Dynamin 2 cooperates with amphiphysin 1 in phagocytosis in sertoli cells.
Testicular Sertoli cells highly express dynamin 2 and amphiphysin 1. Here we demonstrate that dynamin 2 is implicated in phosphatidylserine (PS)-dependent phagocytosis in Sertoli cells. Immunofluorescence and dual-live imaging revealed that dynamin 2 and amphiphysin 1 accumulate simultaneously at ruffles. These proteins are specifically bound in vitro. Over-expression of dominant negative dynamin 2 (K44A) inhibits liposome-uptake and leads to the mis-localization of amphiphysin 1. Thus, the cooperative function of dynamin 2 and amphiphysin 1 in PS-dependent phagocytosis is strongly suggested.
Nakanishi
Akira
A
Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Abe
Tadashi
T
Watanabe
Masami
M
Takei
Kohji
K
Yamada
Hiroshi
H
D000818:Animals; D002478:Cells, Cultured; D034285:Dynamin II / Q000235:genetics / Q000378:metabolism*; D034286:Dynamin III / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology*; D010718:Phosphatidylserines / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D014162:Transfection
D009419:Nerve Tissue Proteins; D010718:Phosphatidylserines; D049452:Green Fluorescent Proteins; C075637:amphiphysin; D034285:Dynamin II; D034286:Dynamin III