Interaction between Arabidopsis Ca2+/H+ exchangers CAX1 and CAX3.
In plants, high capacity tonoplast cation/H(+) antiport is mediated in part by a family of CAX (cation exchanger) transporters. Functional association between CAX1 and CAX3 has previously been inferred; however, the nature of this interaction has not been established. Here we analyze the formation of "hetero-CAX" complexes and their transport properties. Co-expressing both CAX1 and CAX3 mediated lithium and salt tolerance in yeast, and these phenotypes could not be recapitulated by expression of deregulated versions of either transporter. Coincident expression of Arabidopsis CAX1 and CAX3 occurs during particular stress responses, flowering, and seedling growth. Analysis of cax1, cax3, and cax1/3 seedlings demonstrated similar stress sensitivities. When plants expressed high levels of both CAXs, alterations in transport properties were evident that could not be recapitulated by high level expression of either transporter individually. In planta coimmunoprecipitation suggested that a protein-protein interaction occurred between CAX1 and CAX3. In vivo interaction between the CAX proteins was shown using a split ubiquitin yeast two-hybrid system and gel shift assays. These findings demonstrate cation exchange plasticity through hetero-CAX interactions.
Zhao
Jian
J
United States Department of Agriculture/Agricultural Research Service, Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030-23600, USA.
Shigaki
Toshiro
T
Mei
Hui
H
Guo
Ying-Qing
YQ
Cheng
Ning-Hui
NH
Hirschi
Kendal D
KD
D017920:Antiporters / Q000096:biosynthesis* / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D027682:Cation Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D035264:Flowers / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017136:Ion Transport / Q000502:physiology; D036226:Seedlings / Q000201:enzymology; D013312:Stress, Physiological / Q000502:physiology
D017920:Antiporters; D029681:Arabidopsis Proteins; C506406:CAX3 protein, Arabidopsis; D027682:Cation Transport Proteins; C027614:calcium-hydrogen antiporters