[Cloning and functional analysis of two regulators involved in regulation of cellulase formation in Trichoderma koningii].
ACEI and Xyr1 are two regulators that potentially involve in regulation of cellulases and xylanases formation in Trichoderma reesei, they compete for a binding site in the xyn1 (Xylanase1-encoding) gene promoter. To further investigate the mechanism for the transcriptional regulation of cellulases, DNA-binding domains of both ACEI and Xyr1 in T. Koningii were expressed from E. coli. They both showed bindings to the cbh1 promoter fragment (-304 bp to -18 bp) by electrophoresis mobility shift assays, suggesting ACEI and Xyr1 not only compete for binding to xyn1 promoter but also to cbh1 promoter.
Ling
Min
M
Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Qin
Yongling
Y
Li
Nan
N
Liang
Zhiqun
Z
D001665:Binding Sites; D002480:Cellulase / Q000096:biosynthesis*; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
D015534:Trans-Activators; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases