Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo.
Understanding the diverse activities of the multisubunit core promoter recognition complex TFIID in vivo requires knowledge of how individual subunits contribute to overall functions of this TATA box-binding protein (TBP)/TBP-associated factor (TAF) complex. By generating altered holo-TFIID complexes in Drosophila we identify the ETO domain of TAF4 as a coactivator domain likely targeted by Pygopus, a protein that is required for Wingless-induced transcription of naked cuticle. These results establish a coactivator function of TAF4 and provide a strategy to dissect mechanisms of TFIID function in vivo.
Wright
Kevin J
KJ
Howard Hughes Medical Institute, Li Ka-Shing Center for Biomedical and Sciences, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Tjian
Robert
R
D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007814:Larva; D046912:Multiprotein Complexes; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D015398:Signal Transduction; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000378:metabolism*; D014157:Transcription Factors; D051153:Wnt Proteins / Q000378:metabolism*
D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046912:Multiprotein Complexes; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; C082222:RUNX1T1 protein, human; C101882:TAF4 protein, human; D035381:TATA-Binding Protein Associated Factors; C103338:Taf4 protein, Drosophila; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D051153:Wnt Proteins; C453400:pygo protein, Drosophila