Autonomic nervous system regulates secretion of anti-inflammatory prohormone SMR1 from rat salivary glands.
The autonomic nervous system regulates the secretion of bioactive proteins and peptides from salivary glands that can be important in systemic physiological responses. The prohormone submandibular rat-1, which is highly expressed in rat submandibular glands, can be cleaved to produce polypeptides with analgesic and anti-inflammatory activities. Human genes related to submandibular rat-1 have conserved biological functions and are potentially important in pain suppression, erectile function, and inflammation. In this study we describe the differential expression and posttranslational modification of submandibular rat-1 protein in salivary glands, the urogenital tract, lung, blood, and saliva in male Sprague-Dawley and Brown Norway rats. Submandibular rat-1 protein is secreted into saliva after the administration of beta-adrenergic or cholinergic agonists. Removal of the sympathetic ganglion that innervates the salivary glands results in increased levels of submandibular rat-1 protein in salivary glands. The secretion of submandibular rat-1 in response to physiological stress may provide a large pool of submandibular rat-1-derived peptide products that can promote analgesia and decrease inflammation locally and systemically. This pathway may be conserved among mammals and may constitute an important anti-inflammatory and analgesic response to stress.
Morris
K E
KE
550A Heritage Medical Research Ctr., Pulmonary Research Group, Dept. of Medicine, Faculty of Medicine and Dentistry, Univ. of Alberta, Edmonton, AB, Canada T6G 2S2.
St Laurent
C D
CD
Hoeve
R S
RS
Forsythe
P
P
Suresh
M R
MR
Mathison
R D
RD
Befus
A D
AD
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001341:Autonomic Nervous System / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D018679:Cholinergic Agonists / Q000494:pharmacology; D005260:Female; D015171:Ganglionectomy; D006031:Glycosylation; D007249:Inflammation / Q000503:physiopathology / Q000517:prevention & control; D008168:Lung / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000378:metabolism; D011498:Protein Precursors / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D011914:Rats, Inbred BN; D017207:Rats, Sprague-Dawley; D012469:Salivary Glands / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012472:Salivation / Q000187:drug effects; D013997:Time Factors; D014566:Urogenital System / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D000906:Antibodies; D018679:Cholinergic Agonists; D011498:Protein Precursors; D012471:Salivary Proteins and Peptides; C066861:Smr3b protein, rat