Endothelial nitric oxide synthase: from structure to function in one aspartic substitution.
Cherney and co-workers examine the functional phenotype of the G894T eNOS polymorphism in a selected group of apparently healthy subjects. The investigators used L-arginine infusion and induced changes in renal hemodynamics. While L-arginine increased effective plasma flow and glomerular filtration rate in GG subjects, these responses were blunted in GT/TT subjects, suggesting that either the G894T polymorphism impairs eNOS function, or GG subjects exhibit augmented eNOS responsiveness to L-arginine. The complexity of this seemingly straightforward conclusion deserves elaboration.
Goligorsky
Michael S
MS
Department of Medicine, Renal Research Institute, New York Medical College, Valhalla, New York 10595, USA. michael_goligorsky@nymc.edu
D019943:Amino Acid Substitution; D001120:Arginine / Q000494:pharmacology; D001224:Aspartic Acid / Q000378:metabolism*; D001665:Binding Sites; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011110:Polymorphism, Genetic; D011485:Protein Binding; D012079:Renal Circulation / Q000187:drug effects; D013329:Structure-Activity Relationship
D001224:Aspartic Acid; D009569:Nitric Oxide; D001120:Arginine; D052250:Nitric Oxide Synthase Type III