Involvement of beta-lactamase inhibitory protein, BLIP-II, in morphological differentiation of Streptomyces exfoliatus SMF19.
The beta-lactamase inhibitory protein, BLIP-II, found in the culture supernatant of Streptomyces exfoliatus SMF19, shows no discernible sequence identity with other beta-lactamase inhibitory proteins identified in Streptomyces spp. A null mutant of the gene encoding BLIP-II (bliB::hygr) showed a bald appearance on solid media. Although BLIP-II was initially isolated from the supernatant of submerged cultures, sites of BLIP-II accumulation were seen in the cell envelope. The mutation of bliB was also associated with changes in the formation of septa and condensation of the chromosomal DNA associated with sporulation. The bliB mutant exhibited infrequent septa, showing dispersed chromosomal DNA throughout the mycelium, whereas the condensed chromosomes of the wild-type were separated by regularly spaced septa giving the appearance of a string of beads. Therefore, on the basis of these results, it is suggested that BLIP-II is a regulator of morphological differentiation in S. exfoliatus SMF19.
Kim
Eun Sook
ES
School of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
Song
Ju Yeon
JY
Kim
Dae Wi
DW
Ko
Eun Ji
EJ
Jensen
Susan E
SE
Lee
Kye Joon
KJ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009154:Mutation; D013171:Spores, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D065093:beta-Lactamase Inhibitors; C065596:beta-lactamase-inhibitor protein, Streptomyces; D001618:beta-Lactamases