Effect of collagen oligopeptide injection on rabbit tenositis.
Effects of the collagen oligopeptide (COP) were examined by its repeat injection into the inflammatory rabbit Achilles tendon (Tendo calcaneus communis), in which tenositis was induced by injection of bacterial collagenase. COP was evaluated 5 times over a period of 3 weeks to 1 month after injection of collagenase. At 1 month after treatment, the therapeutic effect of COP was evaluated by examining the structure of collagen fibrils, amount and components of glycosaminoglycans (GAGs) and matrix metalloproteinases (MMPs), and compared with the saline injection, control, and normal groups. The Achilles tendon of rabbit in the control group (no COP injection) and saline injection group showed a notable increase in the number of fine collagen fibrils, a change in GAG composition and increase in the amount of pro-MMP-2, indicating the weakening of the tendon. In contrast, the size distribution of collagen fibrils, GAG composition and the amount of pro-MMP-2 was similar to those in the normal group. These results suggest that COP injection promotes healing processes of the tendon injury.
Ueda
Hiromi
H
Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Meguri
Natsuko
N
Minaguchi
Jun
J
Watanabe
Takafumi
T
Nagayasu
Aya
A
Hosaka
Yoshinao
Y
Tangkawattana
Prasarn
P
Kokai
Yasuo
Y
Takehana
Kazushige
K
D000125:Achilles Tendon / Q000473:pathology; D000818:Animals; D003094:Collagen / Q000008:administration & dosage / Q000627:therapeutic use*; D017364:Collagenases / Q000009:adverse effects; D004195:Disease Models, Animal; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D015552:Injections, Intralesional / Q000662:veterinary; D008297:Male; D010455:Peptides / Q000008:administration & dosage / Q000627:therapeutic use*; D011817:Rabbits; D052256:Tendinopathy / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
D006025:Glycosaminoglycans; D010455:Peptides; D003094:Collagen; D017364:Collagenases