title: Synergistic effects of mutations and nanoparticle templating in the self-assembly of cowpea chlorotic mottle virus capsids.
abstract: A study of the in vitro nanoparticle-templated assembly of a mutant of cowpea chlorotic mottle virus lacking most of the N-terminal domain (residues 4-37), NDelta34, is presented. Mutant empty proteins assemble into empty capsids with a much broader distribution of sizes than the wild-type virus. This increased flexibility in the assembly outcomes is known to be detrimental for the assembly process in the presence of molecular polyanions. However, when rigid polyanionic cores are used, such as nanoparticles, the assembly process is restored and virus-like particles form. Moreover, the breadth of the nanoparticle-templated capsid size distribution becomes comparable with the wild-type virus size distribution.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Stella E
authors.initials: SE
authors.lastname: Aniagyei
authors.forename: Chelsea J
authors.initials: CJ
authors.lastname: Kennedy
authors.forename: Barry
authors.initials: B
authors.lastname: Stein
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Willits
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Young
authors.forename: Mrinmoy
authors.initials: M
authors.lastname: De
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.forename: D
authors.initials: D
authors.lastname: Srisathiyanarayanan
authors.forename: C Cheng
authors.initials: CC
authors.lastname: Kao
authors.forename: Bogdan
authors.initials: B
authors.lastname: Dragnea
mesh_terms: D017795:Bromovirus / Q000737:chemistry* / Q000648:ultrastructure*; D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure*; D003460:Crystallization / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D009154:Mutation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances