[Effects of resveratrol on the release of Glu and Gln in cultured rat astrocytes with stretch injury].
OBJECTIVE
To study the effects of resveratrol on amino acid release in cultured astrocytes with stretch injury and explore its possible mechanism for central nervous system protection.


METHODS
Cultured rat astrocytes were treated with different concentrations of resveratrol for 12 h and the subsequent changes in Glu and Gln release and lactate dehydrogenase(LDH) leakage were examined after stretch injury.


RESULTS
High-performance liquid chromatography showed that stretch injury increased Glu release from the astrocytes (P<0.05), and at the concentration of 1 micromol/L, resveratrol further increased Glu release (P<0.05), whereas at 100 micromol/L, resveratrol obviously inhibit Glu release (P<0.05). LDH leakage increased significantly after stretch injury of the astrocytes (P<0.05), and resveratrol acted to further increase LDH leakage at 1 micromol/L but inhibited LDH leakage at 100 micromol/L (P<0.05).


CONCLUSIONS
Resveratrol can inhibit the release of Glu and LDH leakage from rat astrocytes to protect the cells from stretch injury.
Zhou
Hao
H
Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. zhou_hao120@163.com
Chen
Qing
Q
Guo
Jiang
J
Wang
Qian
Q
Kong
Dan-li
DL
Yu
Shou-yi
SY
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005973:Glutamine / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013314:Stress, Mechanical
D018696:Neuroprotective Agents; D013267:Stilbenes; D005973:Glutamine; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D000077185:Resveratrol