Small animal models of hepatocyte transplantation.
In this chapter, we describe techniques used to determine the efficiency of hepatocyte transplantation in animal models of liver disease. We have included the Gunn rat as a model of an inherited liver disease without hepatocyte damage and Abcb4 knockout mice as a model for an inherited liver disease with hepatocyte damage. Immunodeficient mice are included as an animal model for human hepatocyte transplantation.We describe problems that can be encountered in the maintenance and breeding of Gunn rats and immunodeficient Rag2/gamma common knockout mice. Protocols for the collection of bile in rats and mice are described, and we have also detailed the detection of green fluorescent protein (GFP)-labelled human hepatocytes in immunodeficient mice in this chapter.
Seppen
Jurgen
J
AMC Liver Center, Amsterdam, The Netherlands.
Filali
Ebtisam El
EE
Elferink
Ronald Oude
RO
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000821:Animal Feed; D000818:Animals; D001646:Bile / Q000378:metabolism; D003414:Crigler-Najjar Syndrome / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D004268:DNA-Binding Proteins / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D016031:Liver Transplantation / Q000379:methods*; D051379:Mice; D018345:Mice, Knockout; D023421:Models, Animal; D051381:Rats; D011911:Rats, Gunn; D000097202:ATP-Binding Cassette Sub-Family B Member 4
D018435:ATP Binding Cassette Transporter, Subfamily B; D004268:DNA-Binding Proteins; D049452:Green Fluorescent Proteins; D053631:Interleukin Receptor Common gamma Subunit; D000097202:ATP-Binding Cassette Sub-Family B Member 4; C499308:Rag2 protein, mouse