title: Lysophosphatidylcholine induces endothelial cell injury by nitric oxide production through oxidative stress.
abstract: OBJECTIVE
  To determine whether lysophosphatidylcholine (LPC) induces endothelial cell injury by altering the production of nitric oxide (NO) and thereby increasing reactive oxygen species (ROS).
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) were cultured and exposed to LPC, LPC with N(G)-nitro-l-arginine methyl ester (L-NAME), LPC with antioxidants. LPC-induced cell injury and viability were determined using LDH and Resazurin assays. The Mann-Whitney U test was used for statistical analysis.
  
  
  RESULTS
  LPC induced HUVEC injury in a concentration-dependent manner. LPC induced the overproduction of NO and ROS in HUVECs and LPC-induced HUVEC injury is significantly inhibited by the eNOS inhibitor (L-NAME) and the antioxidants (p < 0.05).
  
  
  CONCLUSIONS
  These findings suggest that LPC induces the overproduction of NO, which may increase the oxidative stress on endothelial cells and lead to endothelial cell injury.
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul 158-051, Korea.
authors.forename: Eon A
authors.initials: EA
authors.lastname: Kim
authors.forename: Ji Ae
authors.initials: JA
authors.lastname: Kim
authors.forename: Mi Hye
authors.initials: MH
authors.lastname: Park
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Jung
authors.forename: Suk Hyo
authors.initials: SH
authors.lastname: Suh
authors.forename: Myung-Geol
authors.initials: MG
authors.lastname: Pang
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000975:Antioxidants; D005452:Fluoresceins; D008244:Lysophosphatidylcholines; D009566:Nitrates; D009573:Nitrites; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester