title: Sialic acid dependence in rotavirus host cell invasion.
abstract: We used NMR spectroscopy, molecular modeling and infectivity competition assays to investigate the key interactions between the spike protein (VP8(*)) from 'sialidase-insensitive' human Wa and 'sialidase-sensitive' porcine CRW-8 rotaviruses and the glycans of gangliosides G(M1) and G(D1a). Our data provide strong evidence that N-acetylneuraminic acid is a key determinant for binding of these rotaviruses. This is in contrast to the widely accepted paradigm that sialic acids are irrelevant in host cell recognition by sialidase-insensitive rotaviruses.
authors.affiliation: Institute for Glycomics, Gold Coast Campus, Griffith University, Parklands Drive, Southport, Queensland 4222, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselhorst
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Fleming
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Dyason
authors.forename: Regan D
authors.initials: RD
authors.lastname: Hartnell
authors.forename: Xing
authors.initials: X
authors.lastname: Yu
authors.forename: Gavan
authors.initials: G
authors.lastname: Holloway
authors.forename: Kim
authors.initials: K
authors.lastname: Santegoets
authors.forename: Milton J
authors.initials: MJ
authors.lastname: Kiefel
authors.forename: Helen
authors.initials: H
authors.lastname: Blanchard
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Coulson
authors.forename: Mark
authors.initials: M
authors.lastname: von Itzstein
mesh_terms: D009682:Magnetic Resonance Spectroscopy; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D012401:Rotavirus / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D019158:N-Acetylneuraminic Acid