Allatotropin-like peptide in Malpighian tubules: insect renal tubules as an autonomous endocrine organ.
Malpighian tubules (MTs) are recognised as the main excretory organ in insects, ensuring water and mineral balance. Haematophagous insects incorporate with each meal a large quantity of blood, producing a particularly large volume of urine in a few hours. In the present study, we report the presence of an allatotropin-like (AT-like) peptide in MTs of Triatoma infestans (Klug). The AT-like content in MTs decreased during the first hours after blood-intake, correlating with the post-prandial diuresis. In vivo artificial dilution of haemolymph showed a similar effect. Isolated MTs challenged with a diluted saline solution resulted in an autonomous and reversible response of the organ regulating the quantity of peptide released to the medium, and suggesting that MTs synthesise the AT-like peptide. While MTs are recognised as the target for several hormones, our results corroborate that they also have the ability to produce and secrete a hormone in an autonomous way.
Santini
Maria Soledad
MS
Centro Regional de Estudios Genomicos (CREG), Universidad Nacional de La Plata, Parque Tecnologico Florencio Varela, Buenos Aires, Argentina.
Ronderos
Jorge Rafael
JR
D000818:Animals; D004231:Diuresis; D004703:Endocrine System / Q000502:physiology; D006458:Hemolymph / Q000378:metabolism; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000378:metabolism*; D008317:Malpighian Tubules / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism*; D009997:Osmotic Pressure; D013312:Stress, Physiological; D014224:Triatoma / Q000378:metabolism*
D007301:Insect Hormones; D009479:Neuropeptides; C050669:allatotropin