Another formula for calculating the gene change rate in real-time RT-PCR.
Relative quantification real-time PCR has become a routine method in molecular biology research to study the small amount gene expression. There are a few mathematical models for relative quantification data analysis in real-time PCR, and the formula 2(-DDCT ) is one of the most frequently used method. In this paper, we are to present another equation which directly calculates the change rate (R (c)) of gene expression, and give a example to compare the application of this model with 2(-DDCT).
Chang
Shujian
S
Department of Gastroenterology, The First Affiliated Hospital, Soochow University, 215006 Suzhou, China.
Chen
Weichang
W
Yang
Jicheng
J
D000465:Algorithms; D015870:Gene Expression; D019073:HT29 Cells; D006801:Humans; D008957:Models, Genetic; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D012333:RNA, Messenger