title: Notch signaling confers antigen-presenting cell functions on mast cells.
abstract: BACKGROUND
  Notch signaling is involved in cell fate determination along with the development of the immune system. However, very little is known about the role for Notch signaling in mast cells.
  
  
  OBJECTIVE
  We investigated the role of Notch signaling in mast cell functions.
  
  
  METHODS
  After mouse bone marrow-derived mast cells (BMMCs) or peritoneal mast cells (PMCs) were cocultured with mouse Notch ligand-expressing chinese hamster ovary cells for 5 days, we examined the mast cell surface expressions of MHC-II molecules and OX40 ligand (OX40L), Fc epsilon RI-mediated cytokine production, and the effects of the mast cells on proliferation and differentiation of naive CD4(+) T cells in vitro.
  
  
  RESULTS
  We showed that BMMCs and PMCs constitutively expressed Notch1 and Notch2 proteins on the cell surface. We also found that Delta-like 1 (Dll1)/Notch signaling induced the expression of MHC-II and upregulated the expression level of OX40L on the surface of the mast cells. Dll1/Notch signaling augmented Fc epsilon RI-mediated IL-4, IL-6, IL-13, and TNF production by BMMCs. Dll1-stimulated MHC-II(+)OX40L(high) BMMCs promoted proliferation of naive CD4(+) T cells and their differentiation into T(H)2 cells producing IL-4, IL-5, IL-10, and IL-13.
  
  
  CONCLUSIONS
  Dll1/Notch signaling confers the functions as an antigen-presenting cell on mast cells, which preferentially induce the differentiation of T(H)2.
authors.affiliation: Atopy (Allergy) Research Center, Juntendo University School of Medicine, Tokyo, Japan. nbnakano@med.juntendo.ac.jp
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nakano
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Akemi
authors.initials: A
authors.lastname: Koyanagi
authors.forename: Hisaya
authors.initials: H
authors.lastname: Akiba
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000938:Antigen-Presenting Cells; D016466:CHO Cells; D002135:Calcium-Binding Proteins; D049109:Cell Proliferation; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000276:immunology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053278:OX40 Ligand; D051881:Receptor, Notch1 / Q000235:genetics / Q000276:immunology*; D051883:Receptor, Notch2 / Q000235:genetics / Q000276:immunology*; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology; D048069:Tumor Necrosis Factors / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D002135:Calcium-Binding Proteins; D016207:Cytokines; C512925:Dlk1 protein, mouse; D000949:Histocompatibility Antigens Class II; D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse; D053278:OX40 Ligand; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D017455:Receptors, IgE; C504586:Tnfsf4 protein, mouse; D048069:Tumor Necrosis Factors