[The effects of extracts from Arenaria kansuensis on the levels of HBsAG and HBeAg in HepG2.2.15 cells].
OBJECTIVE
To investigate the anti-HBV effects of the extracts with alcohol and water from Arenaria kansuensis in vitro.


METHODS
HepG2.2.15 cells were used to estimate the effects of the extracts with alcohol and water from Arenaria kansuensis on the levels of HBsAg and HBeAg in medium by ELISA.


RESULTS
Incubation of HepG2.2.15 cells with both the extracts with alcohol and water from Arenaria kansuensis (50-400 mg/L) for 72 and 96h significantly inhibited the levels of HBsAg and HBeAg. The maximum inhibiting rate of HBeAg from the Arenaria kansuensis extracts with alcohol was 63.2% and 90. 8% at 72h and 96h, respectively. Meanwhile, the Arenaria kansuensis extracts with water had obvious inhibitory effects on the levels of HBsAg and HBeAg in HepG2.2.15 cells at 96 h, compared with that of the control group (P < 0.01). The maximum inhibiting rates were 52.5% and 72.8%, respectively.


CONCLUSIONS
The extracts with water from Arenaria kansuensis has obvious anti-HBV effects in vitro.
Tang
Qing-nian
QN
The 181st hospital of P. L. A, Guilin 541002, China.
Feng
Mei
M
Gong
Shou-ji
SJ
Dai
Zhi-kai
ZK
Zhou
Yi-zhi
YZ
Xv
Qing
Q
D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D029748:Caryophyllaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D010936:Plant Extracts; D014867:Water; D000431:Ethanol