title: Large-scale gene disruption using the UAU1 cassette.
abstract: Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.
authors.affiliation: Department of Microbiology Columbia University, NY, USA.
authors.forename: Clarissa J
authors.initials: CJ
authors.lastname: Nobile
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Mitchell
mesh_terms: D002176:Candida albicans / Q000235:genetics*; D004251:DNA Transposable Elements; D020868:Gene Silencing; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements