Semi-thin sections of epoxy resin-embedded mouse embryos in morphological analysis of whole mount in situ RNA hybridization.
Descriptive morphological studies are often combined with gene expression pattern analyses. Unembedded vibratome or cryotome sections are compatible with in situ RNA hybridization, but spatial resolution is rather low for precise microscopic studies necessary in embryology. Therefore, use of plastic embedding media, which allow semi-thin and ultra-thin sectioning for light and electron microscopy, could be an important advantage. This work suggested a new approach based on the whole mount hybridization of mouse embryos and subsequent epoxy resin embedding. Epoxy resin allowed serial sectioning of semi-thin sections with preserved in situ RNA hybridization signal, which was a necessary prerequisite for precise morphological analysis of embryo development.
Mitrecić
D
D
Croatian Institute for Brain Research, School of Medicine, Salata 12, HR-10000 Zagreb, Croatia. dominic@mef.hr
Cunko
V F
VF
Gajović
S
S
D000818:Animals; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000502:physiology*; D047108:Embryonic Development; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008854:Microscopy, Electron; D008867:Microtomy / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*