[Relationship between expression of GYPC and TRIP3 genes and prognosis of acute lymphoblastic leukemia in children].
OBJECTIVE
To study the relationship of GYPC and TRIP3 gene expression and the prognosis of acute lymphoblastic leukemia (ALL) in children in order to explore the molecular biological mechanisms of recurrence and remission of ALL.


METHODS
Thirty-eight newly diagnosed ALL children were enrolled. Of the 38 patients, 31 achieved complete remission (CR) and 12 relapsed. Semi-quantitative RT-PCR was employed to measure blood GYPC and TRIP3 gene expression. Twenty blood samples from normal children were used as controls.


RESULTS
Blood GYPC expression in newly diagnosed ALL children was significantly higher than that in the control group (p<0.01) and the CR group (p<0.01). The expression of GYPC gene in the CR group was similar to that in the control group. Other than the control group (p<0.01) and the CR group (p<0.01), the GYPC expression of the relapse group was significantly higher than that in the newly diagnosed group (p<0.01). The CR group showed lower GYPC gene expression than the nonjremission group before treatment (p<0.05). Blood expression of TRIP3 gene in the newly diagnosed and the relapse groups was significantly lower than that in the control group (p<0.05). The CR group had increased TRIP3 gene expression compared with the control group (p<0.01) as well as the newly diagnosed and the relapse groups (p<0.01). Of the 38 newly diagnosed ALL children, the patients with positive TRIP3 expression showed higher remission rate than those with negative TRIP3 (p<0.05). The TRIP3 gene expression before treatment in patients who achieved CR was higher than that in non-remission patients (p<0.05).


CONCLUSIONS
A high GYPC gene expression is associated with an unfavorable outcome, in contrast, a high TRIP3 gene expression is associated with a favorable outcome in childhood ALL.
Zhang
Jian-Bai
JB
Department of Pediatrics, First Clinical Hospital Affiliated to Harbin Medical University, Harbin 150001, China.
Li
Xiao-Hui
XH
Ning
Fang
F
Guo
Xue-Song
XS
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006021:Glycophorins / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D056913:Mediator Complex Subunit 1; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000401:mortality; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
D006021:Glycophorins; C538855:MED1 protein, human; D056913:Mediator Complex Subunit 1; D014157:Transcription Factors