Selective gene amplification for high-throughput sequencing.
With tremendous progress in next generation sequencing technologies, it has become possible to perform genomic sequencing in a highly cost-effective manner. However, the human genome remains too complex to be routinely re-sequenced. Thus, selective gene amplification is often employed to target a subset of genomic regions (such as a set of disease-related genes) for deep sequencing analysis. Herein, we briefly review patents that can enrich target genomic regions of interest to harness the power of NextGen sequencing technology for basic and translational biomedical research.
Ni
Ting
T
Institute for Genome Sciences & Policy, Department of Cell Biology, Duke University Medical Center, 101 Science Drive, Durham, NC 27708, USA.
Wu
Han
H
Song
Shen
S
Jelley
Mark
M
Zhu
Jun
J
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005091:Exons; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010330:Patents as Topic; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013379:Substrate Specificity; D013698:Templates, Genetic