title: The expression of the novel CYP2W1 enzyme is an independent prognostic factor in colorectal cancer - a pilot study.
abstract: OBJECTIVE
  Cytochrome P450 (CYP) enzymes are important for drug metabolism. A novel cytochrome P450 enzyme, CYP2W1, has recently been identified. This enzyme is mainly found in foetal colon tissue and in tumour tissue. In this pilot study, we have investigated the expression of CYP2W1 in 162 tumours from patients with stages II and III colorectal cancer.
  
  
  METHODS
  The expression of CYP2W1 enzyme was immunohistochemically detected using a polyclonal antibody. Staining intensity was defined using a visual grading scale from 0 to 3. Grades 0-2 were classified as low, and grade 3 was classified as high expression of CYP2W1.
  
  
  RESULTS
  About 64% of the tumours expressed a low level of CYP2W1-expression, and 36% expressed a high level. CYP2W1-expression was an independent prognostic factor for overall survival (p=0.007), where a high expression was associated with a worse clinical outcome.
  
  
  CONCLUSIONS
  Immunohistochemically assessed expression of CYP2W1 is an independent prognostic factor in patients with stages II and III colorectal cancer.
authors.affiliation: Department of Surgery, P903, Karolinska University Hospital Solna, S 171 76 Stockholm, Sweden. david.edler@telia.com
authors.forename: David
authors.initials: D
authors.lastname: Edler
authors.forename: Kristina
authors.initials: K
authors.lastname: Stenstedt
authors.forename: Katarina
authors.initials: K
authors.lastname: Ohrling
authors.forename: Marja
authors.initials: M
authors.lastname: Hallström
authors.forename: Maria
authors.initials: M
authors.lastname: Karlgren
authors.forename: Magnus
authors.initials: M
authors.lastname: Ingelman-Sundberg
authors.forename: Peter
authors.initials: P
authors.lastname: Ragnhammar
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D000072467:Cytochrome P450 Family 2; D005260:Female; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D009367:Neoplasm Staging; D010865:Pilot Projects; D011379:Prognosis; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D003577:Cytochrome P-450 Enzyme System; C509336:CYP2W1 protein, human; D000072467:Cytochrome P450 Family 2