title: Dual DNA vaccination of rainbow trout (Oncorhynchus mykiss) against two different rhabdoviruses, VHSV and IHNV, induces specific divalent protection.
abstract: DNA vaccines encoding the glycoprotein genes of the salmonid rhabdoviruses VHSV and IHNV are very efficient in eliciting protective immune responses against their respective diseases in rainbow trout (Oncorhynchus mykiss). The early anti-viral response (EAVR) provides protection by 4 days post vaccination and is non-specific and transient while the specific anti-viral response (SAVR) is long lasting and highly specific. Since both VHSV and IHNV are endemic in rainbow trout in several geographical regions of Europe and Atlantic salmon (Salmo salar) on the Pacific coast of North America, co-vaccination against the two diseases would be a preferable option. In the present study we demonstrated that a single injection of mixed DNA vaccines induced long-lasting protection against both individual and a simultaneous virus challenge 80 days post vaccination. Transfected muscle cells at the injection site expressed both G proteins. This study confirms the applied potential of using a combined DNA vaccination for protection of fish against two different rhabdoviral diseases.
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Hangøvej 2, DK-8200 Arhus N, Denmark. kaei@vet.dtu.dk
authors.forename: Katja
authors.initials: K
authors.lastname: Einer-Jensen
authors.forename: Lourdes
authors.initials: L
authors.lastname: Delgado
authors.forename: Ellen
authors.initials: E
authors.lastname: Lorenzen
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bovo
authors.forename: Øystein
authors.initials: Ø
authors.lastname: Evensen
authors.forename: Scott
authors.initials: S
authors.lastname: Lapatra
authors.forename: Niels
authors.initials: N
authors.lastname: Lorenzen
mesh_terms: D000818:Animals; D005060:Europe; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D029142:Infectious hematopoietic necrosis virus / Q000235:genetics / Q000276:immunology*; D009656:North America; D029141:Novirhabdovirus / Q000235:genetics / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000662:veterinary*; D020317:Salmo salar; D016019:Survival Analysis; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D019444:Vaccines, DNA; D014765:Viral Vaccines