title: Relationship of polymorphisms located in tumor necrosis factor region and HLA loci among Croatians.
abstract: Polymorphism of TNFa, TNFb, and TNFd microsatellites, linkage disequilibrium (LD) within TNF region as well as relationship of TNF microsatellites with alleles at HLA-A, -B, and -DRB1 loci was investigated on a sample of 160 Croatians, previously typed for HLA-A, -B, and -DRB1 loci. Analysis of the relationship of TNF alleles and HLA specificities revealed that very strong associations exist between TNFa2, TNFb3, and TNFd2 alleles and HLA-A*01, -B*08, and -DRB1*03 specificities, therefore placing them in the 8.1 ancestral haplotype. Similar findings were observed for TNFa11, TNFb4, and TNFd4 alleles and HLA specificities, which are a part of the 7.1 ancestral haplotype. Finally, multiple associations with significant P-values were also observed between TNFa10, TNFb4, and TNFd4 microsatellite alleles and HLA-A*02, -B*18, -DRB1*11 specificities which form the 18.3 ancestral hapolotype.
authors.affiliation: Department for Laboratory Diagnostics, Tissue Typing Centre, University Hospital Centre Zagreb, Zagreb, Croatia. katarinastingl@gmail.com
authors.forename: Katarina
authors.initials: K
authors.lastname: Stingl
authors.forename: Zorana
authors.initials: Z
authors.lastname: Grubic
authors.forename: Helena
authors.initials: H
authors.lastname: Car
authors.forename: Renata
authors.initials: R
authors.lastname: Zunec
mesh_terms: D000483:Alleles; D002460:Cell Line; D016009:Chi-Square Distribution; D017523:Croatia; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D006680:HLA Antigens; D014409:Tumor Necrosis Factor-alpha