HIV-1 harboring renal tubular epithelial cell interaction with T cells results in T cell trans-infection.
Renal biopsy data suggest that renal tubular cells may serve as a reservoir for HIV-1, however the mechanism underlying this finding has not been studied. Here we show that primary human renal proximal tubular epithelial cells (HRPTECs) have the potential to harbor HIV-1 through the DEC-205 receptor. The interaction of HIV-1 with DEC-205 results in the rapid internalization of the virus for lysosomal degradation, without establishing a productive infection. However, a small fraction of incoming virus escapes degradation and can be rescued by T cells. Since pH-modulating agents and an inhibitor of endosomal transport increased HIV-1 accumulation and trans-infection to T cells, it appears that HRPTECs endocytic compartments may be the site of viral persistence and transmission to target cells. The ability of T cells to rescue the virus from HRPTECs further supports the hypothesis that these cells have the potential to serve as a reservoir for HIV-1.
Mikulak
Joanna
J
Department of Medicine, North Shore University Hospital and Long Island Jewish Medical Center, New Hyde Park, NY, USA.
Teichberg
Saul
S
Faust
Thomas
T
Schmidtmayerova
Helena
H
Singhal
Pravin C
PC
D000906:Antibodies / Q000502:physiology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology / Q000821:virology*; D015658:HIV Infections / Q000276:immunology / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007684:Kidney Tubules / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D053586:Virus Internalization
D000906:Antibodies; D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface