[The effect of bone marrow mesenchymal stem cell transplantation on diabetic cardiomyopathy].
OBJECTIVE
Diabetic cardiomyopathy (DCM), an important cause of heart failure, is characterized by microvascular pathologies and interstitial fibrosis. Bone marrow mesenchymal stem cells (MSCs) are pluripotent, which can differentiate into cardiomyocytes and vascular endothelial cells. They also secrete angiogenic and antiapoptotic factors. However, little information is available about the effect of MSCs transplantation on diabetic heart.


METHODS
MSCs were isolated from bone marrow of isogenic adult rats and cultured ex vivo. Eight weeks post streptozotocin injection, saline or exogenous MSCs labelled with 4'6-Diamidino-2-Phenylindole (DAPI) were injected into the femoral vein of diabetic rats and examined 4 weeks later by echocardiography, histopathologic analysis, reverse transcription polymerase chain reaction analysis for matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1, zymography analysis for activities of MMP-2 and Western blot analysis for troponin T.


RESULTS
Left ventricular posterior wall thickness and myocardial arteriolar density as well as the TIMP-1 mRNA and MMP-2 activity were significantly decreased in DCM group (P < 0.01 versus control group respectively), these changes were significantly attenuated by MSCs transplantation (P < 0.05 versus DCM). MSCs transplantation also significantly reduced fibrosis and downregulated MMP-9 mRNA in diabetic myocardium.


CONCLUSIONS
Intravenous MSCs transplantation could attenuate LV remodeling in DCM rats.
ZHANG
Nan
N
Department of Endocrinology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.
LI
Jia-hui
JH
WANG
Jian-an
JA
ZHANG
Hong-kun
HK
D000818:Animals; D016026:Bone Marrow Transplantation; D009202:Cardiomyopathies / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D020107:Troponin T / Q000096:biosynthesis; D020257:Ventricular Remodeling
D019715:Tissue Inhibitor of Metalloproteinase-1; D020107:Troponin T; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9