beta-glucosidase catalyzed syntheses of pyridoxine glycosides.
Enzymatic syntheses of pyridoxine glycosides were carried out in di-isopropyl ether organic medium using beta-glucosidase isolated from sweet almond. Optimum conditions determined for the reaction with D-glucose were 40% (w/w D-glucose) beta-glucosidase at 0.18 mM (1.8 ml) of pH 5 acetate buffer over a 72 h incubation period. Of 11 carbohydrates employed, beta-glucosidase gave 7-O-(alpha-D-glucopyranosyl)pyridoxine 5a, 7-O-(beta-D-glucopyranosyl)pyridoxine 5b, 6-O-(alpha-D-glucopyranosyl)pyridoxine 5c, 7-O-(alpha-D-galactopyranosyl)pyridoxine 6a, 7-O-(beta-D-galactopyranosyl)pyridoxine 6b, 6-O-(alpha-D-galactopyranosyl)pyridoxine 6c, 7-O-(alpha-D-mannopyranosyl)pyridoxine 7a, 7-O-(beta-D-mannopyranosyl)pyridoxine 7b, and 6-O-(alpha-D-mannopyranosyl)pyridoxine 7c in yields ranging from 23 to 40%.
Charles
Rajachristu Einstein
RE
Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India.
Divakar
Soundar
S
D002241:Carbohydrates; D006027:Glycosides / Q000032:analysis / Q000138:chemical synthesis*; D027861:Prunus / Q000201:enzymology; D011736:Pyridoxine / Q000032:analysis / Q000138:chemical synthesis*; D012997:Solvents; D001617:beta-Glucosidase / Q000378:metabolism*
D002241:Carbohydrates; D006027:Glycosides; D012997:Solvents; D001617:beta-Glucosidase; D011736:Pyridoxine