title: Human CYP4Z1 catalyzes the in-chain hydroxylation of lauric acid and myristic acid.
abstract: Overexpression of human CYP4Z1, a cytochrome P450 enzyme, has been correlated with poor prognosis in human cancer. However, its catalytic properties are not yet known. We expressed this P450 in Schizosaccharomyces pombe and demonstrate by whole-cell biotransformation assays CYP4Z1-dependent in-chain hydroxylation of lauric and myristic acid, which in both cases leads to the formation of four different monohydroxylated products at positions omega-2, omega-3, omega-4, and omega-5, respectively. The CYP4Z1-expressing fission yeast should be a new valuable tool for testing cancer drugs or for the development of new prodrug strategies.
authors.affiliation: PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany.
authors.forename: Andy
authors.initials: A
authors.lastname: Zöllner
authors.forename: Calin-Aurel
authors.initials: CA
authors.lastname: Dragan
authors.forename: Dominik
authors.initials: D
authors.lastname: Pistorius
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
authors.forename: Helge B
authors.initials: HB
authors.lastname: Bode
authors.forename: Frank T
authors.initials: FT
authors.lastname: Peters
authors.forename: Hans H
authors.initials: HH
authors.lastname: Maurer
authors.forename: Matthias
authors.initials: M
authors.lastname: Bureik
mesh_terms: D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D000072470:Cytochrome P450 Family 4; D006801:Humans; D006900:Hydroxylation; D007850:Lauric Acids / Q000737:chemistry*; D015394:Molecular Structure; D019814:Myristic Acid / Q000737:chemistry*
chemical_list: D007850:Lauric Acids; D019814:Myristic Acid; C030358:lauric acid; D003577:Cytochrome P-450 Enzyme System; C485207:CYP4Z1 protein, human; D000072470:Cytochrome P450 Family 4