Assessment of CE for the identification of microorganisms.
The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris-citrate-fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post-separation cultivation and sequential analysis was developed. Off-line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells.
Petr
Jan
J
Department of Analytical Chemistry, Faculty of Sciences, Palacký University in Olomouc, Olomouc, Czech Republic. petrjan1@gmail.com
Ryparová
Olga
O
Ranc
Václav
V
Hinnerová
Petra
P
Znaleziona
Joanna
J
Kowalska
Marta
M
Knob
Radim
R
Maier
Vítezslav
V
Frébort
Ivo
I
Lemr
Karel
K
Sevcík
Juraj
J
D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014325:Tromethamine / Q000737:chemistry
D004573:Electrolytes; D014325:Tromethamine; D011092:Polyethylene Glycols