Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients.
Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients.
Thomasini
Ronaldo Luis
RL
Department of Pharmacology, State University of Campinas, Campinas, SP, Brazil. ronaldo@uniararas.br
Martins
Juliana de Moraes
Jde M
Parola
Daniela Corte
DC
Bonon
Sandra Helena Alves
SH
Boin
Ilka de Fátima Santana Ferreira
Ide F
Leonardi
Luis Sérgio
LS
Leonardi
Marília
M
Costa
Sandra Cecília Botelho
SC
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D019349:Roseolovirus Infections / Q000175:diagnosis*; D055815:Young Adult
D004279:DNA, Viral