title: New approach on the development of a mucosal vaccine against strangles: Systemic and mucosal immune responses in a mouse model.
abstract: Streptococcus equi subspecies equi affects animals of Equidae family and is the causative agent of strangles, an acute, extremely contagious and deadly disease. Prolonged periods of protection associated to absence of serious adverse reactions were not yet achieved. Thus, this experimental work is focused on the study of mucosal, humoral and cellular immune responses developed in a mouse model, after the intranasal administration of S. equi antigens associated by adsorption or encapsulation to poly(lactic acid) nanospheres, modified by mucoadhesive polymers and absorption enhancers. Particles fitted the nanometer range and proteins integrity and antigenicity were not affected. PLA nanospheres induced a mixed Th1 and Th2 response, being therefore potential carriers for the delivery of S. equi antigens.
authors.affiliation: iMED, Faculdade de Farmácia da Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: H F
authors.initials: HF
authors.lastname: Florindo
authors.forename: S
authors.initials: S
authors.lastname: Pandit
authors.forename: L M D
authors.initials: LM
authors.lastname: Gonçalves
authors.forename: H O
authors.initials: HO
authors.lastname: Alpar
authors.forename: A J
authors.initials: AJ
authors.lastname: Almeida
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D018397:Equidae; D005260:Female; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D019344:Lactic Acid / Q000008:administration & dosage / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D018502:Streptococcus equi / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D016207:Cytokines; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)