title: The Results of Mancini's test depend on the presence bound metal cations in the test protein.
abstract: Samples of human serum gamma-globulin with specifically bound copper or zinc cations were studied in Mancini's radial immunodiffusion test with human antibodies to IgG (H+L). The intensity of antibody binding to zinc-modified protein was 10-20% higher in comparison with the reference sample, while detection of gamma-globulin with bound copper by antibodies was 20-30% lower than in the corresponding reference sample. Comparison with the results of native gamma-globulin testing indicates limitations of Mancini's method as the quantitative assay for practical diagnosis, because under certain clinical conditions the traditional method can give over- and underestimated results.
authors.affiliation: Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. cheknev@riem.ru
authors.forename: S B
authors.initials: SB
authors.lastname: Cheknyov
authors.forename: E A
authors.initials: EA
authors.lastname: Denisova
authors.forename: E M
authors.initials: EM
authors.lastname: Mongush
authors.forename: Yu V
authors.initials: YV
authors.lastname: Shukhovtseva
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002412:Cations / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D009994:Osmolar Concentration; D011485:Protein Binding; D012698:Serologic Tests / Q000379:methods / Q000592:standards; D015032:Zinc / Q000737:chemistry / Q000378:metabolism; D005719:gamma-Globulins / Q000032:analysis* / Q000378:metabolism*
chemical_list: D002412:Cations; D008670:Metals; D005719:gamma-Globulins; D003300:Copper; D015032:Zinc