title: Genetic transformation of Candida albicans.
abstract: Genetic transformation is the primary method of genetic manipulation of Candida albicans. The lack of a complete sexual cycle prevents application of classical genetic analyses. However, transformation permits introduction into the genome of a wide variety of defined mutations including deletions, insertions, and fusions. Although several methods of transformation are available, the lithium-cation-induced transformation method described here is the most commonly used.
authors.affiliation: Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC, USA.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Ramon
authors.forename: William A
authors.initials: WA
authors.lastname: Fonzi
mesh_terms: D002176:Candida albicans / Q000235:genetics*; D005821:Genetic Techniques; D014170:Transformation, Genetic