title: Characterization of a Vitellogenin Gene Fragment in Boophilus microplus Ticks.
abstract: The objective of this study was to isolate, clone, and characterize a fragment of the vitellogenin (Vg) gene from a B. microplus tick strain from Mexico. Using cDNA and specific primers, an 1800-bp fragment was amplified, cloned, and transformed in into E. coli, and then sequenced. Comparative analysis with a previously reported sequence showed 99% identity at both the nucleotide and amino acid level. The predicted amino acid sequence of the Mexican Vg has 6 positive mutations. There is an insertion of an aspartic acid on position 26 and a deletion on position 552 with respect to the reported sequence. There were 11 predicted glycosylation sites conserved in both strains. It is concluded that there is a high sequence homology of Vg in both strains.
authors.affiliation: FMVZ, Universidad Nacional Autonoma de México, D.F. México, México.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Granjeno-Colin
authors.forename: Ruben
authors.initials: R
authors.lastname: Hernandez-Ortiz
authors.forename: Juan
authors.initials: J
authors.lastname: Mosqueda
authors.forename: Sandino
authors.initials: S
authors.lastname: Estrada-Mondaca
authors.forename: Julio V
authors.initials: JV
authors.lastname: Figueroa
authors.forename: Zeferino
authors.initials: Z
authors.lastname: Garcia-Vazquez
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006031:Glycosylation; D009154:Mutation; D016133:Polymerase Chain Reaction; D013987:Ticks / Q000235:genetics*; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014819:Vitellogenins