title: Polyanion-induced self-association of complement factor H.
abstract: Factor H is the primary soluble regulator of activation of the alternative pathway of complement. It prevents activation of complement on host cells and tissues upon association with C3b and surface polyanions such as sialic acids, heparin, and other glycosaminoglycans. Here we show that interaction with polyanions causes self-association forming tetramers of the 155,000 Da glycosylated protein. Monomeric human factor H is an extended flexible protein that exhibits an apparent size of 330,000 Da, relative to globular standards, during gel filtration chromatography in the absence of polyanions. In the presence of dextran sulfate (5000 Da) or heparin an intermediate species of apparent m.w. 700,000 and a limit species of m.w. 1,400,000 were observed by gel filtration. Sedimentation equilibrium analysis by analytical ultracentrifugation indicated a monomer Mr of 163,000 in the absence of polyanions and a Mr of 607,000, corresponding to a tetramer, in the presence of less than a 2-fold molar excess of dextran sulfate. Increasing concentrations of dextran sulfate increased binding of factor H to zymosan-C3b 4.5-fold. This result was accompanied by an increase in both the decay accelerating and cofactor activity of factor H on these cells. An expressed fragment encompassing the C-terminal polyanion binding site (complement control protein domains 18-20) also exhibited polyanion-induced self-association, suggesting that the C-terminal ends of factor H mediate self-association. The results suggest that recognition of polyanionic markers on host cells and tissues by factor H, and the resulting regulation of complement activation, may involve formation of dimers and tetramers of factor H.
authors.affiliation: Department of Biochemistry, Center for Biomedical Research, University of Texas Health Science Center, Tyler, TX 75708, USA. michael.pangburn@uthct.edu
authors.forename: Michael K
authors.initials: MK
authors.lastname: Pangburn
authors.forename: Nenoo
authors.initials: N
authors.lastname: Rawal
authors.forename: Claudio
authors.initials: C
authors.lastname: Cortes
authors.forename: M Nurul
authors.initials: MN
authors.lastname: Alam
authors.forename: Viviana P
authors.initials: VP
authors.lastname: Ferreira
authors.forename: Mark A L
authors.initials: MA
authors.lastname: Atkinson
mesh_terms: D000838:Anions / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics / Q000276:immunology; D002850:Chromatography, Gel; D017242:Complement Factor H / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016264:Dextran Sulfate / Q000737:chemistry; D019281:Dimerization; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D013816:Thermodynamics
chemical_list: D000838:Anions; D017242:Complement Factor H; D016264:Dextran Sulfate; D010446:Peptide Fragments; D000071228:Polyelectrolytes; D011108:Polymers; C493495:CFH protein, human