Survival and differentiation of neuroepithelial stem cells following transplantation into the lateral ventricle of rats.
Neuroepithelial stem cells (NEPs) demonstrate a high potential for self-renewal and differentiation during embryonic development. To explore the survival and differentiation of NEPs in vivo, we isolated NEPs from green fluorescence protein (GFP) transgenic embryos and transplanted into the lateral ventricle of rats. In vitro culture, NEPs proliferated into neurospheres and differentiated into both neurons and glia. When transplanted into the lateral ventricle of rats, these GFP positive NEPs (GFP+ NEPs) survived and attached to the wall of ventricle. Moreover, grafted cells differentiated into neuron-specific enolase (NSE) positive neurons and glial fibrillary acidic protein (GFAP) positive astrocytes and migrated into the host brain. Thus, our results indicate that NEPs can survive and differentiate into neurons and astrocytes in the lateral ventricle following transplantation.
Sun
Jin-Hao
JH
Institute of Anatomy and Histology & Embryology, School of Medicine, Shandong University, Jinan 250012, People's Repulic of China. sunjinhao@sdu.edu.cn
Zheng
Pei-Hui
PH
Wang
Ling-Ling
LL
Li
Jing-Jing
JJ
Wang
Jia-Zeng
JZ
Wu
Qi
Q
Song
Tao
T
Liu
Wen-Jing
WJ
Gao
Ying-Mao
YM
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000166:cytology*; D046569:Neuroepithelial Cells / Q000166:cytology* / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins; D010751:Phosphopyruvate Hydratase