[Protective effect of lytic cocktail (inhibition of stress) on lung injury in severe burn rat].
OBJECTIVE
To investigate the protective effect of inhibition of stress (lytic cocktail) on lung injury in severe burn rats at early stage.


METHODS
Sprague-Dawley rats inflicted with 30% TBSA full-thickness burn were randomly divided into A group (n = 36, fluid resuscitation with administration of lytic cocktail), B group (n = 36, fluid resuscitation only). Lung function was evaluated by partial pressure of oxygen (PaO2) in arterial blood and histopathologic changes on 3, 5, 7, 10 post burn day (PBD). The levels of malonyldialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-alpha(TNF-alpha) and interferon-gamma (IFN-gamma) in lung tissue were measured at the same time points.


RESULTS
The PaO2 level in A group on 3 PBD (12.58 +/- 0.41 kPa) was significantly higher than that in B group (8.86 +/- 0.23 kPa, P < 0.01). Compared with those in B group, the levels of MDA and MPO were significantly decreased in A group at each time point (P < 0.05 or 0.01), the levels of TNF-alpha on 3, 5, 7 PBD (P < 0.05 or 0.01) and IFN-gamma on 5, 7, 14 PBD (P < 0.01) were also decreased in A group. Swollen lung mesenchyme was alleviated, infiltration of inflammatory cell was lessened in A group.


CONCLUSIONS
Lytic cocktail combined with immediate fluid resuscitation can inhibit stess response, downregulate the expression of inflammatory factor, ameliorate lung function in severe burn rat at early stage.
Qiao
Liang
L
Department of Burns, Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, PR China.
Yuan
Ke-jian
KJ
Yang
Hui-zhong
HZ
Dong
He-liang
HL
Wang
Wen-kui
WK
Xu
Wei-shi
WS
D000818:Animals; D002056:Burns / Q000150:complications / Q000628:therapy*; D005440:Fluid Therapy; D007371:Interferon-gamma / Q000378:metabolism; D055370:Lung Injury / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D008315:Malondialdehyde / Q000378:metabolism; D008614:Meperidine / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D007371:Interferon-gamma; D008614:Meperidine; D009195:Peroxidase