[Generation of human Fab antibody against platelet membrane glycoprotein IIb/IIIa and its effect on platelet aggregation].
OBJECTIVE
To generation of human Fab fragment against GP GPIIb/IIIa from phage display library and to study its effect on platelet aggregation.


METHODS
An antibody phage display library was constructed from spleen cells from a donor with idiopathic thrombocytopenic purpura(ITP) whose anti-GPIIb/IIIa antibody was positive. High-affinity human mAbs was selected by panning against GPIIb/IIIa expressed on CHO123 cells. The binding specificity of phage antibody to GPIIb/IIIa was detected by ELISA and Western blot. And the effect of antibody on platelet aggregation was analyzed.


RESULTS
After three rounds of panning with CHO123, phage antibodies against GPIIb/IIIa were enriched specifically. 2 positive phage antibodies with high specific for GP IIb/IIIa were verified, and the purified antibody can inhibited ADP induced platelet aggregation.


CONCLUSIONS
Human antibodies against GPIIb/IIIa are obtained from phage display library.
Dong
Ning-Zheng
NZ
The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Ministry of Health, Suzhou, China. ningzheng_dong@yahoo.com.cn
Cui
Yu-Jie
YJ
Ruan
Chang-Geng
CG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019151:Peptide Library; D010974:Platelet Aggregation / Q000187:drug effects*; D040201:Platelet Membrane Glycoprotein IIb / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D039661:Integrin beta3; D019151:Peptide Library; D040201:Platelet Membrane Glycoprotein IIb