In vivo hemostatic effect of the medicinal plant extract Ankaferd  Blood Stopper in rats pretreated with warfarin.
OBJECTIVE
Ankaferd comprises a mixture of Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum and Urtica dioica. Ankaferd Blood Stopper (ABS) has been approved in the management of bleedings. This study aimed to evaluate in vivo hemostatic effect of ABS in rats pretreated with warfarin.


METHODS
Wistar rats (210-270 g) were treated either with warfarin (2 mg/kg) or vehicle (0.9% NaCl) orally before bilateral hind leg amputation. ABS was administered topically to one of the amputed legs. The duration of bleeding and the amount of bleeding were measured to evaluate the hemostatic effect of ABS.


RESULTS
Topical ABS administration to amputed leg shortened the duration of bleeding markedly in both untreated and warfarin-treated rats by 31.9% [1.42 min (95% CI: 0.35-2.49)] and 43.5% [5.12 min (95% CI: 2.16-8.07)] respectively. The amount of bleeding in ABS-administered amputed leg showed a decrease by 53.8% in warfarin-treated group.


CONCLUSIONS
ABS has in vivo hemostatic actions that may provide a therapeutic potential for the management of patients with deficient primary hemostasis in clinical medicine.
Cipil
Handan S
HS
Department of Hematology, Faculty of Medicine, Faith University, Ankara/Turkey.
Kosar
Ali
A
Kaya
Arif
A
Uz
Burak
B
Haznedaroglu
Ibrahim C
IC
Goker
Hakan
H
Ozdemir
Oktay
O
Koroglu
Mustafa
M
Kirazli
Serafettin
S
Firat
Huseyin Cahit
HC
D000287:Administration, Topical; D000671:Amputation, Surgical; D000818:Animals; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D006470:Hemorrhage / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D006487:Hemostasis / Q000187:drug effects*; D006490:Hemostatics / Q000008:administration & dosage / Q000494:pharmacology*; D006614:Hindlimb / Q000601:surgery; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014859:Warfarin
D006490:Hemostatics; D010936:Plant Extracts; C539751:ankaferd blood stopper; D014859:Warfarin