LCMS and GCMS for the screening of alkaloids in natural and in vitro extracts of Leucojum aestivum.
HPLC coupled to a mass spectrometer (MS) was used for the analysis of galanthamine and lycorine in natural extracts of Leucojum aestivum and in their in vitro cultures grown with a precursor (ACC), inhibitors (AgNO(3), STS), or an absorber (KMnO(4)) of ethylene. The maximum galanthamine (0.002%) and lycorine (0.02%) concentrations in tissue cultures were obtained in the presence of KMnO(4). GCMS was used to investigate underivatized alkaloid mixtures from L. aestivum. Seven alkaloids were identified in in vivo bulbs. KMnO(4) led to the highest diversity of alkaloids in tissue culture extracts.
Ptak
Agata
A
Department of Plant Breeding and Seed Science, Agricultural University, 31-140 Krakow, Poland.
El Tahchy
Anna
A
Dupire
François
F
Boisbrun
Michel
M
Henry
Max
M
Chapleur
Yves
Y
Moś
Maria
M
Laurain-Mattar
Dominique
D
D047151:Amaryllidaceae Alkaloids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry
D047151:Amaryllidaceae Alkaloids