Human immunodeficiency virus type 1-anchored CD40 ligand induces secretion of the chemokine interleukin-8 by human primary macrophages.
CD40 ligand (CD40L) is mainly expressed in activated CD4(+)T cells and interacts with CD40 on antigen-presenting cells to regulate both humoral and cellular immune responses. We previously reported that CD40L is acquired by emerging HIV-1 particles. Here we demonstrate that both wild-type and a non-functional mutated form of CD40L are incorporated within HIV-1. Importantly, we show that wild-type CD40L remains functional since CD40L-bearing virions mediate NF-kappaB activation in a CD40-expressing reporter cell line and induce secretion of the chemokine IL-8 by monocyte-derived macrophages. These results suggest a possible means exploited by HIV-1 to attract susceptible target cells to the site of infection, a process that might promote viral dissemination.
Maurais
Emilie
E
Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval, Canada.
Cantin
Réjean
R
Tremblay
Michel J
MJ
D023201:CD40 Ligand / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D015658:HIV Infections / Q000276:immunology* / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D009154:Mutation
D016209:Interleukin-8; D023201:CD40 Ligand