Production of apoptosis-inducing substances from soybean protein by Clostridium butyricum: characterization of their toxic effects on human colon carcinoma cells.
Microbial metabolism of soybean constituents is known to produce novel active substances as a chemopreventive agent during the fermentation, and enterobacteria are expected to produce chemopreventive agents as a consequence of metabolizing soybean constituents in the intestinal tract. Then, the conditioned medium was prepared by culturing an enterobacterium Clostridium butyricum (C. butyricum) with soybean protein, and its direct effect on human colon carcinoma HCT116 cells was examined. The conditioned medium was shown to induce the cell death, and suggested to contain novel apoptosis-inducing substances. Thus, enterobacteria are predicted to produce anti-tumor substances from food-derived proteins within the intestinal tract.
Arimochi
Hideki
H
Department of Molecular Bacteriology, Tokushima University School of Medicine, Kuramoto, Tokushima, Japan. arimochi@basic.med.tokushima-u.ac.jp
Morita
Kyoji
K
Nakanishi
Shusuke
S
Kataoka
Keiko
K
Kuwahara
Tomomi
T
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis / Q000494:pharmacology*; D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D046972:Clostridium butyricum / Q000378:metabolism*; D003110:Colonic Neoplasms; D017077:Culture Media, Conditioned; D045325:HCT116 Cells; D006801:Humans; D030262:Soybean Proteins / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D017077:Culture Media, Conditioned; D030262:Soybean Proteins