Genotype and antibiotic susceptibility patterns of drug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates in Taiwan.
Rapid and accurate identification of the drug susceptibility profile of clinical strains is very important for controlling bacterial infections and determining the antibiotic therapy. The objective of this study was to investigate the spectrum of the correlation between phenotypic and genetic characters of the drug-resistant clinical isolates. A total of 133 clinical isolates, including 76 Acinetobacter baumannii and 57 Pseudomonas aeruginosa, were examined for their antibiotic susceptibility by the method of disc diffusion. Among them, most of the isolates were multiresistant, and 80% of the strains showed phenotypic resistance to beta-lactam antibiotics. Using PCR analysis, among the several types of beta-lactamases, TEM was the most prevalent, and OXA was the second most prevalent. The integron harbored was identified by conserved segment PCR, and 50% of the test isolates carried integrons with various gene cassette sizes inserted. The results obtained from this study reveal that the majority of these isolates displayed multiple drug resistance phenotypes that were associated with their mutational gene profiles.
Yang
Cheng-Hong
CH
Department of Electronic Engineering, National Kaohsiung University of Applied Sciences, Kaohsiung, Taiwan, Republic of China.
Lee
Shaoyung
S
Su
Pai-Wei
PW
Yang
Cheng-San
CS
Chuang
Li-Yeh
LY
D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015995:Prevalence; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D013624:Taiwan / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases