title: A rice kinase-protein interaction map.
abstract: Plants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function. We previously developed a cost-effective high-throughput yeast two-hybrid system. Using this system, we have generated a protein interaction map of 116 representative rice kinases and 254 of their interacting proteins. Overall, the resulting interaction map supports a large number of known or predicted kinase-protein interactions from both plants and animals and reveals many new functional insights. Notably, we found a potential widespread role for E3 ubiquitin ligases in pathogen defense signaling mediated by receptor-like kinases, particularly by the kinases that may have evolved from recently expanded kinase subfamilies in rice. We anticipate that the data provided here will serve as a foundation for targeted functional studies in rice and other plants. The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed.
authors.affiliation: Department of Plant Pathology , University of Florida, Gainesville, Florida 32611, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ding
authors.forename: Todd
authors.initials: T
authors.lastname: Richter
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Fujii
authors.forename: Young Su
authors.initials: YS
authors.lastname: Seo
authors.forename: Mingtang
authors.initials: M
authors.lastname: Xie
authors.forename: Xianwu
authors.initials: X
authors.lastname: Zheng
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Kanrar
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Stevenson
authors.forename: Christopher
authors.initials: C
authors.lastname: Dardick
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fahong
authors.initials: F
authors.lastname: Yu
authors.forename: Laura E
authors.initials: LE
authors.lastname: Bartley
authors.forename: Mawsheng
authors.initials: M
authors.lastname: Chern
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bart
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Chen
authors.forename: Lihuang
authors.initials: L
authors.lastname: Zhu
authors.forename: William G
authors.initials: WG
authors.lastname: Farmerie
authors.forename: Michael
authors.initials: M
authors.lastname: Gribskov
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.forename: Michael E
authors.initials: ME
authors.lastname: Fromm
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; D011494:Protein Kinases; D047390:Casein Kinase II; D018844:Cyclin-Dependent Kinases; D020928:Mitogen-Activated Protein Kinases