Immunological properties of a fusion protein containing nucleocapsid protein and glycoprotein Gn of Hantaan virus.
Escherichia coli and the baculovirus Bac-to-Bac system were used to express chimeric gene GnS0.7 consisting of glycoprotein Gn gene and the 0.7 kb fragment of S genome segment carrying nucleoprotein (N) gene of Hantaan virus (HTNV). The expressed fusion protein GnN0.7 was recognized by monoclonal antibodies (MAbs) to HTNV Gn glycoprotein and N protein, respectively. Its molecular mass as determined by Western blot analysis corresponded to the predicted value. The mice immunized with insect Spodoptera frugiperda (Sf9) cells transfected with recombinant baculovirus carrying GnS0.7 gene, produced serum antibodies with titer up to 3,200 as assayed by immunofluorescence. Moreover, immunized mice showed positive proliferation index for splenocytes stimulated with HTNV Gn and N, respectively. These results indicated that insect Sf9 cells infected with the recombinant baculovirus expressed a fully biologically active fusion protein that elicited not only humoral but also cellular immune response in mice. Hence, this protein may be used as a genetically engineered subunit HTNV vaccine representing efficacious and safe alternative to traditional vaccines.
Luo
W
W
Department of Microbiology, Fourth Military Medical University, 17 West Changle Rd, Xi'an 710032, PR China.
Zhang
F
F
Yan
Y
Y
Wu
X
X
Liu
Y
Y
Bai
W
W
Zhao
Q
Q
Wang
H
H
Xu
Z
Z
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology* / Q000821:virology; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; C111782:nucleocapsid protein, Hantaan virus