title: [Meiotic abnormalities of oocytes from patients with endometriosis submitted to ovarian stimulation].
abstract: OBJECTIVE
  to evaluate the meiotic spindle and the chromosome distribution of in vitro mature oocytes from stimulated cycles of infertile women with endometriosis, and with male and/or tubal infertility factors (Control Group), comparing the rates of in vitro maturation (IVM) between the two groups evaluated.
  
  
  METHODS
  fourteen patients with endometriosis and eight with male and/or tubal infertility factors, submitted to ovarian stimulation for intracytoplasmatic sperm injection have been prospectively and consecutively selected, and formed a Study and Control Group, respectively. Immature oocytes (46 and 22, respectively, from the Endometriosis and Control Groups) were submitted to IVM. Oocytes presenting extrusion of the first polar corpuscle were fixed and stained for microtubules and chromatin evaluation through immunofluorescence technique. Statistical analysis has been done by the Fisher's exact test, with statistical significance at p<0.05.
  
  
  RESULTS
  there was no significant difference in the IVM rates between the two groups evaluated (45.6 and 54.5% for the Endometriosis and Control Groups, respectively). The chromosome and meiotic spindle organization was observed in 18 and 11 oocytes from the Endometriosis and Control Groups, respectively. In the Endometriosis Group, eight oocytes (44.4%) presented themselves as normal metaphase II (MII), three (16.7%) as abnormal MII, five (27.8%) were in telophase stage I and two (11.1%) underwent parthenogenetic activation. In the Control Group, five oocytes (45.4%) presented themselves as normal MII, three (27.3%) as abnormal MII, one (9.1%) was in telophase stage I and two (18.2%) underwent parthenogenetic activation. There was no significant difference in meiotic anomaly rate between the oocytes in MII from both groups.
  
  
  CONCLUSIONS
  the present study data did not show significant differences in the IVM or in the meiotic anomalies rate between the IVM oocytes from stimulated cycles of patients with endometriosis, as compared with controls. Nevertheless, they have suggested a delay in the outcome of oocyte meiosis I from patients with endometriosis, shown by the higher proportion of oocytes in telophase I observed in this group.
authors.affiliation: Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: Ionara Diniz Evangelista Santos
authors.initials: ID
authors.lastname: Barcelos
authors.forename: Rodolpho Cruz
authors.initials: RC
authors.lastname: Vieira
authors.forename: Elisa Melo
authors.initials: EM
authors.lastname: Ferreira
authors.forename: Maria Cristina Picinato Medeiros de
authors.initials: MC
authors.lastname: Araújo
authors.forename: Wellington de Paula
authors.initials: Wde P
authors.lastname: Martins
authors.forename: Rui Alberto
authors.initials: RA
authors.lastname: Ferriani
authors.forename: Paula Andrea de Albuquerque Salles
authors.initials: PA
authors.lastname: Navarro
mesh_terms: D004715:Endometriosis / Q000235:genetics; D005260:Female; D005831:Genital Diseases, Female / Q000235:genetics; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000235:genetics; D008540:Meiosis; D008875:Middle Aged; D009865:Oocytes / Q000502:physiology; D010062:Ovulation Induction