[Effects of granulosa cells treatments and follicular fluid on cleavage rate and blastocyst rate of bovine oocyte after in vitro fertilization and culture].
Experiments were conducted to study the effects of granulosa cell (GC), follicular fluid and their interaction on cleavage rate and blastocyst rate of Bovine oocytes following in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). A total of 2178 oocytes were used for studies on maturation, fertilization and embryo development. The cleavage rate was affected significantly (P < 0.05) by GC, follicular fluid and the interaction of GC and follicular fluid. The blastocyst rate was affected significantly (P < 0.05) by GC, the blastocyst rate wasn't affected significantly by GC, follicular fluid and the interaction of GC and follicular fluid (P > 0.05). Effects of granulosa cell and follicular fluid and their interaction on the cleavage rate and the blastocyst rate in embryo cultured sort order was GC > follicular fluid > interaction of follicular fluid and GC. It was concluded that follicular fluid and granulosa cell monolayer (GCM) adding in TCM199 better support maturation of oocytes and development of embryos in Bovine. There were no differences in the cleavage rate and blastocyst rate, between the groups granulosa cell monolayer and incubating granulosa cell (which were incubated 10 min in incubator before oocytes being transferred) on co-culture system. Granulosa cell monolayer could be replaced by incubating granulosa cell on coculture system on Bovine oocytes IVM, IVF and IVC.
Deng
Wen
W
College of Animal Science and Technology, Henan University of Sci-Tech, Luoyang, Henan 471003. wen.dkj@163.com
Yu
Xue Li
XL
Pang
You Zhi
YZ
Zan
Lin Sen
LS
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000502:physiology*; D006107:Granulosa Cells / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*