title: Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy.
abstract: The tongue consists of a complex, multiscale array of myofibers that comprise the anatomical underpinning of lingual mechanical function. 3-D myoarchitecture was imaged in mouse tongues with diffusion spectrum magnetic resonance imaging (DSI) at 9.4 T (b(max) 7000 smm, 150-microm isotropic voxels), a method that derives the preferential diffusion of water/voxel, and high-throughput (10 fps) two-photon microscope (TPM). Net fiber alignment was represented for each method in terms of the local maxima of an orientational distribution function (ODF) derived from the local diffusion (DSI) and 3-D structural autocorrelation (TPM), respectively. Mesoscale myofiber tracts were generated by alignment of the principal orientation vectors of the ODFs. These data revealed a consistent relationship between the properties of the respective ODFs and the virtual superimposition of the distributed mesoscale myofiber tracts. The identification of a mesoscale anatomical construct, which specifically links the microscopic and macroscopic spatial scales, provides a method for relating the orientation and distribution of cells and subcellular components with overall tissue morphology, thus contributing to the development of multiscale methods for mechanical analysis.
authors.affiliation: Massachusetts Institute of Technology, Department of Mechanical and Biological Engineering, Cambridge, Massachusetts 02139, USA.
authors.forename: Terry A
authors.initials: TA
authors.lastname: Gaige
authors.forename: Hyuk Sang
authors.initials: HS
authors.lastname: Kwon
authors.forename: Guangping
authors.initials: G
authors.lastname: Dai
authors.forename: Victor C
authors.initials: VC
authors.lastname: Cabral
authors.forename: Ruopeng
authors.initials: R
authors.lastname: Wang
authors.forename: Yoon Sung
authors.initials: YS
authors.lastname: Nam
authors.forename: Bevin P
authors.initials: BP
authors.lastname: Engelward
authors.forename: Van J
authors.initials: VJ
authors.lastname: Wedeen
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Gilbert
mesh_terms: D000465:Algorithms; D000818:Animals; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D066298:In Vitro Techniques; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique; D014059:Tongue / Q000166:cytology*