title: [An enzyme electrode biosensor for inosine determination].
abstract: An enzyme electrode biosensor was used for the amperometric determination of inosine in its tablets by co-immobilizing nucleoside phosporylase and xanthine oxidase on a hydrogen peroxide electrode. As a fundamental electrode the hydrogen peroxide electrode has an advantage of stability in analysis compared with the 02 electrode. The enzyme electrode showed a linear response to inosine in the range of 1-268 mg/L with a response of 60 seconds under a sample injection volume of 25 microL. Based on the enzyme electrode, inosine solutions were determined with an average recover rate of 100.8% and a relative standard deviation (RSD) of les than 0.14% in 20 assays. The lifetime of the enzyme electrode was relative long and could be used continuously at 25 degrees C for 25 days. These results demonstrated that the enzyme electrode biosensor could be used to determine inosine and its derivatives specifically, rapidly, conveniently and economically.
authors.affiliation: Adrrssed Institute of Biology, Shandong Academy of Sciences, Shandong Key Laboratory for Biosensors, Ji'nan 250014, China sundaiman77@yahoo.com.cn
authors.forename: Shiqing
authors.initials: S
authors.lastname: Sun
authors.forename: Jianguo
authors.initials: J
authors.lastname: Shi
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.forename: Junhui
authors.initials: J
authors.lastname: Yang
authors.forename: Yaohong
authors.initials: Y
authors.lastname: Ma
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Meng
authors.forename: Dai
authors.initials: D
authors.lastname: Sun
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D007288:Inosine / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D007288:Inosine