title: Assignment of the SLA alleles and reproductive potential of selective breeding Duroc pig lines.
abstract: BACKGROUND
  Pigs with defined swine leukocyte antigen (SLA) haplotypes and their detailed information are useful for transplantation and immunological studies. We developed two herds of SLA homozygous Duroc pigs with novel SLA haplotypes and characterized their reproductive potential.
  
  
  METHODS
  For selective inbreeding, a pair of Duroc pigs was chosen as initial breeders, and substantial breeding within progenies was carried out for eight generations. In the selective breeding Duroc pigs, SLA haplotypes were assigned by nucleotide sequence determination of reverse transcription polymerase chain reaction (RT-PCR) products of three SLA classical class I genes and two class II genes. Based on this sequence information, we developed a rapid and simple SLA class II DNA typing method by polymerase chain reaction-sequence specific primer (PCR-SSP) technique. As a complementary method for the characterization of the SLA haplotypes, genetic polymorphisms of 36 microsatellite (MS) markers within the SLA region were also analyzed in the selective breeding pigs with SLA homozygous/heterozygous haplotypes.
  
  
  RESULTS
  Among the selective breeding pigs from the third to fifth generations, only two SLA haplotypes were identified by the RT-PCR based SLA typing method; Hp-27.30 (SLA-1*08an03, SLA-1*06an04, SLA-2*0102, SLA-3*0101 DRB1*1101 and DQB1*0503) and Hp-60.13 (SLA-1*an02, SLA-2*1002, SLA-3*0502, DRB1*0403 and DQB1*0303). In these two SLA haplotypes, two class I haplotypes, Hp-27.0 and Hp-60.0, are novel. Furthermore, two class II haplotypes, Hp-0.30 and Hp-0.13, which were previously reported in Korean native pigs and pigs of Hanford breed, respectively, were also assigned by a simple assay using a PCR-SSP technique in the entire selective breeding stock. Moreover, two haplotype specific MS patterns were observed across the entire SLA region in the selective breeding (homozygous/heterozygous) pigs. No morphological abnormalities were observed in selective breeding pigs. The theoretical inbreeding coefficient at the eighth generation was 78.5%. In all generations of selective breeding pigs, litter sizes were comparable and weaning weights from the fifth to eighth generation produced progenies significantly lighter (P < 0.01) than those in the non-selective breeding pigs.
  
  
  CONCLUSIONS
  We established and characterized SLA homozygous Duroc herds with two kinds of haplotypes that can be used as a new resource for transplantation and other biomedical studies.
authors.affiliation: Department of Clinical Veterinary Medicine, United Graduate School of Veterinary Science, Gifu University, Gifu, Japan.
authors.forename: Ok Kar
authors.initials: OK
authors.lastname: Soe
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ohba
authors.forename: Noriaki
authors.initials: N
authors.lastname: Imaeda
authors.forename: Naohito
authors.initials: N
authors.lastname: Nishii
authors.forename: Masaki
authors.initials: M
authors.lastname: Takasu
authors.forename: Gou
authors.initials: G
authors.lastname: Yoshioka
authors.forename: Hisako
authors.initials: H
authors.lastname: Kawata
authors.forename: Atsuko
authors.initials: A
authors.lastname: Shigenari
authors.forename: Hirohide
authors.initials: H
authors.lastname: Uenishi
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
authors.forename: Asako
authors.initials: A
authors.lastname: Ando
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kitagawa
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D005260:Female; D006239:Haplotypes / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology*; D000949:Histocompatibility Antigens Class II; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D012098:Reproduction / Q000235:genetics* / Q000276:immunology*; D013552:Swine / Q000145:classification / Q000235:genetics* / Q000276:immunology*
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen