[The initial mechanism's investigation of pilose antler polypeptides resisting replicative senescence of rat chondrocyte].
OBJECTIVE
To investigate the mechanism of pilose antler polypeptides (PAP) resisting replicative senescence of rat chondrocyte serially subcultivated in vitro by means of PAP interfering and controlled experiment.


METHODS
The successive tert-generation (2nd passage, 3rd passage, 4th passage) chondrocytes and the 4th passage cells intervented by PAP were studied for senenscence mechanism. In this course, immunocytochemistry was applied for pl6, pRb, E2F, CyclinD, CDK4 and TRAP-ELISA (telomerase repeat amplification protocol assay-enzyme linked immunosorbent assay) was applied for telomerase activation to observe targets' changing regarding to senescence and the function of PAP.


RESULTS
Along with cell's replicative senescence, pl6, pRb and Cyclin D express significantly rised (P < 0.01), while E2F, CDK4 and telomerase express significantly lowerd (P < 0.01). Meanwhile, in PAP interfered group compared with which in 4th passage group, pl6, pRb and Cyclin D express significantly lowerd (P < 0.01l), while E2F, CDK4 and telomerase express significantly rised (P < 0.01).


CONCLUSIONS
PAP has function that it reversingly affect the express of factors which controlling cell life cycle and cell growth to postpone chondrocyte senenscence.
Chen
Xiao-dong
XD
Shenzhen Pingle Hospital of Orthopedics and Traumatology, Shenzhen 518010,Guang-dong, China. cxdf22@163.com
Lin
Jian-hua
JH
D000818:Animals; D000999:Antlers / Q000737:chemistry*; D016922:Cellular Senescence / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D056741:Cyclin D; D051358:Cyclin-Dependent Kinase 4 / Q000032:analysis; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D016213:Cyclins / Q000032:analysis; D050684:E2F Transcription Factors / Q000032:analysis; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016160:Retinoblastoma Protein / Q000032:analysis
D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D050684:E2F Transcription Factors; D010455:Peptides; D016160:Retinoblastoma Protein; D051358:Cyclin-Dependent Kinase 4