Fucoidan inhibits parainfluenza virus type 2 infection to LLCMK2 cells.
The effects of fucoidan and L-fucose, a fundamental major component of fucoidan, on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK(2) cells were investigated. Fucoidan inhibited cell fusion and hemadsorption, but L-fucose only partly inhibited both. Virus RNA was not detected in the hPIV-2 infected cells cultured with fucoidan. However, L-fucose did not inhibit virus RNA synthesis. Indirect immunofluorescence study showed that virus protein synthesis was inhibited by fucoidan, but not by L-fucose. Furthermore, using a recombinant, green fluorescence protein-expressing hPIV-2, it was found that virus entry was inhibited by fucoidan, but not by L-fucose. These results suggested that fucoidan inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented infection, indicating that the sulfated polysaccharide form was important for the inhibition by fucoidan.
Taoda
Naomi
N
Department of Microbiology, Suzuka University of Medical Science, Suzuka, Mie, Japan.
Shinji
Eri
E
Nishii
Kumiko
K
Nishioka
Saori
S
Yonezawa
Yukari
Y
Uematsu
Jun
J
Hattori
Emi
E
Yamamoto
Hidetaka
H
Kawano
Mitsuo
M
Tsurudome
Masato
M
O'Brien
Myles
M
Yamashita
Tetsuro
T
Komada
Hiroshi
H
D002460:Cell Line; D002648:Child; D005643:Fucose / Q000378:metabolism; D006801:Humans; D010223:Parainfluenza Virus 2, Human / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D019351:Rubulavirus Infections / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
D011134:Polysaccharides; D014764:Viral Proteins; D005643:Fucose; C007789:fucoidan