Differential activation of mitogen-activated protein kinases following high and low LET radiation in murine macrophage cell line.
Mitogen-activated protein kinases have been shown to respond to various stimuli including cytokines, mitogens and gamma irradiation, leading to cell proliferation, differentiation, or death. The duration of their activation determines the specificity of response to each stimulus in various cells. In this study, the crucial intracellular kinases, ERK, JNK, and p38 kinase involved in cell survival, death, or damage and repair were examined for their activity in RAW 264.7 cells at various time points after irradiation with 2 Gy doses of proton ions or X-rays. This is the first report that shows that the MAPK signaling induced after heavy ion or X-ray exposure is not the same. Unlike gamma irradiation, there was prolonged but marginal activation of prosurvival ERK pathway and significant activation of proapoptotic p38 pathway in response to high LET radiation.
Narang
Himanshi
H
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India.
Bhat
Nagesh
N
Gupta
S K
SK
Santra
S
S
Choudhary
R K
RK
Kailash
S
S
Krishna
Malini
M
D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D004307:Dose-Response Relationship, Radiation; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D008264:Macrophages / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism / Q000528:radiation effects*; D011522:Protons; D015398:Signal Transduction / Q000528:radiation effects; D013997:Time Factors; D014965:X-Rays; D048051:p38 Mitogen-Activated Protein Kinases
D011522:Protons; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases