X-ray crystallography of agonist/antagonist-bound receptors.
Crystallographic analysis of the ligand-binding domains of nuclear hormone receptors (NR) has provided a unique insight into the molecular events that underlie the ligand-mediated control of their transcriptional activity. The technique relies on preparing milligram quantities of protein, growing three-dimensional crystals of the desired protein-ligand complex, collecting X-ray diffraction data, and subsequently interpreting the derived electron density maps to reveal the structure of the complex.
Pike
Ashley C W
AC
Structural Genomics Consortium, University of Oxford, Headington, Oxford, UK.
D003460:Crystallization / Q000295:instrumentation / Q000379:methods; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D047628:Estrogen Receptor alpha / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D011487:Protein Conformation; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism
D047628:Estrogen Receptor alpha; D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear