Oxidative stress and delta-ALA-D activity in different conditioning regimens in allogeneic bone marrow transplantation patients.
OBJECTIVE
To compare different conditioning regimens (CR), in order to determine whether either of them could be less toxic to allogeneic bone marrow transplantation (BMT) patients in terms of oxidative stress and also analyze delta-ALA-D activity as a possible marker of oxidative stress.


METHODS
Lipid peroxidation, vitamin C, thiol groups levels and catalase, superoxide dismutase and delta-ALA-D activity were determined in 21 healthy controls, 5 patients with fludarabine+cyclophosphamide (FluCy) CR, 12 with busulfan+cyclophosphamide (BuCy) and 4 with cyclophosphamide+total body irradiation (CyTBI).


RESULTS
There were a decrease in enzymatic and non enzymatic antioxidants, in delta-ALA-D activity, and in all CRs and an increase in lipid peroxidation more pronounced in CyTBI CR.


CONCLUSIONS
All CRs promoted oxidative stress in allogeneic BMT patients, but this was more pronounced with CyTBI and delta-ALA-D activity seemed to be an additional biomarker of oxidative stress in these patients.
Gonçalves
Thissiane L
TL
Departamento de Química, CCNE, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. thissianegoncalves@yahoo.com.br
Benvegnú
Dalila M
DM
Bonfanti
Gabriela
G
Frediani
Andressa V
AV
Pereira
Dalnei V
DV
Rocha
João B T
JB
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002374:Catalase / Q000378:metabolism; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014740:Vidarabine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology
D000975:Antioxidants; D003520:Cyclophosphamide; D002374:Catalase; D013482:Superoxide Dismutase; D000623:Porphobilinogen Synthase; D014740:Vidarabine; C024352:fludarabine; D001205:Ascorbic Acid