Metabolic and mitochondrial dysfunction in early mouse embryos following maternal dietary protein intervention.
Dietary supply of nutrients, both periconception and during pregnancy, influence the growth and development of the fetus and offspring and their health into adult life. Despite the importance of research efforts surrounding the developmental origins of health and disease hypothesis, the biological mechanisms involved remain elusive. Mitochondria are of major importance in the oocyte and early embryo, particularly as a source of ATP generation, and perturbations in their function have been related to reduced embryo quality. The present study examined embryo development following periconception exposure of females to a high-protein diet (HPD) or a low-protein diet (LPD) relative to a medium-protein diet (MPD; control), and we hypothesized that perturbed mitochondrial metabolism in the mouse embryo may be responsible for the impaired embryo and fetal development reported by others. Although the rate of development to the blastocyst stage did not differ between diets, both the HPD and LPD reduced the number of inner cell mass cells in the blastocyst-stage embryo. Furthermore, mitochondrial membrane potential was reduced and mitochondrial calcium levels increased in the 2-cell embryo. Embryos from HPD females had elevated levels of reactive oxygen species and ADP concentrations, indicative of metabolic stress and, potentially, the uncoupling of oxidative phosphorylation, whereas embryos from LPD females had reduced mitochondrial clustering around the nucleus, suggestive of an overall quietening of metabolism. Thus, although periconception dietary supply of different levels of protein is permissive of development, mitochondrial metabolism is altered in the early embryo, and the nature of the perturbation differs between HPD and LPD exposure.
Mitchell
Megan
M
Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, University of Adelaide, South Australia, Australia. megan.mitchell@adelaide.edu.au
Schulz
Samantha L
SL
Armstrong
David T
DT
Lane
Michelle
M
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects; D002970:Cleavage Stage, Ovum / Q000187:drug effects / Q000378:metabolism; D004032:Diet; D004044:Dietary Proteins / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000378:metabolism* / Q000503:physiopathology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D039382:Maternal Nutritional Physiological Phenomena / Q000187:drug effects / Q000502:physiology*; D008431:Maternal-Fetal Exchange / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D008954:Models, Biological; D011247:Pregnancy; D019289:Pyruvic Acid / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D004044:Dietary Proteins; D017382:Reactive Oxygen Species; D000244:Adenosine Diphosphate; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate