title: Dynamic rejection of colloidal particles with generating dextran by enzymatic reaction.
abstract: Dextransucrase forms a complex with dextran during an enzymatic reaction with sucrose. Using its enzymatic character, we performed a continuous and dynamic rejection of colloidal particles by generating dextran with dextransucrase immobilized in an inorganic porous membrane. Inorganic membranes having 1.9 and 3.0 U/g of immobilized dextransucrase, and 4.1 and 9.4 mg/g of generated dextran, respectively, had constant rejection percentages for 55 and 100 nm colloidal particles in permeating solutions. On the other hand, permeating sucrose solutions containing colloidal particles through a dextran-immobilized membrane dynamically increased the rejection percentages of the colloidal particles owing to dextran generation via enzymatic reaction. The dynamic increase was due to the gradually generating dextran dynamically occupying the membrane pore with its steric volume.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Saga University, Saga 840-8502, Japan. kawakita@cc.saga-u.ac.jp
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Kawakita
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Kohshi
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Seto
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ohto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Harada
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Inoue
mesh_terms: D003102:Colloids / Q000737:chemistry*; D003911:Dextrans / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D008567:Membranes, Artificial; D008863:Microspheres; D013395:Sucrose / Q000378:metabolism
chemical_list: D003102:Colloids; D003911:Dextrans; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D013395:Sucrose; D005964:Glucosyltransferases; C020025:dextransucrase