title: Factors responsible for plaque formation of A/duck/Siberia/272/1998 (H13N6) influenza virus on MDCK cells.
abstract: Many influenza A viruses form plaques on Madin-Darby canine kidney (MDCK) cells in the presence of trypsin. A/duck/Siberia/272/1998 (H13N6) (Sib272), however, does not form plaque on MDCK cells. After three blind passages of the strain on MDCK cells, plaque-forming variant was obtained and designated as A/duck/Siberia/272PF/1998 (H13N6) (Sib272PF). Genetic and functional analyses of Sib272 and Sib272PF revealed that amino acid substitutions, F3L of the HA2 subunit and T379K of the PB1, were responsible for plaque formation of Sib272PF by enhancing fusion and polymerase activities, respectively.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Nishi-9, Kita-18, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Norikazu
authors.initials: N
authors.lastname: Isoda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development; D009976:Orthomyxoviridae Infections / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017423:Sequence Analysis, RNA; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics*; D014776:Virus Cultivation
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C057027:PB2 protein, Influenzavirus A; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase