title: In vitro nematocidal activity of plant extracts of Mexican flora against Haemonchus contortus fourth larval stage.
abstract: Fourteen plant extracts were evaluated in vitro against the fourth larval stage of Haemonchus contortus. The plants species used were Tagetes erecta, Argemone mexicana, and Castela tortuosa. The assays were run in 24-well cell culture plates at room temperature with three replicates. After exposure, aliquots were taken from the corresponding wells and transferred to a microscope for observation. Evaluation criteria were based on the average of live and/or dead larvae. ANOVA test and Tukey test were used to determine significant differences among the treatments. After 96 h, the T. erecta acetonic extract produced 99.7% lethal activity, followed by C. tortuosa hexanic extract (85.8%) and T. erecta methanolic extract (58.3%) (P < 0.0001).
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitologia Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Morelos, México.
authors.forename: Hector Hugo Galicia
authors.initials: HH
authors.lastname: Aguilar
authors.forename: Pedro Mendoza
authors.initials: PM
authors.lastname: de Gives
authors.forename: David Osvaldo Salinas
authors.initials: DO
authors.lastname: Sánchez
authors.forename: María Eugenia López
authors.initials: ME
authors.lastname: Arellano
authors.forename: Enrique Liébano
authors.initials: EL
authors.lastname: Hernández
authors.forename: Ulises López
authors.initials: UL
authors.lastname: Aroche
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Valladares-Cisneros
mesh_terms: D000818:Animals; D001681:Biological Assay; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D009348:Nematoda / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D010936:Plant Extracts