Characterization of Capsicum annuum recombinant alpha- and beta-tubulin.
There are several conditions which might modulate polymerization to produce polymers having normal lattice structure. In the absence of 1 mM MgCl(2) the assembly was reduced by 36% in Capsicum annuum tubulin (CAnm tubulin). There was no significant difference in the final assembly formation in the presence of 5% to 10% glycerol. However, nucleation rate was slow and apparent study state was achieved lately in the presence of 10% glycerol. Taxol at 100 microM concentration increased 23% tubulin assembly. One millimolar CaCl(2), >or=1% dimethyl sulfoxide (DMSO) and physiologically low temperature reduced CAnm tubulin assembly. A value of 0.089 mg/ml was obtained as critical concentration for polymerization. Benomyl significantly reduced the number of cysteine residues accessible to 5,5'-dithiobis-(2-nitrobenzoic acid); there were 4.77 +/- 0.21 and 3.49 +/- 0.35 residues accessible per tubulin dimer in the presence of 50 and 100 microM benomyl respectively.
Koo
Bon-Sung
BS
Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon, Korea.
Jang
Myung-Hyun
MH
Park
Haechul
H
Kalme
Satish
S
Park
Hey-Yeon
HY
Han
Jin-Wook
JW
Yeo
Yun-Soo
YS
Yoon
Sang-Hong
SH
Kim
Soo-Jin
SJ
Lee
Chang-Muk
CM
Yoon
Moon-Young
MY
D002212:Capsicum; D004228:Dithionitrobenzoic Acid / Q000378:metabolism; D007202:Indicators and Reagents / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D055503:Protein Multimerization / Q000187:drug effects; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*
D007202:Indicators and Reagents; D010940:Plant Proteins; D011994:Recombinant Proteins; D014404:Tubulin; D004228:Dithionitrobenzoic Acid