title: Characterization of chemoreceptive protein binding to an oviposition stimulant using a fluorescent micro-binding assay in a butterfly.
abstract: A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated previously from the chemoreceptive organs was assumed to bind to an oviposition stimulant. Using a highly sensitive fluorescent micro-binding assay, we clarified OSBP bound to aristolochic acid. Three-dimensional molecular modeling revealed the structure of the OSBP-aristolochic acid complex. This is the first report of a native chemoreceptive protein binding to an oviposition stimulant as a ligand in insects.
authors.affiliation: Iwaki Meisei University, Faculty of Pharmacy, 5-5-1 Iino, Chuo-dai, Iwaki, Fukushima 970-8551, Japan. tutihara@iwakimu.ac.jp
authors.forename: Kazuko
authors.initials: K
authors.lastname: Tsuchihara
authors.forename: Tetsuichi
authors.initials: T
authors.lastname: Wazawa
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Ishii
authors.forename: Toshio
authors.initials: T
authors.lastname: Yanagida
authors.forename: Ritsuo
authors.initials: R
authors.lastname: Nishida
authors.forename: Xiang-Guo
authors.initials: XG
authors.lastname: Zheng
authors.forename: Masaji
authors.initials: M
authors.lastname: Ishiguro
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Osamu
authors.initials: O
authors.lastname: Hisatomi
authors.forename: Fumio
authors.initials: F
authors.lastname: Tokunaga
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D030022:Aristolochia / Q000378:metabolism; D034341:Aristolochic Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001681:Biological Assay; D002080:Butterflies / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D010058:Oviposition / Q000187:drug effects; D011487:Protein Conformation
chemical_list: C122155:6-bromoacetyl-2-dimethylaminonaphthalene; D034341:Aristolochic Acids; D005456:Fluorescent Dyes; D019476:Insect Proteins; C000228:aristolochic acid I; D015081:2-Naphthylamine