title: A high molecular weight polypeptide cross-reacting with the antibodies to the dynein heavy chain localizes to the subset of Golgi complex in higher plant cells.
abstract: Antibodies were produced against fragments of the microtubule-binding domain and the motor domain of the dynein heavy chain from Dictyostelium discoideum to probe whole cell extracts of root meristem cells of wheat Triticum aestivum. In plant extracts, these antibodies cross-reacted with a polypeptide of high molecular weight (>500kDa). The antibodies bound to protein A-Sepharose precipitated high molecular weight polypeptide from cell extracts. Immunofluorescence showed that the antibodies identified various aggregates inside cells, localized at the perinuclear area during interphase to early prophase, at the spindle periphery and polar area during mitosis, and in the interzonal region during phragmoplast development. Some aggregates were also co-labeled by markers for the Golgi apparatus. Thus, we found in higher plant cells a high molecular weight antigen cross-reacting with the antibodies to motor and microtubule-binding domains of dynein heavy chains. This antigen is associated with aggregates distributed in the cytoplasm in cell cycle-dependent manner. A subset of these aggregates belongs to the Golgi complex.
authors.affiliation: Department of Molecular Biology, Moscow State University, Russia.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Shanina
authors.forename: Elena M
authors.initials: EM
authors.lastname: Lazareva
authors.forename: Ekaterina Y
authors.initials: EY
authors.lastname: Skorova
authors.forename: Yuri S
authors.initials: YS
authors.lastname: Chentsov
authors.forename: Elena A
authors.initials: EA
authors.lastname: Smirnova
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D003429:Cross Reactions; D004023:Dictyostelium / Q000276:immunology; D004398:Dyneins / Q000276:immunology*; D006056:Golgi Apparatus / Q000737:chemistry* / Q000276:immunology; D007399:Interphase; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology*; D011418:Prophase; D014908:Triticum / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D010455:Peptides; D010940:Plant Proteins; D004398:Dyneins