title: Assessment of CE for the identification of microorganisms.
abstract: The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris-citrate-fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post-separation cultivation and sequential analysis was developed. Off-line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, Palacký University in Olomouc, Olomouc, Czech Republic. petrjan1@gmail.com
authors.forename: Jan
authors.initials: J
authors.lastname: Petr
authors.forename: Olga
authors.initials: O
authors.lastname: Ryparová
authors.forename: Václav
authors.initials: V
authors.lastname: Ranc
authors.forename: Petra
authors.initials: P
authors.lastname: Hinnerová
authors.forename: Joanna
authors.initials: J
authors.lastname: Znaleziona
authors.forename: Marta
authors.initials: M
authors.lastname: Kowalska
authors.forename: Radim
authors.initials: R
authors.lastname: Knob
authors.forename: Vítezslav
authors.initials: V
authors.lastname: Maier
authors.forename: Ivo
authors.initials: I
authors.lastname: Frébort
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
authors.forename: Juraj
authors.initials: J
authors.lastname: Sevcík
mesh_terms: D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014325:Tromethamine / Q000737:chemistry
chemical_list: D004573:Electrolytes; D014325:Tromethamine; D011092:Polyethylene Glycols