[Detection of chromosome 13 deletion in plasma cell leukemia by dual-color fluorescence in situ hybridization].
This study was aimed to investigate the deletion features of chromosome 13 in plasma cell leukemia (PCL) and its relationship with clinical features. The dual-color fluorescence in situ hybridization (FISH) with two different specific probes for the regions containing 13q14 and 13q31 was used to delet the chromosome in 21 patients with PCL. The results showed that 13 (61.9%) out of 21 PCL had 13q14 deletion. The number of 13q14 deletion cells ranged from 52% to 98%. 12 patients (57.4%) had 13q31 deletion. The number of cells with 13q31 deletion ranged from 50% to 98%. Out of 13 cases with (13q14) deletion, 12 cases had (13q31) deletion, but only 1 out of 12 cases was negative. It is concluded that chromosome 13 deletion may be involved in initiation of a subset of PCL, and the region of loss at chromosome 13 locates between 13q14 and 13q31 in PCL. FISH is a more rapid, exact and sensitive technique in analysis of chromosome 13 deletion in PCL.
Xu
Wei
W
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Li
Jian-Yong
JY
Fan
Lei
L
Chen
Li-Juan
LJ
Qiu
Hai-Rong
HR
Wang
Rong
R
Qiao
Chun
C
Lu
Hua
H
D000328:Adult; D000368:Aged; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007952:Leukemia, Plasma Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017384:Sequence Deletion