title: Methotrexate-incorporated polymeric nanoparticles of methoxy poly(ethylene glycol)-grafted chitosan.
abstract: We prepared methotrexate (MTX)-encapsulated polymeric nanoparticles using methoxy poly(ethylene glycol) (MPEG)-grafted chitosan (ChitoPEG) copolymer. MTX-encapsulated polymeric nanoparticles of ChitoPEG copolymer has around 50-300nm in particle size and showed spherical shape when observed by transmission electron microscope (TEM). In (1)H nuclear magnetic resonance (NMR) study, the specific peaks of MTX and chitosan as a drug carrying inner-core disappeared at D(2)O and only the specific peak of MPEG was observed, while specific peaks of MPEG, MTX, and chitosan appeared in DCl/D(2)O mixtures. These results indicated that MTX was complexed with chitosan and then core-shell type nanoparticles had formed in aqueous environment, i.e., MTX/chitosan complexes composed of inner-core and MPEG composed of outer-shell of the nanoparticles. Loading efficiency of MTX in the polymeric nanoparticles was 94% (w/w) of ChitoPEG-1, 91.1% (w/w) of ChitoPEG-2, 90.1% (w/w) of ChitoPEG-3 and 65.2% (w/w) of ChitoPEG-4, expectively. The higher the drug feeding ratio, the higher the drug content and the lower the loading efficiency. The higher the MPEG graft ratio in the copolymer, the lower the drug content and loading efficiency. Drug contents evaluated by (1)H NMR were the same as found by UV spectrophotometer.
authors.affiliation: Department of Polymer Science and Engineering, Sunchon National University, Republic of Korea.
authors.forename: Dong-Hyuk
authors.initials: DH
authors.lastname: Seo
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.forename: Don-Gon
authors.initials: DG
authors.lastname: Kim
authors.forename: Min-Ja
authors.initials: MJ
authors.lastname: Jang
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Jang
authors.forename: Jae-Woon
authors.initials: JW
authors.lastname: Nah
mesh_terms: D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008727:Methotrexate / Q000008:administration & dosage* / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; C540518:methoxy poly(ethylene glycol)-grafted chitosan; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D048271:Chitosan; D008727:Methotrexate