title: Physicochemical and hypocholesterolemic characterization of oxidized oat beta-glucan.
abstract: 2,2,6,6-Tetramethyl-1-piperidine oxoammonium ion (TEMPO)-mediated oxidation was applied to oat beta-glucans, and the physicochemical and hypocholesterolemic properties of the resulting derivatives were investigated. The (13)C NMR spectra revealed that C6 primary alcohol groups were selectively oxidized into carboxyl groups. The oxidized derivatives exhibited enhanced water solubility and improved in vitro bile acid binding capacity. When hypercholesterolemic rats were fed diets containing the oxidized beta-glucan, the levels of triglyceride, total cholesterol, LDL-C, and VLDL-C in the rats significantly decreased (p < 0.05), consequently improving the serum lipid profiles. Dietary supplementation with beta-glucans reduced also the total cholesterol level in liver. Furthermore, more fecal eliminations of total cholesterol and triglyceride were observed, which were favorably correlated to their reduced levels in the serum and liver. As a result, oxidized oat beta-glucan exhibits potential use as an active cholesterol-lowering ingredient.
authors.affiliation: Department of Food and Nutrition, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, Korea.
authors.forename: Seung Young
authors.initials: SY
authors.lastname: Park
authors.forename: In Young
authors.initials: IY
authors.lastname: Bae
authors.forename: Suyong
authors.initials: S
authors.lastname: Lee
authors.forename: Hyeon Gyu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000737:chemistry* / Q000494:pharmacology; D018554:Avena / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D005243:Feces / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D014280:Triglycerides / Q000032:analysis / Q000097:blood; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000924:Anticholesteremic Agents; D014280:Triglycerides; D047071:beta-Glucans; D002784:Cholesterol