DNA-induced aggregation and fusion of phosphatidylcholine liposomes in the presence of multivalent cations observed by the cryo-TEM technique.
By means of cryoelectron transmission microscopy (cryo-TEM), we were able to demonstrate the formation of ternary complexes (TC): DNA-phosphatidylcholine liposome-divalent metal cations. Addition of Ba2+ to TC led to visualization of DNA compacting on the liposome surface. Staining the TC by Tb3+ cations revealed the changed secondary structure of DNA located between fused liposomes. Cryo-TEM and liposome turbidity data were analyzed during TC formation. Liposome aggregation and the liposome fusion induced by DNA in TC were observed. Because TC displayed the property of DNA cationic liposome complexes as well as their own unique properties, we were able to consider cationic lipoplexes as a particular case of TC. The involvement of TC and direct DNA-lipid interactions in the formation nuclear pore complexes were assumed.
Kuvichkin
Vasily V
VV
Department of Mechanisms Reception of the Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Reg., Russia. vvkuvichkin@gmail.com
Danev
Radostin S
RS
Shigematsu
Hideki
H
Nagayama
Kuniaki
K
D000818:Animals; D001464:Barium / Q000737:chemistry*; D002412:Cations; D002417:Cattle; D020285:Cryoelectron Microscopy; D004247:DNA / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009690:Nucleic Acid Conformation; D010713:Phosphatidylcholines / Q000737:chemistry*; D013725:Terbium / Q000737:chemistry*
D002412:Cations; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D013725:Terbium; D001464:Barium; D004247:DNA; C427203:calf thymus DNA