Protein-resistant polyurethane via surface-initiated atom transfer radical polymerization of oligo(ethylene glycol) methacrylate.
Protein-resistant polyurethane (PU) surfaces were prepared by surface-initiated simultaneous normal and reverse atom transfer radical polymerization (s-ATRP) of poly(oligo(ethylene glycol) methacrylate) (poly (OEGMA)). Oxygen plasma treatment was employed for initial activation of the PU surface. The grafted polymer chain length was adjusted by varying the molar ratio of monomer to sacrificial initiator in solution from 5:1 to 200:1. The modified PU surfaces were characterized by water contact angle, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Protein adsorption experiments from tris-buffered saline (TBS) and plasma were carried out to evaluate the protein-resistance of the surfaces. Adsorption from single and binary protein solutions as well as from plasma was significantly reduced after modification. Adsorption decreased with increasing poly(OEGMA) chain length. Fibrinogen (Fg) adsorption on the 200:1 monomer/initiator surface was in the range of 3-33 ng/cm(2) representing 96-99% reduction compared with the unmodified PU. Fg adsorption from 0.01-10% plasma was as low as 1-5 ng/cm(2). Moreover, binary protein adsorption experiments using Fg and lysozyme (Lys) showed that protein size is a factor in the protein resistance of these surfaces.
Jin
Zhilin
Z
Department of Chemical Engineering and School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada.
Feng
Wei
W
Zhu
Shiping
S
Sheardown
Heather
H
Brash
John L
JL
D000327:Adsorption; D032701:Biomimetics / Q000379:methods*; D002021:Buffers; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids; D011140:Polyurethanes / Q000737:chemistry*; D012996:Solutions; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D002021:Buffers; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D012996:Solutions; C528061:poly(poly(ethylene glycol)methacrylate); D014867:Water; D011092:Polyethylene Glycols; D005340:Fibrinogen