title: N-glycosylation pattern of E2 glycoprotein from classical swine fever virus.
abstract: The extracellular domain of E2 glycoprotein outer surface of the classical swine fever virus was expressed in epithelial kidney pig cells. The N-glycosylation determined by combination of Normal Phase-HPLC, Weak Anion Exchange-HPLC, exoglycosidase digestions and Mass Spectrometry revealed a complex mixture of neutral and monosialylated multiantennary N-glycans with variable number of alpha1-3-Gal-Gal antennae terminals. The most abundant neutral N-glycan has a composition of Hex(7)HexNAc(4)dHex(1), Negative ion ESI-MS/MS confirmed the presence of the alpha1-3-Gal-Gal motif on each arm of the fucosylated biantennary N-glycan. The most abundant monosialylated glycan was Hex(6)HexNAc(4)dHex(1)Neu(5)Ac(1), with the sialic acid linked to the terminal beta1-4-Gal-GlcNAc. Sialic acid on the antenna capping position was predominantly of the N-acetyl form.
authors.affiliation: Department of Carbohydrate Chemistry, Center for Genetic Engineering and Biotechnology, Havana 10600, Cuba.
authors.forename: Raquel
authors.initials: R
authors.lastname: Montesino
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Toledo
authors.forename: Oliberto
authors.initials: O
authors.lastname: Sanchez
authors.forename: Yasser
authors.initials: Y
authors.lastname: Zamora
authors.forename: Maritza
authors.initials: M
authors.lastname: Barrera
authors.forename: Louise
authors.initials: L
authors.lastname: Royle
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Dwek
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: Jose A
authors.initials: JA
authors.lastname: Cremata
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D007668:Kidney / Q000166:cytology; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis*; D034421:Sus scrofa; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus; D006026:Glycoside Hydrolases