Presence of duplicate genes encoding a phylogenetically new subgroup of form I ribulose 1,5-bisphosphate carboxylase/oxygenase in Mycobacterium sp. strain JC1 DSM 3803.
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme of the Calvin reductive pentose phosphate cycle. Two sets of the structural genes for form I RubisCO were identified in Mycobacterium sp. strain JC1. The genes were clustered on the chromosome in the transcriptional order of cbbL-cbbS. Cloned cbbL-1 and cbbS-1 and cbbL-2 and cbbS-2 have open reading frames of 1431, 426, 1428, and 426 nucleotides, respectively. Primer extension analysis revealed that transcriptional start sites of cbbLS-1 and -2 genes were the nucleotides T and G located 99 and 41bp upstream of the cbbL start codons, respectively. CbbLS-1 and CbbLS-2 that were expressed in Escherichia coli exhibited RubisCO activity. A phylogeny of CbbL amino acid sequences revealed that the two enzymes in Mycobacterium sp. strain JC1 may form a new phylogenetic subgroup, type IE, in the 'red-like' group of the form I RubisCO family.
Park
Sae Woong
SW
Molecular Microbiology Laboratory, Department of Biology, Yonsei University, Seoul, Republic of Korea. swpark@yonsei.ac.kr
Hwang
Eun Ha
EH
Jang
Hak Sun
HS
Lee
Jae Ho
JH
Kang
Beom Sik
BS
Oh
Jeong Il
JI
Kim
Young Min
YM
D003001:Cloning, Molecular; D020131:Genes, Duplicate; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014158:Transcription, Genetic
D011994:Recombinant Proteins; D012273:Ribulose-Bisphosphate Carboxylase