Methylation and deamination of CpGs generate p53-binding sites on a genomic scale.
The formation of transcription-factor-binding sites is an important evolutionary process. Here, we show that methylation and deamination of CpG dinucleotides generate in vivo p53-binding sites in numerous Alu elements and in non-repetitive DNA in a species-specific manner. In light of this, we propose that the deamination of methylated CpGs constitutes a universal mechanism for de novo generation of various transcription-factor-binding sites in Alus.
Zemojtel
Tomasz
T
Department of Computational Molecular Biology, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany. zemojtel@molgen.mpg.de
Kielbasa
Szymon M
SM
Arndt
Peter F
PF
Chung
Ho-Ryun
HR
Vingron
Martin
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018899:CpG Islands / Q000502:physiology*; D019175:DNA Methylation; D003641:Deamination; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53