title: The bacteriorhodopsin carboxyl-terminus contributes to proton recruitment and protein stability.
abstract: We examined functional and structural roles for the bacteriorhodopsin (bR) carboxyl-terminus. The extramembranous and intracellular carboxyl-terminus was deleted by insertion of premature translation stop codons. Deletion of the carboxyl-terminus had no effect on purple membrane (PM) lattice dimensions, sheet size, or the electrogenic environment of the ground-state chromophore. Removal of the distal half of the carboxyl-terminus had no effect on light-activated proton pumping, however, truncation of the entire carboxyl-terminus accelerated the rates of M-state decay and proton uptake approximately 3.7-fold and severely distorted the kinetics of proton uptake. Differential scanning calorimetry (DSC) and SDS denaturation demonstrated that removal of the carboxyl-terminus decreased protein stability. The DSC melting temperature was lowered by 6 degrees C and the calorimetric enthalpy reduced by 50% following removal of the carboxyl-terminus. Over the time range of milliseconds to hours at least 3 phases were required to describe the SDS denaturation kinetics for each bR construction. The fastest phases were indistinguishable for all bR's, and reflected PM solubilization. At pH 7.4, 20 degrees C, and in 0.3% SDS (w/v) the half-times of bR denaturation were 19.2 min for the wild-type, 12.0 min for the half-truncation and 3.6 min for the full-truncation. Taken together the results of this study suggest that the bR ground state exhibits two "domains" of stability: (1) a core chromophore binding pocket domain that is insensitive to carboxyl-terminal interactions and (2) the surrounding helical bundle whose contributions to protein stability and proton pumping are influenced by long-range interactions with the extramembranous carboxyl-terminus.
authors.affiliation: Department of Chemistry and Biochemistry, Seton Hall University, South Orange, New Jersey 07079, USA. gturner@mac.edu
authors.forename: George J
authors.initials: GJ
authors.lastname: Turner
authors.forename: Shirisha
authors.initials: S
authors.lastname: Chittiboyina
authors.forename: Lauren
authors.initials: L
authors.lastname: Pohren
authors.forename: Kirk G
authors.initials: KG
authors.lastname: Hines
authors.forename: John J
authors.initials: JJ
authors.lastname: Correia
authors.forename: Drake C
authors.initials: DC
authors.lastname: Mitchell
mesh_terms: D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000502:physiology*; D001483:Base Sequence; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry; D019617:Halobacterium salinarum / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000502:physiology*; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011522:Protons; D055672:Static Electricity
chemical_list: D008055:Lipids; D010446:Peptide Fragments; D011522:Protons; D001436:Bacteriorhodopsins