Dietary calcium and 1,25-dihydroxyvitamin D3 regulate transcription of calcium transporter genes in calbindin-D9k knockout mice.
The effect(s) of oral calcium and vitamin D(3) were examined on the expression of duodenal and renal active calcium transport genes, i.e., calbindin-D9k (CaBP-9k) and calbindin-D28k (CaBP-28k), transient receptor potential cation channels (TRPV5 and TRPV6), Na(+)/Ca(2+) exchanger 1 (NCX1) and plasma membrane calcium ATPase 1b (PMCA1b), in CaBP-9k KO mice. Wild-type (WT) and KO mice were provided with calcium and vitamin D(3)-deficient diets for 10 weeks. The deficient diet significantly decreased body weights compared with the normal diet groups. The serum calcium concentration of the WT mice was decreased by the deficient diet but was unchanged in the KO mice. The deficient diet significantly increased duodenal transcription of CaBP-9k and TRPV6 in the WT mice, but no alteration was observed in the KO mice. In the kidney, the deficient diet significantly increased renal transcripts of CaBP-9k, TRPV6, PMCA1b, CaBP-28k and TRPV5 in the WT mice but did not alter calcium-relating genes in the KO mice. Two potential mediators of calcium-processing genes, vitamin D receptor (VDR) and parathyroid hormone receptor (PTHR), have been suggested to be useful for elucidating these differential regulations in the calcium-related genes of the KO mice. Expression of VDR was not significantly affected by diet or the KO mutation. Renal PTHR mRNA levels were reduced by the diet, and reduced expression was also seen in the KO mice given the normal diet. Taken together, these results suggest that the active calcium transporting genes in KO mice may have resistance to the deficiency diet of calcium and vitamin D(3).
Ko
Sang-Hwan
SH
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Chungbuk, Korea.
Lee
Geun-Shik
GS
Vo
Thuy T B
TT
Jung
Eui-Man
EM
Choi
Kyung-Chul
KC
Cheung
Ki-Wha
KW
Kim
Jae Wha
JW
Park
Jong-Gil
JG
Oh
Goo Taeg
GT
Jeung
Eui-Bae
EB
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D064092:Calbindin 1; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology*; D002136:Calcium, Dietary / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D044168:Receptor, Parathyroid Hormone, Type 1 / Q000096:biosynthesis / Q000235:genetics; D018167:Receptors, Calcitriol / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000096:biosynthesis / Q000235:genetics; D050916:TRPV Cation Channels / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
C576499:Calb1 protein, mouse; D064092:Calbindin 1; D064026:Calbindins; D002136:Calcium, Dietary; C499849:NCX1 protein, mouse; D012333:RNA, Messenger; D044168:Receptor, Parathyroid Hormone, Type 1; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; C576507:S100g protein, mouse; D019831:Sodium-Calcium Exchanger; D050916:TRPV Cation Channels; D053499:Plasma Membrane Calcium-Transporting ATPases; C506954:Atp2b1 protein, mouse; D002117:Calcitriol