Tanacetum vulgare: antiherpes virus activity of crude extract and the purified compound parthenolide.
The present study demonstrated that the ethyl acetate extract and the isolated compound, parthenolide, from aerial parts of Tanacetum vulgare L. (Asteraceae), protected Vero cells from herpes simplex virus (HSV-1) infection in vitro. The extract and parthenolide were assayed against HSV-1 by the sulforhodamine B colorimetric assay and exhibited anti-HSV-1 activity with an EC(50) of 40 microg/mL and 0.3 microg/mL, respectively. In order to determine which stage of the virus-cell interaction was affected by parthenolide, the pure compound was used. No effect was observed when both viruses and cells were pretreated, or during early stages of infection, suggesting that parthenolide interfered with virus replication after the penetration stage, inhibiting approximately 40% of plaques formed at a concentration of 2.5 microg/mL when compared with an untreated control.
Onozato
Thelma
T
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá, PR Brazil.
Nakamura
Celso Vataru
CV
Cortez
Diógenes Aparício Garcia
DA
Dias Filho
Benedito Prado
BP
Ueda-Nakamura
Tânia
T
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D031203:Tanacetum / Q000737:chemistry; D014709:Vero Cells
D000998:Antiviral Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; C002669:parthenolide