The allergic inflammatory reaction in neonatal streptozotocininduced diabetic rats: evidence of insulin resistance and microvascular dysfunction.
OBJECTIVE
To investigate the allergic reaction in neonatal streptozotocin (nSTZ)-induced diabetes mellitus.


METHODS
Male newborn Wistar rats were made diabetic by the injection of streptozotocin (160 mg/kg, i. p.) and used 8 weeks thereafter.


METHODS
Animals were sensitized against ovalbumin (OA, 50 microg and Al(OH)3, 5 mg, s. c.) and challenged 14 or 21 days thereafter.


METHODS
OA-induced airway inflammation and OA-induced pleurisy models were used to investigate leukocyte migration (total and differential leukocyte counts) and lung vascular permeability (Evans blue dye extravasation).


RESULTS
nSTZ-diabetic rats presented glucose intolerance and insulin resistance. Relative to controls, nSTZ rats exhibited a 30% to 50% reduction in lung vascular permeability. Leukocyte infiltration in both models of allergen-induced inflammation, and number of pleural mast cells did not differ between groups.


CONCLUSIONS
Data suggest that the reduction of allergic inflammatory reactions in nSTZ rats is restricted to microvascular dysfunctions and associated, probably, with insulin resistance in lung microvascular endothelium.
Cavalher-Machado
S C
SC
Research Division, Institute of Heart (InCor), LIM-11, University of São Paulo Medical School, São Paulo, SP, Brazil. simachado@far.fiocruz.br
Cuman
R K N
RK
Sartoretto
J L
JL
Martins
J O
JO
de Lima
W Tavares
WT
Martins
M A
MA
Silva
P M R
PM
Sannomiya
P
P
D000818:Animals; D000831:Animals, Newborn; D002199:Capillary Permeability; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005951:Glucose Tolerance Test; D006967:Hypersensitivity / Q000209:etiology*; D007249:Inflammation / Q000209:etiology*; D007333:Insulin Resistance; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010998:Pleurisy / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D013311:Streptozocin; D010047:Ovalbumin