Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae.
Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.
Fujiwara
Takayuki
T
Research Information Center for Extremophile, Rikkyo University, 3-34-1 Nishiikebukuro, Toshima, Tokyo 171-8501, Japan.
Misumi
Osami
O
Tashiro
Kousuke
K
Yoshida
Yamato
Y
Nishida
Keiji
K
Yagisawa
Fumi
F
Imamura
Sousuke
S
Yoshida
Masaki
M
Mori
Toshiyuki
T
Tanaka
Kan
K
Kuroiwa
Haruko
H
Kuroiwa
Tsuneyoshi
T
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism*; D015870:Gene Expression; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D018087:Plastids / Q000378:metabolism; D000461:Rhodophyta / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
D020418:Algal Proteins