[Effect of glycyrrhetinic acid on invasive capacity of leukemic cells and activity of gelatinase].
OBJECTIVE
To study the effect of glycyrrhetinic acid (GA) on the invasive capacity of leukemic cells and the activity of intracellular gelatinase.


METHODS
The effect of GA, in different concentrations, on the proliferation of cultured K562 and HL-60 leukemic cells in vitro was determined by MTT assay; that on cell invasive capacity was tested by Transwell cubicle matrigel invasion assay; and that on the activity of gelatinase in cells was detected by gelatin zymography.


RESULTS
GA showed significant inhibitory effect on the proliferation of K562 and HL-60 leukemic cells; it inhibited the invasive capacity of cells in concentration-dependent manner; and significantly down-regulated the activity of gelatinase A and B in cells.


CONCLUSIONS
GA can inhibit invasive capacity of K562 and HL-60 leukemia cells by way of suppressing the activity of gelatinase A and B. This study provides an experimental evidence for preventing extra-medullary infiltration of leukemic cells.
Zhang
Jin-Ling
JL
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan.
Liu
Xin-Yue
XY
Zou
Ping
P
D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D018093:Gelatinases / Q000378:metabolism*; D006034:Glycyrrhetinic Acid / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology* / Q000503:physiopathology; D009361:Neoplasm Invasiveness
D018093:Gelatinases; D006034:Glycyrrhetinic Acid