[Genetic diversity of ancient tea gardens and tableland tea gardens from Yunnan Province as revealed by AFLP marker].
This study was conducted to evaluate the genetic diversity within and among the plants of four ancient tea gardens and two tableland tea gardens form Yunnan Province, China by AFLP technique. The percentage of polymorphic loci (P) of the plants from six tea gardens was 92.31%. The genetic diversity within the six gardens demonstrated by Nei cents genetic diversity (He) was estimated to be 0.1366, while Shannon indices (Ho) were 0.2323. The percentage of polymorphic loci of the four ancient tea populations was 45.55% on average, with a range of 36.44% (Mengsong) to 59.11% (Mengla). But the percentages of polymorphic loci of the plants from two tableland gardens were 13.77% (Yunkang 10) and 24.2% (Menghai Daye), respectively. There was a great genetic difference between ancient tea gardens and tableland tea gardens. The genetic diversity among the plants of the ancient tea garden was higher than those of the sexual tableland tea garden and the clone tableland tea garden based on P valve. The four ancient tea gardens and two tableland gardens could be differentiated with AFLP markers. The results show that AFLP marker is an effective tool in the discrimination of tea germplasm, as well as sundried green tea.
Ji
Peng-Zhang
PZ
College of Life Sciences, Yunnan University, Kunming 650091, China. pengzhji@126.com
Jiang
Hui-Bing
HB
Huang
Xing-Qi
XQ
Zhang
Jun
J
Liang
Min-Zhi
MZ
Wang
Ping-Sheng
PS
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002681:China; D014644:Genetic Variation / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013662:Tea / Q000145:classification / Q000235:genetics*
D013662:Tea