Inhibition of the SUMO pathway by Gam1.
We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro. Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.
Pozzebon
Mariaelena
M
FIRC Institute of Molecular Oncology, European Institute of Oncology (IFOM-IEO), Milan, Italy.
Segré
Chiara V
CV
Chiocca
Susanna
S
D000818:Animals; D002478:Cells, Cultured; D019411:Clinical Laboratory Techniques; D006801:Humans; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D025842:SUMO-1 Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D014764:Viral Proteins / Q000302:isolation & purification / Q000494:pharmacology*
C105052:CELO protein, adenovirus; D011994:Recombinant Proteins; D025842:SUMO-1 Protein; D014764:Viral Proteins