title: IgE response to staphylococcal enterotoxins in adenoid tissues from atopic children.
abstract: OBJECTIVE
  To evaluate local production of staphylococcal superantigen (SAg)-specific IgE in adenoid tissue and to compare its prevalence with that in the tonsil and serum, as well as its relationship with markers of allergic inflammation within adenoid tissue.
  
  
  METHODS
  Prospective randomized study.
  
  
  METHODS
  We recruited 18 atopic children who had rhinitis symptoms and were sensitized to more than one common aeroallergen, and 22 nonatopic children undergoing adenotonsillectomy. Immunoassays were performed using adenoid tissue homogenate to quantify the levels of three staphylococcal SAg-specific IgE (SEA, SEB, and TSST-1), total IgE, eosinophil cationic protein (ECP), mast cell tryptase, and soluble CD23.
  
  
  RESULTS
  Three kinds of SAg-specific IgE were detected in the adenoid and tonsil tissues of atopic patients, but not in those of nonatopic patients. In atopic children, the prevalence of SEA-, SEB-, and TSST-1-specific IgE in adenoid tissues (61.1%, 27.8%, 33.3%, respectively) were higher than those in tonsil tissues (38.9%, 5.6%, 11.1%, respectively) and in sera (11.1%, 27.8%, 16.7%, respectively). Subjects with high SEA levels showed significantly higher serum and adenoid total IgE, with higher eosinophilia. Significant correlations were noted between SAg-specific IgE levels and tryptase levels in adenoid tissue.
  
  
  CONCLUSIONS
  Local specific IgE response to staphylococcal SAgs, especially SEA may contribute to ongoing allergic inflammation in adenoid tissue from atopic children.
authors.affiliation: Department of Otolaryngology, Kyunghee University Hospital and Kyunghee University College of Medicine, Seoul, Korea.
authors.forename: Seung-Youp
authors.initials: SY
authors.lastname: Shin
authors.forename: Gil-Soon
authors.initials: GS
authors.lastname: Choi
authors.forename: Kun-Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Sung-Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Joong-Saeng
authors.initials: JS
authors.lastname: Cho
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
mesh_terms: D000234:Adenoids / Q000276:immunology*; D000293:Adolescent; D001427:Bacterial Toxins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004768:Enterotoxins / Q000276:immunology*; D004802:Eosinophilia / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D014066:Palatine Tonsil / Q000276:immunology; D011446:Prospective Studies; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; D007073:Immunoglobulin E