title: Quinolone resistance in Neisseria gonorrhoeae: rapid genotyping of quinolone resistance-determining regions in gyrA and parC genes by melting curve analysis predicts susceptibility.
abstract: We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. Our assay clearly detects all mutated QRDRs and allows the identification of common genotypes, whether the QRDRs contain single or double mutations, providing valuable epidemiological tools. When this method is used in conjunction with similar assays and in vitro analyses, essential antibiotic resistance surveillance can be performed for public health purposes.
authors.affiliation: Laboratoire de Recherche en Bactériologie, Institut Pasteur de Nouvelle-Calédonie, Nouméa Cedex, New Caledonia.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Vernel-Pauillac
authors.forename: Tiffany R
authors.initials: TR
authors.lastname: Hogan
authors.forename: John W
authors.initials: JW
authors.lastname: Tapsall
authors.forename: Cyrille
authors.initials: C
authors.lastname: Goarant
mesh_terms: D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004198:Disease Susceptibility; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D015363:Quinolones / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D004269:DNA, Bacterial; D015363:Quinolones; D027081:DNA Gyrase