title: Immunoreactivity of five antigens of Mycobacterium tuberculosis in patients attending a public health care facility in an area with high endemicity for TB.
abstract: The objective of this study was to evaluate people attending a primary health clinic in Rio de Janeiro, Brazil for immunoreactivity to five Mycobacterium tuberculosis antigens, as these antigens are markers of immune response and factors associated with active TB. The serum antibody titers of different categories of patients (defined by microbiological and radiological characteristics and by response to therapy on follow-up) to 38 kDa, 16 kDa, MPT64, ESAT-6 and MT10.3 antigens were determined blind with ELISA. Positive tests to each antigen were defined with ROC analysis. OR were calculated for factors associated with humoral response in patients with active TB. A total of 201 patients underwent serological testing. Patients with confirmed active TB responded more frequently to MPT64 (44%), 16 kDa (37.7%) and 38 kDa (36.1%). ESAT-6 and MT10.3 were also able to distinguish people in TB groups from controls. TB infected subjects responded less frequently to ESAT-6 and MT10.3 (3.7% and 11%, respectively). Sensitivity and specificity to all antigens combined were 58.4% and 60.7%, respectively. Reactivity to 38 kDa and to MPT64 was more likely among alcohol users OR 2.61 (95%CI;1.05-6.94) and OR 3.27 (95%CI;1.33-8.15), respectively. 16 kDa antigen elicited a more protective response among smokers, OR 0.29 (95%CI; 0.10-0.83). It was concluded that reactivity to all antigens tested represented markers of active disease. ESAT-6 and MT10.3 could not be identified as markers of TB infection in this community. Sensitivity was higher to all antigens combined, but at a cost of lower specificity. Interestingly, among factors associated with positive immunoreactivity, alcohol use and smoking seem to polarize the humoral response in different directions. This finding deserves further investigation.
authors.affiliation: Faculty of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Vania Maria C
authors.initials: VM
authors.lastname: Silva
authors.forename: Isabela Gama
authors.initials: IG
authors.lastname: Sardella
authors.forename: Ronir Raggio
authors.initials: RR
authors.lastname: Luiz
authors.forename: Antonio José Ledo A
authors.initials: AJ
authors.lastname: Cunha
authors.forename: Aline H
authors.initials: AH
authors.lastname: Cavalcanti
authors.forename: Singh
authors.initials: S
authors.lastname: Mahavir
authors.forename: Miriam Menna
authors.initials: MM
authors.lastname: Barreto
authors.forename: Rosana Souza
authors.initials: RS
authors.lastname: Rodrigues
authors.forename: Thaís Ferrão
authors.initials: TF
authors.lastname: Carvalho
authors.forename: Maria Helena Féres
authors.initials: MH
authors.lastname: Saad
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010044:Outpatient Clinics, Hospital; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015415:Biomarkers; C072681:Mycobacterium tuberculosis antigens