[Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata].
OBJECTIVE
To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.


METHODS
The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.


CONCLUSIONS
The results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).
Xue
Jian-Ping
JP
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China. xuejp2000@yahoo.com.cn
Shi
Jing-Ying
JY
D029044:Achyranthes / Q000166:cytology* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*
D011134:Polysaccharides