title: Influence of microleakage, surface roughness and biofilm control on secondary caries formation around composite resin restorations: an in situ evaluation.
abstract: This study was carried out to evaluate in situ the influence of microleakage, surface roughness and biofilm control on caries formation around composite resin restorations. During 28 days, 12 volunteers wore palatal devices containing bovine enamel slabs restored with composite resin. Restorations were made without leakage, when the adhesive system was applied, or with leakage, when adhesive system was omitted. Half of the restorations in each group were finished and the remaining were finished and polished. In one side of the palatal device, biofilm was left to accumulate over the restored slabs, and in the other side dental slabs were brushed, to allow biofilm removal. There was an extraoral application of 20% sucrose solution (8x/day) over the enamel slabs. The formation of caries lesions (white spots) was evaluated by visual inspection under stereomicroscopy. Additionally, the dental slabs were sectioned and observed under polarized light microscopy. Data were submitted to Kruskal-Wallis test and Spearman's correlation test at 5% significance level. Polishing and bonding were not significant factors regarding white spot formation (p>0.05). Biofilm control (brushing) was associated with reduction of caries formation close to the restorations (p<0.01). Polarized light microscopy confirmed the visual inspection findings. These results suggest that while microleakage and surface roughness did not influence caries lesion formation, biofilm control may prevent the enamel demineralization.
authors.affiliation: Department of Operative Dentistry, University of Western Santa Catarina, Joaçaba, SC, Brazil.
authors.forename: Fábio Garcia
authors.initials: FG
authors.lastname: Lima
authors.forename: Ana Regina
authors.initials: AR
authors.lastname: Romano
authors.forename: Marcos Britto
authors.initials: MB
authors.lastname: Correa
authors.forename: Flávio Fernando
authors.initials: FF
authors.lastname: Demarco
mesh_terms: D000328:Adult; D000818:Animals; D018441:Biofilms; D017438:Bisphenol A-Glycidyl Methacrylate; D002417:Cattle; D003188:Composite Resins; D003731:Dental Caries / Q000209:etiology* / Q000517:prevention & control*; D003763:Dental Leakage / Q000150:complications; D003773:Dental Plaque / Q000628:therapy; D003775:Dental Polishing; D003793:Dental Restoration, Permanent / Q000009:adverse effects* / Q000379:methods; D006801:Humans; D008297:Male; D012008:Recurrence; D019279:Resin Cements; D013499:Surface Properties; D014099:Toothbrushing; D055815:Young Adult
chemical_list: D003188:Composite Resins; C444701:Filtek Z250; D019279:Resin Cements; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate