Hypoglycemic activity of extracts and compounds from the leaves of Hintonia standleyana and H. latiflora: potential alternatives to the use of the stem bark of these species.
The CH(2)Cl(2)-MeOH (1:1) extract of the leaves of Hintonia standleyana and H. latiflora caused significant decrease in blood glucose levels in both normal and streptozotozin (STZ)-induced diabetic rats when compared with vehicle-treated groups (p < 0.05). These extracts were not toxic to mice according to the Lorke criteria. From the hypoglycemic extract of H. standleyana, two new 4-phenylcoumarins, namely, 6''-O-acetyl-5-O-beta-d-galactopyranosyl-7,4'-dihydroxy-4-phenylcoumarin (1) and 6''-O-acetyl-5-O-beta-d-galactopyranosyl-7,3',4'-trihydroxy-4-phenylcoumarin (2), were obtained. The analogous extract of H. latiflora yielded the new 5-O-[beta-d-xylopyranosyl-(1-->6)-beta-d-glucopyranosyl]-7,4'-dimethoxy-4-phenylcoumarin (3) along with several known compounds, including ursolic acid and desoxycordifolinic acid. Phenylcoumarins 1 and 2 showed hypoglycemic activity. HPLC profiles of the leaf extracts of both plants revealed the presence of known hypoglycemic phenylcoumarins as well as chlorogenic acid. The overall results have indicated that the leaves of H. standleyana and H. latiflora possess similar antidiabetic potential to their stem bark. Therefore, the leaves from these species could represent an alternative to the use of their stem bark, which, in turn, would contribute to the conservation of these Mexican medicinal plants.
Cristians
Sol
S
Departamento de Farmacia, Facultad de Quimica, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City, D.F. 04510, Mexico, and Jardin Botanico, Instituto de Biologia Universidad Nacional Autonoma de Mexico, Mexico City, D.F. 04510, Mexico.
Guerrero-Analco
José A
JA
Pérez-Vásquez
Araceli
A
Palacios-Espinosa
Francisco
F
Ciangherotti
Carlos
C
Bye
Robert
R
Mata
Rachel
R
D000818:Animals; D003374:Coumarins / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008800:Mexico; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*; D013311:Streptozocin / Q000494:pharmacology
D003374:Coumarins; D007004:Hypoglycemic Agents; C094411:4-phenylcoumarin; D013311:Streptozocin