title: Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography as an efficient and sensitive technique for simultaneous determination of chloramphenicol and thiamphenicol in honey.
abstract: Dispersive liquid-liquid microextraction (DLLME) coupled with high-performance liquid chromatography-variable wavelength detector (HPLC-VWD) was developed for extraction and determination of chloramphenicol (CAP) and thiamphenicol (THA) in honey. In this extraction method, 1.0 mL of acetonitrile (as dispersive solvent) containing 30 microL 1,1,2,2-tetrachloroethane (as extraction solution) was rapidly injected by syringe into a 5.00-mL water sample containing the analytes, thereby forming a cloudy solution. After extraction, phase separation was performed by centrifugation and the enriched analytes in the sedimented phase were determined by HPLC-VWD. Some important parameters, such as the nature and volume of extraction solvent and dispersive solvent, extraction time, sample solution pH, sample volume and salt effect were investigated and optimized. Under the optimum extraction condition, the method yields a linear calibration curve in the concentration range from 3 to 2000 microg kg(-1) for target analytes. The enrichment factors for CAP and THA were 68.2 and 87.9, and the limits of detection (S/N=3) were 0.6 and 0.1 microg kg(-1), respectively. The relative standard deviations (RSDs) for the extraction of 10 microg kg(-1) of CAP and THA were 4.3% and 6.2% (n=6). The main advantages of DLLME-HPLC method are simplicity of operation, rapidity, low cost, high enrichment factor, high recovery, good repeatability and extraction solvent volume at microliter level. Honey samples were successfully analyzed using the proposed method.
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. hxch@hubu.edu.cn
authors.forename: Huaixia
authors.initials: H
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.forename: Jianlin
authors.initials: J
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Liao
mesh_terms: D002701:Chloramphenicol / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D006722:Honey / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D012997:Solvents; D013839:Thiamphenicol / Q000032:analysis* / Q000737:chemistry*; D013997:Time Factors
chemical_list: D012997:Solvents; D002701:Chloramphenicol; D013839:Thiamphenicol