title: In vitro effects of Cucurbita moschata seed extracts on Haemonchus contortus.
abstract: This study was carried out to evaluate the in vitro effect of Cucurbita moschata seed against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and dichloromethane) of C. moschata seed were tested in vitro on four developmental stages of H. contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition (LMI) assay and adult worm motility (AWM) assay. The highly significant (P<0.001) ability to stop larval development (inhibition>90% for each extract) and the negative effect of the dichloromethane and methanolic extracts on adult worm motility (inhibition of motility >59.2% after 24h of incubation) compared to the negative controls, suggest anthelmintic properties of C. moschata seed against H. contortus. The active principles responsible for the activity could be secondary metabolites such as amino acid compounds or terpenoid compounds present in the extracts.
authors.affiliation: Institut National de la Recherche Agronomique, Unité de Recherches Zootechniques, Domaine de Duclos, 97170 Prise d'eau PETIT-BOURG, Guadeloupe (FWI), Guadeloupe. Carine.Marie-Magdeleine@antilles.inra.fr
authors.forename: C
authors.initials: C
authors.lastname: Marie-Magdeleine
authors.forename: H
authors.initials: H
authors.lastname: Hoste
authors.forename: M
authors.initials: M
authors.lastname: Mahieu
authors.forename: H
authors.initials: H
authors.lastname: Varo
authors.forename: H
authors.initials: H
authors.lastname: Archimede
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000737:chemistry* / Q000494:pharmacology*; D028464:Cucurbita / Q000737:chemistry*; D006189:Haemonchus / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D010063:Ovum / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts