title: An incoherent magnetic field inhibited EGF receptor clustering and phosphorylation induced by a 50-Hz magnetic field in cultured FL cells.
abstract: Previously, we found that exposure to a 50-Hz magnetic field (MF) at 0.4 mT could induce epidermal growth factor (EGF) receptor clustering in Chinese hamster lung (CHL) fibroblast cells and superposition of an incoherent MF with the same intensity could inhibit the effect. In the present experiment, we investigated the effects of 50-Hz MF exposure at different intensities on EGF receptor clustering and phosphorylation in human amniotic cells (FL), and explored the interaction effect of an incoherent MF. Clustering and phosphorylation of EGF receptors on cellular membrane surface were analyzed using immunofluorescence assessed by confocal microscopy and western blot technology, respectively. EGF treatment served as a positive control. The results showed that, compared with sham exposure, exposure to a 50-Hz MF at 0.1, 0.2 or 0.4 mT for 15 min could significantly induce EGF receptor clustering and enhance phosphorylation on tyrosine-1173 residue in FL cells, whereas exposure to a 0.05 mT field for 15 min did not caused a significant effect. Exposure to an incoherent MF (frequency range between 30 to 90 Hz) at 0.2 mT for the same time neither induced EGF receptor clustering nor enhanced phosphorylation of EGF receptor in FL cells. When superposed, the incoherent MF at 0.2 mT completely inhibited EGF receptor clustering and phosphorylation induced by a 50-Hz MF at 0.1 and 0.2 mT. However, the incoherent MF could not completely eliminate the effects induced by a 0.4 mT 50-Hz MF. Based on the results of this experiment, we conclude that membrane receptors could be one of the main targets where extremely-low frequency (ELF) MF interacts with cells, and the intensity threshold, in the case of EGF receptors, is between 0.05 and 0.1 mT. An incoherent MF could completely inhibit the effects induced by an ELF-MF of equal or lower intensity.
authors.affiliation: Bioelectromagnetics Laboratory, 2Institute of Environmental Medicine, School of Medicine, Zhejiang University, Hangzhou, China. sunwj@zju.edu.cn
authors.forename: Wenjun
authors.initials: W
authors.lastname: Sun
authors.forename: Yaping
authors.initials: Y
authors.lastname: Gan
authors.forename: Yiti
authors.initials: Y
authors.lastname: Fu
authors.forename: Deqiang
authors.initials: D
authors.lastname: Lu
authors.forename: Huai
authors.initials: H
authors.lastname: Chiang
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology* / Q000201:enzymology*; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism
chemical_list: D019000:Phosphotyrosine; D066246:ErbB Receptors