Type 1 and 2 IP3 receptors respond differently to catecholamines and stress.
Type 1 and 2 inositol 1,4,5-trisphosphate (IP3) receptors have been found in cardiac tissue, although they are localized in different types of cells. While the type 1 predominates in neuronal cells and cardiac ganglia, type 2 IP3 receptor is localized mainly in cardiomyocytes. In the heart, gene expression of the type 1 IP3 receptor is modulated by catecholamines, while type 2 is not affected. In this study we compared the effects of two stressors--cold exposure and hypoxia--on gene expression and protein levels of type 1 and 2 IP3 receptors. We found that both types of IP3 receptors were increased by these stressors, although the response of the type 2 IP3 receptor was delayed. We propose that regulation of these types of IP3 receptors in the heart might differ in control conditions and during stress.
Krizanova
Olga
O
Institute of Molecular Physiology and Genetics, Centre of Excellence for Cardiovascular Research SAS, Bratislava, Slovak Republic. olga.krizanova@savba.sk
Holotnakova
Tereza
T
Jurkovicova
Dana
D
Polakova
Eva
E
Zahradnikova
Alexandra
A
Lacinova
Lubica
L
Kvetnanský
Richard
R
Myslivecek
Jaromir
J
Pastorekova
Silvia
S
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002478:Cells, Cultured; D003080:Cold Temperature; D004299:Dopamine beta-Hydroxylase / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D000860:Hypoxia; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010656:Phenylephrine / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological
D000316:Adrenergic alpha-Agonists; D002395:Catecholamines; D053496:Inositol 1,4,5-Trisphosphate Receptors; D012333:RNA, Messenger; D010656:Phenylephrine; D004299:Dopamine beta-Hydroxylase