Misexpression of miR-196a induces eye anomaly in Xenopus laevis.
miR-196a is located in the posterior trunk and plays a role in limb development. Here we show that miR-196a is able to induce eye anomaly in Xenopus laevis. Microinjection of synthetic miRNA precursor molecule for mammalian miR-196a into Xenopus embryo is sufficient for miR-196a overexpression during early development. The misexpression of miR-196a in anterior embryo led to dose-dependent eye anomalies, especially size reduction. In addition, the expression of ET, Rx1, Six3, Pax6, Lhx2, Optx2 and Ath5 in eye field or optic cup was also down-regulated. These results indicate that miR-196a can target gene(s) in the genetic network involved in eye formation, providing a potential tool for studying the mechanisms of eye development and diseases.
Qiu
Rong
R
The State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China. rongking2004@yahoo.com.cn
Liu
Ying
Y
Wu
Jane Y
JY
Liu
Kaili
K
Mo
Weichuan
W
He
Rongqiao
R
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D030541:Databases, Genetic; D005123:Eye / Q000196:embryology / Q000254:growth & development / Q000503:physiopathology; D005124:Eye Abnormalities / Q000503:physiopathology*; D005136:Eye Proteins / Q000378:metabolism; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000378:metabolism; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D010641:Phenotype; D012097:Repressor Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism; D014982:Xenopus laevis; D000097607:Homeobox Protein SIX3
D005136:Eye Proteins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D015534:Trans-Activators; D029867:Xenopus Proteins; D000097607:Homeobox Protein SIX3; C524416:Rax protein, Xenopus; C000622821:six6 protein, Xenopus; C121525:transcription repression factor ET, Xenopus