title: Isolation, propagation, and characterization of human umbilical cord perivascular cells (HUCPVCs).
abstract: Current sources of mesenchymal cells, including bone marrow, fat and muscle, all require invasive procurement procedures, and provide relatively low frequencies of progenitors. Here, we describe the non-invasive isolation, and characterization, of a rich source of mesenchymal progenitor cells, which we call human umbilical cord perivascular cells (HUCPVCs). HUCPVCs show a similar immunological phenotype to bone marrow-derived mesenchymal stromal cells (BM-MSCs), since they are non-alloreactive, exhibit immunosuppression, and significantly reduce lymphocyte activation, in vitro. They present a non-hematopoietic myofibroblastic mesenchymal phenotype (CD45-, CD34-, CD105+, CD73+, CD90+, CD44+, CD106+, 3G5+, CD146+); with a 1:300 frequency at harvest, a short-doubling time, and a clonogenic frequency of >1:3 in culture. Furthermore, in addition to robust quinti-potential differentiation capacity in vitro, HUCPVCs have been shown to contribute to both musculo-skeletal and dermal wound healing in vivo.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Rahul
authors.initials: R
authors.lastname: Sarugaser
authors.forename: Jane
authors.initials: J
authors.lastname: Ennis
authors.forename: William L
authors.initials: WL
authors.lastname: Stanford
authors.forename: John E
authors.initials: JE
authors.lastname: Davies
mesh_terms: D000199:Actins / Q000378:metabolism; D001808:Blood Vessels / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002999:Clone Cells; D003893:Desmin / Q000378:metabolism; D004210:Dissection; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D009928:Organ Specificity; D014470:Umbilical Cord / Q000166:cytology*; D014746:Vimentin / Q000378:metabolism
chemical_list: D000199:Actins; D003893:Desmin; D014746:Vimentin