Establishment of a high content assay for the identification and characterisation of bioactivities in crude bacterial extracts that interfere with the eukaryotic cell cycle.
High content microscopy as a screening tool to identify bioactive agents has provided researchers with the ability to characterise biological activities at the level of single cells. Here, we describe the development and the application of a high content screening assay for the identification and characterisation of cytostatic bioactivities from Myxobacteria extracts. In an automated microscopy assay Sf9 insect cells were visualised utilising the stains bisbenzimide Hoechst 33342, calcein AM, and propidium iodide. Imaging data were processed by the ScanR Analysis-software to determine the ploidy and vitality of each cell and to quantify cell populations. More than 98% of the Sf9 cells were viable and the culture consisted of diploid ( approximately 30%), tetraploid ( approximately 60%), polyploidic (<10%) and apoptotic (<5%) cells. Treatment with the reference substances blasticidin, colchicine, paclitaxel, and cytochalasin D induced changes in ploidy and vitality, which were characteristic for the respective bioactive substance. Furthermore, crude extracts from the chivosazole producing Myxobacterium Sorangium cellulosum So ce56 induced an increase of polyploid cells and a decrease in total cell count, while a mutant producing nearly no chivosazole triggered none of these effects. Purified chivosazole induced the same effects as the wild type extract. Similar effects have been observed for the reference compound cytochalasin D. On the basis of this assay, crude extracts of ten different Myxobacteria cultures were screened. Three extracts exhibited strong cytotoxic activities, further five extracts induced weak changes in the ploidy distribution, and two extracts showed no detectable effect within the assay. Therefore, this robust assay provides the ability to discover and characterise cytotoxic and cytostatic bioactivities in crude bacterial extracts.
Jensen
Nickels A
NA
Department of Proteome and Metabolome Research, Faculty of Biology, Bielefeld University, Bielefeld, Germany.
Gerth
Klaus
K
Grotjohann
Tim
T
Kapp
Dieter
D
Keck
Matthias
M
Niehaus
Karsten
K
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D004396:Coloring Agents / Q000378:metabolism; D045424:Complex Mixtures / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology; D054697:Cytostatic Agents / Q000494:pharmacology*; D055808:Drug Discovery; D005456:Fluorescent Dyes / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018942:Macrolides / Q000494:pharmacology; D008853:Microscopy / Q000379:methods*; D009231:Myxococcales / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D011003:Ploidies; D011760:Pyrrolidinones / Q000494:pharmacology
D004396:Coloring Agents; D045424:Complex Mixtures; D054697:Cytostatic Agents; D005456:Fluorescent Dyes; D018942:Macrolides; D011760:Pyrrolidinones; C420726:blasticidin A; C096623:chivosazol A; D015638:Cytochalasin D; D017239:Paclitaxel; D003078:Colchicine