title: [Imbalance of T helper 1 cells/T helper 2 cells accelerated T-cell-mediated endothelium injury in patients with acute coronary syndromes].
abstract: OBJECTIVE
  To observe the T helper 1 and T helper 2 (Th1/Th2) balance and possible association to vascular endothelial cells injury in patients with acute coronary syndromes (ACS).
  
  
  METHODS
  Forty patients with ACS and 18 patients with stable angina pectoris (SAP) were included in this study. The concentrations of T helper 1/T helper 2 subsets related cytokines in plasma were evaluated by ELISA Kits. Cytotoxic activity of peripheral blood mononuclear cells (PBMCs) or PBMCs depleted CD(+) T cells against human umbilical vein endothelial cells (HUVECs) were evaluated by Cr51 cytotoxicity assay.
  
  
  RESULTS
  Concentrations of T helper 1 related cytokines IFN-gamma and IL-2 were significantly higher [IFN-gamma: (131.2 +/- 42.2) ng/L vs. (47.6 +/- 20.2) ng/L; IL-2: (83.7 +/- 21.3) ng/L vs. (46.2 +/- 16.7) ng/L, all P < 0.05] while T helper 2 related cytokine IL-10 concentration was significantly lower [(16.7 +/- 4.3) ng/L vs. (27.5 +/- 5.5) ng/L, P < 0.05] in patients with ACS compared to those in SAP patients. Cytotoxic activity of PBMCs against HUVECs in patients with ACS was also significantly higher than that in patients with SAP (28.84% +/- 4.20% vs. 20.28% +/- 2.71%, P < 0.05).
  
  
  CONCLUSIONS
  In patients with ACS, Th1 related cytokines were significantly upregulated while Th2 related cytokines were significantly downregulated. This imbalance of Th1/Th2 accelerated PBMCs mediated endothelium injury in patients with ACS.
authors.affiliation: Department of Cardiology, General Hospital of Jinan Military Area Command, Jinan 250031, China.
authors.forename: Shu-fang
authors.initials: SF
authors.lastname: HAN
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: LI
authors.forename: Ke-wei
authors.initials: KW
authors.lastname: LIU
authors.forename: Lin
authors.initials: L
authors.lastname: WANG
authors.forename: Rui
authors.initials: R
authors.lastname: CUI
authors.forename: Wei
authors.initials: W
authors.lastname: QIAN
authors.forename: Zhan-peng
authors.initials: ZP
authors.lastname: ZHUO
authors.forename: An-ji
authors.initials: AJ
authors.lastname: ZHANG
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood* / Q000276:immunology*; D000368:Aged; D000787:Angina Pectoris / Q000097:blood / Q000276:immunology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*
chemical_list: D007376:Interleukin-2; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma