title: Influence of tooth age and root section on root dentine dye penetration.
abstract: OBJECTIVE
  To investigate dye permeability of root dentine according to patients' age, root section and dye penetration time.
  
  
  METHODS
  A total of 96 extracted human single-rooted teeth, assigned to four age groups (<30, 30-45, 45-60 and >60 years) were separated at the cemento-enamel junction and root canals were enlarged. The root surfaces were coated with cyanocrylate to prevent external dye penetration and centrifuged in distilled water to eliminate air. For dye penetration the root canals were filled with methylene blue 5%. After 1, 30 and 60 days eight roots per age group were cross-sectioned in 1 mm slices. Dye penetrated areas and the complete dentine areas were digitized and measured. Differences between groups were judged with anova and LSD, P < 0.05 or P < 0.01.
  
  
  RESULTS
  The root section, the patients' age and the penetration time influenced significantly the penetrated areas (P < 0.05). After 1 and 30 days significant differences could be found only in the apical root sections between all age groups (P < 0.05). Dye penetration areas systematically decreased with increasing age and also from coronal to apical (P < 0.01).
  
  
  CONCLUSIONS
  Age influenced dye penetration significantly. Dye penetration also depended on the location (coronal, middle and apical) within the root canal. These findings indicate that there may be a correlation between the tooth age and permeability of root dentine, which may influence the distribution and effectiveness of drugs used for root canal disinfection.
authors.affiliation: Dental Clinic 1, Operative Dentistry and Periodontology, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Thaler
authors.forename: J
authors.initials: J
authors.lastname: Ebert
authors.forename: A
authors.initials: A
authors.lastname: Petschelt
authors.forename: M
authors.initials: M
authors.lastname: Pelka
mesh_terms: D000328:Adult; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D002114:Calcinosis / Q000378:metabolism / Q000473:pathology; D004396:Coloring Agents; D003786:Dental Pulp Cavity / Q000378:metabolism / Q000473:pathology*; D003804:Dentin / Q000378:metabolism / Q000473:pathology*; D003806:Dentin Permeability / Q000502:physiology; D003809:Dentin, Secondary / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008751:Methylene Blue; D008875:Middle Aged; D023861:Photography, Dental; D018915:Root Canal Preparation / Q000379:methods; D013997:Time Factors; D019227:Tooth Apex / Q000378:metabolism / Q000473:pathology; D014092:Tooth Root / Q000378:metabolism / Q000473:pathology*
chemical_list: D004396:Coloring Agents; D008751:Methylene Blue