title: Ion-trap mass spectrometry unveils the presence of isomeric oligosaccharides in an analyte: stage-discriminated correlation of energy-resolved mass spectrometry.
abstract: Mass spectrometry, especially tandem mass spectrometry, has been widely used in the field of analytical sciences for handling biological and chemical samples. The technique resolves molecular and fragment ions based on the mass to charge ratio. Energy-resolved mass spectrometry (ERMS) further provides an activation energy-related factor in the dissociation reaction. Therefore, it is a very powerful technique that can discriminate isomeric compounds. Despite the power of ERMS, useful information cannot be obtained when an analyte contains structural isomers. Carbohydrates carry multiple chiral centers, thus oligomers of monosaccharides can form a vast number of structural isomers. We decided to use such species in our endeavors to establish a method of identifying the 'purity' of an analyte solely based on mass spectrometry. In the present paper, we describe a stage-discriminated spectral correlation of ERMS, which not only enables identification of the presence of contaminants in an analyte, but also provides information regarding the 'purity' of fragment ions.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan.
authors.forename: Shusaku
authors.initials: S
authors.lastname: Daikoku
authors.forename: Ayako
authors.initials: A
authors.lastname: Kurimoto
authors.forename: Sachiko
authors.initials: S
authors.lastname: Mutsuga
authors.forename: Takuro
authors.initials: T
authors.lastname: Ako
authors.forename: Takuya
authors.initials: T
authors.lastname: Kanemitsu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shioiri
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ohtake
authors.forename: Rumiko
authors.initials: R
authors.lastname: Kato
authors.forename: Chikako
authors.initials: C
authors.lastname: Saotome
authors.forename: Isao
authors.initials: I
authors.lastname: Ohtsuka
authors.forename: Satomi
authors.initials: S
authors.lastname: Koroghi
authors.forename: Sujit K
authors.initials: SK
authors.lastname: Sarkar
authors.forename: Akifumi
authors.initials: A
authors.lastname: Tobe
authors.forename: Shin
authors.initials: S
authors.lastname: Adachi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Suzuki
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000145:classification
chemical_list: D009844:Oligosaccharides