Tackling multiple antibiotic resistance in enteropathogenic Escherichia coli (EPEC) clinical isolates: a diarylheptanoid from Alpinia officinarum shows promising antibacterial and immunomodulatory activity against EPEC and its lipopolysaccharide-induced inflammation.
Antibiotic treatment for infectious diseases commonly leads to host inflammatory responses. Molecules with bifunctional antibacterial and anti-inflammatory properties could provide a solution for such clinical manifestations. Here we report such bifunctional activity for a diarylheptanoid (5-hydroxy-7-(4''-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone) isolated from Alpinia officinarum, a medicinal plant belonging to the Zingiberaceae family, against enteropathogenic Escherichia coli (EPEC). The diarylheptanoid showed inhibitory and bactericidal activity against EPEC clinical isolates and efficiently suppressed EPEC lipopolysaccharide-induced inflammation in human peripheral blood mononuclear cells. In silico docking analysis revealed that the diarylheptanoid could interact with subunit A of E. coli DNA gyrase. Such molecules with bifunctional activity may be potential therapeutics for infectious diseases.
Subramanian
Krishnan
K
Centre for Biotechnology, Anna University, Chennai, India.
Selvakkumar
Chinnasamy
C
Vinaykumar
Kontham Sanathkumar
KS
Goswami
Nabajyoti
N
Meenakshisundaram
Sankaranarayanan
S
Balakrishnan
Arun
A
Lakshmi
Baddireddi Subhadra
BS
D032429:Alpinia / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019295:Computational Biology; D027081:DNA Gyrase / Q000378:metabolism; D036381:Diarylheptanoids / Q000302:isolation & purification / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects* / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008958:Models, Molecular; D011485:Protein Binding
D000900:Anti-Bacterial Agents; D036381:Diarylheptanoids; D029968:Escherichia coli Proteins; D007155:Immunologic Factors; D027081:DNA Gyrase