title: Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus.
abstract: Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. In the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.
authors.affiliation: Max-Planck-Institute for Terrestrial Microbiology, Karl-von Frisch-Strasse 1, 35043 Marburg, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Wagner
authors.forename: Silvia
authors.initials: S
authors.lastname: Berkner
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Ajon
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
authors.forename: Georg
authors.initials: G
authors.lastname: Lipps
authors.forename: Sonja-Verena
authors.initials: SV
authors.lastname: Albers
mesh_terms: D019847:Chromosomes, Archaeal / Q000235:genetics; D003434:Crossing Over, Genetic; D019641:DNA, Archaeal / Q000235:genetics; D017353:Gene Deletion; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D016944:Sulfolobus / Q000235:genetics*
chemical_list: D019641:DNA, Archaeal