title: Localization of Entamoeba histolytica amebopore in amebic liver abscesses in hamsters.
abstract: Amebopore was purified from axenically grown trophozoites of the Entamoeba histolytica strain HM1:IMSS. The purification procedure involved Mono Q anion-exchange chromatography and electroelution. Sequence analysis of the final product revealed that amebopore A was completely pure. Polyclonal antibodies against the purified amebopore were obtained from rabbits, and Western blot studies demonstrated their specificity. Sections of experimental, acute (1, 2, 3, and 4 days), amebic liver abscesses produced in hamsters were stained with the anti-amebopore antibody; in all the analyzed stages, amebopore appeared as a constitutively expressed cytoplasmic molecule in trophozoites. No extracellular or hepatocyte-membrane amebopore was found. This study is the first to trace amebopore in an in vivo model of amebic liver abscesses.
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, National Autonomous University of Mexico and General Hospital, Mexico City, Mexico. metaugus@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: González
authors.forename: D
authors.initials: D
authors.lastname: Monterrubio
authors.forename: M
authors.initials: M
authors.lastname: Nequiz
authors.forename: R
authors.initials: R
authors.lastname: López
authors.forename: A
authors.initials: A
authors.lastname: Olivos
authors.forename: C
authors.initials: C
authors.lastname: García de Léon
authors.forename: E
authors.initials: E
authors.lastname: Tello
authors.forename: N
authors.initials: N
authors.lastname: Salaiza
authors.forename: E
authors.initials: E
authors.lastname: Ramos
authors.forename: M
authors.initials: M
authors.lastname: Gudiño
authors.forename: I
authors.initials: I
authors.lastname: Montfort
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Tamayo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D007150:Immunohistochemistry; D008101:Liver Abscess, Amebic / Q000469:parasitology*