title: HY253, a novel compound isolated from Aralia continentalis, induces apoptosis via cytochrome c-mediated intrinsic pathway in HeLa cells.
abstract: This study was aimed to elucidate the novel structure of HY253 isolated from the roots of Aralia continentalis and to evaluate its detailed mechanisms on apoptotic induction in HY253-treated HeLa cells. The structure of HY253 was elucidated based on the interpretation of the NMR spectra, as 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol. The TUNEL assay using flow cytometer revealed an appreciable apoptotic induction in HeLa cells treated with 100 microM of HY253 for 48 h. This apoptotic induction is associated with cytochrome c release from mitochondria, via up-regulation of pro-apoptotic Bcl-2 proteins, such as Bax and Bak, which, in turn, resulted in the activation of caspase-8, -9 and -3, and the cleavage of poly(ADP-ribose) polymerase (PARP).
authors.affiliation: Department of Medical Genetics, College of Medicine, Hanyang University, 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Ha Lim
authors.initials: HL
authors.lastname: Oh
authors.forename: Haeyoung
authors.initials: H
authors.lastname: Lim
authors.forename: Younghee
authors.initials: Y
authors.lastname: Park
authors.forename: Yoongho
authors.initials: Y
authors.lastname: Lim
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Koh
authors.forename: Youl-Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: Chul-Hoon
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis; D027983:Aralia / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D015195:Drug Design; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation; D005449:Fluorenes / Q000138:chemical synthesis* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D009682:Magnetic Resonance Spectroscopy; D008928:Mitochondria / Q000378:metabolism; D008956:Models, Chemical; D010936:Plant Extracts / Q000138:chemical synthesis* / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism
chemical_list: C539892:7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol; D000970:Antineoplastic Agents; D005449:Fluorenes; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D045304:Cytochromes c