Proteomic analysis of cerebrospinal fluid from patients with idiopathic temporal lobe epilepsy.
Proteomic analysis of cerebrospinal fluid (CSF) from patients with temporal lobe epilepsy (TLE) and controls was carried out using two-dimensional gel electrophoresis followed by liquid chromatography electrospray ionization tandem mass spectrometry. Five protein spots showed significant differential expression (p<0.05): vitamin D-binding protein (DBP) was elevated in the CSF of TLE patients whereas cathepsin D, apolipoprotein J, Fam3c, and superoxide dismutase 1 (SOD1) were decreased in the CSF of TLE patients. Additional six protein spots presented only in the CSF of epilepsy patients were identified as tetranectin (TN), talin-2, apolipoprotein E, immunoglobulin lambda light chain (IGL@), immunoglobulin kappa variable light chain 1-5 (IGKV1-5), and procollagen C-endopeptidase enhancer 1 (PCOLCE). Expression of DBP, SOD1 and talin-2 was validated by western blot. Our results may provide better understanding of the pathophysiologic mechanisms underlying epileptogenesis and possible epilepsy biomarkers.
Xiao
Fei
F
Neurology Department, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1st You Yi Road, Chongqing 400016, China.
Chen
Dan
D
Lu
Yang
Y
Xiao
Zheng
Z
Guan
Li-feng
LF
Yuan
Jie
J
Wang
Liang
L
Xi
Zhi-qin
ZQ
Wang
Xue-feng
XF
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D002402:Cathepsin D / Q000134:cerebrospinal fluid; D002555:Cerebrospinal Fluid / Q000737:chemistry; D002853:Chromatography, Liquid; D051152:Clusterin / Q000134:cerebrospinal fluid; D016207:Cytokines / Q000134:cerebrospinal fluid; D015180:Electrophoresis, Gel, Two-Dimensional; D004833:Epilepsy, Temporal Lobe / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000134:cerebrospinal fluid; D040901:Proteomics / Q000379:methods; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid*; D000072105:Superoxide Dismutase-1; D016608:Talin / Q000134:cerebrospinal fluid*; D014809:Vitamin D-Binding Protein / Q000134:cerebrospinal fluid*; D055815:Young Adult
D015415:Biomarkers; D051152:Clusterin; D016207:Cytokines; C514209:FAM3C protein, human; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; C000606290:SOD1 protein, human; C436513:TLN2 protein, human; D016608:Talin; D014809:Vitamin D-Binding Protein; C048648:tetranectin; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D002402:Cathepsin D