Continuous on-site label-free ATP fluorometric assay based on aggregation-induced emission of silole.
In this letter, we have described the establishment of a label-free fluorometric assay to detect adenosine triphosphate (ATP), which can enable the monitoring of the ATP hydrolysis process continuously and in situ. This assay is based on a silole derivative with positively charged modifications, compound 1, that can aggregate on the negative charged ATP template through charge-charge interaction. Because the silole group has strong aggregation-induced fluorescence emission (AIE), we have found that the aggregation could induce strong fluorescence emission and its intensity is positively and linearly related to the ATP concentration. Meanwhile, 1 also shows good discrimination for ATP, where its hydrolysis products ADP, AMP, and adenosine could not induce sufficient aggregation to produce a strong fluorescence signal. These characteristics are sufficient to build up a sensitive fluorescence assay to monitor the ATP concentration change in solution in situ, and we have also verified its usability in studying the reaction process of phosphatase.
Zhao
Manchun
M
National Centre for Nanoscience and Technology, No. 11, Beiyitiao, Zhongguancun, Beijing 100190, PR China.
Wang
Ming
M
Liu
Huajie
H
Liu
Dongsheng
D
Zhang
Guanxin
G
Zhang
Deqing
D
Zhu
Daoben
D
D000255:Adenosine Triphosphate / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D002417:Cattle; D006868:Hydrolysis; D007422:Intestines / Q000201:enzymology; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology
D012821:Silanes; D014638:Vanadates; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase