VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.
VEGF-induced vascular barrier disruptions, formation of fenestrae and vesiculo-vacuolar organelles (VVOs), are involved in enhancing vascular permeability in metastatic and edematous diseases. Here, we analyzed vascular permeability enhanced by VEGFs with different receptor selectivity using biological and ultrastructural methods. VEGF-A(165), which stimulates both Flt-1 and KDR, induced the formation of both fenestrae and VVOs at a similar rate, while VEGF-F, a KDR-specific VEGF vammin, predominantly induced the formation of fenestrae with an approximately 5-fold more potent vascular permeability response than VEGF-A(165). This is the first report showing that VEGFs with different receptor selectivity evoke distinct changes in vascular ultrastructure.
Matsunaga
Yukiko
Y
Department of Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Yamazaki
Yasuo
Y
Suzuki
Hidenori
H
Morita
Takashi
T
D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000648:ultrastructure; D002199:Capillary Permeability / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000648:ultrastructure; D006168:Guinea Pigs; D046529:Microscopy, Electron, Transmission; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D014757:Viper Venoms / Q000494:pharmacology*
D042461:Vascular Endothelial Growth Factor A; D014757:Viper Venoms; C519402:vammin protein, Vipera ammodytes ammodytes