The Chiptip: a novel tool for automated patch clamp.
To facilitate automated patch clamp measurements of ion channels in cells, the development of an all-glass Chiptip pipette is reported that may be combined with the previously described Flip-the-Tip technology. A single measurement requires less than 50 cells, and the addition of drugs for screening can be limited to very low volumes down to 1 microL. This apparatus is suitable for the study small cells, subcellular organelles and bacteria.
Lepple-Wienhues
Albrecht
A
Flyion GmbH, Waldhäuserstr. 64, D-72076 Tübingen, Germany. info@flyion.com
Lassen
Dirck
D
Hümmer
Alexander
A
Czubayko
Uwe
U
Knirsch
Martina
M
Golubovic
Andjelko
A
D000818:Animals; D001331:Automation; D001419:Bacteria / Q000187:drug effects; D002477:Cells / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D018408:Patch-Clamp Techniques / Q000295:instrumentation*