[Cardiac field potentials and activation sequence in Langendorff perfused heart, cardiac tissue slices and cultured ventricular myocytes recorded by microelectrode arrays system.].
OBJECTIVE
To observe field potentials (FPs) and activation sequence at Langendorff perfused guinea pigs heart, SD rat cardiac tissue strips perfused by Tyrode's solution and cultured ventricular cardiomyocytes of suckling mice by microeletrode arrays (MEA) technique.


METHODS
FPs and activation sequence were recorded from perfused heart, cardiac tissue strips (5 mm x 5 mm) and cultured ventricular cardiomyocytes by MEA.


RESULTS
(1) FPs could be recorded in hearts perfused for 30 to 90 min with a heart rate 90 - 120 beats/min. FP durations of both ventricular and atrial tissue were (210 +/- 78) ms and (164 +/- 58) ms, respectively and atrial ventricular conduction time was (320 +/- 150) ms. (2) The excitability of Tyrode's solution perfused tissue strips was visible for more than 2 h, and FP durations of ventricular and atrial tissue strips were (115.80 +/- 11.61) ms and (83.71 +/- 6.48) ms, respectively. (3) Spontaneous beating frequency (150 +/- 100) beats/min and FPs could be readily recorded in cardiomyocytes cultured between 2 to 72 hours.


CONCLUSIONS
MEAs is a sensitive, low noise and stable technique for observing local tissue action potential and activation sequence of whole heart, cardiac tissue strips and cultured cardiomyocytes.
Hou
Yue-Mei
YM
Cardiovascular Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumuqi 830054, China.
Na
Ji-Na
JN
Rayile
Aisa
A
D000200:Action Potentials; D000818:Animals; D006321:Heart; D000067268:Isolated Heart Preparation; D008839:Microelectrodes; D032383:Myocytes, Cardiac; D017207:Rats, Sprague-Dawley