title: Analysis of complex oligosaccharides using graphitized carbon liquid chromatography/mass spectrometry.
abstract: The sugar fraction of alpha-1 acid glycoprotein (AGP) was studied using porous graphitized carbon (PGC) chromatography coupled to mass spectrometry. Electrospray ionization provides efficient control over fragmentation; at low collision energy only molecular species were observed, allowing accurate oligosaccharide profiling. PGC chromatography was useful separating 18 sugars differing in monosaccharide composition. Most of these were separated into several isomeric forms; altogether 49 different oligosaccharides were found in AGP.
authors.affiliation: Hungarian Academy of Sciences, Chemical Research Center, H-1025, Pusztaszeri 59-67, Budapest, Hungary.
authors.forename: Lívia
authors.initials: L
authors.lastname: Budai
authors.forename: Ferenc
authors.initials: F
authors.lastname: Pollreisz
authors.forename: Olivér
authors.initials: O
authors.lastname: Ozohanics
authors.forename: Krisztina
authors.initials: K
authors.lastname: Ludányi
authors.forename: László
authors.initials: L
authors.lastname: Drahos
authors.forename: Károly
authors.initials: K
authors.lastname: Vékey
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006108:Graphite; D009844:Oligosaccharides / Q000032:analysis*; D009961:Orosomucoid / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009844:Oligosaccharides; D009961:Orosomucoid; D006108:Graphite