The nuclear orphan receptor COUP-TFII plays an essential role in adipogenesis, glucose homeostasis, and energy metabolism.
Adipose tissue development and function play a central role in the pathogenesis and pathophysiology of metabolic syndromes. Here, we show that chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) plays a pivotal role in adipogenesis and energy homeostasis. COUP-TFII is expressed in the early stages of white adipocyte development. COUP-TFII heterozygous mice (COUP-TFII(+/-)) have much less white adipose tissue (WAT) than wild-type mice (COUP-TFII(+/+)). COUP-TFII(+/-) mice display a decreased expression of key regulators for WAT development. Knockdown COUP-TFII in 3T3-L1 cells resulted in an increased expression of Wnt10b, while chromatin immunoprecipitation analysis revealed that Wnt10b is a direct target of COUP-TFII. Moreover, COUP-TFII(+/-) mice have increased mitochondrial biogenesis in WAT, and COUP-TFII(+/-) mice have improved glucose homeostasis and increased energy expenditure. Thus, COUP-TFII regulates adipogenesis by regulating the key molecules in adipocyte development and can serve as a target for regulating energy metabolism.
Li
Luoping
L
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Xie
Xin
X
Qin
Jun
J
Jeha
George S
GS
Saha
Pradip K
PK
Yan
Jun
J
Haueter
Claire M
CM
Chan
Lawrence
L
Tsai
Sophia Y
SY
Tsai
Ming-Jer
MJ
D041721:3T3-L1 Cells; D050156:Adipogenesis; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D051837:COUP Transcription Factor II / Q000378:metabolism*; D002454:Cell Differentiation; D002645:Chickens; D004734:Energy Metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism*; D006579:Heterozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000209:etiology / Q000517:prevention & control; D013997:Time Factors; D051153:Wnt Proteins / Q000378:metabolism
D051837:COUP Transcription Factor II; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse; D005947:Glucose