Oxidase-like activity of polymer-coated cerium oxide nanoparticles.
Inorganic enzyme? Ceria nanoparticles exhibit unique oxidase-like activity at acidic pH values. These redox catalysts can be used in immunoassays (ELISA) when modified with targeting ligands (see picture; light blue and yellow structures are nanoparticles with attached ligands). This modification allows both for binding and for detection by the catalytic oxidation of sensitive colorimetric dyes (e.g. TMB).
Asati
Atul
A
Nanoscience Technology Center, Department of Chemistry and Burnett College of Biomedical Sciences, University of Central Florida, 12424 Research Parkway, Suite 400, Orlando, FL 32826, USA.
Santra
Santimukul
S
Kaittanis
Charalambos
C
Nath
Sudip
S
Perez
J Manuel
JM
D002384:Catalysis; D045744:Cell Line, Tumor; D002563:Cerium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*
D005456:Fluorescent Dyes; D011108:Polymers; D002563:Cerium; C030583:ceric oxide; D010088:Oxidoreductases