title: New cell-based indicator assays for the detection of human cytomegalovirus infection and screening of inhibitors of viral immediate-early 2 protein activity.
abstract: OBJECTIVE
  Expression of early (E) genes of human cytomegalovirus (HCMV) is stimulated cooperatively by the activities of host cell transcription factors and the viral immediate-early 2 (IE2) protein. Taking advantage of the IE2-dependent inducibility of E gene promoters, in this study, we generated cell-based assays in which the expression of the enhanced green fluorescence protein (EGFP) reporter gene was driven by the UL54 or UL112/113 E promoters.
  
  
  RESULTS
  Cell clones derived from a stably transfected human cell line permissive to HCMV replication showed a specific and inducible dose- and time-dependent EGFP response to HCMV infection. The sensitivity of these indicator cells for detecting infectious particles of clinical isolates of HCMV was comparable to that of a conventional plaque assay. The HCMV-induced EGFP expression was completely prevented by treatment of indicator cells with fomivirsen, an antisense oligodeoxynucleotide designed to block IE2 expression, and this inhibitory activity was also observed when the IE2 protein alone was constitutively expressed in EGFP indicator cells.
  
  
  CONCLUSIONS
  The EGFP-based cell assays have proved to be a rapid, sensitive, quantitative and specific system for detection of HCMV and selection of antivirals.
  
  
  CONCLUSIONS
  These new cell-based assays can be exploited as functional assays to detect infectious HCMV particles, as well as to screen antiviral compounds that interfere with IE2 activity.
authors.affiliation: Department of Public Health and Microbiology, University of Torino, Torino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Luganini
authors.forename: P
authors.initials: P
authors.lastname: Caposio
authors.forename: M
authors.initials: M
authors.lastname: Mondini
authors.forename: S
authors.initials: S
authors.lastname: Landolfo
authors.forename: G
authors.initials: G
authors.lastname: Gribaudo
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000235:genetics / Q000821:virology*; D017781:Genes, Immediate-Early; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins