[Establishment of spinal muscular atrophy cell model by RNAi].
OBJECTIVE
To establish spinal muscular atrophy (SMA) cell model by blocking the expression of SMN1 gene with shRNA.


METHODS
The recombinant SMN1 shRNA expression vector was constructed. SMA cell model was established by human mesenchymal stem cells(hMSCs) that the vector was transfected into were differentiated to neuron like cells (NLCs).At the same time the control groups were established that the shRNA-0 vector was transfected into and no vector was transfected into. The expression of fl-SMN and delta7-SMN mRNA was observed by RT-PCR analysis. The expression of fl-SMN protein was detected by Western blot.


RESULTS
The cells of all the groups were neuron like cells after being differentiated and the protein expression of NSE and NF was positive. The expression of fl-SMN and delta7-SMN mRNA and protein of NLCs in each group was upregulated (P<0.05), but the expression of delta7-SMN mRNA and protein in SMA model group was lower than that in the control group (P<0.05). The expression of delta7-SMN mRNA between the groups had no statistical difference (P>0.05).


CONCLUSIONS
The NLCs, which recombinant SMN1 shRNA expression vector was transfected into, can be regarded as SMA cell model.
Yang
Xiao-Su
XS
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China. sjnk_xy@yahoo.com.cn
Hu
Yi-Min
YM
Xiao
Bo
B
D002454:Cell Differentiation; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008954:Models, Biological; D009474:Neurons / Q000166:cytology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014897:Spinal Muscular Atrophies of Childhood / Q000235:genetics / Q000473:pathology; D055533:Survival of Motor Neuron 1 Protein / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; C526471:SMN1 protein, human; D055533:Survival of Motor Neuron 1 Protein