title: In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.
abstract: Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
authors.affiliation: Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Boston, Massachusetts 02114, USA.
authors.forename: Jean Y
authors.initials: JY
authors.lastname: Perentes
authors.forename: Trevor D
authors.initials: TD
authors.lastname: McKee
authors.forename: Carsten D
authors.initials: CD
authors.lastname: Ley
authors.forename: Hannah
authors.initials: H
authors.lastname: Mathiew
authors.forename: Michelle
authors.initials: M
authors.lastname: Dawson
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Padera
authors.forename: Lance L
authors.initials: LL
authors.lastname: Munn
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
authors.forename: Yves
authors.initials: Y
authors.lastname: Boucher
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology*; D005347:Fibroblasts; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D039641:Integrin beta Chains / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods*; D012065:Relaxin / Q000494:pharmacology; D012983:Soft Tissue Neoplasms / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D039641:Integrin beta Chains; D012065:Relaxin; D003094:Collagen