Selective fluorescence quenching of the 8-oxoG-clamp by 8-oxodeoxyguanosine in ODN.
The 8-oxoG-clamp, a specific fluorescent probe for 8-oxo-deoxyguanosine (8-oxo-dG), was incorporated into the oligodeoxynucleotide (ODN) within or at the 3'-end of the purine and the pyrimidine sequences. Based on the UV-melting temperature, the 8-oxoG-clamp showed slightly lower stabilizing effects on the duplexes containing 8-oxo-dG at the complementary site than that with dG. On the other hand, 8-oxo-dG in DNA was selectively detected by fluorescence quenching of the 8-oxoG-clamp.
Nasr
Tamer
T
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Li
Zhichun
Z
Nakagawa
Osamu
O
Taniguchi
Yosuke
Y
Ono
Sayaka
S
Sasaki
Shigeki
S
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001483:Base Sequence; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D015195:Drug Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014466:Ultraviolet Rays
C453560:7,8-dihydro-8-oxoguanine; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine