Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies.
A system for vascular hollow fiber bio-artificial pancreas development, optimization and in vitro testing was implemented and operated in a simple and fully described manner, allowing other researchers to test a variety of experimental conditions (different biomaterials, biologic tissue, addition of proteins or other adjuvants). In this work, a polysulfone hollow fiber was used as bioprotective material. Two different cell sources were co-immobilized with agarose microspheres in and experimented with the membrane device: rat islets of Langerhans and mouse beta-TC-3 insulinoma cells. The results obtained with islets of Langerhans were used as islet comparable insulin-release data. Beta-TC-3 cells were mainly used in these studies, due to higher control and reproducibility of cell number and behavior: addition of hemoglobin was beneficial for sustained cell viability, especially during cell insertion in the device (viability assessed by beta-TC-3 lactate dehydrogenase activity in the recirculating culture medium); cells did not adhere to the polysulfone membrane (assessed by SEM observation of membrane samples from dynamic cultures). Comparable device functionality and insulin-release results were attained with both cell types: device functionality was maintained for 7-9 days and maximum insulin-release during dynamic glucose challenges were 2.6 x 10(-3)+/-7 x 10(-5)microU/beta-cell x 8 h, with islets, and 9.3 x 10(-4)+/-2 x 10(-5)microU/beta-cell x 2 h, with beta-TC-3 cells.
Silva
A I
AI
Institute of Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Lisboa, Portugal.
Mateus
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D000818:Animals; D021522:Bioartificial Organs; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D018914:Cells, Immobilized; D003470:Culture Media; D004867:Equipment Design; D005947:Glucose / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D008954:Models, Biological; D019397:Pancreas, Artificial; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013450:Sulfones / Q000737:chemistry*
D003470:Culture Media; D006454:Hemoglobins; D007328:Insulin; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D007770:L-Lactate Dehydrogenase; D005947:Glucose