title: Immunogenicity of newly constructed attenuated vaccinia strain LC16m8Delta that expresses SIV Gag protein.
abstract: We developed the method to efficiently construct recombinant vaccinia viruses based on LC16m8Delta strain that can replicate in mammalian cells but is still safe in human. Immunization in a prime-boost strategy using DNA and LC16m8Delta expressing SIV Gag elicited 7-30-fold more IFN-gamma-producing T cells in mice than that using DNA and non-replicating vaccinia DIs recombinant strain. As the previous study on the DNA-prime and recombinant DIs-boost anti-SIV vaccine showed protective efficacy in the macaque model [Someya K, Ami Y, Nakasone T, Izumi Y, Matsuo K, Horibata S, et al. Induction of positive cellular and humoral responses by a prime-boost vaccine encoded with simian immunodeficiency virus gag/pol. J Immunol 2006;176(3):1784-95], LC16m8Delta would have potential as a better recombinant viral vector for HIV vaccine.
authors.affiliation: Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Suzuki
authors.forename: Minoru
authors.initials: M
authors.lastname: Kidokoro
authors.forename: Ismael Ben
authors.initials: IB
authors.lastname: Fofana
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohashi
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Okamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Shida
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D005260:Female; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology; D014615:Vaccinia / Q000235:genetics*
chemical_list: D016915:AIDS Vaccines; D015683:Gene Products, gag; D019444:Vaccines, DNA; D007371:Interferon-gamma