Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: evaluation by two molecular markers.
Analyses of MLEE, RAPD and LSSP-PCR were used to compare the panel of american tegumentary leishmaniasis (ATL) isolates obtained from lesions of patients with rare clinical manifestations of the disease and typical lesions. All of the 34 samples analyzed by MLEE demonstrated similar electromorphic profiles with Leishmania (Viannia) braziliensis reference strain. Through the RAPD analysis, nine genetic profiles (genotypes) were identified. LSSP-PCR corroborates the initial screening and phenetic analysis has grouped the isolates into two major clusters comprising the nine different genotypes. Prevalent genotype defined as LbmtDNAgen1 was detected in the largest number of isolates. There was no association between genotypes and clinical symptoms. However, two different genotypes could be identified in the initial (LbmtDNAGen9) and reactivated lesion (LbmtDNAGen3) of the same patient. Our results support the idea of a less pronounced genotypic diversity among L. (V.) braziliensis circulating in the State of Rio de Janeiro and demonstrate the useful application of these molecular markers in genetics variability studies.
Baptista
C
C
Centro de Referência em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, IPEC/Fiocruz, Av. Brasil, 4365, Rio de Janeiro, CEP 21040-900, Brazil. cibele.baptista@ipec.fiocruz.br
Schubach
A O
AO
Madeira
M F
MF
Leal
C A
CA
Pires
M Q
MQ
Oliveira
F S
FS
Conceição-Silva
F
F
Rosalino
C M V
CM
Salgueiro
M M
MM
Pacheco
R S
RS
D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000032:analysis; D004586:Electrophoresis / Q000379:methods; D004798:Enzymes / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007892:Leishmania braziliensis / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007897:Leishmaniasis, Mucocutaneous / Q000469:parasitology; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique
D016054:DNA, Protozoan; D004798:Enzymes; D005819:Genetic Markers