title: Determining in vivo activity of the yeast cytoplasmic exosome.
abstract: A 3'-exoribonuclease complex, termed the exosome, has important functions in the cytoplasm, as well as in the nucleus, and is involved in 3'-processing and/or decay of many RNAs. This chapter will discuss methods to study cytoplasmic exosome function in yeast with in vivo approaches. The first section will describe mutants that are available to study the processing or decay of a specific RNA by the nuclear or cytoplasmic exosome. The second section will discuss methods to determine whether the cytoplasmic exosome is functional under a specific condition(s) with reporter mRNAs that are known substrates of this complex.
authors.affiliation: University of Texas Health Science Center-Houston, Department of Microbiology and Molecular Genetics, Houston, Texas, USA.
authors.forename: Daneen
authors.initials: D
authors.lastname: Schaeffer
authors.forename: Stacie
authors.initials: S
authors.lastname: Meaux
authors.forename: Amanda
authors.initials: A
authors.lastname: Clark
authors.forename: Ambro
authors.initials: A
authors.lastname: van Hoof
mesh_terms: D005095:Exoribonucleases / Q000032:analysis* / Q000378:metabolism*; D055354:Exosomes / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009154:Mutation / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D012313:RNA; D005095:Exoribonucleases