title: Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae.
abstract: Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.
authors.affiliation: Research Information Center for Extremophile, Rikkyo University, 3-34-1 Nishiikebukuro, Toshima, Tokyo 171-8501, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Osami
authors.initials: O
authors.lastname: Misumi
authors.forename: Kousuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Yamato
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Keiji
authors.initials: K
authors.lastname: Nishida
authors.forename: Fumi
authors.initials: F
authors.lastname: Yagisawa
authors.forename: Sousuke
authors.initials: S
authors.lastname: Imamura
authors.forename: Masaki
authors.initials: M
authors.lastname: Yoshida
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Mori
authors.forename: Kan
authors.initials: K
authors.lastname: Tanaka
authors.forename: Haruko
authors.initials: H
authors.lastname: Kuroiwa
authors.forename: Tsuneyoshi
authors.initials: T
authors.lastname: Kuroiwa
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism*; D015870:Gene Expression; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D018087:Plastids / Q000378:metabolism; D000461:Rhodophyta / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
chemical_list: D020418:Algal Proteins