title: Prenyl alcohol production by expression of exogenous isopentenyl diphosphate isomerase and farnesyl diphosphate synthase genes in Escherichia coli.
abstract: Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 microg/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 microg/l).
authors.affiliation: Bio Research Laboratory, Toyota Motor Corporation, Toyota.
authors.forename: Chikara
authors.initials: C
authors.lastname: Ohto
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Shusei
authors.initials: S
authors.lastname: Obata
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakuradani
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D019748:Carbon-Carbon Double Bond Isomerases / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005204:Farnesol / Q000378:metabolism; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes
chemical_list: D004224:Diterpenes; D029968:Escherichia coli Proteins; D045782:Hemiterpenes; D005204:Farnesol; C017338:geranylgeraniol; D051228:Geranyltranstransferase; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase