title: Analysis of spontaneous and streptonigrin-induced sister chromatid exchanges in peripheral lymphocytes of aircrew members of international flights.
abstract: In this work, we extend our previous studies concerning mutagen sensitivity in flight personnel from commercial airlines by analyzing the frequency of spontaneous and streptonigrin (SN)-induced sister chromatid exchanges (SCEs) in peripheral blood lymphocytes of 18 long-haul aircrew members from Argentina and of 18 control individuals. Statistical analysis revealed no significant differences between aircrew and controls in the background level of SCEs (p > 0.05), which suggests that chronic exposure to cosmic radiation and other occupational hazards does not affect SCEs frequency in peripheral lymphocytes of aircrews. The fact that almost no correlation was found between cumulative flight hours and the yield of spontaneous SCEs in aircrews adds further support to this assumption. Therefore, the background SCEs frequency cannot be use as a valid biomarker to determine the genotoxic effects of cosmic radiation or other occupational hazards exposure in aircrews. Following SN treatment, a significant increase in the mean frequency of SCEs was observed in the control group (p < 0.05) but not in the aircrew group (p > 0.05), suggesting that at the population level, aircrew are more resistant to the mutagenic effects of SN than controls. The reasons of this resistance remain to be determined. Since cosmic radiation had no effect on the background SCEs frequency and no relationship was found between cumulative flight hours and SCEs inducer effect by SN in aircrews, a direct effect of cosmic radiation on SN resistance should be discarded.
authors.affiliation: Laboratorio de Citogenética y Mutagénesis, Instituto Multidisciplinario de Biología Celular La Plata, Argentina.
authors.forename: Julieta
authors.initials: J
authors.lastname: Sánchez
authors.forename: Martha Susana
authors.initials: MS
authors.lastname: Bianchi
authors.forename: Vicente Rubén
authors.initials: VR
authors.lastname: Ciancio
authors.forename: Alejandro Daniel
authors.initials: AD
authors.lastname: Bolzán
mesh_terms: D000328:Adult; D000401:Aircraft / Q000592:standards; D001118:Argentina; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003359:Cosmic Radiation / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000528:radiation effects; D013308:Streptonigrin / Q000494:pharmacology*; D017132:Workplace / Q000592:standards
chemical_list: D013308:Streptonigrin