Effect of high glucose concentration on VEGF and PEDF expression in cultured retinal Müller cells.
To explore the effect of high glucose concentration on the expression of vascular endothelial growth factor (VEGF) and pigment epithelium derived factor (PEDF) in the cultured rat retinal Müller cells. Rat Müller cells were cultured and RT-PCR and Western-blot analysis were used to measure the levels of VEGF and PEDF in cultured Müller cells at different high glucose concentrations. Under 10, 20, 30 mmol/L high glucose conditions, the levels of VEGF mRNA and protein increased and the levels of PEDF mRNA and protein decreased. These results suggest that the VEGF and PEDF expression in Müller cells are unbalance under high glucose concentration, which contribute to retinal neovascularization in diabetic retinopathy.
Mu
Hua
H
Department of Ophthalmology, Eye Hospital, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Road, 150001 Harbin, China. muhua998@sohu.com
Zhang
Xiao-Mei
XM
Liu
Jian-Ju
JJ
Dong
Li
L
Feng
Zhuo-Lei
ZL
D000818:Animals; D002478:Cells, Cultured; D005136:Eye Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015843:Serpins / Q000096:biosynthesis* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D005947:Glucose; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C467498:vascular endothelial growth factor A, rat