Upstream-binding factor is sequestered into herpes simplex virus type 1 replication compartments.
Previous reports have shown that adenovirus recruits nucleolar protein upstream-binding factor (UBF) into adenovirus DNA replication centres. Here, we report that despite having a different mode of viral DNA replication, herpes simplex virus type 1 (HSV-1) also recruits UBF into viral DNA replication centres. Moreover, as with adenovirus, enhanced green fluorescent protein-tagged fusion proteins of UBF inhibit viral DNA replication. We propose that UBF is recruited to the replication compartments to aid replication of HSV-1 DNA. In addition, this is a further example of the role of nucleolar components in viral life cycles.
Stow
Nigel D
ND
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Evans
Vanessa C
VC
Matthews
David A
DA
D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D035463:Pol1 Transcription Initiation Complex Proteins / Q000378:metabolism*; D014779:Virus Replication
D004279:DNA, Viral; D035463:Pol1 Transcription Initiation Complex Proteins; C063844:transcription factor UBF