Murine hematopoietic stem cell transduction using retroviral vectors.
Hematopoietic stem cells (HSCs) represent an important target cell population in bone marrow transplantation and gene therapy applications. Their progeny cells carry the genetic information of the HSCs and replenish the blood and immune system. Therefore, in the setting of inherited diseases, transduction of HSCs with retroviral vectors (including gammaretro- and lentiviral vectors) offers the possibility to correct the phenotype in all blood lineages as demonstrated in clinical trials for immunodeficiencies (e.g., X-SCID). In the process of developing gene therapy strategies for patient applications, suitable mouse models for the human gene therapy are important to validate the concept. Stem-cell-enriched populations such as lineage negative cells as the functional equivalent of human CD34(+) cells can be isolated from murine bone marrow and efficiently transduced using retroviral vectors. This chapter provides a step-by-step protocol for retroviral transduction of murine lineage negative cells.
Modlich
Ute
U
Department of Experimental Hematology, Hannover Medical School, Hannover, Germany.
Schambach
Axel
A
Li
Zhixiong
Z
Schiedlmeier
Bernhard
B
D000818:Animals; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic