A method for examining glycans surface expression of yeasts by flow cytometry.
Recognition of pathogenic yeasts by host cells is based on components of the yeast cell wall, which are considered part of its virulence attributes. Cell wall glycans play an important role in the continuous interchange that regulates the balance between saprophytism and parasitism and between resistance and infection. Flow cytometry is a useful method for probing surface yeast glycans in order to compare their expression depending on strains and growth conditions. By using different monoclonal or polyclonal antibodies, levels of beta- and alpha-linked mannosides as well as beta-glucans can be successfully evaluated by flow cytometry methods. The cytometric method we describe here represents a useful tool to investigate to what extent yeasts are able to regulate their glycan surface expression and therefore modify their virulence properties.
Martínez-Esparza
Maria
M
Departamento de Bioquímica y Biología Molecular (B) e Inmunología, Facultad de Medicina, Universidad de Murcia, Murcia, Spain.
Sarazin
Aurore
A
Poulain
Daniel
D
Jouault
Thierry
T
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods*; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000276:immunology; D014774:Virulence / Q000276:immunology; D015003:Yeasts / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D011134:Polysaccharides