DNA Checkerboard method for bacterial detection of microbiota from teeth and tongue biofilms. A preliminary study.
OBJECTIVE
The aim of this study was to determine the prevalence of 13 bacterial species in the dorsum tongue biofilm and compare its microbiota to that of subgingival samples in healthy adult subjects using DNA Checkerboard hybridization.


METHODS
A total of 40 samples, from subgingival and tongue biofilm, were enrolled into the study. All the samples were harvested and analyzed by DNA Checkerboard method for their content of 13 species of bacteria. Bacterial scores from each subject and location were averaged and the results of both sites were compared using the Wilcoxon signed ranks test.


RESULTS
All the microorganisms evaluated were detected in both tongue and subgingival samples, without significant differences in their prevalence.


CONCLUSIONS
The dorsum of the tongue was colonized by various microorganisms with similar prevalence as found in the subgingival biofilm of healthy individuals, suggesting that the tongue could be a potential microbial reservoir and may play a role in the recolonization of teeth surfaces.
Do Nascimento
C
C
Department of Dental Materials and Prosthodontics, University of São Paulo, São Paulo, Brazil.
Sato
S
S
Mardegan Issa
J P
JP
Edson Santos Barbosa
R
R
Ferreira De Albuquerque Junior
R
R
D000328:Adult; D001419:Bacteria / Q000302:isolation & purification*; D018441:Biofilms; D015139:Blotting, Southern / Q000379:methods*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010514:Periodontal Pocket / Q000382:microbiology; D010865:Pilot Projects; D014059:Tongue / Q000382:microbiology*; D014070:Tooth / Q000382:microbiology*
D015342:DNA Probes; D004269:DNA, Bacterial