Oxidative stress involvement in Physalis angulata-induced apoptosis in human oral cancer cells.
In this report, we investigated the role of oxidative stress in Physalis angulata-induced apoptosis of human oral cancer cells. P. angulata-induced apoptosis was characterized by nuclear morphological changes, membrane blebbing and activation of caspase-9. Exposure of HSC-3 cells to P. angulata caused production of reactive oxygen species and up-regulation of oxidative stress markers heme oxygenase-1 (HO-1), superoxide dismutase (SOD), heat shock protein 70 (HSP70) and caspase-4. Down-regulation of HO-1, SOD and HSP70 proteins expression by attenuation of oxidative stress, pretreatment with glutathione or N-acetylcysteine, significantly decreased P. angulata-triggered cell death. The present study also demonstrated that the mitochondria and the endoplasmic reticulum are the targets of P. angulata in HSC-3 cells. Our results revealed that: (1) reactive oxygen species may play a dominant role in this process, (2) P. angulata induces oxidative stress in HSC-3 cells, (3) P. angulata-initiated apoptosis is caused through oxidative stress-dependent induction of heme oxygenase-1, Cu/Zn SOD and HSP70 proteins expression and (4) antioxidants inhibited P. angulata-induced cell death through inhibition of the proteins expression of HO-1, Cu/Zn SOD and HSP70.
Lee
H-Z
HZ
School of Pharmacy, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
Liu
W-Z
WZ
Hsieh
W-T
WT
Tang
F-Y
FY
Chung
J-G
JG
Leung
Henry W-C
HW
D002455:Cell Division; D045744:Cell Line, Tumor; D016195:G2 Phase; D006801:Humans; D008856:Microscopy, Fluorescence; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D032321:Physalis / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D010936:Plant Extracts; D017382:Reactive Oxygen Species