Receptor activator of nuclear factor-kappa B ligand induces osteoclast formation in RAW 264.7 macrophage cells via augmented production of macrophage-colony-stimulating factor.
RAW 264.7 macrophage cells differentiate into osteoclast-like cells in the presence of RANKL. Participation of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells was examined. TRAP-positive osteoclast-like cells appeared in RAW 264.7 cells cultured in the presence of RANKL. RANKL-induced osteoclast formation was markedly inhibited by anti-M-CSF antibody. RANKL augmented M-CSF mRNA expression and M-CSF production in RAW 264.7 cells. Further, anti-M-CSF antibody inhibited the expression of RANK, c-fms, c-fos and TRAP mRNA in RANKL-stimulated RAW 264.7 cells. However, anti-M-CSF antibody did not affect the expression of DC-STAMP in the stimulated cells. Therefore, RANKL was suggested to induce osteoclast formation in RAW 264.7 cells via augmented production of M-CSF. The putative role of M-CSF in RANKL-induced osteoclast formation of RAW 264.7 cells is discussed.
Islam
Shamima
S
Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
Hassan
Ferdaus
F
Tumurkhuu
Gantsetseg
G
Dagvadorj
Jargalsaikhan
J
Koide
Naoki
N
Naiki
Yoshikazu
Y
Yoshida
Tomoaki
T
Yokochi
Takashi
T
D000818:Animals; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D015870:Gene Expression; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000276:immunology; D053245:RANK Ligand / Q000276:immunology*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics / Q000276:immunology
D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D016173:Macrophage Colony-Stimulating Factor