title: Determination of artesunate using reversed-phase HPLC at increased temperature and ELSD detection.
abstract: Artesunate (ART) determination can be performed by evaporative light scattering detection with mobile phase composed of CH(3)CN/HCOOH 0.01 M (40:60 v/v; pH 2.85). Evaporative light scattering detection instead of UV detection allowed to improve the sensitivity and the LOD. However, the evaporative light scattering detection response of dihydro-artemisinin appears weaker than for ART, whereas with UV detection the response of ART and dihydroartemisinin seemed similar. Constant analysis time was obtained on using the mobile phase with a flow rate of 0.5 mL/min and column temperature at 60 degrees C instead of 0.7 mL/min at room temperature. This led to less solvent consumption. Moreover, decrease in the flow rate and increase in the column temperature were advantageous for higher sensitivity with both evaporative light scattering detection and UV detection. ART determination in rectal gel and suppositories were compared with these different detection modes and similar results were obtained.
authors.affiliation: PPF-Parasitologie-Pharmacie, Faculté de Pharmacie, Université Bordeaux 2, Bordeaux, France. karen.gaudin@u-bordeaux2.fr
authors.forename: Karen
authors.initials: K
authors.lastname: Gaudin
authors.forename: Tina
authors.initials: T
authors.lastname: Kauss
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lagueny
authors.forename: Pascal
authors.initials: P
authors.lastname: Millet
authors.forename: Fawaz
authors.initials: F
authors.lastname: Fawaz
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Dubost
mesh_terms: D037621:Artemisinins / Q000032:analysis* / Q000737:chemistry*; D000077332:Artesunate; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005782:Gels / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D012996:Solutions; D013696:Temperature; D014835:Volatilization
chemical_list: D037621:Artemisinins; D005782:Gels; D012996:Solutions; D000077332:Artesunate