Modification and clearance of low density lipoproteins during the formation of endotoxin-lipoprotein complexes.
Changes in electrical charge and clearance rate of LDL after the formation of their complexes with bacterial LPS were studied in experiments on Wistar rats. It was found that binding of S. minnesota R595 LPS with (125)I-LDL sharply accelerated clearance of the greater part of LDL complexes, but on the other hand induced the appearance of an LDL-LPS subfraction with slower elimination rate compared to free LDL. Electrophoresis showed that after binding of LPS, LDL acquired a negative charge. These data suggest that the formation of LDL-LPS complexes is accompanied by modification of LDL due to which they acquire atherogenic properties.
Schvartz
Ya Sh
YSh
Institute of Therapy, Siberian Division of Russian Academy of Medical Sciences; Institute of Biochemistry, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk.
Polyakov
L M
LM
Dushkin
M I
MI
Pivovarova
E N
EN
D000818:Animals; D004594:Electrophysiology; D004731:Endotoxins / Q000097:blood / Q000378:metabolism*; D006207:Half-Life; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008070:Lipopolysaccharides / Q000097:blood / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008657:Metabolic Clearance Rate; D046912:Multiprotein Complexes / Q000097:blood / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D004731:Endotoxins; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D046912:Multiprotein Complexes