title: Pharyngeal Neisseria gonorrhoeae detection in oral-throat wash specimens of male patients with urethritis.
abstract: Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in the pharynx has been highlighted in the prevention of the unexpected spread of sexually transmitted diseases. We tried to clarify the detection rate of Neisseria gonorrhoeae in the pharynx and the clinical relevance of oral-throat wash specimens to detect the organism in heterosexual men with gonococcal and nongonococcal urethritis. In our cohort of 79 male patients with urethritis, oral throat wash specimens were collected after they had gargled with normal saline for approximately 30 to 60 s. Positive pharyngeal N. gonorrhoeae was defined as a positive result on the strand displacement amplification test for the specimen from the oral-throat wash. N. gonorrhoeae was detected in the oral-throat wash specimens of 13 (31.7%) of the 41 male patients with gonococcal urethritis. Oral-throat wash with a nucleic acid amplification test can detect pharyngeal N. gonorrhoeae easily and efficiently.
authors.affiliation: Department of Urology, Sapporo Medical University School of Medicine, and Sanjukai Hospital, S. 1, W. 16, Chuo-ku, Sapporo, 0608543, Japan. stakahas@sapmed.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kurimura
authors.forename: Jiro
authors.initials: J
authors.lastname: Hashimoto
authors.forename: Koh
authors.initials: K
authors.lastname: Takeyama
authors.forename: Mikio
authors.initials: M
authors.lastname: Koroku
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tanda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishimura
authors.forename: Taiji
authors.initials: T
authors.lastname: Tsukamoto
mesh_terms: D000293:Adolescent; D000328:Adult; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010608:Pharyngeal Diseases / Q000175:diagnosis* / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods; D014526:Urethritis / Q000382:microbiology*; D055815:Young Adult