Efficient biosensor interfaces based on space-controlled self-assembled monolayers.
In this paper we demonstrate control over the spacing of surface-modifying probe molecules through the use of labile dendron spacers. During this process, anchor molecules are first adsorbed to a surface, with dendron modifiers attached. Steric interactions of the bulky dendrons control the density of anchor molecules bound to the surface. The dendron branches are subsequently detached from the anchor molecules, and the anchors are chemically modified with probe molecules, resulting in a surface with controlled spacing between probe molecules. Control over this spacing is important when the probe size is small in comparison with the target molecule. This importance is demonstrated for the binding of protein (streptavidin) targets to the probe (biotin) surface. The effect of probe space control on the efficiency of target capture is evaluated by examining the binding of streptavidin to thiolated biotin for a series of mixed monolayers. Surface modification is monitored by Fourier transform infrared reflection absorption spectroscopy (FTIR-RAS). The relative concentration of probe molecules at the surface is measured using X-ray photoelectron spectroscopy (XPS) measurements. Thiolated-biotin surfaces with optimized spacing show an increased capture efficiency for streptavidin relative to surfaces with nonoptimal or no control over probe spacing, as measured by surface plasmon resonance (SPR) spectroscopy. These results are of potential significance for the optimization and fabrication of micro- and nanoarrays used in chemical and biochemical measurements.
Tokuhisa
Hideo
H
Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
Liu
Jun'an
J
Omori
Kazuhiro
K
Kanesato
Masatoshi
M
Hiratani
Kazuhisa
K
Baker
Lane A
LA
D015394:Molecular Structure; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance / Q000379:methods*