Immunosuppressive activity of the ethanol extract of Sedum sarmentosum and its fractions on specific antibody and cellular responses to ovalbumin in mice.
The immunosuppressive activity of the ethanol extract of Sedum sarmentosum (EESS) and its fractions was studied with respect to specific antibody and cellular response to ovalbumin (OVA) in mice. ICR Mice were immunized subcutaneously with OVA on days 0 and 14. Beginning on the day of immunization, the mice were administered intraperitoneally (ip) with EESS and it fractions at a single dose of 0.25, 0.5, and 1.0 mg, and cyclosporin A at a single dose of 0.1 mg at intervals of 7 days. On day 28, splenocyte proliferation and specific antibody level in serum were measured. EESS significantly suppressed concanavalin A (Con A)-, lipopolysaccharide (LPS)-, and OVA-induced splenocyte proliferation in the immunized mice in a dose-dependent manner. The OVA-specific serum IgG, IgG1, and IgG2b levels in the immunized mice were also markedly reduced by EESS. Among four fractions of EESS, the BuOH fraction consisting mainly of flavonoid glycosides showed the highest suppressive activity. The results suggest that EESS could suppress the cellular and humoral immune response in mice, and deserve further research to be developed as immunosuppressant.
Qin
Feng
F
College of Animal Sciences, Zhejiang University, Hangzhou, P. R. China.
Sun
Hong-Xiang
HX
D000818:Animals; D049109:Cell Proliferation; D029627:Crassulaceae / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007166:Immunosuppressive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008934:Mitogens / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D008934:Mitogens; D010936:Plant Extracts; D000431:Ethanol; D010047:Ovalbumin