Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene.
Insulin receptor substrate-1(IRS-1) gene is one member of the Insulin receptor substrate (IRS) gene family, which plays an important role in mediating the growth of skeletal muscle and the molecular metabolism of type 2 diabetes. Here, we cloned a 3,573 bp fragment of the partial CDS sequence of porcine IRS-1 gene by in silicon cloning strategy and RT-PCR method. The porcine IRS-1 gene was assigned to SSC15q25 by using IMpRH. Sequencing of PCR products from Duroc and Tibetan pig breeds identified one SNP in exon 1 of porcine IRS-1 gene (C3257A polymorphisms). Association analysis of genotypes with the growth traits, anatomy traits, meat quality traits and physiological biochemical indexes traits showed that different genotypes at locus 3,257 of IRS-1 have significant differences in carcass straight length in pigs (P = 0.0102 \ 0.05).
Niu
P-X
PX
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
Huang
Z
Z
Li
C-C
CC
Fan
B
B
Li
K
K
Liu
B
B
Yu
M
M
Zhao
S-H
SH
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D056726:Genetic Association Studies / Q000379:methods*; D005838:Genotype; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D008460:Meat; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics* / Q000254:growth & development; D014018:Tissue Distribution
D055504:Insulin Receptor Substrate Proteins