title: Lysine restriction and realimentation affected growth, blood  profiles and expression of genes related to protein and fat  metabolism in weaned pigs.
abstract: To investigate the effects of lysine restriction and subsequent realimentation on growth performance, blood profiles and gene expression of leptin and myostatin, 128 weaned pigs [initial body weight (BW) 6.96 ± 1.07 kg, 26 ± 2 days of age] were randomly allotted to four treatments. The starter diets during the first 2 weeks (P1) contained 100%, 80%, 70% or 60% of recommended lysine levels (National Research Council, 1998). Then, common grower 1 and 2 diets were offered for 2 weeks (P2 and P3) each. During P1, average daily gain (ADG) was linearly reduced (p < 0.05) with the increasing levels of lysine restriction. Growth rate was greater in pigs previously fed lysine-restricted diets than well-fed pigs although it did not reach a significant level during realimentation. However, the final BW and overall ADG were the lowest (p < 0.05) and F/G was poor in pigs fed 60% lysine diet. Relative visceral organ weights and composition of skeletal muscle were similar (p > 0.05) among the treatment. Blood triglyceride and glucose levels were increased (p < 0.05) during P1, while blood urine nitrogen, total protein and albumin levels were decreased (p < 0.05) during P2 with the reduction in dietary lysine levels. The abundance of myostatin mRNA in skeletal muscle and leptin mRNA in subcutaneous adipose tissue were lower (p < 0.05) in lysine-restricted pigs than in pigs fed non-restricted diets. In conclusion, 80% and 70% lysine restriction of starter diets resulted in inferior growth and compensatory growth effect was noted during realimentation, while 60% lysine restriction had a negative influence on growth performance. Moreover, the changes in myostatin and leptin mRNA abundance caused by nutritional manipulations may be involved in the regulation of protein and fat deposition in young pigs.
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon, Korea.
authors.forename: Y X
authors.initials: YX
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: Z
authors.initials: Z
authors.lastname: Jin
authors.forename: S Y
authors.initials: SY
authors.lastname: Yoon
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: M H
authors.initials: MH
authors.lastname: Wang
authors.forename: X S
authors.initials: XS
authors.lastname: Piao
authors.forename: B W
authors.initials: BW
authors.lastname: Kim
authors.forename: B J
authors.initials: BJ
authors.lastname: Chae
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary*; D004041:Dietary Fats / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D008239:Lysine / Q000008:administration & dosage* / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D013552:Swine / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D011506:Proteins; D008239:Lysine