Determination of suxamethonium in a pharmaceutical formulation by capillary electrophoresis with contactless conductivity detection (CE-C(4)D).
A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-C(4)D) was developed for the determination of suxamethonium (SUX) in a pharmaceutical formulation. A hydro-organic mixture, consisting of 100mM Tris-acetate buffer at pH 4.2 and acetonitrile (90:10, v/v), was selected as background electrolyte (BGE). The applied voltage was 30kV, and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50 microm and a total length of 64.5cm. Under these conditions, a complete separation between SUX, sodium ions and the main degradation products (choline) was achieved in less than 4min. The presence of acetonitrile in the BGE allowed a reduction of SUX adsorption on the capillary wall. The CE-C(4)D method was validated, and trueness values between 98.8% and 101.1% were obtained with repeatability and intermediate precision values of 0.7-1.3% and 1.2-1.6%, respectively. Therefore, this method was found appropriate for controlling pharmaceutical formulations containing suxamethonium and degradation products.
Nussbaumer
Susanne
S
Pharmacy, University Hospitals of Geneva , Geneva, Switzerland.
Fleury-Souverain
Sandrine
S
Rudaz
Serge
S
Bonnabry
Pascal
P
Veuthey
Jean-Luc
JL
D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000327:Adsorption; D002021:Buffers; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002794:Choline / Q000302:isolation & purification; D004553:Electric Conductivity; D004560:Electricity; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000302:isolation & purification; D015394:Molecular Structure; D009467:Neuromuscular Depolarizing Agents / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D012015:Reference Standards; D015203:Reproducibility of Results; D013390:Succinylcholine / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D013696:Temperature; D013997:Time Factors; D014325:Tromethamine / Q000737:chemistry
D000097:Acetonitriles; D002021:Buffers; D004573:Electrolytes; D007477:Ions; D009467:Neuromuscular Depolarizing Agents; D014325:Tromethamine; D013390:Succinylcholine; D002794:Choline; D019342:Acetic Acid; C032159:acetonitrile