PEGylated and MMP-2 specifically dePEGylated quantum dots: comparative evaluation of cellular uptake.
Polyethylene glycol (PEG)-immobilized quantum dot (QD) nanoparticles, which could be specifically dePEGylated in response to the presence of the matrix metalloprotease-2 (MMP-2) enzyme, were prepared. The degree of PEGylation (MW 3400) on the surface of 12 nm streptavidin-coated QDs was stoichiometrically controlled by varying the feed amount of a biotin-substrate-PEG conjugate, where the substrate contained an MMP-2 cleavable peptide sequence. A biotin-cell penetrating peptide (CPP) conjugate was also immobilized onto the surface of the PEGylated QD surface to enhance the cellular uptake after dePEGylation. It was found that more than nine PEG chains per single QD were required to effectively inhibit the cellular uptake of modified QD particles down to around 20%, as compared with that of QD without PEG chains. However, the treatment of MMP-2 enzyme in the medium resulted in a substantial enhancement in the extent of QD cellular uptake by dePEGylation with concomitant resurfacing of sterically hidden CPP moieties. This study analyzed the effects of surface PEGylation density and MMP-2 specific dePEGylation on the cellular uptake of CPP-QD nanoparticles in a quantitative manner.
Mok
Hyejung
H
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea 305-701.
Bae
Ki Hyun
KH
Ahn
Cheol-Hee
CH
Park
Tae Gwan
TG
D001360:Avidin / Q000737:chemistry; D045744:Cell Line, Tumor; D005782:Gels / Q000737:chemistry; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D045663:Quantum Dots; D013379:Substrate Specificity
D005782:Gels; D001360:Avidin; D011092:Polyethylene Glycols; D020778:Matrix Metalloproteinase 2