Dietary supplementation with S-adenosyl methionine was associated with protracted reduction of seizures in a line of transgenic mice.
Transgenic mice, although useful for analyses of gene function, can present unanticipated phenotypic manifestations, including behavioral problems, that may not be directly associated with the gene of interest but rather due to the complex interplay inherent in genomes. These unexpected events can present unique insight into gene function, leading to an advantage in some situations, yet in others can confound interpretation and compromise usefulness of the transgenic line. Here we document that short-term supplementation with S-adenosyl methionine (SAM)--a nutriceutical known to regulate neurotransmitter levels, improve working memory, and reduce aggression--reduced handling- and startling-induced seizures that otherwise precluded behavioral analyses in a transgenic line. This effect lasted for at least 1 mo after withdrawal of SAM and allowed mice to be used in standard maze analyses. These findings suggest that short-term administration of a neurotropic nutriceutical may provide a functional rescue for behavioral studies in an otherwise intractable transgenic mouse line as well as improve the welfare of similar lines.
Perry
Sheryl
S
Center for Cellular Neurobiology and Neurodegeneration Research, University of Massachusetts Lowell, Lowell, Massachusetts, USA.
Levasseur
James
J
Chan
Amy
A
Shea
Thomas B
TB
D000544:Alzheimer Disease / Q000235:genetics / Q000503:physiopathology / Q000523:psychology; D000818:Animals; D053327:Apolipoprotein E4 / Q000235:genetics; D001522:Behavior, Animal / Q000187:drug effects; D019587:Dietary Supplements; D004195:Disease Models, Animal; D006801:Humans; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000235:genetics; D012436:S-Adenosylmethionine / Q000008:administration & dosage*; D012640:Seizures / Q000517:prevention & control*
D053327:Apolipoprotein E4; D011994:Recombinant Proteins; D012436:S-Adenosylmethionine