title: Genetic diversity and zoonotic potential of human rotavirus strains, 2003-2006, Hungary.
abstract: Rotavirus strain surveillance is being conducted in many countries before and after introduction of newly licensed vaccines to assess the impact of the vaccines on rotavirus strains. Here we describe a strain surveillance study in the Budapest area of Hungary (2003-2006) based on RNA profile analysis, genotyping by multiplex PCR and nucleotide sequencing. Among 1,983 G-typed rotaviruses we identified G1 (22%), G2 (4.8%), G3 (3.5%), G4 (18.5%), G6 (1.1%), G8 (<0.1%, n = 1), G9 (42%), and G12 (3.4%) specificities. Information on P genotype incidence was determined for a subset of samples (n = 814). In addition to the globally important strains, a variety of uncommon antigen combinations were also found, for example, P[9],G3; P[14],G6; or P[14],G8. Sequence and phylogenetic analysis of the VP7, VP4, VP6, and NSP4 genes of selected strains with uncommon antigen combinations demonstrated high similarity with certain bovine, porcine, feline, equine, and lapine rotaviruses, respectively. Continued surveillance is needed to assess the role of animal rotaviruses in human diseases.
authors.affiliation: Regional Laboratory of Virology, Baranya County Institute of State Public Health Service, Pécs, Hungary. bkrota@hotmail.com
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.forename: Agnes
authors.initials: A
authors.lastname: Bogdán
authors.forename: Gertrud
authors.initials: G
authors.lastname: Domonkos
authors.forename: Péter
authors.initials: P
authors.lastname: Kisfali
authors.forename: Péter
authors.initials: P
authors.lastname: Molnár
authors.forename: András
authors.initials: A
authors.lastname: Tóth
authors.forename: Béla
authors.initials: B
authors.lastname: Melegh
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Jon R
authors.initials: JR
authors.lastname: Gentsch
authors.forename: György
authors.initials: G
authors.lastname: Szucs
mesh_terms: D000293:Adolescent; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D015047:Zoonoses / Q000821:virology*
chemical_list: D000956:Antigens, Viral