Capillary zone electrophoresis method development for the analysis of Hippeastrum hybrid agglutinin samples.
A capillary zone electrophoresis (CZE) method was developed aiming the analysis of Hippeastrum hybrid agglutinin (HHA) samples. HHA is presently being tested as a vaginal microbicide to prevent HIV transmission. It acts by direct binding to mannose residues that are abundantly present on the HIV gp120 envelope and so interrupts the virus entry process. The final CZE method employs 50mM sodium tetraborate (pH 9.9) as background electrolyte. In this condition, a cluster of about 30 isoform peaks is obtained, with very repeatable patterns. RSDs in the order of 0.2% for the migration time and detection sensitivity in the order of 70microgml(-1) were achieved.
Carvalho
Alexandre Zatkovskis
AZ
Laboratory for Pharmaceutical Analysis, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, O&N2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
Dupas
Tine
T
Brouwers
Joachim
J
Hoogmartens
Jos
J
Augustijns
Patrick
P
Van Schepdael
Ann
A
D000373:Agglutinins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D019668:Liliaceae / Q000737:chemistry*; D008970:Molecular Weight; D015203:Reproducibility of Results
D000373:Agglutinins