title: [Polymorphism analysis of human cytomegalovirus UL150 gene in low passage clinical isolates].
abstract: OBJECTIVE
  To investigate the polymorphism of human cytomegalovirus UL150 gene in low passage clinical isolates and try to study the relationship between the polymorphism and different pathogenesis of congenital HCMV infection.
  
  
  METHODS
  PCR was performed to amplify the entire HCMV UL150 gene region of 29 clinical isolates, which had been proven containing detectable HCMV-DNA by using FQ-PCR. PCR amplification products were sequenced directly and the data were analysed.
  
  
  RESULTS
  25 among 29 isolates were amplified and 18 isolates were sequenced successfully. By comparison with the sequence of Toledo and Merlin, the length of UL150 ORFs in all 18 clinical isolates was similar to that of Merlin than Toledo.
  
  
  CONCLUSIONS
  HCMV UL150 DNA and deduced amino acid sequences is hypervariability.
authors.affiliation: The Shengjing Hospital, Affiliated to China Medical University, Shen yang 110004, China. jiyh@cmu2h.com
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Ji
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Ma
authors.forename: Zheng-Rong
authors.initials: ZR
authors.lastname: Sun
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003587:Cytomegalovirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014764:Viral Proteins