title: [Influence of various glucose levels on the structure and function of cultured neonatal rats cardiomyocytes].
abstract: OBJECTIVE
  To study the influence of various glucose levels on the structure and function of cultured neonatal rats cardiomyocytes.
  
  
  METHODS
  Cultured neonatal ventricular cardiomyocytes were treated with various glucose levels for 5 days: control (5.5 mmol/L); high (25.5 mmol/L); intermittent high (5.5 mmol/L or 25.5 mmol/L in every 12 hours interval); high (25.5 mmol/L) + PKC inhibitor Ro-31-8220 (50 nmol/L). Then, the cell beating frequency was counted, the cardiomyocytes diameters were measured and the expressions of PKC-alpha, PKC-beta(2), p-PKC-alpha, p-PKC-beta(2), NF-kappaB and c-fos were determined by Western blot.
  
  
  RESULTS
  Compared with control group, cardiomyocytes beating frequency, diameters as well as the expressions of PKC-alpha, PKC-beta(2), p-PKC-alpha, p-PKC-beta(2), NF-kappaB and c-fos were significantly increased in high glucose concentration (all P < 0.05) and intermittent high glucose treatment further amplified these changes (all P < 0.05 vs. high glucose and control groups). High glucose induced changes could be significantly attenuated with PKC inhibitor Ro-31-8220.
  
  
  CONCLUSIONS
  High, especially intermittent high glucose could lead to diabetic cardiomyopathy by promoting cardiac hypertrophy, increasing beating frequency via activating PKC/NF-kappaB/c-fos pathways.
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Zhang
authors.forename: Bin-quan
authors.initials: BQ
authors.lastname: Zhou
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Fu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016328:NF-kappa B; D011493:Protein Kinase C; D005947:Glucose