title: Fucoidan inhibits parainfluenza virus type 2 infection to LLCMK2 cells.
abstract: The effects of fucoidan and L-fucose, a fundamental major component of fucoidan, on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK(2) cells were investigated. Fucoidan inhibited cell fusion and hemadsorption, but L-fucose only partly inhibited both. Virus RNA was not detected in the hPIV-2 infected cells cultured with fucoidan. However, L-fucose did not inhibit virus RNA synthesis. Indirect immunofluorescence study showed that virus protein synthesis was inhibited by fucoidan, but not by L-fucose. Furthermore, using a recombinant, green fluorescence protein-expressing hPIV-2, it was found that virus entry was inhibited by fucoidan, but not by L-fucose. These results suggested that fucoidan inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented infection, indicating that the sulfated polysaccharide form was important for the inhibition by fucoidan.
authors.affiliation: Department of Microbiology, Suzuka University of Medical Science, Suzuka, Mie, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Taoda
authors.forename: Eri
authors.initials: E
authors.lastname: Shinji
authors.forename: Kumiko
authors.initials: K
authors.lastname: Nishii
authors.forename: Saori
authors.initials: S
authors.lastname: Nishioka
authors.forename: Yukari
authors.initials: Y
authors.lastname: Yonezawa
authors.forename: Jun
authors.initials: J
authors.lastname: Uematsu
authors.forename: Emi
authors.initials: E
authors.lastname: Hattori
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kawano
authors.forename: Masato
authors.initials: M
authors.lastname: Tsurudome
authors.forename: Myles
authors.initials: M
authors.lastname: O'Brien
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komada
mesh_terms: D002460:Cell Line; D002648:Child; D005643:Fucose / Q000378:metabolism; D006801:Humans; D010223:Parainfluenza Virus 2, Human / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D019351:Rubulavirus Infections / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014764:Viral Proteins; D005643:Fucose; C007789:fucoidan