Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS.
Brevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin-R (TN-R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN-R, and concomitantly phosphacan, from an axonal diameter-dependent association with nodes to an axonal diameter independent association. Supported by the co-immunoprecipitation results, these observations indicate that the presence of TN-R and phosphacan at nodes is normally brevican-dependent, while in the absence of brevican these molecules can also be recruited by versican V2. The versican V2 and Bral1 distribution was not affected, thus indicating a brevican-independent role of these two molecules for establishing hyaluronan-binding matrices at the nodes. Our results revealed that brevican plays a crucial role in determining the specialization of the hyaluronan-binding nodal matrix assemblies in large diameter nodes.
Bekku
Yoko
Y
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
Rauch
Uwe
U
Ninomiya
Yoshifumi
Y
Oohashi
Toshitaka
T
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism / Q000502:physiology; D058581:Brevican; D002490:Central Nervous System / Q000033:anatomy & histology*; D011508:Chondroitin Sulfate Proteoglycans / Q000172:deficiency / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D006820:Hyaluronic Acid / Q000378:metabolism; D047468:Immunoprecipitation / Q000379:methods; D037181:Lectins, C-Type / Q000172:deficiency / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000502:physiology*; D011901:Ranvier's Nodes / Q000378:metabolism*; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5 / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism
C553057:Bcan protein, mouse; D058581:Brevican; D011508:Chondroitin Sulfate Proteoglycans; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D019063:Tenascin; C074638:tenascin R; D006820:Hyaluronic Acid; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5