title: Immobilization of proteins on metal ion chelated polymer surfaces.
abstract: Over the last decade, the chelator-based strategy for protein immobilization has received considerable attention. Here, we describe a stepwise approach for the modification of polyurethane (PU) surfaces which involves the introduction of a poly(ethylene glycol) (PEG) layer to shield the PU substrate surface against nonspecific protein adsorption and a chelator head (quinolin-8-ol, HQ), to provide relatively high-target protein binding capacity. The surface properties, the immobilization of proteins on the surface, and the bioactivity of the immobilized proteins were investigated by various techniques. It was demonstrated that this approach provides a powerful means for surface immobilization of proteins with high density, with a homogeneous distribution and retaining the bioactivity of the immobilized proteins.
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Rd, Wuhan 430070, China.
authors.forename: Zhongkui
authors.initials: Z
authors.lastname: Wu
authors.forename: Libin
authors.initials: L
authors.lastname: Ding
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Song
mesh_terms: D000327:Adsorption; D002614:Chelating Agents / Q000378:metabolism*; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D055767:Immobilized Proteins / Q000378:metabolism*; D007477:Ions; D009532:Nickel / Q000378:metabolism*; D015125:Oxyquinoline / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D011140:Polyurethanes / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D013499:Surface Properties
chemical_list: D002614:Chelating Agents; D055767:Immobilized Proteins; D007477:Ions; D011108:Polymers; D011140:Polyurethanes; D011092:Polyethylene Glycols; D015125:Oxyquinoline; D009532:Nickel; D005340:Fibrinogen