title: Effect of CoQ homologues on reactive oxygen generation by mitochondria.
abstract: Effect of CoQ compounds (Qs) on reactive oxygen (ROS) generation by mitochondrial complex I was studied using rat liver mitochondria and chemiluminescence probe L012. Kinetic analysis revealed that short chain Qs, such as Q2 and idebenone enhanced ROS generation by mitochondrial NADH oxidase system by a succinate-inhibitable mechanism. Lipid peroxidation in mitochondrial membranes induced by NADH and iron was inhibited by short chain Qs. The inhibitory activity was enhanced by co-oxidation of succinate as determined by chemiluminescence method and by electron spin resonance spectroscopy. These results suggested that the reduced form of short chain Qs inhibited mitochondrial ROS generation and lipid peroxidation.
authors.affiliation: Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Osaka, Japan. imada@med.osaka-cu.ac.jp
authors.forename: Isuke
authors.initials: I
authors.lastname: Imada
authors.forename: Eisuke F
authors.initials: EF
authors.lastname: Sato
authors.forename: Yukimi
authors.initials: Y
authors.lastname: Kira
authors.forename: Masayasu
authors.initials: M
authors.lastname: Inoue
mesh_terms: D000818:Animals; D008297:Male; D053858:Metabolic Networks and Pathways; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D017382:Reactive Oxygen Species; D014451:Ubiquinone; D010088:Oxidoreductases; C022691:succinate oxidase; C024989:coenzyme Q10; C036619:idebenone; C030571:ubiquinone 9