PBX1 is dispensable for neural commitment of RA-treated murine ES cells.
Experimentation with PBX1 knockout mice has shown that PBX1 is necessary for early embryogenesis. Despite broad insight into PBX1 function, little is known about the underlying target gene regulation. Utilizing the Cre-loxP system, we targeted a functionally important part of the homeodomain of PBX1 through homozygous deletion of exon-6 and flanking intronic regions leading to exon 7 skipping in embryonic stem (ES) cells. We induced in vitro differentiation of wild-type and PBX1 mutant ES cells by aggregation and retinoic acid (RA) treatment and compared their profiles of gene expression at the ninth day post-reattachment to adhesive media. Our results indicate that PBX1 interactions with HOX proteins and DNA are dispensable for RA-induced ability of ES to express neural genes and point to a possible involvement of PBX1 in the regulation of imprinted genes.
Jürgens
Anne S
AS
Department of Medicine I, University of Freiburg Medical Center, Hugstetter Str. 55, 79106, Freiburg, Germany.
Kolanczyk
Mateusz
M
Moebest
Dietrich C C
DC
Zemojtel
Tomasz
T
Lichtenauer
Urs
U
Duchniewicz
Marlena
M
Gantert
Melanie P
MP
Hecht
Jochen
J
Hattenhorst
Uwe
U
Burdach
Stefan
S
Dorn
Annette
A
Kamps
Mark P
MP
Beuschlein
Felix
F
Räpple
Daniel
D
Scheele
Jürgen S
JS
D000483:Alleles; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004475:Ectoderm / Q000187:drug effects / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018390:Gene Targeting; D018392:Genomic Imprinting / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D019426:Integrases / Q000378:metabolism; D007438:Introns / Q000235:genetics; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D011995:Recombination, Genetic / Q000187:drug effects; D017384:Sequence Deletion / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*
D018398:Homeodomain Proteins; D050505:Mutant Proteins; C481797:Pbx1 protein, mouse; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D014157:Transcription Factors; D014212:Tretinoin; C045073:Cre recombinase; D019426:Integrases