title: Association of IL-17RB gene polymorphism with asthma.
abstract: BACKGROUND
  Interleukin (IL)-17E is a member of the IL-17 family, which induces IL-4, IL-5, IL-13, and eotaxin in experimental animals via IL-17 receptor B (IL-17RB). The activation of IL-17RB amplifies allergic-type inflammatory responses by inducing Jun kinase (or JNK), p38 mitogen-activated protein kinase (or MAPK), and nuclear factor-kappaB.
  
  
  OBJECTIVE
  We examined the association of polymorphisms in the IL-17RB gene with asthma susceptibility and investigated the effects of those polymorphisms on the transcription of various IL-17RB isoforms.
  
  
  METHODS
  In total, 954 asthmatic patients or 265 healthy control subjects were screened for polymorphisms in IL-17RB by single-base extension. The messenger RNA expression IL-17RB in B-cell lines derived from patients was also measured by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Direct sequencing of 24 unrelated Korean DNA samples revealed 18 genetic variants, including four insertion/deletions and 14 single-nucleotide polymorphisms (SNPs). Six of the SNPs (-1465G>A, +5661G>A, +6297T>C [Y123Y], +13797C>T, +18661C>T, and +18965G>A) were used to screen a larger group of subjects. Intronic polymorphism +5661G>A was significantly associated with the development of asthma (p = 0.001); moreover, a minor allele of IL-17RB +5661G>A appeared at a lower frequency in the asthmatic patients than in the healthy control subjects (0.13 vs 0.19, respectively). The IL-17RB messenger RNA expression in B cells homozygous for IL-17RB+ 5661GG was significantly higher than that in B cells homozygous for IL-17RB+5661AA (p = 0.002).
  
  
  CONCLUSIONS
  A rare allele of IL-17RB +5661G>A may have a protective role against the development of asthma via regulation at the level of transcription. The SNPs identified in this study may be used to develop markers to assess the risk of asthma.
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Jung
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc, Seoul, Korea; Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea; Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Byung Lae
authors.initials: BL
authors.lastname: Park
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc, Seoul, Korea.
authors.forename: Hyun Sub
authors.initials: HS
authors.lastname: Cheong
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc, Seoul, Korea.
authors.forename: Joon Seol
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Hun Soo
authors.initials: HS
authors.lastname: Chang
authors.affiliation: Hanyang University, Seoul, Korea.
authors.forename: TaiYoun
authors.initials: T
authors.lastname: Rhim
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Jong-Sook
authors.initials: JS
authors.lastname: Park
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: An-Soo
authors.initials: AS
authors.lastname: Jang
authors.affiliation: Division of Allergy and Respiratory Medicine, Soonchunhyang University Seoul Hospital, Seoul, Korea.
authors.forename: Young-Mok
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Division of Allergy and Respiratory Medicine, Soonchunhyang University Seoul Hospital, Seoul, Korea.
authors.forename: Ki-Up
authors.initials: KU
authors.lastname: Kim
authors.affiliation: Division of Allergy and Respiratory Medicine, Soonchunhyang University Seoul Hospital, Seoul, Korea.
authors.forename: Soo-Taek
authors.initials: ST
authors.lastname: Uh
authors.affiliation: Division of Allergy and Respiratory Medicine, Soonchunhyang University Chunan Hospital, Chunan, Korea.
authors.forename: Ju Ock
authors.initials: JO
authors.lastname: Na
authors.affiliation: Division of Allergy and Respiratory Medicine, Soonchunhyang University Chunan Hospital, Chunan, Korea.
authors.forename: Yong-Hoon
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea. Electronic address: mdcspark@unitel.co.kr.
authors.forename: Choon-Sik
authors.initials: CS
authors.lastname: Park
authors.affiliation: Department of Life Science, Sogang University, Seoul, Korea.
authors.forename: Hyoung Doo
authors.initials: HD
authors.lastname: Shin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001249:Asthma / Q000235:genetics* / Q000503:physiopathology; D001483:Base Sequence; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D053722:Receptors, Interleukin-17 / Q000235:genetics*; D014158:Transcription, Genetic; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D053722:Receptors, Interleukin-17; D048051:p38 Mitogen-Activated Protein Kinases