Synthesis, characterization, DNA interaction and cytotoxic activities of copper complexes with ethyl 2-[bis(2-pyridylmethyl)amino]propionate.
Copper(II) complexes of ethyl 2-[bis(2-pyridylmethyl)amino]propionate ligand (ETDPA) have been synthesized and characterized by elemental analyses, IR spectra, UV spectra and ES-MS. These complexes are stable in air with the formula of [(ETDPA)CuCl(2)] and [(ETDPA)Cu(phen)](ClO(4))(2). The DNA-binding properties of the Cu(II) complexes have been investigated by ultraviolet spectroscopy and fluorescence spectroscopy, which showed that the binding mode of the two complexes with DNA was different. The binding mode of [(ETDPA)CuCl(2)] is not the classical intercalation binding, and the binding mode of [(ETDPA)Cu(phen)](ClO(4))(2) with DNA is intercalation. The cytotoxic assay shows that the [(ETDPA)Cu(phen)](ClO(4))(2) is more active on the proliferation of cancer cells than the [(ETDPA)CuCl(2)].
Chen
Qiu-Yun
QY
Institute of Chemistry and Chemical Engineer, Jiangsu University, Zhenjiang 212013, PR China; State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 212093, PR China. chenqy@ujs.edu.cn
Huang
Juan
J
Guo
Wen-Jie
WJ
Gao
Jing
J
D000818:Animals; D002021:Buffers; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry* / Q000633:toxicity*; D004247:DNA / Q000378:metabolism*; D005562:Formazans / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D011422:Propionates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity; D013696:Temperature; D013778:Tetrazolium Salts / Q000378:metabolism; D013816:Thermodynamics; D014732:Vibration
D002021:Buffers; D005562:Formazans; D008024:Ligands; D011422:Propionates; D013778:Tetrazolium Salts; C079782:MTT formazan; D003300:Copper; D004247:DNA; C427203:calf thymus DNA