title: Increased potency and longevity of gene silencing using validated Dicer substrates.
abstract: Chemically synthesized small interfering RNAs (siRNAs) are tools used for silencing the expression of a single gene. They are mainly employed in basic research applications, but may also have great potential in therapeutic applications. Longer double-stranded RNAs, such as Dicer-substrate 27mers, trigger gene silencing through the intrinsic RNAi pathway. The design of these Dicer-substrate 27mers has been optimized so they can be oriented by Dicer to consistently select the antisense (guide) strand after cleavage to shorter siRNAs, leading to predictable mRNA cleavage. In this paper we describe evidence that these Dicer-substrate 27mers produce more potent and sustained gene silencing for four genes when compared with synthetic 21mers that have the same guide-strand sequence. Furthermore, improved silencing by these 27mers is often more pronounced at lower concentrations.
authors.affiliation: Bio-Rad Laboratories, Inc., Hercules, CA 94547, USA. eli_hefner@bio-rad.com
authors.forename: E
authors.initials: E
authors.lastname: Hefner
authors.forename: K
authors.initials: K
authors.lastname: Clark
authors.forename: C
authors.initials: C
authors.lastname: Whitman
authors.forename: M A
authors.initials: MA
authors.lastname: Behlke
authors.forename: S D
authors.initials: SD
authors.lastname: Rose
authors.forename: A S
authors.initials: AS
authors.lastname: Peek
authors.forename: T
authors.initials: T
authors.lastname: Rubio
mesh_terms: D001483:Base Sequence; D001709:Biotechnology; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D020868:Gene Silencing; D016158:Genes, p53; D006367:HeLa Cells; D006801:Humans; D008957:Models, Genetic; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors; D014162:Transfection
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt; D019908:Proto-Oncogene Proteins c-raf; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D043244:Ribonuclease III