[Binding of human ribosomal protein p40 and its truncated mutants to the small ribosomal subunit].
Ribosomal protein SA (rpSA) or p40 is a structural element of the small subunit of the eukaryotic ribosome. N-terminal and central parts of the protein are homologous to prokaryotic rpS2 whereas its C-terminal part is eukaryote specific. In this study we showed that samples of 40S ribosomal subunits isolated from full-term human placenta are variably deficient in the rpSA content. To reveal rpSA ability to bind to human 40S ribosomal subunits, recombinant rpSA and its mutant forms with N- and C-terminal deletions have been synthesized. It was shown that both full-size and truncated from the N-terminus proteins were able to bind to the 40S subunits whereas the mutant truncated from C-terminus was not.
Kosinova
O A
OA
Malygin
A A
AA
Babaĭlova
E S
ES
Karpova
G G
GG
D000595:Amino Acid Sequence; D005260:Female; D006801:Humans; D010920:Placenta / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D017474:Receptors, Laminin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
C510624:RPSA protein, human; D017474:Receptors, Laminin; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C030004:ribosomal protein S2