title: Validation and comparison of an extrapolysaccharide (EPS)-based in-house ELISA and the PanBio melioidosis rapid cassette test-kits for serodiagnosis of melioidosis in a non-endemic area.
abstract: Detection of anti-Burkholderia pseudomallei antibodies in sera from melioidosis patients still represents a keystone in the confirmation of the clinical diagnosis, especially in non-endemic areas. An in-house assay was compared to lateral flow assays for the rapid detection of melioidosis-specific IgG or IgM. Employing 50 positive sera from patients and 200 negative sera from blood donors, sensitivity of the ELISA, the IgG and IgM assay were 84.0%, 90.0% and 84.0%, respectively. Specificity ranged from 98.0% (ELISA) to 99.5% (IgM assay). The application of the described diagnostic assays is a suitable method for the serodiagnosis of melioidosis in a non-endemic area.
authors.affiliation: Bundeswehr Institute of Microbiology, Neuherbergstr. 11, 80937 Munich, Germany. wolfsplettstoesser@bundeswehr.org
authors.forename: Wolf D
authors.initials: WD
authors.lastname: Splettstoesser
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Frangoulidis
authors.forename: Savithiri D
authors.initials: SD
authors.lastname: Puthucheary
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008554:Melioidosis / Q000175:diagnosis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D012698:Serologic Tests; D014195:Travel
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic