title: Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage.
abstract: 3,4-Methylenedioxymethamphetamine (MDMA, Ecstasy) is a psychoactive drug with abuse liability and neurotoxic potential. Specimen preparation of a recently presented LC-MS assay with electrospray ionization for quantifying MDMA and its main metabolites in squirrel monkey plasma was modified to include acidic hydrolysis to obtain total 3,4-dihydroxymethamphetamine and 4-hydroxy-3-methoxy-methamphetamine. Method re-validation for squirrel monkey plasma and full validation for human plasma showed selectivity for all analytes. Recoveries were > or = 71.0%. Changed specimen preparation or matrix did not affect accuracy or precision. No instability was observed after repeated freezing or in processed samples. Plasma MDMA and metabolites quantification, derived pharmacokinetic and toxicokinetic data and neurotoxicity research will benefit from this validated method.
authors.affiliation: Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, D-66421 Homburg (Saar), Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Mueller
authors.forename: Frank T
authors.initials: FT
authors.lastname: Peters
authors.forename: Marilyn A
authors.initials: MA
authors.lastname: Huestis
authors.forename: George A
authors.initials: GA
authors.lastname: Ricaurte
authors.forename: Hans H
authors.initials: HH
authors.lastname: Maurer
mesh_terms: D015104:3,4-Methylenedioxyamphetamine / Q000097:blood / Q000302:isolation & purification; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003846:Deoxyepinephrine / Q000031:analogs & derivatives / Q000097:blood / Q000302:isolation & purification; D053593:Forensic Toxicology; D006213:Hallucinogens / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D006868:Hydrolysis; D008694:Methamphetamine / Q000031:analogs & derivatives / Q000097:blood / Q000302:isolation & purification; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000097:blood / Q000302:isolation & purification; D012453:Saimiri; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D006213:Hallucinogens; C079646:4-hydroxy-3-methoxymethamphetamine; C069512:alpha-methylepinine; D008694:Methamphetamine; D015104:3,4-Methylenedioxyamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine; D003846:Deoxyepinephrine