Proteome analysis for 3T3-L1 adipocyte differentiation.
Adipose tissue is an important endocrine organ involved in the control of whole body energy homeostasis and insulin sensitivity. Considering the increased incidence of obesity and obesity-related disorders, including diabetes, it is important to understand thoroughly the process of adipocyte differentiation and its control. Therefore, we performed a differential proteome mapping strategy using two-dimensional gel electrophoresis combined with peptide mass fingerprinting to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3-L1 pre-adipocytes in response to an adipogenic cocktail. In the current study, we identified 46 differentially expressed proteins, 6 of which have not been addressed previously in 3T3-L1 cell differentiation. Notably, we found that phosphoribosyl pyrophosphate synthetase (PRPS), a regulator of cell proliferation, was preferentially expressed in pre-adipocytes than in fully differentiated adipocytes. In conclusion, our results provide valuable information for further understanding of the adipogenic process.
Atiar Rahman
Md
M
Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Korea.
Kumar
Suresh G
SG
Lee
Sung Hak
SH
Hwang
Hee Sun
HS
Kim
Hyun Ah
HA
Yun
Jong Won
JW
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D051379:Mice; D010449:Peptide Mapping; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D015203:Reproducibility of Results; D012268:Ribose-Phosphate Pyrophosphokinase / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011506:Proteins; D020543:Proteome; D012268:Ribose-Phosphate Pyrophosphokinase