Comparative study between intact and non-intact intramuscular auto-grafted mouse ovaries.
Anticancer treatments often lead to ovarian failure and infertility. Cryopreservation and subsequent transplantation of the ovaries is one of the solutions that has been adopted as a means of preserving fertility, but primitive ischaemia in the grafted ovary that damages the oocyte pool is considered to be a possible problem. In order to improve blood supply and follicle preservation, two incisions were made in the ovaries before an intramuscular auto-grafting procedure and these non-intact ovaries were compared with the intact ovaries that were also auto-grafted intramuscularly. Follicle numbers and apoptosis were examined in intact and non-intact groups after 1, 2 and 3 weeks post-grafting. The results were compared with the control ovaries, which were not incised and grafted. Although follicle survival in both grafted groups was lower than in the controls (P <or= 0.05), survival of follicles in the grafted ovaries (n = 19) was improved by incision prior to grafting. In addition, the estimated number of follicles decreased in grafted ovaries compared with non-grafted ovaries. Generally, it seems that this procedure is a promising method to preserve ovarian function; further studies are required to improve the success of maintaining ovaries after transplant.
Eimani
Hussein
H
Embryology Department, Reproductive Medicine Research Center, Royan Institute, ACECR, Tehran, Iran. eimani@yahoo.com
Siadat
Saiedeh Fatemeh
SF
Eftekhari-Yazdi
Poopak
P
Parivar
K
K
Rezazadeh Valojerdi
Mojtaba
M
Shahverdi
Abdolhossein
A
D000818:Animals; D019610:Cytoprotection / Q000502:physiology; D053938:DNA Fragmentation; D005260:Female; D051379:Mice; D018482:Muscle, Skeletal; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000293:injuries* / Q000378:metabolism / Q000637:transplantation*; D020127:Recovery of Function; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic / Q000379:methods*