title: Indeterminate results of QuantiFERON TB-2G test performed in routine clinical practice.
abstract: The present authors assessed risk factors that can promote indeterminate results of QuantiFERON TB-2G (QFT-2G; Cellestis Ltd, Carnegie, Australia) tests. The subjects were 704 patients with suspected tuberculosis (TB) and latent TB infection between January 2005 and December 2007. The QFT-2G test and the tuberculin skin test (TST) were performed for all subjects. If the results of the QFT-2G test were indeterminate, the test was repeated within 1 month. In total, 72 (10.2%) patients showed indeterminate results on the QFT-2G test. Indeterminate results were due to positive control failure in 68 (88.9%) patients and negative control failure in four patients. The results of the TST were negative for 64 patients showing indeterminate results, the remaining eight patients showed a positive response to the TST. Indeterminate results were significantly associated with elderly and immunocompromised patients. Lymphocytopaenia and hypoalbuminaemia were significantly associated with indeterminate laboratory findings. When the QFT-2G test was repeated for all patients showing indeterminate results, 12 (16.7%) patients demonstrated determinate results on the subsequent test. Indeterminate results of the QuantiFERON TB-2G test under routine clinical practice are not infrequent. When scoring QuantiFERON TB-2G test results for elderly and immunocompromised patients, one must be careful because the possibility of obtaining determinate results may be low even if the test is repeated.
authors.affiliation: Division of Respiratory Diseases, Dept of Medicine, Kawasaki Medical School, Matsushima, Kurashiki, Japan. yoshihiro@med.kawasaki-m.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kobashi
authors.forename: T
authors.initials: T
authors.lastname: Sugiu
authors.forename: K
authors.initials: K
authors.lastname: Mouri
authors.forename: Y
authors.initials: Y
authors.lastname: Obase
authors.forename: N
authors.initials: N
authors.lastname: Miyashita
authors.forename: M
authors.initials: M
authors.lastname: Oka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016009:Chi-Square Distribution; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D012307:Risk Factors; D014374:Tuberculin Test / Q000379:methods* / Q000592:standards*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
chemical_list: D011933:Reagent Kits, Diagnostic