Phenotypic and genotypic diversity among strains of Aureobasidium pullulans in comparison with related species.
Intra-specific diversity of 200 Aureobasidium pullulans strains isolated from different sources and their relatives Kabatiella lini CBS 125.21 T and Hormonema prunorum CBS 933.72 T were studied by assessment of macromorphological, and physiological tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique (SDS-PAGE) of whole-cell proteins as well as enterobacterial repetitive intergenic consensus (ERIC)-, repetitive extragenic palindromic (REP)- and BOX-PCR techniques (collectively known as rep-PCR). Rep-PCR is an efficient procedure for discrimination of A. pullulans in terms of simplicity and rapidity. RFLP-PCR technique was applied for the identification of A. pullulans isolates and distinction from related species. This technique was insufficient for investigation of intra-specific diversity. The tested strains of A. pullulans could be divided into two groups based on their macromorphological, protein patterns obtained after SDS-PAGE as well as rep-PCR patterns. The first group of strains shared similar characteristics and was very different from the second one, designated as "complex group", consisting of strains with very little similarities within the group. Phenetic analysis of ERIC banding patterns failed to group the isolates on the basis of their substrate or geographical origin. Using 18S rDNA gene sequence analysis of selected isolates, three strains: HoHe3 km, A. pullulans DSM 62074 and H. prunorum CBS 933.72 T were distinguished from all other analysed members of genera Aureobasidium and Kabatiella.
Loncaric
Igor
I
Institute of Apiculture, Austrian Agency for Health and Food Safety (AGES), Spargelfeldstrasse 191, 1226 Vienna, Austria. igor.loncaric@ages.at
Oberlerchner
Josua T
JT
Heissenberger
Birgit
B
Moosbeckhofer
Rudolf
R
D000818:Animals; D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D012337:RNA, Ribosomal, 18S