Protection against Yersinia pseudotuberculosis infection conferred by a Lactococcus lactis mucosal delivery vector secreting LcrV.
Herein, we sought to evaluate the potential of a recombinant Lactococcus lactis strain secreting the Yersinia pseudotuberculosis low-calcium response V (LcrV) antigen for mucosal vaccination against Yersinia infections. We showed that the recombinant strain induced specific systemic and mucosal antibody and cellular immune responses after intranasal immunization and protected mice against both oral and systemic Y. pseudotuberculosis infections. This constitutes the first proof of principle for a novel anti-Yersinia mucosal vaccination strategy using recombinant lactic acid bacteria.
Daniel
Catherine
C
Laboratoire des Bactéries Lactiques et Immunité des Muqueuses, Institut Fédératif de Recherche 142, Institut Pasteur de Lille, Lille F-59021, France. catherine.daniel@ibl.fr
Sebbane
Florent
F
Poiret
Sabine
S
Goudercourt
Denise
D
Dewulf
Joelle
J
Mullet
Chantal
C
Simonet
Michel
M
Pot
Bruno
B
D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D013294:Lactococcus lactis / Q000235:genetics*; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000737:chemistry; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics* / Q000276:immunology*; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000382:microbiology; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000276:immunology; D015012:Yersinia pseudotuberculosis Infections / Q000517:prevention & control*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; C043908:LcrV protein, Yersinia; D052899:Pore Forming Cytotoxic Proteins