[Duration of preservation of viable cells, DNA, and antigens of Mycoplasma hominis and ureaplasmas in human serum at 37 degrees C].
How long the viable cells of M. hominis, Ureaplasma spp., U. urealyticum, U. parvum, and their antigens retained in human serum at 37 degrees C was investigated. M. hominis cells were shown to hold their viability within 12 days with a gradual titer drop, the antigens being also detected within 12 days whereas intracellular and extracellular DNAs were seen within 40 days (an observation time). Under the same conditions, Ureaplasma cells died after 24 hours, their antigens were disrupted following 3 days and intracellular and extracellular DNAs of different species were detectable by polymerase chain reaction (PCR) within 17-40 days. The long preservation of extracellular and dead cell DNAs suggests that diagnostic examination of patients by means of PCR may yield false-positive results.
Rakovskaia
I V
IV
Barkhatova
O I
OI
Balabanov
D N
DN
Gorina
L G
LG
Goncharova
S A
SA
Gamova
N A
NA
D000942:Antigens, Bacterial / Q000097:blood*; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000097:blood*; D006801:Humans; D050296:Microbial Viability; D019535:Mycoplasma hominis / Q000166:cytology*; D044967:Serum; D013696:Temperature; D014509:Ureaplasma / Q000166:cytology*
D000942:Antigens, Bacterial; D004269:DNA, Bacterial