Delayed sodium thiosulphate administration reduces cisplatin efficacy on mouse EMT6 tumour cells in vitro.
OBJECTIVE
To determine whether simultaneous and/or delayed administration of sodium thiosulphate (STS) affects the oncologic effect of cisplatin or cisdiaminedichloroplatinum (CDDP) in EMT6 tumour cells in vitro.


METHODS
Cell biology research laboratory.


METHODS
Clonogenic assays of EMT6 tumour cells with CDDP alone, CDDP plus simultaneous STS, and CDDP plus a 4-hour delay of STS were performed. Growth fractions under these three conditions were compared.


RESULTS
Tumour growth was statistically significantly increased when CDDP and STS were administered compared with CDDP alone. There was no statistically significant difference between simultaneous and 4-hour delay of STS administration. We conclude that in EMT6 cells, either simultaneous administration or a 4-hour delay of STS administration significantly decreases CDDP efficacy.


CONCLUSIONS
STS adversely affects CDDP's oncologic efficacy in EMT6 cell cultures in vitro.
Yee
May-Sann
MS
Department of Anesthesia and Pain Medicine, University of Alberta, Edmonton, Alberta.
Blakley
Brian W
BW
Begleiter
Asher
A
Leith
Marsha
M
D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000009:adverse effects* / Q000494:pharmacology*; D004334:Drug Administration Schedule; D004347:Drug Interactions; D064420:Drug-Related Side Effects and Adverse Reactions / Q000517:prevention & control*; D006207:Half-Life; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D011336:Probability; D012680:Sensitivity and Specificity; D013885:Thiosulfates / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D013885:Thiosulfates; C017717:sodium thiosulfate; D002945:Cisplatin