title: Micro-CT for measuring marginal leakage of Class II resin composite restorations in primary molars prepared in vivo.
abstract: OBJECTIVE
  To evaluate the reliability of marginal leakage assessment of self-etch adhesive Class II resin composite restorations in primary molars prepared in vivo using the micro-CT.
  
  
  METHODS
  Fourteen primary molars, divided over seven ART and seven traditional resin composite Class II restorations, were subjected to marginal leakage test using a 50% (w/v) silver nitrate solution for 4 hours by a micro-CT. Two evaluators selected the image with the deepest marginal dye penetration for each restoration from the occlusal and approximal surfaces on consensus. The deepest dye penetration depth and total length of the axial cavity wall from both the occlusal and from the approximl surface were measured manually using morphometric quantification software by three evaluators independently. ANOVA was applied to test for differences in marginal leakage scores between the three evaluators.
  
  
  RESULTS
  There was no marginal leakage found in five traditional and one ART restoration. The percentage of silver nitrate penetration depth from the cervical-approximal site ranged from 10.2-92.6%. There was only a statistically significant difference observed in measuring total axial wall length from the cervical-approximal site between the three evaluators (P = 0.02). This explanatory investigation showed that the micro-CT was a very useful device for developing a standardized method for measuring marginal leakage from samples obtained in vivo. In combination with 50% (w/v) silver nitrate and 4-hour immersion, marginal leakage along the restoration-tooth tissue interface was accurately and reliably measured. (Am J Dent 2008;21:393-397).
authors.affiliation: Department of Pediatric Dentistry, Ege University, School of Dentistry, Izmir, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Eden
authors.forename: Asli
authors.initials: A
authors.lastname: Topaloglu-Ak
authors.forename: Vasli
authors.initials: V
authors.lastname: Topaloglu-Ak
authors.forename: Vincent
authors.initials: V
authors.lastname: Cuijpers
authors.forename: Jo E
authors.initials: JE
authors.lastname: Frencken
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002648:Child; D003188:Composite Resins / Q000737:chemistry*; D003737:Dental Cavity Preparation / Q000145:classification; D003743:Dental Enamel / Q000000981:diagnostic imaging; D003763:Dental Leakage / Q000000981:diagnostic imaging*; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent / Q000145:classification*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008422:Materials Testing; D008963:Molar / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D019279:Resin Cements / Q000737:chemistry; D016622:Silver Staining; D013499:Surface Properties; D013997:Time Factors; D014094:Tooth, Deciduous / Q000000981:diagnostic imaging*; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D003188:Composite Resins; D003764:Dental Materials; C079764:Pertac hybrid; C408584:Prompt L-Pop; D019279:Resin Cements; D017438:Bisphenol A-Glycidyl Methacrylate