Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus.
Although Sulfolobus species are among the best studied archaeal micro-organisms, the development and availability of genetic tools has lagged behind. In the present paper, we discuss the latest progress in understanding recombination events of exogenous DNA into the chromosomes of Sulfolobus solfataricus and Sulfolobus acidocaldarius and their application in the construction of targeted-deletion mutant strains.
Wagner
Michaela
M
Max-Planck-Institute for Terrestrial Microbiology, Karl-von Frisch-Strasse 1, 35043 Marburg, Germany.
Berkner
Silvia
S
Ajon
Malgorzata
M
Driessen
Arnold J M
AJ
Lipps
Georg
G
Albers
Sonja-Verena
SV
D019847:Chromosomes, Archaeal / Q000235:genetics; D003434:Crossing Over, Genetic; D019641:DNA, Archaeal / Q000235:genetics; D017353:Gene Deletion; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D016944:Sulfolobus / Q000235:genetics*
D019641:DNA, Archaeal