title: Identification of human enteric pathogens in gull feces at Southwestern Lake Michigan bathing beaches.
abstract: Ring-billed (Larus delawarensis Ord, 1815) and herring (Larus argentatus Pontoppidan, 1763) gulls are predominant species of shorebirds in coastal areas. Gulls contribute to the fecal indicator burden in beach sands, which, once transported to bathing waters, may result in water quality failures. The importance of these contamination sources must not be overlooked when considering the impact of poor bathing water quality on human health. This study examined the occurrence of human enteric pathogens in gull populations at Racine, Wisconsin. For 12 weeks in 2004 and 2005, and 7 weeks in 2006, 724 gull fecal samples were examined for pathogen occurrence on traditional selective media (BBL CHROMagar-Salmonella, Remel Campy-BAP, 7% horse blood agar) or through the use of novel isolation techniques (Campylobacter, EC FP5-funded CAMPYCHECK Project), and confirmed using polymerase chain reaction (PCR) for pathogens commonly harbored in gulls. An additional 226 gull fecal samples, collected in the same 12-week period in 2004, from a beach in Milwaukee, Wisconsin, were evaluated with standard microbiological methods and PCR. Five isolates of Salmonella (0.7%), 162 (22.7%) isolates of Campylobacter, 3 isolates of Aeromonas hydrophila group 2 (0.4%), and 28 isolates of Plesiomonas shigelloides (3.9%) were noted from the Racine beach. No occurrences of Salmonella and 3 isolates of Campylobacter (0.4%) were found at the Milwaukee beach. A subset of the 2004 samples was also examined for Giardia and Cryptosporidium and was found to be negative. Information as to the occurrence of human pathogens in beach ecosystems is essential to design further studies assessing human health risk and to determine the parameters influencing the fate and transport of pathogens in the nearshore environment.
authors.affiliation: City of Racine, Health Department Laboratory, Room 8, 730 Washington Avenue, Racine, WI 53403, USA. julie.kinzelman@cityofracine.org
authors.forename: Julie
authors.initials: J
authors.lastname: Kinzelman
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McLellan
authors.forename: Ashley
authors.initials: A
authors.lastname: Amick
authors.forename: Justine
authors.initials: J
authors.lastname: Preedit
authors.forename: Caitlin O
authors.initials: CO
authors.lastname: Scopel
authors.forename: Ola
authors.initials: O
authors.lastname: Olapade
authors.forename: Steve
authors.initials: S
authors.lastname: Gradus
authors.forename: Ajaib
authors.initials: A
authors.lastname: Singh
authors.forename: Gerald
authors.initials: G
authors.lastname: Sedmak
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification; D016980:Aeromonas hydrophila / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001431:Bacteriological Techniques; D001493:Bathing Beaches; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification; D046091:Charadriiformes / Q000382:microbiology* / Q000469:parasitology / Q000821:virology; D003458:Cryptosporidium / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology* / Q000469:parasitology / Q000821:virology; D005872:Giardia / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008824:Michigan; D016981:Plesiomonas / Q000235:genetics / Q000302:isolation & purification; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral