title: Differential protein expression in heart in UT-B null mice with cardiac conduction defects.
abstract: Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at different ages. By two-dimensional electrophoresis-based comparative analysis, we found that more than dozen proteins were modulated (>two-fold) in the myocardium of UT-B null mice. Out of these modulated proteins, troponin T (TNNT2) presented significant changes in UT-B null mice at early stage prior to the development of P-R interval elongation, while the change of atrial natriuretic peptide (ANP) occurred only at late stage in UT-B null mice that had the AV block. These data indicate that UT-B deletion caused the dynamic expression regulation of TNNT2 and ANP, and these proteins may provide new clues to investigate the molecular events involved in cardiac conduction.
authors.affiliation: Department of Pathophysiology, Research Center of Prostate Diseases, School of Basic Medicine, Jilin University, Changchun, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Meng
authors.forename: Li-Shun
authors.initials: LS
authors.lastname: Wang
authors.forename: Xian
authors.initials: X
authors.lastname: Jin
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Kun
authors.initials: K
authors.lastname: Ji
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.forename: Xue-Jian
authors.initials: XJ
authors.lastname: Zhao
authors.forename: Baoxue
authors.initials: B
authors.lastname: Yang
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006329:Heart Conduction System / Q000378:metabolism* / Q000473:pathology*; D066298:In Vitro Techniques; D026901:Membrane Transport Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020107:Troponin T / Q000378:metabolism; D000096982:Urea Transporters
chemical_list: D009320:Atrial Natriuretic Factor; D026901:Membrane Transport Proteins; D020107:Troponin T; D000096982:Urea Transporters