title: Reflectometric interference spectroscopy.
abstract: Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance (SPR). The advantages of direct optical detection depend on a sophisticated surface chemistry resulting in negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Tuebingen, Tuebingen, Germany.
authors.forename: Guenther
authors.initials: G
authors.lastname: Proll
authors.forename: Goran
authors.initials: G
authors.lastname: Markovic
authors.forename: Lutz
authors.initials: L
authors.lastname: Steinle
authors.forename: Guenter
authors.initials: G
authors.lastname: Gauglitz
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods