title: A new artificial antigen of the hepatitis E virus.
abstract: An artificial antigen composed of 12 small antigenic regions derived from the ORF2 and ORF3 HEV proteins was designed. The gene encoding for this artificial antigen was assembled from synthetic oligonucleotides by a new method called Restriction Enzyme-Assisted Ligation (REAL). The diagnostic relevance of this second generation HEV mosaic protein (HEV MA-II) was demonstrated by testing this antigen against a panel of 142 well defined anti-HEV positive and anti-HEV negative serum samples. The data obtained in this study support the substantial diagnostic potential of this HEV mosaic antigen.
authors.affiliation: RPC Diagnostic Systems, Nizhniy, Novgorod, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Ulanova
authors.forename: A P
authors.initials: AP
authors.lastname: Obriadina
authors.forename: G
authors.initials: G
authors.lastname: Talekar
authors.forename: A N
authors.initials: AN
authors.lastname: Burkov
authors.forename: H A
authors.initials: HA
authors.lastname: Fields
authors.forename: Y E
authors.initials: YE
authors.lastname: Khudyakov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006508:Hepatitis Antibodies / Q000097:blood / Q000276:immunology; D018963:Hepatitis Antigens / Q000235:genetics / Q000276:immunology*; D016751:Hepatitis E / Q000175:diagnosis* / Q000276:immunology; D016752:Hepatitis E virus / Q000276:immunology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D006508:Hepatitis Antibodies; D018963:Hepatitis Antigens; D009841:Oligonucleotides; D011994:Recombinant Proteins; D014764:Viral Proteins