title: Isolation and identification of alpha,alpha-trehalose and glycerol from an arctic psychrotolerant Streptomyces sp. SB9 and their possible role in the strain's survival.
abstract: Streptomyces sp. strain SB9 was isolated from perm frost soil samples in Spitsbergen, Arctic Ocean; it grows in a temperature range between 4 degrees C and 28 degrees C. During the survey of biologically active metabolites biosynthesized by this strain, significant amounts of alpha,alpha-trehalose (1) and glycerol (2) were detected. The compounds were isolated from the mycelium, were chromatographically separated, and the structures were elucidated on the basis of MS and NMR measurements. A possible role of trehalose in cold adaptation of the strain was examined. It was determined that the mycelium of the strain cultivated at 4 degrees C accumulated 5-fold higher amounts of trehalose in comparison with the cells cultivated at 28 degrees C. The mesofilic reference strains, Streptomyces spectabilis NRRL 2494 and Streptomyces lividans TK64, accumulated 100-fold less trahalose than the psychrotolerant Streptomyces sp. SB9. High amounts of trehalose in the cells could be a reason for adaptation of the strain to life at Arctic conditions.
authors.affiliation: Bulgarian Academy of Sciences, The Stephan Angeloff Institute of Microbiology, Sofia, Bulgaria. venibiva@microbio.bas.bg
authors.forename: Veneta
authors.initials: V
authors.lastname: Ivanova
authors.forename: Dimitrinka
authors.initials: D
authors.lastname: Lyutskanova
authors.forename: Margarita
authors.initials: M
authors.lastname: Stoilova-Disheva
authors.forename: Mariana
authors.initials: M
authors.lastname: Kolarova
authors.forename: Krasja
authors.initials: K
authors.lastname: Aleksieva
authors.forename: Vesela
authors.initials: V
authors.lastname: Raykovska
authors.forename: Veselka
authors.initials: V
authors.lastname: Peltekova
authors.forename: Hartmut
authors.initials: H
authors.lastname: Laatsch
mesh_terms: D001110:Arctic Regions; D002470:Cell Survival; D005990:Glycerol / Q000302:isolation & purification* / Q000378:metabolism*; D012988:Soil Microbiology; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000378:metabolism*; D014199:Trehalose / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D014199:Trehalose; D005990:Glycerol