title: Study of solid-phase extraction for the determination of sequestering agents in river water by high-performance liquid chromatography.
abstract: Anion-exchange solid-phase extraction accompanied with high-performance liquid chromatography has been developed for the determination of six kinds of aminopolycarboxylic acids (APCAs) in river water [N-(2-hydroxyethyl)ethylenediaminetriacetate (HEDTA), ethylenediaminetetraacetate (EDTA), 1,3-propanediaminetetraacetate (PDTA), diethylenetriaminepentaacetate (DTPA), 1,2-propanediaminetetraacetate (MeEDTA), and O,O'-bis(2-aminoethyl)ethyleneglycoltetraacetate (GEDTA)]. The enrichment of APCAs using an anion-exchange cartridge was successfully done by the removal of anions, which competed with APCAs in anion-exchange processes. Barium chloride solution was added to river water and the mixture was passed through On Guard II Ag and H cartridges and then a Bond Elut Jr.SAX cartridge to enrich APCAs. After elution, APCAs were analyzed on two reversed phase C30 columns connected in series and detected with ultraviolet detection. The enrichment using solid-phase extraction permitted the determination of APCAs in river water at concentrations as low as 1nM. Good recoveries (83-111%) were obtained for each APCA by the standard addition method on three river water samples with high accuracy (RSD 1.8-9.5%). Applying this method, two kinds of APCAs, EDTA and DTPA, were determined in samples from the Oyabe and Senbo Rivers in Japan.
authors.affiliation: Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama 939-0363, Japan. tomoko.kenmei@pref.toyama.lg.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kemmei
authors.forename: Shuji
authors.initials: S
authors.lastname: Kodama
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Muramoto
authors.forename: Hironori
authors.initials: H
authors.lastname: Fujishima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D004533:Egtazic Acid / Q000032:analysis*; D005618:Fresh Water / Q000737:chemistry*; D006801:Humans; D008377:Maps as Topic; D004369:Pentetic Acid / Q000032:analysis*; D017670:Sodium Compounds / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D013464:Sulfuric Acids / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D017670:Sodium Compounds; D013464:Sulfuric Acids; D014874:Water Pollutants, Chemical; D004533:Egtazic Acid; D004369:Pentetic Acid; D004492:Edetic Acid; C033158:sulfuric acid