Polyelectrolyte coated PLGA nanoparticles: templation and release behavior.
Poly[(D,L-lactide)-co-glycolide] nanoparticles coated with polyethyleneimine on their surface were prepared by an emulsification-solvent evaporation method and subsequently surface modified by LBL assembly. The assembly of poly(acrylic acid) and polyethyleneimine on a planar substrate and on the PLGA nanoparticles was monitored by QCM-D, zeta-potential, flow cytometry and TEM. Carboxylic and amino groups in the multilayers were crosslinked by carbodiimide condensation, which was also later used to graft poly(ethylene glycol) (PEG). Rhodamine 6G, 5(6)-carboxyfluorescein and fluorescein were incorporated into the nanoparticles and their release profiles were recorded at 60 degrees C and at 37 degrees C for rhodamine 6G, for nanoparticles with a multilayer coating, and those that were crosslinked and grafted with PEG.
Zhou
Jie
J
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
Moya
Sergio
S
Ma
Lie
L
Gao
Changyou
C
Shen
Jiacong
J
D005434:Flow Cytometry; D019793:Fluorescein; D005452:Fluoresceins; D019344:Lactic Acid / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011092:Polyethylene Glycols; D011094:Polyethyleneimine / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012235:Rhodamines
D005452:Fluoresceins; D012235:Rhodamines; C026188:rhodamine 6G; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; C024098:6-carboxyfluorescein; D019344:Lactic Acid; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D019793:Fluorescein