title: [Diagnostic value of SYT-SSX fusion gene detection by fluorescence in-situ hybridization for synovial sarcoma].
abstract: OBJECTIVE
  To establish a method of SYT-SSX fusion gene detection by FISH and to explore its diagnostic value for synovial sarcoma.
  
  
  METHODS
  The presence of SYT-SSX fusion gene was determined by FISH using a tissue microarray containing 62 known synovial sarcomas, 60 non-synovial sarcomas and 133 equivocal synovial sarcomas. FISH results were compared with those of RT-PCR published previously.
  
  
  RESULTS
  Overall, 96.9% (247/255) of the cases were successfully analyzed by FISH. The sensitivity of FISH for known synovial sarcomas was 96.7% (58/60), and the specificity for the non-synovial sarcoma was 100% (59/59). Moreover, SYT-SSX gene fusion was detected in 78.1% (100/128) of the equivocal synovial sarcomas. The concordance rate between FISH and RT-PCR was 83.6% (102/122) in those equivocal synovial sarcomas, and overall 79.7% (106/133) of these cases were confirmed as synovial sarcomas either by RT-PCR or by FISH.
  
  
  CONCLUSIONS
  The sensitivity and specificity of FISH detection of SYT-SSX fusion gene are high. FISH and RT-PCR are complementary to each other in the confirmation of synovial sarcomas, particularly those questionable cases.
authors.affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin 300060, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Bao-Cun
authors.initials: BC
authors.lastname: Sun
authors.forename: Yan-Xue
authors.initials: YX
authors.lastname: Liu
authors.forename: Shi-Wu
authors.initials: SW
authors.lastname: Zhang
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Xi-Shan
authors.initials: XS
authors.lastname: Hao
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000302:isolation & purification*; D057089:Pathology, Molecular / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013584:Sarcoma, Synovial / Q000175:diagnosis* / Q000235:genetics; D012983:Soft Tissue Neoplasms / Q000175:diagnosis / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D015514:Oncogene Proteins, Fusion; C116512:SYT-SSX fusion protein