Miniaturized hollow fiber assisted liquid-phase microextraction and gas chromatography-mass spectrometry for the measurement of progesterone in human serum.
Miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) and gas chromatography-mass spectrometry (GC-MS) for the determination of progesterone in human serum sample is described. The detection limit and the quantification limit of progesterone in human serum sample are 0.5 and 2 ng mL(-1) (ppb), respectively. The calibration curve for progesterone is linear with a correlation coefficient of >0.999 in the range of 2-500 ng mL(-1). The average recoveries of progesterone in human serum samples spiked with 5, 50 and 200 ng mL(-1) progesterone are 97.4% (R.S.D.: 9.3%), 100.3% (R.S.D.: 4.7%) and 99.8% (R.S.D.: 3.4%), respectively. This simple, accurate, sensitive and selective analytical method can be applicable for the determination of progesterone in human serum samples.
Kawaguchi
Migaku
M
National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8563, Japan. m-kawaguchi@aist.go.jp
Takatsu
Akiko
A
D005591:Chemical Fractionation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008904:Miniaturization; D011374:Progesterone / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D011374:Progesterone