Important roles of glycosylphosphatidylinositol (GPI)-specific phospholipase D and some GPI-anchored proteins in the pathogenesis of hepatocellular carcinoma.
OBJECTIVE
To investigate the roles of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in the pathogenesis of hepatocellular carcinoma (HCC).


METHODS
The expression of the GPI-PLD in HCC was determined. The GPI-PLD gene was stably transfected in HepG2 cells and the proliferation of these cells was detected; CD55, CD59 and apoptotic cells were also analyzed.


RESULTS
The serum GPI-PLD activities, the protein and mRNA levels of GPI-PLD in HCC patients were decreased by 40%, 60% and 56%, respectively. The killing rate of CDC against the positive clone cells was significantly increased, but the proliferative capacity was obviously decreased. The apoptotic rate in positive clones was increased.


CONCLUSIONS
The expression of GPI-PLD decreases in HCC patients. The over-expression of GPI-PLD in HepG2 cells increases their sensitivity to CDC killing, impairs proliferative capacity and promotes apoptosis.
Tang
Jian-Hua
JH
Department of Biochemistry, School of Biological Sciences and Technology, Central South University, 172 Tong Zi Po Road, Changsha, Hunan 410013, PR China. miket561@xysm.net
He
Wang-Jiao
WJ
Huang
He
H
Tan
Chao-Chao
CC
Duan
Qiong
Q
Wang
Kai-Jia
KJ
Yuan
Xian-Yu
XY
Zhu
Xu-Jin
XJ
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis; D018958:CD55 Antigens / Q000378:metabolism; D018957:CD59 Antigens / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D003165:Complement System Proteins / Q000276:immunology; D053938:DNA Fragmentation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000201:enzymology* / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D018958:CD55 Antigens; D018957:CD59 Antigens; D017261:Glycosylphosphatidylinositols; D003165:Complement System Proteins; D000469:Alkaline Phosphatase; D010739:Phospholipase D; C065017:glycoprotein phospholipase D