Gas-phase H/D exchange and collision cross sections of hemoglobin monomers, dimers, and tetramers.
The conformations of gas-phase ions of hemoglobin, and its dimer and monomer subunits have been studied with H/D exchange and cross section measurements. During the H/D exchange measurements, tetramers undergo slow dissociation to dimers, and dimers to monomers, but this did not prevent drawing conclusions about the relative exchange levels of monomers, dimers, and tetramers. Assembly of the monomers into tetramers, hexamers, and octamers causes the monomers to exchange a greater fraction of their hydrogens. Dimer ions, however, exchange a lower fraction of their hydrogens than monomers or tetramers. Solvation of tetramers affects the exchange kinetics. Solvation molecules do not appear to exchange, and solvation lowers the overall exchange level of the tetramers. Cross section measurements show that monomer ions in low charge states, and tetramer ions have compact structures, comparable in size to the native conformations in solution. Dimers have remarkably compact structures, considerably smaller than the native conformation in solution and smaller than might be expected from the monomer or tetramer cross sections. This is consistent with the relatively low level of exchange of the dimers.
Wright
P John
PJ
Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada.
Douglas
D J
DJ
D000818:Animals; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D055503:Protein Multimerization; D014867:Water / Q000737:chemistry
D006454:Hemoglobins; D014867:Water; D006859:Hydrogen