title: Inhibitory effect of small interfering RNA specific for a novel candidate target in PB1 gene of influenza A virus.
abstract: Influenza, mainly caused by influenza virus, is becoming one of the major concerns in the world. Limitation in vaccines necessitates the urgent development of new therapeutic options against this virus. In the present study, we designed small interfering RNA (siRNA) targeting overlapping gene of PB1 and PB1-F2 gene of the influenza A virus and investigated its effect against influenza A virus infection. A reduction in virus-associated cell apoptosis was observed in A549 cells treated with this siRNA. Furthermore, its antiviral effect was confirmed by different methods. Also, a marked decrease of virus titer in chicken embryos treated with the siRNA was observed. The findings of this work highlight the potential of this shared region to be an additional therapeutic target for the treatment of influenza virus infection.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Xuanwu District, Beijing, PR China.
authors.forename: Congsheng
authors.initials: C
authors.lastname: Cheng
authors.forename: Lihong
authors.initials: L
authors.lastname: Yao
authors.forename: Aijun
authors.initials: A
authors.lastname: Chen
authors.forename: Runqing
authors.initials: R
authors.lastname: Jia
authors.forename: Lifang
authors.initials: L
authors.lastname: Huan
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Bo
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Shu
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002642:Chick Embryo; D004285:Dogs; D016679:Genome, Viral; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000502:physiology*; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication
chemical_list: C442816:PB1-F2 protein, Influenza A virus; D034741:RNA, Small Interfering; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1