[Fabrication and characteristics of oriental poly (lactic-co-glycolic acid) scaffold: a preliminary study].
OBJECTIVE
To explore the method of fabricating oriental scaffolds and investigate the biocompatibility of the scaffolds as well as cells distribution within the scaffolds in vitro.


METHODS
The oriental poly (lactic-co-glycolic acid) (PLGA) scaffolds were fabricated with modified emulsion-phase separation method. The scaffolds were treated with plasma and then anchored with collagen I. Articular chondrocytes were loaded into the scaffolds. The growth status and distributing characteristic of the cells were investigated by environmental scanning electron microscope.


RESULTS
The scaffold was well compatible with the articular chondrocytes. The cells could reach to 2.5 mm depth with unilateral loading. The cells distributed evenly in the scaffold and lined along the inner pipes.


CONCLUSIONS
The oriental scaffold fabricated could significantly promote the distributing characteristics of the chondrocytes. The vertical alignment of the chondrocytes within the scaffold is closely similar to that of articular cartilage.
Yu
Fang-yuan
FY
Orthopedics Institute, General Hospital of People's Liberation Army, Beijing 100853, China.
Lu
Shi-bi
SB
Wang
Shen-guo
SG
Zhao
Bin
B
Bei
Jian-zhong
JZ
Xu
Wen-jing
WJ
Yuan
Mei
M
D002358:Cartilage, Articular / Q000166:cytology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D006016:Glycolates; D006801:Humans; D019344:Lactic Acid; D008422:Materials Testing; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D054457:Tissue Scaffolds
D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid