Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei.
Synchronisation of the Trypanosoma brucei cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 T. brucei cells growing in vitro with 10 microg ml(-1) hydroxyurea for 6h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form T. brucei.
Forsythe
Glynn R
GR
Division of Infection & Immunity and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, Scotland, UK.
McCulloch
Richard
R
Hammarton
Tansy C
TC
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000254:growth & development*
D006131:Growth Inhibitors; D006918:Hydroxyurea