Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles.
We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)(3)(2+) and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)(3)(2+) and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)(3)(2+) film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)(3)(2+) film to promote the electron transfer between Ru(bpy)(3)(2+) and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 microM to 5.2 mM with a detection limit of 12nM.
Deng
Liu
L
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Number 5625, Renmin Road, Changchun, Jilin 130022, PR China.
Zhang
Lihua
L
Shang
Li
L
Guo
Shaojun
S
Wen
Dan
D
Wang
Fuan
F
Dong
Shaojun
S
D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis*; D005782:Gels / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D009243:NAD / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013451:Sulfonic Acids / Q000737:chemistry
D005782:Gels; D013451:Sulfonic Acids; D009243:NAD; D000431:Ethanol; D006046:Gold; D000426:Alcohol Dehydrogenase