title: Free thiols in human spermatozoa: are Na+/K+-ATPase, Ca2+-ATPase activities involved in sperm motility through peroxynitrite formation?
abstract: The aim of the present study was to measure free thiols content, Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities in human spermatozoa of asthenozoospermic patients and normospermic donors, and evaluate any influence on kinetic sperm features, as well as correlation with peroxynitrite. In fact, membrane integrity and its composition are the basic characteristics of the sperm membrane; thus, it is evident that its composition is an important factor for membrane functions that can be modified upon oxidation. A total of 70 infertile patients affected by idiopathic asthenozoospermia and 25 normal fertile donors were enrolled. Control spermatozoa exhibited Na(+)/K(+)-ATPase, and Ca(2+)-ATPase activities, cytoplasmic Ca(2+) concentration and free -SH content significantly higher than those of asthenozoospermic patients (P < 0.0001). Moreover, positive associations were found between Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities and total sperm motility and sperm kinetic features, whereas negative associations were found between peroxynitrite and Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities, and total SH content. Peroxynitrite is able to reduce Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities and intracellular Ca(2+) concentration, through possible depletion of free thiols content. Decrease in motility and loss of sperm function in idiopathic asthenozoospermia can be attributed to these sulphydryl groups, important entities of the sperm membrane.
authors.affiliation: Institute of Biochemistry, School of Medicine, Polytechnic University of Marche, Ancono, Italy. vignini2@libero.it
authors.forename: Arianna
authors.initials: A
authors.lastname: Vignini
authors.forename: Eddi
authors.initials: E
authors.lastname: Buldreghini
authors.forename: Laura
authors.initials: L
authors.lastname: Nanetti
authors.forename: Salvatore
authors.initials: S
authors.lastname: Amoroso
authors.forename: Marco
authors.initials: M
authors.lastname: Boscaro
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ricciardo-Lamonica
authors.forename: Laura
authors.initials: L
authors.lastname: Mazzanti
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Balercia
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000378:metabolism / Q000473:pathology; D000252:Calcium-Transporting ATPases / Q000378:metabolism / Q000502:physiology*; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D030421:Peroxynitrous Acid / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D055101:Semen Analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D013438:Sulfhydryl Compounds; D030421:Peroxynitrous Acid; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase