title: [Identification of human coronary artery endothelial cells obtained by coronary endovascular biopsy].
abstract: OBJECTIVE
  To develop a method to obtain and identify human coronary artery endothelial cells obtained during percutaneous coronary interventions (PCI).
  
  
  METHODS
  Coronary guide wires were used to obtain endothelial cells from coronary arteries in 28 patients undergoing PCI. The cells were eluted from the wire tips and then purified by magnetic beads coated with anti-CD146 antibody. von Willebrand factor (vWF) was used as an immunocytochemical marker for endothelial cells. The cellular viability was evaluated by observing cell membrane integrity and energy-dependent uptake of DiI-labeled acetylated low-density lipoprotein.
  
  
  RESULTS
  An average of 96 coronary artery endothelial cells with good viability per patient were obtained by one guide wire. vWF identification showed their endothelial morphology and immunoreactivity.
  
  
  CONCLUSIONS
  The viable coronary endothelial cells could be obtained during routine percutaneous coronary interventions combined with magnetic beads isolation technique. These cells may be used for further cellular functional analyses (such as immunocytochemistry and molecular biology) and expand our understanding on mechanisms of coronary artery diseases.
authors.affiliation: Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Yu
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.forename: Yao-Ming
authors.initials: YM
authors.lastname: Song
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Yu
authors.forename: Zhao-Hua
authors.initials: ZH
authors.lastname: Geng
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Lin
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Tao
mesh_terms: D001706:Biopsy / Q000379:methods; D003331:Coronary Vessels / Q000166:cytology / Q000473:pathology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged