title: Morphology and small subunit rDNA gene sequence of Pseudoamphisiella quadrinucleata n. sp. (Ciliophora, Urostylida) from the South China Sea.
abstract: The urosylid genus Pseudoamphisiella was established by Song (1996) with hitherto only two known congeners. In the present work, the morphology and infraciliature of a new member, Pseudoamphisiella quadrinucleata n. sp., a form with conspicuous alveolar layer and four macronuclear nodules isolated from the coastal waters both near Hong Kong and near Guangzhou, South China were investigated using living observation and protargol silver impregnation methods. Pseudoamphisiella quadrinucleata differs from other two known forms mainly by the number of macronuclear nodules: constantly four vs. two in Pseudoamphisiella alveolata and 24-57 in Pseudoamphisiella lacazei. To support this, the sequence of the small subunit rDNA of P. quadrinucleata n. sp. showed 14 and 74 nucleotides in comparison with that of the two known congeners, respectively, which hence firmly supports the validity of the new species.
authors.affiliation: Laboratory of Protozoology, College of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Shen
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Lin
authors.forename: Hongan
authors.initials: H
authors.lastname: Long
authors.forename: Miao
authors.initials: M
authors.lastname: Miao
authors.forename: Hongbin
authors.initials: H
authors.lastname: Liu
authors.forename: Khaled A S
authors.initials: KA
authors.lastname: Al-Rasheid
authors.forename: Weibo
authors.initials: W
authors.lastname: Song
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D016798:Ciliophora / Q000145:classification / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D048648:Macronucleus / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D054679:Ribosome Subunits, Small / Q000235:genetics*; D012623:Seawater / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal