title: Changes in cytokine concentrations in graft renal vein during reperfusion in patients with and without delayed graft function.
abstract: BACKGROUND
  The impairment of organ function derived from ischemia-reperfusion injury is still an important problem in solid organ transplantation. Cell alterations induced by ischemia prime the tissue for the subsequent damage that occurs during the reperfusion phase. Despite recent advances in immunosuppressive therapy, delayed graft function (DGF) remains an important problem after kidney transplantation. Different studies have related various clinical factors to DGF, such as donor age, recipient age, cold ischemia time, initial immunosuppressive regimens. The aim of present study was to examine the changes in cytokine concentrations in graft renal vein during the reperfusion in relation to the development of delayed graft function.
  
  
  METHODS
  The study included 17 recipients of cadaveric renal grafts (10 males, 7 females, mean age 49 +/- 7 years, cold ischemia time 25 +/- 3 h)--8 with DGF and 9 without DGF. Levels of IL-lbeta, IL-2, IL-4, IL-6, IL-8, IL-10, IFN-gamma, TNF-beta and TNF-alpha in renal graft vein plasma during 5 first min. of reperfusion were quantified by flow-cytometry.
  
  
  RESULTS
  The increased concentrations ofIL-6, TNF-alpha and IL-1beta were observed during reperfusion. However there were no statistically significant differences between patients with and without DGF.
authors.affiliation: Klinika Nefrologii, Transplantologii i Chorób Wewnetrznych Pomorskiej Akademii Medycznej w Szczecinie al. Powstańców Wlkp. 72, 70-111 Szczecin.
authors.forename: Leszek
authors.initials: L
authors.lastname: Domański
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pawlik
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Jacek
authors.initials: J
authors.lastname: Rozański
authors.forename: Marek
authors.initials: M
authors.lastname: Myślak
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Sulikowski
authors.forename: Maciej
authors.initials: M
authors.lastname: Romanowski
authors.forename: Marek
authors.initials: M
authors.lastname: Ostrowski
authors.forename: Magda
authors.initials: M
authors.lastname: Wiśniewska
authors.forename: Maciej
authors.initials: M
authors.lastname: Domański
authors.forename: Joanna
authors.initials: J
authors.lastname: Kabat-Koperska
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ciechanowski
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D051799:Delayed Graft Function / Q000209:etiology / Q000378:metabolism*; D005260:Female; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D012082:Renal Veins / Q000378:metabolism* / Q000637:transplantation*; D015424:Reperfusion
chemical_list: D016207:Cytokines