[Methylation of Id4 gene promoter in 4 malignant hematopoietic cell lines].
This study was purposed to investigate the status of Id4 gene promoter methylation in malignant hematologic cell lines. MS-PCR methods were used to detect the status of Id4 gene methylation in K562, HL-60, Ramous and CA46. Meanwhile bone marrow cells in healthy individuals and Hek937, a benign renal cell line, were involved as control. The results showed that Id4 gene kept unmethylated in bone marrow cells from healthy individuals and Hek937 cells, while Id4 gene was methylated in all of K562, HL-60, Ramous and CA46 cell lines. It is concluded that different from healthy bone marrow cells and non-malignant cell line Hek937, Id4 gene was methylated in leukemia and lymphoma cell lines including K562, HL-60, Ramous and CA46. The change of Id4 gene methylation is thought to be associated with occurrence of hematologic malignancies.
Zhao
Yu
Y
Department of Hematology, The General Hospital of PLA, Beijing 100853, China.
Wang
Quan-Shun
QS
Li
Hong-Hua
HH
Jing
Yu
Y
Bo
Jian
J
Wang
Shu-Hong
SH
Yu
Li
L
D045744:Cell Line, Tumor; D019175:DNA Methylation; D019337:Hematologic Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics*; D020014:K562 Cells; D011401:Promoter Regions, Genetic
C490128:ID4 protein, human; D051796:Inhibitor of Differentiation Proteins