Bioconjugation of biotin to the interfaces of polymeric micelles via in situ click chemistry.
Azido-containing amphiphilic triblock copolymer poly(ethylene glycol)-b-poly(azidoethyl methacrylate)-b-poly(methyl methacrylate) (PEG-b-PAzEMA-b-PMMA) was prepared by postpolymerization functionalization of poly(ethylene glycol)-b-poly(hydroxyethyl methacrylate)-b-poly(methyl methacrylate) (PEG-b-PHEMA-b-PMMA). In aqueous media, PEG-b-PAzEMA-b-PMMA self-assembled into spherical micelles with the azide groups at the hydrophobic/hydrophilic interface due to the molecular architecture. Biotin was conjugated to the micelles by in situ click chemistry between azide groups and alkynated biotin, resulting in the formation of a functional interface between the hydrophilic shell and the hydrophobic core. The bioavailability of biotin to avidin was demonstrated by an avidin/4'-hydroxyazobenzene-2-carboxylic acid (avidin/HABA) assay, transmission electron microscopy, and dynamic light scattering investigations.
Wang
Xiaojuan
X
Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Tianjin 300071, People's Republic of China.
Liu
Li
L
Luo
Yan
Y
Zhao
Hanying
H
D001710:Biotin / Q000138:chemical synthesis / Q000737:chemistry*; D008823:Micelles; D015394:Molecular Structure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
D008823:Micelles; D011108:Polymers; C535254:poly(ethylene glycol)-b-poly(azidoethyl methacrylate)-b-poly(methyl methacrylate); D011092:Polyethylene Glycols; D001710:Biotin; D019904:Polymethyl Methacrylate