Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection.
We describe a procedure for quantification of vitamin K(1) in human plasma by HPLC. Samples, enriched with a vitamin K derivative as internal standard, were deproteinized, purified on polymeric RP-SPE cartridges and injected into HPLC equipped with a post-column on-line zinc metal reactor and a fluorometric detector. Median level in blood donors (n=87) was 1.967 nmol/L (0.93-4.01, 5th-95th percentiles), with a significant correlation between plasma levels and age (r=0.276, p=0.00958) and a lower (not significant) value in women than in men. This method, easy-to-handle and with a high throughput, can be used to identify covert states of vitamin K intake deficiency in patients thus at risk of alterations in blood clotting or bone mineralization.
Paroni
Rita
R
Department of Medicine, Surgery and Dentistry, University of Milan, Milan, Italy. rita.paroni@unimi.it
Faioni
Elena Maria
EM
Razzari
Cristina
C
Fontana
Gessica
G
Cattaneo
Marco
M
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005453:Fluorescence; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods*; D010837:Vitamin K 1 / Q000097:blood*
D010837:Vitamin K 1