DNA gains and losses of chromosome in laryngeal squamous cell carcinoma using comparative genomic hybridization.
OBJECTIVE
A larynx squamous cell carcinoma (LSCC) is one of the most common forms of cancer and may exhibit various complex karyotypes.


METHODS
We used comparative genomic hybridization (CGH) to analyze DNA gains and losses in 15 squamous cell carcinomas that consisted of 4 glottic, 10 supraglottic, and 1 transglottic localization samples.


RESULTS
The majority of the chromosomal alterations detected were gains: 3 samples of LSCCs revealed high level amplification, while 6 samples displayed gains in various chromosomal regions (17p, 3p, 4p, 5p, 6q, 8p, 9p, 14q, 18p and Xq). One sample was found to have losses (chromosomes 15q and 22q) and 5 had normal CGH profiles.


CONCLUSIONS
Many of these gained regions (4p, 5p, 8p, 10q, 18q and Xq) were novel sites, which may harbor oncogene(s) that potentially play an important role in squamous cell tumorigenesis and progression at supraglottic localizations.
Keser
Ibrahim
I
Department of Medical Genetics, School of Medicine, Akdeniz University, TR-07070, Antalya, Turkey. keser@akdeniz.edu.tr
Toraman
Ahter D
AD
Ozbilim
Gulay
G
Guney
Kenan
K
Luleci
Guven
G
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007621:Karyotyping; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D004273:DNA, Neoplasm