A mutation in the mouse Chd2 chromatin remodeling enzyme results in a complex renal phenotype.
OBJECTIVE
Glomerular diseases are the third leading cause of kidney failure worldwide, behind only diabetes and hypertension. The molecular mechanisms underlying the cause of glomerular diseases are still largely unknown. The identification and characterization of new molecules associated with glomerular function should provide new insights into understanding the diverse group of glomerular diseases. The Chd2 protein belongs to a family of enzymes involved in ATP-dependent chromatin remodeling, suggesting that it likely functions as an epigenetic regulator of gene expression via the modification of chromatin structure.


METHODS
In this study, we present a detailed histomorphologic characterization of mice containing a mutation in the chromodomain helicase DNA-binding protein 2 (Chd2).


RESULTS
We show that Chd2-mutant mice present with glomerulopathy, proteinuria, and significantly impaired kidney function. Additionally, serum analysis revealed decreased hemoglobin and hematocrit levels in Chd2-mutant mice, suggesting that the glomerulopathy observed in these mice is associated with anemia.


CONCLUSIONS
Collectively, the data suggest a role for the Chd2 protein in the maintenance of kidney function.
Marfella
Concetta G A
CG
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Mass. 01655, USA.
Henninger
Nils
N
LeBlanc
Scott E
SE
Krishnan
Namrata
N
Garlick
David S
DS
Holzman
Lawrence B
LB
Imbalzano
Anthony N
AN
D000818:Animals; D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D044127:Epigenesis, Genetic; D015433:Glomerulonephritis, Membranous; D007668:Kidney / Q000502:physiology / Q000503:physiopathology; D007674:Kidney Diseases / Q000235:genetics*; D051379:Mice; D009154:Mutation; D010641:Phenotype; D011507:Proteinuria
C514205:Chd2 protein, mouse; D004268:DNA-Binding Proteins