title: Important roles of glycosylphosphatidylinositol (GPI)-specific phospholipase D and some GPI-anchored proteins in the pathogenesis of hepatocellular carcinoma.
abstract: OBJECTIVE
  To investigate the roles of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in the pathogenesis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  The expression of the GPI-PLD in HCC was determined. The GPI-PLD gene was stably transfected in HepG2 cells and the proliferation of these cells was detected; CD55, CD59 and apoptotic cells were also analyzed.
  
  
  RESULTS
  The serum GPI-PLD activities, the protein and mRNA levels of GPI-PLD in HCC patients were decreased by 40%, 60% and 56%, respectively. The killing rate of CDC against the positive clone cells was significantly increased, but the proliferative capacity was obviously decreased. The apoptotic rate in positive clones was increased.
  
  
  CONCLUSIONS
  The expression of GPI-PLD decreases in HCC patients. The over-expression of GPI-PLD in HepG2 cells increases their sensitivity to CDC killing, impairs proliferative capacity and promotes apoptosis.
authors.affiliation: Department of Biochemistry, School of Biological Sciences and Technology, Central South University, 172 Tong Zi Po Road, Changsha, Hunan 410013, PR China. miket561@xysm.net
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Tang
authors.forename: Wang-Jiao
authors.initials: WJ
authors.lastname: He
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Chao-Chao
authors.initials: CC
authors.lastname: Tan
authors.forename: Qiong
authors.initials: Q
authors.lastname: Duan
authors.forename: Kai-Jia
authors.initials: KJ
authors.lastname: Wang
authors.forename: Xian-Yu
authors.initials: XY
authors.lastname: Yuan
authors.forename: Xu-Jin
authors.initials: XJ
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis; D018958:CD55 Antigens / Q000378:metabolism; D018957:CD59 Antigens / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D003165:Complement System Proteins / Q000276:immunology; D053938:DNA Fragmentation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000201:enzymology* / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008875:Middle Aged; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens; D017261:Glycosylphosphatidylinositols; D003165:Complement System Proteins; D000469:Alkaline Phosphatase; D010739:Phospholipase D; C065017:glycoprotein phospholipase D