Phenolic hydroxy groups incorporated for the peroxidase-catalyzed gelation of a carboxymethylcellulose support: cellular adhesion and proliferation.
The effect of Ph-OH group content on gelation time, mechanical properties, hydrophobicity, and cellular adhesiveness of hydrogels produced from carboxymethylcellulose derivatives is investigated. A higher Ph-OH group content induces faster gelation and yields more brittle and hydrophobic gels. After 4 h of seeding, a larger number of L929 fibroblasts adhere to the hydrogel of the CMC-Ph that contains 15.4 Ph-OH groups per 100 repeat units of uronic acid (97% adhesion rate) than to the gel of CMC-Ph with only 8.4 Ph-OH groups (62% adhesion rate). The results demonstrate that controlling the Ph-OH group content is an effective and useful way to control cellular adhesion and proliferation on the hydrogels, as well as gelation time and mechanical properties of the gels.
Ogushi
Yuko
Y
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. yogushi@chem-eng.kyushu-u.ac.jp
Sakai
Shinji
S
Kawakami
Koei
K
D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D007700:Kinetics; D051379:Mice; D010544:Peroxidases; D010636:Phenols / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
D020100:Hydrogels; D010636:Phenols; D010544:Peroxidases; D002266:Carboxymethylcellulose Sodium