Molecular cloning, polymorphism and association analyses of a novel differentially expressed porcine mRNA.
The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Meishan and Large White pigs. One novel mRNA that was differentially expressed was identified through semi-quantitative RT-PCR and the full-length cDNA sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the mRNA is not homologous to any of the known porcine genes. Sequence prediction analysis revealed that this mRNA is no-coding mRNA. Polymorphism analyses revealed that there was a C-T mutation on the position of 505 bp and PCR-HhaI-RFLP analyses revealed that Chinese indigenous pig breeds and exotic pig breeds displayed obvious genotype and allele frequency differences at this locus. Association analyses revealed that this polymorphic locus was significantly associated with the drip loss rate, water holding capacity, dressing percentage, rib numbers, lean meat percentage, estimated lean meat percentage, loin eye width and loin eye area (P < 0.05).
Liu
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GY
Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, 650201 Kunming, China. liuyg4567@163.com
Xiong
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YZ
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D056726:Genetic Association Studies; D016018:Least-Squares Analysis; D008297:Male; D008460:Meat; D018482:Muscle, Skeletal / Q000378:metabolism; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*
D012333:RNA, Messenger; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases