Templated spherical high density lipoprotein nanoparticles.
We report the synthesis of high density lipoprotein (HDL) biomimetic nanoparticles capable of binding cholesterol. These structures use a gold nanoparticle core to template the assembly of a mixed phospholipid layer and the adsorption of apolipoprotein A-I. These synthesized structures have the general size and surface composition of natural HDL and, importantly, bind free cholesterol (K(d) = 4 nM). The determination of the K(d) for these particles, with respect to cholesterol complexation, provides a key starting and comparison point for measuring and evaluating the properties of subsequently developed synthetic versions of HDL.
Thaxton
C Shad
CS
Northwestern University, Feinberg School of Medicine, Department of Urology, 303 East Chicago Avenue, Tarry 16-703, Chicago, Illinois 60611, USA. cthaxton003@md.northwestern.edu
Daniel
Weston L
WL
Giljohann
David A
DA
Thomas
Audrey D
AD
Mirkin
Chad A
CA
D006046:Gold / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
D008075:Lipoproteins, HDL; D014867:Water; D006046:Gold