Sindbis virus-based measles DNA vaccines protect cotton rats against respiratory measles: relevance of antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and Th1-type cytokines as correlates of immunity.
Measles remains an important cause of pediatric morbidity and mortality in developing countries, especially among infants who are too young to receive the current licensed live attenuated measles vaccine. We developed two Sindbis virus DNA vaccines encoding the measles virus hemagglutinin (pMSIN-H) and fusion proteins (pMSINH-FdU) and examined their immunogenicities and protective efficacies when administered alone or followed by the live measles virus vaccine in cotton rats. Neutralizing antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and gamma interferon-secreting T cells developed after priming and increased after boosting. pMSIN-H priming conferred 100% protection against pulmonary measles, whereas pMSINH-FdU protected only in conjunction with the live measles virus vaccine boost.
Pasetti
Marcela F
MF
Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Room 480, Baltimore, Maryland 21201, USA. mpasetti@medicine.umaryland.edu
Ramirez
Karina
K
Resendiz-Albor
Aldo
A
Ulmer
Jeffrey
J
Barry
Eileen M
EM
Levine
Myron M
MM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000276:immunology; D006801:Humans; D018928:Immunity, Mucosal; D007117:Immunization, Secondary; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000821:virology; D008457:Measles / Q000517:prevention & control*; D008458:Measles Vaccine / Q000235:genetics / Q000276:immunology*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D051381:Rats; D016205:Sigmodontinae; D012845:Sindbis Virus / Q000235:genetics*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D016207:Cytokines; D006389:Hemagglutinins, Viral; D008458:Measles Vaccine; D019444:Vaccines, DNA; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus; D007371:Interferon-gamma