Polymerized placenta hemoglobin improves cardiac functional recovery and reduces infarction size of isolated rat heart.
OBJECTIVE
To investigate the cardioprotective effect of polymerized human placenta hemoglobin (PolyPHb) to rat heart subjected to 8-hour hypothermic storage and 2-hour normothermic reperfusion.


RESULTS
Isolated rat hearts were perfused with Langendorff model; after 30 minutes of baseline, the hearts were arrested and stored by St. Thomas' solution (STS) without (STS group) or with 0.5 gHb/dL PolyPHb (PolyPHb group) at 4 degrees C for 8 hours, then reperfused for 2 hours. Compared with STS group, PolyPHb in STS greatly improved the recovery of left ventricular developed pressure (LVDP), maximum LVDP increase and decrease rate (+/-dp/dt), coronary flow rate (CF). Also, both the cardiac enzyme release, including creatine kinase (CK) and lactate dehydrogenase (LDH), and myocardial infarction size were significantly reduced in PolyPHb group.


CONCLUSIONS
Our study demonstrated that the PolyPHb was beneficial to improving cardiac functional recovery and reducing myocardial infarction of 8-hour hypothermic stored rat heart.
Li
Tao
T
Laboratory of Anesthesiology and Critical Care Medicine, West China School of Pharmacy, Sichuan University, Chengdu, China.
Liu
Jin
J
Yang
Qian
Q
Wu
Wei
W
Zhang
Pu
P
Yang
Jing
J
Li
Jing
J
Zhang
Wensheng
W
Yang
Chengmin
C
D000818:Animals; D005260:Female; D006321:Heart / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007035:Hypothermia; D066298:In Vitro Techniques; D008297:Male; D009203:Myocardial Infarction / Q000201:enzymology / Q000473:pathology* / Q000503:physiopathology; D009206:Myocardium / Q000201:enzymology; D010920:Placenta; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D015424:Reperfusion; D013997:Time Factors; D016277:Ventricular Function, Left / Q000187:drug effects
D006454:Hemoglobins