title: Lack of association between the TNF-alpha -308 (G/A) genetic polymorphism and periodontal disease in Brazilians.
abstract: This study evaluated the frequency of the tumor necrosis factor-alpha (TNF-alpha) -308 G/A polymorphism in Brazilians with periodontal health (PH = 51), chronic periodontitis (CP = 74) and generalized aggressive periodontitis (GAgP = 38). Human DNA was obtained from mouthwash samples and TNF-alpha genotyping was performed by PCR and RFLP analyses. Differences in clinical and genetic parameters among groups were sought by Kruskal-Wallis, chi(2) and Fisher's exact tests. The allele -308G was detected in 91.7%, whereas the allele -308A was found in 35.4% of all subjects. No significant differences were observed in the frequency of these alleles (chi(2) = 2.610, p > 0.05) and the genotypes G/G, G/A, and A/A (chi(2) = 2.547, p = 0.636) among groups. The data suggest that the TNF-alpha -308 G/A polymorphism is not associated with periodontitis in this Brazilian population.
authors.affiliation: School of Dentistry, Federal University of Rio de Janeiro, Brazil. nataschagiovannetti@yahoo.com.br
authors.forename: Natascha Giovannetti de
authors.initials: NG
authors.lastname: Menezes
authors.forename: Ana Paula Vieira
authors.initials: AP
authors.lastname: Colombo
mesh_terms: D000293:Adolescent; D010520:Aggressive Periodontitis / Q000235:genetics; D000483:Alleles; D001938:Brazil; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D009909:Oral Health; D010510:Periodontal Diseases / Q000175:diagnosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D005819:Genetic Markers; D014409:Tumor Necrosis Factor-alpha