[FLASH-PCR diagnostics of toxigenic fungi of the genus Fusarium].
A test system for the diagnostics and identification of seven toxigenic fungi causing fusarioses of cereals (Fusarium graminearum, F. culmorum, F. poae, F. sporotrichioides, F. langsethiae, F. avenaceum, and F. tricinctum) was developed using PCR. The identification of pathogen is based on the specific amplification of a DNA fragment of the gene of translation elongation factor 1 alpha (tef-1alpha) and subsequent detection of the results by the fluorescent amplification-based specific hybridization method. The system was tested on 38 isolates of different fungi of the genus Fusarium.
Riazantsev
D Iu
DIu
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia.
Abramova
S L
SL
Evstratova
S V
SV
Gagkaeva
T Iu
TIu
Zavriev
S K
SK
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D005670:Fusarium / Q000737:chemistry / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004271:DNA, Fungal