title: The fate of the duplicated androgen receptor in fishes: a late neofunctionalization event?
abstract: BACKGROUND
  Based on the observation of an increased number of paralogous genes in teleost fishes compared with other vertebrates and on the conserved synteny between duplicated copies, it has been shown that a whole genome duplication (WGD) occurred during the evolution of Actinopterygian fish. Comparative phylogenetic dating of this duplication event suggests that it occurred early on, specifically in teleosts. It has been proposed that this event might have facilitated the evolutionary radiation and the phenotypic diversification of the teleost fish, notably by allowing the sub- or neo-functionalization of many duplicated genes.
  
  
  RESULTS
  In this paper, we studied in a wide range of Actinopterygians the duplication and fate of the androgen receptor (AR, NR3C4), a nuclear receptor known to play a key role in sex-determination in vertebrates. The pattern of AR gene duplication is consistent with an early WGD event: it has been duplicated into two genes AR-A and AR-B after the split of the Acipenseriformes from the lineage leading to teleost fish but before the divergence of Osteoglossiformes. Genomic and syntenic analyses in addition to lack of PCR amplification show that one of the duplicated copies, AR-B, was lost in several basal Clupeocephala such as Cypriniformes (including the model species zebrafish), Siluriformes, Characiformes and Salmoniformes. Interestingly, we also found that, in basal teleost fish (Osteoglossiformes and Anguilliformes), the two copies remain very similar, whereas, specifically in Percomorphs, one of the copies, AR-B, has accumulated substitutions in both the ligand binding domain (LBD) and the DNA binding domain (DBD).
  
  
  CONCLUSIONS
  The comparison of the mutations present in these divergent AR-B with those known in human to be implicated in complete, partial or mild androgen insensitivity syndrome suggests that the existence of two distinct AR duplicates may be correlated to specific functional differences that may be connected to the well-known plasticity of sex determination in fish. This suggests that three specific events have shaped the present diversity of ARs in Actinopterygians: (i) early WGD, (ii) parallel loss of one duplicate in several lineages and (iii) putative neofunctionalization of the same duplicate in percomorphs, which occurred a long time after the WGD.
authors.affiliation: INRA-SCRIBE IFR 140, Campus de Beaulieu, 35042 Rennes Cedex, France. douardve@umdnj.edu
authors.forename: Véronique
authors.initials: V
authors.lastname: Douard
authors.forename: Frédéric
authors.initials: F
authors.lastname: Brunet
authors.forename: Bastien
authors.initials: B
authors.lastname: Boussau
authors.forename: Isabelle
authors.initials: I
authors.lastname: Ahrens-Fath
authors.forename: Virginie
authors.initials: V
authors.lastname: Vlaeminck-Guillem
authors.forename: Bernard
authors.initials: B
authors.lastname: Haendler
authors.forename: Vincent
authors.initials: V
authors.lastname: Laudet
authors.forename: Yann
authors.initials: Y
authors.lastname: Guiguen
mesh_terms: D013734:Androgen-Insensitivity Syndrome / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D020440:Gene Duplication; D016678:Genome; D006801:Humans; D016013:Likelihood Functions; D008297:Male; D009154:Mutation; D010802:Phylogeny; D011944:Receptors, Androgen / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: C410798:AR protein, human; D029941:Fish Proteins; D011944:Receptors, Androgen