[Identification of human coronary artery endothelial cells obtained by coronary endovascular biopsy].
OBJECTIVE
To develop a method to obtain and identify human coronary artery endothelial cells obtained during percutaneous coronary interventions (PCI).


METHODS
Coronary guide wires were used to obtain endothelial cells from coronary arteries in 28 patients undergoing PCI. The cells were eluted from the wire tips and then purified by magnetic beads coated with anti-CD146 antibody. von Willebrand factor (vWF) was used as an immunocytochemical marker for endothelial cells. The cellular viability was evaluated by observing cell membrane integrity and energy-dependent uptake of DiI-labeled acetylated low-density lipoprotein.


RESULTS
An average of 96 coronary artery endothelial cells with good viability per patient were obtained by one guide wire. vWF identification showed their endothelial morphology and immunoreactivity.


CONCLUSIONS
The viable coronary endothelial cells could be obtained during routine percutaneous coronary interventions combined with magnetic beads isolation technique. These cells may be used for further cellular functional analyses (such as immunocytochemistry and molecular biology) and expand our understanding on mechanisms of coronary artery diseases.
Yu
Shi-Yong
SY
Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Huang
Lan
L
Song
Yao-Ming
YM
Li
Ai-Min
AM
Qin
Jun
J
Yu
Xue-Jun
XJ
Geng
Zhao-Hua
ZH
Zhao
Gang
G
Lin
Chun-Mei
CM
Tao
Chun-Rong
CR
D001706:Biopsy / Q000379:methods; D003331:Coronary Vessels / Q000166:cytology / Q000473:pathology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged