title: Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain.
abstract: In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections.
authors.affiliation: CICVyA-INTA Castelar, Buenos Aires, Argentina.
authors.forename: Rosalía
authors.initials: R
authors.lastname: Moretta
authors.forename: Paula
authors.initials: P
authors.lastname: Ruybal
authors.forename: María
authors.initials: M
authors.lastname: Mesplet
authors.forename: Romina
authors.initials: R
authors.lastname: Petrigh
authors.forename: Pablo
authors.initials: P
authors.lastname: Nuñez
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Gil
authors.forename: Silvina
authors.initials: S
authors.lastname: Wilkowsky
authors.forename: Graciela
authors.initials: G
authors.lastname: Garbossa
authors.forename: Marisa
authors.initials: M
authors.lastname: Farber
mesh_terms: D042322:Anaplasma marginale / Q000302:isolation & purification*; D000818:Animals; D004912:Erythrocytes / Q000382:microbiology; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015203:Reproducibility of Results; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes