(-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-kappaB.
People who regularly drink tea have been found to have a higher bone mineral density (BMD) and to be at less risk of hip fractures than those who do not drink it. Green tea catechins such as (-)-epigallocatechin gallate (EGCG) have been reported to increase osteogenic functioning in mesenchymal stem cells. However, its effect on osteoclastogenesis remains unclear. In this study, we investigated the effect of EGCG on RANKL-activation osteoclastogenesis and NF-kappaB in RAW 264.7, a murine preosteoclast cell line. EGCG (10-100 microM) significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in murine RAW 264.7 cells and bone marrow macrophages (BMMs). EGCG appeared to target osteoclastic differentiation at an early stage but had no cytotoxic effect on osteoclast precursors. In addition, it significantly inhibited RANKL-induced NF-kappaB transcriptional activity and nuclear translocation. We conclude that EGCG inhibits osteoclastogenesis through its activation of NF-kappaB.
Lin
Ru-Wei
RW
Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, 807 Kaohsiung, Taiwan, ROC.
Chen
Chung-Hwan
CH
Wang
Yan-Hsiung
YH
Ho
Mei-Ling
ML
Hung
Shao-Hung
SH
Chen
Ih-Sheng
IS
Wang
Gwo-Jaw
GJ
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053245:RANK Ligand / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D016328:NF-kappa B; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D002392:Catechin; C045651:epigallocatechin gallate