title: Carboxylesterase activity, cDNA sequence, and gene expression in malathion susceptible and resistant strains of the cotton aphid, Aphis gossypii.
abstract: Levels of insecticide resistance, carboxylesterase activity, carboxylesterase expression, and the cDNA sequence of carboxylesterase gene were investigated in malathion resistant and susceptible strains of cotton aphids, Aphis gossypii (Glover). The resistant strain (MRR) exhibited 80.6-fold resistance to malathion compared to the susceptible strain (MSS) in cotton aphids. Five substrates, alpha-naphthyl acetate (alpha-NA), beta-naphthyl acetate (beta-NA), alpha-naphthyl propionate (alpha-NPr), alpha-naphthyl butyrate (alpha-NB), alpha-naphthyl caprylate (alpha-NC) and S-methyl thiobutyrate (S-MTB) were used to determine carboxylesterase activity in MRR and MSS strains of cotton aphids. Carboxylesterase activity was significantly higher in MRR strain than in MSS strain, 3.7-fold for alpha-NA, 3.0-fold for beta-NA, 2.0-fold for alpha-NPr, 2.9-fold for alpha-NB and 1.6-fold for alpha-NC, While for S-MTB, there was nearly no difference between the two strains. Two site mutations (K14Q and N354D) with high frequency were also found by sequence analysis in the MRR strain, compared with the MSS strain. The levels of gene expression for carboxylesterase of both MRR and MSS strains were determined by real-time quantitative PCRs. Compared with the MSS strain, the relative transcription levels and gene copy numbers of the carboxylesterase were 1.99- and 4.42-fold in the MRR strain, respectively. These results indicated that the increased expression of the carboxylesterase resulted from the increased transcription levels of carboxylesterase mRNA and gene copy numbers and combined with the site mutants might play role in cotton aphid resistance to malathion.
authors.affiliation: Department of Entomology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, China.
authors.forename: Yiou
authors.initials: Y
authors.lastname: Pan
authors.forename: Huilin
authors.initials: H
authors.lastname: Guo
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D001042:Aphids / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D043182:Carboxylesterase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics*; D008294:Malathion / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D009281:Naphthalenes / Q000494:pharmacology; D009284:Naphthols / Q000494:pharmacology; D063086:Organothiophosphates / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013835:Thiamine Pyrophosphate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D009281:Naphthalenes; D009284:Naphthols; D063086:Organothiophosphates; D011422:Propionates; D012333:RNA, Messenger; C005366:naphthyl acetate; C060294:1-naphthalenepropionic acid; C006863:butyl phosphorotrithioate; C027876:alpha-naphthyl acetate; C029204:alpha-naphthyl butyrate; D043182:Carboxylesterase; D013835:Thiamine Pyrophosphate; D008294:Malathion