The use of components of plant origin in the development of production technology for live cold-adapted cultural influenza vaccine.
The impact of culturing conditions (multiplicity of cell culture infection with influenza virus, composition of growth and maintenance nutrient media) for the efficiency of multiplication of cold-adapted reassortant vaccine strains of influenza A and B viruses was evaluated. Soybean hydrolysate protein-based biological additive to nutrient medium provided effective reproduction of influenza A virus in MDCK cells in the presence of 2 microg/ml trypsin. The use of soybean peptone-based stabilizer provided retention of infectious titer of influenza virus grown in MDCK culture after its lyophilization to a level of 8.5 lg EID50/ml.
Mazurkova
N A
NA
Vector Center of Virology and Biotechnology, Koltsovo, Novosibirsk region, Russia. mazurkova@vector.nsc.ru
Kolokol'tsova
T D
TD
Nechaeva
E A
EA
Shishkina
L N
LN
Sergeev
A N
AN
D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D004285:Dogs; D005260:Female; D006801:Humans; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000517:prevention & control*; D009975:Orthomyxoviridae / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry; D014779:Virus Replication
D007252:Influenza Vaccines; D010940:Plant Proteins