Effects of air transportation cause physiological and biochemical changes indicative of stress leading to regulation of chaperone expression levels and corticosterone concentration.
Laboratory animals generally experience numerous unfamiliar environmental and psychological influences such as noises, temperatures, handling, shaking, and smells during the process of air transportation. To investigate whether stress induced by air transportation affects stress-related factors in animals, the levels of hormone and chaperone protein were measured in several tissues of F344 rats transported for 13 h and not transported. Herein, we conclude that the levels of corticosterone, HSP70, and GRP78 were significantly increased in the transported group compare to not transported group, but they were rapidly restored to the not transported group level after a recovery period of one week. However, the magnitude of induction and restoration levels of these factors varied depending on the tissue type. Thus, these results suggest that air transportation should be considered for the improvement of laboratory animal health and to reduce the incidence of laboratory animal stress.
Shim
SunBo
S
Laboratory Animal Resources Team, National Institute of Toxicological Research, Korean FDA.
Lee
SeHyun
S
Kim
ChuelKyu
C
Kim
ByoungGuk
B
Jee
SeungWan
S
Lee
SuHae
S
Sin
JiSoon
J
Bae
ChangJoon
C
Woo
Jong-Min
JM
Cho
JungSik
J
Lee
EonPil
E
Choi
HaeWook
H
Kim
HongSung
H
Lee
JaeHo
J
Jung
YoungJin
Y
Cho
ByungWook
B
Chae
KabRyong
K
Hwang
DaeYoun
D
D000337:Aerospace Medicine; D000401:Aircraft; D000818:Animals; D001522:Behavior, Animal; D003345:Corticosterone / Q000097:blood*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood*; D006360:Heat-Shock Proteins / Q000097:blood*; D008297:Male; D018832:Molecular Chaperones / Q000097:blood*; D051381:Rats; D011916:Rats, Inbred F344; D012737:Sex Factors; D013315:Stress, Psychological / Q000097:blood* / Q000209:etiology
D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D003345:Corticosterone