[Effect of hydrostatic pressure on ultrastructure of synovial fibroblasts from rat temporomandibular joint].
OBJECTIVE
To investigate the role of apoptosis in the remodeling of temporomandibular joint (TMJ) under pressure.


METHODS
Synovial fibroblasts obtained from rat temporomandibular joint were subjected to different hydrostatic pressure for 12 h. Changes of ultrastructure were observed by transmission electron microscope.


RESULTS
At 30 kPa, the ultrastructure of synovial fibroblasts showed no obvious changes. At 60 kPa, the chromatin was condensated, the baryon took on crescent and the mitochondria seemed varicose. At 90 kPa, the apoptosis-like body was wrapped by membrane and embedded in the high density chromatin.


CONCLUSIONS
Apoptosis-like change took place in ultrastructure of synovial fibroblasts under hydrostatic pressure, and the degree of the change was related to the hydrostatic pressure exerted.
Wu
Meng-Jie
MJ
Department of Orthodontics, Hospital of Stomatology, Zhejiang University, Hangzhou, China.
Feng
Jian-Ying
JY
Gu
Zhi-Yuan
ZY
D000818:Animals; D017209:Apoptosis; D005347:Fibroblasts / Q000648:ultrastructure*; D006874:Hydrostatic Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology; D013704:Temporomandibular Joint / Q000166:cytology