Transglutaminases expression in human supraspinatus tendon ruptures and in mouse tendons.
The ethiopathogenesis of rotator cuff disease remains poorly understood. Many studies advocate the importance of extra cellular matrix for the homeostasis of connective tissue. Transglutaminase enzymes family has been studied in the context of connective tissue formation and stabilisation. Here, we investigated transglutaminases expression pattern in biopsies of normal and injured supraspinatus tendons of human shoulders and in the Achilles tendons of transglutaminase 2 knock-out and wild-type mice. Our results show that different transglutaminase family members are differentially expressed in human and mouse tendons, and that transglutaminase 2 is down-regulated at mRNA and protein levels upon human supraspinatus tendon ruptures.
Oliva
Francesco
F
Department of Orthopaedic and Traumatology, University of Rome Tor Vergata, School of Medicine, Viale Oxford 81, 00133 Rome, Italy. olivafrancesco@hotmail.com
Zocchi
Loredana
L
Codispoti
Andrea
A
Candi
Eleonora
E
Celi
Monica
M
Melino
Gerry
G
Maffulli
Nicola
N
Tarantino
Umberto
U
D000368:Aged; D000818:Animals; D015536:Down-Regulation; D005260:Female; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008875:Middle Aged; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D012333:RNA, Messenger / Q000096:biosynthesis; D017006:Rotator Cuff / Q000201:enzymology* / Q000473:pathology; D000070636:Rotator Cuff Injuries; D012421:Rupture; D013708:Tendon Injuries / Q000201:enzymology* / Q000473:pathology; D011503:Transglutaminases / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins