title: Urinary prostasin: a possible biomarker for renal pressure natriuresis in black adolescents.
abstract: Prostasin is a membrane-bound/secretive serine protease interacting with aldosterone and the epithelial sodium channel in the kidney. We and others have previously proposed the concept of stress-induced pressure natriuresis (SIPN) where increased urinary sodium excretion (UNaV) is coupled with elevated blood pressure (BP) in response to behavioral stress in normotensive adolescents. This study thus aimed to test the relationship between prostasin and pressure natriuresis using the SIPN model. A cohort of 102 normotensive black adolescents (mean age: 17.0 +/- 1.2 y; 56% females) were placed on a controlled sodium (4000 +/- 200 mg/d) and potassium (2600 +/- 200 mg/d) diet for three days before testing. The SIPN protocol consisted of a 1-h baseline period, a 1-h stress period (competitive video game), and a 1-h recovery period. During the stress period, BP elevation was coupled with an increase in UNaV. Urinary prostasin concentration had more than a 2-fold reduction from baseline (38.4 +/- 32.7 ng/mL) to stress (17.2 +/- 16.0 ng/mL), and further declined during recovery (12.1 +/- 16.2 ng/mL) (p < 0.001). Urinary prostasin was inversely correlated with UNaV during stress (r = -0.43, p = 0.0001), even after being normalized by urinary creatinine. Our data suggest that urinary prostasin could be a novel biomarker and/or mechanism for renal pressure natriuresis in normotensive black adolescents.
authors.affiliation: Department of Pediatrics, Medical College of Georgia, Augusta, Georgia 30907, USA.
authors.forename: Haidong
authors.initials: H
authors.lastname: Zhu
authors.forename: Julie
authors.initials: J
authors.lastname: Chao
authors.forename: Dehuang
authors.initials: D
authors.lastname: Guo
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Kimberly
authors.initials: K
authors.lastname: Hawkins
authors.forename: Nikki
authors.initials: N
authors.lastname: Wright
authors.forename: Inger
authors.initials: I
authors.lastname: Stallmann-Jorgensen
authors.forename: Weili
authors.initials: W
authors.lastname: Yan
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Harshfield
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000293:Adolescent; D001741:Black or African American; D000450:Aldosterone / Q000097:blood; D015415:Biomarkers / Q000652:urine; D001794:Blood Pressure; D003404:Creatinine / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D009318:Natriuresis; D011188:Potassium / Q000097:blood; D012697:Serine Endopeptidases / Q000652:urine*; D012964:Sodium / Q000097:blood; D013315:Stress, Psychological / Q000503:physiopathology / Q000652:urine*; D013997:Time Factors; D000099271:Prostasin
chemical_list: D000450:Aldosterone; D015415:Biomarkers; D003404:Creatinine; D011188:Potassium; D012697:Serine Endopeptidases; D012964:Sodium; D000099271:Prostasin