Ex vivo expansion of hematopoietic stem cell by fusion protein TAT-Zfx.
The relative inability of hemopoietic stem cells (HSCs) to reproduce themselves (self-renew) ex vivo imposes substantial limitations on the current use of HSC transplantation. Recently, the transcription factor Zfx has been demonstrated that played an important in controlling the self-renewal of hematopoietic stem cells. Here, we reported that Zfx could enable high-level expansion of HSCs in vitro, by combination of protein transduction domain, TAT. Furthermore, we also demonstrated that expanded HSCs population retains their normal in vivo potential of pluripotency. It is thus that TAT-Zfx has the potential to expand HSCs significantly in vitro, and will have enormous clinical potentials.
Xu
Chong
C
Laboratory Diagnostic Center, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
Zhang
Yanbing
Y
Jiang
Hua
H
D000595:Amino Acid Sequence; D000818:Animals; D049452:Green Fluorescent Proteins / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000502:physiology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D017434:Protein Structure, Tertiary / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology*; D012038:Regeneration; D014161:Transduction, Genetic
D051741:Kruppel-Like Transcription Factors; D011993:Recombinant Fusion Proteins; C062076:zinc finger protein, X-linked; D049452:Green Fluorescent Proteins