A possible role of vimentin on the cell surface for the activation of latent transforming growth factor-beta.
Latent TGF-beta (LTGF-beta) has to be converted to active TGF-beta for its activities. Previously, we reported that certain fragments of latency associated peptide (LAP) augmented LTGF-beta activation via increase in binding of LTGF-beta to the endothelial cell (EC) surface followed by cell-associated proteolysis. By searching for EC membrane proteins crosslinked with the LAP fragment, we identified the molecule bound to LAP fragment as vimentin. Moreover, the LAP fragment-induced LTGF-beta activation was attenuated by anti-vimentin antibody. These results indicate that binding of the LAP fragment to vimentin on the cell surface is indispensable for LTGF-beta activation by the LAP fragment.
Nishida
Yasutake
Y
Innovative Drug Research Laboratories, Research Division, Kyowa Hakko Kirin Co. Ltd., Tokyo 194-8533, Japan.
Shibata
Kenji
K
Yamasaki
Motoo
M
Sato
Yasufumi
Y
Abe
Mayumi
M
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D053779:Latent TGF-beta Binding Proteins / Q000819:agonists* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
D053779:Latent TGF-beta Binding Proteins; D010455:Peptides; D016212:Transforming Growth Factor beta; D014746:Vimentin