Parallel synthesis and screening of polymers for nonviral gene delivery.
We describe the parallel synthesis and in vitro evaluation of a cationic polymer library for the discovery of nonviral gene delivery vectors. The library was synthesized based on the ring-opening polymerization reaction between epoxide groups of diglycidyl ethers and the amines of (poly)amines. Parallel screening of soluble library constituents led to the identification of lead polymers with high DNA-binding efficacies. Transfection efficacies of lead polymers were evaluated using PC3-PSMA human prostate cancer cells and murine osteoblasts in the absence and presence of serum. In vitro experiments resulted in the identification of a candidate polymer that demonstrated significantly higher transfection efficacies and lower cytotoxicities than poly(ethyleneimine) (pEI), the current standard for polymeric transfection agents. In addition, polymers that demonstrated moderately higher and comparable transfection efficacies with respect to pEI were also identified. Our results demonstrate that high-throughput synthesis and screening of polymers is a powerful approach for the identification of novel nonviral gene delivery agents.
Barua
Sutapa
S
Department of Chemical Engineering, Arizona State University, Tempe, Arizona 85287-6006, USA.
Joshi
Amit
A
Banerjee
Akhilesh
A
Matthews
Dana
D
Sharfstein
Susan T
ST
Cramer
Steven M
SM
Kane
Ravi S
RS
Rege
Kaushal
K
D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000235:genetics; D006801:Humans; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D019076:Transgenes / Q000235:genetics*
D002412:Cations; D011108:Polymers; D004247:DNA