title: Epstein-Barr virus detection in invasive and pre-invasive lesions of the uterine cervix.
abstract: In the present study, our aim was to investigate whether EBV DNA could be found in association with invasive and pre-invasive cervical cancer lesions. We hypothesize that EBV is not merely a commensal agent when present in malignant cervical lesions. DNA was extracted from cervical scrapings followed by nested PCR-based amplification. The patients were 66 women with high grade cervical intraepithelial neoplasia and 14 women with invasive cervical cancer. The control group consisted of 89 women with a normal Pap smear and colposcopy as well as a negative HPV DNA test. Analysis of our results, in conjunction with the work of other authors, leads us to propose that EBV is not merely a commensal agent when present in malignant cervical lesions. The presence of DNA from EBV is significantly associated with cervical cancer.
authors.affiliation: Colposcopy Section, Gynecology Department, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Nelly Beatriz Modós
authors.initials: NB
authors.lastname: Santos
authors.forename: Fabiola Elizabeth
authors.initials: FE
authors.lastname: Villanova
authors.forename: Priscila Maria
authors.initials: PM
authors.lastname: Andrade
authors.forename: Julisa
authors.initials: J
authors.lastname: Ribalta
authors.forename: José
authors.initials: J
authors.lastname: Focchi
authors.forename: Audrey Yumi
authors.initials: AY
authors.lastname: Otsuka
authors.forename: Ismael
authors.initials: I
authors.lastname: Dale Silva
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D065006:Papanicolaou Test; D016133:Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000821:virology*; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000821:virology*
chemical_list: D004279:DNA, Viral