title: No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions.
abstract: GPRC6A is a seven-transmembrane receptor mediating signaling by a wide range of L-alpha-amino acids, a signaling augmented by the divalent cations Ca(2)(+) and Mg(2)(+). GPRC6A transcripts are detected in numerous mammalian tissues, but the physiological role of the receptor is thus far elusive. Analogously to the closely related calcium-sensing receptor, GPRC6A has been proposed to function as a metabolic sensor of Ca(2)(+) and amino acids in bone and other tissues. In the present study, we have generated the first GPRC6A knockout mice and studied their phenotype with particular focus on bone homeostasis. The generated GPRC6A knockout mice are viable and fertile, develop normally, and exhibit no significant differences in body weight compared with wild-type littermates. Assessment of bone mineral density, histomorphometry, and bone metabolism demonstrated no significant differences between 13-week-old knockout and wild-type mice. In conclusion, our data do not support a role for GPRC6A in normal bone physiology.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark. pw@farma.ku.dk
authors.forename: Petrine
authors.initials: P
authors.lastname: Wellendorph
authors.forename: Lars Dan
authors.initials: LD
authors.lastname: Johansen
authors.forename: Anders A
authors.initials: AA
authors.lastname: Jensen
authors.forename: Emilio
authors.initials: E
authors.lastname: Casanova
authors.forename: Martin
authors.initials: M
authors.lastname: Gassmann
authors.forename: Pierre
authors.initials: P
authors.lastname: Deprez
authors.forename: Philippe
authors.initials: P
authors.lastname: Clément-Lacroix
authors.forename: Bernhard
authors.initials: B
authors.lastname: Bettler
authors.forename: Hans
authors.initials: H
authors.lastname: Bräuner-Osborne
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D005838:Genotype; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D010641:Phenotype; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013977:Tibia / Q000033:anatomy & histology / Q000378:metabolism
chemical_list: C515750:GPRC6A protein, mouse; D043562:Receptors, G-Protein-Coupled