title: Diagnosis and prevalence of enteropathogenic bacteria in children less than 5 years of age with acute diarrhea in Tehran children's hospitals.
abstract: BACKGROUND
  Acute diarrhea disease is the second cause of death among all infectious diseases in children younger than 5 years of age worldwide. The aim of this study was to employ a combination of biochemical, microbiological and molecular diagnostic techniques to investigate the stools of Iranian children with acute diarrhea for bacterial enteropathogens.
  
  
  METHODS
  Diarrheagenic Escherichia coli, Shigella spp., Salmonella spp., Campylobacter spp. and Yersinia spp., were investigated from June 2003 to June 2005, in 1087 children less than 5 years old with acute diarrhea. Stool specimens from children were studied for enteropathogens both by standard culturing and molecular methods. This study was designed on hospital based.
  
  
  RESULTS
  The highest incidence values were found in the summer and in children less than 1-year-old (42.7%). The Pathogenic bacteria recovered out from fecal samples of 555 (55.1%) patients had the following profile: Shigella spp. (26.7%) was the most prevalent bacterial pathogen and Shiga-like toxin producing E. coli (STEC) and Enteroaggregative E. coli (EAEC) 105 (18.9%) and 92 (16.6%) had the second and third highest prevalence, respectively. Enteropathogenic E. coli (EPEC), Campylobacter, Salmonella and Enterotoxigenic E. coli (ETEC) were found in 70 (12.6%), 60 (10.8%), 42 (7.6%), and 38 (6.8%) positive samples, respectively. In this study neither Yersinia nor E. coli O157:H7 were found. Of the 30 co-infections detected, Shigella flexneri and Campylobacter jejuni accounted for more than 50%.
  
  
  CONCLUSIONS
  Information about the prevalence of wide-range Shigella and STEC may facilitate the control and management of infant diarrhea diseases in Iran. The results of this study suggest that comprehensive surveys are needed in different parts of the country in order to identify the incidence of different enteropathogenic diarrhea, especially diarrheagenic E. coli in children in Iran.
authors.affiliation: The National Research Department of Foodborne and Diarrheal Diseases, Research Center for Gastroenterology and Liver Diseases (RCGLD), Shahid Beheshti University, Taleghani Hospital, Yaman Street, Evin, 19857 Tehran, Iran. jafari.rcgld@gmail.com
authors.forename: F
authors.initials: F
authors.lastname: Jafari
authors.forename: L J
authors.initials: LJ
authors.lastname: Garcia-Gil
authors.forename: S
authors.initials: S
authors.lastname: Salmanzadeh-Ahrabi
authors.forename: L
authors.initials: L
authors.lastname: Shokrzadeh
authors.forename: M M
authors.initials: MM
authors.lastname: Aslani
authors.forename: M A
authors.initials: MA
authors.lastname: Pourhoseingholi
authors.forename: F
authors.initials: F
authors.lastname: Derakhshan
authors.forename: M R
authors.initials: MR
authors.lastname: Zali
mesh_terms: D002169:Campylobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006761:Hospitals; D006801:Humans; D015994:Incidence; D007223:Infant; D007231:Infant, Newborn; D007492:Iran / Q000453:epidemiology; D015995:Prevalence; D012621:Seasons