title: Proteomic analysis of cerebrospinal fluid from patients with idiopathic temporal lobe epilepsy.
abstract: Proteomic analysis of cerebrospinal fluid (CSF) from patients with temporal lobe epilepsy (TLE) and controls was carried out using two-dimensional gel electrophoresis followed by liquid chromatography electrospray ionization tandem mass spectrometry. Five protein spots showed significant differential expression (p<0.05): vitamin D-binding protein (DBP) was elevated in the CSF of TLE patients whereas cathepsin D, apolipoprotein J, Fam3c, and superoxide dismutase 1 (SOD1) were decreased in the CSF of TLE patients. Additional six protein spots presented only in the CSF of epilepsy patients were identified as tetranectin (TN), talin-2, apolipoprotein E, immunoglobulin lambda light chain (IGL@), immunoglobulin kappa variable light chain 1-5 (IGKV1-5), and procollagen C-endopeptidase enhancer 1 (PCOLCE). Expression of DBP, SOD1 and talin-2 was validated by western blot. Our results may provide better understanding of the pathophysiologic mechanisms underlying epileptogenesis and possible epilepsy biomarkers.
authors.affiliation: Neurology Department, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1st You Yi Road, Chongqing 400016, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Guan
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Xi
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D002402:Cathepsin D / Q000134:cerebrospinal fluid; D002555:Cerebrospinal Fluid / Q000737:chemistry; D002853:Chromatography, Liquid; D051152:Clusterin / Q000134:cerebrospinal fluid; D016207:Cytokines / Q000134:cerebrospinal fluid; D015180:Electrophoresis, Gel, Two-Dimensional; D004833:Epilepsy, Temporal Lobe / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000134:cerebrospinal fluid; D040901:Proteomics / Q000379:methods; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid*; D000072105:Superoxide Dismutase-1; D016608:Talin / Q000134:cerebrospinal fluid*; D014809:Vitamin D-Binding Protein / Q000134:cerebrospinal fluid*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D051152:Clusterin; D016207:Cytokines; C514209:FAM3C protein, human; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; C000606290:SOD1 protein, human; C436513:TLN2 protein, human; D016608:Talin; D014809:Vitamin D-Binding Protein; C048648:tetranectin; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D002402:Cathepsin D