title: Genetic analysis of group A streptococcus isolates recovered during acute glomerulonephritis outbreaks in Guizhou Province of China.
abstract: In this study, 68 group A streptococcus (GAS) isolates associated with two outbreaks of acute glomerulonephritis (AGN) in China were analyzed by emm typing. A total of 11 different emm types were identified. Analysis of emm type distribution suggested that AGN outbreaks in two counties were caused by emm60.1- and emm63.0-type GAS. These two types were further characterized by pulsed-field gel electrophoresis, multilocus sequence typing, sof sequence typing, and PCR-based identification of streptococcal pyrogenic exotoxin A, B, and C (speA, speB, and speC) genes. In antimicrobial susceptibility tests, all outbreak strains were resistant to erythromycin and tetracycline, and the rates of resistance of nonoutbreak strains to the two antibiotics were 63.6% and 90.9%. This study is also the first to report a nephritogenic M63 GAS strain.
authors.affiliation: Department of Diagnosis, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China.
authors.forename: Ming-huan
authors.initials: MH
authors.lastname: Zheng
authors.forename: Zhen-quan
authors.initials: ZQ
authors.lastname: Jiao
authors.forename: Li-jie
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Sang-jie
authors.initials: SJ
authors.lastname: Yu
authors.forename: Guang-peng
authors.initials: GP
authors.lastname: Tang
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Yan
authors.forename: Li-hua
authors.initials: LH
authors.lastname: He
authors.forename: Fan-liang
authors.initials: FL
authors.lastname: Meng
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.forename: Mao-jun
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Xiao
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Nie
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Zi-jun
authors.initials: ZJ
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002352:Carrier Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D005921:Glomerulonephritis / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C014689:streptococcal M protein