Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine (TCM) liriodenine with metal ions, and DNA binding studies.
Liriodenine (), an active component of the anticancer traditional Chinese medicine (TCM), was isolated from Zanthoxylum nitidum. Its reactions with Pt(II) and Ru(II) afforded three metal complexes: cis-[PtCl2(L)] (), cis-[PtCl2(L)(DMSO)] (), and cis-[RuCl2(L)(DMSO)2].1.5H2O (), the crystal structures of , and were determined by single-crystal X-ray diffraction methods. These complexes were fully characterized by elemental analysis, IR spectrophotometry, 1H and 13C NMR spectroscopies, and ES mass spectrometry. The in vitro cytotoxicity of and complexes against 11 human tumour cell lines was assayed. The metal-based compounds exhibit enhanced cytotoxicity vs. free , suggesting that these compounds display synergy in the combination of metal ions and liriodenine. The binding properties of and its complexes to ct-DNA were investigated through UV-vis, fluorescence, CD spectra, viscosity and agarose gels electrophoretic measurements.
Chen
Zhen-Feng
ZF
The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry & Chemical Engineering of Guangxi Normal University, Guilin 541004, P. R. China. chenzfubc@yahoo.com
Liu
Yan-Cheng
YC
Liu
Li-Min
LM
Wang
Heng-Shan
HS
Qin
San-Hai
SH
Wang
Bo-Long
BL
Bian
He-Dong
HD
Yang
Bin
B
Fun
Hoong-Kun
HK
Liu
Hua-Gang
HG
Liang
Hong
H
Orvig
Chris
C
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001060:Aporphines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D017671:Platinum Compounds / Q000737:chemistry* / Q000494:pharmacology; D017975:Ruthenium Compounds / Q000737:chemistry* / Q000494:pharmacology; D032106:Zanthoxylum / Q000737:chemistry*
D000970:Antineoplastic Agents; D001060:Aporphines; D017671:Platinum Compounds; D017975:Ruthenium Compounds; D004247:DNA; C026980:liriodenine