Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice.
Rice stripe virus (RSV) infects rice and causes great yield reduction in some Asian countries. In this study, rice cDNA library was screened by a Gal4-based yeast two-hybrid system using pc4, a putative movement protein of RSV, as the bait. A number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. Two of them were selected and further characterized. The two proteins were a J protein and a small Hsp, respectively. Interactions between Pc4 and the two proteins were confirmed using coimmunoprecipitation. Implications of the findings that pc4 interacted with two chaperone proteins were discussed.
Lu
Lianming
L
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
Du
Zhenguo
Z
Qin
Meiling
M
Wang
Ping
P
Lan
Hanhong
H
Niu
Xiaoqing
X
Jia
Dongsheng
D
Xie
Liyan
L
Lin
Qiying
Q
Xie
Lianhui
L
Wu
Zujian
Z
D000595:Amino Acid Sequence; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism*; D050888:Heat-Shock Proteins, Small / Q000378:metabolism; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D010940:Plant Proteins / Q000378:metabolism*; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016415:Sequence Alignment; D029364:Tenuivirus / Q000502:physiology*; D020798:Two-Hybrid System Techniques
D050956:HSP40 Heat-Shock Proteins; D050888:Heat-Shock Proteins, Small; D010940:Plant Proteins; D053830:Plant Viral Movement Proteins