Bioactivity-guided fractionation and GC/MS fingerprinting of Angelica sinensis and Angelica archangelica root components for antifungal and mosquito deterrent activity.
Bioassay-guided fractionation of the chloroform extract from the roots of Angelica sinensis led to isolation and characterization of (Z)-ligustilide using direct-bioautography with Colletotrichum species. The structure of (Z)-ligustilide was confirmed by (1)H and (13)C NMR spectroscopy and GC/MS. (Z)-Ligustilide deterred the biting of two mosquito species more effectively than DEET. Three different A. sinensis accessions and one Angelica archangelica root oil were evauated by GC and GC/MS, and the dominant component in A. sinensis was 61-69% (Z)-ligustilide. Two other prominent compounds in A. sinensis oils were 5.7-9.8% (E)-3-butylidene phthalide and 1.5-2.3% (Z)-3-butylidene phthalide. The main constituents that comprised A. archangelica oil were monoterpene hydrocarbons such as 24.5% alpha-pinene, 13.8% delta-3-carene, 10.1% beta-phellandrene, 8.8% p-cymene, 8.4% limonene, and 6.3% sabinene. Phthalides and monoterpene hydrocarbons were determined to be good systematic markers or chemical fingerprints for A. sinensis and A. archangelica root oils. Chemical fingerprinting by GC/MS of A. sinensis also confirmed the misidentification of one A. archangelica sample sold in the Chinese market.
Wedge
David E
DE
USDA-ARS-NPURU, University of Mississippi, University, 38677, USA. dwedge@olemiss.edu
Klun
Jerome A
JA
Tabanca
Nurhayat
N
Demirci
Betul
B
Ozek
Temel
T
Baser
Kemal Husnu Can
KH
Liu
Zhijun
Z
Zhang
Sui
S
Cantrell
Charles L
CL
Zhang
Jian
J
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005591:Chemical Fractionation; D020231:Colletotrichum / Q000187:drug effects; D009033:Culicidae / Q000187:drug effects* / Q000502:physiology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005247:Feeding Behavior; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007299:Insect Bites and Stings / Q000188:drug therapy*; D007302:Insect Repellents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D018517:Plant Roots / Q000737:chemistry*
D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D007302:Insect Repellents; C027820:ligustilide; D015107:4-Butyrolactone