title: Additional amplifications of SERS via an optofluidic CD-based platform.
abstract: In this paper, signal amplifications of surface-enhanced Raman scattering (SERS) are realized by an optofluidic compact disc (CD)-based preconcentration method for effective label-free environmental and biomolecular detections. The preconcentration of target molecules is accomplished through the accumulation of adsorbed molecules on SERS-active sites by repeating a 'filling-drying' cycle of the assay solution in the optofluidic CD platform. After 30 cycles, the clear and high SERS signal of rhodamine 6G of 1 nM is readily detected. In addition to the preconcentration-based signal amplification by the optofluidic SERS system on the CD platform, we introduce a controlled precipitation of gold nanoparticles by CuSO4 for SERS substrates. This method provides high-throughput, high-sensitive and large-area uniform SERS substrates on the optofluidic CD platform. The uniform SERS signals from different positions in spots of 3 mm2 on the different CDs gives us confidence in the reliability and stability of our SERS substrates.
authors.affiliation: Biomolecular Nanotechnology Center, Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California at Berkeley, Berkeley, California 94720, USA.
authors.forename: Dukhyun
authors.initials: D
authors.lastname: Choi
authors.forename: Taewook
authors.initials: T
authors.lastname: Kang
authors.forename: Hansang
authors.initials: H
authors.lastname: Cho
authors.forename: Yeonho
authors.initials: Y
authors.lastname: Choi
authors.forename: Luke P
authors.initials: LP
authors.lastname: Lee
mesh_terms: D016250:Compact Disks; D019327:Copper Sulfate / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D006046:Gold; D019327:Copper Sulfate