title: Visible-light photocontrol of (E)/(Z) isomerization of the 4-(dimethylamino)azobenzene pseudo-nucleotide unit incorporated into an oligonucleotide and DNA hybridization in aqueous media.
abstract: We demonstrate significant photoresponsivity in aqueous media to visible light of pseudo-oligonucleotides possessing 4-(dimethylamino)azobenzene (4-DMAzo) side chains. The spectrum of the 4-DMAzo moiety during 436 nm light irradiation at pH >9 was clearly different from that of the all (E)-form, indicating the presence of the (Z)-form. Thermal (Z)-to-(E) recovery isomerization was faster at pH 9 (k(Z)(-E) = approximately 10(1) s(-1)) than at pH 11; however, addition of 50% ethanol significantly slowed the thermal recovery isomerization at pH 9 (k(Z)(-E) = approximately 2 s(-1)) and increased the magnitude of the spectral changes. Significant photoregulation of DNA hybridization by visible light was demonstrated under this condition.
authors.affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan. kameitakashi@es.hokudai.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamei
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Akiyama
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Morii
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tamaoki
authors.forename: Taro Q P
authors.initials: TQ
authors.lastname: Uyeda
mesh_terms: D002021:Buffers; D004247:DNA / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008027:Light; D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry; D004124:p-Dimethylaminoazobenzene / Q000737:chemistry*
chemical_list: D002021:Buffers; D009841:Oligonucleotides; D014867:Water; D000431:Ethanol; D004247:DNA; D004124:p-Dimethylaminoazobenzene