title: The bacterial fermentation product butyrate influences epithelial signaling via reactive oxygen species-mediated changes in cullin-1 neddylation.
abstract: The human enteric flora plays a significant role in intestinal health and disease. Populations of enteric bacteria can inhibit the NF-kappaB pathway by blockade of IkappaB-alpha ubiquitination, a process catalyzed by the E3-SCF(beta-TrCP) ubiquitin ligase. The activity of this ubiquitin ligase is regulated via covalent modification of the Cullin-1 subunit by the ubiquitin-like protein NEDD8. We previously reported that interaction of viable commensal bacteria with mammalian intestinal epithelial cells resulted in a rapid and reversible generation of reactive oxygen species (ROS) that modulated neddylation of Cullin-1 and resulted in suppressive effects on the NF-kappaB pathway. Herein, we demonstrate that butyrate and other short chain fatty acids supplemented to model human intestinal epithelia in vitro and human tissue ex vivo results in loss of neddylated Cul-1 and show that physiological concentrations of butyrate modulate the ubiquitination and degradation of a target of the E3- SCF(beta-TrCP) ubiquitin ligase, the NF-kappaB inhibitor IkappaB-alpha. Mechanistically, we show that physiological concentrations of butyrate induces reactive oxygen species that transiently alters the intracellular redox balance and results in inactivation of the NEDD8-conjugating enzyme Ubc12 in a manner similar to effects mediated by viable bacteria. Because the normal flora produces significant amounts of butyrate and other short chain fatty acids, these data provide a functional link between a natural product of the intestinal normal flora and important epithelial inflammatory and proliferative signaling pathways.
authors.affiliation: Department of Pathology and Laboratory Medicine, Epithelial Pathobiology Unit, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Kumar
authors.forename: Huixia
authors.initials: H
authors.lastname: Wu
authors.forename: Lauren S
authors.initials: LS
authors.lastname: Collier-Hyams
authors.forename: Young-Man
authors.initials: YM
authors.lastname: Kwon
authors.forename: Jason M
authors.initials: JM
authors.lastname: Hanson
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Neish
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D002087:Butyrates / Q000494:pharmacology*; D018938:Caco-2 Cells; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D044842:Cullin Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005285:Fermentation; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D000075687:NEDD8 Protein; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology; D054875:Ubiquitination / Q000187:drug effects; D014452:Ubiquitins / Q000378:metabolism*
chemical_list: D002087:Butyrates; C100318:Cullin 1; D044842:Cullin Proteins; D020672:I-kappa B Proteins; D000075687:NEDD8 Protein; C082860:NEDD8 protein, human; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D017382:Reactive Oxygen Species; D014452:Ubiquitins; D000072000:NF-KappaB Inhibitor alpha