title: A modified canine model of portal hypertension with hypersplenism.
abstract: OBJECTIVE
  The aim of this study was to develop and describe an experimental canine model of portal hypertension with hypersplenism.
  
  
  METHODS
  Twenty-five dogs were used randomly divided into three groups: group I (control group, n = 5), group II (PVS, n = 10) and group III (PVS + SVS, n = 10). Portal vein stenosis (PVS) was performed in dogs of group II; in group III dogs the model was first prepared by PVS and additional splenic vein stenosis 3 weeks later (PVS + SVS). Portal vein pressure (PVP), length of spleen and fluctuation of hematocyte counts were measured and recorded at the appointed times. Surgery permitted visual verification of portosystemic collateral circulation. Histopathological variation of the spleen and condition of the bone marrow hyperplasia were examined to confirm the development of hypersplenism.
  
  
  RESULTS
  Both group II and group III developed prehepatic portal hypertension; group III also presented satisfactory hypersplenism compared to the control group and group II, as documented at surgery and by hematologic and pathologic examination.
  
  
  CONCLUSIONS
  Based on this study, the modified model of portal hypertension (by PVS + SVS) appears appropriate when studying the relationship between hypersplenism and hemodynamics in portal hypertension. It is also likely to be useful in studying the influence of diseased spleen in the treatment of portal hypertension.
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou, Zhejiang Province, China.
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Chen
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liao
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiqiang
authors.initials: Q
authors.lastname: Zeng
authors.forename: Wangxun
authors.initials: W
authors.lastname: Jin
authors.forename: Hongqi
authors.initials: H
authors.lastname: Shi
authors.forename: Mengtao
authors.initials: M
authors.lastname: Zhou
mesh_terms: D000818:Animals; D003251:Constriction, Pathologic; D004195:Disease Models, Animal; D004285:Dogs; D006971:Hypersplenism / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D006975:Hypertension, Portal / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D008026:Ligation; D008297:Male; D011169:Portal Vein / Q000601:surgery; D013162:Splenic Vein / Q000601:surgery; D013536:Suture Techniques; D013997:Time Factors