title: Sero-characterization of lipopolysaccharide from Burkholderia thailandensis.
abstract: We report the successful purification of lipopolysaccharide (LPS) from Burkholderia thailandensis, a Gram-negative bacterium, closely related to the highly pathogenic organisms B. pseudomallei and B. mallei. Burkholderia thailandensis LPS is shown to cross-react with rabbit and mouse sera obtained from inoculation with B. pseudomallei or B. mallei, respectively. These data suggest that B. thailandensis LPS shares similar structural features with LPS molecules from highly pathogenic Burkholderia species. This information may prove useful in ongoing efforts to develop novel vaccines and/or diagnostic reagents.
authors.affiliation: Department of Pediatrics and the Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Qazi
authors.forename: Joann L
authors.initials: JL
authors.lastname: Prior
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Judy
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Whitlock
authors.forename: G Barrie
authors.initials: GB
authors.lastname: Kitto
authors.forename: Alfredo G
authors.initials: AG
authors.lastname: Torres
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Brown
mesh_terms: D000818:Animals; D019117:Burkholderia / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D012329:RNA, Bacterial / Q000302:isolation & purification; D011817:Rabbits; D013045:Species Specificity
chemical_list: D008070:Lipopolysaccharides; D012329:RNA, Bacterial