title: Study on an on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography for separation and determination of trace estrone in environment.
abstract: Estrone is one of the important potential endocrine-disrupting compounds, and the sensitive and reliable analytical methods for the determination of estrone are required for the assurance of human health. In this paper, using estrone as template molecule, 3-aminopropyltriethoxysilane as function monomer, and tetraethoxysilicane as cross-linker, a highly selective molecularly imprinted microsphere was synthesized by surface molecular imprinting technique combined with a sol-gel process. The imprinted material was characterized by the Fourier transform infrared and static adsorption experiments, and the results showed that it exhibited good recognition and selective ability for estrone. A novel method for separation and determination of trace estrone in environmental sample was developed using on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography. With a sample loading flow rate of 2.6 mL min(-1) for a 9.6-min extraction, the enrichment factor obtained by the slopes of the linear portion in comparison with the direct injection of 10 microL standard sample solution was 1,045. The detection limit (S/N = 3) was 5.7 ng L(-1), and the relative standard deviations for nine replicate extractions of 5.0 microg L(-1) estrone was less than 10.0%. This method was evaluated for quantitative determination of estrone in well and lake water samples spiked at two levels (0.5 and 1.0 microg L(-1)) with recoveries ranging from 86% to 95%.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Guozhen
authors.initials: G
authors.lastname: Fang
authors.forename: Hua
authors.initials: H
authors.lastname: Pingzhu
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D004970:Estrone / Q000032:analysis*; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004785:Environmental Pollutants; D011108:Polymers; D004970:Estrone