Surfactin blocks NO production in lipopolysaccharide-activated macrophages by inhibiting NF-kappaB activation.
Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin downregulated LPSinduced NO production in RAW264.7 cells and primary macrophages with IC50 values of 31.6 and 22.4 microM, respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and IkBa and the nuclear translocation of NF-kappaB (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived antiinflammatory agent with anti-NF-kappaB activity.
Byeon
Se Eun
SE
School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Lee
Yong Gyu
YG
Kim
Byung Hun
BH
Shen
Ting
T
Lee
Sang Yeol
SY
Park
Hwa Jin
HJ
Park
Seung-Chun
SC
Rhee
Man Hee
MH
Cho
Jae Youl
JY
D000704:Analysis of Variance; D000818:Animals; D001412:Bacillus subtilis / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D055666:Lipopeptides / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology*
D016207:Cytokines; D004791:Enzyme Inhibitors; D055666:Lipopeptides; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010456:Peptides, Cyclic; C009365:surfactin peptide; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II