and its detection limit is 2 x 10(-8 ) g x mL(-1). Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism is also studied. It is thought that nucleic acid can bind with the Eu(3+)-nicotinic acid complex through electrostatic attraction and thus form a large Eu(3+)-nicotinic acid-nucleic acid complex.
Guo
Meng
M
Microscale Science Institute, Department of Chemistry, Weifang University, Weifang 261061, PR China. guomeng7101@126.com
Wang
Lin-Tong
LT
Wu
Xia
X
Xu
Wei
W
Yang
Jing-He
JH
D004247:DNA / Q000032:analysis*; D005063:Europium / Q000737:chemistry*; D008027:Light; D008722:Methods; D009525:Niacin / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D012542:Scattering, Radiation; D014325:Tromethamine
D014325:Tromethamine; D009525:Niacin; D005063:Europium; D012313:RNA; D004247:DNA