title: Glycomic approach for potential biomarkers on prostate cancer: profiling of N-linked glycans in human sera and pRNS cell lines.
abstract: Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.
authors.affiliation: Department of Chemistry, University of California Davis, Davis, CA, USA.
authors.forename: Maria Lorna A
authors.initials: ML
authors.lastname: de Leoz
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: An
authors.forename: Scott
authors.initials: S
authors.lastname: Kronewitter
authors.forename: Jaehan
authors.initials: J
authors.lastname: Kim
authors.forename: Sean
authors.initials: S
authors.lastname: Beecroft
authors.forename: Ruth
authors.initials: R
authors.lastname: Vinall
authors.forename: Suzanne
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Ralph
authors.initials: R
authors.lastname: de Vere White
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
authors.forename: Carlito
authors.initials: C
authors.lastname: Lebrilla
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism; D005583:Fourier Analysis; D020869:Gene Expression Profiling; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D011134:Polysaccharides; D017430:Prostate-Specific Antigen; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase