Behavior of endothelial cells regulated by a dynamically changed microenvironment of biodegradable PLLA-PC.
The influence of PLLA-PC membranes on the behavior of vascular ECs is studied. It was found that the membranes were not cytotoxic. The cell adhesion and spread on PLLA-PC 30/1 were initially depressed. After 1 d, however, cells attached and spread on the copolymer, and proliferated beyond 2 d. To elucidate the mechanism of this behavior, the surface morphology and the degradation products of the PLLA/PC 30/1 membrane during the culture period were tested, and Fg adsorption to the membranes was examined. It is concluded that the behavior of ECs can be regulated by a dynamically changed microenvironment of the PLLA-PC membrane. PLLA-PC copolymers might therefore serve as a novel material for tissue engineering.
Chen
Yuanwei
Y
State Key Laboratory of Polymer Materials and Engineering of China, College of Polymer Science & Engineering, Sichuan University, Chengdu, China.
Chen
Niancao
N
Qiu
Zhi
Z
Wang
Lijian
L
Wan
Changxiu
C
Luo
Xianglin
X
Li
Suming
S
D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004777:Environment; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D015394:Molecular Structure; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005340:Fibrinogen