Standardization of retention time data for AMT tag proteomics database generation.
The combination of liquid chromatography (LC) with mass spectrometry (MS) has become a mainstream proteome analysis strategy. In LC-MS, measured masses possess their "universal" scale derived from atomic mass tables. In contrast, the observed LC retention times (RT) are not tied to a conventional time scale, and depend on experimental conditions. However, RT data, being explicitly orthogonal to MS, offer relevant information for proteome characterization. We present here a strategy for peptides RT data standardization, based on the generation of a standard scale using retention prediction models, which enables sharing of identification databases in the context of multi-laboratory research.
Tarasova
I A
IA
Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Guryca
V
V
Pridatchenko
M L
ML
Gorshkov
A V
AV
Kieffer-Jaquinod
S
S
Evreinov
V V
VV
Masselon
C D
CD
Gorshkov
M V
MV
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030562:Databases, Protein; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry; D040901:Proteomics
D011506:Proteins