title: Vaginal lactic acid bacteria in healthy and ill bitches and evaluation of in vitro probiotic activity of selected isolates.
abstract: The concentrations of lactic acid bacteria (LAB) in 42 vaginal samples from healthy and ill bitches were determined. Eight isolates belonging to the genera Lactobacillus and Enterococcus were selected and identified and their in vitro antimicrobial activity against canine pathogens and their ability to adhere to canine vaginal epithelial cells were determined. There was no correlation between the concentrations of vaginal LAB and clinical status, body temperature, vaginal pH, or age. Although the animals were either well or suffering from various illnesses, LAB were found in almost every sample, and the selected isolates showed promising probiotic-related features. These findings are significant for the design of strategies for the modulation of vaginal microbiota by vaginal LAB isolates.
authors.affiliation: Departamento de Pequeños Animales, Facultad de Veterinaria, Universidad de República, Lasplaces 1550, Montevideo, Uruguay.
authors.forename: Luis
authors.initials: L
authors.lastname: Delucchi
authors.forename: Martín
authors.initials: M
authors.lastname: Fraga
authors.forename: Karen
authors.initials: K
authors.lastname: Perelmuter
authors.forename: Esther
authors.initials: E
authors.lastname: Cidade
authors.forename: Pablo
authors.initials: P
authors.lastname: Zunino
mesh_terms: D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion / Q000502:physiology*; D015169:Colony Count, Microbial / Q000662:veterinary; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D016983:Enterococcus / Q000302:isolation & purification / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000254:growth & development; D005260:Female; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D019936:Probiotics; D011549:Pseudomonas / Q000254:growth & development; D013211:Staphylococcus aureus / Q000254:growth & development; D014621:Vagina / Q000382:microbiology*