[An antagonism between the telomeric and Polycomb-dependent chromatin at the end of terminally deleted Drosophila melanogaster chromosomes].
The results obtained at our laboratory during several last years suggest that a specific telomeric chromatin is formed at the terminal regions of Drosophila melanogaster DNA of 4-5 kb in length. In this work, we have studied how the telomeric chromatin influences the Polycomb-dependent repression. Many Polycomb proteins are involved in the formation of subtelomeric chromatin and inhibit the expression of a transgene inserted into it. However, the question on a positive or negative effect of telomeric chromatin on the assembly of subtelomeric protein complexes is yet open. Using a model system, in which a P element inserted upstream of the yellow gene promoter and located at the end of a terminally deleted chromosome can serve as a binding site for Pc-G proteins in the presence of php1 mutant allele, we have shown that a specific structure of the telomeric chromatin negatively influences the formation of Polycomb-dependent repression complex. These results suggest an antagonism between the telomeric and subtelomeric (Pc-G-dependent) chromatins.
Proskuriakov
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KA
Mel'nikova
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LS
D000818:Animals; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D063150:Polycomb Repressive Complex 1; D011401:Promoter Regions, Genetic / Q000235:genetics; D016615:Telomere / Q000235:genetics* / Q000378:metabolism
D002843:Chromatin; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; C066770:Pc protein, Drosophila; D063150:Polycomb Repressive Complex 1