Apoptosis inducement of bigelovin from Inula helianthus-aquatica on human Leukemia U937 cells.
Inula helianthus-aquatica C. Y. Wu is a traditional medicinal plant used to treat some cancers in folk herbal medicine of Yunnan, China. Bigelovin, a sesquiterpene lactone isolated from this herb, potently inhibits the growth of a panel of eight cancer cell lines, especially in human monoblastic leukemia U937 cells with an IC(50) value of 0.47 microM. Characteristic morphological features of apoptosis were observed in U937 cells treated with bigelovin. Annexin V and nuclear DNA content distribution assays showed that the percentage of Annexin V positive cells increased to 8.86% (24 h) with 1 microM bigelovin treatment, and cells treated with bigelovin at this concentration apparently arrested at G(0)/G(1) phase compared with the control. These data suggested that cytotoxic effect of bigelovin on U937 cells involves induction of apoptosis, and the cell cycle is arrested at G(0)/G(1) phase.
Zeng
Guang-Zhi
GZ
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
Tan
Ning-Hua
NH
Ji
Chang-Jiu
CJ
Fan
Jun-Ting
JT
Huang
Huo-Qiang
HQ
Han
Hong-Jin
HJ
Zhou
Guang-Biao
GB
D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D006801:Humans; D038143:Inula / Q000737:chemistry*; D007783:Lactones / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology*; D020298:U937 Cells
D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D007783:Lactones; D012717:Sesquiterpenes; C099796:bigelovin