title: [Construction of tissue-engineering skin with carrier of active composite dermal matrix].
abstract: OBJECTIVE
  To construct of tissue engineering skin including active composite dermal matrix.
  
  
  METHODS
  The human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.
  
  
  RESULTS
  The structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.
  
  
  CONCLUSIONS
  Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.
authors.affiliation: Burns Institute, the First Affiliated Hospital to the PLA General Hospital, Beijing 100037, PR China.
authors.forename: Zhong-feng
authors.initials: ZF
authors.lastname: Ma
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Hong-ming
authors.initials: HM
authors.lastname: Yang
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Liang
authors.forename: Ming-huo
authors.initials: MH
authors.lastname: Xu
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D024042:Collagen Type I; D020405:Dermis / Q000637:transplantation*; D000078404:Epidermal Cells; D005109:Extracellular Matrix / Q000637:transplantation*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D012867:Skin / Q000166:cytology; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I