title: Direct production of L-tagatose from L-psicose by Enterobacter aerogenes 230S.
abstract: L-tagatose was produced directly from L-psicose by subjecting the same biomass suspension to microbial reduction followed by oxidation using a newly isolated bacteria Enterobacter aerogenes 230S. After various optimizations, it was observed that cells grown on xylitol have the best conversion potential. Moreover, E. aerogenes 230S converted L-psicose to L-tagatose at a faster rate in the presence of polyols such as glycerol, D-sorbitol, ribitol, L-arabitol, D-mannitol and xylitol. At 5% substrate concentration, the conversion ratio of L-psicose to L-tagatose was above 60% in the presence of glycerol. Identity of crystalline L-tagatose was confirmed by HPLC analysis, (13)C-NMR spectra, and optical rotation.
authors.affiliation: Rare Sugar Research Center, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, Japan.
authors.forename: Devendar
authors.initials: D
authors.lastname: Rao
authors.forename: Pushpakiran
authors.initials: P
authors.lastname: Gullapalli
authors.forename: Akihide
authors.initials: A
authors.lastname: Yoshihara
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Jenkinson
authors.forename: Kenji
authors.initials: K
authors.lastname: Morimoto
authors.forename: Goro
authors.initials: G
authors.lastname: Takata
authors.forename: Kazuya
authors.initials: K
authors.lastname: Akimitsu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tajima
authors.forename: George W J
authors.initials: GW
authors.lastname: Fleet
authors.forename: Ken
authors.initials: K
authors.lastname: Izumori
mesh_terms: D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D021902:Enterobacter aerogenes / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D013402:Sugar Alcohols / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D006601:Hexoses; D013402:Sugar Alcohols; C003243:psicose; D005632:Fructose; C042890:altritol; D002244:Carbon; D010100:Oxygen; C030192:tagatose