title: Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta).
abstract: The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been integrated into the genome of gametophytes of L. japonica, and the expression product showed the expected bioactivity, implying the proper post-transcript modification in haploid gametophyte.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
authors.forename: YiChen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: JiangTao
authors.initials: J
authors.lastname: Gao
authors.forename: JianMin
authors.initials: J
authors.lastname: Liao
authors.forename: ShiJing
authors.initials: S
authors.lastname: Sun
authors.forename: ZiLong
authors.initials: Z
authors.lastname: Shen
authors.forename: Song
authors.initials: S
authors.lastname: Qin
mesh_terms: D019149:Bioreactors; D005343:Fibrinolytic Agents / Q000378:metabolism*; D018014:Gene Transfer Techniques; D006801:Humans; D007795:Laminaria / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D008297:Male; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000235:genetics / Q000378:metabolism*
chemical_list: D005343:Fibrinolytic Agents; D011994:Recombinant Proteins; C087896:reteplase; D010959:Tissue Plasminogen Activator