Candida albicans gene expression in an in vivo infection model.
A general procedure is described for the analysis of gene expression of Candida albicans cultured in a mouse infection model. This technique involves first infecting mice with Candida and subsequently harvesting blood and other tissue at specific time points during infection. The tissues are homogenized and the infecting Candida isolated. Finally, RNA is extracted from recovered Candida cells and subjected to microarray analysis.
Kruppa
Michael D
MD
Department of Microbiology, East Tennessee State University, James H Quillen College of Medicine, Johnson City, TN, USA.
D000818:Animals; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D002177:Candidiasis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods