In vitro antimicrobial effect of bacteriocin PsVP-10 in combination with chlorhexidine and triclosan against Streptococcus mutans and Streptococcus sobrinus strains.
OBJECTIVE
To determine the minimal inhibitory concentrations (MICs) of bacteriocin PsVP-10, chlorhexidine and triclosan on S. mutans and S. sobrinus and to study the potential synergistic combination between these antimicrobial and the bacteriocin PsVP-10.


METHODS
Were determined MICs of bacteriocin PsVP-10, triclosan and chlorhexidine on strains of S. mutans and S. sobrinus, which formed a biofilm or did not form a biofilm. In addition, the synergistic effect was analysed by the determination of respective fractionary inhibitory concentrations (FICs) between bacteriocin PsVP-10 plus chlorhexidine and bacteriocin PsVP-10 plus triclosan.


RESULTS
MICs of three antimicrobials used were higher in those bacterial strains, which formed a biofilm. An interesting synergistic effect on both studied species was observed when bacteriocin and chlorhexidine were combined. A slighter synergy was determined for the combination bacteriocin PsVP-10 and triclosan.


CONCLUSIONS
The results showed that the combination of chlorhexidine bacteriocin PsVP-10 could reduce the number of cariogenic bacteria for in vitro studies. In the future this synergistic combination could be an alternative to antimicrobial therapy against S. mutans or S. sobrinus.
Lobos
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Departamento de Microbiología, Universidad de Talca, Camino Lircay s/n, Talca, Chile.
Padilla
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Padilla
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D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002648:Child; D002710:Chlorhexidine / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000188:drug therapy / Q000382:microbiology; D004357:Drug Synergism; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development; D016986:Streptococcus sobrinus / Q000187:drug effects* / Q000254:growth & development; D014260:Triclosan / Q000494:pharmacology*; D055815:Young Adult
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; C483835:PsVP-10 peptide, Pseudomonas; D014260:Triclosan; D002710:Chlorhexidine