Glycomic approach for potential biomarkers on prostate cancer: profiling of N-linked glycans in human sera and pRNS cell lines.
Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.
de Leoz
Maria Lorna A
ML
Department of Chemistry, University of California Davis, Davis, CA, USA.
An
Hyun Joo
HJ
Kronewitter
Scott
S
Kim
Jaehan
J
Beecroft
Sean
S
Vinall
Ruth
R
Miyamoto
Suzanne
S
de Vere White
Ralph
R
Lam
Kit S
KS
Lebrilla
Carlito
C
D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism; D005583:Fourier Analysis; D020869:Gene Expression Profiling; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D011134:Polysaccharides; D017430:Prostate-Specific Antigen; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase