[RNA interference targeting aquaporin-1 on inhibition of human vascular endothelial cell migration in vitro].
OBJECTIVE
To investigate inhibition of human vascular endothelial cell migration in vitro by RNA interference targeting AQP-1, and the mechanism of corneal neovascularization.


METHODS
Experiment research. Human umbilical vein endothelial cell (HUVEC) was cultured, AQP-1 specific siRNA was designed and synthesized. siRNA was transfected into the HUVEC by Lipofectamine 2000. The mRNA of AQP-1 was assessed by reverse transcription-polymerase chain reaction (RT-PCR). Transwell migration assay and wound healing assay were used to detect the migration capability of HUVEC. The group comparison of AQP-1 mRNA expression was used one-way ANOVA test The result of transwell migration assay and wound healing assay was analysed with Independent-Sample T test.


RESULTS
The expression of AQP-1 mRNA was significantly (P < or = 0.05) suppressed after transfection. 24 h, 48 h and 72 h, after transfection, the expression of AQP-1 mRNA was 11.3%, 17.4% and 29.7% respectively compared to normal control (P = 0.004, 0.007, 0.002). Transwell migration assay and wound healing assay showed that the migration capability of HUVEC was decreased obviously (t = 10.813, P = 0.016 and t = 22.431, P = 0.027).


CONCLUSIONS
AQP-1 specific siRNA is effective in suppressing the AQP-1 expression and inhibiting the migration of the cultured HUVEC. Reducing the expression of AQP-1 can inhibit the corneal neovascularization.
Zhang
Hong-xu
HX
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zhang
Ming-chang
MC
Chen
Hong
H
D051398:Aquaporin 1 / Q000235:genetics*; D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034741:RNA, Small Interfering; D051398:Aquaporin 1