title: Echinococcus granulosus: protoscolicidal effect of high intensity focused ultrasound.
abstract: High intensity focused ultrasound (HIFU) is a new non-invasive technique which can cause cell death and tissue necrosis by focusing high-energy ultrasonic waves on a single location. The aim of our work is to investigate the damaging effect of HIFU on Echinococcus granulosus protoscolices, as well as its inhibitory effect on growth of hydatid cysts derived from protoscolices. The damaging effect of HIFU on protoscolices was investigated by following parasite mortality after irradiation, while the inhibitory effect was investigated by infection experiments in vivo. The results demonstrated that HIFU was able to damage protoscolices and the protoscolicidal effect was dose-dependent and showed late-onset. The growth of protoscolices that survived the exposure to HIFU was obviously suppressed in vitro, and the mean weight of hydatid cysts resulting from such protoscolices in the experimental group was less than that in controls. Evidences including the protoscolicidal effect, fragmentized protoscolices and low post exposure temperatures, suggest that cavitation may contribute to the protoscolicidal effect of HIFU. In addition, the structure of the germinal membrane in cysts developing from the irradiated protoscolices was not as normal or intact as that from non-irradiated ones, and morphological changes related to degeneration were observed, suggesting that HIFU could prevent protoscolices from developing normal germinal membrane and consequently stop the proliferation of secondary hydatid cysts. HIFU demonstrated damaging effect on protoscolices, inhibited the growth of protoscolices in vitro and in vivo, and could be a possible therapeutic option for cystic echinococcosis.
authors.affiliation: Department of Pathogenetic Biology, Chongqing Medical University, 1# Medical College Road, Chongqing 400016, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Zou
authors.forename: Junan
authors.initials: J
authors.lastname: Wang
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Weihua
authors.initials: W
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
mesh_terms: D000818:Animals; D004443:Echinococcosis / Q000628:therapy; D048209:Echinococcus granulosus / Q000254:growth & development / Q000472:pathogenicity; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D012756:Sheep; D013696:Temperature; D014464:Ultrasonic Therapy; D014465:Ultrasonics / Q000145:classification