Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma.
A LC/MS/MS method was developed and validated for determination of nobiliside A in rat plasma. Analyses were separated by an Agella Venusil ASB-C18 column and isocratic elution with methanol:water (80:20, v/v) as a mobile phase. A tandem mass spectrometer was used as a detector for quantitative analysis. Calibration curves (R(2)>0.999) in spiked plasma were linear over the concentration range of 50-5000 ng/mL. The overall accuracy of this method was 93-104% for nobiliside A. Within-day and between-day variability was both less than 9% in plasma. The data indicate that the LC/MS/MS method is an effective method for the pharmacokinetics study of nobiliside A in rat plasma.
Guo
Dan
D
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Xiong
Yang
Y
Zhang
Yue
Y
Wu
Zhongbin
Z
Cui
Lili
L
Chen
Jianming
J
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D051381:Rats; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
C515791:3-O-xylopyranosylholosta-7,9(11),22,24-tetraene-3,17-diol; D012503:Saponins