Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC.
The principal goal of this work was to develop an efficient method for the simultaneous determination of four pyrethroid (PYR) insecticides, cyfluthrin, cyhalothrin, cypermethrin, and deltamethrin, in porcine muscle and pasteurized milk using liquid-liquid extraction (LLE). Sample extraction was carried out with and without additional column cleanup procedures, and the final determination was made using GC with electron-capture detector (ECD). The pesticide identity was confirmed using GC-MS in the SIM mode. Since there were minor differences between the extraction procedures, extraction without the additional cleanup procedure was used throughout the work. The method was validated by fortifying blank samples with half, two, and four times the maximum residue limit (MRL) of each PYR. The average recoveries (n = 6) ranged from 83.5 to 99.2% and 82.9 to 109% in porcine muscle and pasteurized milk, respectively. The repeatability of measurements expressed as RSDs, was in the range of 1.7-11.9 and 1.5-10.3% in porcine muscle and pasteurized milk, respectively. The LODs ranged from 3.3 to 9 and 3 to 8.1 ppm, whereas the LOQs ranged from 10 to 27.4 and 9 to 24.6 ppm, in porcine muscle and pasteurized milk, respectively. The applicability of the method was demonstrated by analyzing real samples collected from major cities in the Republic of Korea. No residues of the selected pesticides were detected in any of the samples.
Khay
Sathya
S
Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of Korea.
Abd El-Aty
A M
AM
Choi
Jeong-Heui
JH
Shin
Eun-Ho
EH
Shin
Ho-Chul
HC
Kim
Jin-Suk
JS
Chang
Byung-Joon
BJ
Lee
Chi-Ho
CH
Shin
Sung-Chul
SC
Jeong
Ji Yoon
JY
Shim
Jae-Han
JH
D000818:Animals; D004583:Electrons; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006358:Hot Temperature; D008460:Meat / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry*; D011722:Pyrethrins / Q000032:analysis* / Q000737:chemistry*; D013552:Swine
D010573:Pesticide Residues; D011722:Pyrethrins