Polymorphisms of p53, GSTM1 and GSTT1, and HPV in uterine cervix adenocarcinoma.
OBJECTIVE
To analyze the participation of glutathione-S-transferase (GST) M1 and T1 polymorphisms associated or not with protein p53 polymorphism at codon 72 and in the presence of HPV in the carcinogenesis of uterine cervix adenocarcinoma.


METHODS
Forty-three samples of uterine cervix adenocarcinoma were studied and 86 samples of endocervical cells of women without tumors formed the control group. The presence of HPV was determined in order to genotype the isoforms of p53 at codon 72, GSTM1, GSTM1*0, GSTT1 and GSTT1*0 which were evaluated by the PCR method.


RESULTS
HPV was present in 97.67% of the adenocarcinoma cases and in 31.40% of the control group. Statistical analysis showed differences (p = 0.001) and an OR of 113.3 (CI 95%: 13.67-947.14). GSTT1 and GSTT1*0 analysis showed a significant difference between the groups (p = 0.001) with an OR of 4.58 (CI 95%: 2.041-10.28) (p < 0.001) for the presence of GSTT1*0. When it was associated with HPV OR was 6.6 (CI 95%: 0.04-0.50). Analyses of p53 and GSTM1 and GSTM1*0 either alone or associated with HPV were not significant.


CONCLUSIONS
The presence of GSTT1*0 increased the risk for uterine cervix adenocarcinoma development while the allele GSTT1 had a protective action. The other isoforms did not appear to participate in the carcinogenesis of uterine cervix adenocarcinoma.
de Carvalho
C R Nogueira
CR
Department of Gynecology, Universidade Federal de São Paulo/Escola Paulista de Medicina, Brazil. carmenrcarvalho@uol.com.br
da Silva
I D C Guerreiro
ID
Pereira
J S
JS
de Souza
N C Nogueira
NC
Focchi
G Rubino de Azevedo
GR
Ribalta
J C L
JC
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000302:isolation & purification*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D016158:Genes, p53 / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*; D055815:Young Adult
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1