Induction of iodide uptake in transformed thyrocytes: a compound screening in cell lines.
OBJECTIVE
Retinoic acid presently is the most advanced agent able to improve the efficacy of radioiodine therapy in differentiated thyroid carcinoma. In order to identify compounds with higher efficacy a panel of pharmacologically well-characterized compounds with antitumour action in solid cancer cell lines was screened.


METHODS
The effects of the compounds on iodide uptake, cell number, proliferation and apoptosis were evaluated.


RESULTS
In general, compounds were more effective in cell lines derived from more aggressive tumours. The effectiveness in terms of number of responsive cell lines and maximal increase in iodide uptake achieved decreased in the order: APHA > valproic acid approximately sirolimus approximately arsenic trioxide > retinoic acid approximately lovastatin > apicidine approximately azacytidine approximately retinol approximately rosiglitazone approximately bortezomib.


CONCLUSIONS
We hypothesize that testing of cells from primary tumours or metastases in patients may be a way to identify compounds with optimum therapeutic efficacy for individualized treatment.
Fröhlich
Eleonore
E
Department of Endocrinology, Metabolism, Nephrology and Clinical Chemistry, Internal Medicine, University of Tuebingen, Tuebingen, Germany.
Brossart
Peter
P
Wahl
Richard
R
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology; D001897:Boronic Acids / Q000494:pharmacology; D000069286:Bortezomib; D002277:Carcinoma / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007454:Iodides / Q000378:metabolism*; D008148:Lovastatin / Q000494:pharmacology; D009362:Neoplasm Metastasis; D010087:Oxides / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology; D020123:Sirolimus / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*; D014212:Tretinoin / Q000378:metabolism
D000970:Antineoplastic Agents; D001152:Arsenicals; D001897:Boronic Acids; D007454:Iodides; D010087:Oxides; D011719:Pyrazines; D014212:Tretinoin; D000069286:Bortezomib; D008148:Lovastatin; D000077237:Arsenic Trioxide; D020123:Sirolimus