title: Nitric oxide and the release of lipoprotein lipase from white adipose tissue.
abstract: OBJECTIVE
  Lipoprotein lipase (LPL) is the main enzyme responsible for the distribution of circulating triacylglycerides in tissues. Its regulation via release from active sites in the vascular endothelium is poorly understood. In a previous study we reported that in response to acute immobilization (IMMO), LPL activity rapidly increases in plasma and decreases in white adipose tissue (WAT) in rats. In other stress situations IMMO triggers a generalized increase in nitric oxide (NO) production.
  
  
  RESULTS
  Here we demonstrate that in rats: 1) in vivo acute IMMO rapidly increases NO concentrations in plasma 2) during acute IMMO the WAT probably produces NO via the endothelial isoform of nitric oxide synthase (eNOS) from vessels, and 3) epididymal WAT perfused in situ with an NO donor rapidly releases LPL from the endothelium.
  
  
  CONCLUSIONS
  We propose the following chain of events: stress stimulus / rapid increase of NO production in WAT (by eNOS) / release of LPL from the endothelium in WAT vessels. This chain of events could be a new mechanism that promotes the rapid decrease of WAT LPL activity in response to a physiological stimulus.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Ricart-Jané
authors.forename: Albert
authors.initials: A
authors.lastname: Casanovas
authors.forename: Núria
authors.initials: N
authors.lastname: Jané
authors.forename: M Alba
authors.initials: MA
authors.lastname: González
authors.forename: Irma
authors.initials: I
authors.lastname: Buira-Morell
authors.forename: Joan
authors.initials: J
authors.lastname: Ribera
authors.forename: Miquel
authors.initials: M
authors.lastname: Llobera
authors.forename: M Dolores
authors.initials: MD
authors.lastname: López-Tejero
mesh_terms: D052436:Adipose Tissue, White / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D004822:Epididymis / Q000201:enzymology; D007103:Immobilization; D008071:Lipoprotein Lipase / Q000097:blood / Q000378:metabolism*; D008297:Male; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010477:Perfusion; D010537:Peritoneum / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D009566:Nitrates; D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D008071:Lipoprotein Lipase