title: Pathological lesions and viral localization of influenza A (H5N1) virus in experimentally infected Chinese rhesus macaques: implications for pathogenesis and viral transmission.
abstract: Chinese rhesus macaques infected with influenza virus A/Tiger/Harbin/01/2002 (H5N1) developed acute interstitial pneumonia with diffuse alveolar damage. The results of virus isolation, reverse transcriptase polymerase chain reaction, immunohistochemistry, and in situ hybridization showed that the lung was the major target organ of the H5N1 virus infection. No virus was detected in the extrapulmonary organs. The results of immunohistochemistry and in situ hybridization also showed that pneumocytes and macrophages of the lower airway, not the ciliary epithelium of the trachea and bronchi, were the chief target cells in the lung tissue of the infected Chinese rhesus macaque. Our data indicate that the Chinese rhesus macaque is suitable as a new primate model for H5N1 virus research, especially for the study of H5N1 virus transmission. The predilection of the H5N1 virus to infect the lower airway suggests that the failure of the virus to attach to the ciliary epithelium of the trachea and bronchi may be a limiting factor in human-to-human transmissibility of the H5N1 virus.
authors.affiliation: Department of Pathology, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 5, Panjiayuan, Nanli, Chaoyang District, 100021 Beijing, People's Republic of China.
authors.forename: Yunxin
authors.initials: Y
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Chunshi
authors.initials: C
authors.lastname: Jia
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Dai
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Qi
authors.initials: Q
authors.lastname: Kong
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ma
authors.forename: Jiamei
authors.initials: J
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Xiao
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018562:Disease Transmission, Infectious; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000150:complications / Q000473:pathology* / Q000635:transmission* / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D017563:Lung Diseases, Interstitial / Q000473:pathology* / Q000821:virology*; D008253:Macaca mulatta; D008264:Macrophages / Q000821:virology; D020545:Respiratory Mucosa / Q000473:pathology / Q000821:virology