[Concentration of selected cytokines in serum of patients with acne rosacea].
BACKGROUND
Rosacea is a common chronic disorder, mainly affects women in their thirties or fourties. As etiology, inflammation is taken into consideration.


OBJECTIVE
To assess the concentrations of proinflammatory cytokines: IL-6, IL-18, TNF-alpha and CRP protein.


METHODS
the study was performed in 60 patients (45 women and 15 men) with rosacea, median age 51 years (range 28-82 years). Twenty five healthy volunteers, sex and age matched, served as a control group. LEVELS OF SELECTED PARAMETERS OF INFLAMMATION: IL-6, IL-18 were detected by ELISA (Bender MedSystems GmbH, Vienna Austria), level of TNF alpha was detected by an immunoenzymatic, chemiluminescent, sequential test IMMULITE TNF-alpha, while detection of CRP was made using an immunonephelometric method using N High Sensitivity CRP (Dade Behring, Inc.).


RESULTS
The concentration of IL-6 in the group of patients was statistically significantly lower than in the control group, 0.9 pg/ml and 1.8 pg/ ml respectively, p < 0.001. In the group of the patients with PPR, obtained result (0.9 pg/ml) did not differ from the group with ETR (1.15 pg/ml), p > 0.05. The concentration of IL-18 in the patients was 163.5 pg/ml and it was statistically significantly lower than median level of this protein in the control group (16.2 pg/ml), p < 0.01. The concentration of TNF alpha in the group of patients was 5.79 pg/ml and was higher than the level in the control group -4.73 pg/ml; nevertheless there was no statistically significant correlations, p > 0.05. Median concentration of TNF alpha in the patients with PPR was 4.975 pg/ml and in the patients with ETR 6.73 pg/ml, p > 0.05. Median concentration of CRP in the group with PPR was 0.085 mg/dl and with ETR 0.14 mg/dl.


CONCLUSIONS
Disturbed levels of IL-6 and IL-18, independent of clinical type of rosacea, confirm their role in the development of an inflammatory state in the course of the disease. This kind of correlation was not present for TNF alpha and CRP protein.
Salamon
Małgorzata
M
Katedra i Klinika Dermatologii i Wenerologii, Uniwersytetu Medycznego w Łodzi.
Sysa-Jedrzejowska
Anna
A
Lukamowicz
Jolanta
J
Lukamowicz
Magdalena
M
Swiatkowska
Ewa
E
Wozniacka
Anna
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002097:C-Reactive Protein / Q000378:metabolism; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D012393:Rosacea / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
D016207:Cytokines; D020382:Interleukin-18; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein