Kinetic complexity of the global response to glucocorticoid receptor action.
We have characterized the kinetic response of gene targets throughout the murine genome to transcriptional modulation by the glucocorticoid receptor (GR). In contrast to a model in which multiple genes are either repressed or activated during the GR response, the vast majority of responsive genes are subject to complex regulation profiles, frequently with alternate activation and repression phases. We also observe that GR binding at response elements does not always correlate with the target gene response profile. Thus, the cellular response to GR stimulation involves a highly orchestrated series of regulatory actions and not simply a binary response to hormone.
John
Sam
S
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-5055, USA.
Johnson
Thomas A
TA
Sung
Myong-Hee
MH
Biddie
Simon C
SC
Trump
Saskia
S
Koch-Paiz
Christine A
CA
Davis
Sean R
SR
Walker
Robert
R
Meltzer
Paul S
PS
Hager
Gordon L
GL
D000818:Animals; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation / Q000235:genetics; D007700:Kinetics; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics* / Q000502:physiology; D020218:Response Elements / Q000235:genetics* / Q000502:physiology; D013997:Time Factors; D015533:Transcriptional Activation / Q000235:genetics / Q000502:physiology
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D012319:RNA Polymerase II