title: [Detection of human papillomavirus in tissues of esophageal carcinomas by polymerase chain reaction].
abstract: OBJECTIVE
  To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinomas.
  
  
  METHODS
  We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5+/6+ for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well.
  
  
  RESULTS
  29 from 31 esophageal carcinoma samples were HPV positive and the rate was 93.5%. Among the samples detected, 19 were HPV16E6 positive and rate was 61.3%, eight were HPV18 E6 positive and rate was 25.8%; our result also showed five were the multiple infection containing HPV16 and 18 as well and the rate was 71.0%.
  
  
  CONCLUSIONS
  The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Linzhou City, Henan Province.
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Li-Dong
authors.initials: LD
authors.lastname: Wang
authors.forename: Xiao-Zhou
authors.initials: XZ
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhao
authors.forename: Hong-Tu
authors.initials: HT
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D036022:Capsid Proteins / Q000235:genetics; D002277:Carcinoma / Q000821:virology*; D002681:China; D017931:DNA Primers / Q000235:genetics; D004938:Esophageal Neoplasms / Q000821:virology*; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral