[The analysis of human immunodeficiency virus-1 subtypes in Yunnan province].
OBJECTIVE
To analyze the geographical distribution and risk factors of HIV-1 subtypes in Yunnan province.


METHODS
Blood samples from 1319 HIV positives were collected in Yunnan Province from 2001 to 2006. The nested polymerase chain reaction was used to amplify the gag (p24)-protease fragments from RNA extracted from plasma or sera. The sequences were used for subtype determination by phylogenetic tree analysis.


RESULTS
Among 1319 samples studied, the subtypes has been successfully obtained from 644 samples that were constituted of seven subtypes: CRF08_BC, CRF07_BC, CRF07/08_BC, CRF01_AE, C, B' and URFB/C. C/CRF07_BC/CRF08_BC were distributed in the whole province, but CRF01_AE were mainly distributed in the boarding areas with Myanmar such as Dehong, Baoshan, Xishuangbanna and Puer. Moreover, injecting drugs users accounted for 61.6% (270/438) among C/CRF07_BC/CRF08_BC infections, while only 8.5% (15/177) among CRF01_AE infections.


CONCLUSIONS
Our data indicated that at least seven subtypes were identified in Yunnan province, the relationship between subtypes and transmission routes were analyzed, and the geographic difference of subtypes was also observed.
Ma
Yan-ling
YL
Center for HIV/AIDS Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming 650022, China.
Zhang
Yong
Y
Lu
Lin
L
Yang
Li
L
Shi
Yu-hua
YH
Yang
Chao-jun
CJ
Luo
Hong-bing
HB
Yan
Wen-yun
WY
Min
Xiang-dong
XD
Su
Ying-zhen
YZ
Chen
Ling
L
Qiao
En-fa
EF
Chen
Hui-chao
HC
Cui
Wen-qing
WQ
Liu
Fang
F
Jia
Man-hong
MH
D002681:China; D004279:DNA, Viral; D005838:Genotype; D015658:HIV Infections / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D017422:Sequence Analysis, DNA
D004279:DNA, Viral