[Research on application of modified polyethylene glycol hydrogels in the construction of tissue engineered heart valve].
OBJECTIVE
To explore the effect of the polyethylene glycol (PEG)-hydrogels to enhance the seeding-cells adhesion to the biomaterial scaffolds.


METHODS
Sixteen porcine aortic valves were decellularized with Triton X-100 and trypsin, then divided into A and B group, eight in each group. Group A: the donor goat's autologous bone marrow mesenchymal stem cells (BMSCs) Selected as the seeding-cells were encapsulated into the modified PEG-hydrogels to complete the process of the cells attaching to the acellular porcine aortic valves. Non-PEG but reservation of BMSCs was modified in Group B. After static culture for 7 d, the mono semilunar tissue engineering heart valve (TEHV) were implanted respectively into each donor goat's abdominal aortas. Gross and histology examination, ultrasonic scanning, electron microscopy observation and biomechanics detection were performed at 16 weeks after operation. The 8 native goat aortic valves from the donor goats were selected at the same time as control group (Group C).


RESULTS
There were much more improvements compared Group A to Group B (P < 0.05) in tensile strength [(12.9 +/- 1.3) MPa vs. (8.8 +/- 0.4) MPa], ratio of re-endothelial (84.6% vs. 14.8%) and mural thrombosis (0/8 vs. 8/8). The data illustrated the critical importance of BMSCs differentiation to endothelial and myofibroblast for remodeling into native tissue in microenvironment in vivo.


CONCLUSIONS
It is feasible to reconstruct TEHV efficiently by combining modified PEG-hydrogels with acellular biomaterial scaffold and autologous MSCs cells. It can improve the integration of the seeding-cells and scaffold. It can also protect the growth and differentiation of the BMSCs in the systemic circulation effectively.
Ouyang
Hui
H
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Zhang
Jin-bao
JB
Liu
Yang
Y
Li
Qiang
Q
Peng
Yang-hong
YH
Kang
Xiao-jun
XJ
Wang
Ying-mei
YM
Wei
Xu-feng
XF
Yi
Ding-hua
DH
Liu
Wei-yong
WY
D000818:Animals; D001021:Aortic Valve / Q000166:cytology; D001705:Bioprosthesis; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D006041:Goats; D006350:Heart Valve Prosthesis; D019918:Heart Valve Prosthesis Implantation; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011092:Polyethylene Glycols; D013552:Swine; D023822:Tissue Engineering
D020100:Hydrogels; D011092:Polyethylene Glycols