title: In vitro differentiation of human cord blood-derived unrestricted somatic stem cells into hepatocyte-like cells on poly(epsilon-caprolactone) nanofiber scaffolds.
abstract: Tissue engineering of implantable cellular constructs is an emerging cellular therapy for hepatic disease. In this study, we tested the ability of poly(epsilon-caprolactone) (PCL) nanofiber scaffold to support and maintain hepatic differentiation of human cord blood-derived unrestricted somatic stem cells (USSCs) in vitro. USSCs, self-renewing pluripotent cells, were isolated from human cord blood. The electrospun PCL nanofiber porous scaffold was constructed of uniform, randomly oriented nanofibers. USSCs were seeded onto PCL nanofiber scaffolds, and were induced to differentiate into hepatogenic lineages by culturing with differentiation factors for 6 weeks. RT-PCR analysis of endoderm and hepatic-specific gene expression, immunohistochemical detection of cytokeratin 18 (CK-18), alpha-fetoprotein, albumin, glycogen storage and indocyanine green uptake confirmed the differentiation of USSCs into endoderm and hepatocyte-like cells. In the present study, we show that hepatocyte-like cells differentiated from USSCs on the PCL nanofiber scaffold can be candidate for tissue engineering and cell therapy of hepatic tissues.
authors.affiliation: Department of Stem Cell and Tissue Engineering, Stem Cell Technology Co. Ltd., Tehran, Iran.
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Hashemi
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Seyed Shahrooz
authors.initials: SS
authors.lastname: Zargarian
authors.forename: Vahid
authors.initials: V
authors.lastname: Haddadi-Asl
authors.forename: Naser
authors.initials: N
authors.lastname: Ahmadbeigi
authors.forename: Sara
authors.initials: S
authors.lastname: Soudi
authors.forename: Yousof
authors.initials: Y
authors.lastname: Gheisari
authors.forename: Athena
authors.initials: A
authors.lastname: Hajarizadeh
authors.forename: Yousef
authors.initials: Y
authors.lastname: Mohammadi
mesh_terms: D002208:Caproates / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004867:Equipment Design; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression; D006003:Glycogen / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007208:Indocyanine Green / Q000378:metabolism; D007783:Lactones / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002208:Caproates; D007783:Lactones; D011108:Polymers; C121056:caprolactone; D006003:Glycogen; D007208:Indocyanine Green