title: Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat.
abstract: Paraoxonases (PONs) are a small family of antioxidant enzymes whose antiatherogenic activity is well known. The aim of the present study was the evaluation of the effects of moderate aerobic training on their expression using a rat model. In order to discriminate between PON1 and PON3 enzymatic activity, we took advantage of some differences in their substrate preferences. PON1 and PON3 enzymatic activities and their protein levels were analyzed in plasma and in liver microsomes, and their mRNA levels in the liver. Exercise training did not affect PON1 expression or enzymatic activity but increased PON3 mRNA, protein levels, and enzymatic activity. Training also induced variations in plasma membrane composition, including an increase in polyunsaturated and a decrease in mono- and di-unsaturated fatty acids. On the other hand, acute exercise inhibited PON activities while increasing PON3 protein content in liver microsomes and reversing the relative composition in mono-, di-, and poly-unsaturated fatty acids, suggesting that physical stress, by altering membrane composition, may impair PON release from liver membranes. In conclusion, we documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training.
authors.affiliation: Department of Experimental Medicine, University of Perugia, Via del Giochetto, Perugia, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Romani
authors.forename: Gianna Evelina
authors.initials: GE
authors.lastname: De Medio
authors.forename: Simona
authors.initials: S
authors.lastname: di Tullio
authors.forename: Rosa
authors.initials: R
authors.lastname: Lapalombella
authors.forename: Irene
authors.initials: I
authors.lastname: Pirisinu
authors.forename: Vittoria
authors.initials: V
authors.lastname: Margonato
authors.forename: Arsenio
authors.initials: A
authors.lastname: Veicsteinas
authors.forename: Marina
authors.initials: M
authors.lastname: Marini
authors.forename: Gabriella
authors.initials: G
authors.lastname: Rosi
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009043:Motor Activity / Q000235:genetics / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010743:Phospholipids; D043303:Aryldialkylphosphatase