title: Low dose estrogen supplementation reduces mortality of mice in estrogen-dependent human tumor xenograft model.
abstract: Breast cancer is one of the most frequent female cancers in the Western world. Perturbation of estrogen levels by hormone replacement therapy or pregnancy is associated with a variety of diseases, including breast cancer. Estrogen supplementation is required to establish appropriate animal models for estrogen-related diseases. In this report, we demonstrated that supplementation with high doses of 17beta-estradiol results in deaths in estrogen-dependent MCF-7 tumor xenograft model. Renal damage and bladder stone formation was implicated as a major cause of death. The mortality rate was significantly reduced when mice received a low dose of 17beta-estradiol. We also confirmed that low dose of 17beta-estradiol supplementation can support the growth of tumors in MCF-7 tumor xenograft model. These results suggest that low dose estrogen supplementation may be more appropriate in estrogen-dependent tumor xenograft models.
authors.affiliation: Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, Republic of Korea.
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Moo Rim
authors.initials: MR
authors.lastname: Kang
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
authors.forename: Won Kee
authors.initials: WK
authors.lastname: Yoon
authors.forename: Jang Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Taek Chang
authors.initials: TC
authors.lastname: Lee
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000230:Adenocarcinoma / Q000401:mortality* / Q000473:pathology / Q000628:therapy*; D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage*; D004967:Estrogens / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009376:Neoplasms, Hormone-Dependent / Q000401:mortality* / Q000473:pathology / Q000628:therapy*; D010052:Ovariectomy; D016019:Survival Analysis; D013997:Time Factors; D001744:Urinary Bladder Calculi / Q000139:chemically induced / Q000473:pathology
chemical_list: D004967:Estrogens; D004958:Estradiol