[Somatic hybrids among transgenic Solanum tuberosum and transplastomic Solanum rickii].
The hybrid plants with transformed plastids were regenerated after fusion of mesophyll protoplasts of Solanum rickii with aadA chloroplast selective marker gene and Solanum tuberosum with nptII nuclear selective marker gene. The hybrid callus clones were selected on the medium containing streptomycine, spectinomycine and kanamycine. The hybrids were identified on the base of PCR analysis of nuclear and plastid DNAs. The analysis have shown that regenerated plants were somatic hybrids containing aadA and nptII genes, Solanum rickii and Solanum tuberosum nuclear and chloroplast DNA.
Marveeva
N A
NA
Shakhovskiĭ
A M
AM
Kuchuk
N V
NV
D002467:Cell Nucleus / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics; D046508:Culture Techniques; D018742:DNA, Chloroplast / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D017343:Genes, Plant; D006824:Hybridization, Genetic; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D011523:Protoplasts; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development
D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial