[Up-to-date technologies of laboratory diagnosis of tuberculosis (efficiency of their use in clinical practice)].
To explore the diagnostic informative value of using new diagnostic methods for tuberculosis, the authors made a comparative study of the use of real-time polymerase chain reaction (PCR) and enzyme immunoassay (EIA) to determine Mycobacterium tuberculosis antibodies in 96 patients diagnosed at the Saratov regional tuberculosis dispensary. Real-time PCR was found to be highly effective in improving the quality of diagnosis of tuberculosis and its differential diagnosis from another lung abnormality, by increasing the prognostic value of a positive result in PCR up to 95.2% versus 85.3% in EIA (p=0.0220) and that of a negative result to 77.7% versus 62.9%, respectively (p=0.0238).
Salina
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D000293:Adolescent; D000328:Adult; D000368:Aged; D019411:Clinical Laboratory Techniques / Q000639:trends*; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D003948:Diagnostic Techniques, Respiratory System / Q000639:trends*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000639:trends; D015203:Reproducibility of Results; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*; D055815:Young Adult
D004269:DNA, Bacterial