Effect of ampicillin and chloroquine on humoral immune response elicited by bovine albumin encapsulated in liposomes.
Immune suppression resulting from chemoprophylaxis and potential drug interaction were investigated in experimental animals pre-medicated with ampicillin and chloroquine followed by immunization with bovine serum albumin bearing liposomes prepared by the reverse phase evaporation method. The prepared liposomes were evaluated for particle size, entrapment efficiency and in vitro release. Humoral immune response was measured in terms of systemic IgG antibody titre by the ELISA method. The present study showed that 7:3 molar ratio of soya phosphatidylcholine and cholesterol produced liposomes of mean diameter of 235.4 +/- 10.3 nm and entrapment efficiency of 41.3 +/- 3.2%. Ampicillin significantly (p < 0.05) decreased the antibody titre whereas chloroquine did not reduce the antibody titre significantly. The study will help in programming a new drug management and in characterization of vaccine-drug interaction.
Madan
Jitender
J
Department of Pharmaceutics, Hindu College of Pharmacy, Sonepat (Haryana), India.
Kaushik
Dinesh
D
Sardana
Satish
S
Mishra
Dina Nath
DN
Singh
Shalinder Kumar
SK
Singh
Binod Kumar
BK
D000667:Ampicillin / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002738:Chloroquine / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000097:blood; D008081:Liposomes; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000276:immunology*
D007074:Immunoglobulin G; D008081:Liposomes; D012710:Serum Albumin, Bovine; D000667:Ampicillin; D002738:Chloroquine