title: 2-Aminobenzothiazole degradation by free and Ca-alginate immobilized cells of Rhodococcus rhodochrous.
abstract: 2-Aminobenzothiazole (ABT) degradation was investigated using free and immobilized systems during photodegradation under solar light in the presence of Fe(III)-nitrilotriacetic acid (FeNTA), biodegradation by Rhodococcus rhodochrous, and during combined conditions. Ca-alginate hydrogel was chosen as a model matrix and some complementary studies were required to characterize this new system. R. rhodochrous metabolism in this type of environment was monitored by NMR spectroscopy. Neither change in intracellular pH values nor in ATP concentrations was observed by in vivo(31)P NMR, showing that no metabolic modification occurred between free and immobilized cells. (1)H NMR demonstrated that alginate was not used as carbon source by R. rhodochrous. After establishing the pre-treatment protocol by SPE to eliminate solubilised alginate, ABT adsorption on beads and degradation were studied. The same pathways of transformation were observed in suspended and immobilized cell systems. Considering the ABT adsorption phenomenon on alginate beads (8%), the efficiency of the two systems was found to be comparable although the degradation rate was slightly lower with immobilized cells. The most important result was the finding that the positive effect of FeNTA on ABT degradation with immobilized cells was similar to that observed previously with free cells. All these results show that mechanisms observed with free cells can be extrapolated to entrapped cells, i.e. under conditions much closer to those usually encountered in the environment.
authors.affiliation: Laboratoire de Synthèse Et Etude de Systèmes à Intérêt Biologique, UMR 6504 CNRS, Université Blaise Pascal, 63177 Aubière Cedex, France.
authors.forename: Charlène
authors.initials: C
authors.lastname: Chorao
authors.forename: Franck
authors.initials: F
authors.lastname: Charmantray
authors.forename: Pascale
authors.initials: P
authors.lastname: Besse-Hoggan
authors.forename: Martine
authors.initials: M
authors.lastname: Sancelme
authors.forename: Angela
authors.initials: A
authors.lastname: Cincilei
authors.forename: Mounir
authors.initials: M
authors.lastname: Traïkia
authors.forename: Gilles
authors.initials: G
authors.lastname: Mailhot
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Delort
mesh_terms: D000464:Alginates / Q000737:chemistry*; D052160:Benzothiazoles / Q000378:metabolism*; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D002853:Chromatography, Liquid; D005290:Ferric Compounds / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D010782:Photolysis; D012240:Rhodococcus / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000464:Alginates; D052160:Benzothiazoles; D005290:Ferric Compounds; D006603:Hexuronic Acids; C523775:aminobenzothiazole compound; D020723:Glucuronic Acid; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate