title: MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells.
abstract: MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between normal brain and glioma tissues from Chinese patients by miRNA microarray and identified a panel of differentially expressed miRNAs. Here, we studied the function of one miRNA, miR-15b, in glioma carcinogenesis and elucidated its downstream targets. Over-expression of miR-15b resulted in cell cycle arrest at G0/G1 phase while suppression of miR-15b expression resulted in a decrease of cell populations in G0/G1 and a corresponding increase of cell populations in S phase. We further showed that CCNE1 (encoding cyclin E1) is one of the downstream targets of miR-15b. Taken together, our findings indicate that miR-15b regulates cell cycle progression in glioma cells by targeting cell cycle-related molecules.
authors.affiliation: Integrative Chemical Biology Laboratory, Institute of Molecular Technology, Department of Chemistry, 8/F, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Xia
authors.forename: Yanting
authors.initials: Y
authors.lastname: Qi
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Ng
authors.forename: Xiaona
authors.initials: X
authors.lastname: Chen
authors.forename: Shen
authors.initials: S
authors.lastname: Chen
authors.forename: Marong
authors.initials: M
authors.lastname: Fang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Ge
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Chen
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: He
authors.forename: Hsiang-fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Lihui
authors.initials: L
authors.lastname: Lai
authors.forename: Marie C
authors.initials: MC
authors.lastname: Lin
mesh_terms: D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D016213:Cyclins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: D016213:Cyclins; C505361:MIRN15 microRNA, human; D035683:MicroRNAs