Microarray-based approach identifies microRNAs and their target functional patterns in polycystic kidney disease.
BACKGROUND
MicroRNAs (miRNAs) play key roles in mammalian gene expression and several cellular processes, including differentiation, development, apoptosis and cancer pathomechanisms. Recently the biological importance of primary cilia has been recognized in a number of human genetic diseases. Numerous disorders are related to cilia dysfunction, including polycystic kidney disease (PKD). Although involvement of certain genes and transcriptional networks in PKD development has been shown, not much is known how they are regulated molecularly.


RESULTS
Given the emerging role of miRNAs in gene expression, we explored the possibilities of miRNA-based regulations in PKD. Here, we analyzed the simultaneous expression changes of miRNAs and mRNAs by microarrays. 935 genes, classified into 24 functional categories, were differentially regulated between PKD and control animals. In parallel, 30 miRNAs were differentially regulated in PKD rats: our results suggest that several miRNAs might be involved in regulating genetic switches in PKD. Furthermore, we describe some newly detected miRNAs, miR-31 and miR-217, in the kidney which have not been reported previously. We determine functionally related gene sets, or pathways to reveal the functional correlation between differentially expressed mRNAs and miRNAs.


CONCLUSIONS
We find that the functional patterns of predicted miRNA targets and differentially expressed mRNAs are similar. Our results suggest an important role of miRNAs in specific pathways underlying PKD.
Pandey
Priyanka
P
Medical Research Center, University Hospital Mannheim, Mannheim, Germany. priyankashahi2001@gmail.com
Brors
Benedikt
B
Srivastava
Prashant K
PK
Bott
Andrea
A
Boehn
Susanne N E
SN
Groene
Herrmann-Josef
HJ
Gretz
Norbert
N
D000818:Animals; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000235:genetics*; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D007690:Polycystic Kidney Diseases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D055647:Rats, Transgenic
D035683:MicroRNAs; D012333:RNA, Messenger