Ultra-high concentration of amylose for chiral separations in capillary electrophoresis.
In the present study, a capillary electrophoresis method using high concentration of amylose solutions as separation medium has been developed with the aid of dimethyl sulfoxide (DMSO) as co-solvent. The best buffer conditions for primaquine, trihexyphenidyl (THP), sulconazole and cetirizine enantiomers were optimized as 20mM sodium phosphate buffer with DMSO/water (40/60, v/v) as solvent at a pH of 3.0, containing 10% (w/v) amylose. Partial-filling and semi-permanent coating techniques were used considering the influences of DMSO on UV detection. High chiral resolution for THP enantiomers was obtained showing good chiral separation capacity of this method. The method showed good linearity (R(2) > 0.998) over the concentration range of 0.50 and 2.00 mg L(-1) for all the enantiomers. The detection limits for the tested enantiomers were in the range from 0.05 to 0.12 mg L(-1). The linear calibration models were proven to be adequate for the experimental data by lack-of-fit test. The intra-assay precision, inter-day precision and accuracy were all evaluated to be acceptable. Separation and determination of THP enantiomers in rabbit blood were also carried out.
Wei
Wei-Li
WL
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Guo
Bao-Yuan
BY
Lin
Jin-Ming
JM
D000688:Amylose / Q000737:chemistry*; D000704:Analysis of Variance; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
D014867:Water; D000688:Amylose; D004121:Dimethyl Sulfoxide