title: Proton gradients produced by glucose oxidase microcapsules containing motor F0F1-ATPase for continuous ATP biosynthesis.
abstract: Glucose oxidase (GOD) microcapsules held together by cross-linker, glutaraldehyde (GA), are fabricated by the layer-by-layer (LbL) assembly technique. The lipid bilayer containing CF(0)F(1)-ATPase was coated on the outer shell of GOD microcapsules. Driven under the proton gradients produced by catalysis of GOD microcapsules for glucose, ATP is synthesized from ADP and inorganic phosphate catalyzed by the ATPase rotary catalysis. The results show here that ATPase reconstituted on the GOD microcapsules retains its catalytic activity.
authors.affiliation: Beijing National Laboratory for Molecular Science, International Joint Laboratory, CAS Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.forename: Xuehai
authors.initials: X
authors.lastname: Yan
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Yue
authors.initials: Y
authors.lastname: Cui
authors.forename: Kewei
authors.initials: K
authors.lastname: Wang
authors.forename: Dongxiang
authors.initials: D
authors.lastname: Li
authors.forename: Junbai
authors.initials: J
authors.lastname: Li
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001234:Aspergillus niger / Q000201:enzymology; D002214:Capsules / Q000737:chemistry; D004795:Enzyme Stability; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011522:Protons
chemical_list: D002214:Capsules; D011522:Protons; D000255:Adenosine Triphosphate; D005949:Glucose Oxidase; D006180:Proton-Translocating ATPases