The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation.
OBJECTIVE
To measure serum cholinesterase (SCHE) with an integration strategy.


METHODS
At 54.0 micromol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 micromol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 60%, or were determined by the classical initial rate method.


RESULTS
The linear range was from 16 to 1560 nkat/L, and SCHE in clinic sera showed negligible substrate-activation.


CONCLUSIONS
This strategy was effective.
Liao
Fei
F
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing 400016, China. liaofeish@yahoo.com
Yang
Diyuan
D
Tang
Jingqun
J
Yang
Xiaolan
X
Liu
Beizhong
B
Zhao
Yunsheng
Y
Zhao
Lina
L
Liao
Hong
H
Yu
Ming'an
M
D001681:Biological Assay / Q000379:methods*; D002802:Cholinesterases / Q000097:blood*; D006801:Humans
D002802:Cholinesterases