title: Determination of relative assay response factors for toxic chlorinated and brominated dioxins/furans using an enzyme immunoassay (EIA) and a chemically-activated luciferase gene expression cell bioassay (CALUX).
abstract: Determination of toxic activity requires knowledge of both the concentration and toxicity to evaluate the risk for adverse human health and environmental effects. A chemically-activated luciferase gene expression cell bioassay system (CALUX) and an antibody-based method enzyme immunoassay (EIA) were used to detect the dioxin-like response of several polybrominated, polychlorinated, and polybrominated/chlorinated dibenzo-p-dioxins/furans (PBDDs/Fs, PCDDs/Fs, and PBCDDs/Fs, respectively). It has been suggested that the biological activity of the brominated and mixed bromo/chloro compounds is similar to their chlorinated analogues (measured by binding to the Ah receptor). PBDD/F, PCDD/F, and PBCDD/F laboratory standards exhibited biological activity ranging over three orders of magnitude. The highest relative potency (REP) values from CALUX analysis, when compared to 2,3,7,8-TCDD, were 2,3,7,8-TBDD at 0.99 (+/-0.07), 1,2,3,7,8-PeCDD at 0.69, and 2-Br-3,7,8-TriCDD at 0.72 (+/-0.02). Cross-reactivities were calculated using EIA for several PBDDs/Fs and PBCDDs. The highest percent cross-reactivity was found for 2,3,7,8-TBDD at 138 (+/-34%), and 2,3,7-TriBDD at 84 (+/-36%).
authors.affiliation: U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 109 T.W. Alexander Drive, Research Triangle Park, NC 27711, USA.
authors.forename: Fatin
authors.initials: F
authors.lastname: Samara
authors.forename: Brian K
authors.initials: BK
authors.lastname: Gullett
authors.forename: Robert O
authors.initials: RO
authors.lastname: Harrison
authors.forename: Andrew
authors.initials: A
authors.lastname: Chu
authors.forename: George C
authors.initials: GC
authors.lastname: Clark
mesh_terms: D001681:Biological Assay / Q000379:methods; D003429:Cross Reactions; D004147:Dioxins / Q000032:analysis* / Q000633:toxicity*; D054750:Ecotoxicology / Q000379:methods*; D005663:Furans / Q000032:analysis* / Q000633:toxicity*; D015870:Gene Expression; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000032:analysis / Q000633:toxicity; D006843:Hydrocarbons, Chlorinated / Q000032:analysis / Q000633:toxicity; D007124:Immunoenzyme Techniques / Q000379:methods; D008156:Luciferases / Q000378:metabolism
chemical_list: D004147:Dioxins; D005663:Furans; D006842:Hydrocarbons, Brominated; D006843:Hydrocarbons, Chlorinated; D008156:Luciferases