title: Osteogenic activity of constituents from Butea monosperma.
abstract: Phytochemical investigation from the stem bark of Butea monosperma, led to the isolation and identification of three new compounds named buteaspermin A (1), buteaspermin B (2) and buteaspermanol (3), along with 19 known compounds. The structure of compounds 1-22 were established on the basis of their spectroscopic data. The isolated compounds 2-17 were evaluated using neonatal (1-3 day old) rat calvaria derived primary osteoblast cultures. Five of these compounds 7, 10-13 showed promising osteogenic activity, attributed to increased osteoblast proliferation, differentiation and mineralization as evidenced by marked increase in expression of alkaline phosphatase, an early phase differentiation marker, and alizarin Red S staining of osteoblasts cultured for 48 h and von Kossa silver staining of nodules formed 15 days after culture with these compounds. Quantification of mineralization by optical density measurement of Alizarin Red S extracted from stained osteoblasts cultured for 7 days in presence of these compounds showed significant (P<0.05, vs corresponding vehicle control group) increase in mineralization. On the basis of biological results, structure-activity relationships are discussed.
authors.affiliation: Medicinal and Process Chemistry Division, Central Drug Research Institute, Chattar MAnzil Palace, M. G. Marg, Lucknow 226 001, Uttar Pradesh, India. mauryarakesh@rediffmail.com
authors.forename: Rakesh
authors.initials: R
authors.lastname: Maurya
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Yadav
authors.forename: Geetu
authors.initials: G
authors.lastname: Singh
authors.forename: Biju
authors.initials: B
authors.lastname: Bhargavan
authors.forename: P S
authors.initials: PS
authors.lastname: Narayana Murthy
authors.forename: Mahendra
authors.initials: M
authors.lastname: Sahai
authors.forename: Man Mohan
authors.initials: MM
authors.lastname: Singh
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005419:Flavonoids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D024301:Plant Bark / Q000378:metabolism*; D051381:Rats; D014197:Trees / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; D005419:Flavonoids; C004468:Alizarin Red S; D000469:Alkaline Phosphatase; D019342:Acetic Acid