A chromatic explosion: the development and future of multiparameter flow cytometry.
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity.
Chattopadhyay
Pratip K
PK
ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3015, USA. pchattop@mail.nih.gov
Hogerkorp
Carl-Magnus
CM
Roederer
Mario
M
D000818:Animals; D004396:Coloring Agents; D004867:Equipment Design; D005434:Flow Cytometry / Q000379:methods* / Q000639:trends; D005544:Forecasting; D006801:Humans; D007154:Immune System Diseases / Q000175:diagnosis; D013223:Statistics as Topic
D004396:Coloring Agents