"Singapore Green": a new fluorescent dye for microarray and bioimaging applications.
We have synthesized Singapore Green (SG), a structural hybrid of Tokyo Green and Rhodamine 110. This new dye is a green light-emitting substitute for 7-aminocoumarin, a blue fluorescent dye widely used in enzymatic assays. SG-conjugated peptide substrates were successfully synthesized and used in microarray-based protease substrate profiling and live-cell imaging experiments.
Li
Junqi
J
Department of Chemistry, NUS MedChem Program of the Office of Life Sciences, National University of Singapore, Singapore 117543.
Yao
Shao Q
SQ
D020169:Caspases / Q000032:analysis / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D040081:Protein Array Analysis; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D012235:Rhodamines; D020169:Caspases