Establishment of conditionally immortalized epithelial cell lines from the intestinal tissue of adult normal and transgenic mice.
It has proved to be impossible to culture epithelial cells from the gastrointestinal tract of adult animals. Researchers have had to use either cell lines derived from newborn rat small intestine or colon carcinoma cell lines that have retained some of the properties of the gastrointestinal mucosa. We have described a method for establishing conditionally immortalized cell lines from the stomach, small intestine, colon, pancreas, and liver from tissue obtained from a transgenic mouse strain carrying a temperature-sensitive mutant of the SV40 large T gene (the "Immortomouse"). This immortalizing gene has proved to be useful for establishing cell lines from a number of transgenic mice following crossbreeding of the Immortomouse with the transgenic mouse of interest. These cell lines are being used in numerous studies. In this review we describe the methods for developing such lines and list the range of cell lines that have been developed from colon, small intestine, stomach, liver, and pancreas of a number of transgenic mice.
Whitehead
Robert H
RH
Novel Cell Line Core Facility, Vanderbilt Digestive Disease Research Center, MRB IV, Rm. 1055, Vanderbilt University, Nashville, TN 37232, USA. robert.whitehead@vanderbilt.edu
Robinson
Pamela S
PS
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D018929:Cell Culture Techniques / Q000379:methods*; D002461:Cell Line, Transformed; D004847:Epithelial Cells / Q000166:cytology*; D007413:Intestinal Mucosa / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic
D000952:Antigens, Polyomavirus Transforming