Comparative proteomic analysis of apomictic monosomic addition line of Beta corolliflora and Beta vulgaris L. in sugar beet.
Apomixis refers to a process in which plants produce seed without fertilization through female syngamy that produces embryos genetically identical to the maternal parent. In sugar beet, interspecific hybrids between diploid Beta vulgaris and tetraploid Beta corolliflora were established and monosomic addition line M14 was selected because of the apomictic phenotype. By using two-dimensional electrophoresis gels we identified the proteins which were differently expressed between the M14 and B. vulgaris. A total of 27 protein spots which varied expressed between lines were isolated and successfully identified with MALDI-TOF MS. Among them five protein spots were found to be only presented in M14 and two protein spots only expressed in Beta. According to their functional annotations described in Swissprot database, these proteins were, respectively, involved in important biological pathways, such as cell division, functionally classified using the KEGG functional classification system. The result may be useful for us to better understand the genetic mechanism of apomixes.
Zhu
Hong
H
Department of Biology, College of Life and Environment Science, Harbin Normal University, 150080 Harbin, China. zhuhong10000@163.com
Bi
Ying-Dong
YD
Yu
Li-Jie
LJ
Guo
De-Dong
DD
Wang
Bai-Chen
BC
D027461:Beta vulgaris / Q000235:genetics / Q000378:metabolism*; D019663:Chenopodiaceae / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D011003:Ploidies; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012098:Reproduction / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010940:Plant Proteins; D020543:Proteome