Hyperphosphatemia-induced hyperparathyroidism in 5/6 nephrectomized rats: development of a new animal model.
BACKGROUND
We require a stable model to understand the molecular mechanism by which isolated hyperphosphatemia induces hyperparathyroidism secondary to chronic renal failure. The present study established a rat model of hyperphosphatemia-induced secondary hyperparathyroidism in chronic renal failure.


METHODS
Twenty-nine rats with 5/6 nephrectomy (Nx) were divided into three groups and were fed for 10 weeks on a high phosphate diet (1.2% phosphate) starting from three different post-Nx time points. Parathyroid hormone mRNA in parathyroid gland was measured by real-time PCR and parathyroid cell hyperplasia was tested by proliferating cell nuclear antigen (PCNA) assay.


RESULTS
The 10 rats fed a high phosphate diet starting from the fourth week post-Nx had isolated hyperphosphatemia and excess synthesis/secretion of parathyroid hormone, and hyperplasia of the parathyroid glands were induced (r = 0.86 - 0.97, P < 0.001), but the levels of serum calcium and 1, 25 (OH)2D3 did not change.


CONCLUSIONS
A rat model of hyperphosphatemia-induced secondary hyperparathyroidism in chronic renal failure was established by 5/6 Nx and 10 weeks-high phosphate diet starting from the fourth week post-Nx.
Jiang
Ying
Y
Department of Nephrology, Peking University People's Hospital, Beijing 100044, China.
Wang
Mei
M
D000818:Animals; D004195:Disease Models, Animal; D006961:Hyperparathyroidism / Q000209:etiology*; D054559:Hyperphosphatemia / Q000150:complications*; D007676:Kidney Failure, Chronic / Q000150:complications; D008297:Male; D009392:Nephrectomy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar