Study of the effect of sample preparation and cooking on the selenium speciation of selenized potatoes by HPLC with ICP-MS and electrospray ionization MS/MS.
The efficiency of enzymatic hydrolysis and leaching with water using accelerated solvent extraction (ASE) or boiling was investigated for quantitative Se speciation in selenized potatoes using reversed phase HPLC coupled to ICP-MS. Preliminary identification of selenomethionine (SeMet), Se-methylselenocysteine (SeMeCys), and selenate in extracts of potato skin and flesh was achieved using complementary reversed phase and anion-exchange HPLC-ICP-MS and retention time matching with standards. The quantitative speciation data revealed a higher percentage of selenomethionine (73% of the total Se) found in the flesh in comparison with skin (containing 21% of the total Se as SeMet). ASE and boiling in water were found to be similar in terms of Se extraction efficiency and profiles. However, ASE was found to be more efficient than boiling with respect to sample cleanup and reduced sample handling. The presence of SeMet at parts per billion levels in selenized potatoes was confirmed by reversed phase HPLC with online ESI MS/MS.
Infante
Heidi Goenaga
HG
LGC Limited, Queens Road, Teddington, Middlesex, United Kingdom. Heidi.Goenaga-Infante@lgc.co.uk
Borrego
Ana Arias
AA
Peachey
Emma
E
Hearn
Ruth
R
O'Connor
Gavin
G
Barrera
Tamara García
TG
Ariza
José Luis Gómez
JL
D002851:Chromatography, High Pressure Liquid; D005527:Food, Fortified / Q000032:analysis; D006358:Hot Temperature; D011492:Protein Hydrolysates; D012643:Selenium / Q000008:administration & dosage* / Q000032:analysis; D018036:Selenium Compounds / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D011492:Protein Hydrolysates; D018036:Selenium Compounds; C007978:hydrolysin; D012643:Selenium