[NF-kappaB modulates activation of the BMP-2 gene by trichostatin A].
In this study, we investigated trichostatin A (TSA), a histone deacetylase inhibitor, increased the Bone morphogenetic protein-2 (BMP-2) mRNA level in human osteoblasts line. Deletion analysis of the promoter region revealed that TSA-induced luciferase was regulated by the BMP-2 promoter spanning from -320 to-310. Electrophoresis mobility shift assay (EMSA) and Chromatin immunoprecipitation (CHIP) assay proved that this position was nuclear factor NF-kappaB responsive element. The results above demonstrated that acetylation plays a crucial role in BMP-2 expression and acetylation of NF-kappaB p65 and p50 subunits by TSA treatment may activate the BMP-2 promoter.
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D000107:Acetylation / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020218:Response Elements / Q000502:physiology*; D017384:Sequence Deletion; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D052002:NF-kappa B p50 Subunit; D012333:RNA, Messenger; D051996:Transcription Factor RelA; C012589:trichostatin A