Hemolytic activity of Trichomonas gallinae isolates does not correspond with clinical virulence.
The hemolytic activity of 22 Trichomonas gallinae isolates was investigated using an 18h erythrocyte hemolysis assay which has been shown to correlate with the clinical virulence of T. vaginalis. Absorbance of the assay supernatants was measured at 540nm and expressed as percentage of complete hemolysis. Mean hemolytic activity of the T. gallinae isolates ranged from 3.5% to 53.4% and did not correspond with clinical virulence. The results of this investigation suggest hemolytic activity is not a useful in vitro virulence assay for T. gallinae.
Gerhold
Richard W
RW
College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA. rgerhold@uga.edu
Yabsley
Michael J
MJ
Fischer
John R
JR
D000818:Animals; D001715:Bird Diseases / Q000469:parasitology / Q000473:pathology; D001717:Birds; D002645:Chickens / Q000097:blood; D046508:Culture Techniques / Q000662:veterinary*; D004912:Erythrocytes / Q000469:parasitology*; D006460:Hemolysin Proteins / Q000032:analysis*; D006461:Hemolysis; D011201:Poultry Diseases / Q000469:parasitology / Q000473:pathology; D014244:Trichomonas / Q000472:pathogenicity*; D014245:Trichomonas Infections / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D014774:Virulence
D006460:Hemolysin Proteins