Laccase isoforms with unusual properties from the basidiomycete Steccherinum ochraceum strain 1833.
OBJECTIVE
To isolate and characterize the laccase isoforms from S. ochraceum 1833 - a new active producer of high extracellular laccase activity.


RESULTS
Three laccase isoforms (laccases I, II and III) with 57.5, 59.5 and 63 kDa molecular masses respectively were purified from S. ochraceum 1833 and in contrast to the known laccases had strongly pronounced absorption at 611 nm with molar extinction coefficients ranging from 7170 to 7830 mol(-1) l cm(-1). All isoforms showed maximal activity with ABTS at low pH (<or=2) and temperatures in the range 70-80 degrees C, were stable for long time of incubation at high temperature (60-80 degrees C) and at pH values ranging from 2 to 6. Laccase II showed a higher activity and wider substrate specificity. N-terminal amino acid sequence analysis of the purified laccase II (VQIGPVTDLH) showed 80% identity with the N-terminal amino acid sequence of laccase from Lentinula edodes [Appl Microbiol Biotechnol 60 (2002) 327].


CONCLUSIONS
Elevated temperature optima, high thermo- and pH-stabilities, the broad substrate specificity of the isoforms make the laccases from S. ochraceum 1833 a suitable model for biotechnological processes proceeding at high temperatures.


CONCLUSIONS
For the first time, new basidiomycete strain S. ochraceum was reported as a producer of novel thermostable, pH stable, acidophilic laccases with unusual spectral properties.
Chernykh
A
A
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS, Pushchino, Moscow Region, Russia.
Myasoedova
N
N
Kolomytseva
M
M
Ferraroni
M
M
Briganti
F
F
Scozzafava
A
A
Golovleva
L
L
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D020033:Protein Isoforms; D013053:Spectrophotometry; D013696:Temperature
D020033:Protein Isoforms; D042845:Laccase