Transport and storage of sputum specimen by using cetylpyridinium chloride for isolation of mycobacteria.
Of the 191 sputum specimens that were collected from pulmonary tuberculosis patients, 78.65% (140/178) specimens were culture positive when processed within 48 h by the NaOH method. The culture positivity in the same specimen that were stored with cetylpyridinium chloride (CPC) and processed after 7-8 days was 70.22% (125/178), whereas those stored without CPC and processed by the NaOH method was 46.62% (83/178). The difference in number of positive cultures obtained before storage and after storage (without CPC) was statistically significant (P = 0.001). Culture positivity by the CPC method was comparable with that of NaOH method before storage and the difference was not statistically significant (P = 0.35).
Pal
Nita
N
Department of Microbiology, SMS Medical College, Jaipur, Rajasthan, India. nitapal@yahoo.com
Sharma
Babita
B
Malhotra
Bharti
B
Rishi
Suman
S
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002594:Cetylpyridinium / Q000494:pharmacology*; D006801:Humans; D050296:Microbial Viability; D009161:Mycobacterium / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
D000890:Anti-Infective Agents; D002594:Cetylpyridinium