title: Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients.
abstract: Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.
authors.affiliation: The Heart Research Institute, Camperdown, Sydney, NSW, Australia. morganp@hri.org.au
authors.forename: Philip E
authors.initials: PE
authors.lastname: Morgan
authors.forename: Allan D
authors.initials: AD
authors.lastname: Sturgess
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Davies
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D015331:Cohort Studies; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D011485:Protein Binding; D012720:Severity of Illness Index
chemical_list: D001798:Blood Proteins