title: Triphala incorporated collagen sponge--a smart biomaterial for infected dermal wound healing.
abstract: BACKGROUND
  Wound infection is a major problem in the medical community since many types of wounds are more prone to microbial contamination leading to infection. Triphala (a traditional ayurvedic herbal formulation) incorporated collagen sponge was investigated for its healing potential on infected dermal wound in albino rats.
  
  
  METHODS
  Methanol extract of triphala was prepared and analyzed for the presence of catechin by high-pressure liquid chromatography analysis. Collagen sponge was prepared by incorporating triphala into collagen sponge. The triphala incorporated collagen was characterized by Fourier transform infrared spectroscopy, differential scanning calorimetry, and water uptake analysis. Infected wound was dressed with triphala incorporated collagen sponge. Wound reduction rate, collagen content, and matrix metalloproteinases in the granulation tissue, histology, and Fourier transform electron microscopy analysis were done to obtain the healing pattern.
  
  
  RESULTS
  High-pressure liquid chromatography analysis showed the presence of (-)epigallocatechin gallate. FT-IR spectroscopy study revealed the interaction of polyphenols with the collagen. Triphala incorporated collagen sponge has shown to increase thermal stability and water uptake capability, faster wound closure, improved tissue regeneration, collagen content at the wound site, and supporting histopathological parameters pertaining to wound healing. Matrix metalloproteinases expression was correlated well with reduction in the inflammatory phase, thus confirming efficacy of the dressing.
  
  
  CONCLUSIONS
  Better healing efficacy of triphala incorporated collagen sponge may provides a scientific rationale for the use of this dressing as an effective wound cover in the management of infected dermal wound.
authors.affiliation: Bio-Products Laboratory, Central Leather Research Institute, Chennai, India.
authors.forename: Muthusamy Senthil
authors.initials: MS
authors.lastname: Kumar
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Kirubanandan
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Sripriya
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Sehgal
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials; D002152:Calorimetry, Differential Scanning; D002392:Catechin / Q000032:analysis; D003094:Collagen / Q000008:administration & dosage*; D007150:Immunohistochemistry; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D010936:Plant Extracts / Q000008:administration & dosage*; D011161:Porifera; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D001672:Biocompatible Materials; D010936:Plant Extracts; C520904:triphala; D002392:Catechin; D003094:Collagen; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9