title: TBK1 does not play a role in the control of in vitro Burkholderia pseudomallei growth.
abstract: Burkholderia pseudomallei, the causative agent of melioidosis, is an important intracellular pathogen in tropical regions. TANK-binding kinase (TBK1), part of the pathway that induces transcription of Type I interferon genes, has been demonstrated to play an important role in controlling intracellular bacterial infections. To investigate the role of tbk1 in protecting against B. pseudomallei we developed tbk1-deficient cell lines by using shRNA for transient knockdown of the tbk1 gene in HeLa and RAW 264.7 cells. In tbk1-deficient RAW cells, the replication of invasive and non-invasive Escherichia coli was significantly increased at 48 h after infection compared with wild-type cells. The result was confirmed using Brucella melitensis in tbk1-deficient HeLa cells, which demonstrated a >1.5-2.0 log higher bacterial count at 6-48 h after infection compared to wild-type cells. By contrast, the growth of Burkholderia pseudomallei expressing either typical (A2) or atypical (G207) lipopolysaccharide was not significantly different between the tbk1-deficient and control cells. These results suggest that the tbk1 gene and its activation may be able to control invasive E. coli, non-invasive E. coli and B. melitensis growth but may not be able to control B. pseudomallei infection. The role of the tbk1 gene in proinflammatory cytokine induction and bacterial intracellular infection needs further investigation to identify mechanistic differences among the life cycles of various intracellular bacteria.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Pawana
authors.initials: P
authors.lastname: Panomket
authors.forename: Gary
authors.initials: G
authors.lastname: Splitter
authors.forename: Jerome
authors.initials: J
authors.lastname: Harms
authors.forename: Rasana W
authors.initials: RW
authors.lastname: Sermswan
authors.forename: Pleonchan
authors.initials: P
authors.lastname: Chedchotisakd
authors.forename: Surasakdi
authors.initials: S
authors.lastname: Wongratanacheewin
mesh_terms: D017347:Brucella melitensis / Q000254:growth & development; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000254:growth & development*; D002460:Cell Line; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques / Q000379:methods; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; C403191:TBK1 protein, human