title: High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.
abstract: The direct separation of the enantiomers of four 2-aminomono- or dihydroxycyclopentanecarboxylic acids and four 2-aminodihydroxycyclohexanecarboxylic acids was performed on chiral stationary phases containing macrocyclic glycopeptide antibiotics such as teicoplanin (Astec Chirobiotic T and T2), teicoplanin aglycone (Chirobiotic TAG) or ristocetin A (Chirobiotic R) as chiral selectors. The effects of the nature of organic modifiers, the pH, the mobile phase composition and the structures of the analytes on the separation were investigated. Chirobiotic TAG, and in some cases Chirobiotic T, proved to be the most useful of these columns. The elution sequence was determined in most cases.
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, H-6720 Szeged, Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Berkecz
authors.forename: István
authors.initials: I
authors.lastname: Ilisz
authors.forename: Gabriella
authors.initials: G
authors.lastname: Benedek
authors.forename: Ferenc
authors.initials: F
authors.lastname: Fülöp
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Armstrong
authors.forename: Antal
authors.initials: A
authors.lastname: Péter
mesh_terms: D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry / Q000302:isolation & purification*; D006020:Glycopeptides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012310:Ristocetin / Q000737:chemistry; D013237:Stereoisomerism; D017334:Teicoplanin / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D003509:Cyclohexanecarboxylic Acids; D006020:Glycopeptides; D012310:Ristocetin; C016719:ristocetin A; D017334:Teicoplanin; C063101:teicoplanin aglycone