[Promotive effect of adipose-derived stem cells on the wound model of human epidermal keratinocytes in vitro].
OBJECTIVE
To investigate the migrating effect of adipose derived stem cells (ADSCs) on the wound model of human epidermal keratinocyte (HEKa).


METHODS
Rat ADSCs (rADSCs) were isolated and cultured (n = 10), rADSCs were direct co-cultured with HEKa cells in experiment group (experimental group, n = 10). In the control groups, rADSCs were indirect co-cultured with HEKa cells in transwell chamber (indirect group, n = 8), or HEKa was cultured alone (single group, n = 8). Then confluent HEKa cells were scraped to establish a wound model under invert microscope. After scraped 24, 48, and 72 h, cell numbers of which migrated across the edge of the wound was measured, the rate of wound healing was calculated by using SigmaScan Pro 5 software, and the proliferating effect of rADSCs on HEKa were examined by incorporation of [(3)H] thymidine.


RESULTS
The cells migrated across the edge of wound after 24 hours in experimental group, indirect group, and single group were (9.2 + or - 0.2), (5.0 + or - 0.3), (4.2 + or - 0.3), and were (58.5 + or - 0.4), (26.5 + or - 0.3), (20.7 + or - 0.5) 48 hours after, and were (125.8 + or - 0.4), (43.0 + or - 0.5), (35.6 + or - 0.5) cells/HP 72 hours after, respectively; the numbers were all significantly higher in experimental group than those in control groups (P < 0.05). The rates of wound healing after scraped 72 hours were 61.0% + or - 3.0%, 35.0% + or - 2.5% and 32.0 + or - 2.1%, the outcome in experimental group was significantly better than in the control groups (P < 0.05). And the thymidine feeding displayed the proliferation of HEKa in the three groups were (1440 + or - 210), (1050 + or - 280) and (1130 + or - 390) cpm/10(5) cell, and there was significant difference between the experimental and the control groups (P < 0.05).


CONCLUSIONS
The rADSCs can promote the migration of HEKa by direct contact with it.
YUAN
Fang
F
Health and Science Center, Cardiovascular Institute, Peking University, Beijing 100038, China.
LEI
Yong-hong
YH
FU
Xiao-bing
XB
SHENG
Zhi-yong
ZY
CAI
Sa
S
SUN
Tong-zhu
TZ
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D014945:Wound Healing