The suppression of hypersensitivity by ocular-induced CD8(+) T cells requires compatibility in the Qa-1 haplotype.
The injection of antigen into the anterior chamber (AC, intracameral injection) of a murine eye induces the generation of splenic CD8(+) regulatory T cells (AC-SPL cells) that effect the antigen-specific suppression of a delayed-type hypersensitivity (DTH) reaction. Here we show (i) for the first time that the local antigen-specific suppression of DTH-induced swelling in immunized mice either by an intracameral injection of antigen or by the direct injection of CD8(+) AC-SPL cells into an antigen-challenged site is associated with an absence of infiltrated mononuclear cells, (ii) that the local antigen-specific suppression of the DTH reaction by CD8(+) AC-SPL cells requires compatibility between the Qa-1 but not H2 antigen haplotype of the immunized recipient and the injected AC-SPL regulatory T cells, (iii) that the suppression of the DTH reaction by CD8(+) AC-SPL cells requires the expression of Qa-1 but not H2 antigens and is not due to bystander suppression.
Cone
Robert E
RE
Department of Immunology, Connecticut Lions Vision Center, University of Connecticut Health Center, Farmington, CT 06030-3105, USA. Cone@UCHC.EDU
Chattopadhyay
Subhasis
S
Sharafieh
Roshanak
R
Lemire
Yen
Y
O'Rourke
James
J
D000818:Animals; D000867:Anterior Chamber; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000473:pathology / Q000628:therapy*; D007108:Immune Tolerance / Q000235:genetics; D007165:Immunosuppression Therapy; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D010851:Picrates / Q000276:immunology / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; D010851:Picrates; C032907:Q surface antigens; D010047:Ovalbumin; C005858:picric acid