SCARF: maximizing next-generation EST assemblies for evolutionary and population genomic analyses.
CONCLUSIONS
Scaffolded and Corrected Assembly of Roche 454 (SCARF) is a next-generation sequence assembly tool for evolutionary genomics that is designed especially for assembling 454 EST sequences against high-quality reference sequences from related species. The program was created to knit together 454 contigs that do not assemble during traditional de novo assembly, using a reference sequence library to orient the 454 sequences.


BACKGROUND
SCARF is freely available at http://msbarker.com/software.htm, and is released under the open source GPLv3 license (http://www.opensource.org/licenses/gpl-3.0.html.
Barker
Michael S
MS
Botany Department, University of British Columbia, Vancouver, B.C. V6T 1Z4, Canada. msbarker@indiana.edu
Dlugosch
Katrina M
KM
Reddy
A Chaitanya C
AC
Amyotte
Sarah N
SN
Rieseberg
Loren H
LH
D031179:Centaurea / Q000235:genetics; D030541:Databases, Genetic; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D016678:Genome; D023281:Genomics / Q000379:methods*; D012984:Software