Synthesis and evaluation of photoreactive probes to elucidate iodide efflux in thyrocytes.
Four photoreactive analogues of 3-biphenyl-4'-yl-5,6-dihydroimidazo[2,1-b]thiazole were prepared and evaluated as iodide sequestering agents in sodium iodide symporter-expressing cells. One of these new photoactivatable compounds retained biological activity and was further radiolabeled with tritium. This compound may provide a useful tool for labeling, purification, and identification of target protein responsible for iodide efflux in thyrocytes.
Derbré
Séverine
S
Commissariat à l'Energie Atomique, Institut de Biologie et Technologies de Saclay (iBiTecS), Service de chimie bioorganique et de marquage, Gif-sur-Yvette F-91191, France.
Lecat-Guillet
Nathalie
N
Pillon
Florence
F
Ambroise
Yves
Y
D000818:Animals; D000838:Anions; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D007454:Iodides / Q000737:chemistry / Q000493:pharmacokinetics*; D008027:Light; D008954:Models, Biological; D008956:Models, Chemical; D010777:Photochemistry / Q000379:methods; D051381:Rats; D027981:Symporters / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry*; D013961:Thyroid Gland / Q000378:metabolism*; D014316:Tritium / Q000737:chemistry*; D000099209:Sodium-Iodide Symporters
D000838:Anions; D007454:Iodides; D027981:Symporters; D013844:Thiazoles; D014316:Tritium; D000099209:Sodium-Iodide Symporters