title: Identification of marbling-related candidate genes in M. longissimus dorsi of high- and low marbled Hanwoo (Korean Native Cattle) steers.
abstract: This study was conducted to identify marbling-related candidate genes in M. longissimus dorsi of high- and low-marbled Hanwoo. The longissimus dorsi muscles were selected for gene expression from eight Hanwoo steer carcasses based on crude fat content. In the analysis of variance, gene expression of five candidate genes, FABP4, SCD, PPARgamma, Titin and Nebulin was determined to be significantly different between high- and low-marbled Hanwoo steers (P < 0.0001). The Pik-4 and CaMK II genes were also shown to have a significant effect on crude fat content (P < 0.01). In the analysis of the differential expression between high- and low marbled groups, FABP4 gene expression was approximately 2 times higher in the high marbled group relative to the low marbled group. However, the PPARgamma and SCD gene were highly expressed in the low marbled group. In addition, Titin and Nebulin were highly expressed in the low marbled group when placed under relatively high shear force. Finally, the Pik-4 and CaM K II gene also displayed a high expression pattern in the low marbled group.
authors.affiliation: National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Cho
authors.forename: Sang-Hong
authors.initials: SH
authors.lastname: Lee
authors.forename: Bum-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Nam-Kuk
authors.initials: NK
authors.lastname: Kim
authors.forename: Yeon-Ho
authors.initials: YH
authors.lastname: Choy
authors.forename: Kyoung-Hoon
authors.initials: KH
authors.lastname: Kim
authors.forename: Duhak
authors.initials: D
authors.lastname: Yoon
authors.forename: Seok-Ki
authors.initials: SK
authors.lastname: Im
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Oh
authors.forename: Eung-Woo
authors.initials: EW
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D000367:Age Factors; D000818:Animals; D050218:Body Fat Distribution; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics; D002417:Cattle / Q000033:anatomy & histology* / Q000235:genetics*; D064211:Connectin; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D007723:Korea; D008297:Male; D008460:Meat; D015778:Minor Histocompatibility Antigens; D009124:Muscle Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000033:anatomy & histology*; D047495:PPAR gamma / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013230:Stearoyl-CoA Desaturase / Q000235:genetics
chemical_list: D064211:Connectin; D050556:Fatty Acid-Binding Proteins; D015778:Minor Histocompatibility Antigens; D009124:Muscle Proteins; D047495:PPAR gamma; C035620:nebulin; D013230:Stearoyl-CoA Desaturase; D011494:Protein Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); C402182:phosphatidylinositol phosphate 4-kinase; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2