Physical properties of lipid bilayers from EPR spin labeling and their influence on chemical reactions in a membrane environment.
The influence of a variety of microenvironmental factors on the inherent reactivity of membrane-located reagents is poorly understood. A goal of this review is to provide detailed profiles of membrane properties, including hydrophobicity, oxygen and nitric oxide solubility and diffusion rates, bilayer penetration of metal ions and metal-ion complexes, and membrane order and fluidity, that can be obtained with EPR spin-labeling methods. These properties can drastically vary with membrane composition, membrane depth, and membrane domain formation, influencing the fate of chemical reactions that occur in a lipid bilayer environment.
Subczynski
Witold K
WK
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA. subczyn@mcw.edu
Widomska
Justyna
J
Feix
Jimmy B
JB
D000818:Animals; D055598:Chemical Phenomena; D002784:Cholesterol / Q000032:analysis; D004058:Diffusion; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008566:Membranes / Q000737:chemistry* / Q000378:metabolism; D008670:Metals / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D017382:Reactive Oxygen Species; D012995:Solubility
D008051:Lipid Bilayers; D008670:Metals; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D002784:Cholesterol; D010100:Oxygen