Image analysis of calcium release dynamics.
Many aspects of animal development are dependent on the dynamic release of calcium (Ca2+) ions. Although Ca2+ release within a cell is tightly controlled, how the release dynamics result in a specific biological output in embryonic development is less clear. The integration of pharmacological and molecular-genetic studies with in vivo imaging in zebrafish and Xenopus has linked endogenous Ca2+ release to the Wnt signaling network. Our data suggests that distinct Ca2+ release dynamics lead to antagonism of the developmentally important Wnt/beta-catenin pathway while sustained Ca2+ release modulates polarized cell movements.
Freisinger
Christina M
CM
Department of Biology, University of Iowa, Iowa City, USA.
Houston
Douglas W
DW
Slusarski
Diane C
DC
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008845:Microinjections; D051153:Wnt Proteins / Q000378:metabolism; D015027:Zebrafish
D051153:Wnt Proteins; D002118:Calcium