title: Identification and characterization of two alternative sigma factors of RNA polymerase in the deep-sea piezophilic bacterium Shewanella violacea, strain DSS12.
abstract: Two genes for alternative sigma factors, sigma(E2) and sigma(E3), classified in the extracytoplasmic function sigma family for RNA polymerases, were identified in the deep-sea piezophilic bacterium Shewanella violacea DSS12. Amino acid alignments revealed that the domains for transcriptional functions were comparatively conserved compared with Escherichia coli sigma(E) in both proteins. Core-binding analysis suggested that both proteins function as sigma factors.
authors.affiliation: Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC). k23322000@yahoo.co.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Abe
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics; D005798:Genes, Bacterial; D016415:Sequence Alignment; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics; D012808:Sigma Factor / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases