Positive effects of Taxol pretreatment on morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrification of in vitro matured porcine oocytes.
This study was designed to determine the effects of Taxol pretreatment on the morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrified porcine oocytes matured in vitro. The result showed that: (1) the rate of normal mitochondria distribution in fresh group (92.85%) was significantly higher (P<0.05) than that in other three groups (toxicity, 72.48%; vitrification, 50.83%; Taxol+vitrification, 69.98%) and Taxol pretreatment significantly (P<0.05) increased the ratio of normal mitochondria distribution in vitrified oocytes; (2) lipid droplets in vitrified oocytes got cracked, resulting in a great number of smaller lipid droplets (diameter <5 microm). The number of lipid droplets (5-10 microm in diameter) in vitrified oocytes pretreated with Taxol was higher (P<0.05) than that in the oocytes without Taxol pretreatment (81.87+/-13.63 vs. 64.27+/-13.72); (3) both toxicity and vitrification cause the difference in the ultrastructure of mitochondria and lipid droplets. Mitochondria were well maintained in the form of typical round and ellipse shape with smooth surface and clear outline and lipid droplets existed in the form of integrity in Taxol pretreatment group. In conclusion, Taxol pretreatment has positive effects on vitrified porcine oocytes matured in vitro in terms of morphology, distribution and ultrastructure of mitochondria and lipid droplets.
Fu
Xiang-Wei
XW
College of Animal Science and State Key Laboratories for Agrobiotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, PR China.
Shi
Wen-Qing
WQ
Zhang
Qing-Jin
QJ
Zhao
Xue-Ming
XM
Yan
Chang Liang
CL
Hou
Yun-Peng
YP
Zhou
Guang-Bin
GB
Fan
Zhi-Qiang
ZQ
Suo
Lun
L
Wusiman
Abuliz
A
Wang
Yan-Ping
YP
Zhu
Shi-En
SE
D000003:Abattoirs; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D054885:Cumulus Cells / Q000166:cytology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D005260:Female; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure*; D054315:Oocyte Retrieval / Q000379:methods / Q000662:veterinary; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D010053:Ovary / Q000166:cytology; D017239:Paclitaxel / Q000494:pharmacology*; D013552:Swine
D019855:Ethylene Glycol; D017239:Paclitaxel; D004121:Dimethyl Sulfoxide