title: [Characterization of first sorbitol-fermenting shiga toxin-producing Escherichia coli O157:H- strain isolated in Poland].
abstract: Sorbitol-fermenting shiga toxin-producing E. coli O157:H- strains have emerged as a cause of human disease in many European and non-European countries. The role of SF VTEC O157:H- in the etiology of pediatric HUS and diarrhea is significant. We characterized the first SF VTEC O157:H- strain isolated from 9 year old patient in Poland. Strain possessed many traits characteristics for SF VTEC O157:H-. It fermented sorbitol after overnight incubation and produced beta-glucuronidase. It possessed the stx2, eae-gamma, EhlyA and sfpA genes and did not harbour plasmid-encoded katP and espP genes. Motility was not expressed but the strain possessed the chromosomal fliC locus for H7 antigen. The spread of SF VTEC O157:H- strains demonstrates the need for appropriate procedures for their microbiological diagnosis in Poland.
authors.affiliation: Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego, Państwowego Zakładu Higieny w Warszawie.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jakubczak
authors.forename: Jolanta
authors.initials: J
authors.lastname: Szych
authors.forename: Kamil
authors.initials: K
authors.lastname: Januszkiewicz
mesh_terms: D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D011044:Poland; D022621:Shiga Toxin / Q000096:biosynthesis*; D013012:Sorbitol / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D013012:Sorbitol; D022621:Shiga Toxin