title: Genetic characterization of orthobunyavirus Melao, strains BE AR633512 and BE AR8033, and experimental infection in golden hamsters (Mesocricetus auratus).
abstract: Melao virus (MELV) strains BE AR8033 and BE AR633512 were isolated from pools of Ochlerotatus scapularis mosquitoes in Belém, Pará State (1955), and Alta Floresta, Rondônia State (2000), Brazil, respectively. The aim of the present study was to molecularly characterize these strains and to describe the histopathological, biochemical and immunological changes in golden hamsters (Mesocricetus auratus) following intraperitoneal injection of MELV strains. Hamsters were susceptible to both of the MELV strains studied. Viraemia was observed 3-6 days post-infection (p.i.) for BE AR633512 and only on the second day p.i. for BE AR8033. Neutralizing antibodies against both strains were detected in blood samples obtained at 5 days p.i. and persisted up to 30 days p.i. Aspartate aminotransferase, alanine aminotransferase and blood urea nitrogen were significantly altered in animals infected with the two MELV strains, while creatinine was only altered in animals inoculated with BE AR633512. Histopathological changes were observed in the central nervous system, liver, kidney and spleen of hamsters, and infection was confirmed by detection of specific MELV antigens by immunohistochemistry. Strain BE AR633512 caused more severe tissue damage than strain BE AR8033, showing increased neurovirulence and pathogenicity. Genetic analysis based on the full-length sequences of the glycoprotein (Gn and Gc) and nucleocapsid protein (N) genes revealed high levels of homology between the MELV strains. Interestingly, the greatest genetic divergence was found for the Gn gene of strain BE AR633512, in which several synonymous and non-synonymous mutations causing changes in RNA secondary structure were observed. Further studies will be necessary to investigate the role of Gn and Gc mutations in the MELV pathogenicity.
authors.affiliation: WHO Collaborating Center for Arbovirus Reference and Research, Department of Arbovirology and Hemorrhagic Fevers at Instituto Evandro Chagas, Ministry of Health, Belém, Pará State, Brazil.
authors.forename: Valéria L
authors.initials: VL
authors.lastname: Carvalho
authors.forename: Márcio R T
authors.initials: MR
authors.lastname: Nunes
authors.forename: Eliana V P
authors.initials: EV
authors.lastname: da Silva
authors.forename: Conceição M A
authors.initials: CM
authors.lastname: Vieira
authors.forename: Manoel
authors.initials: M
authors.lastname: Gomes
authors.forename: Samir M
authors.initials: SM
authors.lastname: Casseb
authors.forename: Sueli G
authors.initials: SG
authors.lastname: Rodrigues
authors.forename: Joaquim P
authors.initials: JP
authors.lastname: Nunes-Neto
authors.forename: Juarez A O
authors.initials: JA
authors.lastname: Quaresma
authors.forename: Pedro F C
authors.initials: PF
authors.lastname: Vasconcelos
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001938:Brazil; D002044:Bunyaviridae Infections / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology* / Q000821:virology; D002490:Central Nervous System / Q000473:pathology; D003404:Creatinine / Q000097:blood; D006224:Cricetinae; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008647:Mesocricetus / Q000821:virology*; D008958:Models, Molecular; D009500:Neutralization Tests; D009690:Nucleic Acid Conformation; D016851:Orthobunyavirus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000473:pathology; D014508:Urea / Q000097:blood; D014766:Viremia
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D014508:Urea; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase