title: Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase.
abstract: The MCM gene from the archaeon Halobacterium, with and without its intein, was cloned into an Escherichia coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the intein, could be detected.
authors.affiliation: University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, 9600 Gudelsky Drive, Rockville, MD 20850, USA.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Sakakibara
authors.forename: Mimi
authors.initials: M
authors.lastname: Han
authors.forename: Claire R
authors.initials: CR
authors.lastname: Rollor
authors.forename: Rebecca C
authors.initials: RC
authors.lastname: Gilson
authors.forename: Courtney
authors.initials: C
authors.lastname: Busch
authors.forename: Gunyoung
authors.initials: G
authors.lastname: Heo
authors.forename: Zvi
authors.initials: Z
authors.lastname: Kelman