title: Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection.
abstract: An analytical method for kanamycin A, amikacin and tobramycin of aminoglycoside (AG) antibiotics in milk samples was proposed using capillary zone electrophoresis (CZE) with post-column derivatization and laser-induced fluorescence detection. A simple and convenient homemade coaxial-gap reactor was adopted in the post-column derivatization of the AGs with 1.0 mmol/L naphthalene-2,3-dicarboxaldehyde and 8.0 mmol/L 2-mercaptoethanol in 35 mmol/L sodium tetraborate buffer (pH 10.0) of 30% (v/v) methanol. 50 mmol/L sodium acetate (pH 5.0) containing 0.5 mmol/L cetyltrimethyl ammonium bromide was used as the separation buffer. The linear calibration concentrations and detection limits were from 2.1 x 10(-5) to 5.0 x 10(-2)g/L and in the range of 7 x 10(-6) to 2 x 10(-5)g/L, respectively. The recoveries of the AGs in milk samples were from 81.6 to 93.1% (n=3).
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Chang-Zhu
authors.initials: CZ
authors.lastname: Yu
authors.forename: You-Zhao
authors.initials: YZ
authors.lastname: He
authors.forename: Guo-Ni
authors.initials: GN
authors.lastname: Fu
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Xie
authors.forename: Wu-Er
authors.initials: WE
authors.lastname: Gan
mesh_terms: D000583:Amikacin / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007612:Kanamycin / Q000032:analysis*; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014031:Tobramycin / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D007612:Kanamycin; D000583:Amikacin; D014031:Tobramycin