title: Mobilization of bone marrow-derived Oct-4+ SSEA-4+ very small embryonic-like stem cells in patients with acute myocardial infarction.
abstract: OBJECTIVE
  This study sought to assess of the mobilization of nonhematopoietic very small embryonic-like stem cells (VSELs) in acute myocardial infarction (MI).
  
  
  BACKGROUND
  Acute MI induces mobilization of bone marrow stem cells. Recently, a rare population of VSELs, expressing markers of embryonic pluripotent stem cells (PSCs), was identified in adult murine bone marrow and human umbilical cord blood.
  
  
  METHODS
  Thirty-one patients with acute MI and 30 healthy subjects were enrolled. Blood was sampled on admission, after 24 h, and 5 days later. Erythrocytes were lysed and lin(-)CD45(-) VSELs were isolated using a live cell sorting system (FACSAria, Beckton Dickinson, San Jose, California).
  
  
  RESULTS
  In healthy subjects the median number of circulating VSELs was very low (median 0.8 [range 0 to 1.3]) cells/microl. In acute MI, VSELs were mobilized early (median 2.7 [range 0.2 to 3.9] cells/microl; p < 0.001) and remained elevated after 24 h and 5 days (median 4.7 [range 0.2 to 6.4] cells/microl; p < 0.003, and median 2.6 [range 0.3 to 3.6] cells/microl; p < 0.03, respectively). The mobilization of VSEL was significantly reduced in patients older than 50 years and with diabetes in comparison with younger and nondiabetic patients. Circulating VSELs were small (7 to 8 microm) and enriched in the messenger ribonucleic acid of PSC markers (Oct-4, Nanog), cardiac lineage (GATA-4, Nkx2.5/Csx, MEF2C), and endothelial (VE-cadherin) markers. The presence of PSC markers (Oct-4, SSEA-4) and the chemokine receptor CXCR4 in circulating VSELs was confirmed at the protein level by immunofluorescent staining and ImageStream system (Amnis Corporation, Seattle, Washington) analysis.
  
  
  CONCLUSIONS
  Acute MI induced mobilization of VSELs expressing pluripotent markers, early cardiac and endothelial markers, and chemokine receptor CXCR4.
authors.affiliation: Third Division of Cardiology, Medical University of Silesia, Katowice, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wojakowski
authors.forename: Michał
authors.initials: M
authors.lastname: Tendera
authors.forename: Magda
authors.initials: M
authors.lastname: Kucia
authors.forename: Ewa
authors.initials: E
authors.lastname: Zuba-Surma
authors.forename: Edyta
authors.initials: E
authors.lastname: Paczkowska
authors.forename: Joanna
authors.initials: J
authors.lastname: Ciosek
authors.forename: Maciej
authors.initials: M
authors.lastname: Hałasa
authors.forename: Marek
authors.initials: M
authors.lastname: Król
authors.forename: Maciej
authors.initials: M
authors.lastname: Kazmierski
authors.forename: Paweł
authors.initials: P
authors.lastname: Buszman
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ochała
authors.forename: Janina
authors.initials: J
authors.lastname: Ratajczak
authors.forename: Bogusław
authors.initials: B
authors.lastname: Machaliński
authors.forename: Mariusz Z
authors.initials: MZ
authors.lastname: Ratajczak
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D016022:Case-Control Studies; D019070:Cell Lineage; D002469:Cell Separation; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D005312:Fetal Blood; D015870:Gene Expression; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000473:pathology*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology*; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism
chemical_list: D015415:Biomarkers; D050814:Octamer Transcription Factor-3; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4