Can MTT be used to quantify the antioxidant activity of plant extracts?
Antioxidant properties of three different medicinal plant extracts from northern Nigeria were evaluated using the traditional qualitative 2,2, diphenyl-picrylhydrazyl (DPPH) method and a new 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) microdilution method described here. The results were in agreement. It is a simple, rapid and inexpensive method compared with many other methods for determining quantitative antioxidant activity of plant extracts.
Muraina
I A
IA
National Veterinary Research Institute, P.M.B. 01, Vom, Plateau State, Nigeria. muravet2002@yahoo.com
Suleiman
M M
MM
Eloff
J N
JN
D000975:Antioxidants / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue