Straightforward protein immobilization using redox-initiated poly(methyl methacrylate) polymerization.
Rigid poly(methyl methacrylate) (PMMA) biochips directly modified with active protein spots were obtained, using a redox-initiated PMMA polymerization process. The protein immobilization mechanism is believed to be a combination of both a covalent binding through transient amino acid radical generation and a direct entrapment of the biomolecules in the PMMA polymer. Three different immunoassays (binding, capture, and sandwich) were performed using the developed system, and really promising limits of detection (160-200pg/mL) were obtained, demonstrating a novel straightforward route to fabricate plastic biochips.
Heyries
Kevin A
KA
Laboratoire de Genie Enzymatique et Biomoleculaire, Universite Lyon 1 - CNRS 5246 ICBMS, Bat CPE, 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France.
Blum
Loïc J
LJ
Marquette
Christophe A
CA
D055767:Immobilized Proteins / Q000737:chemistry*; D007118:Immunoassay; D008163:Luminescent Measurements; D015394:Molecular Structure; D010084:Oxidation-Reduction; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*
D055767:Immobilized Proteins; D019904:Polymethyl Methacrylate