Transport behavior of ellagic acid of pomegranate leaf tannins and its correlation with total cholesterol alteration in HepG2 cells.
The aim of this study was to investigate whether ellagic acid in pomegranate leaf tannins could be transported into HepG2 cells and its transport behavior. High-performance liquid chromatography coupled with a 996 photodiode array detector at 254 nm was applied. The mobile phase was an acetonitrile-water solution (containing 0.1% triethylamine, pH 3.0; 16:64, v/v, for determining ellagic acid in cells). The flow rate was 0.8 mL/min. Cells were incubated with pomegranate leaf tannins with 100 and 50 microg/mL (containing 1.71 and 0.85 microg/mL of ellagic acid, respectively) for a specific time, then lysed and sonicated in methanol to extract intracellular ellagic acid. A 10 microL aliquot of sample was injected into the HPLC system to determine ellagic acid concentration. The results showed that ellagic acid in pomegranate leaf tannins could be transported into the cells, which was in correlation with total cholesterol alteration in the cells. This is the first time that the transport behavior of ellagic acid through HepG2 cells in vitro has been comprehensively demonstrated.
Lan
Jiaqi
J
Department of Biological Sciences and Biotechnology, Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing, People's Republic of China.
Lei
Fan
F
Hua
Lei
L
Wang
Yugang
Y
Xing
Dongming
D
Du
Lijun
L
D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D002784:Cholesterol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D004610:Ellagic Acid / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D029561:Lythraceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013634:Tannins / Q000737:chemistry; D013997:Time Factors
D013634:Tannins; D004610:Ellagic Acid; D002784:Cholesterol