title: Detection of Legionella pneumophila by PCR-ELISA method in industrial cooling tower water.
abstract: Water supply and Cooling Tower Water (CTW) are among the most common sources of Legionella pneumophila (LP) contamination. A nonradio active method is described to detect LP in industrial CTW samples. DNA was purified and amplified by nested -PCR with amplimers specific for the 16s rRNA gene of LP. The 5' end biotinylated oligomer probe was immobilized on sterptavidin B coated microtiter plates. The nested-PCR product was labeled with digoxigenin and then hybridized with 5'-biotinylated probes. The amplification products were detected by using proxidase-labled anti dioxygenin antibody in a colorimetric reaction. The assay detected LP present in 1 L of 5 CTW samples examined. All of the samples were Legionella positive in both culture and PCR-ELISA methods. The PCR-ELISA assay appears to exhibit high specificity and is a more rapid technique in comparison with bacterial culture method. Thus could prove suitable for use in the routine examination of industrial CTW contamination.
authors.affiliation: Department of Biotechnology, Research Institute of Petroleum Industry, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Soheili
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nejadmoghaddam
authors.forename: Mohammad
authors.initials: M
authors.lastname: Babashamsi
authors.forename: Jamileh
authors.initials: J
authors.lastname: Ghasemi
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi Tehrani
mesh_terms: D019921:Biotinylation; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000737:chemistry; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*; D014881:Water Supply
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S