title: [Experimental study on the effect of sensitized sera on engraftment of hematopoietic stem cells].
abstract: OBJECTIVE
  To evaluate the effect of sensitized sera on engraftment of hematopoietic stem cells (HSCs) and its mechanism.
  
  
  METHODS
  Bone marrow cells (BMCs) from C57BL/6 mice were incubated with sensitized sera (group I) or normal sera (group II), and then washed and transplanted into irradiated BALB/c mice. The survival analysis and engraftment evaluation of the recipients were observed. The incubated BMCs were bound with goat anti mouse IgG for and labeled with Annexin V for apoptosis detection.
  
  
  RESULTS
  Seven out of ten recipient mice in group I died of bone marrow failure at day 10 after transplantation, while all of those in group II were long-term survived. Engraftment assay showed recipients blood count and BMCs progressively decreased along with time passing in group I; in addition, the chimeric percentage of donor cells progressively decreased as well. The percentage of BMCs binding with goat anti mouse IgG in group I or group II were (90.3 +/- 5.1)% and (5.2 +/- 2.4)%, respectively, and the difference was statistically significant (P < 0.01). However, no significant difference was found in the apoptosis detection between the two groups (P > 0.05).
  
  
  CONCLUSIONS
  The engraftment capacity of HSCs is significantly impaired by sensitized sera, the antibodies in sensitized sera may bind to antigens expressed on HSCs but do not induce apoptosis.
authors.affiliation: Hematopoietic Stem Cell Transplantation Center, Department of Pediatrics, The Second Affiliated Hospital, SUN Yat-sen University, Guangzhou 510120, China.
authors.forename: Lu-Hong
authors.initials: LH
authors.lastname: Xu
authors.forename: Jian-Pei
authors.initials: JP
authors.lastname: Fang
authors.forename: Hong-Gui
authors.initials: HG
authors.lastname: Xu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Weng
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D018380:Hematopoietic Stem Cell Transplantation; D007106:Immune Sera; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D017304:Annexin A5; D007106:Immune Sera