Effect of several flavonoid-containing plant preparations on activity of mitochondrial ATP-dependent potassium channel.
Flavonoid-containing plant preparations (water soluble extracts of Pentaphylloides fruticosa [Extralife], Emblica officinalis Gaerth [Amla], and Bergenia crassifolia [Bergenia]) produced a dose-dependent and tissue-specific effect on activity of mitochondrial ATP-dependent potassium channel. The effect of these preparations was biphasic (activation and inhibition). The activating effect of Extralife was one order of magnitude higher than that of Amla and Bergenia and was observed in a wider concentration range. The activating effect of preparations was abolished by inhibitors of the mitochondrial ATP-dependent potassium channel, which attested to specificity of their influence on mitochondrial channel. Under in vivo conditions, the antihypoxic effect of Extralife was partially abolished by mitochondrial ATP-dependent potassium channel inhibitor 5-hydroxydecanoate.
Mironova
G D
GD
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Shigaeva
M I
MI
Belosludtseva
N V
NV
Gritsenko
E N
EN
Belosludtsev
K N
KN
Germanova
E L
EL
Lukyanova
L D
LD
D000818:Animals; D015687:Cell Hypoxia; D003652:Decanoic Acids / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D006880:Hydroxy Acids / Q000494:pharmacology; D054086:KATP Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D029842:Phyllanthus emblica; D010936:Plant Extracts / Q000494:pharmacology*; D011187:Posture; D051381:Rats
D003652:Decanoic Acids; D005419:Flavonoids; D006880:Hydroxy Acids; D054086:KATP Channels; D010936:Plant Extracts; C527119:extralife; C052853:5-hydroxydecanoic acid