title: Prion disease diagnosis by proteomic profiling.
abstract: Definitive prion disease diagnosis is currently limited to postmortem assay for the presence of the disease-associated proteinase K-resistant prion protein. Using cerebrospinal fluid (CSF) from prion-infected hamsters, matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS), and support vector machines (SVM), we have identified peptide profiles characteristic of disease state. Using 10-fold leave-one-out cross-validation, we report a predictive accuracy of 72% with a true positive rate of 73% and a false positive rate of 27% demonstrating the suitability of using proteomic profiling and CSF for the development of multiple marker diagnostics of prion disease.
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin-Madison, Wisconsin 53705-222, USA.
authors.forename: Allen
authors.initials: A
authors.lastname: Herbst
authors.forename: Sean
authors.initials: S
authors.lastname: McIlwain
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Schmidt
authors.forename: Judd M
authors.initials: JM
authors.lastname: Aiken
authors.forename: C David
authors.initials: CD
authors.lastname: Page
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid; D006224:Cricetinae; D006801:Humans; D017096:Prion Diseases / Q000175:diagnosis*; D011328:Prions / Q000134:cerebrospinal fluid*; D040081:Protein Array Analysis / Q000379:methods / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D011328:Prions