title: Draxin, a repulsive guidance protein for spinal cord and forebrain commissures.
abstract: Axon guidance proteins are critical for the correct wiring of the nervous system during development. Several axon guidance cues and their family members have been well characterized. More unidentified axon guidance cues are assumed to participate in the formation of the extremely complex nervous system. We identified a secreted protein, draxin, that shares no homology with known guidance cues. Draxin inhibited or repelled neurite outgrowth from dorsal spinal cord and cortical explants in vitro. Ectopically expressed draxin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo. draxin knockout mice showed defasciculation of spinal cord commissural axons and absence of all forebrain commissures. Thus, draxin is a previously unknown chemorepulsive axon guidance molecule required for the development of spinal cord and forebrain commissures.
authors.affiliation: Division of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
authors.forename: Shahidul M
authors.initials: SM
authors.lastname: Islam
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shinmyo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Okafuji
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Su
authors.forename: Iftekhar Bin
authors.initials: IB
authors.lastname: Naser
authors.forename: Giasuddin
authors.initials: G
authors.lastname: Ahmed
authors.forename: Sanbing
authors.initials: S
authors.lastname: Zhang
authors.forename: Sandy
authors.initials: S
authors.lastname: Chen
authors.forename: Kunimasa
authors.initials: K
authors.lastname: Ohta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.forename: Satomi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nishinakamura
authors.forename: Toshio
authors.initials: T
authors.lastname: Terashima
authors.forename: Toshio
authors.initials: T
authors.lastname: Kitamura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000502:physiology*; D019556:COS Cells; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D003337:Corpus Callosum / Q000196:embryology / Q000378:metabolism; D018274:Electroporation; D020439:Growth Cones / Q000378:metabolism / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016501:Neurites / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis; D009457:Neuroglia / Q000378:metabolism; D016548:Prosencephalon / Q000002:abnormalities / Q000196:embryology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; C534831:draxin protein, mouse