A novel pro-apoptosis gene PNAS4 that induces apoptosis in A549 human lung adenocarcinoma cells and inhibits tumor growth in mice.
The gene PNAS4 is a high conservative gene that shares high homology of sequence in various organisms from plants to animals. We found overexpression of human PNAS4 induced apoptosis and arrested cell cycle in S phase in A549 human lung adenocarcinoma cells. In C57BL/6 mice model of Lewis lung carcinoma, overexpression of mouse PNAS4 significantly suppressed tumor growth and prolonged survival time through induction of tumor cell apoptosis, exhibiting effective antitumor. Our original investigations in vitro and vivo indicated PNAS4 is a novel pro-apoptosis gene, which could be used as a potential target of cancer biotherapy in future.
Yan
Fei
F
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, Sichuan, PR China.
Gou
Lantu
L
Yang
Jinliang
J
Chen
Lijuan
L
Tong
Aiping
A
Tang
Minghai
M
Yuan
Zhu
Z
Yao
Shaohua
S
Zhang
Peng
P
Wei
Yuquan
Y
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D019759:Carbon-Nitrogen Lyases; D018827:Carcinoma, Lewis Lung / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; C541601:PNAS4 protein, mouse; D019759:Carbon-Nitrogen Lyases; C000604991:DESI2 protein, human