Production of nitric oxide and expression of inducible nitric oxide synthase in ovarian cystic tumors.
Tumor sections from nonneoplastic (n = 15), benign (n = 28), and malignant ovarian tumors (n = 20) were obtained from 63 women. Immunohistochemistry of the tumor sections demonstrated that inducible nitric oxide synthase (iNOS) expression was increased in ovarian cancer samples compared to nonneoplastic or benign tumor samples. Using the Griess method, nitric oxide (NO) metabolite levels were also found to be elevated in malignant tumor samples compared to benign tumor samples (P < .05). For stage I ovarian cancer, intracystic NO levels >80 microM were more frequent than NO levels <80 microM, and iNOS expression in well-differentiated carcinomas was greater than in moderately/poorly differentiated carcinomas (P < .05). These data suggest an important role for NO in ovarian carcinogenesis.
Nomelini
Rosekeila Simões
RS
Research Institute of Oncology (IPON), Discipline of Gynecology and Obstetrics, Federal University of Triângulo Mineiro, 38025-440 Uberaba, MG, Brazil.
de Abreu Ribeiro
Lívia Carolina
LC
Tavares-Murta
Beatriz Martins
BM
Adad
Sheila Jorge
SJ
Murta
Eddie Fernando Candido
EF
D000328:Adult; D005260:Female; D006801:Humans; D008875:Middle Aged; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D010048:Ovarian Cysts / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II