Immobilized metal affinity electrophoresis: a novel method of capturing phosphoproteins by electrophoresis.
An immobilized metal affinity electrophoresis (IMAEP) method is described here. In this method, metal ions are immobilized in a native polyacrylamide gel to capture phosphoproteins. The capture of phosphoproteins by IMAEP is demonstrated with immobilized metals like iron, aluminum, manganese, or titanium. In the case studies, phosphoproteins alpha-casein, beta-casein, and phosvitin are successfully extracted from a protein mixture by IMAEP.
Lee
Bao-Shiang
BS
Protein Research Laboratory, Research Resources Center, University of Illinois at Chicago, Chicago, IL 60612-3748, USA. boblee@uic.edu
Lasanthi
G D
GD
Jayathilaka
P
P
Huang
Jin-Sheng
JS
Gupta
Shalini
S
D000345:Affinity Labels; D001709:Biotechnology; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008670:Metals; D010750:Phosphoproteins / Q000302:isolation & purification*; D010774:Phosvitin / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000345:Affinity Labels; D002364:Caseins; D008670:Metals; D010750:Phosphoproteins; D010774:Phosvitin