title: Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase.
abstract: Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. In this study, recombinant B. canis Cu-Zn superoxide dismutase (SOD) was used as an antigen for the enzyme-linked immunosorbent assay (ELISA). The recombinant SOD showed a specific reaction with serum infected with B. canis in Western blotting and ELISA. These results suggest that ELISA using recombinant SOD is useful in screening for canine brucellosis.
authors.affiliation: Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: Ganbat
authors.initials: G
authors.lastname: Tsogtbaatar
authors.forename: Masato
authors.initials: M
authors.lastname: Tachibana
authors.forename: Kenta
authors.initials: K
authors.lastname: Watanabe
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Masahisa
authors.initials: M
authors.lastname: Watarai
mesh_terms: D000818:Animals; D041002:Brucella canis / Q000302:isolation & purification; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011994:Recombinant Proteins / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D013482:Superoxide Dismutase