Development of an acrylate monolith in a cyclo-olefin copolymer microfluidic device for chip electrochromatography separation.
An acrylate monolith has been synthesized into a cyclic olefin copolymer microdevice for reversed-phase electrochromatography purposes. Microchannels, designed by hot embossing, were filled up with an acrylate monolith to serve as a hydrophobic stationary phase. A lauryl acrylate monolith was formulated to suit the hydrophobic material, by implementing 100% organic porogenic solvent. This new composition was tested in capillary prior to its transfer into the microfluidic device. Surface functionalization of the cyclic olefin copolymer surface was applied using UV-grafting technique to improve the covalent attachment of this monolith to the plastic walls of the microfluidic chip. The on-chip performances of this monolith were evaluated in detail for the reversed-phase electrochromatographic separation of polycyclic aromatic hydrocarbons, with plate heights reaching down to 10 microm when working at optimal velocity.
Faure
Karine
K
Laboratoire des Sciences Analytiques, Université de Lyon, Villeurbanne, France. karine.faure@univ-lyon1.fr
Albert
Monique
M
Dugas
Vincent
V
Crétier
Gérard
G
Ferrigno
Rosaria
R
Morin
Pierre
P
Rocca
Jean-Louis
JL
D000179:Acrylates / Q000737:chemistry*; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods*; D003516:Cycloparaffins / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results
D000179:Acrylates; D003516:Cycloparaffins; D011084:Polycyclic Aromatic Hydrocarbons; D011108:Polymers; C036658:acrylic acid