Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses.
Multiphoton excitation by temporally focused pulses can be combined with spatial Fourier-transform pulse shaping techniques to enhance spatial control of the excitation volume. Here we propose and demonstrate an optical system for the generation of such spatiotemporally engineered light pulses using a combination of spatial control by a two-dimensional reconfigurable light modulator, with a dispersive optical setup for temporal focusing. We show that although the properties of a holographic beam significantly differ from those of plane-wave illumination used in previous temporal focusing realizations, this leads only to a slightly reduced axial resolution. We show that the system can provide scanningless, arbitrarily shaped, depth resolved excitation patterns that offer new perspectives for multiphoton photoactivation and optical lithography applications.
Papagiakoumou
Eirini
E
Neurophysiology and New Microscopies Laboratory, Wavefront engineering microscopy group, CNRS UMR8154, INSERM S603, Paris Descartes University, 45, rue des Saints Pères, 75270 Paris Cedex 06, France.
de Sars
Vincent
V
Oron
Dan
D
Emiliani
Valentina
V