Amperometric sensor based on ferrocene-modified multiwalled carbon nanotube nanocomposites as electron mediator for the determination of glucose.
A kind of nanocomposite with good dispersion in water was prepared through covalent adsorption of ferrocenecarboxaldehyde on multiwalled carbon nanotubes (MWNTs) for electrical communication between glucose oxidase (GOD) and electrode. The ferrocene-modified multiwalled carbon nanotube nanocomposites (MWNTs-Fc) could be conveniently cast on electrode surfaces. With the aid of chitosan, GOD was then immobilized on the nanostructure film to form a reagentless amperometric sensor for glucose determination. FTIR spectra and cyclic voltammetry were used to characterize the nanocomposites. The presence of both ferrocene as mediator of electron transfer and MWNTs as conductor enhanced greatly the enzymatic response to the oxidation of glucose. The novel biosensor exhibited a fast response toward glucose with a detection limit of 3.0 x 10(-6) mol/L and the linear range extended up to 3.8 x 10(-3) mol/L.
Qiu
Jian-Ding
JD
Department of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang, PR China. jdqiu@ncu.edu.cn
Zhou
Wen-Mei
WM
Guo
Jin
J
Wang
Rui
R
Liang
Ru-Ping
RP
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D048271:Chitosan; D004566:Electrodes; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D000075163:Metallocenes; D037742:Nanotubes, Carbon / Q000737:chemistry*
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose; C004998:ferrocene