title: [Levels of PCDD/Fs, PCBs and PBDEs compounds in human placenta tissue].
abstract: OBJECTIVE
  To establish the methods of Polychlorinated Dibenzo-p-Dioxins and Dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) compounds determination by isotope dilution HRGC/HRMS simultaneously in human placenta tissue from mothers, and assess the human exposure risk to dioxins and PBDEs in study.
  
  
  METHODS
  Concentrations of 17 PCDD/Fs and 12 dioxin-like PCBs as well as 7 PBDEs were measured in human placenta tissue samples by isotope dilution HRGC/HRMS. SigmaTEQ (PCDD + PCDFs + PCBs) concentration using WHO-TEF factor and PBDEs concentration was calculated respectively. Risk assessment of mother exposure to dioxins and PBDEs was evaluated.
  
  
  RESULTS
  Median of SigmaTEQ (PCDD + PCDFs + PCBs) concentration for six samples was 18.15 WHO-TEQ pg/g lipid, ranging from 5.14 - 67.01 WHO-TEQ pg/g lipid. Although the median of SigmaTEQ (PCDD + PCDFs + PCBs) was lower than that of human blood of EU and Japan, and close to that of Korea and Taiwan non-exposure as reported in the literatures, the highest SigmaTEQ (PCDD + PCDFs + PCBs) concentration of placenta sample exceeded the value of high dioxins exposure area subjects in Taiwan. The dominant contributor congener for WHO-TEQ were 2, 3, 4, 7, 8-PeCDF, 1, 2, 3, 7, 8-PeCDD, PCB126, totally accounted for 65 percent of SigmaWHO-TEQ. Median and average of PBDE concentration for six samples were 2.73 ng/g lipid and 7.17 ng/g lipid, respectively, ranging from 0.95 - 25.99 ng/g lipid. BDE47 was the dominant contributor congener for the total concentration, accounted for 35 percent.
  
  
  CONCLUSIONS
  The methods of PCDD/Fs, PCBs and PBDEs compounds determined by isotope dilution HRGC/HRMS simultaneously in human placenta tissue from mothers were established successfully, and the human exposure risk to PCDD/Fs, PCBs and PBDEs should be surveyed for the donor with the highest SigmaTEQ (PCDD + PCDFs + PCBs) and PBDEs concentration of placenta sample in the future.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Xiao-kang
authors.initials: XK
authors.lastname: Sun
authors.forename: You-sheng
authors.initials: YS
authors.lastname: Jiang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Li-bin
authors.initials: LB
authors.lastname: Wang
authors.forename: Zhao-yi
authors.initials: ZY
authors.lastname: Ye
authors.forename: Dao-kui
authors.initials: DK
authors.lastname: Fang
authors.forename: Guo-bin
authors.initials: GB
authors.lastname: Wang
mesh_terms: D001572:Benzofurans / Q000032:analysis; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000032:analysis*; D006801:Humans; D018811:Maternal Exposure; D010920:Placenta / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D011247:Pregnancy
chemical_list: D001572:Benzofurans; D055768:Halogenated Diphenyl Ethers; D000072317:Polychlorinated Dibenzodioxins; C023614:dibenzofuran; D011078:Polychlorinated Biphenyls