Reconstruction of calvarial defect using a tricalcium phosphate-oligomeric proanthocyanidins cross-linked gelatin composite.
A biodegradable GTP composite which was composed of oligomeric proanthocyanidins (OPCs) cross-linked gelatin mixed with tricalcium phosphate was developed as a bone substitute. The subcutaneous implantation in rats was examined to determine the in vivo degradation and biocompatibility of the GTP composites with various cross-linking densities. Experimental results indicated that the rate of in vivo degradation was markedly attenuated as the concentration of OPCs increased above 5.0wt%. Furthermore, this study examined the biological response of rabbit calvarial bone to GTP composite to evaluate its potential for use as an osteoconductive bone substitute. Bone defects (10mm in diameter) in New Zealand white rabbits were filled with the GTP composite. The de-protenized bovine cancellous bone matrix was employed as the control material. The results of radiographic analyses demonstrated obviously greater new bone ingrowth in the GTP composite than in the de-proteinized bovine bone at the same implantation time. Progressive replacement of the GTP composite by new bone proceeded by a combination of osteoconduction and biodegradation. The biodegradable GTP composite thus has great potential for improving bone repair.
Chen
Kuo-Yu
KY
Institute of Biomedical Engineering and Material Science, Central Taiwan University of Science and Technology, Taichung, Taiwan.
Shyu
Pei-Chi
PC
Dong
Guo-Chung
GC
Chen
Yueh-Sheng
YS
Kuo
Wei-Wen
WW
Yao
Chun-Hsu
CH
D000818:Animals; D001672:Biocompatible Materials; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D020007:Implants, Experimental; D008297:Male; D044945:Proanthocyanidins / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D044945:Proanthocyanidins; D005780:Gelatin; C018392:tricalcium phosphate