title: FMRFamide-like neuropeptides as putative ligands of the latrophilin-like HC110-R from Haemonchus contortus.
abstract: The latrophilin-like receptor HC110-R of the parasitic nematode Haemonchus contortus has been previously identified as a target for the novel anthelmintic drug emodepside, but the natural ligand(s) remained completely unknown to date. Here, we investigate 11 different FMRFamide-like neuropeptides as putative ligands by surface plasmon resonance with an immobilized recombinant 54kDa aminoterminal fragment of HC110-R as an interaction partner. AF1, AF10 and PF2 exhibit binding with low affinities as indicated by a K(d) of 11 microM for AF1, 52 microM for AF10 and 583 microM for PF2. Our data indicate that AF1, AF10, and PF2 are putative natural ligands of HC110-R presumably involved in the control of pharyngeal pumping of nematode worms.
authors.affiliation: Division of Molecular Parasitology and Centre of Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, Düsseldorf, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Mühlfeld
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Schmitt-Wrede
authors.forename: Achim
authors.initials: A
authors.lastname: Harder
authors.forename: Frank
authors.initials: F
authors.lastname: Wunderlich
mesh_terms: D000818:Animals; D019835:FMRFamide / Q000378:metabolism*; D006189:Haemonchus / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: C427647:HC110-R protein, Haemonchus contortus; D008565:Membrane Proteins; D009479:Neuropeptides; D019835:FMRFamide