title: Association of parental hyperhomocysteinemia and C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism with recurrent pregnancy loss.
abstract: OBJECTIVE
  To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL).
  
  
  METHODS
  A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to assess total plasma homocysteine, C677T MTHFR polymorphism and DNA damage respectively. Student t-test, ANOVA and Fisher exact test were used for statistical analysis.
  
  
  RESULTS
  Maternal [mean: 11.6+/-5.0 versus 8.6+/-4.2 micromol/L, odds ratio (OR): 4.48] and paternal [mean: 19.6+/-9.5 versus 14.2+/-7.4 micromol/L, OR: 6.92] hyperhomocysteinemia, paternal age [OR: 1.16], paternal MTHFR 677T allele [OR: 2.30] and DNA damage were found to increase the risk for RPL. DNA damage showed positive correlation with plasma homocysteine and MTHFR 677T allele.
  
  
  CONCLUSIONS
  Parental hyperhomocysteinemia, paternal age, paternal C677T MTHFR polymorphism and DNA damage are risk factors for RPL. DNA damage showed positive correlation with plasma homocysteine and MTHFR 677T allele.
authors.affiliation: Dept. of pathology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Vinukonda
authors.initials: V
authors.lastname: Govindaiah
authors.forename: Shaik Mohammad
authors.initials: SM
authors.lastname: Naushad
authors.forename: Krishnamurthy
authors.initials: K
authors.lastname: Prabhakara
authors.forename: Prasad Chintakindi
authors.initials: PC
authors.lastname: Krishna
authors.forename: Akella
authors.initials: A
authors.lastname: Radha Rama Devi
mesh_terms: D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution / Q000235:genetics; D016022:Case-Control Studies; D004249:DNA Damage; D020964:Embryo Loss / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006710:Homocysteine / Q000097:blood; D006801:Humans; D020138:Hyperhomocysteinemia / Q000201:enzymology* / Q000235:genetics*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D048629:Micronuclei, Chromosome-Defective; D010290:Parents; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D012008:Recurrence; D055815:Young Adult
chemical_list: D006710:Homocysteine; D042965:Methylenetetrahydrofolate Reductase (NADPH2)