Multiplex integrating waveguide sensor: signalyte-II.
A platform to detect multiplex fluorescent labels was developed based on liquid phase implementation of the Integrating Waveguide Sensor detection principles. The liquid sample is held in a capillary cuvette with a lens at one end. The excitation light incident on the cuvette at 90 degrees angle. The emitted fluorescence is efficiently gathered and propagated to the end of the waveguide cuvette, exiting via the lens to the detector. The capillary cuvette acts as a waveguide to efficiently gather the emission signal, providing high detection sensitivity for small sample sizes. Excitation sources ranging from 470 to 635 nm are four high-powered LEDs, allowing for multiplex fluorescence assays and a spectrometer is used to collect the signal from 390 to 790 nm. The cuvette can hold 1-35 microL samples. This technology can be used for a wide variety of assays and detection needs, such as FRET, end point PCR reading, immunoassays, chemiluminescence detection, multiplex quantum dots assays, polarization assays, etc.
Li
Shuhong
S
Creatv MicroTech Inc, Potomac, MD, USA.
Zhang
Yunqi
Y
Amstutz
Platte
P
Tang
Cha-Mei
CM
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D018511:Systems Integration