title: [Effect of suppressive oligodeoxynucleotides on the functional differentiation in CD4(+) Th1 lymphocytes in mice in vitro].
abstract: OBJECTIVE
  To explore the effect of suppressive oligodeoxynucleotides (Sup ODN) on the Th1 differentiation of CD4(+)T splenetic lymphocytes in mice.
  
  
  METHODS
  The splenetic lymphocytes of BALB/c mice were separated, and then CD4(+) cells were purified with immune magnetic CD4(+) microbeads (positive selection). The purification was examined by fluorescence-activated cell sorter. CD4(+) cells, anti-CD3epsilon, anti-CD28, IL-12 and Sup ODN or control oligodeoxynucleotides (Con ODN) were co-incubated for 72 h. IFN-gamma and IL-4 in the supernatant were detected using enzyme-linked immunosorbent assay. The expression of T-bet mRNA in CD4(+) cells was tested by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Sup ODN could significantly inhibit the release of INF-gamma and increase IL-4 production respectively (P<0.01). T-bet mRNA of CD4(+) lymphocytes was remarkably inhibited by Sup ODN as well (P<0.01).
  
  
  CONCLUSIONS
  In the presence of pro-Th1-cytokines, Sup ODN may affect the differentiation of CD4(+) T lymphocytes in vitro. Sup ODN can promote CD4(+) T cells to differentiate into Th2, and suppress them into Th1.
authors.affiliation: Department of Infection , Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Sha-Ling
authors.initials: SL
authors.lastname: Li
authors.forename: Xia-Hong
authors.initials: XH
authors.lastname: Dai
authors.forename: Xue-Gong
authors.initials: XG
authors.lastname: Fan
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000502:physiology; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology*
chemical_list: D009838:Oligodeoxyribonucleotides; D018664:Interleukin-12; D007371:Interferon-gamma