Influence of protoporphyrin IX amino acid substituents on affinity to human breast adenocarcinoma MCF-7 cells.
The ability of protoporphyrin IX (PP IX) to enter neoplastic cells is used for diagnosis and photodynamic therapy. We present here the effects of PP IX and three amino acid derivatives on MCF-7 cell viability. The fluorescence of each substance was examined using confocal microscopy. Our results show that diarginine diprotoporphyrinate (PP(Arg)2) has satisfactory fluorescence to evaluate the rate of accumulation within cells. We have determined the times at which the accumulation begins and reaches a plateau, as well as cellular localization of PP(Arg)2 in MCF-7 cells.
Misiewicz-Krzemińska
I
I
National Medicines Institute, Chelmska 30/34, Warsaw, Poland. misiewicz@il.waw.pl
Skupińska
K
K
Graczyk
A
A
Kasprzycka-Guttman
T
T
D000599:Amino Acids, Diamino; D001120:Arginine; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D008856:Microscopy, Fluorescence; D011524:Protoporphyrins / Q000493:pharmacokinetics*
D000599:Amino Acids, Diamino; D011524:Protoporphyrins; D001120:Arginine; C028025:protoporphyrin IX