[Intracellular clearance of Coxsakievirus B3 infection by short interfering RNA and its mechanism study].
OBJECTIVE
To evaluate the possibility of short interfering RNA (siRNA) inhibiting Coxsackievirus B3 (CVB3) infection in vitro, and discover the mechanism initially.


METHODS
We obtained proper effective dosage of siRNA by observing cytopathic effect (CPE). Estimate its antiviral activities and its pathway of siRNA by Western Blot assay and RT-PCR.


RESULTS
Results showed that siRNA-3753 can be effectively transfected into HeLa cells, we can achieve a high transfection efficiency up to 98.77% and its effect can last for 48 h stably in cells. 0.6 micromol/L siRNA-3753 got a high inhibiting effect of virus and didn't show any toxicity to cells. So we consider this concentration as the experimental concentration. siRNA-3753 can debase virus reproduction. The antiviral effect is sequence-specific and is not attributable to either interferon or the interferon response effectors protein kinase R (PKR).


CONCLUSIONS
The data confirmed that siRNA can effectively inhibit CVB3 infection in vitro, its antivirus effect was gained from specific debase of virus genome.
Xiao
Zong-Hui
ZH
Capital Institute of Pediatrics, Beijing 100020, China.
Han
Ji-Sheng
JS
Yao
Hai-Lan
HL
Liu
Zhe-Wei
ZW
D003384:Coxsackievirus Infections / Q000628:therapy* / Q000821:virology; D029822:Enterovirus B, Human / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*; D012367:RNA, Viral / Q000235:genetics
D034741:RNA, Small Interfering; D012367:RNA, Viral