Response of neural precursor cells in the brain of Parkinson's disease mouse model after LIF administration.
OBJECTIVE
To access the response of endogenous neural precursor cells (NPCs) in the area of substantia nigra (SN) in the mouse model of Parkinson's disease (PD). To evaluate whether leukemia inhibitory factor (LIF) can up-regulate the expression of NPCs and their fate in differentiation.


METHODS
NPCs were measured and the number and density were estimated using the confocal counting system in normal control (CON), normal control LIF treated (CON + LIF), PD (PD) and LIF treated PD (PD + LIF) mice.


RESULTS
The PD + LIF group showed a statistically significant improvement in the number and density of NPCs compared with PD group, and NPCs are rarely seen in CON and CON + LIF groups.


CONCLUSIONS
LIF may be a useful treatment for PD by up-regulating the re-expression of NPCs, which may represent the neuroprotection mechanism of LIF.
Liu
Juan
J
Microbiology Group, Department of Laboratory Medicine, Tianjin First Central Hospital, Tianjin 300192, China.
Zang
Dawei
D
D018663:Adrenergic Agents / Q000633:toxicity; D053687:Adult Stem Cells / Q000187:drug effects*; D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D016627:Oxidopamine / Q000633:toxicity; D010300:Parkinson Disease / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013378:Substantia Nigra / Q000187:drug effects / Q000473:pathology*; D015854:Up-Regulation / Q000187:drug effects
D018663:Adrenergic Agents; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D018809:Proliferating Cell Nuclear Antigen; D016627:Oxidopamine