title: Biochemical responses and accumulation properties of long-chain perfluorinated compounds (PFOS/PFDA/PFOA) in juvenile chickens (Gallus gallus).
abstract: One-day-old male chickens were exposed via oral gavage to mixtures of perfluorooctane sulfonate (PFOS), perfluorooctanoate (PFOA), and perfluorodecanoate (PFDA) at either a low dose (0.1 mg/kg body weight [b.w.]) or a high dose (1.0 mg/kg b.w.), or a saline/ethanol vehicle control, three times a week for 3 weeks. After 3 weeks of exposure, half of the chicks were sacrificed and the other half were allowed to depurate for a further 3 weeks. No dose-dependent statistically significant differences in body/organ weights were observed among treatment and control groups after 3 weeks of exposure or after three 3 of depuration. Neither 15 histological nor 14 measured plasma biochemical parameters were significantly different in chicks from the exposed groups and vehicle controls. PFOS, PFDA, and PFOA concentrations in blood/liver/kidney samples were measured throughout the exposure and depuration periods at different time intervals. PFOS and PFDA accumulated at much higher concentrations than PFOA during the experimental periods. Interestingly, PFOS and PFDA accumulation patterns in the blood were similar during the exposure and depuration periods. The half-lives for each PFC at the 0.1 and 1.0 mg/kg doses were, respectively, approximately 15 and 17 days for PFOS, 11 and 16 days for PFDA, and 3.9 and 3.9 days for PFOA. PFDA accumulation in organs was greater than or similar to that of PFOS: the liver was the main target during exposure and the blood was the main reservoir during depuration. These results indicate that exposure to a 1.0-mg mixture of PFOS/PFDA/PFOA/kg b.w. has no adverse effect on juvenile chickens.
authors.affiliation: Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Leo W Y
authors.initials: LW
authors.lastname: Yeung
authors.forename: Eva I H
authors.initials: EI
authors.lastname: Loi
authors.forename: Vicky Y Y
authors.initials: VY
authors.lastname: Wong
authors.forename: Keerthi S
authors.initials: KS
authors.lastname: Guruge
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamanaka
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Tanimura
authors.forename: Jun
authors.initials: J
authors.lastname: Hasegawa
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Yamashita
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Lam
mesh_terms: D000818:Animals; D002138:Calibration; D002645:Chickens / Q000378:metabolism* / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005466:Fluorocarbons / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity*; D006207:Half-Life; D007202:Indicators and Reagents; D008297:Male; D013058:Mass Spectrometry; D011786:Quality Control; D014018:Tissue Distribution
chemical_list: D005466:Fluorocarbons; D007202:Indicators and Reagents