title: Seropositivity to Burkholderia pseudomallei does not reflect the development of cell-mediated immunity.
abstract: Cell-mediated immunity to Burkholderia pseudomallei, the causative agent of melioidosis, provides protection from disease progression. An indirect haemagglutination assay was used to detect antibodies to B. pseudomallei in 1500 healthy donors in an endemic region of Australia. Lymphocyte proliferation, activation and cytokine expression to B. pseudomallei antigen were determined in eight donors who were seropositive and in eight age- and sex-matched controls. In North Queensland, 2.5% of the population was seropositive for B. pseudomallei, which is less than half that which was previously described. Of clinical significance was the observation that while 75% of the seropositive individuals had increased lymphocyte proliferation to B. pseudomallei antigens, there were no significant differences observed in lymphocyte activation or production of cytokines.
authors.affiliation: School of Veterinary and Biomedical Sciences, James Cook University, Queensland 4811, Australia.
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Lazzaroni
authors.forename: Jodie L
authors.initials: JL
authors.lastname: Barnes
authors.forename: Natasha L
authors.initials: NL
authors.lastname: Williams
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Govan
authors.forename: Robert E
authors.initials: RE
authors.lastname: Norton
authors.forename: Justin T
authors.initials: JT
authors.lastname: LaBrooy
authors.forename: Natkunam
authors.initials: N
authors.lastname: Ketheesan
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008554:Melioidosis / Q000276:immunology*; D008875:Middle Aged
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D016207:Cytokines