title: Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.).
abstract: This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20 % which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.
authors.affiliation: IRD, Montpellier Cedex, France.
authors.forename: Oulo
authors.initials: O
authors.lastname: N'Nan
authors.forename: Valérie
authors.initials: V
authors.lastname: Hocher
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Verdeil
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Konan
authors.forename: Koffi
authors.initials: K
authors.lastname: Ballo
authors.forename: Fanja
authors.initials: F
authors.lastname: Mondeil
authors.forename: Bernard
authors.initials: B
authors.lastname: Malaurie
mesh_terms: D002470:Cell Survival / Q000502:physiology; D003059:Cocos / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005612:Freeze Drying / Q000379:methods*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology
chemical_list: D013395:Sucrose