L-valine production during growth of pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator SugR.
Pyruvate dehydrogenase complex-deficient strains of Corynebacterium glutamicum produce L-valine from glucose only after depletion of the acetate required for growth. Here we show that inactivation of the DeoR-type transcriptional regulator SugR or replacement of acetate by ethanol already in course of the growth phase results in efficient L-valine production.
Blombach
Bastian
B
Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
Arndt
Annette
A
Auchter
Marc
M
Eikmanns
Bernhard J
BJ
D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D055786:Gene Knockout Techniques; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D014633:Valine / Q000096:biosynthesis*
D011768:Pyruvate Dehydrogenase Complex; D014157:Transcription Factors; D000431:Ethanol; D014633:Valine