Immuno and lectin histochemistry for renal light microscopy.
Histochemistry is a basic technique in the analysis of cell and tissue biology. Advances in staining techniques have provided the resolving power necessary to study complex organ structures such as the kidney. In this chapter we detail standard histochemical techniques used in our laboratory to localise antigens and carbohydrates with immuno and lectin binding, respectively. Our focus is on immunohistochemistry with fixed paraffin-embedded tissue sections, and its application in the research laboratory.
Hewitson
Tim D
TD
Department of Nephrology, The Royal Melbourne Hospital and Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia.
Grimwood
Lauren
L
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D007668:Kidney / Q000166:cytology*; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
D000906:Antibodies; D037102:Lectins