Aqueous normal-phase retention of nucleotides on silica hydride columns.
The use of silica hydride-based stationary phases for the retention and analysis of nucleotides has been investigated. Both reversed-phase columns with a hydride surface underneath as well as those with an unmodified or a minimally modified hydride material were tested. With these systems, an aqueous normal-phase mode was used with high organic content mobile phases in combination with an additive to control pH for the retention of the hydrophilic nucleotides. Isocratic and gradient elution formats have been used to optimize separations for mixtures containing up to seven components. All conditions developed are suitable for methods that utilize mass spectrometry detection.
Pesek
Joseph J
JJ
Department of Chemistry, San Jose State University, San Jose, CA 95112, USA. pesek@sjsu.edu
Matyska
Maria T
MT
Hearn
Milton T W
MT
Boysen
Reinhard I
RI
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018130:Diamond / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D009711:Nucleotides / Q000032:analysis*; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D017640:Silicates / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D005227:Fatty Acids; D009711:Nucleotides; D017640:Silicates; C487012:silica hydride; D014867:Water; C016173:undecanoic acid; D018130:Diamond