title: Host-derived smooth muscle cells accumulate in cardiac allografts: role of inflammation and monocyte chemoattractant protein 1.
abstract: Transplant arteriosclerosis is characterized by inflammation and intimal thickening caused by accumulation of smooth muscle cells (SMCs) both from donor and recipient. We assessed the relationship between clinical factors and the presence of host-derived SMCs in 124 myocardial biopsies from 26 consecutive patients who received hearts from opposite-sex donors. Clinical and demographic information was obtained from the patients' medical records. Host-derived SMCs accounted for 3.35+/-2.3% of cells in arterioles (range, 0.08-12.51%). As shown by linear regression analysis, an increased number of SMCs was associated with rejection grade (mean, 1.41+/-1.03, p = 0.034) and the number of leukocytes (19.1+/-12.7 per 20 high-power fields, p = 0.01). The accumulation of host-derived SMCs was associated with an increased number of leukocytes in the allografts. In vitro, monocyte chemoattractant protein 1 (MCP-1) released from leukocytes was crucial for SMC migration. After heart allotransplantation, mice treated with MCP-1-specific antibodies had significantly fewer host-derived SMCs in the grafts than mice treated with isotypic antibody controls. We conclude that the number of host-derived SMCs in human cardiac allografts is associated with the rejection grade and that MCP-1 may play pivotal role in recruiting host-derived SMCs into cardiac allografts.
authors.affiliation: Cellular and Molecular Immunology, Karolinska Institute, Stockholm, Sweden. piotr.religa@ki.se
authors.forename: Piotr
authors.initials: P
authors.lastname: Religa
authors.forename: Monika K
authors.initials: MK
authors.lastname: Grudzinska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Bojakowski
authors.forename: Joanna
authors.initials: J
authors.lastname: Soin
authors.forename: Jerzy
authors.initials: J
authors.lastname: Nozynski
authors.forename: Michal
authors.initials: M
authors.lastname: Zakliczynski
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Gaciong
authors.forename: Marian
authors.initials: M
authors.lastname: Zembala
authors.forename: Cecilia
authors.initials: C
authors.lastname: Söderberg-Nauclér
mesh_terms: D000818:Animals; D001160:Arterioles; D018932:Chemokine CCL2 / Q000502:physiology*; D005260:Female; D006084:Graft Rejection; D016027:Heart Transplantation / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D007962:Leukocytes; D008297:Male; D051379:Mice; D009206:Myocardium / Q000473:pathology*; D032389:Myocytes, Smooth Muscle / Q000473:pathology*; D014184:Transplantation, Homologous
chemical_list: D018932:Chemokine CCL2