Cloning and characterization of a novel CBL-interacting protein kinase from maize.
A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ZmCIPK16 interacted with ZmCBL3, ZmCBL4, ZmCBL5, and ZmCBL8. Bimolecular fluorescence complementation (BiFC) assays prove that ZmCIPK16 can interact with ZmCBL3, ZmCBL4, ZmCBL5, and ZmCBL8 in vivo. Subcellular localization showed that ZmCIPK16 is distributed in the nucleus, plasma membrane and cytoplasm; this is different from the specific localization of ZmCBL3, ZmCBL4, and ZmCBL5, which are found in the plasma membrane. The results also showed that overexpression of ZmCIPK16 in the Arabidopsis sos2 mutant induced the expression of the SOS1 gene and enhanced salt tolerance. These findings indicate that ZmCIPK16 may be involved in the CBL-CIPK signaling network in maize responses to salt stress.
Zhao
Jinfeng
J
State Key Laboratory of Agrobiotechnology and Department of Seed Science and Technology, China Agricultural University, Beijing 100094, China.
Sun
Zhenfei
Z
Zheng
Jun
J
Guo
Xiying
X
Dong
Zhigang
Z
Huai
Junling
J
Gou
Mingyue
M
He
Junguang
J
Jin
Yongsheng
Y
Wang
Jianhua
J
Wang
Guoying
G
D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009154:Mutation; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011092:Polyethylene Glycols / Q000494:pharmacology; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics; D020798:Two-Hybrid System Techniques; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; C477653:SOS1 protein, Arabidopsis; D017923:Sodium-Hydrogen Exchangers; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D000040:Abscisic Acid; D011494:Protein Kinases; C406738:SOS2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases