Constitutive repression and activation of auxin signaling in Arabidopsis.
Aux/IAA proteins are proposed to be transcriptional repressors that play a crucial role in auxin signaling by interacting with auxin response factors and repressing early/primary auxin response gene expression. In assays with transfected protoplasts, this repression was previously shown to occur when auxin concentrations in a cell are low, and derepression/activation was observed when auxin concentrations are elevated. Here we show that a stabilized version of the Arabidopsis (Arabidopsis thaliana) IAA17 repressor, when expressed constitutively or in a specific cell type in Arabidopsis plants, confers phenotypes similar to plants with decreased auxin levels. In contrast, a stabilized version of IAA17 that was converted to a transcriptional activator confers phenotypes similar to plants with increased auxin levels, when expressed under the same conditions in Arabidopsis plants. Free auxin levels were unchanged compared to control (DR5:beta-glucuronidase), however, in the seedlings expressing the IAA17 repressor and activator. These results together with our previous results carried out in transfected protoplasts suggest that the hormone auxin can be bypassed to regulate auxin signaling in a cell-autonomous manner in plants.
Li
Hanbing
H
Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, USA.
Cheng
Yan
Y
Murphy
Angus
A
Hagen
Gretchen
G
Guilfoyle
Tom J
TJ
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000378:metabolism; D017405:Herpes Simplex Virus Protein Vmw65 / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D009687:Nuclear Proteins / Q000737:chemistry; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D055550:Protein Stability / Q000187:drug effects; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014157:Transcription Factors; D019076:Transgenes
C110728:AXR3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D017405:Herpes Simplex Virus Protein Vmw65; D007210:Indoleacetic Acids; D009687:Nuclear Proteins; D010940:Plant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C095825:expansin protein, plant; D005966:Glucuronidase