title: The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation.
abstract: OBJECTIVE
  To measure serum cholinesterase (SCHE) with an integration strategy.
  
  
  METHODS
  At 54.0 micromol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 micromol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 60%, or were determined by the classical initial rate method.
  
  
  RESULTS
  The linear range was from 16 to 1560 nkat/L, and SCHE in clinic sera showed negligible substrate-activation.
  
  
  CONCLUSIONS
  This strategy was effective.
authors.affiliation: Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing 400016, China. liaofeish@yahoo.com
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
authors.forename: Diyuan
authors.initials: D
authors.lastname: Yang
authors.forename: Jingqun
authors.initials: J
authors.lastname: Tang
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.forename: Beizhong
authors.initials: B
authors.lastname: Liu
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Lina
authors.initials: L
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Liao
authors.forename: Ming'an
authors.initials: M
authors.lastname: Yu
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002802:Cholinesterases / Q000097:blood*; D006801:Humans
chemical_list: D002802:Cholinesterases