Simple adhesive-tape-based sampling of tomato surfaces combined with rapid fluorescence in situ hybridization for Salmonella detection.
A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.
Bisha
Bledar
B
Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011, USA.
Brehm-Stecher
Byron F
BF
D017404:In Situ Hybridization, Fluorescence; D018551:Solanum lycopersicum / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*