Activation of muscarinic M-3 receptor may decrease glucose uptake and lipolysis in adipose tissue of rats.
Role of muscarinic receptor in the regulation of glucose uptake or lipolysis in adipose tissue remained unclear. In epididymal white adipose tissue (WAT) isolated from Wistar rats, we observed that acetylcholine (ACh) attenuated the insulin-stimulated glucose uptake and the release of glycerol from WAT in a concentration-dependent manner. Using the blockade of specific antagonists, both actions of ACh were characterized mainly due to an activation of M3 receptors. In the presence of various inhibitors for PLC-PKC pathway, ACh-decreased glucose uptake was also reversed. Taken together, these results suggest that muscarinic M3 receptor is involved in the regulation of glucose uptake and/or lipolysis in adipose tissue.
Yang
Ting-Ting
TT
Graduate Institute of Basic Medical Science, China Medical University, Taichung City, Taiwan, ROC.
Chang
Cheng-Kuei
CK
Tsao
Chiung-Wen
CW
Hsu
Ya-Mei
YM
Hsu
Chao-Tien
CT
Cheng
Juei-Tang
JT
D000109:Acetylcholine / Q000378:metabolism* / Q000494:pharmacology; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007329:Insulin Antagonists / Q000378:metabolism / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D043587:Receptor, Muscarinic M3 / Q000187:drug effects / Q000378:metabolism*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism
D004791:Enzyme Inhibitors; D007328:Insulin; D007329:Insulin Antagonists; D043587:Receptor, Muscarinic M3; D010738:Type C Phospholipases; D005947:Glucose; D000109:Acetylcholine; D005990:Glycerol