title: LCMS and GCMS for the screening of alkaloids in natural and in vitro extracts of Leucojum aestivum.
abstract: HPLC coupled to a mass spectrometer (MS) was used for the analysis of galanthamine and lycorine in natural extracts of Leucojum aestivum and in their in vitro cultures grown with a precursor (ACC), inhibitors (AgNO(3), STS), or an absorber (KMnO(4)) of ethylene. The maximum galanthamine (0.002%) and lycorine (0.02%) concentrations in tissue cultures were obtained in the presence of KMnO(4). GCMS was used to investigate underivatized alkaloid mixtures from L. aestivum. Seven alkaloids were identified in in vivo bulbs. KMnO(4) led to the highest diversity of alkaloids in tissue culture extracts.
authors.affiliation: Department of Plant Breeding and Seed Science, Agricultural University, 31-140 Krakow, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Ptak
authors.forename: Anna
authors.initials: A
authors.lastname: El Tahchy
authors.forename: François
authors.initials: F
authors.lastname: Dupire
authors.forename: Michel
authors.initials: M
authors.lastname: Boisbrun
authors.forename: Max
authors.initials: M
authors.lastname: Henry
authors.forename: Yves
authors.initials: Y
authors.lastname: Chapleur
authors.forename: Maria
authors.initials: M
authors.lastname: Moś
authors.forename: Dominique
authors.initials: D
authors.lastname: Laurain-Mattar
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D047151:Amaryllidaceae Alkaloids