Drosophila mutants in phospholipid signaling have reduced olfactory responses as adults and larvae.
In this paper, we show that mutants in the gene stambhA (stmA), which encodes a putative phosphatidylinositol 4,5 bisphosphate-diacylglycerol lipase, exhibit a significant reduction in the amplitudes of odor-evoked responses recorded from the antennal surface of adult Drosophila. This lends support to previously published findings that olfactory transduction in Drosophila requires a phospholipid intermediate. Mutations in stmA also affect the olfactory behavior response of larvae. Moreover, there is a requirement for G(q)alpha and phospholipase Cbeta function in larval olfaction. The results suggest that larval olfactory transduction, like that of the adult, utilizes a phospholipid second messenger, generated by the activation of G(q)alpha and Plcbeta21c, and modulated by the stmA gene product.
Kain
Pinky
P
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
Chandrashekaran
Shanti
S
Rodrigues
Veronica
V
Hasan
Gaiti
G
D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004594:Electrophysiology; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D007814:Larva / Q000235:genetics / Q000502:physiology; D008071:Lipoprotein Lipase / Q000145:classification / Q000235:genetics*; D024521:Mushroom Bodies / Q000166:cytology / Q000254:growth & development; D009154:Mutation / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D009812:Odorants; D009833:Olfactory Pathways / Q000254:growth & development* / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics*; D012903:Smell / Q000235:genetics / Q000502:physiology*
D029721:Drosophila Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008071:Lipoprotein Lipase; D043802:GTP-Binding Protein alpha Subunits, Gq-G11