Alkali-soluble polysaccharides of Rhizoclonium riparium alga induce IL-1 gene expression via protein kinase signaling pathways.
Fortification of aquaculture foodstuff with various algae may improve the resistance of certain fish or shrimp to diseases and, as a routine procedure, has become ever more popular and, seemingly, important. Herein, we isolated certain alkali-soluble polysaccharides from a Rhizoclonium riparium alga (RASP), polysaccharides that can be separated into two different groups on the basis of the polysaccharide's molecular weight. Using gas chromatography-mass spectometry analysis, we found that the major monosaccharides constituting the higher molecular-weight group of RASP were galactose (41.99%), glucose (34.53%), xylose (20.24%), and mannose (3.24%). Using a murine-derived macrophage cell line J774A.1, we found that polysaccharide constituents of the higher molecular-weight group of RASP were able to induce interleukin-1beta (IL-1) gene expression via protein kinase-mediated signal transduction pathways. In essence, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38), but not extracellular signal-regulated kinase (ERK), play an important role in the regulation of IL-1 gene expression in RASP-stimulated J774A.1 cells. To the best of our knowledge, this is the first occasion that polysaccharides from R. riparium have been demonstrated to exert immunomodulation properties by the induction of IL-1 within macrophages. Our current results provide support for the possible use of R. riparium as an additive to various food/foodstuff, to modulate the immune response of humans or certain animals.
Hsu
Hsien-Yeh
HY
Faculty of Biotechnology and Laboratory Science in Medicine, Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, Taiwan.
Hua
Kuo-Feng
KF
Su
Yu-Chang
YC
Chu
Li-Chieh
LC
Su
Shi-Chi
SC
Chiu
Huan-Wen
HW
Wong
Chi-Huey
CH
Chen
Shui-Tein
ST
Shieh
Chu-Wen
CW
Yang
Su-Sing
SS
Chen
Ya-Mai
YM
Chao
Louis Kuoping
LK
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D056890:Eukaryota / Q000737:chemistry; D005527:Food, Fortified; D015870:Gene Expression / Q000187:drug effects*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000276:immunology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction
D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D011134:Polysaccharides; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases