Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury.
Germ cell specific apoptosis after ischemia-reperfusion (I/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment.The purpose of this study was to inhibit neutrophil recruitment in I/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion).Ten minutes after the onset of reperfusion, mice received either 100 microg of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFalpha (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 +/- 4 vs. 52 +/- 10% Gr-1 +CD11 b+ of total leucocytes; P < 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis.
Celebi
M
M
Cardiovascular Division, University of Virginia School of Medicine, Charlottesville 22908, USA. m.celebi@omu.edu.tr
Paul
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D005434:Flow Cytometry / Q000662:veterinary; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000473:pathology*; D019007:P-Selectin / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D013737:Testis / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D019007:P-Selectin