title: Redox properties and crystal structures of a Desulfovibrio vulgaris flavodoxin mutant in the monomeric and homodimeric forms.
abstract: The mutant S64C of the short-chain flavodoxin from Desulfovibrio vulgaris has been designed to introduce an accessible and reactive group on the protein surface. Crystals have been obtained of both the monomeric and homodimeric forms of the protein, with the cofactor FMN in either the oxidized or the one electron-reduced (semiquinone) state, and the structures have been determined to high resolution. The redox properties of the different species have been investigated and the variations observed with respect to wild type have been related to the structural changes induced by the mutation and S-S bridge formation.
authors.affiliation: Imperial College London, Division of Molecular Biosciences, South Kensington, SW72AZ, London, United Kingdom.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fantuzzi
authors.forename: Roberto
authors.initials: R
authors.lastname: Artali
authors.forename: Gabriella
authors.initials: G
authors.lastname: Bombieri
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Marchini
authors.forename: Fiorella
authors.initials: F
authors.lastname: Meneghetti
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Gilardi
authors.forename: Sheila J
authors.initials: SJ
authors.lastname: Sadeghi
authors.forename: Davide
authors.initials: D
authors.lastname: Cavazzini
authors.forename: Gian Luigi
authors.initials: GL
authors.lastname: Rossi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D016969:Desulfovibrio vulgaris / Q000737:chemistry / Q000235:genetics*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D005418:Flavodoxin / Q000737:chemistry* / Q000235:genetics*; D008623:Mercaptoethanol / Q000494:pharmacology; D008958:Models, Molecular; D010084:Oxidation-Reduction
chemical_list: D005418:Flavodoxin; D008623:Mercaptoethanol