An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity.
The high number of peroxidase genes explains the description of numerous physiological functions and the fact that the in planta function of a single isoform has never been characterized yet. We analyzed in transgenic Arabidopsis thaliana the localization of a zucchini isoperoxidase (APRX), previously purified thanks to its pectin binding ability. We confirmed that the protein is localized near the cell wall, mainly produced in the elongation area of the hypocotyls and respond to exogenous auxin. In addition, the ectopic overexpression of APRX induced changes in growth pattern and a significant reduction of endogenous indole-3-acetic acid (IAA) level. In agreement with these observations APRX showed an elevated in vitro auxin oxidase activity. We propose that APRX participates in the negative feedback regulation of auxin level and consequently terminates the hypocotyl elongation process.
Cosio
Claudia
C
Laboratoire of Plant Biochemistry and Physiology, University of Geneva, Quai Ernest-Ansermet 30, 1211, Geneva 4, Switzerland.
Vuillemin
Loic
L
De Meyer
Mireille
M
Kevers
Claire
C
Penel
Claude
C
Dunand
Christophe
C
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D016000:Cluster Analysis; D028464:Cucurbita / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018546:Hypocotyl / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D007210:Indoleacetic Acids / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism*; D010544:Peroxidases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D007210:Indoleacetic Acids; D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D015084:2,4-Dichlorophenoxyacetic Acid; D010088:Oxidoreductases; D010544:Peroxidases; C120494:anionic peroxidase