title: Study of the effect of sample preparation and cooking on the selenium speciation of selenized potatoes by HPLC with ICP-MS and electrospray ionization MS/MS.
abstract: The efficiency of enzymatic hydrolysis and leaching with water using accelerated solvent extraction (ASE) or boiling was investigated for quantitative Se speciation in selenized potatoes using reversed phase HPLC coupled to ICP-MS. Preliminary identification of selenomethionine (SeMet), Se-methylselenocysteine (SeMeCys), and selenate in extracts of potato skin and flesh was achieved using complementary reversed phase and anion-exchange HPLC-ICP-MS and retention time matching with standards. The quantitative speciation data revealed a higher percentage of selenomethionine (73% of the total Se) found in the flesh in comparison with skin (containing 21% of the total Se as SeMet). ASE and boiling in water were found to be similar in terms of Se extraction efficiency and profiles. However, ASE was found to be more efficient than boiling with respect to sample cleanup and reduced sample handling. The presence of SeMet at parts per billion levels in selenized potatoes was confirmed by reversed phase HPLC with online ESI MS/MS.
authors.affiliation: LGC Limited, Queens Road, Teddington, Middlesex, United Kingdom. Heidi.Goenaga-Infante@lgc.co.uk
authors.forename: Heidi Goenaga
authors.initials: HG
authors.lastname: Infante
authors.forename: Ana Arias
authors.initials: AA
authors.lastname: Borrego
authors.forename: Emma
authors.initials: E
authors.lastname: Peachey
authors.forename: Ruth
authors.initials: R
authors.lastname: Hearn
authors.forename: Gavin
authors.initials: G
authors.lastname: O'Connor
authors.forename: Tamara García
authors.initials: TG
authors.lastname: Barrera
authors.forename: José Luis Gómez
authors.initials: JL
authors.lastname: Ariza
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005527:Food, Fortified / Q000032:analysis; D006358:Hot Temperature; D011492:Protein Hydrolysates; D012643:Selenium / Q000008:administration & dosage* / Q000032:analysis; D018036:Selenium Compounds / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D011492:Protein Hydrolysates; D018036:Selenium Compounds; C007978:hydrolysin; D012643:Selenium