Application of atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry for rapid identification of Neisseria species.
Atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI MS) was applied to develop a proteomics-based method to detect and identify Neisseria species. Heat-inactivated clinical isolate cell suspensions of Neisseria gonorrhoeae and strains belonging to five serogroups (A, B, C, W135, and Y) of Neisseria meningitidis were subjected to on-probe protein/peptide extraction and tryptic digestion followed by AP-MALDI tandem MS (MS/MS)-based proteomic analysis. Amino acid sequences derived from three protonated peptides with m/z values of 1743.8, 1894.8, and 1946.8 were identified by AP-MALDI MS/MS and MASCOT proteome database search analysis as belonging to neisserial acyl carrier protein, neisserial-conserved hypothetical protein, and neisserial putative DNA binding protein, respectively. These three peptide masses can thus be potential biomarkers for neisserial species identification by AP-MALDI MS.
Gudlavalleti
Seshu K
SK
Science and Engineering Services, Inc, Columbia, MD 21046, USA. seshu@apmaldi.com
Sundaram
Appavu K
AK
Razumovski
Jane
J
Doroshenko
Vladimir
V
D000595:Amino Acid Sequence; D001274:Atmospheric Pressure; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001709:Biotechnology; D006801:Humans; D008969:Molecular Sequence Data; D009343:Neisseria / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009345:Neisseria meningitidis / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010446:Peptide Fragments / Q000235:genetics / Q000302:isolation & purification; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods; D012703:Serotyping; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014357:Trypsin
D001426:Bacterial Proteins; D010446:Peptide Fragments; D020543:Proteome; D014357:Trypsin