Label-free DNA biosensors based on functionalized carbon nanotube field effect transistors.
A carbon nanotube transistor array was used to detect DNA hybridization. A new approach to ensure specific adsorption of DNA to the nanotubes was developed. The polymer poly (methylmethacrylate(0.6)-co-poly(ethyleneglycol)methacrylate(0.15)-co-N-succinimidyl methacrylate(0.25)) was synthesized and bonded noncovalently to the nanotube. Aminated single-strand DNA was then attached covalently to the polymer. After hybridization, statistically significant changes were observed in key transistor parameters. Hybridized DNA traps both electrons and holes, possibly caused by the charge-trapping nature of the base pairs.
Martinez
Maria Teresa
MT
Instituto de Carboquímica, Miguel Luesma, 4 Zaragoza, Spain.
Tseng
Yu-Chih
YC
Ormategui
Nerea
N
Loinaz
Iraida
I
Eritja
Ramon
R
Bokor
Jeffrey
J
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes / Q000032:analysis* / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D014173:Transistors, Electronic
D015342:DNA Probes; D037742:Nanotubes, Carbon; D004247:DNA