title: Detection of Equid herpesvirus 9 DNA in the trigeminal ganglia of a Burchell's zebra from the Serengeti ecosystem.
abstract: Equid herpesvirus 9 (EHV-9) was isolated from a herd of Thomson's gazelles affected by encephalitis. The natural host of EHV-9 is unknown, but zebras are suspected to be the source of infection in gazelles. To prove this hypothesis, we analyzed 43 sera from Burchell's zebras (Equus burchelli) and 21 Thomson's gazelles (Gazella thomsoni) from the Serengeti ecosystem for neutralizing antibodies. Seven zebra sera were positive for EHV-1, EHV-9 and EHV-1 from Grevy's zebra strains T965 and T616. The trigeminal ganglia of 17 other Burchell's zebras and one Thomson's gazelle were tested by EHV-9 gB and EHV-1 ICP0-specific nested polymerase chain reaction (PCR). PCR sequencing confirmed that one zebra ganglion was positive for EHV-9. These results suggest that the Burchell's zebras were exposed to EHV-9 and latently infected.
authors.affiliation: Institute of Virology, FU Berlin, Germany. borchers@zedat.fu-berlin.de
authors.forename: Kerstin
authors.initials: K
authors.lastname: Borchers
authors.forename: Dietmar
authors.initials: D
authors.lastname: Lieckfeldt
authors.forename: Arne
authors.initials: A
authors.lastname: Ludwig
authors.forename: Hideto
authors.initials: H
authors.lastname: Fukushi
authors.forename: George
authors.initials: G
authors.lastname: Allen
authors.forename: Robert
authors.initials: R
authors.lastname: Fyumagwa
authors.forename: Richard
authors.initials: R
authors.lastname: Hoare
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000866:Antelopes; D004279:DNA, Viral / Q000145:classification*; D017753:Ecosystem; D018397:Equidae; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D013636:Tanzania / Q000453:epidemiology; D014276:Trigeminal Nerve / Q000821:virology*; D018140:Varicellovirus / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins