Curdlan microspheres. Synthesis, characterization and interaction with proteins (enzymes, vaccines).
Microparticles of curdlan, synthesized through crosslinking with epichlorohydrin in organic suspension media, were chemically modified with the aim of introducing strongly and/or weakly acidic anionic and palmitoyl hydrophobic groups. Microparticles of both curdlan and curdlan derivatives were physico-chemically characterized. Study of the interaction with enzymes, such as lysozyme, and vaccines, such as tetanus anatoxin, showed a co-operative protein retention effect, induced by electrostatic and hydrophobic forces. The results of the in vitro release studies on support-protein complexes recommend them as potential controlled release systems.
Mocanu
Georgeta
G
"Petru Poni" Institute of Macromolecular Chemistry, Iasi, Romania. gmocanu@icmpp.ro
Mihai
Doina
D
Moscovici
Misu
M
Picton
Luc
L
LeCerf
Didier
D
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004800:Enzymes, Immobilized / Q000378:metabolism; D007474:Ion Exchange; D007552:Isotonic Solutions; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013696:Temperature; D013744:Tetanus Toxin / Q000378:metabolism; D013997:Time Factors; D014612:Vaccines / Q000378:metabolism*; D047071:beta-Glucans / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D007552:Isotonic Solutions; D011506:Proteins; D013744:Tetanus Toxin; D014612:Vaccines; D047071:beta-Glucans; C038459:curdlan; D009113:Muramidase