The detection of synergy between meropenem and polymyxin B against meropenem-resistant Acinetobacter baumannii using Etest and time-kill assay.
Time-kill assay and Etest testing for synergy of meropenem (MER) (1x MIC) plus polymyxin B (1/4, 1/2, and 1x MIC) were performed against 8 genetically unique MER-resistant clinical Acinetobacter baumannii isolates. Time-kill assay demonstrated synergy for all isolates, whereas Etest showed synergy in 5 isolates and indifference in 3.
Pankey
George A
GA
Infectious Disease Research, Ochsner Clinic Foundation, New Orleans, LA 70121, USA. gpankey@ochsner.org
Ashcraft
Deborah S
DS
D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019411:Clinical Laboratory Techniques; D015169:Colony Count, Microbial / Q000379:methods; D024881:Drug Resistance, Bacterial / Q000235:genetics* / Q000502:physiology; D004357:Drug Synergism; D006801:Humans; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D011112:Polymyxin B / Q000494:pharmacology*; D013845:Thienamycins / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D000077731:Meropenem; D011112:Polymyxin B