title: Differential binding profiles of StpA in wild-type and h-ns mutant cells: a comparative analysis of cooperative partners by chromatin immunoprecipitation-microarray analysis.
abstract: We performed chromatin immunoprecipitation-microarray analysis to investigate differences in function between StpA and H-NS in Escherichia coli cells. StpA binding regions essentially overlap those of H-NS in wild-type cells, while they are reduced to one-third in the hns mutant. The H-NS binding profile was unaffected by stpA inactivation.
authors.affiliation: Graduate School of Information Science, Nara Institute of Science and Technology, Japan.
authors.forename: Ebru
authors.initials: E
authors.lastname: Uyar
authors.forename: Ken
authors.initials: K
authors.lastname: Kurokawa
authors.forename: Mika
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Shu
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Naotake
authors.initials: N
authors.lastname: Ogasawara
authors.forename: Taku
authors.initials: T
authors.lastname: Oshima
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C031908:H-NS protein, bacteria; D018832:Molecular Chaperones; C090104:StpA protein, E coli