Simultaneous detection of Haemophilus influenzae type b polysaccharide-specific antibodies and Neisseria meningitidis serogroup A, C, Y, and W-135 polysaccharide-specific antibodies in a fluorescent-bead-based multiplex immunoassay.
We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R=0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.
de Voer
Richarda M
RM
Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Antonie van Leeuwenhoeklaan 1, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands. Richarda.de.Voer@rivm.nl
Schepp
Rutger M
RM
Versteegh
Florens G A
FG
van der Klis
Fiona R M
FR
Berbers
Guy A M
GA
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D003429:Cross Reactions; D005453:Fluorescence; D019741:Haemophilus influenzae type b / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D008863:Microspheres; D038381:Neisseria meningitidis, Serogroup A / Q000276:immunology*; D038542:Neisseria meningitidis, Serogroup C / Q000276:immunology*; D038582:Neisseria meningitidis, Serogroup W-135 / Q000276:immunology*; D038581:Neisseria meningitidis, Serogroup Y / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial