Involvement of G-alpha protein GNA3 in production of cell wall-degrading enzymes by Trichoderma reesei (Hypocrea jecorina) during mycoparasitism against Pythium ultimum.
The involvement of the G-alpha protein GNA3 in the production of cell wall-degrading enzymes (CWDEs) by Trichoderma reesei during antagonism against Pythium ultimum was investigated. cAMP content was 2.8-fold higher in the T. reesei mutant gna3QL than in the parental TU-6. The gna3QL, like TU-6, inhibited the growth of P. ultimum in dual culture assays. Scanning electron microscopy showed that the gna3QL promoted more morphological alterations of P. ultimum cell wall than TU-6. In general, gna3QL produced higher activities of CWDEs than TU-6. We therefore suggest that CWDEs production during mycoparasitism by T. reesei against P. ultimum may be associated with the level of GNA3 activity.
do Nascimento Silva
Roberto
R
Departamento de Biologia Celular, Universidade de Brasília, Asa Norte, 70910-900 Brasilia, DF, Brazil.
Steindorff
Andrei Stecca
AS
Ulhoa
Cirano José
CJ
Félix
Carlos Roberto
CR
D000898:Antibiosis; D000242:Cyclic AMP / Q000032:analysis; D003593:Cytoplasm / Q000737:chemistry; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D011775:Pythium / Q000254:growth & development / Q000382:microbiology*; D014242:Trichoderma / Q000201:enzymology* / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D004798:Enzymes; D005656:Fungal Proteins; D037521:Virulence Factors; D000242:Cyclic AMP