title: Genetics and proteomics of Aeromonas salmonicida lipopolysaccharide core biosynthesis.
abstract: Comparison between the lipopolysaccharide (LPS) core structures of Aeromonas salmonicida subsp. salmonicida A450 and Aeromonas hydrophila AH-3 shows great similarity in the inner LPS core and part of the outer LPS core but some differences in the distal part of the outer LPS core (residues ld-Hep, d-Gal, and d-GalNAc). The three genomic regions encoding LPS core biosynthetic genes in A. salmonicida A450, of which regions 2 and 3 have genes identical to those of A. hydrophila AH-3, were fully sequenced. A. salmonicida A450 region 1 showed seven genes: three identical to those of A. hydrophila AH-3, three similar but not identical to those of A. hydrophila AH-3, and one without any homology to any well-characterized gene. A. salmonicida A450 mutants with alterations in the genes that were not identical to those of A. hydrophila AH-3 were constructed, and their LPS core structures were fully elucidated. At the same time, all the A. salmonicida A450 genes identical to those of A. hydrophila AH-3 were used to complement the previously obtained A. hydrophila AH-3 mutants for each of these genes. Combining the gene sequence and complementation test data with the structural data and phenotypic characterization of the mutant LPSs enabled a presumptive assignment of all LPS core biosynthesis gene functions in A. salmonicida A450. Furthermore, hybridization studies with internal probes for the A. salmonicida-specific genes using different A. salmonicida strains (strains of different subspecies or atypical strains) showed a unique or prevalent LPS core type, which is the one fully characterized for A. salmonicida A450.
authors.affiliation: Departamento de Microbiología, Universidad Barcelona, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Jimenez
authors.forename: Anna
authors.initials: A
authors.lastname: Lacasta
authors.forename: Silvia
authors.initials: S
authors.lastname: Vilches
authors.forename: Mercé
authors.initials: M
authors.lastname: Reyes
authors.forename: Judit
authors.initials: J
authors.lastname: Vazquez
authors.forename: Eleonora
authors.initials: E
authors.lastname: Aquillini
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.forename: Miguel
authors.initials: M
authors.lastname: Regué
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D048409:Aeromonas salmonicida / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002240:Carbohydrate Sequence; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides