Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios.
An aerobic denitrifier was newly isolated from soil and its denitrification activity under different C/N (carbon/nitrogen) ratios was investigated. The isolate was identified as Pseudomonas putida AD-21 by biochemical studies and 16s rDNA sequencing analysis. P. putida AD-21 tolerated oxygen levels of 5.0-6.0 mg/l. An (15)NH(4)/(14)NO(3) analysis indicated that the nitrogen of NH(4) was preferentially assimilated into the cell mass and that the nitrate removed could be considered an indication of bacterial denitrification efficiency. Increasing the C/N ratio increased the nitrate removal rates, whereas nitrogen assimilation into the cell mass was not affected. The optimal C/N ratio was 8 with a maximum nitrate removal rate of 254.6 mg/l/h and a nitrate removal efficiency of 95.9%. The results suggest that P. putida AD-21 may be a good candidate for aerobic wastewater treatment.
Kim
Mia
M
Department of Microbiology, College of Natural Sciences, Pusan National University, Busan, Korea.
Jeong
Seong-Yun
SY
Yoon
Su Jeong
SJ
Cho
Sun Ja
SJ
Kim
Young Hun
YH
Kim
Min Ju
MJ
Ryu
Eun Yoen
EY
Lee
Sang-Joon
SJ
D001420:Bacteria, Aerobic / Q000378:metabolism; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000737:chemistry; D004275:DNA, Ribosomal / Q000378:metabolism*; D004583:Electrons; D004867:Equipment Design; D007218:Industrial Microbiology; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism; D010802:Phylogeny; D016958:Pseudomonas putida / Q000378:metabolism*; D013696:Temperature; D018508:Water Purification
D004275:DNA, Ribosomal; D009566:Nitrates; D002244:Carbon; D009584:Nitrogen; D010100:Oxygen