title: In vitro biocompatibility of hydroxyapatite-reinforced polymeric composites manufactured by selective laser sintering.
abstract: The selective laser sintering (SLS) technique was used to manufacture hydroxyapatite-reinforced polyethylene and polyamide composites as potential customized maxillofacial implants. In vitro tests were carried out to assess cellular responses, in terms of cell attachment, morphology, proliferation, differentiation, and mineralized nodule formation, using primary human osteoblast cells. This study showed that the SLS composite processed was biocompatible, with no adverse effects observed on cell viability and metabolic activity, supporting a normal metabolism and growth pattern for osteoblasts. Positive von Kossa staining demonstrated the presence of bone-like mineral on the SLS materials. Higher hydroxyapatite content composites enhanced cell proliferation, increased alkaline phosphatase activity, and produced more osteocalcin. The present findings showed that SLS materials have good in vitro biocompatibility and hence demonstrated biologically the potential of SLS for medical applications.
authors.affiliation: Department of Materials, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Hao
authors.forename: M M
authors.initials: MM
authors.lastname: Savalani
authors.forename: R A
authors.initials: RA
authors.lastname: Harris
authors.forename: L
authors.initials: L
authors.lastname: Di Silvio
authors.forename: K E
authors.initials: KE
authors.lastname: Tanner
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D007834:Lasers; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology; D015675:Osteocalcin / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism; D010641:Phenotype; D011108:Polymers / Q000494:pharmacology*; D013194:Staining and Labeling; D013499:Surface Properties / Q000187:drug effects; D014966:Xanthenes / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D010078:Oxazines; D011108:Polymers; D014966:Xanthenes; D015675:Osteocalcin; C005843:resazurin; D017886:Durapatite; D000469:Alkaline Phosphatase