Highly homologous hS100A15 and hS100A7 proteins are distinctly expressed in normal breast tissue and breast cancer.
Human S100A7 (psoriasin) is considered a marker for specific stages of breast cancer. hS100A15 is almost identical to hS100A7 and difficult to discriminate. We developed specific probes to distinguish hS100A7 and hS100A15, and demonstrate their differential distribution in normal breast tissue. Further, hS100A7 and S100A15 transcripts are elevated in ER/PR negative breast cancers, but hS100A15 protein is detected in all cancer specimens while hS100A7 protein is sporadically expressed. The differential regulation, expression and distribution of hS100A7 and hS100A15 and their reported distinct functions are compelling reasons to discriminate among these proteins in normal breast and breast cancers.
Wolf
Ronald
R
Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD 20892-4255, USA.
Voscopoulos
Christopher
C
Winston
Jason
J
Dharamsi
Alif
A
Goldsmith
Paul
P
Gunsior
Michele
M
Vonderhaar
Barbara K
BK
Olson
Melanie
M
Watson
Peter H
PH
Yuspa
Stuart H
SH
D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000235:genetics; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000032:analysis* / Q000235:genetics
D002135:Calcium-Binding Proteins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; C510475:S100A7A protein, human