A new 1'-methylenedisulfide deoxyribose that forms an efficient cross-link to DNA cytosine-5 methyltransferase (DNMT).
A newly designed 1'-methylenedisulfide deoxyribose probe can efficiently cross-link to DNA cytosine-5-methyltransferases (DNMTs) through the active Cys residue, which provides a new tool to covalently trap DNMT-DNA complexes.
Shigdel
Uddhav Kumar
UK
Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
He
Chuan
C
D003432:Cross-Linking Reagents / Q000737:chemistry; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry* / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry; D015257:DNA-Cytosine Methylases / Q000737:chemistry / Q000378:metabolism; D003855:Deoxyribose / Q000031:analogs & derivatives* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D012436:S-Adenosylmethionine / Q000737:chemistry
D003432:Cross-Linking Reagents; D015342:DNA Probes; D004277:DNA, Single-Stranded; D004220:Disulfides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D003855:Deoxyribose; D012436:S-Adenosylmethionine; C037664:DNA modification methylase HhaI; D015257:DNA-Cytosine Methylases; D004248:DNA (Cytosine-5-)-Methyltransferases