title: Prevalence of Epstein-Barr virus type 1 in patients with chronic periodontitis by nested-PCR.
abstract: The present study evaluated the subgingival presence of Epstein-Barr virus type 1 (EBV-1) in patients with chronic periodontitis with nested-PCR. Subgingival plaque samples from 61 patients with chronic periodontitis with Probing Depth (PD) > or = 6 and 40 healthy controls were collected by sterile curette. DNA was extracted. A nested Polymerase Chain Reaction (PCR) method determined presence of EBV-1. The study included 61 patients (34 women, 27 men; 24-69 years of age; mean 43) and 40 periodontally health controls (22 Women, 18 men, 21-69 years in age; mean 41.35%). EBV type 1 was detected in 37 samples (60.7%) and 1 samples (2.5%) of chronic periodontitis patients and healthy subjects, respectively. This study demonstrated that EBV-1 infection is associated with the activity of chronic periodontitis.
authors.affiliation: Department of Microbiology, Isfahan Medical University, Isfahan, Iran.
authors.forename: S H
authors.initials: SH
authors.lastname: Moghim
authors.forename: M
authors.initials: M
authors.lastname: Chalabi
authors.forename: A Moghareh
authors.initials: AM
authors.lastname: Abed
authors.forename: F
authors.initials: F
authors.lastname: Rezaei
authors.forename: H
authors.initials: H
authors.lastname: Tamizifar
mesh_terms: D000328:Adult; D000368:Aged; D002908:Chronic Disease; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D003773:Dental Plaque / Q000821:virology*; D005260:Female; D005784:Gene Amplification; D005814:Genes, Viral; D005881:Gingiva / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
chemical_list: D017931:DNA Primers; D004279:DNA, Viral