Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.).
This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20 % which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.
N'Nan
Oulo
O
IRD, Montpellier Cedex, France.
Hocher
Valérie
V
Verdeil
Jean-Luc
JL
Konan
Jean-Louis
JL
Ballo
Koffi
K
Mondeil
Fanja
F
Malaurie
Bernard
B
D002470:Cell Survival / Q000502:physiology; D003059:Cocos / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005612:Freeze Drying / Q000379:methods*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology
D013395:Sucrose