IFN-gamma down-regulates Secretoglobin 3A1 gene expression.
STAT1 mediates Interferon (IFN)-dependent positive and negative regulation of inflammatory gene expression in lung. In this study, we examined the effect of IFN-gamma on the expression of SCGB3A1 which is thought to play crucial roles in inflammation and epithelial cell differentiation in lung. We found that expression of SCGB3A1 was down-regulated by IFN-gamma in a time- and dose-dependent manner in the murine transformed Clara Cells (mtCC) line. IFN-gamma induced the phosphorylation of STAT1, which binds to a STAT-binding element (SBE) in the SCGB3A1 gene promoter, leading to decreased transcriptional activation of this gene.
Yamada
Atsushi
A
Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan.
Suzuki
Dai
D
Miyazono
Agasa
A
Oshima
Kumiko
K
Kamiya
Akihide
A
Zhao
Baohong
B
Takami
Masamichi
M
Donnelly
Raymond P
RP
Itabe
Hiroyuki
H
Yamamoto
Matsuo
M
Kimura
Shioko
S
Kamijo
Ryutaro
R
D000818:Animals; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D017471:Receptors, Interferon / Q000378:metabolism; D011994:Recombinant Proteins; D050794:STAT1 Transcription Factor / Q000378:metabolism; D000096743:Interferon gamma Receptor
D007371:Interferon-gamma; D011506:Proteins; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D050794:STAT1 Transcription Factor; D000096743:Interferon gamma Receptor; C494080:Stat1 protein, mouse; C470307:UGRP2 protein, mouse