[Study on small diameter artificial vascular graft with eNOS gene transfected endothelial cells in canine].
OBJECTIVE
To investigate the patency efficacy of small diameter artificial vascular graft with eNOS gene transfected endothelial cells in canine.


METHODS
By using the two steps high pressure injection, eNOS gene transfected endothelial cells were planted on artificial vascular graft with a diameter of 3 mm. The artificial vascular grafts were transplanted into canine femoral artery, and the patency of the artery was observed through digital subtracted angiography (DSA) and electron telescope 1, 4, 12 and 24 weeks after the operation.


RESULTS
The adherence rate of eNOS gene transfected endothelial cells to artificial vascular grafts was up to 90%. For 10 days, the cells extended and formed a continuous intima. One week after the operation, 3/9 grafts were obstructed in the group of simple artificial vascular grafts; 4 weeks after, all of grafts (9/9) were obstructed in the group of simple artificial vascular grafts and almost half grafts (5/10, 4/9) in un-transfected groups were obstructed; 12 weeks after, all of the grafts were obstructed in the group of simple artificial vascular grafts and in un-transfected groups (9/9, 9/10); 24 weeks after, all of the grafts were obstructed in the groups of un-transfected artificial vascular grafts, while nearly all of grafts (8/10) were open in eNOS groups. Electron microscope scanning showed that endothelial cells of artificial vascular grafts in eNOS transfected groups arranged closely and formed a continuous intima; only few red blood cells, leucocytes, platelets deposited at the surface of endothelial cells in the artificial vascular grafts.


CONCLUSIONS
The patency efficacy of eNOS gene transfected endothelial cells planting on artificial vascular graft is satisfactory. It could provide an experimental basis for further clinical application of the artificial vascular grafts.
Xue
Guan-hua
GH
Department of Vascular Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China. guanhuaxue@yeah.net
Zhang
Ji-wei
JW
Zhang
Hao
H
Zhang
Bai-gen
BG
D000818:Animals; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D002478:Cells, Cultured; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics*; D011897:Random Allocation; D014162:Transfection
D019001:Nitric Oxide Synthase