Rapid analysis of methamphetamine in hair by micropulverized extraction and microchip-based competitive ELISA.
An automated full-range quantitation method for identifying d-methamphetamine in human hair using a microchip-based ELISA system (microELISA) in combination with a micropulverized extraction method for sample preparation has been developed. An antibody and a peroxidase-linked methamphetamine, both are commercially available, were used for the competitive ELISA assay. Method validation was carried out using doped hair samples, and segmental analyses of real-case specimens were carried out by both microELISA and LC/MS/MS to verify the reliability and applicability of this new method. Due to the small size of the system and the lack of an evaporation process, sample preparation and quantitation can be accomplished easily and quickly (less than 30 min) in small-scale contamination-free environments.
Miyaguchi
Hajime
H
National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. miyaguchi@nrips.go.jp
Takahashi
Hiroko
H
Ohashi
Toshinori
T
Mawatari
Kazuma
K
Iwata
Yuko T
YT
Inoue
Hiroyuki
H
Kitamori
Takehiko
T
D000697:Central Nervous System Stimulants / Q000032:analysis*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D053593:Forensic Toxicology; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008694:Methamphetamine / Q000032:analysis*; D046208:Microchip Analytical Procedures; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods
D000697:Central Nervous System Stimulants; D008694:Methamphetamine