Platelet P2Y(12) receptor influences the vessel wall response to arterial injury and thrombosis.
BACKGROUND
Platelets are believed to play an important role in atherogenesis and the vessel response to vascular injury. The P2Y(12) receptor (P2Y(12)) plays a central role in amplifying platelet aggregation, dense granule and alpha-granule secretion, P-selectin expression, microparticle formation, and procoagulant membrane changes, regardless of the activating stimulus. We hypothesized that P2Y(12) deficiency might reduce the vessel wall response to vascular injury as well as thrombosis in murine vascular injury models.


RESULTS
P2Y(12)-deficient (-/-) mice and littermate controls (+/+) were bred on a C57 BL/6 background. In vivo murine models of arterial injury were employed alone and in combination with bone marrow transplantation to investigate the role of P2Y(12) in the vessel wall response to arterial injury and thrombosis. At 21 days after ferric chloride injury, neointima formation in P2Y(12)(-/-) arteries was significantly less than that observed in control strain arteries (P<0.025). In agreement with this, the intima-media ratio was significantly greater in femoral wire-injured arteries from P2Y(12)(+/+) compared with P2Y(12)(-/-) animals (P<0.05). Bone marrow transplantation was used to examine the importance of vessel wall P2Y(12) versus platelet P2Y(12). Analysis of arterial sections from chimeric animals at 21 days after injury revealed a smaller intima-media ratio in -/- to +/+ animals than in the positive (+/+ to +/+) control group (P<0.01).


CONCLUSIONS
These data demonstrate a role for platelet P2Y(12) in the vessel wall response to arterial injury and thrombosis. This illustrates the manner in which platelets may contribute to atherogenesis and restenosis.
Evans
D J W
DJ
Cardiovascular Research Unit, School of Medicine and Biomedical Sciences, Sheffield, UK.
Jackman
L E
LE
Chamberlain
J
J
Crosdale
D J
DJ
Judge
H M
HM
Jetha
K
K
Norman
K E
KE
Francis
S E
SE
Storey
R F
RF
D000818:Animals; D050197:Atherosclerosis / Q000473:pathology / Q000503:physiopathology; D001792:Blood Platelets / Q000473:pathology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002712:Chlorides; D004195:Disease Models, Animal; D005260:Female; D005263:Femoral Artery / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D005290:Ferric Compounds / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009131:Muscle, Smooth, Vascular / Q000473:pathology / Q000503:physiopathology; D009676:Noxae / Q000633:toxicity; D019007:P-Selectin / Q000378:metabolism; D010974:Platelet Aggregation / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000235:genetics* / Q000378:metabolism*; D058925:Receptors, Purinergic P2Y12; D013927:Thrombosis / Q000473:pathology / Q000503:physiopathology*; D017539:Tunica Intima / Q000293:injuries / Q000473:pathology / Q000503:physiopathology
D002712:Chlorides; D005290:Ferric Compounds; D009676:Noxae; D019007:P-Selectin; C548577:P2ry12 protein, mouse; D018048:Receptors, Purinergic P2; D058925:Receptors, Purinergic P2Y12; C024555:ferric chloride