In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
Perentes
Jean Y
JY
Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, 100 Blossom Street, Boston, Massachusetts 02114, USA.
McKee
Trevor D
TD
Ley
Carsten D
CD
Mathiew
Hannah
H
Dawson
Michelle
M
Padera
Timothy P
TP
Munn
Lance L
LL
Jain
Rakesh K
RK
Boucher
Yves
Y
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology*; D005347:Fibroblasts; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D039641:Integrin beta Chains / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods*; D012065:Relaxin / Q000494:pharmacology; D012983:Soft Tissue Neoplasms / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
D039641:Integrin beta Chains; D012065:Relaxin; D003094:Collagen