title: Cu,Zn-superoxide dismutase is necessary for proper function of VDAC in Saccharomyces cerevisiae cells.
abstract: Available data suggest that a copper-and zinc-containing dismutase (CuZnSOD) plays a significant role in protecting eukaryotic cells against oxidative modifications which may contribute to cell aging. Here we demonstrated that depletion of CuZnSOD in Saccharomyces cerevisiae cells (Deltasod1 cells) affected distinctly channel activity of VDAC (voltage dependent anion selective channel) and resulted in a moderate reduction in VDAC levels as well as in levels of protein crucial for VDAC import into mitochondria, namely Tob55/Sam50 and Tom40. The observed alterations may result in mitochondriopathy and subsequently in the shortening of the replicative life span observed for S. cerevisiaeDeltasod1 cells.
authors.affiliation: Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Andonis
authors.initials: A
authors.lastname: Karachitos
authors.forename: Hanna
authors.initials: H
authors.lastname: Galganska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Wojtkowska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Budzinska
authors.forename: Olgierd
authors.initials: O
authors.lastname: Stobienia
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.forename: Hanna
authors.initials: H
authors.lastname: Kmita
mesh_terms: D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; C487333:Sam50 protein, S cerevisiae; C066555:Tom40 protein, S cerevisiae; D050994:Voltage-Dependent Anion Channels; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1