Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices.
Herein we report on reliable reproducible quantification of protein analytes in human serum by fluorescence sandwich immunoassays in disposable PDMS microfluidic chips. The system requires 1,000 times less sample than typical clinical blood tests and is specifically shown to measure ferritin down to 250 pM in human serum. The in-built calibration method of spiking the serum with known concentrations of commercially available antigen avoids common sources of error and improves the reliability of the test results. The reported microfluidic system is an important new tool for fundamental scientific research, offering sensitive immunoassay measurements in small but complex biosamples. The system is also a further step towards comprehensive affordable "point-of-care" biomedical diagnostics.
Kartalov
Emil P
EP
Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089-9092, USA. kartalov@usc.edu
Lin
David H
DH
Lee
David T
DT
Anderson
William F
WF
Taylor
Clive R
CR
Scherer
Axel
A
D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results
D001798:Blood Proteins