title: Bisphosphonate determination using 1H-NMR spectroscopy for biomedical applications.
abstract: Bisphosphonate is known to be a very active drug in the treatment of osteoporosis and bone regeneration. A new method has been developed, utilizing nuclear magnetic resonance spectroscopy to identify and measure the amount of bisphosphonate in solution. A standard reference with similar functional group to that of the bisphosphonate was chosen and applied in the experimentation. The results showed that the use of nuclear magnetic resonance spectroscopy ((1)H-NMR) in determining the solvent residues of various pharmaceutical drugs has proved to be effective. Unlike chromatography, it is possible to use a universal reference standard as an internal standard assayed by quantitative NMR. Using the same theory, this method is capable of both identifying and quantifying the bisphosphonate in various solutions. This paper is the first publication showing this unique measurement method, which can be used in a range of pharmaceutical and biomedical applications.
authors.affiliation: Department of Physics and Advanced Materials, University of Technology, Sydney, NSW, Australia. joshua.chou@uts.edu.au
authors.forename: J
authors.initials: J
authors.lastname: Chou
authors.forename: R
authors.initials: R
authors.lastname: Shimmon
authors.forename: B
authors.initials: B
authors.lastname: Ben-Nissan
mesh_terms: D004164:Diphosphonates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012015:Reference Standards
chemical_list: D004164:Diphosphonates