title: Cost-effective method for growing three-dimensional cell cultures in extracellular matrix extract.
abstract: Three-dimensional (3-D) cell cultures are advantageous for modeling complex cellular behavior. A major issue with 3-D cultures grown in Engelbreth-Holm-Swarm (EHS) extracellular matrix extract, however, is the expense. We describe here a simple method using a rubber gasket laid over a coverslip, which provides sufficient 3-D area filled with a minimal amount of EHS, resulting in cost-effective 3-D cellular cultures that are easy to manipulate.
authors.affiliation: Genomic Medicine Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
authors.forename: A I
authors.initials: AI
authors.lastname: Jacob
authors.forename: K A
authors.initials: KA
authors.lastname: Waite
authors.forename: C
authors.initials: C
authors.lastname: Eng
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000191:economics / Q000379:methods*; D002460:Cell Line; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry; D005109:Extracellular Matrix
chemical_list: D003470:Culture Media