title: Standardization of retention time data for AMT tag proteomics database generation.
abstract: The combination of liquid chromatography (LC) with mass spectrometry (MS) has become a mainstream proteome analysis strategy. In LC-MS, measured masses possess their "universal" scale derived from atomic mass tables. In contrast, the observed LC retention times (RT) are not tied to a conventional time scale, and depend on experimental conditions. However, RT data, being explicitly orthogonal to MS, offer relevant information for proteome characterization. We present here a strategy for peptides RT data standardization, based on the generation of a standard scale using retention prediction models, which enables sharing of identification databases in the context of multi-laboratory research.
authors.affiliation: Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Tarasova
authors.forename: V
authors.initials: V
authors.lastname: Guryca
authors.forename: M L
authors.initials: ML
authors.lastname: Pridatchenko
authors.forename: A V
authors.initials: AV
authors.lastname: Gorshkov
authors.forename: S
authors.initials: S
authors.lastname: Kieffer-Jaquinod
authors.forename: V V
authors.initials: VV
authors.lastname: Evreinov
authors.forename: C D
authors.initials: CD
authors.lastname: Masselon
authors.forename: M V
authors.initials: MV
authors.lastname: Gorshkov
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030562:Databases, Protein; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry; D040901:Proteomics
chemical_list: D011506:Proteins