title: A chromatic explosion: the development and future of multiparameter flow cytometry.
abstract: Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.
authors.affiliation: ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3015, USA. pchattop@mail.nih.gov
authors.forename: Pratip K
authors.initials: PK
authors.lastname: Chattopadhyay
authors.forename: Carl-Magnus
authors.initials: CM
authors.lastname: Hogerkorp
authors.forename: Mario
authors.initials: M
authors.lastname: Roederer
mesh_terms: D000818:Animals; D004396:Coloring Agents; D004867:Equipment Design; D005434:Flow Cytometry / Q000379:methods* / Q000639:trends; D005544:Forecasting; D006801:Humans; D007154:Immune System Diseases / Q000175:diagnosis; D013223:Statistics as Topic
chemical_list: D004396:Coloring Agents