Influence of single-nucleotide polymorphisms on C-reactive protein levels in chronic kidney disease before and after kidney transplantation.
BACKGROUND
We sought to evaluate 2 single-nucleotide polymorphisms (SNPs) in the C-reactive protein (CRP) gene promoter region for their effects on CRP levels in chronic kidney disease (CKD) patients before and after a successful kidney transplantation.


METHODS
Fifty CKD patients were evaluated before and at the first and second years after the graft. Two SNPs were studied, a bi-allelic (G-->A) at the -409 and a tri-allelic (C-->T-->A) variation at the -390 position in the CRP gene.


RESULTS
All patients presented the -409GG genotype. At the -390 position, the "A" allele was not found; there were 15 "CC" patients, 11 "TT" patients, and 24 "CT" patients. CRP levels were different among patients with various genotypes (P < .019). Also the presence of the allele "T" was sufficient to determine differences in CRP levels both in pretransplantation (P = .045) and at 1 year posttransplantation (P = .011), but not at the second year (P = .448).


CONCLUSIONS
SNPs at the -390 position of the CRP gene promoter region influence CRP basal levels in such a way that the "C" allele correlated with the lowest and the "T" with the highest. We did not observe this influence in our patients at the second year posttransplantation.
Cordeiro
A C
AC
Universidade de São Paulo, São Paulo, Brazil.
de Castro
I
I
Pecoits-Filho
R
R
Ramasawmy
R
R
Rodrigues
H
H
Borba
S C P
SC
Pereira
L M
LM
Romão
J E
JE
David-Neto
E
E
Ianhez
L E
LE
Kalil
J
J
Abensur
H
H
D000328:Adult; D002097:C-Reactive Protein / Q000235:genetics* / Q000378:metabolism; D002102:Cadaver; D017931:DNA Primers; D005260:Female; D005500:Follow-Up Studies; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000601:surgery*; D016030:Kidney Transplantation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014019:Tissue Donors
D017931:DNA Primers; D002097:C-Reactive Protein