Effects of DNA damage induced by UV irradiation on gene expression in the protozoan parasite Entamoeba histolytica.
Previously, we provided evidence for the role of E. histolytica RAD52 epistasis group genes and the EhRAD51 recombinase in DNA damage response. To identify other genes participating in DNA repair in this protozoan parasite, here we analyzed the transcriptional response to genetic damage induced by ultraviolet light (UV) using cDNA microarrays. We found that 11.6% (350 ORFs) and 17.2% (522 ORFs) of genes were modulated at 5 min and 3h after UV irradiation, respectively. Most genes were less than 2-fold changed evidencing a weak transcriptional activation. The genes encoding so-called "classical" DNA repair proteins were slightly regulated in trophozoites submitted to UV irradiation. We also observed the over-expression of genes encoding for Fe-S clusters-containing proteins, potentially involved in the stress adaptation in response to DNA damage. Several genes encoding cytoskeleton proteins were repressed suggesting that actin dynamics was impaired after UV irradiation. Our analysis highlights novel genes potentially involved in DNA damage response, and these data will contribute to further elucidation of mechanisms regulating genome integrity in this early branch protozoan.
Weber
Christian
C
Institut Pasteur, Unité Biologie Cellulaire du Parasitisme, Paris, France.
Marchat
Laurence A
LA
Guillen
Nancy
N
López-Camarillo
César
C
D000818:Animals; D004249:DNA Damage; D045643:DNA Repair Enzymes / Q000096:biosynthesis; D016054:DNA, Protozoan / Q000528:radiation effects*; D004748:Entamoeba histolytica / Q000528:radiation effects*; D015870:Gene Expression; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D015800:Protozoan Proteins / Q000096:biosynthesis
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D045643:DNA Repair Enzymes