title: Survival and growth of Cronobacter species (Enterobacter sakazakii) in wheat-based infant follow-on formulas.
abstract: OBJECTIVE
  To determine the survival and growth characteristics of Cronobacter species (Enterobacter sakazakii) in infant wheat-based formulas reconstituted with water, milk, grape juice or apple juice during storage.
  
  
  RESULTS
  Infant wheat-based formulas were reconstituted with water, ultra high temperature milk, pasteurized grape or apple juices. The reconstituted formulas were inoculated with Cronobacter sakazakii and Cronobacter muytjensii and stored at 4, 25 or 37 degrees C for up to 24 h. At 25 and 37 degrees C, Cronobacter grew more (>5 log(10)) in formulas reconstituted with water or milk than those prepared with grape or apple juices (c. 2-3 log(10)). The organism persisted, but did not grow in any formulas stored at 4 degrees C. Formulas reconstituted with water and milk decreased from pH 6.0 to 4.8-5.0 after 24 h, whereas the pH of the formulas reconstituted with fruit juices remained at their initial pH values, c. pH 4.8-5.0.
  
  
  CONCLUSIONS
  Cronobacter sakazakii and C. muytjensii can grow in reconstituted wheat-based formulas. If not immediately consumed, these formulas should be stored at refrigeration temperatures to reduce the risk of infant infection.
  
  
  CONCLUSIONS
  The results of this study will be of use to regulatory agencies and infant formula producers to recommend storage conditions that reduce the growth of Cronobacter in infant wheat-based formulas.
authors.affiliation: Faculty of Agriculture, Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid, Jordan. tosaili@just.edu.jo
authors.forename: T M
authors.initials: TM
authors.lastname: Osaili
authors.forename: R R
authors.initials: RR
authors.lastname: Shaker
authors.forename: M M
authors.initials: MM
authors.lastname: Ayyash
authors.forename: A A
authors.initials: AA
authors.lastname: Al-Nabulsi
authors.forename: S J
authors.initials: SJ
authors.lastname: Forsythe
mesh_terms: D000818:Animals; D001628:Beverages; D015169:Colony Count, Microbial; D044083:Cronobacter sakazakii / Q000254:growth & development* / Q000302:isolation & purification*; D005511:Food Handling / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D007231:Infant, Newborn; D027845:Malus; D008892:Milk; D013696:Temperature; D014908:Triticum / Q000382:microbiology*; D027843:Vitis; D014867:Water
chemical_list: D014867:Water