The effect of aberrant maspin expression on the invasive ability of pancreatic ductal adenocarcinoma cells.
Maspin is expressed aberrantly in pancreatic ductal adenocarcinoma (PDAC), and its function is still unknown. To explore the role of maspin in PDAC, we constructed wild-type maspin-expressing Panc-1 clones (Panc-1-maspin) by transfecting maspin cDNA into Panc-1, a PDAC-derived cell line that lacks maspin expression. As a control, mock-transfected clones (Panc-1-mock) were constructed using the same method. The invasive ability of the stable transfectants was evaluated with an in vitro invasion assay. The ability of Panc-1-maspin cells to migrate through a Matrigel-coated filter was significantly reduced compared to that of Panc-1-mock cells (p=0.012). In addition, we identified the c.1022A>G variant of maspin in human PDAC cells; however, this polymorphism was not involved in the clinical characteristics of PDAC nor did it alter the invasive ability of PDAC cells. The results of the present study indicate that maspin suppresses the invasive ability of PDAC cells.
Hong
Sung Noh
SN
Konkuk University Hospital, Konkuk University School of Medicine, Seoul, Korea.
Lee
Jong Kyun
JK
Choe
Won Hyoek
WH
Ha
Hye Young
HY
Park
Kyoungsook
K
Sung
In Kyung
IK
Lee
Kyu Taek
KT
Kim
Jae J
JJ
Rhee
Jong Chul
JC
D014408:Biomarkers, Tumor / Q000032:analysis; D015152:Blotting, Northern; D015153:Blotting, Western; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D014408:Biomarkers, Tumor; C085344:SERPIN-B5; D015843:Serpins