Expression of chemokines and adhesion molecules in human coronary artery endothelial cells infected with Chlamydia (Chlamydophila) pneumoniae.
Chlamydia pneumoniae has during recent years been associated with cardiovascular disease and atherosclerosis. Chemokines, leukocyte adhesion proteins and metalloproteinases are significant for chemotaxis and attachment of leukocytes to vessel walls, and for stability of atherosclerotic plaques. To determine the ability of C. pneumoniae to elicit inflammation in a relevant target host cell, we infected human coronary artery endothelial cells (HCAEC) with a clinical isolate of C. pneumoniae. Extracellular release of five chemokines, two adhesion proteins and a metalloproteinase was measured at different time points after infection using a cytometric bead assay and ELISA. Secretion of IL-8, MCP-1, MIG, IP-10 and ICAM-1 was significantly increased 48 h after C. pneumoniae infection of HCAEC in comparison with uninfected controls. Release of RANTES occurred already 6 h after infection. C. pneumoniae did not elicit release of E-selectin or MMP-1. We conclude that C. pneumoniae induces expression of proinflammatory components in HCAEC, which would promote migration of leukocytes towards endothelial cells. This suggests that C. pneumoniae initiates and propagates vascular inflammation in ways that contribute to coronary artery disease.
Högdahl
M
M
Department of Clinical Microbiology, University Hospital, Linköping, Sweden.
Söderlund
G
G
Kihlström
E
E
D050197:Atherosclerosis / Q000276:immunology* / Q000382:microbiology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis*; D002633:Chemotaxis; D002690:Chlamydia Infections / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D003331:Coronary Vessels / Q000276:immunology* / Q000382:microbiology; D004730:Endothelium, Vascular / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D013997:Time Factors
D015815:Cell Adhesion Molecules; D018925:Chemokines; D020781:Matrix Metalloproteinase 1