title: Alterations in detergent-resistant plasma membrane microdomains in Arabidopsis thaliana during cold acclimation.
abstract: Microdomains in the plasma membrane (PM) have been proposed to be involved in many important cellular events in plant cells. To understand the role of PM microdomains in plant cold acclimation, we isolated the microdomains as detergent-resistant plasma membrane fractions (DRMs) from Arabidopsis seedlings and compared lipid and protein compositions before and after cold acclimation. The DRM was enriched in sterols and glucocerebrosides, and the proportion of free sterols in the DRM increased after cold acclimation. The protein-to-lipid ratio in the DRM was greater than that in the total PM fraction. The protein amount recovered in DRMs decreased gradually during cold acclimation. Cold acclimation further resulted in quantitative changes in DRM protein profiles. Subsequent mass spectrometry and Western blot analyses revealed that P-type H(+)-ATPases, aquaporins and endocytosis-related proteins increased and, conversely, tubulins, actins and V-type H(+)-ATPase subunits decreased in DRMs during cold acclimation. Functional categorization of cold-responsive proteins in DRMs suggests that plant PM microdomains function as platforms of membrane transport, membrane trafficking and cytoskeleton interaction. These comprehensive changes in microdomains may be associated with cold acclimation of Arabidopsis.
authors.affiliation: Iwate University, Morioka, Japan.
authors.forename: Anzu
authors.initials: A
authors.lastname: Minami
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Akari
authors.initials: A
authors.lastname: Furuto
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Fukao
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kamo
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Matsuo
authors.initials: M
authors.lastname: Uemura
mesh_terms: D000064:Acclimatization; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000032:analysis; D003080:Cold Temperature; D003902:Detergents / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D021962:Membrane Microdomains / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods
chemical_list: D029681:Arabidopsis Proteins; D003902:Detergents; D008055:Lipids; D008565:Membrane Proteins