title: Sulfated modification of the polysaccharides obtained from defatted rice bran and their antitumor activities.
abstract: Nine sulfated defatted rice bran polysaccharides (sRBPS), with various degrees of sulfation (DS) and carbohydrate content, were prepared by chlorosulfonic acid-pyridine (CSA-Pyr) method according to orthogonal test. Nine sulfated derivatives sRBPS were obtained and their antitumor activities were compared by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that when DS within the scope of 0.81-1.29, carbohydrate content in the range of 41.41-78.56%, sulfated derivatives exhibit relatively strong antitumor activity in vitro. The optimum modification conditions were reaction temperature of 70 degrees C, the ratio of chlorosulfonic acid to pyridine of 1:4 and the reaction time of 2h.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Li
authors.forename: Zhengxing
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005227:Fatty Acids / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D012275:Oryza / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D005227:Fatty Acids; D011134:Polysaccharides; D013431:Sulfates