title: Involvement of phospholipase D in the low temperature acclimation-induced thermotolerance in grape berry.
abstract: Both phospholipase D (PLD, EC 3.1.4.4) and salicylic acid (SA) play important roles in response to external stimulation and activating defense system in plants. However, roles of the two signals in plants during the development of thermotolerance induced by low temperature acclimation remain unclear. In the experiment presented in the paper, grape berries (Vitis vinifera L. cv. Chardonnay) were pretreated at 8 degrees C for 3h and then transferred to 45 degrees C for heat stress. Compared with the control without low temperature pretreatment, membrane permeability and malondialdehyde (MDA) contents were reduced and the expression of HSP73 increased in the low temperature-pretreated berries under heat stress. During low temperature acclimation, PLD, SA and HSP73 could be activated. Meanwhile, the expression of HSP73 and the accumulation of free SA induced by low temperature can be inhibited by PLD activity inhibitor. All these results suggest that the activation of PLD is an early response to low temperature, and it is involved in the accumulation of free SA and the development of thermotolerance induced by low temperature acclimation.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
authors.forename: Si-Bao
authors.initials: SB
authors.lastname: Wan
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Tian
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Pan
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Zhan
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Wen
authors.forename: Jian-Ye
authors.initials: JY
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Huang
mesh_terms: D020001:1-Butanol / Q000378:metabolism; D000064:Acclimatization; D002462:Cell Membrane / Q000502:physiology; D003080:Cold Temperature; D005638:Fruit / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D008315:Malondialdehyde / Q000378:metabolism; D010539:Permeability; D010739:Phospholipase D / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*; D027843:Vitis / Q000201:enzymology* / Q000502:physiology
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D008315:Malondialdehyde; D020001:1-Butanol; D010739:Phospholipase D; D020156:Salicylic Acid