HIV type 1 genetic diversity in Moyale, Mandera, and Turkana based on env-C2-V3 sequences.
The genetic diversity of HIV-1 subtypes circulating in three districts of northern Kenya, i.e., Turkana, Mandera, and Moyale, was studied. DNA sequences encoding a portion of the env-C2-V3 region of the virus were amplified by PCR and sequenced directly. One hundred and fifty-nine samples were successfully sequenced in the env-C2-V3 region and analyzed. From the analysis, 57% were subtype A1, 27% were subtype C, 9% were subtype D, and the remaining 7% were unclassified. This study showed that HIV-1 subtype A1 was the dominant subtype in circulation in this region, though there was a significant percentage of HIV-1 subtype C in circulation there.
Khamadi
Samoel A
SA
Kenya Medical Research Institute, Nairobi, Kenya.
Lihana
Raphael W
RW
Mwaniki
D L
DL
Kinyua
Joyceline
J
Lagat
Nancy
N
Carter
Jane Y
JY
Ichimura
Hiroshi
H
Oishi
Isao
I
Okoth
Fredrick A
FA
Ochieng
Washington
W
D002648:Child; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D017422:Sequence Analysis, DNA; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
D004279:DNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus