Structural alterations of erythrocyte membrane components induced by exhaustive exercise.
Physical exercise was used as a model of the physiological modulator of free radical production to examine the effects of exercise-induced oxidative modifications on the physico-biochemical properties of erythrocyte membrane. The aim of our work was to investigate conformational changes of erythrocyte membrane proteins, membrane fluidity, and membrane susceptibility to disintegration. Venous blood was taken before, immediately after, and 1 h after an exhaustive incremental cycling test (30 W.min-1 ramp), performed by 11 healthy untrained males on balanced diets (mean age, 22 +/- 2 years; mean body mass index, 25 +/- 4.5 kg.m-2). In response to this exercise, individual maximum heart rate was 195 +/- 12 beats.min-1 and maximum wattage was 292 +/- 27 W. Electron paramagnetic resonance spectroscopy was used to investigate alterations in membrane proteins and membrane dynamics, and to measure production of radical species. The reducing potential of plasma (RPP) was measured using the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric-reducing ability of plasma. Exercise induced decreases in erythrocyte membrane fluidity in the polar region (p < 0.0001) and alterations in the conformational state of membrane proteins (p < 0.05). An increase in RPP was observed immediately after exercise (p < 0.001), with a further increase 1 h postexercise (p < 0.0001). Supporting measurements of lipid peroxidation showed an increase in thiobarbituric acid reactive substances immediately after exercise (p < 0.05) and at 1 h of recovery (p < 0.001); however, free radicals were not detected. Results indicate the existence of early postexercise mild oxidative stress after single-exercise performance, which induced structural changes in erythrocyte membrane components (protein aggregation) and in the membrane organization (lipids rigidization) that followed lipid peroxidation but did not lead to cellular hemolysis.
Brzeszczynska
Joanna
J
School of Life Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, UK. Joanna.Brzeszczynska@hw.ac.uk
Pieniazek
Anna
A
Gwozdzinski
Lukasz
L
Gwozdzinski
Krzysztof
K
Jegier
Anna
A
D000328:Adult; D000704:Analysis of Variance; D001642:Bicycling; D015415:Biomarkers / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004910:Erythrocyte Membrane / Q000502:physiology*; D015444:Exercise / Q000502:physiology*; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology; D008565:Membrane Proteins / Q000097:blood / Q000502:physiology; D009996:Osmotic Fragility / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology; D017382:Reactive Oxygen Species / Q000097:blood; D012016:Reference Values; D013113:Spin Labels; D019097:Spin Trapping / Q000379:methods; D013997:Time Factors
D015415:Biomarkers; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D013113:Spin Labels