New 18-l process-scale counter-current chromatography centrifuge.
A new Dynamic Extractions Maxi-counter-current chromatography (CCC) centrifuge with a column volume of 18-l has been installed in the Advanced Bioprocessing Centre at Brunel. This instrument has four times the capacity of the 4.6-l Maxi-CCC centrifuge which has been operating robustly for 3 years. Tests using the model sample system benzyl alcohol and p-cresol with a heptane:ethyl acetate:methanol:water (HEMWat) phase system (1.4:0.1:0.5:1.0) show that resolution is almost double with this new high capacity device. Commissioning tests with a mixture of caffeine, K(D)=0.21; ferulic acid, K(D)=0.82; umbelliferone, K(D)=1.2 and vanillin, K(D)=1.49 using a HEMWat phase system of 1:1.5:1:1.5 on the 9-l column show that resolutions equivalent to analytical instruments will be possible using the full 18-l capacity. They also show that predictable scale-up from simple test tube tests is feasible with knowledge of the stationary phase retention for the planned process scale run.
Sutherland
Ian
I
Brunel Institute for Bioengineering, Brunel University, Uxbridge, Middlesex, UK. ian.sutherland@brunel.ac.uk
Hewitson
Peter
P
Ignatova
Svetlana
S
D001547:Benzaldehydes / Q000737:chemistry; D019905:Benzyl Alcohol / Q000737:chemistry; D002110:Caffeine / Q000737:chemistry; D002498:Centrifugation / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods; D003408:Cresols / Q000737:chemistry; D004867:Equipment Design; D008962:Models, Theoretical; D012997:Solvents / Q000737:chemistry; D014468:Umbelliferones / Q000737:chemistry; D014584:User-Computer Interface
D001547:Benzaldehydes; D003373:Coumaric Acids; D003408:Cresols; D012997:Solvents; D014468:Umbelliferones; C032538:4-cresol; D002110:Caffeine; C031477:7-hydroxycoumarin; C004999:ferulic acid; C100058:vanillin; D019905:Benzyl Alcohol