Free flow and capillary isoelectric focusing of bacteria from the tomatoes plant tissues.
The means of the preconcentration and preseparation of selected species or pathovars of bacteria directly from the plant tissue suspension by free flow isoelectric focusing are introduced here. After the focusing, the resulting fraction of microorganisms, native or dynamically modified by the non-ionogenic tenside on the basis of pyrenebutanoate, was separated by capillary isoelectric focusing and/or cultivated and positively identified by gas chromatographic analysis of fatty acid methyl esters. Simultaneously, capillary isoelectric focusing with UV and fluorometric detection was used for the rapid estimation of unknown isoelectric points of the examined plant pathogenic species of genus Clavibacter, Xanthomonas and Pseudomonas prior to the preconcentration and preseparation. The microorganisms were of different origin, native and/or dynamically modified by the non-ionogenic tenside.
Horká
Marie
M
Institute of Analytical Chemistry of the ASCR, v. v. i., Veverí 97, 602 00 Brno, Czech Republic. horka@iach.cz
Horký
Jaroslav
J
Matousková
Hana
H
Slais
Karel
K
D000192:Actinomycetales / Q000737:chemistry / Q000302:isolation & purification; D001419:Bacteria / Q000737:chemistry* / Q000302:isolation & purification*; D005453:Fluorescence; D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D018551:Solanum lycopersicum / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*; D011549:Pseudomonas / Q000737:chemistry / Q000302:isolation & purification; D014974:Xanthomonas / Q000737:chemistry / Q000302:isolation & purification