A new titration system of a novel split-type superconducting magnet NMR spectrometer.
A new titration system for studying protein-ligand interactions has been developed. In this system, the sample solution is circulated in the route formed by an access path in a split superconducting magnet to maintain a constant protein concentration during the titration experiments. A concentration-control procedure for the ligand/protein ratio is devised, and the ligand/protein ratio is well controlled by this apparatus.
Kitagawa
Isao
I
Advanced Research Laboratory, Hitachi, Ltd., Kokubunji, Tokyo 185-8601, Japan. isao.kitagawa.zd@hitachi.com
Tanaka
Hideki
H
Okada
Michiya
M
Kitaguchi
Hitoshi
H
Kohzuma
Takamitsu
T
D004867:Equipment Design; D008024:Ligands; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*; D008280:Magnetics; D010825:Physics / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D017186:Titrimetry / Q000295:instrumentation; D014364:Tryptophan / Q000737:chemistry
D008024:Ligands; D011506:Proteins; D014364:Tryptophan