title: A micro-potentiometric hemoglobin immunosensor based on electropolymerized polypyrrole-gold nanoparticles composite.
abstract: We report a novel micro-potentiometric hemoglobin (Hb) immunosensor based on electrochemically synthesized polypyrrole (PPy)-gold nanoparticles (AuNPs) composite. PPy-AuNPs film with AuNPs uniformly distributed in it was deposited on gold electrode surface by a simple and direct procedure, without the addition of any nanoparticles or reducing agent. And this generic method makes it possible to deposite different polymers on miniaturized electrodes. With the existence of AuNPs, the antibody immobilization onto the electrode surface was facilitated. Morphology study by field emission scanning electron microscope (FE-SEM) confirms the presence of AuNPs in PPy. Based on an ion-sensitive field-effect transistors (ISFETs) integrated chip, a micro-potentiometric immunosensor for Hb and hemoglobin-A1c (HbA1c) has been constructed. The sensor response was linear over the concentration range 60-180 microg/ml Hb and 4-18 microg/ml HbA1c. The Hb concentration in whole blood samples has also been analysed, with a linear dose-response behavior between 125 and 197 microg/ml and a sensitivity of 0.20 mV microg(-1)ml. The measuring ranges of the developed Hb and HbA1c immunosensors meet the clinical demand for measuring the HbA1c/Hb ratio of 5-20%. This sensor results in simple and rapid differential measurement of Hb and HbA1c, and has great potential to become an inexpensive and portable device for monitoring of diabetes.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, No. 2 Zhong-Guan-Cun-Bei-Er-Jie, Haidian District, Beijing 100190, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Qu
authors.forename: Shanhong
authors.initials: S
authors.lastname: Xia
authors.forename: Chao
authors.initials: C
authors.lastname: Bian
authors.forename: Jizhou
authors.initials: J
authors.lastname: Sun
authors.forename: Jinghong
authors.initials: J
authors.lastname: Han
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation*; D008839:Microelectrodes; D008904:Miniaturization; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D014173:Transistors, Electronic
chemical_list: D006454:Hemoglobins; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006046:Gold