[Risk assessment for environmental release of genetically engineered microorganism m-CDS-1].
OBJECTIVE
To assess the environmental release risk of genetically engineered microorganism Sphingomonas sp. m-CDS-1.


METHODS
Pesticides detection, plates counting, most probable number-PCR (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) were used to study the pesticides degrading effects, the decline dynamics of m-CDS-1, and the effects of release of m-CDS-1 on community structure of soil microorganisms.


RESULTS
m-CDS-1 (1.01 x 10(7)CFU/g dry soil) could degrade both 10.71 mg/kg methyl parathion and 1.29 mg/kg carbofuran in 30 days. m-CDS-1 declined quickly with time, and could not be detected anymore in 30 days. Release of m-CDS-1 would not change the number of the cultivable microorganisms notably. The similarities of PCR-DGGE profiles between the pesticides applied treatments and the controls in 4, 11 and 30 days were 17.16 %, 49.81 % and 75.01 %, and the similarities between the m-CDS-1 released treatments and controls were 49.57 %, 38.3 % and 83.3 %, respectively. In 60 days, DGGE profiles of all treatments and controls had high similarities.


CONCLUSIONS
m-CDS-1 could degrade both pesticides remarkably in the field, and would not be the predominant population, and would not affect the community structure of soil microorganisms in the long term.
Jiang
Jiandong
J
College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. jiang_jjd@njau.edu.cn
Li
Rong
R
Guo
Xinqiang
X
Chen
Kai
K
Li
Shunpeng
S
D001673:Biodegradation, Environmental; D017753:Ecosystem; D005818:Genetic Engineering; D008743:Methyl Parathion / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000378:metabolism* / Q000502:physiology; D010575:Pesticides / Q000378:metabolism; D018570:Risk Assessment; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology / Q000592:standards; D012989:Soil Pollutants / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics / Q000378:metabolism*
D010575:Pesticides; D012987:Soil; D012989:Soil Pollutants; D008743:Methyl Parathion