title: Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice.
abstract: A/duck/Guangxi/53/2002 (DKGX/53) and A/duck/Fujian/01/2002 (DKFJ/01) are H5N1 avian influenza viruses that are lethal in chickens. In mice, however, DKFJ/01 is highly pathogenic, whereas DKGX/53 displays low pathogenicity. In this study, we used reverse genetics to demonstrate that two amino acid residues at positions 30 and 215 of the M1 protein of these two viruses are important determinants for pathogenicity in mice. We thus firstly prove the M1 protein contributes to the virulence of H5N1 viruses in mice, and the amino acid residues shown to attenuate the virulence could be targeted in influenza virus candidates for live vaccine development.
authors.affiliation: Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Fan
authors.forename: Guohua
authors.initials: G
authors.lastname: Deng
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Song
authors.forename: Guobin
authors.initials: G
authors.lastname: Tian
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Suo
authors.forename: Yongping
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Guan
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000835:Animals, Wild; D001208:Asia / Q000453:epidemiology; D001717:Birds; D002645:Chickens; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004372:Ducks; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000453:epidemiology / Q000235:genetics* / Q000635:transmission; D051379:Mice; D011201:Poultry Diseases / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics*; D014774:Virulence; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014763:Viral Matrix Proteins