title: Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions.
abstract: Conventional NMR approaches to detect weak protein binding and aggregation are hindered by the increased viscosity brought about by crowding. We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Conggang
authors.initials: C
authors.lastname: Li
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Pielak
mesh_terms: D000818:Animals; D002417:Cattle; D003198:Computer Simulation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D014783:Viscosity
chemical_list: D012710:Serum Albumin, Bovine