A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells.
Insect cell expression systems are widely used to produce active recombinant proteins. Here, we have developed a high-level expression vector containing a selectable marker for continuous production of recombinant proteins in insect cells. The plasmid, pXIHAbla, developed in this study, established a polyclonal cell line 8 days shorter than pXINSECT-DEST38 and pBmAneo. In addition, pXIHAbla exhibited an approximately fivefold higher average enhanced GFP expression level and approximately a twofold higher bionanocapsule secretion level than pXINSECT-DEST38. Using this plasmid, insect cells that highly express active proteins have been easily established.
Shishido
Takuya
T
Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada-Ku, Kobe, 657-8501, Japan.
Kurata
Naoya
N
Yoon
Myung Eui
ME
Tanaka
Tsutomu
T
Yamaji
Hideki
H
Fukuda
Hideki
H
Kondo
Akihiko
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D004351:Drug Resistance; D015870:Gene Expression; D005822:Genetic Vectors; D007313:Insecta; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012641:Selection, Genetic
D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins