Elevation of efficacy of cancer vaccine combined with interferon and inducer of endogeneous interferon synthesis amixin.
OBJECTIVE
To study in vivo efficacy of combined administration of cancer vaccine (CV), interferon (IFN) and inducer of endogenous IFN - amixin.


METHODS
Sarcoma-37 cells were transplanted to female Balb/c mice. For the treatment, CV prepared from sarcoma-37 cells with the use of cytotoxic lectines from B. subtilis B-7025, murine IFN and amixin or their combinations were used. IFN production, content of circulating immune complexes and level of specific IgG antibodies in blood serum were determined by standard immunologic methods.


RESULTS
Using solid form of sarcoma-37 it has been shown that introduction of IFN and amixin significantly elevated efficacy of vaccine therapy, in particular index of tumor growth inhibition reach 89.2% and 81.7%. Upon combined use of CV and IFN or CV and amixin (25 mg/kg) respectively. Significant prolongation of average life span of the animals treated with CV and IFN or CV and amixin (25 mg/kg) has been registered (up to 92.7 -/+ 10.4 and 95.0 -/+ 6.2 days respectively, vs 46.8 -/+ 1.5 days for control animals).


CONCLUSIONS
Obtained results have shown expediency of the development of schemes for combined introduction of CV with exogenous IFN, and with inducer of endogenous IFN (amixin) for elevation of efficacy of vaccine therapy.
Potebnya
G P
GP
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv 03022, Ukraine. iris@onconet.kiev.ua
Lisovenko
G S
GS
Trokhimenko
N V
NV
Cheremshenko
N L
NL
Didenko
G V
GV
Reder
A S
AS
Andronati
S A
SA
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D005260:Female; D007167:Immunotherapy / Q000379:methods*; D007369:Interferon Inducers / Q000008:administration & dosage* / Q000276:immunology; D007372:Interferons / Q000008:administration & dosage* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012509:Sarcoma / Q000188:drug therapy*; D013994:Tilorone / Q000008:administration & dosage* / Q000276:immunology
D019496:Cancer Vaccines; D007369:Interferon Inducers; D007372:Interferons; D013994:Tilorone