Glyceraldehyde is present in rat lens and its level is increased in diabetes mellitus.
OBJECTIVE
OP-lysine, a glycation product of lysine residues of proteins, has been reported to be formed with glyceraldehyde and glycolaldehyde as precursors in the lens, and has been suggested to play a role in senile cataracts. However, there has been no reliable information regarding the content of glyceraldehyde in tissues. This study determined the glyceraldehyde levels in the lenses of normal and diabetic rats.


METHODS
Glyceraldehyde was derivatized to a fluorescent compound, and the compound was then quantified by high-performance liquid chromatography.


RESULTS
The lens glyceraldehyde levels in normal and diabetic rats were 0.75 +/- 0.06 and 1.26 +/- 0.21 nmol/g wet weight (means +/- standard deviations of 6 animals, p < 0.01), respectively. Isolated rat lenses accumulated a higher level of glyceraldehyde when cultured for 6 days in 25.5 mM glucose than when cultured in 5.5 mM glucose.


CONCLUSIONS
Glyceraldehyde was found to be present in the lens and was increased in diabetes mellitus. OP-lysine is thus likely to be a potential risk factor for senile and diabetic cataracts.
Miwa
I
I
Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan. miwaichi@ccmfs.meijo-u.ac.jp
Chen
A-S
AS
Taguchi
T
T
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D001572:Benzofurans / Q000494:pharmacology; D001786:Blood Glucose / Q000032:analysis; D002386:Cataract / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D005985:Glyceraldehyde / Q000378:metabolism*; D006827:Hydantoins / Q000494:pharmacology; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D011726:Pyridinium Compounds / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
C484321:2-amino-6-(3-oxidopyridinium-1-yl)hexanoic acid; D001572:Benzofurans; D001786:Blood Glucose; D006827:Hydantoins; D011726:Pyridinium Compounds; C068408:M 16287; D005985:Glyceraldehyde; D000449:Aldehyde Reductase; D008239:Lysine