title: [Experimental studies on anti-mouse hepatocellular carcinoma effects of cisplatin combined with exosomes].
abstract: OBJECTIVE
  To study the anti-tumor effects and mechanisms of cisplatin(DDP) combined with exosomes.
  
  
  METHODS
  The cell-growth inhibition of DDP on H(22); cells was analyzed by MTT assay.The mice were immunized by H(22); cell-derived exosomes. The exosomes elicited CTL-specific cytotoxicity and DDP enhanced cytotoxicity in combination with CTL were detected by (3)H-TdR release assay. The effect of DDP on mRNA and protein levels of Fas in H(22); was analyzed using RT-PCR and Western blot.The expression of FasL on spleen lymphocyte was determined by RT-PCR. BALB/c mice inoculated with hepatoma carcinoma cell line H(22); were used as tumor models.The mice received exosomes solely or in combination with DDP.The survival of the mice was observed.
  
  
  RESULTS
  DDP inhibited H(22); cell in a dose-dependant manner. The exosomes elicited CTL-specific cytotoxicity was enhanced by DDP (P<0.05). RT-PCR and Western blot showed Fas increased gradually after administering DDP on H(22); cells.RT-PCR also indicated the mRNA level of FasL on mice spleen lymphocyte increased after immunized by exosomes. Compared with other groups, the combination group(DDP plus exosomes)could statistically prolong the survival time (P<0.05).
  
  
  CONCLUSIONS
  The therapy of DDP combined with exosomes had significant synergistic effect against tumor. The mechanism of synergistic effect includes enhancement of CTL activity.
authors.affiliation: Department of Pathophsiology, Institute of Stem Cell and Tissue Engineering, Chongqing Medical University, China. wangshaohua20006@163.com
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Zi-Wu
authors.initials: ZW
authors.lastname: Xiang
authors.forename: Wei-Ke
authors.initials: WK
authors.lastname: Fan
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000276:immunology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use*; D004195:Disease Models, Animal; D055354:Exosomes / Q000276:immunology*; D005260:Female; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D019014:fas Receptor; D002945:Cisplatin