title: Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio).
abstract: The mechanical properties of the axial muscles vary along the length of a fish's body. This variation in performance correlates with the expression of certain muscle proteins. Parvalbumin (PARV) is an important calcium binding protein that helps modulate intracellular calcium levels which set the size and shape of the muscle calcium transient. It therefore has a central role in determining the functional properties of the muscle. Transcript data revealed eight specific isoforms of PARV in common carp (Cyprinus carpio) skeletal muscle which we classified as alpha1 and beta1-7. This study is the first to show expression of all eight skeletal muscle PARV isoforms in carp at the protein level and relate regional differences in expression to performance. All of the PARV isoforms were characterised at the protein level using 2D-PAGE and tandem mass spectrometry. Comparison of carp muscle from different regions of the fish revealed a higher level of expression of PARV isoforms beta4 and beta5 in the anterior region, which was accompanied by an increase in the rate of relaxation. We postulate that changes in specific PARV isoform expression are an important part of the adaptive change in muscle mechanical properties in response to varying functional demands and environmental change.
authors.affiliation: Faculty of Veterinary Science, University of Liverpool, Liverpool, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: Brownridge
authors.forename: Luciane Vieira
authors.initials: LV
authors.lastname: de Mello
authors.forename: Mary
authors.initials: M
authors.lastname: Peters
authors.forename: Lynn
authors.initials: L
authors.lastname: McLean
authors.forename: Amy
authors.initials: A
authors.lastname: Claydon
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Cossins
authors.forename: Phillip D
authors.initials: PD
authors.lastname: Whitfield
authors.forename: Iain S
authors.initials: IS
authors.lastname: Young
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002347:Carps / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry* / Q000378:metabolism*; D010320:Parvalbumins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D010320:Parvalbumins; D020033:Protein Isoforms