Differentially expressed genes from the glioblastoma cell line SHG-44 treated with all-trans retinoic acid in vitro.
Morphology, immunocytochemistry, growth curve assay, and flow cytometry were used to investigate the effects of all-trans retinoic acid (RA) on cell proliferation, cell cycle progression and differentiation of the astrocytoma cell line SHG-44 from glioblastoma multiforme (World Health Organization grade IV). The differentially expressed genes from RA-treated and normal SHG-44 were identified by cDNA microarray after the cell line SHG-44 was treated with 10muM RA for 3 days. Validation of some differentially expressed genes was performed by Northern Blot analysis. The expression of glial fibrillary acidic protein (GFAP) was markedly increased in RA-treated SHG-44 cells. Other changes included a short shuttle shape, small nucleus, decreased karyoplasm proportion, the formation of increased thin cytoplasmic processes, reduced cell growth and a 15% increase in G0/G1 phase cell populations. In addition, 42 known genes were identified with altered expression in our cDNA microarray. There was stable down-regulation of MDM2 and UGB as well as overexpression of SOD2, CSTB, and G3BP when RA-treated SHG-44 was compared with normal SHG-44. RA simultaneously suppressed the proliferation of SHG-44 cells significantly as well as induced differentiation and altered gene expression.
Zeng
Yi
Y
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan Province, 610041, China.
Yang
Zhong
Z
Xu
Jian-Guo
JG
Yang
Meng-Su
MS
Zeng
Zhi-Xiong
ZX
You
Chao
C
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005909:Glioblastoma / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D005904:Glial Fibrillary Acidic Protein; D014212:Tretinoin