Sense and nonsense of high-temperature liquid chromatography.
High-temperature liquid chromatography (HTLC) is recognized today as a valuable technique in reversed-phase high-performance liquid chromatography (RP-HPLC). Column temperature can play a role in reducing analysis time, modifying retention, controlling selectivity, changing efficiency or improving detection sensitivity. The different effects of high temperatures on reversed-phase separations, the practical limitations due to the instrumentation, the limits and the main advantages of HTLC, especially for the separation of polar and ionized compounds, are reviewed.
Heinisch
Sabine
S
Laboratoire des Sciences Analytiques, UMR CNRS 5180, Université Claude Bernard Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. heinisch@univ-lyon1.fr
Rocca
Jean-Louis
JL
D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D013997:Time Factors