Inhibition of human cervical carcinoma growth by cytokine-induced killer cells in nude mouse xenograft model.
Cervical cancer is a major cause of cancer mortality in women worldwide and is an important public health problem for adult women in developing countries. Despite aggressive treatment with surgery and chemoradiation, the outcomes for cervical cancer patients remain poor. In this study, the antitumor activity of cytokine-induced killer (CIK) cells against human cervical cancer was evaluated in vitro and in vivo. Human peripheral blood mononuclear cells were cultured with IL-2-containing medium in anti-CD3 antibody-coated flasks for 5 days, followed by incubation in IL-2-containing medium for 9 days. The resulting populations of CIK cells comprised 95% CD3(+), 3% CD3(-)CD56(+), 35% CD3(+)CD56(+), 11% CD4(+), <1% CD4(+)CD56(+), 80% CD8(+), and 25% CD8(+)CD56(+). At an effector-target cell ratio of 100:1, CIK cells destroyed 56% of KB-3-1 human cervical cancer cells, as measured by the (51)Cr-release assay. In addition, CIK cells at doses of 3 and 10 million cells per mouse inhibited 34% and 57% of KB-3-1 tumor growth in nude mouse xenograft assays, respectively. This study suggests that CIK cells may be used as an adoptive immunotherapy for cervical cancer patients.
Kim
Hwan Mook
HM
Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungbuk 363-883, Republic of Korea.
Lim
Jaeseung
J
Kang
Jong Soon
JS
Park
Song-Kyu
SK
Lee
Kiho
K
Kim
Jee Youn
JY
Kim
Yeon Jin
YJ
Hong
Jin Tae
JT
Kim
Youngsoo
Y
Han
Sang-Bae
SB
D000818:Animals; D002277:Carcinoma / Q000276:immunology / Q000628:therapy*; D045744:Cell Line, Tumor; D055612:Cytokine-Induced Killer Cells / Q000187:drug effects / Q000276:immunology / Q000637:transplantation*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
D007376:Interleukin-2