The effect of N-acetylcysteine on biomarkers for radiation-induced oxidative damage in a rat model.
Our study aimed to investigate the potential radioprotective effects of N-acetylcysteine (NAC) by comparing its biochemical effects with those of WR-2721, as a representative of clinically used radioprotectors, in preventing oxidative damage caused by gamma irradiation (single dose, 6Gy) in normal rat tissue. The rats (n=40) were divided randomly and equally into 4 groups:Control (C), Radiation (R), R+NAC (received irradiation and 1,000 mg/kg NAC) and R+WR-2721 (received irradiation and 200 mg/kg WR-2721) rats. Liver tissues and blood samples were harvested and utilized for reduced glutathione (GSH), malondialdehyde (MDA) and myeloperoxidase (MPO) detection. Serum and tissue GSH levels of R rats decreased compared to those of other groups (p<0.01). Tissue MDA levels of R+NAC and R+WR-2721 rats decreased compared to R rats (p<0.01; p<0.05, respectively). Tissue MPO activities of R+NAC and R+WR-2721 rats were higher than those of R rats (p<0.001). Serum MPO levels of R+WR-2721 rats were lower than those of C rats and R rats (p<0.01, p<0.001, respectively). In conclusion, the study suggests that the radioprotective effect against radiation-induced oxidative damage of NAC may be similar to that of WR-2721.
Kilciksiz
Sevil
S
Department of Radiation Oncology, Faculty of Medicine, Gaziantep University, TR-27310 Gaziantep, Turkey. sevilkilciksiz@gmail.com
Demirel
Can
C
Erdal
Nurten
N
Gürgül
Serkan
S
Tamer
Lulufer
L
Ayaz
Lokman
L
Ors
Yasemin
Y
D000111:Acetylcysteine / Q000494:pharmacology*; D004999:Amifostine / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000097:blood; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood; D008099:Liver / Q000378:metabolism / Q000528:radiation effects; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D009195:Peroxidase / Q000097:blood; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000188:drug therapy* / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents; D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione; D004999:Amifostine; D000111:Acetylcysteine