[Effect of hepatitis B virus C protein on function of natural killer cell in NK-92 cells].
OBJECTIVE
To investigate the influence of hepatitis B virus C protein on the function of natural killer cell.


METHODS
Recombinant eukaryotic expression plasmid pHBI-CMV-HBC was constructed and confirmed by double restrictive enzyme digestion and DNA sequencing analysis. Then the recombinant plasmid was transfected into NK-92 cells with lipofectamine encapsuled. The transfected NK-92 cells containing expressive HBV C protein was confirmed by Western Blot analysis. ELISA was employed to determine the IFN-gamma level secreted by NK-92 cells. And finally the cytotoxicities of NK cells were analysed by MTT colorimetry, with the hepatoblastoma cell line (HepG2) as target cell.


RESULTS
Western blotting confirmed the expression of HBV C protein in the NK-92 cells transfected with pHBI-CMV-HBC. NK cytotoxicities and IFN-gamma secretion level of NK-92 cells transfected with recombinant plasmid significantly increased compared to control NK-92 cells transfected with blank plasmid (P < 0.01) and untransfected NK-92 cells(P < 0.01).


CONCLUSIONS
Transient expression of HBC can increase IFN-gamma secretion and cytotoxicities of NK-92 cells.
Liu
Ying-Xia
YX
Shenzhen Liver Disease Research Institute, the Third Hospital Affiliated to Guangdong Medical College, Shenzhen 518020, China.
Wei
Xing-Hui
XH
Li
Bing
B
Luo
Zhi-Xiong
ZX
Xie
Jing-Jing
JJ
Tan
Yan
Y
Long
Su-Jun
SJ
Zhou
Bo-Ping
BP
D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D006509:Hepatitis B / Q000276:immunology* / Q000821:virology; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*
D006512:Hepatitis B Core Antigens; D007371:Interferon-gamma