Effect of a standardised dietary restriction protocol on multiple laboratory strains of Drosophila melanogaster.
BACKGROUND
Outcomes of lifespan studies in model organisms are particularly susceptible to variations in technical procedures. This is especially true of dietary restriction, which is implemented in many different ways among laboratories.


RESULTS
In this study, we have examined the effect of laboratory stock maintenance, genotype differences and microbial infection on the ability of dietary restriction (DR) to extend life in the fruit fly Drosophila melanogaster. None of these factors block the DR effect.


CONCLUSIONS
These data lend support to the idea that nutrient restriction genuinely extends lifespan in flies, and that any mechanistic discoveries made with this model are of potential relevance to the determinants of lifespan in other organisms.
Grandison
Richard C
RC
UCL Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
Wong
Richard
R
Bass
Timothy M
TM
Partridge
Linda
L
Piper
Matthew D W
MD
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D031204:Caloric Restriction; D004032:Diet; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D004435:Eating; D005838:Genotype; D008017:Life Expectancy; D023421:Models, Animal; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D013752:Tetracycline