title: Compact and low-cost biosensor based on novel approach to spectroscopy of surface plasmons.
abstract: A high-performance surface plasmon resonance (SPR) sensor based on a novel approach to spectroscopy of surface plasmons is reported. This approach employs a special diffraction grating structure (referred to as surface plasmon resonance coupler and disperser, SPRCD) which simultaneously couples light into a surface plasmon and disperses the diffracted light for spectral readout of SPR signal. The developed SPRCD sensor consists of a miniature cartridge integrating the diffraction grating and microfluidics and a compact optical system which simultaneously acquires data from four independent sensing channels in the cartridge. It is demonstrated that the SPRCD sensor is able to measure bulk refractive index changes as small as 3 x 10(-7) RIU (refractive index units) and to detect short oligonucleotides in concentrations down to 200 pM.
authors.affiliation: Institute of Photonics and Electronics ASCR, Chaberská 57, CZ-18251 Prague, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Piliarik
authors.forename: Milan
authors.initials: M
authors.lastname: Vala
authors.forename: Ivo
authors.initials: I
authors.lastname: Tichý
authors.forename: Jirí
authors.initials: J
authors.lastname: Homola
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D009841:Oligonucleotides / Q000032:analysis* / Q000235:genetics*; D013057:Spectrum Analysis / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D009841:Oligonucleotides