Transforming growth factor-beta1 shows an incremental osteoinductive dose-response relationship.
OBJECTIVE
To determine if there is a dose-response curve for TGF-beta1 in a rabbit calvarial defect model.


METHODS
Controlled animal study using arms of increasing concentrations of TGF-beta1 to evaluate the osteoinductive potential of each arm.


METHODS
Sixteen skeletally mature New Zealand white rabbits were randomized into control and experimental arms. Incremental doses of TGF-beta1 delivered in an inactivated guanidine-extracted demineralized bone matrix (Gu-DBM) carrier were implanted into a critically sized calvarial defect. The animals were sacrificed at 4 weeks and histomorphometric analysis was then accomplished.


RESULTS
TGF-beta1 showed a dose-response relationship, with the higher doses chosen for this study causing more robust osteoinduction.


CONCLUSIONS
In this pilot dosing study, TGF-beta1 demonstrates increasing osteoinduction with increasing dose levels in this animal model.
Moxham
J Paul
JP
Department of Surgery, University of British Columbia, Vancouver, Canada. pmoxham@cw.bc.ca
Wong
Kevin K
KK
Kibblewhite
Douglas J
DJ
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D010865:Pilot Projects; D011817:Rabbits; D011897:Random Allocation; D012886:Skull / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000008:administration & dosage* / Q000494:pharmacology*
D053773:Transforming Growth Factor beta1