Therapeutic advantages of medicinal herbs fermented with Lactobacillus plantarum, in topical application and its activities on atopic dermatitis.
The use of herbal medicines in the therapeutic treatment of atopic dermatitis (AD) has been suggested recently. The present study examined whether selected herbal extracts fermented in Lactobacillus plantarum (FHE) possessed anti-AD properties. In addition, the study assessed the increased bioavailability of these herbal extracts both in vitro and in vivo. The data from these experiments revealed that FHE inhibited the proliferation of splenic T and B cells in a dose-dependent manner, when activated with their mitogens. Moreover, the expression of Th1/Th2 mRNA cytokines (IL-2, IL-4, IL-5, IL-13) from mouse splenocytes was inhibited severely as was cyclosporine A. Furthermore, the release of beta-hexosaminidase in RBL-2H3 mast cells was suppressed significantly. FHE also reduced the plasma level of IgE in dust mite extract-induced AD-like NC/Nga mice. More dramatic results were found in the histological changes, which were observed by hematoxylin-eosin and toluidine blue staining, as well as in the macroscopic features on dorsal lesions of AD-like NC/Nga mice. In conclusion, the results presented in this study suggest that FHE may have therapeutic advantages for the treatment of AD due to its increased immune-suppressive and increased absorptive effects, which were fortified by L. plantarum fermentation.
Joo
Seong Soo
SS
Research Institute of Veterinary Medicine, Chungbuk National University, 361-763, Republic of Korea.
Won
Tae Joon
TJ
Nam
Sang Yoon
SY
Kim
Yun-Bae
YB
Lee
Young Chul
YC
Park
So-Yong
SY
Park
Hee Yong
HY
Hwang
Kwang Woo
KW
Lee
Do Ik
DI
D000287:Administration, Topical; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D004195:Disease Models, Animal; D005285:Fermentation; D029001:Herbal Medicine; D007073:Immunoglobulin E / Q000097:blood; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012867:Skin / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects / Q000378:metabolism
D016207:Cytokines; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D007073:Immunoglobulin E