Chitosan microparticles loaded with mite group 2 allergen Der f 2 alleviate asthma in mice.
BACKGROUND
In recent years, nanoparticle materials have found wide applications in drug transport and release systems. Chitosan is a good carrier for proteins, peptides, and nucleic acids because of its favorable release properties and ability to increase membrane permeability.


OBJECTIVE
To investigate the effect of chitosan microparticles loaded with the major epitope peptide of mite group 2 allergen Der f 2 from Dermatophagoides farinae (Der f 2-47-67) in alleviating asthma in mice.


METHODS
Derf 2-47-67 was entrapped in chitosan to obtain Derf 2-47-67-loaded chitosan microparticles, which were injected intraperitoneally into BALB/c mice prior to an intranasal challenge with a Der f extract allergen. Airway hyperreactivity was measured via whole-body plethysmography, and bronchoalveolar lavage (BAL) fluid was collected to calculate total cell and eosinophil counts. Changes in lung histology were assessed after hematoxylin-eosin staining, and serum levels of Der f-specific immunoglobulin (Ig) G2a and IgE were determined by enzyme-linked immunoabsorbent assay.


RESULTS
Mice immunized with Der f 2-47-67-loaded chitosan microparticles displayed decreased airway hyperreactivity, reduced numbers of eosinophils in BAL fluid, alleviated lung inflammation and mucus production, a reduced serum level of Der f-specific IgE and an increased serum level of Der f-specific IgG2a.


CONCLUSIONS
Our data showed that Der f 2-47-67-loaded chitosan microparticles inhibited airway allergic inflammation.
Li
J
J
Allergy and Immunology Institute, Shenzhen University School of Medicine, Shenzhen University, Shenzhen, China.
Liu
Z
Z
Wu
Y
Y
Wu
H
H
Ran
P
P
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000008:administration & dosage* / Q000276:immunology; D060829:Arthropod Proteins; D001249:Asthma / Q000628:therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D048271:Chitosan; D016503:Drug Delivery Systems; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C
D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451160:Dermatophagoides farinae antigen f 2; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D048271:Chitosan