title: Rapid and simple profiling of lipoproteins by polyacrylamide-gel disc electrophoresis to determine the heterogeneity of low-density lipoproteins (LDLs) including small, dense LDL.
abstract: This study aimed to explore the potential of polyacrylamide-gel disc electrophoresis (PAGE) for lipoprotein profiling in clinical practice. Blood samples were collected from 146 patients with type 2 diabetes mellitus and lipid parameters were assayed by PAGE, including small, dense low-density lipoprotein (LDL) (n = 41), and triglyceride-rich lipoprotein remnant cholesterol (n = 37). We also used a commercial kit to measure small, dense LDL (n = 41). By PAGE, we obtained the percentage of the area under the curve (AUC %) of each peaks and calculated respective AUC% x total cholesterol (AUC%xTC) values. The calculated values of LDL-AUC%xTC, small LDL-AUC%xTC, and HDL-AUC%xTC values were correlated well with values from homogeneous assay for LDL-cholesterol, small, dense LDL-cholesterol, and HDL-cholesterol assays (r = 0.94, 0.81, and 0.89, respectively). PAGE combined with measurement of total cholesterol and triglycerides provides a rapid evaluation of anti- or pro-atherogenic lipoproteins and a simple profiling system for both the "quantity" and "quality" of lipoproteins, allowing a better assessment of the risk of coronary artery diseases. This article discusses several methods for simple and rapid lipid profiling and outlines some recent patents relevant to the methods.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Saitama Medical University, Saitama, Japan. nk.takanari@gmail.com
authors.forename: Takanari
authors.initials: T
authors.lastname: Nakano
authors.forename: Ikuo
authors.initials: I
authors.lastname: Inoue
authors.forename: Makoto
authors.initials: M
authors.lastname: Seo
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Takuya
authors.initials: T
authors.lastname: Awata
authors.forename: Tsugikazu
authors.initials: T
authors.lastname: Komoda
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Katayama
mesh_terms: D000180:Acrylic Resins; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004589:Electrophoresis, Disc / Q000379:methods*; D005260:Female; D006801:Humans; D008074:Lipoproteins; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood
chemical_list: D000180:Acrylic Resins; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D014280:Triglycerides; C016680:polyacrylamide gels; D002784:Cholesterol