title: Culture-independent analysis of microbial succession during composting of Swine slurry and mushroom cultural wastes.
abstract: Bacterial diversity and the composition of individual communities during the composting process of swine and mushroom cultural wastes in a field-scale composter (Hazaka system) were examined using a PCR-based approach. The composting process was divided into six stages based on recorded temperature changes. Phylogenetic analysis of eighty 16S rRNA sequences from uncultured composting bacterial groups revealed the presence of representatives from three divisions, including plant pathogenic bacteria, high-molecule-degrading bacteria and spore-forming bacteria. The plant pathogen A. tumefaciens gradually decreased in abundance during the composting process and eventually disappeared during the thermophilic and cooling stage. A bacterium homologous to Bacillus humi first appeared at the early thermophilic stage and was established at the intermediate thermophilic, post-thermophilic, and cooling stages. It was not possible to isolate the B. humi during any of the stages using general culture techniques.
authors.affiliation: Research Institute of Agriculture and Life Science,Gyeongsang National University, Chinju 660-701, Korea.
authors.forename: Kye Man
authors.initials: KM
authors.lastname: Cho
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: Renukaradhya K
authors.initials: RK
authors.lastname: Math
authors.forename: Shah Md Asraful
authors.initials: SM
authors.lastname: Islam
authors.forename: Devaiah M
authors.initials: DM
authors.lastname: Kambiranda
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Myoung Geun
authors.initials: MG
authors.lastname: Yun
authors.forename: Ji Joong
authors.initials: JJ
authors.lastname: Cho
authors.forename: Jong Ok
authors.initials: JO
authors.lastname: Kim
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Han Dae
authors.initials: HD
authors.lastname: Yun
mesh_terms: D000363:Agaricales; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D044822:Biodiversity; D015169:Colony Count, Microbial; D008372:Manure / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013552:Swine; D013696:Temperature
chemical_list: D008372:Manure; D012336:RNA, Ribosomal, 16S