title: [Analyses of the expressions of GFAP in the brain tissues of hamsters infected with various amounts of scrapie strain 263K at terminal stage].
abstract: OBJECTIVE
  To investigate whether gliosis in the brain tissues of the hamsters infected with various amounts of scrapie strain 263K is correlated with the inoculation doses or the incubation times.
  
  
  METHODS
  The total values of glial fibrillary acidic protein (GFAP) in brains were evaluated by Western Blots and the GFAP-stained cells were detected by immunohistochemistry (IHC). The characteristics of GFAP distributions among various groups were defined by quantitive and statistic analyses.
  
  
  RESULTS
  Compared with the brain tissues of normal hamsters, remarkably higher total GFAP levels and more GFAP-stained cells were observed in the brain tissues of infected ones, howbeit, no significant difference was addressed among the infected groups.
  
  
  CONCLUSIONS
  Inoculations of various amounts of scrapie strain 263K into experimental hamsters intracerebrally induced the similar patterns of gliosis in the brains at the clinically terminal stage, regardless of infectious doses and incubation times.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Tian
authors.forename: Bao-Yun
authors.initials: BY
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D006224:Cricetinae; D015870:Gene Expression; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism*; D005911:Gliosis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018620:PrPSc Proteins / Q000378:metabolism*; D017096:Prion Diseases / Q000378:metabolism* / Q000473:pathology
chemical_list: D005904:Glial Fibrillary Acidic Protein; D018620:PrPSc Proteins