Cross-priming amplification for rapid detection of Mycobacterium tuberculosis in sputum specimens.
Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear- and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.
Fang
Rendong
R
Department of TB Control, Shanghai Municipal Centers for Disease Control and Prevention, Shanghai 200336, China.
Li
Xia
X
Hu
Lin
L
You
Qimin
Q
Li
Jing
J
Wu
Jie
J
Xu
Peng
P
Zhong
Huayan
H
Luo
Ying
Y
Mei
Jian
J
Gao
Qian
Q
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis*
D017931:DNA Primers