Determining important regulatory relations of amino acids from dynamic network analysis of plasma amino acids.
The changes in the concentrations of plasma amino acids do not always follow the flow-based metabolic pathway network. We have previously shown that there is a control-based network structure among plasma amino acids besides the metabolic pathway map. Based on this network structure, in this study, we performed dynamic analysis using time-course data of the plasma samples of rats fed single essential amino acid deficient diet. Using S-system model (conceptual mathematical model represented by power-law formalism), we inferred the dynamic network structure which reproduces the actual time-courses within the error allowance of 13.17%. By performing sensitivity analysis, three of the most dominant relations in this network were selected; the control paths from leucine to valine, from methionine to threonine, and from leucine to isoleucine. This result is in good agreement with the biological knowledge regarding branched-chain amino acids, and suggests the biological importance of the effect from methionine to threonine.
Shikata
Nahoko
N
Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Maki
Yukihiro
Y
Nakatsui
Masahiko
M
Mori
Masato
M
Noguchi
Yasushi
Y
Yoshida
Shintaro
S
Takahashi
Michio
M
Kondo
Nobuo
N
Okamoto
Masahiro
M
D000596:Amino Acids / Q000097:blood* / Q000378:metabolism*; D000818:Animals; D008297:Male; D015233:Models, Statistical; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids