Assessment of the extent of starch dissolution in dimethyl sulfoxide by 1H NMR spectroscopy.
Complete dissolution is needed for the separation, characterization, or homogeneous labeling of whole starch molecules. A method is presented to quantify the extent of starch dissolution in DMSO for the first time; it is validated on a commercial rice starch. It is used directly on starch dispersions containing possible undissolved or co-dissolved species. High-amylose maize starches, known to be digested slowly in vivo, only quantitatively dissolve in the presence of high concentrations of an H-bond disrupter, LiBr, although they form clear dispersions at low LiBr concentrations. Starch quantitatively dissolves from waxy rice flours; non-starch components partially co-dissolve but do not interfere with the dissolution quantification.
Schmitz
Sarah
S
The University of Queensland, Centre for Nutrition and Food Sciences, Brisbane, QLD, Australia.
Dona
Anthony C
AC
Castignolles
Patrice
P
Gilbert
Robert G
RG
Gaborieau
Marianne
M
D001965:Bromides / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D006801:Humans; D018020:Lithium Compounds / Q000737:chemistry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012275:Oryza / Q000737:chemistry; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry
D001965:Bromides; D018020:Lithium Compounds; D012997:Solvents; C040949:lithium bromide; D013213:Starch; D004121:Dimethyl Sulfoxide