Calorimetric studies of the effect of cis-carotenoids on the thermotropic phase behavior of phosphatidylcholine bilayers.
Carotenoid geometry is a factor that determines their solubility and orientation in the lipid membrane as well as antioxidant capacities and bioavailability. The effects of the cis-isomers of carotenoids (zeaxanthin and beta-carotene) on the thermotropic properties of lipid membranes formed with dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC) were investigated by means of differential scanning calorimetry. The results were compared with the effects caused by the all-trans-isomer. Both the trans and cis isomers of zeaxanthin shifted the main phase transition temperature to lower values and decreased the cooperativity of the phase transition. The effect of all-trans zeaxanthin on the physical properties of the lipid bilayers has been shown to strongly depend on the hydrocarbon chain length of the membrane. In the case of cis-zeaxanthin this relationship is weaker.
Widomska
Justyna
J
Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Cracow 30-387, Poland. jwidomska@gmail.com
Kostecka-Gugała
Anna
A
Latowski
Dariusz
D
Gruszecki
Wiesław I
WI
Strzałka
Kazimierz
K
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002338:Carotenoids / Q000737:chemistry* / Q000378:metabolism; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D007536:Isomerism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008560:Membrane Fluidity; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D024341:Xanthophylls / Q000737:chemistry / Q000378:metabolism; D065146:Zeaxanthins
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D024341:Xanthophylls; D065146:Zeaxanthins; D015060:1,2-Dipalmitoylphosphatidylcholine; D002338:Carotenoids; D004134:Dimyristoylphosphatidylcholine