title: The in vitro and in ovo responses of chickens to TLR9 subfamily ligands.
abstract: Although Toll-like receptors (TLRs) have been well characterised in mammals, less work has been carried out in non-mammalian species, such as chickens. In this study the response of chicken cells to the TLR9 subfamily of ligands was characterised in vitro and in ovo. It was found that even though chickens appear to have only one functional receptor to represent the TLR9 subfamily, stimulation of chicken splenocytes with TLR7 and TLR9 ligands induced proinflammatory cytokine production and cell proliferation, similar to that observed when the homologous mammalian receptors are stimulated. Furthermore, we demonstrated that the in ovo administration of these TLR ligands elicits a response, such as cytokine production, that can be detected post-hatch. The current knowledge of the action of TLR ligands in mammals, in conjunction with their immunomodulating ability shown in this study, draws attention to their potential use as therapeutic agents for the poultry industry.
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratories, Geelong, Victoria 3220, Australia.
authors.forename: Kristie A
authors.initials: KA
authors.lastname: Jenkins
authors.forename: John W
authors.initials: JW
authors.lastname: Lowenthal
authors.forename: Wayne
authors.initials: W
authors.lastname: Kimpton
authors.forename: Andrew G D
authors.initials: AG
authors.lastname: Bean
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D004269:DNA, Bacterial / Q000494:pharmacology; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000187:drug effects / Q000276:immunology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D008024:Ligands; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051199:Toll-Like Receptor 7 / Q000276:immunology / Q000378:metabolism*; D051217:Toll-Like Receptor 9 / Q000819:agonists / Q000276:immunology / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D015850:Interleukin-6; D008024:Ligands; D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9; D006151:Guanosine; D007371:Interferon-gamma; C068562:loxoribine