Assaying beta-catenin/TCF transcription with beta-catenin/TCF transcription-based reporter constructs.
Transcription-based reporters have been instrumental in characterizing the Wnt/beta-catenin signaling pathway and will be essential in the search for therapeutics aimed at combating diseases linked to aberrant signaling. In this chapter, we introduce a new improved Wnt/beta-catenin reporter system, beta-catenin-activated reporter (BAR), and its accompanying control reporter system, found unresponsive BAR (fuBAR). Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field.
Biechele
Travis L
TL
Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA, USA.
Moon
Randall T
RT
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D051820:TCF Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
D051820:TCF Transcription Factors; D051176:beta Catenin