title: Design and practices for use of automated drilling and sample handling in MARTE while minimizing terrestrial and cross contamination.
abstract: Mars Astrobiology Research and Technology Experiment (MARTE) investigators used an automated drill and sample processing hardware to detect and categorize life-forms found in subsurface rock at Río Tinto, Spain. For the science to be successful, it was necessary for the biomass from other sources--whether from previously processed samples (cross contamination) or the terrestrial environment (forward contamination)-to be insignificant. The hardware and practices used in MARTE were designed around this problem. Here, we describe some of the design issues that were faced and classify them into problems that are unique to terrestrial tests versus problems that would also exist for a system that was flown to Mars. Assessment of the biomass at various stages in the sample handling process revealed mixed results; the instrument design seemed to minimize cross contamination, but contamination from the surrounding environment sometimes made its way onto the surface of samples. Techniques used during the MARTE Río Tinto project, such as facing the sample, appear to remove this environmental contamination without introducing significant cross contamination from previous samples.
authors.affiliation: University of Oklahoma, Norman, Oklahoma 73019, USA. dpmiller@ou.edu
authors.forename: David P
authors.initials: DP
authors.lastname: Miller
authors.forename: Rosalba
authors.initials: R
authors.lastname: Bonaccorsi
authors.forename: Kiel
authors.initials: K
authors.lastname: Davis
mesh_terms: D000255:Adenosine Triphosphate; D001331:Automation; D004785:Environmental Pollutants; D018559:Exobiology / Q000295:instrumentation / Q000379:methods*; D019015:Geologic Sediments; D008163:Luminescent Measurements; D018535:Mars; D012106:Research; D012371:Robotics / Q000295:instrumentation; D018561:Space Simulation; D018531:Spacecraft; D013030:Spain; D013672:Technology
chemical_list: D004785:Environmental Pollutants; D000255:Adenosine Triphosphate