title: Analysis of a band 7/MEC-2 family gene of Porphyromonas gingivalis.
abstract: In vivo-induced antigen technology has previously been used to identify 115 genes induced in Porphyromonas gingivalis W83 during human infection. The aim of this study was to determine if one of these genes, PG1334, was important for the virulence of P. gingivalis. Analysis of plaque samples from persons with periodontitis revealed that PG1334 was expressed in 88.0% of diseased sites, compared with 42.1% of healthy sites, even though P. gingivalis was detected in equal numbers from both sites. A mutant of PG1334 was found to adhere to and to invade better than the parent strain, but did not persist as well in human coronary artery endothelial cells. Additionally, the mutant did not persist as well in a mouse abscess model. This gene appears to be important for the virulence of P. gingivalis, both in vivo and in vitro.
authors.affiliation: Department of Oral Biology, College of Dentistry and Center for Molecular Microbiology, University of Florida, Gainesville, FL 32610-0424, USA.
authors.forename: S
authors.initials: S
authors.lastname: Walters
authors.forename: P
authors.initials: P
authors.lastname: Rodrigues
authors.forename: M
authors.initials: M
authors.lastname: Bélanger
authors.forename: J
authors.initials: J
authors.lastname: Whitlock
authors.forename: A
authors.initials: A
authors.lastname: Progulske-Fox
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D016866:Bacteroidaceae Infections / Q000382:microbiology; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000382:microbiology; D004730:Endothelium, Vascular / Q000382:microbiology; D006801:Humans; D008100:Liver Abscess / Q000382:microbiology; D051379:Mice; D009154:Mutation / Q000235:genetics; D009504:Neutrophils / Q000502:physiology; D009875:Operator Regions, Genetic / Q000235:genetics; D009876:Operon / Q000235:genetics; D010518:Periodontitis / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins