title: Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer.
abstract: BACKGROUND
  We develop a multiplex quantitative real-time PCR for synchronized analysis of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) to investigate relative mtDNA abundance in paired normal and cancerous breast tissues.
  
  
  METHODS
  The amounts of nDNA and mtDNA in 102 tissue samples were quantified for both glyceraldehype-3-phosphodehydrogenase (GAPDH) gene and mtDNA encoded ATPase (MTATP) 8 gene. The average threshold cycle (Ct) number values of the nDNA and mtDNA were used to calculate relative mtDNA content in breast tissues.
  
  
  RESULTS
  The median delta Ct (DeltaCt) and the median mtDNA content for normal and cancerous breast tissues were 6.73 and 2.54, as well as 106.50 and 5.80 (P = 0.000, respectively). The mtDNA content was decreased in 82% of cancerous breast tissues compared with the normal ones. The changes were associated with hormone receptor status.
  
  
  CONCLUSIONS
  Our finding suggests that decreased mtDNA content in breast cancer may have diagnostic and prognostic value for the disease.
authors.affiliation: Laboratory for Prenatal Medicine and Gynecologic Oncology, Department of Biomedicine, Women's Hospital, University of Basel, Hebelstrasse 20, Room Nr. 416, 4031 Basel, Switzerland.
authors.forename: Alex Xiu-Cheng
authors.initials: AX
authors.lastname: Fan
authors.forename: Ramin
authors.initials: R
authors.lastname: Radpour
authors.forename: Mahdi Montazer
authors.initials: MM
authors.lastname: Haghighi
authors.forename: Corina
authors.initials: C
authors.lastname: Kohler
authors.forename: Peng
authors.initials: P
authors.lastname: Xia
authors.forename: Sinuhe
authors.initials: S
authors.lastname: Hahn
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Holzgreve
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Zhong
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology*; D004272:DNA, Mitochondrial / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases