title: DNA fingerprinting Candida species.
abstract: It is sometimes necessary to assess the genetic relatedness of isolates to identify the origin of an infection. In addition, evidence is accumulating that drug resistance can be associated with strains from a particular clade and that strains can exhibit anatomical specificity. It may, therefore, be valuable in the near future to screen for strains with a propensity for drug resistance. While a number of methods exist for genetically fingerprinting the infectious fungi, only a few provide the necessary resolution not only for distinguishing whether strains are highly related or unrelated, but also for grouping a strain in a particular clade. Here, we provide the procedures for performing the two methods that have proven most effective in the past 5 years: Southern blot hybridization of restriction fragments with complex probes and multilocus sequence typing (MLST).
authors.affiliation: Department of Biological Sciences, The University of Iowa, Iowa City, IA, USA.
authors.forename: Claude
authors.initials: C
authors.lastname: Pujol
authors.forename: David R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length