title: Increased oxidative damage in peripheral blood correlates with severity of Parkinson's disease.
abstract: Increased oxidative stress contributes to neuronal dysfunction in Parkinson's disease (PD). We investigated whether the pathological changes in PD brains may also be present in peripheral tissues. Leukocyte 8-hydroxydeoxyguanosine (8-OHdG), plasma malondialdehyde (MDA), erythrocyte glutathione peroxidase (GPx) and plasma vitamin E (Vit E) were measured for 211 PD patients and 135 healthy controls. Leukocyte 8-OHdG and plasma MDA were elevated, whereas erythrocyte GPx and plasma Vit E were reduced in PD patients when compared to the controls. After adjusting for environmental factors, logistic regression analysis showed that PD severity was independently correlated with 8-OHdG and MDA level, and inversely correlated with GPx activity and Vit E level. Leucocyte 8-OHdG level was continuously increased with advanced PD Hoehn-Yahr stages, while plasma MDA level peaked at early disease stages, among PD patients. These results suggest increased oxidative damage and decreased anti-oxidant capacity in peripheral blood, and a significant correlation between leucocyte 8-OHdG level and disease severity in PD.
authors.affiliation: Department of Neurology, Chang Gung Memorial Hospital, Chang-Gung University College of Medicine, Taipei, Taiwan. chengm@mail.cgu.edu.tw
authors.forename: Chiung-Mei
authors.initials: CM
authors.lastname: Chen
authors.forename: Jun-Liang
authors.initials: JL
authors.lastname: Liu
authors.forename: Yih-Ru
authors.initials: YR
authors.lastname: Wu
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Chen
authors.forename: Huey-Shinn
authors.initials: HS
authors.lastname: Cheng
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Cheng
authors.forename: Daniel Tsun-Yee
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000704:Analysis of Variance; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D016015:Logistic Models; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress; D010300:Parkinson Disease / Q000097:blood* / Q000503:physiopathology*; D012307:Risk Factors; D014810:Vitamin E / Q000097:blood
chemical_list: D014810:Vitamin E; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D003849:Deoxyguanosine