In vivo osteogenic differentiation of human adipose-derived stem cells in an injectable in situ-forming gel scaffold.
The sol-to-gel transition occurring at around body temperature makes the MPEG-PCL diblock copolymer an ideal candidate material for use as an injectable in situ-forming gel containing human adipose tissue-derived stem cells (hADSCs). The sol can be prepared at room temperature, and the gel forms at body temperature. Solutions of the copolymer containing hADSCs and osteogenic factors injected into rats formed gel scaffolds at the injection sites. The gels thus formed showed the interconnective pore structure required to support growth, proliferation, and differentiation of hADSCs. Bromodeoxyuridine-labeled hADSCs were confirmed to be present in gels formed in vivo. Bone formation was observed only in gel implants containing both hADSCs and osteogenic factors. Subcutaneous implantation of the in situ-forming gel scaffold demonstrated that hADSCs embedded in the gel stimulated much lower host tissue responses than did the gel alone, probably because of the unique immunomodulatory properties of hADSCs. In conclusion, our data on hADSCs embedded in an in situ gel scaffold suggest that this formulation may provide numerous benefits as a noninvasive alternative for tissue-engineered bone formation.
Ahn
Hyun Hee
HH
Fusion Biotechnology Research Center, Korea Research Institute of Chemical Technology, Daejeon, Korea.
Kim
Kyung Sook
KS
Lee
Jung Hwa
JH
Lee
Ju Young
JY
Kim
Byung Soo
BS
Lee
Il Woo
IW
Chun
Heung Jae
HJ
Kim
Jae Ho
JH
Lee
Hai Bang
HB
Kim
Moon Suk
MS
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D005260:Female; D005455:Fluorescent Antibody Technique; D005782:Gels; D006801:Humans; D007249:Inflammation; D007267:Injections; D007279:Injections, Subcutaneous; D010012:Osteogenesis; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D013696:Temperature; D054457:Tissue Scaffolds; D014783:Viscosity
D005782:Gels; D001973:Bromodeoxyuridine