Glycomic characterization of prostate-specific antigen and prostatic acid phosphatase in prostate cancer and benign disease seminal plasma fluids.
Prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP) are glycoproteins secreted by prostate epithelial cells, and have a long clinical history of use as serum biomarkers of prostate cancers. These two proteins are present at significantly higher concentrations in seminal plasma, making this proximal fluid of the prostate a good source for purifying enough protein for characterization of prostate disease associated changes in glycan structures. With the use of seminal fluid samples representative of normal control, benign prostatic disease and prostate cancers, PAP and PSA were enriched by thiophilic absorption chromatography. Released N-linked glycan constituents from both proteins were analyzed by a combination of normal phase HPLC and MALDI-TOF spectrometry. For PSA, 40 putative glycoforms were determined, and 21 glycoforms were determined for PAP. PAP glycans were further analyzed with a hybrid triple quadrupole/linear ion trap mass spectrometer to assign specific glycoform classes to each of the three N-linked sites. The glycans identified in these studies will allow for more defined targeting of prostate disease-specific changes for PAP, PSA and other secreted prostatic glycoproteins.
White
Krista Y
KY
Department of Microbiology, Eastern Virginia Medical School, Norfolk, Virginia 23507, USA.
Rodemich
Lucy
L
Nyalwidhe
Julius O
JO
Comunale
Mary Ann
MA
Clements
Mary Ann
MA
Lance
Raymond S
RS
Schellhammer
Paul F
PF
Mehta
Anand S
AS
Semmes
O John
OJ
Drake
Richard R
RR
D000135:Acid Phosphatase; D014408:Biomarkers, Tumor / Q000097:blood / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D054794:Glycomics / Q000379:methods*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis; D017430:Prostate-Specific Antigen / Q000097:blood / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000097:blood* / Q000737:chemistry / Q000201:enzymology; D017027:Protein Tyrosine Phosphatases / Q000097:blood / Q000737:chemistry; D012661:Semen / Q000737:chemistry / Q000201:enzymology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D011134:Polysaccharides; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases; D017430:Prostate-Specific Antigen