Effects of zidovudine and stavudine on mitochondrial DNA of differentiating 3T3-F442a cells are not associated with imbalanced deoxynucleotide pools.
To test whether zidovudine (3'-azido-3'-deoxythymidine) (AZT) inhibition of thymidine phosphorylation causes depletion of the TTP pool resulting in mitochondrial DNA depletion, 3T3-F442a cells were differentiated in the presence of AZT and analyzed to determine mitochondrial DNA content and deoxynucleotide levels. These results suggest that AZT toxicity may not be related to deoxynucleotide pool alterations.
Lynx
Matthew D
MD
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA.
LaClair
Darcy D
DD
McKee
Edward E
EE
D016475:3T3 Cells; D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D051379:Mice; D018119:Stavudine / Q000494:pharmacology*; D015215:Zidovudine / Q000494:pharmacology*
D019380:Anti-HIV Agents; D004272:DNA, Mitochondrial; D003854:Deoxyribonucleotides; D015215:Zidovudine; D018119:Stavudine