title: Effects of full-length borealin on the composition and protein-protein interaction activity of a binary chromosomal passenger complex.
abstract: The chromosomal passenger complex (CPC) comprises at least four protein components and functions at various cellular localizations during different mitotic stages to ensure correct chromosome segregation and completion of cytokinesis. Borealin, the most recently identified member of the CPC, is an intrinsically unstructured protein of low solubility and stability. Recent reports have demonstrated the formation of binary or ternary CPC subcomplexes incorporating short Borealin fragments in vitro. Using isothermal titration calorimetry, we show that full-length Borealin, instead of a Borealin fragment possessing the complete Survivin and INCENP recognition sequence, is required for the composition of a Borealin-Survivin complex competent to interact with INCENP. In addition, we show evidence that full-length Borealin, which forms high-order oligomers in its isolated form, is a monomer in the Borealin-Survivin CPC subcomplex.
authors.affiliation: Institute for Structural and Molecular Biology, Division of Biosciences, University College London, UK.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhou
authors.forename: Jiejin
authors.initials: J
authors.lastname: Li
authors.forename: Roger
authors.initials: R
authors.lastname: George
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ruchaud
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Zhou
authors.forename: John E
authors.initials: JE
authors.lastname: Ladbury
authors.forename: William C
authors.initials: WC
authors.lastname: Earnshaw
authors.forename: Xuemei
authors.initials: X
authors.lastname: Yuan
mesh_terms: D002151:Calorimetry; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D002850:Chromatography, Gel; D002875:Chromosomes / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D014461:Ultracentrifugation
chemical_list: D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes