title: Polyvalent DNA nanoparticle conjugates stabilize nucleic acids.
abstract: Polyvalent oligonucleotide gold nanoparticle conjugates have unique fundamental properties including distance-dependent plasmon coupling, enhanced binding affinity, and the ability to enter cells and resist enzymatic degradation. Stability in the presence of enzymes is a key consideration for therapeutic uses; however the manner and mechanism by which such nanoparticles are able to resist enzymatic degradation is unknown. Here, we quantify the enhanced stability of polyvalent gold oligonucleotide nanoparticle conjugates with respect to enzyme-catalyzed hydrolysis of DNA and present evidence that the negatively charged surfaces of the nanoparticles and resultant high local salt concentrations are responsible for enhanced stability.
authors.affiliation: Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
authors.forename: Dwight S
authors.initials: DS
authors.lastname: Seferos
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Prigodich
authors.forename: David A
authors.initials: DA
authors.lastname: Giljohann
authors.forename: Pinal C
authors.initials: PC
authors.lastname: Patel
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*
chemical_list: D004247:DNA