An investigation of hormesis of trichloroethylene in L-02 liver cells by differential proteomic analysis.
Hormesis is the dose-response pattern of the biological responses to toxic chemicals, characterized by low-dose stimulation and high-dose inhibition. Although it is known that some cell types exhibit an adaptive response to low levels of cytotoxic agents, its molecular mechanism is still unclear and it has yet to be established whether this is a universal phenomenon that occurs in all cell types in response to exposure to every chemical. Trichloroethylene (TCE) is an organic solvent widely used and is released into the atmosphere from industrial degreasing operations. Acute (short-term) and chronic (long-term) inhalation exposure to trichloroethylene can affect the human health. In order to elucidate a cell-survival adaptive response of L-02 liver cells exposed to low dose of TCE, CCK-8 assay was used to assess cytotoxicity, and examined the possible mechanisms of hormesis by proteomics technology. We found that exposure of L-02 liver cells to low level of TCE resulted in adaptation to further exposure to higher level, about 1,000 protein-spots were obtained by two-dimensional electrophoresis (2-DE) and five protein spots were identified by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry sequencing of tryptic peptides. Our results suggest that a relationship may exist between identified proteins and TCE-induced hormesis, which are very useful for further study of the mechanism and risk assessment of TCE.
Huang
Hai-Yan
HY
Toxicology Laboratory, Shenzhen Center for Disease Control and Prevention, 518020 Shenzhen, Guangdong, People's Republic of China.
Liu
Jian-Jun
JJ
Xi
Ren-Rong
RR
Xing
Xiu-Mei
XM
Yuan
Jian-Hui
JH
Yang
Lin-Qing
LQ
Tao
Gong-Hua
GH
Gong
Chun-Mei
CM
Zhuang
Zhi-Xiong
ZX
D000704:Analysis of Variance; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014241:Trichloroethylene / Q000494:pharmacology*
D011506:Proteins; D020543:Proteome; D014241:Trichloroethylene; D005982:Glutathione Transferase