Comparative proteomics of leaf, stem, and root tissues of synthetic Brassica napus.
Comparative proteomics was applied to three vegetative organs of Brassica napus, the leaf, stem, and root using 2-DE. Among the >1600 analyzed spots, 43% were found to be common to all three organs, suggesting the existence of a "basal" or ubiquitous proteome composed of housekeeping proteins. The green organs, leaf, and stem, were closely related (approximately 80% common spots) while the root displayed more organ-specific polypeptides (approximately 10%). Reference maps were established using MS, allowing the identification of 93, 385, and 266 proteins in leaf, stem, and root proteomes, respectively. Bioinformatic analyses were also performed; in silico functional categorization and cellular localization allow obtaining a precise picture of the cell molecular network within vegetative organs. These proteome maps can be explored using the PROTICdb software at the following address: http://bioinformatique.moulon.inra.fr/proticdb/web_view/.
Albertin
Warren
W
Team Evolutionary Genetics: Adaptation and Redundancy, UMR 0320/UMR 8120 Génétique Végétale, INRA, Univ Paris-Sud, CNRS, AgroParisTech, Ferme du Moulon, Gif-sur-Yvette, France. albertin@moulon.inra.fr
Langella
Olivier
O
Joets
Johann
J
Négroni
Luc
L
Zivy
Michel
M
Damerval
Catherine
C
Thiellement
Hervé
H
D029688:Brassica napus / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*