A tetracycline repressor-based mammalian two-hybrid system to detect protein-protein interactions in vivo.
A mammalian two-hybrid system (termed as trM2H) was developed to detect protein-protein interactions in vivo, based on the reconstitution of the functions the of tetracycline repressor (TetR). The system is sensitive enough to detect protein-protein interactions with K(d) up to 55microM in mammalian cells, and the system can be regulated by small molecules. This system can be used as an efficient genetic selection system to map protein-protein interactions in mammalian cells.
Thibodeaux
Gabrielle Nina
GN
College of Pharmacy, The University of Texas at Austin, 78712-0128, USA.
Cowmeadow
Roshani
R
Umeda
Aiko
A
Zhang
Zhiwen
Z
D000818:Animals; D006801:Humans; D008722:Methods; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D012107:Research Design; D013752:Tetracycline; D020798:Two-Hybrid System Techniques
D013752:Tetracycline