Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.
Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.
Matsuno
Akira
A
Department of Neurosurgery, Teikyo University Chiba Medical Center, Anesaki, Ichihara City, Chiba, Japan. akirakun@med.teikyo-u.ac.jp
Itoh
Johbu
J
Mizutani
Akiko
A
Takekoshi
Susumu
S
Osamura
R Yoshiyuki
RY
Okinaga
Hiroko
H
Ide
Fuyuaki
F
Miyawaki
Satoru
S
Uno
Takeshi
T
Asano
Shuichiro
S
Tanaka
Junichi
J
Nakaguchi
Hiroshi
H
Sasaki
Mitsuyoshi
M
Murakami
Mineko
M
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002457:Cell Extracts; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D013006:Growth Hormone / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014162:Transfection; D020692:rab3 GTP-Binding Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D002457:Cell Extracts; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C527701:enhanced cyan fluorescent protein; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D013006:Growth Hormone; C492132:Rab3b protein, rat; D020692:rab3 GTP-Binding Proteins