Mechanistic insight into the role of transition-state stabilization in cyclophilin A.
Peptidyl prolyl cis-trans isomerases (PPIases) are ubiquitous enzymes in biology that catalyze the cis-trans isomerization of the proline imide peptide bond in many cell signaling pathways. The local change of the isomeric state of the prolyl peptide bond acts as a switching mechanism in altering the conformation of proteins. A complete understanding of the mechanism of PPIases is still lacking, and current experimental techniques have not been able to provide a detailed atomistic picture. Here we have carried out several accelerated molecular dynamics simulations with explicit solvent, and we have provided a detailed description of cis-trans isomerization of the free and cyclophilin A-catalyzed process. We show that the catalytic mechanism of cyclophilin is due mainly to the stabilization and preferential binding of the transition state that is achieved by a favorable hydrogen bond interaction with a backbone NH group. We also show that the substrate in the transition state interacts more favorably with the enzyme than the cis isomer, which in turn interacts more favorably than the trans isomer. The stability of the enzyme-substrate complex is directly correlated with the interaction the substrate makes with a highly conserved arginine residue. Finally, we show that catalysis is achieved through the rotation of the carbonyl oxygen on the N-terminal of the prolyl peptide bond in a predominately unidirectional fashion.
Hamelberg
Donald
D
Department of Chemistry, Georgia State University, Atlanta, Georgia 30302-4098, USA.
McCammon
J Andrew
JA
D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002384:Catalysis; D021984:Cyclophilin A / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D007536:Isomerism; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D009842:Oligopeptides; D001120:Arginine; D021984:Cyclophilin A; D019696:Peptidylprolyl Isomerase