Distinct behaviors of adenylate kinase and cytochrome c observed following induction of mitochondrial permeability transition by Ca(2+) in the absence of respiratory substrate.
For induction of the mitochondrial permeability transition (PT) by Ca(2+), the addition of a respiratory substrate such as succinate is required. However, earlier studies indicated the possible induction of the mitochondrial PT by Ca(2+) in the absence of a respiratory substrate (Hunter, D.R., and Haworth, R.A. (1979) Arch. Biochem. Biophys. 195, 453-459). In the present study, we obtained clear evidence showing that the mitochondrial PT could be induced by Ca(2+) even in the absence of respiratory substrate. We next examined the protein release from mitochondria that accompanied the induction of PT in the absence of a respiratory substrate. Interestingly, distinct from the ordinary mitochondrial PT induced by Ca(2+) in the presence of a respiratory substrate, which is associated with the release of mitochondrial cytochome c and adenylate kinase, the mitochondrial PT occurring in the absence of a respiratory substrate was associated with release of mitochondrial adenylate kinase but not with that of mitochondrial cytochrome c. This experimental system should be quite useful for understanding the mechanisms of protein release from mitochondria.
Yamamoto
Takenori
T
Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima, 770-8503, Japan.
Yoshimura
Yuya
Y
Yamada
Akiko
A
Gouda
Shunichi
S
Yamashita
Kikuji
K
Yamazaki
Naoshi
N
Kataoka
Masatoshi
M
Nagata
Toshihiko
T
Terada
Hiroshi
H
Shinohara
Yasuo
Y
D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000008:administration & dosage*; D019069:Cell Respiration / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects / Q000502:physiology*; D000083162:Mitochondrial Permeability Transition Pore; D051381:Rats; D017208:Rats, Wistar
D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D045304:Cytochromes c; D000263:Adenylate Kinase; D002118:Calcium