title: Immobilized metal affinity electrophoresis: a novel method of capturing phosphoproteins by electrophoresis.
abstract: An immobilized metal affinity electrophoresis (IMAEP) method is described here. In this method, metal ions are immobilized in a native polyacrylamide gel to capture phosphoproteins. The capture of phosphoproteins by IMAEP is demonstrated with immobilized metals like iron, aluminum, manganese, or titanium. In the case studies, phosphoproteins alpha-casein, beta-casein, and phosvitin are successfully extracted from a protein mixture by IMAEP.
authors.affiliation: Protein Research Laboratory, Research Resources Center, University of Illinois at Chicago, Chicago, IL 60612-3748, USA. boblee@uic.edu
authors.forename: Bao-Shiang
authors.initials: BS
authors.lastname: Lee
authors.forename: G D
authors.initials: GD
authors.lastname: Lasanthi
authors.forename: P
authors.initials: P
authors.lastname: Jayathilaka
authors.forename: Jin-Sheng
authors.initials: JS
authors.lastname: Huang
authors.forename: Shalini
authors.initials: S
authors.lastname: Gupta
mesh_terms: D000345:Affinity Labels; D001709:Biotechnology; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008670:Metals; D010750:Phosphoproteins / Q000302:isolation & purification*; D010774:Phosvitin / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000345:Affinity Labels; D002364:Caseins; D008670:Metals; D010750:Phosphoproteins; D010774:Phosvitin