title: High-potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa azurins.
abstract: Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu(II/I) reduction potentials relative to the Cu(II) aquo ion. The high potential and low axial hyperfine splitting (Cu(II) electron paramagnetic resonance A( parallel)) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.
authors.affiliation: Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: Kyle M
authors.initials: KM
authors.lastname: Lancaster
authors.forename: Keiko
authors.initials: K
authors.lastname: Yokoyama
authors.forename: John H
authors.initials: JH
authors.lastname: Richards
authors.forename: Jay R
authors.initials: JR
authors.lastname: Winkler
authors.forename: Harry B
authors.initials: HB
authors.lastname: Gray
mesh_terms: D001400:Azurin / Q000235:genetics* / Q000378:metabolism; D003300:Copper; D004578:Electron Spin Resonance Spectroscopy; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D015202:Protein Engineering; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D001400:Azurin; D003300:Copper