Characterization of a Vitellogenin Gene Fragment in Boophilus microplus Ticks.
The objective of this study was to isolate, clone, and characterize a fragment of the vitellogenin (Vg) gene from a B. microplus tick strain from Mexico. Using cDNA and specific primers, an 1800-bp fragment was amplified, cloned, and transformed in into E. coli, and then sequenced. Comparative analysis with a previously reported sequence showed 99% identity at both the nucleotide and amino acid level. The predicted amino acid sequence of the Mexican Vg has 6 positive mutations. There is an insertion of an aspartic acid on position 26 and a deletion on position 552 with respect to the reported sequence. There were 11 predicted glycosylation sites conserved in both strains. It is concluded that there is a high sequence homology of Vg in both strains.
Granjeno-Colin
Gabriela
G
FMVZ, Universidad Nacional Autonoma de México, D.F. México, México.
Hernandez-Ortiz
Ruben
R
Mosqueda
Juan
J
Estrada-Mondaca
Sandino
S
Figueroa
Julio V
JV
Garcia-Vazquez
Zeferino
Z
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006031:Glycosylation; D009154:Mutation; D016133:Polymerase Chain Reaction; D013987:Ticks / Q000235:genetics*; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D014819:Vitellogenins