title: Monoclonal antibodies to LipL32 protect against heterologous Leptospira spp. challenge.
abstract: A non-culture-based leptospirosis vaccine that cross-protects against infection caused by heterologous Leptospira spp. should replace the currently available products, which are qualitatively and quantitatively inadequate. With that in mind, two murine hybridomas secreting monoclonal antibodies (MAb) binding only to homogenates of pathogenic Leptospira spp., and not of the saprophytic L. biflexa, serogroup Patoc, serovar Patoc, were produced. The MAbs of both clones neutralized Leptospira-mediated human red blood cell lysis in vitro and rescued hamsters from lethal infection with heterologous Leptospira spp. The orthologous Leptospira spp. protein carrying the MAb epitope(s) was identified by two-dimensional gel electrophoresis (2DE)-based proteomics and database search. The epitopes of the MAbs were located on the major outer membrane protein LipL32 of the pathogenic Leptospira spp. The MAbs in their humanized version are potential leptospirosis immunotherapeutics. They are also suitable as detection reagents in antigen-based assays for the rapid diagnosis of leptospirosis. Recombinant LipL32 is a good candidate for a broad spectrum, non-culture-based leptospirosis vaccine.
authors.affiliation: Faculty of Allied Health Sciences, Thammasat University , Rangsit Center, Pathumthani, Thailand.
authors.forename: Santi
authors.initials: S
authors.lastname: Maneewatch
authors.forename: Yuwaporn
authors.initials: Y
authors.lastname: Sakolvaree
authors.forename: Patcharin
authors.initials: P
authors.lastname: Saengjaruk
authors.forename: Potjanee
authors.initials: P
authors.lastname: Srimanote
authors.forename: Pramuan
authors.initials: P
authors.lastname: Tapchaisri
authors.forename: Pongsri
authors.initials: P
authors.lastname: Tongtawe
authors.forename: Buppa
authors.initials: B
authors.lastname: Klaysing
authors.forename: Surasakdi
authors.initials: S
authors.lastname: Wongratanacheewin
authors.forename: Manas
authors.initials: M
authors.lastname: Chongsa-Nguan
authors.forename: Wanpen
authors.initials: W
authors.lastname: Chaicumpa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D006224:Cricetinae; D000939:Epitopes / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis; D006825:Hybridomas / Q000378:metabolism*; D007919:Leptospira / Q000378:metabolism*; D007922:Leptospirosis / Q000517:prevention & control*; D008074:Lipoproteins / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008647:Mesocricetus; D051379:Mice; D040901:Proteomics / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins