title: Marchantin C, a novel microtubule inhibitor from liverwort with anti-tumor activity both in vivo and in vitro.
abstract: Microtubules are long-standing targets in cancer chemotherapy. Previously, we reported that marchantin C triggers apoptosis of human tumor cells. We show here that marchantin C induced cell cycle arrest at G(2)/M phase in A172 and HeLa cells. In addition, marchantin C decreased the quantity of microtubules in a time- and dose-dependent manner in these cells. Exposure of purified bovine brain tubulin to marchantin C inhibited polymerization of gross tubulin in vitro. Moreover, marchantin C potently suppressed the growth of human cervical carcinoma xenografts in nude mice. Marchantin C-treated xenografts showed decreased microtubules, Bcl-2 and increased cyclin B1, Bax, caspase-3, indicating that marchantin C possess the same ability to induce microtubules depolymerization and tumor cell apoptosis in tumor-bearing mice as in vitro. In conclusion, marchantin C is a novel microtubule inhibitor that induces mitotic arrest of tumor cells and suppresses tumor cell growth, exhibiting promising antitumor therapeutic potential.
authors.affiliation: School of Pharmaceutical Sciences, 44 Wen Hua Xi Road, Jinan, Shandong, China.
authors.forename: Yan-qiu
authors.initials: YQ
authors.lastname: Shi
authors.forename: Chang-jun
authors.initials: CJ
authors.lastname: Zhu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Yuan
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Xian-jun
authors.initials: XJ
authors.lastname: Qu
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Yan-na
authors.initials: YN
authors.lastname: Cheng
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Lou
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001632:Bibenzyls / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005260:Female; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism; D010647:Phenyl Ethers / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001632:Bibenzyls; D002396:Catechols; D004988:Ethers, Cyclic; D010647:Phenyl Ethers; C459129:marchantin C