Pathogen prevalence and influence of composted dairy manure application on antimicrobial resistance profiles of commensal soil bacteria.
Composting manure, if done properly, should kill pathogenic bacteria such as Salmonella and Escherichia coli O157:H7, providing for an environmentally safe product. Over a 3-year period, samples of composted dairy manure, representing 11 composting operations (two to six samples per producer; 100 total samples), were screened for Salmonella and E. coli O157:H7 and were all culture negative. Nonpathogenic bacteria were cultured from these compost samples that could theoretically facilitate the spread of antimicrobial resistance from the dairy to compost application sites. Therefore, we collected soil samples (three samples per plot; 10 plots/treatment; 90 total samples) from rangeland that received either composted dairy manure (CP), commercial fertilizer (F), or no treatment (control, CON). Two collections were made appoximately 2 and 7 months following treatment application. Soil samples were cultured for Pseudomonas and Enterobacter and confirmed isolates subjected to antimicrobial susceptibility testing. Three species of Enterobacter (cloacae, 27 isolates; aeroginosa, two isolates; sakazakii, one isolate) and two species of Pseudomonas (aeruginosa, 11 isolates; putida, seven isolates) were identified. Five Enterobacter isolates were resistant to ampicillin and one isolate was resistant to spectinomycin. All Pseudomonas isolates were resistant to ampicillin, ceftiofur, florfenicol, sulphachloropyridazine, sulphadimethoxine, and trimethoprim/sulfamethoxazole and most isolates were resistant to chlortetracycline and spectinomycin. Pseudomonas isolates were resistant to an average of 8.6, 7.9, and 8 antibiotics for CON, CP, and F treatments, respectively. No treatment differences were observed in antimicrobial resistance patterns in any of the soil isolates examined. Results reported herein support the use of composted dairy manure as an environmentally friendly soil amendment.
Edrington
Tom S
TS
Southern Plains Agricultural Research Center, U.S. Department of Agriculture, College Station, Texas 77845, USA. edrington@ffsru.tamu.edu
Fox
William E
WE
Callaway
Todd R
TR
Anderson
Robin C
RC
Hoffman
Dennis W
DW
Nisbet
David J
DJ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle / Q000382:microbiology; D015169:Colony Count, Microbial; D004197:Disease Reservoirs / Q000382:microbiology / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D004754:Enterobacter / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D005260:Female; D005506:Food Contamination / Q000517:prevention & control; D008372:Manure / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D011549:Pseudomonas / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D012988:Soil Microbiology
D000900:Anti-Bacterial Agents; D008372:Manure