Fast action of neuroactive steroids on plasma membrane calcium pump in PC12 cells.
Calcium ions are essential to proper neurotransmission. Impairment in cytosolic Ca(2+) concentration and Ca(2+) signaling disturbs neuronal activity, leading to pathological consequences. In cells, a high-affinity plasma membrane calcium pump (PMCA) keeps free Ca(2+) in the nanomolar range. Among four genes encoding the enzyme, PMCA2 and 3 are primary in excitable cells. To elucidate the relationship between PMCAs' composition and susceptibility for neurosteroid regulation, we obtained PC12 cells with suppressed neuron-specific isoforms and analyzed the effect of selected steroids on Ca(2+) uptake. Our results indicate that hormones affected Ca(2+) transport activity and that this effect depended on both PMCA isoform composition and steroid structure.
Zylinska
Ludmila
L
Department of Molecular Neurochemistry, Medical University, Lodz, Poland. luska@csk.umed.lodz.pl
Kowalska
Iwona
I
Kozaczuk
Anna
A
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism*; D051381:Rats; D013256:Steroids / Q000494:pharmacology*
D020033:Protein Isoforms; D013256:Steroids; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium