Toward prevention of cowdriosis.
Mass production of Ehrlichia ruminantium variants from different regions of sub-Saharan Africa is one of the difficulties that must be overcome in producing a heartwater vaccine. Vaccine productivity can be limited by endogenous induction of interferon (IFN), which inhibits the propagation of Ehrlichia ruminantium (ER) in cell culture. Different kinds of endothelial cells, in which ER multiply efficiently, could be grown in a scalable way in VueLife Teflon bags on Cytodex 3 microcarriers where bead-to-bead transfer of cells occurs. The digital holographic microscope designed at the Université Libre de Bruxelles allows detection of the most appropriate time to harvest intracellular microorganisms for vaccine production.
Pedregal
Andreia
A
Animal Cell Biotechnology, Université Libre de Bruxelles, Brussels, Belgium.
de Sousa
Daniel Ribeiro
DR
Nguyen
Hoang Nam
HN
das Neves
Eane Agapito
EA
Lowagie
Serge
S
Marique
Thierry
T
Kagye
Nathalie
N
Guerra
Ines Teixeira
IT
Kamba
Yvette
Y
Totte
Philippe
P
Vachiéry
Nathalie
N
Lefrançois
Thierry
T
Martinez
Dominique
D
Yourassowsky
Catherine
C
Callens
Natacha
N
Monnom
Olivier
O
Dubois
Frank
F
Wérenne
John
J
D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002417:Cattle; D016873:Ehrlichiosis / Q000517:prevention & control*; D006801:Humans; D006822:Hybrid Cells; D007372:Interferons / Q000378:metabolism*
D001428:Bacterial Vaccines; D007372:Interferons