title: Influence of glutaraldehyde priming on bond strength of an experimental adhesive system applied to wet and dry dentine.
abstract: OBJECTIVE
  This study tested the following null hypotheses: (1) there is no difference in resin-dentine bond strength when an experimental glutaraldehyde primer solution is added prior to bonding procedures and (2) there is no difference in resin-dentine bond strength when experimental glutaraldehyde/adhesive system is applied under dry or wet demineralized dentine conditions.
  
  
  METHODS
  Extracted human maxillary third molars were selected. Flat, mid-coronal dentine was exposed for bonding and four groups were formed. Two groups were designated for the dry and two for the wet dentine technique: DRY: (1) Group GD: acid etching+glutaraldehyde primer (primer A)+HEMA/ethanol primer (primer B)-under dried dentine+unfilled resin; (2) Group D: the same as GD, except for primer A application; WET: (3) Group GW: the same as GD, but primer B was applied under wet dentine condition; (4) Group W: the same as GW, except for primer A application. The bonding resin was light-cured and a resin core was built up on the adhesive layer. Teeth were then prepared for microtensile bond testing to evaluate bond strength. The data obtained were submitted to ANOVA and Tukey's test (alpha=0.05).
  
  
  RESULTS
  Glutaraldehyde primer application significantly improved resin-dentine bond strength. No significant difference was observed when the same experimental adhesive system was applied under either dry or wet dentine conditions. These results allow the first null hypothesis to be rejected and the second to be accepted.
  
  
  CONCLUSIONS
  Glutaraldehyde may affect demineralized dentine properties leading to improved resin bonding to wet and dry substrates.
authors.affiliation: Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru Dental School, University of São Paulo, Av Octavio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil. r.cilli@yahoo.com
authors.forename: Renato
authors.initials: R
authors.lastname: Cilli
authors.forename: Anuradha
authors.initials: A
authors.lastname: Prakki
authors.forename: Paulo Amarante
authors.initials: PA
authors.lastname: de Araújo
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Pereira
mesh_terms: D000134:Acid Etching, Dental; D003188:Composite Resins; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D001840:Dental Bonding / Q000379:methods*; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D003890:Desiccation; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000494:pharmacology; D019279:Resin Cements / Q000737:chemistry*; D013718:Tensile Strength; D014867:Water
chemical_list: D003188:Composite Resins; D003432:Cross-Linking Reagents; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D019279:Resin Cements; D014867:Water; C005044:hydroxyethyl methacrylate; D005976:Glutaral