Towards a semi-automatic functional annotation tool based on decision-tree techniques.
BACKGROUND
Due to the continuous improvements of high throughput technologies and experimental procedures, the number of sequenced genomes is increasing exponentially. Ultimately, the task of annotating these data relies on the expertise of biologists. The necessity for annotation to be supervised by human experts is the rate limiting step of the data analysis. To face the deluge of new genomic data, the need for automating, as much as possible, the annotation process becomes critical.


RESULTS
We consider annotation of a protein with terms of the functional hierarchy that has been used to annotate Bacillus subtilis and propose a set of rules that predict classes in terms of elements of the functional hierarchy, i.e., a class is a node or a leaf of the hierarchy tree. The rules are obtained through two decision-trees techniques: first-order decision-trees and multilabel attribute-value decision-trees, by using as training data the proteins from two lactic bacteria: Lactobacillus sakei and Lactobacillus bulgaricus. We tested the two methods, first independently, then in a combined approach, and evaluated the obtained results using hierarchical evaluation measures. Results obtained for the two approaches on both genomes are comparable and show a good precision together with a high prediction rate. Using combined approaches increases the recall and the prediction rate.


CONCLUSIONS
The combination of the two approaches is very encouraging and we will further refine these combinations in order to get rules even more useful for the annotators. This first study is a crucial step towards designing a semi-automatic functional annotation tool.
Azé
Jérôme
J
LRI - CNRS UMR 8623 - University Paris-Sud 11, F-91405 Orsay Cedex, France.
Gentils
Lucie
L
LRI - CNRS UMR 8623 - University Paris-Sud 11, F-91405 Orsay Cedex, France.
Toffano-Nioche
Claire
C
LRI - CNRS UMR 8623 - University Paris-Sud 11, F-91405 Orsay Cedex, France.
Loux
Valentin
V
INRA, Unité Mathématique, Informatique et Génome UR1077, F-78352 Jouy-en-Josas, France.
Gibrat
Jean-François
JF
INRA, Unité Mathématique, Informatique et Génome UR1077, F-78352 Jouy-en-Josas, France.
Bessières
Philippe
P
INRA, Unité Mathématique, Informatique et Génome UR1077, F-78352 Jouy-en-Josas, France.
Rouveirol
Céline
C
LIPN - UMR CNRS 7030 - Institut Galilée - University Paris-Nord, F-93430 Villetaneuse, France.
Poupon
Anne
A
IBBMC - CNRS UMR 8619 - University Paris-Sud 11, F-91405 Orsay Cedex, France.
Froidevaux
Christine
C
LRI - CNRS UMR 8623 - University Paris-Sud 11, F-91405 Orsay Cedex, France.