Down-regulation of adenine nucleotide translocase 3 and its role in camptothecin-induced apoptosis in human hepatoma QGY7703 cells.
Adenine nucleotide translocase (ANT) is known as a core component of the mitochondrial permeability transition pore (MPTP) and a key player in cell death. However, its role in camptothecin (CPT)-induced apoptosis has not been examined. We showed that CPT-induced apoptosis in QGY7703 cells and down-regulated the expression of ANT3. Using ANT3 knock-out and overexpression experiments, we provide further evidence that ANT3 plays a contributive role in CPT-induced apoptosis through induction of MPTP. We speculate that the down-regulation of ANT3 upon stimulation with CPT may be part of the molecular basis underlying the mechanism of acquired resistance to CPT.
Hu
Zhenlin
Z
Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. zhenlinhu@hotmail.com
Guo
Xueqing
X
Yu
Qi
Q
Qiu
Lei
L
Li
Jianzhong
J
Ying
Kang
K
Guo
Cheng
C
Zhang
Junping
J
D033781:Adenine Nucleotide Translocator 3 / Q000235:genetics / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D002166:Camptothecin / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology*; D034622:RNA Interference
D033781:Adenine Nucleotide Translocator 3; D000970:Antineoplastic Agents; D002166:Camptothecin