Growth and adipogenic differentiation of mesenchymal stromal bone marrow cells during culturing in 3D macroporous agarose cryogel sponges.
We studied the possibility of population of macroporous agarose cryogel sponges by mesenchymal stromal bone marrow cells with their subsequent adipogenic differentiation. After 7-day culturing of mesenchymal stromal cells in agarose cryogel, the level of cell proliferation was 35%. After 3-week culturing in a medium inducing adipogenesis we observed accumulation of intracellular neutral lipids positively stained with Oil Red O. These findings can be used for the development of bioengineering constructions of the adipose tissue on the basis of spongy carriers.
Petrenko
Yu A
YA
Institute of Cryobiology and Cryomedicine Problems, National Academy of Sciences of Ukraine, Khar'kov. yuripetrenko@cryo.org.ua
Petrenko
A Yu
AY
Damshkaln
L G
LG
Volkova
N A
NA
Lozinsky
V I
VI
D050156:Adipogenesis / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D059985:Cryogels; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D012685:Sepharose / Q000737:chemistry / Q000378:metabolism
D001672:Biocompatible Materials; D001798:Blood Proteins; D059985:Cryogels; D005353:Fibronectins; D020100:Hydrogels; D012685:Sepharose