Highly specific enrichment of glycopeptides using boronic acid-functionalized mesoporous silica.
A novel boronic acid functionalized mesoporous silica, which holds the attractive features of high surface area and large accessible porosity, was developed to enrich glycopeptides. This is the first time that mesoporous material has been introduced into glycoproteome. In comparison to direct (traditional) analysis, this novel method enabled 2 orders of magnitude improvement in the detection limit of glycopeptides. The unbiased nature of organo-boronic acid groups also made this method applicable to all kinds of glycopeptides regardless of their sizes, structures, and hydrophilicities.
Xu
Yawei
Y
Department of Chemistry and Institutes of Biomedical Sciences and Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, P.R. China.
Wu
Zhangxiong
Z
Zhang
Lijuan
L
Lu
Haojie
H
Yang
Pengyuan
P
Webley
Paul A
PA
Zhao
Dongyuan
D
D000595:Amino Acid Sequence; D001897:Boronic Acids / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D053838:Scattering, Small Angle; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014961:X-Ray Diffraction
D001897:Boronic Acids; D006020:Glycopeptides; C028592:3-aminobenzeneboronic acid; D012822:Silicon Dioxide