Jasmonates act with salicylic acid to confer basal thermotolerance in Arabidopsis thaliana.
* The cpr5-1 Arabidopsis thaliana mutant exhibits constitutive activation of salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) signalling pathways and displays enhanced tolerance of heat stress (HS). * cpr5-1 crossed with jar1-1 (a JA-amino acid synthetase) was compromised in basal thermotolerance, as were the mutants opr3 (mutated in OPDA reductase3) and coi1-1 (affected in an E3 ubiquitin ligase F-box; a key JA-signalling component). In addition, heating wild-type Arabidopsis led to the accumulation of a range of jasmonates: JA, 12-oxophytodienoic acid (OPDA) and a JA-isoleucine (JA-Ile) conjugate. Exogenous application of methyl jasmonate protected wild-type Arabidopsis from HS. * Ethylene was rapidly produced during HS, with levels being modulated by both JA and SA. By contrast, the ethylene mutant ein2-1 conferred greater thermotolerance. * These data suggest that JA acts with SA, conferring basal thermotolerance while ET may act to promote cell death.
Clarke
Shannon M
SM
Biochemistry Department, University of Otago, PO Box 56, Dunedin, New Zealand.|Present address: AgResearch Limited, Invermay Agricultural Centre, Puddle Alley, Private Bag 50034, Mosgiel, New Zealand.
Cristescu
Simona M
SM
Life Science Trace Gas Exchange Facility, Department of Molecular and Laser Physics, Radboud University, Heyendaalseweg 135, NL 6525 AJ Nijmegen. The Netherlands.
Miersch
Otto
O
Leibniz-Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/Saale, Germany.
Harren
Frans J M
FJM
Life Science Trace Gas Exchange Facility, Department of Molecular and Laser Physics, Radboud University, Heyendaalseweg 135, NL 6525 AJ Nijmegen. The Netherlands.
Wasternack
Claus
C
Leibniz-Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/Saale, Germany.
Mur
Luis A J
LAJ
Institute of Biological Sciences, University of Aberystwyth, SY23 3DA, UK.
D000222:Adaptation, Physiological / Q000187:drug effects*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D005030:Ethylenes / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018869:Heat-Shock Response / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009713:Nucleotidyltransferases / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013696:Temperature; D010453:Peptide Synthases
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D005231:Fatty Acids, Unsaturated; D008565:Membrane Proteins; D009713:Nucleotidyltransferases; D054883:Oxylipins; D012333:RNA, Messenger; D020156:Salicylic Acid; C112279:COI1 protein, Arabidopsis; C528008:CPR5 protein, Arabidopsis; C105496:NPR1 protein, Arabidopsis; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; C036216:ethylene; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases