title: A novel dengue vaccine candidate that induces cross-neutralizing antibodies and memory immunity.
abstract: A novel dengue vaccine candidate comprised of a consensus dengue virus envelope protein domain III (cED III) was developed to fight against dengue virus infection. The amino acid sequence of this novel cED III was obtained by alignment of amino acid sequences from different isolates of the four serotypes of dengue viruses. A proof-of-concept study demonstrated that BALB/c mice immunized with the recombinant cED III developed neutralizing antibodies against all serotypes of dengue virus. Moreover, formulation of recombinant cED III with aluminum phosphate could induce long-lasting antibody responses and anamnestic neutralizing antibody responses following challenge with dengue virus at week 28 after priming. These results demonstrate the possibility of developing a single tetravalent vaccine against dengue viral infections.
authors.affiliation: Vaccine Research and Development Center, National Health Research Institute, No. 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan.
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Leng
authors.forename: Shih-Jen
authors.initials: SJ
authors.lastname: Liu
authors.forename: Jy-Ping
authors.initials: JP
authors.lastname: Tsai
authors.forename: Yi-Shiuan
authors.initials: YS
authors.lastname: Li
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Chen
authors.forename: Hsueh-Hung
authors.initials: HH
authors.lastname: Liu
authors.forename: Shu-Pei
authors.initials: SP
authors.lastname: Lien
authors.forename: Andrew
authors.initials: A
authors.lastname: Yueh
authors.forename: Kuang-Nan
authors.initials: KN
authors.lastname: Hsiao
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Lai
authors.forename: Feng-Chuan
authors.initials: FC
authors.lastname: Liu
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
authors.forename: Hsin-Wei
authors.initials: HW
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016384:Consensus Sequence; D053059:Dengue Vaccines / Q000235:genetics / Q000276:immunology*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010710:Phosphates / Q000008:administration & dosage / Q000494:pharmacology; D016415:Sequence Alignment; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000914:Antibodies, Viral; D053059:Dengue Vaccines; C051821:E-glycoprotein, Dengue virus type 2; D010710:Phosphates; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C012714:aluminum phosphate