[Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910].
OBJECTIVE
To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.


METHODS
The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.


RESULTS
Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).


CONCLUSIONS
The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.
Liu
Qun
Q
PLA Institute of Clinical Laboratory Medicine, Nanjing University School of Medicine/Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
Li
Fang-Qiu
FQ
Han
Yan-Ling
YL
Wang
Li-Kui
LK
Hou
Ya-Yi
YY
D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C090172:SPA17 protein, human