A novel chromogenic medium for isolation of Pseudomonas aeruginosa from the sputa of cystic fibrosis patients.
BACKGROUND
A novel chromogenic medium for isolation and identification of Pseudomonas aeruginosa from sputa of cystic fibrosis (CF) patients was evaluated and compared with standard laboratory methods.


METHODS
One hundred sputum samples from distinct CF patients were cultured onto blood agar (BA), Pseudomonas CN selective agar (CN) and a Pseudomonas chromogenic medium (PS-ID). All Gram-negative morphological variants from each medium were subjected to antimicrobial susceptibility testing, and identification using a combination of biochemical and molecular methods.


RESULTS
P. aeruginosa was isolated from 62 samples after 72 h incubation. Blood agar recovered P. aeruginosa from 56 samples (90.3%) compared with 59 samples (95.2%) using either CN or PS-ID. The positive predictive value of PS-ID (98.3%) was significantly higher than growth on CN (88.5%) for identification of P. aeruginosa (P<0.05).


CONCLUSIONS
PS-ID is a promising medium allowing for the isolation and simultaneous identification of P. aeruginosa from sputa of CF patients.
Laine
Larissa
L
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, UK.
Perry
John D
JD
Lee
Jenner
J
Oliver
Michelle
M
James
Arthur L
AL
De La Foata
Corinne
C
Halimi
Diane
D
Orenga
Sylvain
S
Galloway
Angela
A
Gould
F Kate
FK
D000293:Adolescent; D000328:Adult; D000362:Agar; D001431:Bacteriological Techniques; D002648:Child; D002675:Child, Preschool; D002863:Chromogenic Compounds; D003470:Culture Media; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D011237:Predictive Value of Tests; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology*; D055815:Young Adult
D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar