Identification of marbling-related candidate genes in M. longissimus dorsi of high- and low marbled Hanwoo (Korean Native Cattle) steers.
This study was conducted to identify marbling-related candidate genes in M. longissimus dorsi of high- and low-marbled Hanwoo. The longissimus dorsi muscles were selected for gene expression from eight Hanwoo steer carcasses based on crude fat content. In the analysis of variance, gene expression of five candidate genes, FABP4, SCD, PPARgamma, Titin and Nebulin was determined to be significantly different between high- and low-marbled Hanwoo steers (P < 0.0001). The Pik-4 and CaMK II genes were also shown to have a significant effect on crude fat content (P < 0.01). In the analysis of the differential expression between high- and low marbled groups, FABP4 gene expression was approximately 2 times higher in the high marbled group relative to the low marbled group. However, the PPARgamma and SCD gene were highly expressed in the low marbled group. In addition, Titin and Nebulin were highly expressed in the low marbled group when placed under relatively high shear force. Finally, the Pik-4 and CaM K II gene also displayed a high expression pattern in the low marbled group.
Lee
Seung-Hwan
SH
National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.
Cho
Yong-Min
YM
Lee
Sang-Hong
SH
Kim
Bum-Soo
BS
Kim
Nam-Kuk
NK
Choy
Yeon-Ho
YH
Kim
Kyoung-Hoon
KH
Yoon
Duhak
D
Im
Seok-Ki
SK
Oh
Sung-Jong
SJ
Park
Eung-Woo
EW
D000273:Adipose Tissue / Q000033:anatomy & histology*; D000367:Age Factors; D000818:Animals; D050218:Body Fat Distribution; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics; D002417:Cattle / Q000033:anatomy & histology* / Q000235:genetics*; D064211:Connectin; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D007723:Korea; D008297:Male; D008460:Meat; D015778:Minor Histocompatibility Antigens; D009124:Muscle Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000033:anatomy & histology*; D047495:PPAR gamma / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013230:Stearoyl-CoA Desaturase / Q000235:genetics
D064211:Connectin; D050556:Fatty Acid-Binding Proteins; D015778:Minor Histocompatibility Antigens; D009124:Muscle Proteins; D047495:PPAR gamma; C035620:nebulin; D013230:Stearoyl-CoA Desaturase; D011494:Protein Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); C402182:phosphatidylinositol phosphate 4-kinase; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2