title: Image analysis software based on color segmentation for characterization of viability and physiological activity of biofilms.
abstract: The novel image analysis software package bioImage_L was tested to calculate biofilm structural parameters in oral biofilms stained with dual-channel fluorescent markers. By identifying color tonalities in situ, the software independently processed the color subpopulations and characterized the viability and metabolic activity of biofilms.
authors.affiliation: Department of Oral Biology, Faculty of Odontology, Malmö University, Malmö 20506, Sweden. luis.chavez.de.paz@od.mah.se
authors.forename: Luis E
authors.initials: LE
authors.lastname: Chávez de Paz
mesh_terms: D018441:Biofilms; D003116:Color; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D050296:Microbial Viability; D009055:Mouth / Q000382:microbiology; D012984:Software