title: Multimeric hemicellulases facilitate biomass conversion.
abstract: Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover.
authors.affiliation: Department of Plant and Soil Sciences, and Kentucky Tobacco Research and Development Center, University of Kentucky, Cooper and University Drives, Lexington, KY 40546, USA.
authors.forename: Zhanmin
authors.initials: Z
authors.lastname: Fan
authors.forename: Kurt
authors.initials: K
authors.lastname: Wagschal
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Michael D
authors.initials: MD
authors.lastname: Montross
authors.forename: Charles C
authors.initials: CC
authors.lastname: Lee
authors.forename: Ling
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000595:Amino Acid Sequence; D018533:Biomass; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; C023305:hemicellulase; C019713:alpha-N-arabinofuranosidase; D043364:Endo-1,4-beta Xylanases