title: The nuclear orphan receptor COUP-TFII plays an essential role in adipogenesis, glucose homeostasis, and energy metabolism.
abstract: Adipose tissue development and function play a central role in the pathogenesis and pathophysiology of metabolic syndromes. Here, we show that chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) plays a pivotal role in adipogenesis and energy homeostasis. COUP-TFII is expressed in the early stages of white adipocyte development. COUP-TFII heterozygous mice (COUP-TFII(+/-)) have much less white adipose tissue (WAT) than wild-type mice (COUP-TFII(+/+)). COUP-TFII(+/-) mice display a decreased expression of key regulators for WAT development. Knockdown COUP-TFII in 3T3-L1 cells resulted in an increased expression of Wnt10b, while chromatin immunoprecipitation analysis revealed that Wnt10b is a direct target of COUP-TFII. Moreover, COUP-TFII(+/-) mice have increased mitochondrial biogenesis in WAT, and COUP-TFII(+/-) mice have improved glucose homeostasis and increased energy expenditure. Thus, COUP-TFII regulates adipogenesis by regulating the key molecules in adipocyte development and can serve as a target for regulating energy metabolism.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors.forename: Luoping
authors.initials: L
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: George S
authors.initials: GS
authors.lastname: Jeha
authors.forename: Pradip K
authors.initials: PK
authors.lastname: Saha
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Claire M
authors.initials: CM
authors.lastname: Haueter
authors.forename: Lawrence
authors.initials: L
authors.lastname: Chan
authors.forename: Sophia Y
authors.initials: SY
authors.lastname: Tsai
authors.forename: Ming-Jer
authors.initials: MJ
authors.lastname: Tsai
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D051837:COUP Transcription Factor II / Q000378:metabolism*; D002454:Cell Differentiation; D002645:Chickens; D004734:Energy Metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism*; D006579:Heterozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000209:etiology / Q000517:prevention & control; D013997:Time Factors; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D051837:COUP Transcription Factor II; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse; D005947:Glucose