Selective fluorescence labeling of lipids in living cells.
Click chemistry in vivo: Three phosphatidic acid derivatives with alkyne groups in their fatty acid chains were synthesized and incorporated into mammalian cell membranes. Copper(I)-catalyzed and strain-promoted azide-alkyne cycloaddition reactions were used for their visualization (see schematic representation and fluorescence microscopic image).
Neef
Anne B
AB
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Schultz
Carsten
C
D000480:Alkynes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D002478:Cells, Cultured; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence
D000480:Alkynes; D001386:Azides; D005456:Fluorescent Dyes; D008055:Lipids; D003300:Copper