[Effects of different enteral nutrition formulae combined with recombinant human growth hormone on inflammatory response in rats with scald injury].
OBJECTIVE
To investigate the effects of enteral immunonutrition or enteral nutrition combined with recombinant human growth hormone (rhGH) on inflammatory response in rats with severe scald.


METHODS
One hundred and twenty-eight SD rats were inflicted with 30% TBSA full-thickness scald, then they were divided into enteral nutrition (EN), enteral immunonutrition (EIN), EN combined with rhGH (EN + rhGH) and EIN combined with rhGH (EIN + rhGH) groups according to random number table. The serum levels of endotoxin, IL-6 and TNF-alpha as well as the expression of CD14 and TNF-alpha mRNA in liver were determined on the 1, 4, 7, 10 post scald day (PSD). Eight normal SD rats served as control, and above indices were also examined in this group (C group).


RESULTS
The levels of endotoxin, IL-6 and TNF-alpha as well as the expression of CD14mRNA, TNF-alphamRNA in EN, EIN, EN + rhGH, EIN + rhGH groups were remarkably higher than those of C group at each time point. The levels of endotoxin, IL-6 and TNF-alpha as well as the expression of CD14 mRNA, TNF-alpha mRNA in EIN and EN + rhGH groups were lowered significantly on 4, 7 and 10 PSD compared with those of EN group (P < 0.05 or P < 0.01). The levels of endotoxin (0.37 +/- 0.07 EU/mL), IL-6 (289 +/- 49 ng/L) and TNF-alpha (1.87 +/- 0.32 microg/L) as well as the expression of CD14 mRNA (0.39 +/- 0.05), TNF-alpha mRNA (0.47 +/- 0.03) in EIN + rhGH group were also obviously lower than those [(0.48 +/- 0.08) EU/mL, (364 +/- 53) ng/L, (2.50 +/- 0.48) microg/L, 0.67 +/- 0.06, 0.66 +/- 0.05, P < 0.05 or P < 0.01, respectively] in EIN group on 10 PSD (P < 0.05 or P < 0.01).


CONCLUSIONS
Enteral immunonutrition combined with rhGH can ameliorate inflammatory response, in which rhGH has a collaborative effect.
Guo
Guang-hua
GH
Department of Burns, the First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
Cai
Chen
C
Zhang
Hong-yan
HY
Fu
Zhong-hua
ZH
Xu
Cheng
C
D000818:Animals; D002056:Burns / Q000097:blood / Q000628:therapy*; D004195:Disease Models, Animal; D004731:Endotoxins / Q000097:blood; D004750:Enteral Nutrition / Q000379:methods*; D019382:Human Growth Hormone / Q000627:therapeutic use*; D007249:Inflammation; D015850:Interleukin-6 / Q000097:blood; D018950:Lipopolysaccharide Receptors / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D004731:Endotoxins; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019382:Human Growth Hormone