Molecular profiling of T-helper immune genes during dengue virus infection.
In this study, we provide a comprehensive molecular profiling of the involvement of T- helper (Th) genes during dengue virus infection of different cell types. The Th gene profiles of three human cell types (monocytes, T-cells and hepatocytes) were analyzed simultaneously via array-based RT-PCR upon infection with dengue virus. Differential regulation of 41 Th genes was identified and of which 20 of those genes may contribute to immuno-pathogenesis of dengue virus infection by regulating inflammation, thrombocytopenia and vascular permeability. Among the strongly up-regulated genes were the RANTES, CC-CKR3, IRF4, CLEC2C, IL-6 and TLR6, which are potent inducer of inflammation and vascular permeability. Profiling genes obtained from this study may serve as potential biomarkers and the modulation of Th immune responses during dengue virus infection has important implications in disease outcome.
Chen
Jincheng
J
Department of Microbiology, Yong Loo Lin School of Medicine, 5 Science Drive 2, National University of Singapore, 117597 Singapore. g0800060@nus.edu.sg
Ng
Mary Mah Lee
MM
Chu
Justin Jang Hann
JJ
D015703:Antigens, CD / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D015415:Biomarkers; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000235:genetics; D003715:Dengue / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology; D006801:Humans; D050835:Interferon Regulatory Factors / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000235:genetics; D009000:Monocytes / Q000378:metabolism / Q000821:virology; D054397:Receptors, CCR3 / Q000235:genetics; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D051216:Toll-Like Receptor 6 / Q000235:genetics; D015854:Up-Regulation; D000099192:CD69 Antigens; D000099218:Interferon Regulatory Factor-4
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015415:Biomarkers; D018946:Chemokine CCL5; D050835:Interferon Regulatory Factors; D015850:Interleukin-6; D037181:Lectins, C-Type; D054397:Receptors, CCR3; D051216:Toll-Like Receptor 6; D000099192:CD69 Antigens; D000099218:Interferon Regulatory Factor-4