A room temperature ionic liquid as convenient solvent for the oxidative folding of conopeptides.
We report the first example of conopeptide oxidation performed in a biocompatible ionic liquid, 1-ethyl-3-methylimidazolium acetate ([C(2)mim][OAc]), which enables the efficient formation of both hydrophilic and poorly water-soluble conotoxins compared with conventional methods. Moreover, the method features a high-concentration approach ultimately leading to higher yields at reduced separation effort.
Miloslavina
Alesia A
AA
Center for Molecular Biomedicine, Department of Biochemistry, Friedrich Schiller University Jena, Jena, Germany.
Leipold
Enrico
E
Kijas
Michael
M
Stark
Annegret
A
Heinemann
Stefan H
SH
Imhof
Diana
D
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D020916:Conotoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D051381:Rats; D015222:Sodium Channels / Q000378:metabolism; D012997:Solvents / Q000737:chemistry*; D013696:Temperature
D020916:Conotoxins; D007477:Ions; D009124:Muscle Proteins; D010455:Peptides; C466180:Scn4a protein, rat; D015222:Sodium Channels; D012997:Solvents