Oxidative DNA damage in retinopathy of prematurity.
OBJECTIVE
This study examines the levels of oxidative damage in patients with retinopathy of prematurity (ROP).


METHODS
Fifty patients were recruited with a birthweight below 1500 g or gestational age below 32 weeks. The cases were classified into those who developed ROP (n=25) and those without ROP (n=25). The authors obtained blood and urine samples from each infant, for measuring 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels, at the time of the first examination at 4-6 postnatal weeks.


RESULTS
A significant difference was observed in leukocyte and urine 8-OHdG levels in patients with ROP compared to those without ROP (p<0.001 for both). Similarly, a significant difference was observed in plasma and urine MDA levels in patients with ROP compared to those without ROP (p<0.001 for both). In addition, significant correlations were found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r=0.859, p<0.001), and between levels of urine 8-OHdG excretion and urine MDA (r=0.563, p<0.001).


CONCLUSIONS
8-OHdG in leukocyte DNA and urine levels in premature infants can be useful as an indicator for ROP screening.
Ates
O
O
Department of Ophthalmology Medical Faculty, Ataturk University, Erzurum, Turkey. orhanates@atauni.edu.tr
Alp
H H
HH
Caner
I
I
Yildirim
A
A
Tastekin
A
A
Kocer
I
I
Baykal
O
O
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001724:Birth Weight; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005865:Gestational Age; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D007962:Leukocytes / Q000378:metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood / Q000652:urine; D015997:Neonatal Screening / Q000379:methods; D018384:Oxidative Stress; D012178:Retinopathy of Prematurity / Q000175:diagnosis*
D015415:Biomarkers; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine