Label-free determination of protein-surface interaction kinetics by ionic conductance inside a nanochannel.
We have developed a label-free conductometric platform for the rapid measurement of kinetics parameters for the adsorption and desorption of proteins on surfaces. Adsorbed Bovine Serum Albumin (BSA) has been detected electrically with response times in the minute range, and kinetic models were elaborated and compared to the measurements. The device presents similar characteristics to Surface Plasmon Resonance (SPR) immunosensors, but takes advantage of a simpler, low-cost electronic measurement unit.
Durand
Nicolas F Y
NF
Microsystems Laboratory, STI-LMIS, EPFL, CH-1015, Lausanne, Switzerland.
Renaud
Philippe
P
D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D049329:Nanostructures / Q000737:chemistry; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties
D011506:Proteins; D012710:Serum Albumin, Bovine