Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates.
Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains.
Logue
Carie-Anne
CA
Institute for Glycomics, Griffith University, Gold Coast Campus, Queensland 4222, Australia.
Peak
Ian R A
IR
Beacham
Ifor R
IR
D000483:Alleles; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D017384:Sequence Deletion; D013395:Sucrose / Q000378:metabolism*
D004269:DNA, Bacterial; D013395:Sucrose