Effects of full-length borealin on the composition and protein-protein interaction activity of a binary chromosomal passenger complex.
The chromosomal passenger complex (CPC) comprises at least four protein components and functions at various cellular localizations during different mitotic stages to ensure correct chromosome segregation and completion of cytokinesis. Borealin, the most recently identified member of the CPC, is an intrinsically unstructured protein of low solubility and stability. Recent reports have demonstrated the formation of binary or ternary CPC subcomplexes incorporating short Borealin fragments in vitro. Using isothermal titration calorimetry, we show that full-length Borealin, instead of a Borealin fragment possessing the complete Survivin and INCENP recognition sequence, is required for the composition of a Borealin-Survivin complex competent to interact with INCENP. In addition, we show evidence that full-length Borealin, which forms high-order oligomers in its isolated form, is a monomer in the Borealin-Survivin CPC subcomplex.
Zhou
Lihong
L
Institute for Structural and Molecular Biology, Division of Biosciences, University College London, UK.
Li
Jiejin
J
George
Roger
R
Ruchaud
Sandrine
S
Zhou
Hong-Gang
HG
Ladbury
John E
JE
Earnshaw
William C
WC
Yuan
Xuemei
X
D002151:Calorimetry; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D002850:Chromatography, Gel; D002875:Chromosomes / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D014461:Ultracentrifugation
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes