title: Curdlan microspheres. Synthesis, characterization and interaction with proteins (enzymes, vaccines).
abstract: Microparticles of curdlan, synthesized through crosslinking with epichlorohydrin in organic suspension media, were chemically modified with the aim of introducing strongly and/or weakly acidic anionic and palmitoyl hydrophobic groups. Microparticles of both curdlan and curdlan derivatives were physico-chemically characterized. Study of the interaction with enzymes, such as lysozyme, and vaccines, such as tetanus anatoxin, showed a co-operative protein retention effect, induced by electrostatic and hydrophobic forces. The results of the in vitro release studies on support-protein complexes recommend them as potential controlled release systems.
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Iasi, Romania. gmocanu@icmpp.ro
authors.forename: Georgeta
authors.initials: G
authors.lastname: Mocanu
authors.forename: Doina
authors.initials: D
authors.lastname: Mihai
authors.forename: Misu
authors.initials: M
authors.lastname: Moscovici
authors.forename: Luc
authors.initials: L
authors.lastname: Picton
authors.forename: Didier
authors.initials: D
authors.lastname: LeCerf
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004800:Enzymes, Immobilized / Q000378:metabolism; D007474:Ion Exchange; D007552:Isotonic Solutions; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013696:Temperature; D013744:Tetanus Toxin / Q000378:metabolism; D013997:Time Factors; D014612:Vaccines / Q000378:metabolism*; D047071:beta-Glucans / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D007552:Isotonic Solutions; D011506:Proteins; D013744:Tetanus Toxin; D014612:Vaccines; D047071:beta-Glucans; C038459:curdlan; D009113:Muramidase