Localization and role of galanin in the thyroid gland of Podarcis sicula lizard (reptilia, lacertide).
Galanin (GAL) is a 29-amino acid residue neuropeptide, which was initially isolated from porcine intestine extracts and since then, widely found in a variety of vertebrate organs, in correlation with multiple neuro-hormonal actions exerted and so receiving a constantly growing attention. Moreover, although the studies undertaken so far suggest a local intrathyroidal peptidergic regulatory action, the exact role of GAL on thyroid gland remains to be established. The aim of this study was to determine in the lizard, Podarcis sicula, (1) the presence of GAL immunoreactivity in the thyroid gland and (2) the short- and long-term effects of in vivo GAL administration by intraperitoneal injection on thyroid gland physiology. First of all, the presence of GAL in the thyroid gland of P. sicula was demonstrated by immunohistochemical technique (avidin-biotin-peroxidase complex--ABC method). Second, the role of GAL in the control of thyroid gland activity was studied in vivo using light microscopy (LM) technique coupled to a specific radioimmunoassay for thyroid-stimulating hormone (TSH) and thyroid hormones (T(4) and T(3)). Prolonged GAL administration [(0.4 mg/100 g body wt)/day] increased T(4) and T(3) release, but decreased the plasma concentration of TSH. In addition, using LM clear signs of stimulation of the thyroid gland were observed. These findings suggest that systemic administration of GAL was able to stimulate the thyroid gland of the lizard both at morphological and physiological level.
Sciarrillo
Rosaria
R
Department of Biological and Environmental Sciences, University of Sannio, Benevento, Italy. sciarrillo@unisannio.it
Capaldo
Anna
A
Valiante
Salvatore
S
Laforgia
Vincenza
V
De Falco
Maria
M
D000818:Animals; D019004:Galanin / Q000378:metabolism* / Q000494:pharmacology*; D007150:Immunohistochemistry; D008116:Lizards / Q000097:blood / Q000378:metabolism*; D008297:Male; D012621:Seasons; D013961:Thyroid Gland / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D014284:Triiodothyronine; D019004:Galanin; D013972:Thyrotropin; D013974:Thyroxine