title: Influence of two-stage weaning with subsequent transport on body weight, plasma lipid peroxidation, plasma selenium, and on leukocyte glutathione peroxidase and glutathione reductase activity in beef calves.
abstract: An experiment was conducted to assess the effects of two-stage weaning and subsequent transport on oxidative stress markers in calves. Thirty-six crossbred Angus steers (243+/-20.8 kg; 221+/-19.7 days) were allotted to control, fenceline (FL), and nose-clip (NC) weaning groups. Steers were weighed and blood samples were collected on days -7, 0 (pre-transit) and 7 surrounding transport. Leukocytes were analyzed for glutathione peroxidase and glutathione reductase activity, whereas malondialdehyde and Se were measured in plasma. Data were analyzed using PROC MIXED with the Tukey-Kramer adjustment applied post hoc. No treatment effects were detected for blood variables or weights. Glutathione peroxidase activity was not altered by time (P = 0.27). Glutathione reductase activity, Se, and malondialdehyde declined (P<0.001) from day -7 to 7 in all steers. Treatmentxdate interaction was detected for malondialdehyde (P<0.02). Control and FL steers gained weight (P<0.03), whereas NC steers did not gain weight between days -7 and 0 (P = 0.81). All weaning treatment groups gained weight between days 0 and 7 (P<0.05). Results suggest that two-stage weaning has little effect on examined markers of oxidative stress.
authors.affiliation: Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442, USA.
authors.forename: Nathaniel C
authors.initials: NC
authors.lastname: Burke
authors.forename: Guillermo
authors.initials: G
authors.lastname: Scaglia
authors.forename: Holly T
authors.initials: HT
authors.lastname: Boland
authors.forename: William S
authors.initials: WS
authors.lastname: Swecker
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D002417:Cattle; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D007962:Leukocytes / Q000201:enzymology*; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D012643:Selenium / Q000097:blood*; D013312:Stress, Physiological; D014186:Transportation; D014886:Weaning
chemical_list: D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D012643:Selenium