title: A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii.
abstract: We have found the transcript of one of at least six ferredoxin encoding genes of the green alga Chlamydomonas reinhardtii, FDX5, strongly accumulating in anaerobiosis, indicating a vital role of the encoded protein in the anaerobic metabolism of the cells. According to absorption and electron paramagnetic resonance spectroscopy, Fdx5 is a plant-type [2Fe2S]-ferredoxin with a redox potential similar to that of the ferredoxin PetF. However, although Fdx5 seems to be located in the chloroplast, it is not able to photoreduce nicotinamide adenine dinucleotide phosphate (NADP(+)) via ferredoxin-NADP-reductase, nor to be an electron donor to the plastidic [FeFe]-hydrogenase HydA1. Thus, Fdx5 seems to have a special role in a yet to be identified anaerobic pathway.
authors.affiliation: Ruhr Universität Bochum, Fakultät für Biologie und Biotechnologie, Lehrstuhl für Biochemie der Pflanzen, AG Photobiotechnologie, ND2/169, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Jessica
authors.initials: J
authors.lastname: Jacobs
authors.forename: Susanne
authors.initials: S
authors.lastname: Pudollek
authors.forename: Anja
authors.initials: A
authors.lastname: Hemschemeier
authors.forename: Thomas
authors.initials: T
authors.lastname: Happe
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: C081714:2Fe-2S ferredoxin; D005288:Ferredoxins; D012333:RNA, Messenger; D010088:Oxidoreductases; C029746:uptake hydrogenase; D005287:Ferredoxin-NADP Reductase