[Microbial community structure of beer wastewater treatment plants analyzed by PCR-DGGE technique].
The microbial community structure of beer wastewater treatment plants with hydrolyze-acidification and SBR process was studied by PCR amplification and denaturing gradient gel electrophoresis (DGGE) based 16S rDNA. Activated sludge samples were collected from both hydrolyze-acidification tank and SBR tank at different depth and disposal period. The total DNA was extracted and the 16S rDNA was amplified by universal primer. The microbial community structure was analyzed by denaturing gradient gel electrophoresis, compared with the DGGE band pattern of the screened high efficient bacteria. The PCR products were cloned and sequenced to analyze the microbial community in phylogenic evolution. The DGGE pattern showed that the microbial community in hydrolyze-acidification tank changed with the variation of depth both in microbial structure and population, and at the depth of two-meter the microbial community was relatively rich, the dominant bands were notable. The microbial community in SBR tank at different depths and different disposal periods (precipitation period, inflow period, aeration period) were consistent respectively, but the dominant bands were different. Sequences of y2, 23, 25, 31, h5, 15 yielded high homogeneity with the strains uncultured Thermotogales sp., Comamonas sp. WT OTU1, Agrobacterium tumefaciens, Bacillus subtilis, Bdellovibrio bacteriovorus, Comamonas testosteroni from GenBank. The 16S rDNA sequences of the dominant bands were not completely same with that of the high efficient bacteria, which indicated that the strains screened were not the dominant strains in actual process.
Zhao
Ji-Hong
JH
School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China. zhaojihong@haut.edu.cn
He
Shu-Ying
SY
Li
Ji-Xiang
JX
Liu
Yong-De
YD
Lou
Yan
Y
D001515:Beer; D019149:Bioreactors / Q000382:microbiology*; D020585:Comamonas / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012231:Rhizobium / Q000235:genetics / Q000302:isolation & purification; D020124:Thermotoga maritima / Q000235:genetics / Q000302:isolation & purification*; D014865:Waste Disposal, Fluid / Q000379:methods*