A novel Taenia solium protein that resembles troponin T proteins.
Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5' end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis.
Mayta
Holger
H
Departamento de Microbiologia, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porres, Lima 31, Peru.
Hancock
Kathy
K
Gilman
Robert H
RH
Zamudio
Roxana
R
Castillo
Janeth P
JP
Levine
Min Z
MZ
Garcia
Hector H
HH
Gonzalez
Armando E
AE
Tsang
Victor C W
VC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003551:Cysticercosis / Q000175:diagnosis; D018076:DNA, Complementary / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein; D041201:Taenia solium / Q000737:chemistry* / Q000235:genetics; D013622:Taeniasis / Q000175:diagnosis*; D020107:Troponin T / Q000737:chemistry* / Q000235:genetics
D018076:DNA, Complementary; D015801:Helminth Proteins; D011994:Recombinant Proteins; D020107:Troponin T