[Immune responses induced by the suicidal DNA vaccines co-expressing the GP5 protein of PRRSV and the E2 protein of CSFV in mice].
Six recombinant plasmids co-expressing the wild-type GP5 gene or the codon-optimized GP5 gene (containing pan-DR epitope) of porcine reproductive and respiratory syndrome virus (PRRSV) and the E2 gene of classical swine fever virus (CSFV) or the E2 fused with the UL49 of pseudorabies virus (PrV) were constructed based on the suicidal DNA vaccine pSFV1CS-E2 described previously. Expression of GP5 and E2 was confirmed by indirect immunofluorescence assay. The immunogenicity of six plasmids was evaluated in BALB/c mouse model. For the six plasmids, low-level of E2 and GP5 protein specific antibodies could be detected in the sera of the immunized mice. Specific lymphoproliferative responses to the PRRSV or CSFV stimulation were induced in the splenocytes of the immunized mice as demonstrated by CFSE staining assay and WST-8 assay. Antigen specific IFN-gamma and L-4 secretion was detected in the splenocytes of some immunized mice by cytokine ELSIA. Fusion with the PrV UL49 in the suicidal vaccines induced significantly higher lymphoproliferative responses and cytokine secretion. Taken together, the suicidal DNA vaccines co-expressing GP5 and E2 could induce PRRSV and CSFV specific humoral and cell-mediated immune responses.
Sun
Jianfu
J
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy ofAgricultural Sciences, Harbin 150001, China.
Zhao
Heping
H
Li
Na
N
Sun
Yuan
Y
Xi
Zhaohe
Z
Zhou
Yanjun
Y
Wang
Yu
Y
Qi
Qiaofen
Q
Lu
Cheng
C
Qiu
Huaji
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D016207:Cytokines / Q000097:blood; D005260:Female; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D019444:Vaccines, DNA / Q000096:biosynthesis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000096:biosynthesis / Q000276:immunology*
D000914:Antibodies, Viral; D016207:Cytokines; C502892:VP22 protein, Pseudorabies virus; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV; C087086:glycoprotein E2, classical swine fever virus