Determination of main taxoids in Taxus species by microwave-assisted extraction combined with LC-MS/MS analysis.
A method based on microwave-assisted extraction (MAE) has been developed for the determination of paclitaxel and five related taxoids, namely 10-deacetylbaccatin III (10-DAB III), cephalomannine, 10-deacetylpaclitaxel (10-DAT), 7-xyl-10- deacetylpaclitaxel (7-xyl-10-DAT), and 7-epi-10-deacetylpaclitaxel (7-epi-10-DAT) in Taxus species in this study. The influential parameters of the MAE procedure were optimized, and the optimal conditions were as follows: extraction solvent 80% ethanol solution, solid/liquid ratio 1:10 (g/mL), temperature 50 degrees C, and three extraction cycles, each cycle 10 min. The method validation for LC-MS/MS analysis was performed. The LOD and LOQ were 3.16-9.20 and 12.20-30.45 ng/mL, respectively. Repeatability and reproducibility for the six taxiods with RSD ranged from 2.78 to 3.85% and from 5.26 to 6.60%. The recoveries of the method for the six taxoids were 92.6-105.6%. The developed MAE-LC-MS/MS method was also successfully applied to determine the contents of six taxoids in different Taxus species.
Luo
Hao
H
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China.
Nie
Ying-Kun
YK
Fu
Yu-Jie
YJ
Zu
Yuan-Gang
YG
Li
Shuang-Ming
SM
Liu
Wei
W
Zhang
Lin
L
Luo
Meng
M
Kong
Yu
Y
Li
Zheng-Nan
ZN
D002853:Chromatography, Liquid / Q000379:methods*; D008872:Microwaves; D015394:Molecular Structure; D012997:Solvents; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000032:analysis* / Q000737:chemistry*; D020946:Taxus / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D012997:Solvents; D043823:Taxoids