title: Affinity of Tomopenem (CS-023) for penicillin-binding proteins in Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.
abstract: Tomopenem (formerly CS-023), a novel 1beta-methylcarbapenem, exhibited high affinity for penicillin-binding protein (PBP) 2 in Staphylococcus aureus, PBP 2 in Escherichia coli, and PBPs 2 and 3 in Pseudomonas aeruginosa, which are considered major lethal targets. Morphologically, tomopenem induced spherical forms in E. coli and short filamentation with bulges in P. aeruginosa, which correlated with the drug's PBP profiles. The potential of resistance of these bacteria to tomopenem was comparable to that to imipenem.
authors.affiliation: Biological Research Laboratories IV, Daiichi Sankyo Co, Ltd, Tokyo, Japan. koga.tetsufumi.zj@daiichisankyo.co.jp
authors.forename: Tetsufumi
authors.initials: T
authors.lastname: Koga
authors.forename: Chika
authors.initials: C
authors.lastname: Sugihara
authors.forename: Masayo
authors.initials: M
authors.lastname: Kakuta
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Masuda
authors.forename: Eiko
authors.initials: E
authors.lastname: Namba
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukuoka
mesh_terms: D015780:Carbapenems / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D009154:Mutation; D046915:Penicillin-Binding Proteins / Q000378:metabolism* / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D015780:Carbapenems; D046915:Penicillin-Binding Proteins; C492232:tomopenem