title: Evaluation of a SRS2 sandwich commercial enzyme-linked immunosorbent assay for the detection of anti-Neospora caninum antibodies in bovine and canine sera.
abstract: Neospora caninum is a parasite responsible for abortion in cows and neuromuscular disease in dogs. Serology is the most widely used technique to evaluate the prevalence of N. caninum in different host populations. A sandwich enzyme-linked immunosorbent assay (ELISA) based on the use of an anti-SRS2 monoclonal antibody was evaluated against the indirect fluorescent antibody test for 100 canine sera and against a well-characterized ELISA for 102 bovine sera. In cattle sera, the relative sensitivity and relative specificity were 100%. In dog sera, the relative specificity and relative sensitivity were 94% and 86%, respectively. The kappa value was 1 for bovine sera and 0.77 for canine sera. The seroprevalence was 3.9% in bovine sera and 21-23% in canine sera. The SRS2 sandwich ELISA was considered a valuable tool in both species.
authors.affiliation: Parasitic Diseases Department, Faculty of Veterinary Medicine, Laboratory of Parasitology, University of Liege, Liege, Belgium. blosson@ulg.ac.be
authors.forename: Farida
authors.initials: F
authors.lastname: Ghalmi
authors.forename: Bernard
authors.initials: B
authors.lastname: China
authors.forename: Rachid
authors.initials: R
authors.lastname: Kaidi
authors.forename: Bertrand
authors.initials: B
authors.lastname: Losson
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000175:diagnosis; D003048:Coccidiosis / Q000097:blood / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D018688:Neospora / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan