title: Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2.
abstract: Manganese-dependent superoxide dismutase (SOD2) serves as the primary defense against mitochondrial superoxide, and decreased SOD2 activity results in a range of pathologies. To investigate the events occurring soon after depletion of SOD2, we generated SOD2 gene knockout chicken DT40 cells complemented with a human SOD2 (hSOD2) cDNA, whose expression can be switched off by doxycycline (Dox). When SOD2 was depleted by the addition of Dox, the cells grew slightly slower and formed fewer colonies than cells expressing hSOD2. In addition, these cells showed a high sensitivity to paraquat, which produces superoxide, and died through apoptosis. In contrast to results obtained with mouse and DrosophilaSod2 mutants, we found no indication of an increase in DNA lesions due to depletion of SOD2.
authors.affiliation: Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Shunya
authors.initials: S
authors.lastname: Takada
authors.forename: Eri
authors.initials: E
authors.lastname: Inoue
authors.forename: Keizo
authors.initials: K
authors.lastname: Tano
authors.forename: Hanako
authors.initials: H
authors.lastname: Yoshii
authors.forename: Takuya
authors.initials: T
authors.lastname: Abe
authors.forename: Akari
authors.initials: A
authors.lastname: Yoshimura
authors.forename: Motomu
authors.initials: M
authors.lastname: Akita
authors.forename: Shusuke
authors.initials: S
authors.lastname: Tada
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Seki
authors.forename: Takemi
authors.initials: T
authors.lastname: Enomoto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics; D002645:Chickens; D004249:DNA Damage / Q000235:genetics; D055786:Gene Knockout Techniques; D005816:Genetic Complementation Test; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D009154:Mutation; D010269:Paraquat / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000235:genetics*; D013481:Superoxides / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D010269:Paraquat; D013482:Superoxide Dismutase; D013481:Superoxides; D000099156:Superoxide Dismutase 2