title: Delineating melanoma using multimodal polarized light imaging.
abstract: BACKGROUND
  Melanoma accounts for 3% of all skin cancers but causes 83% of skin cancer deaths. The first step in treatment of melanoma is the removal of the lesions, usually by surgical excision. Currently most lesions are removed without intraoperative margin control. Post-operative methods inspect 1-2% of the surgical margin and are prone to sampling errors. In this study we evaluate the use of reflectance and fluorescence polarization imaging for the demarcation of melanoma in thick fresh skin excisions.
  
  
  METHODS
  Pigmented lesions clinically suspicious for melanoma were elliptically excised with proper margins. Elliptical surgical excisions were vertically bisected along the short axis of the specimen into two halves in the middle of the pigmented lesions. The vertically bisected tumor face was imaged. After that, one half of the sample was briefly stained in aqueous 2 mg/ml solution of tetracycline, whereas another half was stained in 0.2 mg/ml aqueous solution of methylene blue. Then both specimens were reimaged. Reflectance images were acquired in the spectral range between 390 and 750 nm. Fluorescence images of the tetracycline-stained tissue were excited at 390 nm and registered between 450 and 700 nm. Fluorescence of the methylene blue-stained samples was excited at 630 nm and registered between 650 and 750 nm. After imaging, the tissue was processed for standard H&E histopathology. The resulting histological and optical images were compared to each other.
  
  
  CONCLUSIONS
  Our findings demonstrate that both tetracycline and methylene blue are suitable for imaging dysplastic and benign nevi. Melanoma is better delineated in the samples stained in methylene blue. Accurate and rapid delineation of melanoma in standard fresh surgical excisions appears feasible.
authors.affiliation: Boston Veterans Affairs Medical Center (Jamaica Plains Campus), Boston, Massachusetts, USA.
authors.forename: Zeina
authors.initials: Z
authors.lastname: Tannous
authors.forename: Munir
authors.initials: M
authors.lastname: Al-Arashi
authors.forename: Sonali
authors.initials: S
authors.lastname: Shah
authors.forename: Anna N
authors.initials: AN
authors.lastname: Yaroslavsky
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002863:Chromogenic Compounds; D004416:Dysplastic Nevus Syndrome / Q000473:pathology* / Q000601:surgery; D005454:Fluorescence Polarization; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008297:Male; D008545:Melanoma / Q000473:pathology* / Q000601:surgery; D008751:Methylene Blue; D008859:Microscopy, Polarization / Q000379:methods*; D008875:Middle Aged; D010865:Pilot Projects; D012878:Skin Neoplasms / Q000473:pathology* / Q000601:surgery; D013752:Tetracycline
chemical_list: D002863:Chromogenic Compounds; D013752:Tetracycline; D008751:Methylene Blue