title: Morphology of the bone marrow, spleen and liver during hematopoietic cell mobilization with cyclophosphamide in mice.
abstract: Cyclophosphamide (CY), the agent with cytoreductive activity, is widely exploited in cancer chemotherapy, and can be used alone or in combination with various cytokines and growth factors to stimulate the egress of hematopoietic stem/progenitor cells (HSPC) from the BM compartment. The aim of the present study was to exam the morphology and ultrastructure of the bone marrow, spleen and liver of mice injected intraperitoneally with a single dose of cyclophosphamide (200 mg/kg bw) and the localization of cells expressing markers of early hematopoietic cells in studied organs and the peripheral blood. We observed that the CY-induced morphological changes in the BM and spleen were reconstructed on day 4. of experiment, and the spleen was repopulated by HSPC on the 6th day. In this time, the highest number of c-Kit-R-positive cells was determined by flow cytometry in the peripheral blood. The results confirmed, that the egress of HSPC from the bone marrow into the peripheral blood was delayed compared to mice treated with G-CSF or GCS-F plus CY.
authors.affiliation: Departament of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Barcew
authors.forename: Anna
authors.initials: A
authors.lastname: Karbicka
authors.forename: Paweł
authors.initials: P
authors.lastname: Szumilas
authors.forename: Mariola
authors.initials: M
authors.lastname: Marchlewicz
authors.forename: Ryszard
authors.initials: R
authors.lastname: Grzegrzółka
authors.forename: Barbara
authors.initials: B
authors.lastname: Wiszniewska
authors.forename: Bogusław
authors.initials: B
authors.lastname: Machaliński
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects* / Q000648:ultrastructure; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D048430:Cell Shape; D003520:Cyclophosphamide / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D008099:Liver / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D012155:Reticulin / Q000187:drug effects; D013154:Spleen / Q000166:cytology* / Q000187:drug effects*; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D012155:Reticulin; D018800:Thy-1 Antigens; D003520:Cyclophosphamide; D019009:Proto-Oncogene Proteins c-kit