title: Purification and characterization of the cold-active alkaline protease from marine cold-adaptive Penicillium chrysogenum FS010.
abstract: An extracellular cold-active alkaline serine protease from Penicillium chrysogenum FS010 has been purified. The purification procedure involved: ammonium sulfate precipitation, DEAE ion-exchange chromatography and sephadex G-100 gel chromatography. SDS-PAGE of the purified enzyme indicated a molecular weight of 41,000 +/- 1,000 Da. The protease is stable in a pH range of 7.0-9.0 and has a maximum activity at pH 9.0. Compared with other industrial proteases, the enzyme shows a high hydrolytic activities at lower temperatures and a high sensitivity at a temperature over 50 degrees C. The isoelectric point of the enzyme is approximate to 6.0. Enzymatic activity is enhanced by the addition of divalent cations such as Mg(2+) and Ca(2+) and inhibited by addition of Cu(2+)and Co(2+). PMSF and DFP are its specific inhibitors. The application of the cold-active alkaline protease is extremely extensive, and widely used in detergents, feed, food, leather and many other industries.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tian
authors.forename: Yun-Hua
authors.initials: YH
authors.lastname: Hou
authors.forename: Tian-Hong
authors.initials: TH
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D010408:Penicillium chrysogenum / Q000737:chemistry / Q000201:enzymology*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D008670:Metals; D010450:Endopeptidases; C087822:alkaline protease