title: Retroviral gene transduction into chicken embryo gonads through blood circulation.
abstract: A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Naka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Komatsu
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nishijima
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D000818:Animals; D001483:Base Sequence; D001775:Blood Circulation; D002642:Chick Embryo; D017931:DNA Primers; D005822:Genetic Vectors; D005854:Germ Cells / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D017931:DNA Primers