A cyp19a1b-gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells.
Aromatase is an enzyme that catalyzes the synthesis of estrogen in gonads and brain. Teleost fish express aromatase (AroB) strongly in the brain facilitating its detailed examination. To understand the function of AroB in the brain, we generated transgenic zebrafish that expresses green fluorescent protein (GFP) driven by the brain aromatase cyp19a1b promoter. GFP was found in the radial glial cells of transgenic larvae and adult fish that overlap with AroB immunoreactivity in the correct temporal and spatial pattern. GFP was also coexpressed with radial cell marker BLBP, but was not in neurons. In addition, GFP expression in the radial glial cells was stimulated by estrogen, same as endogenous AroB expression. Thus, this transgenic line faithfully mimics the regulation of AroB expression in radial glial cells. It provides a powerful tool to further characterize progenitor radial cells in adult and developing fish and to evaluate estrogenic activities of xenoestrogens and phytoestrogens.
Tong
Sok-Keng
SK
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Mouriec
Karen
K
Kuo
Ming-Wei
MW
Pellegrini
Elisabeth
E
Gueguen
Marie-Madeleine
MM
Brion
François
F
Kah
Olivier
O
Chung
Bon-chu
BC
D000818:Animals; D030801:Animals, Genetically Modified; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D020847:Estrogen Receptor Modulators / Q000494:pharmacology; D005260:Female; D000077267:Fulvestrant; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D020847:Estrogen Receptor Modulators; D011993:Recombinant Fusion Proteins; D029961:Zebrafish Proteins; D049452:Green Fluorescent Proteins; D000077267:Fulvestrant; D004958:Estradiol; D001141:Aromatase; C440729:Cyp19a1a protein, zebrafish