Reconstructing mandibular defects using autologous tissue-engineered tooth and bone constructs.
OBJECTIVE
Current strategies for jaw reconstruction require multiple operations to replace bone and teeth. To improve on these methods, we investigated simultaneous mandibular and tooth reconstruction, using a Yucatan minipig model.


METHODS
Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic (VCT), histological, and immunohistochemical analyses.


RESULTS
Small tooth structures were identified, and consisted of organized dentin, enamel, pulp, and periodontal ligament tissues, surrounded by new bone. No dental tissues formed in implants without tooth-bud cells, and bone regeneration was observed to a limited extent. Immunohistochemical analyses using tooth-specific and bone-specific antibodies confirmed the identity of regenerated tissues.


CONCLUSIONS
This pilot study supports the feasibility of tissue-engineering approaches for coordinated autologous tooth and mandible reconstruction, and provides a basis for future improvement of this technique for eventual clinical use in humans.
Abukawa
Harutsugi
H
Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, USA.
Zhang
Weibo
W
Young
Conan S
CS
Asrican
Rose
R
Vacanti
Joseph P
JP
Kaban
Leonard B
LB
Troulis
Maria J
MJ
Yelick
Pamela C
PC
D053523:Amelogenin / Q000096:biosynthesis; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000637:transplantation; D005240:Feasibility Studies; D008297:Male; D008334:Mandible / Q000166:cytology / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D023421:Models, Animal; D009805:Odontogenesis / Q000502:physiology*; D010006:Osteoblasts / Q000637:transplantation*; D010750:Phosphoproteins / Q000096:biosynthesis; D010865:Pilot Projects; D012038:Regeneration / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014070:Tooth / Q000166:cytology / Q000378:metabolism; D014083:Tooth Germ / Q000637:transplantation*
D053523:Amelogenin; D010750:Phosphoproteins