title: Potential-dependent permeabilization of plasma membrane by the peptide BTM-P1 derived from the Cry11Bb1 protoxin.
abstract: The peptide BTM-P1, which is derived from the amino acid sequence of the Cry11Bb1 protoxin, is able to permeabilize mitochondrial membranes and reveals antimicrobial activity. In this work we demonstrated that the permeabilizing activity of BTM-P1 for the plasma membrane of rat red blood cells increased in a dose-dependent manner for the concentration range of 1-4 microg/ml. Using osmotic protectants, the radius of pores formed at 4 microg/ml BTM-P1 was determined as 0.8 nm for 5 min hemolysis data, 0.7 nm for 5 min decrease in light dispersion of the cell suspension and 0.5 nm for the light dispersion slope measurements. The permeabilizing activity of 1 microg/ml peptide was increased by valinomycin-induced plasma membrane potential, especially under moderately hypotonic conditions. These results might explain the antimicrobial activity of BTM-P1 and support the hypothesis of potential-dependent and pro-apoptotic character of toxicity of naturally proteolysed Cry11Bb1 protoxin for epithelial cells of mosquito larvae midgut.
authors.affiliation: Escuela de Física, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Medellín, Calle 59A, No 63-20, Medellín, Colombia.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Arias
authors.forename: Sergio
authors.initials: S
authors.lastname: Orduz
authors.forename: Victor V
authors.initials: VV
authors.lastname: Lemeshko
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001427:Bacterial Toxins / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D006461:Hemolysis; D008564:Membrane Potentials; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D023181:Antimicrobial Cationic Peptides; C501422:BTM-P1 peptide; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C115468:CRY11BB protein, Bacillus thuringiensis