KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation.
A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library. Computational analysis showed that KCTD10 cDNA is 2,638 bp long, encoding 313 amino acids with a proliferating cell nuclear antigen binding motif, mapped to chromosome 12q24.11 with 7 exons, ubiquitously expressed in all 12 tested normal tissues and 7 of 8 tested tumor cell lines from MTN membranes by Northern blot. Nuclear localization of KCTD10 was observed in A549 cells. Yeast two-hybrid analysis and immunoprecipitation assay showed that KCTD10 can interact with PCNA. In A549 cells, KCTD10 down-regulation could inhibit cell proliferation, but its over-expression could not influence cell proliferation. The results suggest that KCTD10 may be associated with DNA synthesis and cell proliferation.
Wang
Yibo
Y
Key Laboratory for Clinical Cardiovascular Genetics, Ministry of Education, China & Sino-German Laboratory for Molecular Medicine, FuWai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Zheng
Yi
Y
Luo
Fang
F
Fan
Xiaohan
X
Chen
Jinxing
J
Zhang
Channa
C
Hui
Rutai
R
D021581:Active Transport, Cell Nucleus; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D009928:Organ Specificity; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics
C539187:KCTD10 protein, human; D024642:Potassium Channels, Voltage-Gated; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger