Assessing the performance of windowed 1H CRAMPS methods, on biological solids, at high-field and MAS up to 35 kHz.
The performance of various high-resolution 1D (1)H CRAMPS pulse schemes at moderate and high static magnetic fields (400 MHz and 800 (1)H Larmor frequencies) and spinning rates up to 35 kHz, using state-of-the-art electronics is compared. The performance of the (1)H windowed acquisition decoupling schemes, wDUMBO, wPMLG3 and wSAM3 is investigated using their effective z-rotation variants on glycine and other small biological molecules, tripeptide reduced glutathione and nucleoside uridine. (1)H CRAMPS spectra, recorded with windowed (1)H-(1)H decoupling methods and fast MAS (35 kHz) and high-field are reported for the first time. (1)H spectra exhibiting outstanding resolution and completely free from any artifact are also shown. The effect on spectra quality of the decoupling rf cycle and rotor periods ratio (tau(C)/tau(R)) and the power requirements needed for each windowed (1)H CRAMPS methods are discussed.
Mafra
Luís
L
Department of Chemistry, CICECO, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. lmafra@ua.pt
Coelho
Cristina
C
Siegel
Renée
R
Rocha
João
J
D000465:Algorithms; D001704:Biopolymers / Q000737:chemistry*; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D011522:Protons
D001704:Biopolymers; D011522:Protons