title: Vaginal fluid human chorionic gonadotropin for detection of rupture of membranes.
abstract: OBJECTIVE
  To evaluate the accuracy of qualitative and quantitative human chorionic gonadotropin (hCG) assays to detect ruptured amniotic membranes (ROM) in term patients.
  
  
  METHODS
  We conducted a prospective cohort study enrolling 100 term pregnancies with intact membranes. Samples were collected before and immediately following ROM, and at 2 and 4 hours post-ROM. Quantitative and qualitative hCG assays were performed on all samples. Outcome measures included the detection and measured amount of hCG before and after ROM. Sensitivity, specificity and positive predictive values for the qualitative assays were determined.
  
  
  RESULTS
  A total of 98% pre-ROM and 100% post-ROM qualitative assays tested positive with mean quantitative hCG levels of 181.30 mIU/mL and 208.24 mIU/mL, respectively. The qualitative post-ROM sensitivity was 100%; however, the pre-ROM specificity was only 2%. The positive predictive value for detecting ROM was 50%.
  
  
  CONCLUSIONS
  Qualitative and quantitative hCG assays are not useful for determining ROM in term pregnancies.
authors.affiliation: Department of Obstetrics and Gynecology, Wilford Hall Medical Center, Lackland Air Force Base, Texas, USA. rd-beesley@yahoo.com
authors.forename: Ronald
authors.initials: R
authors.lastname: Beesley
authors.forename: Naomi
authors.initials: N
authors.lastname: Trevino
authors.forename: Michael
authors.initials: M
authors.lastname: Smyth
authors.forename: Claire
authors.initials: C
authors.lastname: Gould
authors.forename: Randal
authors.initials: R
authors.lastname: Robinson
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D006063:Chorionic Gonadotropin / Q000032:analysis*; D015331:Cohort Studies; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis*; D006801:Humans; D011237:Predictive Value of Tests; D011247:Pregnancy; D014621:Vagina / Q000737:chemistry*
chemical_list: D006063:Chorionic Gonadotropin