title: Stress induces tRNA cleavage by angiogenin in mammalian cells.
abstract: tRNAs play a central role in protein translation, acting as the carrier of amino acids. By cloning microRNAs, we unexpectedly obtained some tRNA fragments generated by tRNA cleavage in the anticodon loop. These tRNA fragments are present in many cell lines and different mouse tissues. In addition, various stress conditions can induce this tRNA cleavage event in mammalian cells. More importantly, angiogenin (ANG), a member of RNase A superfamily, appears to be the nuclease which cleaves tRNAs into tRNA halves in vitro and in vivo. These results imply that angiogenin plays an important physiological role in cell stress response, except for the known function of inducing angiogenesis.
authors.affiliation: Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People's Republic of China.
authors.forename: Hanjiang
authors.initials: H
authors.lastname: Fu
authors.forename: Junjun
authors.initials: J
authors.lastname: Feng
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Tie
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.forename: Ruiyun
authors.initials: R
authors.lastname: Xing
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Sun
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000926:Anticodon / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D051379:Mice; D012343:RNA, Transfer / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D000926:Anticodon; D012343:RNA, Transfer; C046366:angiogenin; D012259:Ribonuclease, Pancreatic