Nocardioides basaltis sp. nov., isolated from black beach sand.
A novel Gram-positive, aerobic, short-rod-shaped bacterium, designated strain J112T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. The strain was found to be oxidase-negative and catalase-positive. Cells grew at 10-37 degrees C, at pH 5.5-8.0 and with 1-10 % NaCl. Growth occurred on marine agar but not on R2A or trypticase soy agar. A phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belongs to the radiation of the genus Nocardioides. Strain J112T shared the highest 16S rRNA gene sequence similarities with Nocardioides marinisabuli SBS-12T (99.2 %), Nocardioides terrigena DS-17T (97.3 %), Nocardioides kribbensis KCTC 19038T (97.1 %) and type strains of other Nocardioides species with validly published names (<97 %). The DNA-DNA hybridization values between strain J112T and the three most closely related strains were low enough to justify the assignment of this strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain J112T represents a novel species of the genus Nocardioides, for which the name Nocardioides basaltis sp. nov. is proposed. The type strain is J112T (=KCTC 19365T=JCM 14945T).
Kim
Kyoung-Ho
KH
Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
Roh
Seong Woon
SW
Chang
Ho-Won
HW
Nam
Young-Do
YD
Yoon
Jung-Hoon
JH
Jeon
Che Ok
CO
Oh
Hee-Mock
HM
Bae
Jin-Woo
JW
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D007723:Korea; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide