title: [Research on application of modified polyethylene glycol hydrogels in the construction of tissue engineered heart valve].
abstract: OBJECTIVE
  To explore the effect of the polyethylene glycol (PEG)-hydrogels to enhance the seeding-cells adhesion to the biomaterial scaffolds.
  
  
  METHODS
  Sixteen porcine aortic valves were decellularized with Triton X-100 and trypsin, then divided into A and B group, eight in each group. Group A: the donor goat's autologous bone marrow mesenchymal stem cells (BMSCs) Selected as the seeding-cells were encapsulated into the modified PEG-hydrogels to complete the process of the cells attaching to the acellular porcine aortic valves. Non-PEG but reservation of BMSCs was modified in Group B. After static culture for 7 d, the mono semilunar tissue engineering heart valve (TEHV) were implanted respectively into each donor goat's abdominal aortas. Gross and histology examination, ultrasonic scanning, electron microscopy observation and biomechanics detection were performed at 16 weeks after operation. The 8 native goat aortic valves from the donor goats were selected at the same time as control group (Group C).
  
  
  RESULTS
  There were much more improvements compared Group A to Group B (P < 0.05) in tensile strength [(12.9 +/- 1.3) MPa vs. (8.8 +/- 0.4) MPa], ratio of re-endothelial (84.6% vs. 14.8%) and mural thrombosis (0/8 vs. 8/8). The data illustrated the critical importance of BMSCs differentiation to endothelial and myofibroblast for remodeling into native tissue in microenvironment in vivo.
  
  
  CONCLUSIONS
  It is feasible to reconstruct TEHV efficiently by combining modified PEG-hydrogels with acellular biomaterial scaffold and autologous MSCs cells. It can improve the integration of the seeding-cells and scaffold. It can also protect the growth and differentiation of the BMSCs in the systemic circulation effectively.
authors.affiliation: Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ouyang
authors.forename: Jin-bao
authors.initials: JB
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yang-hong
authors.initials: YH
authors.lastname: Peng
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Kang
authors.forename: Ying-mei
authors.initials: YM
authors.lastname: Wang
authors.forename: Xu-feng
authors.initials: XF
authors.lastname: Wei
authors.forename: Ding-hua
authors.initials: DH
authors.lastname: Yi
authors.forename: Wei-yong
authors.initials: WY
authors.lastname: Liu
mesh_terms: D000818:Animals; D001021:Aortic Valve / Q000166:cytology; D001705:Bioprosthesis; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D006041:Goats; D006350:Heart Valve Prosthesis; D019918:Heart Valve Prosthesis Implantation; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011092:Polyethylene Glycols; D013552:Swine; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D011092:Polyethylene Glycols