title: Optimization of separation and online sample concentration of N,N-dimethyltryptamine and related compounds using MEKC.
abstract: The optimal separation conditions and online sample concentration for N,N-dimethyltryptamine (DMT) and related compounds, including alpha-methyltryptamine (AMT), 5-methoxy-AMT (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DiPT), 5-methoxy-DMT (5-MeO-DMT), and 5-methoxy-N,N-DiPT (5-MeO-DiPT), using micellar EKC (MEKC) with UV-absorbance detection are described. The LODs (S/N = 3) for MEKC ranged from 1.0 1.8 microg/mL. Use of online sample concentration methods, including sweeping-MEKC and cation-selective exhaustive injection-sweep-MEKC (CSEI-sweep-MEKC) improved the LODs to 2.2 8.0 ng/mL and 1.3 2.7 ng/mL, respectively. In addition, the order of migration of the nine tryptamines was investigated. A urine sample, obtained by spiking urine collected from a human volunteer with DMT, was also successfully examined.
authors.affiliation: Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.
authors.forename: Man-Juing
authors.initials: MJ
authors.lastname: Wang
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Tsai
authors.forename: Wei-Ya
authors.initials: WY
authors.lastname: Hsu
authors.forename: Ju-Tsung
authors.initials: JT
authors.lastname: Liu
authors.forename: Cheng-Huang
authors.initials: CH
authors.lastname: Lin
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary; D015394:Molecular Structure; D004130:N,N-Dimethyltryptamine / Q000737:chemistry* / Q000302:isolation & purification*; D009862:Online Systems; D013057:Spectrum Analysis
chemical_list: D004130:N,N-Dimethyltryptamine