Identification of differential gene expression for Kr1 gene in bread wheat using annealing control primer system.
Intergeneric hybridization is an important strategy to introgress alien genes into common wheat for its improvement. But presence of cross ability barrier mechanism regulated by Kr1 gene played a major destructive role for hybridization than other reported genes. In order to know the underlying molecular mechanism and to dissect out this barrier, a new annealing system, ACP (anneling control primer) system was used in chromosome 5B (containing Kr1 gene) specific Recombinant Inbred Line (RIL) population. Two differentially expressed fragments for Kr1 gene was identified, cloned and sequenced. Further the expression was confirmed by northern blotting analysis. Sequence analysis of the resulted clones revealed classes of putative genes, including stress responsive and signal transduction.
Manickavelu
Alagu
A
Faculty of Horticulture, Chiba University, 648 Matsudo 271-8510, Japan. manicks@yokohama-cu.ac.jp
Koba
Takato
T
Mishina
Kohei
K
Sassa
Hidenori
H
D000483:Alleles; D017931:DNA Primers; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers