Toll-like receptors-related genes in kidney transplant patients with chronic allograft nephropathy and acute rejection.
BACKGROUND
Toll-like receptors (TLR) comprehend an emerging family of receptors that recognize pathogen-associated molecular patterns and promote the activation of leukocytes. Surgical trauma and ischemia-reperfusion injury are likely to provide exposure to endogenous ligands for TLR in virtually all kidney transplant recipients.


METHODS
Macroarray (GEArray OHS-018.2 Series-Superarray) analyses of 128 genes involved in TLR signaling pathway were performed in nephrectomy samples of patients with chronic allograft nephropathy (CAN) and acute rejection (AR, vascular and non vascular). The analysis of each membrane was performed by GEArray Expression Analysis Suite 2.0.


RESULTS
Macroarray profile identified a gene expression signature that could discriminate CAN and AR. Three genes were significantly expressed between CAN and vascular AR: Pellino 2; IL 8 and UBE2V1. In relation to vascular and non-vascular AR, there were only two genes with statistical significance: IL-6 and IRAK-3.


CONCLUSIONS
Vascular and non-vascular AR and CAN showed different expression of a few genes in TLR pathway. The analysis of nephrectomy showed that activation of TLR pathway is present in AR and CAN.
Nogueira
Eliana
E
Laboratory of Clinical and Experimental Immunology - Nephrology Division, Federal University of São Paulo (UNIFESP), Brazil.
Ponciano
Viviane Campos
VC
Naka
Erika L
EL
Marques
Geórgia D M
GD
Cenedeze
Marcos A
MA
Câmara
Niels Olsen Saraiva
NO
Pacheco-Silva
Alvaro
A
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002908:Chronic Disease; D005260:Female; D006084:Graft Rejection / Q000235:genetics*; D006801:Humans; D053592:Interleukin-1 Receptor-Associated Kinases / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007668:Kidney / Q000276:immunology*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000235:genetics*; D055815:Young Adult
C508600:IL6 protein, human; D015850:Interleukin-6; D051193:Toll-Like Receptors; C507771:IRAK3 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases