Properties of astrocytes cultured from GFAP over-expressing and GFAP mutant mice.
Alexander disease is a fatal leukoencephalopathy caused by dominantly-acting coding mutations in GFAP. Previous work has also implicated elevations in absolute levels of GFAP as central to the pathogenesis of the disease. However, identification of the critical astrocyte functions that are compromised by mis-expression of GFAP has not yet been possible. To provide new tools for investigating the nature of astrocyte dysfunction in Alexander disease, we have established primary astrocyte cultures from two mouse models of Alexander disease, a transgenic that over-expresses wild type human GFAP, and a knock-in at the endogenous mouse locus that mimics a common Alexander disease mutation. We find that mutant GFAP, as well as excess wild type GFAP, promotes formation of cytoplasmic inclusions, disrupts the cytoskeleton, decreases cell proliferation, increases cell death, reduces proteasomal function, and compromises astrocyte resistance to stress.
Cho
Woosung
W
Waisman Center, Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI 53705, USA.
Messing
Albee
A
D038261:Alexander Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D016877:Oxidants; D038203:alpha-Crystallin B Chain; D006861:Hydrogen Peroxide; D020169:Caspases; D046988:Proteasome Endopeptidase Complex