Visible-light photocontrol of (E)/(Z) isomerization of the 4-(dimethylamino)azobenzene pseudo-nucleotide unit incorporated into an oligonucleotide and DNA hybridization in aqueous media.
We demonstrate significant photoresponsivity in aqueous media to visible light of pseudo-oligonucleotides possessing 4-(dimethylamino)azobenzene (4-DMAzo) side chains. The spectrum of the 4-DMAzo moiety during 436 nm light irradiation at pH >9 was clearly different from that of the all (E)-form, indicating the presence of the (Z)-form. Thermal (Z)-to-(E) recovery isomerization was faster at pH 9 (k(Z)(-E) = approximately 10(1) s(-1)) than at pH 11; however, addition of 50% ethanol significantly slowed the thermal recovery isomerization at pH 9 (k(Z)(-E) = approximately 2 s(-1)) and increased the magnitude of the spectral changes. Significant photoregulation of DNA hybridization by visible light was demonstrated under this condition.
Kamei
Takashi
T
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan. kameitakashi@es.hokudai.ac.jp
Akiyama
Haruhisa
H
Morii
Hisayuki
H
Tamaoki
Nobuyuki
N
Uyeda
Taro Q P
TQ
D002021:Buffers; D004247:DNA / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008027:Light; D009693:Nucleic Acid Hybridization / Q000528:radiation effects; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry; D004124:p-Dimethylaminoazobenzene / Q000737:chemistry*
D002021:Buffers; D009841:Oligonucleotides; D014867:Water; D000431:Ethanol; D004247:DNA; D004124:p-Dimethylaminoazobenzene