Mechanism of dihydrouridine synthase 2 from yeast and the importance of modifications for efficient tRNA reduction.
Dihydrouridine synthases (DUSs) are flavin-dependent enzymes that catalyze site-specific reduction of uracils in tRNAs. The mechanism of DUS 2 from Saccharomyces cerevisiae was studied. Previously published turnover rates for this DUS were very low. Our studies show that the catalytic cycle consists of reductive and oxidative half-reactions. The enzyme is reduced by NADPH rapidly but has a very slow oxidative half-reaction using in vitro transcribed tRNA substrates. Using tRNA(Leu) purified from a DUS 2 knockout strain of yeast we obtained reaction rate enhancements of 600-fold over in vitro transcribed substrates, indicating that other RNA modifications are required for rapid uracil reduction. This demonstrates a previously unknown ordering of modifications and indicates that dihydrouridine formation is a later step in tRNA maturation. We also show that an active site cysteine is important for catalysis, likely in the protonation of uracil during tRNA reduction. Dihydrouridine of modified tRNA from Escherichia coli was also oxidized to uridine showing the reaction to be reversible.
Rider
Lance W
LW
Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109-5606, USA.
Ottosen
Mette B
MB
Gattis
Samuel G
SG
Palfey
Bruce A
BA
D020134:Catalytic Domain; D015394:Molecular Structure; D009249:NADP / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism
D012356:RNA, Transfer, Leu; D029701:Saccharomyces cerevisiae Proteins; D009249:NADP; D014498:Uracil; D010088:Oxidoreductases; C107530:SMM1 protein, S cerevisiae