[The influence of LIF (leukemia inhibitory factor) on the functional status of mouse line R1 embryonic stem cells].
The influence of cytokine LIF (Leukemia Inhibitory Factor) on the viability, and proliferation of mouse R1 line embryonic stem cells (ESC) and their distribution by cell cycle stages has been investigated. LIF (5-20 ng/ml) increased growth of colonies and maintained high proliferative and pluripotent properties of R1. LIF was also involved into the inhibition of spontaneous cell differentiation and apoptotic cell death; it also decreased the rations of S/G2+M cell cycle and doubling-time of cell population.
Lobanok
E S
ES
Mezhevikina
L M
LM
Belianovich
L M
LM
Petrova
R R
RR
Vasilevich
I B
IB
Volotkovskiĭ
I D
ID
Fesenko
E E
EE
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice
D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse