title: agr receptor mutants reveal distinct modes of inhibition by staphylococcal autoinducing peptides.
abstract: Through the agr quorum-sensing system, staphylococci secrete unique autoinducing peptides (AIPs) and detect their concentration via the AgrC transmembrane receptor, coordinating local bacterial population density with global changes in gene expression. Unique AIP and AgrC variants exist within and between species, and although autologous interactions lead to agr activation, heterologous interactions usually lead to cross-inhibition, resulting in natural quorum-sensing interference. To gain insight into the mechanisms responsible for these phenomena at the level of the receptor, we used random mutagenesis to isolate variants of Staphylococcus aureus AgrC-I with constitutive activity. Constitutive mutations in the sensor domain of the receptor were localized to the last transmembrane helix, whereas those in the histidine kinase domain were mostly clustered to a region near the phosphorylation site histidine. Analysis of these mutants with a range of noncognate AIPs revealed that inhibition is manifested by inverse agonism in certain heterologous pairings and by neutral antagonism in others. In addition, we isolated and characterized an AgrC sensor domain mutant with dramatically broadened activation specificity and reduced sensitivity to inhibition, identifying a single amino acid as a critical determinant of ligand-mediated inhibition. These results suggest that certain noncognate AIPs stabilize an inhibitory receptor conformation that may be a critical feature of the ligand-receptor interaction not initially appreciated in previous analyses of agr inhibition.
authors.affiliation: Department of Medicine, Kimmel Center for Biology and Medicine of the Skirball Institute, New York University Medical Center, New York, NY 10016, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Geisinger
authors.forename: Tom W
authors.initials: TW
authors.lastname: Muir
authors.forename: Richard P
authors.initials: RP
authors.lastname: Novick
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000302:isolation & purification / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D010455:Peptides / Q000494:pharmacology*; D010456:Peptides, Cyclic; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012641:Selection, Genetic; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: C414532:AgrD protein, Staphylococcus; D000596:Amino Acids; D001426:Bacterial Proteins; D050505:Mutant Proteins; D010455:Peptides; D010456:Peptides, Cyclic; D011494:Protein Kinases; D000071677:Histidine Kinase; C531166:AgrC protein, Staphylococcus