title: Temperature dependence of respiration in roots colonized by arbuscular mycorrhizal fungi.
abstract: * The arbuscular mycorrhizal (AM) symbiosis is ubiquitous, and the fungus represents a major pathway for carbon movement in the soil-plant system. Here, we investigated the impacts of AM colonization of Plantago lanceolata and temperature on the regulation of root respiration (R). * Warm-grown AM plants exhibited higher rates of R than did nonAM plants, irrespective of root mass. AM plants exhibited higher maximal rates of R (R(max)-R measured in the presence of an uncoupler and exogenous substrate) and greater proportional use of R(max) as a result of increased energy demand and/or substrate supply. The higher R values exhibited by AM plants were not associated with higher maximal rates of cytochrome c oxidase (COX) or protein abundance of either the COX or the alternative oxidase. * Arbuscular mycorrhizal colonization had no effect on the short-term temperature dependence (Q(10)) of R. Cold-acclimated nonAM plants exhibited higher rates of R than their warm-grown nonAM counterparts. By contrast, chilling had a negligible effect on R of AM-plants. Thus, AM plants exhibited less cold acclimation than their nonAM counterparts. * Overall, these results highlight the way in which AM colonization alters the underlying components of respiratory metabolism and the response of root R to sustained changes in growth temperature.
authors.affiliation: Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.
authors.forename: Owen K
authors.initials: OK
authors.lastname: Atkin
authors.affiliation: Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.
authors.forename: David
authors.initials: D
authors.lastname: Sherlock
authors.affiliation: Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.
authors.forename: Alastair H
authors.initials: AH
authors.lastname: Fitter
authors.affiliation: Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Jarvis
authors.affiliation: Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.
authors.forename: John K
authors.initials: JK
authors.lastname: Hughes
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden.
authors.forename: Catherine
authors.initials: C
authors.lastname: Campbell
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden.
authors.forename: Vaughan
authors.initials: V
authors.lastname: Hurry
authors.affiliation: Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.
authors.forename: Angela
authors.initials: A
authors.lastname: Hodge
mesh_terms: D000064:Acclimatization; D019069:Cell Respiration; D015169:Colony Count, Microbial; D003576:Electron Transport Complex IV / Q000378:metabolism; D024101:Mitochondrial Proteins; D038821:Mycorrhizae / Q000254:growth & development / Q000502:physiology*; D009929:Organ Size; D010088:Oxidoreductases / Q000378:metabolism; D010940:Plant Proteins; D010943:Plantago / Q000166:cytology / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology*; D012044:Regression Analysis; D013559:Symbiosis; D013696:Temperature; D000099188:Alternative Oxidase
chemical_list: D003576:Electron Transport Complex IV; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D000099188:Alternative Oxidase; C038862:cytochrome o oxidase