CAP (Cbl associated protein) regulates receptor-mediated endocytosis.
CAP (c-Cbl associated protein)/ponsin belongs to a family of adaptor proteins implicated in cell adhesion and signaling. Here we show that CAP binds to and co-localizes with the essential endocytic factor dynamin. We demonstrate that CAP promotes the formation of dynamin-decorated tubule like structures, which are also coated with actin filaments. Accordingly, we found that the expression of CAP leads to the inhibition of dynamin-mediated endocytosis and increases EGFR stability. Thus, we suggest that CAP may coordinate the function of dynamin with the regulation of the actin cytoskeleton during endocytosis.
Tosoni
Daniela
D
IFOM, Istituto FIRC di Oncologia Molecolare, Via Adamello 16, 20139 Milan, Italy.
Cestra
Gianluca
G
D000199:Actins / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D034285:Dynamin II / Q000378:metabolism*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008840:Microfilament Proteins / Q000378:metabolism*; D051381:Rats
D000199:Actins; D008840:Microfilament Proteins; C119201:ponsin; D034285:Dynamin II