title: Plasmacytoid dendritic cells prime alloreactive T cells to mediate graft-versus-host disease as antigen-presenting cells.
abstract: Dendritic cells (DCs) can be classified into 2 distinct subsets: conventional DCs (cDCs) and plasmacytoid DCs (pDCs). cDCs can prime antigen-specific T-cell immunity, whereas in vivo function of pDCs as antigen-presenting cells remains controversial. We evaluated the contribution of pDCs to allogeneic T-cell responses in vivo in mouse models of graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation by an add-back study of MHC-expressing pDCs into major histocompatibility complex-deficient mice that were resistant to GVHD. Alloantigen expression on pDCs alone was sufficient to prime alloreactive T cells and cause GVHD. An inflammatory environment created by host irradiation has the decisive role in maturing pDCs for T-cell priming but this process does not require Toll-like receptor signaling. Thus, functional outcomes of pDC-T-cell interactions depend on the immunologic context of encounter. To our knowledge, these results are the first to directly demonstrate an in vivo pathogenic role of pDCs as antigen-presenting cells in an antigen-specific T cell-mediated disease in the absence of other DC subsets and to provide important insight into developing strategies for tolerance induction in transplantation.
authors.affiliation: Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Science, Fukuoka, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Koyama
authors.forename: Daigo
authors.initials: D
authors.lastname: Hashimoto
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Aoyama
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Kennosuke
authors.initials: K
authors.lastname: Karube
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Niiro
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
authors.forename: Mitsune
authors.initials: M
authors.lastname: Tanimoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Akashi
authors.forename: Takanori
authors.initials: T
authors.lastname: Teshima
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000502:physiology*; D015551:Autoimmunity / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology / Q000502:physiology*; D005260:Female; D005802:Genes, MHC Class II; D006086:Graft vs Host Disease / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology