title: Glucose determination using a re-usable enzyme-modified ion track membrane sensor.
abstract: A novel concept for a glucose biosensor based on the enzyme glucose oxidase covalently linked to nanopores of etched nuclear track membranes is presented. This device can be used to detect physiologically relevant glucose concentrations between 10 microM and 1 M. The sensitive catalytic sensor can be made re-usable due to the production of diffusible products from the oxidative biomolecular recognition event. We have demonstrated both the simplicity with which this sensor can be produced and the stability of the enzyme embedded in the nanopores. We strongly believe that the approach could be expanded for different enzymes and variable configurations of sensing and catalysis using membranes with nanopores for biocatalysis and enrichment of substrates and products. This is the first time that such devices are being used for biocatalytic transformations.
authors.affiliation: Department SF8, Lise Meitner Campus, Helmholtz Center for Materials and Energy, Berlin, Germany. fink@helmholtz-berlin.de
authors.forename: Dietmar
authors.initials: D
authors.lastname: Fink
authors.forename: Irina
authors.initials: I
authors.lastname: Klinkovich
authors.forename: Ohad
authors.initials: O
authors.lastname: Bukelman
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
authors.forename: Arik
authors.initials: A
authors.lastname: Kiv
authors.forename: David
authors.initials: D
authors.lastname: Fuks
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Fahrner
authors.forename: Lital
authors.initials: L
authors.lastname: Alfonta
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017743:Equipment Reuse; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D007477:Ions; D008567:Membranes, Artificial; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007477:Ions; D008567:Membranes, Artificial; D005949:Glucose Oxidase; D005947:Glucose