title: Toward prevention of cowdriosis.
abstract: Mass production of Ehrlichia ruminantium variants from different regions of sub-Saharan Africa is one of the difficulties that must be overcome in producing a heartwater vaccine. Vaccine productivity can be limited by endogenous induction of interferon (IFN), which inhibits the propagation of Ehrlichia ruminantium (ER) in cell culture. Different kinds of endothelial cells, in which ER multiply efficiently, could be grown in a scalable way in VueLife Teflon bags on Cytodex 3 microcarriers where bead-to-bead transfer of cells occurs. The digital holographic microscope designed at the Université Libre de Bruxelles allows detection of the most appropriate time to harvest intracellular microorganisms for vaccine production.
authors.affiliation: Animal Cell Biotechnology, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Andreia
authors.initials: A
authors.lastname: Pedregal
authors.forename: Daniel Ribeiro
authors.initials: DR
authors.lastname: de Sousa
authors.forename: Hoang Nam
authors.initials: HN
authors.lastname: Nguyen
authors.forename: Eane Agapito
authors.initials: EA
authors.lastname: das Neves
authors.forename: Serge
authors.initials: S
authors.lastname: Lowagie
authors.forename: Thierry
authors.initials: T
authors.lastname: Marique
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kagye
authors.forename: Ines Teixeira
authors.initials: IT
authors.lastname: Guerra
authors.forename: Yvette
authors.initials: Y
authors.lastname: Kamba
authors.forename: Philippe
authors.initials: P
authors.lastname: Totte
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vachiéry
authors.forename: Thierry
authors.initials: T
authors.lastname: Lefrançois
authors.forename: Dominique
authors.initials: D
authors.lastname: Martinez
authors.forename: Catherine
authors.initials: C
authors.lastname: Yourassowsky
authors.forename: Natacha
authors.initials: N
authors.lastname: Callens
authors.forename: Olivier
authors.initials: O
authors.lastname: Monnom
authors.forename: Frank
authors.initials: F
authors.lastname: Dubois
authors.forename: John
authors.initials: J
authors.lastname: Wérenne
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002417:Cattle; D016873:Ehrlichiosis / Q000517:prevention & control*; D006801:Humans; D006822:Hybrid Cells; D007372:Interferons / Q000378:metabolism*
chemical_list: D001428:Bacterial Vaccines; D007372:Interferons