title: Amyloid-like properties of Saccharomyces cerevisiae cell wall glucantransferase Bgl2p: prediction and experimental evidences.
abstract: Glucantransferase Bgl2p is a major conserved cell wall constituent described for a wide range of yeast species. In the baker's yeast Saccharomyces cerevisiae it is the only non-covalently bound cell wall protein that cannot be released from cell walls by sequential SDS and trypsin treatment. It contains seven amyloidogenic determinants. Circular dichroism analysis and fluorescence spectroscopy with thioflavin T indicate the presence of beta-sheet structures in Bgl2p isolates. Bgl2p forms fibrils, a process that is enforced in the presence of other cell wall components. Thus the data obtained is the first evidence for amyloid-like properties of yeast cell wall protein-glucantransferase Bgl2p.
authors.affiliation: Department of Molecular Biology, Faculty of Biology, Lomonosov Moscow State University, Leninskie gory, Moscow, Russia. kalebina@genebee.msu.ru
authors.forename: Tatyana S
authors.initials: TS
authors.lastname: Kalebina
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Plotnikova
authors.forename: Anton A
authors.initials: AA
authors.lastname: Gorkovskii
authors.forename: Irina O
authors.initials: IO
authors.lastname: Selyakh
authors.forename: Oxana V
authors.initials: OV
authors.lastname: Galzitskaya
authors.forename: Evgeniy E
authors.initials: EE
authors.lastname: Bezsonov
authors.forename: Gerd
authors.initials: G
authors.lastname: Gellissen
authors.forename: Igor S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D002473:Cell Wall / Q000201:enzymology*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D000682:Amyloid; D029701:Saccharomyces cerevisiae Proteins; C080389:BGL2 protein, S cerevisiae; D004693:Glucan Endo-1,3-beta-D-Glucosidase