title: Genetic variation in the long terminal repeat associated with the transition of Chinese equine infectious anemia virus from virulence to avirulence.
abstract: A highly virulent strain of equine infectious anemia virus (EIAV) lost its fatal virulence but retained the desired antigens during serial passage over 130 generations in leukocytes in vitro. We compared the long terminal repeat (LTR) sequences of the different generations and found that three stable genetic variations occurred in the transcriptional start site, the initial base of TAR, and the pre-mRNA cleavage site at the R-U5 boundary, respectively. These three mutations happened at the inflexion of virus pathogenicity loss; therefore, the function of these mutations needs to be further addressed.
authors.affiliation: Harbin Veterinary Research Institute of Chinese Academy of Agriculture Sciences (CAAS), 427 Maduan Street, Harbin, 150001, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wei
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Fan
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lu
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.forename: Wenhua
authors.initials: W
authors.lastname: Xiang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
authors.forename: Rongxian
authors.initials: R
authors.lastname: Shen
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018397:Equidae; D014644:Genetic Variation; D004860:Infectious Anemia Virus, Equine / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D008264:Macrophages / Q000821:virology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D020079:Terminal Repeat Sequences; D014774:Virulence
chemical_list: D012367:RNA, Viral