title: [Study on optimum stimulation parameter for Shuigou (GV 26) in Xingnao Kaiqiao needling method].
abstract: OBJECTIVE
  to investigate the influence of acupuncture parameter on acupuncture effect and to probe the optimum stimulation parameter for Shuigou (GV 26) in Xingnao Kaiqiao needling method.
  
  
  METHODS
  The rat model of middle cerebral artery obstruction (MCAO) was replicated and "Shuigou" (GV 26) was stimulated by 9 acupuncture parameters combined by two factors at 3 levels, i. e. frequency 1, 2, 3 times/second, and needling duration 5 s, 60 s, 180 s. The effects of acupuncture at "Shuigou" (GV 26) with the 9 different acupuncture parameters on cerebral blood flow (CBF) in the MCAO rat were investigated by orthogonal test, factor analysis and one-way ANOVA.
  
  
  RESULTS
  Acupuncture effect was influenced by acupuncture frequency, acupuncture duration and their combination. Among them, the acupuncture duration was the most important factor. The best acupuncture effect could be attained by using as long acupuncture duration as possibly with the fastest acupuncture frequency.
  
  
  CONCLUSIONS
  In the Xingnao Kaiqiao needling method, acupuncture at "Shuigou" (GV 26) with fastest frequency and enough long duration can get the best acupuncture effect, which are the optimum stimulation parameters for "Shuigou" (GV 26).
authors.affiliation: Tianjin Acupuncture and Moxibustion Key Laboratory, Tianjin Acupuncture and Moxibustion Institute, Tianjin 300193, China. fanxiaonong@163.com
authors.forename: Xiao-nong
authors.initials: XN
authors.lastname: Fan
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Ya-jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Qian
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Zhang
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Wei
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Hui-qun
authors.initials: HQ
authors.lastname: Wu
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Shi
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D002539:Cerebral Arterial Diseases / Q000628:therapy*; D006801:Humans; D008297:Male; D009339:Needles; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar