High-sensitive glutamate biosensor based on NADH at Lauth's violet/multiwalled carbon nanotubes composite film on gold substrates.
A highly sensitive amperometric L-glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide has been developed on Lauth's Violet (known as thionine)/multiwalled carbon nanotubes (Th-MWCNTs) composite film, which is used as a mediator and an enzyme immobilization matrix. The glutamate biosensor, which is fabricated by immobilizing glutamate dehydrogenase (GLDH) on the surface of Th-MWCNTs, displayed a precipitous response (ca. 3 s), a low detection limit (15.9 nM), a wide linear dynamic range (0.1 to 500 microM), and high sensitivity of 281.6 microAmM(-1) cm(-2), higher biological affinity, as well as good stability and repeatability. Interferences from other biological compounds were also studied for the fabricated sensor. The Th-MWCNTs system exemplifies a simple and efficient approach to the assimilation of GLDH and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications in health care fields.
Rahman
M M
MM
Venture Business Laboratory, Department of Electrical and Electronic Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan. ahmadumar@chonbuk.ac.kr
Umar
Ahmad
A
Sawada
Kazuaki
K
D015374:Biosensing Techniques; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis* / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009243:NAD / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure
D037742:Nanotubes, Carbon; D009243:NAD; D018698:Glutamic Acid; D006046:Gold; D005969:Glutamate Dehydrogenase