title: The interaction of white spot syndrome virus envelope protein VP28 with shrimp Hsc70 is specific and ATP-dependent.
abstract: White spot syndrome virus (WSSV) is one of the most devastating pathogens of shrimps and other crustaceans. In a previous study, we demonstrated that the major envelope protein VP28 of WSSV was involved in the attachment and penetration into shrimp cells. Here we showed that heat-shock cognate protein 70 (Hsc70) of shrimp was a binding partner of VP28 during virus infection. Hsc70 expression was enhanced by WSSV infection at the early stage and peaked at 12h post-infection and decreased drastically at the late stage. In shrimp haemocytes, both VP28 and Hsc70 proteins localized in the cytoplasm and their association was specific, ATP-dependent and Hsc70 concentration dependent. Moreover, direct VP28-Hsc70 association required both ATPase domain and peptide binding domain of Hsc70. The data obtained will help elucidate the role of VP28 protein in the process of virus infection.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Bayi road, Hubei, Wuhan 430072, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Deng
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zou
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism*; D006434:Hemocytes / Q000378:metabolism; D033561:Penaeidae / Q000821:virology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D050883:HSC70 Heat-Shock Proteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D000255:Adenosine Triphosphate