Structure of the O-antigen of Yersinia pseudotuberculosis O:4a revised.
The O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Yersinia pseudotuberculosis O:4a and studied by NMR spectroscopy, including 2D ROESY and (1)H, (13)C HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established, which differs from the structure reported earlier [Gorshkova, R. P. et al., Bioorg. Khim. 1983, 9, 1401-1407] in the linkage modes between the monosaccharides: [carbohydrate sequence see in text] where Tyv stands for 3,6-dideoxy-D-arabino-hexose (tyvelose). The structure of the Y. pseudotuberculosis O:4a antigen resembles that of Y. pseudotuberculosis O:2c, which differs in the presence of abequose (3,6-dideoxy-D-xylo-hexose) in place of tyvelose only.
Kondakova
Anna N
AN
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation. annakond@gmail.com
Bystrova
Olga V
OV
Shaikhutdinova
Rima Z
RZ
Ivanov
Sergey A
SA
Dentovskaya
Svetlana V
SV
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Anisimov
Andrey P
AP
D002240:Carbohydrate Sequence; D006601:Hexoses / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D015011:Yersinia pseudotuberculosis / Q000737:chemistry*
D006601:Hexoses; D019081:O Antigens; C009616:tyvelose