[Adenoviral-mediated APE/Ref-1 expression protects rat spiral ganglion cells from oxidative damage].
OBJECTIVE
To address the question if apurinic/apyrimidinic endonuclease/redox factor 1 (APE/Ref-1) involved in preventing spiral ganglion cells oxidative damage after oxidative stress.


METHODS
Primary cultured rat spiral ganglion cells were infected with the adenovirus containing APE/Ref-1 for 48 h, then treated with H2O2 (0, 10, 25, 50, 100, 300 micromol/L) for 1 h, and finally changed back into normal medium. Western blot were used to detect the level of APE/Ref-1 protein in the infected cells to ensure APE/Ref-1 over expression as a result of adenovirus infection. The cell viability was determined by MTT and the apoptosis of spiral ganglion cells was determined by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).


RESULTS
Western blot showed that infection of adenovirus resulted in APE/Ref-1 over expression in the spiral ganglion cells. Over expression of APE/Ref-1 significantly improved cell viability in cultures treated with different concentration H2O2 from 50 to 300 micromol/L However, the apoptosis of cells was significantly inhibited.


CONCLUSIONS
Over expression of APE/Ref-1 could protect spiral ganglion cells from oxidative damage.
Jiang
Zhen-dong
ZD
Department of Otorhinolarynogology Head and Neck Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhang
Xue-yuan
XY
Yuan
Wei
W
Wei
Yun-jun
YJ
Zhong
Cheng
C
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000473:pathology*
D006861:Hydrogen Peroxide; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase