Identification and characterization of a new coleviroid (CbVd-5).
A viroid-like RNA was detected from coleus (Coleus blumei) in China. It consisted of 274 nucleotides and had 66% sequence identity with a member of the closest known viroid species. The predicted secondary structure is rod-shaped with extensive base pairing, and it has the conserved region characteristic of the genus Coleviroid. Two terminal sequences that are highly conserved among some members of the genus were also identified. The viroid-like RNA was successfully transmitted to coleus by slash-inoculation. This viroid was identified as a new member of the genus Coleviroid, and we tentatively propose the name Coleus blumei viroid 5 (CbVd-5).
Hou
Wan-Ying
WY
State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West No. 2, Beijing 100193, People's Republic of China.
Sano
Teruo
T
Li
Feng
F
Wu
Zu-Jian
ZJ
Li
Li
L
Li
Shi-Fang
SF
D001483:Base Sequence; D002681:China; D031330:Coleus / Q000821:virology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014772:Viroids / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence
D012367:RNA, Viral