title: Proton-detected solid-state NMR spectroscopy of natural-abundance peptide and protein pharmaceuticals.
abstract: The natural way: A sensitive NMR spectroscopic method is developed to obtain well-resolved two-dimensional spectra ((15)N-(1)H and (13)C-(1)H) for natural-abundance (that is, without the need for isotopic enrichment) large-molecule samples, such as biopharmaceuticals. This method gives structural insights on two lyophilized aprotinin samples and three insulin samples in lyophilized, microcrystalline suspension formulation (red; see picture) and fibril (green) forms.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Donghua H
authors.initials: DH
authors.lastname: Zhou
authors.forename: Gautam
authors.initials: G
authors.lastname: Shah
authors.forename: Charles
authors.initials: C
authors.lastname: Mullen
authors.forename: Dennis
authors.initials: D
authors.lastname: Sandoz
authors.forename: Chad M
authors.initials: CM
authors.lastname: Rienstra
mesh_terms: D007611:Aprotinin / Q000737:chemistry; D005612:Freeze Drying; D006801:Humans; D007328:Insulin / Q000737:chemistry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011522:Protons
chemical_list: D007328:Insulin; D010455:Peptides; D011506:Proteins; D011522:Protons; D007611:Aprotinin