title: Ginsenoside Rp1, a ginsenoside derivative, blocks lipopolysaccharide-induced interleukin-1beta production via suppression of the NF-kappaB pathway.
abstract: Ginsenoside Rp1 (G-Rp1) is a ginseng saponin derivative with chemopreventive and anti-cancer activities. In this study, we examined the regulatory activity of G-Rp1 on the production of interleukin (IL)-1beta, a pro-inflammatory cytokine managing acute or chronic inflammatory diseases such as septic shock and rheumatoid arthritis, from lipopolysaccharide (LPS)-treated macrophage-like RAW264.7 cells. G-Rp1 dose-dependently inhibited IL-1beta production from LPS-treated RAW264.7 cells without altering cell viability. This compound suppressed both mRNA and protein levels of IL-1beta. In particular, this compound was found to down-regulate phosphorylation of the inhibitor of kappaB (IkappaB) kinase (IKK)/IkappaBalpha, and consequent activation of NF-kappaB, but not the activation of its upstream signaling enzymes such as mitogen-activated protein kinases (MAPK) and p85, a regulatory subunit of phosphoinositide 3-kinase (PI3K). Therefore, these results suggest that G-Rp1 may act as an inhibitor of IL-1beta production by inhibiting the NF-kappaB pathway.
authors.affiliation: School of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea.
authors.forename: Byung Hun
authors.initials: BH
authors.lastname: Kim
authors.forename: Yong Gyu
authors.initials: YG
authors.lastname: Lee
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: Park
authors.forename: Ho Bang
authors.initials: HB
authors.lastname: Kim
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000187:drug effects; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D036145:Ginsenosides; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; C512675:ginsenoside Rp1; C082161:lipopolysaccharide A