The effect of electron and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human blood lymphocytes.
The effect of electrons and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human peripheral blood lymphocytes was investigated to understand the relative biological effectiveness (RBE) of electrons compared with gamma rays. Blood samples were irradiated with an 8 MeV pulsed electron beam, at a mean instantaneous dose rate of 2.6 x 10(5) Gy s(-1). Gamma irradiation was carried out at a dose rate of 1.98 Gy min(-1) using (60)Co gamma source. A dose-dependent increase in micronuclei yield was observed. The dose-response relationships for induction of micronuclei fitted well to a linear-quadratic relationship and the coefficients alpha and beta of the dose-response curve were estimated by fitting the data using error-weighted minimum chi (2) method. The RBE of 8 MeV electrons were found to be near unity as compared with gamma rays.
Acharya
Santhosh
S
Microtron Centre, Department of Studies in Physics, Mangalore University, Mangalagangotri, Mangalore 574199, India.
Sanjeev
Ganesh
G
Bhat
N N
NN
Siddappa
K
K
Narayana
Y
Y
D000328:Adult; D002138:Calibration; D003037:Cobalt Radioisotopes; D048749:Cytokinesis / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods; D015233:Models, Statistical; D011874:Radiometry; D012062:Relative Biological Effectiveness; D015203:Reproducibility of Results
D003037:Cobalt Radioisotopes