Correct charge state assignment of native electrospray spectra of protein complexes.
Correct charge state assignment is crucial to assigning an accurate mass to supramolecular complexes analyzed by electrospray mass spectrometry. Conventional charge state assignment techniques fall short of reliably and unambiguously predicting the correct charge state for many supramolecular complexes. We provide an explanation of the shortcomings of the conventional techniques and have developed a robust charge state assignment method that is applicable to all spectra.
Liepold
Lars
L
Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA.
Oltrogge
Luke M
LM
Suci
Peter A
PA
Young
Mark J
MJ
Douglas
Trevor
T
D000465:Algorithms; D008956:Models, Chemical; D046912:Multiprotein Complexes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D046912:Multiprotein Complexes; D011506:Proteins