Fingerprint analysis of anti-tumor active polypeptides from Arca subcrenata by HPLC.
RP-HPLC was applied to analyze active polypeptides in Arca subcrenata, and the optimal condition for separation was also set up: temperature: 30 degrees C; wavelength: 280 nm; flow rate: 1.0 ml/min; Solvent A consisted of 80% acetonitrile and 0.1% trifluoroacetic acid (TFA) and solvent B contained 0.1% TFA. In this condition, ten samples' fingerprints were gained, in one of which the genuine fraction exhibited fourteen "common peaks" representing the characteristics of the constituents.
Ren
Sheng-fang
SF
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Song
Li-yan
LY
Yan
Chun-yan
CY
Li
Ting-fei
TF
Zhao
Yu
Y
Yu
Rong-min
RM
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D049872:Bivalvia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D013696:Temperature
D000097:Acetonitriles; D000970:Antineoplastic Agents; D010455:Peptides; D012997:Solvents