title: Three metabolites from the mangrove endophytic fungus Sporothrix sp. (#4335) from the South China Sea.
abstract: Three metabolites, sporothrins A, B, and C (1-3), were isolated from the mangrove endophytic fungus Sporothrix sp. (#4335). Their structures were identified by the spectral data and X-ray diffractive techniques, with compound 1 showing strong inhibition of acetylcholine esterase. 1,3,6,8-Tetrahydroxynaphthalene (T4HN) was deduced as one of the precursors in the biosynthesis of 1 and 2. In a primary biosynthesis gene study, the partial gene fragment obtained with the LC1-Im/2c-Im primer pair was shown to be closely related to genes encoding T4HN synthase. The deduced protein sequences were highly homologous to the ketosynthase domains of other fungal PKS genes involved in T4HN biosynthesis.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-Sen (Zhongshan) University, Guangzhou, 510275, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wen
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Cai
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: She
authors.forename: Wing Lai
authors.initials: WL
authors.lastname: Chan
authors.forename: L L P
authors.initials: LL
authors.lastname: Vrijmoed
authors.forename: E B G
authors.initials: EB
authors.lastname: Jones
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Lin
mesh_terms: D018360:Crystallography, X-Ray; D008958:Models, Molecular; D009284:Naphthols / Q000737:chemistry* / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D043982:Rhizophoraceae / Q000382:microbiology; D013175:Sporothrix / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009284:Naphthols