title: Developing a novel model system to target insulin-reactive CD8 T cells.
abstract: CD8 T cells play an important role in autoimmune diabetes development, and therefore removing these cells may protect against disease. To test this, we designed a novel method using engineered cells (InsCD3-zeta) to target insulin-specific CD8 T cells. Insulin-reactive target cells were cultured with InsCD3-zeta CD8 T cells and cytotoxicity was assessed. Activated, but not naïve, InsCD3-zeta CD8 T cells readily killed insulin-reactive target CD8 T cells. This approach to immunotarget relevant pathogenic CD8 T cells may be a therapeutic option to delay or prevent type 1 diabetes.
authors.affiliation: Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: Gwen S
authors.initials: GS
authors.lastname: Scott
authors.forename: Sigal
authors.initials: S
authors.lastname: Fishman
authors.forename: Alon
authors.initials: A
authors.lastname: Margalit
authors.forename: L Khai
authors.initials: LK
authors.lastname: Siew
authors.forename: Stephen
authors.initials: S
authors.lastname: Chapman
authors.forename: Li
authors.initials: L
authors.lastname: Wen
authors.forename: Gideon
authors.initials: G
authors.lastname: Gross
authors.forename: F Susan
authors.initials: FS
authors.lastname: Wong
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000235:genetics; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D003603:Cytotoxins / Q000494:pharmacology / Q000627:therapeutic use*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D007328:Insulin / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic
chemical_list: D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D003603:Cytotoxins; D007328:Insulin