title: Lactobacillus reuteri prevents colitis by reducing P-selectin-associated leukocyte- and platelet-endothelial cell interactions.
abstract: Recent findings indicate that dextran sodium sulfate (DSS)-induced colitis is associated with a prothrombogenic phenotype, with P-selectin playing a major role in platelet recruitment. It has been suggested that probiotics may ameliorate colonic inflammation. We therefore investigated how treatment with Lactobacillus reuteri influenced P-selectin expression, leukocyte and platelet endothelial cell interactions, and colitis severity in DSS-treated rats. Rats were divided into the following four groups: nontreated, DSS treated (5% in drinking water for 9 days), L. reuteri, and L. reuteri and DSS treated. The rats were anesthetized with Inactin (120 mg/kg ip), and the dual radiolabeled monoclonal antibody technique was used to quantify P-selectin expression. Leukocyte-endothelial and platelet-endothelial cell interactions were studied in colonic venules with intravital microscopy. Colitis severity was assessed using a disease activity index. Disease activity index increased, as did the expression of P-selectin in the entire colon after DSS treatment, but both were reduced to control levels with L. reuteri pretreatment. The increased platelet- and leukocyte-endothelial cell interactions after DSS treatment were abolished by pretreatment with L. reuteri. L. reuteri protects against DSS-induced colitis in rats. The protection is associated with reduced P-selectin expression and a decrease in leukocyte- and platelet-endothelial cell interactions.
authors.affiliation: Department of Medical Cell Biology, Biomedical Center, Uppsala University, Box 571, Husarg. 3, 751 23 Uppsala, Sweden. Olof.Schreiber@mcb.uu.se
authors.forename: O
authors.initials: O
authors.lastname: Schreiber
authors.forename: J
authors.initials: J
authors.lastname: Petersson
authors.forename: M
authors.initials: M
authors.lastname: Phillipson
authors.forename: M
authors.initials: M
authors.lastname: Perry
authors.forename: S
authors.initials: S
authors.lastname: Roos
authors.forename: L
authors.initials: L
authors.lastname: Holm
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D003092:Colitis / Q000473:pathology / Q000517:prevention & control*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D052200:Limosilactobacillus reuteri; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D018715:Microscopy, Video; D019007:P-Selectin / Q000378:metabolism*; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019007:P-Selectin