Improvement of amidase production by a newly isolated Delftia tsuruhatensis ZJB-05174 through optimization of culture medium.
The R-amidase production by a newly isolated strain of Delftia tsuruhatensis ZJB-05174 was optimized in this paper. Effects of factors such as carbon sources, nitrogen sources, and inducers on amidase production were investigated. The medium composition was optimized using central composite designs and response surface analysis. The optimal medium components for enhanced amidase production were found to be as follows: glucose, 8.23 g/l; yeast extract, 11.59 g/l; 2,2-(R,S)-dimethylcyclopropane carboxamide, 1.76 g/l; NaCl, 1 g/l; KH2PO4, 1 g/l; and K2HPO4, 1 g/l. A maximum enzyme production of 528.21 U/l was obtained under the optimized conditions, which was 4.7 times higher than that obtained under initial conditions.
Wang
Yuan-Shan
YS
Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, Zhejiang 310014, P. R. China.
Xu
Jian-Miao
JM
Zheng
Ren-Chao
RC
Zheng
Yu-Guo
YG
Shen
Yin-Chu
YC
D000581:Amidohydrolases / Q000096:biosynthesis*; D000704:Analysis of Variance; D001431:Bacteriological Techniques; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D042742:Delftia / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism; D012107:Research Design
D003470:Culture Media; D002244:Carbon; D000581:Amidohydrolases; C019665:amidase; D005947:Glucose; D009584:Nitrogen