Formylglycine aldehyde Tag--protein engineering through a novel post-translational modification.
Oxidation of a specific cysteine residue to C(alpha)-formylglycine is a novel post-translational modification that is directed by a short recognition motif commonly found in pro- and eukaryotic sulfatases. As recently shown by C. Bertozzi and co-workers, this system can be employed in protein engineering to equip proteins with genetically encoded aldehyde tags for site-specific labeling, conjugation and immobilization.
Frese
Marc-André
MA
Faculty of Chemistry, Biochemistry I, Bielefeld University, Bielefeld, Germany.
Dierks
Thomas
T
D000447:Aldehydes / Q000737:chemistry*; D020816:Amino Acid Motifs; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011499:Protein Processing, Post-Translational
D000447:Aldehydes; D015335:Molecular Probes