[Role of membrane-bound human leukocyte antigen G in inducing immune tolerance after allogeneic hematopoietic stem cell transplantation].
OBJECTIVE
To evaluate the role of human leukocyte antigen G (HLA-G) in the better effect of allogenetic bone marrow transplantation than that of peripheral blood stem cell transplantation.


METHODS
Flow cytometry was used to detect the expression of membrane-bound HLA-G (mHLA-G) on donor peripheral blood (PBC) or bone marrow (BM) mononuclear cells. The levels of soluble HLA-G (sHLA-G) in the plasma and bone marrow fluid were determined using enzyme-linked immunosorbent assay (ELISA) before and after granulocyte colony-stimulating factor (G-CSF) mobilization.


RESULTS
The mean levels of mHLA-G after G-CSF mobilization in the PBC and BM were significantly higher than that before G-CSF mobilization (P=0.001 and 0.000), but the plasma levels of sHLA-G showed no significant changes after the mobilization (P=0.279). The mean levels of sHLA-G in the BM fluid significantly increased (P=0.002) to a level higher than that in the PBC after G-CSF mobilization (P=0.004).


CONCLUSIONS
HLA-G plays an important role in immune tolerance after hematopoietic stem cell transplantation with G-CSF mobilization.
DU
Xuan
X
Department of Hemotology, Nanfang hospital, Southern Medical University, Gunaghzou 510515, China.
Wu
Xiu-li
XL
Li
Rui
R
Zhang
Yu
Y
Fan
Zhi-ping
ZP
Yi
Zheng-shan
ZS
Liu
Qi-fa
QF
D000328:Adult; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006680:HLA Antigens / Q000276:immunology* / Q000378:metabolism; D059951:HLA-G Antigens; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D015395:Histocompatibility Antigens Class I / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D008297:Male; D008875:Middle Aged
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D016179:Granulocyte Colony-Stimulating Factor