Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae).
BACKGROUND
Several in vitro studies have looked at the effect of medicinal plant extracts against Helicobacter pylori (H. pylori). Regardless of the popular use of Byrsonima crassa (B. crassa) as antiemetic, diuretic, febrifuge, to treat diarrhea, gastritis and ulcers, there is no data on its effects against H. pylori. In this study, we evaluated the anti-H. pylori of B. crassa leaves extracts and its effects on reactive oxygen/nitrogen intermediates induction by murine peritoneal macrophages.


METHODS
The minimal inhibitory concentration (MIC) was determined by broth microdilution method and the production of hydrogen peroxide (H2O2) and nitric oxide (NO) by the horseradish peroxidase-dependent oxidation of phenol red and Griess reaction, respectively.


RESULTS
The methanolic (MeOH) and chloroformic (CHCl3) extracts inhibit, in vitro, the growth of H. pylori with MIC value of 1024 microg/ml. The MeOH extract induced the production H2O2 and NO, but CHCl3 extract only NO.


CONCLUSIONS
Based in our results, B. crassa can be considered a source of compounds with anti-H. pylori activity, but its use should be done with caution in treatment of the gastritis and peptic ulcers, since the reactive oxygen/nitrogen intermediates are involved in the pathogenesis of gastric mucosal injury induced by ulcerogenic agents and H. pylori infections.
Bonacorsi
Cibele
C
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rua Expedicionários do Brasil 1621, CEP14801-960, Araraquara, SP, Brazil. bonac@terra.com.br
Raddi
Maria Stella G
MS
Carlos
Iracilda Z
IZ
Sannomiya
Miriam
M
Vilegas
Wagner
W
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016481:Helicobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D029581:Malpighiaceae / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D009569:Nitric Oxide; D006861:Hydrogen Peroxide