title: Prevalence and clinical significance of SEN-H virus in chronic hepatitis B, C and delta infections in Turkey.
abstract: OBJECTIVE
  SEN viruses are transmitted parenterally and can cause post-transfusion hepatitis. The prevalence and clinical significance of SEN viruses have been investigated in patients with chronic hepatitis C and B but not in D. We aimed to determine the prevalence and clinical significance of SEN viruses- H in patients with chronic hepatitis C, B and delta in Turkey.
  
  
  METHODS
  SEN viruses-H was analyzed in 85 patients with chronic viral hepatitis (30 HCV, 30 HBV and 25 HDV) and 43 non-professional blood donors. HBV DNA, HCV RNA and HDV RNA were positive in patients with hepatitis B, C and D, respectively. SEN viruses-H DNA was detected by semi-nested polymerase chain reaction method (L2AS, C5S primer in first step, L2AS, D11 in second step) after extraction of DNA from sera (NucleoSpin blood; Macherey-Nagel GmbH & Co KG, Germany).
  
  
  RESULTS
  SEN viruses-H DNA was found to be positive in 7/30 (23.3%), 10/30 (33.3%), 6/25 (24%), and 7/43 (16.2%) of patients with chronic C, B, and D hepatitis and healthy blood donors, respectively. There was no significant difference in clinical features and treatment response between SEN viruses- H-positive and -negative patients with chronic viral hepatitis.
  
  
  CONCLUSIONS
  SEN viruses is more frequent in chronic hepatitis patients than in healthy blood donors. These results indicate that SEN viruses has no effect on the clinical course and treatment response of chronic viral hepatitis.
authors.affiliation: Department of Gastroenterohepatology, Istanbul Medical Faculty, Istanbul, Turkey. ycoglu@superonline.com
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Cakaloğlu
authors.forename: Filiz
authors.initials: F
authors.lastname: Akyüz
authors.forename: Mürvet
authors.initials: M
authors.lastname: Bozaci
authors.forename: Duygu
authors.initials: D
authors.lastname: Ibrişim
authors.forename: Binnur
authors.initials: B
authors.lastname: Pinarbaşi
authors.forename: Kadir
authors.initials: K
authors.lastname: Demir
authors.forename: Sabahattin
authors.initials: S
authors.lastname: Kaymakoğlu
authors.forename: Fatih
authors.initials: F
authors.lastname: Beşişik
authors.forename: Selim
authors.initials: S
authors.lastname: Badur
authors.forename: Atilla
authors.initials: A
authors.lastname: Okten
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001782:Blood Donors / Q000706:statistics & numerical data; D015897:Comorbidity; D004266:DNA Virus Infections / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D019694:Hepatitis B, Chronic / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D019698:Hepatitis C, Chronic / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D019701:Hepatitis D, Chronic / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D008297:Male; D015995:Prevalence; D012189:Retrospective Studies; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D016896:Treatment Outcome; D014421:Turkey / Q000453:epidemiology; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D004279:DNA, Viral; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase