In vitro differentiation of human cord blood-derived unrestricted somatic stem cells into hepatocyte-like cells on poly(epsilon-caprolactone) nanofiber scaffolds.
Tissue engineering of implantable cellular constructs is an emerging cellular therapy for hepatic disease. In this study, we tested the ability of poly(epsilon-caprolactone) (PCL) nanofiber scaffold to support and maintain hepatic differentiation of human cord blood-derived unrestricted somatic stem cells (USSCs) in vitro. USSCs, self-renewing pluripotent cells, were isolated from human cord blood. The electrospun PCL nanofiber porous scaffold was constructed of uniform, randomly oriented nanofibers. USSCs were seeded onto PCL nanofiber scaffolds, and were induced to differentiate into hepatogenic lineages by culturing with differentiation factors for 6 weeks. RT-PCR analysis of endoderm and hepatic-specific gene expression, immunohistochemical detection of cytokeratin 18 (CK-18), alpha-fetoprotein, albumin, glycogen storage and indocyanine green uptake confirmed the differentiation of USSCs into endoderm and hepatocyte-like cells. In the present study, we show that hepatocyte-like cells differentiated from USSCs on the PCL nanofiber scaffold can be candidate for tissue engineering and cell therapy of hepatic tissues.
Hashemi
Seyed Mahmoud
SM
Department of Stem Cell and Tissue Engineering, Stem Cell Technology Co. Ltd., Tehran, Iran.
Soleimani
Masoud
M
Zargarian
Seyed Shahrooz
SS
Haddadi-Asl
Vahid
V
Ahmadbeigi
Naser
N
Soudi
Sara
S
Gheisari
Yousof
Y
Hajarizadeh
Athena
A
Mohammadi
Yousef
Y
D002208:Caproates / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004867:Equipment Design; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression; D006003:Glycogen / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007208:Indocyanine Green / Q000378:metabolism; D007783:Lactones / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D002208:Caproates; D007783:Lactones; D011108:Polymers; C121056:caprolactone; D006003:Glycogen; D007208:Indocyanine Green