In vitro anti-adhesive activity of green tea extract against pathogen adhesion.
Camellia sinensis polysaccharide has been reported to possess anti-adhesive activity against pathogens. The present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. Green tea extract-4 (CSI-4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. It showed strong inhibitory activities against hemagglutination mediated by pathogens Helicobacter pylori, Propionibacterium acnes and Staphylococcus aureus with the minimum inhibitory concentrations of 0.01-0.5 mg/mL. CSI-4 further demonstrated an inhibitory effect on the adhesion of these pathogens to host cell lines with the IC(50) values (50% inhibition of adhesion) of 0.14-2.3 mg/mL. It exhibited the highest activity against P. acnes, but no inhibitory effects were observed against Lactobacillus acidophilus, Bifidobacterium bifidum, Escherichia coli, or Staphylococcus epidermidis. Our results suggest that CSI-4 may exert a selective anti-adhesive effect against certain pathogenic bacteria with no adverse effects against beneficial or commensal bacteria.
Lee
Ji-Hye
JH
Department of Food Technology, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Shim
Jin Sun
JS
Chung
Mi-Sook
MS
Lim
Seung-Taik
ST
Kim
Kyung Hyun
KH
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D028241:Camellia sinensis / Q000737:chemistry*; D045744:Cell Line, Tumor; D015169:Colony Count, Microbial; D016480:Helicobacter pylori / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D011425:Propionibacterium acnes / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013662:Tea / Q000737:chemistry*
D010936:Plant Extracts; D013662:Tea