[Fenofibrate improves energy metabolism and attenuates isoproterenol induced acute myocardial ischemic injury in rats via PPAR alpha activation].
OBJECTIVE
To observe the effects of peroxisome proliferator-activated receptor (PPAR) alpha agonist Fenofibrate (FF) on energy metabolism and histology in isoproterenol (Iso) induced acute myocardial ischemic injury model.


METHODS
Male Wistar rats were randomly divided into control group, Iso group (5 mg/kg, i.p.) and FF group (80 mgxkg(-1)xd(-1) per gavage for 7 days, then Iso 5 mg/kg, i.p. n = 30 each). Twenty-four hours post Iso, heart weight/body weight ratio, myocardial histopathological changes (HE staining), serum and myocardial free fatty acids (FFA) levels, the myocardial protein expression of PPARalpha (Western blot) were determined.


RESULTS
Compared with the control group, pathological myocardial injuries were observed under light microscope in Iso treated hearts and FF pretreatment could significantly attenuate these changes [necrotic area: 0 vs (10.00 +/- 3.00)% vs (7.36 +/- 2.60)%], the heart weight/body weight ratio, FFA in serum (501.17 +/- 43.69 vs 939.53 +/- 69.51 vs 736.53 +/- 70.30 micromol/L) and myocardium (62.01 +/- 9.19 vs 140.59 +/- 19.34 vs 116.28 +/- 14.03 micromol/L) were significantly increased while myocardial protein expressions of PPARalpha (251.57 +/- 10.95 vs 191.97 +/- 10.74 vs 215.08 +/- 9.61) was significantly downregulated in the Iso group and FF pretreatment could significantly attenuate these changes (all P < 0.05).


CONCLUSIONS
Our data suggested that the FFA utilization was decreased in Iso induced acute myocardial ischemic injury and FF could attenuate Iso induced myocardial damage via activating PPARalpha signaling pathway.
Yuan
Jie
J
Department of Cardiology, Second Hospital of Harbin Medical University, Harbin 150086, China.
Wu
Jian
J
Han
Zhi-Gang
ZG
D000818:Animals; D004195:Disease Models, Animal; D004734:Energy Metabolism; D011345:Fenofibrate / Q000494:pharmacology* / Q000627:therapeutic use; D007545:Isoproterenol; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D015428:Myocardial Reperfusion Injury / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D009206:Myocardium / Q000378:metabolism*; D047493:PPAR alpha / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D047493:PPAR alpha; D007545:Isoproterenol; D011345:Fenofibrate