[Jak/STAT signaling pathway of IL-11 in the protection of intestinal epithelial cells from neutron radiation].
OBJECTIVE
To explore the effect of IL-11 on the activation of Jak/STAT pathway and the expressions of Bax and Bcl-2 in the intestinal epithelial cells exposed to neutron radiation.


METHODS
The BALB/c mice and IEC-6, irradiated by 4 Gy neutron with or without IL-11 treatment, served as in vivo and in vitro model seperately. The changes of the intestines, activity of Jak1 and STAT3 and expressions of Bax and Bcl-2 were observed by HE staining, Western blot, EMSA, immunohistochemistry and image analysis.


RESULTS
(1)Mice exposed to neutron radiation showed severe intestinal damages and no obvious regeneration was seen. IL-11-treated mice had a larger number of cryptal epithelial cells and crypts. (2)Neutron radiation decreased the activities of Jak1 and STAT3, while IL-11 increased their activities. (3) Neutron radiation decreased the expression of Bax and didn't change the level of Bcl-2 in the murine intestine. IL-11 administration decreased the expression of Bax and increased that of Bcl-2.


CONCLUSIONS
The mechanism of the intestinal protection of IL-11 in neutron irradiation might be that IL-11 stimulation triggered activation of Jak/STAT pathway, downregulated the expression of Bax and upregulated the expression of Bcl-2.
Wang
Rui-Juan
RJ
Department of Experimental Pathology, Institute of Radiation Medicine, AMMS, Beijing, China. wangrjok@163.com
Peng
Rui-Yun
RY
Gao
Ya-Bing
YB
Chang
Gong-Min
GM
Xu
Xin-Ping
XP
Fu
Kai-Fei
KF
Luo
Qing-Liang
QL
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D007150:Immunohistochemistry; D017370:Interleukin-11 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology*; D053613:Janus Kinase 1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009502:Neutrons / Q000009:adverse effects*; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects
D017370:Interleukin-11; D050796:STAT3 Transcription Factor; D053613:Janus Kinase 1