[Construction of tissue-engineering skin with carrier of active composite dermal matrix].
OBJECTIVE
To construct of tissue engineering skin including active composite dermal matrix.


METHODS
The human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations.


RESULTS
The structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes.


CONCLUSIONS
Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.
Ma
Zhong-feng
ZF
Burns Institute, the First Affiliated Hospital to the PLA General Hospital, Beijing 100037, PR China.
Chai
Jia-ke
JK
Yang
Hong-ming
HM
Liang
Li-ming
LM
Xu
Ming-huo
MH
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D024042:Collagen Type I; D020405:Dermis / Q000637:transplantation*; D000078404:Epidermal Cells; D005109:Extracellular Matrix / Q000637:transplantation*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D012867:Skin / Q000166:cytology; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering
D024042:Collagen Type I