title: Detection of Rickettsia spp. in Danish ticks (Acari: Ixodes ricinus) using real-time PCR.
abstract: We examined 192 Ixodes ricinus tick nymphs from Denmark for the presence of Rickettsia spp. We used a species-specific real-time PCR; 13.0% of the ticks were positive for rickettsial DNA. Only DNA from Rickettsia helvetica was found in the ticks.
authors.affiliation: Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. clv@ssi.dk
authors.forename: Claus Bo
authors.initials: CB
authors.lastname: Svendsen
authors.forename: Karen A
authors.initials: KA
authors.lastname: Krogfelt
authors.forename: Per Moestrup
authors.initials: PM
authors.lastname: Jensen
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003718:Denmark; D018884:Ixodes / Q000254:growth & development / Q000382:microbiology*; D009758:Nymph / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013045:Species Specificity; D014197:Trees
chemical_list: D004269:DNA, Bacterial