Absolute CD4+ cell count using a plastic microchip and a microscopic cell counter.
We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD4(+) T-cells with the use of plastic microchips. This new system is an adaptation of a "no-lyse, no-wash," volumetric single platform assay, and absolute CD4(+) counts are determined with the use of a microscopic scanning cell counter. To assess the CD4(+) count test precision and linearity of the system, measured CD4(+) counts were compared with two other reference assays (single and dual platform flow cytometry) with the use of 123 clinical samples including samples obtained from 35 HIV-infected patients, and artificially diluted samples. A correlation between the results from the use of the new method and from the use of the two other reference assays was r = 0.98 for the clinical samples. A dilution test of the new method demonstrated a linearity of r >or= 0.99, with coefficients of variation <or=7.6% for all concentration levels. Our findings suggest that the new CD4(+) counting device can be potentially be applied for other diagnostic procedures that measure quantities of characteristic antigens or other materials on cells.
Bae
Sook Young
SY
Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, Korea University, Seoul, Korea.
Park
Hae Chul
HC
Oh
Jun Seo
JS
Yoon
Soo-Young
SY
Park
Dae Won
DW
Choi
In Keun
IK
Kim
Hae Joong
HJ
Oh
Jong Hyun
JH
Hur
Dae Sung
DS
Chung
Chanil
C
Chang
Jun Keun
JK
Robinson
J Paul
JP
Lim
Chae Seung
CS
D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D015658:HIV Infections / Q000097:blood / Q000276:immunology; D006801:Humans; D046208:Microchip Analytical Procedures; D015203:Reproducibility of Results