Influence of baking enzymes on antimicrobial activity of five bacteriocin-like inhibitory substances produced by lactic acid bacteria isolated from Lithuanian sourdoughs.
OBJECTIVE
To evaluate the effect of four different baking enzymes on the inhibitory activity of five bacteriocin-like inhibitory substances (BLIS) produced by lactic acid bacteria (LAB) isolated from Lithuanian sourdoughs.


RESULTS
The overlay assay and the Bioscreen methods revealed that the five BLIS exhibited an inhibitory effect against spore germination and vegetative outgrowth of Bacillus subtilis, the predominant species causing ropiness in bread. The possibility that the observed antibacterial activity of BLIS might be lost after treatment with enzymes used for baking purposes was also examined.


CONCLUSIONS
The enzymes tested; hemicellulase, lipase, amyloglucosidase and amylase had little or no effect on the majority of the antimicrobial activities associated with the five BLIS studied.


CONCLUSIONS
This study suggests a potential application in the sourdough baking industry for these antimicrobial producing LAB strains in the control of B. subtilis spore germination and vegetative outgrowth.
Narbutaite
V
V
Commensals and Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich, UK.
Fernandez
A
A
Horn
N
N
Juodeikiene
G
G
Narbad
A
A
D000681:Amylases / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology; D001430:Bacteriocins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D001939:Bread / Q000382:microbiology*; D005656:Fungal Proteins / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007778:Lactobacillus / Q000737:chemistry* / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D010376:Pediococcus / Q000737:chemistry* / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology
D001430:Bacteriocins; D005656:Fungal Proteins; D008049:Lipase; D000681:Amylases; D006026:Glycoside Hydrolases; C023305:hemicellulase; D005087:Glucan 1,4-alpha-Glucosidase