Cloning, expression, and protective immunity in mice of a gene encoding the diagnostic antigen P-29 of Echinococcus granulosus.
Taeniid tapeworm Echinococcus granulosus is the causative agent of Echinococcosis, an important zoonosis with worldwide distribution. In this study, a diagnostic antigen P-29 was cloned from E. granulosus and expressed in Escherichia coli. Sequence analysis showed that EgP-29 contains 717-bp open reading frame and encodes a protein of 238 amino acid residues with a predicted molecular weight of 27.1 kDa. The recombinant EgP-29 (rEgP-29) could be recognized with antimice sera in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgP-29 and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective immunity of 96.6% (P<0.05), compared with the control group. Thus, rEgP-29 protein is a promising candidate for an effective vaccine to prevent secondary echinococcosis.
Shi
Zhiyun
Z
Department of Medical Genetics and Cell Biology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China.
Wang
Yana
Y
Li
Zongji
Z
Li
Zhaoyu
Z
Bo
Yang
Y
Ma
Rui
R
Zhao
Wei
W
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers; D004443:Echinococcosis / Q000276:immunology / Q000517:prevention & control*; D048209:Echinococcus granulosus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000947:Antigens, Helminth; D017931:DNA Primers; C406723:P-29 antigen