title: Phagocytosis, oxidative burst, and produced reactive species are affected by iron deficiency anemia and anemia of chronic diseases in elderly.
abstract: Iron and oxidative stress have a regulatory interplay. During the oxidative burst, phagocytic cells produce free radicals such as hypochlorous acid (HOCl). Nevertheless, scarce studies evaluated the effect of either iron deficiency anemia (IDA) or anemia of chronic disease (ACD) on phagocyte function in the elderly. The aim of the present study was to determine the oxidative burst, phagocytosis, and nitric oxide (*NO) and HOCl, reactive species produced by monocytes and neutrophils in elderly with ACD or IDA. Soluble transferrin receptor, serum ferritin, and soluble transferrin receptor/log ferritin (TfR-F) index determined the iron status. The study was constituted of 39 patients aged over 60 (28 women and 11 men) recruited from the Brazilian Public Health System. Oxidative burst fluorescence intensity per neutrophil in IDA group and HOCl generation in both ACD and IDA groups were found to be lower (p < 0.05). The percentages of neutrophils and monocytes expressing phagocytosis in ACD group were found to be higher (p < 0.05). There was an overproduction of *NO from monocytes, whereas the fundamental generation of HOCl appeared to be lower. Phagocytosis, oxidative burst, and *NO and HOCl production are involved in iron metabolism regulation in elderly patients with ACD and IDA.
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Monte Alegre, Ribeirão Preto, São Paulo, Brazil.
authors.forename: I M M
authors.initials: IM
authors.lastname: Paino
authors.forename: J C
authors.initials: JC
authors.lastname: Miranda
authors.forename: C M
authors.initials: CM
authors.lastname: Marzocchi-Machado
authors.forename: E J
authors.initials: EJ
authors.lastname: Cesarino
authors.forename: F A
authors.initials: FA
authors.lastname: de Castro
authors.forename: A M
authors.initials: AM
authors.lastname: de Souza
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000740:Anemia / Q000378:metabolism*; D018798:Anemia, Iron-Deficiency / Q000378:metabolism; D002478:Cells, Cultured; D002908:Chronic Disease; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D006997:Hypochlorous Acid