title: M phase-specific phosphorylation of histone H1.5 at threonine 10 by GSK-3.
abstract: H1 histones are progressively phosphorylated during the cell cycle. The number of phosphorylated sites is zero to three in late S phase and increases to five or six in late G2 phase and M phase. It is assumed that this phosphorylation modulates chromatin condensation and decondensation, but its specific role remains unclear. Recently, it was shown that the somatic H1 histone subtype H1.5 becomes pentaphosphorylated during mitosis, with phosphorylated threonine 10 being the last site to be phosphorylated. We have generated an antiserum specific for human H1.5 phosphorylated at threonine 10. Immunofluorescence labeling of HeLa cells with this antiserum revealed that the phosphorylation at this site appears in prometaphase and disappears in telophase, and that this hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin condensation is maximal. In search of the kinase responsible for the phosphorylation at this site, we found that threonine 10 of H1.5 can be phosphorylated by glycogen synthase kinase-3 in vitro, but not by cyclin-dependent kinase 1/cyclin B and cyclin-dependent kinase 5/p35, respectively. Furthermore, addition of specific glycogen synthase kinase-3 inhibitors led to a reduction in phosphorylation at this site both in vivo and in vitro.
authors.affiliation: Institute for Biochemistry and Molecular Cell Biology, Department of Molecular Biology, University of Göttingen, Germany. nhappel@gwdg.de
authors.forename: Nicole
authors.initials: N
authors.lastname: Happel
authors.forename: Stefan
authors.initials: S
authors.lastname: Stoldt
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schmidt
authors.forename: Detlef
authors.initials: D
authors.lastname: Doenecke
mesh_terms: D016203:CDC2 Protein Kinase / Q000378:metabolism; D002455:Cell Division; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D013912:Threonine / Q000378:metabolism*
chemical_list: D006657:Histones; D013912:Threonine; D051360:Cyclin-Dependent Kinase 5; D016203:CDC2 Protein Kinase; C495905:CDK5 protein, human; D038362:Glycogen Synthase Kinase 3