title: User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.
abstract: We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.
authors.affiliation: Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Schroeder
authors.forename: Michael
authors.initials: M
authors.lastname: Adler
authors.forename: Katrin
authors.initials: K
authors.lastname: Gerigk
authors.forename: Birgit
authors.initials: B
authors.lastname: Müller-Chorus
authors.forename: Friedrich
authors.initials: F
authors.lastname: Götz
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D040081:Protein Array Analysis
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D004247:DNA