Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine.
For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides containing 8-thioxodeoxyadenosine (s(8)dA) residues in place of the protonated deoxycytidines required for the third base pairing with DNA duplexes. Consequently, it was found that s(8)dA exhibited much stronger hybridization ability than dC under neutral conditions when four s(8)dA bases were arranged in a consecutive sequence.
Miyata
Ken-ichi
K
Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan.
Tamamushi
Ryuji
R
Tsunoda
Hirosuke
H
Ohkubo
Akihiro
A
Seio
Kohji
K
Sekine
Mitsuo
M
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009946:Organothiophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C535198:6-N-carbamoyl-8-thioxodeoxyadenosine 3'-phosphoramidite; C535199:8-thioxodeoxyadenosine; D003839:Deoxyadenosines; D009838:Oligodeoxyribonucleotides; D009946:Organothiophosphorus Compounds; D004247:DNA