Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR.
Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.
Wu
Josephine
J
Department of Pathology, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1122, New York, New York 10029, USA.
Zhang
Wandi
W
Xie
Boxun
B
Wu
Maoxin
M
Tong
Xiaodi
X
Kalpoe
Jayant
J
Zhang
David
D
D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D003015:Clostridium Infections / Q000175:diagnosis*; D003016:Clostridium perfringens / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005404:Fixatives / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014021:Tissue Preservation / Q000379:methods*
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005404:Fixatives; D012336:RNA, Ribosomal, 16S; D005557:Formaldehyde