title: Surveillance of stool samples for the presence of Enterobacter sakazakii among Korean people.
abstract: OBJECTIVE
  Enterobacter sakazakii (E. sakazakii) infections are an important cause of life-threatening meningitis, septicemia, and necrotizing enterocolitis in infants. Dried infant formula milk is an important vehicle for E. sakazakii infection. E. sakazakii was isolated in Korea from dried infant formula milk. Although E. sakazakii infection of infants may occur in Korea, its prevalence has not yet been documented. Therefore, we determined the prevalence of E. sakazakii and documented symptoms.
  
  
  METHODS
  Between March and October 2006, 1,146 stool samples were collected from patients at Uijeongbu St. Mary's Hospital. Each fecal swab was dissolved in 10mL of buffered peptone solution, and enriched culture was streaked onto Druggan-Forsythe-Iversen (DFI) agar. Presumptive E. sakazakii colonies that exhibited a blue-green color during culture on DFI medium were selected. The identity of colonies that developed yellow pigment during culture on TSA was determined using the Vitek system and PCR.
  
  
  RESULTS
  We isolated 4 E. sakazakii strains whose 16S rRNA sequence alignments had a similarity of 99% with those of 3 E. sakazakii ATCC strains.
  
  
  CONCLUSIONS
  This is the first report on isolation of E. sakazakii from stool samples and to document the symptoms of Korean patients.
authors.affiliation: Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment, Suwon, Korea.
authors.forename: Jung Beom
authors.initials: JB
authors.lastname: Kim
authors.forename: Seung-Hak
authors.initials: SH
authors.lastname: Cho
authors.forename: Yong Bae
authors.initials: YB
authors.lastname: Park
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Jong Chan
authors.initials: JC
authors.lastname: Kim
authors.forename: Bok Kwon
authors.initials: BK
authors.lastname: Lee
authors.forename: Hae Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Hiun Suk
authors.initials: HS
authors.lastname: Chae
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D044083:Cronobacter sakazakii / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D055815:Young Adult
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S