Plug-based microfluidics with defined surface chemistry to miniaturize and control aggregation of amyloidogenic peptides.
Small with control: For miniaturization of protein aggregation experiments the interfacial chemistry must be controlled to avoid protein aggregation caused by interfacial adsorption. Plug-based microfluidics with defined surface chemistry (see schematic picture) can then be used to perform hundreds of aggregation experiments with volume-limited samples, such as cerebrospinal fluid from mice.
Meier
Matthias
M
Department of Chemistry, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
Kennedy-Darling
Julia
J
Choi
Se Hoon
SH
Norstrom
Eric M
EM
Sisodia
Sangram S
SS
Ismagilov
Rustem F
RF
D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D008904:Miniaturization; D010455:Peptides / Q000737:chemistry*; D018401:Sample Size; D013499:Surface Properties
D000682:Amyloid; D010455:Peptides