[Denaturing high-performance liquid chromatography for identifying four categories of diarrheagenic Escherichia coli].
OBJECTIVE
We report a new molecular technique for the analysis of four categories of diarrheagenic Escherichia coli based on the separation of PCR-amplified target fragments by denaturing high-performance liquid chromatography (DHPLC).


METHODS
Enteropathogenic E.coli, enterotoxigenic E.coli, enterohemorrhagic E.coli and enteroinvasive E.coli were identified by analyzing four specific virulence genes. RESULTS; A total of 32 bacterial strains were tested, giving no false-positive or false negative results. The detection limits were as low as: ETEC 27 CFU/mL, EPEC 33 CFU/mL, EHEC 25 CFU/mL and EIEC 42 CFU/mL.


CONCLUSIONS
The data suggest that PCR-DHPLC will be useful for diarrheagenic Escherichia coli detection and identification.
Xu
Junyi
J
Liaoning Entry-Exit Inspection and Quarantine Bureau of China, Dalian 116001, China. skyxjy@yahoo.com
Cao
Jijuan
J
Zheng
Qiuyue
Q
Zhao
Xin
X
Yan
Pingping
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction