Evaluation of the control of pathogen load by an anti-Salmonella bacterium in a herd of cattle with persistent Salmonella infection.
OBJECTIVE
To determine whether an anti-Salmonella bacterium is involved in control of pathogen load in persistently infected cattle herds.


METHODS
24 Holstein calves experimentally infected and 39 Holstein cows naturally infected with Salmonella spp.


METHODS
An Escherichia coli (designated as P8E5) that possessed anti-Salmonella activity was isolated from Salmonella-negative bovine feces obtained from a herd with endemic Salmonella infection. In vitro analysis involved enumerating Salmonella enterica serovar Typhimurium coincubated with E coli P8E5. In vivo analysis involved coadministration of Salmonella spp and E coli P8E5 or an E coli control strain to neonatal Holstein calves. Fecal samples were collected on multiple days after inoculation, and quantitative PCR assay was performed by use of Salmonella-specific primers.


RESULTS
E coli P8E5 reduced viability of Salmonella spp in vitro. Shedding of Salmonella organisms was diminished in calves administered E coli P8E5, whereas the control strain of E coli had no effect on shedding of Salmonella organisms.


CONCLUSIONS
In this study, an E coli strain was identified that possessed bacteriocin-like activity and was able to decrease viability of Salmonella organisms in vitro and in vivo. Therefore, it is possible that this organism could be representative of native microbiota that dampen Salmonella spp in persistently infected cattle herds.
Patton
Toni G
TG
Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2300 Dayton Rd, Ames, IA 50010, USA.
Sharma
Vijay K
VK
Carlson
Steve A
SA
D000818:Animals; D000831:Animals, Newborn; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000517:prevention & control / Q000628:therapy*; D015169:Colony Count, Microbial / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000254:growth & development*; D005243:Feces / Q000382:microbiology; D005260:Female; D007410:Intestinal Diseases / Q000382:microbiology / Q000517:prevention & control / Q000628:therapy / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000382:microbiology* / Q000517:prevention & control / Q000628:therapy*; D012486:Salmonella typhimurium / Q000254:growth & development*
D004269:DNA, Bacterial