Helicobacter pylori IgG antibodies in aqueous humor and serum of subjects with primary open angle and pseudo-exfoliation glaucoma in a South Indian population.
OBJECTIVE
A prospective, nonrandomized, comparative study was carried out to investigate levels of anti-Helicobacter pylori-specific IgG antibodies in the aqueous humor and serum of patients with primary open angle glaucoma (POAG), exfoliation syndrome [pseudo-exfoliation glaucoma (PXFG)], and with normotensive cataract patients, who served as controls.


METHODS
Aqueous humor was aspirated at the beginning of glaucoma surgery from 50 eyes of 50 patients with POAG, with PXFG and at the beginning of phacoemulsification cataract surgery from controls. Serum samples were obtained. Anti-H. pylori IgG concentration in the aqueous humor and serum was measured by means of enzyme linked immunosorbent assay.


RESULTS
Serum analysis of anti-H. pylori IgG antibodies revealed statistically significant difference between POAG and PXFG (52.26+/-52.51 vs. 25.22+/-35.27, P=0.01). Also, the difference between POAG and controls was statistically significant (54.05+/-55.04 vs. 33.83+/-41.73, P=0.04). However, on comparing PXFG with the control group, the difference was statistically insignificant (P=0.12). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in POAG and controls were not statistically different (3.93+/-5.14 vs. 2.65+/-2.87, respectively, P=0.73). The mean concentration of anti-H. pylori IgG antibodies in aqueous humor of patients in PXFG and controls were not statistically different (8.87+/-30.25 vs. 2.65+/-2.87, respectively, P=0.83). There was also no statistical difference of IgG levels between POAG and PXFG (3.93+/-5.14 vs. 8.87+/-30.25, respectively, P=0.87).


CONCLUSIONS
The levels of anti-H. pylori IgG titers in sera of individuals with POAG were significantly higher compared with PXFG and control groups. We support the hypothesis of the role of anti-H. pylori antibodies in causative mechanism for POAG. We could not find a significant link between the anti-H. pylori IgG antibodies and the PXFG.
Deshpande
Nitin
N
Glaucoma Department, Lions Aravind Institute of Community Ophthalmology, Tamil Nadu, India. drnitina@yahoo.com
Lalitha
Prajna
P
Krishna das
S R
SR
Jethani
Jitendra
J
Pillai
R Manju
RM
Robin
Alan
A
Karthik
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D001082:Aqueous Humor / Q000276:immunology*; D016022:Case-Control Studies; D002386:Cataract / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D017889:Exfoliation Syndrome / Q000276:immunology / Q000382:microbiology*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000276:immunology / Q000382:microbiology*; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007194:India; D008297:Male; D008875:Middle Aged; D018918:Phacoemulsification; D011446:Prospective Studies
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G