title: Mass spectrometry based targeted protein quantification: methods and applications.
abstract: The recent advance in technology for mass spectrometry-based targeted protein quantification has opened new avenues for a broad range of proteomic applications in clinical research. The major breakthroughs are highlighted by the capability of using a "universal" approach to perform quantitative assays for a wide spectrum of proteins with minimum restrictions and the ease of assembling multiplex detections in a single measurement. The quantitative approach relies on the use of synthetic stable isotope labeled peptides or proteins, which precisely mimic their endogenous counterparts and act as internal standards to quantify the corresponding candidate proteins. This report reviews recently developed platform technologies for emerging applications of clinical proteomics and biomarker development.
authors.affiliation: Department of Pathology, University of Washington, Seattle, Washington 98195, USA. span@u.washington.edu
authors.forename: Sheng
authors.initials: S
authors.lastname: Pan
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.forename: Ru
authors.initials: R
authors.lastname: Chen
authors.forename: John
authors.initials: J
authors.lastname: Rush
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: Martin W
authors.initials: MW
authors.lastname: McIntosh
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Brentnall
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D040901:Proteomics / Q000379:methods
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D010455:Peptides