Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola.
The biosynthetic gene cluster of brassicicene C was identified in Alternaria brassicicola strain ATCC 96836 from genome database search. In vivo and in vitro study clearly revealed the function of Orf8 and Orf6 as a fusicoccadiene synthase and methyltransferase, respectively. The understanding toward the biosynthetic pathway promises construction of this type of diterpene compounds with genetic engineering.
Minami
Atsushi
A
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan. aminami@mail.sci.hokudai.ac.jp
Tajima
Naoto
N
Higuchi
Yusuke
Y
Toyomasu
Tomonobu
T
Sassa
Takeshi
T
Kato
Nobuo
N
Dairi
Tohru
T
D000528:Alternaria / Q000235:genetics*; D002474:Cell-Free System; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004224:Diterpenes / Q000737:chemistry*; D015195:Drug Design; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D016678:Genome; D008780:Methyltransferases / Q000235:genetics; D008956:Models, Chemical; D008957:Models, Genetic; D005810:Multigene Family; D010936:Plant Extracts / Q000378:metabolism*
D003470:Culture Media; D004224:Diterpenes; D010936:Plant Extracts; C539991:brassicicene C; D008780:Methyltransferases