Bacterial and mammalian cell response to poly(3-sulfopropyl methacrylate) brushes loaded with silver halide salts.
This study investigates the antibacterial and cytotoxic effect of surfaces with sulphonate brushes containing silver salts. By using the same type of samples for both cytotoxicity and antibacterial studies, these two parameters could be compared in a controlled way. The silver was incorporated into the brush in four different forms to enable release of silver ions at different concentrations and different rates. It was found that although the surfaces displayed very good antibacterial properties in buffer solutions, this effect disappeared in systems with high protein content. Similarly, the silver-containing surfaces displayed cytotoxic effects in the absence of serum proteins but this effect was reduced in the presence of serum. The speciation of silver in the different solutions is discussed. Cytotoxic and antibacterial effects are compared at the different silver concentrations released. The implications of a concentration range where silver could be used to kill bacterial without harmful effects on mammalian cells are also discussed and questioned.
Ramstedt
Madeleine
M
Department of Chemistry, Umeå University, Umeå, Sweden. madeleine.ramstedt@chem.umu.se
Ekstrand-Hammarström
Barbro
B
Shchukarev
Andrey V
AV
Bucht
Anders
A
Osterlund
Lars
L
Welch
Martin
M
Huck
Wilhelm T S
WT
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002021:Buffers; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides; D003470:Culture Media; D004058:Diffusion / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology*; D051379:Mice; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011108:Polymers / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D018030:Silver Compounds / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D002021:Buffers; D002712:Chlorides; D003470:Culture Media; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; D018030:Silver Compounds; C520891:poly(3-sulfopropylmethacrylate)