[Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice].
OBJECTIVE
To investigate the protective effects of the n-butanol extract of Potentilla anserina L. (NP) on pituitrin-induced acute myocardial ischemic injury in mice.


METHODS
Ninety healthy female mice were randomly divided into normal control group, untreated group, Salvia miltiorrhiza group and low-, medium- and high-dose NP groups. Except for the normal control group, the mice were intraperitoneally injected with pituitrin (20 U/kg) to induce acute myocardial ischemic injury. Thirty minutes after induction, electrocardiogram was monitored, and height of the J spot was measured also. Activities of lactate dehydrogenase (LDH), creatine kinase (CK) and superoxide dismutase (SOD) and content of malondialdehyde (MDA) in serum of the mice were detected. The degree of myocardial ischemic injury in mice was observed by Nagar-Olsen staining.


RESULTS
The moving up of J spots in the treated groups was significantly inhibited when comparing with the untreated group (P<0.01). Compared with untreated group, high- and medium-dose NP and Salvia miltiorrhiza could significantly decrease the activities of LDH, CK (P<0.01, P<0.05), increase the SOD activity (P<0.01) and decrease the content of MDA (P<0.05). However, no significant difference was observed between low-dose NP group and untreated group (P>0.05). Nagar-Olsen staining showed that high- and medium-dose NP and Salvia miltiorrhiza could significantly diminish the areas of cardiac muscles injured by ischemia, but low-dose NP had no effect on that.


CONCLUSIONS
NP has a remarkable protective effect on acute myocardial ischemic injury in mice.
Li
Jian-yu
JY
Department of Preventive Medicine, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China. lijianyu118@163.com
Li
Yi
Y
Gong
Hai-ying
HY
Zhao
Xiao-bo
XB
Li
Ling-zhi
LZ
D020001:1-Butanol; D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008815:Mice, Inbred Strains; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology*; D009206:Myocardium / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D031987:Potentilla / Q000737:chemistry*
D010936:Plant Extracts; D008315:Malondialdehyde; D020001:1-Butanol; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase