title: Toward a PCR-independent molecular diagnosis of veterinary and medically relevant pathogenic organisms.
abstract: Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.
authors.affiliation: School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, United Kingdom. Matthew.Partington@ncl.ac.uk
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Partington
authors.forename: Dino
authors.initials: D
authors.lastname: Masic
authors.forename: Audrey
authors.initials: A
authors.lastname: Nicholson
authors.forename: John
authors.initials: J
authors.lastname: Perry
authors.forename: Andrew
authors.initials: A
authors.lastname: Berrington
authors.forename: Jonatan
authors.initials: J
authors.lastname: West
authors.forename: Olivier A E
authors.initials: OA
authors.lastname: Sparagano
mesh_terms: D000818:Animals; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000662:veterinary