Genetic variation in the long terminal repeat associated with the transition of Chinese equine infectious anemia virus from virulence to avirulence.
A highly virulent strain of equine infectious anemia virus (EIAV) lost its fatal virulence but retained the desired antigens during serial passage over 130 generations in leukocytes in vitro. We compared the long terminal repeat (LTR) sequences of the different generations and found that three stable genetic variations occurred in the transcriptional start site, the initial base of TAR, and the pre-mRNA cleavage site at the R-U5 boundary, respectively. These three mutations happened at the inflexion of virus pathogenicity loss; therefore, the function of these mutations needs to be further addressed.
Wei
Lili
L
Harbin Veterinary Research Institute of Chinese Academy of Agriculture Sciences (CAAS), 427 Maduan Street, Harbin, 150001, China.
Fan
Xiujuan
X
Lu
Xiaoling
X
Zhao
Liping
L
Xiang
Wenhua
W
Zhang
Xiaoyan
X
Xue
Fei
F
Shao
Yiming
Y
Shen
Rongxian
R
Wang
Xiaojun
X
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018397:Equidae; D014644:Genetic Variation; D004860:Infectious Anemia Virus, Equine / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D008264:Macrophages / Q000821:virology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D020079:Terminal Repeat Sequences; D014774:Virulence
D012367:RNA, Viral