[7-aminoactinomycin as a fluorescent probe for DNA unwinding and denaturation].
A fluorescent analogue of the antibiotic actinomycin D, 7-aminoactinomycin D (7AAMD), which is widely used in molecular biology, was shown by steady-state, polarization, and phase fluorescent spectroscopy to bind primarily in unwound regions of DNA with a concomitant increase in its emission intensity. The maximum emission intensity of 7AAMD is observed for denatured DNA. Thus, 7AAMD may serve as a good indicator of DNA unwinding, denaturation, and fragmentation.
Bitekhtina
M A
MA
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia.
Vekshin
N L
NL
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D053938:DNA Fragmentation; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation
D005456:Fluorescent Dyes; D003609:Dactinomycin; C025942:7-aminoactinomycin D; D004247:DNA