title: Oxidative DNA damage in retinopathy of prematurity.
abstract: OBJECTIVE
  This study examines the levels of oxidative damage in patients with retinopathy of prematurity (ROP).
  
  
  METHODS
  Fifty patients were recruited with a birthweight below 1500 g or gestational age below 32 weeks. The cases were classified into those who developed ROP (n=25) and those without ROP (n=25). The authors obtained blood and urine samples from each infant, for measuring 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels, at the time of the first examination at 4-6 postnatal weeks.
  
  
  RESULTS
  A significant difference was observed in leukocyte and urine 8-OHdG levels in patients with ROP compared to those without ROP (p<0.001 for both). Similarly, a significant difference was observed in plasma and urine MDA levels in patients with ROP compared to those without ROP (p<0.001 for both). In addition, significant correlations were found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r=0.859, p<0.001), and between levels of urine 8-OHdG excretion and urine MDA (r=0.563, p<0.001).
  
  
  CONCLUSIONS
  8-OHdG in leukocyte DNA and urine levels in premature infants can be useful as an indicator for ROP screening.
authors.affiliation: Department of Ophthalmology Medical Faculty, Ataturk University, Erzurum, Turkey. orhanates@atauni.edu.tr
authors.forename: O
authors.initials: O
authors.lastname: Ates
authors.forename: H H
authors.initials: HH
authors.lastname: Alp
authors.forename: I
authors.initials: I
authors.lastname: Caner
authors.forename: A
authors.initials: A
authors.lastname: Yildirim
authors.forename: A
authors.initials: A
authors.lastname: Tastekin
authors.forename: I
authors.initials: I
authors.lastname: Kocer
authors.forename: O
authors.initials: O
authors.lastname: Baykal
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001724:Birth Weight; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005865:Gestational Age; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight; D007962:Leukocytes / Q000378:metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood / Q000652:urine; D015997:Neonatal Screening / Q000379:methods; D018384:Oxidative Stress; D012178:Retinopathy of Prematurity / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine