Effects of propofol on the mRNA expression of toll-like receptor 4 in BV-2 cells during mimic ischemia-reperfusion injury in vitro.
This study investigated the effects of propofol on the mRNA expression of Toll-like receptor-4 (TLR4) in BV-2 cells during mimic ischemia-reperfusion (I/R) injury in vitro. BV-2 cells, a mouse microglia line, were cultured and divided into 4 groups at random: control group (group C), ischemia/reperfusion group (group I/R), low-dose propofol (25 micromol/L) intervention group (group PF(25)) and high-dose propofol (100 micromol/L) intervention group (group PF(100)). The mRNA expression of TLR4 and NF-kappaB was measured by means of RT-PCR. TNF-alpha levels in the supernatants of BV-2 cells were detected by ELISA. The results showed that the mRNA expression of TLR4 and NF-kappaB was significantly higher in groups I/R, PF(25) and PF(100) than in group C (P<0.01). And the TNF-alpha level in the supernatants was elevated in groups I/R, PF(25) and PF(100) as compared with that in group C (P<0.01). After pre-treatment with propofol, the mRNA expressions of TLR4 and NF-kappa B and the TNF-alpha level were significantly decreased in groups PF(25) and PF(100) in comparison to those in group I/R (P<0.01). And the decrease in those indicators was more significant in group PF(100) than in group PF(25) (P<0.01). It was concluded that propofol exerted brain-protecting effects during I/R injury by suppressing the mRNA expressions of TLR4 and NF-kappaB and deceasing the TNF-alpha level.
Zhang
Qing
Q
Department of Anesthesia, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zqann_126@126.com
Sun
Peng
P
Zhang
Shihai
S
Tian
Yuan
Y
Zhang
Jinghui
J
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D051379:Mice; D017628:Microglia / Q000166:cytology; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D015742:Propofol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D016328:NF-kappa B; D018696:Neuroprotective Agents; D012333:RNA, Messenger; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D015742:Propofol