Targeting nanodisks via a single chain variable antibody--apolipoprotein chimera.
Nanodisks (ND) are nanometer scale complexes of phospholipid and apolipoprotein that have been shown to function as drug delivery vehicles. ND harboring significant quantities of the antifungal agent, amphotericin B, or the bioactive isoprenoid, all trans retinoic acid, have been generated and characterized. As currently formulated, ND possess limited targeting capability. In this study, we constructed a single chain variable antibody (scFv).apolipoprotein chimera and assessed the ability of this fusion protein to form ND and recognize the antigen to which the scFv is directed. Data obtained revealed that alpha-vimentin scFv.apolipoprotein A-I is functional in ND formation and antigen recognition, opening the door to the use of such chimeras in targeting drug-enriched ND to specific tissues.
Iovannisci
David M
DM
Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
Beckstead
Jennifer A
JA
Ryan
Robert O
RO
D000666:Amphotericin B / Q000737:chemistry / Q000378:metabolism; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism; D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D014677:Pharmaceutical Vehicles / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism; D014212:Tretinoin / Q000737:chemistry / Q000378:metabolism
D000935:Antifungal Agents; D016632:Apolipoprotein A-I; D007135:Immunoglobulin Variable Region; D014677:Pharmaceutical Vehicles; D011993:Recombinant Fusion Proteins; D013729:Terpenes; D014212:Tretinoin; D000666:Amphotericin B