[Growth factors may enhance c-fos and c-jun gene expressions of hepatic stellate cells].
OBJECTIVE
To investigate the effects of growth factors on c-fos and c-jun gene expressions in hepatic stellate cells.


METHODS
Hepatic stellate cell (HSC) T6 was cultured in media containing different concentrations of platelet derived growth factor (PDGF) (8 ng/ml, 40 ng/ml or 200 ng/ml) and transforming growth factor (TGF) beta (0.2 ng/ml, 1 ng/ml or 5 ng/ml) and the cells were collected at different incubation periods (8, 24, 48 or 72 h). Total RNA of the HSC was isolated and c-fos and c-jun gene expression levels were measured by reverse transcription polymerase chain reaction.


RESULTS
C-fos gene expression levels of the HSC cultured in low (8 ng/ml), medium (40 ng/ml) and high (200 ng/ml) concentrations of PDGF were all much higher than those of the control group after exposure to PDGF at 8, 24, 48 or 72 h. The c-fos gene expression levels of the HSC increased as the dosage of PDGF increased and there were significant differences of c-fos gene expression among the three PDGF groups. C-jun gene expression levels of the HSC in low (0.2 ng/ml), medium (1.0 ng/ml) and high (5.0 ng/ml) concentrations of TGF beta groups were much higher than those of the control group after exposure to TGF beta at 8, 24, 48 or 72 h. The c-jun gene expression levels of the HSC increased as the dosage of TGF beta increased and there were significant differences of c-jun gene expression among the three TGF beta groups.


CONCLUSIONS
PDGF and TGF beta can strongly up-regulate c-fos and c-jun gene expressions in hepatic stellate T6 cells.
Wang
Ai-Min
AM
Department of Gastroenterology, PLA 252 Hospital, Baoding 071000, China. aiminwang62@sina.com
Ren
Chun-Hai
CH
Xiang
Ying
Y
D002478:Cells, Cultured; D015870:Gene Expression; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D010982:Platelet-Derived Growth Factor; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D016212:Transforming Growth Factor beta