[Diagnostic value of SYT-SSX fusion gene detection by fluorescence in-situ hybridization for synovial sarcoma].
OBJECTIVE
To establish a method of SYT-SSX fusion gene detection by FISH and to explore its diagnostic value for synovial sarcoma.


METHODS
The presence of SYT-SSX fusion gene was determined by FISH using a tissue microarray containing 62 known synovial sarcomas, 60 non-synovial sarcomas and 133 equivocal synovial sarcomas. FISH results were compared with those of RT-PCR published previously.


RESULTS
Overall, 96.9% (247/255) of the cases were successfully analyzed by FISH. The sensitivity of FISH for known synovial sarcomas was 96.7% (58/60), and the specificity for the non-synovial sarcoma was 100% (59/59). Moreover, SYT-SSX gene fusion was detected in 78.1% (100/128) of the equivocal synovial sarcomas. The concordance rate between FISH and RT-PCR was 83.6% (102/122) in those equivocal synovial sarcomas, and overall 79.7% (106/133) of these cases were confirmed as synovial sarcomas either by RT-PCR or by FISH.


CONCLUSIONS
The sensitivity and specificity of FISH detection of SYT-SSX fusion gene are high. FISH and RT-PCR are complementary to each other in the confirmation of synovial sarcomas, particularly those questionable cases.
Sun
Yan
Y
Department of Pathology, Tianjin Cancer Hospital, Tianjin 300060, China.
Sun
Bao-Cun
BC
Liu
Yan-Xue
YX
Zhang
Shi-Wu
SW
Zhao
Xiu-Lan
XL
Wang
Jian
J
Hao
Xi-Shan
XS
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000302:isolation & purification*; D057089:Pathology, Molecular / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013584:Sarcoma, Synovial / Q000175:diagnosis* / Q000235:genetics; D012983:Soft Tissue Neoplasms / Q000175:diagnosis / Q000235:genetics
D014408:Biomarkers, Tumor; D015514:Oncogene Proteins, Fusion; C116512:SYT-SSX fusion protein