title: Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus.
abstract: A new grapevine leafroll-associated virus isolate (GLRaV-Pr) from Greek grapevines was recently reported. This virus, along with the genetically related GLRaV-4, -5, -6 and -9, form a separate diverse lineage within the genus Ampelovirus. In this paper, the complete nucleotide sequence of GLRaV-Pr was determined, making it the first fully sequenced virus of this lineage. Its genome is 13,696 nt long and contains seven open reading frames, which potentially encode a 253-kDa polyprotein containing papain-like protease, methyltransferase, AlkB and helicase domains, a 58.2-kDa RNA-dependent RNA polymerase, a 5.2-kDa hydrophobic protein, a 58.5-kDa heat shock 70 protein homologue, a 60-kDa protein, a 30-kDa coat protein (CP) and a 23-kDa protein. A virus-specific antibody was raised against the recombinant CP of GLRaV-Pr and was applied in western blot analysis. The genomic, serological and phylogenetic data reported here confirm that GLRaV-Pr is a member of a distinct Ampelovirus species. Comparisons of GLRaV-Pr with the only available genetically related, fully sequenced virus, PMWaV-1, PBNSPaV and the partially sequenced GLRaV-9 revealed that this lineage, including GLRaV-4, -5, -6, -9 and -De, exhibits a high uniformity of genome organization and includes the smallest and simplest viruses within the family Closteroviridae.
authors.affiliation: Plant Pathology Laboratory, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. vmaliogk@agro.auth.gr
authors.forename: Varvara I
authors.initials: VI
authors.lastname: Maliogka
authors.forename: C I
authors.initials: CI
authors.lastname: Dovas
authors.forename: L
authors.initials: L
authors.lastname: Lotos
authors.forename: K
authors.initials: K
authors.lastname: Efthimiou
authors.forename: N I
authors.initials: NI
authors.lastname: Katis
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D029381:Closteroviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D003546:Cysteine Endopeptidases / Q000235:genetics; D016679:Genome, Viral; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017434:Protein Structure, Tertiary / Q000235:genetics; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017421:Sequence Analysis; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D027843:Vitis / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D018840:HSP70 Heat-Shock Proteins; D014764:Viral Proteins; D006899:Mixed Function Oxygenases; D008780:Methyltransferases; D012324:RNA-Dependent RNA Polymerase; D003546:Cysteine Endopeptidases; D020365:RNA Helicases