Synergistic neurotoxicity induced by methylmercury and quercetin in mice.
Methylmercury (MeHg) is a highly neurotoxic pollutant, whose mechanisms of toxicity are related to its pro-oxidative properties. A previous report showed under in vivo conditions the neuroprotective effects of plants of the genus Polygala against MeHg-induced neurotoxicity. Moreover, the flavonoid quercetin, isolated from Polygala sabulosa, displayed beneficial effects against MeHg-induced oxidative damage under in vitro conditions. In this study, we sought for potential beneficial effects of quercetin against the neurotoxicity induced by MeHg in Swiss female mice. Animals were divided into six experimental groups: control, quercetin low dose (5 mg/kg), quercetin high dose (50 mg/kg), MeHg (40 mg/L, in tap water), MeHg+quercetin low dose, and MeHg+quercetin high dose. After the treatment (21 days), a significant motor deficit was observed in MeHg+quercetin groups. Biochemical parameters related to oxidative stress showed that the simultaneous treatment with quercetin and MeHg caused a higher cerebellar oxidative damage when compared to the individual exposures. MeHg plus quercetin elicited a higher cerebellar lipid peroxidation than MeHg or quercetin alone. The present results indicate that under in vivo conditions quercetin and MeHg cause additive pro-oxidative effects toward the mice cerebellum and that such phenomenon is associated with the observed motor deficit.
Martins
Roberta de P
Rde P
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Trindade, 88040-900 Florianópolis, SC, Brazil.
Braga
Hugo de C
Hde C
da Silva
Aline P
AP
Dalmarco
Juliana B
JB
de Bem
Andreza F
AF
dos Santos
Adair Roberto S
AR
Dafre
Alcir L
AL
Pizzolatti
Moacir G
MG
Latini
Alexandra
A
Aschner
Michael
M
Farina
Marcelo
M
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D008767:Methylmercury Compounds / Q000633:toxicity*; D051379:Mice; D009420:Nervous System / Q000187:drug effects*; D018384:Oxidative Stress; D011794:Quercetin / Q000633:toxicity*
D008767:Methylmercury Compounds; D011794:Quercetin