title: Epigenetics meets next-generation sequencing.
abstract: Next-generation sequencing is poised to unleash dramatic changes in every area of molecular biology. In the past few years, chromatin immunoprecipitation (ChIP) on tiled microarrays (ChIP-chip) has been an important tool for genome-wide mapping of DNA-binding proteins or histone modifications. Now, ChIP followed by direct sequencing of DNA fragments (ChIP-seq) offers superior data with less noise and higher resolution and is likely to replace ChIP-chip in the near future. We will describe advantages of this new technology and outline some of the issues in dealing with the data. ChIP-seq generates considerably larger quantities of data and the most challenging aspect for investigators will be computational and statistical analysis necessary to uncover biological insights hidden in the data.
authors.affiliation: Harvard Medical School Center for Biomedical Informatics, Harvard-Partners Center for Genetics and Genomics, HST Informatics Program at Children's Hospital Boston, Boston, Massachusetts 02115, USA. peter_park@harvard.edu
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D003299:Cooperative Behavior; D044127:Epigenesis, Genetic; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D009707:Nucleosomes