The plant phenolic compound p-coumaric acid represses gene expression in the Dickeya dadantii type III secretion system.
The type III secretion system (T3SS) is a major virulence factor in many gram-negative bacterial pathogens. This secretion system translocates effectors directly into the cytosol of eukaryotic host cells, where the effector proteins facilitate bacterial pathogenesis by interfering with host cell signal transduction and other cellular processes. Plants defend themselves against bacterial pathogens by recognizing either the type 3 effectors or their actions and initiating a cascade of defense responses that often results in programmed cell death of the plant cell being attacked. Here we show that a plant phenolic compound, p-coumaric acid (PCA), represses the expression of T3SS genes of the plant pathogen Dickeya dadantii, suggesting that plants can also defend against bacterial pathogens by manipulating the expression of the T3SS. PCA repressed the expression of T3SS regulatory genes through the HrpX/Y two-component system, a core regulator of the T3SS, rather than through the global regulator GacS/A, which indirectly regulates the T3SS. A further analysis of several PCA analogs suggests that the para positioning of the hydroxyl group in the phenyl ring and the double bond of PCA may be important for its biological activity.
Li
Yan
Y
Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211, USA.
Peng
Quan
Q
Selimi
Dija
D
Wang
Qi
Q
Charkowski
Amy O
AO
Chen
Xin
X
Yang
Ching-Hong
CH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D003373:Coumaric Acids / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D026901:Membrane Transport Proteins / Q000096:biosynthesis*; D008954:Models, Biological; D015394:Molecular Structure; D010944:Plants / Q000276:immunology; D011422:Propionates; D037521:Virulence Factors / Q000037:antagonists & inhibitors*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003373:Coumaric Acids; D026901:Membrane Transport Proteins; D011422:Propionates; D037521:Virulence Factors; C495469:p-coumaric acid