Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance.
We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype.
Kantawong
Fahsai
F
Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK. f.kantawong.1@research.gla.ac.uk
Burchmore
Richard
R
Wilkinson
Chris D W
CD
Oreffo
Richard O C
RO
Dalby
Matthew J
MJ
D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D018625:Microscopy, Atomic Force; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D011506:Proteins