Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain.
In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections.
Moretta
Rosalía
R
CICVyA-INTA Castelar, Buenos Aires, Argentina.
Ruybal
Paula
P
Mesplet
María
M
Petrigh
Romina
R
Nuñez
Pablo
P
Gil
Gonzalo
G
Wilkowsky
Silvina
S
Garbossa
Graciela
G
Farber
Marisa
M
D042322:Anaplasma marginale / Q000302:isolation & purification*; D000818:Animals; D004912:Erythrocytes / Q000382:microbiology; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015203:Reproducibility of Results; D013194:Staining and Labeling
D005456:Fluorescent Dyes