title: In vitro plant regeneration from direct and indirect organogenesis of Memordica charantia.
abstract: The study was conducted to analyze the effect of plant growth regulators on callogenesis and direct and indirect organogenesis of Memordica charantia. Callus cultures were induced from leaf, stem and cotyledonary explants of Momordica charantia, at different auxin and cytokinin concentrations either in single or in combination in MS medium. The best callogenic response was observed from all three explants (leaf, stem and cotyledon) on MS medium supplemented with 1.0 and 1.5 mg L(-1) BAP with 1.5 mg L(-1) NAA and 1.0 mg L(-1) 2,4-D, respectively. The callus produced was hard, green and compact. These totipotent cells were failed to give rise shooting response when transferred to same or different growth regulator containing medium as second subculture. Indirect organogenesis response was very low or absent due to hardening of callus and habitutation. Best shooting was observed at 1.0 mg L(-1) BAP + 0.1 TDZ and 1.5 mg L(-1) BAP + 0.2 mg L(-1) NAA from shoot tip and cotyledonary node explants. While root formation was achieved when generated shoots were transferred to MS medium both full and half strength supplemented with different auxin concentrations.
authors.affiliation: Department of Biotechnology, Faculty of Biological Sciences, Plant Cell and Tissue Culture Lab, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Saima
authors.initials: S
authors.lastname: Malik
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zia
authors.lastname: Riaz-ur-Rehman
authors.forename: M Fayyaz
authors.initials: MF
authors.lastname: Chaudhary
mesh_terms: D019662:Cucurbitaceae / Q000502:physiology*; D003583:Cytokinins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D010937:Plant Growth Regulators; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D018520:Plant Shoots; D010946:Plants, Medicinal / Q000378:metabolism; D011336:Probability; D012038:Regeneration; D036226:Seedlings / Q000502:physiology; D046509:Tissue Culture Techniques
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators