General methods for flash chromatography using disposable columns.
As technology has evolved available guidelines for normal-phase flash chromatography have become less relevant. Years of experience performing chromatography with disposable columns have been condensed into simple guidelines useful for translating TLC results into either isocratic- or gradient-flash chromatography. The described studies should provide researchers with a means of selecting adequate columns and guidelines to reduce the waste of solvents, silica, time, and money.
Stevens
William C
WC
Exelixis, Inc., San Diego, CA, USA. bstevens02@earthlink.net
Hill
Daniel C
DC
D002845:Chromatography / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D004209:Disposable Equipment; D009930:Organic Chemicals / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
D009930:Organic Chemicals; D012997:Solvents