Anti-plasminogen monoclonal antibody (MC2B8) inhibites angiogenesis.
Angiogenesis is a complex process during which of new blood vessels are produced from the preexisting blood vessels. Formation and growth of new vessels play an important role in the physiologic process (embryonic growth, tissue repair) and pathologic process (tumor growth, inflammation) for surviving of the tissues. In fact, the development of tumors is depended upon new vessel formation through which the tumor is provided with nutrient and oxygen. In this research, the role ofplasminogen conformation with MC2B8 mAb (an antibody directed against C-terminal part of plasminogen) in clot lysis and angiogenesis is observed. In experimental model of angiogenesis, beads, covered with endothelial cells of bone marrow capillaries, are the source of endothelial cells. It coated in three-dimensional structure to be provided through fibrin gel. Different titers of monoclonal antibody (30-480 microg mL(-1)) MC2B8 were added in fibrin gel. 3-5 days after culturing of endothelial cells, growth and migration was seen as the result of capillary formation MC2B8 mAb delayed clot lysis and inhibited angiogenesis at the concentration of 240 microg mL(-1). Present findings suggest that these effects on capillary tube formation and clot lysis caused blockage or conformational changes in plasminogen epitopes involved in angiogenesis and fibrinolysis.
Mansouri
K
K
Kermanshah Medical Biology Research Center, University of Medical Sciences, Kermanshah, P.O. Box 14115-111, Tehran, Islamic Republic of Iran.
Mirshahi
M
M
Pourfathollah
A
A
Hassan
Z M
ZM
Taheripak
R
R
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000276:immunology*; D010958:Plasminogen / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010958:Plasminogen