title: Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice.
abstract: A dwarf mutant of rice (Oryza sativa L.) by mutagenesis of ethylene methylsulfonate (EMS) treatment from Nipponbare was identified. The mutant exhibited phenotypes of dwarfism and withered leaf tip (dwl1). Based on the internode length of dwl1, this mutant belongs to the dm type of dwarfing. Analysis of elongation of the second sheath and alpha-amylase activity in endosperm showed that the phenotype caused by dwl1 was insensitive to gibberellin acid treatment. Using a large F2 population derived from a cross between the dwl1 and an indica rice variety, TN1, the DWL1 gene was mapped to the terminal region of the long arm of chromosome 3. Fine-mapping delimited it into a 46 kb physical distance between two STS markers, HL921 and HL944, where 6 open reading frames were predicted. Cloning of DWL1 will contribute to dissecting molecular mechanism that regulates plant height in rice, which will be beneficial to molecular assisted selection of this important trait.
authors.affiliation: State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Liwen
authors.initials: L
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Gao
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D017343:Genes, Plant / Q000235:genetics*; D023281:Genomics; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
chemical_list: D009153:Mutagens; D005020:Ethyl Methanesulfonate