title: Determination of hair nicotine by gas chromatography-mass spectrometry.
abstract: Hair nicotine is a known biomarker for monitoring long-term environmental tobacco smoke (ETS) exposure and smoking status. In general, hair nicotine assay involves alkaline digestion, extraction and instrumental analysis. The gas chromatography-mass spectrometry (GC-MS) assay currently developed has shown to be of high throughput with average approximately 100 hair samples being extracted and analyzed per day. This was achieved through simplified extraction procedure and shortened GC analysis time. The extraction was improved by using small volume (0.4 mL) of organic solvent that does not require further evaporation and salting steps prior to GC-MS analysis. Furthermore, the amount of hair utilized in the extraction was very little (5 mg) while the sensitivity and selectivity of the assay is equal, if not better than other established methods. The linearity of the assay (r(2)>0.995), limit of quantitation (0.04 ng/mg hair), within- and between-assays accuracies and precisions (<11.4%) and mean recovery (92.6%) were within the acceptable range.
authors.affiliation: National Poison Centre, Universiti Sains Malaysia, 11800 Penang, Malaysia. kaknin337@yahoo.com
authors.forename: Che Nin
authors.initials: CN
authors.lastname: Man
authors.forename: Syazwani
authors.initials: S
authors.lastname: Ismail
authors.forename: Gam Lay
authors.initials: GL
authors.lastname: Harn
authors.forename: Razak
authors.initials: R
authors.lastname: Lajis
authors.forename: Rahmat
authors.initials: R
authors.lastname: Awang
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D016018:Least-Squares Analysis; D009538:Nicotine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012907:Smoking; D014028:Tobacco Smoke Pollution; D055815:Young Adult
chemical_list: D014028:Tobacco Smoke Pollution; D009538:Nicotine