Effects of sodium bicarbonate on butyric acid-induced epithelial cell damage in vitro.
Butyric acid is detected in periodontal pockets and is thought to be involved in the initiation and progression of periodontal disease. We examined the effects of sodium bicarbonate on the butyric acid-induced epithelial cell damage. The human gingival carcinoma cell line Ca9-22 was cultured in medium that contained butyric acid with or without sodium bicarbonate. The viability of cells treated with sodium bicarbonate was significantly higher than that of cells treated with butyric acid alone. The effects of butyric acid on ICAM-1 expression were significantly improved by sodium bicarbonate. Within the limitations of this in vitro study, sodium bicarbonate was indicated to be a useful therapeutic agent to reduce the butyric acid-induced periodontal tissue damage.
Takigawa
Satoko
S
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
Sugano
Naoyuki
N
Ochiai
Kuniyasu
K
Arai
Noriyuki
N
Ota
Noriko
N
Ito
Koichi
K
D002021:Buffers; D020148:Butyric Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002277:Carcinoma / Q000473:pathology; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005434:Flow Cytometry; D005881:Gingiva / Q000187:drug effects* / Q000473:pathology; D005887:Gingival Neoplasms / Q000473:pathology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017693:Sodium Bicarbonate / Q000494:pharmacology*
D002021:Buffers; D003470:Culture Media; D020148:Butyric Acid; D018799:Intercellular Adhesion Molecule-1; D017693:Sodium Bicarbonate