[Effect of zhenggu powder ([Chinese characters: see text]) on the inflammatory cell factor of soft tissue in experimental acute injury].
OBJECTIVE
To probe the recovery mechanism of Zhenggu powder (ZGP) on acute soft tissue injury in cell levels.


METHODS
Forty rabbits which established animal model of acute soft tissue injury with hammer hitting,were divided randomly into normal group (A), model group (B), vaseline group (C)and ZGP group (D). Injured part was applied external drug daily after model was made. All animals were killed after using drug for 4 days. The local tissue of injured part was taken pathologic study, and was measured the content of IL-1beta, IL-6, TNFalpha by ELISA method and TXB2, 6-keto-PGF1alpha by RIA method.


RESULTS
Muscular tissue of group A was normal. But that of group B and C was aberrant,such as swelling and broken of muscular fiber or infiltration of inflammatory cell. Such histological change of group D was lightly and hyperplasia of blood vessel was found. The contents of IL-1beta, TNFalpha, TXB2 and TXB2/6-keto-PGF1alpha in group D were lower than that of group B and group C. On the contrary, the contents of 6-keto-PGF1alpha in the group D were higher than that of group B and group C. The difference of content of IL-6 between groups was not obvious.


CONCLUSIONS
ZGP could promote not only the dilution and the transportation of inflammatory cell factors,but also the repair and the regeneration of the injured tissue structures. The therapeutic effect of ZGP was not relative to IL-6.
Qi
Yue-feng
YF
Fengsheng Orthopaedics and Traumatology Hospital of TCM of Beijing, Beijing 100034, China.
Lu
Jian-qiu
JQ
Zhao
Xing-wei
XW
Han
Jing
J
D000818:Animals; D016207:Cytokines / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D017695:Soft Tissue Injuries / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators