title: SCARF: maximizing next-generation EST assemblies for evolutionary and population genomic analyses.
abstract: CONCLUSIONS
  Scaffolded and Corrected Assembly of Roche 454 (SCARF) is a next-generation sequence assembly tool for evolutionary genomics that is designed especially for assembling 454 EST sequences against high-quality reference sequences from related species. The program was created to knit together 454 contigs that do not assemble during traditional de novo assembly, using a reference sequence library to orient the 454 sequences.
  
  
  BACKGROUND
  SCARF is freely available at http://msbarker.com/software.htm, and is released under the open source GPLv3 license (http://www.opensource.org/licenses/gpl-3.0.html.
authors.affiliation: Botany Department, University of British Columbia, Vancouver, B.C. V6T 1Z4, Canada. msbarker@indiana.edu
authors.forename: Michael S
authors.initials: MS
authors.lastname: Barker
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Dlugosch
authors.forename: A Chaitanya C
authors.initials: AC
authors.lastname: Reddy
authors.forename: Sarah N
authors.initials: SN
authors.lastname: Amyotte
authors.forename: Loren H
authors.initials: LH
authors.lastname: Rieseberg
mesh_terms: D031179:Centaurea / Q000235:genetics; D030541:Databases, Genetic; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D016678:Genome; D023281:Genomics / Q000379:methods*; D012984:Software