A rice kinase-protein interaction map.
Plants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function. We previously developed a cost-effective high-throughput yeast two-hybrid system. Using this system, we have generated a protein interaction map of 116 representative rice kinases and 254 of their interacting proteins. Overall, the resulting interaction map supports a large number of known or predicted kinase-protein interactions from both plants and animals and reveals many new functional insights. Notably, we found a potential widespread role for E3 ubiquitin ligases in pathogen defense signaling mediated by receptor-like kinases, particularly by the kinases that may have evolved from recently expanded kinase subfamilies in rice. We anticipate that the data provided here will serve as a foundation for targeted functional studies in rice and other plants. The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed.
Ding
Xiaodong
X
Department of Plant Pathology , University of Florida, Gainesville, Florida 32611, USA.
Richter
Todd
T
Chen
Mei
M
Fujii
Hiroaki
H
Seo
Young Su
YS
Xie
Mingtang
M
Zheng
Xianwu
X
Kanrar
Siddhartha
S
Stevenson
Rebecca A
RA
Dardick
Christopher
C
Li
Ying
Y
Jiang
Hao
H
Zhang
Yan
Y
Yu
Fahong
F
Bartley
Laura E
LE
Chern
Mawsheng
M
Bart
Rebecca
R
Chen
Xiuhua
X
Zhu
Lihuang
L
Farmerie
William G
WG
Gribskov
Michael
M
Zhu
Jian-Kang
JK
Fromm
Michael E
ME
Ronald
Pamela C
PC
Song
Wen-Yuan
WY
D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
D010940:Plant Proteins; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; D011494:Protein Kinases; D047390:Casein Kinase II; D018844:Cyclin-Dependent Kinases; D020928:Mitogen-Activated Protein Kinases