CD3zeta down-modulation may explain Vgamma9Vdelta2 T lymphocyte anergy in HIV-infected patients.
The aim of the present study was to explain the observed anergy of Vgamma9Vdelta2 T cells from human immunodeficiency virus (HIV)-positive patients. CD3zeta expression and interferon (IFN)-gamma production by Vgamma9Vdelta2 T cells from HIV-positive and HIV-negative subjects were analyzed. We demonstrated that Vgamma9Vdelta2 T cells from HIV-infected patients expressed a lower level of CD3zeta than did Vgamma9Vdelta2 T cells from healthy donors. A direct correlation was found between CD3zeta expression and IFN-gamma production capability by Vgamma9Vdelta2 T cells. However, activation of protein kinase C by phorbol myristate acetate is able to restore CD3zeta expression and IFN-gamma production. Our findings may contribute to clarification of the molecular mechanisms of Vgamma9Vdelta2 T cell anergy found in HIV-positive patients.
Sacchi
Alessandra
A
Laboratory of Cellular Immunology, National Institute for Infectious Diseases Lazzaro Spallanzani, Rome, Italy. sacchi@inmi.it
Tempestilli
Massimo
M
Turchi
Federica
F
Agrati
Chiara
C
Casetti
Rita
R
Cimini
Eleonora
E
Gioia
Cristiana
C
Martini
Federico
F
D000328:Adult; D000368:Aged; D017252:CD3 Complex / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D017635:Clonal Anergy; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D055815:Young Adult
D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D016207:Cytokines; D007371:Interferon-gamma