[Association between ADRA1A gene polymorphism and autoantibodies against the alpha1-adrenergic receptor in hypertensive patients.].
OBJECTIVE
To observe the association between ADRA1A gene polymorphism and autoantibodies against the alpha1-adrenergic receptor in hypertensive patients.


METHODS
A total of 396 patients with essential hypertension admitted to our hospital were selected and autoantibodies in sera were detected by ELISA, and patients were divided into the autoantibody positive and negative group. Genomic DNA was extracted from erythrocytes obtained from EDTA-treated blood by the Blood DNA extraction kit. Gene polymorphisms were detected by ligase detection reaction (LDR), including rs574584, rs1048101, rs3739216 and rs3802241. The frequency of genotypes and haplotype were analyzed.


RESULTS
The frequencies of detected genotypes between the autoantibody against the alpha1-adrenergic receptor positive group and negative group were similar (P > 0.05) while significant difference was in the frequencies of haplotypes (all P < 0.05). The frequencies of genotypes with rs1048101 (genotype C/C, C/T, P = 0.017) and rs3802241 (genotype A/A, A/G, P = 0.004) were significant different in autoantibody positive group compared to negative group in patients with stage 2.


CONCLUSIONS
ADRA1A gene polymorphism might correlate with the alpha1-adrenergic receptor autoantibody production in hypertensive patients.
Sun
Yan-Xiang
YX
Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science Technology, Wuhan 430022, China.
Liao
Yu-Hua
YH
Zhu
Feng
F
Wang
Min
M
Chen
Xiao
X
Chen
Fen
F
Cao
Ai-Lin
AL
Wang
Jun
J
D001323:Autoantibodies / Q000097:blood; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D011110:Polymorphism, Genetic; D018340:Receptors, Adrenergic, alpha-1
D001323:Autoantibodies; D018340:Receptors, Adrenergic, alpha-1