title: On-line coupled dynamic sonication-assisted extraction-liquid chromatography for the determination of phenolic acids in Lamiaceae herbs.
abstract: A fast and efficient on-line coupled dynamic sonication-assisted extraction-liquid chromatography (DSAE-LC) method was developed for the determination of phenolic acids in basil, oregano, rosemary, sage, spearmint and thyme. The extraction and chromatography were coupled via a solid-phase trap filled with strong anion exchange material. The relative standard deviations (RSDs) for the retention times were less than 0.4% and those for the peak heights less than 3% except for gallic acid (RSD 1.2% for the retention times and 11% for the peak heights). Limits of detection were below approximately 3ng.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FIN-00014, Helsinki, Finland.
authors.forename: Maarit
authors.initials: M
authors.lastname: Kivilompolo
authors.forename: Tuulia
authors.initials: T
authors.lastname: Hyötyläinen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis*; D004867:Equipment Design; D062385:Hydroxybenzoates / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013010:Sonication / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002934:Cinnamates; D062385:Hydroxybenzoates