title: [Increased expression of transient receptor potential melastatin 7 in mouse cardiac fibroblasts post myocardial infarction].
abstract: OBJECTIVE
  To observe transient receptor potential melastatin 7-like (TRPM7L) expression changes post myocardial infarction (MI) in mouse cardiac fibroblast (CF).
  
  
  METHODS
  TRPM7 expression and Ca2+ influx in CF from MI and control mice were quantified by mRNA RT-PCR and whole cell patch clamp technique.
  
  
  RESULTS
  (1) TRPM7 expression was significantly upregulated post MI and Ca2+ influx of CF were significantly increased post MI [(7.4 +/- 0.7) pA/pF vs. (16.2 +/- 1.7) pA/pF, P < 0.01] and Ca2+ influx of CF increased 3-fold under lower pH condition; (2) These effects could be blocked by knock-out TRPM7 gene with SiRNA.
  
  
  CONCLUSIONS
  TRPM7L upregulation post MI and under lower pH condition are responsible for increased Ca2+ influx in CF.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China. li_mingjiang@hotmail.com
authors.forename: Ming-jiang
authors.initials: MJ
authors.lastname: Li
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Zhou
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Cao
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D051379:Mice; D032386:Myoblasts, Cardiac / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000235:genetics; D050053:TRPM Cation Channels / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D050053:TRPM Cation Channels; C421903:Trpm7 protein, mouse; D002118:Calcium