[Characterization and expression profiling of a novel cereal cyst nematode resistance gene analog in wheat].
Based on the conserved regions of known resistance genes, a NBS-LRR type CCN resistance gene analog was isolated from the CCN resistant E-10 near isogenic lines (NILs) of wheat, designated as CreZ (GenBank Acc. N: EU327996). It contained a complete ORF that was 2775 bp in length and encoded 924 amino acids. Sequence comparison indicated that it shared 92% nucleotide and 87% amino acid identity with those of the known CCN-resistance gene Cre3 and had similar characteristic conserved motifs as those in other established NBS-LRR disease resistance genes. The expression profiling of CreZ indicated that it was specifically expressed in the roots of resistant plants and real-time PCR analysis demonstrated that expression levels drastically increased when the plants were inoculated with cereal cyst nematodes. It could be inferred, then, that CreZ belongs to the NBS-LRR resistance gene family and is a candidate gene for potential resistance to the cereal cyst nematode.
Zhai
X G
XG
Zhao
T
T
Liu
Y H
YH
Long
H
H
Deng
G B
GB
Pan
Z F
ZF
Yu
M Q
MQ
D000818:Animals; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D009348:Nematoda; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D018515:Plant Leaves / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*
D010940:Plant Proteins