Polyethylene glycol-conjugated immunoliposomes specific for olfactory ensheathing glial cells.
A novel immunoliposome delivery system was developed for directed transport into cultured olfactory epithelium cells. Monoclonal antibodies against glial fibrillary acidic protein (GFAP) served as a vector. Fluorescence microscopy showed that the target cells are specifically stained with Dil dye incorporated into liposomal membranes. This transport system holds promise for the delivery of bioactive substances to olfactory epithelial cells and modulation of their capacity to stimulate axonal regeneration.
Chekhonin
V P
VP
Laboratory of Immunochemistry, V. P. Serbskii State Research Center of Social and Forensic Psychiatry, Russian Ministry of Health, Russia. tsibylkina@gmail.com
Gurina
O I
OI
Ykhova
O V
OV
Ryabinina
A E
AE
Tsibulkina
E A
EA
Zhirkov
Yu A
YA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D000918:Antibody Specificity / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005260:Female; D005904:Glial Fibrillary Acidic Protein; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009186:Myelin Sheath / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009457:Neuroglia / Q000187:drug effects* / Q000276:immunology; D009831:Olfactory Mucosa / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D009832:Olfactory Nerve / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009928:Organ Specificity / Q000187:drug effects / Q000276:immunology; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D051381:Rats
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D008081:Liposomes; D009419:Nerve Tissue Proteins; C528900:glial fibrillary astrocytic protein, mouse; D011092:Polyethylene Glycols