Comparative proteomic analysis of human mesenchymal and embryonic stem cells: towards the definition of a mesenchymal stem cell proteomic signature.
Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue, we conducted a 2-DE gel analysis of mesenchymal stem cells isolated from bone marrow (BM), adipose tissue, synovial membrane and umbilical vein wall. We confirmed that BM and adipose tissue derived cells were very similar, which argue for their interchangeable use for cell therapy. We also compared human mesenchymal to embryonic stem cells and showed that umbilical vein wall stem cells, a neo-natal cell type, were closer to BM cells than to embryonic stem cells. Based on these proteomic data, we could propose a panel of proteins which were the basis for the definition of a mesenchymal stem cell proteomic signature.
Roche
Stephane
S
CNRS, Institut de Génétique Humaine, UPR1142, Montpellier, France.
Delorme
Bruno
B
Oostendorp
Robert A J
RA
Barbet
Romain
R
Caton
David
D
Noel
Daniele
D
Boumediene
Karim
K
Papadaki
Helen A
HA
Cousin
Beatrice
B
Crozet
Carole
C
Milhavet
Ollivier
O
Casteilla
Louis
L
Hatzfeld
Jacques
J
Jorgensen
Christian
C
Charbord
Pierre
P
Lehmann
Sylvain
S
D000273:Adipose Tissue / Q000166:cytology; D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D001853:Bone Marrow / Q000737:chemistry; D002478:Cells, Cultured; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D010641:Phenotype; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D013583:Synovial Membrane / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology
D015703:Antigens, CD; D011506:Proteins