Time course of islet loss after intraportal transplantation.
A high percentage of islets fail to survive intraportal transplantation, but the absolute amount and time course of this loss are uncertain. We have devised a technique to directly quantitate the number of surviving islets using simultaneous selective transplantation of islets and inert beads into the posterior lobes of the liver. Islet:bead ratio did not change significantly within the first 2 hours, but fell progressively thereafter, giving calculated islet survival rates of 89, 43, and 66% at days 1, 7 and 28, respectively. This technique provides a baseline from which to develop strategies to improve the survival of intraportally transplanted islets.
Pawelec
Krystian
K
Royal Free and University College Medical School, University College London, London, England, UK. krystian.pawelec@yahoo.com
Juszczak
Maciej Tadeusz
MT
Kumar
Anila
A
Powis
Steve Hugh
SH
Press
Martin
M
D000818:Animals; D002452:Cell Count; D006084:Graft Rejection / Q000209:etiology; D006085:Graft Survival / Q000502:physiology*; D007275:Injections, Intravenous; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008863:Microspheres; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors