title: Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.
abstract: Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.
authors.affiliation: Department of Neurosurgery, Teikyo University Chiba Medical Center, Anesaki, Ichihara City, Chiba, Japan. akirakun@med.teikyo-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuno
authors.forename: Johbu
authors.initials: J
authors.lastname: Itoh
authors.forename: Akiko
authors.initials: A
authors.lastname: Mizutani
authors.forename: Susumu
authors.initials: S
authors.lastname: Takekoshi
authors.forename: R Yoshiyuki
authors.initials: RY
authors.lastname: Osamura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Okinaga
authors.forename: Fuyuaki
authors.initials: F
authors.lastname: Ide
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyawaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uno
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Asano
authors.forename: Junichi
authors.initials: J
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakaguchi
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Sasaki
authors.forename: Mineko
authors.initials: M
authors.lastname: Murakami
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002457:Cell Extracts; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D013006:Growth Hormone / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014162:Transfection; D020692:rab3 GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002457:Cell Extracts; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C527701:enhanced cyan fluorescent protein; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D013006:Growth Hormone; C492132:Rab3b protein, rat; D020692:rab3 GTP-Binding Proteins