title: Isolation and physiological characterization of Bacillus clausii SKAL-16 isolated from wastewater.
abstract: An alkaliphilic bacterium, Bacillus clausii SKAL-16, was isolated from soil that had been contaminated with vegetable oil. The optimal pH and general pH range for bacterial growth was 8, and 7 to 10, respectively. The bacterium could grow on tributyrin and glycerol, but could not grow on acetate and butyrate. The SKAL-16 strain excreted butyric acid during growth on tributyrin, and selectively ingested glycerol during growth on a mixture of butyric acid and glycerol. The SKAL-16 generated intracellular lipase, but did not produce esterase and extracellular lipase. The DNA fragment amplified with the chromosomal DNA of SKAL-16 and primers designed on the basis of the esterase-coding gene of Bacillus clausii KSM-K16 was not identical with the esterase-coding gene contained in the GenBank database. Pyruvate dehydrogenase, isocitrate dehydrogenase, and malate dehydrogenase activities were detected in the cellfree extract (crude enzyme).
authors.affiliation: Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea.
authors.forename: Sung Hun
authors.initials: SH
authors.lastname: Lee
authors.forename: Doo Hyun
authors.initials: DH
authors.lastname: Park
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001483:Base Sequence; D002087:Butyrates / Q000378:metabolism; D002952:Citric Acid Cycle / Q000502:physiology; D004950:Esterases / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism; D008969:Molecular Sequence Data; D010938:Plant Oils; D015203:Reproducibility of Results; D012988:Soil Microbiology; D014280:Triglycerides / Q000378:metabolism; D014866:Waste Products
chemical_list: D002087:Butyrates; D010938:Plant Oils; D014280:Triglycerides; D014866:Waste Products; D004950:Esterases; D008049:Lipase; D005990:Glycerol; C005830:tributyrin