[Immuno-screening of Schistosoma japonicum cercariae cDNA library by the sera of anti-soluble cercariae 66 to approximately 68 kD antigens].
OBJECTIVE
To obtain the coding genes related to Schistosoma japonicum (Sj) cercariae 66 to approximately 68 kD antigens,and to provide antigens for diagnosis and vaccine of schistosomiasis.


METHODS
Sj cercariae cDNA library was screened using the monospecific anti-sera of rabbit against soluble cercariae 66 to approximately 68 kD antigens as probes.The inserted cDNA fragments of the positive clones were amplified with PCR and identified by agarose gel electrophoresis. Four strong positive clones were further sequenced and analyzed through the internet NCBI/BLAST software.


RESULTS
Twenty-one positive clones were obtained, 10 of which revealed a single band (0.5 to approximately 3.0 kb).The 4 strong positive clones showed high identity to SJCHGC05187,SJCHGC05173,SJCHGC06989, and SJCHGC01894 at the nucleotide level.


CONCLUSIONS
Four coding genes related with Sj antigens are obtained.
Qin
Yong-Hua
YH
Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China. wsp4373383@126.com
Zhou
Shuai-Feng
SF
Wang
Shi-Ping
SP
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D058487:Cercaria / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D007106:Immune Sera / Q000276:immunology*; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D007106:Immune Sera; C482321:Sj-MA protein, Schistosoma japonica