title: Variability in the cadherin gene in an Ostrinia nubilalis strain selected for Cry1Ab resistance.
abstract: Transgenic corn expressing Cry1Ab (a Bacillus thuringiensis toxin) is highly effective in the control of Ostrinia nubilalis. For its toxic action, Cry1Ab has to bind to specific insect midgut proteins. To date, in three Lepidoptera species resistance to a Cry1A toxin has been conferred by mutations in cadherin, a protein of the Lepidoptera midgut membrane. The implication of cadherin in the resistance of an Ostrinia nubilalis colony (Europe-R) selected with Bacillus thuringiensis Cry1Ab protoxin was investigated. Several major mutations in the cadherin (cdh) gene were found, which introduced premature termination codons and/or large deletions (ranging from 1383 to 1701bp). The contribution of these major mutations to the resistance was analyzed in resistant individuals that survived exposure to a high concentration of Cry1Ab protoxin. The results indicated that the presence of major mutations was drastically reduced in individuals that survived exposure. Previous inheritance experiments with the Europe-R strain indicated the involvement of more than one genetic locus and reduced amounts of the cadherin receptor. The results of the present work support a polygenic inheritance of resistance in the Europe-R strain, in which mutations in the cdh gene would contribute to resistance by means of an additive effect.
authors.affiliation: Department of Genetics, University of Valencia, Burjassot, Valencia, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Bel
authors.forename: Herbert A A
authors.initials: HA
authors.lastname: Siqueira
authors.forename: Blair D
authors.initials: BD
authors.lastname: Siegfried
authors.forename: Juan
authors.initials: J
authors.lastname: Ferré
authors.forename: Baltasar
authors.initials: B
authors.lastname: Escriche
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance; D004731:Endotoxins / Q000494:pharmacology*; D014644:Genetic Variation; D006460:Hemolysin Proteins / Q000494:pharmacology*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D009036:Moths / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D009154:Mutation; D011110:Polymorphism, Genetic
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D015820:Cadherins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis