title: Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles.
abstract: Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.
authors.affiliation: Department of Virology, College of Biological and Earth Sciences, Sri Venkateswara University, Tirupati 517 502, India. pothursree@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Hema
authors.forename: Ch V
authors.initials: ChV
authors.lastname: Subba Reddy
authors.forename: H S
authors.initials: HS
authors.lastname: Savithri
authors.forename: P
authors.initials: P
authors.lastname: Sreenivasulu
mesh_terms: D002213:Capsid / Q000737:chemistry; D036022:Capsid Proteins / Q000737:chemistry*; D002499:Centrifugation, Density Gradient / Q000379:methods; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D009029:Mosaic Viruses / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D017800:Potyvirus / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry; D014773:Virology / Q000379:methods; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins