Determination of indole-3-acetic acid in soil using excitation-emission matrix fluorescence with trilinear decomposition-based calibration methods.
Indole-3-acetic acid (IAA) is a phytohormone of the auxin group and is capable of coordinating the overall processes of plant growth and development. IAA is active in the very low concentration range. Therefore, it is important to quantify IAA in the low concentration range in complex system. In this work, a new spectrofluorometric method for the direct determination of IAA in soil is proposed and discussed. It combines the fluorescence excitation-emission matrices (EEMs) with second-order calibration methods based on the alternating trilinear decomposition (ATLD) algorithm and the self-weighed alternating trilinear decomposition (SWATLD) algorithm. These methodologies fully exploit the second-order advantage of the three-way fluorescence data, allowing the analyte concentrations to be quantified even in the presence of unknown fluorescent interferents. IAA recoveries in soil were determined as 100.6 +/- 3.0 and 96.9 +/- 1.1% with ATLD and SWATLD, respectively. The limits of detection obtained were 17.6 and 4.6 ng mL(-1), and the limits of quantification were 52.9 and 13.9 ng mL(-1) with ATLD and SWATLD, respectively.
Li
Yuan-Na
YN
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Wu
Hai-Long
HL
Zhu
Shao-Hua
SH
Nie
Jin-Fang
JF
Yu
Yong-Jie
YJ
Wang
Xue-Mei
XM
Yu
Ru-Qin
RQ
D002138:Calibration; D007210:Indoleacetic Acids / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis; D012987:Soil / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000592:standards
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D012987:Soil; C030737:indoleacetic acid