Cost-effective method for growing three-dimensional cell cultures in extracellular matrix extract.
Three-dimensional (3-D) cell cultures are advantageous for modeling complex cellular behavior. A major issue with 3-D cultures grown in Engelbreth-Holm-Swarm (EHS) extracellular matrix extract, however, is the expense. We describe here a simple method using a rubber gasket laid over a coverslip, which provides sufficient 3-D area filled with a minimal amount of EHS, resulting in cost-effective 3-D cellular cultures that are easy to manipulate.
Jacob
A I
AI
Genomic Medicine Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
Waite
K A
KA
Eng
C
C
D000818:Animals; D018929:Cell Culture Techniques / Q000191:economics / Q000379:methods*; D002460:Cell Line; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry; D005109:Extracellular Matrix
D003470:Culture Media