[Dicer interacts with Wig-1 protein].
Dicer is a key dsRNase in RNA interference, and Wig-1 is a p53-induced zinc finger protein with dsRNA-binding activity. In this report, we demonstrated a direct interaction between Dicer and Wig-1. Dicer associated with Wig-1, which was expressed as a glutathione S-transferase fusion protein, in a dose-dependent manner by GST pull-down assay. In a reciprocal experiment, Wig-1 appeared to compete with GST-eIF2C1 for the binding of Dicer. Far-western analysis showed that Dicer could bind to GST-Wig-1 directly. Furthermore, it was found that Wig-1 could increase the dsRNase activity of Dicer in vitro. Our findings raise the possibility that Dicer and Wig-1 may play a common role in dsRNA-related gene regulation.
Huang
Jian
J
Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University, School of Medicine, Shanghai 200025. hj1970@hotmail.com
Wang
Hai Bo
HB
Yao
Ling
L
Zheng
Xin Min
XM
Radmark
Olof
O
D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins; D043244:Ribonuclease III
D002352:Carrier Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C436503:ZMAT3 protein, human; D004722:Endoribonucleases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases