Quantitative proteomic analysis of differential proteins in the stroma of nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissue.
The importance of stromal cells and the factors that they expressed during cancer initiation and progression have been highlighted by recent literature. To identify the stromal proteins involved in nasopharyngeal carcinoma (NPC) carcinogenesis, we assessed differences in protein expression of the stroma from NPC and normal nasopharyngeal epithelium tissues (NNET) using a quantitative proteomic approach combined with laser capture microdissection (LCM). LCM was performed to purify stromal cells from the NPC and NNET, respectively. The differential proteins between the pooled microdissected tumor and normal stroma were analyzed by two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS). Twenty differential proteins were identified, and the expression and location of two differential proteins (L-plastin and S100A9) were further confirmed by Western blotting and immunohistochemical analysis. Our results will be helpful to study the role of stroma in the NPC carcinogenesis, as well as discover the interaction between NPC cells and their surrounding microenvironment.
Li
Mei-Xiang
MX
Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Xiao
Zhi-Qiang
ZQ
Liu
Ying-Fu
YF
Chen
Yong-Heng
YH
Li
Cui
C
Zhang
Peng-Fei
PF
Li
Mao-Yu
MY
Li
Feng
F
Peng
Fang
F
Duan
Chao-Jun
CJ
Yi
Hong
H
Yao
Hui-Xin
HX
Chen
Zhu-Chu
ZC
D040502:Calgranulin B / Q000032:analysis*; D003238:Connective Tissue / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008562:Membrane Glycoproteins / Q000032:analysis*; D008840:Microfilament Proteins / Q000032:analysis*; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry* / Q000473:pathology; D009305:Nasopharynx / Q000737:chemistry / Q000166:cytology; D009363:Neoplasm Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D040502:Calgranulin B; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; C026742:plastin