Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein.
The non-structural protein NS-1 of porcine parvovirus (PPV) could be a useful antigen for differentiating pigs infected with PPV from those vaccinated with inactivated whole virus. NS-1 was expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level, was similar antigenically to the native NS-1 protein as determined by Western blot assay using polyclonal antibodies from pigs vaccinated with alive PPV vaccine. A simple procedure was used to purify the fusion protein. This research lays the foundation for using the NS-1 protein for clinical diagnosis of pigs infected with PPV vs. those vaccinated with inactivated whole virus vaccine.
Qi
Ting
T
Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 15001, China.
Cui
Shang-Jin
SJ
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary; D028323:Parvovirus, Porcine / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*
D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins