title: Heterostructured ZnO/Au nanoparticles-based resonant Raman scattering for protein detection.
abstract: A new method of protein detection was explored on the resonant Raman scattering signal of ZnO nanoparticles. A probe for the target protein was constructed by binding the ZnO/Au nanoparticles to secondary protein by eletrostatic interaction. The detection of proteins was achieved by an antibody-based sandwich assay. A first antibody, which could be specifically recognized by target protein, was attached to a solid silicon surface. The ZnO/Au protein probe could specifically recognize and bind to the complex of the target protein and first antibody. This method on the resonant Raman scattering signal of ZnO nanoparticles showed good selectivity and sensitivity for the target protein.
authors.affiliation: Center for Advanced Optoelectronic Functional Materials Research, Northeast Normal University, Changchun 130024, P. R. China.
authors.forename: Guiye
authors.initials: G
authors.lastname: Shan
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Fei
authors.forename: Yichun
authors.initials: Y
authors.lastname: Liu
authors.forename: Guoliang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D013059:Spectrum Analysis, Raman / Q000379:methods*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D006046:Gold; D015034:Zinc Oxide