Optimization of separation and online sample concentration of N,N-dimethyltryptamine and related compounds using MEKC.
The optimal separation conditions and online sample concentration for N,N-dimethyltryptamine (DMT) and related compounds, including alpha-methyltryptamine (AMT), 5-methoxy-AMT (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DiPT), 5-methoxy-DMT (5-MeO-DMT), and 5-methoxy-N,N-DiPT (5-MeO-DiPT), using micellar EKC (MEKC) with UV-absorbance detection are described. The LODs (S/N = 3) for MEKC ranged from 1.0 1.8 microg/mL. Use of online sample concentration methods, including sweeping-MEKC and cation-selective exhaustive injection-sweep-MEKC (CSEI-sweep-MEKC) improved the LODs to 2.2 8.0 ng/mL and 1.3 2.7 ng/mL, respectively. In addition, the order of migration of the nine tryptamines was investigated. A urine sample, obtained by spiking urine collected from a human volunteer with DMT, was also successfully examined.
Wang
Man-Juing
MJ
Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.
Tsai
Chih-Hsin
CH
Hsu
Wei-Ya
WY
Liu
Ju-Tsung
JT
Lin
Cheng-Huang
CH
D020374:Chromatography, Micellar Electrokinetic Capillary; D015394:Molecular Structure; D004130:N,N-Dimethyltryptamine / Q000737:chemistry* / Q000302:isolation & purification*; D009862:Online Systems; D013057:Spectrum Analysis
D004130:N,N-Dimethyltryptamine