A new quinoline sensitizer-centered lanthanide chelate and its use for protein labling on Ni-NTA beads for TR LRET assays.
A quinoline sensitizer-centered lanthanide chelate system of novel design for TR-LRET was prepared; it exhibited high labelling efficiency with a his-tagged protein (ERalpha-LBD) on the Ni-NTA beads, using a mixed metal chelate protocol, and it functioned well in TR-LRET protein-protein interaction assays.
Kim
Sung Hoon
SH
Department of Chemistry of University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA. jkatzene@uiuc.edu.
Ge
Pinghua
P
Katzenellenbogen
John A
JA
D002614:Chelating Agents / Q000737:chemistry*; D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer; D028581:Lanthanoid Series Elements / Q000138:chemical synthesis / Q000737:chemistry*; D008863:Microspheres; D008956:Models, Chemical; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D009942:Organometallic Compounds; D011506:Proteins; D011804:Quinolines; C088321:nickel nitrilotriacetic acid; D009571:Nitrilotriacetic Acid