Ultrasonic and electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto a titanium plasma-spray surface.
Several studies suggest that the surface coating of titanium could play an important role in bone tissue engineering. In the present study, we have followed a particular biomimetic strategy where ultrasonically or electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix on a titanium plasma-spray surface. In comparison with control conditions, the ultrasonic stimulation (average power, 149 mW; frequency, 1.5 MHz) and the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation, and increased surface coating with decorin, osteocalcin, osteopontin, and type I collagen together with higher incorporation of calcium and phosphorus inside the extracellular matrix. The immunofluorescence related to the preceding bone matrix proteins showed their colocalization in the cell-rich areas. The use of the two physical stimulations aimed at obtaining the coating of the rough titanium plasma-spray surface in terms of cell colonization and deposition of extracellular matrix. The superficially cultured biomaterial could be theoretically used, in clinical applications, as an implant for bone repair.
Fassina
Lorenzo
L
Dipartimento di Informatica e Sistemistica, Università di Pavia, Pavia, Italy. lorenzo.fassina@unipv.it
Saino
Enrica
E
Sbarra
Maria Sonia
MS
Visai
Livia
L
Cusella De Angelis
Maria Gabriella
MG
Mazzini
Giuliano
G
Benazzo
Francesco
F
Magenes
Giovanni
G
D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D055590:Electromagnetic Phenomena; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium / Q000378:metabolism*; D014465:Ultrasonics
D016326:Extracellular Matrix Proteins; D014025:Titanium