Spatio-temporal control of neural epithelial cell migration and epithelium-to-mesenchyme transition during avian neural tube development.
As opposed to the neural crest, the neural epithelium is generally viewed as a static and cohesive structure. Here, using an ex vivo system free of the environmental influences and physical constraints encountered in the embryo, we show that neural epithelial cells are on the contrary intrinsically motile, although they do not undergo spontaneous epithelium-to-mesenchyme transition and display molecular and cellular characteristics distinct from those of neural crest cells. However, they can be instructed to undergo epithelium-to-mesenchyme conversion independently of the acquisition of neural crest traits. Migration potentialities of neural epithelial cells are transient and are progressively restricted during neural tube development. Restriction of cell migration is irreversible and can be in part accounted for by increase in N-cadherin in cellular junctions and in cell polarity. In conclusion, our study reveals that the neural epithelium is a highly flexible tissue in which cells are maintained cohesive under the control of a combination of extrinsic factors and physical constraints.
Duband
Jean-Loup
JL
University Pierre et Marie Curie, Laboratory of Developmental Biology, 75005 Paris, France. jean-loup.duband@upmc.fr
Blavet
Cédrine
C
Jarov
Artem
A
Fournier-Thibault
Claire
C
D000818:Animals; D018959:CD57 Antigens / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D008648:Mesoderm / Q000196:embryology* / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology / Q000196:embryology*; D011784:Quail
D018959:CD57 Antigens; D015820:Cadherins; D005353:Fibronectins