title: Functional fatty fish supplemented with grape procyanidins. Antioxidant and proapoptotic properties on colon cell lines.
abstract: This work shows the properties of grape procyanidins with additional anticarcinogenic properties for increasing the shelf life of functional seafood preparations. Galloylated procyanidins (100 ppm, 2.7 mean degree of polymerization, 25% galloylation) extended the shelf life of minced horse mackerel muscle stored at 4 degrees C more than 8 days compared to controls without addition of polyphenols. The levels of endogenous alpha-tocopherol, EPA, and DHA of fish muscle were also preserved after 10 days at 4 degrees C. Therefore, the presence of procyanidins increased the stability of a product based on minced fish muscle during cold storage and maintained its functionality associated with the presence of polyunsaturated fatty acids and alpha-tocopherol. In addition, grape procyanidins showed a significant capacity to induce apoptosis in colon cancer cells (HT29 cell line) while being inactive in noncancer control cells (IEC-6). Thus, the product based on fatty fish muscle supplemented with grape procyanidins appears to be a stable functional food offering the combined action of omega-3 fatty acids and natural polyphenols.
authors.affiliation: Instituto de Investigaciones Marinas del CSIC, Eduardo Cabello 6, E-36208 Vigo, Spain. medina@iim.csic.es
authors.forename: I
authors.initials: I
authors.lastname: Medina
authors.forename: S
authors.initials: S
authors.lastname: Lois
authors.forename: D
authors.initials: D
authors.lastname: Lizárraga
authors.forename: M
authors.initials: M
authors.lastname: Pazos
authors.forename: S
authors.initials: S
authors.lastname: Touriño
authors.forename: M
authors.initials: M
authors.lastname: Cascante
authors.forename: J L
authors.initials: JL
authors.lastname: Torres
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D003106:Colon / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004041:Dietary Fats / Q000592:standards*; D019587:Dietary Supplements; D005399:Fishes; D005519:Food Preservation / Q000379:methods; D006801:Humans; D008517:Phytotherapy; D044945:Proanthocyanidins / Q000494:pharmacology / Q000627:therapeutic use*; D017747:Seafood; D027843:Vitis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D004041:Dietary Fats; D044945:Proanthocyanidins