title: Association between polymerization degree of apple peel polyphenols and inhibition of Helicobacter pylori urease.
abstract: Apple peel extracts and their fractions pooled according to their molecular size were prepared and evaluated for their inhibitory activity against Helicobacter pylori and Jack bean ureases. Urease Inhibitory effect of apple peel polyphenols (APPE) extracted from the Granny Smith variety was concentration-dependent and reversible. High molecular weight polyphenols (HMW) were more active against Helicobacter pylori and Jack bean ureases than low molecular weight polyphenols with IC50 values of 119 and 800 microg GAE/mL, respectively. The results suggest that monomeric compounds (mainly flavan-3-ols-and quercetin-O-glycosides) will not be implicated in the antiurease effect displayed by the apple peel polyphenolic extract. Thus, as a byproduct, apple peel is suitable for developing functional ingredients that could be useful for neutralizing an important Helicobacter pylori colonization factor.
authors.affiliation: Institute of Nutrition and Food Technology, University of Chile, Santiago.
authors.forename: Edgar
authors.initials: E
authors.lastname: Pastene
authors.forename: Miriam
authors.initials: M
authors.lastname: Troncoso
authors.forename: Guillermo
authors.initials: G
authors.lastname: Figueroa
authors.forename: Julio
authors.initials: J
authors.lastname: Alarcón
authors.forename: Hernán
authors.initials: H
authors.lastname: Speisky
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology*; D027845:Malus / Q000737:chemistry*; D008970:Molecular Weight; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology; D059808:Polyphenols; D014510:Urease / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; D059808:Polyphenols; D014510:Urease