title: Detection of TT virus (TTV) by three frequently-used PCR methods targeting different regions of viral genome in children with cryptogenic hepatitis, chronic B hepatitis and hbs carriers.
abstract: This study was designed so that three sensitive and widely-used polymerase chain reaction (PCR) methods for the detection of TT virus or Torque Teno virus (TTV) would be simultaneously applied to a large number of subjects to evaluate performances of the various PCR protocols with different genotype sensitivities. Sera were collected from 92 children admitted to Hacettepe University Ihsan Doğramaci Children's Hospital Pediatric Gastroenterology Unit (17 cryptogenic chronic hepatitis, 17 asymptomatic HBs carriers, 18 chronic HBV patients and 40 healthy children). TTV DNA was detected via nested N22, nested 3'-UTR and 5'-UTR PCRs for all samples. Differences in TTV D N A detection prevalences were n o t statistically significantbetween the study groups with all TTV DNA and liver enzyme levels. A significant agreement between PCR methods that target UTR was observed. TTV detection rate increased with age, suggesting a non-parenteral, environmental exposure to the virus for the study population.
authors.affiliation: Department of Clinical Microbiology, Hacettepe University Faculty of Medicine, Ankara, Turkey.
authors.forename: Koray
authors.initials: K
authors.lastname: Ergünay
authors.forename: Figen
authors.initials: F
authors.lastname: Gürakan
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Usta
authors.forename: Aysel
authors.initials: A
authors.lastname: Yüce
authors.forename: Hasan Ozen Erdem
authors.initials: HO
authors.lastname: Karabulut
authors.forename: Semsettin
authors.initials: S
authors.lastname: Ustaçelebi
mesh_terms: D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D016679:Genome, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D022783:Torque teno virus / Q000235:genetics*
chemical_list: D004279:DNA, Viral