Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer.
BACKGROUND
We develop a multiplex quantitative real-time PCR for synchronized analysis of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) to investigate relative mtDNA abundance in paired normal and cancerous breast tissues.


METHODS
The amounts of nDNA and mtDNA in 102 tissue samples were quantified for both glyceraldehype-3-phosphodehydrogenase (GAPDH) gene and mtDNA encoded ATPase (MTATP) 8 gene. The average threshold cycle (Ct) number values of the nDNA and mtDNA were used to calculate relative mtDNA content in breast tissues.


RESULTS
The median delta Ct (DeltaCt) and the median mtDNA content for normal and cancerous breast tissues were 6.73 and 2.54, as well as 106.50 and 5.80 (P = 0.000, respectively). The mtDNA content was decreased in 82% of cancerous breast tissues compared with the normal ones. The changes were associated with hormone receptor status.


CONCLUSIONS
Our finding suggests that decreased mtDNA content in breast cancer may have diagnostic and prognostic value for the disease.
Fan
Alex Xiu-Cheng
AX
Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031 Basel, Switzerland.
Radpour
Ramin
R
Haghighi
Mahdi Montazer
MM
Kohler
Corina
C
Xia
Peng
P
Hahn
Sinuhe
S
Holzgreve
Wolfgang
W
Zhong
Xiao Yan
XY
D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology*; D004272:DNA, Mitochondrial / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014018:Tissue Distribution
D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases