[Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse].
OBJECTIVE
To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.


METHODS
TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.


RESULTS
TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.


CONCLUSIONS
HBX protein could be induced into mouse liver by TAT induced peptide.
Shi
Ying
Y
Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Wei
Fei-Li
FL
Liu
Ya-Li
YL
Ji
Yun-Xia
YX
Wu
Hao
H
Chen
De-Xi
DX
Zhou
Yu-Sen
YS
D000818:Animals; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006509:Hepatitis B / Q000378:metabolism / Q000821:virology; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C485184:enhanced green fluorescent protein; C057628:hepatitis B virus X protein; D054322:tat Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins