[A gene chip study of "Jun Du Yan Bingzhi" on liver in a sepsis model of rat].
OBJECTIVE
To study the effect of "Jun Du Yan Bingzhi" on genic change in liver of a sepsis rat model by gene chip technique, in order to study the mechanism of the action of the drug on the gene level.


METHODS
Ninety rats were randomly divided into normal control group, model group and "Jun Du Yan Bingzhi" group, with 30 rats in each group. Sepsis was reproduced by cecal ligation and puncture (CLP) method. In "Jun Du Yan Bingzhi" group the rats were treated with intraperitoneal injection of imipenem/cilastatin (0.18 g/kg), Xuebijing injection (10 ml/kg) and gavage of "Liangge San" (15 ml/kg). In the control group and model group intraperitoneal physiological saline (10 ml/kg) was given; Survival time, and 48-hour and 72-hour survival rates of every group were observed, and changes in liver genes were examined with BiostarR-40 s chip. The ratio of Cy3/Cy5 > or =2.0 or < or =0.5 was used to screen differential genes, and NCBI database was used to identity the function of differential genes.


RESULTS
The 48-hour and 72-hour survival rate of "Jun Du Yan Bingzhi" group was significantly higher than that of model group (83.3% vs. 30.0%, 76.7% vs. 17.7%, both P<0.01), 305 differential genes were found in model/control groups, with up-regulation in 159, down-regulation in 146, 500 differential genes were found in "Jun Du Yan Bingzhi" group/model group, with up-regulation in 292, down-regulation in 208, model group/control group up-regulation and "Jun Du Yan Bingzhi" group/model group down-regulation were 48, model group/control group down-regulation and "Jun Du Yan Bingzhi" group/model group up-regulation were 63.


CONCLUSIONS
"Jun Du Yan Bingzhi" can degrade the 48-hour and 72-hour death rate of sepsis rat, through control immunization related, inflammation, signal transduction transcription regulation, cell cycle, apoptosis, substance metabolism, translation/processing/modify/degradation of protein, differentiation/proliferation/growth of cell related gene, promote multisystem function of sepsis rat to recover normal.
Li
Zhi-jun
ZJ
The First Central Hospital of Tianjin, Tianjin 300192, China. doctorzhijun@yahoo.cn
Wang
Dong-qiang
DQ
Hu
Shun-peng
SP
Wang
Xiao-fei
XF
He
Ying
Y
Zhang
Zhao-jian
ZJ
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000188:drug therapy* / Q000235:genetics*
D004365:Drugs, Chinese Herbal