title: Genotypic characterization of CRF01_AE env genes derived from human immunodeficiency virus type 1-infected patients residing in central Thailand.
abstract: CRF01_AE is a major subtype of human immunodeficiency virus type 1 (HIV-1) circulating in Southeast Asia, including Thailand. HIV-1 env genes were amplified by polymerase chain reaction from blood samples of HIV-1-infected patients residing in Thailand in 2006, and cloned into the pNL4-3-derived reporter viral construct. Generated envelope protein (Env)-recombinant virus was examined for its infectivity, and then 35 infectious CRF01_AE Env-recombinant viruses were selected. Sequencing analysis revealed that the interclone variation of the deduced amino acid sequences was higher in CRF01_AE env genes isolated in 2006 than in those isolated in the early 1990s, suggesting that env gene variation has been increasing gradually among CRF01_AE viruses prevalent in Thailand. We also examined the characteristics of the deduced amino acid sequences of 35 CRF01_AE env genes. Our results may provide useful information to help in better understanding the genotype of env genes of CRF01_AE viruses currently circulating in Thailand.
authors.affiliation: Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Muang, Nonthaburi, Thailand.
authors.forename: Piraporn
authors.initials: P
authors.lastname: Utachee
authors.forename: Piyamat
authors.initials: P
authors.lastname: Jinnopat
authors.forename: Panasda
authors.initials: P
authors.lastname: Isarangkura-Na-Ayuthaya
authors.forename: Udayanga Chandimal
authors.initials: UC
authors.lastname: de Silva
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Uamporn
authors.initials: U
authors.lastname: Siripanyaphinyo
authors.forename: Nuanjun
authors.initials: N
authors.lastname: Wichukchinda
authors.forename: Kenzo
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Teruo
authors.initials: T
authors.lastname: Yasunaga
authors.forename: Pathom
authors.initials: P
authors.lastname: Sawanpanyalert
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
authors.forename: Wattana
authors.initials: W
authors.lastname: Auwanit
authors.forename: Masanori
authors.initials: M
authors.lastname: Kameoka
mesh_terms: D000595:Amino Acid Sequence; D001769:Blood / Q000821:virology; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013785:Thailand; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus