Spectrophotometric determination of quinolone antibiotics by an association complex formation with aluminum(III) and erythrosin.
A simple and sensitive spectrophotometric method for the determination of quinolone antibiotics was established based on an association complex formation with aluminum(III) and erythrosin. In the determination of ofloxacin as a quinolone antibiotic, Beer's law is obeyed in the range of 0.1 - 3.2 microg ml(-1), with an effective molar absorptivity at 555 nm and the relative standard deviation being 1.2 x 10(5) L mol(-1) cm(-1) and 0.9% (n = 6). This method was successfully applied to the assay of quinolone antibiotics in pharmaceutical preparations.
Yamaguchi
Takako
T
Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1094, Japan.
Nakao
Masahiro
M
Nakahara
Ryosuke
R
Nishioka
Yuka
Y
Ikeda
Chiho
C
Fujita
Yoshikazu
Y
D000535:Aluminum / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004923:Erythrosine / Q000737:chemistry; D015242:Ofloxacin / Q000032:analysis; D011804:Quinolines / Q000032:analysis*; D013057:Spectrum Analysis
D000900:Anti-Bacterial Agents; D011804:Quinolines; D015242:Ofloxacin; D000535:Aluminum; D004923:Erythrosine