title: Paraliobacillus quinghaiensis sp. nov., isolated from salt-lake sediment in China.
abstract: A Gram-positive, moderately halophilic, endospore-forming, catalase- and oxidase-positive, obligately aerobic bacterium, designated strain YIM-C158T, was isolated from sediment of a salt lake in the Qaidam Basin, north-west China. Cells were motile with peritrichous flagella and rod-shaped, with meso-diaminopimelic acid in the cell-wall peptidoglycan. Strain YIM-C158T grew in the presence of 1-20 % (w/v) NaCl and pH 6.0-10.0, with optimum growth at 5 % (w/v) NaCl and pH 8.0. The strain grew at 4-50 degrees C, with optimum growth at 37 degrees C. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(14 : 0), C(16 : 0), anteiso-C(17 : 0), iso-C(16 : 0), C(16 : 1)omega7c alcohol and C(16 : 1)omega11c. Strain YIM-C158T contained menaquinone MK-7 as the sole respiratory quinone and phosphatidylmethylethanolamine and phosphatidylcholine as the polar lipids. The genomic DNA G+C content was 39.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-C158T was most closely related to Paraliobacillus ryukyuensis DSM 15140T (96.8 % similarity), and the two strains formed a distinct branch in the phylogenetic tree. The level of DNA-DNA relatedness between the two strains was 15.6 %. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic differences and DNA-DNA hybridization data supported the view that strain YIM-C158T represents a novel species of the genus Paraliobacillus, for which the name Paraliobacillus quinghaiensis sp. nov. is proposed. The type strain is YIM-C158T (=DSM 17857T=CGMCC 1.6333T).
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Cui
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Meng-Liang
authors.initials: ML
authors.lastname: Wen
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride