Immunosuppressive property of dried human amniotic membrane.
OBJECTIVE
To report the immunosuppressive property of dry human amniotic membrane (dHAM).


METHODS
Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation.


RESULTS
dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1).


CONCLUSIONS
The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.
Park
Choul Yong
CY
Department of Ophthalmology, Dongguk University, Koyang, South Korea.
Kohanim
Sahar
S
Zhu
Lei
L
Gehlbach
Peter L
PL
Chuck
Roy S
RS
D000650:Amnion / Q000276:immunology*; D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D003890:Desiccation; D006801:Humans; D007108:Immune Tolerance; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D017252:CD3 Complex; C485468:Cd3e protein, mouse