Gibberellin precursor is involved in spore germination in the moss Physcomitrella patens.
Gibberellins are ent-kaurene derived phytohormones that are involved in seed germination, stem elongation, and flower induction in seed plants, as well as in antheridia formation and spore germination in ferns. Although ubiquitous in vascular plants, the occurrence and potential function(s) of gibberellins in bryophytes have not yet been resolved. To determine the potential role of gibberellin and/or gibberellin-like compounds in mosses, the effect of AMO-1618 on spores of Physcomitrella patens (Hedw.) B.S.G. was tested. AMO-1618, which inhibited ent-kaurene and gibberellin biosynthesis in angiosperms, also inhibited the bifunctional copalyl diphosphate synthase (E.C. 5.5.1.13)/ent-kaurene synthase (E.C. 4.2.3.19) of P. patens. AMO-1618 also caused a decrease in spore germination rates of P. patens, and this inhibitory effect was less pronounced in the presence of ent-kaurene. These results suggest that ent-kaurene biosynthesis is required by P. patens spores to germinate, implying the presence of gibberellin-like phytohormones in mosses.
Anterola
Aldwin
A
Department of Plant Biology, Southern Illinois University, Carbondale, IL 62901, USA. anterola@siu.edu
Shanle
Erin
E
Mansouri
Katayoun
K
Schuette
Scott
S
Renzaglia
Karen
K
D019883:Alkyl and Aryl Transferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D019068:Bryopsida / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D045786:Diterpenes, Kaurane / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D005875:Gibberellins / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism
D045786:Diterpenes, Kaurane; D005875:Gibberellins; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; C006155:AMO-1618; D019883:Alkyl and Aryl Transferases; C090963:ent-kaurene synthetase A; C105712:ent-kaurene synthetase B