Is microbiological analysis of donor cornea transport culture media necessary?
OBJECTIVE
To investigate microbial contamination of the transport medium. Examination of corneoscleral rims is not included in this series.


METHODS
Transport media of 63 consecutive grafts done at Tennent Institute of Ophthalmology, Glasgow, were collected for microbial examination.


RESULTS
None of the culture plates showed any growth after prolonged culture, and microscopy was negative in 100% of cases.


CONCLUSIONS
Routine culture of transport media may not be necessary.
Cornish
K S
KS
Tennent Institute of Ophthalmology, Gartnavel General Hospital, Glasgow, UK. kurtsc01@yahoo.co.uk
Ramamurthi
S
S
Butcher
I
I
Ramaesh
K
K
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003315:Cornea / Q000382:microbiology*; D016039:Corneal Transplantation; D003320:Corneal Ulcer / Q000517:prevention & control; D015925:Cryopreservation; D003470:Culture Media; D015818:Eye Infections, Bacterial / Q000517:prevention & control; D006801:Humans; D009926:Organ Preservation; D012189:Retrospective Studies; D012590:Sclera / Q000382:microbiology*; D014019:Tissue Donors
D003470:Culture Media