Serum of patients with septic shock stimulates the expression of Trem-1 on U937 monocytes.
OBJECTIVE
To describe the concentrations of sTREM-1 in patients with sepsis and to explore the effects of their serum on the expression of TREM-1 on U937 monocytes.


METHODS
Blood was sampled at regular time intervals in 56 patients with sepsis. Concentrations of tumour necrosis factor-alpha (TNFalpha), interleukin-1beta (IL-1alpha), IL-6, IL-8, IL-10 and IL-12p70 and sTREM-1 were measured. U937 monocytes were incubated in the presence of serum at sepsis onset.


RESULTS
Median sTREM-1 concentration on day 1 for patients with septic shock was 915 pg/ml and 228.5 pg/ml for those without shock (p = 0.002). TNFalpha, IL-1alpha, IL-6, IL-8 and IL-10 did not differ between them. A positive correlation was found between changes of sTREM-1 and SOFA scores from day 1 to 7. Sera of patients with septic shock evoked a significant increase of the expression of TREM-1. The concentrations of TNFalpha and IL-8 in supernatants increased only after stimulating with sera of patients without shock, but not after stimulating with sera of patients with shock.


CONCLUSIONS
Levels of sTREM-1 correlated with sepsis severity. sTREM-1 is considerably higher in patients with shock compared to patients without shock. The serum of shocked patients could stimulate the expression of TREM-1 on U937 monocytes.
Dimopoulou
I
I
2nd Department of Critical Care Medicine, University of Athens, Greece.
Orfanos
S E
SE
Pelekanou
A
A
Kotanidou
A
A
Livaditi
O
O
Augustatou
C
C
Zervou
M
M
Douka
E
E
Theodorakopoulou
M
M
Karagianni
V
V
Douzinas
E
E
Armaganidis
A
A
Giamarellos-Bourboulis
E J
EJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D012720:Severity of Illness Index; D012772:Shock, Septic / Q000097:blood / Q000276:immunology; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
D016207:Cytokines; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1