Heat-shock-induced oxidative stress and antioxidant response in Aspergillus niger 26.
To extend the knowledge about the relationship between heat shock and oxidative stress in lower eukaryotes, the filamentous fungus Aspergillus niger 26 was chosen as a model system. Here, the response of A. niger cells to heat shock is reported. The temperature treatment significantly increased the levels of reactive oxygen species, superoxide anions (O2), and hydrogen peroxide and the rate of cyanide-resistant respiration as a marker of oxidative stress. Enhanced reactive oxygen species generation coincided with an increase in the content of oxidative damaged protein and in the accumulation of the storage carbohydrates trehalose and glycogen. Thermal survival of the A. niger cells corresponded to a significant increase in the levels of the antioxidant enzymes superoxide dismutase and catalase for all variants. These observations suggest that heat and oxidative stress have a common cellular effect.
Abrashev
Radoslav I
RI
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Academician G. Bonchev, 1113 Sofia, Bulgaria.
Pashova
Svetlana B
SB
Stefanova
Lilyana N
LN
Vassilev
Spassen V
SV
Dolashka-Angelova
Pavlina A
PA
Angelova
Maria B
MB
D000975:Antioxidants / Q000378:metabolism*; D001234:Aspergillus niger / Q000254:growth & development / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D018384:Oxidative Stress; D010101:Oxygen Consumption; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism
D000975:Antioxidants; D013481:Superoxides; D006003:Glycogen; D014199:Trehalose; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase