title: Correct charge state assignment of native electrospray spectra of protein complexes.
abstract: Correct charge state assignment is crucial to assigning an accurate mass to supramolecular complexes analyzed by electrospray mass spectrometry. Conventional charge state assignment techniques fall short of reliably and unambiguously predicting the correct charge state for many supramolecular complexes. We provide an explanation of the shortcomings of the conventional techniques and have developed a robust charge state assignment method that is applicable to all spectra.
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA.
authors.forename: Lars
authors.initials: L
authors.lastname: Liepold
authors.forename: Luke M
authors.initials: LM
authors.lastname: Oltrogge
authors.forename: Peter A
authors.initials: PA
authors.lastname: Suci
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Young
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
mesh_terms: D000465:Algorithms; D008956:Models, Chemical; D046912:Multiprotein Complexes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D046912:Multiprotein Complexes; D011506:Proteins