Malignancy grade-dependent expression of K+-channel subtypes in human prostate cancer.
K(+) channels are key molecules in the progression of several cancer types and considered to be potential targets for cancer therapy. We examined the gene expressions of voltage-gated (K(v)), Ca(2+)-activated (K(Ca)), and two-pore domain (K(2P)) K(+)-channel subtypes in needle-biopsy samples of human prostate cancer (PCa) by real-time PCR and compared them with those in PCa epithelial cell lines. The expression of K(v)1.3, K(Ca)1.1, K(Ca)3.1, and K(2P)1 markedly increased in the PCa group with Gleason score of 5 - 6 (GS5-6) but significantly decreased in the GS8-9 group. This malignancy grade-dependent K(+)-channel expression pattern may provide a convenient marker to understand PCa progression level.
Ohya
Susumu
S
Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Kimura
Kazunori
K
Niwa
Satomi
S
Ohno
Akitoshi
A
Kojima
Yoshiyuki
Y
Sasaki
Shoichi
S
Kohri
Kenjiro
K
Imaizumi
Yuji
Y
D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D015221:Potassium Channels / Q000235:genetics*; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D020033:Protein Isoforms / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D015221:Potassium Channels; D020033:Protein Isoforms; D017430:Prostate-Specific Antigen