title: The effect of fetal tracheal occlusion on lung tissue mechanics and tissue composition.
abstract: Fetal tracheal occlusion (TO) is currently used to treat severe cases of congenital diaphragmatic hernia (DH). Clinical and experimental studies suggest an improved postnatal outcome, but lung tissue mechanics after TO have not been studied. We determined the effect of TO on mechanical impedance and lung tissue components in a rabbit model for DH. At 23 days of gestation (term = 31 days) either a sham thoracotomy or a diaphragmatic defect was induced. DH fetuses were randomly assigned to undergo 5 days later TO. Fetuses were delivered by term cesarean section to determine lung to body weight ratio (LBWR), dynamic lung mechanics and lung impedance. Airway resistance (R(aw)), elastance (H(L)), tissue damping (G(L)) and hysteresivity (G(L)/H(L)) were calculated from impedance data. Collagen I and III and elastin were quantified histologically. LBWR was significantly increased by TO compared to DH (P < 0.001) and resistance and compliance of the respiratory system (R(rs), C(rs)) were improved as well. TO resulted in a significant decrease of R(aw) comparable to observations in sham-fetuses, without effect on lung tissue mechanics H(L), G(L) and hysteresivity. This coincides with a significant decrease of collagen I, III and elastin in comparison to DH fetuses. In this first report on lung tissue mechanics in a rabbit model of DH, TO had a substantial effect on tissue morphology yet this was not mirrored in lung mechanics. We conclude that the effect of TO on lung mechanics without in utero reversal of occlusion, is dominated by airway remodeling.
authors.affiliation: Faculty of Medicine, Center for Surgical Technologies, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Jacques C
authors.initials: JC
authors.lastname: Jani
authors.forename: Andreas W
authors.initials: AW
authors.lastname: Flemmer
authors.forename: Florian
authors.initials: F
authors.lastname: Bergmann
authors.forename: Denis
authors.initials: D
authors.lastname: Gallot
authors.forename: Xenia
authors.initials: X
authors.lastname: Roubliova
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Muensterer
authors.forename: Kerstin
authors.initials: K
authors.lastname: Hajek
authors.forename: Jan A
authors.initials: JA
authors.lastname: Deprest
mesh_terms: D000403:Airway Resistance / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D017097:Electric Impedance; D006548:Hernia, Diaphragmatic / Q000628:therapy*; D008168:Lung / Q000503:physiopathology*; D011817:Rabbits; D015656:Respiratory Mechanics / Q000502:physiology*