title: Methods of calculating protein hydrophobicity and their application in developing correlations to predict hydrophobic interaction chromatography retention.
abstract: Hydrophobic interaction chromatography (HIC) is a key technique for protein separation and purification. Different methodologies to estimate the hydrophobicity of a protein are reviewed, which have been related to the chromatographic behavior of proteins in HIC. These methodologies consider either knowledge of the three-dimensional structure or the amino acid composition of proteins. Despite some restrictions; they have proven to be useful in predicting protein retention time in HIC.
authors.affiliation: Department of Chemical Engineering, University of Santiago of Chile, Av. Libertador Bernardo O'Higgins 3363, Santiago, Chile. amahn_2000@yahoo.es
authors.forename: Andrea
authors.initials: A
authors.lastname: Mahn
authors.forename: M Elena
authors.initials: ME
authors.lastname: Lienqueo
authors.forename: J Cristian
authors.initials: JC
authors.lastname: Salgado
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D011506:Proteins