title: True ovarian volume is underestimated by two-dimensional transvaginal ultrasound measurement.
abstract: OBJECTIVE
  To investigate a possible difference between true ovarian volume and ovarian volume estimated with two-dimensional (2D) transvaginal ultrasound.
  
  
  METHODS
  Prospective clinical and laboratory study.
  
  
  METHODS
  University hospital research laboratory.
  
  
  METHODS
  Premenopausal girls and women from three Danish national fertility centers (A: n = 42; B: n = 6; C: n = 18), who had one entire ovary removed for cryopreservation of ovarian cortex.
  
  
  METHODS
  Transvaginal 2D ultrasound measurement of ovarian volume before oophorectomy. True ovarian volume was obtained by weighing the ovary.
  
  
  METHODS
  Ovarian volume estimated by weight and ultrasound.
  
  
  RESULTS
  Ovarian tissue density was 1.00 g/mL. Mean ovarian volume by ultrasound vs. weight in the three groups was as follows: A: 6.3 mL vs. 7.8 mL; B: 5.4 mL vs. 6.8 mL; and C: 2.8 mL vs. 6.1 ml. Ovarian volume obtained by ultrasound was at least 27% smaller than the true ovarian volume.
  
  
  CONCLUSIONS
  Ovarian volume was severely underestimated by transvaginal 2D ultrasound measurement.
authors.affiliation: Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark. mikkel.rosendahl@rh.regionh.dk
authors.forename: Mikkel
authors.initials: M
authors.lastname: Rosendahl
authors.forename: Erik
authors.initials: E
authors.lastname: Ernst
authors.forename: Per Emil
authors.initials: PE
authors.lastname: Rasmussen
authors.forename: Claus Yding
authors.initials: CY
authors.lastname: Andersen
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000009:adverse effects; D007247:Infertility, Female / Q000209:etiology; D008558:Melphalan / Q000009:adverse effects; D008597:Menstrual Cycle; D009369:Neoplasms / Q000150:complications / Q000188:drug therapy; D009865:Oocytes / Q000166:cytology / Q000000981:diagnostic imaging; D010052:Ovariectomy; D010053:Ovary / Q000166:cytology* / Q000000981:diagnostic imaging*; D011446:Prospective Studies; D014463:Ultrasonography / Q000379:methods*; D014621:Vagina
chemical_list: D007074:Immunoglobulin G; C051713:antineoplastic agent K 18; D054304:Anti-Mullerian Hormone; D008558:Melphalan