title: Antagonists of the protein kinase A and mitogen-activated protein kinase systems and of the progestin receptor block the ability of vaginocervical/flank-perineal stimulation to induce female rat sexual behaviour.
abstract: Brief vaginocervical stimulation using a glass rod (VCS) combined with manual flank-perineal stimulation (FS) rapidly (within 5 min) induced both receptive and proceptive behavioural responses to males in ovariectomised, oestrogen-primed rats. This receptive-proceptive response to males, resulting from a single brief (5-s duration) instance of manual VCS + FS, declined markedly within 4 h. However, the decline was prevented if the females were mounted by males immediately after the manual VCS + FS and 2 h later. We tested the participation of the cAMP-dependent protein kinase A system and the mitogen-activated protein kinase (MAPK) system in the response to VCS + FS by infusing either 100 ng of Rp-adenosine 3',5'-cyclic monophosphorothiate triethylamonium salt (a protein kinase A blocker) or 3.3 microg of PD98059 (a MAPK blocker) i.c.v. 15 min prior to VCS + FS. Both inhibitors blocked the ability of VCS + FS to induce the proceptive-receptive responses to males at all testing intervals. In experiment 2, systemic administration of 5 mg of RU486 1 h before VCS + FS also blocked the ability of VCS + FS to induce the proceptive-receptive responses to males. The present findings suggest that both VCS + FS and mating stimuli provided by males release neurotransmitters and neuromodulators that trigger the protein kinase A and the MAPK signalling systems, which interact with the progestin receptor to rapidly (within 5 min) induce proceptive-receptive behaviour in females.
authors.affiliation: Centro de Investigación en Reproducción Animal, CINVESTAV-Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico. oglezflo@hotmail.com
authors.forename: O
authors.initials: O
authors.lastname: González-Flores
authors.forename: A M
authors.initials: AM
authors.lastname: Etgen
authors.forename: B K
authors.initials: BK
authors.lastname: Komisaruk
authors.forename: P
authors.initials: P
authors.lastname: Gómora-Arrati
authors.forename: A
authors.initials: A
authors.lastname: Macias-Jimenez
authors.forename: F J
authors.initials: FJ
authors.lastname: Lima-Hernández
authors.forename: M
authors.initials: M
authors.lastname: Garcia-Juárez
authors.forename: C
authors.initials: C
authors.lastname: Beyer
mesh_terms: D000818:Animals; D002584:Cervix Uteri / Q000502:physiology; D003283:Contraceptives, Postcoital, Synthetic / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010052:Ovariectomy; D010502:Perineum / Q000502:physiology; D011187:Posture; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D013873:Thionucleotides / Q000494:pharmacology; D014621:Vagina / Q000502:physiology
chemical_list: D003283:Contraceptives, Postcoital, Synthetic; D005419:Flavonoids; D047428:Protein Kinase Inhibitors; D011980:Receptors, Progesterone; D013873:Thionucleotides; C074283:estradiol 3-benzoate; C016957:adenosine-3',5'-cyclic phosphorothioate; D015735:Mifepristone; D004958:Estradiol; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one