[Analysis of heterogeneity in exon 4 of Coxsackievirus and Adenovirus receptor gene in dilated cardiomyopathy].
OBJECTIVE
To investigate the genetic pathogenesis of dilated cardiomyopathy (DCM) by examining the heterogeneity of Coxsackievirus binding domain (exon 4) of Adenovirus receptor (CAR) in DCM patients and healthy adults, and to detect possible mutation site in exon 4.


METHODS
Using polymerase chain reaction (PCR), we amplified exon 4 of CAR DNA extracted from blood samples obtained from 50 DCM patients and 40 healthy adults. The PCR products were screened with single-strand conformation polymorphism (SSCP) and then sequenced alternatively based on the SSCP results.


RESULTS
The segment of CAR exon 4 was successfully amplified and there was no single strand conformational disparity in all samples examined by SSCP. Sequence analysis demonstrated that all amplified sequences of CAR exon 4 from samples of the two groups were identical and there was no genetic heterogeneity of CAR exon 4 between the two groups.


CONCLUSIONS
The genetic heterogeneity of CAR exon 4 might not be responsible for the pathogenesis of DCM.
Zhang
Shuo
S
Department of Cardiology, Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
Teng
Lei
L
Jia
Hai-bo
HB
Li
Li-li
LL
Gao
Xue-qin
XQ
Yu
Bo
B
D000328:Adult; D001483:Base Sequence; D002311:Cardiomyopathy, Dilated / Q000235:genetics* / Q000821:virology*; D003384:Coxsackievirus Infections / Q000235:genetics; D004770:Enterovirus / Q000235:genetics*; D005091:Exons; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D011991:Receptors, Virus / Q000235:genetics*; D017422:Sequence Analysis, DNA
D011991:Receptors, Virus; C085301:adenovirus receptor