An in vitro microfluidic approach to generating protein-interaction networks.
We developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways.
Gerber
Doron
D
Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, 318 Campus Drive, Stanford, CA 94305, USA.
Maerkl
Sebastian J
SJ
Quake
Stephen R
SR
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D040901:Proteomics; D013296:Streptococcus pneumoniae
D001426:Bacterial Proteins