[Experimental study on the effect of sensitized sera on engraftment of hematopoietic stem cells].
OBJECTIVE
To evaluate the effect of sensitized sera on engraftment of hematopoietic stem cells (HSCs) and its mechanism.


METHODS
Bone marrow cells (BMCs) from C57BL/6 mice were incubated with sensitized sera (group I) or normal sera (group II), and then washed and transplanted into irradiated BALB/c mice. The survival analysis and engraftment evaluation of the recipients were observed. The incubated BMCs were bound with goat anti mouse IgG for and labeled with Annexin V for apoptosis detection.


RESULTS
Seven out of ten recipient mice in group I died of bone marrow failure at day 10 after transplantation, while all of those in group II were long-term survived. Engraftment assay showed recipients blood count and BMCs progressively decreased along with time passing in group I; in addition, the chimeric percentage of donor cells progressively decreased as well. The percentage of BMCs binding with goat anti mouse IgG in group I or group II were (90.3 +/- 5.1)% and (5.2 +/- 2.4)%, respectively, and the difference was statistically significant (P < 0.01). However, no significant difference was found in the apoptosis detection between the two groups (P > 0.05).


CONCLUSIONS
The engraftment capacity of HSCs is significantly impaired by sensitized sera, the antibodies in sensitized sera may bind to antigens expressed on HSCs but do not induce apoptosis.
Xu
Lu-Hong
LH
Hematopoietic Stem Cell Transplantation Center, Department of Pediatrics, The Second Affiliated Hospital, SUN Yat-sen University, Guangzhou 510120, China.
Fang
Jian-Pei
JP
Xu
Hong-Gui
HG
Weng
Wen-Jun
WJ
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D018380:Hematopoietic Stem Cell Transplantation; D007106:Immune Sera; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
D017304:Annexin A5; D007106:Immune Sera