title: Evaluation of the number of ionogenic groups of inulinase by acid-base titration.
abstract: Acid base titration showed that Aspergillus awamori inulinase includes 178 asparaginic and glutamic acid residues, 20 histidine, 10 serine, and 34 lysine and tyrosine residues. Denaturation temperature for this enzyme was calculated using analysis of the proportion of stabilizing and destabilizing amino acids in the molecule.
authors.affiliation: Voronezh State University. tamara_kovaleva@inbox.ru
authors.forename: T A
authors.initials: TA
authors.lastname: Kovaleva
authors.forename: M G
authors.initials: MG
authors.lastname: Holyavka
authors.forename: S G
authors.initials: SG
authors.lastname: Rezvan
authors.forename: S V
authors.initials: SV
authors.lastname: Kozhedub
mesh_terms: D001216:Asparagine / Q000737:chemistry; D001230:Aspergillus / Q000201:enzymology*; D005656:Fungal Proteins / Q000737:chemistry*; D018698:Glutamic Acid / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry; D011489:Protein Denaturation; D012694:Serine / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D018698:Glutamic Acid; D014443:Tyrosine; D012694:Serine; D006639:Histidine; D001216:Asparagine; D006026:Glycoside Hydrolases; C023086:inulinase; D008239:Lysine