title: Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae mixed fermented feed on the enhanced growth performance of broilers.
abstract: Bacillus subtilis var. natto N21 (Bac; for greater proteolytic capacity) and Saccharomyces cerevisiae Y10 (Sac; for greater acidic capacity) were applied to produce a 2-stage combined fermentation feed. This study investigated whether the enhancement of Bac+Sac fermented feed on broiler growth performance was due to the probiotics per se or due to the fermentation process. Trial 1 included 1-d-old broiler chicks (n=144) randomly assigned to control, water added (same as in the fermentation feed, 23%), and Bac+Sac fermented feed (FBac+Sac) treatments with 4 replicates. Trial 2 included 21-d-old broiler chickens (n=12) assigned into control and FBac+Sac groups for a metabolic trial for nutrient availability. Trial 3 included 1-d-old male broiler chicks (n=216) randomly assigned into 6 treatments with 3 replicates. Treatments included a control, Sac fermented feed (FSac), FBac+Sac, Bac powder (PBac), Sac powder (PSac), and Bac+Sac powder (PBac+Sac). The results from trial 1 showed that FBac+Sac increased BW and feed intake (P<0.05) in 21- and 39-d-old chickens. The water-added group showed decreased BW, weight gain, and feed intake (P<0.05). Trial 2 showed that FBac+ Sac increased gross energy availability (P<0.05). Trial 3 showed that FBac+Sac increased 21- and 39-d-old BW and weight gain (P<0.05). Diets supplemented with probiotic powder or fermented with Sac did not improve broiler growth performance (P>0.05). The growth performance improvement of the FBac+Sac treatment was probably not due to the added water, probiotic powder inclusion, or through single-strain fermentation, but due to the 2-stage fermentation process using Bac and Sac strains.
authors.affiliation: Department of Animal Science, National Chiayi University, Taiwan.
authors.forename: K-L
authors.initials: KL
authors.lastname: Chen
authors.forename: W-L
authors.initials: WL
authors.lastname: Kho
authors.forename: S-H
authors.initials: SH
authors.lastname: You
authors.forename: R-H
authors.initials: RH
authors.lastname: Yeh
authors.forename: S-W
authors.initials: SW
authors.lastname: Tang
authors.forename: C-W
authors.initials: CW
authors.lastname: Hsieh
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001412:Bacillus subtilis; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism; D004032:Diet / Q000662:veterinary*; D005285:Fermentation; D005766:Gastrointestinal Contents / Q000737:chemistry / Q000382:microbiology; D007422:Intestines / Q000166:cytology / Q000201:enzymology; D008297:Male; D011897:Random Allocation; D012441:Saccharomyces cerevisiae