title: Abnormal mitochondrial function in locomotor and respiratory muscles of COPD patients.
abstract: Several cellular and molecular alterations have been described in skeletal and respiratory muscles of patients with chronic obstructive pulmonary disease (COPD), but information on potential abnormalities of mitochondrial function is scarce. The aim of the present study was to investigate mitochondrial function in the vastus lateralis (VL) and external intercostalis (EI) of COPD patients. Biopsies from VL and EI were obtained during surgery for lung cancer in 13 patients with mild to moderate COPD (age 68+/-6 yrs, forced expiratory volume in one second (FEV(1)) 66+/-15% predicted) and 19 control subjects (age 67+/-9 yrs, FEV(1) 95+/-18% pred). State 3 and 4 mitochondrial oxygen consumption (V'(O(2),m)), ATP synthesis, citrate synthase, cytochrome oxidase (COX) and complex I-III activities, as well as reactive oxygen species (ROS) production, were determined. In COPD patients, in both muscles, COX activity (VL: COPD 3.0+/-0.8 versus control 2.0+/-0.8; EI: 3.7+/-1.6 versus 2.4+/-0.9 micromol min(-1) mg(-1)) and ROS production (VL: 1,643+/-290 versus 1,285+/-468; EI: 1,033+/-210 versus 848+/-288 arbitrary units) were increased, whereas state 3 V'(O(2),m) was reduced (VL: 2.9+/-0.3 versus 3.6+/-0.4; EI: 3.6+/-0.3 versus 4.1+/-0.4 mmol min(-1) kg(-1)). Skeletal muscle mitochondria of patients with chronic obstructive pulmonary disease show electron transport chain blockade and excessive production of reactive oxygen species. The concurrent involvement of both vastus lateralis and external intercostalis suggests a systemic (rather than a local) mechanism(s) already occurring in relatively early stages (Global Initiative for Chronic Obstructive Lung Disease stage II) of the disease.
authors.affiliation: Servicio de Neumología, Hospital General Universitario Gregorio Marañón, Madrid, Spain. lpuente@separ.es
authors.forename: L
authors.initials: L
authors.lastname: Puente-Maestu
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Parra
authors.forename: R
authors.initials: R
authors.lastname: Godoy
authors.forename: N
authors.initials: N
authors.lastname: Moreno
authors.forename: A
authors.initials: A
authors.lastname: Tejedor
authors.forename: F
authors.initials: F
authors.lastname: González-Aragoneses
authors.forename: J-L
authors.initials: JL
authors.lastname: Bravo
authors.forename: F Villar
authors.initials: FV
authors.lastname: Alvarez
authors.forename: S
authors.initials: S
authors.lastname: Camaño
authors.forename: A
authors.initials: A
authors.lastname: Agustí
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000368:Aged; D001706:Biopsy; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000503:physiopathology*; D010101:Oxygen Consumption / Q000502:physiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism / Q000503:physiopathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012132:Respiratory Muscles / Q000378:metabolism / Q000503:physiopathology*; D013147:Spirometry; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D013384:Succinate Cytochrome c Oxidoreductase; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase