title: Evaluation of a Microbiological Multi-Residue System on the detection of antibacterial substances in ewe milk.
abstract: To protect both, public health and the dairy industry, from the presence of antibiotic residues in milk, control programmes have been established, which include the needed screening tests. This work focuses on the application of a Microbiological Multi-Residue System in ewe milk, a method based on the use of six different plates, each seeded with one of the following bacteria: Geobacillus stearothermophilus var. calidolactis (beta-lactams), Bacillus subtilis at pH 8.0 (aminoglycosides), Kocuria rhizophila (macrolides), Escherichia coli (quinolones), B. cereus (tetracyclines) and B. subtilis at pH 7.0 (sulphonamides), respectively. Twenty-three antimicrobial substances were analysed and a logistic regression was established for each substance assayed to relate the antibiotic concentration and the zone of microbial growth inhibition. Great linearity in the response was observed (regression coefficients of over 0.97). This fact suggests the possibility of establishing a decision level of antibiotic concentrations near to the Maximum Residue Limits (MRL). Zones of inhibition were suggested as proposed action levels for the different antimicrobial groups (diameters of inhibition of 18 mm for the aminoglycoside, beta-lactam and sulphonamide plates; 19 mm for the tetracycline plate, 21 mm for the macrolide plate, and 24 mm for the quinolone plate). Specificity and cross-reactivity were also assayed.
authors.affiliation: Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, R.P.L. Kreder 2805, 3080 Esperanza, Argentina.
authors.forename: Rafael
authors.initials: R
authors.lastname: Althaus
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Berruga
authors.forename: Ana
authors.initials: A
authors.lastname: Montero
authors.forename: Marta
authors.initials: M
authors.lastname: Roca
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Molina
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D005260:Female; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D000900:Anti-Bacterial Agents