Serotyping of foot-and-mouth disease virus by antigen capture-ELISA using monoclonal antibodies and chicken IgY.
Laboratory detection of specific foot-and-mouth disease virus (FMDV) is routinely carried out by ELISA and RT-PCR. Identification and serotyping of FMDV by ELISA requires polyclonal antibodies raised in rabbits and guinea pigs. The polyclonal antibodies have certain disadvantages such as batch to batch variation, inconsistent yields of antibodies and limited quantity of serum obtained from individual animals. This paper describes a method wherein monoclonal antibodies and chicken IgY were used in an antigen capture-ELISA for serotyping of thirty tongue epithelial samples and sixty tissue culture fluids. The results were compared with the routine antigen detection ELISA. The present study indicated that monoclonal antibodies and chicken IgY can substitute conventional polyclonal antibodies for routine serotyping of FMDV.
Veerasami
Maroudam
M
Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad, India.
Singanallur
Nagendrakumar B
NB
Thirumeni
Nagarajan
N
Rana
Samir K
SK
Shanmugham
Rajalakshmi
R
Ponsekaran
Santhakumar
S
Muthukrishnan
Madhanmohan
M
Villuppanoor
Srinivasan A
SA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D007136:Immunoglobulins / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; C016512:IgY; D007136:Immunoglobulins