title: Insight into long-term histological, proliferative and apoptotic modifications in ileal orthotopic neobladder and conduit mucosa.
abstract: OBJECTIVE
  To assess the long-term histological, apoptotic and proliferating alterations of the intestinal mucosa of ileal conduits and orthotopic neobladders.
  
  
  METHODS
  Fifty patients (46 males, 4 females), aged 52-78 years, who underwent urinary diversion with either ileal orthotopic neobladder (group ON, 20 patients) or conduit (group IC, 30 patients) from 2001 to 2005, were included in this prospective study. Ileal samples were collected during surgery (controls) and by random mucosal biopsies 6, 12, 24, 36 and 48 months later. Histological (villi height, crypt depth, eosinophil cell count), proliferation (Ki67 immunochemistry), and apoptotic (Bcl-2 immunochemistry, TUNEL) parameters were assessed.
  
  
  RESULTS
  During the 4-year follow-up, we recorded progressive villi area, height and crypt depth reduction, mucosa flattening, and inflammatory and eosinophilic infiltration. Villi height: crypt depth ratio showed a statistically significant difference (P < 0.05) between the two groups from the 6th month. Dysplasia, metaplasia, and neoplasia were not observed. Bcl-2 values showed a progressive increase until 24 months in group ON and 12 months in group IC, followed by a decline thereafter. Ki-67 values showed a progressive increase after 6 months in group ON and an increase until 24 months followed by a decline thereafter in group IC. TUNEL showed two peaks, at 24 and 48 months.
  
  
  CONCLUSIONS
  Histological adaptation was revealed in both groups, with statistically significant differences in favor of orthotopic substitution. Proliferative and apoptotic pathways are implicated as demonstrated by relevant modifications of Bcl-2, Ki-67 and TUNEL, in accord with the histological adaptation.
authors.affiliation: 2nd Department of Urology, School of Medicine, University of Athens, Sismanoglio General Hospital, Athens, Greece. athan_dell@yahoo.gr
authors.forename: Athanasios E
authors.initials: AE
authors.lastname: Dellis
authors.forename: Maria
authors.initials: M
authors.lastname: Demonakou
authors.forename: Athanasios G
authors.initials: AG
authors.lastname: Papatsoris
authors.forename: Michail
authors.initials: M
authors.lastname: Chrisofos
authors.forename: Aris
authors.initials: A
authors.lastname: Bamias
authors.forename: Charalambos
authors.initials: C
authors.lastname: Deliveliotis
mesh_terms: D000222:Adaptation, Physiological; D000368:Aged; D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis*; D049109:Cell Proliferation; D015653:Cystectomy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007413:Intestinal Mucosa / Q000033:anatomy & histology* / Q000737:chemistry*; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D013997:Time Factors; D014547:Urinary Diversion
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2