title: Effect of sodium chloride on establishment of callus and organogenesis in Brassica napus L.
abstract: In order to produce salt tolerant canola (Brassica napus L.) plants hypocotyl segments of its that were excised from 7-days-old-seedlings cultured in MS medium with various concentrations of PGRs (NAA, IBA, 2, 4-D, KN and BA) and sodium chloride. The best media for induction and growth of callus (6 mg L(-1) 2, 4-D and 2 mg L(-1) BA), production of adventitious shoots (1 mg L(-1) IBA and 1 mg L(-1) BA) and roots (1 mg L(-1) NAA and 0.5 mg L(-1) KN) were determined. Then explants cultured in these media plus 0-273.53 mM L(-1) sodium chloride. Explants in these media established and in some of concentrations of sodium chloride (68.37-102.56 mM L(-1)) produced calli and adventitious shoots and roots better than the same media but free from sodium chloride. This finding suggests that somaclonal variation can yield stable-tolerant canola plants.
authors.affiliation: Department of Plant Pathology, Iranian Research Institute of Plant Protection, No. 1 Yaman Ave, Chamran Exp'way, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Chamandoosti
mesh_terms: D000383:Agriculture / Q000379:methods*; D001901:Botany / Q000379:methods; D029688:Brassica napus / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018521:Plant Physiological Phenomena; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D000074262:Rapeseed Oil; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology*; D012987:Soil; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D010937:Plant Growth Regulators; D000074262:Rapeseed Oil; D012987:Soil; D012965:Sodium Chloride