Subcellular localization and expression of bamboo mosaic virus satellite RNA-encoded protein.
The satellite RNA of bamboo mosaic virus (satBaMV) has a single open reading frame encoding a non-structural protein, P20, which facilitates long-distance movement of satBaMV in BaMV and satBaMV co-infected plants. Immunohistochemistry and immunoelectron microscopy revealed that the P20 protein accumulated in the cytoplasm and nuclei in co-infected cells. P20 and the helper virus coat protein (CP) were highly similar in their subcellular localization, except that aggregates of BaMV virions were not labelled with anti-P20 serum. The BaMV CP protein was fairly abundant in mesophyll cells, whilst P20 was more frequently detected in mesophyll cells and vascular tissues. The expression kinetics of the P20 protein was similar to but slightly earlier than that of CP in co-infected Bambusa oldhamii protoplasts and Nicotiana benthamiana leaves. However, satBaMV-encoded protein levels declined rapidly in the late phase of co-infection. During co-infection, in addition to the intact P20, a low-molecular-mass polypeptide of 16 kDa was identified as a P20 C-terminally truncated product; the possible method of generation of the truncated protein is discussed.
Palani
Paramasivan Vijaya
PV
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Chiu
Morgan
M
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Chen
Wei
W
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan 402, ROC.
Wang
Ching-Chi
CC
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Lin
Choy-Chieng
CC
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Hsu
Chuen-Chi
CC
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Cheng
Chi-Ping
CP
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Chen
Chung-Mong
CM
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
Hsu
Yau-Heiu
YH
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan 402, ROC.
Lin
Na-Sheng
NS
Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan 115, ROC.|Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan 402, ROC.|Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan 115, ROC.
D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames; D018515:Plant Leaves / Q000821:virology; D019244:RNA, Satellite / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D031787:Sasa / Q000821:virology; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D019244:RNA, Satellite; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins