Pyrenebutyrate-mediated delivery of quantum dots across the plasma membrane of living cells.
Quantum dots have been delivered directly across the plasma membrane to the cytosol of living cells using a combination of a cationic peptide, polyarginine, and a hydrophobic counterion, pyrenebutyrate. Quantum dot delivery did not disrupt the plasma membrane and bypassed the barrier of endocytic vesicles. Cellular uptake was independent of temperature but highly dependent on the surface charge of the quantum dot and the membrane potential of the cell, suggesting a direct translocation across the membrane. This method of delivery can find immediate application for quantum dots and may be broadly applicable to other nanoparticles.
Jablonski
Amy E
AE
School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA.
Humphries
William H
WH
Payne
Christine K
CK
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D002470:Cell Survival; D000882:Haplorhini; D008564:Membrane Potentials; D011721:Pyrenes / Q000737:chemistry*; D045663:Quantum Dots
D011721:Pyrenes; C002218:1-pyrenebutyrate