Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study.
A rapid and simple method of high performance liquid chromatography with UV detection for the quantification of bis(12)-hupyridone in rat blood has been developed and validated. Chromatographic separation was carried out in an Agilent Extend C(18) 5microm column (length, 250mm; inner diameter, 4.6mm) using a mixture of water-acetonitrile-trifluoroacetic acid (81:19:0.04, v/v/v) as the mobile phase at a flow rate of 1mL/min, with detection at 229nm. The method used for the bis(12)-hupyridone quantification showed linearity for concentration range of 0.1-7.5microg/mL with r(2)=0.9991. The limit of detection and quantification of this method were 0.05microg/mL and 0.1microg/mL, respectively. The intra- and inter-day variations of the analysis were less than 4.22% with standard errors less than 13.3%. The developed method was successfully applied to the pharmacokinetic study of bis(12)-hupyridone after intravenous administration of 5mg/kg and intraperitoneal administration of 10 and 20mg/kg in rats.
Yu
Hua
H
Department of Biochemistry, The Hong Kong University of Science & Technology, Kowloon, Hong Kong, People's Republic of China.
Li
Wen Ming
WM
Cheung
Man Chun
MC
Zuo
Zhong
Z
Carlier
Paul R
PR
Gu
Ze Ming
ZM
Chan
Kelvin
K
Huang
Min
M
Wang
Yi Tao
YT
Han
Yi Fan
YF
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D019540:Area Under Curve; D002800:Cholinesterase Inhibitors / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D004356:Drug Storage; D005615:Freezing; D006207:Half-Life; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects; D015394:Molecular Structure; D011786:Quality Control; D015363:Quinolones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D002800:Cholinesterase Inhibitors; D015363:Quinolones; C528260:bis(12)-hupyridone; D014867:Water; D014269:Trifluoroacetic Acid; C032159:acetonitrile