Bile affects the synthesis of exopolysaccharides by Bifidobacterium animalis.
By using cryo-scanning electron microscopy and quantification with lectin-conjugated probes, we have detected the production of exopolysaccharides (EPS) in Bifidobacterium animalis subsp. lactis in the presence of bile. In addition, the expression of gtf01207, which codifies a putative priming glycosyltransferase involved in EPS synthesis, was induced by bile.
Ruas-Madiedo
Patricia
P
Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Carretera de Infiesto s/n 33300 Villaviciosa, Asturias, Spain. ruas-madiedo@ipla.csic.es
Gueimonde
Miguel
M
Arigoni
Fabrizio
F
de los Reyes-Gavilán
Clara G
CG
Margolles
Abelardo
A
D001426:Bacterial Proteins / Q000096:biosynthesis; D001644:Bifidobacterium / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D001646:Bile / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D020869:Gene Expression Profiling; D005964:Glucosyltransferases / Q000096:biosynthesis; D037102:Lectins / Q000378:metabolism; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
D001426:Bacterial Proteins; D037102:Lectins; D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases