Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings.
The present study investigated the possible mediatory role of salicylic acid (SA) in protecting plants from cadmium (Cd) toxicity. The exposure of pea plants to increasing Cd concentrations (0.5, 1.0, 2.0 and 5.0 microM) during early stages of their establishment, caused a gradual decrease in shoot and root fresh weight accumulation, the rate of CO2 fixation and the activity of ribulose-1,5-bisphosphate carboxylase (RuBPC, E.C. 4.1.1.39), the effect being most expressed at higher Cd concentrations. In vivo the excess of Cd-induced alterations in the redox cycling of oxygen-evolving centers and the assimilatory capacity of the pea leaves as revealed by changes in thermoluminescence emission after flash illumination. The levels of some important parameters associated with oxidative stress, namely lipid peroxidation, electrolyte leakage and proline production were increased. Seed pretreatment with SA alleviated the negative effect of Cd on growth, photosynthesis, carboxylation reactions, thermoluminescence characteristics and chlorophyll content, and led to decrease in oxidative injuries caused by Cd. The data suggest that the beneficial effect of SA during an earlier growth period could be related to avoidance of cumulative damage upon exposure to cadmium thus reducing the negative consequences of oxidative stress caused by heavy metal toxicity. In addition, the observed high endogenous levels of SA after treatment with Cd suggests that SA may act directly as an antioxidant to scavenge the reactive oxygen species and/or indirectly modulate redox balance through activation of antioxidant responses. Taken together these evidences could explain at some extend the protective role of SA on photochemical activity of chloroplast membranes and photosynthetic carboxylation reactions in Cd-stressed pea plants.
Popova
Losanka P
LP
Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria. lpopova@obzor.bio21.bas.bg
Maslenkova
Liliana T
LT
Yordanova
Rusina Y
RY
Ivanova
Albena P
AP
Krantev
Aleksander P
AP
Szalai
Gabriella
G
Janda
Tibor
T
D002104:Cadmium / Q000633:toxicity*; D005227:Fatty Acids / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D049449:Luminescence; D018532:Pisum sativum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*
D005227:Fatty Acids; D002104:Cadmium; D006861:Hydrogen Peroxide; D020156:Salicylic Acid