title: Quantification of phenyllactic acid in wheat sourdough using high resolution gas chromatography-mass spectrometry.
abstract: In this study, high-resolution gas chromatography-mass spectrometry (HRGC-MS) was successfully used to quantify the level of phenyllactic acid produced by Lactobacillus plantarum strains during sourdough fermentation. Investigation of samples collected during fermentation revealed that the production of phenyllactic acid occurs throughout the growth of L. plantarum in sourdough, but the highest production rate was observed during the logarithmic growth phase. The highest amount, that is, 33.47 mg of phenyllactic acid/kg of dough, was measured in sourdough fermented by the antifungal strain L. plantarum FST 1.7. Sourdoughs fermented by different L. plantarum strains contained different amounts of phenyllactic acid, thus indicating that the production is strain-dependent. Phenylacetic acid was also detected during sourdough analysis, thus showing that the HRGC-MS protocol developed is suitable for the detection not only of phenyllactic acid, but also of a broader range of phenolic acids that are highly relevant, but present in very low amounts in sourdough.
authors.affiliation: Department of Food Science, Food Technology and Nutrition, and National Food Biotechnology Centre, Cork, Ireland.
authors.forename: Liam Anthony Matthew
authors.initials: LA
authors.lastname: Ryan
authors.forename: Fabio
authors.initials: F
authors.lastname: Dal Bello
authors.forename: Michael
authors.initials: M
authors.lastname: Czerny
authors.forename: Peter
authors.initials: P
authors.lastname: Koehler
authors.forename: Elke Karin
authors.initials: EK
authors.lastname: Arendt
mesh_terms: D001939:Bread / Q000032:analysis / Q000382:microbiology*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D007773:Lactates / Q000032:analysis* / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000378:metabolism; D014908:Triticum / Q000382:microbiology*
chemical_list: D007773:Lactates; C017648:3-phenyllactic acid