A novel dengue vaccine candidate that induces cross-neutralizing antibodies and memory immunity.
A novel dengue vaccine candidate comprised of a consensus dengue virus envelope protein domain III (cED III) was developed to fight against dengue virus infection. The amino acid sequence of this novel cED III was obtained by alignment of amino acid sequences from different isolates of the four serotypes of dengue viruses. A proof-of-concept study demonstrated that BALB/c mice immunized with the recombinant cED III developed neutralizing antibodies against all serotypes of dengue virus. Moreover, formulation of recombinant cED III with aluminum phosphate could induce long-lasting antibody responses and anamnestic neutralizing antibody responses following challenge with dengue virus at week 28 after priming. These results demonstrate the possibility of developing a single tetravalent vaccine against dengue viral infections.
Leng
Chih-Hsiang
CH
Vaccine Research and Development Center, National Health Research Institute, No. 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan.
Liu
Shih-Jen
SJ
Tsai
Jy-Ping
JP
Li
Yi-Shiuan
YS
Chen
Mei-Yu
MY
Liu
Hsueh-Hung
HH
Lien
Shu-Pei
SP
Yueh
Andrew
A
Hsiao
Kuang-Nan
KN
Lai
Li-Wei
LW
Liu
Feng-Chuan
FC
Chong
Pele
P
Chen
Hsin-Wei
HW
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016384:Consensus Sequence; D053059:Dengue Vaccines / Q000235:genetics / Q000276:immunology*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010710:Phosphates / Q000008:administration & dosage / Q000494:pharmacology; D016415:Sequence Alignment; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D000914:Antibodies, Viral; D053059:Dengue Vaccines; C051821:E-glycoprotein, Dengue virus type 2; D010710:Phosphates; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C012714:aluminum phosphate