Prenylisoflavonoids from Erythrina senegalensis as novel HIV-1 protease inhibitors.
Eight compounds were isolated from the CH (2)Cl (2) extracts of ERYTHRINA SENEGALENSIS to assess HIV-1 protease (PR) activity inhibition. The prenylated isoflavone structures, identified by spectroscopic analysis, were 8-prenylluteone ( 1), auriculatin ( 2), erysenegalensein O ( 3), erysenegalensein D ( 4), erysenegalensein N ( 5), derrone ( 6), alpinumisoflavone ( 7), and 6,8-diprenylgenistein ( 8). The constituents showed dose-dependent inhibitory activities on HIV-1 PR with IC (50) values from 0.5 to 30.0 muM. Compounds 1 - 5 possessing two hydroxy groups in the 2' and 4' positions of the B ring, potently inhibited HIV-1 PR activity. In addition, 6,8-diprenylgenistein ( 8) with two prenyl groups in the 6 and 8 positions of the A ring and one hydroxy group in the 4' position of B-ring was the most potent HIV-1 PR inhibitor.
Lee
Jisuk
J
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Kwanak-gu, Seoul, Korea. jslee103@snu.ac.kr
Oh
Won Keun
WK
Ahn
Jong Seog
JS
Kim
Yong Hae
YH
Mbafor
J Tanyi
JT
Wandji
Jean
J
Fomum
Z Tanee
ZT
D004305:Dose-Response Relationship, Drug; D004895:Erythrina / Q000737:chemistry*; D017320:HIV Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems
D017320:HIV Protease Inhibitors; D007529:Isoflavones; D010936:Plant Extracts