title: Ellagitannin (BJA3121), an anti-proliferative natural polyphenol compound, can regulate the expression of MiRNAs in HepG2 cancer cells.
abstract: MicroRNAs (miRNAs) play an important role in cancers. A number of miRNA expression-profiling studies have been done to identify the miRNA signatures of cancers from different cellular origin. There is, however, relatively little information on how anticancer agents regulate miRNA expression. Ellagitannin (BJA3121), 1,3-Di-O-galloyl-4,6-(s)-HHDP-b-D-glucopyranose, is a new natural polyphenol compound isolated from Balanophora Japonica MAKINO. Our preliminary results have shown that BJA3121 had antiproliferative effect and modified the expression of different genes in human HepG(2) cancer cells. In this study, we further evaluate whether this antineoplastic compound is able to alter miRNA expression in HepG(2) cells. We demonstrated for the first time that BJA3121 can regulate the expression of 25 miRNAs, including 17 upregulated and 8 downregulated miRNAs in HepG(2) cells. Our results suggested that BJA3121-modifed miRNA expression can mediate, at least in part, the antiproliferative and multigene regulatory action induced by the compound on HepG(2) cancer cells.
authors.affiliation: School of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Wen
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Li
authors.forename: Zhong-qiu
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Jia-Jie
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Guang-Fa
authors.initials: GF
authors.lastname: Wang
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D031111:Balanophoraceae / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005419:Flavonoids / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D035683:MicroRNAs / Q000378:metabolism*; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols
chemical_list: D005419:Flavonoids; D047348:Hydrolyzable Tannins; D035683:MicroRNAs; D010636:Phenols; D059808:Polyphenols; C013515:ellagitannin