[The correlation between the polyomavirus DNA load and the dose of immunosuppressant].
OBJECTIVE
To explore the correlation between the polyomavirus DNA load and the dose of immunosuppressant in patients with allogene bone marrow transplantation (allo-BMT) for preventing the development of post-transplantational hemorrhagic cystitis.


METHODS
Serial blood and urine samples from 122 cases of allo-BMT recipients were obtained and DNA was extracted from urine samples. Polyomavirus DNA-specific probe was synthesized and Fluorescence quantitative polymerase chain reaction was used for detecting the polyomavirus DNA loads and Fluorescence polarization immunoassay (FPIA) was performed for determining the dose of immunosuppressant Cyclosporin A (CsA) in blood.


RESULTS
The altered polyomavirus DNA load in urine was followed by concentration of CsA in blood. When the concentration of CsA in blood was higher than 86-105 ng/ml, the positive rate of polyomavirus DNA load was significantly increased and both presented the linable correlation.


CONCLUSIONS
In immunosuppression condition, polyomavirus DNA load correlated to the dose of immunosuppressant, which increased the risk of post-transplantational hemorrhagic cystitis.
Cai
Peng
P
Beijing Dao Pei Hospital, Beijing 100039, China.
Teng
Zhi-Ping
ZP
Wu
Tong
T
Zhang
Jian-Feng
JF
Liu
Ying
Y
Wang
Zheng
Z
Zheng
Wan-Xia
WX
Xu
Guo-Lin
GL
Tong
Chun-Rong
CR
D016026:Bone Marrow Transplantation / Q000009:adverse effects; D016572:Cyclosporine / Q000009:adverse effects* / Q000097:blood; D003556:Cystitis / Q000517:prevention & control / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000652:urine; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects* / Q000097:blood; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000517:prevention & control / Q000821:virology*; D014184:Transplantation, Homologous / Q000009:adverse effects; D019562:Viral Load
D004279:DNA, Viral; D007166:Immunosuppressive Agents; D016572:Cyclosporine