[Study of cbfalpha1 on the expression of extracellular matrix proteins in dental papilla cells induced by BMP-2 in vitro].
OBJECTIVE
To evaluate the function of cbfalpha1 on BMP-2 signaling to extracellular matrix proteins in dental papilla cells in vitro.


METHODS
RT-PCR and Western blot were performed to detect the expression of ALP, OC, ON, OPN, BSP, DMP-1 and DSPP in cultured dental papilla cells induced by 200ng/mL BMP-2 and/or down-regulated by cbfalpha1 antisense technology, the results were analysed with SPSS 11.0 software package.


RESULTS
We found that the amount of ALP and OC and the expression of OPN, BSP and ON were upregulated significantly after the cells were treated with BMP-2. After transfected with antisense cbfalpha1, the cells downregulated the expression of ALP, OC, OPN and BSP significantly(P<0.01).


CONCLUSIONS
As a kind of transcription factor, cbfalpha1 could be an important tache in the BMP-2 signal networks controlling cells differentiation and mineralization.
Yu
Qing
Q
Department of Operative Dentistry and Endodontics, Fourth Military Medical University, Xi'an 710032, China. yuqing@fmmu.edu.cn
Zhu
Qin-ling
QL
Sun
Han-tang
HT
Tian
Yu
Y
He
Wen-xi
WX
Xiao
Ming-zhen
MZ
D000469:Alkaline Phosphatase; D055396:Bone Morphogenetic Protein 2 / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D050657:Core Binding Factor alpha Subunits / Q000502:physiology*; D003771:Dental Papilla / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D050657:Core Binding Factor alpha Subunits; D016326:Extracellular Matrix Proteins; C406688:core binding factor alpha; D000469:Alkaline Phosphatase