title: Response of the periapical tissue of dogs' teeth to the action of citric acid and EDTA.
abstract: The purpose of this study was to analyze the inflammatory response of dog's periapical tissues to 17% trisodium EDTA salt (pH 8.0) and 1% citric acid (pH 2.0). Saline was used as a control. Six adult dogs were used as the biological model of the study. The experimental units comprised 56 roots of mandibular molars (first and second) and premolars (first, second and third). After coronal opening, pulpectomy and root canal instrumentation were performed using the above-mentioned irrigating solutions. After 24 and 48 hours, the animals were euthanized and the teeth and their supporting tissues were removed and histologically processed. The sections were stained with hematoxylin and eosin and analyzed histopathologically with a light microscope at x100 magnification. The histological analysis focused on the occurrence of acute inflammatory response. The presence of swelling, vasodilatation and inflammatory cells were evaluated and the degree of inflammation was determined for each case. Data were analyzed by Fisher's exact test using the SPSS software with a confidence interval of 95% (p<0.05). 17% EDTA and 1% citric acid caused inflammatory responses in dog's periapical tissues with no significant differences to each other or to saline (control) at either the 24-hour (p=0.482) or 48-hour (p=0.377) periods. It may be concluded that the inflammatory response was of mild intensity for the tested substances.
authors.affiliation: Department of Clinics, Dental School, Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Cristina Berthold
authors.initials: CB
authors.lastname: Sperandio
authors.forename: Luiz Fernando Machado
authors.initials: LF
authors.lastname: Silveira
authors.forename: Lenita Aver
authors.initials: LA
authors.lastname: de Araújo
authors.forename: Josué
authors.initials: J
authors.lastname: Martos
authors.forename: Ashwin
authors.initials: A
authors.lastname: Malshe
mesh_terms: D000818:Animals; D001641:Bicuspid / Q000473:pathology; D001672:Biocompatible Materials / Q000627:therapeutic use*; D019343:Citric Acid / Q000627:therapeutic use*; D004285:Dogs; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004492:Edetic Acid / Q000627:therapeutic use*; D023421:Models, Animal; D008963:Molar / Q000473:pathology; D010485:Periapical Periodontitis / Q000139:chemically induced / Q000473:pathology; D010486:Periapical Tissue / Q000187:drug effects* / Q000473:pathology; D011670:Pulpectomy / Q000379:methods; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D013997:Time Factors; D014664:Vasodilation / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid