Genetics and proteomics of Aeromonas salmonicida lipopolysaccharide core biosynthesis.
Comparison between the lipopolysaccharide (LPS) core structures of Aeromonas salmonicida subsp. salmonicida A450 and Aeromonas hydrophila AH-3 shows great similarity in the inner LPS core and part of the outer LPS core but some differences in the distal part of the outer LPS core (residues ld-Hep, d-Gal, and d-GalNAc). The three genomic regions encoding LPS core biosynthetic genes in A. salmonicida A450, of which regions 2 and 3 have genes identical to those of A. hydrophila AH-3, were fully sequenced. A. salmonicida A450 region 1 showed seven genes: three identical to those of A. hydrophila AH-3, three similar but not identical to those of A. hydrophila AH-3, and one without any homology to any well-characterized gene. A. salmonicida A450 mutants with alterations in the genes that were not identical to those of A. hydrophila AH-3 were constructed, and their LPS core structures were fully elucidated. At the same time, all the A. salmonicida A450 genes identical to those of A. hydrophila AH-3 were used to complement the previously obtained A. hydrophila AH-3 mutants for each of these genes. Combining the gene sequence and complementation test data with the structural data and phenotypic characterization of the mutant LPSs enabled a presumptive assignment of all LPS core biosynthesis gene functions in A. salmonicida A450. Furthermore, hybridization studies with internal probes for the A. salmonicida-specific genes using different A. salmonicida strains (strains of different subspecies or atypical strains) showed a unique or prevalent LPS core type, which is the one fully characterized for A. salmonicida A450.
Jimenez
Natalia
N
Departamento de Microbiología, Universidad Barcelona, Spain.
Lacasta
Anna
A
Vilches
Silvia
S
Reyes
Mercé
M
Vazquez
Judit
J
Aquillini
Eleonora
E
Merino
Susana
S
Regué
Miguel
M
Tomás
Juan M
JM
D016980:Aeromonas hydrophila / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D048409:Aeromonas salmonicida / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002240:Carbohydrate Sequence; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D040901:Proteomics
D001426:Bacterial Proteins; D008070:Lipopolysaccharides