Oxidative stress and histopathology damage related to the metabolism of dodecylbenzene sulfonate in Senegalese sole.
Surfactants such as linear alkylbenzene sulfonates (LAS) are widely utilised in the formulation of detergents in commercial products. After use, they pass through waste water treatment plants (WWTP) and are then discharged to aquatic ecosystems, causing risk to aquatic life. The exposure of marine animals to these compounds enhances the production of reactive oxygen species (ROS) with subsequent damage to macromolecules, and produces histological alterations. A flow-through experiment with Senegalese sole (Solea senegalensis) has been devised with the object of correlating the metabolism of LAS including sulfophenylcarboxylic acids (SPCs) by fish with their antioxidant defence system (generation of oxyradicals) and histopathological damage. The generation of intermediate degradation products (SPCs) by the organism, the histopathological responses, the antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione S-transferase (GST)), as well as other kinds of enzyme such as acid and alkaline phosphatases (AcP, ALP), were measured. SPCs from 5ØC(6) to 11ØC(12) were identified and quantified in fish and water; their concentrations differed depending on the sampling moment. In general, the responses found in the enzymes were slight: a decrease in the enzymatic activity in gills and activation in the digestive tract. The evidence of histopathological damage identified was also small; the organism's defensive mechanism against pollutants should enable it to recover easily. A direct relationship was established between biotransformation and the generation of SPCs and ROS. In conclusion, the correct functioning of the antioxidant defence system with absence of large variations, the short-term histopathological damage, and the evidence of SPCs indicate an adequate metabolism of 2-phenyl-C(12)-linear alkylbenzene sulfonates (2ØC(12)LAS) by this specie and non-toxic effects at environmentally realistic levels.
Alvarez-Muñoz
D
D
Dpto. Química Física, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Avd República Saharaui s/n, 11510 Puerto Real, Cádiz, Spain.
Gómez-Parra
A
A
Blasco
J
J
Sarasquete
C
C
González-Mazo
E
E
D000818:Animals; D001557:Benzenesulfonates / Q000378:metabolism / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D005412:Flatfishes / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D001557:Benzenesulfonates; D017382:Reactive Oxygen Species; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C001114:dodecylbenzenesulfonic acid; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase