Clinical implications of quantitative real time-polymerase chain reaction of parvovirus B19 in kidney transplant recipients - a prospective study.
This prospective study was designed to investigate the clinically significant level of parvovirus B19 viral load using quantitative real-time (RT) polymerase-chain reaction (PCR) in kidney transplantation (KT) recipients. One hundred forty-three adult recipients who underwent their first KT between November 2003 and October 2005 were enrolled. Six blood samples (the first taken preoperatively, subsequent samples taken every 4 weeks for 20 weeks) were taken from each patient for parvovirus B19 DNA RT-PCR analysis. All recipients were diligently followed for 1 year post-transplant. One hundred sixty-eight of the 715 (23.5%) postoperative samples were positive for parvovirus B19 PCR. Eighty-four of the 143 KT recipients (58.7%) showed at least one positive PCR. Sixteen of the 143 (11.1%) KT recipients had sustained severe anemia (SSA) with hemoglobin lower than 7.0 g/dl, after 4 weeks post-transplant. The incidence of SSA in recipients with a titer higher than 1 x 10(6) copies/5 microl whole blood was significantly higher than those with a negative or low titer (P < 0.001, positive predictive value 84.6%, negative predictive value 96.2%). In conclusion, a high titer of parvovirus B19 DNA higher than 1 x 10(6) copies/5 microl whole blood in KT recipients was related with SSA after 4 weeks post-transplant.
Park
Jae Berm
JB
Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Kim
Doo-Jin
DJ
Woo
Sook-Young
SY
Choi
Gyu-Seong
GS
Chun
Jae Min
JM
Jung
Gum O
GO
Kwon
Choon Hyuck D
CH
Kim
Sung-Joo
SJ
Joh
Jae-Won
JW
Lee
Suk-Koo
SK
D000328:Adult; D000740:Anemia / Q000453:epidemiology / Q000209:etiology*; D015897:Comorbidity; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D015994:Incidence; D016030:Kidney Transplantation / Q000009:adverse effects*; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D019562:Viral Load / Q000379:methods*
D004279:DNA, Viral; D006454:Hemoglobins