[Investigation on inhibition of HIV III B virus with extractions of Juglans regia].
OBJECTIVE
To study the effect of anti-HIV III B virus with extractions from Juglans regia, so as to searching for the new and efficacious leading compound of AIDS therapy.


METHODS
Phytochemical and chromatographical techniques were used to isolate compounds from J. regia; MT4 cells and HIV III B virus were used to study the effect of anti-HIV activity in vitro. BIACORE 3000 molecule coupled equipment were used for the target research also.


RESULTS
Two extractions (B&E) were isolated from J. regia which possess the effect of anti-HIV activity. Targets study found that extraction B could affected on HIV-1 gp-41 fusing protein and extraction E could affected on HIV-1 integrase respectively.


CONCLUSIONS
J. regia is a traditional Chinese medicine with active anti-HIV activity and worth to be studied further.
Liu
Zhao-Mei
ZM
Department of Virology & Pharmacology, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China.
Wen
Rui-Xing
RX
Ma
Hong-Tao
HT
Yang
Yi-Shu
YS
Wang
Xiao-Li
XL
Lv
Xiu-Hua
XH
Li
Ze-Lin
ZL
D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D031324:Juglans / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts