Protective effect of daidzin against D-galactosamine and lipopolysaccharide-induced hepatic failure in mice.
This study examined the effects of daidzin, a major isoflavone from Puerariae Radix, on D-galactosamine (D-GalN) and lipopolysaccharide (LPS)-induced liver failure. Mice were given an intraperitoneal injection of daidzin (25, 50, 100 and 200 mg/kg) 1 h before receiving an injection of D-GalN (700 mg/kg)/LPS (10 microg/kg). Daidzin markedly reduced the elevated serum aminotransferase activity and the levels of lipid peroxidation and tumor necrosis factor-alpha. The glutathione content was lower in the D-GalN/LPS group, which was attenuated by daidzin. The daidzin pretreatment attenuated the swollen mitochondria observed in the d-GalN/LPS group. Daidzin attenuated the apoptosis of hepatocytes, which was confirmed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and a caspase-3 assay. Overall, these results suggest that the liver protection of daidzin is due to reduced oxidative stress and its antiapoptotic activity.
Kim
Sung-Hwa
SH
College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, Korea.
Heo
Jeong-Haing
JH
Kim
Yeong Shik
YS
Kang
Sam Sik
SS
Choi
Jae Sue
JS
Lee
Sun-Mee
SM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005688:Galactosamine / Q000009:adverse effects; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000009:adverse effects; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D017093:Liver Failure / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008933:Mitochondrial Swelling / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D029908:Pueraria / Q000737:chemistry; D000637:Transaminases / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C442842:plant extract, Puerariae Radix; C013908:daidzin; D005688:Galactosamine; D000637:Transaminases; D053148:Caspase 3; D005978:Glutathione