[An enzyme electrode biosensor for inosine determination].
An enzyme electrode biosensor was used for the amperometric determination of inosine in its tablets by co-immobilizing nucleoside phosporylase and xanthine oxidase on a hydrogen peroxide electrode. As a fundamental electrode the hydrogen peroxide electrode has an advantage of stability in analysis compared with the 02 electrode. The enzyme electrode showed a linear response to inosine in the range of 1-268 mg/L with a response of 60 seconds under a sample injection volume of 25 microL. Based on the enzyme electrode, inosine solutions were determined with an average recover rate of 100.8% and a relative standard deviation (RSD) of les than 0.14% in 20 assays. The lifetime of the enzyme electrode was relative long and could be used continuously at 25 degrees C for 25 days. These results demonstrated that the enzyme electrode biosensor could be used to determine inosine and its derivatives specifically, rapidly, conveniently and economically.
Sun
Shiqing
S
Adrrssed Institute of Biology, Shandong Academy of Sciences, Shandong Key Laboratory for Biosensors, Ji'nan 250014, China sundaiman77@yahoo.com.cn
Shi
Jianguo
J
Li
Xuemei
X
Yang
Junhui
J
Ma
Yaohong
Y
Meng
Qingjun
Q
Sun
Dai
D
D015374:Biosensing Techniques / Q000379:methods*; D007288:Inosine / Q000032:analysis*; D012680:Sensitivity and Specificity
D007288:Inosine