title: Prevalence of human papillomavirus genotypes in women from a rural region of Puebla, Mexico.
abstract: OBJECTIVE
  To determine the prevalence of human papillomavirus (HPV) genotypes 6, 11, 16, 18, and 31 in Mexican women living in rural areas of Puebla, Mexico and to evaluate risk factors associated with cervical neoplasm in this population.
  
  
  METHODS
  A cross-sectional study was conducted in 326 women at the General Hospital of Metepec, Puebla. Cervical samples were obtained using a cytobrush and tested for HPV genotypes by PCR assays using type-specific primers. A questionnaire was completed regarding gynecological, obstetric, and sexual behavior of the patients.
  
  
  RESULTS
  The overall prevalence of HPV infection was 25.4%, with two peaks of higher HPV prevalence in those aged 18-24 and 55-64 years. The individual genotype prevalences were: 9.6% HPV6, 4.8% HPV11, 54.2% HPV16, 37.3% HPV18, and 9.6% HPV31. Number of pregnancies was the most important risk factor associated with cervical cancer.
  
  
  CONCLUSIONS
  HPV16 was the most common type found in all cervical lesions. Genotype 16 or 18 was detected in patients with a diagnosis of cervical cancer. We found two peaks of age-specific HPV prevalence similar to findings reported worldwide.
authors.affiliation: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Federal Atlixco-Metepec, 74360 Metepec, Puebla, Mexico.
authors.forename: Noé
authors.initials: N
authors.lastname: Velázquez-Márquez
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Paredes-Tello
authors.forename: Héctor
authors.initials: H
authors.lastname: Pérez-Terrón
authors.forename: Gerardo
authors.initials: G
authors.lastname: Santos-López
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: Verónica
authors.initials: V
authors.lastname: Vallejo-Ruiz
mesh_terms: D000293:Adolescent; D000328:Adult; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002584:Cervix Uteri / Q000821:virology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012307:Risk Factors; D012424:Rural Population; D011795:Surveys and Questionnaires; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology; D014626:Vaginal Smears; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology
chemical_list: D004279:DNA, Viral