[Effect of total flavones from Cuscuta chinensis on expression of Th type-1/Th type-2 cytokines, serum P and PR in abortion rats model].
OBJECTIVE
To study the effect of TFCC on expression of Th type-1/Th type-2 cytokines and PR in rats abortion model, and observe the changes of serum P and PRL.


METHODS
Early abortion rat model was established by Bromocriptine at 6-8 gestation day, the model rats were treated with high and low dosage of TFCC and the progesterone as the positive control. Progesterone and prolactin levels in the serum were measured by radioimmunoassay, the protein expression of PR was inspected by immunohistochemical, RT-PCR was applied to determine the expression of Thl/Th2 mRNA on deciduas.


RESULTS
In the deciduas of model group, IL-4 and IL-10 cytokine contens were clearly lower than that of normal pregnancy group. The difference was notable(P < 0.05), In the deciduas of model group, IFN-gamma and TNF-alpha cytokine contents were clearly higher than that of normal pregnancy group. The difference was notable (P < 0.05), TFCC could up-regulate the level of IL-4 and IL-10, and down-regulate the level of TNF-alpha and IFN-gamma, which could promote P secretion and up regulate PR mRNA expression on deciduas.


CONCLUSIONS
TFCC can regulate endocrinologycal and immunological network equilibration on maternal-fetal face, finally achieve prevention abortion.
Ma
Hong-xia
HX
The First Affiliated Hospital of Guangzhou Medicine college, Guangzhou 510405, China. doctoprmaxia_1974@yahoo.com.cn
You
Zhao-ling
ZL
Wang
Ruo-guang
RG
D000022:Abortion, Spontaneous / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D000818:Animals; D001971:Bromocriptine; D034182:Cuscuta / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism*; D003656:Decidua / Q000378:metabolism*; D005260:Female; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000097:blood / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D047309:Flavones; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D001971:Bromocriptine; D011374:Progesterone; D007371:Interferon-gamma