Complexing of glucose oxidase with anti-glucose oxidase antibodies or the F(ab)'(2)/F(ab)' fragments derived therefrom protects both the enzyme and antibody/antibody fragments against glycation.
Incubation of Aspergillus niger glucose oxidase with glucose, fructose, or ribose results in remarkable inactivation of the enzyme. Glucose oxidase incubated with the sugars migrated as a diffuse band of low intensity and silver stained poorly after SDS-PAGE. Purified anti-glucose oxidase antibodies and F(ab)'(2) or F(ab)' derived therefrom were effective in restricting the inactivation of the enzyme induced by the sugars, providing up to 90% protection. The sugars also caused remarkable changes in the electrophoretic behavior of anti-glucose oxidase antibodies and the fragments, but complexing with glucose oxidase restricted the changes both in the enzyme and the antibody/antibody fragments.
Jairajpuri
D S
DS
Department of Biochemistry, Aligarh Muslim University, Aligarh, 202002, India.
Fatima
S
S
Saleemuddin
M
M
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D001234:Aspergillus niger / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D005949:Glucose Oxidase / Q000276:immunology / Q000378:metabolism*; D006031:Glycosylation; D007128:Immunoglobulin Fragments / Q000276:immunology / Q000378:metabolism*; D009005:Monosaccharides / Q000378:metabolism; D011817:Rabbits
D000906:Antibodies; D001426:Bacterial Proteins; D007128:Immunoglobulin Fragments; D009005:Monosaccharides; D005949:Glucose Oxidase