Detection and chromatographic removal of lipopolysaccharide in preparations of multifunctional galectins.
The functional spectrum of human galectins is currently explored, with a wide range of activities being described. The role of galectin-3 as adhesin for bacteria is based on its strong binding to lipopolysaccharides (LPSs), which brings the possibility of such a contamination in galectin preparations to awareness. This assumption was verified in three independent functional assay systems using polymyxin B as inhibitor of LPS-dependent effects. Moreover, a commercial LPS quantification kit also revealed LPS in galectin preparations. Chromatography was effective in removing LPS, suggesting that such a technique needs to be applied to prevent assigning cellular responses to galectins rather than LPS.
Sarter
Kerstin
K
Department of Internal Medicine 3, Friedrich-Alexander University of Erlangen-Nuremberg, Erlangen, Germany.
André
Sabine
S
Kaltner
Herbert
H
Lensch
Martin
M
Schulze
Connie
C
Urbonaviciute
Vilma
V
Schett
Georg
G
Herrmann
Martin
M
Gabius
Hans-Joachim
HJ
D000818:Animals; D002460:Cell Line; D002845:Chromatography; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism; D037502:Galectin 3 / Q000096:biosynthesis / Q000494:pharmacology; D037161:Galectins / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D011112:Polymyxin B / Q000494:pharmacology; D011485:Protein Binding; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000737:chemistry* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D037502:Galectin 3; D037161:Galectins; D008070:Lipopolysaccharides; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011112:Polymyxin B