title: [The change in Toll-like receptor 4 gene expression in skeleton of endotoxemia mice].
abstract: OBJECTIVE
  To explore the changes in gene expression of bone in endotoxemia in mice.
  
  
  METHODS
  Lipopolysaccharide (LPS) was injected intraperitoneally to reproduce an endotoxemia model in mice. Forty-eight mice were randomly divided into eight groups: normal group, 4, 6, 8, 12, 24, 48 and 72 hours after LPS injection groups, with 6 mice in each group. To evaluate endotoxemia whole blood was collected for leukocytic count, eye sockets blood was obtained for liver and renal functions tests. mRNA expression level of Toll-like receptor 4 (TLR4) in bone was determined by reverse transcription-polymerase chain reaction (RT-PCR) assay. The pathological changes of bone and tissue slides were prepared with hematoxylin and eosin (HE) staining for observing under microscope.
  
  
  RESULTS
  Compared with normal group, leukocyte count of 4-hour LPS-treated group was significantly decreased (P<0.01). However, after 4 hours, leukocyte count became higher gradually and remained at high level at 72 hours compared with that of normal group (P<0.05). Compared with normal group, alanine aminotransferase (ALT) level reached to a high level in LPS-treated groups at 4 hours and 6 hours (both P<0.05), and then decreased gradually showing a tendency to return to normal level after 8 hours (P>0.05). Blood urea nitrogen (BUN) was increased at 6 hours (P<0.05), reaching to the highest level at 8 hours (P<0.05) and tended to become normal level after 12 hours (P>0.05). Six hours after LPS treatment, the expression of TLR4 mRNA was enhanced (P<0.01) and reached the peak at 24 hours (P<0.01), and it remained at a high level at 72 hours (P<0.05). However, there were no significant pathological changes in bone structure after LPS treatment.
  
  
  CONCLUSIONS
  Expression level of TLR4 mRNA in bone is significantly increased in endotoxemia mice.
authors.affiliation: State Key Laboratory of Trauma, Burns, and Combined Injury, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Zi-yu
authors.initials: ZY
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Nan
authors.initials: N
authors.lastname: Su
authors.forename: Zi-xian
authors.initials: ZX
authors.lastname: Lei
authors.forename: Mao-mao
authors.initials: MM
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Fu-bing
authors.initials: FB
authors.lastname: Li
authors.forename: Yang-li
authors.initials: YL
authors.lastname: Xie
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4