Detection of cytoplasmic and nuclear localization of adenomatous polyposis coli (APC) protein in cells.
The adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers. APC is a multi-domain protein whose distribution at different subcellular locations correlates with unique cellular processes. Our laboratory has focused on the link between APC subcellular location and function, and has characterized pathways for the trafficking of APC both into and out of the nucleus. Antibody specificity is an important factor in the determination of APC localization, and in this chapter we outline a strategy for the unambiguous detection of APC using a combination of biochemical and cell biology approaches.
Brocardo
Mariana
M
Westmead Millennium Institute, The University of Sydney, NSW, Australia.
Henderson
Beric R
BR
D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D034622:RNA Interference; D013347:Subcellular Fractions / Q000378:metabolism*
D025601:Adenomatous Polyposis Coli Protein