title: Hemoglobin E detection using PCR with confronting two-pair primers.
abstract: OBJECTIVE
  To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E).
  
  
  METHODS
  Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.
  
  
  RESULTS
  The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively.
  
  
  CONCLUSIONS
  Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations.
authors.affiliation: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Indrawaroros Rd, Sripoom, Mueang, Chiang Mai 50200, Thailand. isorasak@hotmail.com
authors.forename: Sorasak
authors.initials: S
authors.lastname: Intorasoot
authors.forename: Rachanu
authors.initials: R
authors.lastname: Thongpung
authors.forename: Khajornsak
authors.initials: K
authors.lastname: Tragoolpua
authors.forename: Mongkol
authors.initials: M
authors.lastname: Chottayaporn
mesh_terms: D004247:DNA; D017931:DNA Primers; D005784:Gene Amplification; D005838:Genotype; D006446:Hemoglobin E / Q000032:analysis* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D013785:Thailand; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000235:genetics
chemical_list: D017931:DNA Primers; D004247:DNA; D006446:Hemoglobin E