title: Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion.
abstract: OBJECTIVE
  Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesized that within this time, inflammatory cytokines might accumulate.
  
  
  METHODS
  Serial plasma samples were collected 4, 10 and 20 h after donation and cytokine concentrations were measured.
  
  
  RESULTS
  Interleukin-8 increased > 20-fold and soluble CD40 ligand > sixfold during the observation time, less pronounced changes for several other mediators were also observed.
  
  
  CONCLUSIONS
  Leucocyte depletion within 10 h of blood donation will reduce the concentrations of pyrogenic mediators.
authors.affiliation: German Red Cross Blood Centre and Institute for Transfusion Medicine and Immunohaematology of the Johann-Wolfgang-Goethe University, Frankfurt, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Chudziak
authors.forename: W
authors.initials: W
authors.lastname: Sireis
authors.forename: H-U
authors.initials: HU
authors.lastname: Pfeiffer
authors.forename: R
authors.initials: R
authors.lastname: Henschler
authors.forename: E
authors.initials: E
authors.lastname: Seifried
authors.forename: H
authors.initials: H
authors.lastname: Bönig
mesh_terms: D001782:Blood Donors; D001793:Blood Preservation; D005334:Fever / Q000209:etiology; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D047589:Leukocyte Reduction Procedures; D012995:Solubility; D013997:Time Factors; D065227:Transfusion Reaction
chemical_list: D018836:Inflammation Mediators