Lowered expressions of the NF-kappaB family members in dendritic cells from NOD mice are associated with a reduced expression of GATA-2.
In type 1 diabetes, dendritic cells (DCs) display defective phenotype and function and possibly play crucial roles in the pathogenesis of this disease. In the present study, we compared transcription profiles of CD11c(+) bone marrow (BM)-derived DCs from NOD mice with those from NON mice, focusing on the NF-kappaB/Rel family members and associated molecules. The BMDCs from NOD mice displayed reduced mRNA expressions of NF-kappaB components, p65, p50, p52, and RelB, compared to NON mice: the proportions of each molecule relative to those of NON DCs were 53.9, 54.1, 54.0, and 37.0%, respectively, which were accompanied with lowered expressions of downstream immunomodulatory molecules, including IL-6, CD80, CD86, 4-1BB, and CD40. The reduction of NF-kappaB components possibly underlies the defective phenotype and function of DCs from NOD mice, and could predispose to autoimmunity.
Takahashi
Kazuma
K
Department of Diabetes and Metabolism, Iwate Medical University, Morioka, Japan. kazumat@iwate-med.ac.jp
Satoh
Jo
J
Oka
Yoshitomo
Y
D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology; D015536:Down-Regulation; D050989:GATA2 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D051379:Mice; D016688:Mice, Inbred NOD; D005810:Multigene Family / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse; D016328:NF-kappa B