Detection of Rickettsia spp. in Danish ticks (Acari: Ixodes ricinus) using real-time PCR.
We examined 192 Ixodes ricinus tick nymphs from Denmark for the presence of Rickettsia spp. We used a species-specific real-time PCR; 13.0% of the ticks were positive for rickettsial DNA. Only DNA from Rickettsia helvetica was found in the ticks.
Svendsen
Claus Bo
CB
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. clv@ssi.dk
Krogfelt
Karen A
KA
Jensen
Per Moestrup
PM
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003718:Denmark; D018884:Ixodes / Q000254:growth & development / Q000382:microbiology*; D009758:Nymph / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013045:Species Specificity; D014197:Trees
D004269:DNA, Bacterial