Toxicity of lindane (gamma-hexachloroxiclohexane) in Sparus aurata, Crassostrea angulata and Scrobicularia plana.
The purpose of the present study was to research the sublethal and/or lethal effects produced by the exposure of fish and shellfish to the gamma isomer of lindane, gamma-hexachlorocyclohexane (gamma-HCH). The teleostean fish Sparus aurata and the shellfish Crassostrea angulata and Scrobicularia plana, were exposed to 16 micro g/L of lindane for 15 days. Samples of different fish (liver, kidney and gills) and shellfish (gills, gut, digestive gland and mantle) tissues were extracted and processed for histopathological observations. Although mortality was not detected during the bioassay, sublethal effects (histopathological alterations) were observed. Vacuolization in the liver cells and lamellar fusion in gills from exposed fish were observed. Disorganization of normal gill structure, epithelial desquamation with the disappearance of apical ciliature in intestine, and inflammatory response in mantle from exposed shellfish were also observed. Thus, it can be concluded that the lindane concentration employed in the present research did not produce lethal effects in the exposed organisms but it caused sublethal effects. Lindane has time-dependent multiple toxic effects in S. aurata, C. angulata and S. plana, which were more severe at the end of the experimental time. The toxicological implications arising from these results are subjects for further multiconcentration tests dealing with lethal responses (mortality) or with sublethal responses (cellular/molecular biomarkers) of the aforementioned species.
Gonzalez De Canales
Maria Luisa
ML
Biology Department, Marine and Environmental Sciences Faculty, University of Cadiz, Cadiz, Spain.
Oliva
Milagrosa
M
Garrido
Carmen
C
D000818:Animals; D001681:Biological Assay; D049896:Crassostrea / Q000187:drug effects*; D001556:Hexachlorocyclohexane / Q000493:pharmacokinetics / Q000633:toxicity*; D007150:Immunohistochemistry; D007306:Insecticides / Q000493:pharmacokinetics / Q000633:toxicity*; D021541:Sea Bream / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
D007306:Insecticides; D014874:Water Pollutants, Chemical; D001556:Hexachlorocyclohexane