title: Rapid identification of bacterial species with bacterial DNA microarray in cirrhotic patients with spontaneous bacterial peritonitis.
abstract: OBJECTIVE
  Early detection and identification of bacteria in ascitic fluid could result in more timely treatment of cirrhotic patients with spontaneous bacterial peritonitis (SBP) or subclinical SBP. The aim of this study was to evaluate the usefulness of a bacterial DNA microarray for the rapid diagnosis of SBP and rapid bacterial identification in cirrhotic patients with ascites.
  
  
  METHODS
  Thirty-seven cirrhotic patients with ascites (25 men and 12 women) participated. Ascitic fluid obtained from patients was tested by the bacterial DNA microarray method and by the conventional culture method.
  
  
  RESULTS
  SBP and bacterascites were diagnosed in 8 (16.7%) of 48 specimens by the conventional method. The bacterial DNA microarray proved the existence of bacteria in 6 (75%) of 8 samples with SBP or bacterascites using the conventional method as a gold standard. A corresponding rate of bacterial species identification between the two methods was found in 5 of 6 samples (83.3%). It took 1.47+/-0.96 and 5.14+/-2.6 days to receive the data by the microarray and conventional method, respectively (p<0.0001). After antibiotic therapy, the cumulative survival rate of recovered cases (n=8) was higher than that of unrecovered cases (n=5) (p=0.0008).
  
  
  CONCLUSIONS
  Although the detection rate of the bacterial DNA microarray was similar to the conventional culture method, the DNA microarray could identify pathogens about 4 times more rapidly than bacterial cultivation, thus rendering it useful for managing cirrhotic patients with ascites.
authors.affiliation: Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Sugihara
authors.forename: Masahiko
authors.initials: M
authors.lastname: Koda
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Maeda
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Matono
authors.forename: Takakazu
authors.initials: T
authors.lastname: Nagahara
authors.forename: Mari
authors.initials: M
authors.lastname: Mandai
authors.forename: Masaru
authors.initials: M
authors.lastname: Ueki
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Murawaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000382:microbiology; D001424:Bacterial Infections / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010538:Peritonitis / Q000175:diagnosis / Q000235:genetics* / Q000382:microbiology; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial