title: [Molecular techniques in mycology].
abstract: An increasing number of molecular techniques for the diagnosis of fungal infections have been developed in the last few years, due to the growing prevalence of mycoses and the length of time required for diagnosis when classical microbiological methods are used. These methods are designed to resolve the following aspects of mycological diagnosis: a) Identification of fungi to species level by means of sequencing relevant taxonomic targets; b) early clinical diagnosis of invasive fungal infections; c) detection of molecular mechanisms of resistance to antifungal agents; and d) molecular typing of fungi. Currently, these methods are restricted to highly developed laboratories. However, some of these techniques will probably be available in daily clinical practice in the near future.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, España. jlrtudela@isciii.es
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Rodríguez-Tudela
authors.forename: Isabel
authors.initials: I
authors.lastname: Cuesta
authors.forename: Alicia
authors.initials: A
authors.lastname: Gómez-López
authors.forename: Ana
authors.initials: A
authors.lastname: Alastruey-Izquierdo
authors.forename: Leticia
authors.initials: L
authors.lastname: Bernal-Martínez
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D000935:Antifungal Agents / Q000627:therapeutic use; D000946:Antigens, Fungal / Q000097:blood; D004271:DNA, Fungal / Q000032:analysis; D021901:DNA, Intergenic / Q000032:analysis; D025141:Drug Resistance, Fungal; D042241:Early Diagnosis; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007753:Laboratories / Q000600:supply & distribution; D025202:Molecular Diagnostic Techniques / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D000946:Antigens, Fungal; D004271:DNA, Fungal; D021901:DNA, Intergenic