Cooperative DNA probing using a beta-cyclodextrin-DNA conjugate and a nucleobase-specific fluorescent ligand.
A single nucleotide polymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying beta-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby beta-CyD, only when the ligand recognizes the specific base displayed in the gap.
Ihara
Toshihiro
T
Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. toshi@chem.kumamoto-u.ac.jp
Uemura
Asuka
A
Futamura
Akika
A
Shimizu
Masamichi
M
Baba
Noriyuki
N
Nishizawa
Seiichi
S
Teramae
Norio
N
Jyo
Akinori
A
D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D013053:Spectrophotometry; D013379:Substrate Specificity; D047392:beta-Cyclodextrins / Q000737:chemistry*
D015342:DNA Probes; D005456:Fluorescent Dyes; D008024:Ligands; D047392:beta-Cyclodextrins; D004247:DNA