title: Chapter 14: Electron microscopy of hydrated samples.
abstract: Conventional electron microscopy offers a substantial resolution advantage over light microscopy, but requires difficult and often destructive preparation techniques. Recent advances in electron microscopy allow for imaging of hydrated samples, retaining the resolution advantage while removing the difficulty in preparation. Two new techniques, environmental scanning electron microscopy and wet electron microscopy offer this advantage, allowing for new possibilities in biological imaging.
authors.affiliation: Whitehead Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Winston
authors.initials: W
authors.lastname: Timp
authors.forename: Paul
authors.initials: P
authors.lastname: Matsudaira
mesh_terms: D000818:Animals; D002460:Cell Line; D019902:Chondrocytes / Q000648:ultrastructure; D004847:Epithelial Cells / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D014867:Water
chemical_list: D014867:Water