title: [Five alkaloids from vine stems of Diploclisia affinis].
abstract: OBJECTIVE
  To study alkaloid constituents of Diploclisia affinis.
  
  
  METHODS
  The air-dried vine stems of D. affinis were extracted with 90% EtOH three times at room temperature. The EtOH extract was suspended in H2 O and adjusted to pH 2 with 5% HCl solution. After extracted with petroleum ether and CHCl3 successively, the aqueous fraction was adjusted to pH 9 with 10% NH3 x H2O and extracted with CHCl3 again to afford the total alkaloids fraction. The compounds were isolated through column chromatography from the total alkaloids fraction. Their structures were determined on the basis of spectral analysis (MS, 1H-NMR, 13C-NMR).
  
  
  RESULTS
  Five alkaloids was identified as reticuline (1), asimilobine (2), acutumine (3), dihydroxyprotoberberine (4), stepholidine (5), were isolated from the vine stems of D. affinis.
  
  
  CONCLUSIONS
  All the compounds were obtained from D. affinis for the first time.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
authors.forename: En-Jun
authors.initials: EJ
authors.lastname: Wang
authors.forename: Yun-Bao
authors.initials: YB
authors.lastname: Ma
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Jiang
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Chen
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D001060:Aporphines / Q000737:chemistry; D044182:Benzylisoquinolines / Q000737:chemistry; D001599:Berberine / Q000031:analogs & derivatives / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D029583:Menispermaceae / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D013141:Spiro Compounds / Q000737:chemistry
chemical_list: D000470:Alkaloids; D001060:Aporphines; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D013141:Spiro Compounds; C442344:acutumine; D001599:Berberine; C010513:stepholidine; D000431:Ethanol; C054614:asimilobine; C003298:reticuline