Determination of viable mammalian cells by luminol chemiluminescence using microperoxidase.
Chemiluminescent assay for menadione-catalyzed H(2)O(2) production by mammalian cells was modified by luminol chemiluminescence with microperoxidase instead of peroxyoxalate chemiluminescence with carcinogenic fluorescent materials. Luminol can be used as a common chemiluminescent reagent for the determination of viable mammalian cells and bacteria.
Yamashoji
Shiro
S
Shizuoka Institute of Science and Technology, Fukuroi, Shizuoka, Japan. yamasho@ms.sist.ac.jp
D000818:Animals; D002452:Cell Count / Q000379:methods*; D002470:Cell Survival; D006801:Humans; D006861:Hydrogen Peroxide; D008163:Luminescent Measurements; D008165:Luminol; D008722:Methods; D010544:Peroxidases; D012107:Research Design; D024483:Vitamin K 3
D008165:Luminol; D024483:Vitamin K 3; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021799:microperoxidase