[Estimation of abundance dynamics of gram-negative bacteria in soil].
Bacterial succession in soil was studied for two variants of initiation (moistening and moistening with addition of glucose). To determine the numbers of viable gram-negative bacteria, the modified nalidixic acid method was applied. The numbers of gram-negative bacteria revealed by this method were 2 to 3.5 times higher than those determined by the traditional method. In a developing community, the highest total bacterial numbers were observed on day 7; afterwards their numbers decreased and stabilized at a level exceeding four- to fivefold the initial one. In both experimental variants, the highest numbers of viable gram-negative bacteria were revealed on day 15 (75-85% of the total bacterial numbers). Morphology of these bacteria suggests their classification as cytophagas (chitinophagas) utilizing chitin from the dead fungal mycelium.
Polianskaia
L M
LM
Ivanov
K E
KE
Guzev
V S
VS
Zviagintsev
D G
DG
D002686:Chitin / Q000378:metabolism; D015169:Colony Count, Microbial / Q000379:methods; D006090:Gram-Negative Bacteria / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D009268:Nalidixic Acid; D012426:Russia; D012988:Soil Microbiology; D013997:Time Factors
D002686:Chitin; D009268:Nalidixic Acid