Scavenger receptor class B type I mediates cell entry of hepatitis C virus.
This study assessed the functional role of human scavenger receptor class B type I (SR-BI) as a putative hepatitis C virus (HCV) receptor using Chinese hamster ovary (CHO) cells transfected with human SR-BI (CHO-huSR-BI). The expression of SR-BI by primary Tupaia hepatocytes (PTHs), human hepatocarcinoma cell line (HepG2) cells, untransfected CHO cells and CHO-huSR-BI cells was analysed by Western blotting. Receptor competition assays showed that anti-SR-BI antibodies that block the binding of soluble envelope glycoprotein E2 could prevent HCV infection. Pre-incubation of CHO-huSR-BI and HepG2 cells with anti-SR-BI antibodies resulted in marked inhibition of E2 binding. After incubation with HCV RNA-positive serum from a patient with chronic HCV infection, however, HCV infection could not be detected in CHO-huSR-BI cells, but was detected in PTHs. These results demonstrate that, whilst SR-BI represents an important cell surface molecule for HCV infection, the presence of SR-BI alone is insufficient for HCV entry.
Jia
Z S
ZS
Centre of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. jiazsh@fmmu.edu.cn
Du
D W
DW
Lei
Y F
YF
Wei
X
X
Yin
W
W
Ma
L
L
Lian
J Q
JQ
Wang
P Z
PZ
Li
D
D
Zhou
Y X
YX
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D015153:Blotting, Western; D016466:CHO Cells / Q000378:metabolism / Q000821:virology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000821:virology*; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000821:virology*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000494:pharmacology; D051122:Scavenger Receptors, Class B / Q000502:physiology*; D014162:Transfection; D014417:Tupaia
D019138:Antibodies, Blocking; D012367:RNA, Viral; D051122:Scavenger Receptors, Class B