title: The detection of possible sensor histidine kinases regulating citrate/malate metabolism from the bovine rumen microbial ecosystem.
abstract: OBJECTIVE
  To detect sensor histidine protein kinases (HPKs) similar to accessory gene regulator C (AgrC) from the rumen microbial ecosystem.
  Genes related to sensor HPKs were amplified by PCR using two pairs of agrC-specfic primers from DNA extracted from bovine rumen contents. The PCR products were cloned, sequenced and phylogenetically analysed. It appeared that two sequences were HPKs.
  
  
  CONCLUSIONS
  Although amino acid sequences deduced from the nucleotide sequences obtained in this study showed high similarities with sensor HPKs responding to citrate or C(4)-dicarboxylates, they did not show high similarities with AgrC.
  
  
  CONCLUSIONS
  This study revealed the presence in the rumen of sensor HPKs responding to citrate or C(4)-dicarboxylates, which could stimulate rumen fermentation. Therefore, it has been shown that citrate or C(4)-dicarboxylate metabolism is partially regulated by a two-component regulatory system in some rumen bacteria.
authors.affiliation: College of Animal Science, Northwest Agriculture and Forestry University, Yangling, Shaanxi, China.
authors.forename: W
authors.initials: W
authors.lastname: Sun
authors.forename: M
authors.initials: M
authors.lastname: Mitsumori
authors.forename: A
authors.initials: A
authors.lastname: Takenaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D002417:Cattle; D019343:Citric Acid / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D000071677:Histidine Kinase; D008293:Malates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011494:Protein Kinases / Q000235:genetics*; D012417:Rumen / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D008293:Malates; D019343:Citric Acid; C030298:malic acid; D011494:Protein Kinases; D000071677:Histidine Kinase