title: [Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910].
abstract: OBJECTIVE
  To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.
  
  
  METHODS
  The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.
  
  
  RESULTS
  Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).
  
  
  CONCLUSIONS
  The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.
authors.affiliation: PLA Institute of Clinical Laboratory Medicine, Nanjing University School of Medicine/Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.forename: Fang-Qiu
authors.initials: FQ
authors.lastname: Li
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Han
authors.forename: Li-Kui
authors.initials: LK
authors.lastname: Wang
authors.forename: Ya-Yi
authors.initials: YY
authors.lastname: Hou
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C090172:SPA17 protein, human