title: A novel real-time PCR method for KIR genotyping.
abstract: Genes encoding killer cell immunoglobulin-like receptors (KIRs) are variable among individuals. Sequence-specific primer-directed polymerase chain reaction (PCR) amplification (PCR-SSP) and sequence-specific oligonucleotide hybridization of the PCR-amplified products (PCR-SSO) are the methods currently used to characterize the diversity of KIR gene content. Both these methods include time-consuming post-PCR analyses. Here, we developed a real-time PCR method that identifies the presence or absence of 16 KIR genes during PCR and avoids post-PCR analyses. This method is specific, sensitive, shortens the turnaround time compared with the conventional PCR-SSP and PCR-SSO methods, and it can be easily adapted for automation.
authors.affiliation: Genetic Division, Genoa Biotechnology SA, São Paulo, Brazil.
authors.forename: L G T
authors.initials: LG
authors.lastname: Alves
authors.forename: R
authors.initials: R
authors.lastname: Rajalingam
authors.forename: F
authors.initials: F
authors.lastname: Canavez
mesh_terms: D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D054340:Receptors, KIR / Q000235:genetics*
chemical_list: D054340:Receptors, KIR