Hydrogen peroxide biosensor based on the direct electrochemistry of myoglobin immobilized on silver nanoparticles doped carbon nanotubes film.
A novel H(2)O(2) biosensor has been fabricated based on the direct electrochemistry and electrocatalysis of myoglobin (Mb) immobilized on silver nanoparticles doped carbon nanotubes film with hybrid sol-gel techniques. A pair of redox peaks with peak separation of 160 mV and formal potential of -0.295V was observed at this composite film, corresponding to the direct electrochemistry of Mb. The heterogeneous rate constant was estimated to be 0.41s(-1). Under optimum conditions, the amperometric determination of H(2)O(2) was performed with a linear range of 2.0 x 10(-6)-1.2 x 10(-3)molL(-1) and a detection limit of 3.6 x 10(-7)mol/L (S/N=3). The Michealis-Menten constant was also estimated to be 1.62mmolL(-1). The proposed biosensor showed favorable reproducibility, stability, selectivity and accuracy, and has been used to determine H(2)O(2) in real samples with favorable recoveries.
Liu
Chuan-Yin
CY
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Hu
Ji-Ming
JM
D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
D009211:Myoglobin; D037742:Nanotubes, Carbon; D012834:Silver; D006861:Hydrogen Peroxide