Intranasal immunization of mice with VP2 DNA of human rotavirus a induces cellular and humoral immunity.
DNA vaccination using a plasmid encoding Human rotavirus A (HuRV-A) inner capsid VP2 was examined in a mouse model. BALB/c mice were immunized intranasally (i.n.) with a VP2 DNA vaccine that induced cellular and humoral immune response to HuRV-A. The increased levels of cytokines IFN-gamma and IL-4 and the production of anti-VP2 IgG antibodies were detected. In addition, splenocyte proliferation detected by MTT test was enhanced in the mice treated with the vaccine. These results may encourage the development of a HuRV-A DNA vaccine derived from the inner layer of viral capsid that can be administered i.n.
Pourasgari
F
F
Institute of Biochemistry and Biophysics, University of Tehran, Iran.
Ahmadian
S
S
Hassan
Z M
ZM
Mahdavi
M
M
Salmanian
A H
AH
Sarbolouki
M N
MN
Pothier
P
P
D000281:Administration, Intranasal; D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D056724:Immunity, Humoral; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology* / Q000821:virology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus; D019444:Vaccines, DNA; D014765:Viral Vaccines