Lactoferrin suppress the adipogenic differentiation of MC3T3-G2/PA6 cells.
Lactoferrin accelerates the differentiation of osteogenic and chondrogenic lineage cells, whereas it inhibits the myogenic and adipogenic differentiation of pluripotent mesenchymal cells; however, the effect of lactoferrin on the differentiation of preadipocytes is unknown. In this study, we examined the effect of lactoferrin on adipogenic differentiation using a mouse preadipocyte cell line, MC3T3-G2/PA6. The cells were cultured in differentiation medium with or without lactoferrin to induce cellular differentiation. The cell lineage was then determined by Oil Red O staining, real-time PCR screening for the mRNA expression of phenotype-specific markers, and Western blot analysis. The number of Oil Red O-positive lipid droplets decreased following treatment with lactoferrin, as did the mRNA expression of C/EBPalpha, PPARgamma, aP2, and adiponectin. Furthermore, our Western blot data revealed a decrease in PPARgamma expression attributable to lactoferrin exposure. These results suggest that lactoferrin suppresses the adipogenic differentiation of MC3T3-G2/PA6 cells.
Yagi
Motohiko
M
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
Suzuki
Naoto
N
Takayama
Tadahiro
T
Arisue
Masatoshi
M
Kodama
Takuya
T
Yoda
Yasushi
Y
Numasaki
Hikaru
H
Otsuka
Kichibee
K
Ito
Koichi
K
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000032:analysis; D000818:Animals; D001391:Azo Compounds; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D050556:Fatty Acid-Binding Proteins / Q000032:analysis; D007781:Lactoferrin / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D051379:Mice; D047495:PPAR gamma / Q000032:analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
D052242:Adiponectin; C499919:Adipoq protein, mouse; D001391:Azo Compounds; D015415:Biomarkers; D022763:CCAAT-Enhancer-Binding Protein-alpha; D004396:Coloring Agents; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D008055:Lipids; D047495:PPAR gamma; D012333:RNA, Messenger; D007781:Lactoferrin; C011049:oil red O