title: Klebsiella pneumoniae LO10 producing extended-spectrum beta-lactamase SHV-12 in Saudi Arabia.
abstract: Klebsiella pneumoniae LO10 was responsible for an outbreak that occurred in the neonatal unit at Security Forces Hospital, Kingdom of Saudi Arabia. Over a period of eight months nine cases of bacteremia resulted in two deaths. Resistance to third generation cephalosporins was transferred from strain LO10 to E. coli by both conjugation and transformation. Sequence determination of the plasmid gene from two transconjugants and one transformant indicated that resistance was carried by a ca.100-kb plasmid encoding beta-lactamase SHV-12. This is the first description of a K. pneumoniae producing a type SHV-12 extended spectrum beta-lactamase in Riyadh. Long term exposure to antibiotics, prolonged stay, and heavy use of third generation cephalosporins contributed to the spread of the resistant strain in the unit. Strict infection control measures led to control of the outbreak.
authors.affiliation: Department of Medical Laboratory and Blood Bank, Security Forces Hospital Program, Riyadh, Kingdom of Saudi Arabia. obeid_Jacques@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: Al-Obeid
authors.forename: S
authors.initials: S
authors.lastname: Bremont
authors.forename: L
authors.initials: L
authors.lastname: Jabri
authors.forename: N
authors.initials: N
authors.lastname: Massoudi
authors.forename: Q
authors.initials: Q
authors.lastname: Haddad
mesh_terms: D016470:Bacteremia / Q000382:microbiology; D003428:Cross Infection / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007231:Infant, Newborn; D007710:Klebsiella Infections / Q000201:enzymology / Q000453:epidemiology* / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D012529:Saudi Arabia / Q000453:epidemiology; D018805:Sepsis / Q000382:microbiology; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: C107198:beta-lactamase SHV-12; D001618:beta-Lactamases