[Utilizing of protein splicing for human growth hormone purification].
One of the most important problems in biotechnology today is development of simple and cheap techniques for protein purification. This is why inteins and protein splicing phenomena are of a great interest for protein purification. Modification of inteins by affinity tags permits to use general methods for protein purification. Following autocatalytic excision of tagged intein from protein allows to obtain pure protein without formyl-methionin residue on its N-terminus. New method for two step protein purification on the base of protein splicing phenomena has been created. Using modified intein Mxe GyrA extra pure human growth hormone with minimal lost and with native N-terminus was obtained.
Starokadomskiĭ
P L
PL
Okunev
O V
OV
Irodov
D M
DM
Kordium
V A
VA
D001709:Biotechnology / Q000379:methods; D019382:Human Growth Hormone / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D047668:Inteins / Q000235:genetics*; D019154:Protein Splicing; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*
D011993:Recombinant Fusion Proteins; D019382:Human Growth Hormone