title: Mutagenesis of the nucleocapsid protein of Nipah virus involved in capsid assembly.
abstract: The nucleocapsid protein of Nipah virus produced in Escherichia coli assembled into herringbone-like particles. The amino- and carboxy-termini of the N protein were shortened progressively to define the minimum contiguous sequence involved in capsid assembly. The first 29 aa residues of the N protein are dispensable for capsid formation. The 128 carboxy-terminal residues do not play a role in the assembly of the herringbone-like particles. A region with amino acid residues 30-32 plays a crucial role in the formation of the capsid particle. Deletion of any of the four conserved hydrophobic regions in the N protein impaired capsid formation. Replacement of the central conserved regions with the respective sequences from the Newcastle disease virus restored capsid formation.
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Swee Tin
authors.initials: ST
authors.lastname: Ong
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.|Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
authors.affiliation: Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, UTAR Complex, Jalan Genting Kelang, 53300 Setapak, Kuala Lumpur, Malaysia.
authors.forename: Chiew Ling
authors.initials: CL
authors.lastname: Kho
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Janna Ong
authors.initials: JO
authors.lastname: Abdullah
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.|Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Wen Siang
authors.initials: WS
authors.lastname: Tan
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000502:physiology*; D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers; D004926:Escherichia coli / Q000821:virology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D045405:Nipah Virus / Q000235:genetics* / Q000648:ultrastructure; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000648:ultrastructure; D017384:Sequence Deletion
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D019590:Nucleocapsid Proteins