title: Genome-wide identification of DNA-protein interactions using chromatin immunoprecipitation coupled with flow cell sequencing.
abstract: The transcriptional networks underlying mammalian cell development and function are largely unknown. The recently described use of flow cell sequencing devices in combination with chromatin immunoprecipitation (ChIP-seq) stands to revolutionize the identification of DNA-protein interactions. As such, ChIP-seq is rapidly becoming the method of choice for the genome-wide localization of histone modifications and transcription factor binding sites. As further studies are performed, the information generated by ChIP-seq is expected to allow the development of a framework for networks describing the transcriptional regulation of cellular development and function. However, to date, this technology has been applied only to a small number of cell types, and even fewer tissues, suggesting a huge potential for novel discovery in this field.
authors.affiliation: Department of Cancer Endocrinology, BC Cancer Research Center, 675 West 10th Avenue, Vancouver, BC, Canada.
authors.forename: Brad G
authors.initials: BG
authors.lastname: Hoffman
authors.forename: Steven J M
authors.initials: SJ
authors.lastname: Jones
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D011485:Protein Binding; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004268:DNA-Binding Proteins