Innate immunity prevents tissue invasion by Entamoeba histolytica.
Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Guérin, lipopolysaccharide, complete Freund's adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7 days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68 kDa with peroxidase activity.
Shibayama
Mineko
M
Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.2508 Zacatenco, CP07360, Mexico, DF.
Rivera-Aguilar
Víctor
V
Barbosa-Cabrera
Elizabeth
E
Rojas-Hernández
Saúl
S
Jarillo-Luna
Adriana
A
Tsutsumi
Víctor
V
Pacheco-Yepez
Judith
J
Campos-Rodríguez
Rafael
R
D000818:Animals; D000917:Antibody Formation; D006224:Cricetinae; D004404:Dysentery, Amebic / Q000276:immunology / Q000469:parasitology; D004748:Entamoeba histolytica / Q000276:immunology* / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000276:immunology* / Q000469:parasitology* / Q000517:prevention & control*; D005122:Exudates and Transudates / Q000276:immunology / Q000469:parasitology; D005620:Freund's Adjuvant / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D007274:Injections, Intraperitoneal; D008101:Liver Abscess, Amebic / Q000276:immunology / Q000469:parasitology; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000469:parasitology; D008297:Male; D008899:Mineral Oil; D009163:Mycobacterium bovis / Q000276:immunology; D009569:Nitric Oxide / Q000276:immunology; D009195:Peroxidase / Q000276:immunology; D053058:Trophozoites / Q000276:immunology
D009569:Nitric Oxide; D008899:Mineral Oil; D005620:Freund's Adjuvant; D009195:Peroxidase