Reversible control of exo- and endo-budding transitions in a photosensitive lipid membrane.
We have developed a method for the photomanipulation of lipid membrane morphology in which the shape of a vesicle can be switched by light through the use of a synthetic photosensitive amphiphile containing an azobenzene unit (KAON12). We prepared cell-sized liposomes from KAON12 and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and conducted real-time observations of vesicular transformation in the photosensitive liposome by phase-contrast microscopy. Budding transitions-either budding toward the centre of the liposome (endo-bud) or budding out of the liposome (exo-bud)-could be controlled by light. We discuss the mechanism of this transformation in terms of the change in the effective membrane surface area due to photoisomerization of the constituent molecules.
Ishii
Ken-ichi
K
School of Materials Science, Japan, Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Hamada
Tsutomu
T
Hatakeyama
Masaomi
M
Sugimoto
Ryoko
R
Nagasaki
Takeshi
T
Takagi
Masahiro
M
D004705:Endocytosis / Q000528:radiation effects; D005089:Exocytosis / Q000528:radiation effects; D007536:Isomerism; D008027:Light; D008081:Liposomes / Q000737:chemistry* / Q000528:radiation effects*; D008858:Microscopy, Phase-Contrast; D009997:Osmotic Pressure / Q000528:radiation effects; D010743:Phospholipids / Q000737:chemistry*
D008081:Liposomes; D010743:Phospholipids