Alternative strategies for the derivation of human embryonic stem cell lines and the role of dead embryos.
The therapeutic potential for human embryonic stem cells (hESC) drives intense public and scientific interest. However, the classical approach for derivation of hESC entails the destruction of human embryos. Controversial ethical issues and correspondingly restrictive federal policies in many countries have prompted the development of alternative approaches for the isolation of hESC. Here, several different strategies are discussed with a focus on the harvesting of live hESC from dead embryos.
Gavrilov
Svetlana
S
Division of Experimental Therapeutics, Collage of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Papaioannou
Virginia E
VE
Landry
Donald W
DW
D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D033041:Embryo Research / Q000941:ethics* / Q000331:legislation & jurisprudence; D006801:Humans; D051379:Mice; D053652:Nuclear Transfer Techniques; D033581:Stem Cell Transplantation / Q000941:ethics* / Q000331:legislation & jurisprudence / Q000639:trends; D020858:Tissue and Organ Harvesting / Q000941:ethics / Q000379:methods* / Q000639:trends; D014481:United States