Proteomic analysis of mitochondrial proteins of basal and lipolytically (isoproterenol and TNF-alpha)-stimulated adipocytes.
The regulation of adipocyte lipolysis is increasingly believed to influence insulin resistance, in a process that may be associated with mitochondrial dysfunction. However, the molecular basis of the relationship between mitochondrial protein expression, lipolytic responsiveness, and insulin resistance remains unknown. A set of proteins that shows altered abundances in the mitochondria of untreated and treated 3T3-L1 adipocytes with TNF-alpha or isoproterenol was identified. These include the proteins associated with energy production, including fatty acid oxidation, TCA cycle, and oxidative phosphorylation. Proteins associated with oxidative stress dissipation were down-regulated in lipolytically stimulated adipocytes. Lipolytic stimulation with isoproterenol and TNF-alpha, which is also a potent proinflammatory cytokine, showed some noticeable differences in mitochondrial protein expression. For example, isoproterenol markedly enhanced the expression of prohibitin which is involved in the integrity of mitochondria but TNF-alpha did not. These results provide valuable information on mitochondrial dysfunction associated with oxidative stress induced by lipolytic stimulation.
Cho
Si Young
SY
R&D Center, AmorePacific Corporation, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Korea.
Park
Pil Joon
PJ
Shin
Eui Seok
ES
Lee
Jung Ho
JH
Chang
Hui Kyoung
HK
Lee
Tae Ryong
TR
D000255:Adenosine Triphosphate / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D050356:Lipid Metabolism; D051379:Mice; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010766:Phosphorylation; D017510:Protein Folding; D040901:Proteomics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D005227:Fatty Acids; D024101:Mitochondrial Proteins; D014409:Tumor Necrosis Factor-alpha; D000255:Adenosine Triphosphate; D007545:Isoproterenol