title: Effects of different concentrations of 2,4-D and BAP on somatic embryogenesis induction in saffron (Crocus sativus L.).
abstract: To optimize an in vitro protocol for propagation of saffron through somatic embryogenesis, effects of various concentrations of 2,4-D ( 0, 0.25, 0.5, 1, 2, 4 and 8 mg L(-1)) in combination with BAP (0, 0.25, 0.5, 1, 2, 4 and 8 mg L(-1)) were studied. Surface-sterilized corms were cut transversally into equal portions and the upper or lower parts were used separately as explants. All treatments were maintained in the darkness at 24 +/- 2 degrees C. After 70 days, the first globular embryos were observed and the number of embryos on each explant reached to its maximum 3 months after culture. Statistical analysis showed that there were significant differences between treatments regarding the number of embryos induced on each explant. The most effective treatment was 2.0 mg L(-1) 2,4-D + 1.0 mg L(-1) BAP for both types of explant (inducing 6.5 +/- 1.3 and 35.95 +/- 4.9 embryos on each explant for the upper and lower parts, respectively). The average percentages of explants showing embryogenic response were 33.3 and 93.3% for the upper and the lower part of corm tissue respectively in this treatment. Complementary studies are in progress to optimize maturation and germination stages of these somatic embryos.
authors.affiliation: Department of Biology, Science Faculty, Alzahra University, Tehran, Iran.
authors.forename: Sh
authors.initials: Sh
authors.lastname: Rajabpoor
authors.forename: A V
authors.initials: AV
authors.lastname: Azghandi
authors.forename: A
authors.initials: A
authors.lastname: Saboora
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D001593:Benzyl Compounds / Q000494:pharmacology*; D001901:Botany / Q000379:methods; D027622:Crocus / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D018521:Plant Physiological Phenomena; D018520:Plant Shoots / Q000187:drug effects; D011687:Purines / Q000494:pharmacology*
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C480551:benzylaminopurine