title: Tissue-engineered skin containing mesenchymal stem cells improves burn wounds.
abstract: There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue-engineered (TE) skin combined with bone MSCs, we established a burn wound model in the porcine skin. With a controlling temperature and time of the burning device to obtain different degrees of burn wounds, a deep dermal partial thickness burn was introduced to the porcine skin using a heated-brass contact injury at 100 degrees C for 20 s. Collagen-GAG scaffolds were utilized as the matrix; MSCs separated from pigs were seeded on them to form the skin equivalent. When grafted to the burn wounds, the TE skin containing MSCs showed better healing and keratinization, less wound contraction, and more vascularization. Grafts proliferated well and contributed to the neo-tissues. These data suggest that TE skin containing MSCs in a burn defect can accelerate wound healing and receive satisfactory effects.
authors.affiliation: Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Deng
authors.forename: Shufang
authors.initials: S
authors.lastname: Han
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Wen
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Xianhui
authors.initials: X
authors.lastname: Geng
authors.forename: Sha
authors.initials: S
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002056:Burns / Q000601:surgery*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D023421:Models, Animal; D012867:Skin / Q000473:pathology / Q000648:ultrastructure; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
chemical_list: D003094:Collagen