[Changes in the DNA content in EGF-stimulated ciliate Tetrahymena pyriformis under effect of caffeine, KCl, and inhibitors of PLC and PKC].
It has been established that DNA content in ciliata Tetrahymena pyriformis changes under effect of the caffeine, and PLC and PKC inhibitors (U 73122 and chelerythrine accordingly). 10 mM caffeine stimulated DNA synthesis in these cells, 1 microM U 73122 and 25 microM chelerythrine, on the contrary, blockades the synthesis. 50 and 500 mM KCl, the agent which causes depolarization of plasma membrane stimulating Ca2+ entry into the cells, has no effect. The transduction of the proliferative signals in T. pyriformis cells has been proposed to be of Ca(2+)-dependent nature.
Shemarova
I V
IV
Selivanova
G V
GV
Vlasova
T D
TD
D000222:Adaptation, Physiological; D000818:Animals; D053119:Benzophenanthridines / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology*; D020013:Calcium Signaling; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects*; D016054:DNA, Protozoan / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004963:Estrenes / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011760:Pyrrolidinones / Q000494:pharmacology*; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
D053119:Benzophenanthridines; D003470:Culture Media; D016054:DNA, Protozoan; D004963:Estrenes; D010726:Phosphodiesterase Inhibitors; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D002110:Caffeine; D004815:Epidermal Growth Factor; D011189:Potassium Chloride; C016299:chelerythrine; D011493:Protein Kinase C; D010738:Type C Phospholipases