In vivo and in vitro alterations in influenza A/H3N2 virus M2 and hemagglutinin genes: effect of passage in MDCK-SIAT1 cells and conventional MDCK cells.
No mutations were detected in the hemagglutinin gene of influenza A/H3N2 virus isolates from patients undergoing short-term amantadine treatment. However, genetic changes occurred after serial passage in either MDCK or MDCK-SIAT1 cells. Our results showed that only a few mutations were observed in MDCK-SIAT1-passaged isolates in the presence of amantadine.
Li
Danjuan
D
Department of Public Health, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
Saito
Reiko
R
Suzuki
Yasushi
Y
Sato
Isamu
I
Zaraket
Hassan
H
Dapat
Clyde
C
Caperig-Dapat
Isolde Ma
IM
Suzuki
Hiroshi
H
D000818:Animals; D002460:Cell Line; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology; D009154:Mutation; D012692:Serial Passage; D014763:Viral Matrix Proteins / Q000235:genetics*; D000086082:Viroporin Proteins
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins; C483468:hemagglutinin, human influenza A virus