title: Term labor is associated with a core inflammatory response in human fetal membranes, myometrium, and cervix.
abstract: OBJECTIVE
  Identify overlap of the transcriptome of myometrium and cervix in association with human labor.
  
  
  METHODS
  The transcriptional profile of myometrial and cervical biopsies obtained from women in spontaneous labor at term (n = 9) and not in labor (n = 9) were characterized by Affymetrix v2 U133 plus 2 arrays. Common canonical pathways and functional groups were identified by Ingenuity Pathway Analysis.
  
  
  RESULTS
  One hundred ten genes (false discovery rate < 1%) were commonly up-regulated by myometrium and cervix in association with labor and 29 genes (false discovery rate < 1%) down-regulated. Fold change in expression of up-regulated genes was strongly correlated; myometrium vs cervix (r = 0.51; P < .001), with no relationship in down-regulated genes (r = 0.26; P = .16). Canonical pathway analysis established up-regulation of inflammatory pathway signaling, with greatest increases in cellular movement and immune response gene ontology groups.
  
  
  CONCLUSIONS
  Gestational tissues exhibit a core inflammatory response in association with human parturition, with pathways regulating cellular trafficking dominating.
authors.affiliation: Division of Reproductive and Maternal Medicine, Faculty of Medicine, University of Glasgow, Glasgow, Scotland.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Bollapragada
authors.forename: Shrikant
authors.initials: S
authors.lastname: Bollopragada
authors.forename: Refaat
authors.initials: R
authors.lastname: Youssef
authors.forename: Fiona
authors.initials: F
authors.lastname: Jordan
authors.forename: Ian
authors.initials: I
authors.lastname: Greer
authors.forename: Jane
authors.initials: J
authors.lastname: Norman
authors.forename: Scott
authors.initials: S
authors.lastname: Nelson
mesh_terms: D000328:Adult; D001706:Biopsy; D002584:Cervix Uteri / Q000502:physiology*; D005321:Extraembryonic Membranes / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007743:Labor, Obstetric / Q000502:physiology*; D009215:Myometrium / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D055815:Young Adult
chemical_list: D012313:RNA