A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24.
Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4pg to up to 20,000pgml(-1) in a volume of 50microl and can be combined with other measurements.
Biancotto
Angélique
A
Section on Intercellular Interactions, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
Brichacek
Beda
B
Chen
Silvia S
SS
Fitzgerald
Wendy
W
Lisco
Andrea
A
Vanpouille
Christophe
C
Margolis
Leonid
L
Grivel
Jean-Charles
JC
D005434:Flow Cytometry / Q000379:methods*; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D016655:HIV Core Protein p24 / Q000032:analysis*; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D008863:Microspheres; D012680:Sensitivity and Specificity
D016655:HIV Core Protein p24; C514799:p24 protein, Human Immunodeficiency Virus Type 1