title: Cost-effective diffuse reflectance spectroscopy device for quantifying tissue absorption and scattering in vivo.
abstract: A hybrid optical device that uses a multimode fiber coupled to a tunable light source for illumination and a 2.4-mm photodiode for detection in contact with the tissue surface is developed as a first step toward our goal of developing a cost-effective, miniature spectral imaging device to map tissue optical properties in vivo. This device coupled with an inverse Monte Carlo model of reflectance is demonstrated to accurately quantify tissue absorption and scattering in tissue-like turbid synthetic phantoms with a wide range of optical properties. The overall errors for quantifying the absorption and scattering coefficients are 6.0+/-5.6 and 6.1+/-4.7%, respectively. Compared with fiber-based detection, having the detector right at the tissue surface can significantly improve light collection efficiency, thus reducing the requirement for sophisticated detectors with high sensitivity, and this design can be easily expanded into a quantitative spectral imaging system for mapping tissue optical properties in vivo.
authors.affiliation: Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708-0281, USA. bing.yu@duke.edu
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.forename: Justin Y
authors.initials: JY
authors.lastname: Lo
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Kuech
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Palmer
authors.forename: Janelle E
authors.initials: JE
authors.lastname: Bender
authors.forename: Nirmala
authors.initials: N
authors.lastname: Ramanujam
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008029:Lighting / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D009391:Nephelometry and Turbidimetry / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D019047:Phantoms, Imaging; D010783:Photometry / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D011874:Radiometry / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D014159:Transducers / Q000191:economics; D014481:United States