title: Anti-allergic activity of an ethanol extract from Salviae miltiorrhiza.
abstract: As part of an ongoing investigation aimed at the discovery of novel bioactive medicinal herbs with anti-inflammatory properties, the effects of an ethanolic extract from the parts of Salviae miltiorrhiza Bunge (ESM) were evaluated using in vitro and in vivo animal model analysis. ESM inhibited cyclooxygenase-2 (COX-2) and COX-1-dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with IC50 values of 3.96 microg/mL and 21.54 microg/mL, respectively. Furthermore, ESM inhibited leukotriene C4 (LTC4) production with an IC50 value of 2.6 microg/mL. These results clearly demonstrated the dual COX-2 selective/5-lipoxygenase inhibitory activity that ESM possessed. ESM strongly inhibited a degranulation reaction in a dose dependent manner within a BMMC system, with an IC50 value of 22.4 microg/mL. Additionally, ESM was tested in a rat passive cutaneous anaphylaxis (PCA) reaction assay by oral administration (25 to 100 mg/kg). ESM dose-dependently inhibited the PCA reaction, which was activated by anti-dinirophenyl (DNP) IgE. These results suggested that ESM might be beneficial in regulating various allergic reactions.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Korea.
authors.forename: Ju Hye
authors.initials: JH
authors.lastname: Yang
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D015550:Cell Degranulation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D000431:Ethanol; D007073:Immunoglobulin E / Q000276:immunology; D017997:Leukotriene C4 / Q000032:analysis / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D015230:Prostaglandin D2 / Q000096:biosynthesis / Q000235:genetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012997:Solvents
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012997:Solvents; D016718:Arachidonic Acid; D017997:Leukotriene C4; D007073:Immunoglobulin E; D000431:Ethanol; D011451:Prostaglandin-Endoperoxide Synthases; D015230:Prostaglandin D2