[alpha1,2-fucosyltransferase gene transfection influences on biological behavior of ovarian carcinoma-derived RMG-I cells].
To explore the effect of alpha1,2-fucosyltransferase (alpha1,2-FT) gene on the biological behavior of ovarian carcinoma cells, the expression vector pcDNA3.1 -HFUT-H which containing human alpha1,2-FT gene coding region was constructed and transfected into ovarian carcinoma cell line RMG-I. alpha1,2-FT gene expression, Lewis y antigen expression, alpha1,2-FT activity, rates of cell growth and tumorogenesis in nude mice were compared before and after RMG-I being transfected. The transfection of alpha1,2-FT gene significantly increased the expression of alpha1,2-FT gene and Lewis y antigen in RMG-I cells. After the transfection, RMG-I cells showed remarkable improvement of alpha1,2-FT activity, cell proliferation, the ability of colony formation in soft agar and tumorogenesis in nude mice. The growth rates of alpha1,2-FT gene transfected RMG-I cells were obviously increased, presenting that the proportion of the cells in G2-M and S phases was obviously increased from 2.05% to 7.32% and from 23.95% to 33.27% respectively, and that of the cells in G1 phase was obviously decreased from 74.14% to 59.46%, as compared with the control group. These findings indicate that alpha1,2-FT and Lewis y antigen have the ability to enhance the proliferation and elevate the survival rates of RMG-I cells, which can promote the genesis and development of ovarian carcinoma.
Hao
Ying Ying
YY
Department of Gynecology and Obstetrics, The Shengjing Hospital of China Medical University, Shenyang 110004, China.
Lin
Bei
B
Zhao
Yue
Y
Zhang
Yu Hua
YH
Li
Fei Fei
FF
Diao
Bin
B
Ou
Yang Ling
YL
Zhang
Shu Lan
SL
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D005647:Fucosyltransferases