title: Paraoxonase-1 (PON1) activity as a risk factor for atherosclerosis in chronic renal failure patients.
abstract: Paraoxonase is a high-density lipoprotein-associated enzyme and has been shown to reduce the susceptibility to low-density lipoprotein peroxidation. This study aimed to investigate the activity of serum paraoxonase in uremic patients on hemodialysis (HD) and in the predialysis period, and to evaluate the correlations of vascular disease with paraoxonase activity. Thirty patients with chronic renal failure (CRF) undergoing HD (group 1), 30 patients with CRF under conservative treatment (group 2), and 30 healthy controls (group 3) were included. Basal, salt-stimulated, and arylesterase activity were tested by UV spectrophotometry. Serum lipid parameters were determined. B-Mode Doppler ultrasound was used to assess common carotid intima-media thickness (IMT). Basal paraoxonase, salt-stimulated, and arylesterase activity showed no significant difference between group 1 and group 2. However, it was significantly lower in group 1 and in group 2 than controls. Carotid IMT was significantly higher in group 1 than group 2 and both were significantly higher than controls. Basal paraoxonase-1 (PON1), salt-stimulated PON1, and arylesterase activity correlate with BUN, but only basal PON1 and salt-stimulated PON1 correlate with serum albumin. Linear regression showed that the most significant determinant of carotid IMT was PON1 arylesterase activity in group 1 and arylesterase activity and basal PON1 activity in group 2. Patients with CRF, whether under HD or conservative treatment, have reduced basal and stimulated paraoxonase activities, and this could be an important factor causing increased vascular disease in those patients. Modifying this factor can be of great value to protect against this common complication.
authors.affiliation: Departments of Nephrology, Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt. drsaeed33@gmail.com
authors.forename: Saeed Abdelwhab
authors.initials: SA
authors.lastname: Saeed
authors.forename: Magdy
authors.initials: M
authors.lastname: Elsharkawy
authors.forename: Kadry
authors.initials: K
authors.lastname: Elsaeed
authors.forename: Osman
authors.initials: O
authors.lastname: Fooda
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D001806:Blood Urea Nitrogen; D002097:C-Reactive Protein / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D002340:Carotid Artery Diseases / Q000000981:diagnostic imaging / Q000453:epidemiology* / Q000378:metabolism*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000453:epidemiology* / Q000378:metabolism* / Q000628:therapy; D016014:Linear Models; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000706:statistics & numerical data*; D012307:Risk Factors; D017539:Tunica Intima / Q000000981:diagnostic imaging; D017540:Tunica Media / Q000000981:diagnostic imaging; D014463:Ultrasonography; D014511:Uremia / Q000453:epidemiology / Q000378:metabolism / Q000628:therapy; D055815:Young Adult
chemical_list: D002097:C-Reactive Protein; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human