title: Mucosal immunity in mice induced by orally administered transgenic rice.
abstract: Transgenic plants are efficient means of producing and delivering oral vaccines. Rice material shown previously to express the Chlamydophila psittaci (Cp. psittaci) antigen (MOMP) fused to the B subunit of Escherichia coli heat-labile enterotoxin (LTB) was fed to mice and the resulting immune response was investigated. Oral immunization of mice with the transgenic rice elicited MOMP-specific sera IgG and IgA antibodies, a strong increase of the lymphoproliferative response, and significant levels of IFN-gamma, TGF-beta and IL-2 production. Furthermore, the immunization of mice with transgenic rice elicited strong cytotoxic T lymphocyte (CTL) responses in vitro. These results demonstrated that plant-made LTB-MOMP fusion protein could induce significant humoral and cellular Th1 and Th3 immune responses. Moreover, transgenic rice immunization induced partial protection (53.3%) against a lethal challenge with the highly virulent Cp. psittaci 6BC strain in a BALB/c mouse model. These results suggest that expression of protective antigens of Cp. psittaci in transgenic rice has potential as an edible vaccine against avain chlamydiosis.
authors.affiliation: Laboratory of Molecular Biology, College of Plant Science, Agricultural Division, Jilin University, 1068 Qinglong Road, Changchun 130062, PR China. zxx_yzg@163.com
authors.forename: Xiuxiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Ziguo
authors.initials: Z
authors.lastname: Yuan
authors.forename: Qing
authors.initials: Q
authors.lastname: Duan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics*; D002691:Chlamydophila psittaci / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D018928:Immunity, Mucosal; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D009956:Psittacosis / Q000276:immunology / Q000517:prevention & control; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D022462:Vaccines, Edible / Q000008:administration & dosage* / Q000235:genetics*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D022462:Vaccines, Edible; C078167:OMPA outer membrane proteins