ApoC-III gene expression is sharply increased during adipogenesis and is augmented by retinoid X receptor (RXR) agonists.
ApoC-III, a lipoprotein lipase inhibitor and a key regulator of the metabolism of triglyceride-rich lipoproteins, is mainly synthesized in liver and intestine, but little is known about its expression in adipocytes. We here show that apoC-III mRNA levels are dramatically induced during adipocyte differentiation. Among many transcriptional factors involved in adipocyte differentiation, RXRalpha, acting alone and not as a heterodimer partner of other nuclear receptors, solely regulates apoC-III gene expression. These results suggest that apoC-III may play a specific role in lipid storage and mobilization in adipocytes, non-lipoprotein-secreting cells, and indicate the functional role of RXRalpha during adipocyte differentiation.
Takahashi
Yu
Y
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Inoue
Jun
J
Kagechika
Hiroyuki
H
Sato
Ryuichiro
R
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D053305:Apolipoprotein C-III / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D051379:Mice; D047490:Retinoid X Receptor alpha / Q000819:agonists / Q000378:metabolism*
D053305:Apolipoprotein C-III; D047490:Retinoid X Receptor alpha