title: Neural precursor cell lines promote neurite branching.
abstract: Schwann cells and primary progenitor cells improve regeneration across peripheral nerve defects. This study examined the impact of immortalized neural precursor cells on regeneration of rat nerve defects. Across 10-mm gaps, neuromas formed without neural cables with C17.2- or RN33B-transplanted cells, but neural cables formed across 5-mm gaps seeded with RN33B cells. In vitro, dorsal root ganglia neurites elongated across Schwann and RN33B cells; RN33B cells induced neurite branching with shorter total outgrowth. Neural cable formation in vivo was likely determined by the balance of guidance and branch-inducing factors secreted by Schwann and transplanted precursor cells.
authors.affiliation: Center for Regenerative Medicine, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA.
authors.forename: Craig M
authors.initials: CM
authors.lastname: Neville
authors.forename: Albert Y
authors.initials: AY
authors.lastname: Huang
authors.forename: Jeffrey Y
authors.initials: JY
authors.lastname: Shyu
authors.forename: Evan Y
authors.initials: EY
authors.lastname: Snyder
authors.forename: Tessa A
authors.initials: TA
authors.lastname: Hadlock
authors.forename: Cathryn A
authors.initials: CA
authors.lastname: Sundback
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D020439:Growth Cones / Q000502:physiology* / Q000648:ultrastructure; D007797:Laminin / Q000494:pharmacology; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D055495:Neurogenesis / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology / Q000502:physiology; D011509:Proteoglycans / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology* / Q000637:transplantation*; D020426:Sciatic Neuropathy / Q000628:therapy; D011984:Sensory Receptor Cells / Q000166:cytology / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen