title: [Relationship between epithelial-mesenchymal transition and lung metastasis in hepatocellular carcinoma].
abstract: OBJECTIVE
  To evaluate the relationship between epithelial mesenchymal transition (EMT) and lung metastasis in hepatocellular carcinoma.
  
  
  METHODS
  There were 100 patients who underwent surgical resection of hepatocellular carcinoma between January 2000 and March 2004. They were classified with non-distance metastasis and lung metastasis depend on the close following up till March 2007. Their hepatocellular carcinoma specimens were retrospectively examined for EMT markers (E-cadherin, Vimentin, Fibronectin) with immunochemistry staining in tissue microarray. Univariate and multivariate analysis were used for study the relationship between EMT and lung metastasis.
  
  
  RESULTS
  Univariate analysis showed that down regulation of E-cadherin, overexpression of fibronectin, cytosolic expression of vimentin, AFP >or= 400 ng/ml, tumor size more than 10 cm, portal vein involvement, poorly differentiated of tumor had close correlation with lung metastasis. Multivariate analysis indicated that overexpression of fibronectin was independent factor for lung metastasis apart from tumor size more than 10 cm, portal vein involvement and poorly differentiated of tumor.
  
  
  CONCLUSIONS
  The results proposed that EMT has close relation with lung metastasis in hepatocellular carcinoma.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Tong-chun
authors.initials: TC
authors.lastname: Xue
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Xie
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Sheng-long
authors.initials: SL
authors.lastname: Ye
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Ren
mesh_terms: D000328:Adult; D000368:Aged; D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005500:Follow-Up Studies; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D017154:Stromal Cells / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; D005353:Fibronectins; D014746:Vimentin