title: Effect of Urtica dioica on morphometric indices of kidney in streptozotocin diabetic rats--a stereological study.
abstract: The aim of the present study was to investigate the effect of Urtica dioica on Morphometric indices of kidney in diabetic rats. Thirty male adult albino wistar rats of 125-175 g divided into control, diabetic and Urtica dioica treatment groups. In treatment Group, diabetic rats received 100 mg kg(-1) daily hydroalcoholic extract of U. dioica intraperitoneally for 4 weeks. After the animals had been sacrified, the kidneys were removed and fixed by formaldehyde, cut horizontally into 1 mm slices and processed, Stained with H and E. Stereological study performed using light microscope and the image projected on a table of olysa software. Cavalieri principle was used to estimate the volume of cortex, medulla and whole kidney. All the grouped data statistically evaluated using Student's t-test, expressed as the Mean +/- SE. Ration of kidney weight/body weight in diabetes (0.51) and diabetes-extract group (0.67) were higher then control group (0.42). Ratio of kidney volume/body weight in diabetes (350) and diabetes-extract group (348) were higher then control group (323). Volume Ratio of cortex/medulla in diabetes-extract group (1.65) was higher then control (1.34) and diabetes group (1.33). Glomerular area and diameter and proximal tubule diameter in diabetes-Extract group was higher than control and diabetes groups. This study revealed that Urtica dioica has no effect on renal morphometric indices in induced diabetic rats.
authors.affiliation: Department of Embryology and Histology, Gorgan Congenital Malformations Research Center, Gorgan University of Medical Sciences, P.O. Box 14165-553, Iran.
authors.forename: Mohammad Jafar
authors.initials: MJ
authors.lastname: Golalipour
authors.forename: Anneh Mohammad
authors.initials: AM
authors.lastname: Gharravi
authors.forename: Sorya
authors.initials: S
authors.lastname: Ghafari
authors.forename: Mohammad
authors.initials: M
authors.lastname: Afshar
mesh_terms: D000818:Animals; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D015233:Models, Statistical; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D026982:Urtica dioica / Q000378:metabolism*
chemical_list: D010936:Plant Extracts