title: Survival and differentiation of neuroepithelial stem cells following transplantation into the lateral ventricle of rats.
abstract: Neuroepithelial stem cells (NEPs) demonstrate a high potential for self-renewal and differentiation during embryonic development. To explore the survival and differentiation of NEPs in vivo, we isolated NEPs from green fluorescence protein (GFP) transgenic embryos and transplanted into the lateral ventricle of rats. In vitro culture, NEPs proliferated into neurospheres and differentiated into both neurons and glia. When transplanted into the lateral ventricle of rats, these GFP positive NEPs (GFP+ NEPs) survived and attached to the wall of ventricle. Moreover, grafted cells differentiated into neuron-specific enolase (NSE) positive neurons and glial fibrillary acidic protein (GFAP) positive astrocytes and migrated into the host brain. Thus, our results indicate that NEPs can survive and differentiate into neurons and astrocytes in the lateral ventricle following transplantation.
authors.affiliation: Institute of Anatomy and Histology & Embryology, School of Medicine, Shandong University, Jinan 250012, People's Repulic of China. sunjinhao@sdu.edu.cn
authors.forename: Jin-Hao
authors.initials: JH
authors.lastname: Sun
authors.forename: Pei-Hui
authors.initials: PH
authors.lastname: Zheng
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Wang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Li
authors.forename: Jia-Zeng
authors.initials: JZ
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Liu
authors.forename: Ying-Mao
authors.initials: YM
authors.lastname: Gao
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000166:cytology*; D046569:Neuroepithelial Cells / Q000166:cytology* / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins; D010751:Phosphopyruvate Hydratase