In vivo magnetic resonance imaging of injected mesenchymal stem cells in rat myocardial infarction; simultaneous cell tracking and left ventricular function measurement.
To determine whether magnetic resonance imaging (MRI) can enable magnetically labeled mesenchymal stem cell (MSC) tracking and simultaneous in vivo functional data acquisition in rat models of myocardial infarction. Superparamagnetic iron oxide-laden human MSCs were injected into rat myocardium infarcted by cryoinjury 3 weeks after myocardial infarction. The control group received cell-free media injection. Before injection and for 3 months after, in vivo serial MRI was performed. Electrocardiography-gated gradient echo sequence MRI and cine MRI were performed for in vivo cell tracking and assessing cardiac function using left ventricular ejection fraction (LVEF), respectively. MRI revealed a persistent signal-void representing iron-laden MSCs until ten post-injection weeks. Serial follow-up MRI revealed that LVEF was significantly higher in the MSC injection group than in the control group. We conclude that MRI enables in vivo tracking of injected cells and evaluation of the long-term therapeutic potential of MSCs for myocardial infarction.
Kim
Young Jin
YJ
Department of Radiology and Research Institute of Radiological Science, Severance Hospital, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, 120-752 Seoul, South Korea.
Huh
Yong-Min
YM
Choe
Kyu Ok
KO
Choi
Byoung Wook
BW
Choi
Eun Jeong
EJ
Jang
Yangsoo
Y
Lee
Jae Myun
JM
Suh
Jin-Suck
JS
D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D003287:Contrast Media; D003911:Dextrans; D004195:Disease Models, Animal; D004562:Electrocardiography; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron; D019028:Magnetic Resonance Imaging, Cine; D058185:Magnetite Nanoparticles; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000473:pathology*; D010087:Oxides; D051381:Rats; D017207:Rats, Sprague-Dawley; D013318:Stroke Volume; D013997:Time Factors; D016277:Ventricular Function, Left
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide