title: Nontypeable Haemophilus influenzae adhesin protein E: characterization and biological activity.
abstract: The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on pulmonary challenge than did mice immunized with a control peptide. Finally, anti-PE mouse antibodies from vaccinated mice prevented NTHi adhesion. Our data suggest that the ubiquitous adhesin PE plays an important role in the pathogenesis of NTHi infection.
authors.affiliation: Medical Microbiology, Department of Laboratory Medicine, Malmö University Hospital, Lund University, Malmö, Sweden.
authors.forename: Elena
authors.initials: E
authors.lastname: Ronander
authors.forename: Marta
authors.initials: M
authors.lastname: Brant
authors.forename: Emily
authors.initials: E
authors.lastname: Eriksson
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.forename: Oskar
authors.initials: O
authors.lastname: Hallgren
authors.forename: Gunilla
authors.initials: G
authors.lastname: Westergren-Thorsson
authors.forename: Arne
authors.initials: A
authors.lastname: Forsgren
authors.forename: Kristian
authors.initials: K
authors.lastname: Riesbeck
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D003627:Data Interpretation, Statistical; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics; D006192:Haemophilus Infections / Q000276:immunology / Q000382:microbiology; D006193:Haemophilus influenzae / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D007378:Interleukins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1