An STE12 gene identified in the mycorrhizal fungus Glomus intraradices restores infectivity of a hemibiotrophic plant pathogen.
Mechanisms of root penetration by arbuscular mycorrhizal (AM) fungi are unknown and investigations are hampered by the lack of transformation systems for these unculturable obligate biotrophs. Early steps of host infection by hemibiotrophic fungal phytopathogens, sharing common features with those of AM fungal colonization, depend on the transcription factor STE12. Using degenerated primers and rapid amplification of cDNA ends, we isolated the full-length cDNA of an STE12-like gene, GintSTE, from Glomus intraradices and profiled GintSTE expression by real-time and in situ RT-PCR. GintSTE activity and function were investigated by heterologous complementation of a yeast ste12Delta mutant and a Colletotrichum lindemuthianum clste12Delta mutant. * Sequence data indicate that GintSTE is similar to STE12 from hemibiotrophic plant pathogens, especially Colletotrichum spp. Introduction of GintSTE into a noninvasive mutant of C. lindemuthianum restored fungal infectivity of plant tissues. GintSTE expression was specifically localized in extraradicular fungal structures and was up-regulated when G. intraradices penetrated roots of wild-type Medicago truncatula as compared with an incompatible mutant. Results suggest a possible role for GintSTE in early steps of root penetration by AM fungi, and that pathogenic and symbiotic fungi may share common regulatory mechanisms for invasion of plant tissues.
Tollot
Marie
M
UMR INRA 1088/CNRS 5184/Université de Bourgogne, Plante-Microbe-Environnement, 17 Rue Sully - BP 86510 - 21065 Dijon Cedex, France.
Wong Sak Hoi
Joanne
J
van Tuinen
Diederik
D
Arnould
Christine
C
Chatagnier
Odile
O
Dumas
Bernard
B
Gianinazzi-Pearson
Vivienne
V
Seddas
Pascale M A
PM
D000595:Amino Acid Sequence; D020231:Colletotrichum / Q000235:genetics / Q000472:pathogenicity*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D018525:Germination / Q000235:genetics; D055137:Glomeromycota / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D046913:Medicago truncatula / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D038821:Mycorrhizae / Q000235:genetics*; D027805:Phaseolus / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013172:Spores, Fungal / Q000235:genetics
D005656:Fungal Proteins; D012333:RNA, Messenger