title: Comparative proteomics of leaf, stem, and root tissues of synthetic Brassica napus.
abstract: Comparative proteomics was applied to three vegetative organs of Brassica napus, the leaf, stem, and root using 2-DE. Among the >1600 analyzed spots, 43% were found to be common to all three organs, suggesting the existence of a "basal" or ubiquitous proteome composed of housekeeping proteins. The green organs, leaf, and stem, were closely related (approximately 80% common spots) while the root displayed more organ-specific polypeptides (approximately 10%). Reference maps were established using MS, allowing the identification of 93, 385, and 266 proteins in leaf, stem, and root proteomes, respectively. Bioinformatic analyses were also performed; in silico functional categorization and cellular localization allow obtaining a precise picture of the cell molecular network within vegetative organs. These proteome maps can be explored using the PROTICdb software at the following address: http://bioinformatique.moulon.inra.fr/proticdb/web_view/.
authors.affiliation: Team Evolutionary Genetics: Adaptation and Redundancy, UMR 0320/UMR 8120 Génétique Végétale, INRA, Univ Paris-Sud, CNRS, AgroParisTech, Ferme du Moulon, Gif-sur-Yvette, France. albertin@moulon.inra.fr
authors.forename: Warren
authors.initials: W
authors.lastname: Albertin
authors.forename: Olivier
authors.initials: O
authors.lastname: Langella
authors.forename: Johann
authors.initials: J
authors.lastname: Joets
authors.forename: Luc
authors.initials: L
authors.lastname: Négroni
authors.forename: Michel
authors.initials: M
authors.lastname: Zivy
authors.forename: Catherine
authors.initials: C
authors.lastname: Damerval
authors.forename: Hervé
authors.initials: H
authors.lastname: Thiellement
mesh_terms: D029688:Brassica napus / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*