Effect of sirolimus on urinary bladder cancer T24 cell line.
BACKGROUND
Sirolimus is recently reported to have antitumour effects on a large variety of cancers. The present study was performed to investigate sirolimus's ability to inhibit growth in T24 bladder cancer cells.


METHODS
T24 bladder cancer cells were treated with various concentrations of sirolimus. MTT assay was used to evaluate the proliferation inhibitory effect on T24 cell line. The viability of T24 cell line was determined by Trypan blue exclusion analysis.


RESULTS
Sirolimus inhibits the growth of bladder carcinoma cells and decreases their viability. Significant correlations were found between cell proliferation and sirolimus concentration (r = 0.830; p < 0.01) as well as between cell viability and sirolimus concentration (r = -0.896; p < 0.01).


CONCLUSIONS
Sirolimus has an anti-proliferation effect on the T24 bladder carcinoma cell line. The information from our results is useful for a better understanding sirolimus's anti-proliferative activity in the T24 bladder cancer cell line.
Pinto-Leite
Rosario
R
Genetic Service, Cytogenetic laboratory, Hospital center of Trás-os-Montes and Alto Douro, Vila Real, Portugal. mlleite@chtmad.min-saude.pt
Botelho
Pedro
P
Ribeiro
Eufemia
E
Oliveira
Paula A
PA
Santos
Lucios
L
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D020123:Sirolimus / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D000903:Antibiotics, Antineoplastic; D020123:Sirolimus