Engineered Saccharomyces cerevisiae that produces 1,3-propanediol from D-glucose.
OBJECTIVE
Saccharomyces cerevisiae is a safe micro-organism used in fermentation industry. 1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3-propanediol at low cost.


RESULTS
By using D-glucose as a feedstock, S. cerevisiae could produce glycerol, but not 1,3-propanediol. In this study, we have cloned two genes yqhD and dhaB required for the production of 1,3-propanediol from glycerol, and integrated them into the chromosome of S. cerevisiae W303-1A by Agrobacterium tumefaciens-mediated transformation. Both genes yqhD and dhaB functioned in the engineered S. cerevisiae and led to the production of 1,3-propanediol from D-glucose.


CONCLUSIONS
Saccharomyces cerevisiae can be engineered to produce 1,3-propanediol from low-cost feedstock D-glucose.


CONCLUSIONS
To our knowledge, this is the first report on developing S. cerevisiae to produce 1,3-propanediol by using A. tumefaciens-mediated transformation. This study might lead to a safe and cost-efficient method for industrial production of 1,3-propanediol.
Rao
Z
Z
The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, PR China. raozm@yahoo.com.cn
Ma
Z
Z
Shen
W
W
Fang
H
H
Zhuge
J
J
Wang
X
X
D000818:Animals; D005285:Fermentation / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D011409:Propylene Glycols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014169:Transformation, Bacterial
D011409:Propylene Glycols; D029701:Saccharomyces cerevisiae Proteins; C041787:1,3-propanediol; D005947:Glucose