Cytotoxic activity of alkaloids isolated from Stephania rotunda [corrected].
Three major alkaloids: cepharanthine (1), tetrahydropalmatine (2) and xylopinine (3) isolated from Stephania rotunda tuber were investigated for their cytotoxic activity in a panel of human cancer cells (HT29, LS174T, SW620 and HepG2) using MTT assay. In the present study, cepharanthine (1) exerted potent cytotoxicity against colon and hepatoma cancer cell lines with IC(50) values between 2.4 and 5.3 microM while tetrahydropalmatine (2) and xylopinine (3) displayed weak cytotoxicity. In addition, the mutagenic activity of cepharanthine (1) was investigated using a modified liquid incubation technique of the Salmonella/microsomal assay. This alkaloid (1) was found to be non-mutagenic for doses up to 8.2 microM.
Bun
Sok-Siya
SS
Laboratory of Pharmacognosy, UMR-MD3, Faculty of Pharmacy, Marseille, France. siya.bun@pharmacie.univ-mrs.fr
Laget
Michèle
M
Chea
Aun
A
Bun
Hot
H
Ollivier
Evelyne
E
Elias
Riad
R
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D012486:Salmonella typhimurium / Q000187:drug effects; D031607:Stephania / Q000737:chemistry*; D052117:Benzodioxoles
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; C453820:xylopinine; C014215:tetrahydropalmatine; C006947:cepharanthine; D052117:Benzodioxoles