Glucosinolate metabolites required for an Arabidopsis innate immune response.
The perception of pathogen or microbe-associated molecular pattern molecules by plants triggers a basal defense response analogous to animal innate immunity and is defined partly by the deposition of the glucan polymer callose at the cell wall at the site of pathogen contact. Transcriptional and metabolic profiling in Arabidopsis mutants, coupled with the monitoring of pathogen-triggered callose deposition, have identified major roles in pathogen response for the plant hormone ethylene and the secondary metabolite 4-methoxy-indol-3-ylmethylglucosinolate. Two genes, PEN2 and PEN3, are also necessary for resistance to pathogens and are required for both callose deposition and glucosinolate activation, suggesting that the pathogen-triggered callose response is required for resistance to microbial pathogens. Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens.
Clay
Nicole K
NK
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA.
Adio
Adewale M
AM
Denoux
Carine
C
Jander
Georg
G
Ausubel
Frederick M
FM
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D005408:Flagellin / Q000276:immunology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005936:Glucans / Q000096:biosynthesis; D005961:Glucosinolates / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007113:Immunity, Innate; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology; D020156:Salicylic Acid / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D005030:Ethylenes; D005936:Glucans; D005961:Glucosinolates; D007211:Indoles; C523452:MYB51 protein, Arabidopsis; C510424:PDR8 protein, Arabidopsis; D010446:Peptide Fragments; D014157:Transcription Factors; D005408:Flagellin; C036025:4-methoxy-3-indolylmethyl glucosinolate; C048306:callose; C036216:ethylene; C016516:indole-3-acetonitrile; D019881:Aminoacyltransferases; C502365:AT5G44070 protein, Arabidopsis; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D009699:N-Glycosyl Hydrolases; C505663:PEN2 protein, Arabidopsis; D020156:Salicylic Acid