Cryptococcus tepidarius sp. nov., a thermotolerant yeast species isolated from a stream from a hot-spring area in Japan.
Anamorphic basidiomycetous yeast strains M9962T and M9963 were isolated from water samples collected from a small stream in Ohwakudani, Hakone, a hot-spring area in Japan. These belonged to a single species and were phylogenetically closely related to Bullera lagerstroemiae, although the ability to form ballistoconidia was not observed. Based on sequence analyses of the D1/D2 domain of the LSU rDNA and ITS regions and differences in G+C content, the name Cryptococcus tepidarius sp. nov. Takashima, Sugita, Toriumi et Nakase (Trichosporonales, Tremellomycetes, Basiomycota) is proposed for these isolates, with strain M9962T (=SP-5T =JCM 11965T =CBS 9427T) as the type strain. The strains grew in YM broth at 47 degrees C and in YM broth with the pH adjusted to 1.5 by HCl, indicating that the species is thermotolerant and acid-tolerant.
Takashima
Masako
M
Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Sugita
Takashi
T
Toriumi
Yoshimi
Y
Nakase
Takashi
T
D001482:Base Composition; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007564:Japan; D010802:Phylogeny; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer