Nephro-protective effect of Kangqianling decoction on chronic renal failure rats.
BACKGROUND
Kangqianling decoction (KQL), the modified formulation of a classical Chinese prescription named Taohongsiwu decoction, was clinically employed to treat renal fibrosis in chronic renal failure.


OBJECTIVE
The present study was designed to examine whether KQL has a protective effect on renal function in association with transforming growth factor-beta (TGF-beta), angiotensin II (Ang II), tumor necrosis factor-alpha (TNF-alpha), nuclear factor-kappaB (NF-kappaB) in rats with 5/6 renal ablation (Nx)-induced chronic renal failure.


RESULTS
In renal function deterioration progression, the high expression of serum creatinine (Scr), 24-h urine protein and systolic blood pressure were markedly (P<0.05 or P<0.01) restored by KQL, respectively, at 4 and 8 weeks. The increasing expressions of renal Ang II (P<0.05), angiotensin II1-receptor (AT1R) (P<0.05), TNF-alpha (P<0.05), NF-kappaB (P<0.001) and urine TGF-beta1 (P<0.05) were reduced by the treatment of KQL. Immunohistochemical study further confirmed the nephro-protective activity of KQL as compared to the control and Sham group.


CONCLUSIONS
The results indicate that KQL is able to protect renal function via ameliorating experimental rat renal failure as found in these renal functional parameters.
He
Liqun
L
Traditional Chinese internal medicine E-institute of Shanghai University (Shanghai TCM University), Nephropathy Department, ShuGuang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Shen
Peicheng
P
Fu
Qiang
Q
Li
Jun
J
Dan
Mo
M
Wang
Xiaoyan
X
Jia
Wei
W
D029971:Angelica sinensis; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000188:drug therapy* / Q000473:pathology; D019684:Magnoliopsida; D016328:NF-kappa B / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism; D027561:Salvia miltiorrhiza; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D031957:Ziziphus
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D011506:Proteins; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; C533464:kangxianling; D000804:Angiotensin II; D003404:Creatinine