title: Prevalence and age-related infection of Cryptosporidium suis, C. muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic.
abstract: A total of 413 pig faecal samples were collected from pre-weaners (119), starters (131), pre-growers (123) and sows (40) from a farm with a closed breeding system segmented into two breeding complexes and a growing complex in the region of Vysocina, Czech Republic and screened for the presence of Cryptosporidium using staining methods and genotyping (SSU rRNA). Cryptosporidium oocysts were detected by microscopy in the faeces of 21.1% of the samples (87/413). Sequence analyses and RFLP identified C. suis in 44, Cryptosporidium pig genotype II in 23 and C. muris in 2 samples. No mixed infections were found. Pigs under 7 weeks of age were infected with C. suis only. Cryptosporidium pig genotype II was found in animals from 7 weeks of age. No relationship was found between diarrhoea and any Cryptosporidium infection in any of the different age groups (P<0.05). The pre-weaned pigs shed significantly more Cryptosporidium oocysts than older pigs and it was associated with C. suis infection.
authors.affiliation: Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, 370 05 Ceské Budejovice, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Kvác
authors.forename: Dagmar
authors.initials: D
authors.lastname: Hanzlíková
authors.forename: Bohumil
authors.initials: B
authors.lastname: Sak
authors.forename: Dana
authors.initials: D
authors.lastname: Kvetonová
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000469:parasitology; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018153:Czech Republic / Q000453:epidemiology; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology*; D005260:Female; D005838:Genotype; D008297:Male; D033661:Oocysts; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics; D012307:Risk Factors; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000469:parasitology; D014886:Weaning
chemical_list: D016054:DNA, Protozoan; D016053:RNA, Protozoan