Diamino acid derivatives of PpIX as potential photosensitizers for photodynamic therapy of squamous cell carcinoma and prostate cancer: in vitro studies.
Photodynamic therapy (PDT) is an alternative treatment modality involving light activated drugs, called photosensitizers (PSs), to treat cancer and non-cancerous conditions. The search for new compounds which might become effective PSs is the major direction for PDT development. In the present work we have studied the dark toxicity, intracellular localization and photodynamic properties of four potential, water soluble, second generation PSs--PP(Arg)(2), PP(Ser)(2)Arg(2), PP(Ala)(2)Arg(2), PP(Phe)(2)Arg(2), all diamino acid derivatives of protoporphyrin IX. Human prostate cancer (DU-145) and squamous carcinoma (A431) cells were used as experimental model. Among investigated compounds PP(Ser)(2)Arg(2) exhibited the lowest dark toxicity and the highest PDT effectiveness towards both cell lines. Fluorescence microscopy revealed the time-dependent changes in intracellular localization of the PS which were related to the phototoxicity. The results show that PP(Ser)(2)Arg(2) may be a potential PS for PDT.
Kwitniewski
Mateusz
M
Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdansk, ul. Do Studzienki 38, 80-227 Gdansk, Poland. kwitniewski@amg.gda.pl
Juzeniene
Asta
A
Ma
Li-Wei
LW
Glosnicka
Renata
R
Graczyk
Alfreda
A
Moan
Johan
J
D000599:Amino Acids, Diamino / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007700:Kinetics; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000493:pharmacokinetics; D011471:Prostatic Neoplasms / Q000628:therapy*; D011524:Protoporphyrins / Q000737:chemistry* / Q000493:pharmacokinetics
D000599:Amino Acids, Diamino; D017319:Photosensitizing Agents; D011524:Protoporphyrins; C028025:protoporphyrin IX