Polarization of protective immunity induced by replication-incompetent adenovirus expressing glycoproteins of pseudorabies virus.
Replication-incompetent adenoviruses expressing three major glycoproteins (gB, gC, and gD) of pseudorabies virus (PrV) were constructed and used to examine the ability of these glycoproteins to induce protective immunity against a lethal challenge. Among three constructs, recombinant adenovirus expressing gB (rAd-gB) was found to induce the most potent immunity biased to Th1-type, as determined by the IgG isotype ratio and the profile of the Th1/Th2 cytokine production. Conversely, the gC-expressing adenovirus (rAd-gC) revealed Th2-type immunity and the gD-expressing adenovirus (rAd-gD) induced lower levels of IFN-? and IL-4 production than other constructs, except IL-2 production. Mucosal delivery of rAd-gB induced mucosal IgA and serum IgG responses and biased toward Th2-type immune responses. However, these effects were not observed in response to systemic delivery of rAd-gB. In addition, rAd-gB appeared to induce effective protective immunity against a virulent viral infection, regardless of whether it was administered via the muscular or systemic route. These results suggest that administration of replication-incompetent adenoviruses can induce different types of immunity depending on the expressed antigen and that recombinant adenoviruses expressing gB induced the most potent Th1-biased humoral and cellular immunity and provided effective protection against PrV infection.
Han
Young Woo
YW
Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.
Aleyas
Abi G
AG
George
Junu A
JA
Kim
Seon Ju
SJ
Kim
Hye Kyung
HK
Yoon
Hyun A
HA
Yoo
Dong Jin
DJ
Kang
Seong Ho
SH
Kim
Koanhoi
K
Eo
Seong Kug
SK
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013552:Swine; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014779:Virus Replication
D016207:Cytokines; D006023:Glycoproteins; D007074:Immunoglobulin G; D022421:Pseudorabies Vaccines