Cystatins and cathepsin B during orthodontic tooth movement.
BACKGROUND
The lysosomal cysteine protease cathepsin B is known to play an important role in the resolution of organic matrix, a final step in bone resorption. Cystatins function as an inhibitor of cathepsin B. Determining the correlation between cathepsin B and cystatin levels in gingival crevicular fluid at various times might provide a better understanding of both the dynamics and the metabolic stages of orthodontic tooth movement.


METHODS
Human gingival crevicular fluid was collected at the distal sulcus from the canines of persons not in orthodontic treatment, in retention, and in retraction at various times (initial, 1 day, 1 week, and 1 month postretraction). Cathepsin B and its inhibitor, cystatin, were found with fluorometry.


RESULTS
The level of cathepsin B was varied in the retraction group; this was different from the retention and the nonorthodontic groups. Significant initial decreases after force application and subsequent increases by 1 month posttreatment were observed in the retraction group. The variations and differences among groups were negatively correlated with cystatin.


CONCLUSIONS
The balance between enzyme and inhibitor might reflect the clinical status of orthodontic tooth movement and provide valuable information for the assessment of recall intervals and retention procedures.
Rhee
Seung-Hoon
SH
Department of Pharmacy, Ajou University School of Medicine, Suwan, Korea.
Kang
Junghee
J
Nahm
Dong-Seok
DS
D002401:Cathepsin B / Q000032:analysis*; D003481:Cuspid / Q000473:pathology; D015891:Cystatins / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D016910:Orthodontic Brackets; D018704:Orthodontic Retainers; D009969:Orthodontic Wires; D013314:Stress, Mechanical; D013997:Time Factors; D014087:Tooth Movement Techniques / Q000295:instrumentation; D055815:Young Adult
D015891:Cystatins; D002401:Cathepsin B