title: Temporal phases of activity-dependent plasticity and memory are mediated by compartmentalized routing of MAPK signaling in aplysia sensory neurons.
abstract: An activity-dependent form of intermediate memory (AD-ITM) for sensitization is induced in Aplysia by a single tail shock that gives rise to plastic changes (AD-ITF) in tail sensory neurons (SNs) via the interaction of action potential firing in the SN coupled with the release of serotonin in the CNS. Activity-dependent long-term facilitation (AD-LTF, lasting >24hr) requires protein synthesis dependent persistent mitogen-activated protein kinase (MAPK) activation and translocation to the SN nucleus. We now show that the induction of the earlier temporal phase (AD-ITM and AD-ITF), which is translation and transcription independent, requires the activation of a compartmentally distinct novel signaling cascade that links second messengers, MAPK and PKC into a unified pathway within tail SNs. Since both AD-ITM and AD-LTM require MAPK activity, these collective findings suggest that presynaptic SNs route the flow of molecular information to distinct subcellular compartments during the induction of activity-dependent long-lasting memories.
authors.affiliation: Department of Neurobiology and Behavior, University of California, Irvine, Irvine, CA 92697, USA.
authors.forename: Justin L
authors.initials: JL
authors.lastname: Shobe
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shara
authors.initials: S
authors.lastname: Stough
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ye
authors.forename: Vickie
authors.initials: V
authors.lastname: Hsuan
authors.forename: Kelsey C
authors.initials: KC
authors.lastname: Martin
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Carew
mesh_terms: D000818:Animals; D001048:Aplysia / Q000033:anatomy & histology / Q000502:physiology; D003214:Conditioning, Classical / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000166:cytology / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
chemical_list: D012701:Serotonin; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; D019204:GTP-Binding Proteins