Lysophosphatidylcholine induces endothelial cell injury by nitric oxide production through oxidative stress.
OBJECTIVE
To determine whether lysophosphatidylcholine (LPC) induces endothelial cell injury by altering the production of nitric oxide (NO) and thereby increasing reactive oxygen species (ROS).


METHODS
Human umbilical vein endothelial cells (HUVECs) were cultured and exposed to LPC, LPC with N(G)-nitro-l-arginine methyl ester (L-NAME), LPC with antioxidants. LPC-induced cell injury and viability were determined using LDH and Resazurin assays. The Mann-Whitney U test was used for statistical analysis.


RESULTS
LPC induced HUVEC injury in a concentration-dependent manner. LPC induced the overproduction of NO and ROS in HUVECs and LPC-induced HUVEC injury is significantly inhibited by the eNOS inhibitor (L-NAME) and the antioxidants (p < 0.05).


CONCLUSIONS
These findings suggest that LPC induces the overproduction of NO, which may increase the oxidative stress on endothelial cells and lead to endothelial cell injury.
Kim
Eon A
EA
Department of Obstetrics and Gynecology, Ewha Womans University, Seoul 158-051, Korea.
Kim
Ji Ae
JA
Park
Mi Hye
MH
Jung
Sung Chul
SC
Suh
Suk Hyo
SH
Pang
Myung-Geol
MG
Kim
Young Ju
YJ
D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology
D000975:Antioxidants; D005452:Fluoresceins; D008244:Lysophosphatidylcholines; D009566:Nitrates; D009573:Nitrites; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester