title: Effect of RGD-immobilized dual-pore poly(L-lactic acid) scaffolds on chondrocyte proliferation and extracellular matrix production.
abstract: Interactions between cell and polymer surface have great implications in tissue engineering. In this study, chondrocyte proliferation and matrix production were examined using porous poly(L-lactide) (PLLA) scaffolds that have different surface characteristics. PLLA scaffolds were prepared using a gas-foaming method, and subjected to surface modifications through plasma treatment and subsequent in situ grafting of hydrophilic acrylic acid (AA). To immobilize peptide ligands, the AA-grafted PLLA scaffolds (PLLA-PAA) were further reacted with either Gly-Arg-Asp-Gly (GRDG) or Gly-Arg-Gly-Asp (GRGD) to produce PLLA-PAA-GRDG or PLLA-PAA-GRGD scaffold, respectively. The average porosities of the scaffolds were more than 90%, and their pore sizes ranged from 200 approximately 300 to 10 approximately 50 microm for large and small pores, respectively. The concentrations of each bound component were 2.14 x 10(-4) mmol/cm(2) for AA, 1.87 nmol/g for GRDG, and 1.68 nmol/g for GRGD. When chondrocytes were seeded onto the different PLLA scaffolds, cell adhesion and proliferation were highly affected as the substrate types vary. The RGD-immobilized scaffolds resulted in higher cellularity and better accumulation of total glycosaminoglycan than the others. Histological staining of Safranin O showed that the deposited extracellular matrix was more intense and widely distributed in the PLLA-PAA-GRGD scaffold. The present data suggest that immobilization of RGD peptide on the AA-grafted PLLA scaffold can be an effective tool for chondrocyte attachment and proliferation, and that it may also be helpful to facilitate cartilaginous tissue formation.
authors.affiliation: Biomaterials Research Center, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Jung
authors.forename: Kwideok
authors.initials: K
authors.lastname: Park
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Kim
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Ki-Ok
authors.initials: KO
authors.lastname: Han
authors.forename: Dong Keun
authors.initials: DK
authors.lastname: Han
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D024043:Collagen Type II / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000179:Acrylates; D024043:Collagen Type II; D006025:Glycosaminoglycans; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C064597:glycyl-arginyl-glycyl-aspartic acid; C036658:acrylic acid