CMTM5-v1 induces apoptosis in cervical carcinoma cells.
CMTM5 (CKLF-like MARVEL transmembrane domain-containing member 5) exhibits tumor inhibition activity with frequent epigenetic inactivation in various tumor cell lines including cervical carcinoma (CC) cells. In this paper, we examined the function of CMTM5-v1 (the primary RNA splicing form) in both HeLa and SiHa cells. Overexpression of CMTM5-v1 in both cells can induce apoptosis, but the effects are more obvious in SiHa than that in HeLa. In SiHa cells, restoration of CMTM5-v1 caused disruption of mitochondrial transmembrane potential, release of cytochrome c, activation of caspase3 and cleavage of PARP. General caspase inhibitor almost prevented apoptosis of SiHa cells, suggesting that CMTM5-v1 induces apoptosis mainly through caspase-dependent pathway. These findings verify that CMTM5-v1 inhibits the growth of CC cell lines via inducing apoptosis.
Shao
Luning
L
Peking University Center for Human Disease Genomics, Department of Immunology, Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China.
Guo
Xiaohuan
X
Plate
Markus
M
Li
Ting
T
Wang
Yu
Y
Ma
Dalong
D
Han
Wenling
W
D017209:Apoptosis; D002277:Carcinoma / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D018925:Chemokines / Q000235:genetics / Q000502:physiology*; D045304:Cytochromes c / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D062792:MARVEL Domain-Containing Proteins; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
C525020:CMTM5 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D025521:Tumor Suppressor Proteins; D045304:Cytochromes c; D053148:Caspase 3