title: Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0).
abstract: In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Fukuoka, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamao
authors.forename: Yuki
authors.initials: Y
authors.lastname: Eshita
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kihara
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Satho
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuroda
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sekizuka
authors.forename: Miho
authors.initials: M
authors.lastname: Nishimura
authors.forename: Kouji
authors.initials: K
authors.lastname: Sakai
authors.forename: Shumpei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
authors.forename: Yupha
authors.initials: Y
authors.lastname: Rongsriyam
authors.forename: Narumon
authors.initials: N
authors.lastname: Komalamisra
authors.forename: Raweewan
authors.initials: R
authors.lastname: Srisawat
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyata
authors.forename: Akira
authors.initials: A
authors.lastname: Sakata
authors.forename: Masato
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Manabu
authors.initials: M
authors.lastname: Nakashima
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kashige
authors.forename: Fumio
authors.initials: F
authors.lastname: Miake
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.forename: Mina
authors.initials: M
authors.lastname: Nakauchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000330:Aedes / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002043:Bunyaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000737:chemistry; D007814:Larva / Q000821:virology; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D014709:Vero Cells; D014773:Virology / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral