title: The genomic architecture of segmental duplications and associated copy number variants in dogs.
abstract: Structural variation is an important and abundant source of genetic and phenotypic variation. Here we describe the first systematic and genome-wide analysis of segmental duplications and associated copy number variants (CNVs) in the modern domesticated dog, Canis familiaris, which exhibits considerable morphological, physiological, and behavioral variation. Through computational analyses of the publicly available canine reference sequence, we estimate that segmental duplications comprise approximately 4.21% of the canine genome. Segmental duplications overlap 841 genes and are significantly enriched for specific biological functions such as immunity and defense and KRAB box transcription factors. We designed high-density tiling arrays spanning all predicted segmental duplications and performed aCGH in a panel of 17 breeds and a gray wolf. In total, we identified 3583 CNVs, approximately 68% of which were found in two or more samples that map to 678 unique regions. CNVs span 429 genes that are involved in a wide variety of biological processes such as olfaction, immunity, and gene regulation. Our results provide insight into mechanisms of canine genome evolution and generate a valuable resource for future evolutionary and phenotypic studies.
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Nicholas
authors.forename: Ze
authors.initials: Z
authors.lastname: Cheng
authors.forename: Mario
authors.initials: M
authors.lastname: Ventura
authors.forename: Katrina
authors.initials: K
authors.lastname: Mealey
authors.forename: Evan E
authors.initials: EE
authors.lastname: Eichler
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Akey
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D055028:Comparative Genomic Hybridization; D004285:Dogs / Q000235:genetics*; D018628:Gene Dosage; D020440:Gene Duplication; D014644:Genetic Variation / Q000502:physiology*; D016678:Genome / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D013045:Species Specificity