title: Fingerprint analysis of anti-tumor active polypeptides from Arca subcrenata by HPLC.
abstract: RP-HPLC was applied to analyze active polypeptides in Arca subcrenata, and the optimal condition for separation was also set up: temperature: 30 degrees C; wavelength: 280 nm; flow rate: 1.0 ml/min; Solvent A consisted of 80% acetonitrile and 0.1% trifluoroacetic acid (TFA) and solvent B contained 0.1% TFA. In this condition, ten samples' fingerprints were gained, in one of which the genuine fraction exhibited fourteen "common peaks" representing the characteristics of the constituents.
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Sheng-fang
authors.initials: SF
authors.lastname: Ren
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Song
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Yan
authors.forename: Ting-fei
authors.initials: TF
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Rong-min
authors.initials: RM
authors.lastname: Yu
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D049872:Bivalvia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D000097:Acetonitriles; D000970:Antineoplastic Agents; D010455:Peptides; D012997:Solvents