Qualitative and quantitative proteomic profiling of cripto(-/-) embryonic stem cells by means of accurate mass LC-MS analysis.
Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto(-/-) murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto(-/-) ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene.
Chambery
Angela
A
Dipartimento di Scienze della Vita, Seconda Universita di Napoli, I-81100 Caserta, Italy. angela.chambery@unina2.it
Vissers
Johannes P C
JP
Langridge
James I
JI
Lonardo
Enza
E
Minchiotti
Gabriella
G
Ruvo
Menotti
M
Parente
Augusto
A
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods*; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000502:physiology; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis; D015203:Reproducibility of Results
D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D020543:Proteome; C489450:Tdgf1 protein, mouse; D004815:Epidermal Growth Factor