Carbohydrate binding specificity of recombinant human macrophage beta-glucan receptor dectin-1.
Human macrophage dectin-1, a type II transmembrane beta-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and assayed for binding specificity. Recombinant dectin-1 specifically bound to some beta-glucans, but not to other carbohydrates. The beta-glucan binding of recombinant dectin-1 was inhibited by laminarin, a soluble beta-glucan, and by laminarioligosaccharides, but not by other carbohydrates. These results suggest that recombinant human dectin-1 can be used as a useful probe in identifying ligands in humans and tonic foods due to its strict binding specificity.
Ujita
Minoru
M
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University. ujita@ccmfs.meijo-u.ac.jp
Nagayama
Hiroko
H
Kanie
Satoko
S
Koike
Shota
S
Ikeyama
Yoshiko
Y
Ozaki
Takahiro
T
Okumura
Hiroki
H
D002241:Carbohydrates; D005982:Glutathione Transferase; D006801:Humans; D037181:Lectins, C-Type; D008264:Macrophages / Q000737:chemistry*; D008565:Membrane Proteins / Q000378:metabolism*; D015336:Molecular Probe Techniques; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D047071:beta-Glucans / Q000378:metabolism; D000099181:Dectin-1
D002241:Carbohydrates; D005982:Glutathione Transferase; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D047071:beta-Glucans; D000099181:Dectin-1; C045268:beta-glucan receptor