[An experimental study on effect of implanting bFGF into denervated skeletal muscle to muscle satellite cell proliferation and muscle atrophy].
OBJECTIVE
To investigate the effect of bFGF on denervated skeletal muscle in accelerating muscle satellite cell proliferation, supplying neurotrophic factors and reducing muscle atrophy.


METHODS
Twenty-eight Wistar male rats were divided into the experimental group and the control group randomly, whose left lower limb sciatic nerve was excised to make animal models of denervated skeletal muscle. The silia gel tubes containing 0.1 g bFGF and normal saline were implanted into gastrocnemius in the experimental and control groups, respectively. After 14 and 30 days of operation, gross appearance was observed; muscle wet weight and potential amplitude of gastrocnemius fibrillation were measured; histological observation and electron microscope observation were made.


RESULTS
At 14 and 30 days after operation, gastrocnemius atrophy and adhesion were more obvious in the control group than those in the experimental group. At 30 days after operation, the potential amplitude of gastrocnemius fibrillation and muscle wet weight were experimental group (0.220 6 +/- 0.301 0) microm and (2.475 7 +/- 0.254 6) g in the experimental group, and (0.155 2 +/- 0.050 3) microm and (1.459 1 +/- 0.642 5) g in the control group. There was a significant difference between two groups (P < 0.05). At 14 and 30 days after operation, HE staining showed more muscle satellite cell nuclei in gastrocnemius of the experimental group than that of the control group; Mallory staining showed more blue connective tissues in the control group than in the experimental group; PCNA staining showed more PCNA positive cell nuclei in the experimental group than in the control group; and the AgNO3 staining testified more grains of vitamin C and less connective tissue proliferation in the experimental group than in the control group. At 30 days after operation, the fiber diameter and the fiber area were (66.368 6 +/- 12.672 7) microm and (2 096.112 9 +/- 311.563 9) microm2 in the experimental group, (55.5040 +/- 4.945 0) microm and (1 418.0680 +/- 264.953 7) microm2 in the control group. The PCNA positive cell nuclei number was 116.200 +/- 5.357 in the experimental group and 53.000 +/- 3.937 in the control group, showing statistically significant difference between the two groups (P < 0.05). At 14 and 30 days after operation, compared with control group, the muscle fiber in the experimental group arrangedly more regularly and had less atrophy fiber and the connective tissue proliferation.


CONCLUSIONS
bFGF can stimulate the proliferation of muscle satellite cells in denervated gastrocnemius, delay the muscle fiber atrophy and inhibit connective tissues proliferation in muscle fibers.
Xu
Hui
H
Department of Hand Surgery, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang Liaoning 110024, PR China. letibe1120@yahoo.com.cn
Zhu
Yue
Y
Xin
Changtai
C
D000818:Animals; D049109:Cell Proliferation; D008297:Male; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000294:innervation / Q000601:surgery*; D009414:Nerve Growth Factors; D051381:Rats; D017208:Rats, Wistar; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*
D009414:Nerve Growth Factors