title: Effect of porcine small intestinal submucosa on acute full-thickness wounds in dogs.
abstract: OBJECTIVE
  To evaluate the effects of porcine small intestinal submucosa (PSIS) on the healing of full-thickness wounds in dogs, specifically the appearance of granulation tissue, percent epithelialization and contraction, histologic variables of inflammation and repair, and aerobic culture results.
  
  
  METHODS
  Prospective, controlled, experimental study.
  
  
  METHODS
  Purpose-bred, female dogs (n=10).
  
  
  METHODS
  Wounds were created bilaterally on the trunk; 1 side as a control and 1 treated with PSIS. First appearance of granulation tissue was recorded. Total wound area, open wound area, and epithelialized area were measured at 21 time points-wound contraction and percent epithelialization were calculated. Aerobic cultures were taken at 4 time points and wound biopsies at 8. Histologic features were graded into an Acute Inflammation Score and Repair Score.
  
  
  RESULTS
  There was no difference in first appearance of granulation tissue between PSIS-treated and control wounds. Wound contraction was significantly faster in control wounds as was percent epithelialization after day 21. Histologic Acute Inflammation Scores were significantly higher in PSIS-treated wounds compared with control wounds on days 2 and 6. There were no differences in Histologic Repair Scores between PSIS-treated and control wounds or in aerobic culture results.
  
  
  CONCLUSIONS
  Wounds treated with PSIS contract more slowly, epithelialize less, and have more pronounced acute inflammation after implantation than control wounds.
  
  
  CONCLUSIONS
  Acute, full-thickness wounds in dogs do not benefit from treatment with PSIS.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Sandra P
authors.initials: SP
authors.lastname: Schallberger
authors.forename: Bryden J
authors.initials: BJ
authors.lastname: Stanley
authors.forename: Joe G
authors.initials: JG
authors.lastname: Hauptman
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Steficek
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D062109:Dermatologic Surgical Procedures; D004285:Dogs / Q000293:injuries* / Q000601:surgery*; D005260:Female; D006097:Granulation Tissue / Q000473:pathology / Q000502:physiology; D007413:Intestinal Mucosa / Q000502:physiology* / Q000637:transplantation; D011446:Prospective Studies; D011897:Random Allocation; D012867:Skin / Q000293:injuries*; D012879:Skin Physiological Phenomena; D013552:Swine; D013997:Time Factors; D017211:Treatment Failure; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000601:surgery / Q000628:therapy / Q000662:veterinary*