[Study on apoptosis of BEL-7402 cells induced by galangin].
OBJECTIVE
To investigate apoptosis of BEL-7402 cells induced by Galangin.


METHODS
Cytotoxicity and cell viability were assayed by MT methods. Morphologic assessment of apoptosis was performed by fluorescence microscope, cell cycle and mitochondrial membrane potential were analyzed by flow cytometry, Caspases Activities were detected by chromogenic substrate assay.


RESULTS
BEL-7402 cells treated with Galangin showed apparently cytotoxicity, IC50 of Galangin was 30.15 mg/L The growth curve showed that the ratio of growth decreased with the increasing of concentration of Galangin. The apoptosis appeared 24 hours after treated with 20-80 mg/L Galangin, morphologic changes included nuclear chromatin condensation and fluorescence strength was observed with fluorescence microscope. The activity of Caspase-9 attained to peak value at 6 h, while Caspase-6 activity was up to peak value at 12h and Caspase-3 activity attained to peak value at 18 h.


CONCLUSIONS
Galangin may induce BEL-7402 cells apoptosis by mitochondrial pathway.
Luo
Hui
H
Department of Chemistry, Guangdong Medical College, Zhanjiang, China. luohui@gdmc.edu.cn
Ma
Chao
C
Wang
Ya-jun
YJ
Chen
Jie
J
Liu
Jiang-qin
JQ
Zhang
Hai-tao
HT
D032429:Alpinia / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry
D000970:Antineoplastic Agents; D005419:Flavonoids; C037032:galangin; D020169:Caspases