title: Analysis of cotton (Gossypium hirsutum) root proteomes during a compatible interaction with the black root rot fungus Thielaviopsis basicola.
abstract: A proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with Thielaviopsis basicola. Microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-DE analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein expression levels showed four major profiles (constant, upregulated, one slightly downregulated, and one dramatically downregulated). Spots significantly (p<0.05) upregulated were analyzed by LC-MS/MS and identified using MASCOT MS/MS ion search software and associated databases. These proteins included defense and stress related proteins, such as pathogenesis-related proteins and proteins likely to be involved in the oxidative burst, sugar, and nitrogen metabolism as well as amino acid and isoprenoid synthesis. While many of the identified proteins are common components of the defense response of most plants, a proteasome subunit and a protein reported to be induced only in cotton root following Meloidogyne incognita infection were also identified.
authors.affiliation: Molecular and Cellular Biology, School of Science and Technology, University of New England, Armidale, NSW, Australia. jmoensco@une.edu.au
authors.forename: Joëlle V F
authors.initials: JV
authors.lastname: Coumans
authors.forename: Anne
authors.initials: A
authors.lastname: Poljak
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Raftery
authors.forename: David
authors.initials: D
authors.lastname: Backhouse
authors.forename: Lily
authors.initials: L
authors.lastname: Pereg-Gerk
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D018929:Cell Culture Techniques; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000254:growth & development / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins