title: In vivo osteogenic differentiation of human adipose-derived stem cells in an injectable in situ-forming gel scaffold.
abstract: The sol-to-gel transition occurring at around body temperature makes the MPEG-PCL diblock copolymer an ideal candidate material for use as an injectable in situ-forming gel containing human adipose tissue-derived stem cells (hADSCs). The sol can be prepared at room temperature, and the gel forms at body temperature. Solutions of the copolymer containing hADSCs and osteogenic factors injected into rats formed gel scaffolds at the injection sites. The gels thus formed showed the interconnective pore structure required to support growth, proliferation, and differentiation of hADSCs. Bromodeoxyuridine-labeled hADSCs were confirmed to be present in gels formed in vivo. Bone formation was observed only in gel implants containing both hADSCs and osteogenic factors. Subcutaneous implantation of the in situ-forming gel scaffold demonstrated that hADSCs embedded in the gel stimulated much lower host tissue responses than did the gel alone, probably because of the unique immunomodulatory properties of hADSCs. In conclusion, our data on hADSCs embedded in an in situ gel scaffold suggest that this formulation may provide numerous benefits as a noninvasive alternative for tissue-engineered bone formation.
authors.affiliation: Fusion Biotechnology Research Center, Korea Research Institute of Chemical Technology, Daejeon, Korea.
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Ahn
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Kim
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Il Woo
authors.initials: IW
authors.lastname: Lee
authors.forename: Heung Jae
authors.initials: HJ
authors.lastname: Chun
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Hai Bang
authors.initials: HB
authors.lastname: Lee
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D005260:Female; D005455:Fluorescent Antibody Technique; D005782:Gels; D006801:Humans; D007249:Inflammation; D007267:Injections; D007279:Injections, Subcutaneous; D010012:Osteogenesis; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D013696:Temperature; D054457:Tissue Scaffolds; D014783:Viscosity
chemical_list: D005782:Gels; D001973:Bromodeoxyuridine