Involvement of phospholipase D in the low temperature acclimation-induced thermotolerance in grape berry.
Both phospholipase D (PLD, EC 3.1.4.4) and salicylic acid (SA) play important roles in response to external stimulation and activating defense system in plants. However, roles of the two signals in plants during the development of thermotolerance induced by low temperature acclimation remain unclear. In the experiment presented in the paper, grape berries (Vitis vinifera L. cv. Chardonnay) were pretreated at 8 degrees C for 3h and then transferred to 45 degrees C for heat stress. Compared with the control without low temperature pretreatment, membrane permeability and malondialdehyde (MDA) contents were reduced and the expression of HSP73 increased in the low temperature-pretreated berries under heat stress. During low temperature acclimation, PLD, SA and HSP73 could be activated. Meanwhile, the expression of HSP73 and the accumulation of free SA induced by low temperature can be inhibited by PLD activity inhibitor. All these results suggest that the activation of PLD is an early response to low temperature, and it is involved in the accumulation of free SA and the development of thermotolerance induced by low temperature acclimation.
Wan
Si-Bao
SB
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Tian
Li
L
Tian
Rong-Rong
RR
Pan
Qiu-Hong
QH
Zhan
Ji-Cheng
JC
Wen
Peng-Fei
PF
Chen
Jian-Ye
JY
Zhang
Ping
P
Wang
Wei
W
Huang
Wei-Dong
WD
D020001:1-Butanol / Q000378:metabolism; D000064:Acclimatization; D002462:Cell Membrane / Q000502:physiology; D003080:Cold Temperature; D005638:Fruit / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D008315:Malondialdehyde / Q000378:metabolism; D010539:Permeability; D010739:Phospholipase D / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*; D027843:Vitis / Q000201:enzymology* / Q000502:physiology
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D008315:Malondialdehyde; D020001:1-Butanol; D010739:Phospholipase D; D020156:Salicylic Acid