title: Antioxidant compound from the rhizomes of Kaempferia rotunda L.
abstract: This research aimed to investigate the antioxidant effect from rhizomes of K. rotunda for finding the active compounds by DPPH free radical scavenging activity assay. The chloroform-soluble extract of the rhizomes of K. rotunda showed significant scavenging effect on the on 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radicals (IC50 = 180 microg mL(-1)). Two compounds of the chloroform-soluble extract were isolated and identified. Compound 1, 2'-hydroxy-4,4',6'-trimethoxy-chalcone was found as the active constituent (IC50 = 142 microg mL(-1)). Compound 2, (+)-crotepoxide, was inactive (IC50 = 1516 microg mL(-1)). The structures of compounds 1 and 2 were identified based on the basis of spectral evidence, Mass Spectrophotometry (MS) and 2D-NMR (2 dimension of Nuclear Magnetic Resonance) data including Heteromolecular Multiple Quantum Coherence (HMQC) and Heteromolecular Multiple Bond Correlation (HMBC) and comparison to published values.
authors.affiliation: Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPIPTEK, Serpong 15314, Indonesia.
authors.forename: Puspa D N
authors.initials: PD
authors.lastname: Lotulung
authors.lastname: Minarti
authors.forename: L B S
authors.initials: LB
authors.lastname: Kardono
authors.forename: K
authors.initials: K
authors.lastname: Kawanishi
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D001713:Biphenyl Compounds; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005609:Free Radicals; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010851:Picrates; D027343:Rhizome / Q000378:metabolism; D030521:Zingiberaceae / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D047188:Chalcones; D004852:Epoxy Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; C011828:futoxide; C004931:1,1-diphenyl-2-picrylhydrazyl