title: Detection of culturable and nonculturable Legionella species from hot water systems of public buildings in Japan.
abstract: OBJECTIVE
  To investigate the prevalence of culturable and nonculturable Legionella species in hot water systems of public buildings in Japan and assess the risk factors associated with Legionella contamination in hot water systems.
  
  
  RESULTS
  Legionella species were detected by conventional culture and molecular methods in 130 water samples collected from 40 buildings. A total of 26 (20.0%) water samples from 17 (42.5%) buildings were positive by culture, qualitative PCR or both methods: Legionella pneumophila and Leg. anisa were detected in four samples by a culture method, whereas 23 samples were positive by qualitative PCR, with the presence of various Legionella species confirmed by sequencing. Of these 23 samples, bacterial counts were quantifiable in 21 by real-time PCR (from 1.7 x 10(5) to 2.6 x 10(11) cells per litre). Phylogenetic analysis of amplified partial 16S rRNA gene showed close relations to various species of Legionella, including Leg. anisa and Leg. micdadei, all of which have been associated with respiratory diseases or increased antibody titres in human sera. Assessment of risk factors showed that turbidity, free chlorine concentration, iron concentration and heterotrophic plate count (HPC) were significantly associated with Legionella contamination (P < 0.05).
  
  
  CONCLUSIONS
  Contamination of hot water systems of public buildings with culturable and nonculturable Legionella species may be a potential risk factor for Legionella infection in Japan. Adequate levels of chlorine, low levels of iron and HPC are important maintenance measures in the reduction of Legionella contamination in hot water systems.
  
  
  CONCLUSIONS
  More than 40% of hot water systems in the Japanese public buildings examined were contaminated by not only culturable Leg. pneumophila and Leg. anisa but also by nonculturable pathogenic species. To our knowledge, this is the first report of both culturable and nonculturable Legionella contamination in hot water systems of public buildings in Japan.
authors.affiliation: Department of Environment and Water, Osaka Prefectural Institute of Public Health, Higashinari-ku, Osaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Edagawa
authors.forename: A
authors.initials: A
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Doi
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Tomioka
authors.forename: K
authors.initials: K
authors.lastname: Sakabe
authors.forename: C
authors.initials: C
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006358:Hot Temperature; D007564:Japan; D007875:Legionella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011633:Public Facilities / Q000592:standards; D012336:RNA, Ribosomal, 16S; D012307:Risk Factors; D014876:Water Pollution; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S