[Role of renin-angiotensin system, PPARalpha, PPARgamma in the development of alcoholic cardiomyopathy].
OBJECTIVE
To explore the time course of renin-angiotensin system (RAS), peroxisome proliferator-activated receptor (PPAR) alpha and PPARgamma change in an animal model of alcoholic cardiomyopathy (ACM).


METHODS
Adult rats were divided into three groups: ACM group (A, 10% alcohol as drinking water plus 5 ml 60% alcohol.kg(-1).d(-1) per gavage in the 1st week; 10% alcohol as drinking water plus 10 ml 60% alcohol/kg bid per gavage in the 2nd week, 20% alcohol as drinking water plus 15 ml 60% alcohol/kg bid per gavage during week 3 to week 16), ACM/ARB group(B, A + Irbesartan 5 mg.kg(-1).d(-1) per gavage) and control group (C). mRNA expressions and activities of renin, angiotensin, ACE and AT1 were detected with RT-PCR and radioimmunoassay methods. Protein expressions of PPARalpha and PPARgamma were determined with Western blot. Echocardiogram, optic (HE) and electron microscope examinations were also performed. Parameters were obtained at 2 or 6 months (n = 7 - 10 each).


RESULTS
Compared with group C, LV/BW ratio was significantly increased and LVEF significantly decreased, activities and expressions of Ang I, Ang II and renin were gradually increased, protein expressions of PPARalpha were gradually decreased at 2 and 6 months in group A (all P < 0.05). These changes could be partly attenuated by Irbesartan.


CONCLUSIONS
Activated RAS and decreased protein expressions of PPARalpha and PPARgamma contributed to the development of ACM.
Li
Wei-min
WM
First Clinical Hospital of Harbin Medical University, Harbin 150001, China.
Jing
Ling
L
Song
Jian
J
Zhou
Li-jun
LJ
D000818:Animals; D002310:Cardiomyopathy, Alcoholic / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D047493:PPAR alpha / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012084:Renin-Angiotensin System
D047493:PPAR alpha; D047495:PPAR gamma