Estimating population haplotype frequencies from pooled DNA samples using PHASE algorithm.
Recent studies show that the PHASE algorithm is a state-of-the-art method for population-based haplotyping from individually genotyped data. We present a modified version of PHASE for estimating population haplotype frequencies from pooled DNA data. The algorithm is compared with (i) a maximum likelihood estimation under the multinomial model and (ii) a deterministic greedy algorithm, on both simulated and real data sets (HapMap data). Our results suggest that the PHASE algorithm is a method of choice also on pooled DNA data. The main reason for improvement over the other approaches is assumed to be the same as with individually genotyped data: the biologically motivated model of PHASE takes into account correlated genealogical histories of the haplotypes by modelling mutations and recombinations. The important questions of efficiency of DNA pooling as well as influence of the pool size on the accuracy of the estimates are also considered. Our results are in line with the earlier findings in that the pool size should be relatively small, only 2-5 individuals in our examples, in order to provide reliable estimates of population haplotype frequencies.
Pirinen
Matti
M
Department of Mathematics and Statistics, University of Helsinki, PO Box 68, FIN-00014 University of Helsinki, Finland. matti.pirinen@helsinki.fi
Kulathinal
Sangita
S
Gasbarra
Dario
D
Sillanpää
Mikko J
MJ
D000465:Algorithms; D000483:Alleles; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D030561:Databases, Nucleic Acid; D004171:Diploidy; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D016013:Likelihood Functions; D008390:Markov Chains; D008957:Models, Genetic; D009010:Monte Carlo Method
D004247:DNA