title: Methylation and deamination of CpGs generate p53-binding sites on a genomic scale.
abstract: The formation of transcription-factor-binding sites is an important evolutionary process. Here, we show that methylation and deamination of CpG dinucleotides generate in vivo p53-binding sites in numerous Alu elements and in non-repetitive DNA in a species-specific manner. In light of this, we propose that the deamination of methylated CpGs constitutes a universal mechanism for de novo generation of various transcription-factor-binding sites in Alus.
authors.affiliation: Department of Computational Molecular Biology, Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany. zemojtel@molgen.mpg.de
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zemojtel
authors.forename: Szymon M
authors.initials: SM
authors.lastname: Kielbasa
authors.forename: Peter F
authors.initials: PF
authors.lastname: Arndt
authors.forename: Ho-Ryun
authors.initials: HR
authors.lastname: Chung
authors.forename: Martin
authors.initials: M
authors.lastname: Vingron
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018899:CpG Islands / Q000502:physiology*; D019175:DNA Methylation; D003641:Deamination; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53