Induction of macroautophagy by heat.
Macroautophagy is a regulated bulk degradation process of cellular components, mainly long-lived proteins or cytoplasmic organelles. Nutrient depletion is a classic inducer of macroautophagy. In this report, we have induced heat-mediated macroautophagy in several cell lines in the absence of nutrient depletion. Heat treatment increased the autophagic markers LC3-I and LC3-II at the protein levels. Interestingly, expression of a constitutively active HSF1 mutant suppressed basal LC3-II protein level and heat-induced increase of LC3-II. Our results provide evidence that heat is a potent inducer of macroautophagy in mammalian cells, and implicate the negative role of active HSF1 in this process.
Zhao
Yuanbo
Y
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Science Park, 510663 Guangzhou, China.
Gong
Soufang
S
Shunmei
E
E
Zou
Jiangying
J
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D014157:Transcription Factors