title: Early postnatal ethanol exposure induces fluctuation in the expression of BDNF mRNA in the developing rat hippocampus.
abstract: Effects of early postnatal ethanol exposure on brain-derived neurotrophic factor (BDNF) mRNA expression in the rat hippocampus were investigated. Wistar rats were assigned to either ethanol treatment (ET) separation control (SC) or mother-reared control (MRC) groups. Ethanol exposure was achieved by a vapor inhalation method for 3 hours a day between postnatal days (PND) 1015. On PND 16, 20, 30, and 60, the expression of BDNF mRNA in the hippocampus was determined using real-time RT-PCR analysis. There was a significant age-related increase in the BDNF mRNA expression between PND 3060 in MRC animals. The BDNF mRNA expression in ET rats was increased at both PND 16 and 20 and thereafter decreased at PND 60 compared to SC animals. Such age-related fluctuation in the expression of BDNF mRNA differed from that of MRC animals. The exact functional implications, if any, of these ethanol-induced changes in BDNF mRNA expression remain unknown although it can be speculated that they may have an effect on the behaviors known to be influenced by the hippocampal formation.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Kagawa University, Kagawa, Japan. mikit@med.kagawa-u.ac.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kuma
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sumitani
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Kusaka
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Warita
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Naohisa
authors.initials: N
authors.lastname: Hosomi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.forename: Kuldip
authors.initials: K
authors.lastname: S Bedi
authors.forename: Susumu
authors.initials: S
authors.lastname: Itoh
authors.forename: Yu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002492:Central Nervous System Depressants / Q000097:blood / Q000633:toxicity*; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002492:Central Nervous System Depressants; D012333:RNA, Messenger; D000431:Ethanol