title: Crystal structure of a major outer membrane protein from Thermus thermophilus HB27.
abstract: The thermophilic eubacterium Thermus thermophilus belongs to one of the oldest branches of evolution and has a multilayered cell envelope that differs from that of modern Gram-negative bacteria. Its outer membrane contains integral outer membrane proteins (OMPs), of which only a few are characterized. TtoA, a new beta-barrel OMP, was identified by searching the genome sequence of strain HB27 for the presence of a C-terminal signature sequence. The structure of TtoA was determined to a resolution of 2.8 A, representing the first crystal structure of an OMP from a thermophilic bacterium. TtoA consists of an eight-stranded beta-barrel with a large extracellular part to which a divalent cation is bound. A five-stranded extracellular beta-sheet protrudes out of the membrane-embedded transmembrane barrel and is stabilized by a disulfide bridge. The edge of this beta-sheet forms crystal contacts that could mimic interactions with other proteins. In modern Gram-negative bacteria, the C-terminal signature sequence of OMPs is required for binding to an Omp85 family protein as a prerequisite for its assembly. We present hints that a similar assembly pathway exists in T. thermophilus by an in vitro binding assay, where unfolded TtoA binds to the Thermus Omp85 family protein TtOmp85, while a mutant without the signature sequence does not.
authors.affiliation: Department of Biology, University of Konstanz, Konstanz, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Brosig
authors.forename: Jutta
authors.initials: J
authors.lastname: Nesper
authors.forename: Winfried
authors.initials: W
authors.lastname: Boos
authors.forename: Wolfram
authors.initials: W
authors.lastname: Welte
authors.forename: Kay
authors.initials: K
authors.lastname: Diederichs
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002413:Cations, Divalent / Q000737:chemistry; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D016963:Thermus thermophilus / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002413:Cations, Divalent