Effect of Urtica dioica on morphometric indices of kidney in streptozotocin diabetic rats--a stereological study.
The aim of the present study was to investigate the effect of Urtica dioica on Morphometric indices of kidney in diabetic rats. Thirty male adult albino wistar rats of 125-175 g divided into control, diabetic and Urtica dioica treatment groups. In treatment Group, diabetic rats received 100 mg kg(-1) daily hydroalcoholic extract of U. dioica intraperitoneally for 4 weeks. After the animals had been sacrified, the kidneys were removed and fixed by formaldehyde, cut horizontally into 1 mm slices and processed, Stained with H and E. Stereological study performed using light microscope and the image projected on a table of olysa software. Cavalieri principle was used to estimate the volume of cortex, medulla and whole kidney. All the grouped data statistically evaluated using Student's t-test, expressed as the Mean +/- SE. Ration of kidney weight/body weight in diabetes (0.51) and diabetes-extract group (0.67) were higher then control group (0.42). Ratio of kidney volume/body weight in diabetes (350) and diabetes-extract group (348) were higher then control group (323). Volume Ratio of cortex/medulla in diabetes-extract group (1.65) was higher then control (1.34) and diabetes group (1.33). Glomerular area and diameter and proximal tubule diameter in diabetes-Extract group was higher than control and diabetes groups. This study revealed that Urtica dioica has no effect on renal morphometric indices in induced diabetic rats.
Golalipour
Mohammad Jafar
MJ
Department of Embryology and Histology, Gorgan Congenital Malformations Research Center, Gorgan University of Medical Sciences, P.O. Box 14165-553, Iran.
Gharravi
Anneh Mohammad
AM
Ghafari
Sorya
S
Afshar
Mohammad
M
D000818:Animals; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D015233:Models, Statistical; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D026982:Urtica dioica / Q000378:metabolism*
D010936:Plant Extracts