title: The effect of vitamin A on secretion of IFN-gamma and IL-4 in A549 cells induced by Mycoplasma pneumoniae.
abstract: In order to investigate the effect of vitamin A (VA) on the secretion of IFN-gamma and IL-4 in Mycoplasma Pneumoniae (MP)-induced A549 cells, A549 cells were co-cultured with MP for different time lengths and then the levels of IFN-gamma and IL-4 in the cell culture supernatants were detected before and after treatment with different concentrations of VA by using the enzyme-linked immunosorbent assay (ELISA). The results showed that the level of IFN-gamma and IL-4 in the supernatants of MP-induced A549 cells was much higher than that in non-induced cells (P<0.01). After application of VA, IL-4 level was not increased until the concentration of VA was up to 0.5x10(-5) mol/L (P<0.01). However, with concentration of VA increased up to 1x10(-4) mol/L, IL-4 was significantly suppressed (P<0.01). It was concluded that MP could induce the secretion of IFN-gamma and IL-4 in A549 cells. VA could inhibit the secretion of IFN-gamma and increase the IL-4 level in MP-induced A549 cells. However, high concentration of VA had an inhibitory effect on the secretion of IL-4 as well as on the IFN-gamma. These data provided a theoretical basis for the application of VA in MP pneumonia in the clinical practice.
authors.affiliation: Department of Microbiology, School of Basic Medicines, Wuhan University, Wuhan, 430071, China. xiaolanwu@126.com
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Wu
authors.forename: Xianzhou
authors.initials: X
authors.lastname: Liu
authors.forename: Jilu
authors.initials: J
authors.lastname: Tang
mesh_terms: D045744:Cell Line, Tumor; D018920:Coculture Techniques; D046508:Culture Techniques; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D008175:Lung Neoplasms / Q000473:pathology; D009177:Mycoplasma pneumoniae / Q000254:growth & development / Q000502:physiology*; D014801:Vitamin A / Q000494:pharmacology*
chemical_list: D014801:Vitamin A; D015847:Interleukin-4; D007371:Interferon-gamma