A novel real-time PCR method for KIR genotyping.
Genes encoding killer cell immunoglobulin-like receptors (KIRs) are variable among individuals. Sequence-specific primer-directed polymerase chain reaction (PCR) amplification (PCR-SSP) and sequence-specific oligonucleotide hybridization of the PCR-amplified products (PCR-SSO) are the methods currently used to characterize the diversity of KIR gene content. Both these methods include time-consuming post-PCR analyses. Here, we developed a real-time PCR method that identifies the presence or absence of 16 KIR genes during PCR and avoids post-PCR analyses. This method is specific, sensitive, shortens the turnaround time compared with the conventional PCR-SSP and PCR-SSO methods, and it can be easily adapted for automation.
Alves
L G T
LG
Genetic Division, Genoa Biotechnology SA, São Paulo, Brazil.
Rajalingam
R
R
Canavez
F
F
D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D054340:Receptors, KIR / Q000235:genetics*
D054340:Receptors, KIR