Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides.
We report our contribution to the systematic investigation of peptide fragmentations performed on high-performance Tof equipment, operating in MS and MS/MS modes, such as ESI-QqTof and MALDI-Tof/Tof instruments that are commonly available today in proteomic laboratories. Whereas the former analyzer's configuration provides low-energy collision-induced dissociations (CID), the latter allows tunable activation methods of the selected parent ion to induce either metastable laser-induced dissociations (LID) or high-energy CID ('gas on spectra LID'). Fragmentation of the monoprotonated ion of 53 peptides (FW 807-2853 g/mol) was undertaken upon low-energy CID on an ESI-QTof mass spectrometer (Waters) as well as high-energy CID and LID conditions on a MALDI Ultraflex mass spectrometer (Bruker). Systematic comparison of MS/MS spectra provided useful information on the performance of each piece of equipment for efficient peptide sequencing and also insights into the observed fragmentation behaviors.
Shenar
Nawar
N
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-Universités Montpellier 1 et 2, Bâtiment Chimie (17), Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
Sommerer
Nicolas
N
Martinez
Jean
J
Enjalbal
Christine
C
D000577:Amides / Q000737:chemistry; D000641:Ammonia / Q000737:chemistry; D001120:Arginine / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D007834:Lasers; D008239:Lysine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014867:Water / Q000737:chemistry
D000577:Amides; D002264:Carboxylic Acids; D007477:Ions; D010455:Peptides; D014867:Water; D000641:Ammonia; D001120:Arginine; D008239:Lysine