Amino acids of Coxsackie B5 virus are critical for infection of the murine insulinoma cell line, MIN-6.
It was shown recently that 15 successive passages of a laboratory strain of the Coxsackie B virus 5 in a mouse pancreas (CBV-5-MPP) resulted in apparent changes in the virus phenotype, which led to the capacity to induce a diabetes-like syndrome in mice. For further characterization of islet cell interactions with a passaged virus strain, a murine insulinoma cell line, MIN-6, was selected as an experimental model. The CBV-5-MPP virus strain was not able to replicate in MIN-6 cells in vitro but required adaptation over a few days for progeny production and the generation of cytopathic effects. In order to determine the genetic characteristics required for virus growth in MIN-6 cells, the whole genome of the MIN-6-adapted virus variant was sequenced, and critical amino acids were identified by comparing the sequence with that of a virus strain passaged repeatedly in the mouse pancreas. The results of site-directed mutagenesis demonstrated that only one residue, amino acid 94 of VP1, is a major determinant for virus adaptation to MIN-6 cells.
Al-Hello
Haider
H
Enterovirus Laboratory, National Public Health Institute (KTL), Helsinki, Finland.
Ylipaasto
Petri
P
Smura
Teemu
T
Rieder
Elisabeth
E
Hovi
Tapani
T
Roivainen
Merja
M
D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry; D045744:Cell Line, Tumor / Q000821:virology; D003384:Coxsackievirus Infections / Q000821:virology*; D029822:Enterovirus B, Human / Q000235:genetics* / Q000502:physiology*; D016679:Genome, Viral; D007340:Insulinoma / Q000821:virology; D008297:Male; D051379:Mice; D016297:Mutagenesis, Site-Directed; D010179:Pancreas / Q000473:pathology / Q000821:virology; D010195:Pancreatitis / Q000821:virology; D012692:Serial Passage; D014779:Virus Replication / Q000235:genetics
D000596:Amino Acids; D036022:Capsid Proteins