[Changes of nuclear matrix proteins during apoptosis of human osteosarcoma MG-63 cells induced by curcumin].
Human osteosarcoma MG-63 cells were induced into apoptosis by curcumin (Cur). Its nuclear matrix proteins were selectively extracted, and subjected to two dimensional gel electrophoresis analysis. The resulted protein patterns were analyzed by Melanie software. There were 27 spots changed remarkably during the apoptosis induced by curcumin, 21 of which were identified. There were seven up-regulated proteins including DNA polymerase zeta and forteen down-regulated proteins including Prohibitin. This study suggests that the induced apoptosis of carcinoma cells is accompanied with changes of nuclear matrix proteins, and confirms the presence of some specific nuclear matrix proteins associated with carcinoma cell growth, apoptosis and the associated signal transduction pathways in induced apoptosis of carcinoma cells. It provides proofs and a new way to study the mechanisms of gene expression carcinogenesis which reveal the relationship between configuration and composition of nuclear matrix and cell apoptosis. Besides, this study provides several potential target proteins for cancer diagnosis and cancer therapy.
Li
Qi Fu
QF
Laboratory of Cell Biology, Key Laboratory of Ministry of Education for Cell Biology & Tumor cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China. chifulee@xmu.edu.cn
Zheng
Yan Bin
YB
Yang
Hai Bo
HB
Shi
Song Lin
SL
Zhao
Zhen Li
ZL
Chen
Jin An
JA
D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D046529:Microscopy, Electron, Transmission; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure
D034521:Nuclear Matrix-Associated Proteins; D003474:Curcumin