In guinea pig sperm, aldolase A forms a complex with actin, WAS, and Arp2/3 that plays a role in actin polymerization.
Glycolytic enzymes have, in addition to their role in energy production, other functions in the regulation of cellular processes. Aldolase A has been reported to be present in sperm, playing a key role in glycolysis; however, despite its reported interactions with actin and WAS, little is known about a non-glycolytic role of aldolase A in sperm. Here, we show that in guinea pig spermatozoa, aldolase A is tightly associated to cytoskeletal structures where it interacts with actin, WAS, and Arp2/3. We show that aldolase A spermatozoa treatment increases their polymerized actin levels. In addition, we show that there is a direct correlation between the levels of polymerized actin and the levels of aldolase A-actin interaction. Our results suggest that aldolase A functions as a bridge between filaments of actin and the actin-polymerizing machinery.
Chiquete-Felix
Natalia
N
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), PC07360 México DF, Mexico.
Hernández
José Manuel
JM
Méndez
J Alfredo
JA
Zepeda-Bastida
Armando
A
Chagolla-López
Alicia
A
Mújica
Adela
A
D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D058105:Polymerization; D013094:Spermatozoa / Q000201:enzymology*; D051316:Wiskott-Aldrich Syndrome Protein / Q000378:metabolism*
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D051316:Wiskott-Aldrich Syndrome Protein; D005634:Fructose-Bisphosphate Aldolase