title: Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression.
abstract: OBJECTIVE
  To investigate the role of the Notch1 signaling pathway in growth arrest of an esophageal carcinoma cell line (EC109) in vitro and the mechanism involved.
  
  
  METHODS
  An intracellular domain of Notch1 (ICN) was transfected into cultured EC109 cells by lipofectamine transfection. Subsequently, the proliferation of the transfected cells was measured by an MTT assay. Cell cycle distribution was analyzed by flow cytometry. Human papillomavirus type 18 (HPV18) E6/E7 mRNA expression was detected by RT-PCR, and p53 protein expression was detected by Western blot.
  
  
  RESULTS
  Activation of Notch1 signaling resulted in inhibition of EC109 cell proliferation with the induction of G(2)/M arrest, downmodulation of HPV18 E6/E7 gene expression, and upregulation of p53 expression.
  
  
  CONCLUSIONS
  Repression of HPV18 E6/E7 expression by Notch1 signaling results in the activation of p53-mediated pathways with concomitant growth suppression of HPV18-positive EC109 cells.
authors.affiliation: Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Ke-Jie
authors.initials: KJ
authors.lastname: Zhang
authors.forename: Quan-Yi
authors.initials: QY
authors.lastname: Lu
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Niu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Jiang-Ning
authors.initials: JN
authors.lastname: Zhao
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Jia-Sheng
authors.initials: JS
authors.lastname: Hu
authors.forename: Pu
authors.initials: P
authors.lastname: Li
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Liu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; C497761:NOTCH1 protein, human; D009856:Oncogene Proteins, Viral; D051881:Receptor, Notch1; D016159:Tumor Suppressor Protein p53