title: Pax5 immunostaining in paraffin-embedded sections of canine non-Hodgkin lymphoma: a novel canine pan pre-B- and B-cell marker.
abstract: The Pax5 gene encodes the B-cell specific activator protein (BSAP), a member of the highly conserved paired box (PAX)-domain family of transcription factors and a key regulator in the development and differentiation of B-cells. Pax5 serves as a valuable B-cell marker in the classification of human lymphoma patients as it is restricted to lymphomas of B-cell lineage. In dogs, detection of Pax5 protein in lymphoma tissue has not been reported. Therefore, we have investigated the expression and detection of BSAP using a monoclonal anti-Pax5 antibody (anti-BSAP, clone 24) in canine lymphoma tissue samples to evaluate its diagnostic relevance as a B-cell marker. A series of 25 lymph nodes from 23 canine non-Hodgkin lymphoma patients, a reactive canine lymph node, and a normal non-reactive canine lymph node, were evaluated. All B-cell non-Hodgkin lymphomas (15) were found to express Pax5 protein. In addition, there was a strong correlation between Pax5 and CD79a expression. Three CD3 positive and five CD3 and CD79a positive lymphomas were immunophenotypically negative for anti-Pax5, indicating a T-cell lineage. In conclusion, anti-Pax5 antibody may offer an excellent B-cell marker in canine lymphomas.
authors.affiliation: Department for Companion Animals and Horses, Clinic for Internal Medicine and Infectious Diseases, University of Veterinary Medicine Vienna, Vienna, Austria. michael.willmann@vu-wien.ac.at
authors.forename: M
authors.initials: M
authors.lastname: Willmann
authors.forename: L
authors.initials: L
authors.lastname: Müllauer
authors.forename: A
authors.initials: A
authors.lastname: Guija de Arespacochaga
authors.forename: M
authors.initials: M
authors.lastname: Reifinger
authors.forename: I
authors.initials: I
authors.lastname: Mosberger
authors.forename: J G
authors.initials: JG
authors.lastname: Thalhammer
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D016130:Immunophenotyping / Q000662:veterinary; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D051757:PAX5 Transcription Factor / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D016612:Paraffin Embedding / Q000662:veterinary
chemical_list: D051757:PAX5 Transcription Factor