Efficient replication of human immunodeficiency virus type 1 in resting CD4+ T lymphocytes is induced by coculture with autologous dendritic cells in the absence of foreign antigens.
Dendritic cells (DC) are considered to be important contributors to human immunodeficiency virus type 1 (HIV-1) transmission and pathogenesis. As the first target cells in mucosal tissues, they can be become productively infected and can also capture virions and transfer them efficiently to CD4(+) T cells located within lymphoid tissues. Resting CD4(+) T cells appear to be another major target of HIV-1 in vivo, yet several blocks restrict replication in such cells. We report here that physical contact between virus-infected quiescent CD4(+) T cells and uninfected autologous immature DC in the absence of any foreign antigen relieves these restrictions, allowing a highly productive HIV-1 replication.
Barat
Corinne
C
Centre de Recherche en Infectiologie, Québec (QC), Canada G1V 4G2.
Gilbert
Caroline
C
Tremblay
Michel J
MJ
D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D017930:Genes, Reporter; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D014779:Virus Replication
D016655:HIV Core Protein p24; D012367:RNA, Viral; D008156:Luciferases