title: Synthesis of a Streptococcus pyogenes vaccine candidate based on the M protein PL1 epitope.
abstract: Group A streptococcus is a Gram-positive bacteria that causes a range of infectious diseases. Targeting the bacteria, a new self-adjuvanting vaccine candidate, incorporating a carbohydrate carrier and an amino acid-based adjuvant, was synthesised utilising carbohydrate chemistry and solid-phase peptide synthesis procedures. Characterisation of the candidate was achieved using reverse-phase HPLC and electrospray ionisation mass spectrometry. The successful synthesis and characterisation of the vaccine candidate may contribute to the discovery of a therapeutically and clinically viable vaccine against group A streptococcus.
authors.affiliation: The University of Queensland, School of Molecular and Microbial Sciences, Chemistry Building 68, St. Lucia, Brisbane, Qld 4072, Australia. p.simerska@uq.edu.au
authors.forename: Pavla
authors.initials: P
authors.lastname: Simerska
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D000942:Antigens, Bacterial / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008956:Models, Chemical; D021241:Spectrometry, Mass, Electrospray Ionization; D022541:Streptococcal Vaccines / Q000276:immunology*; D013291:Streptococcus / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000276:immunology*
chemical_list: D000596:Amino Acids; D000941:Antigens; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002241:Carbohydrates; D000939:Epitopes; D008055:Lipids; D022541:Streptococcal Vaccines