[Study on fingerprint spectrum of Lonicera macranthoides by HPLC].
OBJECTIVE
To establish the fingerprint spectrum of Lonicera macranthoides by HPLC.


METHODS
The column of Hypersil gold C18 (4. 6 mm x 250 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.05% phosphoric acid with gradient elution. The column temperature was 30 degrees C, the detective wavelength was 240 nm, and the flow rate was 0.5 ml/min.


RESULTS
Fingerprint spectrum of Lonicera macranthoides was established, and 11 samples of different origin Lonicera macranthoides were detected. Ten peaks in the chromatogram were common by Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A). There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.


CONCLUSIONS
This method is accurate, reliable and provides a scientific basis for controlling the quality of Lonicera macranthoides.
Xin
Ning
N
Guangxi Traditional Chinese Medical University, Nanning 530001, China. xn@gxtcmu.edu.cn
Feng
Jie
J
Huang
Lu-liang
LL
Yin
Sheng-gao
SG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D035264:Flowers / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry* / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal