Breeding of industrial diploid yeast strain with chromosomal integration of multiple beta-glucosidase genes.
We constructed a double auxotrophic OC-2 industrial diploid strain of Saccharomyces cerevisiae and introduced 4 copies of cell surface displaying beta-glucosidase (BGL) genes into the chromosome. The engineered OC-2 strain showed 5-fold higher BGL activity compared with the yeast carrying 2 copies of BGL gene and directly produced ethanol from cellobiose.
Saitoh
Satoshi
S
Toyota Biotechnology & Afforestation Buisiness Div, Toyota Motor Co., 1099 Aza Marune, Oaza Kurozasa, Miyoshi-cho, Nishikamogun, Aichi 470-0201, Japan. satoshi_saito_ab@mail.toyota.co.jp
Tanaka
Tsutomu
T
Kondo
Akihiko
A
D001483:Base Sequence; D015825:Chromosomes, Fungal; D017931:DNA Primers; D004171:Diploidy; D005285:Fermentation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D001617:beta-Glucosidase / Q000235:genetics*
D017931:DNA Primers; D001617:beta-Glucosidase