title: Transplanted endothelial progenitor cells increase neo-vascularisation of rat pre-fabricated flaps.
abstract: BACKGROUND
  Flap pre-fabrication is dependent on the eventual re-vascularisation of the implanted vascular carrier and the presence of a desirable, donor-skin site. However, insufficient neo-vascularisation and subsequent necrosis is an obstacle for this technique. A recent discovery demonstrated that endothelial progenitor cells (EPCs) augment post-natal neo-vascularisation in ischaemic tissues. As a result, we examined whether transplantation of bone-marrow-derived EPCs (BM-EPCs) increases neo-vascularisation and augments the survival areas of pre-fabricated flap in a rat model.
  
  
  METHODS
  Rat bone-marrow-derived mononuclear cells (BM-MNCs) were isolated by density gradient centrifugation and cultured in EGM-2MV. The EPCs derived from BM-MNCs were identified by surface makers such as CD34, KDR, CD133 and double-positive staining with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine-labelled acetylated low-density lipoprotein (Dil-Ac-LDL) and FITC-labelled Ulex europaeus agglutinin-1 (FITC-UEA-1). Pre-fabricated flaps were created by ligating the right femoral vascular pedicle and implanting it underneath the abdominal flap. Forty-five rats were randomly divided into three equal groups. The implantation site around the pedicle was injected subcutaneously with fluorescence-labelled BM-EPCs in group I (n=15), with vascular endothelium growth factor (VEGF) protein in group II (n=15) and with phosphate-buffered saline (PBS) in control group III. Four weeks after injection, the abdominal island flap was elevated and sutured back. Then, neo-vascularisation and flap viability was evaluated on day 7. The labelled EPCs were examined by fluorescence microscopy.
  
  
  RESULTS
  After 7 days of culture, the attached cells were spindle shaped and expressed CD34, KDR and CD133. These cells incorporated DiI-Ac-LDL and bound FITC-UEA-1. Greater augmentation of flap survival (87.26+/-10.13% vs. 66.13+/-9.9% and 55.59+/-13.06%, P<0.001), higher capillary density (38.67+/-9.52 capillaries per mm(2) vs. 25.83+/-6.34 capillaries per mm(2) and 26.5+/-5.61 capillaries per mm(2), P<0.05) and larger vascular territories on the microangiogram were observed in the EPCs-treated group relative to the other two groups. The labelled cells formed new vessel structures and expressed von Willebrand factor (vWF) in the pre-fabricated flap.
  
  
  CONCLUSIONS
  Local transplantation of BM-EPCs may be a useful strategy for increasing the survival of pre-fabricated flaps, which is consistent with 'therapeutic vasculogenesis'. EPCs are superior to VEGF in their neo-vascularisation ability.
authors.affiliation: Department of Plastic & Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Medical College of Jiao Tong University, 639 Zhizaoju Road, Shanghai 200011, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zan
authors.forename: QingFeng
authors.initials: Q
authors.lastname: Li
authors.forename: JiYing
authors.initials: J
authors.lastname: Dong
authors.forename: ShengWu
authors.initials: S
authors.lastname: Zheng
authors.forename: Yun
authors.initials: Y
authors.lastname: Xie
authors.forename: Dong
authors.initials: D
authors.lastname: Yu
authors.forename: DanNing
authors.initials: D
authors.lastname: Zheng
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D042783:Endothelial Cells / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D013524:Surgical Flaps / Q000098:blood supply*