title: Nano-desorption electrospray and kinetic method in chiral analysis of drugs in whole human blood samples.
abstract: A home-made nano-desorption electrospray ionization (nano-DESI) device and the kinetic method were tested in chiral analysis of model clinical samples containing enantiomers of one of three pharmaceutically important compounds: dihydroxyphenylalanine (DOPA), ephedrine and ibuprofen. The initial evaluation of chiral systems was carried out by direct infusion of solution mixtures (analyte/central metal/chiral reference ligand) to a standard electrospray ionization (ESI) source. Cu(II) was used as a central metal for all analytes, L-phenylalanine was applied as a chiral reference ligand for DOPA, whereas L-tryptophan was used for the other two analytes. Then, the ESI source was substituted by a nano-DESI source and dried spots of 1 microL samples of whole human blood spiked with individual drugs were successfully analyzed without any pre-treatment. Irrespective of a laborious initial nano-DESI set-up, the combination of the kinetic method with nano-desorption electrospray has, for the first time, been demonstrated as a promising tool for chiral analysis of drugs in blood samples.
authors.affiliation: Department of Analytical Chemistry, Palacky University, Svobody 8, 77146, Olomouc, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Ranc
authors.forename: Vladimír
authors.initials: V
authors.lastname: Havlícek
authors.forename: Petr
authors.initials: P
authors.lastname: Bednar
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
mesh_terms: D004295:Dihydroxyphenylalanine / Q000097:blood; D004809:Ephedrine / Q000097:blood; D006801:Humans; D007052:Ibuprofen / Q000097:blood; D007700:Kinetics; D036103:Nanotechnology / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D004364:Pharmaceutical Preparations; D004295:Dihydroxyphenylalanine; D004809:Ephedrine; D007052:Ibuprofen