title: In situ genetic analysis of cellular chimerism.
abstract: Copy number variants are a recently discovered source of large-scale genomic diversity present in all individuals. We capitalize on these inherent genomic differences, focusing on deletion polymorphisms, to develop informative fluorescence in situ hybridization probes with the ability to unequivocally distinguish between donor and recipient cells in situ. These probes are accurate, specific, highly polymorphic and, notably, can be used to assign genetic identity in situ in a completely gender-independent fashion. We anticipate that these polymorphic deletion probes will be useful in further understanding the dynamics of cellular chimerism after transplantation, including the details of chronic organ rejection, post-transplant lymphoproliferative disorder and graft-versus-host disease, and in optimizing future tissue engineering and pluripotent stem cell therapies.
authors.affiliation: Department of Pathology, Massachusetts General Hospital, 55 Fruit Street, Boston, Massachusetts 02114, USA.
authors.forename: David
authors.initials: D
authors.lastname: Wu
authors.forename: Quynh
authors.initials: Q
authors.lastname: Vu
authors.forename: Anhthu
authors.initials: A
authors.lastname: Nguyen
authors.forename: James R
authors.initials: JR
authors.lastname: Stone
authors.forename: Hannah
authors.initials: H
authors.lastname: Stubbs
authors.forename: Georgiana
authors.initials: G
authors.lastname: Kuhlmann
authors.forename: Lynette M
authors.initials: LM
authors.lastname: Sholl
authors.forename: A John
authors.initials: AJ
authors.lastname: Iafrate
mesh_terms: D016026:Bone Marrow Transplantation; D002678:Chimera / Q000235:genetics*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D015335:Molecular Probes; D011110:Polymorphism, Genetic
chemical_list: D015335:Molecular Probes