title: Anticancer drug-DNA interactions measured using a photoinduced electron-transfer mechanism based on luminescent quantum dots.
abstract: A sensing system based on the photoinduced electron transfer of quantum dots (QDs) was designed to measure the interaction of anticancer drug and DNA, taking mitoxantrone (MTX) as a model drug. MTX adsorbed on the surface of QDs can quench the photoluminescence (PL) of QDs through the photoinduced electron-transfer process; and then the addition of DNA will bring the restoration of QDs PL intensity, as DNA can bind with MTX and remove it from QDs. Sensitive detection of MTX with the detection limit of 10 nmol L(-1) and a linear detection range from 10 nmol L(-1) to 4.5 micromol L(-1) was achieved. The dependence of PL intensity on DNA amount was successfully utilized to investigate the interactions between MTX and DNA. Both the binding constants and the sizes of binding site of MTX-DNA interactions were calculated based on the equations deduced for the PL recovery process. The binding constant obtained in our experiment was generally consistent with previous reports. The sensitive and speedy detection of MTX as well as the avoidance of modification or immobilization process made this system suitable and promising in the drug-DNA interaction studies.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Jipei
authors.initials: J
authors.lastname: Yuan
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.forename: Xiurong
authors.initials: X
authors.lastname: Yang
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D006147:Guanine / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods; D008942:Mitoxantrone / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D009841:Oligonucleotides / Q000737:chemistry; D055668:Photochemical Processes; D045663:Quantum Dots; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013691:Tellurium / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D019187:Cadmium Compounds; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D006147:Guanine; D004247:DNA; D008942:Mitoxantrone; D013691:Tellurium; C028337:cadmium telluride