Sympathetic withdrawal augments cerebral blood flow during acute hypercapnia in sleeping lambs.
OBJECTIVE
Cerebral sympathetic activity constricts cerebral vessels and limits increases in cerebral blood flow (CBF), particularly in conditions such as hypercapnia which powerfully dilate cerebral vessels. As hypercapnia is common in sleep, especially in sleep disordered breathing, we tested the hypothesis that sympathetic innervation to the cerebral circulation attenuates the CBF increase that accompanies increases in PaCO2 in sleep, particularly in REM sleep when CBF is high.


METHODS
Newborn lambs (n = 5) were instrumented to record CBF, arterial pressure (AP) intracranial pressure (ICP), and sleep-wake state (quiet wakefulness (QW), NREM, and REM sleep). Cerebral vascular resistance was calculated as CVR = [AP-ICP]/CBF. Lambs were subjected to 60-sec tests of hypercapnia (FICO2 = 0.08) during spontaneous sleep-wake states before (intact) and after sympathectomy (bilateral superior cervical ganglionectomy).


RESULTS
During hypercapnia in intact animals, CBF increased and CVR decreased in all sleep-wake states, with the greatest changes occurring in REM (CBF 39.3% +/- 6.1%, CVR -26.9% +/- 3.6%, P < 0.05). After sympathectomy, CBF increases (26.5% +/- 3.6%) and CVR decreases (-21.8% +/- 2.1%) during REM were less (P < 0.05). However the maximal CBF (27.8 +/- 4.2 mL/min) and minimum CVR (1.8 +/- 0.3 mm Hg/ min/mL) reached during hypercapnia were similar to intact values.


CONCLUSIONS
Hypercapnia increases CBF in sleep and wakefulness, with the increase being greatest in REM. Sympathectomy increases baseline CBF, but decreases the response to hypercapnia. These findings suggest that cerebral sympathetic nerve activity is normally withdrawn during hypercapnia in REM sleep, augmenting the CBF response.
Cassaglia
Priscila A
PA
Ritchie Centre for Baby Health Research, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia.
Griffiths
Robert I
RI
Walker
Adrian M
AM
D000818:Animals; D000831:Animals, Newborn; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D001921:Brain / Q000098:blood supply*; D006935:Hypercapnia / Q000503:physiopathology*; D010100:Oxygen / Q000097:blood; D012756:Sheep; D012894:Sleep Stages / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology; D013562:Sympathectomy; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D014655:Vascular Resistance / Q000502:physiology; D014664:Vasodilation / Q000502:physiology*
D010100:Oxygen