title: [Serological evidence of acute Toxoplasma gondii infection in pregnant women in Cascavel, Paraná].
abstract: In order to investigate the incidence of toxoplasmosis in Cascavel, Paraná, a city near the region where the largest reported epidemic outbreak in the world occurred, 334 serum samples from pregnant women were screened using a commercial IgG immunoenzymatic assay at the Municipal Laboratory in Cascavel and were confirmed at the Institute of Tropical Medicine in São Paulo, by means of IgG immunofluorescence, immunoenzymatic assaying and the in-house IgG avidity test. The IgG seropositivity from the commercial test was 54.2%, from immunofluorescence 54.8% and from the in-house IgG 53.9%, with good agreement between immunofluorescence and the commercial IgG test (kappa = 0.963781; co-positivity = 97.8%; co-negativity = 98,7%) and between immunofluorescence and the in-house IgG (kappa = 0.975857; co-positivity = 97.8%; co-negativity = 100%). The evidence of acute infection among the pregnant women was similar, as estimated both by IgG avidity (2.4%/year) and by statistical trend analysis (chi(2) test) according to age group (2%/year). This suggests that prenatal serological screening and epidemiological surveillance are essential for reducing the risk of toxoplasmosis in the region, although without evidence of an epidemic outbreak.
authors.affiliation: Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil. smioranza@usp.br
authors.forename: Sônia de Lucena
authors.initials: Sde L
authors.lastname: Mioranza
authors.forename: Luciana Regina
authors.initials: LR
authors.lastname: Meireles
authors.forename: Eduardo Luís
authors.initials: EL
authors.lastname: Mioranza
authors.forename: Heitor Franco de
authors.initials: HF
authors.lastname: Andrade Júnior
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000915:Antibody Affinity; D001938:Brazil / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis / Q000453:epidemiology*; D015995:Prevalence; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000453:epidemiology*; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M