title: Paenibacillus contaminans sp. nov., isolated from a contaminated laboratory plate.
abstract: A bacterial strain, designated CKOBP-6T, isolated from a contaminated laboratory plate, was characterized by using a polyphasic taxonomic approach. Chemotaxonomic and morphological properties of the strain were consistent with its classification in the genus Paenibacillus. Cells were Gram-variable, aerobic, sporulating, motile rods. 16S rRNA gene sequence analysis demonstrated that this novel isolate was unique, showing 94.6 % sequence similarity to Paenibacillus chitinolyticus NBRC 15660T and lower levels of similarity to Paenibacillus gansuensis KCTC 3950T (93.8 %) and Paenibacillus terrigena A35T (93.8 %). The novel isolate could be distinguished from the type strains of all of these species based on a range of phenotypic data. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one unknown phosphoglycolipid. The predominant isoprenologue was an unsaturated menaquinone with seven isoprene units (MK-7). The major fatty acids of strain CKOBP-6T were C(16 : 0) (12.0 %), anteiso-C(15 : 0) (52.0 %) and iso-C(16 : 0) (16.4 %). The G+C content of the genomic DNA was 51.2 mol%. It is evident from the genotypic and phenotypic data that strain CKOBP-6T should be classified as a representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus contaminans sp. nov. is proposed. The type strain is CKOBP-6T (=BCRC 17728T=LMG 24216T).
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.forename: Jo-Hsin
authors.initials: JH
authors.lastname: Lee
authors.forename: Mei-Chun
authors.initials: MC
authors.lastname: Lin
authors.forename: Poh-Shing
authors.initials: PS
authors.lastname: Chang
authors.forename: A B
authors.initials: AB
authors.lastname: Arun
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004866:Equipment Contamination; D020459:Genes, rRNA; D005838:Genotype; D007753:Laboratories; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S