title: Sperm quality improvement after date seed oil in vitro supplementation in spontaneous and induced oxidative stress.
abstract: In vitro supplementation with date seed oil (DSO) can protect spermatozoa against hydrogen peroxide (H2O2)-mediated damage and can improve sperm function, possibly owing to antioxidant properties. We tested the antioxidant effects of DSO on human sperm motility, sperm viability, reacted acrosome and lipid peroxidation assessed in vitro after H2O2-mediated oxidative damage in spermatozoa. Sixteen patients (mean age: 35 years; range: 25-45 years) referred to the Histology-Embryology Laboratory of the Medicine Faculty of Sfax for semen analysis after 12-24 months of sexual intercourse without conception were selected. After spermiogram, sperm selection by two-interface discontinuous Sill Select gradient was performed, and selected spermatozoa were used in four experimental assays: control; incubation with 100 microm H2O2; incubation with 0.1% DSO; and co-incubation with 0.1% DSO and 100 microm H2O2. Motility and viability were determined using World Health Organization criteria. Acrosome reaction and lipid peroxidation were assessed by staining with fluorescein isothiocyanate-Pisum sativum and spectrophotometric measurement of malondialdehyde, respectively. Results showed that incubation with H2O2 alone led to a significant increase in lipid peroxidation (57.83%, P<0.05) associated with a significant decrease in sperm motility, sperm viability (after 30 min and 24 h) and percentage of reacted acrosome (P<0.05). Date seed oil improved sperm motility after 24 h of incubation (P<0.05) and protected spermatozoa against the deleterious effects of H2O2 on motility, viability, acrosome reaction and lipid peroxidation. We conclude that supplementation with DSO may have a function in antioxidant protection against male infertility.
authors.affiliation: Human Pathologies and Oxidative Stress Unit, Superior Institute of Biotechnology of Sfax, Laboratory of Histology, Embryology and Reproductive Biology, Medicine Faculty of Sfax, Sfax 3028, Tunisia. fatma_isbs@yahoo.fr
authors.forename: Ben A
authors.initials: BA
authors.lastname: Fatma
authors.forename: Chakroun F
authors.initials: CF
authors.lastname: Nozha
authors.forename: Dammak
authors.initials: D
authors.lastname: Ines
authors.forename: Attia
authors.initials: A
authors.lastname: Hamadi
authors.forename: Hentati
authors.initials: H
authors.lastname: Basma
authors.forename: Ammar K
authors.initials: AK
authors.lastname: Leila
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000328:Adult; D028023:Arecaceae; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology*; D012639:Seeds; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D016877:Oxidants; D010938:Plant Oils; D006861:Hydrogen Peroxide