Glycoproteomic analysis of plasma from patients with atopic dermatitis: CD5L and ApoE as potential biomarkers.
Atopic dermatitis (AD) is an inflammatory skin disorder that is both uncomfortable and distressing to patients, and its prevalence has been steadily increasing. It is obvious that the identification of efficient markers of AD in plasma would offer the possibility of effective diagnosis, prevention, and treatment strategies. In this study, a proteomic approach was used to analyze plasma glycoproteins from both children with AD and healthy child donors. Several protein spots showing significant quantitative changes in the AD patients were identified. Through sequential studies, it was confirmed that CD5L and ApoE were significantly up-regulated or down-regulated, respectively, in the plasma from AD patients compared with that from healthy donors. In addition, we suggest that the up-regulated CD5L in AD patients causes eosinophilia by inhibiting apoptosis or promoting the proliferation of eosinophils either in combination with or without IL-5. The glycoproteomic data in this study provides clues to understanding the mechanism of atopic alterations in plasma and suggests AD-related proteins can be used as candidate markers for AD.
Kim
Won Kon
WK
Translational Research Center, KRIBB, Daejeon 305-806, Korea.
Hwang
Hyang Ran
HR
Kim
Do Hyung
DH
Lee
Phil Young
PY
In
Yu Jung
YJ
Ryu
Hye Young
HY
Park
Sung Goo
SG
Bae
Kwang Hee
KH
Lee
Sang Chul
SC
D001057:Apolipoproteins E / Q000097:blood*; D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D049109:Cell Proliferation; D002648:Child; D003876:Dermatitis, Atopic / Q000378:metabolism*; D004802:Eosinophilia / Q000378:metabolism; D004804:Eosinophils / Q000502:physiology; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D015848:Interleukin-5 / Q000378:metabolism; D008297:Male; D040901:Proteomics; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B / Q000097:blood*
D001057:Apolipoproteins E; D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; C492821:CD5L protein, human; D006023:Glycoproteins; D015848:Interleukin-5; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B