title: Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae.
abstract: Kobuvirus is a new genus in the family Picornaviridae. Two species are currently known: Aichi virus (human kobuvirus) and Bovine kobuvirus (U-1). In this study, the complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus (Kobuvirus/swine/S-1-HUN/2007/Hungary, EU787450) were determined. The structure of the S-1-HUN genome, VPg-5'UTR-leader protein-structural proteins (VP0, VP3, VP1)-non-structural proteins (2A-2C, 3A-3D)-3'UTR-poly(A) tail, was found to be typical of picornavirus. The 8210-nucleotide (nt)-long RNA genome contains a large open reading frame (7467 nt) encoding a potential polyprotein precursor of 2488 amino acids (aa) that has 57/56% and 63/64% nt/aa identity with Aichi virus and U-1, respectively. The 5'UTR contains a hepacivirus/pestivirus-like internal ribosomal entry site (IRES type IV group-B-like) with conserved pseudoknot, II and IIIa-f domains. A tandem repeat (a 30-amino-acid-long motif) was detected in 2B. Thirty-nine (65%) of the 60 fecal samples from pigs under the age of 6 months at the tested farm were positive (the incidence was 90% under the age of 3 weeks). Porcine kobuvirus belongs to a potential new species-the third-in the genus Kobuvirus.
authors.affiliation: Regional Laboratory of Virology, ANTSZ Regional Institute of State Public Health Service, Pécs, Hungary. reuter.gabor@baranya.antsz.hu
authors.forename: Gábor
authors.initials: G
authors.lastname: Reuter
authors.forename: Akos
authors.initials: A
authors.lastname: Boldizsár
authors.forename: Péter
authors.initials: P
authors.lastname: Pankovics
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005243:Feces / Q000821:virology; D023061:Gene Order; D016679:Genome, Viral / Q000235:genetics; D052699:Kobuvirus / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010849:Picornaviridae / Q000145:classification* / Q000235:genetics*; D010850:Picornaviridae Infections / Q000662:veterinary; D012367:RNA, Viral / Q000737:chemistry; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins