title: In vivo and in vitro antiviral activities of calycosin-7-O-beta-D-glucopyranoside against coxsackie virus B3.
abstract: Calycosin-7-O-beta-D-glucopyranoside (CCGR) is the main isoflavonoid compound isolated from Astragalus membranaceus var. Mongholicu (BGE.) HSIAO, a Chinese herb medicine traditionally used to treat viral myocarditis. In this study, its antiviral activities against coxsackie virus B3 (CVB3) causing myocarditis were investigated. In vitro assay showed that CCGR displayed a low cytotoxicity and effectively inhibited CVB3-mediated cytopathic effects on Vero cells with an IC(50) value of 25 microg/ml. In an acute myocarditis murine model, treatment with 24 mg/kg CCGR for 14 d significantly improved the survival rate of mice infected with CVB3, alleviated pathological damages of cardiac muscles in the myocarditis mice, reduced the virus titers in the heart, decreased heart indexes and improved left ventricular function. These results showed that CCGR exerted significant antiviral activities against CVB3 both in vitro and in vivo, and identified CCGR as one of active ingredients in Astragalus membranaceus for the treatment of viral myocarditis.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guan
authors.initials: G
authors.lastname: Ye
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000627:therapeutic use*; D002522:Chlorocebus aethiops; D003384:Coxsackievirus Infections / Q000188:drug therapy*; D004452:Echocardiography / Q000379:methods; D029822:Enterovirus B, Human; D005960:Glucosides / Q000737:chemistry / Q000627:therapeutic use*; D007529:Isoflavones / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000166:cytology; D013997:Time Factors; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D005960:Glucosides; D007529:Isoflavones; C488311:calycosin-7-O-glucopyranoside