title: [The effects of extracts from Arenaria kansuensis on the levels of HBsAG and HBeAg in HepG2.2.15 cells].
abstract: OBJECTIVE
  To investigate the anti-HBV effects of the extracts with alcohol and water from Arenaria kansuensis in vitro.
  
  
  METHODS
  HepG2.2.15 cells were used to estimate the effects of the extracts with alcohol and water from Arenaria kansuensis on the levels of HBsAg and HBeAg in medium by ELISA.
  
  
  RESULTS
  Incubation of HepG2.2.15 cells with both the extracts with alcohol and water from Arenaria kansuensis (50-400 mg/L) for 72 and 96h significantly inhibited the levels of HBsAg and HBeAg. The maximum inhibiting rate of HBeAg from the Arenaria kansuensis extracts with alcohol was 63.2% and 90. 8% at 72h and 96h, respectively. Meanwhile, the Arenaria kansuensis extracts with water had obvious inhibitory effects on the levels of HBsAg and HBeAg in HepG2.2.15 cells at 96 h, compared with that of the control group (P < 0.01). The maximum inhibiting rates were 52.5% and 72.8%, respectively.
  
  
  CONCLUSIONS
  The extracts with water from Arenaria kansuensis has obvious anti-HBV effects in vitro.
authors.affiliation: The 181st hospital of P. L. A, Guilin 541002, China.
authors.forename: Qing-nian
authors.initials: QN
authors.lastname: Tang
authors.forename: Mei
authors.initials: M
authors.lastname: Feng
authors.forename: Shou-ji
authors.initials: SJ
authors.lastname: Gong
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Dai
authors.forename: Yi-zhi
authors.initials: YZ
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Xv
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D029748:Caryophyllaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D010936:Plant Extracts; D014867:Water; D000431:Ethanol