Label-free detection of drug-membrane association using ultraviolet-visible sum-frequency generation.
Drug-membrane interactions play a crucial role in the pharmacology and activity of drugs. The measurement of drug association to lipid membranes has conventionally been measured by fluorescence and other spectroscopic methods. However, a main disadvantage of fluorescence labeling of drugs is that the introduction of fluorophores may change the molecules physical properties, such as charge, hydrophobic or hydrophilic character, and structure. To circumvent these problems, Ultraviolet-Visible Sum Frequency Generation (UV-Vis SFG) has been developed as an ultrasensitive and label-free technique to detect small-molecule drug association to lipid membranes. Four different classes of drugs, a nonsteroidal anti-inflammatory drug (ibuprofen), antibiotic (azithromycin), antifungal (tolnaftate), and local anesthetic (tetracaine), were examined. Drug association was measured on planar supported lipid bilayers (PSLBs) of 1,2-dioleoyl-sn-glycero-3-phophocholine (DOPC). Equilibrium association constants of the drugs were obtained and correlate well to the partition coefficients of the drugs in a liposome membrane-water system. UV-Vis SFG is a powerful and novel technique to directly measure the association of drugs to a single biological membrane without chemical modification.
Nguyen
Trang T
TT
Department of Chemistry, University of Utah, 315 S. 1400 E. RM. 2020, Salt Lake City, Utah 84112, USA.
Rembert
Kelvin
K
Conboy
John C
JC
D008051:Lipid Bilayers / Q000737:chemistry*; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*
D008051:Lipid Bilayers; D004364:Pharmaceutical Preparations