Large-scale gene disruption using the UAU1 cassette.
Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.
Nobile
Clarissa J
CJ
Department of Microbiology Columbia University, NY, USA.
Mitchell
Aaron P
AP
D002176:Candida albicans / Q000235:genetics*; D004251:DNA Transposable Elements; D020868:Gene Silencing; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional / Q000379:methods*
D004251:DNA Transposable Elements