Anti-hepatitis B virus activities of astragaloside IV isolated from radix Astragali.
Total ethanol extract and saponins from Chinese herb radix Astragali (huangqi) have been previously shown to possess anti-hepatitis B virus (HBV) activities in vitro. To identify the active ingredients, we isolated a triterpenoid saponin that was determined to be astragaloside IV. In the human HBV-transfected liver cell line HepG(2) 2.2.15, astragaloside IV effectively suppressed secretion of HBV antigens with inhibition rates of 23.6% for the secretion of Hepatitis B surface antigen (HBsAg) and 22.9% for that of Hepatitis B e antigen (HBeAg) at 100 microg/ml after 9 d of treatment. The inhibitory activity of astragaloside IV on secretion of HBV antigens is more potent than that of 3TC without significant cytotoxicity. In duck hepatitis B virus (DHBV)-infected ducklings, astragaloside IV caused 64.0% inhibition at 120 mg/kg, 49.6% inhibition at 40 mg/kg, and 41.7% inhibition at 10 mg/kg to serum DHBVs after 10 d of treatment, and also reduced serum DHBV DNA levels. Together, our results demonstrate that astragaloside IV possesses potent anti-HBV activity.
Wang
Shuguang
S
Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry.
Li
Jiyang
J
Huang
Hai
H
Gao
Wen
W
Zhuang
Changlong
C
Li
Bo
B
Zhou
Pei
P
Kong
Deyun
D
D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry*; D002277:Carcinoma; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004372:Ducks; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D015611:Hepatitis B Virus, Duck / Q000187:drug effects / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D012503:Saponins / Q000494:pharmacology*; D014162:Transfection / Q000379:methods; D014315:Triterpenes / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A