title: Developing and optimizing an immunoaffinity cleanup technique for determination of quinolones from chicken muscle.
abstract: An immunoaffinity chromatographic method was developed using an antibody mediated immunosorbent to selectively extract and purify 10 quinolones (marbofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, difloxacin, sarafloxacin, oxolinic acid, and flumequine) in chicken muscle followed by HPLC. The operating conditions of the immunoaffinity chromatography (IAC) column were optimized, and the IAC has been successfully used for the isolation and purification of 10 quinolones from chicken muscle tissue. The optimized immunoaffinity column sample cleanup procedure combined with HPLC coupling to fluorescence detection afforded low limits of detection (0.1 ng g(-1) for danfloxacin and 0.15 ng g(-1) for all other quinolones tested). The method was also applied to determine quinolone residues in commercial muscle samples.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing.
authors.forename: Sijun
authors.initials: S
authors.lastname: Zhao
authors.forename: Xuelian
authors.initials: X
authors.lastname: Li
authors.forename: Younkyoung
authors.initials: Y
authors.lastname: Ra
authors.forename: Cun
authors.initials: C
authors.lastname: Li
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Li
authors.forename: Zhina
authors.initials: Z
authors.lastname: Qu
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Fangyang
authors.initials: F
authors.lastname: He
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wan
authors.forename: Caiwei
authors.initials: C
authors.lastname: Feng
authors.forename: Zengren
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D007118:Immunoassay / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis*
chemical_list: D015363:Quinolones