[Molecules of G(2)/M phase and the phosphorylation of survivin in the carcinogenesis of oral submucosal fibrosis].
OBJECTIVE
To determine the expression of Cyclin B1, p34(cdc2) and the phosphorylation of survivin (p-survivin) in oral squamous cell carcinoma and oral submucosa fibrosis (OSF), and to discuss their possible role in carcinogenesis of OSF.


METHODS
The expression of Cyclin B1, p34(cdc2) and p-survivin were analyzed by Western blotting assay in 10 cases of normal oral mucosa epithelium, 40 cases of OSF epithelium and 42 cases of oral squamous cell carcinoma (OSCC) originated from OSF, respectively. Immunoprecipitation was used to confirm the relationship between the p34(cdc2) and survivin.


RESULTS
The expression of Cyclin B1, p34(cdc2), p-p34(cdc2) and p-survivin in OSF group were significantly higher than those in normal group (P < 0.05). The expression of these molecules showed significant different (P < 0.05) between the OSF and OSCC originated from OSF, but there was no significant difference among the early stage, the moderately advanced stage and the advanced stage of OSF. Immunoprecipitation assay confirmed the combination of p34(cdc2) and survivin.


CONCLUSIONS
The important molecules in G(2)/M phase-Cyclin B1, p34(cdc2) and p-survivin may play a key role during the mitosis and proliferation of OSF, which will be helpful in early diagnosis and therapy of carcinogenesis of OSF.
Zhou
Shang-Hui
SH
Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, China.
Li
Li-Li
LL
Jian
Xin-Chun
XC
Wang
Ying
Y
Chen
Xin-Qun
XQ
Gao
Xing
X
D000293:Adolescent; D000328:Adult; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D002455:Cell Division; D056744:Cyclin B1 / Q000378:metabolism; D005260:Female; D016195:G2 Phase; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D009914:Oral Submucous Fibrosis / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D000077022:Survivin; D055815:Young Adult
C107489:BIRC5 protein, human; C534699:CCNB1 protein, human; D056744:Cyclin B1; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin; D016203:CDC2 Protein Kinase