Versatile method for electroosmotic flow measurements in microchip electrophoresis.
A novel versatile method for the determination of low or high electroosmotic mobility values in microdevices of variable microchannel design is presented. The electroosmotic flow (EOF) calculation is based on the difference between the apparent and effective mobilities of a reference compound. The proposed method uses microchip frontal electrophoresis for the determination of these mobilities. This requires simple monochannel microchip design and demonstrates versatile and time-saving procedure when compared to conventional current monitoring method when measuring low EOF. It has been applied successfully to the characterization of different coating procedure in glass and poly(dimethylsiloxane) microchips.
Shakalisava
Yuliya
Y
Laboratoire de Physico-Chimie, Institute Curie, UMR-CNRS 168, Paris, France.
Poitevin
Martine
M
Viovy
Jean-Louis
JL
Descroix
Stephanie
S
D004129:Dimethylpolysiloxanes / Q000737:chemistry; D053841:Electroosmosis / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D015203:Reproducibility of Results
D004129:Dimethylpolysiloxanes; D019793:Fluorescein