Isoflavone regulates lipid metabolism via expression of related genes in OVX rats fed on a high-fat diet.
OBJECTIVE
To investigate the effects of isoflavone on body weight, fat mass, and gene expression in relation to lipid metabolism.


METHODS
Thirty-six female SD rats were ovariectomized or sham-operated and fed on a high-fat diet. Two months later, abdominal incision was made, blood was collected to separate serum, and the liver and adipose tissue were immediately collected and weighed. Some portions of these tissues were frozen in liquid nitrogen and stored at -80 degrees C.


RESULTS
Ovariectomy (OVX) with a high-fat diet could induce obesity in rats, while treatment with isoflavone significantly inhibited the increase in body weight and fat mass in abdomen. Serum total cholesterol and leptin were significantly decreased in isoflavone group, compared with the OVX group. The mRNA expression of liver fatty acid synthase (FAS) in the OVX group was significantly higher than that in sham-operated group, while this difference was not observed in the isoflavone group. The mRNA expression of liver hormone-sensitive lipase (HSL) in the OVX rats tended to be lower than that in the sham-operated rats. Furthermore, a large amount of isoflavone maintained the mRNA expression at a sham level.


CONCLUSIONS
Isoflavone may prevent obesity induced by ovariectomy with a high-fat diet, in part by modulating gene expression related to lipid metabolism.
Na
Xiao-Lin
XL
Department of Food Nutrition and Hygiene, Public Health College, Harbin Medical University, Harbin 150086, Heilongjiang, China.
Ezaki
Junko
J
Sugiyama
Fumie
F
Cui
Hong-Bin
HB
Ishimi
Yoshiko
Y
D000273:Adipose Tissue; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D004041:Dietary Fats / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000033:anatomy & histology; D009929:Organ Size; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
D004041:Dietary Fats; D007529:Isoflavones