High-potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa azurins.
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu(II/I) reduction potentials relative to the Cu(II) aquo ion. The high potential and low axial hyperfine splitting (Cu(II) electron paramagnetic resonance A( parallel)) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.
Lancaster
Kyle M
KM
Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA.
Yokoyama
Keiko
K
Richards
John H
JH
Winkler
Jay R
JR
Gray
Harry B
HB
D001400:Azurin / Q000235:genetics* / Q000378:metabolism; D003300:Copper; D004578:Electron Spin Resonance Spectroscopy; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D015202:Protein Engineering; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
D001400:Azurin; D003300:Copper