The Results of Mancini's test depend on the presence bound metal cations in the test protein.
Samples of human serum gamma-globulin with specifically bound copper or zinc cations were studied in Mancini's radial immunodiffusion test with human antibodies to IgG (H+L). The intensity of antibody binding to zinc-modified protein was 10-20% higher in comparison with the reference sample, while detection of gamma-globulin with bound copper by antibodies was 20-30% lower than in the corresponding reference sample. Comparison with the results of native gamma-globulin testing indicates limitations of Mancini's method as the quantitative assay for practical diagnosis, because under certain clinical conditions the traditional method can give over- and underestimated results.
Cheknyov
S B
SB
Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. cheknev@riem.ru
Denisova
E A
EA
Mongush
E M
EM
Shukhovtseva
Yu V
YV
D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002412:Cations / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D009994:Osmolar Concentration; D011485:Protein Binding; D012698:Serologic Tests / Q000379:methods / Q000592:standards; D015032:Zinc / Q000737:chemistry / Q000378:metabolism; D005719:gamma-Globulins / Q000032:analysis* / Q000378:metabolism*
D002412:Cations; D008670:Metals; D005719:gamma-Globulins; D003300:Copper; D015032:Zinc