Removal of estrogenic activity of iso-butylparaben and n-butylparaben by laccase in the presence of 1-hydroxybenzotriazole.
In the presence of a redox mediator, 1-hydroxybenzotriazole (HBT), iso-butylparaben (iso-BP) and n-butylparaben (n-BP) were treated with laccase from white rot fungus Trametes versicolor. HPLC analysis demonstrated that iso-BP and n-BP almost completely disappeared from the reaction mixture after 4 h of treatment with the laccase-HBT system. Using the yeast two-hybrid assay system, it was also confirmed that the laccase-HBT system substantially removed the estrogenic activity of iso-BP and n-BP after 4 h of treatment. Furthermore, there was a linear relationship between the removal of estrogenic activity of both parabens and the decrease in their concentrations. These results demonstrate that the laccase-HBT system is effective in eliminating iso-BP and n-BP, and removing the estrogenic activity of both parabens.
Mizuno
Hirohito
H
Faculty of Agriculture, Shizuoka University, Surugaku, Shizuoka, Japan.
Hirai
Hirofumi
H
Kawai
Shingo
S
Nishida
Tomoaki
T
D001487:Basidiomycota / Q000201:enzymology* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000302:isolation & purification*; D042845:Laccase / Q000378:metabolism*; D010226:Parabens / Q000302:isolation & purification*; D014230:Triazoles / Q000378:metabolism*
D004967:Estrogens; D010226:Parabens; D014230:Triazoles; C471067:isobutylparaben; C011852:1-hydroxybenzotriazole; D042845:Laccase