title: [Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain].
abstract: OBJECTIVE
  To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain.
  
  
  METHODS
  Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.
  
  
  RESULTS
  The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.
  
  
  CONCLUSIONS
  Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.
authors.affiliation: Agriculture Department of Jilin University, Changchun 130062, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D011818:Rabies / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins