[Increased expression of transient receptor potential melastatin 7 in mouse cardiac fibroblasts post myocardial infarction].
OBJECTIVE
To observe transient receptor potential melastatin 7-like (TRPM7L) expression changes post myocardial infarction (MI) in mouse cardiac fibroblast (CF).


METHODS
TRPM7 expression and Ca2+ influx in CF from MI and control mice were quantified by mRNA RT-PCR and whole cell patch clamp technique.


RESULTS
(1) TRPM7 expression was significantly upregulated post MI and Ca2+ influx of CF were significantly increased post MI [(7.4 +/- 0.7) pA/pF vs. (16.2 +/- 1.7) pA/pF, P < 0.01] and Ca2+ influx of CF increased 3-fold under lower pH condition; (2) These effects could be blocked by knock-out TRPM7 gene with SiRNA.


CONCLUSIONS
TRPM7L upregulation post MI and under lower pH condition are responsible for increased Ca2+ influx in CF.
Li
Ming-jiang
MJ
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China. li_mingjiang@hotmail.com
Zhou
Yong-ming
YM
Tang
Yan-hong
YH
Cao
Feng
F
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D051379:Mice; D032386:Myoblasts, Cardiac / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000235:genetics; D050053:TRPM Cation Channels / Q000378:metabolism*
D012333:RNA, Messenger; D050053:TRPM Cation Channels; C421903:Trpm7 protein, mouse; D002118:Calcium