title: Effects of two new steroids and cyproterone on some biomarkers of oxidative stress and serotonergic system on rat prostate and brain.
abstract: The purpose of this study was to evaluate the effect of cyproterone acetate (CPA, A) compared with new synthetic steroids 3alpha-acetoxy-5,6-epoxy-16-pregnen-20-one (B) and 17alpha-hydroxy-16beta-methyl-1,4,6-pregnatriene-3,20-dione (C) in rat prostate and brain. Groups of animals were treated either with A, B or C (4 mg kg(-1) day(-1)) by the intraperitoneal route for 5 days. Levels of reduced glutathione (GSH), 5-hydroxy-indole acetic acid (5-HIAA), lipid peroxidation (as thiobarbituric acid reactive substances, TBARS) and the activities of Na(+), K(+)- and total ATPases were assayed in prostate and brain for each group of animals including a control group. No appreciable changes were shown in Na(+), K(+)-ATPase and total ATPases and TBARS on prostate and brain of rats that received A, B and C steroids. However, the levels of GSH and 5-HIAA decreased significantly (P < 0.05) in both tissues for the steroids assayed. It is concluded that CPA and the homologues B and C steroids induce changes in the levels of GSH and serotonin in rat prostate and brain.
authors.affiliation: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Mexico City, Mexico.
authors.forename: G D
authors.initials: GD
authors.lastname: Calderón
authors.forename: E
authors.initials: E
authors.lastname: Bratoeff
authors.forename: L E
authors.initials: LE
authors.lastname: Ramírez
authors.forename: B N
authors.initials: BN
authors.lastname: Osnaya
authors.forename: A R
authors.initials: AR
authors.lastname: García
authors.forename: M G
authors.initials: MG
authors.lastname: Barragán
authors.forename: G-E
authors.initials: GE
authors.lastname: Hernández
authors.forename: O H
authors.initials: OH
authors.lastname: Juárez
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D017373:Cyproterone Acetate / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011281:Pregnatrienes / Q000494:pharmacology*; D011284:Pregnenolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: C539190:17-hydroxy-16-methyl-1,4,6-pregnatriene-3,20-dione; C539189:3-acetoxy-5,6-epoxy-16-pregnen-20-one; D015415:Biomarkers; D011281:Pregnatrienes; D017392:Thiobarbituric Acid Reactive Substances; D012701:Serotonin; D017373:Cyproterone Acetate; D006897:Hydroxyindoleacetic Acid; D011284:Pregnenolone; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione