title: Jaagsiekte sheep retrovirus is present at high concentration in lung fluid produced by ovine pulmonary adenocarcinoma-affected sheep and can survive for several weeks at ambient temperatures.
abstract: Jaagsiekte sheep retrovirus (JSRV) causes a fatal lung cancer of sheep known as ovine pulmonary adenocarcinoma (OPA). OPA is a significant disease in many sheep-rearing countries and there is no effective method of control. A unique feature of OPA is the overproduction of fluid in the lung of affected animals. This lung fluid contains JSRV and provides a means of transmission through the inhalation of virus. In this study we demonstrated that lung fluid from different OPA cases contained between 10(7) and 10(10) copies of JSRV RNA per ml. Examination of JSRV RNA survival under conditions that mimic natural conditions suggested that intact JSRV virions may persist for several weeks in the environment. These are the first quantitative data on JSRV in lung fluid and provide valuable information for implementing appropriate biosecurity measures to control the spread of JSRV in the field.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Edinburgh EH26 0PZ, UK. chris.cousens@moredun.ac.uk
authors.forename: Christina
authors.initials: C
authors.lastname: Cousens
authors.forename: Leenadevi
authors.initials: L
authors.lastname: Thonur
authors.forename: Stuart
authors.initials: S
authors.lastname: Imlach
authors.forename: Joanne
authors.initials: J
authors.lastname: Crawford
authors.forename: Jill
authors.initials: J
authors.lastname: Sales
authors.forename: David J
authors.initials: DJ
authors.lastname: Griffiths
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000821:virology*; D022141:Jaagsiekte sheep retrovirus / Q000302:isolation & purification* / Q000502:physiology*; D008168:Lung / Q000821:virology*; D011648:Pulmonary Adenomatosis, Ovine / Q000821:virology*; D012756:Sheep; D013696:Temperature; D013997:Time Factors