Existence of splicing variants in homologues of Theileria lestoquardi clone-5 gene's transcripts in Theileria annulata and Theileria parva.
Clone 5 has been described as an immunogenic protein and was used to establish an ELISA for malignant theileriosis. Molecular characterization of the gene product revealed alternative splicing at the single intron resulting in two mRNA transcripts, translating into a long and a short protein form. Homologues of clone 5 exist in Theileria annulata and T. parva according to the available annotated GenBank sequences, showing however only the long protein forms in these parasites (GenBank accession numbers CAI73679, EAN33624). The present study aimed to determine whether two splice variants of homologues of clone 5 occur in T. annulata and T. parva.
Bakheit
Mohammed A
MA
Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan.
Ahmed
Jabbar S
JS
Seitzer
Ulrike
U
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D016794:Theileria / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D012333:RNA, Messenger