[Evaluation of the humoral immune response to Strongyloides venezuelensis antigens].
Strongyloidiasis affects 30 million people in 70 countries. This enteral parasitosis is usually diagnosed using parasitological tests based on hydrotropism or thermotropism of larvae eliminated in feces, but these tests have been shown to have low sensitivity. In this study, antigenic extracts were tested by means of ELISA, immunoblotting and IFI, using filariform larvae of Strongyloides venezuelensis, a parasite of rodents that shows cross-reactions with Strongyloides stercoralis epitopes. Sensitivity of 89, 85 and 57% for the ELISA reaction and 100, 100 and 96% for immunoblotting with the SAL, ZWIP and ZW antigens, and specificity of 90, 60 and 81% for ELISA and 96, 92 and 91% for immunoblotting with the same antigens, were found in these assays.
Rigo
Carla Rodrigues
CR
Bolsista de Mestrado da Fundação de Amparo à Pesquisa do Estado de São Paulo, São Paulo, SP, Brazil.
Lescano
Susana Zevallos
SZ
Marchi
Cláudia Regina de
CR
Amato Neto
Vicente
V
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D007814:Larva / Q000276:immunology; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis / Q000276:immunology*
D000947:Antigens, Helminth