title: Field collection and genetic classification of tick-borne Rickettsiae and Rickettsiae-like pathogens from South Texas: Coxiella burnetii isolated from field-collected Amblyomma cajennense.
abstract: We are reporting the first known isolation of the Q-fever agent Coxiella burnetii from field-collected cayenne ticks Amblyomma cajennense in North America. Q-fever affects a number of domestic ungulates where it can lead to abortion in sheep and goats. There is far less known about the disease's effects on wild species, primarily because of the tendency of the disease to self resolve and to provide long-term immunity to subsequent infections. The first recovery of C. burnetii in North America was from the tick species Dermacentor andersoni. Since the original isolation C. burnetii has been recovered from five other North American tick species. The currently accepted mode for the majority of human infections is inhalation. The Centers for Disease Control and Prevention, Division of Viral and Rickettsial Diseases, Rickettsial Zoonoses Branch asserts the Q-fever agent as requiring as few as one organism to cause disease via inhalation in susceptible humans. However, with more and more isolations from ticks, evidence linking C. burnetii and ticks is mounting. The true role of tick species as competent vectors is still unconfirmed. Preemptive field collections of possible vector arthropods, hosts, and reservoirs can provide invaluable baseline environmental data that will prove supportive in follow-up studies and abatement efforts.
authors.affiliation: Air Force Institute of Technology - Civilian Institute Program, Texas A&M University, Department of Entomology, College Station, Texas, USA. dsander8@gimail.af.mil
authors.forename: David M
authors.initials: DM
authors.lastname: Sanders
authors.forename: Jill E
authors.initials: JE
authors.lastname: Parker
authors.forename: Wes W
authors.initials: WW
authors.lastname: Walker
authors.forename: Matt W
authors.initials: MW
authors.lastname: Buchholz
authors.forename: Keith
authors.initials: K
authors.lastname: Blount
authors.forename: Johnathan L
authors.initials: JL
authors.lastname: Kiel
mesh_terms: D000818:Animals; D001483:Base Sequence; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D013781:Texas; D013987:Ticks / Q000382:microbiology*
chemical_list: D017931:DNA Primers