title: [Roles of matrix metalloproteinases and tissue specific inhibitors of metalloproteinases in dentinogenic ghost cell tumor and ghost cell odontogenic carcinoma].
abstract: OBJECTIVE
  To investigate the roles of matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in dentinogenic ghost cell tumor (DGCT) and ghost cell odontogenic carcinoma (GCOC).
  
  
  METHODS
  The expressions of MMP-2, MMP-9, MMP-14, TIMP-1 and TIMP-2 were examined in 15 DGCT cases and 9 GCOC cases by immunohistochemistry. Their mRNA expression in one DGCT case and one GCOC case were investigated by RT-PCT.MMP-2 and MMP-9 protein activities in the two cases were analyzed by gelatin zymography.
  
  
  RESULTS
  MMP-9 and TIMP-1 expressions elevated greatly in GCOC, and there was a significant difference (P < 0.05) in TIMP-1 expression between GCOC and DGCT.Pro-MMP-9, MMP-9 activated form, pro-MMP-2, and MMP-2 activated forms were detected in the GCOC case, while pro-MMP-9 and MMP-9 activated form were very faint in the DGCT case. The mRNA level of MMP-9 elevated obviously in the GCOC case, which was similar to that of TIMP-1.
  
  
  CONCLUSIONS
  The elevated expression of MMP-9 and TIMP-1 may influence the behaviour of GCOC.
authors.affiliation: Department of Oral Pathology, West China College of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Geng
authors.forename: Yu
authors.initials: Y
authors.lastname: Ban
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Yang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Bao
authors.forename: Xin-Zhu
authors.initials: XZ
authors.lastname: Yi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003804:Dentin; D006801:Humans; D008339:Mandibular Neoplasms / Q000378:metabolism* / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D055815:Young Adult
chemical_list: D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases