[Experimental study of electroporation-mediated plasmid gene expression in skin and incisional wound].
OBJECTIVE
To explore the feasibility of electroporation mediated gene transfer in rat incisional wound.


METHODS
12 Sprague-Dawley rat's dorsal skins were electroporated (800 voltages in amplitude with 6 square wave pulses, each lasting 20 milliseconds with 200 millisecond interval) after injection of plasmid DNA (1 microg/microl, in 100 microl PBS) containing enhanced green fluorescence protein (EGFP) gene. Electroporated skins were incisionally wounded 24 hours after electroporation. Specimens were harvested at day 2, 4, 6, 14, then EGFP expression in dermis was observed and quantitatively analyzed with integrated optical density (IOD) followed by H&E staining.


RESULTS
Electroporation can mediate EGFP expression in epidermis, dermis and panniculus muscle. The expression level in dermis was the highest at day 2 (IOD = 3.50 +/- 1.45) and disappeared at day 14. EGFP expression was not found in dermis if no electroporation applied after plasmid injection (IOD = 0).


CONCLUSIONS
Electroporation can mediate plasmid gene expression in incisional wound efficiently and widely.
Gao
Zhen
Z
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
Song
Nan
N
Wu
Xiao-Li
XL
Cao
Yi-Lin
YL
Liu
Wei
W
D000818:Animals; D018274:Electroporation; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D014162:Transfection
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins