Coculture methodologies for the study of Wnt signals.
In vivo, responses to extracellular Wnt ligands are context dependent; the temporal characteristics and intensity of the signal are critical in determining the target cell response. In general, Wnt ligand-induced differentiation in mammalian cells requires several days of exposure. In order to better characterize Wnt-induced signaling in vitro, side-by-side and partitioned cocultures can be utilized. These methodologies closely mimic how Wnt signals are transmitted in the tumor microenvironment.
Planutis
Kestutis
K
Division of Hematology/Oncology, University of California-Irvine, Orange, USA.
Planutiene
Marina
M
Holcombe
Randall F
RF
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D017077:Culture Media, Conditioned; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
D017077:Culture Media, Conditioned; D051153:Wnt Proteins