title: Fucoidan inhibits UVB-induced MMP-1 promoter expression and down regulation of type I procollagen synthesis in human skin fibroblasts.
abstract: UVB reduces type I procollagen levels and increases matrix metalloproteinases-1 (MMP-1) levels in human skin and plays a major role in the process of photoaging. We previously reported that fucoidan inhibits UVB-induced MMP-1 expression at the protein and mRNA levels in human skin fibroblasts (HS68). Yet, the effects of fucoidan on UVB-induced MMP-1 promoter activity and type I procollagen have not been investigated. In this study, we assessed the effects of fucoidan on the inhibition of MMP-1 promoter activity and on the increase of type I procollagen synthesis in human skin fibroblasts. Fucoidan treatment significantly inhibited MMP-1 promoter activity compared to UVB irradiation alone. Fucoidan treatment also increased type I procollagen mRNA and protein expression in a dose-dependent manner compared to the control. Our data indicate that fucoidan may prevent UVB-induced MMP-1 expression and inhibit down-regulation of type I procollagen synthesis. We suggest that fucoidan may be a potential therapeutic agent to prevent and treat skin photoaging.
authors.affiliation: Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University, 34 Amnam-dong, Suh-gu, Busan, 602-702, Republic of Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Moon
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Mi Jeong
authors.initials: MJ
authors.lastname: Ku
authors.forename: Byeng Chul
authors.initials: BC
authors.lastname: Yu
authors.forename: Man Joong
authors.initials: MJ
authors.lastname: Jeon
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Stonik
authors.forename: Tatyana N
authors.initials: TN
authors.lastname: Zvyagintseva
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Ermakova
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis*; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D024042:Collagen Type I; D011134:Polysaccharides; D012333:RNA, Messenger; C007789:fucoidan; D020781:Matrix Metalloproteinase 1