[Sodium butyrate do not induce the program of premature senescence in transformants with JNK1,2 knockout].
We studied the role of JNK1,2 stress-kinases in the regulation of premature senescence program, stimulated by the inhibitor of histone deacetylase, sodium butyrate (NaB). It was found, that the transformants EIA + cHa-ras selected from embryonic mouse fibroblasts with knockout jnk1,2 stress-kinase genes did not block the cell cycle after sodium butyrate treatment. The data on the cell cycle distribution and cell growth curves showed that even long term (during five days) NaB influence did not suppress proliferation. We did not also reveal any cellular hypertrophy and increase in SA-beta-galactosidase activity after NaB treatment. The data presented suggest that JNK stress-kinases are involved in sodium butyrate-induced senescence in E1A + cHa-Ras mouse transformants, and they are indicative of that JNK1,2 have tumor suppressor properties.
Zubova
S G
SG
Bykova
T V
TV
Zubova
Iu G
IuG
Romanov
V S
VS
Aksenov
N D
ND
Pospelov
V A
VA
Pospelova
T V
TV
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000502:physiology*; D050881:E1A-Associated p300 Protein / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects; D011905:Genes, ras / Q000235:genetics; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D048055:Mitogen-Activated Protein Kinase 8 / Q000235:genetics / Q000502:physiology*; D048056:Mitogen-Activated Protein Kinase 9 / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D002087:Butyrates; D056572:Histone Deacetylase Inhibitors; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse; D048056:Mitogen-Activated Protein Kinase 9; D048055:Mitogen-Activated Protein Kinase 8; D001616:beta-Galactosidase; D006655:Histone Deacetylases