Structural and calorimetrical studies of the effect of different aminoglycosides on DPPC liposomes.
The effects of tobramycin, spectinomycin and streptomycin on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/water vesicle system were studied by using differential scanning calorimetry (DSC) and simultaneous small- and wide-angle X-ray scattering (SWAXS) in the 0-1 lipid/aminoglycoside molar range. The changes in enthalpy between the thermally adjacent phases are decreased, but the pace of decrease is totally different for the three investigated AGs. The alterations in the lamellar arrangement and the chain packing are rather tendentious and are extended by increased AG concentrations depending on the type of the AG. In the case of tobramycin and streptomycin, still sharp Bragg peaks of SAXS curves shift to smaller values of the scattering variable, while spectinomycin results in an entire loss of multilayer correlation representing an increased amount of unbound bilayers.
Oszlánczi
Agnes
A
Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, H-1521 Budapest, Hungary.
Bóta
Attila
A
Czabai
Gábor
G
Klumpp
Erwin
E
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000617:Aminoglycosides / Q000737:chemistry / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D005614:Freeze Fracturing; D008081:Liposomes / Q000737:chemistry*; D053838:Scattering, Small Angle; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D000617:Aminoglycosides; D008081:Liposomes; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine