title: Real-time PCR and subsequent pyrosequencing for screening of penA mosaic alleles and prediction of reduced susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae.
authors.forename: Magnus
authors.initials: M
authors.lastname: Unemo
authors.forename: Per
authors.initials: P
authors.lastname: Olcén
authors.forename: Hans
authors.initials: H
authors.lastname: Fredlund
authors.forename: Sara
authors.initials: S
authors.lastname: Thulin
mesh_terms: D000483:Alleles; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D002511:Cephalosporins / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D046915:Penicillin-Binding Proteins