Selective recognition of unnatural imidazopyridopyrimidine:naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment.
In this work, we investigated how thermally stable ImO(N):NaN(O) and ImN(O):NaO(N) pairs are recognized by the Klenow fragment (KF). As a result, these complementary base pairs, especially the ImN(O):NaO(N) pair, were recognized selectively due to the four hydrogen bonds between the nucleobases and the shape complementarity of the Im:Na pair similar to the purine:pyrimidine base pair.
Minakawa
Noriaki
N
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Ogata
Shintaro
S
Takahashi
Mayumi
M
Matsuda
Akira
A
D020029:Base Pairing; D004256:DNA Polymerase I / Q000737:chemistry*; D006860:Hydrogen Bonding; D007093:Imidazoles / Q000737:chemistry; D007700:Kinetics; D009287:Naphthyridines / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*
D007093:Imidazoles; D009287:Naphthyridines; D009711:Nucleotides; D011743:Pyrimidines; D004256:DNA Polymerase I