Proteomic analysis of the Spodoptera frugiperda ascovirus 1a virion reveals 21 proteins.
The Spodoptera frugiperda ascovirus 1a (SfAV-1a) is a double-stranded DNA virus that attacks lepidopteran larvae, in which it produces enveloped virions with complex symmetry which have an average diameter of 130 nm and length of 400 nm. Here, we report identification of 21 SfAV-1a-encoded proteins that occur in the virion, as determined by nano-liquid chromatography/tandem mass spectrometry. These included a helicase (ORF009), nuclease (ORF075), ATPase (ORF047), serine/threonine-like protein kinase (ORF064), inhibitor of apoptosis-like protein (ORF015), thiol oxidoreductase-like protein (ORF061), CTD phosphatase (ORF109), major capsid protein (ORF041) and a highly basic protein, P64 (ORF048). The latter two were the most abundant. Apart from ascoviruses, the closest orthologues were found in iridoviruses, providing further evidence that ascoviruses evolved from invertebrate iridoviruses. These results establish a foundation for investigating how ascovirus virion proteins interact to form their complex asymmetrical structure, as well as for elucidating the mechanisms involved in SfAV-1a virion morphogenesis.
Tan
Yeping
Y
Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA.
Bideshi
Dennis K
DK
California Baptist University, Department of Natural and Mathematical Sciences, 8432 Magnolia Avenue Riverside, CA 92504, USA.|Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA.
Johnson
Jeffrey J
JJ
Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA.
Bigot
Yves
Y
CHRU de Tours, Université Francois Rabelais, UFR des Sciences & Techniques, Parc de Grandmont, 37200 Tours, France.|CNRS, UMR 6239, Génétique, Immunothérapie, Chimie et Cancer, Université Francois Rabelais, UFR des Sciences & Techniques, Parc de Grandmont, 37200 Tours, France.
Federici
Brian A
BA
Interdepartmental Graduate Programs in Genetics and Cell, Molecular & Developmental Biology, University of California Riverside, Riverside, CA 92521, USA.|Department of Entomology, University of California, Riverside, Riverside, CA 92521, USA.
D000595:Amino Acid Sequence; D000818:Animals; D029223:Ascoviridae / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002853:Chromatography, Liquid; D017124:Conserved Sequence; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020543:Proteome; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D014771:Virion / Q000235:genetics
D036022:Capsid Proteins; D020543:Proteome; D014764:Viral Proteins