Effect of stress-proteins on survival of bone marrow mesenchymal stem cells after intramyocardial transplantation against the background of postinfarction heart remodeling.
We studied the presence of colony-forming cells in cell culture from rat heart 40 days after experimental myocardial infraction. The mean cellularity in this pathology was 12+/-8 cell/cm2, which is 20-fold lower than in intact myocardium. Transplantation of mesenchymal stem cells into the remodeling myocardium restored the pool of colony-forming cells. This effect depended on the state of transplanted cells. After transplantation of mesenchymal stem cells with low content of stress proteins, 6+/-2 colonies were detected, while after transplantation of cells with high content of hsp70 and hsp60 stress proteins (modified mesenchymal stem cells) 18+/-5 colonies were found, the mean cellularity of the corresponding cultured being 946+/-267 and 1926+/-123 cell/cm2. The positive effect of modified mesenchymal stem cells was observed on days 4 and 7 after transplantation. We conclude that postinfarction remodeling mobilized the total pool of regional stem cells; mesenchymal stem cells with high content of hsp70 and hsp60 demonstrated highest survival rate after intramyocardial transplantation.
Afanasiev
S A
SA
Institute of Cardiology, Tomsk Research Center, Siberian Division of Russian Academy of Medical Sciences, Russia.
Falaleeva
L P
LP
Rebrova
T U
TU
Suslova
T E
TE
Popov
S V
SV
Karpov
R S
RS
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D009203:Myocardial Infarction / Q000473:pathology*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling / Q000502:physiology*
D006360:Heat-Shock Proteins