title: Tailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis.
abstract: Tailoring steps are often important for the activity of mature antibiotics. Here, we report that novel decarboxylated FR-008/candicidin derivatives were obtained from the P450 monooxygenase gene fscP mutant of Streptomyces sp. strain FR-008. The toxicity of decarboxylated FR-008/candicidin derivatives has been shown to be greatly reduced compared to that of wild-type FR-008/candicidin.
authors.affiliation: Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.forename: Xiangzhao
authors.initials: X
authors.lastname: Mao
authors.forename: Yaling
authors.initials: Y
authors.lastname: Shen
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jialiang
authors.initials: J
authors.lastname: Li
authors.forename: Lianrong
authors.initials: L
authors.lastname: Wang
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tao
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiufen
authors.initials: X
authors.lastname: Zhou
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000633:toxicity; D002174:Candicidin / Q000096:biosynthesis* / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects; D017353:Gene Deletion; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D011817:Rabbits; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002174:Candicidin; D006899:Mixed Function Oxygenases