title: Cryopreservation of in vitro grown nodal segments of Rauvolfia serpentina by PVS2 vitrification.
abstract: This paper describes the cryopreservation by PVS2 vitrification of Rauvolfia serpentina (L.) Benth ex kurz, an important tropical medicinal plant. The effects of type and size of explants, sucrose preculture (duration and concentration) and vitrification treatment were tested. Preliminary experiments with PVS1, 2 and 3 produced shoot growth only for PVS2. When optimizing the PVS2 vitrification of nodal segments, those of 0.31 - 0.39 cm in size were better than other nodal sizes and or apices. Sucrose preculture had a positive role in survival and subsequent regrowth of the cryopreserved explants. Seven days on 0.5 M sucrose solution significantly improved the viability of nodal segments. PVS2 incubation for 45 minutes combined with a 7-day preculture gave the optimum result of 66 percent. Plantlets derived after cryopreservation resumed growth and regenerated normally.
authors.affiliation: Department of Botany, Bose Institute, West Bengal, India. avikray19@rediffmail.com
authors.forename: Avik
authors.initials: A
authors.lastname: Ray
authors.forename: Sabita
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011926:Rauwolfia / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
chemical_list: D013395:Sucrose