title: Long-term EGF treatment partially prevents reduction of renal blood flow in response to neonatally induced partial unilateral ureteral obstruction.
abstract: OBJECTIVE
  To investigate the effects of long-term epidermal growth factor (EGF) treatment on kidney growth, renal blood flow (RBF), AQP2 expression and urine output in newborn rats with partial unilateral ureteral obstruction (PUUO).
  
  
  METHODS
  PUUO (n = 29) and sham-operated control (CON) (n = 26) was created in 2-day-old rats. Fifteen PUUO and 14 CON rats were treated with EGF beginning from the second day after operation to 8 weeks of age. Total kidney volume (TKV) and RBF were measured by MRI at weeks 4 and 8, respectively. Urine volume and osmolality, plasma osmolality, and free water clearance were measured. Renal AQP2 expression was determined using semiquantitative immuno-blotting.
  
  
  RESULTS
  PUUO induced a severe hydronephrosis and RBF reduction in the obstructed kidney which was attenuated in EGF-treated rats. Body weight, urine output and free water clearance increased, osmolality and the osmolar clearance decreased significantly in EGF-treated rats, despite unchanged AQP2 levels.
  
  
  CONCLUSIONS
  Long-term EGF treatment attenuated the obstruction- induced RBF reduction. Body weight, urine output increased, and urine osmolality decreased in both control and PUUO rats treated with EGF. Solute-free water clearance increased in PUUO rats which seem to be independent of AQP2 expression.
authors.affiliation: Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark.
authors.forename: Jian Guo
authors.initials: JG
authors.lastname: Wen
authors.forename: Gui Xian
authors.initials: GX
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Steffen
authors.initials: S
authors.lastname: Ringgaard
authors.forename: Lars
authors.initials: L
authors.lastname: Vinter-Jensen
authors.forename: Troels Munch
authors.initials: TM
authors.lastname: Jørgensen
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Li
authors.forename: Hans
authors.initials: H
authors.lastname: Stødkilde-Jørgensen
authors.forename: Søren
authors.initials: S
authors.lastname: Nielsen
authors.forename: Jens Christian
authors.initials: JC
authors.lastname: Djurhuus
authors.forename: Jørgen
authors.initials: J
authors.lastname: Frøkiaer
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014517:Ureteral Obstruction / Q000188:drug therapy* / Q000503:physiopathology / Q000652:urine
chemical_list: D004815:Epidermal Growth Factor