Prevalence and clinical significance of SEN-H virus in chronic hepatitis B, C and delta infections in Turkey.
OBJECTIVE
SEN viruses are transmitted parenterally and can cause post-transfusion hepatitis. The prevalence and clinical significance of SEN viruses have been investigated in patients with chronic hepatitis C and B but not in D. We aimed to determine the prevalence and clinical significance of SEN viruses- H in patients with chronic hepatitis C, B and delta in Turkey.


METHODS
SEN viruses-H was analyzed in 85 patients with chronic viral hepatitis (30 HCV, 30 HBV and 25 HDV) and 43 non-professional blood donors. HBV DNA, HCV RNA and HDV RNA were positive in patients with hepatitis B, C and D, respectively. SEN viruses-H DNA was detected by semi-nested polymerase chain reaction method (L2AS, C5S primer in first step, L2AS, D11 in second step) after extraction of DNA from sera (NucleoSpin blood; Macherey-Nagel GmbH & Co KG, Germany).


RESULTS
SEN viruses-H DNA was found to be positive in 7/30 (23.3%), 10/30 (33.3%), 6/25 (24%), and 7/43 (16.2%) of patients with chronic C, B, and D hepatitis and healthy blood donors, respectively. There was no significant difference in clinical features and treatment response between SEN viruses- H-positive and -negative patients with chronic viral hepatitis.


CONCLUSIONS
SEN viruses is more frequent in chronic hepatitis patients than in healthy blood donors. These results indicate that SEN viruses has no effect on the clinical course and treatment response of chronic viral hepatitis.
Cakaloğlu
Yilmaz
Y
Department of Gastroenterohepatology, Istanbul Medical Faculty, Istanbul, Turkey. ycoglu@superonline.com
Akyüz
Filiz
F
Bozaci
Mürvet
M
Ibrişim
Duygu
D
Pinarbaşi
Binnur
B
Demir
Kadir
K
Kaymakoğlu
Sabahattin
S
Beşişik
Fatih
F
Badur
Selim
S
Okten
Atilla
A
D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001782:Blood Donors / Q000706:statistics & numerical data; D015897:Comorbidity; D004266:DNA Virus Infections / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D019694:Hepatitis B, Chronic / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D019698:Hepatitis C, Chronic / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D019701:Hepatitis D, Chronic / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D008297:Male; D015995:Prevalence; D012189:Retrospective Studies; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D016896:Treatment Outcome; D014421:Turkey / Q000453:epidemiology; D005723:gamma-Glutamyltransferase / Q000097:blood
D004279:DNA, Viral; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase