Effect of incorporation of alkyl linkers into siRNAs on RNA interference.
Unintended (off-target) transcript silencing is a critical problem associated with RNA interference (RNAi)-based therapeutic applications. This paper shows that the incorporation of appropriate alkyl linkers at the center of the sense strands can suppress the off-target effects induced by the sense strands without reducing the RNAi-inducing activity of the antisense strands.
Ueno
Yoshihito
Y
Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. uenoy@gifu-u.ac.jp
Yoshikawa
Kayo
K
Kitamura
Yoshiaki
Y
Kitade
Yukio
Y
D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002942:Circular Dichroism; D015195:Drug Design; D020869:Gene Expression Profiling; D020868:Gene Silencing; D023281:Genomics; D008156:Luciferases; D008956:Models, Chemical; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D048790:Renilla; D013696:Temperature
D016376:Oligonucleotides, Antisense; D034741:RNA, Small Interfering; D008156:Luciferases