An informative set of SSLP markers and genomic profiles in the rat MHC, the RT1 complex.
Almost 10,000 single nucleotide polymorphisms (SNPs) had been identified in the RT1 complex, the major histocompatibility complex of the rat, but less than approximately 0.5% have been characterized. In the context of the incomplete characterization of most SNPs, simple sequence length polymorphism (SSLP) marker development is still valuable for understanding the involvement of genes in the RT1 in controlling disease susceptibility, since SSLPs are user-friendly and cost-effective genetic markers in rat genome analysis. In this study, we developed a set of 67 SSLP markers, including 57 novel markers, to cover the entire RT1 complex and then created genetic profiles across 67 rat strains. These markers are located almost every 50 kb in the RT1 complex and show comparable polymorphism; the average number of alleles was 8.04 +/- 3.44 and the average polymorphic rate was 71 +/- 23%. Interestingly, markers failing to amplify polymerase chain reaction products were highly observed in all strains except for BN/SsNHsd, which suggests the existence of highly variable genomic sequences or genomic rearrangements in the RT1 region across rat strains. Based on the phylogenic tree and individual genotyping data, we identified 28 SSLP marker haplotypes in the RT1 region that roughly consisted of three genomic regions. These findings provided new insight into the genomic organization of the RT1 complex and we recognized the need of additional RT1 genome sequences in different strains. Owing to the accuracy and ease of determination, PCR-based SSLP genotyping could replace serological typing in genetic analyses and characterization of rat major histocompatibility.
Takagi
Yumie
Y
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho Sakyo-ku, Kyoto 606-8501, Japan.
Kuramoto
Takashi
T
Voigt
Birger
B
Tsurumi
Toshiko
T
Nakanishi
Satoshi
S
Mashimo
Tomoji
T
Masui
Norio
N
Serikawa
Tadao
T
D000818:Animals; D006649:Histocompatibility Antigens / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051381:Rats / Q000235:genetics*
D006649:Histocompatibility Antigens; C038353:histocompatibility antigens RT, rat