title: Bacterial neuraminidase increases IL-8 production in lung epithelial cells via NF-kappaB-dependent pathway.
abstract: Bacterial neuraminidase, a sialic acid-degrading enzyme, is one of the virulent factors produced in pathogenic bacteria like as other bacterial components. However little is known about whether bacterial neuraminidase can initiate or modify a cellular response, such as cytokine production, in epithelial cells at infection and inflammation. We demonstrate here that bacterial neuraminidase, but not heat-inactivated neuraminidase, up-regulates expression of interleukin-8 (IL-8) mRNA and protein in lung epithelial A549 and NCI-H292 cells. We also show that bacterial neuraminidase significantly up-regulates IL-8 promoter activity as well as nuclear factor-kappaB (NF-kappaB) reporter activity. Moreover, inhibition of NF-kappaB signaling suppressed IL-8 mRNA expression induced by bacterial neuraminidase. Taken together, desialylation-induced IL-8 production in lung epithelial cells may play an important role in infection-associated inflammatory events.
authors.affiliation: Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto City, Kumamoto, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: Akinori
authors.initials: A
authors.lastname: Hisatsune
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Isohama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katsuki
mesh_terms: D045744:Cell Line, Tumor; D003016:Clostridium perfringens / Q000201:enzymology*; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009439:Neuraminidase / Q000276:immunology* / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects / Q000276:immunology*
chemical_list: D016209:Interleukin-8; D016328:NF-kappa B; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid