title: Intestinal uptake of amyloid beta protein through columnar epithelial cells in suckling mice.
abstract: The mechanism of transmission of amyloid protein, especially the dynamics in the intestine, is still largely unknown. In the present study, a fusion protein (Abeta-EGFP) that combined enhanced green fluorescent protein with amyloid-beta protein (Abeta) was orally administered to mice before and after weaning, and the uptake and kinetics of amyloid protein within the intestine were elucidated through histopathology. Abeta-EGFP was incorporated into the cytoplasm of columnar epithelial cells, rather than M cells, at 3 h after administration and thereafter. Abeta-EGFP then accumulated in the crypt, Peyer's patch, and even the spleen. However, this uptake was not observed in weaned mice. These results suggest that a specific tolerant mechanism for incorporation of Abeta escaped from the digestion exists during suckling periods. This age-dependent uptake is important for estimating the risk of transmission.
authors.affiliation: Department of Molecular Immunology, Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Ano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Akikazu
authors.initials: A
authors.lastname: Sakudo
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Sawano
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Itohara
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
mesh_terms: D000284:Administration, Oral; D016229:Amyloid beta-Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000833:Animals, Suckling; D004848:Epithelium / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D008871:Microvilli / Q000378:metabolism; D008954:Models, Biological; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D016229:Amyloid beta-Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins