Multilayer tendon slices seeded with bone marrow stromal cells: a novel composite for tendon engineering.
The ideal scaffold for tendon engineering would possess the basic structure of the tendon, native extracellular matrix, and capability of cell seeding. The purpose of this study was to assess the tissue engineering potential of a novel composite consisting of a decellularized multilayer sliced tendon (MST) scaffold seeded with bone marrow stromal cells (BMSC). BMSC and infraspinatus tendons were harvested from 20 dogs. The tendons were sectioned in longitudinal slices with a thickness of 50 microm. The slices were decellularized, seeded with BMSC, and then bundled into one composite. The composite was incubated in culture media for 14 days. The resulting BMSC-seeded MST was evaluated by qRT-PCR and histology. The BMSC viability was assessed by a fluorescent tracking marker. Histology showed that the seeded cells aligned between the collagen fibers of the tendon slices. Analysis by qRT-PCR showed higher tenomodulin and MMP13 expression and lower collagen type I expression in the composite than in the BMSC before seeding. BMSC labeled with fluorescent tracking marker were observed in the composite after culture. Mechanical testing showed no differences between scaffolds with or without BMSC. BMSC can survive in a MST scaffold. The increased tenomodulin expression suggests that BMSC might express a tendon phenotype in this environment. This new composite might be useful as a model of tendon tissue engineering.
Omae
Hiromichi
H
Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 First Street S.W., Rochester, Minnesota 55905, USA.
Zhao
Chunfeng
C
Sun
Yu Long
YL
An
Kai-Nan
KN
Amadio
Peter C
PC
D000818:Animals; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells / Q000166:cytology*; D002470:Cell Survival / Q000502:physiology; D024042:Collagen Type I / Q000235:genetics; D024061:Collagen Type III / Q000235:genetics; D004285:Dogs; D015870:Gene Expression / Q000502:physiology; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D009924:Organ Culture Techniques; D012038:Regeneration / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology*; D013710:Tendons / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D016474:Weight-Bearing / Q000502:physiology
D024042:Collagen Type I; D024061:Collagen Type III; D053509:Matrix Metalloproteinase 13; D020778:Matrix Metalloproteinase 2