Protective immunity of recombinant Mycobacterium bovis BCG expressing rhomboid gene against Eimeria tenella challenge.
Two recombinant Mycobacterium bovis BCG (rBCG) strains carrying the Eimeria tenella rhomboid gene (Rho) delivered by extrachromosomal vector pMV261 and integrative vector pMV361 were evaluated for their ability to protect chickens against E. tenella challenge. The chickens were immunized intranasal with BCG, rBCG pMV261-Rho, or rBCG pMV361-Rho twice at a 2-week interval. All the recombinant BCG immunized chickens developed specific immune responses, and there was a significant increases of the percentages of CD4(+) and CD8(+) cells compared to the control (P<0.05). Challenge experiments demonstrated that the two rBCG strains could provide significant protection against E. tenella challenge. But vaccination with rBCG pMV261-Rho induced higher specific antibody titers and produced greater protection rate (56.04%) than rBCG pMV361-Rho group (P<0.05). These results indicated that M. bovis BCG is a novel vaccine vector to express and present antigens of E. tenella, and rBCG has a potential as vaccine in chickens.
Wang
Qiuyue
Q
College of Animal Science and Veterinary Medicine, JiLin University, 5333 Xi'an Road, Changchun 130062, China.
Li
Jianhua
J
Zhang
Xichen
X
Liu
Quan
Q
Liu
Chengwu
C
Ma
Guangpeng
G
Cao
Lili
L
Gong
Pengtao
P
Cai
Yanan
Y
Zhang
Guocai
G
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D001500:BCG Vaccine / Q000276:immunology*; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002645:Chickens; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics; D007111:Immunity, Cellular / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count / Q000662:veterinary; D009163:Mycobacterium bovis / Q000235:genetics* / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology
D000913:Antibodies, Protozoan; D001500:BCG Vaccine; D014614:Vaccines, Synthetic