title: Frizzled-7-dependent tissue separation in the Xenopus gastrula.
abstract: Formation of tissue boundaries can be studied in a simple, inexpensive system, the Xenopus gastrula. Here, the internalized mesoderm and endoderm are separated from the ectodermal blastocoel roof by Brachet's cleft. Non-canonical Wnt signaling mediated by the Wnt receptor, Xfz-7, is essential for this tissue separation event. The function of Wnt pathway components and other factors in tissue separation at Brachet's cleft can be tested in a blastocoel roof assay. Small pieces of mesoderm or endoderm are placed on large blastocoel roof explants, and it is observed whether these test explants remain on the surface of their in vivo substratum, or sink into it.
authors.affiliation: Dept of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Winklbauer
authors.forename: Olivia
authors.initials: O
authors.lastname: Luu
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D005775:Gastrula / Q000033:anatomy & histology / Q000502:physiology; D009024:Morphogenesis; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology*
chemical_list: C492040:Fzd7 protein, Xenopus; D043562:Receptors, G-Protein-Coupled; D029867:Xenopus Proteins