[Physiological and biochemical properties of the bacterium Erwinia rhapontici, a producer of isomaltulose synthase].
Optimum conditions for the biosynthesis of isomaltulose synthase by submerged cultures of the bacterium Erwinia rhapontici, grown in the presence of 10% sucrose, have been determined (temperature of culturing, 30 degrees C; initial pH level, 7.5; duration of culturing, 54 h). The electrophoretically homogeneous preparation of the enzyme, thus obtained, had a specific activity of 210 U/mg protein. Optimum function of the enzyme was observed at 30 degrees C and pH 6.0. Isomaltulose synthase exhibited maximum stability at 20-30 degrees C and pH 6.0-7.0. The catalytic activity of the enzyme amounted to 3300 U/cm3, which is 40 to 50 times higher that the values reported for strains studied previously.
Korneeva
O S
OS
Bozhko
O Iu
OIu
Mangueva
Z M
ZM
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D004795:Enzyme Stability; D004885:Erwinia / Q000201:enzymology* / Q000254:growth & development; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019751:Intramolecular Transferases / Q000737:chemistry* / Q000302:isolation & purification; D013379:Substrate Specificity / Q000502:physiology
D001426:Bacterial Proteins; D019751:Intramolecular Transferases; C042002:isomaltulose synthase