A preliminary study on genotypic differences in transcript abundance of drought-responsive genes in sugar beet.
In this study, four sugar beet genotypes of differing responses to drought were selected from a field experiment conducted under well-watered and water-limited conditions in 2004. In addition, two candidate genes: 2-cysteine peroxiredoxin (2-cys prx) and Nucleoside Diphosphate Kinase (NDPK), thought to be associated with drought tolerance, were chosen from a previous proteomics study in sugar beet. An expression analysis of the two drought-regulated genes using semi-quantitative reverse transcription Polymerase Chain Reaction (RT-PCR) indicated that there were genotypic differences in the transcript abundance of the candidate genes with the differences in the expression level of 2-cys prx being likely associated with the drought responses of the genotypes in a two-year field study. However, the expression analysis of the genes has to be investigated at different stages of the stress period on more genotypes.
Rajabi
Abazar
A
Department of Plant Breeding, Sugar Beet Seed Institute, P.O. Box 31585-4114, Karaj, Iran.
Ranji
Zabihallah
Z
Griffiths
Zhoward
Z
Ober
Eric S
ES
D027461:Beta vulgaris / Q000201:enzymology / Q000235:genetics*; D055864:Droughts; D005838:Genotype; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000378:metabolism; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D014158:Transcription, Genetic
D010940:Plant Proteins; D054464:Peroxiredoxins; D009701:Nucleoside-Diphosphate Kinase