title: [Dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro].
abstract: OBJECTIVE
  To observe whether dendritic cells (DCs) transfected with recombinant adenovirus Ad5F35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro.
  
  
  METHODS
  Dendritic cells have been generated in vitro, and cocultured with autologous T cell after the DCs were infected with Ad5F35-LMP2, then the proliferation of the induced T cells and their cytotoxic activity against CNE-2 tumor cells which express EBV-LMP2 protein on membrane were assessed by MTT method.
  
  
  RESULTS
  The dendritic cells could be transfected with Ad5F35-LMP2 and the CTL activated by Ad5F35-LMP2-DC could effectively suppress the proliferation of CNE-2 cells compared with control groups.
  
  
  CONCLUSIONS
  The dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 showed cytotoxicity effect by activating T lymphocytes.
authors.affiliation: First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Wu-Ning
authors.initials: WN
authors.lastname: Mo
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: An-Zhou
authors.initials: AZ
authors.lastname: Tang
authors.forename: Guang-Wu
authors.initials: GW
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000276:immunology* / Q000821:virology; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007111:Immunity, Cellular; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins