Antigen-specific lymphocyte proliferation assay and virus neutralization test for measurement of measles-specific immunity in 15-19 years old high school students in Tehran, Iran.
Limited information is available concerning the role of measles-specific cell mediated immunity as a correlate of long-term protection from measles infection. Although serological responses are determined in epidemiological studies and high antibody titer is a good indicator of protection, the role of Cell-Mediated Immunity (CMI) has to be defined more clearly. In this study, Lymphocyte Proliferation (LP) and Viral Neutralization Test (VNT) were used in order to measure measles-specific cellular and humoral immune responses of 100 high school students in Tehran. From total number of subjects studied, 33 were girls and 67 were boys and all were in good health. Of these, 77 had protective neutralizing measles antibody titers and 23 did not have such titer. The results of LP showed that 89 subjects had protective cellular immune responses and 11 did not. A quantitative relationship between humoral and cellular immune responses was not observed. These findings suggest that measles-specific protective CMI is measurable for longer time in comparison to humoral immunity. These data suggest that LP responses may be better sustained than antibody titers in some children.
Jamehdar
S Amel
SA
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Sabahi
F
F
Zandi
K
K
Shafiee
A
A
Zadeh
S Faghih
SF
Jalilian
F Azizi
FA
D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000737:chemistry*; D000956:Antigens, Viral / Q000276:immunology*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007492:Iran; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D008297:Male; D008457:Measles / Q000276:immunology* / Q000517:prevention & control*; D008458:Measles Vaccine / Q000276:immunology* / Q000627:therapeutic use; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests / Q000379:methods*; D014611:Vaccination
D000906:Antibodies; D000941:Antigens; D000956:Antigens, Viral; D008458:Measles Vaccine