The in vivo degradation of a ruthenium labelled polysaccharide-based hydrogel for bone tissue engineering.
In this paper we report a new method that permitted for the first time to selectively track a polysaccharide-based hydrogel on bone tissue explants, several weeks after its implantation. The hydrogel, which was developed for bone healing and tissue engineering, was labelled with a ruthenium complex and implanted into rabbit bone defects in order to investigate its in vivo degradation. 1, 2, 3 and 8 weeks after surgery, the bone explants were analyzed by synchrotron X-ray microfluorescence, infrared mapping spectroscopy, scanning electron microscopy, and optical microscopy after histological coloration. The results showed that the labelled polysaccharide-based hydrogel was likely to undergo phagocytosis that seemed to occur from the edge to the center of the implantation site up to at least the 8th week.
Laïb
Samia
S
INSERM, UMRS 791, Université de Nantes, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, Faculté de chirurgie dentaire, 1 Place Alexis Ricordeau, Nantes Cedex 1, France.
Fellah
Borhane H
BH
Fatimi
Ahmed
A
Quillard
Sophie
S
Vinatier
Claire
C
Gauthier
Olivier
O
Janvier
Pascal
P
Petit
Marc
M
Bujoli
Bruno
B
Bohic
Sylvain
S
Weiss
Pierre
P
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002130:Calcium Phosphates / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000378:metabolism; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005269:Femur / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000378:metabolism*; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D019919:Prosthesis Implantation; D011817:Rabbits; D012428:Ruthenium / Q000378:metabolism*; D013997:Time Factors; D023822:Tissue Engineering
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D065347:Hypromellose Derivatives; D012428:Ruthenium; D008747:Methylcellulose; C020243:calcium phosphate; D019158:N-Acetylneuraminic Acid