Establishment of an in vitro transgene expression system in epimastigotes of Trypanosoma congolense.
Trypanosoma congolense epimastigote forms (EMFs) adhere to the tsetse fly proboscis, proliferate, and differentiate into animal-infective metacyclic forms (MCFs). This differentiation step, called metacyclogenesis, is indispensable for the cyclical transmission of the parasite. Although an in vitro metacyclogenesis culture system was established several decades ago, few genetic tools have been utilized to investigate the molecular mechanisms underlying T. congolense metacyclogenesis. This study established a transgene expression system using an in vitro derived EMF of T. congolense IL3000, and the transgenic EMF successfully underwent metacyclogenesis in vitro. The newly constructed expression vector pSAK was designed for integration into the alpha-beta tubulin locus, which is tandemly arranged in the T. congolense genome. The expression cassette of pSAK/enhanced green fluorescent protein (eGFP) was transfected into the EMF by electroporation. An EMF expressing eGFP was successfully generated and differentiated into an MCF that constitutively expressed eGFP. The in vitro metacyclogenesis system in combination with the transgenic EMF technique will be important tools to investigate the molecular mechanisms of metacyclogenesis.
Sakurai
Tatsuya
T
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Tanaka
Miho
M
Kawazu
Shin-Ichiro
S
Inoue
Noboru
N
D000818:Animals; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008018:Life Cycle Stages; D018613:Microscopy, Confocal; D010274:Parasitology / Q000379:methods; D014162:Transfection; D019076:Transgenes / Q000235:genetics / Q000502:physiology; D014348:Trypanosoma congolense / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins