Different strains of mice present distinct lung tissue mechanics and extracellular matrix composition in a model of chronic allergic asthma.
The impact of genetic factors on asthma is well recognized but poorly understood. We tested the hypothesis that different mouse strains present different lung tissue strip mechanics in a model of chronic allergic asthma and that these mechanical differences may be potentially related to changes of extracellular matrix composition and/or contractile elements in lung parenchyma. Oscillatory mechanics were analysed before and after acetylcholine (ACh) in C57BL/10, BALB/c, and A/J mice, subjected or not to ovalbumin sensitization and challenge. In controls, tissue elastance (E) and resistance (R), collagen and elastic fibres' content, and alpha-actin were higher in A/J compared to BALB/c mice, which, in turn, were more elevated than in C57BL/10. A similar response pattern was observed in ovalbumin-challenged animals irrespective of mouse strain. E and R augmented more in ovalbumin-challenged A/J [E: 22%, R: 18%] than C57BL/10 mice [E: 9.4%, R: 11%] after ACh In conclusion, lung parenchyma remodelled differently yielding distinct in vitro mechanics according to mouse strain.
Antunes
Mariana A
MA
Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, 21949-900, Rio de Janeiro, Brazil.
Abreu
Soraia C
SC
Damaceno-Rodrigues
Nilsa R
NR
Parra
Edwin R
ER
Capelozzi
Vera L
VL
Pinart
Mariona
M
Romero
Pablo V
PV
Silva
Patrícia M R
PM
Martins
Marco Aurélio
MA
Rocco
Patricia R M
PR
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000276:immunology*; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology*; D066298:In Vitro Techniques; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D015656:Respiratory Mechanics / Q000235:genetics* / Q000276:immunology; D013045:Species Specificity
D010047:Ovalbumin