Vertical alveolar ridge augmentation with beta-tricalcium phosphate and autologous osteoblasts in canine mandible.
A tissue-engineered bone has become a viable alternative to autologous bone for bone augmentation in atrophy alveolar ridge. The aim of the present study was to evaluate porous beta-tricalcium phosphate (beta-TCP) combined with autologous osteoblasts to augment edentulous alveolar ridge in a canine model. Autologous osteoblasts were expanded and combined with beta-TCP scaffold to fabricate a tissue-engineered bone. 12 bilateral alveolar ridge augmentation surgeries were carried out in 6 beagle dogs with the following 3 groups: beta-TCP/osteoblasts, beta-TCP alone and autogenous iliac bone control (n=4 per group). Sequential fluorescent labeling and radiographs were used to compare new bone formation and mineralization in each group. 24 weeks later, animals were sacrificed and non-decalcified and decalcified sections were evaluated histologically and histomorphometrically. Results indicated that the tissue-engineered bone dramatically enhanced new bone formation and mineralization, increase the new bone area, and maintain the height and thickness of the augmented alveolar ridge when compared with beta-TCP alone group. More importantly, the tissue-engineered bone achieved an elevated bone height and thickness comparable to that of autogenous iliac bone graft. This study demonstrated the potential of porous beta-TCP as a substrate for autogenous osteoblasts in bone tissue engineering for alveolar ridge augmentation.
Wang
Shaoyi
S
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Zhang
Zhiyuan
Z
Zhao
Jun
J
Zhang
Xiuli
X
Sun
Xiaojuan
X
Xia
Lunguo
L
Chang
Qing
Q
Ye
Dongxia
D
Jiang
Xinquan
X
D000540:Alveolar Ridge Augmentation; D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D004285:Dogs; D008334:Mandible / Q000166:cytology* / Q000378:metabolism* / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype
D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate