New gas chromatographic method for the enantioseparation of beta-amino acids by a rapid double derivatization technique.
A novel gas chromatographic method was developed for the enantioseparation of valuable acyclic and carbocyclic cis- and trans-beta-amino acids, including cispentacin and a number of its analogues and homologues. Excellent (in most cases baseline) separation was achieved for the racemates of these beta-amino acids on CP-Chirasil-Dex CB or CP-Chirasil L-Val columns after a simple and rapid double derivatization (esterification followed by N-acylation). The elution sequences were determined in all cases.
Forró
Eniko
E
Institute of Pharmaceutical Chemistry, University of Szeged, Eötvös utca 6, H-6720 Szeged, Hungary. Forro.Eniko@pharm.u-szeged.hu
D000598:Amino Acids, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D002849:Chromatography, Gas / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000302:isolation & purification; D009930:Organic Chemicals / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012833:Siloxanes / Q000737:chemistry; D013237:Stereoisomerism
D000598:Amino Acids, Cyclic; C041118:Chirasil-Val; D003505:Cyclodextrins; D009930:Organic Chemicals; D012833:Siloxanes; D003515:Cycloleucine; C090733:Chirasil-Dex; C065594:cispentacin