Validation of a sensitive gas chromatographic-mass spectrometric method for the simultaneous determination of beta-elemene and beta-elemenal in human plasma.
A sensitive gas chromatographic-mass spectrometric assay was described for determination of beta-elemene and beta-elemenal in human plasma, which has been successfully applied in clinical trial. After liquid-liquid extraction and gas chromatographic separation, the analytes were identified and quantitated. Calibration curves were linear in range from 31.25 to 8000 ng mL(-1) and the limit of quantification for both was 31.25 ng mL(-1). Intra- and inter-day precision at three concentrations were 2.3-8.3% with accuracy of -8.5 to 6.1% for elemene and 3.0-9.9% with accuracy of -2.3 to 5.9% for elemenal. The method was validated to be suitable for further pharmacokinetic study.
Chen
Zhihang
Z
State Key Laboratory of Pathogen and Biosecurity, Beijing, PR China.
Song
Yanxia
Y
Che
Jinjing
J
Liu
Xiaolei
X
Ning
Yu
Y
Shan
Chengqi
C
Hou
Yunan
Y
Liu
Yunlong
Y
Miao
Xiaohong
X
Cheng
Yuanguo
Y
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000097:blood*
D012717:Sesquiterpenes; C445979:beta-elemene