title: UCP3 overexpression neutralizes oxidative stress rather than nitrosative stress in mouse myotubes.
abstract: The deleterious effects of oxidants on proteins may be modified by overexpression of uncoupling protein 3 (UCP3) in skeletal muscle cells exposed to hyperoxia or H(2)O(2). UCP3 overexpression significantly attenuated the increase in protein carbonylation in response to hyperoxia and H(2)O(2) exposures. However, antioxidant enzyme content and activity (superoxide dismutases, peroxiredoxins, glutathione peroxidase-I, and catalase) were reduced or not modified in UCP3-overexpressing myotubes exposed to oxidants. Protein nitration increased in UCP3-overexpressing cells exposed to hyperoxia, but not to H(2)O(2). We conclude that protein oxidation rather than nitration is neutralized by UPC3 overexpression in mouse myotubes exposed to abundant reactive oxygen species.
authors.affiliation: Muscle and Respiratory System Research Unit-Pneumology Department, IMIM-Hospital del Mar, Centro de Investigación en Red de Enfermedades Respiratorias (CibeRes), CEXS, Universitat Pompeu Fabra, Barcelona Biomedical Research Park, Barcelona, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Barreiro
authors.forename: Cèlia
authors.initials: C
authors.lastname: Garcia-Martínez
authors.forename: Sandra
authors.initials: S
authors.lastname: Mas
authors.forename: Elisabet
authors.initials: E
authors.lastname: Ametller
authors.forename: Joaquim
authors.initials: J
authors.lastname: Gea
authors.forename: Josep M
authors.initials: JM
authors.lastname: Argilés
authors.forename: Sílvia
authors.initials: S
authors.lastname: Busquets
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: López-Soriano
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007473:Ion Channels / Q000096:biosynthesis*; D051379:Mice; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D015538:Nitrosation; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D009566:Nitrates; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3; D014443:Tyrosine; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase