title: Liquid chromatography-mass spectrometry analysis of macranthoidin B, macranthoidin A, dipsacoside B, and macranthoside B in rat plasma for the pharmacokinetic investigation.
abstract: A liquid chromatography-electrospray ionization-mass spectrometry method has been developed and validated for identification and quantification of four major bioactive saponins in rat plasma after oral administration of extraction of saponins from Flos Lonicerae, i.e., macranthoidin B, macranthoidin A, dipsacoside B, and macranthoside B. Plasma samples were extracted with solid-phase extraction, separated on a Shim-pack CLC-ODS column and detected by MS in negative selective ion monitoring mode. Calibration curves offered linear ranges of two orders of magnitude with r(2)>0.999. The method showed the low limit quantification of 7.72, 6.06, 7.16, and 1.43 ng/mL for macranthoidin B, macranthoidin A, dipsacoside B, and macranthoside B, respectively. The inter- and intra-CV precision (R.S.D.) were all within 10% and accuracy (% bias) ranged from -10 to 10%. The overall recovery was more than 70%. This developed method was subsequently successfully applied to pharmacokinetic profiles of the four saponins in rats. After oral administration of extraction of saponins in rats, the concentration-time course was found to be the double peaks of curve.
authors.affiliation: Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
authors.forename: Chun-Yun
authors.initials: CY
authors.lastname: Chen
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.forename: Ling
authors.initials: L
authors.lastname: Yi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000493:pharmacokinetics; D016014:Linear Models; D031239:Lonicera / Q000737:chemistry; D013058:Mass Spectrometry; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; C083463:macranthoidin B; C083462:macranthoidin A; C083461:dipsacoside B; D009828:Oleanolic Acid