title: [Mesenchymal stem cells derived from human umbilical cord tissue modulate the secretion of antiplatelet antibody from splenocytes of ITP patients in vitro].
abstract: The study was aimed to investigate the potential immunotherapeutical values of umbilical cord tissue-derived mesenchymal stem cells (UC-MSC) on patients with chronic idiopathic thrombocytopenic purpura (ITP). UC-MSC was cocultured in vitro with splenocytes isolated from ITP patients who experienced splenectomy. The level of IgG antiplatelet antibody (PAIgG) was determined by a competitive micro-enzyme-linked immunosorbent assay (ELISA) method. The proliferation of platelet-reactive CD4+ T lymphocytes was also measured in the presence of UC-MSCs. The results showed that UC-MSCs could stimulate the spontaneous secretion of PAIgG in supernatants; In the platelet-inducing condition, UC-MSC inhibited the production of PAIgG at a low ratio of 1 UC-MSC to 100 splenocytes, but promoted at a high proportion of 1 UC-MSC to 10 splenocytes. Moreover, UC-MSC exerted a suppressive effect on proliferation of platelet-reactive T helper cells in a dose-dependent manner. It is concluded that the UC-MSCs can regulate secretion of antiplatelet antibodies in vitro. Its concrete regulation mechanism and potential immunotherapeutical value are need to further study.
authors.affiliation: Institute of Hematology, Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Qiu
authors.forename: Shao-Guang
authors.initials: SG
authors.lastname: Yang
authors.forename: Zhen-Ping
authors.initials: ZP
authors.lastname: Chen
authors.forename: Qin-Jun
authors.initials: QJ
authors.lastname: Zhao
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Chen
authors.forename: Ze-Ping
authors.initials: ZP
authors.lastname: Zhou
authors.forename: Ren-Chi
authors.initials: RC
authors.lastname: Yang
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D001792:Blood Platelets / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D049109:Cell Proliferation; D006801:Humans; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000378:metabolism*; D013154:Spleen / Q000166:cytology; D014470:Umbilical Cord / Q000502:physiology
chemical_list: D000906:Antibodies