Nanoporous cerium oxide thin film for glucose biosensor.
Nanoporous cerium oxide (CeO(2)) thin film deposited onto platinum (Pt) coated glass plate using pulsed laser deposition (PLD) has been utilized for immobilization of glucose oxidase (GOx). Atomic force microscopy studies reveal the formation of nanoporous surface morphology of CeO(2) thin film. Response studies carried out using differential pulsed voltammetry (DPV) and optical measurements show that the GOx/CeO(2)/Pt bio-electrode shows linearity in the range of 25-300 mg/dl of glucose concentration. The low value of Michaelis-Menten constant (1.01 mM) indicates enhanced enzyme affinity of GOx to glucose. The observed results show promising application of the nanoporous CeO(2) thin film for glucose sensing application without any surface functionalization or mediator.
Saha
Shibu
S
Department of Physics & Astrophysics, University of Delhi, Delhi 110007, India.
Arya
Sunil K
SK
Singh
S P
SP
Sreenivas
K
K
Malhotra
B D
BD
Gupta
Vinay
V
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D002563:Cerium / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D008567:Membranes, Artificial; D002563:Cerium; C030583:ceric oxide; D005949:Glucose Oxidase; D005947:Glucose