[Epidemiological surveillance of measles occurrence in Poland in 2006 by means of virological and molecular methods].
The availability of sensitive cell line for isolation of measles virus from clinical samples and establishment RT-PCR and molecular sequencing metods have allowed for rapid genetic characterization of wild-type strains of measles virus. This sequence information makes it possible to identify the source of wild-type viruses and differentiate between native and reported cases. The aim of this study was evaluation of virus isolation in cell culture in National Laboratory in Poland (Department of Virology in NIH), presentation of RT-PCR and molecular genotyping results performed in Regional Reference Laboratory in Berlin (Robert Koch Institut).
Makówka
Agata
A
Zakład Wirusologii, Narodowy Instytut Zdrowia Publicznego-Państwowy Zakład Higieny, Warszawa. amakowka@pzh.gov.pl
Litwińska
Bogumiła
B
D016021:Epidemiologic Studies; D016679:Genome, Viral; D006801:Humans; D008457:Measles / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D008459:Measles virus / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011044:Poland / Q000453:epidemiology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048348:Reverse Transcription; D017422:Sequence Analysis, DNA
D012367:RNA, Viral