Vitrification of corneal endothelial cells in a monolayer.
An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.
Fan
Wen-Xia
WX
Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
Ma
Xue-Hu
XH
Liu
Tian-Qing
TQ
Cui
Zhan-Feng
ZF
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004728:Endothelium, Corneal / Q000166:cytology*; D019946:Propylene Glycol / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*
D003470:Culture Media; D019946:Propylene Glycol; D014199:Trehalose