Reporter gene assays in Candida albicans.
Reporter systems are used in Candida albicans in three major experimental areas. These include gene expression, promoter analysis, and protein expression/localization. Heterologous expression in C. albicans is either not effective or inefficient due to the alternative codon usage in Candida, particularly CTG. Consequently, several reporter genes have been constructed by optimizing codons for expression in Candida. The reporter systems include lacZ, luciferase, and GFP. Generally, PCR site directed mutagenesis has been used to construct the modified reporter. Reporter gene vectors are not commercially available for Candida, but they can normally be requested from the laboratories that developed the constructs.
Sturtevant
Joy
J
Department of Microbiology, Immunology and Parasitology, Louisiana State University School of Medicine, New Orleans, LA, USA.
D002176:Candida albicans / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D049452:Green Fluorescent Proteins; D008156:Luciferases; D001616:beta-Galactosidase