title: Clinical significance of anti-HEV IgA in diagnosis of acute genotype 4 hepatitis E virus infection negative for anti-HEV IgM.
abstract: Anti-HEV IgM is a diagnostic for recent or ongoing HEV infection. However, some patients with acute hepatitis E (AHE) negative for anti-HEV IgM in acute period were often observed in clinical practice. In this study, we constructed the anti-HEV IgA indirect ELISA assay to evaluate the significance of anti-HEV IgA. The specificity of anti-HEV IgA was 99.6%. Among 245 AHE patients, 84 samples from 84 patients were positive for HEV RNA. The positive rate of anti-HEV IgA, anti-HEV IgM and anti-HEV IgG in 84 samples positive for HEV RNA was 96.3, 97.6, and 88.1%, respectively, and no sample was negative for anti-HEV IgA and anti-HEV IgM simultaneously. Among 245 AHE patients, we found nine samples collected from nine patients in acute period were negative for anti-HEV IgM but positive for anti-HEV IgA and two samples were positive for HEV RNA. Detection of anti-HEV IgA can be a useful supplement for diagnosis of acute HEV infection especially in patients negative for anti-HEV IgM.
authors.affiliation: Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Zhang
authors.forename: Deying
authors.initials: D
authors.lastname: Tian
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Junhui
authors.initials: J
authors.lastname: Xiong
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Ge
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ningshao
authors.initials: N
authors.lastname: Xia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood* / Q000276:immunology; D018963:Hepatitis Antigens / Q000276:immunology*; D016751:Hepatitis E / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D016752:Hepatitis E virus / Q000235:genetics; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D006508:Hepatitis Antibodies; D018963:Hepatitis Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral