Targeted disruption of the mouse Lipoma Preferred Partner gene.
LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp(-/-) females. Fertility of Lpp(-/-) males was proven to be normal, however, females from Lpp(-/-) x Lpp(-/-) crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp(-/-) mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp(-/-) mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter.
Vervenne
Hilke B V K
HB
Laboratory for Molecular Oncology, Department of Human Genetics, Herestraat 49, Box 602, K.U. Leuven, Leuven, Belgium.
Crombez
Koen R M O
KR
Delvaux
Els L
EL
Janssens
Veerle
V
Van de Ven
Wim J M
WJ
Petit
Marleen M R
MM
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020964:Embryo Loss / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D018390:Gene Targeting; D060588:LIM Domain Proteins; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000235:genetics; D012098:Reproduction / Q000235:genetics; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic
D003598:Cytoskeletal Proteins; D060588:LIM Domain Proteins; C491227:Lpp protein, mouse