Chitooligosaccharides suppress the level of protein expression and acetylcholinesterase activity induced by Abeta25-35 in PC12 cells.
Clinical applications of acetylcholinesterase (AChE) inhibitors are widespread in Alzheimer's sufferers in order to activate central cholinergic system and alleviate cognitive deficits by inhibiting the hydrolysis of acetylcholine. In this study, six kinds of chitooligosaccharides (COSs) with different molecular weight and degree of deacetylation were examined for their inhibitory effects against AChE. The 90-COSs exhibited potent AChE inhibitory activities compared to 50-COSs, while 90-MMWCOS (1000-5000 Da) in the 90-COSs showed the highest activity. Cell culture experiment revealed that 90-MMWCOS suppressed the level of AChE protein expression and AChE activity induced by Abeta(25-35) in PC12 cell lines.
Lee
Sang-Hoon
SH
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Park
Jin-Sook
JS
Kim
Se-Kwon
SK
Ahn
Chang-Bum
CB
Je
Jae-Young
JY
D000110:Acetylcholinesterase / Q000378:metabolism; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009844:Oligosaccharides / Q000737:chemistry*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry*; D051381:Rats
D016229:Amyloid beta-Peptides; D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D000110:Acetylcholinesterase