title: An liquid chromatography-tandem mass spectrometry assay for determination of trace amount of new antifungal drug iodiconazole in human plasma.
abstract: A simple, rapid and sensitive LC-MS/MS method for determination of trace amount of new antifungal drug iodiconazole in human plasma was developed (1-(1H-1,2,4-triazole)-2-(2,4-diflurophenyl)-3-[N-methyl-N-(3-chlor-benzyl)amino]-2-propanol), was used as internal standard (IS). The analytes were extracted by liquid-liquid extraction with n-hexane after internal standard spiked. The separation was performed by a ZORBAX SB-C(18) column (3.5 microm, 2.1 mm x 100 mm) with an isocratic mobile phase consisting of methanol-water-formic acid (50:50:0.05, v/v/v) at a flow rate of 0.3 mL/min. The lower limit of quantification (LLOQ) was 0.10 ng/mL. This method was successfully used to determine the concentration of iodiconazole in human plasma following dermal administration.
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, No. 415 Fengyang Road, Shanghai 200003, PR China.
authors.forename: Shouhong
authors.initials: S
authors.lastname: Gao
authors.forename: Xia
authors.initials: X
authors.lastname: Tao
authors.forename: Lianna
authors.initials: L
authors.lastname: Sun
authors.forename: Chunquan
authors.initials: C
authors.lastname: Sheng
authors.forename: Wannian
authors.initials: W
authors.lastname: Zhang
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Yun
authors.forename: Jingxian
authors.initials: J
authors.lastname: Li
authors.forename: Haijun
authors.initials: H
authors.lastname: Miao
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000935:Antifungal Agents / Q000097:blood*; D001596:Benzylamines / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014230:Triazoles / Q000097:blood*
chemical_list: C539632:1-(1H-1,2,4-triazole)-2-(2,4-difluorophenyl)-3-(N-methyl-N-(4-iodobenzyl)amino)-2-propanol; D000935:Antifungal Agents; D001596:Benzylamines; D014230:Triazoles