title: Angiogenesis-related gene expression profile with independent prognostic value in advanced ovarian carcinoma.
abstract: BACKGROUND
  Ovarian carcinoma is the most important cause of gynecological cancer-related mortality in Western societies. Despite the improved median overall survival in patients receiving chemotherapy regimens such as paclitaxel and carboplatin combination, relapse still occurs in most advanced diseased patients. Increased angiogenesis is associated with rapid recurrence and decreased survival in ovarian cancer. This study was planned to identify an angiogenesis-related gene expression profile with prognostic value in advanced ovarian carcinoma patients.
  
  
  RESULTS
  RNAs were collected from formalin-fixed paraffin-embedded samples of 61 patients with III/IV FIGO stage ovarian cancer who underwent surgical cytoreduction and received a carboplatin plus paclitaxel regimen. Expression levels of 82 angiogenesis related genes were measured by quantitative real-time polymerase chain reaction using TaqMan low-density arrays. A 34-gene-profile which was able to predict the overall survival of ovarian carcinoma patients was identified. After a leave-one-out cross validation, the profile distinguished two groups of patients with different outcomes. Median overall survival and progression-free survival for the high risk group was 28.3 and 15.0 months, respectively, and was not reached by patients in the low risk group at the end of follow-up. Moreover, the profile maintained an independent prognostic value in the multivariate analysis. The hazard ratio for death was 2.3 (95% CI, 1.5 to 3.2; p<0.001).
  
  
  CONCLUSIONS
  It is possible to generate a prognostic model for advanced ovarian carcinoma based on angiogenesis-related genes using formalin-fixed paraffin-embedded samples. The present results are consistent with the increasing weight of angiogenesis genes in the prognosis of ovarian carcinoma.
authors.affiliation: Department of Pathology, Hospital Universitario La Paz, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Mendiola
authors.forename: Jorge
authors.initials: J
authors.lastname: Barriuso
authors.forename: Andrés
authors.initials: A
authors.lastname: Redondo
authors.forename: Adrián
authors.initials: A
authors.lastname: Mariño-Enríquez
authors.forename: Rosario
authors.initials: R
authors.lastname: Madero
authors.forename: Enrique
authors.initials: E
authors.lastname: Espinosa
authors.forename: Juan Angel Fresno
authors.initials: JA
authors.lastname: Vara
authors.forename: Iker
authors.initials: I
authors.lastname: Sánchez-Navarro
authors.forename: Ginés
authors.initials: G
authors.lastname: Hernández-Cortes
authors.forename: Pilar
authors.initials: P
authors.lastname: Zamora
authors.forename: Elia
authors.initials: E
authors.lastname: Pérez-Fernández
authors.forename: María
authors.initials: M
authors.lastname: Miguel-Martín
authors.forename: Asunción
authors.initials: A
authors.lastname: Suárez
authors.forename: José
authors.initials: J
authors.lastname: Palacios
authors.forename: Manuel
authors.initials: M
authors.lastname: González-Barón
authors.forename: David
authors.initials: D
authors.lastname: Hardisson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D015999:Multivariate Analysis; D009389:Neovascularization, Pathologic; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D011379:Prognosis; D012313:RNA / Q000378:metabolism
chemical_list: D012313:RNA