title: In guinea pig sperm, aldolase A forms a complex with actin, WAS, and Arp2/3 that plays a role in actin polymerization.
abstract: Glycolytic enzymes have, in addition to their role in energy production, other functions in the regulation of cellular processes. Aldolase A has been reported to be present in sperm, playing a key role in glycolysis; however, despite its reported interactions with actin and WAS, little is known about a non-glycolytic role of aldolase A in sperm. Here, we show that in guinea pig spermatozoa, aldolase A is tightly associated to cytoskeletal structures where it interacts with actin, WAS, and Arp2/3. We show that aldolase A spermatozoa treatment increases their polymerized actin levels. In addition, we show that there is a direct correlation between the levels of polymerized actin and the levels of aldolase A-actin interaction. Our results suggest that aldolase A functions as a bridge between filaments of actin and the actin-polymerizing machinery.
authors.affiliation: Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), PC07360 México DF, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Chiquete-Felix
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Hernández
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Méndez
authors.forename: Armando
authors.initials: A
authors.lastname: Zepeda-Bastida
authors.forename: Alicia
authors.initials: A
authors.lastname: Chagolla-López
authors.forename: Adela
authors.initials: A
authors.lastname: Mújica
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D058105:Polymerization; D013094:Spermatozoa / Q000201:enzymology*; D051316:Wiskott-Aldrich Syndrome Protein / Q000378:metabolism*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D051316:Wiskott-Aldrich Syndrome Protein; D005634:Fructose-Bisphosphate Aldolase