title: Regulation of adrenoceptor and muscarinic receptor gene expression after single and repeated stress.
abstract: Although stress is tightly connected with elevated levels of catecholamines, stress effects on target structures of catecholamine action--adrenoceptors (ARs)--has not been deeply studied yet. Similarly, very little is known about changes of muscarinic receptors (MRs) during stress. We determined changes in these receptors in the individual parts of the heart (right atria and ventricles) of animals (rats and mice) exposed to a single and repeated immobilization stress. Changes of tissue catecholamines, beta(2)-AR gene expression, protein levels, and binding sites were determined in rat right ventricles, and changes in beta(1)-, beta(2)-, and beta(3)-AR gene expression were followed in murine right atria. Tissue catecholamines were elevated, while beta(2)-AR mRNA levels and beta(2)-AR proteins and binding were decreased, in rat right ventricles. In murine right atria, beta(1)- and beta(2)-AR gene expression was elevated, while beta(3)-AR mRNA levels and M(2)-MR were reduced. Taken together, our data show that interaction of AR and MR is important for the organism coping with stress and that different heart regions reveal distinct reactions to stress.
authors.affiliation: Institute of Physiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic. jmys@lf1.cuni.cz
authors.forename: Jaromir
authors.initials: J
authors.lastname: Myslivecek
authors.forename: Andrej
authors.initials: A
authors.lastname: Tillinger
authors.forename: Martina
authors.initials: M
authors.lastname: Novakova
authors.forename: Richard
authors.initials: R
authors.lastname: Kvetnanský
mesh_terms: D000818:Animals; D004837:Epinephrine / Q000378:metabolism; D005786:Gene Expression Regulation; D006325:Heart Atria / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000235:genetics / Q000378:metabolism; D012149:Restraint, Physical; D013315:Stress, Psychological
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic; D009638:Norepinephrine; D004837:Epinephrine