title: [Risk assessment for environmental release of genetically engineered microorganism m-CDS-1].
abstract: OBJECTIVE
  To assess the environmental release risk of genetically engineered microorganism Sphingomonas sp. m-CDS-1.
  
  
  METHODS
  Pesticides detection, plates counting, most probable number-PCR (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) were used to study the pesticides degrading effects, the decline dynamics of m-CDS-1, and the effects of release of m-CDS-1 on community structure of soil microorganisms.
  
  
  RESULTS
  m-CDS-1 (1.01 x 10(7)CFU/g dry soil) could degrade both 10.71 mg/kg methyl parathion and 1.29 mg/kg carbofuran in 30 days. m-CDS-1 declined quickly with time, and could not be detected anymore in 30 days. Release of m-CDS-1 would not change the number of the cultivable microorganisms notably. The similarities of PCR-DGGE profiles between the pesticides applied treatments and the controls in 4, 11 and 30 days were 17.16 %, 49.81 % and 75.01 %, and the similarities between the m-CDS-1 released treatments and controls were 49.57 %, 38.3 % and 83.3 %, respectively. In 60 days, DGGE profiles of all treatments and controls had high similarities.
  
  
  CONCLUSIONS
  m-CDS-1 could degrade both pesticides remarkably in the field, and would not be the predominant population, and would not affect the community structure of soil microorganisms in the long term.
authors.affiliation: College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. jiang_jjd@njau.edu.cn
authors.forename: Jiandong
authors.initials: J
authors.lastname: Jiang
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Guo
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
mesh_terms: D001673:Biodegradation, Environmental; D017753:Ecosystem; D005818:Genetic Engineering; D008743:Methyl Parathion / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000378:metabolism* / Q000502:physiology; D010575:Pesticides / Q000378:metabolism; D018570:Risk Assessment; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology / Q000592:standards; D012989:Soil Pollutants / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics / Q000378:metabolism*
chemical_list: D010575:Pesticides; D012987:Soil; D012989:Soil Pollutants; D008743:Methyl Parathion