title: Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients.
abstract: Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients.
authors.affiliation: Department of Pharmacology, State University of Campinas, Campinas, SP, Brazil. ronaldo@uniararas.br
authors.forename: Ronaldo Luis
authors.initials: RL
authors.lastname: Thomasini
authors.forename: Juliana de Moraes
authors.initials: Jde M
authors.lastname: Martins
authors.forename: Daniela Corte
authors.initials: DC
authors.lastname: Parola
authors.forename: Sandra Helena Alves
authors.initials: SH
authors.lastname: Bonon
authors.forename: Ilka de Fátima Santana Ferreira
authors.initials: Ide F
authors.lastname: Boin
authors.forename: Luis Sérgio
authors.initials: LS
authors.lastname: Leonardi
authors.forename: Marília
authors.initials: M
authors.lastname: Leonardi
authors.forename: Sandra Cecília Botelho
authors.initials: SC
authors.lastname: Costa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D019349:Roseolovirus Infections / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D004279:DNA, Viral