title: Activation of muscarinic M-3 receptor may decrease glucose uptake and lipolysis in adipose tissue of rats.
abstract: Role of muscarinic receptor in the regulation of glucose uptake or lipolysis in adipose tissue remained unclear. In epididymal white adipose tissue (WAT) isolated from Wistar rats, we observed that acetylcholine (ACh) attenuated the insulin-stimulated glucose uptake and the release of glycerol from WAT in a concentration-dependent manner. Using the blockade of specific antagonists, both actions of ACh were characterized mainly due to an activation of M3 receptors. In the presence of various inhibitors for PLC-PKC pathway, ACh-decreased glucose uptake was also reversed. Taken together, these results suggest that muscarinic M3 receptor is involved in the regulation of glucose uptake and/or lipolysis in adipose tissue.
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung City, Taiwan, ROC.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Yang
authors.forename: Cheng-Kuei
authors.initials: CK
authors.lastname: Chang
authors.forename: Chiung-Wen
authors.initials: CW
authors.lastname: Tsao
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Hsu
authors.forename: Chao-Tien
authors.initials: CT
authors.lastname: Hsu
authors.forename: Juei-Tang
authors.initials: JT
authors.lastname: Cheng
mesh_terms: D000109:Acetylcholine / Q000378:metabolism* / Q000494:pharmacology; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007329:Insulin Antagonists / Q000378:metabolism / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D043587:Receptor, Muscarinic M3 / Q000187:drug effects / Q000378:metabolism*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D007328:Insulin; D007329:Insulin Antagonists; D043587:Receptor, Muscarinic M3; D010738:Type C Phospholipases; D005947:Glucose; D000109:Acetylcholine; D005990:Glycerol