title: [Adult celiac disease and intraepithelial lymphocytes. New options for diagnosis?].
abstract: BACKGROUND
  Permanent changes have been found in intraepithelial lymphocyte (IEL) subsets in patients with celiac disease.
  
  
  OBJECTIVE
  The main aim of this study was to demonstrate the utility of determining CD3()/CD7(+) and T cell receptor (TCR) gamma-delta IEL subsets by flow cytometry as a diagnostic marker of adult celiac disease.
  
  
  METHODS
  We performed a prospective study in a sample of 128 adult patients (70 with celiac disease and 58 controls). In all patients, distal duodenal biopsy was performed and IEL subsets were determined by flow cytometry.
  
  
  RESULTS
  Patients with celiac disease showed an increase in gamma-delta IEL subsets and a decrease in CD3(-)/CD7(+) IEL subsets in comparison with the control group, independently of diet.
  
  
  CONCLUSIONS
  The results indicate that IEL subset determination by flow cytometry could be useful to confirm diagnosis of celiac disease. IEL subsets should be investigated in diseases other than celiac disease, as well as in patients with potential or latent celiac disease.
authors.affiliation: Servicio de Aparato Digestivo, Hospital Universitario Ramón y Cajal, Madrid, España. pilarolivencia@yahoo.es
authors.forename: Pilar
authors.initials: P
authors.lastname: Olivencia Palomar
authors.forename: Ana
authors.initials: A
authors.lastname: Cano Ruiz
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Martín Scapa
authors.forename: Francisco
authors.initials: F
authors.lastname: León Prieto
authors.forename: Garbiñe
authors.initials: G
authors.lastname: Roy Ariño
authors.forename: Clara
authors.initials: C
authors.lastname: Redondo Verge
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019011:Antigens, CD7 / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D002446:Celiac Disease / Q000178:diet therapy / Q000473:pathology*; D055050:Diet, Gluten-Free; D004384:Duodenoscopy; D004386:Duodenum / Q000276:immunology / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000473:pathology; D006681:HLA-D Antigens / Q000032:analysis; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000473:pathology*; D055815:Young Adult
chemical_list: D019011:Antigens, CD7; D017252:CD3 Complex; D006681:HLA-D Antigens; D016692:Receptors, Antigen, T-Cell, gamma-delta