title: The effect of aberrant maspin expression on the invasive ability of pancreatic ductal adenocarcinoma cells.
abstract: Maspin is expressed aberrantly in pancreatic ductal adenocarcinoma (PDAC), and its function is still unknown. To explore the role of maspin in PDAC, we constructed wild-type maspin-expressing Panc-1 clones (Panc-1-maspin) by transfecting maspin cDNA into Panc-1, a PDAC-derived cell line that lacks maspin expression. As a control, mock-transfected clones (Panc-1-mock) were constructed using the same method. The invasive ability of the stable transfectants was evaluated with an in vitro invasion assay. The ability of Panc-1-maspin cells to migrate through a Matrigel-coated filter was significantly reduced compared to that of Panc-1-mock cells (p=0.012). In addition, we identified the c.1022A>G variant of maspin in human PDAC cells; however, this polymorphism was not involved in the clinical characteristics of PDAC nor did it alter the invasive ability of PDAC cells. The results of the present study indicate that maspin suppresses the invasive ability of PDAC cells.
authors.affiliation: Konkuk University Hospital, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Sung Noh
authors.initials: SN
authors.lastname: Hong
authors.forename: Jong Kyun
authors.initials: JK
authors.lastname: Lee
authors.forename: Won Hyoek
authors.initials: WH
authors.lastname: Choe
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Ha
authors.forename: Kyoungsook
authors.initials: K
authors.lastname: Park
authors.forename: In Kyung
authors.initials: IK
authors.lastname: Sung
authors.forename: Kyu Taek
authors.initials: KT
authors.lastname: Lee
authors.forename: Jae J
authors.initials: JJ
authors.lastname: Kim
authors.forename: Jong Chul
authors.initials: JC
authors.lastname: Rhee
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D015152:Blotting, Northern; D015153:Blotting, Western; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C085344:SERPIN-B5; D015843:Serpins