Calcification by MC3T3-E1 cells on RGD peptide immobilized on titanium through electrodeposited PEG.
The effect of a cell-adhesive peptide containing Arg-Gly-Asp (RGD) immobilized through poly(ethylene glycol) (PEG) on titanium (Ti) on calcification by MC3T3-E1 cells was investigated to develop a new surface modification technique using biofunctional molecules. RGD was immobilized on Ti through PEG, both terminals of which were terminated with -NH(2) and -COOH to combine with the Ti surface and RGD. PEG was immobilized on Ti with electrodeposition, and RGD, with immersion. For comparison, glycine was employed because it is the simplest molecule containing both -NH(2) and -COOH at its terminals. MC3T3-E1 cells were cultured and differentiation-induced on each specimen, and the cell calcification properties were investigated. As a result, there was no significant difference in the morphology and extension of MC3T3-E1 cells cultured on each specimen, while the number of cells cultured on RGD/PEG/Ti was the largest. After differentiation-induction, there was no significant difference in the ALP activity among all specimens. On the other hand, the level of cell calcification on RGD/PEG/Ti was the highest. Therefore, the hard tissue compatibility of Ti is improved by immobilizing RGD through functional molecules which have a long molecular chain.
Oya
Kei
K
Department of Metals, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Tanaka
Yuta
Y
Saito
Haruka
H
Kurashima
Kazuya
K
Nogi
Kazuya
K
Tsutsumi
Harumi
H
Tsutsumi
Yusuke
Y
Doi
Hisashi
H
Nomura
Naoyuki
N
Hanawa
Takao
T
D016475:3T3 Cells / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium; D000469:Alkaline Phosphatase