Mass spectrometric profile of the serum as a marker of experimental psychoemotional stress in rats.
Mass spectrometric profiling of blood serum from stressed and control rats was used for the detection of stress marker proteins. Profiling with reversed-phase chips revealed 6 discriminatory mass spectrometry peaks. Cluster analysis showed that the animals can be divided into 2 groups by the intensity of these peaks. One group mainly consists of stressed rats, while other includes control specimens. Profiling of serum samples after fractionation on reversed-phase magnetic granules allowed us to identify an 8910-Da biomarker. Our results indicate that mass spectrometric profiling holds much promise for the detection of stress markers in the serum.
Koplik
E V
EV
P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia.
Vlasova
M A
MA
Moshkovsky
S A
SA
Archakov
A I
AI
Sudakov
K V
KV
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism; D008297:Male; D040081:Protein Array Analysis; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013315:Stress, Psychological / Q000097:blood*
D015415:Biomarkers; D001798:Blood Proteins