title: The effect of Propionibacterium acnes on maturation of dendritic cells derived from acne patients' peripherial blood mononuclear cells.
abstract: Propionibacterium acnes (P. acnes) has been implicated in the pathogenesis of acne vulgaris which is the most common cutaneous disorder. It has a proinflammatory activity and takes part in immune reactions modulating the Th1/Th2 cellular response. The exposure of dendritic cells (DCs) to whole bacteria, their components, cytokines or other inflammatory stimuli and infectious agents induces differentiation from immature DCs into antigen-presenting mature DCs. The aim of the study was to evaluate the capability of P. acnes to induce the maturation of DCs. We stimulated monocyte derived dendritic cells (Mo-DCs) from acne patients with various concetrations of heat-killed P. acnes (10(6)-10(8) bacteria/ml) cultured from acne lesions. The results showed an increase in CD80+/CD86+/DR+ and CD83+/CD1a+/DR+ cells percentage depending on the concetration of P. acnes. The expression of CD83 and CD80 (shown as the mean fluorescence intensity - MFI) increased with higher concetrations of P. acnes. There were also significant correlations between MFI of CD83, CD80, CD86 and concetration of P. acnes. The study showed that P. acnes in the concetration of 10(8) bacteria/ml is most effective in the induction of Mo-DCs maturation. Futher studies concerning the influence on the function of T cells are needed.
authors.affiliation: Department of Clinical Immunology, Medical University of Lublin, Poland. annamichalak@wp.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Michalak-Stoma
authors.forename: Jacek
authors.initials: J
authors.lastname: Tabarkiewicz
authors.forename: Alina
authors.initials: A
authors.lastname: Olender
authors.forename: Maria
authors.initials: M
authors.lastname: Juszkiewicz-Borowiec
authors.forename: Filip
authors.initials: F
authors.lastname: Stoma
authors.forename: Aldona
authors.initials: A
authors.lastname: Pietrzak
authors.forename: Piotr
authors.initials: P
authors.lastname: Pozarowski
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bartkowiak-Emeryk
mesh_terms: D000152:Acne Vulgaris / Q000097:blood* / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000382:microbiology* / Q000473:pathology*; D005453:Fluorescence; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D011425:Propionibacterium acnes / Q000276:immunology*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen