title: [Progress of antisense technology applied in metabolic regulation of bacteria].
abstract: With the rapid development of genetic engineering and metabolic regulation, antisense technology displays its fascination to the world as a mild regulation genetic tool. Compared with other loss-of-function research methods (e.g. gene knockout), antisense technologies have advantages such as low cost, short period, and easy operation. It has been increasingly used in bacterial metabolic regulation as a powerful genetic tool. This review briefly summarized the latest progress and problems in antisense technologies that are recently used in metabolic engineering of bacteria, and compares the advantages and disadvantages of these technologies.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Jianming
authors.initials: J
authors.lastname: Yang
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Nie
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005818:Genetic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics*
chemical_list: D016376:Oligonucleotides, Antisense; D016372:RNA, Antisense; D016337:RNA, Catalytic