[Inhibitory effect of trehalose on phosphatidylserine exposure, osmotic fragility and membrane lipid peroxidation damage of erythrocytes induced by high concentration of glucose].
Though high concentration of glucose can benefit the survival of lyophilized human red blood cells, the high concentration of glucose can result in serious damage of red blood cells. This study was aimed to investigate the inhibitory effect of trehalose on damage of red blood cells induced by high concentration of glucose. After incubation with the high concentration of glucose buffer containing different concentrations of trehalose for three hours at 37 degrees C, the phosphatidylserine exposure and the osmotic fragility of cells were analyzed by flow cytometry and the lipid peroxidation of membrane was evaluated by TBA method. The results showed that the high concentration of glucose could lead to phosphatidylserine exposure, osmotic fragility increase, and lipid peroxidation damage which were dependent on the glucose concentrations and incubation temperature. However, trehalose could effectively prevent the phosphatidylserine exposure, osmotic fragility increase, and lipid peroxidation damage induced by high concentration glucose. With increase of the trehalose concentrations. As the trehalose concentration increases, the phosphatidylserine exposure, maloni-aldehyde concentration and cell debris rate decreased gradually. In conclusion, the high concentration of glucose can lead to phosphatidylserine exposure, osmotic fragility increase, and lipid peroxidation damage of red blood cells. However, trehalose can inhibit the damaging effects of high concentration of glucose on red blood cells, which may be useful for the application of sugars to lyophilization of red blood cells.
Quan
Guo-Bo
GB
Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China. waltq20020109@yahoo.com.cn
Han
Ying
Y
Yang
Chao
C
Hu
Wen-Bo
WB
Liu
Min-Xia
MX
Liu
An
A
Wang
Yan
Y
Wang
Jie-Xi
JX
D001793:Blood Preservation / Q000379:methods; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D005434:Flow Cytometry; D005947:Glucose / Q000009:adverse effects*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008563:Membrane Lipids / Q000378:metabolism; D009996:Osmotic Fragility / Q000187:drug effects*; D010718:Phosphatidylserines / Q000494:pharmacology; D014199:Trehalose / Q000494:pharmacology*
D008563:Membrane Lipids; D010718:Phosphatidylserines; D014199:Trehalose; D005947:Glucose