Combinatorial evaluation of cations, pH-sensitive and hydrophobic moieties for polymeric vector design.
Three combinatorial libraries of polymeric vectors were evaluated to investigate the functional roles of molecular weight (MW), cations, pH-sensitive moieties, and hydrophobic derivitization in polymer-mediated gene delivery. Four cationic and pH-sensitive moieties (imidazole, primary, secondary, and tertiary amino) and three hydrophobic residues (C4 butyl, C6 hexyl, and C8 octyl) were assessed in single and serially incremented, binary combinations. Three MWs were evaluated-10, 30, and 50 kDa. The highest levels of transfection, comparable to branched PEI (25 kDa), were achieved by 30 kDa and 50 kDa formulations containing primary amino and imidazole groups. Primary amino groups offered superior charge-neutralizing and size-condensing capacity, while imidazole groups appeared to bind with DNA via nonelectrostatically mediated interactions to produce stable polyplexes that were resistant to premature dissociation. Eight of the 10 highest-transfecting polymers possessed IC(50) values greater than the maximum concentration of free polymers exposed to cells (200 microg/ml). The results herein have identified highly efficient polymeric formulations with superb toxicity profiles and have revealed the functional roles that the investigated pendant groups play in the transfection process. The reported polymeric system offers a versatile and robust platform upon which future structure-function studies may be based to create safer and more efficient polymeric vectors.
Wong
Sharon Y
SY
1Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, USA.
Sood
Nimil
N
Putnam
David
D
D000818:Animals; D055592:Biophysical Phenomena; D002412:Cations / Q000737:chemistry; D005822:Genetic Vectors / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D019076:Transgenes / Q000235:genetics
D002412:Cations; D011108:Polymers