Phylogenetic analysis of rotaviruses with predominant G3 and emerging G9 genotypes from adults and children in Wuhan, China.
Prevalence and phylogenetic relatedness of rotaviruses causing diarrheal diseases in children and adults were analyzed in Wuhan, China. During a period between June 2006 and February 2008, group A rotavirus was identified in 24.9% (280/1126) and 7.6% (83/1088) of specimens taken from children and adults, respectively. G3P[8] was the most frequent genotype in both children (66.3%) and adults (62.7%), followed by G1P[8] (20.3% and 26.2%, respectively). G9 was detected in specimens from six children (2.0%) and seven adults (5.6%). The VP7 genes of G3P[8] rotaviruses from children and adults showed extremely high sequence identities to each other (98.9-100%) and also to those of G3 viruses isolated in Wuhan in 2003-2004. In the phylogenetic analysis of the VP7 gene, the G3P[8] rotaviruses in Wuhan were clustered into a single lineage with some G3 viruses, which had been referred to as "the new variant G3" rotaviruses, reported recently in East Asia and Southeast Asia. Similar to G3P[8] rotaviruses, extremely high sequence identities between children and adults were observed for VP7 genes of G1 and G9 rotaviruses. The G9 viruses were clustered in the lineage of globally spreading strains, while G1 viruses were genetically close to those reported previously in China and Japan. These findings indicated the persistence of the variant G3 rotaviruses and spread of G9 rotaviruses derived from the global G9 lineage in Wuhan, and suggested that the rotaviruses were circulating among children and adults, irrelevant to the G types.
Wang
Yuan-Hong
YH
Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei Province, China.
Kobayashi
Nobumichi
N
Zhou
Xuan
X
Nagashima
Shigeo
S
Zhu
Ze-Rong
ZR
Peng
Jin-Song
JS
Liu
Man-Qing
MQ
Hu
Quan
Q
Zhou
Dun-Jin
DJ
Watanabe
Shojiro
S
Ishino
Masaho
M
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D002681:China; D005243:Feces / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D016415:Sequence Alignment; D055815:Young Adult
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus