title: Monitoring patterns of gene expression in Dictyostelium by beta-galacotsidase staining.
abstract: Monitoring the spatial distribution of prespore and pstB cell types is a sensitive method to monitor GSK-3 and Aar activity during Dictyostelium development. Cell-specific expression of lacZ marker genes can be readily detected using enzymatic cleavage of the substrate X-gal. This protocol describes a simple method for beta-galactosidase staining in developed Dictyostelium structures.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK.
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Harwood
mesh_terms: D000818:Animals; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase