Chosen IL-17 family proteins in neutrophils of patients with oral inflammation.
OBJECTIVE
Recent studies have indicated that human neutrophils (PMN), belonging to the first line of cellular defence against various infectious agents and inflammation can produce cytokines of the interleukin 17 (IL-17) family. The relation between pro-inflammatory IL-17A and anti-inflammatory IL-17E may be responsible for the development and maintenance of the inflammatory process. The study objective was to determine the expression of IL-17E and IL-17BR in confrontation with IL-17A and IL-17R in neutrophils of patients with oral cavity inflammation and to compare protein expressions in autologous peripheral blood mononuclear cells (PBMC).


METHODS
Expressions of IL-17E, IL-17A, IL-17BR and IL-17R was assessed by the Western blot method. IL-17E and IL-17A levels was determined by the ELISA method.


RESULTS
The expressions of IL-17E, IL-17A, IL-17BR and IL-17R in PMN and PBMC in patients were higher in the cells of patients, as compared to the controls. The expressions of IL-17E and IL-17BR in both kinds of cells were lower than the levels of IL-17A and IL-17R. The levels of IL-17E and IL-17A were increased in cell supernatants and blood serum of patients, as compared to the control group.


CONCLUSIONS
The preliminary examinations indicate the predominance of pro-inflammatory effects of IL-17A controlled by PMN, as well as PBMC in patients with oral inflammation. Results obtained also suggest a more significant involvement of PMN in the IL-17E and IL-17A dependent reactions.
Garley
M
M
Department of Immunology, Medical University of Bialystok, Bialystok, Poland. marzenagarley@wp.pl
Jablonska
E
E
D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation; D020381:Interleukin-17 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000378:metabolism* / Q000473:pathology; D009059:Mouth Diseases / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D053722:Receptors, Interleukin-17 / Q000378:metabolism*; D055815:Young Adult
C508717:IL17A protein, human; C109985:IL17RA protein, human; C486701:IL25 protein, human; D020381:Interleukin-17; D053722:Receptors, Interleukin-17