[Construction of an alr3504 defected mutant and its phenotype analysis in anabaena PCC7120].
C-di-GMP [Bis-(3',5')-cyclic dimeric guanosine monophosphate] is a novel global second messenger in bacteria. The diguanylate cyclase and phosphodiesterase involve in c-di-GMP synthesis and degradation, respectively. alr3504 in Anabaena PCC7120 possibly encodes diguanylate cyclase with a coserved GGDEF domain. [Objective] For functional characterization, [Methods] the deletion mutant of alr3504 was constructed by using marker exchange method. [Results]No significant differences were found in the cell morphology, growth rate or heterocyst development between the deletion mutant and the wild type strain. But the mutant was more sensitive to Na'-salt under salt-stress. [Conclusion] The results show that alr3504 did not affect heterocyst development directly, but involved in other signaling pathway, which lay a foundation for exploring the abundant and complex signal transduction of cyanobacteria.
Dai
Yan
Y
College of Life Science and Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. dy1567@webmail.hzau.edu.cn
Wang
Li
L
Chen
Wenli
W
D017033:Anabaena / Q000201:enzymology* / Q000378:metabolism; D006152:Cyclic GMP / Q000737:chemistry*; D029968:Escherichia coli Proteins; D010641:Phenotype; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D019761:Phosphorus-Oxygen Lyases / Q000378:metabolism*; D015290:Second Messenger Systems / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
D029968:Escherichia coli Proteins; D010727:Phosphoric Diester Hydrolases; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP