Aberrant allele-specific replication, independent of parental origin, in blood cells of cancer patients.
BACKGROUND
Allelic counterparts of biallelically expressed genes display an epigenetic symmetry normally manifested by synchronous replication, different from genes subjected to monoallelic expression, which normally are characterized by an asynchronous mode of replication (well exemplified by the SNRPN imprinted locus). Malignancy was documented to be associated with gross modifications in the inherent replication-timing coordination between allelic counterparts of imprinted genes as well as of biallelically expressed loci. The cancer-related allelic replication timing aberrations are non-disease specific and appear in peripheral blood cells of cancer patients, including those with solid tumors. As such they offer potential blood markers for non-invasive cancer test. The present study was aimed to gain some insight into the mechanism leading to the replication timing alterations of genes in blood lymphocytes of cancer patients.


METHODS
Peripheral blood samples derived from patients with prostate cancer were chosen to represent the cancerous status, and samples taken from patients with no cancer but with benign prostate hyperplasia were used to portray the normal status. Fluorescence In Situ Hybridization (FISH) replication assay, applied to phytohemagglutinin (PHA)-stimulated blood lymphocytes, was used to evaluate the temporal order (either synchronous or asynchronous) of genes in the patients' cells.


RESULTS
We demonstrated that: (i) the aberrant epigenetic profile, as delineated by the cancer status, is a reversible modification, evidenced by our ability to restore the normal patterns of replication in three unrelated loci (CEN15, SNRPN and RB1) by introducing an archetypical demethylating agent, 5-azacytidine; (ii) following the rehabilitating effect of demethylation, an imprinted gene (SNRPN) retains its original parental imprint; and (iii) the choice of an allele between early or late replication in the aberrant asynchronous replication, delineated by the cancer status, is not random but is independent of the parental origin.


CONCLUSIONS
The non-disease specific aberrant epigenetic profile displayed in peripheral blood cells of patients with a solid tumour (unlike genetic aberrations) can be reversed, by an epigenetic drug applied in vitro, to the normal. It appears that the cancerous status differentiates between two allelic counterparts in a non-random manner, but independent of the parental origin.
Dotan
Zohar A
ZA
Department of Urology, Sheba Medical Center, Tel-Hashomer 52621, Israel. Zohar.Dotan@sheba.health.gov.il
Dotan
Aviva
A
Ramon
Jacob
J
Avivi
Lydia
L
D000483:Alleles; D001374:Azacitidine / Q000008:administration & dosage; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D018392:Genomic Imprinting / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000235:genetics; D055517:snRNP Core Proteins / Q000235:genetics
D016160:Retinoblastoma Protein; C526417:SNRPN protein, human; D055517:snRNP Core Proteins; D001374:Azacitidine