Assessing intraocular pressure by rebound tonometer in rats with an air-filled anterior chamber.
OBJECTIVE
To compare rebound tonometer and cannulation as methods for measuring intraocular pressure (IOP) in rats.


METHODS
The accuracy of the TonoLab rebound tonometer was determined in eight cannulated rat eyes. IOP was manipulated by changing air pressure from 20 to 100 mmHg at 10-mmHg intervals, and the IOP was measured with the rebound tonometer at each level. The average value of three repeated pressure readings was recorded. Correlation analysis and comparison with the Bland and Altman method were performed. The intraclass correlation coefficient was calculated to assess intraoperator variability.


RESULTS
The IOP values measured with the TonoLab rebound tonometer were well correlated with the actual IOP (r (2) = 0.963, P = 0.01). The mean of the difference between the rebound tonometer and actual (cannulation) IOP was 7.41 +/- 7.87%. The intraclass correlation coefficient was 0.9, indicating low intraoperator variability.


CONCLUSIONS
The rebound tonometer showed high accuracy and reliability for IOP measurement in rat eyes.
Lee
Eun Ji
EJ
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.
Park
Ki Ho
KH
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. kihopark@snu.ac.kr.|Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea. kihopark@snu.ac.kr.
Kim
Dong Myung
DM
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.|Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea.
Yoo
Young Cheol
YC
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.|Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea.|Department of Ophthalmology, Gangdong Sacred Heart Hospital Hallym University College of Medicine, Seoul, Korea.
Kang
Shin Hee
SH
Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.|The Armed Forces Yangju Hospital, Yangju, Korea.
Kim
Yu Jeong
YJ
Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea.
D000388:Air; D000818:Animals; D000867:Anterior Chamber; D002404:Catheterization; D007429:Intraocular Pressure; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014065:Tonometry, Ocular / Q000295:instrumentation* / Q000379:methods* / Q000592:standards