No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions.
GPRC6A is a seven-transmembrane receptor mediating signaling by a wide range of L-alpha-amino acids, a signaling augmented by the divalent cations Ca(2)(+) and Mg(2)(+). GPRC6A transcripts are detected in numerous mammalian tissues, but the physiological role of the receptor is thus far elusive. Analogously to the closely related calcium-sensing receptor, GPRC6A has been proposed to function as a metabolic sensor of Ca(2)(+) and amino acids in bone and other tissues. In the present study, we have generated the first GPRC6A knockout mice and studied their phenotype with particular focus on bone homeostasis. The generated GPRC6A knockout mice are viable and fertile, develop normally, and exhibit no significant differences in body weight compared with wild-type littermates. Assessment of bone mineral density, histomorphometry, and bone metabolism demonstrated no significant differences between 13-week-old knockout and wild-type mice. In conclusion, our data do not support a role for GPRC6A in normal bone physiology.
Wellendorph
Petrine
P
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark. pw@farma.ku.dk
Johansen
Lars Dan
LD
Jensen
Anders A
AA
Casanova
Emilio
E
Gassmann
Martin
M
Deprez
Pierre
P
Clément-Lacroix
Philippe
P
Bettler
Bernhard
B
Bräuner-Osborne
Hans
H
D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D005838:Genotype; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D010641:Phenotype; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013977:Tibia / Q000033:anatomy & histology / Q000378:metabolism
C515750:GPRC6A protein, mouse; D043562:Receptors, G-Protein-Coupled