title: Characterization of Capsicum annuum recombinant alpha- and beta-tubulin.
abstract: There are several conditions which might modulate polymerization to produce polymers having normal lattice structure. In the absence of 1 mM MgCl(2) the assembly was reduced by 36% in Capsicum annuum tubulin (CAnm tubulin). There was no significant difference in the final assembly formation in the presence of 5% to 10% glycerol. However, nucleation rate was slow and apparent study state was achieved lately in the presence of 10% glycerol. Taxol at 100 microM concentration increased 23% tubulin assembly. One millimolar CaCl(2), >or=1% dimethyl sulfoxide (DMSO) and physiologically low temperature reduced CAnm tubulin assembly. A value of 0.089 mg/ml was obtained as critical concentration for polymerization. Benomyl significantly reduced the number of cysteine residues accessible to 5,5'-dithiobis-(2-nitrobenzoic acid); there were 4.77 +/- 0.21 and 3.49 +/- 0.35 residues accessible per tubulin dimer in the presence of 50 and 100 microM benomyl respectively.
authors.affiliation: Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon, Korea.
authors.forename: Bon-Sung
authors.initials: BS
authors.lastname: Koo
authors.forename: Myung-Hyun
authors.initials: MH
authors.lastname: Jang
authors.forename: Haechul
authors.initials: H
authors.lastname: Park
authors.forename: Satish
authors.initials: S
authors.lastname: Kalme
authors.forename: Hey-Yeon
authors.initials: HY
authors.lastname: Park
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Han
authors.forename: Yun-Soo
authors.initials: YS
authors.lastname: Yeo
authors.forename: Sang-Hong
authors.initials: SH
authors.lastname: Yoon
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Chang-Muk
authors.initials: CM
authors.lastname: Lee
authors.forename: Moon-Young
authors.initials: MY
authors.lastname: Yoon
mesh_terms: D002212:Capsicum; D004228:Dithionitrobenzoic Acid / Q000378:metabolism; D007202:Indicators and Reagents / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D055503:Protein Multimerization / Q000187:drug effects; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007202:Indicators and Reagents; D010940:Plant Proteins; D011994:Recombinant Proteins; D014404:Tubulin; D004228:Dithionitrobenzoic Acid