KIBRA Co-localizes with protein kinase Mzeta (PKMzeta) in the mouse hippocampus.
KIBRA is a WW domain-containing protein that can bind to protein kinase Czeta (PKCzeta). The SNP of the ninth intron of the KIBRA gene is associated with human episodic memory performance. Protein kinase Mzeta (PKMzeta), a brain-specific variant of PKCzeta, plays important roles in memory formation. Here we examined the interaction of KIBRA and PKMzeta in the adult mouse brain. Immunoprecipitation using newly-raised anti-KIBRA antibody revealed the interaction between KIBRA and PKMzeta in the brain. KIBRA was co-localized with PKMzeta in a single cultured neuron. Distribution analysis by immunohistochemistry and in situ hybridization indicated that KIBRA was highly localized with PKMzeta in the hippocampal CA1, CA3, and dentate gyrus. These results suggest that KIBRA functions in memory performance via interaction with PKMzeta.
Yoshihama
Yohei
Y
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo.
Hirai
Takaaki
T
Ohtsuka
Takashi
T
Chida
Kazuhiro
K
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D018891:Dentate Gyrus / Q000737:chemistry; D006624:Hippocampus / Q000737:chemistry*; D047908:Intracellular Signaling Peptides and Proteins; D008568:Memory; D051379:Mice; D009474:Neurons / Q000737:chemistry; D010750:Phosphoproteins; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D000099221:Protein Kinase C zeta
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D011493:Protein Kinase C; D000099221:Protein Kinase C zeta; C539495:Wwc1 protein, mouse