title: Mosaic analysis of stem cell function and wound healing in the mouse corneal epithelium.
abstract: BACKGROUND
  The mouse corneal epithelium is a continuously renewing 5-6 cell thick protective layer covering the corneal surface, which regenerates rapidly when injured. It is maintained by peripherally located limbal stem cells (LSCs) that produce transient amplifying cells (TACs) which proliferate, migrate centripetally, differentiate and are eventually shed from the epithelial surface. LSC activity is required both for normal tissue maintenance and wound healing. Mosaic analysis can provide insights into LSC function, cell movement and cell mixing during tissue maintenance and repair. The present study investigates cell streaming during corneal maintenance and repair and changes in LSC function with age.
  
  
  RESULTS
  The initial pattern of corneal epithelial patches in XLacZ+/- X-inactivation mosaics was replaced after birth by radial stripes, indicating activation of LSCs. Stripe patterns (clockwise, anticlockwise or midline) were independent between paired eyes. Wound healing in organ culture was analysed by mosaic analysis of XLacZ+/- eyes or time-lapse imaging of GFP mosaics. Both central and peripheral wounds healed clonally, with cells moving in from all around the wound circumference without significant cell mixing, to reconstitute striping patterns. Mosaic analysis revealed that wounds can heal asymmetrically. Healing of peripheral wounds produced stripe patterns that mimicked some aberrant striping patterns observed in unwounded corneas. Quantitative analysis provided no evidence for an uneven distribution of LSC clones but showed that corrected corneal epithelial stripe numbers declined with age (implying declining LSC function) but stabilised after 39 weeks.
  
  
  CONCLUSIONS
  Striping patterns, produced by centripetal movement, are defined independently and stochastically in individual eyes. Little cell mixing occurs during the initial phase of wound healing and the direction of cell movement is determined by the position of the wound and not by population pressure from the limbus. LSC function declines with age and this may reflect reduced LSCs numbers, more quiescent LSCs or a reduced ability of older stem cells to maintain tissue homeostasis. The later plateau of LSC function might indicate the minimum LSC function that is sufficient for corneal epithelial maintenance. Quantitative and temporal mosaic analyses provide new possibilities for studying stem cell function, tissue maintenance and repair.
authors.affiliation: Division of Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh, UK. richardm@hgu.mrc.ac.uk
authors.forename: Richard L
authors.initials: RL
authors.lastname: Mort
authors.forename: Thaya
authors.initials: T
authors.lastname: Ramaesh
authors.forename: Dirk A
authors.initials: DA
authors.lastname: Kleinjan
authors.forename: Steven D
authors.initials: SD
authors.lastname: Morley
authors.forename: John D
authors.initials: JD
authors.lastname: West
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D019573:Epithelium, Corneal / Q000166:cytology* / Q000502:physiology; D005260:Female; D016850:Limbus Corneae / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D014945:Wound Healing / Q000502:physiology*; D049951:X Chromosome Inactivation