title: A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells.
abstract: Insect cell expression systems are widely used to produce active recombinant proteins. Here, we have developed a high-level expression vector containing a selectable marker for continuous production of recombinant proteins in insect cells. The plasmid, pXIHAbla, developed in this study, established a polyclonal cell line 8 days shorter than pXINSECT-DEST38 and pBmAneo. In addition, pXIHAbla exhibited an approximately fivefold higher average enhanced GFP expression level and approximately a twofold higher bionanocapsule secretion level than pXINSECT-DEST38. Using this plasmid, insect cells that highly express active proteins have been easily established.
authors.affiliation: Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada-Ku, Kobe, 657-8501, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Shishido
authors.forename: Naoya
authors.initials: N
authors.lastname: Kurata
authors.forename: Myung Eui
authors.initials: ME
authors.lastname: Yoon
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamaji
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D004351:Drug Resistance; D015870:Gene Expression; D005822:Genetic Vectors; D007313:Insecta; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012641:Selection, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins