Synthesis of 4-pyridoxolactone from pyridoxine using a combination of transformed Escherichia coli cells.
We developed a simple and efficient synthesis for 4-pyridoxolactone starting with pyridoxine and using a whole-cell biotransformation by two transformed Escherichia coli cell types. One set of transformed cells expressed pyridoxine 4-oxidase, catalase, and chaperonin, while the second set expressed pyridoxal 4-dehydrogenase. With this combination of cells, pyridoxine was first oxidized to pyridoxal, which was then dehydrogenated to 4-pyridoxolactone by pyridoxine 4-oxidase and pyridoxal 4-dehydrogenase, respectively. In a reaction mixture containing the two transformed cell types, 10 mM of pyridoxine was completely converted into 4-pyridoxolactone at 30 degrees C in 24 h. When starting with 80 mM of pyridoxine, it was necessary to add 0.5 mM or more of NAD(+) to complete the reaction.
Tamura
Asuka
A
Department of Agriculture, Faculty of Agriculture, Kochi University, 200 Monobe-Otsu, Nankoku, Kochi, Japan.
Yoshikane
Yu
Y
Yokochi
Nana
N
Yagi
Toshiharu
T
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods*; D002374:Catalase / Q000096:biosynthesis / Q000378:metabolism; D020134:Catalytic Domain; D018833:Chaperonins / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D007783:Lactones / Q000138:chemical synthesis*; D009243:NAD / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D011730:Pyridoxal / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D011736:Pyridoxine / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
C539642:4-pyridoxolactone; D007783:Lactones; D016877:Oxidants; D009243:NAD; D011730:Pyridoxal; D000429:Alcohol Oxidoreductases; C487815:pyridoxine 4-oxidase; D002374:Catalase; D018833:Chaperonins; D011736:Pyridoxine