Simultaneous in vivo dynamic magnetic resonance-diffuse optical tomography for small animal imaging.
We present simultaneous measurement of enhancement kinetics of an optical and a magnetic resonance (MR) contrast agent in a small animal breast tumor model (R3230 ac) using a combined MR-diffuse optical tomographic (MR-DOT) imaging system. A mixture of a small molecular-weight MR contrast agent gadolinium-diethylene-triamine-pentaacetic acid (Gd-DTPA) and a large molecular-weight optical contrast agent indocyanine green (ICG) was administered intravenously for multimodal dynamic imaging. Coregistration of optical and MR images was accomplished using agar-water-based markers. Using T(2) and dynamic T(1) weighted MR images, we divided the entire tumor into two regions of interest (ROI): a viable and a nonviable region. The absorption enhancements in the ROIs were calculated. An enhancement of the ICG was observed in the viable region. On the contrary, there was a lower enhancement in the nonviable region.
Unlu
Mehmet Burcin
MB
University of California, Tu and Yuen Center for Functional Onco-Imaging, Irvine, California 92697, USA. munlu@uci.edu
Lin
Yuting
Y
Birgul
Ozlem
O
Nalcioglu
Orhan
O
Gulsen
Gultekin
G
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology* / Q000662:veterinary; D003287:Contrast Media / Q000008:administration & dosage; D004338:Drug Combinations; D019786:Gadolinium DTPA / Q000008:administration & dosage; D007089:Image Enhancement / Q000379:methods*; D007208:Indocyanine Green / Q000008:administration & dosage; D008279:Magnetic Resonance Imaging / Q000379:methods / Q000662:veterinary*; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique / Q000662:veterinary*; D041622:Tomography, Optical / Q000379:methods / Q000662:veterinary*
D003287:Contrast Media; D004338:Drug Combinations; D007208:Indocyanine Green; D019786:Gadolinium DTPA