title: Vitrification of corneal endothelial cells in a monolayer.
abstract: An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Fan
authors.forename: Xue-Hu
authors.initials: XH
authors.lastname: Ma
authors.forename: Tian-Qing
authors.initials: TQ
authors.lastname: Liu
authors.forename: Zhan-Feng
authors.initials: ZF
authors.lastname: Cui
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004728:Endothelium, Corneal / Q000166:cytology*; D019946:Propylene Glycol / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*
chemical_list: D003470:Culture Media; D019946:Propylene Glycol; D014199:Trehalose