Differential protein expression in heart in UT-B null mice with cardiac conduction defects.
Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at different ages. By two-dimensional electrophoresis-based comparative analysis, we found that more than dozen proteins were modulated (>two-fold) in the myocardium of UT-B null mice. Out of these modulated proteins, troponin T (TNNT2) presented significant changes in UT-B null mice at early stage prior to the development of P-R interval elongation, while the change of atrial natriuretic peptide (ANP) occurred only at late stage in UT-B null mice that had the AV block. These data indicate that UT-B deletion caused the dynamic expression regulation of TNNT2 and ANP, and these proteins may provide new clues to investigate the molecular events involved in cardiac conduction.
Yu
Hao
H
Department of Pathophysiology, Research Center of Prostate Diseases, School of Basic Medicine, Jilin University, Changchun, P. R. China.
Meng
Yan
Y
Wang
Li-Shun
LS
Jin
Xian
X
Gao
Li-Fang
LF
Zhou
Lei
L
Ji
Kun
K
Li
Yang
Y
Zhao
Li-Juan
LJ
Chen
Guo-Qiang
GQ
Zhao
Xue-Jian
XJ
Yang
Baoxue
B
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006329:Heart Conduction System / Q000378:metabolism* / Q000473:pathology*; D066298:In Vitro Techniques; D026901:Membrane Transport Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020107:Troponin T / Q000378:metabolism; D000096982:Urea Transporters
D009320:Atrial Natriuretic Factor; D026901:Membrane Transport Proteins; D020107:Troponin T; D000096982:Urea Transporters