Faster and stronger vascular "Biotube" graft fabrication in vivo using a novel nicotine-containing mold.
To accelerate the fabrication of in vivo-tissue engineered autologous vascular prosthetic tissues, the "Biotube," a novel drug-coating mold was designed. The mold was prepared by impregnating nicotine as a model drug into a gelatinous matrix coated on acrylate rods (diameter, 2 mm; length, 20 mm). Upon embedding the molds into dorsal subcutaneous pouches of rats, completely encapsulated Biotubes with significant tissue migration accompanied by rich angiogenesis and having 3.8 times as many neovessels as the uncoated controls, were formed at only 2 weeks. The wall thickness and burst strength of the Biotubes were 399.9 +/- 135.2 microm and 2682.6 +/- 722.6 mmHg, respectively. These values were, respectively, more than 9.6 and 3.2 times greater than the corresponding controls. Therefore, it is confidently expected that the mechanical properties of Biotubes obtained by nicotine coating make them suitable for application as vascular grafts.
Sakai
Osamu
O
Department of Bioengineering, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Kanda
Keiichi
K
Takamizawa
Keiichi
K
Sato
Takayuki
T
Yaku
Hitoshi
H
Nakayama
Yasuhide
Y
D000818:Animals; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D018919:Neovascularization, Physiologic; D009538:Nicotine / Q000032:analysis*; D051381:Rats; D023822:Tissue Engineering
D001672:Biocompatible Materials; D009538:Nicotine