Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction.
In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.
Kim
Ekyune
E
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chung-buk, Korea.
Park
Ki-Eun
KE
Kim
Ji-Su
JS
Baek
Dong Chul
DC
Lee
Jae-Woong
JW
Lee
Sang-Rae
SR
Kim
Myeong-Su
MS
Kim
Sang-Hyun
SH
Kim
Chan-Shick
CS
Koo
Deog-Bon
DB
Kang
Han-Seok
HS
Ryoo
Zae-Young
ZY
Chang
Kyu-Tae
KT
D051722:ADAM Proteins / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D015153:Blotting, Western / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D018628:Gene Dosage; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013552:Swine / Q000235:genetics / Q000502:physiology*
D020033:Protein Isoforms; D004247:DNA; D051722:ADAM Proteins