Proteomic analysis of oil mobilization in seed germination and postgermination development of Jatropha curcas.
To understand oil mobilization in germinating seeds, we performed ultrastructural observation and proteomic analysis of endosperm in germinating Jatropha curcas seeds. Results showed that the oil mobilization was initiated during germination, and then the oil was consumed for early seedling development. The significant change in abundance of 50 protein spots during germination indicated that several pathways including beta-oxidation, glyoxylate cycle, glycolysis, citric acid cycle, gluconeogenesis, and pentose phosphate pathway were involved in the oil mobilization.
Yang
Ming-Feng
MF
Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, P. R. China and Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100080, P. R. China.
Liu
Yu-Jun
YJ
Liu
Yun
Y
Chen
Hui
H
Chen
Fan
F
Shen
Shi-Hua
SH
D000595:Amino Acid Sequence; D018525:Germination / Q000502:physiology*; D031286:Jatropha / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism* / Q000648:ultrastructure
D010938:Plant Oils; D010940:Plant Proteins; D020543:Proteome