title: A possible role of vimentin on the cell surface for the activation of latent transforming growth factor-beta.
abstract: Latent TGF-beta (LTGF-beta) has to be converted to active TGF-beta for its activities. Previously, we reported that certain fragments of latency associated peptide (LAP) augmented LTGF-beta activation via increase in binding of LTGF-beta to the endothelial cell (EC) surface followed by cell-associated proteolysis. By searching for EC membrane proteins crosslinked with the LAP fragment, we identified the molecule bound to LAP fragment as vimentin. Moreover, the LAP fragment-induced LTGF-beta activation was attenuated by anti-vimentin antibody. These results indicate that binding of the LAP fragment to vimentin on the cell surface is indispensable for LTGF-beta activation by the LAP fragment.
authors.affiliation: Innovative Drug Research Laboratories, Research Division, Kyowa Hakko Kirin Co. Ltd., Tokyo 194-8533, Japan.
authors.forename: Yasutake
authors.initials: Y
authors.lastname: Nishida
authors.forename: Kenji
authors.initials: K
authors.lastname: Shibata
authors.forename: Motoo
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Sato
authors.forename: Mayumi
authors.initials: M
authors.lastname: Abe
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D053779:Latent TGF-beta Binding Proteins / Q000819:agonists* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
chemical_list: D053779:Latent TGF-beta Binding Proteins; D010455:Peptides; D016212:Transforming Growth Factor beta; D014746:Vimentin