title: Determination of the henipavirus phosphoprotein gene mRNA editing frequencies and detection of the C, V and W proteins of Nipah virus in virus-infected cells.
abstract: The henipaviruses, Nipah virus (NiV) and Hendra virus (HeV), are highly pathogenic zoonotic paramyxoviruses. Like many other paramyxoviruses, henipaviruses employ a process of co-transcriptional mRNA editing during transcription of the phosphoprotein (P) gene to generate additional mRNAs encoding the V and W proteins. The C protein is translated from the P mRNA, but in an alternate reading frame. Sequence analysis of multiple, cloned mRNAs showed that the mRNA editing frequencies of the P genes of the henipaviruses are higher than those reported for other paramyxoviruses. Antisera to synthetic peptides from the P, V, W and C proteins of NiV were generated to study their expression in infected cells. All proteins were detected in both infected cells and purified virions. In infected cells, the W protein was detected in the nucleus while P, V and C were found in the cytoplasm.
authors.affiliation: The Research Institute at Nationwide Children's Hospital, Center for Vaccines and Immunity, 700 Children's Drive, Columbus, OH 43205, USA.|The Ohio State University, College of Medicine, Department of Pediatrics, Columbus, OH 43205, USA.|Emory University School of Medicine, Department of Microbiology and Immunology, 1510 Clifton Road, Atlanta, GA 30322, USA.|Measles, Mumps, Rubella and Herpesviruses Laboratory Branch, 1600 Clifton Road, MS-C-22, Atlanta, GA 30333, USA.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Lo
authors.affiliation: Measles, Mumps, Rubella and Herpesviruses Laboratory Branch, 1600 Clifton Road, MS-C-22, Atlanta, GA 30333, USA.
authors.forename: Brian H
authors.initials: BH
authors.lastname: Harcourt
authors.affiliation: Commonwealth Scientific Industrial Research Organization, Australian Animal Health Laboratory, 5 Portarlington Road, East Geelong, Victoria, Australia.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Mungall
authors.affiliation: Measles, Mumps, Rubella and Herpesviruses Laboratory Branch, 1600 Clifton Road, MS-C-22, Atlanta, GA 30333, USA.
authors.forename: Azaibi
authors.initials: A
authors.lastname: Tamin
authors.affiliation: The Ohio State University, College of Medicine, Department of Pediatrics, Columbus, OH 43205, USA.|The Research Institute at Nationwide Children's Hospital, Center for Vaccines and Immunity, 700 Children's Drive, Columbus, OH 43205, USA.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Peeples
authors.affiliation: Measles, Mumps, Rubella and Herpesviruses Laboratory Branch, 1600 Clifton Road, MS-C-22, Atlanta, GA 30333, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Bellini
authors.affiliation: Measles, Mumps, Rubella and Herpesviruses Laboratory Branch, 1600 Clifton Road, MS-C-22, Atlanta, GA 30333, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Rota
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005822:Genetic Vectors; D045403:Henipavirus / Q000235:genetics*; D007118:Immunoassay; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D010957:Plasmids; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D014764:Viral Proteins; C110268:nonstructural protein C;nonstructural protein V;phosphoprotein P, Hendra virus