title: Pilot study of oxygen transport rate of banked red blood cells.
abstract: OBJECTIVE
  Dynamic oximetry provides a new way to assess the effect of blood storage on the oxygen transport rate (OTR).
  
  
  METHODS
  In dynamic oximetry, the rate at which oxyhemoglobin becomes deoxyhemoglobin is measured optically, thereby, indirectly measuring the rate at which oxygen leaves the red blood cell (RBC) making it available for transfer to tissues. Extending the physiologic diffusion time in an in vitro apparatus, consisting of a diffusion system and gas exchanger capable of controlling the surface area and the time of exposure for oxygenation and deoxygenation, makes OTR measurement feasible. Eight normal blood donor units, collected in adenine, dextrose, sorbitol, sodium chloride and mannitol , were stored for 8 weeks under standard conditions and serially sampled for OTR.
  
  
  RESULTS
  We report that the OTR at the time of blood bank donation appears to be singular for each donor, that the interdonor differences are maintained over time, and that the individual OTR increased 1.72-fold (95% CI 1.51, 1.95) over 8 weeks, adjusting for sex, age and plasma cholesterol level.
  
  
  CONCLUSIONS
  Oxygen transport rate increases during storage; blood units with similar haemoglobin content may have significant differences in OTR. Studies examining blood parameters at the time of donation and blood storage on patient outcomes should consider measuring OTR, as it may contribute to differences in observed efficacy of tissue oxygenation.
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis, USA. buchw001@umn.edu
authors.forename: H
authors.initials: H
authors.lastname: Buchwald
authors.forename: H J
authors.initials: HJ
authors.lastname: Menchaca
authors.forename: V N
authors.initials: VN
authors.lastname: Michalek
authors.forename: K D
authors.initials: KD
authors.lastname: Rudser
authors.forename: T D
authors.initials: TD
authors.lastname: Rohde
authors.forename: T
authors.initials: T
authors.lastname: O'Dea
authors.forename: J E
authors.initials: JE
authors.lastname: Connett
authors.forename: J
authors.initials: J
authors.lastname: Gorlin
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000328:Adult; D001273:Atmosphere Exposure Chambers; D001692:Biological Transport; D001793:Blood Preservation / Q000379:methods; D002784:Cholesterol / Q000097:blood; D004058:Diffusion; D004867:Equipment Design; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008875:Middle Aged; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010092:Oximetry / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000008:administration & dosage / Q000097:blood*; D010108:Oxyhemoglobins / Q000032:analysis; D010865:Pilot Projects; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D055815:Young Adult
chemical_list: D006454:Hemoglobins; D019188:Organ Preservation Solutions; D010108:Oxyhemoglobins; D008353:Mannitol; D012965:Sodium Chloride; D013012:Sorbitol; C100003:deoxyhemoglobin; D002784:Cholesterol; D005947:Glucose; D000225:Adenine; D010100:Oxygen