Effects of cytotoxins on intracellular na/K balance in mouse embryonic cell.
Cytoplasmic Na/K imbalance induced by cytotoxic substances (ethylene glycol and cytochalasin B) was studied. Incubation in Dulbecco's medium with these cytotoxins caused changes in K and Na concentrations in the mouse two-cell embryo blastomer. The effects of both substances resulted in a drop of Na content in the embryonic cell. Washing from cytochalasin B restored the initial Na/K balance in the cytoplasm. Possible adaptive mechanisms involved in the regulation of intracellular ionic homeostasis are discussed.
Pogorelov
A G
AG
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Russia. agpogorelov@rambler.ru
Goldshtein
D V
DV
Pogorelova
V N
VN
D000465:Algorithms; D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology; D003603:Cytotoxins / Q000494:pharmacology*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D019855:Ethylene Glycol / Q000494:pharmacology; D006706:Homeostasis / Q000187:drug effects*; D007424:Intracellular Fluid / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011188:Potassium / Q000032:analysis* / Q000378:metabolism; D012964:Sodium / Q000032:analysis* / Q000378:metabolism
D003603:Cytotoxins; D003571:Cytochalasin B; D012964:Sodium; D019855:Ethylene Glycol; D011188:Potassium