[Pre-L-methionine feeding strategy for S-adenosyl-L-methionine fermentative production].
The yield of S-adenosyl-L-methionine (SAM) on high-cell-density fermentation by saccharomyces cerevisiae is mostly affected by the feeding strategy of pre-L-methionine. The mutant strain SAM0801 that could accumulate more SAM was used in this study. Six high-cell-density fermentation experiments in 5 L fermentor were investigated to get the optimal feeding time and amount of L-methionine. The results showed that when 40 g L-methionine was added in the fermentor after 30 h fermentation, a dry cell weight of 100 g/L was achieved. Under this condition, after 58 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 168 g/L and 14.48 g/L respectively.
Wang
Jiepeng
J
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Tan
Tianwei
T
D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D008715:Methionine / Q000032:analysis / Q000378:metabolism*; D009154:Mutation; D012436:S-Adenosylmethionine / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D012436:S-Adenosylmethionine; D008715:Methionine