Via zinc(II) protoporphyrin to the synthesis of poly(ZnPP-MAA-EGDMA) for the imprinting and selective binding of bilirubin.
Poly(zinc protoporphyrin-methacrylic acid-ethyl glycol dimethylacrylate) (poly(ZnPP-MAA-EGDMA)) imprinted with alpha-bilirubin can cause spectroscopic change in wavelength and absorption intensity due to the metal-ion coordination between ZnPP and bilirubin. The fluorescent imprinted polymer was able to selectively bind alpha-bilirubin. The corresponding imprinted polymer monolith was synthesized by using the functional monomer, methacrylic acid and the fluorescent monomer, zinc(II) protoporphyrin. Although the imprinted polymers (MIPs) using methacrylic acid, protoporphyrin, or zinc(II) protoporphyrin alone as the only functional monomer could bind bilirubin, the imprinting effects were all comparably inferior to the imprinted poly(ZnPP-MAA-EGDMA). Therefore, it revealed that via the combined utilization of ZnPP and MAA for the fluorescent and functional effect, the MIPs thus prepared were then able to create the highly selective cavities. The optimal condition for the heated polymerization of the imprinted poly(ZnPP-MAA-EGDMA) was found to be 60 degrees C for 6 h. The imprinting factor of 3.069 could be achieved from the fluorescent imprinted polymer by comparing the binding results obtained from the MIP and the NIP (non-imprinted polymer). The imprinting factor obtained from bilirubin/biliverdin mixture solution was reduced to 2.111 because of the presence of biliverdin. The selectivity toward bilirubin of 2.269 from the bilirubin/biliverdin mixture was obtained. Therefore, to utilize ZnPP for the preparation of the imprinted materials confirmed the selective binding and detection of bilirubin via the fluorescent approach.
Chou
Shih-Kai
SK
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Syu
Mei-Jywan
MJ
D001663:Bilirubin / Q000737:chemistry*; D001664:Biliverdine / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D011524:Protoporphyrins / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011108:Polymers; D011524:Protoporphyrins; C017803:zinc protoporphyrin; C004919:ethylene dimethacrylate; D001664:Biliverdine; D001663:Bilirubin