title: Distribution of tenascin-C, fibronectin and collagen types III and IV during regeneration of rat submandibular gland.
abstract: The aim of this study was to determine the localization of tenascin-C, fibronectin and collagen types III and IV during regeneration of the rat submandibular gland. After 7 days' obstruction, the regenerating glands were collected at days 0, 1, 3, 5, 7, 11 and 14 after duct release to study regeneration. Immunohistochemical staining revealed that tenascin-C was strongly expressed in the epithelial cells of duct-acinar structures at days 0-3, and down-regulated in its expression from day 5 to 11, though weak expression was detected in the intercalated duct and acinar cells of the normal gland. Strong labeling of fibronectin was detected around duct-acinar structures during days 0-3 of regeneration. Type IV collagen was expressed strongly in the thickened basement membrane of acinar cells and duct-acinar structures during days 0-3, but weakly around large ducts, though type III collagen was expressed at consistent levels. These findings suggest that tenascin-C and fibronectin affect only the duct-acinar structures, and type IV but not type III collagen is involved in the regeneration of acinar cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ueda
authors.forename: O
authors.initials: O
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Hide
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D024061:Collagen Type III / Q000032:analysis / Q000096:biosynthesis; D024141:Collagen Type IV / Q000032:analysis / Q000096:biosynthesis; D004847:Epithelial Cells / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000096:biosynthesis*; D005353:Fibronectins / Q000096:biosynthesis; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D018987:Salivary Ducts / Q000166:cytology / Q000378:metabolism; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D019063:Tenascin / Q000032:analysis / Q000096:biosynthesis
chemical_list: D024061:Collagen Type III; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019063:Tenascin