Study of spatial organization of chicken alpha-globin gene domain by 3C technique.
This work deals with 3C (Chromosome Conformation Capture) analysis of the chicken alpha-globin gene domain in embryonic erythrocytes and lymphoid cells. Ligation products were quantitatively analyzed by real-time PCR with TaqMan probes. It was found that in lymphoid cells, where alpha-globin gene is not active, the domain has a relatively extended configuration. In embryonic erythrocytes that transcribe alpha(D) and alpha(A) genes, simultaneous interaction of several domain elements was revealed including the major regulatory element, the erythroid-specific DNase I hypersensitive site at a distance of 9 kb upstream from the alpha-globin gene cluster (-9 DHS), promoter of the housekeeping gene CGTHBA, the alpha(D)-globin gene promoter, and the erythroid-specific enhancer located after the alpha-globin gene cluster. We suppose that such interaction is necessary to provide for the active transcription status of the chicken alpha-globin gene domains in erythroid cells.
Gavrilov
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AA
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia. aleksey.gavrilov@mail.ru
Razin
S V
SV
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods; D004742:Enhancer Elements, Genetic; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D055542:alpha-Globins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D055542:alpha-Globins