title: Cellular gene expression profiles in rhesus macaques challenged mucosally with a pathogenic R5 tropic simian human immunodeficiency virus isolate.
abstract: Insights into the host factors that contribute to an effective antiviral immune response may be obtained by examining global gene expression in simian human immunodeficiency virus (SHIV)-infected nonhuman primates that exhibit different virological outcomes. Immune responses and gene expression profiles in peripheral blood mononuclear cells (PBMCs) were compared between animals that controlled or did not control viremia after infection. Rectal inoculation of eight rhesus macaques with R5-tropic SHIV(SF162P3) resulted in a high level of plasma viremia during the acute phase of infection. The viremia was controlled to below levels of detection in six of these animals at the set point (controllers), whereas two animals had persistent viremia throughout the 140 wk that the animals were monitored (non-controllers). CD4(+) T-cell counts declined slightly in both controllers and non-controllers in the acute phase of infection, but CD4(+) T-cell counts continued to decline only in the non-controllers. Neutralizing antibodies to the challenge virus were variable and could not account for the control of viremia. However, analysis of the cellular gene expression profiles in the PBMCs from both groups of animals revealed distinctive gene expression patterns between controllers and non-controllers. Using the paired LPE test, 59 genes with p values <0.01 were identified and specific differences in the gene expression profiles in PBMCs from controllers versus non-controllers were detected.
authors.affiliation: Advanced BioScience Laboratories, Inc., Kensington, Maryland 20895, USA. hye.chung@ablinc.com
authors.forename: Hye-kyung
authors.initials: HK
authors.lastname: Chung
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Pise-Masison
authors.forename: Michael F
authors.initials: MF
authors.lastname: Radonovich
authors.forename: John
authors.initials: J
authors.lastname: Brady
authors.forename: Jae K
authors.initials: JK
authors.lastname: Lee
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Cheon
authors.forename: Phillip
authors.initials: P
authors.lastname: Markham
authors.forename: Anthony
authors.initials: A
authors.lastname: Cristillo
authors.forename: Ranajit
authors.initials: R
authors.lastname: Pal
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018791:CD4 Lymphocyte Count; D020869:Gene Expression Profiling; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology; D008253:Macaca mulatta; D020411:Oligonucleotide Array Sequence Analysis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; D015483:HIV Antibodies