title: Interaction of Epstein-Barr virus BZLF1 C-terminal tail structure and core zipper is required for DNA replication but not for promoter transactivation.
abstract: The Epstein-Barr virus (EBV) protein BZLF1 contains a bZIP DNA binding domain in which C-terminal tail residues fold back against a zipper region that forms a coiled coil and mediates dimerization. Point mutagenesis in the zipper region reveals the importance of individual residues within the (208)SSENDRLR(215) sequence that is conserved in C/EBP for transactivation and EBV DNA replication. The restoration of BZLF1 DNA replication activity by the complementation of two deleterious mutations (S208E and D236K) indicates that the interaction of the C-terminal tail and the core zipper is required for DNA replication, identifying a functional role for this structural feature unique to BZLF1.
authors.affiliation: Department of Virology, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom.
authors.forename: Carol M
authors.initials: CM
authors.lastname: McDonald
authors.forename: Carlo
authors.initials: C
authors.lastname: Petosa
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Farrell
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D002460:Cell Line; D004261:DNA Replication; D019281:Dimerization; D005816:Genetic Complementation Test; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; D015534:Trans-Activators