title: Epigenetic profiling reveals etiologically distinct patterns of DNA methylation in head and neck squamous cell carcinoma.
abstract: Head and neck squamous cell carcinomas (HNSCCs) represent clinically and etiologically heterogeneous tumors affecting >40 000 patients per year in the USA. Previous research has identified individual epigenetic alterations and, in some cases, the relationship of these alterations with carcinogen exposure or patient outcomes, suggesting that specific exposures give rise to specific types of molecular alterations in HNSCCs. Here, we describe how different etiologic factors are reflected in the molecular character and clinical outcome of these tumors. In a case series of primary, incident HNSCC (n = 68), we examined the DNA methylation profile of 1413 autosomal CpG loci in 773 genes, in relation to exposures and etiologic factors. The overall pattern of epigenetic alteration could significantly distinguish tumor from normal head and neck epithelial tissues (P < 0.0001) more effectively than specific gene methylation events. Among tumors, there were significant associations between specific DNA methylation profile classes and tobacco smoking and alcohol exposures. Although there was a significant association between methylation profile and tumor stage (P < 0.01), we did not observe an association between these profiles and overall patient survival after adjustment for stage; although methylation of a number of specific loci falling in different cellular pathways was associated with overall patient survival. We found that the etiologic heterogeneity of HNSCC is reflected in specific patterns of molecular epigenetic alterations within the tumors and that the DNA methylation profiles may hold clinical promise worthy of further study.
authors.affiliation: Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02912, USA.
authors.forename: Carmen J
authors.initials: CJ
authors.lastname: Marsit
authors.forename: Brock C
authors.initials: BC
authors.lastname: Christensen
authors.forename: E Andres
authors.initials: EA
authors.lastname: Houseman
authors.forename: Margaret R
authors.initials: MR
authors.lastname: Karagas
authors.forename: Margaret R
authors.initials: MR
authors.lastname: Wrensch
authors.forename: Ru-Fang
authors.initials: RF
authors.lastname: Yeh
authors.forename: Heather H
authors.initials: HH
authors.lastname: Nelson
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Wiemels
authors.forename: Shichun
authors.initials: S
authors.lastname: Zheng
authors.forename: Marshall R
authors.initials: MR
authors.lastname: Posner
authors.forename: Michael D
authors.initials: MD
authors.lastname: McClean
authors.forename: John K
authors.initials: JK
authors.lastname: Wiencke
authors.forename: Karl T
authors.initials: KT
authors.lastname: Kelsey
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D018899:CpG Islands; D019175:DNA Methylation / Q000235:genetics / Q000502:physiology*; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006258:Head and Neck Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects
chemical_list: D014408:Biomarkers, Tumor