High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.
The direct separation of the enantiomers of four 2-aminomono- or dihydroxycyclopentanecarboxylic acids and four 2-aminodihydroxycyclohexanecarboxylic acids was performed on chiral stationary phases containing macrocyclic glycopeptide antibiotics such as teicoplanin (Astec Chirobiotic T and T2), teicoplanin aglycone (Chirobiotic TAG) or ristocetin A (Chirobiotic R) as chiral selectors. The effects of the nature of organic modifiers, the pH, the mobile phase composition and the structures of the analytes on the separation were investigated. Chirobiotic TAG, and in some cases Chirobiotic T, proved to be the most useful of these columns. The elution sequence was determined in most cases.
Berkecz
Róbert
R
Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, H-6720 Szeged, Hungary.
Ilisz
István
I
Benedek
Gabriella
G
Fülöp
Ferenc
F
Armstrong
Daniel W
DW
Péter
Antal
A
D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry / Q000302:isolation & purification*; D006020:Glycopeptides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012310:Ristocetin / Q000737:chemistry; D013237:Stereoisomerism; D017334:Teicoplanin / Q000031:analogs & derivatives / Q000737:chemistry
D002264:Carboxylic Acids; D003509:Cyclohexanecarboxylic Acids; D006020:Glycopeptides; D012310:Ristocetin; C016719:ristocetin A; D017334:Teicoplanin; C063101:teicoplanin aglycone