Generation of multiply charged peptides and proteins by radio frequency acoustic desorption and ionization for mass spectrometric detection.
The design and implementation of a radio frequency acoustic desorption ionization (RADIO) source has been demonstrated for the analysis of multiply charged peptides and proteins. One muL aliquots of melittin, BNP-32, and ubiquitin ( approximately 1 mug of analyte) were deposited onto a quartz crystal microbalance (QCM) electrode before radio frequency actuation for desorption. Continuous electrospray parallel to/above the sampling surface enabled the ionization of desorbed species. Detection by a hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer confirmed the intact and dissociated species observed during MS and MS/MS experiments, respectively.
Dixon
R Brent
RB
W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
Sampson
Jason S
JS
Muddiman
David C
DC
D004566:Electrodes; D004867:Equipment Design; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D020097:Natriuretic Peptide, Brain / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry
D010455:Peptides; D011506:Proteins; D025801:Ubiquitin; D020097:Natriuretic Peptide, Brain; D011791:Quartz