title: Comparison of two versions of larval development test to detect anthelmintic resistance in Haemonchus contortus.
abstract: Larval development (LDT) and micro-agar larval development tests (MALDT) were used to compare the reliability and sensitivity of two methods for detecting anthelmintic resistance in Haemonchus contortus. The tests were conducted using three resistant and four susceptible isolates of H. contortus. Both versions of the tests provided comparable results with regard to the characterization of benzimidazole and levamisole susceptibility but neither test was sufficiently sensitive to discrimination between an ivermectin (IVM) susceptible and an IVM resistant isolate. Each test has its own merits with the LDT having the advantage of being less time-consuming.
authors.affiliation: Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Kosice, Slovak Republic. varady@saske.sk
authors.forename: Marián
authors.initials: M
authors.lastname: Várady
authors.forename: Július
authors.initials: J
authors.lastname: Corba
authors.forename: Valéria
authors.initials: V
authors.lastname: Letková
authors.forename: Gabriel
authors.initials: G
authors.lastname: Kovác
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology* / Q000627:therapeutic use; D001562:Benzimidazoles / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D006188:Haemonchiasis / Q000188:drug therapy / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects*; D066298:In Vitro Techniques; D007559:Ivermectin / Q000494:pharmacology / Q000627:therapeutic use; D007814:Larva; D007928:Lethal Dose 50; D010270:Parasite Egg Count / Q000662:veterinary; D021261:Parasitic Sensitivity Tests / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D007559:Ivermectin; C031000:benzimidazole