[Knockdown and overexpression of miR-219 lead to embryonic defects in zebrafish development].
MicroRNAs (miRNAs) are important post-transcriptional modulators of gene expression, which have been implicated in many physiological and pathological processes. In this study, we found that miR-219 expressed since early segmentation stages (16s) in spinal cord and mid-, hindbrain in zebrafish using Northern blotting and whole mounts ISH technologies. Moreover, knockdown or overexpression of miR-219 led to specific embryonic defects. Furthermore, TUNEL assay showed that overexpression of miR-219 induced significant cell apoptosis in the head and tail of zebrafish. Our study suggested that miR-219 may play an important role in zebrafish embryonic development.
Zhang
Man Cang
MC
Institute of Molecular and Chemical Biology, East China Normal University, Shanghai 200062.
Lv
Yan
Y
Qi
Yan Ting
YT
Zhang
Zhi
Z
Fu
Xiao Nan
XN
Yuan
Chong Gang
CG
Lai
Li Hui
LH
D000818:Animals; D015152:Blotting, Northern; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D020287:In Situ Nick-End Labeling; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology*; D008845:Microinjections; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*
D035683:MicroRNAs