Rapid diagnosis of tuberculous pleuritis by a T-cell interferon-gamma release assay.
To obtain a correct diagnosis of tuberculous pleuritis by analysis of pleural effusions with standard diagnostic tools is difficult. Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunosorbent assay (ELISA) or MTB-specific enzyme-linked immunospot assay (ELISpot) on peripheral blood was not useful because the judgment was indeterminate or negative. However, we recently encountered 2 cases in which tuberculous pleuritis was rapidly diagnosed by performing MTB-specific ELISpot on pleural effusion mononuclear cells (PEMCs), because high levels of MTB-specific cells by ELISpot were demonstrated in pleural effusion of patients with tuberculous pleuritis. to analyze MTB-specific T-cells in pleural effusion mononuclear cells by ELISpot is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of tuberculous pleuritis.
Kobashi
Yoshihiro
Y
Division of Respiratory Diseases, Department of Medicine, Kawasaki Medical School, Kurashiki, Japan. yoshihiro@med.kawasaki-m.ac.jp
Shimizu
Hiroki
H
Mouri
Keiji
K
Obase
Yasushi
Y
Miyashita
Naoyuki
N
Oka
Mikio
M
D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000032:analysis* / Q000378:metabolism; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*; D014396:Tuberculosis, Pleural / Q000175:diagnosis* / Q000188:drug therapy
D000995:Antitubercular Agents; D007371:Interferon-gamma