title: Microcirculatory alteration in low-grade gastric mucosa-associated lymphoma by Helicobacter heilmannii infection: its relation to vascular endothelial growth factor and cyclooxygenase-2.
abstract: BACKGROUND
  There are clinical reports that Helicobacter heilmannii, as well as Helicobacter pylori, has been clinically reported to cause gastric low-grade mucosa-associated lymphoid tissue-type (MALT) lymphoma, although its precise mechanism remains to be clarified. Thus, the present study was undertaken to elucidate the alteration of the microcirculatory structure and the relation to angiogenetic factors in mice infected with H. heilmannii for 3 and 6 months.
  
  
  METHODS
  Immunohistochemical studies have been performed by FITC-dextran intra-aortic infusion or CD31, vascular endothelial growth factor-A, cyclooxygenase 2 antibodies using our recently established model of gastric mucosa-associated lymphoid tissue-type gastric B-cell lymphoma in C57BL/6 mice.
  
  
  RESULTS
  Increased microcirculatory network was recognized surrounding the MALT lymphoma tissues by both the FITC-dextran infusion method and CD31 immunoreactivity. Vascular endothelial growth factor-A immunoreactivity was recognized within the lymphoma tissues as well as in the marginal area, while cyclooxygenase-2 immunoreactivity was localized in the area surrounding the MALT lymphoma tissues.
  
  
  CONCLUSIONS
  Increased microvascular network as well as enhanced VEGF-A immunoreactivity was shown to be related to expansion of the MALT lymphoma formed by Helicobacter heilmannii infection.
authors.affiliation: Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan. nakamuram@pharm.kitasato-u.ac.jp
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.forename: Somay Yamagata
authors.initials: SY
authors.lastname: Murayama
authors.forename: Chihiro
authors.initials: C
authors.lastname: Aikawa
authors.forename: Yukie
authors.initials: Y
authors.lastname: Sekiya
authors.forename: Kaori
authors.initials: K
authors.lastname: Nishikawa
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Kanji
authors.initials: K
authors.lastname: Tsuchimoto
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000098:blood supply / Q000201:enzymology / Q000382:microbiology*; D016481:Helicobacter Infections / Q000150:complications / Q000382:microbiology* / Q000503:physiopathology; D018247:Helicobacter heilmannii / Q000472:pathogenicity*; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000201:enzymology / Q000382:microbiology* / Q000503:physiopathology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008833:Microcirculation; D009389:Neovascularization, Pathologic / Q000201:enzymology / Q000382:microbiology* / Q000503:physiopathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2