title: Stepwise esterification of phytosterols with conjugated linoleic acid catalyzed by Candida rugosa lipase in solvent-free medium.
abstract: We conducted a near quantitative esterification of phytosterols from soybean oil deodorizer distillate with conjugated linoleic acid. We used a 1:1 molar ratio of sterols to conjugated linoleic acid. For that matter, stepwise addition of sterols was investigated. Total sterols were divided into several portions and added sequentially to the reaction mixture. Using this methodology, purities of up to 80% steryl esters were obtained that consumed more than 90% of the total conjugated linoleic acid. In addition, the effects of temperature, amount, and stability of lipase were also evaluated.
authors.affiliation: Sección Departamental de Ciencias de la Alimentación, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain. carlos.torres@uam.es
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Torres
authors.forename: Guzman
authors.initials: G
authors.lastname: Torrelo
authors.forename: Luis
authors.initials: L
authors.lastname: Vazquez
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Señorans
authors.forename: Guillermo
authors.initials: G
authors.lastname: Reglero
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004951:Esterification; D044243:Linoleic Acids, Conjugated / Q000737:chemistry* / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D010840:Phytosterols / Q000737:chemistry* / Q000378:metabolism; D012997:Solvents
chemical_list: D044243:Linoleic Acids, Conjugated; D010840:Phytosterols; D012997:Solvents; D008049:Lipase