siRNA-targeted COL8A1 inhibits proliferation, reduces invasion and enhances sensitivity to D-limonence treatment in hepatocarcinoma cells.
The COL8A1 (collagen type VIII, alpha-1) gene, which encodes the alpha 1 chain of collagen, type VIII, may modulate migration, proliferation and adherence of various cells. Only very sparse information exists on COL8A1 expression in hepatocarcinoma. To investigate the possible role of COL8A1 in the mouse hepatocarcinoma cell line Hca-F with highly metastatic potential in the lymph nodes, we used an RNA interference (RNAi) approach to silence COL8A1 expression. The results showed that a small interfering RNA (siRNA) targeted against COL8A1 significantly impeded Hca-F cells proliferation and colony formation in soft agar. This reduction of COL8A1 expression also led to the decreased invasion of Hca-F cells dramatically in vitro. Furthermore, the downregulation of COL8A1 expression also sensitized cells to the action of D-limonene. These data together provide insights into the function of COL8A1 and suggest that COL8A1 might represent a new potential target for gene therapy in hepatocarcinoma.
Zhao
Yongfu
Y
Department of General Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China.
Jia
Li
L
Mao
Xiaoyan
X
Xu
Henggui
H
Wang
Bo
B
Liu
Yongji
Y
D000818:Animals; D016588:Anticarcinogenic Agents / Q000378:metabolism*; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D024102:Collagen Type VIII / Q000378:metabolism*; D053138:Cyclohexenes / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D000077222:Limonene; D008113:Liver Neoplasms / Q000378:metabolism*; D051379:Mice; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*
D016588:Anticarcinogenic Agents; D024102:Collagen Type VIII; D053138:Cyclohexenes; D017931:DNA Primers; D034741:RNA, Small Interfering; D013729:Terpenes; D000077222:Limonene