Identification of novel candidate protein biomarkers for the post-polio syndrome - implications for diagnosis, neurodegeneration and neuroinflammation.
Survivors of poliomyelitis often develop increased or new symptoms decades after the acute infection, a condition known as post-polio syndrome (PPS). The condition affects 20-60% of previous polio patients, making it one of the most common causes of neurological deficits worldwide. The underlying pathogenesis is not fully understood and accurate diagnosis is not feasible. Herein we investigated whether it was possible to identify proteomic profile aberrations in the cerebrospinal fluid (CSF) of PPS patients. CSF from 15 patients with well-defined PPS were analyzed for protein expression profiles. The results were compared to data obtained from nine healthy controls and 34 patients with other non-inflammatory diseases which served as negative controls. In addition, 17 samples from persons with secondary progressive multiple sclerosis (SPMS) were added as relevant age-matched references for the PPS samples. The CSF of persons with PPS displayed a disease-specific and highly predictive (p=0.0017) differential expression of five distinct proteins: gelsolin, hemopexin, peptidylglycine alpha-amidating monooxygenase, glutathione synthetase and kallikrein 6, respectively, in comparison with the control groups. An independent ELISA confirmed the increase of kallikrein 6. We suggest that these five proteins should be further evaluated as candidate biomarkers for the diagnosis and development of new therapies for PPS patients.
Gonzalez
Henrik
H
Department of Clinical Sciences, Danderyd Hospital, Karolinska Institute, Stockholm, Sweden.
Ottervald
Jan
J
Nilsson
Kerstin C
KC
Sjögren
Niclas
N
Miliotis
Tasso
T
Von Bahr
Helena
H
Khademi
Mohsen
M
Eriksson
Bodil
B
Kjellström
Sven
S
Vegvari
Akos
A
Harris
Robert
R
Marko-Varga
György
G
Borg
Kristian
K
Nilsson
Johan
J
Laurell
Thomas
T
Olsson
Tomas
T
Franzén
Bo
B
D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D016262:Postpoliomyelitis Syndrome / Q000134:cerebrospinal fluid*; D020543:Proteome / Q000378:metabolism*
D015415:Biomarkers; D020543:Proteome