title: Cytotoxic activity of alkaloids isolated from Stephania rotunda [corrected].
abstract: Three major alkaloids: cepharanthine (1), tetrahydropalmatine (2) and xylopinine (3) isolated from Stephania rotunda tuber were investigated for their cytotoxic activity in a panel of human cancer cells (HT29, LS174T, SW620 and HepG2) using MTT assay. In the present study, cepharanthine (1) exerted potent cytotoxicity against colon and hepatoma cancer cell lines with IC(50) values between 2.4 and 5.3 microM while tetrahydropalmatine (2) and xylopinine (3) displayed weak cytotoxicity. In addition, the mutagenic activity of cepharanthine (1) was investigated using a modified liquid incubation technique of the Salmonella/microsomal assay. This alkaloid (1) was found to be non-mutagenic for doses up to 8.2 microM.
authors.affiliation: Laboratory of Pharmacognosy, UMR-MD3, Faculty of Pharmacy, Marseille, France. siya.bun@pharmacie.univ-mrs.fr
authors.forename: Sok-Siya
authors.initials: SS
authors.lastname: Bun
authors.forename: Michèle
authors.initials: M
authors.lastname: Laget
authors.forename: Aun
authors.initials: A
authors.lastname: Chea
authors.forename: Hot
authors.initials: H
authors.lastname: Bun
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ollivier
authors.forename: Riad
authors.initials: R
authors.lastname: Elias
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D012486:Salmonella typhimurium / Q000187:drug effects; D031607:Stephania / Q000737:chemistry*; D052117:Benzodioxoles
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; C453820:xylopinine; C014215:tetrahydropalmatine; C006947:cepharanthine; D052117:Benzodioxoles