Rotenone damages striatal organotypic slice culture.
Epidemiological studies suggest the involvement of pesticides in the etiology of Parkinson's disease. Exposure to rotenone results in degeneration of the nigrostriatal pathway through inhibition of complex I. Organotypic striatal slice cultures were prepared from brains of adult mice and treated with rotenone (0.01, 0.05, 0.1, and 1 mM) for 48 h. Lactate dehydrogenase activity was elevated by 167% at 1 mM of rotenone. Using fluorescent indicators, membrane damage was up to 130% as measured by propidium iodide fluorescence, and superoxide (DHE) and nitric oxide (DAF-FM) formation were increased by 195% and 774% at 1 mM of rotenone, respectively, compared to controls. The study concludes that formation of radicals mediated striatal degeneration by rotenone.
Moldzio
Rudolf
R
Institute of Medical Chemistry, Veterinary Medical University, Vienna, Austria.
Piskernik
Christina
C
Radad
Khaled
K
Rausch
Wolf-Dieter
WD
D000818:Animals; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D007202:Indicators and Reagents / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010300:Parkinson Disease / Q000473:pathology; D011419:Propidium / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D014475:Uncoupling Agents / Q000494:pharmacology*
D007202:Indicators and Reagents; D007306:Insecticides; D017382:Reactive Oxygen Species; D014475:Uncoupling Agents; D012402:Rotenone; D009569:Nitric Oxide; D011419:Propidium; D007770:L-Lactate Dehydrogenase