[Effect of Rapamycin and Cyclosporin A on the expression of TLR5 and Foxp3 in allorejection].
OBJECTIVE
To study the effect of Rapamycin (RAPA) and Cyclosporin A (CsA) on the expression of TLR5 and Foxp3 in allotransplantation model in vivo.


METHODS
The murine model of skin allotransplantation was established, and divided into three groups, injected with CsA 10 mg/(kg.d), RAPA 1.5 mg/(kg.d)) and normal saline respectively for 14 consecutive days. The grafts survival was observed daily. The expression of TLR5 and Foxp3 mRNA was detected by RT-PCR.


RESULTS
The survival time was obviously longer in RAPA and CsA treated groups than control group. The expression of TLR5 mRNA in allotransplantation treated with RAPA was higher than that in control group(P<0.05) and remained at a higher level than the other two groups until day 21. TLR5 showed the highest expression on day 10 among three groups, which was the top point of allorejection. Compared with CsA group, RAPA treatment in vivo caused the higher expression of Foxp3 mRNA(P<0.05), which remained at a high level after treatment stopped. However, in CsA group, Foxp3 mRNA expression increased on day 7 and 10, and then decreased significantly on day 14. TLR5 and Foxp3 expression was positively correlated among three groups. RAPA and CsA promoted the expression of TLR5 in T cells when treated with flagellin for 6 hours in vitro and RAPA has stronger effect.


CONCLUSIONS
RAPA and CsA can promote the expression of TLR5 and Foxp3 in allotransplantation model in vivo and flagellin enhanced this effect in vitro.
Chen
Fu-Qiang
FQ
Institute of Experimental Nuclear Medicine, Medical School of Shandong University, Jinan, China. cfqgen@163.com
Qu
Li-Li
LL
Zhang
Chao
C
Liang
Ting
T
Song
Jing
J
Xu
Jia
J
Hou
Gui-Hua
GH
D000818:Animals; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006084:Graft Rejection / Q000235:genetics*; D006085:Graft Survival / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020123:Sirolimus / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051198:Toll-Like Receptor 5 / Q000235:genetics*; D014184:Transplantation, Homologous
D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D051198:Toll-Like Receptor 5; D016572:Cyclosporine; D020123:Sirolimus