The source matters: no impact of the CCL2/MCP-1-1-2518G polymorphism of the donor on renal allograft outcome during the first year after transplantation.
Chemokines are involved in the recruitment of inflammatory cells to vascularized allografts. The chemokine CCL2/MCP-1 is expressed during allograft dysfunction, which is associated with the recruitment of inflammatory cells. Both intrinsic renal cells (donor origin) as well as infiltrating inflammatory cells (recipient origin) can be a source of CCL2/MCP-1. We previously demonstrated that the recipient MCP-1-2518G polymorphism is associated with increased CCL2/MCP-1 production by inflammatory cells and decreased renal allograft survival. We evaluated the impact of the MCP-1-2518G polymorphism in donor cells on renal allograft outcomes. We enrolled 252 recipients of kidney allografts in this retrospective study who had received grafts from 152 cadaveric donors. The CCL2/MCP-1 genotype was assessed using genomic DNA isolated from cryopreserved donor splenocytes. Outcome parameters studied were acute biopsy proven rejection (Banff criteria), serum creatinine, and glomerular filtration rate (GFR) at 1 year after transplantation, allograft loss, and death. MCP-1-2518 genotypes were in HW equilibrium. A/A was present in 125 (49.6%), A/G in 107 (42.5%), and G/G in 20 (7.9%) donor kidneys. There were no significant differences in the number of rejection episodes, the number of allograft losses, serum creatinine, GFR, or overall survival 1 year after transplantation. In contrast with the detrimental effect of the CCL2/MCP-1 polymorphism of the recipient, the CCL2/MCP-1 polymorphism of the donor has no impact on the allograft outcome during the first year after transplantation. The impact on the long-term outcomes needs further evaluation.
Lehmann
I
I
Medizinische Poliklinik, Campus Innenstadt, University of Munich, Germany.
Fischereder
M
M
Böhmig
G A
GA
Regele
H
H
Exner
M
M
Raith
M
M
Weiss
N
N
Segerer
S
S
D018932:Chemokine CCL2 / Q000235:genetics*; D018925:Chemokines / Q000235:genetics; D017931:DNA Primers; D005500:Follow-Up Studies; D005919:Glomerular Filtration Rate; D006801:Humans; D016030:Kidney Transplantation / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D015183:Restriction Mapping; D012189:Retrospective Studies; D014184:Transplantation, Homologous; D016896:Treatment Outcome
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D018925:Chemokines; D017931:DNA Primers