Identification of the immediate-early genes of white spot syndrome virus.
During viral infection, viral immediate-early (IE) genes encode regulatory proteins critical for the viral life cycle. Here we screened white spot syndrome virus (WSSV) IE genes with cycloheximide (CHX)-treated primary culture of crayfish hemocyte and a WSSV genome tiling microarray. Sixteen ORFs, including a known WSSV IE gene (ie1/wsv069), were identified and confirmed by RT-PCR and time course studies. The 16 identified IE proteins contain four proteins (wsv051, wsv069, wsv100, wsv079) with transcription activity, one (wsv083) with Ser/Thr kinase domain and one (wsv249) previously described to function as an ubiquitin E3 ligase. Furthermore, most of the identified WSSV IE genes cluster in a 14 kb genomic region (WSSV China isolate: 36,052 to 50,300 bp). This type of arrangement may facilitate the coordinate control and rapid expression of IE genes.
Li
Fang
F
Xiamen University, PR China.
Li
Mingyuan
M
Ke
Wei
W
Ji
Yongchang
Y
Bian
Xiaofang
X
Yan
Xiumin
X
D000818:Animals; D003400:Astacoidea / Q000821:virology*; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral; D023061:Gene Order; D017781:Genes, Immediate-Early / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D046848:White spot syndrome virus 1 / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
D017874:Immediate-Early Proteins