Diversity of bacterial community in freshwater of Woopo wetland.
Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria.
Baik
Keun Sik
KS
Department of Biology, Sunchon National University, Suncheon, 540-742, Republic of Korea.
Park
Seong Chan
SC
Kim
Eun Mi
EM
Bae
Kyung Sook
KS
Ahn
Jae-Hyung
JH
Ka
Jong-Ok
JO
Chun
Jongsik
J
Seong
Chi Nam
CN
D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001431:Bacteriological Techniques; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D017753:Ecosystem; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D014644:Genetic Variation; D007723:Korea; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053833:Wetlands
D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S