title: Characterization of beta-hemolytic streptococci isolated from southern sea otters (Enhydra lutris nereis) stranded along the California coast.
abstract: The goal of this study was to characterize the beta-hemolytic streptococci cultured from southern sea otters (Enhydra lutris nereis) stranded off the coast of California (USA) and to verify identifications made using the Lancefield system. Lancefield serotyping and biochemical analysis alone was inadequate for isolate characterization. Final identification was based on sequence analysis of a portion of the 16s ribosomal RNA gene from 12 of the 35 total isolates. The majority of isolates (10 of 12; 83.3%) were Streptococcus phocae and reacted with Lancefield group G and F antisera or were less frequently untypeable. The remaining isolates belonging to Lancefield group G were identified as S. dysgalactiae subsp. equisimilis (2 of 12; 16.7%). This is the first report of S. phocae in southern sea otters and further evidence of S. phocae expressing cell surface antigens compatible with Lancefield group G typing.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, CA 95616, USA. dmimai@ucdavis.edu
authors.forename: Denise
authors.initials: D
authors.lastname: Imai
authors.forename: Spencer
authors.initials: S
authors.lastname: Jang
authors.forename: Melissa
authors.initials: M
authors.lastname: Miller
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Conrad
mesh_terms: D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010041:Otters / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D012703:Serotyping / Q000662:veterinary; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S