Deciphering soluble and membrane protein function using yeast systems (Review).
Membrane protein-protein interactions are important for regulation, targeting, and activity of proteins in membranes but are difficult to detect and analyse. This review covers current approaches to studying membrane protein interactions. In addition to standard biochemical and genetic techniques, the classic yeast nuclear two-hybrid system has been highly successful in identification and characterization of soluble protein-protein interactions. However, classic yeast two-hybrid assays do not work for membrane proteins because such yeast-based interactions must occur in the nucleus. Here, we highlight recent advances in yeast systems for the detection and characterization of eukaryote membrane protein-protein interactions. We discuss these implications for drug screening and discovery.
Bao
Leyuan
L
Endothelial Cell Biology Unit and Institute of Molecular and Cellular Biology, Leeds Institute of Genetics, Health and Therapeutics, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
Redondo
Clara
C
Findlay
John B C
JB
Walker
John H
JH
Ponnambalam
Sreenivasan
S
D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
D008565:Membrane Proteins; D011506:Proteins