title: [Effect of tissue factor pathway inhibitor on apoptosis of rat mesangial cells and Fas and bcl-2 expression].
abstract: OBJECTIVE
  To study the biological impact and mechanism of recombinant tissue factor pathway inhibitor (rTFPI) on apoptosis of rat kidney mesangial cells (MsC).
  
  
  METHODS
  TFPI expression in human glomerular minor lesion (GML), mesangial proliferative glomerulonephritis (MPGN) and cultured rat MsC was detected using immunohistochemistry and immunofluorescence, respectively. Rat MsC were incubated with rTFPI and its variant peptides. Morphological changes of apoptosis were investigated by Hoechst 33258 and the apoptotic rate was assessed by flow cytometry. DNA fragmentation and effect of rTFPI on expression of caspase-3, Fas and bcl-2 were studied using gel electrophoresis and Western blot respectively.
  
  
  RESULTS
  The expression of TFPI in MPGN was higher than that in GML. TFPI was expressed in cultured rat mesangial cells. Apoptosis of MsC was induced by rTFPI, especially by its C-termianl, in a dose- and time-dependent manner. Apoptosis ratios of MsC treated with rTFPI were 2.1, 3.0 and 4.9 times more than control, respectively. Expression of gene caspase-3 and Fas was up-regulated in a dose-dependent manner wherease bcl-2 expression did not show any changes.
  
  
  CONCLUSIONS
  rTFPI induces apoptosis in cultured rat mesangial cells by its C-terminal possibly via Fas/FasL pathway.
authors.affiliation: Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Lin
authors.forename: Duan
authors.initials: D
authors.lastname: Ma
authors.forename: Wang
authors.initials: W
authors.lastname: Liang
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Zhong-hua
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Nong
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism*; D002478:Cells, Cultured / Q000187:drug effects; D053938:DNA Fragmentation; D005434:Flow Cytometry; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism / Q000494:pharmacology*; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D051381:Rats; D051025:bcl-Associated Death Protein / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C494821:Bad protein, rat; D008074:Lipoproteins; D010455:Peptides; D051025:bcl-Associated Death Protein; C051928:lipoprotein-associated coagulation inhibitor; D053148:Caspase 3