title: Another formula for calculating the gene change rate in real-time RT-PCR.
abstract: Relative quantification real-time PCR has become a routine method in molecular biology research to study the small amount gene expression. There are a few mathematical models for relative quantification data analysis in real-time PCR, and the formula 2(-DDCT ) is one of the most frequently used method. In this paper, we are to present another equation which directly calculates the change rate (R (c)) of gene expression, and give a example to compare the application of this model with 2(-DDCT).
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, Soochow University, 215006 Suzhou, China.
authors.forename: Shujian
authors.initials: S
authors.lastname: Chang
authors.forename: Weichang
authors.initials: W
authors.lastname: Chen
authors.forename: Jicheng
authors.initials: J
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D015870:Gene Expression; D019073:HT29 Cells; D006801:Humans; D008957:Models, Genetic; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012333:RNA, Messenger