Altered P-selectin and CD44 expression in the renal tissues and peripheral blood of children with IgA nephropathy.
OBJECTIVE
To understand the role of P-selectin (CD62P) and CD44 in mediating immune inflammation in the nephrotic process of children with IgA nephropathy (IgAN), cooperative expression of CD62P and CD44 in peripheral blood and renal tissues of IgAN children was investigated and its association with changes of histopathologic, serologic, and urinary properties was tested.


METHODS
Forty-six IgAN children were divided into three groups according to pathologic grades and clinical features. Fifteen blood samples from normal children and four normal renal biopsy specimens were used as controls. Plasma level of CD62P was detected by double antibody sandwich immunoradiometric assay; ELISA was used to determine serum level of CD44. Expression of CD62P and CD44 in renal tissues was determined by immunohistochemistry.


RESULTS
Cooperative expression of CD62P and CD44 was detected in renal tissues and peripheral blood of IgAN children. Altered expression of CD62P and CD44 in peripheral blood significantly correlated not only with hematuria, proteinuria, serum cholesterol, and albumin, and with urine NAG and beta(2)-MG, but also with degree of tubulointerstitial injury in IgAN children.


CONCLUSIONS
The evidence supported CD62P and CD44 as initial and promoting factors mediating immune inflammation in the nephrotic process in IgAN children. The cooperative expression profiles of CD62P and CD44 in renal tissues and peripheral blood combined with serologic and urinary predictors may be important in diagnosis of progression in children with IgAN.
Qiaoling
Zhang
Z
Department of Pediatrics, Huang pu Division of The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Xiaoyun
Jiang
J
Wei
Wu
W
Shuhong
Dong
D
Yaqin
Peng
P
Xiaoqing
Guan
G
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005922:Glomerulonephritis, IGA / Q000097:blood* / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D019007:P-Selectin / Q000096:biosynthesis*
C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D019007:P-Selectin