Proton gradients produced by glucose oxidase microcapsules containing motor F0F1-ATPase for continuous ATP biosynthesis.
Glucose oxidase (GOD) microcapsules held together by cross-linker, glutaraldehyde (GA), are fabricated by the layer-by-layer (LbL) assembly technique. The lipid bilayer containing CF(0)F(1)-ATPase was coated on the outer shell of GOD microcapsules. Driven under the proton gradients produced by catalysis of GOD microcapsules for glucose, ATP is synthesized from ADP and inorganic phosphate catalyzed by the ATPase rotary catalysis. The results show here that ATPase reconstituted on the GOD microcapsules retains its catalytic activity.
Duan
Li
L
Beijing National Laboratory for Molecular Science, International Joint Laboratory, CAS Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Qi
Wei
W
Yan
Xuehai
X
He
Qiang
Q
Cui
Yue
Y
Wang
Kewei
K
Li
Dongxiang
D
Li
Junbai
J
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001234:Aspergillus niger / Q000201:enzymology; D002214:Capsules / Q000737:chemistry; D004795:Enzyme Stability; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011522:Protons
D002214:Capsules; D011522:Protons; D000255:Adenosine Triphosphate; D005949:Glucose Oxidase; D006180:Proton-Translocating ATPases