title: In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus.
abstract: The purpose of the present in vitro study was to compare the cytotoxic effect of two commercially available brands of mineral trioxide cement (ProRoot MTA and MTA Angelus), modified zinc oxide-eugenol cement (SuperEBA) and resin-modified glass ionomer cement (Vitrebond) using rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC-5). The cells were cultured in typical culture conditions and exposed to the tested materials by adaptation of insert wells. The cytotoxic effect was recorded at two observation periods (24 and 72 h) by using a colorimetric assay of tetrazolium reduction (XTT method) in reference to controls. Overall, the degree of cytotoxic effect in ascending order was ProRoot MTA - MTA Angelus < SuperEBA < Vitrebond. Both MTA materials tested exerted mild suppression of cellular mitochondrial activity and may be characterized as biologically inert materials.
authors.affiliation: Department of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece. koulaouz@dent.auth.gr
authors.forename: Elisabeth A
authors.initials: EA
authors.lastname: Koulaouzidou
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Economides
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Beltes
authors.forename: George
authors.initials: G
authors.lastname: Geromichalos
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Papazisis
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity*; D002452:Cell Count; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000633:toxicity; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000633:toxicity; D006801:Humans; D007202:Indicators and Reagents; D008168:Lung / Q000166:cytology; D008422:Materials Testing; D008928:Mitochondria / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D051381:Rats; D019279:Resin Cements / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013778:Tetrazolium Salts; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D007202:Indicators and Reagents; D010087:Oxides; D019279:Resin Cements; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; C067790:Vitrabond; C084074:Super EBA