title: Identification of plasmid- and integron-borne blaIMP-1 and blaIMP-10 in clinical isolates of Serratia marcescens.
abstract: The emergence of carbapenem-hydrolysing metallo-beta-lactamases (MBLs) is a serious threat to the clinical utility of carbapenems. This study identified plasmid- and integron-borne bla(IMP-1) and bla(IMP-10) in clinical isolates of Serratia marcescens. The bla(IMP-1) and bla(IMP-10) gene cassettes were carried by a class 1 integron and followed by the aac(6')-IIc gene cassette. The bla(IMP-1) and bla(IMP-10) gene cassettes were preceded by a weak P(ant) promoter, TGGACA(N)(17)TAAGCT, and an inactive P2 promoter, TTGTTA(N)(14)TACAGT. These genes were easily transferred to Escherichia coli by conjugation and transformation, indicating that they are located on transferable plasmids. Due to the acquisition of bla(IMP-1), the susceptibility of E. coli transconjugants to imipenem, meropenem, panipenem and biapenem decreased by 32-, 256-, 64- and 128-fold, respectively. In comparison, after gaining bla(IMP-10), the susceptibility of E. coli transconjugants to the four carbapenems decreased by 64-, 2048-, 256- and 64-fold, respectively. Strains harbouring bla(IMP-10) showed higher-level resistance to imipenem, meropenem and panipenem than the strains harbouring bla(IMP-1), although the nucleotide sequences of the class 1 integrons carrying bla(IMP-10) and bla(IMP-1) were identical except for a single point mutation.
authors.affiliation: Department of Biology, The College of Liberal Arts, International Christian University, Tokyo 181-8585, Japan.
authors.forename: Zhuting
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Microbiology and Immunology, Showa University School of Medicine, Tokyo 142-8555, Japan.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D022761:Gene Transfer, Horizontal; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D011401:Promoter Regions, Genetic; D016868:Serratia Infections / Q000382:microbiology; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C409201:beta-lactamase IMP-1; C466680:metallo-beta-lactamase, IMP-10; D001618:beta-Lactamases