[Analysis of principal composition of ethyl acetate part in Huangqi Danggui decoction by HPLC-ESI-TOF-MS].
OBJECTIVE
To analyze and identify the principal composition of ethyl acetate part of Huangqi Danggui decoction by high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS).


METHODS
The analysis conditions are as follows: Zorbax SB C18 (4.6 mm x 250 mm, 5 microm) column; mobile phase (A) water mobile phase (B) acetonitrile, gradient elution; UV detection wavelength 254 nm; ESI source and data acquisition in positive and negative mode.


RESULTS
The accurate molecular weights of 20 compounds were measured and identified in ethyl acetate part of Huangqi Danggui decoction. Furthermore, the types of them are as below: flavonoids and flavonoid glycosides, saponins, oligosaccharides, amino acids and phthalides.


CONCLUSIONS
It is a rapid and accurate method that the compositions of compound prescription of traditional Chinese medicine can be identified in terms of the separation of high performance liquid chromatography, the accurate molecular weights measured by MS and other information, which can clarify the potential effective compounds of Huangqi Danggui decoction.
Xu
Li-Wei
LW
School of Pharmaceutical Science, Peking University, Beijing 100191, China.
Shang
Ming-Ying
MY
Li
Jun
J
Li
Xiao-Mei
XM
Meng
Li-Qiang
LQ
Cai
Shao-Qing
SQ
D000085:Acetates / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry; D001572:Benzofurans / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry; D012503:Saponins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000085:Acetates; D000596:Amino Acids; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D009844:Oligosaccharides; D012503:Saponins; C477279:danggui buxue decoction; C007650:ethyl acetate; C082022:phthalide