Effect of trichostatin a on viability and microRNA expression in human pancreatic cancer cell line BxPC-3.
OBJECTIVE
To investigate the influence of trichostatin A (TSA) on inhibition of cell proliferation and induction of apoptosis in human pancreatic cancer cells.


METHODS
MTT-based cytotoxicity assay was used to evaluate the cell viability after treatment with TSA. Cell cycle distribution and apoptosis were examined by means of flow cytometry. Expression of microRNA was determined with microRNA array. Expression of miR-200c and miR-21 was detected by Northern blotting.


RESULTS
TSA significantly inhibited the proliferation of BxPC-3 human pancreatic cancer cells in a time- and dose-dependent manner. BxPC-3 cells treated with TSA were arrested in G0/G1 phase and were characterized by increased apoptotic rate, accompanied by differential expression of microRNAs.


CONCLUSIONS
The results suggest that TSA may activate expression of microRNAs that may act as tumor suppressor in human pancreatic cancer cell line BxPC-3.
Zhang
S
S
Department of Gastroenterology, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. shinengz@hotmail.com
Cai
X
X
Huang
F
F
Zhong
W
W
Yu
Z
Z
D017209:Apoptosis / Q000187:drug effects; D015152:Blotting, Northern; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D035683:MicroRNAs / Q000096:biosynthesis / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics*
D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D035683:MicroRNAs; C012589:trichostatin A