Toward a PCR-independent molecular diagnosis of veterinary and medically relevant pathogenic organisms.
Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.
Partington
Matthew W
MW
School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, United Kingdom. Matthew.Partington@ncl.ac.uk
Masic
Dino
D
Nicholson
Audrey
A
Perry
John
J
Berrington
Andrew
A
West
Jonatan
J
Sparagano
Olivier A E
OA
D000818:Animals; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000662:veterinary