[Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain].
OBJECTIVE
To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain.


METHODS
Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.


RESULTS
The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.


CONCLUSIONS
Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.
Huang
Ying
Y
Agriculture Department of Jilin University, Changchun 130062, China.
Hu
Rong-Liang
RL
Tang
Qing
Q
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D011818:Rabies / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins