Synthesis and evaluation of a cyclic imine derivative conjugated to a fluorescent molecule for labeling of proteins.
A cyclic imine conjugated to a fluorescent dansyl group was synthesized and used for covalent labeling of proteins. The covalent attachment to proteins was confirmed by gel electrophoresis and mass analysis.
Guo
Hai-Ming
HM
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Minakawa
Maki
M
Ueno
Lynn
L
Tanaka
Fujie
F
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes; D007097:Imines / Q000138:chemical synthesis*; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis*; D014443:Tyrosine / Q000032:analysis / Q000737:chemistry
D005456:Fluorescent Dyes; D007097:Imines; D011506:Proteins; D014443:Tyrosine