Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and respiratory syndrome virus (CA) and its protein expression by recombinant baculoviruses.
To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.
Koo
Hyun Na
HN
Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
Oh
Jeong Mi
JM
Lee
Jae Kyung
JK
Choi
Jae Young
JY
Lee
Kwang Sik
KS
Roh
Jong Yul
JY
Je
Yeon Ho
YH
Jin
Byung Rae
BR
Yoo
Sung Sik
SS
Kim
Jae Su
JS
Kim
Young In
YI
Yoon
In Joong
IJ
Woo
Soo Dong
SD
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D007723:Korea; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011995:Recombination, Genetic; D016415:Sequence Alignment; D018411:Spodoptera / Q000821:virology; D013552:Swine; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*
D015678:Viral Structural Proteins