[Study on combined transplantation of rabbit's marrow stroma cells and hematomas in bone healing].
OBJECTIVE
To study the potentiality of osteanagenesis of the hematomas formed around the fractures and that of the marrow stroma cells, evaluate the effect of the combined trans-plantation of the hematoma and the marrow stroma cells, to explore a new method to accelerate the union of fracture.


METHODS
The bone defect models were made on the tibias of the New-Zealand's rabbits. The hematomas formed around the fracture were taken out 3 days latter after the operation, the marrow stroma cells were abstracted from the femoral marrow simultaneously. And then the mixture of the hematoma and the marrow stroma cells were transplanted to the defects of the tibias in the experiment group, and the hematoma transplanted simply to the same place in the control group. The radio-graph and the histological observation of the osteotylus were carried out regularly post-operation.


RESULTS
1) There was a significant difference in osteotylus quantity between the two groups: more osteotylus and obvious periosteal proliferation were found in the experiment group than that in the control group which accepted the transplantation of the hematomas alone. 2) There was a significant difference in osteoblast number between the two groups: more sclerotomal-like cells were observed under the microscope in the experiment group than that in the control group.


CONCLUSIONS
Marrow stroma cells have great potentiality of osteoanagenesis. The result of combined transplantation of the marrow stroma cells and the hematomas is more effective than that of simple transplantation of the bone hematoma.
Li
Gui-tao
GT
Department of Orthopaedics, Second People's Hospital of Guangdong Province, Guangzhou 510317, Guangdong, China. lgtgk@163.com
Peng
Zhan-xian
ZX
Zhang
Guo-hong
GH
Luo
Di-xin
DX
Chen
Wei-jian
WJ
Xu
Wang-yang
WY
Qi
Yong
Y
D000818:Animals; D001773:Blood Cells / Q000637:transplantation*; D016026:Bone Marrow Transplantation; D005260:Female; D017102:Fracture Healing; D006406:Hematoma / Q000601:surgery*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D011817:Rabbits; D011897:Random Allocation; D017154:Stromal Cells / Q000637:transplantation*; D013977:Tibia / Q000293:injuries / Q000503:physiopathology / Q000601:surgery; D013978:Tibial Fractures / Q000503:physiopathology / Q000601:surgery / Q000628:therapy*; D014182:Transplantation, Autologous