Piecing together the ESCRTs.
High-resolution structural analysis has characterized nearly all of the individual domains of ESCRT (endosomal sorting complex required for transport) subunits, all of the core structures of the soluble complexes and many of the interactions involving domains. Recent emphasis in structural studies has shifted towards efforts to integrate these structures into a larger-scale model. Molecular simulations, hydrodynamic analysis, small-angle X-ray scattering and cryo-EM (electron microscopy) techniques have all been brought to bear on the ESCRT system over the last year.
Hurley
James H
JH
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USA. hurley@helix.nih.gov
Im
Young Jun
YJ
Lee
Hyung Ho
HH
Ren
Xuefeng
X
Wollert
Thomas
T
Yang
Dong
D
D011992:Endosomes / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000378:metabolism*; D021381:Protein Transport; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000378:metabolism
D046912:Multiprotein Complexes; D033921:Vesicular Transport Proteins