Localization of C. trachomatis Inc proteins in expression of their genes in HeLa cell culture.
Co-localization of Chlamydia trachomatis incorporation membrane proteins with cell organelles was studied in HeLa cell culture after transfection by expressing vectors carrying incA, incB, incC, incD, incE, incF, incG genes, respectively, fused with the marker green fluorescent protein (EGFP) gene. The prokaryotic proteins were co-located with compartments of the secretory pathway of the eukaryotic cell in the course of biogenesis.
Shkarupeta
M M
MM
Institute of Physico-Chemical Medicine, Russian Ministry of Health, Moscow, Russia. marnosk@yandex.ru
Kostrjukova
E S
ES
Lazarev
V N
VN
Levitskii
S A
SA
Basovskii
Yu I
YI
Govorun
V M
VM
D001426:Bacterial Proteins / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
D001426:Bacterial Proteins; C460868:IncA protein, Chlamydia trachomatis; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins