title: Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora.
abstract: Continuous hydrogen production from glucose at 75 degrees C was examined at HRTs of 0.67, 1, 2, and 3 d by using extreme thermophilic microflora, which consisted of several subspecies of Caldanaerobacter subterraneus. The maximum hydrogen yield of 3.32 mol-H2/mol-glucose degraded was obtained at the highest HRT, 3 d. Both the maximum volumetric and specific rates of hydrogen production (1.22 L-H2/L/d and 4.15 L-H2/g-VSS/d, respectively) were obtained at the lowest HRT, 0.67 d. A 16S rDNA analysis showed that the community structure of the microflora did not change at any of the HRTs.
authors.affiliation: Waste Recycling Research Team, National Institute of Livestock and Grassland Science (NILGS), 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan. hiroshiy@affrc.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ohmori
authors.forename: Miyoko
authors.initials: M
authors.lastname: Waki
authors.forename: Akifumi
authors.initials: A
authors.lastname: Ogino
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D006859:Hydrogen / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D013696:Temperature; D014865:Waste Disposal, Fluid
chemical_list: D012336:RNA, Ribosomal, 16S; D006859:Hydrogen; D005947:Glucose