Protein profilings in mouse liver regeneration after partial hepatectomy using iTRAQ technology.
Liver is unique in its capability to regenerate after an injury. Liver regeneration after a 2/3 partial hepatectomy served as a classical model and is adopted frequently to study the mechanism of liver regeneration. In the present study, semiquantitative analysis of protein expression in mouse liver regeneration following partial hepatectomy was performed using an iTRAQ technique. Proteins from pre-PHx control livers and livers regenerating for 24, 48 and 72 h were extracted and inspected using 4-plex isotope labeling, followed by liquid chromatography fractionation, mass spectrometry and statistical differential analysis. A total of 827 proteins were identified in this study. There were 270 proteins for which quantitative information was available at all the time points in both biologically duplicate experiments. Among the 270 proteins, Car3, Mif, Adh1, Lactb2, Fabp5, Es31, Acaa1b and LOC100044783 were consistently down-regulated, and Mat1a, Dnpep, Pabpc1, Apoa4, Oat, Hpx, Hp and Mt1 were up-regulated by a factor of at least 1.5 from that of the controls at one time point or more. The regulation of each differential protein was also demonstrated by monitoring its time-dependent expression changes during the regenerating process. We believe this is the first report to profile the protein changes in liver regeneration utilizing the iTRAQ proteomic technique.
Hsieh
Hui-Chu
HC
Biomedical Engineering Research Laboratories, Industrial Technology Research Institute, Hsinchu 310, Taiwan.
Chen
Yi-Ting
YT
Li
Jen-Ming
JM
Chou
Ting-Yu
TY
Chang
Ming-Fong
MF
Huang
See-Chang
SC
Tseng
Tzu-Ling
TL
Liu
Chung-Cheng
CC
Chen
Sung-Fang
SF
D000818:Animals; D005260:Female; D006498:Hepatectomy; D007553:Isotope Labeling / Q000379:methods*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008110:Liver Extracts / Q000737:chemistry; D008115:Liver Regeneration / Q000502:physiology*; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D015203:Reproducibility of Results
D008110:Liver Extracts; D011506:Proteins; D020543:Proteome