MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells.
MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between normal brain and glioma tissues from Chinese patients by miRNA microarray and identified a panel of differentially expressed miRNAs. Here, we studied the function of one miRNA, miR-15b, in glioma carcinogenesis and elucidated its downstream targets. Over-expression of miR-15b resulted in cell cycle arrest at G0/G1 phase while suppression of miR-15b expression resulted in a decrease of cell populations in G0/G1 and a corresponding increase of cell populations in S phase. We further showed that CCNE1 (encoding cyclin E1) is one of the downstream targets of miR-15b. Taken together, our findings indicate that miR-15b regulates cell cycle progression in glioma cells by targeting cell cycle-related molecules.
Xia
Hongping
H
Integrative Chemical Biology Laboratory, Institute of Molecular Technology, Department of Chemistry, 8/F, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
Qi
Yanting
Y
Ng
Samuel S
SS
Chen
Xiaona
X
Chen
Shen
S
Fang
Marong
M
Li
Dan
D
Zhao
Yu
Y
Ge
Ruiguang
R
Li
Guo
G
Chen
Yangchao
Y
He
Ming-Liang
ML
Kung
Hsiang-fu
HF
Lai
Lihui
L
Lin
Marie C
MC
D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D016213:Cyclins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
D016213:Cyclins; C505361:MIRN15 microRNA, human; D035683:MicroRNAs