Development of an enzyme-linked immunosorbent assay for residue analysis of the insecticide emamectin benzoate in agricultural products.
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3-3.0 ng/mL, and the 50% inhibition concentration (IC(50)) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 microg/g). Emamectin residues contain the following metabolites: the 4''-epi-amino analogue, the 4''-epi-(N-formyl)amino analogue, the 4''-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC(50) value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15-300 ng/g, and the recoveries were 91-117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products.
Kondo
Mika
M
Research and Development Department, Medical Electronic Systems Division, Horiba, Miyanohigashi, Kisshoin, Minami-ku, Kyoto, Japan.
Yamashita
Hiroshi
H
Uchigashima
Mikiko
M
Kono
Takeshi
T
Takemoto
Toshihide
T
Fujita
Masahiro
M
Saka
Machiko
M
Iwasa
Seiji
S
Ito
Shigekazu
S
Miyake
Shiro
S
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005506:Food Contamination; D007306:Insecticides / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D010573:Pesticide Residues / Q000032:analysis; D014675:Vegetables / Q000737:chemistry
D007306:Insecticides; D010573:Pesticide Residues; D007559:Ivermectin; C108024:emamectin benzoate