Cutting edge: antigen presentation to CD8 T cells after influenza A virus infection.
Influenza A virus infection induces massive inflammation and lung damage. Activation of CD8 T cells by dendritic cells (DCs) is necessary to control disease. We undertook studies to track directly Ag presentation to CD8 T cells in vivo through the first 72 h after infection with OVA-expressing influenza A virus. We found that Ag presentation by DCs occurs strictly in the draining lymph nodes and not within the lung itself. Surprisingly, Ag presentation was found to be mediated by a CD11b(+) DC population. Finally, the expression of antigenic complexes on DCs correlated with the location and timing of CD8 T cell activation. These results have implications for approaches to control influenza A virus infection.
Ingulli
Elizabeth
E
Department of Pediatrics, University of California San Diego, La Jolla, CA 92093, USA. eingulli@ucsd.edu
Funatake
Castle
C
Jacovetty
Erica L
EL
Zanetti
Maurizio
M
D000281:Administration, Intranasal; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000821:virology; D006183:H-2 Antigens / Q000096:biosynthesis / Q000235:genetics; D016056:Immunodominant Epitopes / Q000378:metabolism; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D016056:Immunodominant Epitopes; C110366:OVA-8; D010446:Peptide Fragments; D010047:Ovalbumin