Identification and characterization of a novel protective antigen, Enolase of Streptococcus suis serotype 2.
Streptococcus suis serotype 2 (SS2) is a porcine and human pathogen with adhesive and invasive properties. The absence of suitable vaccine or virulent marker can be the bottleneck to control SS2 infection. In the present study, a novel immunogenic Enolase identified in the previous study was inducibly overexpressed in Escherichia coli, and the purified recombinant protein could elicit a significant humoral antibody response and confer efficient immunity against challenge with lethal dose of SS2 or SS7 infection in mouse model. The roles Enolase plays in pathogenicity of SS2 were also explored as reasons for which Enolase could be a protective antigen. The Enolase was an in vivo-induced antigen confirmed by the real-time PCR and could adhere to the Hep-2 cells by the indirect immunofluorescent assay and the inhibition assay. These suggested that Enolase could play important roles in pathogenicity and may serve as a novel vaccine candidate against SS2 infection.
Zhang
Anding
A
Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China.
Chen
Bo
B
Mu
Xiaofeng
X
Li
Ran
R
Zheng
Pei
P
Zhao
Yaxin
Y
Chen
Huanchun
H
Jin
Meilin
M
D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology; D012703:Serotyping; D017011:Streptococcus suis / Q000201:enzymology / Q000254:growth & development / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D010751:Phosphopyruvate Hydratase