Determination of artesunate using reversed-phase HPLC at increased temperature and ELSD detection.
Artesunate (ART) determination can be performed by evaporative light scattering detection with mobile phase composed of CH(3)CN/HCOOH 0.01 M (40:60 v/v; pH 2.85). Evaporative light scattering detection instead of UV detection allowed to improve the sensitivity and the LOD. However, the evaporative light scattering detection response of dihydro-artemisinin appears weaker than for ART, whereas with UV detection the response of ART and dihydroartemisinin seemed similar. Constant analysis time was obtained on using the mobile phase with a flow rate of 0.5 mL/min and column temperature at 60 degrees C instead of 0.7 mL/min at room temperature. This led to less solvent consumption. Moreover, decrease in the flow rate and increase in the column temperature were advantageous for higher sensitivity with both evaporative light scattering detection and UV detection. ART determination in rectal gel and suppositories were compared with these different detection modes and similar results were obtained.
Gaudin
Karen
K
PPF-Parasitologie-Pharmacie, Faculté de Pharmacie, Université Bordeaux 2, Bordeaux, France. karen.gaudin@u-bordeaux2.fr
Kauss
Tina
T
Lagueny
Anne-Marie
AM
Millet
Pascal
P
Fawaz
Fawaz
F
Dubost
Jean-Pierre
JP
D037621:Artemisinins / Q000032:analysis* / Q000737:chemistry*; D000077332:Artesunate; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D005782:Gels / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D012996:Solutions; D013696:Temperature; D014835:Volatilization
D037621:Artemisinins; D005782:Gels; D012996:Solutions; D000077332:Artesunate