title: [Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata].
abstract: OBJECTIVE
  To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.
  
  
  METHODS
  The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.
  
  
  CONCLUSIONS
  The results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).
authors.affiliation: College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China. xuejp2000@yahoo.com.cn
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Shi
mesh_terms: D029044:Achyranthes / Q000166:cytology* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*
chemical_list: D011134:Polysaccharides