title: Cross-priming amplification for rapid detection of Mycobacterium tuberculosis in sputum specimens.
abstract: Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear- and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.
authors.affiliation: Department of TB Control, Shanghai Municipal Centers for Disease Control and Prevention, Shanghai 200336, China.
authors.forename: Rendong
authors.initials: R
authors.lastname: Fang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Hu
authors.forename: Qimin
authors.initials: Q
authors.lastname: You
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Huayan
authors.initials: H
authors.lastname: Zhong
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Jian
authors.initials: J
authors.lastname: Mei
authors.forename: Qian
authors.initials: Q
authors.lastname: Gao
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D017931:DNA Primers