title: Integrating waveguide biosensor.
abstract: The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed using Salmonella as model. The analyte was immunocaptured on the inner surface of the waveguide and then detected by the antibody conjugated with fluorescent dye. The waveguide was illuminated by an excitation light at a 90 degrees angle. The emitted light from fluorescent labels on the surface of the waveguide was efficiently collected and channeled to a detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing fluorescent dye Cy5, a 635-nm diode laser for excitation, and a photomultiplier tube detector, the Integrating Waveguide Sensor System was able to detect approximately ten captured cells of Salmonella.
authors.affiliation: Creatv MicroTech Inc, Potomac, MD, USA.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Li
authors.forename: Platte
authors.initials: P
authors.lastname: Amstutz
authors.forename: Cha-Mei
authors.initials: CM
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Hang
authors.forename: Peixuan
authors.initials: P
authors.lastname: Zhu
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Shelton
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Karns
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055096:Optical Devices; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D018511:Systems Integration