title: An investigation of hormesis of trichloroethylene in L-02 liver cells by differential proteomic analysis.
abstract: Hormesis is the dose-response pattern of the biological responses to toxic chemicals, characterized by low-dose stimulation and high-dose inhibition. Although it is known that some cell types exhibit an adaptive response to low levels of cytotoxic agents, its molecular mechanism is still unclear and it has yet to be established whether this is a universal phenomenon that occurs in all cell types in response to exposure to every chemical. Trichloroethylene (TCE) is an organic solvent widely used and is released into the atmosphere from industrial degreasing operations. Acute (short-term) and chronic (long-term) inhalation exposure to trichloroethylene can affect the human health. In order to elucidate a cell-survival adaptive response of L-02 liver cells exposed to low dose of TCE, CCK-8 assay was used to assess cytotoxicity, and examined the possible mechanisms of hormesis by proteomics technology. We found that exposure of L-02 liver cells to low level of TCE resulted in adaptation to further exposure to higher level, about 1,000 protein-spots were obtained by two-dimensional electrophoresis (2-DE) and five protein spots were identified by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry sequencing of tryptic peptides. Our results suggest that a relationship may exist between identified proteins and TCE-induced hormesis, which are very useful for further study of the mechanism and risk assessment of TCE.
authors.affiliation: Toxicology Laboratory, Shenzhen Center for Disease Control and Prevention, 518020 Shenzhen, Guangdong, People's Republic of China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Huang
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Ren-Rong
authors.initials: RR
authors.lastname: Xi
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Xing
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Yuan
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Yang
authors.forename: Gong-Hua
authors.initials: GH
authors.lastname: Tao
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Gong
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Zhuang
mesh_terms: D000704:Analysis of Variance; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014241:Trichloroethylene / Q000494:pharmacology*
chemical_list: D011506:Proteins; D020543:Proteome; D014241:Trichloroethylene; D005982:Glutathione Transferase