title: Regulator trafficking on bacterial transcription units in vivo.
abstract: The trafficking patterns of the bacterial regulators of transcript elongation sigma(70), rho, NusA, and NusG on genes in vivo and the explanation for promoter-proximal peaks of RNA polymerase (RNAP) are unknown. Genome-wide, E. coli ChIP-chip revealed distinct association patterns of regulators as RNAP transcribes away from promoters (rho first, then NusA, then NusG). However, the interactions of elongating complexes with these regulators did not differ significantly among most transcription units. A modest variation of NusG signal among genes reflected increased NusG interaction as transcription progresses, rather than functional specialization of elongating complexes. Promoter-proximal RNAP peaks were offset from sigma(70) peaks in the direction of transcription and co-occurred with NusA and rho peaks, suggesting that the RNAP peaks reflected elongating, rather than initiating, complexes. However, inhibition of rho did not increase RNAP levels within genes downstream from the RNAP peaks, suggesting the peaks are caused by a mechanism other than rho-dependent attenuation.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Mooney
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Davis
authors.forename: Jason M
authors.initials: JM
authors.lastname: Peters
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Rowland
authors.forename: Aseem Z
authors.initials: AZ
authors.lastname: Ansari
authors.forename: Robert
authors.initials: R
authors.lastname: Landick
mesh_terms: D047369:Chromatin Immunoprecipitation; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005798:Genes, Bacterial; D008957:Models, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D014158:Transcription, Genetic
chemical_list: D029968:Escherichia coli Proteins; D012321:DNA-Directed RNA Polymerases