title: [Adenoviral-mediated APE/Ref-1 expression protects rat spiral ganglion cells from oxidative damage].
abstract: OBJECTIVE
  To address the question if apurinic/apyrimidinic endonuclease/redox factor 1 (APE/Ref-1) involved in preventing spiral ganglion cells oxidative damage after oxidative stress.
  
  
  METHODS
  Primary cultured rat spiral ganglion cells were infected with the adenovirus containing APE/Ref-1 for 48 h, then treated with H2O2 (0, 10, 25, 50, 100, 300 micromol/L) for 1 h, and finally changed back into normal medium. Western blot were used to detect the level of APE/Ref-1 protein in the infected cells to ensure APE/Ref-1 over expression as a result of adenovirus infection. The cell viability was determined by MTT and the apoptosis of spiral ganglion cells was determined by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).
  
  
  RESULTS
  Western blot showed that infection of adenovirus resulted in APE/Ref-1 over expression in the spiral ganglion cells. Over expression of APE/Ref-1 significantly improved cell viability in cultures treated with different concentration H2O2 from 50 to 300 micromol/L However, the apoptosis of cells was significantly inhibited.
  
  
  CONCLUSIONS
  Over expression of APE/Ref-1 could protect spiral ganglion cells from oxidative damage.
authors.affiliation: Department of Otorhinolarynogology Head and Neck Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhen-dong
authors.initials: ZD
authors.lastname: Jiang
authors.forename: Xue-yuan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Yun-jun
authors.initials: YJ
authors.lastname: Wei
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhong
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000473:pathology*
chemical_list: D006861:Hydrogen Peroxide; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase