Morphological and microsatellite diversity associated with ecological factors in natural populations of Medicago laciniata Mill. (Fabaceae).
Genetic variability in 10 natural Tunisian populations of Medicago laciniata were analysed using 19 quantitative traits and 12 polymorphic microsatellite loci. A large degree of genetic variability within-populations and among-populations was detected for both quantitative characters and molecular markers. High genetic differentiation among populations for quantitative traits was seen, with Q(ST) = 0.47, and F(ST) = 0.47 for microsatellite markers. Several quantitative traits displayed no statistical difference in the levels of Q(ST) and F(ST). Further, significant correlations between quantitative traits and eco-geographical factors suggest that divergence in the traits among populations may track environmental differences. There was no significant correlation between genetic variability at quantitative traits and microsatellite markers within populations. The site-of-origin of eco-geographical factors explain between 18.13% and 23.40% of genetic variance among populations at quantitative traits and microsatellite markers, respectively. The environmental factors that most influence variation in measured traits among populations are assimilated phosphorus (P(2)0(5)) and mean annual rainfall, followed by climate and soil texture, altitude and organic matter. Significant associations between eco-geographical factors and gene diversity, He, were established in five microsatellite loci suggesting that these simple sequence repeats (SSRs) are not necessarily biologically neutral.
Badri
Mounawer
M
Laboratoire Interactions Légumineuses Microorganismes, Centre de Biotechnologie, Technopole de Borj-Cédria, B.P. 901, 2050 Hammam-Lif, Tunisia. mounawer_badri@yahoo.fr
Zitoun
Adel
A
Ilahi
Houcine
H
Huguet
Thierry
T
Aouani
Mohamed Elarbi
ME
D055669:Ecological and Environmental Phenomena; D008040:Genetic Linkage; D005819:Genetic Markers; D014644:Genetic Variation; D005843:Geography; D040582:Inheritance Patterns / Q000235:genetics; D029902:Medicago / Q000033:anatomy & histology* / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D010802:Phylogeny; D011157:Population Dynamics; D019655:Quantitative Trait, Heritable; D018709:Statistics, Nonparametric; D014416:Tunisia
D005819:Genetic Markers