Monitoring patterns of gene expression in Dictyostelium by beta-galacotsidase staining.
Monitoring the spatial distribution of prespore and pstB cell types is a sensitive method to monitor GSK-3 and Aar activity during Dictyostelium development. Cell-specific expression of lacZ marker genes can be readily detected using enzymatic cleavage of the substrate X-gal. This protocol describes a simple method for beta-galactosidase staining in developed Dictyostelium structures.
Harwood
Adrian J
AJ
Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK.
D000818:Animals; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D001616:beta-Galactosidase / Q000378:metabolism*
D001616:beta-Galactosidase