title: Aberrant expression of ID2 protein and its correlation with EBV-LMP1 and P16(INK4A) in classical Hodgkin lymphoma in China.
abstract: BACKGROUND
  The relationships between the expression of ID2, EBV-LMP1 and P16(INK4A) in Chinese classical Hodgkin lymphoma are unknown and need exploring.
  
  
  METHODS
  Samples of classical Hodgkin lymphoma from 60 Chinese patients were analyzed for the expression of ID2, EBV-LMP1 and p16(INK4A) proteins by immunohistochemistry.
  
  
  RESULTS
  ID2 protein was expressed in 83.3% of this group of classical Hodgkin lymphoma, staining strongly in both cytoplasm and nucleus of the Hodgkin and Reed-Sternberg (HRS) cells. EBV-LMP1 and P16(INK4A) were overexpressed in 85.0% and 71.7% of Hodgkin lymphoma, respectively. EBV-LMP1 was noted in the cytoplasm, membrane and nucleus of HRS cells; P16(INK4A) was in the nucleus and cytoplasm. Microscopically, ID2, EBV-LMP1 and P16(INK4A) staining distinguished the HRS cells from the complex background of lymphocytes. ID2 was positively correlated with EBV-LMP1(P < 0.01), but P16(INK4A) was inversely related to EBV-LMP1 (P < 0.05).
  
  
  CONCLUSIONS
  It is suggested that ID2, EBV-LMP1 and P16(INK4A) could play an important role in the evolution of classical Hodgkin lymphoma, and be considered as potential adjunct markers to identify HRS cells in diagnosis.
authors.affiliation: Department of Pathology, Chinese People's Liberation Army (PLA) General Hospital, Beijing, PR China. zhaopo@301hospital.com.cn
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Yali
authors.initials: Y
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002681:China; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D051797:Inhibitor of Differentiation Protein 2 / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D016539:Reed-Sternberg Cells / Q000378:metabolism / Q000473:pathology; D014763:Viral Matrix Proteins / Q000378:metabolism*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; C037662:EBV-associated membrane antigen, Epstein-Barr virus; C497450:ID2 protein, human; D051797:Inhibitor of Differentiation Protein 2; D014763:Viral Matrix Proteins