Culture-independent analysis of microbial succession during composting of Swine slurry and mushroom cultural wastes.
Bacterial diversity and the composition of individual communities during the composting process of swine and mushroom cultural wastes in a field-scale composter (Hazaka system) were examined using a PCR-based approach. The composting process was divided into six stages based on recorded temperature changes. Phylogenetic analysis of eighty 16S rRNA sequences from uncultured composting bacterial groups revealed the presence of representatives from three divisions, including plant pathogenic bacteria, high-molecule-degrading bacteria and spore-forming bacteria. The plant pathogen A. tumefaciens gradually decreased in abundance during the composting process and eventually disappeared during the thermophilic and cooling stage. A bacterium homologous to Bacillus humi first appeared at the early thermophilic stage and was established at the intermediate thermophilic, post-thermophilic, and cooling stages. It was not possible to isolate the B. humi during any of the stages using general culture techniques.
Cho
Kye Man
KM
Research Institute of Agriculture and Life Science,Gyeongsang National University, Chinju 660-701, Korea.
Lee
Sun Mi
SM
Math
Renukaradhya K
RK
Islam
Shah Md Asraful
SM
Kambiranda
Devaiah M
DM
Kim
Jong Min
JM
Yun
Myoung Geun
MG
Cho
Ji Joong
JJ
Kim
Jong Ok
JO
Lee
Young Han
YH
Kim
Hoon
H
Yun
Han Dae
HD
D000363:Agaricales; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D044822:Biodiversity; D015169:Colony Count, Microbial; D008372:Manure / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013552:Swine; D013696:Temperature
D008372:Manure; D012336:RNA, Ribosomal, 16S