Freeze-drying of proteins in glass solids formed by basic amino acids and dicarboxylic acids.
The purpose of this study was to produce and characterize glass-state amorphous solids containing amino acids and organic acids that protect co-lyophilized proteins. Thermal analysis of frozen solutions containing a basic amino acid (e.g., L-arginine, L-lysine, L-histidine) and a hydroxy di- or tricarboxylic acid (e.g., citric acid, L-tartaric acid, DL-malic acid) showed glass transition of maximally freeze-concentrated solute at temperatures (T'g) significantly higher than those of the individual solute solutions. Mixing of the amino acid with some dicarboxylic acids (e.g., oxalic acid) also suggested an upward shift of the transition temperature. Contrarily, combinations of the amino acid with monocarboxylic acids (e.g., acetic acid) had T'gs between those of the individual solute solutions. Co-lyophilization of the basic amino acids and citric acid or L-tartaric acid resulted in amorphous solids that have glass transition temperatures (Tg) higher than the individual components. Mid- and near-infrared analysis indicated altered environment around the functional groups of the consisting molecules. Some of the glass-state excipient combinations protected an enzyme (lactate dehydrogenase, LDH) from inactivation during freeze-drying. The glass-state excipient combinations formed by hydrogen-bonding and electrostatic interaction network would be potent alternative to stabilize therapeutic proteins in freeze-dried formulations.
Izutsu
Ken-ichi
K
National Institute of Health Sciences, Setagaya, Tokyo, Japan. Izutsu@nihs.go.jp
Kadoya
Saori
S
Yomota
Chikako
C
Kawanishi
Toru
T
Yonemochi
Etsuo
E
Terada
Katsuhide
K
D024361:Amino Acids, Basic / Q000737:chemistry*; D003998:Dicarboxylic Acids / Q000737:chemistry*; D005612:Freeze Drying; D005898:Glass; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D024361:Amino Acids, Basic; D003998:Dicarboxylic Acids; D011506:Proteins; D007770:L-Lactate Dehydrogenase