Laser capture microdissection of kidney tissue.
Kidney tissue laser capture microdissection (LCM) is of great clinical relevance since genome wide studies on total kidney messenger RNA (mRNA) potentially miss important factors involved in the pathogenesis of the disease in glomeruli and tubules. This technique is readily applicable to study mRNA from isolated glomeruli and tubules of human kidney biopsy material. In this chapter we present a "cook-book" practical approach of utilizing LCM in combination with RNA isolation technique in downstream applications in nephrology.
Woroniecki
Robert P
RP
Department of Pediatrics, The Children's Hospital at Montefiore, Albert Einstein College of Medicine, New York, NY, USA.
Bottinger
Erwin P
EP
D006801:Humans; D007668:Kidney / Q000033:anatomy & histology / Q000502:physiology; D007674:Kidney Diseases / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification
D012333:RNA, Messenger