Conformational transitions of immobilized DNA chains driven by pH with electrochemical output.
A pH-driven DNA sway rod is prepared by immobilizing thiolated DNA, mercaptohexanol, and cysteine on a gold electrode surface. As pH changes around the pI of cysteine, contrary electrostatic effect is produced between the negative DNA and amphoteric cysteine, which actuates reversible conformational transitions, such as sway of the DNA molecules, rodlike chain-to-globule, and so forth. The nanoscale motion can be detected by commonly used electrochemical technique and reversible electrochemical signal may be observed.
Meng
Fanben
F
Department of Biochemistry, Nanjing University, Nanjing 210093, People's Republic of China.
Liu
Yuexing
Y
Liu
Lei
L
Li
Genxi
G
D003545:Cysteine; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008968:Molecular Conformation; D013438:Sulfhydryl Compounds; D013499:Surface Properties
D004277:DNA, Single-Stranded; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; D003545:Cysteine