title: Evaluation of whole antioxidant defenses of human mononuclear cells by a new in vitro biological test: lack of correlation between erythrocyte and mononuclear cell resistance to oxidative stress.
abstract: OBJECTIVE
  This work aims to evaluate the resistance of mononuclear cells to oxidative stress using a "KRL" test, formerly utilized to evaluate the resistance of erythrocyte to free radicals.
  
  
  METHODS
  The "KRL" test evaluates the resistance to lysis of cells treated by free radicals generated under standardized conditions.
  
  
  RESULTS
  We defined new analytical parameters (level of radical production, time course, number of cells) to obtain an accurate assay determining the resistance to oxidative stress of mononuclear cells, in comparison to that of erythrocytes. This test allows the evaluation of change in the redox state of mononuclear cells (improved by an antioxidant mix or deteriorated by antimycin A-induced mitochondrial radical overproduction). Interestingly, our data show that the sensitivity of mononuclear cells to oxidative stress is not correlated with the susceptibility of erythrocytes to oxidative stress.
  
  
  CONCLUSIONS
  The quantification of the susceptibility of mononuclear cells to oxidative stress gives additional information (in addition to erythrocyte resistance) and could be helpful for patients with chronic inflammation.
authors.affiliation: Laboratoire de Biochimie, C.H.U. Rangueil, 1 av J.Poulhes, Toulouse, France. casparbauguil.s@chu-toulouse.fr
authors.forename: Sylvie
authors.initials: S
authors.lastname: Caspar-Bauguil
authors.forename: Nicolas
authors.initials: N
authors.lastname: Maestre
authors.forename: Céline
authors.initials: C
authors.lastname: Segafredo
authors.forename: Anne
authors.initials: A
authors.lastname: Galinier
authors.forename: Jésus
authors.initials: J
authors.lastname: Garcia
authors.forename: Michel
authors.initials: M
authors.lastname: Prost
authors.forename: Brigitte
authors.initials: B
authors.lastname: Périquet
authors.forename: Luc
authors.initials: L
authors.lastname: Pénicaud
authors.forename: Robert
authors.initials: R
authors.lastname: Salvayre
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D018929:Cell Culture Techniques; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013223:Statistics as Topic
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D011933:Reagent Kits, Diagnostic; D000968:Antimycin A; D014450:Electron Transport Complex III