[Role of genistein in enzymatic albumin hydrolysis in the presence of nitrates (III) and (V)].
Polyphenols and nitrates are essential ingredients of human diet. Harm caused by nitrates is well know and studied. Positive role of polyphenols is investigated. The aim of the study was to analyze interactions between nitrates (III) and (V) and genistein in systems of enzymatic protein (albumin) hydrolysis. In vitro model of enzymatic acidic-alkaline albumine hydrolysis in the presence of nitrates, polyphenols and vitamin C in different concentrations was used. Content of nitrates was measured in dialysation fluid spectrophotometrically according to Griess' method. The study revealed inhibiting influence of genistein on nitrares(III) concentration in external compartment. The influence depended on polyphenol dose (for nitrates (III) between 11.21% and 7.27%, for nitrates (V) between 95.64% and 79.64% of dialysis). When genistein was introduced in too high concentrations--over 2,4 mg/system--it did not improve the effect, but inhibited it. The influence of genistein was synergic with resveratrol and vitamin C.
Tokarz
Andrzej
A
Katedra i Zakład Bromatologii, Warszawski Uniwersytet Medyczny, 02-097 Warszawa, ul. Banacha 1. bromatos@wum.edu.pl
Pokorska-Lis
Grazyna
G
Popiel
Elzbieta
E
D000418:Albumins / Q000737:chemistry*; D015314:Dialysis Solutions / Q000737:chemistry*; D004357:Drug Synergism; D005419:Flavonoids / Q000737:chemistry*; D019833:Genistein / Q000737:chemistry*; D006868:Hydrolysis; D009566:Nitrates / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols
D000418:Albumins; D015314:Dialysis Solutions; D005419:Flavonoids; D009566:Nitrates; D010636:Phenols; D059808:Polyphenols; D019833:Genistein