Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death.
Glutathione-S-transferases are essential proteins involved in cellular detoxification. The expression of GSTs has been studied extensively under various environmental stressors including xenobiotics. Here, we have isolated AtGST10, one of the phi classes of AtGSTs on the basis of its interaction with BAK1 in a yeast two-hybrid screen. BAK1 is an LRR-RLK, acting in both brassinosteroid signaling and plant defense responses. We found that AtGSTF10 binds to BAK1 through its N-terminal domain. AtGSTF10 is expressed ubiquitously in plant tissues, and the endogenous transcript level of AtGSTF10 was not induced by plant growth regulators or abiotic stressors, except drought, unlike other GSTs. Overexpression of AtGSTF10 conferred higher tolerance to salt and disturbed redox status of transgenic plants. The down-regulation of AtGSTF10 produced by RNA interference caused reduced tolerance to abiotic stress and an accelerated senescence of transformants, indicating that AtGSTF10 is involved in stress tolerance and the BAK1-mediated spontaneous cell death signaling pathway in Arabidopsis.
Ryu
Hee Young
HY
Division of Biological Science, Sookmyung Women's University, 53-12 Chungpa-dong 2ga, Yongsan-gu, Seoul 140-742, Republic of Korea.
Kim
Sun Young
SY
Park
Hyun Mi
HM
You
Ji Young
JY
Kim
Beg Hab
BH
Lee
June Seung
JS
Nam
Kyoung Hee
KH
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D016922:Cellular Senescence; D015536:Down-Regulation; D055864:Droughts; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; D005982:Glutathione Transferase; C463660:BAK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases