Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology.
Unlike transplantation of other solid organs, vascularized mouse lung transplantation has only recently been developed. In this protocol, we describe a detailed method for performing a vascularized and aerated mouse orthotopic lung transplant, which to date represents the most physiological mouse model of lung transplantation. The procedure is divided into two separate portions consisting of donor harvest followed by implantation using the cuff technique for bronchovascular anastomoses. After a training period spanning several months, the procedure can be successfully mastered and, in experienced hands, requires approximately 90 min to perform. After an initial learning curve, perioperative survival is close to 100%. As the donor hematopoietic cells in the transplanted lung are replaced by those of the host over time, thereby creating a 'chimeric lung,' this model represents a novel research tool for the study of transplantation biology as well as other disease processes, such as malignancies.
Krupnick
Alexander S
AS
Department of Surgery, Washington University School of Medicine, Campus Box 8234, 660 South Euclid Avenue, St. Louis, Missouri 63110, USA. krupnicka@wudosis.wustl.edu
Lin
Xue
X
Li
Wenjun
W
Okazaki
Mikio
M
Lai
Jiaming
J
Sugimoto
Seiichiro
S
Richardson
Steven B
SB
Kornfeld
Christopher G
CG
Garbow
Joel R
JR
Patterson
G Alexander
GA
Gelman
Andrew E
AE
Kreisel
Daniel
D
D000818:Animals; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D008168:Lung / Q000098:blood supply*; D016040:Lung Transplantation / Q000379:methods*; D051379:Mice; D023421:Models, Animal