Neuronal release of proBDNF.
Pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF utilize distinct receptors to mediate divergent neuronal actions. Using new tools to quantitate endogenous BDNF isoforms, we found that mouse neurons secrete both proBDNF and mature BDNF. The highest levels of proBDNF and p75 were observed perinatally and declined, but were still detectable, in adulthood. Thus, BDNF actions are developmentally regulated by secretion of proBDNF or mature BDNF and by local expression of p75 and TrkB.
Yang
Jianmin
J
Department of Medicine, Weill Cornell Medical College, 1300 York Ave., New York, New York 10065, USA.
Siao
Chia-Jen
CJ
Nagappan
Guhan
G
Marinic
Tina
T
Jing
Deqiang
D
McGrath
Kelly
K
Chen
Zhe-Yu
ZY
Mark
Willie
W
Tessarollo
Lino
L
Lee
Francis S
FS
Lu
Bai
B
Hempstead
Barbara L
BL
D000818:Animals; D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D055879:Gene Knock-In Techniques; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D008817:Mice, Mutant Strains; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism
D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor; D011498:Protein Precursors; D020800:Receptor, Nerve Growth Factor; C426674:brain-derived neurotrophic factor precursor; D020813:Receptor, trkB