Neural precursor cell lines promote neurite branching.
Schwann cells and primary progenitor cells improve regeneration across peripheral nerve defects. This study examined the impact of immortalized neural precursor cells on regeneration of rat nerve defects. Across 10-mm gaps, neuromas formed without neural cables with C17.2- or RN33B-transplanted cells, but neural cables formed across 5-mm gaps seeded with RN33B cells. In vitro, dorsal root ganglia neurites elongated across Schwann and RN33B cells; RN33B cells induced neurite branching with shorter total outgrowth. Neural cable formation in vivo was likely determined by the balance of guidance and branch-inducing factors secreted by Schwann and transplanted precursor cells.
Neville
Craig M
CM
Center for Regenerative Medicine, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA.
Huang
Albert Y
AY
Shyu
Jeffrey Y
JY
Snyder
Evan Y
EY
Hadlock
Tessa A
TA
Sundback
Cathryn A
CA
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D020439:Growth Cones / Q000502:physiology* / Q000648:ultrastructure; D007797:Laminin / Q000494:pharmacology; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D055495:Neurogenesis / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology / Q000502:physiology; D011509:Proteoglycans / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D020426:Sciatic Neuropathy / Q000628:therapy; D011984:Sensory Receptor Cells / Q000166:cytology / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen