Hydrogen sulphide is pro-inflammatory in haemorrhagic shock.
OBJECTIVE
H(2)S is pro-inflammatory in inflammatory models, thus we investigated whether H(2)S plays a role in haemorrhagic shock (HS)-associated inflammation.


METHODS
Male, Sprague-Dawley rats were given an inhibitor of H(2)S biosynthesis, DL-propargylglycine (PAG, 50 mg/kg, i. v.) or saline (1 ml/kg) 30 min before blood withdrawal and subjected to HS (mean arterial pressure (MAP) of 40 mM Hg for 90 min) followed by reinfusion of shed blood. Animals were killed at 5, 90, 270 and 630 min after reinfusion.


RESULTS
Pre-treatment of animals with PAG 1) increased the HR recovery rate (n = 6 - 12, P < 0.05); 2) attenuated the increase in plasma levels of TNF-alpha and IL-6 and reduced lung iNOS expression levels (n =5 P, < 0.05); and 3) attenuated the increase in plasma levels of ALT and reduced HS-induced increase in liver and lung myeloperoxidase (MPO) activity (n = 5, P < 0.05).


CONCLUSIONS
H(2)S is pro-inflammatory in HS and inhibition of H(2)S biosynthesis may reduce some HS-induced inflammatory responses and organ injury.
Mok
Y-Y P
YY
Institute of Medical Biology, 8A Biomedical Grove, #05-06 Immunos, Singapore, Singapore, 138648. pamela.mok@imb.a-star.edu.sg
Moore
P K
PK
D000480:Alkynes / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006339:Heart Rate / Q000187:drug effects; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007249:Inflammation / Q000209:etiology*; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000150:complications* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000480:Alkynes; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C009055:propargylglycine; D005998:Glycine; D006862:Hydrogen Sulfide