Analysis of the fluorescence temporal point-spread function in a turbid medium and its application to optical imaging.
A time-domain optical method to evaluate the concentration (n), lifetime (tau), and depth (d) of a fluorescent inclusion is described by the complete analysis of the fluorescence temporal point-spread function (TPSF). The behavior of parameters in the fluorescence TPSF is explored, and we demonstrate the method with experimental data from a localized fluorescent inclusion in scattering media to recover images of n, tau, and d. The method has potential application for in vivo fluorescence imaging.
Han
Sung-Ho
SH
University of California at San Diego, Moores Cancer Center and Department of Radiology, 3855 Health Sciences Drive, 0819, La Jolla, California 92093-0819, USA. sunghohan@ucsd.edu
Farshchi-Heydari
Salman
S
Hall
David J
DJ
D000465:Algorithms; D003198:Computer Simulation; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D008956:Models, Chemical; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*