title: Expression of pro-angiogenic cytokines and their inhibition by dexamethasone in an ex vivo model of nasal polyps.
abstract: We used an organ culture of nasal polyps (NP) to provide ex vivo model to study the expression of pro-angiogenic cytokines and the effect of glucocorticoids in the pathogenesis of NP. Glucocorticoids are the drugs of choice for clinical treatment of NP; however, their mechanism of action is not fully understood. The cell-cell and cell-matrix integrity is well maintained in cultured NP. Expression of IL-6, IL-8, bFGF, GRO, and MCP-1 was increased in cultured NP compared to pre-cultured NP. Expression levels of IL-6, bFGF, and GRO in cultured NP were downregulated by dexamethasone (DEX) treatment, while MCP-1 expression was not suppressed. Further, RT-PCR and immunohistochemical analysis showed that HIF-1alpha and VEGF were suppressed in DEX-treated NP compared to untreated NP. Taken together, these results suggest that ex vivo organ culture can be considered a useful model to study the pathogenesis and regulation of pro-angiogenic cytokines in nasal polypogenesis.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Inje University, 633-165 Gae-Gum-dong, Busan-Jin-gu, Busan 614-735, Republic of Korea.
authors.forename: Seong Kook
authors.initials: SK
authors.lastname: Park
authors.forename: Won Hee
authors.initials: WH
authors.lastname: Jang
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Yang
mesh_terms: D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D003907:Dexamethasone / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008954:Models, Biological; D009298:Nasal Polyps / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009924:Organ Culture Techniques; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000096:biosynthesis
chemical_list: D016207:Cytokines; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; D003907:Dexamethasone