In vitro evaluation of porous poly(L-lactic acid) scaffold reinforced by chitin fibers.
In this study, the previously reported porous three-dimensional poly(L-lactic acid) (PLLA) scaffolds reinforced by the chitin fibers (PLLA/CF) with and without the link were evaluated in vitro. Firstly, pH value of the phosphate buffered saline lixiviums of the PLLA/CF with different content of the chitin fibers was measured to get an appropriate content of the chitin fibers in the PLLA/CF. Then, the cell functions (attachment, proliferation, alkaline phosphatase per unit cell, total protein per unit cell, and osteonectin, osteopontin, and osteocalcin gene expression) of human osteoblast-like cells (SaOS2) cultured on the PLLA/CF with the link, PLLA/CF without the link and PLLA scaffold were compared. The results showed that the link treatment did not significantly influence the pH value of the lixiviums of the scaffolds, 30% volume content might be an appropriate content of the chitin fibers in PLLA/CF scaffold to keep the pH value of the lixiviums of the scaffolds between 7.0 and 7.2 during the lixiviation time of 16 weeks, the PLLA/CF scaffold was significantly better for the attachment, proliferation, differentiation, and mineralization of the osteoblast than PLLA, the link treatment did not significantly influence these cells activities, which further suggested that PLLA/CF with the link treatment might be an appropriate scaffold for tissue engineering.
Li
Xiaoming
X
Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan. x.m.li@hotmail.com
Liu
Xinhui
X
Dong
Wei
W
Feng
Qingling
Q
Cui
Fuzhai
F
Uo
Motohiro
M
Akasaka
Tsukasa
T
Watari
Fumio
F
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured / Q000166:cytology; D002686:Chitin / Q000737:chemistry*; D005786:Gene Expression Regulation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D015676:Osteonectin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D015676:Osteonectin; D010710:Phosphates; D011091:Polyesters; D011108:Polymers; D015675:Osteocalcin; D053495:Osteopontin; D002686:Chitin; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase