Effects of gonadotropins on in vitro maturation and of electrical stimulation on parthenogenesis of canine oocytes.
The objective of this study was to determine the effects of gonadotropins on in vitro maturation (IVM) and electrical stimulation on the parthenogenesis of canine oocytes. In experiment I, cumulus oocyte complexes were collected from ovaries at a random phase of the oestrus cycle and cultured on maturation medium treated with hCG or eCG for 48 or 72 h. There were no significant differences in the effects on the metaphase II (MII) rate between the hCG and eCG treatment groups over 48 h (5.4% vs 5.5%). The MII rate in the co-treatment group of hCG and eCG for 48 h was higher than in each hormone treated group (15.5%, p < 0.05). In experiment 2, the parthenogenetic effect on oocyte development, at various electrical field strengths (1.0, 1.5, 2.0 kV/cm DC) for 60 or 80 mus with a single DC pulse after IVM on the co-treatment of hCG and eCG, was examined. The rate of pronuclear formation (37.1%) in electrical activation at 1.5 kV/60 mus without cytochalasin B (CB) was higher than that of oocytes activated in the other groups (p < 0.05). However, we did not observe the cleavage stages. Also, CB did not influence parthenogenesis of canine oocytes. The results showed that the pronucleus formation rate, indicative of the parthenogenesis start point, could be increased by electrical stimulation. Therefore, these results can provide important data for the parthenogenesis of canine oocytes and suggest the probability of parthenogenesis in canines.
Kim
B S
BS
School of Life Science and Biotechnology, Kyungpook National University, Buk-ku, South Korea.
Lee
S R
SR
Hyun
B H
BH
Shin
M J
MJ
Yoo
D H
DH
Lee
S
S
Park
Y S
YS
Ha
J H
JH
Ryoo
Z Y
ZY
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D004285:Dogs; D004558:Electric Stimulation / Q000379:methods; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008677:Metaphase / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D010312:Parthenogenesis / Q000502:physiology*
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D003571:Cytochalasin B