Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector.
A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.
Kaneko
Shinya
S
Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, Japan.
Takeuchi
Takashi
T
Itaya
Mitsuhiro
M
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005822:Genetic Vectors; D016680:Genome, Bacterial; D015698:Genomic Library; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D063151:Polycomb Repressive Complex 2; D011995:Recombination, Genetic; D014170:Transformation, Genetic
C093650:Jarid2 protein, mouse; D009419:Nerve Tissue Proteins; D063151:Polycomb Repressive Complex 2