[Transciptome among Mexicans: a large scale methodology to analyze the genetics expression profile of simultaneous samples in muscle, adipose tissue and lymphocytes obtained from the same individual].
OBJECTIVE
We describe the methodology used to analyze multiple transcripts using microarray techniques in simultaneous biopsies of muscle, adipose tissue and lymphocytes obtained from the same individual as part of the standard protocol of the Genetics of Metabolic Diseases in Mexico: GEMM Family Study.


METHODS
We recruited 4 healthy male subjects with BM1 20-41, who signed an informed consent letter. Subjects participated in a clinical examination that included anthropometric and body composition measurements, muscle biopsies (vastus lateralis) subcutaneous fat biopsies anda blood draw. All samples provided sufficient amplified RNA for microarray analysis. Total RNA was extracted from the biopsy samples and amplified for analysis.


RESULTS
Of the 48,687 transcript targets queried, 39.4% were detectable in a least one of the studied tissues. Leptin was not detectable in lymphocytes, weakly expressed in muscle, but overexpressed and highly correlated with BMI in subcutaneous fat. Another example was GLUT4, which was detectable only in muscle and not correlated with BMI. Expression level concordance was 0.7 (p< 0.001) for the three tissues studied.


CONCLUSIONS
We demonstrated the feasibility of carrying out simultaneous analysis of gene expression in multiple tissues, concordance of genetic expression in different tissues, and obtained confidence that this method corroborates the expected biological relationships among LEPand GLUT4. TheGEMM study will provide a broad and valuable overview on metabolic diseases, including obesity and type 2 diabetes.
Bastarrachea
Raúl A
RA
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX, USA. raulbs@sfbrgenetics.org
López-Alvarenga
Juan Carlos
JC
Kent
Jack W
JW
Laviada-Molina
Hugo A
HA
Cerda-Flores
Ricardo M
RM
Calderón-Garcidueñas
Ana Laura
AL
Torres-Salazar
Amada
A
Torres-Salazar
Amanda
A
Nava-González
Edna J
EJ
Solis-Pérez
Elizabeth
E
Gallegos-Cabrales
Esther C
EC
Cole
Shelley A
SA
Comuzzie
Anthony G
AG
D000328:Adult; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008297:Male; D008800:Mexico; D018482:Muscle, Skeletal / Q000737:chemistry; D012313:RNA / Q000032:analysis; D050151:Subcutaneous Fat / Q000737:chemistry
D012313:RNA