title: Deterministic mechanical model of T-killer cell polarization reproduces the wandering of aim between simultaneously engaged targets.
abstract: T-killer cells of the immune system eliminate virus-infected and tumorous cells through direct cell-cell interactions. Reorientation of the killing apparatus inside the T cell to the T-cell interface with the target cell ensures specificity of the immune response. The killing apparatus can also oscillate next to the cell-cell interface. When two target cells are engaged by the T cell simultaneously, the killing apparatus can oscillate between the two interface areas. This oscillation is one of the most striking examples of cell movements that give the microscopist an unmechanistic impression of the cell's fidgety indecision. We have constructed a three-dimensional, numerical biomechanical model of the molecular-motor-driven microtubule cytoskeleton that positions the killing apparatus. The model demonstrates that the cortical pulling mechanism is indeed capable of orienting the killing apparatus into the functional position under a range of conditions. The model also predicts experimentally testable limitations of this commonly hypothesized mechanism of T-cell polarization. After the reorientation, the numerical solution exhibits complex, multidirectional, multiperiodic, and sustained oscillations in the absence of any external guidance or stochasticity. These computational results demonstrate that the strikingly animate wandering of aim in T-killer cells has a purely mechanical and deterministic explanation.
authors.affiliation: Department of Computational Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America.
authors.forename: Mun Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Maly
mesh_terms: D000818:Animals; D002451:Cell Compartmentation / Q000276:immunology; D016764:Cell Polarity / Q000276:immunology*; D018385:Centrosome / Q000276:immunology / Q000378:metabolism; D003595:Cytoplasmic Streaming / Q000276:immunology; D006801:Humans; D007365:Intercellular Junctions / Q000276:immunology; D008213:Lymphocyte Activation / Q000502:physiology*; D008870:Microtubules / Q000276:immunology; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000276:immunology; D055611:Natural Killer T-Cells / Q000276:immunology / Q000378:metabolism*
chemical_list: D020409:Molecular Motor Proteins