Protective effect of the extracts from Cnidium officinale against oxidative damage induced by hydrogen peroxide via antioxidant effect.
The dried rhizomes of Cnidium officinale are used as herbal drugs in the treatment of pain, inflammation, menstrual disturbance and antivitamin deficiency disease, and also act as a blood pressure depressant. In addition, there are several reports suggesting that they have pharmacological properties to tumor metastasis and angiogenesis, and that they act as an inhibitor of high glucose-induced proliferation of glomerular mesangial cells. However, little has been known about the functional role of the extracts from C. officinale on oxidative DNA damage and apoptosis caused by ROS. In this work, we have investigated the DPPH radical, hydroxyl radical and intracellular ROS scavenging capacity, and Fe(2+) chelating activity of the extracts from C. officinale. In addition, we evaluated whether the extracts are capable of reducing H(2)O(2)-induced DNA and cell damage in the human skin fibroblast cell. These extracts showed a dose-dependent free-radical scavenging capacity and a protective effect on DNA damage and the lipid peroxidation causing the cell damage by ROS. These antioxidant activities and inhibitory effects of the extracts on DNA and cell damage may further explain that C. officinale is useful as a herbal medicine for cancer chemoprevention.
Jeong
Jin Boo
JB
College of Natural Sciences, Andong National University, Andong, Republic of Korea.
Park
Jae Ho
JH
Lee
Hee Kyeong
HK
Ju
So Yeong
SY
Hong
Se Chul
SC
Lee
Jeong Rak
JR
Chung
Gyu Young
GY
Lim
Jae Hwan
JH
Jeong
Hyung Jin
HJ
D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D031048:Cnidium / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*
D000975:Antioxidants; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010936:Plant Extracts; D006861:Hydrogen Peroxide