title: A suicidal DNA vaccine co-expressing two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus antigens induce protective responses.
abstract: We constructed a suicidal DNA vaccine pSFV-ORF5m/ORF6 co-expressing GP5m (a modified GP5) and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV). In mice immunization, specific immune responses were elicited by the suicidal DNA vaccine pSFV-ORF5m/ORF6. The immunogenicity and protective efficiency was then evaluated in piglets immunized with pSFV-ORF5m/ORF6 before virus challenge: PRRSV-specific neutralizing antibodies and lymphocyte proliferative responses were developed. Post-PRRSV challenge, these immune responses were further boosted and partial protection was obtained.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shi-zi-shan Street, 430070 Wuhan, Hubei, People's Republic of China.
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Jiang
authors.forename: Liurong
authors.initials: L
authors.lastname: Fang
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Pan
authors.forename: Rui
authors.initials: R
authors.lastname: Luo
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D007668:Kidney / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D009500:Neutralization Tests; D014066:Palatine Tonsil / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000821:virology; D013552:Swine; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; C525999:M protein, porcine reproductive and respiratory syndrome virus; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; C114789:glycoprotein 5, PRRSV