title: [Investigation of plasmid-mediated AmpC beta-lactamase types in Klebsiella spp. and Escherichia coli isolates resistant or intermediate to cefoxitin].
abstract: Plasmid mediated AmpC beta-lactamases are reported from Enterobacteriaceae with increasing frequency. There have been reports of treatment failures in patients infected with these organisms and given broad-spectrum cephalosporins. The aim of this study was to investigate the presence of plasmid mediated AmpC beta-lactamases in Escherichia coli and Klebsiella spp. A total of 41 strains of cefoxitin resistant or intermediate E. coli (n= 27) and Klebsiella spp. (n= 14) were collected from january 2005 to January 2006 at Akdeniz University Hospital Central Laboratory. Three-dimensional test was used as a phenotypic confirmatory test. Analytical isoelectric focusing electrophoresis was used to measure the pl values of the beta-lactamases. Plasmid mediated AmpC enzyme genes were amplified using multiplex polymerase chain reaction and sequenced by Beckman Coulter CEQ 8000. AmpC beta-lactamases were only detected in two isolates (7.4%) of E. coli. These isolates produced CMY-2 like enzymes and have either CTX-M or TEM enzyme. Transferable AmpC beta-lactamases are associated with multiple antibiotic resistance. Therefore detection of these enzymes in gram-negative bacteria has a clinical importance, since it can often provide valuable information to clinicians leading to more effective and appropriate use of antimicrobials.
authors.affiliation: Akdeniz Universitesi Tip Fakültesi, Tibbi Mikrobiyoloji Anabilim Dali, Antalya.
authors.forename: Hadiye
authors.initials: H
authors.lastname: Demirbakan
authors.forename: Kenan
authors.initials: K
authors.lastname: Midilli
authors.forename: Dilara
authors.initials: D
authors.lastname: Oğünç
authors.forename: Nevgün
authors.initials: N
authors.lastname: Ozen
authors.forename: Gözde
authors.initials: G
authors.lastname: Ongüt
authors.forename: Duygu
authors.initials: D
authors.lastname: Dağlar
authors.forename: Derya
authors.initials: D
authors.lastname: Mutlu
authors.forename: Betil
authors.initials: B
authors.lastname: Ozhak
authors.forename: Dilek
authors.initials: D
authors.lastname: Colak
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002440:Cefoxitin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing; D007709:Klebsiella / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D011815:R Factors / Q000502:physiology*; D014421:Turkey; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002440:Cefoxitin; C048795:AmpC beta-lactamases; D001618:beta-Lactamases