Growth inhibition and induction of apoptosis of colon cancer cell lines by applying marine phospholipid.
Polyunsaturated fatty acids (PUFAs) exhibit beneficial biological functions in carcinogenic processes. We examined the effects of PUFAs in the acid and phospholipid forms on three colon cancer cell lines (HT-29, Caco-2, and DLD-1). Docosahexaenoic acid (DHA) and eicosapentaenoic (EPA) in both acid and phospholipid forms showed growth inhibition effects on experimental colon cancer cell lines. But these PUFAs had the strongest growth-inhibitory effect on HT-29 than Caco-2 and DLD-1. Combined application of PUFAs and sodium butyrate (NaBt) increased the growth inhibition. Growth inhibition was apparently caused by increased lipid peroxidation. DHA or EPA in combination with NaBt significantly increased caspase-3 activity compared to control. DHA and DHA-rich phosphatidylcholine decreased Bcl-2 level in HT-29 and Caco-2 cells.
Hossain
Zakir
Z
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan. zakirh1000@yahoo.com
Hosokawa
Masashi
M
Takahashi
Koretaro
K
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D016718:Arachidonic Acid / Q000737:chemistry; D047271:Asterias / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002087:Butyrates / Q000378:metabolism / Q000494:pharmacology; D018938:Caco-2 Cells; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D049832:Decapodiformes / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000378:metabolism / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology*
D000975:Antioxidants; D002087:Butyrates; D016861:Cyclooxygenase Inhibitors; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D002084:Butylated Hydroxytoluene; D016718:Arachidonic Acid; D053148:Caspase 3; D007213:Indomethacin