title: Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction.
abstract: In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chung-buk, Korea.
authors.forename: Ekyune
authors.initials: E
authors.lastname: Kim
authors.forename: Ki-Eun
authors.initials: KE
authors.lastname: Park
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Dong Chul
authors.initials: DC
authors.lastname: Baek
authors.forename: Jae-Woong
authors.initials: JW
authors.lastname: Lee
authors.forename: Sang-Rae
authors.initials: SR
authors.lastname: Lee
authors.forename: Myeong-Su
authors.initials: MS
authors.lastname: Kim
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Chan-Shick
authors.initials: CS
authors.lastname: Kim
authors.forename: Deog-Bon
authors.initials: DB
authors.lastname: Koo
authors.forename: Han-Seok
authors.initials: HS
authors.lastname: Kang
authors.forename: Zae-Young
authors.initials: ZY
authors.lastname: Ryoo
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D015153:Blotting, Western / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D018628:Gene Dosage; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013552:Swine / Q000235:genetics / Q000502:physiology*
chemical_list: D020033:Protein Isoforms; D004247:DNA; D051722:ADAM Proteins