[Effects of rhizoma paridis total saponins on levels of cytokines in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide].
OBJECTIVE
To investigate the effect of rhizoma paridis total saponins(RPTS)on the levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide.


METHODS
Sixty-eight Wistar rats were randomly divided into five groups. The models were made in four groups (expect the blank control group) in accordance with the standard of two-hit animal model induced by multiple fractures and lipopolysaccharide. At 1 hour after models made,the rats in RPTS groups were given rhizoma paridis total saponins with different concentrations by intragastric administration. Six hours later, the concentrations of TNF-alpha, IL-1 beta and IL-6 in the blood serum of all rats were detected by ELISA (enzyme linked immunosorbent assay).


RESULTS
The concentrations of TNF-alpha, IL-1 beta and IL-6 in blood serum of rats in the model group were remarkably higher than those in the blank control group (P<0.001), and these in the RPTS groups were remarkably lower than those in the model group (P<0001).


CONCLUSIONS
RPTS can decrease the levels of TNF-alpha and IL-6 and IL-1 beta in the blood serum of rats subjected to two-hit induced by multiple fractures and lipopolysaccharide.
Zhou
Man-hong
MH
Department of Emergency, the Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou, China. manhongzhou@sina.com
He
Hua-jing
HJ
Pan
Yong
Y
Shan
Li-jing
LJ
Ma
Yi
Y
Liu
Yang
Y
D000818:Animals; D016207:Cytokines / Q000097:blood*; D005260:Female; D050723:Fractures, Bone / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D009104:Multiple Trauma / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use
D016207:Cytokines; D008070:Lipopolysaccharides; D012503:Saponins