Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry.
Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 microg/mL for glucuronides of androsterone and 11beta-OHAn-3G, and 5-500 ng/mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis.
Cho
Sung-Hee
SH
Bioanalysis and Biotransformation Research Center, KIST, Seoul 136-791, Korea.
Lee
Jeongae
J
Choi
Man Ho
MH
Lee
Won-Yong
WY
Chung
Bong Chul
BC
D000728:Androgens / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D020719:Glucuronides / Q000652:urine*; D006801:Humans; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000728:Androgens; D020719:Glucuronides