title: Assessment of long- and short-term neurotoxic effects of glass ionomer bone cement by electromyography and histopathologic examination: experimental study.
abstract: OBJECTIVE
  The objective of this study was to investigate the possible neurotoxic effects of bone cement on the peripheral nerves.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Teaching and research hospital.
  
  
  METHODS
  Twenty New Zealand rabbits were included in this study. The sciatic nerves of both legs of the 10 rabbits were exposed surgically under general anesthesia and closed primarily without any intervention and constituted the control group (group 1). Following surgical exploration, glass ionomer cement (GIC) was applied to the left sciatic nerves of the 10 rabbits for 10 seconds and then aspirated (group 2). GIC material was also applied to the right sciatic nerves of these rabbits but without aspiration (group 3).
  
  
  METHODS
  All rabbits were sacrificed at the end of 8 weeks postoperatively following electromyographic investigation. The sections were stained with hematoxylin-eosin and Immune Olig 2 staining technique for histopathologic examination under light microscopy.
  
  
  RESULTS
  There was no statistically significant difference in distal latency, which indicates the conduction speed of the nerve, between all groups by electromyography. Histopathologic examination of all specimens revealed no demyelinization or axonal degeneration, and all had an intact myelin structure. There was no statistically significant difference in inflammation of the specimens between groups. (p>.05).
  
  
  CONCLUSIONS
  GIC has no neurotoxic effects on the nerves in short- and long-term applications.
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, Sişli Etfal Teaching and Research Hospital, and Department of Neurology, Istanbul University Medicine Faculty, Istanbul, Turkey. seyhanalkan@hotmail.com
authors.forename: Seyhan
authors.initials: S
authors.lastname: Alkan
authors.forename: Alper Tolga
authors.initials: AT
authors.lastname: Koşar
authors.forename: Esra
authors.initials: E
authors.lastname: Sozen
authors.forename: Serdar
authors.initials: S
authors.lastname: Baylançiçek
authors.forename: Bariş
authors.initials: B
authors.lastname: Baslo
authors.forename: Tülay
authors.initials: T
authors.lastname: Başak
authors.forename: Burhan
authors.initials: B
authors.lastname: Dadaş
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004558:Electric Stimulation; D004576:Electromyography; D005899:Glass Ionomer Cements / Q000494:pharmacology*; D009431:Neural Conduction / Q000187:drug effects; D011817:Rabbits; D011930:Reaction Time / Q000187:drug effects; D012584:Sciatic Nerve / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D013396:Suction
chemical_list: D005899:Glass Ionomer Cements