Effects on lipid peroxidation and antioxidative enzymes of Euonymus alatus in cultured rat hepatocytes.
The Euonymus alatus (Thunb.) Sieb. has long been used as a crude drug. In this paper, we investigate the effects of E. alatus on cultured hepatocyte cell system and lipid peroxidation in hydrogen peroxide (H(2)O(2)) treatment conditions. The study covers the physiological activity (the antioxidative activity and the nitrite-scavenging effect) of E. alatus. H(2)O(2) that can produce intracellular free radical was used for inducer of the peroxidation of cellular lipids. Treatment of E. alatus attenuated in cell killing enhanced by increasing concentrations of H(2)O(2). The increased malondialdehyde level induced by H(2)O(2) treatment was reduced by pre-treatment of E. alatus. Furthermore, addition of E. alatus in cell culture medium significantly reduced cell killing and content of intracellular antioxidants. Changes in nitrite-scavenging effect of E. alatus at various concentrations (5-25 mg/ml) and various pH levels (pH 1.2, 4.2 and 6.0) were also observed. The present study was also done to investigate the effects of E. alatus on cultured hepatocyte cell system, H(2)O(2)-induced cytotoxicity and antioxidative enzyme activities, including catalase, superoxide dismutase, glutathione peroxidase and glutathione S-transferase in H(2)O(2 )treatment conditions. E. alatus treatment had significant protective or elevating activities on these antioxidative enzyme activities compared to a normal group. The results indicate that E. alatus provides a strong antioxidant protection of cells against H(2)O(2)-induced oxidative stress.
Kim
Kyung-Woon
KW
Molecular and Cellular Glycobiology Unit, Department of Biological Science, SungKyunKwan University, Kyunggi.
Suh
Seok-Jong
SJ
Kim
Jong-Dae
JD
Kim
Soo-Sung
SS
Lee
In-Seon
IS
Kim
June-Ki
JK
Chang
Gyu-Tae
GT
Kim
Dong-Soo
DS
Kim
Cheorl-Ho
CH
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D029756:Euonymus; D016166:Free Radical Scavengers / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation / Q000187:drug effects*; D009573:Nitrites / Q000737:chemistry; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D009573:Nitrites; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase