title: In silico analysis of potential diagnostic targets from Burkholderia pseudomallei.
abstract: Administration of appropriate therapeutic regimes for infections arising from pathogenic species of Burkholderia is critically dependent upon rapid and accurate diagnoses. The purpose of this work is to establish a bioinformatic pipeline to assess protein sequences for their potential as diagnostic targets for the detection of Burkholderia species. Data are presented showing both a bioinformatic methodology for prediction of surface-associated and secreted proteins and its application to a test dataset of proteins from the pathogen B. pseudomallei. A subset of proteins, known to be produced by the organism, is identified which represents potential targets for development of new diagnostic reagents. In addition, a 'reverse diagnostics' bioinformatics approach has been established which can now be extended to whole genome analyses.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA.
authors.forename: Denis B
authors.initials: DB
authors.lastname: Thompson
authors.forename: Kerianne
authors.initials: K
authors.lastname: Crandall
authors.forename: Sarah V
authors.initials: SV
authors.lastname: Harding
authors.forename: Sophie J
authors.initials: SJ
authors.lastname: Smither
authors.forename: G Barrie
authors.initials: GB
authors.lastname: Kitto
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Brown
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis*; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000592:standards
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008565:Membrane Proteins; D011933:Reagent Kits, Diagnostic