Bone morphogenetic protein 7 (BMP-7) increases the expression of follicle-stimulating hormone (FSH) receptor in human granulosa cells.
OBJECTIVE
To examine the effect of bone morphogenetic protein 7 (BMP-7) on FSH receptor (FSHR) expression in human granulosa cells.


METHODS
Laboratory study using human samples.


METHODS
University hospital.


METHODS
Human granulosa cells were obtained from 60 women undergoing oocyte retrieval for IVF.


METHODS
Human granulosa cells (GCs) were cultured with recombinant BMP-7, followed by RNA extraction.


METHODS
mRNA levels of GCs were measured by real-time reverse-transcription polymerase chain reaction.


RESULTS
Bone morphogenetic protein 7 increased FSHR gene expression in human luteinized granulosa cells, whereas it decreased LH receptor gene expression. Bone morphogenetic protein 7 also increased FSH-induced cyclic adenosine monophosphate production in GCs, indicating up-regulation of the cellular response to FSH. Although BMP-7 increased gene expression of activin-betaA and -betaB in GCs, inhibition of activin function did not affect the BMP-7-induced FSHR gene expression.


CONCLUSIONS
These findings provide new insight into the biologic function of BMP-7 in the human ovary and demonstrate its unique mechanism of regulating FSHR action.
Shi
Jia
J
Department of Obstetrics and Gynecology, University of Tokyo, Tokyo 113-8655, Japan.
Yoshino
Osamu
O
Osuga
Yutaka
Y
Nishii
Osamu
O
Yano
Tetsu
T
Taketani
Yuji
Y
D055419:Bone Morphogenetic Protein 7 / Q000502:physiology*; D052004:Bone Morphogenetic Protein Receptors / Q000819:agonists / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D011962:Receptors, FSH / Q000096:biosynthesis* / Q000235:genetics; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics
D055419:Bone Morphogenetic Protein 7; D012333:RNA, Messenger; D011962:Receptors, FSH; D011974:Receptors, LH; D052004:Bone Morphogenetic Protein Receptors