Carbonic anhydrase inhibitors: inhibition studies of a coral secretory isoform with inorganic anions.
The inhibition of a coral carbonic anhydrase (CA, EC 4.2.1.1) has been investigated with a series of inorganic anions such as halogenides, pseudohalogenides, bicarbonate, carbonate, nitrate, nitrite, hydrogen sulfide, bisulfite, perchlorate, sulfate. The full-length scleractinian coral Stylophora pistillata CA, STPCA, has a significant catalytic activity for the physiological reaction of CO(2) hydration to bicarbonate, similarly to the ubiquitous human isoforms hCA I (cytosolic) and hCA VI (secreted). The best STPCA anion inhibitors were bromide, iodide, carbonate, and sulfamate, with inhibition constants of 9.0-10.0 microM.
Bertucci
Anthony
A
Centre Scientifique de Monaco, Avenue Saint-Martin, MC-98000 Principality of Monaco, Monaco.
Innocenti
Alessio
A
Zoccola
Didier
D
Scozzafava
Andrea
A
Allemand
Denis
D
Tambutté
Sylvie
S
Supuran
Claudiu T
CT
D000086:Acetazolamide / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D000838:Anions / Q000737:chemistry; D037421:Anthozoa; D001665:Binding Sites; D002245:Carbon Dioxide / Q000737:chemistry; D002257:Carbonic Anhydrase Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry*; D002256:Carbonic Anhydrases / Q000737:chemistry*; D015195:Drug Design; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D017386:Sequence Homology, Amino Acid
D000838:Anions; D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D020033:Protein Isoforms; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D000086:Acetazolamide