Silencing of mitochondrial alternative oxidase gene of Aspergillus fumigatus enhances reactive oxygen species production and killing of the fungus by macrophages.
We previously demonstrated that conidia from Aspergillus fumigatus incubated with menadione and paraquat increases activity and expression of cyanide-insensitive alternative oxidase (AOX). Here, we employed the RNA silencing technique in A. fumigatus using the vector pALB1/aoxAf in order to down-regulate the aox gene. Positive transformants for aox gene silencing of A. fumigatus were more susceptible both to an imposed in vitro oxidative stress condition and to macrophages killing, suggesting that AOX is required for the A. fumigatus pathogenicity, mainly for the survival of the fungus conidia during host infection and resistance to reactive oxygen species generated by macrophages.
Magnani
Taisa
T
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil.
Soriani
Frederico Marianetti
FM
Martins
Vicente de Paulo
Vde P
Policarpo
Anna Carolina de Freitas
AC
Sorgi
Carlos Artério
CA
Faccioli
Lúcia Helena
LH
Curti
Carlos
C
Uyemura
Sérgio Akira
SA
D017209:Apoptosis / Q000502:physiology*; D001232:Aspergillus fumigatus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D020868:Gene Silencing; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D008928:Mitochondria / Q000201:enzymology*; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013172:Spores, Fungal; D000099188:Alternative Oxidase
D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D000099188:Alternative Oxidase