Mapping metals in Parkinson's and normal brain using rapid-scanning x-ray fluorescence.
Rapid-scanning x-ray fluorescence (RS-XRF) is a synchrotron technology that maps multiple metals in tissues by employing unique hardware and software to increase scanning speed. RS-XRF was validated by mapping and quantifying iron, zinc and copper in brain slices from Parkinson's disease (PD) and unaffected subjects. Regions and structures in the brain were readily identified by their metal complement and each metal had a unique distribution. Many zinc-rich brain regions were low in iron and vice versa. The location and amount of iron in brain regions known to be affected in PD agreed with analyses using other methods. Sample preparation is simple and standard formalin-fixed autopsy slices are suitable. RS-XRF can simultaneously and non-destructively map and quantify multiple metals and holds great promise to reveal metal pathologies associated with PD and other neurodegenerative diseases as well as diseases of metal metabolism.
Popescu
Bogdan F Gh
BF
Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan SK Canada.
George
Martin J
MJ
Bergmann
Uwe
U
Garachtchenko
Alex V
AV
Kelly
Michael E
ME
McCrea
Richard P E
RP
Lüning
Katharina
K
Devon
Richard M
RM
George
Graham N
GN
Hanson
Akela D
AD
Harder
Sheri M
SM
Chapman
L Dean
LD
Pickering
Ingrid J
IJ
Nichol
Helen
H
D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D001921:Brain / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008670:Metals / Q000032:analysis*; D010300:Parkinson Disease / Q000378:metabolism*; D011857:Radiographic Image Interpretation, Computer-Assisted / Q000379:methods*; D012016:Reference Values; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D014018:Tissue Distribution
D008670:Metals