title: HIV type 1 genetic diversity in Moyale, Mandera, and Turkana based on env-C2-V3 sequences.
abstract: The genetic diversity of HIV-1 subtypes circulating in three districts of northern Kenya, i.e., Turkana, Mandera, and Moyale, was studied. DNA sequences encoding a portion of the env-C2-V3 region of the virus were amplified by PCR and sequenced directly. One hundred and fifty-nine samples were successfully sequenced in the env-C2-V3 region and analyzed. From the analysis, 57% were subtype A1, 27% were subtype C, 9% were subtype D, and the remaining 7% were unclassified. This study showed that HIV-1 subtype A1 was the dominant subtype in circulation in this region, though there was a significant percentage of HIV-1 subtype C in circulation there.
authors.affiliation: Kenya Medical Research Institute, Nairobi, Kenya.
authors.forename: Samoel A
authors.initials: SA
authors.lastname: Khamadi
authors.forename: Raphael W
authors.initials: RW
authors.lastname: Lihana
authors.forename: D L
authors.initials: DL
authors.lastname: Mwaniki
authors.forename: Joyceline
authors.initials: J
authors.lastname: Kinyua
authors.forename: Nancy
authors.initials: N
authors.lastname: Lagat
authors.forename: Jane Y
authors.initials: JY
authors.lastname: Carter
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ichimura
authors.forename: Isao
authors.initials: I
authors.lastname: Oishi
authors.forename: Fredrick A
authors.initials: FA
authors.lastname: Okoth
authors.forename: Washington
authors.initials: W
authors.lastname: Ochieng
mesh_terms: D002648:Child; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus