Sterilization of Bacillus pumilus spores using supercritical fluid carbon dioxide containing various modifier solutions.
Supercritical fluid carbon dioxide (SF-CO(2)) with small amounts of chemical modifier(s) provides a very effective sterilization technique that should be useful for destroying microorganism on heat-sensitive devices such as instruments flown on planetary-bound spacecraft. Under a moderate temperature (50 degrees C) and pressure (100 atm), spores of Bacillus pumilus strains ATCC 7061 and SAFR 032 can be effectively inactivated/eliminated from metal surfaces and small electronic devices in only 45 min using optimized modifier concentrations. Modifiers explored in this study included hydrogen peroxide (H(2)O(2)), tert-butyl hydroperoxide, formic acid, and Triton X-100. During sterilization procedure the modifiers were continuously added to SF-CO(2) in either methanol or water at controlled concentrations. The lowest effective concentrations were established for each modifier. Complete elimination of both types of B. pumilus endospores occurred with an optimal modifier addition of either or 10% methanol containing 12% H(2)O(2) or 12% tert-butyl hydroperoxide in SF-CO(2), or a mixture of 6% H(2)O(2) and 6% tert-butyl hydroperoxide. Using water as the carrier of SF-CO(2) modifier, the complete elimination of spores viability of both B. pumilus strains occurred with an addition of either 3.3% water containing 3% H(2)O(2), or 3.3% water containing 10% methanol and 0.5% formic acid, or 3.3% water containing 10% methanol, 1% formic acid and 2% H(2)O(2).
Shieh
Edison
E
Environmental Biotechnology Institute, University of Idaho, Moscow, Idaho 83844, United States; Department of Microbiology Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844, United States.
Paszczynski
Andrzej
A
Wai
Chien M
CM
Lang
Qingyong
Q
Crawford
Ronald L
RL
D001407:Bacillus / Q000187:drug effects* / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology*; D005561:Formates / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017830:Octoxynol / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D005561:Formates; C030544:formic acid; D002245:Carbon Dioxide; D017830:Octoxynol; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide