[Results of assessing the quality of Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum DNA by polymerase chain reaction in 2006-2007)].
Analyzing the results of detection of C. trachomatis, M. hominis, and U. urealyticum DNA by the participants of the Federal External Quality Assessment System in 2006-2007 indicated that the share of the fluorescent-probe amplification technique both in the real-time mode and by the end-point tended to increase. The above tendency coincided with the other--the higher proportion of laboratories detecting M. hominis and U. urealyticum at low concentrations (as low as 10(3) copies/ml) although no direct caused-and-effect relation could be established between these two tendencies. In 2006-2007, the section participants demonstrated a sufficiently high specificity (94-98%) in the detection of C. trachomatis, M. hominis, and U. urealyticum DNA while the sensitivity of studies left much to be desired--the proportion of correct results at the concentration of DNA of the above microorganisms of 10(3) copies/ml was as high as 73-82%. The considerable share of laboratories that fail to detect microorganisms at low concentrations suggests that it is necessary to continue work to enhance the sensitivity of this important type of laboratory studies.
Tvorogova
M G
MG
Gushchin
A E
AE
Malakhov
V N
VN
D001431:Bacteriological Techniques; D002692:Chlamydia trachomatis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D019535:Mycoplasma hominis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D016990:Ureaplasma urealyticum / Q000235:genetics*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic