title: The effect of preservation solutions UW and EC on purine concentration in rat kidney.
abstract: BACKGROUND
  Ischemia/reperfusion injury in organ transplantation is a multifactor process that may lead to organ damage and primary graft dysfunction. Perfusion is a process which creates a possibility of graft injury. Preservation solutions are thought to diminish the ischemic injury and an appropriate choice of the solution should guarantee a better graft function and good prognosis for graft survival. The aim of the study was to examine the effect of preservation solutions University of Wisconsin (UW) and Euro-Collins (EC) on purine concentration in rat kidney.
  
  
  METHODS
  The study was carried out on Wistar rat kidneys divided into 3 groups: kidneys perfused with 0.9% NaCl (control group), kidneys perfused with UW preservation solution, kidneys perfused with EC preservation solution. After bilateral nephrectomy the right kidney was immediately frozen in liquid nitrogen and the left kidney was placed in 4 degrees C UW, EC or 0.9% NaCl solution for 24 hours. The concentrations of purine nucleotides were determined using high-performance liquid chromatography (HPLC) method.
  
  
  RESULTS
  The concentrations ofpurine nucleotides in renal tissue without cold ischemia did not differ significantly between rats perfused with 0.9% NaCl, UW and EC solutions. The tissue concentrations of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), total adenine nucleotides (TAN), guanosine monophosphate (GMP) and inosine monophosphate (IMP) were significantly increased, whereas the concentrations of adenosine (Ado), inosine (Ino), guanosine (Guo), hypoxanthine (Hyp) and xanthine (Xan) were significantly lower in rats perfused with EC solution in comparison to rats perfused with 0.9% NaCl.
  
  
  CONCLUSIONS
  Purine concentration profile in rat kidney reflects a protective influence of EC and UW solutions on high-energy nucleotides in conditions when their depletion might be harmful to graft function.
authors.affiliation: Klinika Nefrologii, Transplantologii i Chorób Wewnetrznych Pomorskiej Akademii Medycznej w Szczecinie al. Powstańców Wlkp. 72, 70-111 Szczecin.
authors.forename: Leszek
authors.initials: L
authors.lastname: Domański
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Sulikowski
authors.forename: Maciej
authors.initials: M
authors.lastname: Romanowski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pawlik
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jakubowska
authors.forename: Violetta
authors.initials: V
authors.lastname: Dziedziejko
authors.forename: Magda
authors.initials: M
authors.lastname: Wiśniewska
authors.forename: Maciej
authors.initials: M
authors.lastname: Domański
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
authors.forename: Maria
authors.initials: M
authors.lastname: Olszewska
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ciechanowski
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006157:Guanosine Monophosphate / Q000378:metabolism; D007291:Inosine Monophosphate / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011685:Purine Nucleotides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019188:Organ Preservation Solutions; D011685:Purine Nucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D006157:Guanosine Monophosphate; D000255:Adenosine Triphosphate