title: Improved left ventricular function after transplantation of microspheres and fibroblasts in a rat model of myocardial infarction.
abstract: As a novel and promising therapeutic strategy for heart failure, the application of different cell types is the subject of increasing research interest. In this study we investigated the effect of several cell types and microspheres (uniform polystyrene microspheres, 10 microm diameter) transplanted 4 weeks after induction of myocardial infarction in a rat model. Eight weeks after intramyocardial application of fibroblasts and microspheres, left ventricular function was significantly improved as demonstrated by isolated heart studies (Langendorff) and echocardiographic findings (LVDP fibroblasts 129 +/- 32.9 mmHg, LVDP microspheres 119.2 +/- 24.1 mmHg, fractional shortening (FS) microspheres 38.9 +/- 4.6%, FS fibroblasts 36.84 +/- 6.05%) in contrast to injection of macrophages or medium alone (LVDP medium 67 +/- 22.6 mmHg, LVDP macrophages 75.9 +/- 24.8 mmHg, FS macrophages 29.16 +/- 8.7%, FS medium 27.2 +/- 7.2%, P < 0.05). Signals of Bromodesoxy-Uridine (BrdU) labeled transplanted fibroblasts were detected in infarcted areas. Microspheres were recorded abundantly by autofluorescence. Significantly more apoptotic cells were observed in infarcted areas of macrophage (328.6 +/- 37.4 cells/mm(2)) and medium (338.7 +/- 16.5 cells/mm(2); P < 0.05) treated hearts compared to microsphere (233.2 +/- 16.8 cells/mm(2)) and fibroblast (232.2 +/- 19.1 cells/mm(2)) injected hearts. Neovascularization, as reflected by the density of CD 31 positive vessels in the infracted area, did not differ between the four groups studied. The increased number of macrophages in infarcted areas after fibroblast and microsphere injection (fibroblasts 94.7 +/- 7.1 cells/mm(2), microspheres 82.2 +/- 3.0 cells/mm(2), macrophages 56.02 +/- 9.93 cells/mm(2), medium 46.35 +/- 9.03 cells/mm(2), P < 0.05) suggests that the underlying mechanism of augmented left ventricular function might be based on inflammatory processes.
authors.affiliation: Medizinische Klinik I, Universitätsklinikum der RWTH Aachen, Pauwelsstrasse 30, 52057, Aachen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Schuh
authors.forename: Elisa A
authors.initials: EA
authors.lastname: Liehn
authors.forename: Alexander
authors.initials: A
authors.lastname: Sasse
authors.forename: Rebekka
authors.initials: R
authors.lastname: Schneider
authors.forename: Sabine
authors.initials: S
authors.lastname: Neuss
authors.forename: Christian
authors.initials: C
authors.lastname: Weber
authors.forename: Malte
authors.initials: M
authors.lastname: Kelm
authors.forename: Marc W
authors.initials: MW
authors.lastname: Merx
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D003326:Coronary Circulation / Q000502:physiology; D004195:Disease Models, Animal; D015150:Echocardiography, Doppler; D005260:Female; D005347:Fibroblasts / Q000637:transplantation*; D006801:Humans; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000637:transplantation; D008863:Microspheres; D009000:Monocytes / Q000276:immunology; D009203:Myocardial Infarction / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018919:Neovascularization, Physiologic / Q000502:physiology; D011137:Polystyrenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left / Q000502:physiology*
chemical_list: D011137:Polystyrenes