title: Efficacy of biofilm disclosing agent and of three brushes in the control of complete denture cleansing.
abstract: OBJECTIVE
  This report evaluated the efficacy of three brushes and one biofilm disclosing agent in complete denture cleansing.
  
  
  METHODS
  Twenty-seven wearers of maxillary dentures were distributed into three groups and received different brushes: Oral B40, conventional toothbrush (Oral B); Denture, denture-specific brush (Condor); Johnson & Johnson, denture-specific brush (Johnson & Johnson). The 60-day experimental period was divided into two techniques: I - brushing (brush associated with a paste - Dentu Creme, Dentco) three times a day; II - brushing and daily application of 1% neutral red on the denture internal surface. Biofilm quantification was carried out weekly and the areas with dye biofilm were obtained by means of Image Tool 2.02 software.
  
  
  RESULTS
  Biofilm removal was more effective during Technique II (Wilcoxon test: p=0.01) for the three groups of brushes. When the brushes were compared in Technique I, the Kruskal Wallis test indicated statistical difference between Denture X Johnson & Johnson and Denture X Oral B40, in which the Denture was more efficient. For Technique II, there was no statistical difference between brushes (p>0.05).
  
  
  CONCLUSIONS
  The disclosed application promoted more efficacy on biofilm removal, regardless of the brush used. Denture (Condor) was more efficient than the other brushes during Technique I.
authors.affiliation: Department of Dental Materials and Prosthodontics, Ribeirão Preto Dental School, University of São Paulo, Brazil.
authors.forename: Cláudia Helena Lovato
authors.initials: CH
authors.lastname: da Silva
authors.forename: Helena de Freitas Oliveira
authors.initials: Hde F
authors.lastname: Paranhos