[Melatonin promotes and synchronizes protein synthesis in hepatocyte culture from old rats].
The effect of 1 to 1000 nM melatonin was studied on daily cultures of rat hepatocytes on slides in serum-free medium. The minimum melatonin concentration (1 nM) proved to synchronize protein synthesis in asynchronous sparse cultures of hepatocytes from rats of different age, and a circahoralian rhythm of protein synthesis was revealed in them. In dense weekly synchronous hepatocytes from old rats (2.5-years-old with the weight of about 600 g), melatonin improved cell synchronization to the level of young animals. Melatonin treatment increased the mean rate of protein synthesis in rats of different age.
Brodskiĭ
V Ia
VIa
Golichenkov
V A
VA
Zvezdina
N D
ND
Dubovaia
T K
TK
Fateeva
V I
VI
Mal'chenko
L A
LA
Burlakova
O V
OV
Bespiatykh
A Iu
AIu
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D008550:Melatonin