Tailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis.
Tailoring steps are often important for the activity of mature antibiotics. Here, we report that novel decarboxylated FR-008/candicidin derivatives were obtained from the P450 monooxygenase gene fscP mutant of Streptomyces sp. strain FR-008. The toxicity of decarboxylated FR-008/candicidin derivatives has been shown to be greatly reduced compared to that of wild-type FR-008/candicidin.
Chen
Shi
S
Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Mao
Xiangzhao
X
Shen
Yaling
Y
Zhou
Yongjun
Y
Li
Jialiang
J
Wang
Lianrong
L
Tao
Xinyi
X
Yang
Liang
L
Wang
Yuxiao
Y
Zhou
Xiufen
X
Deng
Zixin
Z
Wei
Dongzhi
D
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000633:toxicity; D002174:Candicidin / Q000096:biosynthesis* / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects; D017353:Gene Deletion; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D011817:Rabbits; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002174:Candicidin; D006899:Mixed Function Oxygenases