title: Effect of a standardised dietary restriction protocol on multiple laboratory strains of Drosophila melanogaster.
abstract: BACKGROUND
  Outcomes of lifespan studies in model organisms are particularly susceptible to variations in technical procedures. This is especially true of dietary restriction, which is implemented in many different ways among laboratories.
  
  
  RESULTS
  In this study, we have examined the effect of laboratory stock maintenance, genotype differences and microbial infection on the ability of dietary restriction (DR) to extend life in the fruit fly Drosophila melanogaster. None of these factors block the DR effect.
  
  
  CONCLUSIONS
  These data lend support to the idea that nutrient restriction genuinely extends lifespan in flies, and that any mechanistic discoveries made with this model are of potential relevance to the determinants of lifespan in other organisms.
authors.affiliation: UCL Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Grandison
authors.forename: Richard
authors.initials: R
authors.lastname: Wong
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Bass
authors.forename: Linda
authors.initials: L
authors.lastname: Partridge
authors.forename: Matthew D W
authors.initials: MD
authors.lastname: Piper
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D031204:Caloric Restriction; D004032:Diet; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D004435:Eating; D005838:Genotype; D008017:Life Expectancy; D023421:Models, Animal; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline