Characterization of chemoreceptive protein binding to an oviposition stimulant using a fluorescent micro-binding assay in a butterfly.
A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated previously from the chemoreceptive organs was assumed to bind to an oviposition stimulant. Using a highly sensitive fluorescent micro-binding assay, we clarified OSBP bound to aristolochic acid. Three-dimensional molecular modeling revealed the structure of the OSBP-aristolochic acid complex. This is the first report of a native chemoreceptive protein binding to an oviposition stimulant as a ligand in insects.
Tsuchihara
Kazuko
K
Iwaki Meisei University, Faculty of Pharmacy, 5-5-1 Iino, Chuo-dai, Iwaki, Fukushima 970-8551, Japan. tutihara@iwakimu.ac.jp
Wazawa
Tetsuichi
T
Ishii
Yoshiharu
Y
Yanagida
Toshio
T
Nishida
Ritsuo
R
Zheng
Xiang-Guo
XG
Ishiguro
Masaji
M
Yoshihara
Kazuo
K
Hisatomi
Osamu
O
Tokunaga
Fumio
F
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D030022:Aristolochia / Q000378:metabolism; D034341:Aristolochic Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001681:Biological Assay; D002080:Butterflies / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D010058:Oviposition / Q000187:drug effects; D011487:Protein Conformation
C122155:6-bromoacetyl-2-dimethylaminonaphthalene; D034341:Aristolochic Acids; D005456:Fluorescent Dyes; D019476:Insect Proteins; C000228:aristolochic acid I; D015081:2-Naphthylamine