Modulation of inflammatory mediators by coumarinolignoids from Cleome viscosa in female swiss albino mice.
The effect of coumarinolignoid cleomiscosins A, B and C isolated from the plant Cleome viscosa on inflammatory mediators were studied in female swiss albino mice. A mixture of coumarinolignoid A, B, and C at 10, 30 and 100 mg/kg body weight once a day for 14 consecutive days were administered orally to the mice. Pro-inflammatory mediators such as IL-6, TNF-alpha and nitric oxide were estimated from culture supernatant obtained from peritoneal macrophages stimulated by LPS and anti-inflammatory mediator IL-4 was estimated from culture supernatant obtained from spleenocytes stimulated by Con-A. For further confirmation, expressions of inflammatory mediators from serum and mortality rate were studied in LPS-induced toxicity model in mice. The expression of Pro-inflammatory mediators was significantly (P <0.05) decreased in coumarinolignoids treatment group in dose dependent manner, whereas the anti-inflammatory mediator expression was significantly increased in coumarinolignoids at 10 mg/kg treatment. Mortality rate was also significantly reduced in treatment group in LPS-induced toxicity model. The result of this study concluded that the oral administration of coumarinolignoids inhibited the pro-inflammatory mediators and enhances the production of anti-inflammatory mediator in dose dependent manner.
Bawankule
D U
DU
Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CIMAP), Kukrail Picnic Spot Road, Lucknow 226015, India. du.bawankule@cimap.res.in
Chattopadhyay
S K
SK
Pal
A
A
Saxena
K
K
Yadav
S
S
Faridi
U
U
Darokar
M P
MP
Gupta
A K
AK
Khanuja
S P S
SP
D000818:Animals; D031485:Cleome / Q000737:chemistry*; D003374:Coumarins / Q000494:pharmacology*; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism*; D015847:Interleukin-4 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D003374:Coumarins; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D009569:Nitric Oxide