Therapeutic effects of gelatin matrix-embedded IL-12 gene-modified macrophages in a mouse model of residual prostate cancer.
We evaluated the potential use of intraoperative gelatin matrix hemostatic sealant (GMHS; FloSeal; Baxter Healthcare) embedded with macrophages (Mphi) transduced with murine interleukin (IL)-12 recombinant adenoviral vector (G/Mphi/AdmIL-12) for prevention of recurrence of prostate cancer following radical prostatectomy. Application of G/Mphi/AdmIL-12 resulted in significant suppression of tumor growth and spontaneous lung metastases, a statistically significant survival advantage of the G/Mphi/AdmIL-12-treated animals, more efficient trafficking of Mphi to lymph nodes draining from the prostate and generation of systemic natural killer cell activity and tumor-specific cytolytic T lymphocyte responses compared to the controls in a preclinical mouse model of residual prostate cancer. Our data recommend this treatment as a novel adjuvant for prevention of local recurrence of prostate cancer following radical prostatectomy.
Tabata
K
K
Scott Department of Urology, Baylor College of Medicine, Houston, TX, USA.
Watanabe
M
M
Naruishi
K
K
Edamura
K
K
Satoh
T
T
Yang
G
G
Abdel Fattah
E
E
Wang
J
J
Goltsov
A
A
Floryk
D
D
Soni
S D
SD
Kadmon
D
D
Thompson
T C
TC
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002465:Cell Movement; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005780:Gelatin; D015316:Genetic Therapy; D006490:Hemostatics / Q000494:pharmacology; D018664:Interleukin-12 / Q000235:genetics* / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000401:mortality / Q000473:pathology / Q000628:therapy*
D006490:Hemostatics; D018664:Interleukin-12; D005780:Gelatin