Prenatal exposures to LTP-patterned magnetic fields: quantitative effects on specific limbic structures and acquisition of contextually conditioned fear.
Weak (<1 microT) complex magnetic fields (CMFs) may exert their behavioral influences through the hippocampus by resonating by accident or design with intrinsic electrical patterns. Rats were exposed prenatally to one of four intensities of a CMF (either <5 nanoTesla [nT], 10-50 nT, 50-500 nT, or 500-1000 nT) designed to interact with the process of Long-Term Potentiation (LTP) in the hippocampus. Rats then underwent testing in the forced swim, open field, and fear-conditioning procedures. The cell densities of all amygdaloid nuclei, specific hypothalamic structures, and the major regions of the hippocampus were quantified. Results showed that acquisition of conditioned fear was strongly inhibited in animals exposed to LTP-CMFs. Rats exposed to intensities above 10 nT showed decreased cell density in the CA2 fields of the hippocampus; more neurons were present in the CA1 fields of rats exposed to the 10-50 nT intensities compared to all other groups. A decrease in cell density in the medial preoptic nucleus was linearly dependent on field intensity. In the forced-swim test, swimming was decreased in rats that had been exposed to low (10-50 nT) and medium intensity (50-500 nT) LTP-CMFs in a manner consistent with monoamine modulation. In the open field, exposed rats were indistinguishable from controls. These findings support the hypothesis that continuous exposure during prenatal development to CMFs designed to simulate intrinsic LTP within the hippocampus can affect adult behaviors specific to this structure and produce quantitative alterations in neuronal density.
Whissell
P D
PD
Neuroscience Research Group, Department of Biology, Laurentian University, Sudbury, Ontario, Canada.
Tsang
E W
EW
Mulligan
B P
BP
Persinger
M A
MA
D000818:Animals; D001362:Avoidance Learning / Q000502:physiology / Q000528:radiation effects; D002452:Cell Count; D003213:Conditioning, Psychological / Q000502:physiology / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005239:Fear / Q000502:physiology / Q000528:radiation effects; D005260:Female; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D008032:Limbic System / Q000473:pathology / Q000503:physiopathology* / Q000528:radiation effects*; D017774:Long-Term Potentiation / Q000502:physiology / Q000528:radiation effects*; D008297:Male; D055495:Neurogenesis / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000473:pathology / Q000528:radiation effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology / Q000503:physiopathology*; D011301:Preoptic Area / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar