Identification of a 27 kDa protein in patients with anaphylactic reactions to mango.
Mango fruit has become increasingly popular in recent years. We report on 2 patients who developed anaphylactic reactions after the ingestion of fresh mango. Allergy to mango was confirmed by a positive skin prick test result and positive cellular allergen stimulation test results. Neither of the patients had detectable mango-specific immunoglobulin (Ig) E levels. Results were validated by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblotting and analyzed using Quantiscan.We identified 2 major allergens with a molecular weight of 27 kDa in both patients, in addition to a 15 kDa allergen in 1 patient and a 32 kDa allergen in the other. Currently available IgE systems seem to be lacking these mango allergens and as such are probably unsuitable for diagnosing type 1 sensitization to mango. Skin prick testing with fresh mango fruit therefore seems to be a much more reliable test method for clinical practice.
Renner
R
R
Clinic for Dermatology, Venereology and Allergology, University Hospital of Leipzig, Leipzig, Germany. regina.renner@medizin.uni-leipzig.de
Hipler
C
C
Treudler
R
R
Harth
W
W
Süss
A
A
Simon
J C
JC
D000707:Anaphylaxis / Q000175:diagnosis* / Q000209:etiology; D052179:Antigens, Plant / Q000032:analysis*; D005260:Female; D005512:Food Hypersensitivity / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D031022:Mangifera / Q000276:immunology*; D008875:Middle Aged; D012882:Skin Tests; D055815:Young Adult
D052179:Antigens, Plant; D007073:Immunoglobulin E