title: Detection and chromatographic removal of lipopolysaccharide in preparations of multifunctional galectins.
abstract: The functional spectrum of human galectins is currently explored, with a wide range of activities being described. The role of galectin-3 as adhesin for bacteria is based on its strong binding to lipopolysaccharides (LPSs), which brings the possibility of such a contamination in galectin preparations to awareness. This assumption was verified in three independent functional assay systems using polymyxin B as inhibitor of LPS-dependent effects. Moreover, a commercial LPS quantification kit also revealed LPS in galectin preparations. Chromatography was effective in removing LPS, suggesting that such a technique needs to be applied to prevent assigning cellular responses to galectins rather than LPS.
authors.affiliation: Department of Internal Medicine 3, Friedrich-Alexander University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Sarter
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Herbert
authors.initials: H
authors.lastname: Kaltner
authors.forename: Martin
authors.initials: M
authors.lastname: Lensch
authors.forename: Connie
authors.initials: C
authors.lastname: Schulze
authors.forename: Vilma
authors.initials: V
authors.lastname: Urbonaviciute
authors.forename: Georg
authors.initials: G
authors.lastname: Schett
authors.forename: Martin
authors.initials: M
authors.lastname: Herrmann
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
mesh_terms: D000818:Animals; D002460:Cell Line; D002845:Chromatography; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism; D037502:Galectin 3 / Q000096:biosynthesis / Q000494:pharmacology; D037161:Galectins / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D011112:Polymyxin B / Q000494:pharmacology; D011485:Protein Binding; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000737:chemistry* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D037502:Galectin 3; D037161:Galectins; D008070:Lipopolysaccharides; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011112:Polymyxin B