A simple method to monitor serum concentrations of fluoxetine and its major metabolite for pharmacokinetic studies.
A rapid, selective and sensitive isocratic reversed-phase HPLC assay coupled with MS/MS detection for simultaneous quantification of fluoxetine and its major active metabolite in serum samples has been developed. Analytes were extracted with a simple three step liquid-liquid procedure and chromatographic separation was achieved on a C18 column. Because of its sensitivity, this HPLC/MS/MS method is suitable both for routine therapeutic drug monitoring and for pharmacokinetic studies, due to its low limits of quantification.
Franceschi
Loretta
L
Institute of Clinical Pharmacology and Toxicology, University of Udine, Udine, Italy. loretta.franceschi@uniud.it
Faggiani
Anna
A
Furlanut
Mario
M
D018687:Antidepressive Agents, Second-Generation / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D016903:Drug Monitoring / Q000379:methods; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics*; D006207:Half-Life; D019698:Hepatitis C, Chronic / Q000188:drug therapy; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D018687:Antidepressive Agents, Second-Generation; D005473:Fluoxetine