title: A cost-effective device for the rapid transfer of gel-separated proteins onto membranes.
abstract: We describe here the fabrication of a cost-effective semi-dry blotting apparatus for the transfer of proteins onto membranes. Graphite sheets were used as electrodes. Protein mixtures were separated on NuPAGE 4% to 12% polyacrylamide gradient gels. With a Tris-bicine buffer, we demonstrated that close to 80% of the proteins with apparent molecular mass of 80kDa or less were removed from the gels after 8min of blotting. The process is much faster than the techniques reported previously in the literature.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, NanKang Taipei, Taiwan, ROC.
authors.forename: Hann W
authors.initials: HW
authors.lastname: Tam
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Huang
authors.forename: Ming F
authors.initials: MF
authors.lastname: Tam
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D003362:Cost-Benefit Analysis; D004566:Electrodes; D004591:Electrophoresis, Polyacrylamide Gel; D008567:Membranes, Artificial; D008722:Methods; D011506:Proteins / Q000302:isolation & purification*; D012107:Research Design; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D011506:Proteins