title: The structure of rat liver vault at 3.5 angstrom resolution.
abstract: Vaults are among the largest cytoplasmic ribonucleoprotein particles and are found in numerous eukaryotic species. Roles in multidrug resistance and innate immunity have been suggested, but the cellular function remains unclear. We have determined the x-ray structure of rat liver vault at 3.5 angstrom resolution and show that the cage structure consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains. Each MVP monomer folds into 12 domains: nine structural repeat domains, a shoulder domain, a cap-helix domain, and a cap-ring domain. Interactions between the 42-turn-long cap-helix domains are key to stabilizing the particle. The shoulder domain is structurally similar to a core domain of stomatin, a lipid-raft component in erythrocytes and epithelial cells.
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamashita
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Sumizawa
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Kenji
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Masato
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D008099:Liver / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051381:Rats; D020394:Vault Ribonucleoprotein Particles / Q000737:chemistry*; D000099187:Major Vault Protein
chemical_list: D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein