Effect of CoQ homologues on reactive oxygen generation by mitochondria.
Effect of CoQ compounds (Qs) on reactive oxygen (ROS) generation by mitochondrial complex I was studied using rat liver mitochondria and chemiluminescence probe L012. Kinetic analysis revealed that short chain Qs, such as Q2 and idebenone enhanced ROS generation by mitochondrial NADH oxidase system by a succinate-inhibitable mechanism. Lipid peroxidation in mitochondrial membranes induced by NADH and iron was inhibited by short chain Qs. The inhibitory activity was enhanced by co-oxidation of succinate as determined by chemiluminescence method and by electron spin resonance spectroscopy. These results suggested that the reduced form of short chain Qs inhibited mitochondrial ROS generation and lipid peroxidation.
Imada
Isuke
I
Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Osaka, Japan. imada@med.osaka-cu.ac.jp
Sato
Eisuke F
EF
Kira
Yukimi
Y
Inoue
Masayasu
M
D000818:Animals; D008297:Male; D053858:Metabolic Networks and Pathways; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
D017382:Reactive Oxygen Species; D014451:Ubiquinone; D010088:Oxidoreductases; C022691:succinate oxidase; C024989:coenzyme Q10; C036619:idebenone; C030571:ubiquinone 9