Protective effect of polygoni cuspidati radix and emodin on Vibrio vulnificus cytotoxicity and infection.
Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.
Kim
Jong Ro
JR
Department of Oriental Medicine Materials, Dongshin University, Jeonnam, 520-714, Republic of Korea.
Oh
Dool-Ri
DR
Cha
Mi Hye
MH
Pyo
Byoung Sik
BS
Rhee
Joon Haeng
JH
Choy
Hyon E
HE
Oh
Won Keun
WK
Kim
Young Ran
YR
D000818:Animals; D001431:Bacteriological Techniques; D001681:Biological Assay; D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D004642:Emodin / Q000494:pharmacology* / Q000627:therapeutic use; D045468:Fallopia japonica / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012623:Seawater / Q000382:microbiology; D014735:Vibrio Infections / Q000188:drug therapy* / Q000382:microbiology / Q000401:mortality; D041261:Vibrio vulnificus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity
D003470:Culture Media; D010936:Plant Extracts; D004642:Emodin