Metabolic reprogramming of periwinkle plant culture.
We transformed an alkaloid biosynthetic gene with reengineered substrate specificity into Catharanthus roseus. The resulting transgenic plant cell culture produced a variety of unnatural alkaloid compounds when cocultured with simple, achiral, commercially available precursors that the reengineered enzyme was designed to accept. This work demonstrates the power of genetic engineering to retailor the structures of complex alkaloid natural products in plant culture.
Runguphan
Weerawat
W
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue 18-592, Cambridge, Massachusetts 02139, USA.
O'Connor
Sarah E
SE
D030008:Catharanthus / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D026121:Indole Alkaloids / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism
D026121:Indole Alkaloids