Effects of hyperbaric oxygenation on cultured barley embryos.
Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vulgare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, 37.5%, and 94.98% for the 10th days of culture, respectively, than in the control plants. Increase in SOD activity was also shown on polyacrilamide gel electrophoresis on the 10th day of application. Proline accumulation was increased in HBO-treated explants both on the 5th days (85.71%) and 10th days (37.14%) of treatment. MDA content was found to be higher in HBO treated explants both on the 5th (53.84%) and 10th (59.83%) days of culture.
Arican
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E
Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134, Vezneciler-Istanbul, Turkey. earican@istanbul.edu.tr
Gozukirmizi
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N
D000975:Antioxidants / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D001467:Hordeum / Q000196:embryology / Q000378:metabolism*; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation; D008954:Models, Biological; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D011312:Pressure; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D046509:Tissue Culture Techniques; D014867:Water / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D014867:Water; D011392:Proline; D009195:Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase