[Programmed application of extracellular matrix promotes neural differentiation of mouse embryonic stem cells].
OBJECTIVE
To study the role of extracellular matrix (ECM) in neural differentiation of mouse embryonic stem cells (ESCs).


METHODS
Mouse ESCs were incubated in the ESC conditioned medium, and the formation of embryonic bodies (EBs) were induced in bacteriological dishes using high-concentration all-trans retinoic acid (RA). The EBs were seeded on different matrixes (gelatin, fibronectin, and laminin/poly-L-ornithine) to test their impact on neural differentiation of the ESCs using immunofluorescence assay. The effect of laminin/poly-L-ornithine on the growth of neurites was evaluated with fluorescence microscopy.


RESULTS
High-concentration RA activated and accelerated the differentiation of ESCs toward nestin-positive neural progenitor cells. Fibronectin supplement in the matrix dose-dependently promoted ESC differentiation into neural progenitor cells, while laminin/poly-L-ornithine increased the growth of the neurites and induced the maturation of the differentiated neural cells.


CONCLUSIONS
ECM plays an important role in neural differentiation of mouse ESCs, and application of FN produces the most conspicuous effect during the differentiation of the ESCs into the neural progenitor cells;laminin/poly-L-ornithine is the most effective during their differentiation into neural cells.
Shan
Zhi-yan
ZY
Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China. shanzhiyan@yahoo.com.cn
Lei
Lei
L
Chen
Ya-jun
YJ
Jin
Lian-hong
LH
Shen
Jing-ling
JL
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D005109:Extracellular Matrix / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D010455:Peptides; C008973:polyornithine; D014212:Tretinoin