title: Molecular characterization of ORFs 2 to 7 of Korean porcine reproductive and respiratory syndrome virus (CA) and its protein expression by recombinant baculoviruses.
abstract: To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.
authors.affiliation: Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
authors.forename: Hyun Na
authors.initials: HN
authors.lastname: Koo
authors.forename: Jeong Mi
authors.initials: JM
authors.lastname: Oh
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Lee
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.forename: Jong Yul
authors.initials: JY
authors.lastname: Roh
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Sung Sik
authors.initials: SS
authors.lastname: Yoo
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Young In
authors.initials: YI
authors.lastname: Kim
authors.forename: In Joong
authors.initials: IJ
authors.lastname: Yoon
authors.forename: Soo Dong
authors.initials: SD
authors.lastname: Woo
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002478:Cells, Cultured; D007723:Korea; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011995:Recombination, Genetic; D016415:Sequence Alignment; D018411:Spodoptera / Q000821:virology; D013552:Swine; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015678:Viral Structural Proteins