title: [AcSDKP attenuates PDGF-induced cardiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway].
abstract: OBJECTIVE
  To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2 (ERK1/2) pathway on this process.
  
  
  METHODS
  Metabolic activity of fibroblasts was determined by CCK-8. Cell cycle was detected by flow cytometry. Expressions of type I and type III collagen were measured by immunocytochemistry and Western blot. Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot.
  
  
  RESULTS
  10(-9) mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2, while the protein levels of ERK1/2 were not significantly affected by AcSDKP.
  
  
  CONCLUSIONS
  AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.
authors.affiliation: Experimental Center, North China Coal Medical University, Tangshan 063000, China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Wen-dong
authors.initials: WD
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Rui-min
authors.initials: RM
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Jing-bo
authors.initials: JB
authors.lastname: Yan
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
chemical_list: D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D003094:Collagen; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C058504:goralatide