[Molecular techniques in mycology].
An increasing number of molecular techniques for the diagnosis of fungal infections have been developed in the last few years, due to the growing prevalence of mycoses and the length of time required for diagnosis when classical microbiological methods are used. These methods are designed to resolve the following aspects of mycological diagnosis: a) Identification of fungi to species level by means of sequencing relevant taxonomic targets; b) early clinical diagnosis of invasive fungal infections; c) detection of molecular mechanisms of resistance to antifungal agents; and d) molecular typing of fungi. Currently, these methods are restricted to highly developed laboratories. However, some of these techniques will probably be available in daily clinical practice in the near future.
Rodríguez-Tudela
Juan Luis
JL
Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, España. jlrtudela@isciii.es
Cuesta
Isabel
I
Gómez-López
Alicia
A
Alastruey-Izquierdo
Ana
A
Bernal-Martínez
Leticia
L
Cuenca-Estrella
Manuel
M
D000935:Antifungal Agents / Q000627:therapeutic use; D000946:Antigens, Fungal / Q000097:blood; D004271:DNA, Fungal / Q000032:analysis; D021901:DNA, Intergenic / Q000032:analysis; D025141:Drug Resistance, Fungal; D042241:Early Diagnosis; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007753:Laboratories / Q000600:supply & distribution; D025202:Molecular Diagnostic Techniques / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology; D013045:Species Specificity
D000935:Antifungal Agents; D000946:Antigens, Fungal; D004271:DNA, Fungal; D021901:DNA, Intergenic