title: Anti-inflammatory and anti-nociceptive effects of Zeyheria montana (Bignoniaceae) ethanol extract.
abstract: In this work, the analgesic and anti-inflammatory activities of Zeyheria montana Mart. ethanol leaf extract were investigated at doses of 75, 150 and 300 mg/kg body weight. In the analgesic assay, against a chemical stimulus in mice, acetic acid-induced writhes were significantly inhibited by the extract at doses of 75 mg/kg (67.27%), 150 mg/kg (49.38%) and 300 mg/kg (82.87%). Also, a vigorous decrease in hyperalgesia was observed when measured after 2 h and 6 h of lipopolysaccharide stimulation of rats for all doses of extract tested. Z. montana extract, at doses of 75 and 300 mg/kg, caused very slight central analgesia in rats submitted to thermal stimulus, particularly noticeable at 30 min following treatment. The anti-inflammatory activity of Z. montana extract on carrageenan-induced oedema in rats was evaluated. The oedema development, measured at 180 min following carrageenan intraplantar injection, was significantly reduced by all tested doses: 75 mg/kg (33.30%), 150 mg/kg (45.80%) and 300 mg/kg (75.00%). The LD50 value was greater than 2000 mg/kg. These results demonstrated that the ethanol extract from Z. montana leaf possesses anti-nociceptive and anti-inflammatory activities, which could be of relevance for the pharmacological control of pain and inflammatory processes.
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Guenka
authors.forename: R C
authors.initials: RC
authors.lastname: Gomes
authors.forename: V L
authors.initials: VL
authors.lastname: Melo
authors.forename: C R R
authors.initials: CR
authors.lastname: Kitanishi
authors.forename: P S
authors.initials: PS
authors.lastname: Pereira
authors.forename: S C
authors.initials: SC
authors.lastname: França
authors.forename: L B
authors.initials: LB
authors.lastname: Couto
authors.forename: R O
authors.initials: RO
authors.lastname: Beleboni
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000302:isolation & purification / Q000494:pharmacology; D006930:Hyperalgesia / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D000431:Ethanol