[Conversion of 17 alpha-methyltestosterone to methandrostenolone by the bacterium Pimelobacter simplex VKPM Ac-1632 with the presence of cyclodextrins].
Conditions of conversion of 17 alpha-methyltestosterone to methandrostenolone with the presence of modified beta-cyclodextrins (methylcyclodextrin, hydroxypropylcyclodextrin, and hydroxyethylcyclodextrin) in the steroid:cyclodextrin ratio 1:1 were studied. The experimental solutions of modified beta-cyclodextrins were prepared in deionized water with 5-7% methanol. Under the conditions found to be optimal, 1,2-dehydrogenation of 17 alpha-methyltestosterone was carried out with 2-4 g/l Pimelobacter simplex VKPM Ac-1632 biomass. At the substrate concentration 5-20 g/l, the reaction occurred for 1-15 h without any by-products. The maximum rate of methandrostenolone accumulation was observed with hydroxypropylcyclodextrin. The methylcyclodextrin solution can be reused for complete 17 alpha-methyltestosterone conversion at the concentration 5 g/l.
Druzhinina
A V
AV
Andriushina
V A
VA
Stytsenko
T S
TS
Voĭshvillo
N E
NE
D045930:Anabolic Agents / Q000378:metabolism / Q000494:pharmacology; D001711:Biotransformation / Q000502:physiology; D003505:Cyclodextrins / Q000494:pharmacology*; D008696:Methandrostenolone / Q000378:metabolism* / Q000494:pharmacology; D008777:Methyltestosterone / Q000378:metabolism* / Q000494:pharmacology; D011423:Propionibacteriaceae / Q000254:growth & development*
D045930:Anabolic Agents; D003505:Cyclodextrins; D008696:Methandrostenolone; D008777:Methyltestosterone