title: [Effects of resveratrol on the release of Glu and Gln in cultured rat astrocytes with stretch injury].
abstract: OBJECTIVE
  To study the effects of resveratrol on amino acid release in cultured astrocytes with stretch injury and explore its possible mechanism for central nervous system protection.
  
  
  METHODS
  Cultured rat astrocytes were treated with different concentrations of resveratrol for 12 h and the subsequent changes in Glu and Gln release and lactate dehydrogenase(LDH) leakage were examined after stretch injury.
  
  
  RESULTS
  High-performance liquid chromatography showed that stretch injury increased Glu release from the astrocytes (P<0.05), and at the concentration of 1 micromol/L, resveratrol further increased Glu release (P<0.05), whereas at 100 micromol/L, resveratrol obviously inhibit Glu release (P<0.05). LDH leakage increased significantly after stretch injury of the astrocytes (P<0.05), and resveratrol acted to further increase LDH leakage at 1 micromol/L but inhibited LDH leakage at 100 micromol/L (P<0.05).
  
  
  CONCLUSIONS
  Resveratrol can inhibit the release of Glu and LDH leakage from rat astrocytes to protect the cells from stretch injury.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. zhou_hao120@163.com
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Jiang
authors.initials: J
authors.lastname: Guo
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dan-li
authors.initials: DL
authors.lastname: Kong
authors.forename: Shou-yi
authors.initials: SY
authors.lastname: Yu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D005973:Glutamine / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013314:Stress, Mechanical
chemical_list: D018696:Neuroprotective Agents; D013267:Stilbenes; D005973:Glutamine; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D000077185:Resveratrol