title: Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray.
abstract: In industrial process, yeast cells are exposed to ethanol stress that affects the cell growth and the productivity. Thus, investigating the intracellular state of yeast cells under high ethanol concentration is important. In this study, using DNA microarray analysis, we performed comprehensive expression profiling of two strains of Saccharomyces cerevisiae, i.e., the ethanol-adapted strain that shows active growth under the ethanol stress condition and its parental strain used as the control. By comparing the expression profiles of these two strains under the ethanol stress condition, we found that the genes related to ribosomal proteins were highly up-regulated in the ethanol-adapted strain. Further, genes related to ATP synthesis in mitochondria were suggested to be important for growth under ethanol stress. We expect that the results will provide a better understanding of ethanol tolerance of yeast.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Thai Nho
authors.initials: TN
authors.lastname: Dinh
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagahisa
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirasawa
authors.forename: Chikara
authors.initials: C
authors.lastname: Furusawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000431:Ethanol / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol