Evaluation of whole antioxidant defenses of human mononuclear cells by a new in vitro biological test: lack of correlation between erythrocyte and mononuclear cell resistance to oxidative stress.
OBJECTIVE
This work aims to evaluate the resistance of mononuclear cells to oxidative stress using a "KRL" test, formerly utilized to evaluate the resistance of erythrocyte to free radicals.


METHODS
The "KRL" test evaluates the resistance to lysis of cells treated by free radicals generated under standardized conditions.


RESULTS
We defined new analytical parameters (level of radical production, time course, number of cells) to obtain an accurate assay determining the resistance to oxidative stress of mononuclear cells, in comparison to that of erythrocytes. This test allows the evaluation of change in the redox state of mononuclear cells (improved by an antioxidant mix or deteriorated by antimycin A-induced mitochondrial radical overproduction). Interestingly, our data show that the sensitivity of mononuclear cells to oxidative stress is not correlated with the susceptibility of erythrocytes to oxidative stress.


CONCLUSIONS
The quantification of the susceptibility of mononuclear cells to oxidative stress gives additional information (in addition to erythrocyte resistance) and could be helpful for patients with chronic inflammation.
Caspar-Bauguil
Sylvie
S
Laboratoire de Biochimie, C.H.U. Rangueil, 1 av J.Poulhes, Toulouse, France. casparbauguil.s@chu-toulouse.fr
Maestre
Nicolas
N
Segafredo
Céline
C
Galinier
Anne
A
Garcia
Jésus
J
Prost
Michel
M
Périquet
Brigitte
B
Pénicaud
Luc
L
Salvayre
Robert
R
Casteilla
Louis
L
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D018929:Cell Culture Techniques; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013223:Statistics as Topic
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D011933:Reagent Kits, Diagnostic; D000968:Antimycin A; D014450:Electron Transport Complex III