[Detection of chromosomal aberration in sporadic colorectal cancer with comparative genomic hybridization].
OBJECTIVE
To investigate the chromosomal aberration in sporadic colorectal carcinoma and its association with clinicopathological features.


METHODS
Comparative genomic hybridization(CGH) was used to screen the changes in the number of DNA sequence copies in 40 sporadic colorectal cancer patients in order to identify regions that contain genes important for the development and progression of colorectal cancer.


RESULTS
In 40 sporadic colorectal cancer, frequent gain at 20 q, 12 q, 13 q, 7 p, 7 q and 16 q were found, while loss was also found at 18 q, 5 q, 4 q, 8 pand 17 p. The number of chromosomal aberration was closely associated with tumor stage(P<0.05). No significant association was found between the number of chromosomal aberration and tumor site, histopathologic type and histologic grade.


CONCLUSIONS
Chromosomal aberration exists generally in sporadic colorectal carcinoma. The number of chromosomal aberration and gain of 20q are closely associated with tumor stage.
Chen
Yin-Bo
YB
Department of Colorectal Cancer, Zhejiang Provincial Cancer Hospital, Hangzhou 310022, China.
Ju
Hai-Xing
HX
Zhang
Su-Zhan
SZ
Peng
Jia-Ping
JP
Li
De-Chuan
DC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D055028:Comparative Genomic Hybridization; D015342:DNA Probes; D005260:Female; D018628:Gene Dosage; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis
D015342:DNA Probes