title: The effect of lidocaine on in vitro adhesion and migration of equine neutrophils.
abstract: The effect of lidocaine on in vitro migration and adhesion of equine neutrophils was evaluated. Neutrophils were isolated from equine whole blood using a Percoll-gradient centrifugation protocol. Purified neutrophils were incubated with lidocaine at concentrations from 0.1 to 1000 microg/ml for 30 min at 37 degrees C, after calcein loading. Neutrophil integrin-mediated adhesion in response to stimulation with 100 nM LTB(4), 100 nM PAF, or 100 ng/ml IL-8, or integrin-mediated migration in response to stimulation with 100 nM LTB(4), 150 nM PAF, or 100 ng/ml IL-8 was assessed. Statistical significance was set at P<0.05. Neutrophil adhesion was significantly increased in response to all three stimulants. IL-8-stimulated adhesion was significantly increased when neutrophils were incubated with 1mg/ml lidocaine, compared to lower lidocaine concentrations. LTB(4)-stimulated adhesion was significantly increased when neutrophils were incubated with 1mg/ml lidocaine compared to that at 5 microg/ml lidocaine. Migration was significantly increased in response to IL-8. IL-8 and LTB(4) stimulated migration was significantly increased when neutrophils were incubated with 1mg/ml lidocaine, compared to lower lidocaine concentrations. In conclusion, lidocaine did not inhibit neutrophil migration or adhesion in vitro at therapeutic concentrations, and increased migration and adhesion at higher concentrations.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA. vcook@cvm.msu.edu
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Cook
authors.forename: Laura E
authors.initials: LE
authors.lastname: Neuder
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Blikslager
authors.forename: Samuel L
authors.initials: SL
authors.lastname: Jones
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects* / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects* / Q000276:immunology; D006736:Horses / Q000097:blood / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D007975:Leukotriene B4 / Q000276:immunology; D008012:Lidocaine / Q000494:pharmacology*; D018375:Neutrophil Activation / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010972:Platelet Activating Factor / Q000276:immunology
chemical_list: D000779:Anesthetics, Local; D016209:Interleukin-8; D010972:Platelet Activating Factor; D007975:Leukotriene B4; D008012:Lidocaine