title: Expression and changes of hyperoxidized peroxiredoxins in non-pyramidal and polymorphic cells in the gerbil hippocampus during normal aging.
abstract: Oxidative stress is one of predisposing factors to age-related neurodegeneration in the brain. In particular, thiol-containing groups are susceptible to oxidative stress, which induces the formation of the disulfide bond and/or hyperoxidized form of thiol-containing proteins. We observed the protein thiol levels in the hippocampal homogenates and also investigated changes in hyperoxidized form of peroxiredoxin (Prx-SO(3)) immunoreactivity and proteins levels in the gerbil hippocampal subregions during normal aging. Levels of total thiol, non-protein thiol, and protein thiol were decreased in the hippocampal homogenates with age. At post-natal month 1 (PM 1), pyramidal and non-pyramidal cells in the hippocampal CA1 region (CA1) showed Prx-SO(3) immunoreactivity. Prx-SO(3) immunoreactivity in the cells was decreased by PM 12, thereafter, Prx-SO(3) immunoreactivity in the cells increased again with age. In the CA2/3, Prx-SO(3) immunoreactivity in pyramidal cells was not significantly changed; however, the immunoreactivity in pyramidal cells was very low at PM 12. Prx-SO(3) immunoreactivity in the dentate gyrus (DG) was distinctly changed during aging. At PM 1, Prx-SO(3) immunoreactivity in granule and polymorphic cells was weak and strong, respectively. The immunoreactivity in the neurons was decreased with age, not shown in any neurons at PM 12. Thereafter, Prx-SO(3) immunoreactivity increased again with age. In addition, Prx-SO(3) protein level in the hippocampus was lowest at PM 12. These results suggest that thiol-containing proteins are changed during aging and Prx-SO(3) immunoreactivity was different according to cells in the hippocampal subregion during aging.
authors.affiliation: Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea.
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.forename: Jiatian
authors.initials: J
authors.lastname: Yu
authors.forename: Bingchun
authors.initials: B
authors.lastname: Yan
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism; D005849:Gerbillinae / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000378:metabolism*; D017966:Pyramidal Cells / Q000166:cytology / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014020:Tissue Extracts
chemical_list: D013431:Sulfates; D013438:Sulfhydryl Compounds; D014020:Tissue Extracts; D054464:Peroxiredoxins