title: Genotyping of different Pseudomonas aeruginosa morphotypes arising from the lower respiratory tract of a patient taken to an Intensive Care Unit.
abstract: Pseudomonas aeruginosa is an opportunistic pathogen and an ubiquitous environmental bacterium. Fifty-seven days after hospitalization, we isolated three distinct P. aeruginosa morphotypes (smooth, rough and mucoid) from the lower respiratory tract of a patient admitted to a Cardiology Intensive Care Unit (ICU). Moreover, a group of nine colony variants, arising from the three P. aeruginosa isolates growing in laboratory growth media, were also isolated. The resulting 12 isolates were characterised for antibiotic resistance profile and subjected to genotypic analysis by fluorescent-Amplified Fragment Length Polymorphism (f-AFLP) and automated repetitive extragenic palindromic-PCR (rep-PCR) fingerprinting. The three smooth, rough and mucoid morphotypes presented different antibiotic resistance profiles and genotyping analysis showed that they belonged to distinct clones, indicating that at day 57 after the admission the patient was simultaneously colonized by three distinct P. aeruginosa isolates. On the other hand, the nine colony variants presented heterogeneous antibiotic resistance profiles and clustered together with the three parental isolates. The understanding of the link between genotype plasticity and antibiotic resistance may contribute to improving our knowledge of this life-threatening pathogen.
authors.affiliation: Unit of Microbiology, Bambino Gesù Children's Hospital, Healthcare and Research Institute, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Putignani
authors.forename: R
authors.initials: R
authors.lastname: Sessa
authors.forename: A
authors.initials: A
authors.lastname: Petrucca
authors.forename: C
authors.initials: C
authors.lastname: Manfredini
authors.forename: L
authors.initials: L
authors.lastname: Coltella
authors.forename: D
authors.initials: D
authors.lastname: Menichella
authors.forename: M
authors.initials: M
authors.lastname: Nicoletti
authors.forename: C
authors.initials: C
authors.lastname: Russo
authors.forename: P
authors.initials: P
authors.lastname: Cipriani
mesh_terms: D000368:Aged; D005838:Genotype; D006801:Humans; D007362:Intensive Care Units; D008168:Lung / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification