title: [Curcumin inhibiting the expression of indoleamine 2,3-dioxygenase induced by IFN-gamma in cancer cells].
abstract: OBJECTIVE
  To investigate the effect of curcumin on indoleamine 2, 3 -dioxygenase (IDO) expression induced by IFN-gamma in cancer cells.
  
  
  METHODS
  A431, HeLa, HepG2, CNE2 cells were treated with curcumin for 24 hours, then the cell proliferation was detected by methyl thiazolyl tetrazolium (MTF) assay. The effects of curcumin on IDO expression induced by IFN-gamma in these cancer cells were demonstrated by Western blot. The transcription of interferon responsive factor-1 (IRF-1), which was a key transcription factor regulating IDO expression, was analyzed by reverse transcription polymerase chain reaction (RT-PCR) under the treatment of curcumin.
  
  
  RESULTS
  Curcumin inhibited the expression of IDO in these cancer cells. However, curcumin did not inhibit the transcription of IRF-1 in cancer cells.
  
  
  CONCLUSIONS
  Curcumin could inhibit the expression of IDO in cancer cells.
authors.affiliation: Department of Pharmacy, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Kun-shui
authors.initials: KS
authors.lastname: Zhang
authors.forename: Guo-cheng
authors.initials: GC
authors.lastname: Li
authors.forename: Yu-wen
authors.initials: YW
authors.lastname: He
authors.forename: Yan-mei
authors.initials: YM
authors.lastname: Yi
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: Liao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Du
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism*; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004791:Enzyme Inhibitors; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D050856:Interferon Regulatory Factor-1; D007371:Interferon-gamma; D003474:Curcumin