In vivo labeling method using a genetic construct for nanoscale resolution microscopy.
We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.
Schröder
Jan
J
Leica Microsystems CMS, Mannheim, Germany. jan.schroeder@leica-microsystems.com
Benink
Hélène
H
Dyba
Marcus
M
Los
Georgyi V
GV
D045744:Cell Line, Tumor; D005456:Fluorescent Dyes; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D011554:Pseudopodia / Q000378:metabolism / Q000648:ultrastructure
D005456:Fluorescent Dyes; D019012:Integrin beta1