A novel herbal preparation desensitizes mesenteric afferents to bradykinin in the rat small intestine.
Herbal preparations are evolving as promising agents for the treatment of functional gastrointestinal disorders which are considered to be secondary to visceral hypersensitivity. We aimed to determine whether a new combination of six herbal extracts reduces the sensitivity of intestinal afferents in rat. Male Wistar rats (250-350 g, n = 6 per group) were gavaged with either vehicle or 2.5, 5 or 10 mL kg(-1) of STW 5-II, a herbal preparation which contains six extracts. Two hours later, animals were anaesthetized and extracellular multi-unit mesenteric afferent nerve recordings were obtained in the proximal jejunum in vivo. Afferent discharge to 5-hydroxy-tryptamine (5-HT) (5, 10, 20 and 40 microg kg(-1), i.v.), luminal distension (0-60 mmHg) and bradykinin (BK) (15, 30 and 60 microg kg(-1), i.v.) was recorded. At baseline, spontaneous afferent discharge was not different following pretreatment with the various doses of STW 5-II compared with vehicle. The pressure-dependent increase in afferent discharge to intraluminal ramp distension and the dose-dependent increase in afferent firing following 5-HT were also uninfluenced by STW 5-II pretreatment. In contrast, the afferent nerve responses to 15, 30 and 60 microg kg(-1) of BK were reduced following 10 mL kg(-1) STW 5-II with peaks at 106 +/- 19, 153 +/- 22 and 156 +/- 25 imp s(-1) compared with 160 +/- 15, 228 +/- 14 and 220 +/- 16 imp s(-1) following vehicle pretreatment (mean +/- SEM, P < 0.05). Intestinal afferent sensitivity to BK which plays a prime role in nociception was reduced following STW 5-II. Thus, STW 5-II may be of therapeutic use for conditions that involve neuronal hypersensitivity and the release of BK in the intestine.
Mueller
M H
MH
Institute of Surgical Research, Ludwig-Maximilan's University, Munich, Germany.
Gong
Q
Q
Kelber
O
O
Kasparek
M S
MS
Sibaev
A
A
Mansmann
U
U
Yuce
B
B
Li
Y Y
YY
Storr
M
M
Kreis
M E
ME
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D004594:Electrophysiology; D007421:Intestine, Small / Q000187:drug effects* / Q000294:innervation; D008297:Male; D008643:Mesentery / Q000294:innervation; D017288:Pain Threshold / Q000187:drug effects; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017833:Visceral Afferents / Q000187:drug effects*
D028321:Plant Preparations; D001920:Bradykinin