title: cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1.
abstract: The liver cDNA encoding heme oxygenase--1 (HO-1) was sequenced from European sea bass (Dicentrarchus labrax) (accession number no. EF139130). The HO-1 cDNA was 1250 bp in nucleotide length and the open reading frame encoded 277 amino acid residues. The deduced amino acid sequence of the European sea bass had 75% and 50% identity with the amino acid sequences of tetraodontiformes (Tetraodon nigroviridis and Takifugu rubripes) and human HO-1 proteins, respectively. A short hydrophobic transmembrane domain at the C--terminal region was found, and four histidine residues were highly conserved, including human his25 that is essential for HO catalytic activity. RT-PCR of mRNA from eight different European sea bass tissues revealed that, in a homeostatis state, the heme oxygenase--1 was abundant in the spleen and liver but not in the brain.
authors.affiliation: Laboratoire PROTEE Toulon, Equipe de Biologie Moléculaire Marine, Université du Sud équipe de Biologie Moléculaire Marine-Var, France.
authors.forename: N
authors.initials: N
authors.lastname: Prevot-D'Alvise
authors.forename: S
authors.initials: S
authors.lastname: Pierre
authors.forename: S
authors.initials: S
authors.lastname: Gaillard
authors.forename: E
authors.initials: E
authors.lastname: Gouze
authors.forename: J-N
authors.initials: JN
authors.lastname: Gouze
authors.forename: J
authors.initials: J
authors.lastname: Aubert
authors.forename: S
authors.initials: S
authors.lastname: Richard
authors.forename: J-P
authors.initials: JP
authors.lastname: Grillasca
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D005602:France; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000737:chemistry / Q000235:genetics*; D006639:Histidine / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D006639:Histidine; D051547:Heme Oxygenase-1