Edible oil degradation by using yeast coculture of Rhodotorula pacifica ST3411 and Cryptococcus laurentii ST3412.
To develop a microbial treatment of edible oil-contaminated wastewater, microorganisms capable of rapidly degrading edible oil were screened. The screening study yielded a yeast coculture comprising Rhodotorula pacifica strain ST3411 and Cryptococcus laurentii strain ST3412. The coculture was able to degrade efficiently even at low contents of nitrogen ([NH(4)-N]=240 mg/L) and phosphorus sources ([PO(4)-P]=90 mg/L). The 24-h degradation rate of 3,000 ppm mixed oils (salad oil/lard/beef tallow, 1:1 w/w) at 20 degrees C was 39.8%+/-9.9% (means+/-standard deviations of eight replicates). The highest degradation rate was observed at 20 degrees C and pH 8. In a scaled-up experiment, the salad oil was rapidly degraded by the coculture from 671+/-52.0 to 143+/-96.7 ppm in 24 h, and the degradation rate was 79.4%+/-13.8% (means+/-standard deviations of three replicates). In addition, a repetitive degradation was observed with the cell growth by only pH adjustment without addition of the cells.
Sugimori
Daisuke
D
Department of Industrial System, Faculty of Symbiotic Systems Science, Fukushima University, 1 Kanayagawa, Fukushima 960-1296, Japan. sugimori@sss.fukushima-u.ac.jp
D001673:Biodegradation, Environmental; D018920:Coculture Techniques; D003454:Cryptococcus / Q000302:isolation & purification / Q000378:metabolism*; D009821:Oils / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D012248:Rhodotorula / Q000302:isolation & purification / Q000378:metabolism*
D009821:Oils; D010938:Plant Oils