title: Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectors.
abstract: Gene delivery to stem cells holds great potential for tissue regeneration and delivery of therapeutic proteins. The major barrier is the lack of safe and efficient delivery methods. Here, we report enhanced gene delivery systems for human stem cells using biodegradable polymeric vectors. A library of poly (beta-amino esters) end-modified derivatives was developed and optimized for high transfection efficiency and low cytotoxicity for three human stem cell lines including human mesenchymal stem cells (hMSCs), human adipose-derived stem cells (hADSCs) and human embryonic stem cell-derived cells (hESCds). In the presence of 10% serum, leading end-modified C32 polymeric vectors exhibited significantly high transfection efficiency in hMSCs (27+/-2%), hADSCs (24+/-3%) and hESCds (56+/-11%), with high cell viability (87-97%) achieved in all cell types. Our results show that poly(beta-amino esters) as a class, and end-modified versions of C32 in particular, are efficient polymeric vectors for gene delivery to both adult and embryonic-derived stem cells.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: J J
authors.initials: JJ
authors.lastname: Green
authors.forename: T
authors.initials: T
authors.lastname: Dinio
authors.forename: L
authors.initials: L
authors.lastname: Keung
authors.forename: S-W
authors.initials: SW
authors.lastname: Cho
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.forename: R
authors.initials: R
authors.lastname: Langer
authors.forename: D G
authors.initials: DG
authors.lastname: Anderson
mesh_terms: D020341:Absorbable Implants; D002470:Cell Survival; D053595:Embryonic Stem Cells / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D053758:Nanoparticles; D010316:Particle Size; D013234:Stem Cells / Q000378:metabolism*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A