An EGF-like peptide sequence from Dictyostelium enhances cell motility and chemotaxis.
Dictyostelium discoideum possesses more EGF-like (EGFL) domains than any other sequenced eukaryote. Here we show that a synthetic EGFL peptide (DdEGFL1) based upon an amino acid sequence from a cysteine-rich Dictyostelium protein, functions extracellularly to enhance random cell motility and cAMP-mediated chemotaxis in Dictyostelium by 625% and 85%, respectively, in strain NC4 and by 620% and 80% in strain AX3. Quinacrine inhibited peptide-enhanced random motility but not chemotaxis in strain AX3 providing evidence that PLA2 is the predominant regulator of this process. While LY294002 alone had no significant effect on either event, in combination with quinacrine it dramatically inhibited both processes suggesting that both PI3K and PLA2-mediated signaling are required for EGFL peptide-enhanced cell movement. DdEGFL1 also sustained the threonine phosphorylation of a 210kDa protein that is dephosphorylated during Dictyostelium starvation. Taken together, these results suggest an important role for certain EGFL peptides in Dictyostelium cell movement.
Huber
Robert
R
Department of Cell & Systems Biology, University of Toronto, Toronto, Ont., Canada M5S 3G5.
O'Day
Danton H
DH
D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement; D002633:Chemotaxis; D000242:Cyclic AMP / Q000378:metabolism; D004023:Dictyostelium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D011796:Quinacrine / Q000494:pharmacology
D010455:Peptides; D015800:Protozoan Proteins; D004815:Epidermal Growth Factor; D000242:Cyclic AMP; D011796:Quinacrine