title: Genetically timed, activity-sensor and rainbow transsynaptic viral tools.
abstract: We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged, H-6720, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Boldogkoi
authors.forename: Kamill
authors.initials: K
authors.lastname: Balint
authors.forename: Gautam B
authors.initials: GB
authors.lastname: Awatramani
authors.forename: David
authors.initials: D
authors.lastname: Balya
authors.forename: Volker
authors.initials: V
authors.lastname: Busskamp
authors.forename: Tim James
authors.initials: TJ
authors.lastname: Viney
authors.forename: Pamela S
authors.initials: PS
authors.lastname: Lagali
authors.forename: Jens
authors.initials: J
authors.lastname: Duebel
authors.forename: Emese
authors.initials: E
authors.lastname: Pásti
authors.forename: Dóra
authors.initials: D
authors.lastname: Tombácz
authors.forename: Judit S
authors.initials: JS
authors.lastname: Tóth
authors.forename: Irma F
authors.initials: IF
authors.lastname: Takács
authors.forename: Brigitte Gross
authors.initials: BG
authors.lastname: Scherf
authors.forename: Botond
authors.initials: B
authors.lastname: Roska
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D001921:Brain / Q000166:cytology / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009474:Neurons / Q000502:physiology / Q000821:virology; D009435:Synaptic Transmission / Q000502:physiology*; D013997:Time Factors; D014795:Visual Pathways / Q000502:physiology / Q000821:virology*; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein