title: Molecular dynamics calculations suggest a conduction mechanism for the M2 proton channel from influenza A virus.
abstract: The M2 protein of the influenza A virus is activated by low endosomal pH and performs the essential function of proton transfer into the viral interior. The resulting decrease in pH within the virion is essential for the uncoating and further replication of the viral genetic material. The x-ray crystal [Stouffer AL, et al. (2008) Nature 451:596-599] and solution NMR [Schnell JR, Chou JJ (2008) Nature 451:591-595] structures of the transmembrane region of the M2 homo-tetrameric bundle both revealed pores with narrow constrictions at one end, leaving a question as to how protons enter the channel. His-37, which is essential for proton-gating and selective conduction of protons, lies in the pore of the crystallographic and NMR structures. Here, we explore the different protonation states of the His-37 residues of the M2 bundle in a bilayer using molecular dynamics (MD) simulations. When the His-37 residues are neutral, the protein prefers an Open(out)-Closed(in) conformation in which the channel is open to the environment on the outside of the virus but closed to the interior environment of the virus. Diffusion of protons into the channel from the outside of the virus and protonation of His-37 residues in the tetramer stabilizes an oppositely gated Closed(out)-Open(in) conformation. Thus, protons might be conducted through a transporter-like mechanism, in which the protein alternates between Open(out)-Closed(in) and Closed(out)-Open(in) conformations, and His-37 is protonated/deprotonated during each turnover. The transporter-like mechanism is consistent with the known properties of the M2 bundle, including its relatively low rate of proton flux and its strong rectifying behavior.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA. ekta.khurana@yale.edu
authors.forename: Ekta
authors.initials: E
authors.lastname: Khurana
authors.forename: Matteo
authors.initials: M
authors.lastname: Dal Peraro
authors.forename: Russell
authors.initials: R
authors.lastname: DeVane
authors.forename: Satyavani
authors.initials: S
authors.lastname: Vemparala
authors.forename: William F
authors.initials: WF
authors.lastname: DeGrado
authors.forename: Michael L
authors.initials: ML
authors.lastname: Klein
mesh_terms: D003198:Computer Simulation; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000737:chemistry*; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism*; D017136:Ion Transport; D008958:Models, Molecular; D011487:Protein Conformation; D011522:Protons; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D007473:Ion Channels; D011522:Protons; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins