title: [Characterization and expression profiling of a novel cereal cyst nematode resistance gene analog in wheat].
abstract: Based on the conserved regions of known resistance genes, a NBS-LRR type CCN resistance gene analog was isolated from the CCN resistant E-10 near isogenic lines (NILs) of wheat, designated as CreZ (GenBank Acc. N: EU327996). It contained a complete ORF that was 2775 bp in length and encoded 924 amino acids. Sequence comparison indicated that it shared 92% nucleotide and 87% amino acid identity with those of the known CCN-resistance gene Cre3 and had similar characteristic conserved motifs as those in other established NBS-LRR disease resistance genes. The expression profiling of CreZ indicated that it was specifically expressed in the roots of resistant plants and real-time PCR analysis demonstrated that expression levels drastically increased when the plants were inoculated with cereal cyst nematodes. It could be inferred, then, that CreZ belongs to the NBS-LRR resistance gene family and is a candidate gene for potential resistance to the cereal cyst nematode.
authors.forename: X G
authors.initials: XG
authors.lastname: Zhai
authors.forename: T
authors.initials: T
authors.lastname: Zhao
authors.forename: Y H
authors.initials: YH
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Long
authors.forename: G B
authors.initials: GB
authors.lastname: Deng
authors.forename: Z F
authors.initials: ZF
authors.lastname: Pan
authors.forename: M Q
authors.initials: MQ
authors.lastname: Yu
mesh_terms: D000818:Animals; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D009348:Nematoda; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D018515:Plant Leaves / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology*
chemical_list: D010940:Plant Proteins