The role of the antioxidants lycopene and vitamin E in the prevention of halothane-induced hepatotoxicity.
The aim of this study was to examine the effects of lycopene and vitamin E on halothane-induced hepatotoxicity. Thirty-five male albino Wistar rats were studied. The control group (group C) did not receive any treatment. Rats in group H were exposed to 1.5% halothane (in 50% oxygen/50% air) for 2 h on days 10 and 13. Group L received 25 mg/kg/day lycopene, group E received 100 IU/kg/day vitamin E and group LE received lycopene and vitamin E for 13 days. Similar to group H, groups L, E and LE were exposed to halothane. Total antioxidant capacity (TAC), total oxidant level (TOL) and sulfhydryl=thiol groups (SH) were measured. Histopathological examinations were carried out using light microscopy, and histopathological findings were graded on a scale of 0-6. There were no significant differences among the groups in TAC, TOL and SH values (P > 0.05). Liver injury was observed in the four treatment groups; the mean degree of damage was more severe in group H compared to groups E, L and LE: 2.14 +/- 0.37, 1.50 +/- 0.54, 0.85 +/- 0.69 and 0.83 +/- 0.75, respectively. This study found that both lycopene and vitamin E reduce halothane-induced hepatotoxicity, although the effect of vitamin E was not statistically significant.
Beştaş
Azize
A
Department of Anesthesiology and Reanimation, Firat University School of Medicine, Elazig, Turkey. abestas@firat.edu.tr
Kahramanoglu
Mithat
M
Erhan
Omer Lütfi
OL
Bolat
Esef
E
Ozercan
Ibrahim
I
Gürsu
Ferit
F
Gülcü
Funda
F
D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002338:Carotenoids / Q000494:pharmacology* / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004359:Drug Therapy, Combination; D006221:Halothane / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000077276:Lycopene; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use
D018685:Anesthetics, Inhalation; D000975:Antioxidants; D014810:Vitamin E; D002338:Carotenoids; D000077276:Lycopene; D006221:Halothane