title: Effect of NO-generating compound NaNO2 on ultrastructure of synaptic vesicles of glutamatergic synapses.
abstract: Ultrastructure of synaptic vesicles in axon terminals of granule cells from isolated cerebellum of Rana temporaria frogs under the influence of NO-generating compound NaNO2 in various concentrations and electrical stimulation was evaluated by the method of electron microscopy. NO-generating compound in low concentration induced translocation of synaptic vesicles and formation of small clusters. The size and structure of synaptic vesicles remained unchanged under these conditions. Increasing the concentration of NaNO2 led to swelling of synaptic vesicles, formation of arranged heaps from individual vesicles or fusion of their content. Electrical stimulation of the cerebellum in the presence of NaNO2 increased damage to synaptic vesicles. These experimental data model some stages observed in stroke. The formation of clusters from synaptic vesicles is a compensatory and adaptive response maintaining the structure of synaptic vesicles and protecting neurons from high concentrations of glutamate. Glutamate produces a toxic effect on nerve cells and glial cells of the cerebellum under pathological conditions, which is accompanied by impairment of signal transduction from presynaptic to postsynaptic neurons.
authors.affiliation: Laboratory No. 12, A. A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Samosudova
authors.forename: V P
authors.initials: VP
authors.lastname: Reutov
authors.forename: N P
authors.initials: NP
authors.lastname: Larionova
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology; D018698:Glutamic Acid / Q000378:metabolism*; D007202:Indicators and Reagents / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism*; D011896:Rana temporaria / Q000033:anatomy & histology; D012977:Sodium Nitrite / Q000494:pharmacology*; D013569:Synapses / Q000187:drug effects / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D007202:Indicators and Reagents; D009569:Nitric Oxide; D018698:Glutamic Acid; D012977:Sodium Nitrite