title: Neuronal release of proBDNF.
abstract: Pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF utilize distinct receptors to mediate divergent neuronal actions. Using new tools to quantitate endogenous BDNF isoforms, we found that mouse neurons secrete both proBDNF and mature BDNF. The highest levels of proBDNF and p75 were observed perinatally and declined, but were still detectable, in adulthood. Thus, BDNF actions are developmentally regulated by secretion of proBDNF or mature BDNF and by local expression of p75 and TrkB.
authors.affiliation: Department of Medicine, Weill Cornell Medical College, 1300 York Ave., New York, New York 10065, USA.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yang
authors.forename: Chia-Jen
authors.initials: CJ
authors.lastname: Siao
authors.forename: Guhan
authors.initials: G
authors.lastname: Nagappan
authors.forename: Tina
authors.initials: T
authors.lastname: Marinic
authors.forename: Deqiang
authors.initials: D
authors.lastname: Jing
authors.forename: Kelly
authors.initials: K
authors.lastname: McGrath
authors.forename: Zhe-Yu
authors.initials: ZY
authors.lastname: Chen
authors.forename: Willie
authors.initials: W
authors.lastname: Mark
authors.forename: Lino
authors.initials: L
authors.lastname: Tessarollo
authors.forename: Francis S
authors.initials: FS
authors.lastname: Lee
authors.forename: Bai
authors.initials: B
authors.lastname: Lu
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Hempstead
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D055879:Gene Knock-In Techniques; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D008817:Mice, Mutant Strains; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor; D011498:Protein Precursors; D020800:Receptor, Nerve Growth Factor; C426674:brain-derived neurotrophic factor precursor; D020813:Receptor, trkB