Neurogenesis induced by seizures in the dentate gyrus is not related to mossy fiber sprouting but is age dependent in developing rats.
Neurogenesis in the dentate gyrus (DG) has attracted attention since abnormal supragranular mossy fiber sprouting occurs in the same region, in temporal lobe epilepsy. Thus, we submitted developing rats to pilocarpine-induced status epilepticus (SE) to study the relationship between neurogenesis and mossy fiber sprouting. Groups were submitted to SE at: I-P9, II-P7, P8 and P9, III-P17 e IV-P21. Neurogenesis was quantified using BrdU protocol and confirmed through double staining, using neuronal pentraxin. Other animals were monitored by video system until P120 and their brain was studied (Timm and Nissl staining). The neurogenesis at P17 (p=0.007) and P21 (p=0.006) were increased. However, only P21 group showed recurrent seizures and the mossy fiber sprouting in the same region, during adult life, while P17 did not. Thus, our results suggest that neurogenesis is not related to mossy fiber sprouting neither to recurrent spontaneous seizures in pilocarpine model.
Sanabria
Yaima del Carmen Garrido
Ydel C
Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo SP, Brasil.
Argañaraz
Gustavo Adolfo
GA
Lima
Eliangela
E
Priel
Margareth Rose
MR
Trindade
Edvaldo da Silva
Eda S
Loeb
Luana Mazzacoratti
LM
Scorza
Fulvio Alexandre
FA
Cavalheiro
Esper Abrão
EA
Amado
Débora
D
Naffah-Mazzacoratti
Maria da Graça
Mda G
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D018891:Dentate Gyrus / Q000187:drug effects / Q000196:embryology / Q000503:physiopathology*; D007150:Immunohistochemistry; D019599:Mossy Fibers, Hippocampal / Q000187:drug effects / Q000196:embryology / Q000503:physiopathology; D055495:Neurogenesis / Q000502:physiology*; D009473:Neuronal Plasticity; D010862:Pilocarpine; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000139:chemically induced / Q000503:physiopathology*
D010862:Pilocarpine