Hemosiderin deposits confounds tracking of iron-oxide-labeled stem cells: an experimental study.
BACKGROUND
The aim of the present research was to study the possible interference of hemosiderin deposits with the histological detection of dextran-coated, iron-labeled, mesenchymal stem cells after intracoronary administration in a porcine model of myocardial infarction.


METHODS
A myocardial infarction was induced in six animals that received intracoronary iron-labeled autologous mesenchymal stem cells (group 1; n = 2) or placebo (group 2; n = 4). Six control animals without myocardial infarction underwent direct intramyocardial injections of iron-labeled autologous mesenchymal stem cells (group 3; n = 2) or placebo (group 4; n = 4). Histological sections from explanted hearts were stained with Prussian blue to identify dextran-coated, iron-labeled, mesenchymal stem cells.


RESULTS
After Prussian blue staining, granular blue labeling in the tissue was observed in both groups of animals with infarcts. Similar granular blue labeling was detected in hearts from control animals without infarction that had received iron-labeled mesenchymal stem cells. However, hearts from control animals without infarction that received placebo did not have any granular blue labeling in the tissue.


CONCLUSIONS
Hemosiderin from infarction hemorrhage interferes with detection of dextran-coated iron-labeled mesenchymal stem cells after intracoronary administration, suggesting that this marker is not useful to detect mesenchymal stem cells in a porcine model of myocardial infarction.
Rigol
M
M
Institut Clínic del Tòrax, Hospital Clínic, Barcelona, Spain. mrigol@clinic.ub.es
Solanes
N
N
Roqué
M
M
Farré
J
J
Batlle
M
M
Roura
S
S
Bellera
N
N
Prat-Vidal
C
C
Sionis
A
A
Ramírez
J
J
Sitges
M
M
Sanz
G
G
Bayés-Genís
A
A
Heras
M
M
D000818:Animals; D002469:Cell Separation / Q000379:methods; D004195:Disease Models, Animal; D005290:Ferric Compounds / Q000032:analysis; D006485:Hemosiderin / Q000032:analysis*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000473:pathology* / Q000601:surgery; D033581:Stem Cell Transplantation / Q000379:methods*; D013552:Swine; D014182:Transplantation, Autologous
D005290:Ferric Compounds; C000499:ferric oxide; D006485:Hemosiderin