title: [The correlation between the polyomavirus DNA load and the dose of immunosuppressant].
abstract: OBJECTIVE
  To explore the correlation between the polyomavirus DNA load and the dose of immunosuppressant in patients with allogene bone marrow transplantation (allo-BMT) for preventing the development of post-transplantational hemorrhagic cystitis.
  
  
  METHODS
  Serial blood and urine samples from 122 cases of allo-BMT recipients were obtained and DNA was extracted from urine samples. Polyomavirus DNA-specific probe was synthesized and Fluorescence quantitative polymerase chain reaction was used for detecting the polyomavirus DNA loads and Fluorescence polarization immunoassay (FPIA) was performed for determining the dose of immunosuppressant Cyclosporin A (CsA) in blood.
  
  
  RESULTS
  The altered polyomavirus DNA load in urine was followed by concentration of CsA in blood. When the concentration of CsA in blood was higher than 86-105 ng/ml, the positive rate of polyomavirus DNA load was significantly increased and both presented the linable correlation.
  
  
  CONCLUSIONS
  In immunosuppression condition, polyomavirus DNA load correlated to the dose of immunosuppressant, which increased the risk of post-transplantational hemorrhagic cystitis.
authors.affiliation: Beijing Dao Pei Hospital, Beijing 100039, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Teng
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Wan-Xia
authors.initials: WX
authors.lastname: Zheng
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Xu
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Tong
mesh_terms: D016026:Bone Marrow Transplantation / Q000009:adverse effects; D016572:Cyclosporine / Q000009:adverse effects* / Q000097:blood; D003556:Cystitis / Q000517:prevention & control / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000652:urine; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects* / Q000097:blood; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000517:prevention & control / Q000821:virology*; D014184:Transplantation, Homologous / Q000009:adverse effects; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D007166:Immunosuppressive Agents; D016572:Cyclosporine