title: Pseudomonas putida based amperometric biosensors for 2,4-D detection.
abstract: Amperometric biosensors using Pseudomonas putida cells as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). After the adaptation process of Pseudomonas putida to 2,4-D, cells were immobilized onto the screen printed graphite electrodes (SPG) as well as Clark oxygen probe by gelatin and glutaraldehyde. Optimum pH, temperature, and stability of the biosensor were investigated. Substrate specificities for various phenolic compounds were also searched. In repeatability studies, variation coefficients and standard deviations for both type of systems were calculated; SPG and Clark electrodes were calculated and results are given as a comparison of two systems. Finally, the biosensors were applied to 2,4-D determination in a real herbicide sample.
authors.affiliation: Faculty of Science, Biochemistry Department, Ege University, Bornova-Izmir, Türkiye.
authors.forename: Dilek
authors.initials: D
authors.lastname: Odaci
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.forename: Nurdan
authors.initials: N
authors.lastname: Pazarlioğlu
authors.forename: Roberto
authors.initials: R
authors.lastname: Pilloton
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinçkaya
authors.forename: Azmi
authors.initials: A
authors.lastname: Telefoncu
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000494:pharmacology*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016958:Pseudomonas putida / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015084:2,4-Dichlorophenoxyacetic Acid