title: Reconstructing mandibular defects using autologous tissue-engineered tooth and bone constructs.
abstract: OBJECTIVE
  Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model.
  
  
  METHODS
  Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses.
  
  
  RESULTS
  Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues.
  
  
  CONCLUSIONS
  This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, USA.
authors.forename: Harutsugi
authors.initials: H
authors.lastname: Abukawa
authors.forename: Weibo
authors.initials: W
authors.lastname: Zhang
authors.forename: Conan S
authors.initials: CS
authors.lastname: Young
authors.forename: Rose
authors.initials: R
authors.lastname: Asrican
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Vacanti
authors.forename: Leonard B
authors.initials: LB
authors.lastname: Kaban
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Troulis
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Yelick
mesh_terms: D053523:Amelogenin / Q000096:biosynthesis; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000637:transplantation; D005240:Feasibility Studies; D008297:Male; D008334:Mandible / Q000166:cytology / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D023421:Models, Animal; D009805:Odontogenesis / Q000502:physiology*; D010006:Osteoblasts / Q000637:transplantation*; D010750:Phosphoproteins / Q000096:biosynthesis; D010865:Pilot Projects; D012038:Regeneration / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014070:Tooth / Q000166:cytology / Q000378:metabolism; D014083:Tooth Germ / Q000637:transplantation*
chemical_list: D053523:Amelogenin; D010750:Phosphoproteins