[Influence of human trefoil factor 1 (hTFF1) fusion protein on gastric mucosal lesion in mice after burn injury].
OBJECTIVE
To observe the effect of hTFF1 fusion protein on gastric mucosal lesion in mice after burn injury.


METHODS
The pET32alpha-hTFF1 vector were transfected into E.coli Origami B (DE3), and rhTFF1 fusion protein was expressed by IPTG induction. Mice were inflicted with 30% TBSA full-thickness burn, then were divided into burn treatment (BT, with treatment of rhTEF1 fusion protein by gavage), burn control (BC, with treatment of isotonic saline by gavage), and normal control (NC) groups according to block randomized design. Gastric mucosal lesion, including appearance of gastric mucosa, injury index, pathological change, were compared between BT and BC groups before and after burn injury, and above indices in NC group were also examined.


RESULTS
The rhTFF1 fusion protein was expressed with good specificity confirmed with Western blot analysis. The gastric erosion rate was 77.8% and 22.2% respectively in BC and T group. The injury index was 6.2 +/- 2.0 and 2.0 +/- 1.2 respectively in BC and T group. Above indices in C group were 0.


CONCLUSIONS
The rhTFF1 fusion protein can be expressed in E.coli expression systems, which can obviously ameliorate gastric mucosal lesion in mice after burn injury.
Wu
Wei
W
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, the Third Military Medical University, Chongqing 400038, PR China.
Wang
Lin
L
Sun
Yong
Y
Lv
Shang-jun
SJ
Zhang
Yong
Y
Peng
Xi
X
D000818:Animals; D002056:Burns / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000473:pathology*; D006801:Humans; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins / Q000627:therapeutic use*; D014945:Wound Healing
D011993:Recombinant Fusion Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins