Genetic transformation of Candida albicans.
Genetic transformation is the primary method of genetic manipulation of Candida albicans. The lack of a complete sexual cycle prevents application of classical genetic analyses. However, transformation permits introduction into the genome of a wide variety of defined mutations including deletions, insertions, and fusions. Although several methods of transformation are available, the lithium-cation-induced transformation method described here is the most commonly used.
Ramon
Ana M
AM
Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC, USA.
Fonzi
William A
WA
D002176:Candida albicans / Q000235:genetics*; D005821:Genetic Techniques; D014170:Transformation, Genetic