title: Spent culture medium from virulent Borrelia burgdorferi increases permeability of individually perfused microvessels of rat mesentery.
abstract: BACKGROUND
  Lyme disease is a common vector-borne disease caused by the spirochete Borrelia burgdorferi (Bb), which manifests as systemic and targeted tissue inflammation. Both in vitro and in vivo studies have shown that Bb-induced inflammation is primarily host-mediated, via cytokine or chemokine production that promotes leukocyte adhesion/migration. Whether Bb produces mediators that can directly alter the vascular permeability in vivo has not been investigated. The objective of the present study was to investigate if Bb produces a mediator(s) that can directly activate endothelial cells resulting in increases in permeability in intact microvessels in the absence of blood cells.
  
  
  RESULTS
  The effects of cell-free, spent culture medium from virulent (B31-A3) and avirulent (B31-A) B. burgdorferi on microvessel permeability and endothelial calcium concentration, [Ca(2+)](i), were examined in individually perfused rat mesenteric venules. Microvessel permeability was determined by measuring hydraulic conductivity (Lp). Endothelial [Ca(2+)](i), a necessary signal initiating hyperpermeability, was measured in Fura-2 loaded microvessels. B31-A3 spent medium caused a rapid and transient increase in Lp and endothelial [Ca(2+)](i). Within 2-5 min, the mean peak Lp increased to 5.6+/-0.9 times the control, and endothelial [Ca(2+)](i) increased from 113+/-11 nM to a mean peak value of 324+/-35 nM. In contrast, neither endothelial [Ca(2+)](i) nor Lp was altered by B31-A spent medium.
  
  
  CONCLUSIONS
  A mediator(s) produced by virulent Bb under culture conditions directly activates endothelial cells, resulting in increases in microvessel permeability. Most importantly, the production of this mediator is associated with Bb virulence and is likely produced by one or more of the 8 plasmid(s) missing from strain B31-A.
authors.affiliation: Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV, USA.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
authors.forename: Michael R
authors.initials: MR
authors.lastname: Miller
authors.forename: Md
authors.initials: M
authors.lastname: Motaleb
authors.forename: Nyles W
authors.initials: NW
authors.lastname: Charon
authors.forename: Pingnian
authors.initials: P
authors.lastname: He
mesh_terms: D000818:Animals; D025065:Borrelia burgdorferi / Q000472:pathogenicity*; D002118:Calcium / Q000378:metabolism; D002199:Capillary Permeability / Q000502:physiology*; D003470:Culture Media; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D008643:Mesentery / Q000098:blood supply*; D055806:Microvessels / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014774:Virulence
chemical_list: D003470:Culture Media; D002118:Calcium