Molecular characterization of hepatitis A virus isolates from Goiânia, Gioás, Brazil.
Hepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here, the HAV genotypes were analyzed of 18 isolates circulating between 1996-2001 in Goiânia, state of Goiás, Brazil. Viral RNA was extracted from 18 serum samples and amplified (RT-PCR/nested-PCR), followed by the genomic sequencing of the VP1/2A junction region of the HAV genome. Sequences of 168 nucleotides were compared and analyzed using the BLAST N, Clustal X and PAUP v. 4.10b programs. All samples were classified as genotype I, with 10 belonging to subgenotype IA and eight to subgenotype IB. The subgenotype IA isolates showed greater diversity than the subgenotype IB isolates at the nucleotide level. Elevated identity values were found between isolates obtained in this study and those from other regions of the world, including Brazil, highlighting the high conservation among different isolates of this virus. However, changes in the HAV subgenotype circulation could also be observed during the evaluated period.
Fiaccadori
Fabíola Souza
FS
Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil. fabiola@iptsp.ufg.br
Pereira
Maristela
M
Coelho
Alexandre Siqueira Guedes
AS
Borges
Ana Maria Tavares
AM
Parente
Juliana Alves
JA
Soares
Célia Maria de Almeida
CM
Cardoso
Divina das Dôres de Paula
Dd
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D005260:Female; D006506:Hepatitis A / Q000821:virology*; D030041:Hepatitis A virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D012367:RNA, Viral