Biorecognition of Escherichia coli K88 adhesin for glycated porcine albumin.
Escherichia coli (E. coli) that expresses galactose-reactive lectins, like K88 adhesin, causes high mortality among piglets. Carbohydrates that compete for adhesion could serve as an alternative for disease prevention. Porcine serum albumin (PSA) was modified by non-enzymatic glycation with lactose to produce PSA-Lac or PSA-Glc beta (1-4) Gal, as confirmed by reduction of available free amino groups, increased molecular mass and by Ricinus communis lectin recognition. E. coli K88 binds to PSA-Lac treatments containing three and four lactoses, respectively. In addition, PSA-Lac partially inhibited K88 strain adherence to mucins. These results suggest that neoglycoconjugates obtained by non-enzymatic glycation of proteins may serve in the prophylaxis of piglets' diarrhea.
Sarabia-Sainz
Andre-i
AI
Laboratorio de Bioquímica de Proteínas, Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., P.O. Box 1735, Hermosillo, Sonora, Mexico.
Ramos-Clamont
Gabriela
G
Candia-Plata
Ma María del Carmen
MM
Vázquez-Moreno
Luz
L
D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D000588:Amines; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000187:drug effects; D004586:Electrophoresis; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D007785:Lactose / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa; D014364:Tryptophan / Q000378:metabolism
D018830:Adhesins, Escherichia coli; D000588:Amines; D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D012709:Serum Albumin; D036781:Fimbriae Proteins; D014364:Tryptophan; D007785:Lactose