Synthesis of Ag-Pt alloy nanoparticles in aqueous bovine serum albumin foam and their cytocompatibility against human gingival fibroblasts.
Foams of bovine serum albumin (BSA) have been utilized for synthesizing in situ protein capped Ag-Pt alloy nanoparticles. The in vitro cytotoxicity and the rate of proliferation of human gingival fibroblasts (HGFs) in presence of the above synthesized alloy nanoparticles is investigated. Expression profile of protein involved in detoxification, i.e. metallotheonein (MT) were assayed by ELISA and expression of mRNA transcripts by reverse transcription polymerase chain reaction (RT-PCR). Cytotoxicity results suggested that protein capped nano-alloys might be promising candidates for implants and prosthetic material. RT-PCR and ELISA confirmed the expression of MT, in cells treated with the alloy nanoparticles. Morphology variation studied by SEM also confirms that cells treated with alloy nanoparticles present an intact morphology.
Singh
A V
AV
Materials Chemistry Division, National Chemical Laboratory, Pune, India.
Patil
R
R
Kasture
M B
MB
Gade
W N
WN
Prasad
B L V
BL
D000293:Adolescent; D000328:Adult; D000497:Alloys / Q000138:chemical synthesis* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles; D008668:Metallothionein / Q000096:biosynthesis; D010984:Platinum / Q000737:chemistry; D019736:Prostheses and Implants; D012710:Serum Albumin, Bovine; D012834:Silver / Q000737:chemistry
D000497:Alloys; D001672:Biocompatible Materials; D012710:Serum Albumin, Bovine; D012834:Silver; D010984:Platinum; D008668:Metallothionein