title: Hepatitis B virus genotype in Iranian patients with hepatocellular carcinoma.
abstract: OBJECTIVE
  Chronic hepatitis B virus (HBV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC). HBV appears to be the most common cause of HCC in Iran. To date, no study has been carried out on the HBV genotype in Iranian HCC patients. This study was undertaken to determine the HBV genotype in Iranian patients with HCC.
  
  
  METHODS
  Paraffin-embedded tissue samples from 40 patients (31 males and nine females) with HBV-associated HCC were collected from different pathology centers during 2000-2007. Genotyping of HBV was performed by nested PCR-mediated amplification of the target sequence. PCR products were sequenced, and the genotype of each HBV sequence was determined by comparison with sequences of known genotypes in the GenBank. A phylogenetic tree was constructed.
  
  
  RESULTS
  Phylogenetic analysis revealed that all of the HBV isolates were clustered in genotype D.
  
  
  CONCLUSIONS
  Our results concur with other reports from Iran, all showing that genotype D is the only detectable genotype in the different clinical forms of HBV infection in this country.
authors.affiliation: Clinical Research Department, Pasteur Institute of Iran, No. 69, Pasteur Ave., Tehran 13164, Iran. aaghakhani@pasteur.ac.ir
authors.forename: Arezoo
authors.initials: A
authors.lastname: Aghakhani
authors.forename: Rasool
authors.initials: R
authors.lastname: Hamkar
authors.forename: Nastaran
authors.initials: N
authors.lastname: Zamani
authors.forename: Ali
authors.initials: A
authors.lastname: Eslamifar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Banifazl
authors.forename: Alireza
authors.initials: A
authors.lastname: Saadat
authors.forename: Masoomeh
authors.initials: M
authors.lastname: Sofian
authors.forename: Ladan
authors.initials: L
authors.lastname: Adibi
authors.forename: Nemat
authors.initials: N
authors.lastname: Irani
authors.forename: Matin
authors.initials: M
authors.lastname: Mehryar
authors.forename: Hassan Mohammad Hosseini
authors.initials: HM
authors.lastname: Akbari
authors.forename: Seyed Mohammad Javad
authors.initials: SM
authors.lastname: Purkhayat
authors.forename: Amitis
authors.initials: A
authors.lastname: Ramezani
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004279:DNA, Viral