title: Molecular characterization of two types of rDNA units in a single strain of Candida albicans.
abstract: Strains of the opportunistic fungal pathogen Candida albicans vary in the presence or absence of a self-splicing group I intron ribozyme (Ca.LSU) in the 25S rRNA gene on chromosome R. Strains of C. albicans typically either lack or contain this ribozyme. However, some strains have both intron-containing and intronless rRNA genes (rDNA). Pulsed-field gel electrophoresis analysis of undigested and restricted DNA showed at least six different karyotypes among eight independent colonies of such a heteroallelic strain. In each case, the variation was in chromosome R, and was due to changes in the number of rDNA units. In strains with only one type of rDNA, chromosome R also varied considerably. Polymerase chain reaction amplification spanning the rDNA unit demonstrated that intron-containing rDNA units are tandemly arrayed, and are immediately adjacent to intronless units in the same cluster. Both types of units were present in the rDNA clusters of both R chromosomes. Possible explanations of these results are loss of Ca.LSU group I intron through purifying selection and/or a relaxation of the commonly accepted concerted evolution of the rDNA units.
authors.affiliation: Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635, USA.
authors.forename: Karl E
authors.initials: KE
authors.lastname: Miletti-González
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Leibowitz
mesh_terms: D002176:Candida albicans / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D007438:Introns; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S