Separation of complex mixtures of fluorobenzoic acids by capillary electrophoresis.
Fluorobenzoic acids are important intermediates in the synthesis of antibacterial drugs. Conditions for the separation of mixtures of twenty-five acids by CE have been optimized. A set of conditions with phosphate buffer (pH 3.1), successive multiple ionic-polymer layer (SMIL) coating capillary, and negative separation voltage provided a short time separation based on the difference in the acidic dissociation constant (pK(a)) of the sample acids. Addition of ACN to the separation solution improved the selectivity resulting in a broader distribution of migration times of the samples. The addition of tetradecyltrimethylammonium chloride below the CMC was also effective in changing the migration pattern of fluorobenzoic acids. Furthermore, practical utility was demonstrated through quantitative studies on the optimized condition including the durability of the SMIL coating.
Isemura
Tsuguhide
T
Analytical Science & TechnologyFunction, Asahi Glass Co., Ltd.Research Center, Yokohama,Kanagawa, Japan. tsuguhide-isemura@agc.co.jp
Kitagawa
Fumihiko
F
Otsuka
Koji
K
D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D017611:Fluorine Compounds / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012822:Silicon Dioxide / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
D001565:Benzoates; D003505:Cyclodextrins; D017611:Fluorine Compounds; D013501:Surface-Active Agents; D012822:Silicon Dioxide