title: In vivo hemostatic effect of the medicinal plant extract Ankaferd  Blood Stopper in rats pretreated with warfarin.
abstract: OBJECTIVE
  Ankaferd comprises a mixture of Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum and Urtica dioica. Ankaferd Blood Stopper (ABS) has been approved in the management of bleedings. This study aimed to evaluate in vivo hemostatic effect of ABS in rats pretreated with warfarin.
  
  
  METHODS
  Wistar rats (210-270 g) were treated either with warfarin (2 mg/kg) or vehicle (0.9% NaCl) orally before bilateral hind leg amputation. ABS was administered topically to one of the amputed legs. The duration of bleeding and the amount of bleeding were measured to evaluate the hemostatic effect of ABS.
  
  
  RESULTS
  Topical ABS administration to amputed leg shortened the duration of bleeding markedly in both untreated and warfarin-treated rats by 31.9% [1.42 min (95% CI: 0.35-2.49)] and 43.5% [5.12 min (95% CI: 2.16-8.07)] respectively. The amount of bleeding in ABS-administered amputed leg showed a decrease by 53.8% in warfarin-treated group.
  
  
  CONCLUSIONS
  ABS has in vivo hemostatic actions that may provide a therapeutic potential for the management of patients with deficient primary hemostasis in clinical medicine.
authors.affiliation: Department of Hematology, Faculty of Medicine, Faith University, Ankara/Turkey.
authors.forename: Handan S
authors.initials: HS
authors.lastname: Cipil
authors.forename: Ali
authors.initials: A
authors.lastname: Kosar
authors.forename: Arif
authors.initials: A
authors.lastname: Kaya
authors.forename: Burak
authors.initials: B
authors.lastname: Uz
authors.forename: Ibrahim C
authors.initials: IC
authors.lastname: Haznedaroglu
authors.forename: Hakan
authors.initials: H
authors.lastname: Goker
authors.forename: Oktay
authors.initials: O
authors.lastname: Ozdemir
authors.forename: Mustafa
authors.initials: M
authors.lastname: Koroglu
authors.forename: Serafettin
authors.initials: S
authors.lastname: Kirazli
authors.forename: Huseyin Cahit
authors.initials: HC
authors.lastname: Firat
mesh_terms: D000287:Administration, Topical; D000671:Amputation, Surgical; D000818:Animals; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D006470:Hemorrhage / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D006487:Hemostasis / Q000187:drug effects*; D006490:Hemostatics / Q000008:administration & dosage / Q000494:pharmacology*; D006614:Hindlimb / Q000601:surgery; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014859:Warfarin
chemical_list: D006490:Hemostatics; D010936:Plant Extracts; C539751:ankaferd blood stopper; D014859:Warfarin