title: Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea.
abstract: Carbapenem-resistant Acinetobacter spp. are being increasingly reported worldwide, including in South Korea, where we examined 144 representative isolates collected in a nationwide hospital survey in 2005. Metallo-beta-lactamases were detected in only 19.4% of isolates, none of which were Acinetobacter baumannii, whereas 74.3% of isolates (mostly A. baumannii) expressed bla(OXA) carbapenemase genes. Among the latter, 47 had bla(OXA-23)-like genes and 56 had upregulated bla(OXA-51)-like variants, including bla(OXA-66), (-83), (-109) and (-115); bla(OXA-115) was a novel variant, detected in two isolates. bla(OXA-72) (bla(OXA-40)-like) was detected in only a single Acinetobacter baylyi isolate, whilst three Acinetobacter calcoaceticus isolates had both bla(VIM-2)-like and bla(OXA-58) genes. Pulsed-field gel electrophoresis (PFGE) suggested the spread of A. baumannii clones with OXA carbapenemases within and between hospitals. In conclusion, the recent increase in imipenem-resistant Acinetobacter spp. from South Korea is mostly due to OXA-type carbapenemases.
authors.affiliation: Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kim
authors.forename: Tae Yeal
authors.initials: TY
authors.lastname: Choi
authors.forename: Soung-Eun
authors.initials: SE
authors.lastname: Cho
authors.forename: Seungok
authors.initials: S
authors.lastname: Lee
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Whang
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: David M
authors.initials: DM
authors.lastname: Livermore
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003428:Cross Infection / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007723:Korea; D001618:beta-Lactamases / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase