Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion.
OBJECTIVE
Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesized that within this time, inflammatory cytokines might accumulate.


METHODS
Serial plasma samples were collected 4, 10 and 20 h after donation and cytokine concentrations were measured.


RESULTS
Interleukin-8 increased > 20-fold and soluble CD40 ligand > sixfold during the observation time, less pronounced changes for several other mediators were also observed.


CONCLUSIONS
Leucocyte depletion within 10 h of blood donation will reduce the concentrations of pyrogenic mediators.
Chudziak
D
D
German Red Cross Blood Centre and Institute for Transfusion Medicine and Immunohaematology of the Johann-Wolfgang-Goethe University, Frankfurt, Germany.
Sireis
W
W
Pfeiffer
H-U
HU
Henschler
R
R
Seifried
E
E
Bönig
H
H
D001782:Blood Donors; D001793:Blood Preservation; D005334:Fever / Q000209:etiology; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D047589:Leukocyte Reduction Procedures; D012995:Solubility; D013997:Time Factors; D065227:Transfusion Reaction
D018836:Inflammation Mediators