Pranlukast attenuates ischemia-like injury in endothelial cells via inhibiting reactive oxygen species production and nuclear factor-kappaB activation.
The anti-inflammatory effects of pranlukast, an antagonist of cysteinyl leukotriene receptor 1, may be rendered not only by antileukotriene activity but also by other pharmacological activities. Previous studies indicate that pranlukast reduces ischemic tissue injury partially through decreasing vascular permeability, but its effect on ischemic injury in endothelial cells is not known. Thus, in this study, we investigated the effect of pranlukast on ischemia-like injury induced by oxygen-glucose deprivation (OGD) in EA.hy926 cells, a human endothelial cell line, and the possible mechanisms. We found that cell viability was reduced, lactate dehydrogenase release was increased 4-8 hours after OGD, and necrosis was induced 8 hours after OGD. Production of reactive oxygen species (ROS) increased by 211%, 176%, and 128%, respectively, 0.5, 1, and 2 hours after OGD. Nuclear factor-kappaB (NF-kappaB) was translocated to the nuclei 4-8 hours after OGD. Pranlukast ameliorated the reduced viability, the increased lactate dehydrogenase release, and necrosis after OGD. It also reduced ROS production and inhibited NF-kappaB nuclear translocation after OGD. The ROS scavenger, edaravone, inhibited OGD-induced nuclear translocation of NF-kappaB as well. Edaravone and pyrrolidine dithiocarbamate (a specific NF-kappaB inhibitor) protected endothelial cells from the OGD-induced injury. However, zileuton, a 5-lipoxygenase inhibitor, did not affect the cell injury, ROS production, and NF-kappaB nuclear translocation after OGD. The exogenous leukotriene D4 did not induce cell injury, ROS production, and NF-kappaB translocation. Thus, we conclude that pranlukast protects endothelial cells from ischemia-like injury via decreasing ROS production and inhibiting NF-kappaB activation, which is leukotriene independent.
Fang
San-Hua
SH
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou, China.
Yuan
Yu-Mei
YM
Peng
Fang
F
Li
Cheng-Tan
CT
Zhang
Li-Hui
LH
Lu
Yun-Bi
YB
Zhang
Wei-Ping
WP
Wei
Er-Qing
EQ
D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002867:Chromones; D042783:Endothelial Cells / Q000378:metabolism; D005947:Glucose / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007511:Ischemia / Q000235:genetics / Q000378:metabolism; D020024:Leukotriene Antagonists / Q000378:metabolism / Q000494:pharmacology; D017998:Leukotriene D4 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015289:Leukotrienes / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D009336:Necrosis / Q000235:genetics / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism* / Q000494:pharmacology
D002867:Chromones; D020024:Leukotriene Antagonists; D015289:Leukotrienes; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D017998:Leukotriene D4; D001094:Arachidonate 5-Lipoxygenase; D005947:Glucose; D010100:Oxygen; C047681:pranlukast