title: Carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a Canadian mill.
abstract: Forty-one bacterial strains isolated from the headbox water of two machines in a Canadian paper mill were associated with the genera Asticcacaulis, Acidovorax, Bacillus, Exiguobacterium, Hydrogenophaga, Pseudomonas, Pseudoxanthomonas, Staphylococcus, Stenotrophomonas based on the sequence of their 16S rRNA genes. The metabolic profile of these strains were determined using Biolog EcoPlate, and the bacteria were divided into four metabolic groups. Metabolic profiles of the bacterial communities colonizing the headbox water of two paper machines was also determined weekly over a 1 year period. The only compound that was not reduced by the bacterial community was 2-hydroxybenzoic acid. Utilization frequency of the other carbon sources in the Biolog EcoPlate ranged from 3 to 100%. The metabolic profiles of the bacterial community did not vary considerably between the two paper machines. However, the metabolic profile varied among the sampling dates.
authors.affiliation: Centre d'Etude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada.
authors.forename: Johnny
authors.initials: J
authors.lastname: Kashama
authors.forename: Véronique
authors.initials: V
authors.lastname: Prince
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Simao-Beaunoir
authors.forename: Carole
authors.initials: C
authors.lastname: Beaulieu
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D002170:Canada; D002244:Carbon / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D007221:Industry / Q000295:instrumentation; D010209:Paper; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002244:Carbon