Effect of human platelet-derived growth factor-BB on attachment of periodontal ligament cells on root surfaces.
OBJECTIVE
To evaluate the effects of platelet-derived growth factor-BB (PDGF-BB) on the attachment of human periodontal ligament cells (HPLCs) on the root surfaces demineralized with different agents.


METHODS
We performed this study at Ege University, Izmir, Turkey between 2005 and 2006. Eighty root slices were subjected to one of following treatments after root planing: 1) only root planing, 2) Platelet derived growth factor-BB (PDGF-BB), 3) citric acid demineralization, 4) citric acid demineralization + PDGF-BB, 5) tetracycline hydrochloric acid (T-HCl) demineralization, 6) T-HCl demineralization + PDGF-BB, 7) ethylenediamine tetra-acetic acid (EDTA) demineralization, and 8) EDTA demineralization + PDGF-BB. Human periodontal ligament cells were seeded on the root surfaces. Following the 2-hour incubation period, the number of cells was calculated by the colorimetric assay. Three slices from each group were processed for scanning electron microscopy. The number of attached cells was tested by analysis of variance (p=0.050).


RESULTS
There were no significant differences among the groups with regard to the mean number of attached cells (p=0.843), which was highest in the fourth group, and lowest in the sixth group.


CONCLUSIONS
Root planing is the most important treatment to make the diseased root surfaces biocompatible to HPLCs adherence. Application of PDGF-BB to root surfaces demineralized with citric acid may be advocated to enhance periodontal regeneration.
Becerik
Sema
S
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey. sema.cinar@ege.edu.tr
Sonmez
Sule
S
Sen
Bilge H
BH
Deliloglu-Gurhan
Ismet
I
Evrenosoglu
Evren
E
D000077214:Becaplermin; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D014092:Tooth Root / Q000166:cytology / Q000187:drug effects*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin