title: Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo.
abstract: Understanding the diverse activities of the multisubunit core promoter recognition complex TFIID in vivo requires knowledge of how individual subunits contribute to overall functions of this TATA box-binding protein (TBP)/TBP-associated factor (TAF) complex. By generating altered holo-TFIID complexes in Drosophila we identify the ETO domain of TAF4 as a coactivator domain likely targeted by Pygopus, a protein that is required for Wingless-induced transcription of naked cuticle. These results establish a coactivator function of TAF4 and provide a strategy to dissect mechanisms of TFIID function in vivo.
authors.affiliation: Howard Hughes Medical Institute, Li Ka-Shing Center for Biomedical and Sciences, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Wright
authors.forename: Robert
authors.initials: R
authors.lastname: Tjian
mesh_terms: D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007814:Larva; D046912:Multiprotein Complexes; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D015398:Signal Transduction; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000378:metabolism*; D014157:Transcription Factors; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046912:Multiprotein Complexes; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; C082222:RUNX1T1 protein, human; C101882:TAF4 protein, human; D035381:TATA-Binding Protein Associated Factors; C103338:Taf4 protein, Drosophila; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D051153:Wnt Proteins; C453400:pygo protein, Drosophila