title: Identification and functional characterization of a novel cytidine deaminase in a gastropod abalone, Haliotis diversicolor supertexta.
abstract: Cytidine deaminase (CDA, also designated CDD) is a zinc-dependent enzyme involved in the pyrimidine salvage pathways and becoming very important in anticancer and antiviral therapy. Here we report the identification and characterization of a CDA homologue in abalone, which we named ab-CDA. The analysis of the amino acids sequence revealed that the ab-CDA shares conserved signature motifs and belongs to homotetrameric class of CDA family. Real-time PCR analysis indicated that the ab-CDA was ubiquitously expressed in various tissues of abalone and relatively higher expressed in hemocyte. Significant up-regulation of ab-CDA was also observed after LPS or Poly I: C challenge. The biological activity of ab-CDA was identified by spectrophotometry analysis and the intracellular localization displayed that ab-CDA was largely concentrated in the cytoplasm and partially in the nuclei. These results strongly suggest that ab-CDA is a CDA homologue and it is involved in the immune response of gastropod abalone.
authors.affiliation: Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, PR China.
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Wu
authors.forename: Baojian
authors.initials: B
authors.lastname: Zhu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003564:Cytidine Deaminase / Q000378:metabolism*; D049851:Gastropoda / Q000145:classification / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011994:Recombinant Proteins; D003564:Cytidine Deaminase