Association of extracellular superoxide dismutase (SOD3) Ala40Thr gene polymorphism with pre-eclampsia complicated by severe fetal growth restriction.
OBJECTIVE
Placental and systemic oxidative stress with an imbalance in the oxidant/antioxidant activity seems to play a central role in the pathogenesis of pre-eclampsia. The aim of our study was to examine whether two missense polymorphisms of the extracellular superoxide dismutase (SOD3) gene (Arg213Gly and Ala40Thr) are associated with pre-eclampsia in a Caucasian population from Hungary.


METHODS
One hundred and fifty-nine pre-eclamptic patients and 114 normotensive, healthy pregnant women were involved in this case-control study. The SOD3 Arg213Gly and Ala40Thr genotypes were determined using the polymerase chain reaction-restriction length polymorphism (PCR-RFLP) and allele-specific amplification methods.


RESULTS
The Arg213Gly variant was not detected in our population. There were no significant differences in the genotype and allele frequencies of the SOD3 Ala40Thr polymorphism between pre-eclamptic patients and control subjects. However, the mutant allele carriers of this polymorphism showed an increased risk for severe fetal growth restriction-complicated pre-eclampsia, which was independent of maternal age, prepregnancy BMI, primiparity and smoking status (OR: 6.07, 95% CI: 1.33-27.8, p=0.020; adjusted OR: 4.89, 95% CI: 1.03-23.2, p=0.046).


CONCLUSIONS
Our results suggest a role of SOD3 Ala40Thr single nucleotide polymorphism in the risk of severe fetal growth restriction-complicated pre-eclampsia. However, further studies are needed with a larger sample size to confirm our findings and to explore the exact molecular basis of this observation.
Rosta
Klára
K
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary.
Molvarec
Attila
A
Enzsöly
Anna
A
Nagy
Bálint
B
Rónai
Zsolt
Z
Fekete
Andrea
A
Sasvári-Székely
Mária
M
Rigó
János
J
Vér
Agota
A
D000328:Adult; D019943:Amino Acid Substitution; D016022:Case-Control Studies; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology / Q000235:genetics*; D005838:Genotype; D006801:Humans; D006814:Hungary; D020125:Mutation, Missense; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011225:Pre-Eclampsia / Q000201:enzymology / Q000235:genetics*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000235:genetics*; D055815:Young Adult
C441642:SOD3 protein, human; D013482:Superoxide Dismutase