In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy.
Magnesium has shown potential application as a bio-absorbable biomaterial, such as for bone screws and plates. In order to improve the surface bioactivity, a calcium phosphate was coated on a magnesium alloy by a phosphating process (Ca-P coating). The surface characterization showed that a porous and netlike CaHPO(4).2H(2)O layer with small amounts of Mg(2+) and Zn(2+) was formed on the surface of the Mg alloy. Cells L929 showed significantly good adherence and significantly high growth rate and proliferation characteristics on the Ca-P coated magnesium alloy (p<0.05) in in-vitro cell experiments, demonstrating that the surface cytocompatibility of magnesium was significantly improved by the Ca-P coating. In vivo implantations of the Ca-P coated and the naked alloy rods were carried out to investigate the bone response at the early stage. Both routine pathological examination and immunohistochemical analysis demonstrated that the Ca-P coating provided magnesium with a significantly good surface bioactivity (p<0.05) and promoted early bone growth at the implant/bone interface. It was suggested that the Ca-P coating might be an effective method to improve the surface bioactivity of magnesium alloy.
Xu
Liping
L
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, China.
Pan
Feng
F
Yu
Guoning
G
Yang
Lei
L
Zhang
Erlin
E
Yang
Ke
K
D000497:Alloys / Q000494:pharmacology*; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005269:Femur / Q000187:drug effects / Q000473:pathology; D005453:Fluorescence; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008274:Magnesium / Q000494:pharmacology*; D008422:Materials Testing; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D019919:Prosthesis Implantation; D011817:Rabbits; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014961:X-Ray Diffraction
D000497:Alloys; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; C020243:calcium phosphate; D008274:Magnesium