Adhesive properties of environmental Vibrio alginolyticus strains to biotic and abiotic surfaces.
The ability of Vibrio alginolyticus strains isolated from a bathing and fishing area (Khenis, Centre of Tunisia) to adhere to both biotic and abiotic surfaces was evaluated in the present work. The biochemical, physiological and enzymatic activities of all strains was also investigated. Three morphotypes of V. alginolyticus were obtained on Congo red agar and only 14 strains produced black colonies. The majority of strains were able to degrade the skin mucus of both Sparus aurata and Dicentrarchus labrax fishes while the fish mucus preparation of these two specimens exhibits a high level of anti-V. alginolyticus strains. Adhesive properties were observed in 37.5% of the analyzed V. alginolyticus strains to Hep-2 cells and 50% to Caco-2 cells. All strains were able to form a purple pellicule on glass tube when they were stained with Crystal violet. Fifteen percent of V. alginolyticus strains (16/32) were strongly adhesive to polystyrene with a values ranging from 3.04 to 18.25 at 595 nm and only four strains were weak biofilm forming. V. alginolyticus bacterium possess a strong adhesive power to both biotic and inertes surfaces. These proprieties may allow to these strains to persist in this biotope in planctonic state or attached to both biotic and abiotic surfaces.
Snoussi
Mejdi
M
Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Department of Microbiology, Faculty of Pharmacy, Monastir, Tunisia. snmejdi@yahoo.fr
Noumi
Emira
E
Cheriaa
Jihane
J
Usai
Donatella
D
Sechi
Leonardo Antonio
LA
Zanetti
Stefania
S
Bakhrouf
Amina
A
D000818:Animals; D001422:Bacterial Adhesion; D001492:Bass; D018441:Biofilms / Q000254:growth & development; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D004777:Environment; D005898:Glass; D006801:Humans; D009093:Mucus / Q000382:microbiology; D011137:Polystyrenes; D021541:Sea Bream; D044149:Vibrio alginolyticus / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*
D011137:Polystyrenes