A sensitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of pharmaceutical indomethacin in water samples.
One-step membrane-based competitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of indomethacin (IDM) in water samples was developed. A positive reaction as a result of the remaining gold-antibody conjugate combining with antigen coated on the membrane was obvious by visual detection. Under optimal conditions, the visual detection limit was found to be 0.1 ngmL(-1), which was about 10-1000 times lower than those in published literatures, demonstrating the high sensitivity of the assay. The stability test indicated the immunochromatographic strips could be used for 8 weeks at room temperature without significant loss of activity. Real water samples were detected by the assay and confirmed by enzyme-linked immunosorbent assay. The assay time for indomethacin detection was less than 10 min, suitable for rapid testing on-site.
Li
Dawei
D
Key Laboratory of Green Chemistry & Technology, Ministry of Education of China, College of Chemistry, Sichuan University, Chengdu 610064, PR China.
Wei
Shuang
S
Yang
Hong
H
Li
Yuan
Y
Deng
Anping
A
D000906:Antibodies / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D003102:Colloids / Q000737:chemistry; D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D007213:Indomethacin / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000906:Antibodies; D003102:Colloids; D014874:Water Pollutants, Chemical; D014867:Water; D006046:Gold; D007213:Indomethacin