[Shengjing granule: an effective Chinese medicine for spermatogenic disturbance in mice].
OBJECTIVE
To observe the effect of the Chinese medicine Shengjing Granule on spermatogenic disturbance in mice.


METHODS
Forty-six male Kunming mice were randomly divided into a normal (n = 10), a model (n = 12), a control (n = 12) and a Shengjing group (n = 12), and models of spermatogenic disturbance were established by intraperitoneal injection of cyclophosphamide in the latter three. Then the first two groups received intragastric administration of physiological saline and the second two that of clomiphene (21.6 mg/kg x d) and Shengjing Granule (16 g/kg x d), respectively, all for 15 days. On the following day, the testis weight was measured, the levels of the follicle stimulating hormone (FSH), the luteinizing hormone (LH) and testosterone (T) determined by radioimmunoassay. Histological observations were made of the testis tissues under the microscope.


RESULTS
The testis index and T level were (3.958 +/- 0.342) g/kg and (7.046 +/- 0.291) nmo/L, obviously increased, while the levels of FSH and LH were (2.947 +/- 0.587) mIU/ml and (3.254 +/- 0.492) mIU/ml, significantly decreased, in the Shengjing group, as compared with (3.525 +/- 0.462) g/kg, (6.231 +/- 0.317) nmol/L, (5.428 +/- 0.719) mIU/ml and (5.155 +/- 0.460) mIU/ml in the model group (P < 0.05). The number of spermatogenic cells on the seminiferous tubules and that of sperm in the lumina of the tubules were markedly increased in the Shenginjg group, compared with the models. CoCONCLUSIONShenginjg Granule is effective for spermatogenic disturbance in mice.
Wang
Rui
R
Department of Urology, The First Hospital Affiliated to Zhengzhou University/Open Laboratory for Clinical Medicine, Key Discipline of Henan Higher Education, Zhengzhou, Henan 450052, China. huzi918@hotmail.com
Xu
Lei
L
Zhang
Wei-Xing
WX
Wu
Yu-Feng
YF
Shi
Jian-Hua
JH
Zhang
Yue-Xi
YX
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007248:Infertility, Male / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D013091:Spermatogenesis
D004365:Drugs, Chinese Herbal