Mutagenesis of the nucleocapsid protein of Nipah virus involved in capsid assembly.
The nucleocapsid protein of Nipah virus produced in Escherichia coli assembled into herringbone-like particles. The amino- and carboxy-termini of the N protein were shortened progressively to define the minimum contiguous sequence involved in capsid assembly. The first 29 aa residues of the N protein are dispensable for capsid formation. The 128 carboxy-terminal residues do not play a role in the assembly of the herringbone-like particles. A region with amino acid residues 30-32 plays a crucial role in the formation of the capsid particle. Deletion of any of the four conserved hydrophobic regions in the N protein impaired capsid formation. Replacement of the central conserved regions with the respective sequences from the Newcastle disease virus restored capsid formation.
Ong
Swee Tin
ST
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Yusoff
Khatijah
K
Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.|Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Kho
Chiew Ling
CL
Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, UTAR Complex, Jalan Genting Kelang, 53300 Setapak, Kuala Lumpur, Malaysia.
Abdullah
Janna Ong
JO
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Tan
Wen Siang
WS
Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.|Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
D000595:Amino Acid Sequence; D002213:Capsid / Q000502:physiology*; D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers; D004926:Escherichia coli / Q000821:virology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D045405:Nipah Virus / Q000235:genetics* / Q000648:ultrastructure; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000648:ultrastructure; D017384:Sequence Deletion
D036022:Capsid Proteins; D017931:DNA Primers; D019590:Nucleocapsid Proteins