title: Enhanced release of chitosanase from Streptomyces griseus through direct interaction of liposome with cell membrane under heat stress.
abstract: A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41 degrees C increased the chitosanase production and release to 2.2 times higher than that at 37 degrees C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41 degrees C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41 degrees C, clearly showing the importance of the direct interaction between membrane and membrane.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Kien Xuan
authors.initials: KX
authors.lastname: Ngo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Umakoshi
authors.forename: Toshinori
authors.initials: T
authors.lastname: Shimanouchi
authors.forename: Huong Thi
authors.initials: HT
authors.lastname: Bui
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Kuboi
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006358:Hot Temperature; D008081:Liposomes; D013305:Streptomyces griseus / Q000201:enzymology*
chemical_list: D008081:Liposomes; D006026:Glycoside Hydrolases; C019868:chitosanase