Pseudomonas putida based amperometric biosensors for 2,4-D detection.
Amperometric biosensors using Pseudomonas putida cells as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). After the adaptation process of Pseudomonas putida to 2,4-D, cells were immobilized onto the screen printed graphite electrodes (SPG) as well as Clark oxygen probe by gelatin and glutaraldehyde. Optimum pH, temperature, and stability of the biosensor were investigated. Substrate specificities for various phenolic compounds were also searched. In repeatability studies, variation coefficients and standard deviations for both type of systems were calculated; SPG and Clark electrodes were calculated and results are given as a comparison of two systems. Finally, the biosensors were applied to 2,4-D determination in a real herbicide sample.
Odaci
Dilek
D
Faculty of Science, Biochemistry Department, Ege University, Bornova-Izmir, Türkiye.
Sezgintürk
Mustafa Kemal
MK
Timur
Suna
S
Pazarlioğlu
Nurdan
N
Pilloton
Roberto
R
Dinçkaya
Erhan
E
Telefoncu
Azmi
A
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000494:pharmacology*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016958:Pseudomonas putida / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015084:2,4-Dichlorophenoxyacetic Acid