title: [Jak/STAT signaling pathway of IL-11 in the protection of intestinal epithelial cells from neutron radiation].
abstract: OBJECTIVE
  To explore the effect of IL-11 on the activation of Jak/STAT pathway and the expressions of Bax and Bcl-2 in the intestinal epithelial cells exposed to neutron radiation.
  
  
  METHODS
  The BALB/c mice and IEC-6, irradiated by 4 Gy neutron with or without IL-11 treatment, served as in vivo and in vitro model seperately. The changes of the intestines, activity of Jak1 and STAT3 and expressions of Bax and Bcl-2 were observed by HE staining, Western blot, EMSA, immunohistochemistry and image analysis.
  
  
  RESULTS
  (1)Mice exposed to neutron radiation showed severe intestinal damages and no obvious regeneration was seen. IL-11-treated mice had a larger number of cryptal epithelial cells and crypts. (2)Neutron radiation decreased the activities of Jak1 and STAT3, while IL-11 increased their activities. (3) Neutron radiation decreased the expression of Bax and didn't change the level of Bcl-2 in the murine intestine. IL-11 administration decreased the expression of Bax and increased that of Bcl-2.
  
  
  CONCLUSIONS
  The mechanism of the intestinal protection of IL-11 in neutron irradiation might be that IL-11 stimulation triggered activation of Jak/STAT pathway, downregulated the expression of Bax and upregulated the expression of Bcl-2.
authors.affiliation: Department of Experimental Pathology, Institute of Radiation Medicine, AMMS, Beijing, China. wangrjok@163.com
authors.forename: Rui-Juan
authors.initials: RJ
authors.lastname: Wang
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Gong-Min
authors.initials: GM
authors.lastname: Chang
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Xu
authors.forename: Kai-Fei
authors.initials: KF
authors.lastname: Fu
authors.forename: Qing-Liang
authors.initials: QL
authors.lastname: Luo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D007150:Immunohistochemistry; D017370:Interleukin-11 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology*; D053613:Janus Kinase 1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009502:Neutrons / Q000009:adverse effects*; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects
chemical_list: D017370:Interleukin-11; D050796:STAT3 Transcription Factor; D053613:Janus Kinase 1