Hydrostatic pressure promotes Wnt10b and Wnt4 expression dependent and independent on ERK signaling in early-osteoinduced MSCs.
Recent publications have shown that mechanical stress can regulate the direction of stem cell differentiation. The exact mechanobiological effects of pressure on initial osteodifferentiation have not been determined. Here, we show that ERK signaling participates in early osteodifferentiation and plays a positive but non-critical role in mechanotransduction, whereas p38 MAPK is not involved in this process. Moreover, our findings provide evidence that in response to both types of pressure with high sensitivity, Wnt10b mRNA is ERK-dependent whereas Wnt4 mRNA is upregulated by treatment of the inhibitor of ERK signaling. The findings suggest novel mechanisms of the initial biological responses of bone remodeling and regeneration upon mechanical stimuli.
Liu
Jun
J
State Key Laboratory of Oral Diseases, West China College of Stomatology, West China Hospital of Stomatology, Sichuan University, 14#, 3rd Section, Renmin South Road, Chengdu 610041, China.
Zou
Ling
L
Wang
Jun
J
Schuler
Charles
C
Zhao
Zhihe
Z
Li
Xiaoyu
X
Zhang
Jingyi
J
Liu
Yurong
Y
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005786:Gene Expression Regulation; D006874:Hydrostatic Pressure; D008297:Male; D040542:Mechanotransduction, Cellular / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D051153:Wnt Proteins / Q000235:genetics*; D060528:Wnt4 Protein; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
D051153:Wnt Proteins; C501228:Wnt10b protein, rat; D060528:Wnt4 Protein; C555583:Wnt4 protein, rat; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases