Probing molecular level interaction of oseltamivir with H5N1-NA and model membranes by molecular docking, multinuclear NMR and DSC methods.
Structure-based drug design has led to the introduction of three drugs--oseltamivir (GS-4104), zanamivir (GG-167) and peramivir (RWJ-270201) which target the enzyme neuraminidase, for treatment of influenza infections. Using comparative docking studies we propose that more potent molecules against neuraminidase can be obtained by appending extra positively charged substituents at the C5 position of the oseltamivir skeleton. This provides an additional interaction with the enzyme and may overcome the problem of resistance encountered with these drugs. To get an insight into the transport and absorption of oseltamivir--the ethyl ester prodrug (GS-4104) as well as its mechanism of action, we have carried out 1H, 13C, 31P NMR, DSC and TEM studies on GS-4104 with model membranes prepared from DMPC/DPPC/POPC. These studies reveal that interactions between GS-4104 and the membrane are both electrostatic (involving H-bonding) and hydrophobic (involving the hydrophobic chain and cyclohexene ring of GS-4104) in nature. The prodrug is seen to increase the fluidity as well as stabilize the bilayer phase of the membrane. This property may be responsible for preventing viral entry into the cells by preventing fusion of the virus outer coat with the cell membrane.
D'Souza
Charlotte
C
Prin K M Kundnani College of Pharmacy, Cuffe Parade, Mumbai-400005, India.
Kanyalkar
Meena
M
Joshi
Mamata
M
Coutinho
Evans
E
Srivastava
Sudha
S
D002152:Calorimetry, Differential Scanning; D053124:Influenza A Virus, H5N1 Subtype / Q000201:enzymology*; D008024:Ligands; D008567:Membranes, Artificial; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D015335:Molecular Probes / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D009439:Neuraminidase / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D053139:Oseltamivir / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
D008024:Ligands; D008567:Membranes, Artificial; D015335:Molecular Probes; D053139:Oseltamivir; D009439:Neuraminidase