title: Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite.
abstract: This study introduces the application of calcium-derivatized hydroxyapatite for purification of Fab. Fab binds to native hydroxyapatite but fails to bind to the calcium derivatized form. IgG, Fc, and most other protein contaminants bind to the calcium form. This supports Fab purification by a simple flow-through method that achieves greater than 95% purity from papain digests and mammalian cell culture supernatants. Alternatively, Fab can be concentrated on native hydroxyapatite then eluted selectively by conversion to the calcium-derivatized form.
authors.affiliation: Validated Biosystems, San Clemente, CA 92672, USA. pete_gagnon@mac.com
authors.forename: Pete
authors.initials: P
authors.lastname: Gagnon
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Cheung
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Yazaki
mesh_terms: D002118:Calcium / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D003470:Culture Media; D017886:Durapatite / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D017886:Durapatite; D002118:Calcium