Germinated Buckwheat extract decreases blood pressure and nitrotyrosine immunoreactivity in aortic endothelial cells in spontaneously hypertensive rats.
Thee present study analysed the quantification of rutin in raw buckwheat extract (RBE) and germinated buckwheat extract (GBE) by high performance liquid chromatography (HPLC), and examined changes in body weight, systolic blood pressure (SBP) and nitrotyrosine (a marker for peroxynitrite formation) immunoreactivity in aortic endothelial cells in spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats after treatment with RBE and GBE for 5 weeks. In the HPLC study, RBE and GBE contained a mean content of rutin of 1.52 +/- 0.21 and 2.92 +/- 0.88 mg/g, respectively. In the 600 mg/kg GBE-treated group, SBP was lower than that in the 600 mg/kg RBE-treated group. The treatment with RBE and/or GBE significantly reduced oxidative damage in aortic endothelial cells by lowering nitrotyrosine immunoreactivity. These results suggest that GBE has an antihypertensive effect and may protect arterial endothelial cells from oxidative stress.
Kim
Dae Won
DW
Central Research Institute, Natural F&P Co. Ltd, Chuncheon 200-163, South Korea. mhwon@hallym.ac.kr
Hwang
In Koo
IK
Lim
Soon Sung
SS
Yoo
Ki-Yeon
KY
Li
Hua
H
Kim
Young Sup
YS
Kwon
Dae Young
DY
Moon
Won Kuk
WK
Kim
Dong-Woo
DW
Won
Moo-Ho
MH
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001011:Aorta / Q000166:cytology; D001794:Blood Pressure / Q000187:drug effects*; D001835:Body Weight; D002851:Chromatography, High Pressure Liquid; D042783:Endothelial Cells / Q000378:metabolism; D019612:Fagopyrum / Q000737:chemistry; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012431:Rutin / Q000494:pharmacology*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D000959:Antihypertensive Agents; D010936:Plant Extracts; C002744:3-nitrotyrosine; D014443:Tyrosine; D012431:Rutin