Optical diffraction tomography for high resolution live cell imaging.
We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.
Sung
Yongjin
Y
GR Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Choi
Wonshik
W
Fang-Yen
Christopher
C
Badizadegan
Kamran
K
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D000465:Algorithms; D016477:Artifacts; D002477:Cells / Q000166:cytology* / Q000648:ultrastructure; D003952:Diagnostic Imaging / Q000379:methods; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008027:Light; D008029:Lighting; D008857:Microscopy, Interference / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods; D055095:Optics and Photonics; D012031:Refractometry; D012399:Rotation; D012680:Sensitivity and Specificity; D014054:Tomography; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*