title: [Soft-tissue pyogenic infection in neonates caused by Staphylococcus aureus carrying Panton-Valentine leukocidin genes].
abstract: OBJECTIVE
  To investigate the pathogen causing soft-tissue pyogenic infection in neonate.
  
  
  METHODS
  The isolates of Staphylococcus aureus were obtained from liquor puris and blood by routine method. The Automated Microbiology Analyzer was used for identification and antimicrobial susceptibility test of the isolates. Panton-Valentine leukocidin (PVL) genes were determined by multiplex PCR in the isolates of Staphylococcus aureus. Multilocus sequence typing (MLST) was used to determine the sequence types (STs) of the isolates. The genotypes of SCCmec were also determined by another multiplex PCR in the isolates of methicillin-resistant Staphylococcus aureus (MRSA).
  
  
  RESULTS
  In 3 cases of neonate with soft-tissue pyogenic infection, 2 strains of Staphylococcus aureus isolated from liquor puris in 2 cases. 2 strains of Staphylococcus aureus were isolated from liquor puris and blood from another case. All 4 isolates were methicillin-resistant Staphylococcus aureus (MRSA) strains carrying PVL genes. Their SCCmec types were SCCmec IIIA. The STs of 4 isolates were ST88. The antimicrobial-resistance profile of the isolates were the same except erythromycin.
  
  
  CONCLUSIONS
  Soft-tissue pyogenic infection in the 3 neonates was caused by the same clone of MRSA carrying PVL genes.
authors.affiliation: Department of Clinical Laboratory, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Fang-you
authors.initials: FY
authors.lastname: Yu
authors.forename: Cun-li
authors.initials: CL
authors.lastname: Liu
authors.forename: Xue-qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Zeng-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Wang
authors.forename: Zhan-guo
authors.initials: ZG
authors.lastname: Chen
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D007956:Leukocidins / Q000235:genetics*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D058885:Multilocus Sequence Typing; D018461:Soft Tissue Infections / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin