Development of analytical methods for multiplex bio-assay with inductively coupled plasma mass spectrometry.
Advances in the development of highly multiplexed bio-analytical assays with inductively coupled plasma mass spectrometry (ICP-MS) detection are discussed. Use of novel reagents specifically designed for immunological methods utilizing elemental analysis is presented. The major steps of method development, including selection of elements for tags, validation of tagged reagents, and examples of multiplexed assays, are considered in detail. The paper further describes experimental protocols for elemental tagging of antibodies, immunostaining of live and fixed human leukemia cells, and preparation of samples for ICP-MS analysis. Quantitative analysis of surface antigens on model cell lines using a cocktail of seven lanthanide labeled antibodies demonstrated high specificity and concordance with conventional immunophenotyping.
Ornatsky
Olga I
OI
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Room 407, 164 College Street, Toronto, Ontario, Canada, M5S 3G9.
Kinach
Robert
R
Bandura
Dmitry R
DR
Lou
Xudong
X
Tanner
Scott D
SD
Baranov
Vladimir I
VI
Nitz
Mark
M
Winnik
Mitchell A
MA