Nitric oxide accumulation is required to protect against iron-mediated oxidative stress in frataxin-deficient Arabidopsis plants.
Frataxin is a mitochondrial protein that is conserved throughout evolution. In yeast and mammals, frataxin is essential for cellular iron (Fe) homeostasis and survival during oxidative stress. In plants, frataxin deficiency causes increased reactive oxygen species (ROS) production and high sensitivity to oxidative stress. In this work we show that a knock-down T-DNA frataxin-deficient mutant of Arabidopsis thaliana (atfh-1) contains increased total and organellar Fe levels. Frataxin deficiency leads also to nitric oxide (NO) accumulation in both, atfh-1 roots and frataxin null mutant yeast. Abnormally high NO production might be part of the defence mechanism against Fe-mediated oxidative stress.
Martin
Mariana
M
Instituto de Investigaciones Biológicas (IIB), Facultad de Ciencias Exactas y Naturales, CONICET-Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.
Colman
María José Rodríguez
MJ
Gómez-Casati
Diego F
DF
Lamattina
Lorenzo
L
Zabaleta
Eduardo Julián
EJ
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D010641:Phenotype; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D000096923:Frataxin
D007501:Iron; D033862:Iron-Binding Proteins; D009569:Nitric Oxide; D000096923:Frataxin