Prolongation of rat intestinal allograft survival by administration of triptolide-modified donor bone marrow-derived dendritic cells.
Severe graft rejection remains an important obstacle in intestinal transplantation. In this study, dendritic cells (DCs) isolated from rat bone marrow were cultured for 5 days, and triptolide applied for 3 more days. The recipient rats were pretreated with donor triptolide-modified or not modified DC. Small bowel transplantation was performed to observed survival times. We demonstrated that triptolide markedly inhibited both the expression of CD80 and MHCII expression on DCs. Triptolide-modified DCs stimulated lower proliferative responses among allogeneic T cells, prolonging the survival of intestinal allografts in rats. These results suggested that pretreatment with triptolide-modified DC prolonged the survival of rat small bowel allografts after transplantation.
Chen
T
T
The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Xu
H
H
Wang
H Q
HQ
Zhao
Y
Y
Zhu
C F
CF
Zhang
Y H
YH
Ji
M J
MJ
Hua
Y B
YB
Wu
W X
WX
D000818:Animals; D018122:B7-1 Antigen / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000637:transplantation*; D004224:Diterpenes / Q000627:therapeutic use*; D004852:Epoxy Compounds / Q000627:therapeutic use; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007421:Intestine, Small / Q000637:transplantation*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D010616:Phenanthrenes / Q000627:therapeutic use*; D051381:Rats; D011920:Rats, Inbred WF; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology
D018122:B7-1 Antigen; D004224:Diterpenes; D004852:Epoxy Compounds; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D010616:Phenanthrenes; C001899:triptolide