Recent advances in immobilized enzymatic reactors and their applications in proteome analysis.
Immobilized enzymatic reactors recently have drawn much attention because of the striking advantages, such as high substrate turnover rate and ease in coupling with the separation and detection systems. Carrier materials, which have great effects on the development of the immobilized enzymatic reactors, have always being the focus of study. In this paper, the contributions, mainly in the last 5 years, on the enzymatic reactors and their applications in proteome study are reviewed, with some newly developed inorganic and organic carriers for enzyme immobilization described in details. Moreover, the hyphenation of immobilized enzymatic reactors with the separation and identification systems is also summarized. By reviewing these achievements, it could be seen that enzymatic reactors have very bright future, especially in proteome analysis.
Ma
Junfeng
J
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Zhang
Lihua
L
Liang
Zhen
Z
Zhang
Weibing
W
Zhang
Yukui
Y
D004800:Enzymes, Immobilized / Q000032:analysis* / Q000378:metabolism*; D006801:Humans; D007287:Inorganic Chemicals / Q000737:chemistry; D055601:Organic Chemistry Phenomena; D011108:Polymers / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D004800:Enzymes, Immobilized; D007287:Inorganic Chemicals; D011108:Polymers; D020543:Proteome