title: Design of fluorescent assays for cyanide and hydrogen peroxide based on the inner filter effect of metal nanoparticles.
abstract: We have demonstrated the design of a new type fluorescent assay based on the inner filter effect (IFE) of metal nanoparticles (NPs), which is conceptually different from the previously reported metal NPs-based fluorescent assays. With a high extinction coefficient and tunable plasmon absorption feature, metal NPs are expected to be capable of functioning as a powerful absorber to tune the emission of the fluorophore in the IFE-based fluorescent assays. In this work, we presented two proof-of-concept examples based on the IFE of Au NPs by choosing MDMO-PPV as a model fluorophore, whose fluorescence could be tuned by the absorbance of Au NPs with a much higher sensitivity than the corresponding absorbance approach. While the first assay worked in a turn-on mode upon the etching of Au NPs by the analyte, CN(-), the second one functioned in a turn-off mode upon the catalytic growth of Au NPs by the analyte, H(2)O(2). As a result, the present IFE-based approach can detect cyanide ranging from 1.0 x 10(-6) to 6.0 x 10(-4) M with a detection limit of 6.0 x 10(-7) M and H(2)O(2) ranging from 1.5 x 10(-7) to 2.2 x 10(-5) M with a detection limit of 8.5 x 10(-8) M, respectively. Notably, the present IFE-based approach allows the design of fluorescent assays in a more simple, time-saving, and economical approach when compared with conventional metal NPs-based fluorescent assays, since no modification step of the fluorophore was needed any more.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Changchun, 130022, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
mesh_terms: D002384:Catalysis; D003486:Cyanides / Q000032:analysis*; D005374:Filtration; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D003486:Cyanides; D011145:Polyvinyls; D006046:Gold; D006861:Hydrogen Peroxide