[Effect of operational modes on community structure of type I methanotroph in the cover soil of municipal solid waste landfill].
Type I methanotroph is crucial for methane oxidization and it responses fast to the changes in environment. In this study, 16S rDNA-based denaturing gradient gel electrophoresis (DGGE) gene fingerprint technology was applied to investigate the effect of operational modes, i. e. high-density polyethylene liner (HDPE) isolation or subsurface irrigation of landfill leachate and vegetation, on community structure and diversity of type I methanotroph in soils covering municipal solid waste landfill. 16S rDNA based phylogenetic analysis reveals type I methanotroph in all tested soils belongs to Methylobacter. According to Shannon-Wiener diversity index and principal component analysis, landfill leachate subsurface irrigation and vegetation have more impact on type I methanotroph community structure and diversity than HDPE liner isolation does, and they reduce type I methanotroph diversity. Leachate irrigation is supposed to inhibit the growth of Methylobacter population. Community structure of type I methanotroph in landfill cover soil isolated by HDPE, i.e. invaded by landfill gas, shifts during long-term gas interference. When cover age is 1.5 years old, Shannon-Wiener diversity index of type I methanotroph reaches its maximum.
Yu
Ting
T
Key Laboratory of Yangtze River Water Environment, Ministry of Education, State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. yttracy1983@yahoo.com.cn
He
Pin-Jing
PJ
Lü
Fan
F
Shao
Li-Ming
LM
D002947:Cities; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D008697:Methane / Q000378:metabolism*; D020580:Methylobacterium / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011157:Population Dynamics; D012037:Refuse Disposal / Q000379:methods*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012987:Soil; D008697:Methane