Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria.
Acquisition of knowledge of the nature and DNA content of B chromosomes has been triggered by a collection of molecular techniques, one of which, microdissection, has provided interesting results in a number of B chromosome systems. Here we provide the first data on the molecular composition of B chromosomes in Locusta migratoria, after microdissection of the B and X chromosomes, DNA amplification by one (B) or two (X) different methods, and chromosome painting. The results showed that B chromosomes share at least two types of repetitive DNA sequences with the A chromosomes, suggesting that Bs in this species most likely arose intraspecifically. One of these repetitive DNAs is located on the heterochromatic distal half of the B chromosome and in the pericentromeric regions of about half of the A chromosomes, including the X. The other type of repetitive DNA is located interspersedly over the non-centromeric euchromatic regions of all A chromosomes and in an interstitial part of the proximal euchromatic half of the B chromosome. Chromosome painting, however, did not provide results sufficiently reliable to determine, in this species, which A chromosome gave rise to the B; this might be done by detailed analysis of the microdissected DNA sequences.
Teruel
María
M
Departamento de Genética, Universidad de Granada, 18071, Granada, Spain.
Cabrero
Josefa
J
Montiel
Eugenia E
EE
Acosta
Manuel J
MJ
Sánchez
Antonio
A
Camacho
Juan Pedro M
JP
D000818:Animals; D020223:Chromosome Painting; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D048188:Locusta migratoria / Q000235:genetics*; D042282:Microdissection; D049471:Pachytene Stage; D014960:X Chromosome / Q000235:genetics* / Q000648:ultrastructure
D004247:DNA