title: Analysis of chronic lymphotic leukemia transcriptomic profile: differences between molecular subgroups.
abstract: B cell chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder with a variable clinical course. Patients with unmutated IgV(H) gene show a shorter progression-free and overall survival than patients with immunoglobulin heavy chain variable regions (IgV(H)) gene mutated. In addition, BCL6 mutations identify a subgroup of patients with high risk of progression. Gene expression was analysed in 36 early-stage patients using high-density microarrays. Around 150 genes differentially expressed were found according to IgV(H) mutations, whereas no difference was found according to BCL6 mutations. Functional profiling methods allowed us to distinguish KEGG and gene ontology terms showing coordinated gene expression changes across subgroups of CLL. We validated a set of differentially expressed genes according to IgV(H) status, scoring them as putative prognostic markers in CLL. Among them, CRY1, LPL, CD82 and DUSP22 are the ones with at least equal or superior performance to ZAP70 which is actually the most used surrogate marker of IgV(H) status.
authors.affiliation: Molecular Haematology Laboratory, Prince Felipe Research Centre, Valencia, Spain. ejantus@cipf.es
authors.forename: Eloisa
authors.initials: E
authors.lastname: Jantus Lewintre
authors.forename: Cristina
authors.initials: C
authors.lastname: Reinoso Martín
authors.forename: David
authors.initials: D
authors.lastname: Montaner
authors.forename: Miguel
authors.initials: M
authors.lastname: Marín
authors.forename: María
authors.initials: M
authors.lastname: José Terol
authors.forename: Rosa
authors.initials: R
authors.lastname: Farrás
authors.forename: Isabel
authors.initials: I
authors.lastname: Benet
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: Joaquín
authors.initials: J
authors.lastname: Dopazo
authors.forename: Javier
authors.initials: J
authors.lastname: García-Conde
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006262:Health; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains; D051560:Proto-Oncogene Proteins c-bcl-6