Prenyl alcohol production by expression of exogenous isopentenyl diphosphate isomerase and farnesyl diphosphate synthase genes in Escherichia coli.
Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 microg/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 microg/l).
Ohto
Chikara
C
Bio Research Laboratory, Toyota Motor Corporation, Toyota.
Muramatsu
Masayoshi
M
Obata
Shusei
S
Sakuradani
Eiji
E
Shimizu
Sakayu
S
D019748:Carbon-Carbon Double Bond Isomerases / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005204:Farnesol / Q000378:metabolism; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes
D004224:Diterpenes; D029968:Escherichia coli Proteins; D045782:Hemiterpenes; D005204:Farnesol; C017338:geranylgeraniol; D051228:Geranyltranstransferase; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase