Analyzing P-bodies in Saccharomyces cerevisiae.
Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.
Nissan
Tracy
T
The University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA.
Parker
Roy
R
D000818:Animals; D015415:Biomarkers; D003594:Cytoplasmic Granules / Q000378:metabolism*; D018628:Gene Dosage / Q000235:genetics; D006801:Humans; D008853:Microscopy / Q000379:methods*; D009154:Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D015415:Biomarkers; D029701:Saccharomyces cerevisiae Proteins