Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea.
Carbapenem-resistant Acinetobacter spp. are being increasingly reported worldwide, including in South Korea, where we examined 144 representative isolates collected in a nationwide hospital survey in 2005. Metallo-beta-lactamases were detected in only 19.4% of isolates, none of which were Acinetobacter baumannii, whereas 74.3% of isolates (mostly A. baumannii) expressed bla(OXA) carbapenemase genes. Among the latter, 47 had bla(OXA-23)-like genes and 56 had upregulated bla(OXA-51)-like variants, including bla(OXA-66), (-83), (-109) and (-115); bla(OXA-115) was a novel variant, detected in two isolates. bla(OXA-72) (bla(OXA-40)-like) was detected in only a single Acinetobacter baylyi isolate, whilst three Acinetobacter calcoaceticus isolates had both bla(VIM-2)-like and bla(OXA-58) genes. Pulsed-field gel electrophoresis (PFGE) suggested the spread of A. baumannii clones with OXA carbapenemases within and between hospitals. In conclusion, the recent increase in imipenem-resistant Acinetobacter spp. from South Korea is mostly due to OXA-type carbapenemases.
Lee
Kyungwon
K
Yonsei University College of Medicine, Seoul, South Korea.
Kim
Mi-Na
MN
Choi
Tae Yeal
TY
Cho
Soung-Eun
SE
Lee
Seungok
S
Whang
Dong Hee
DH
Yong
Dongeun
D
Chong
Yunsop
Y
Woodford
Neil
N
Livermore
David M
DM
D000150:Acinetobacter / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003428:Cross Infection / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007723:Korea; D001618:beta-Lactamases / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase