title: Accurate SHAPE-directed RNA structure determination.
abstract: Almost all RNAs can fold to form extensive base-paired secondary structures. Many of these structures then modulate numerous fundamental elements of gene expression. Deducing these structure-function relationships requires that it be possible to predict RNA secondary structures accurately. However, RNA secondary structure prediction for large RNAs, such that a single predicted structure for a single sequence reliably represents the correct structure, has remained an unsolved problem. Here, we demonstrate that quantitative, nucleotide-resolution information from a SHAPE experiment can be interpreted as a pseudo-free energy change term and used to determine RNA secondary structure with high accuracy. Free energy minimization, by using SHAPE pseudo-free energies, in conjunction with nearest neighbor parameters, predicts the secondary structure of deproteinized Escherichia coli 16S rRNA (>1,300 nt) and a set of smaller RNAs (75-155 nt) with accuracies of up to 96-100%, which are comparable to the best accuracies achievable by comparative sequence analysis.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Deigan
authors.forename: Tian W
authors.initials: TW
authors.lastname: Li
authors.forename: David H
authors.initials: DH
authors.lastname: Mathews
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Weeks
mesh_terms: D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D012984:Software; D013816:Thermodynamics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012313:RNA