title: Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola.
abstract: The biosynthetic gene cluster of brassicicene C was identified in Alternaria brassicicola strain ATCC 96836 from genome database search. In vivo and in vitro study clearly revealed the function of Orf8 and Orf6 as a fusicoccadiene synthase and methyltransferase, respectively. The understanding toward the biosynthetic pathway promises construction of this type of diterpene compounds with genetic engineering.
authors.affiliation: Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan. aminami@mail.sci.hokudai.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Minami
authors.forename: Naoto
authors.initials: N
authors.lastname: Tajima
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Toyomasu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sassa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kato
authors.forename: Tohru
authors.initials: T
authors.lastname: Dairi
mesh_terms: D000528:Alternaria / Q000235:genetics*; D002474:Cell-Free System; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004224:Diterpenes / Q000737:chemistry*; D015195:Drug Design; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D016678:Genome; D008780:Methyltransferases / Q000235:genetics; D008956:Models, Chemical; D008957:Models, Genetic; D005810:Multigene Family; D010936:Plant Extracts / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004224:Diterpenes; D010936:Plant Extracts; C539991:brassicicene C; D008780:Methyltransferases