User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.
We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.
Schroeder
Hendrik
H
Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.
Adler
Michael
M
Gerigk
Katrin
K
Müller-Chorus
Birgit
B
Götz
Friedrich
F
Niemeyer
Christof M
CM
D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D040081:Protein Array Analysis
D015415:Biomarkers; D014408:Biomarkers, Tumor; D004247:DNA