Draxin, a repulsive guidance protein for spinal cord and forebrain commissures.
Axon guidance proteins are critical for the correct wiring of the nervous system during development. Several axon guidance cues and their family members have been well characterized. More unidentified axon guidance cues are assumed to participate in the formation of the extremely complex nervous system. We identified a secreted protein, draxin, that shares no homology with known guidance cues. Draxin inhibited or repelled neurite outgrowth from dorsal spinal cord and cortical explants in vitro. Ectopically expressed draxin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo. draxin knockout mice showed defasciculation of spinal cord commissural axons and absence of all forebrain commissures. Thus, draxin is a previously unknown chemorepulsive axon guidance molecule required for the development of spinal cord and forebrain commissures.
Islam
Shahidul M
SM
Division of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Shinmyo
Yohei
Y
Okafuji
Tatsuya
T
Su
Yuhong
Y
Naser
Iftekhar Bin
IB
Ahmed
Giasuddin
G
Zhang
Sanbing
S
Chen
Sandy
S
Ohta
Kunimasa
K
Kiyonari
Hiroshi
H
Abe
Takaya
T
Tanaka
Satomi
S
Nishinakamura
Ryuichi
R
Terashima
Toshio
T
Kitamura
Toshio
T
Tanaka
Hideaki
H
D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000502:physiology*; D019556:COS Cells; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D003337:Corpus Callosum / Q000196:embryology / Q000378:metabolism; D018274:Electroporation; D020439:Growth Cones / Q000378:metabolism / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016501:Neurites / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis; D009457:Neuroglia / Q000378:metabolism; D016548:Prosencephalon / Q000002:abnormalities / Q000196:embryology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism; D046509:Tissue Culture Techniques
D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; C534831:draxin protein, mouse