title: A perspective on the Maillard reaction and the analysis of protein glycation by mass spectrometry: probing the pathogenesis of chronic disease.
abstract: The Maillard reaction, starting from the glycation of protein and progressing to the formation of advanced glycation end-products (AGEs), is implicated in the development of complications of diabetes mellitus, as well as in the pathogenesis of cardiovascular, renal, and neurodegenerative diseases. In this perspective review, we provide an overview on the relevance of the Maillard reaction in the pathogenesis of chronic disease and discuss traditional approaches and recent developments in the analysis of glycated proteins by mass spectrometry. We propose that proteomics approaches, particularly bottom-up proteomics, will play a significant role in analyses of clinical samples leading to the identification of new markers of disease development and progression.
authors.affiliation: Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Ames
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: John W
authors.initials: JW
authors.lastname: Baynes
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002908:Chronic Disease; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006801:Humans; D015416:Maillard Reaction; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods
chemical_list: D017127:Glycation End Products, Advanced; D011506:Proteins