title: Wide-bore hydrodynamic chromatography in sub-second range.
abstract: Wide-bore hydrodynamic chromatography (HDC) is a very simple method based on the passage of analytes through an open capillary. As shown in previous papers, an analyte, which diffuses over the entire cross-section of a capillary before it is detected, gives a Gaussian-shaped peak; such an analyte is called diffusive. In contrast, an analyte with poor diffusivity gives an asymmetrical peak with the apex at the half time of the peak of a diffusive analyte. Their diffusivity is determined by the diffusion coefficients, dimensions of a capillary, and a flow rate. A consideration of the diffusivity evaluated on the basis of these variables allows us to realize fast wide-bore HDC separation. Sub-second separation is demonstrated using test analytes, such as fluorescein and polystyrene latexes.
authors.affiliation: Department of Chemistry, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Umehara
authors.forename: Makoto
authors.initials: M
authors.lastname: Harada
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Okada