title: Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1.
abstract: The accumulation of phosphatidylcholine hydroperoxide (PCOOH), a primary oxidation product of phosphatidylcholine (PC), in blood plasma and tissues has been observed in various pathological conditions, including atherosclerosis. However, the biological roles of PCOOH in these conditions remain unknown. To estimate the atherogenicity of PCOOH, we evaluated the effect of PCOOH on THP-1 monocytic cell adherence to immobilized vascular endothelial cell adhesion molecules. THP-1 cell adhesion to intracellular adhesion molecule-1 (ICAM-1) was dose-dependently increased by addition of PCOOH. Phosphatidylcholine hydroxide (a hydroxyl analog of PCOOH) also induced THP-1 cell adhesion to ICAM-1, whereas nonoxidized PC, sn-2 truncated PCs, and other hydroperoxide compounds did not affect the adhesion. In the PCOOH-treated cells, obvious protruding F-actin-rich membrane structures were formed, and lymphocyte function-associated antigen-1 (LFA-1) was localized to the protruding structures. Cytochalasin D, an actin polymerization inhibitor, suppressed the PCOOH-induced cell adhesion to ICAM-1 and the membrane protrusions. These results indicate that PCOOH evokes LFA-1-mediated cell adhesion to ICAM-1 via actin cytoskeletal organization, and the mechanism may participate in monocyte adherence to the arterial wall in the initiation of atherosclerosis.
authors.affiliation: Department of Medicine, Division of Endocrinology and Metabolism, Nippon Medical School, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Asai
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Okajima
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Daigo
authors.initials: D
authors.lastname: Ibusuki
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tanimura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Mototsugu
authors.initials: M
authors.lastname: Nagao
authors.forename: Mariko
authors.initials: M
authors.lastname: Sudo
authors.forename: Taro
authors.initials: T
authors.lastname: Harada
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oikawa
mesh_terms: D000199:Actins / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D022081:Cell Surface Extensions / Q000378:metabolism; D015638:Cytochalasin D / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000378:metabolism; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000199:Actins; D015703:Antigens, CD; D016169:Lymphocyte Function-Associated Antigen-1; D019384:Nucleic Acid Synthesis Inhibitors; D010713:Phosphatidylcholines; D017382:Reactive Oxygen Species; C057590:phosphatidylcholine hydroperoxide; D018799:Intercellular Adhesion Molecule-1; D015638:Cytochalasin D