title: Alternative splice variants of rat 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase-4 mRNA.
abstract: Expression of 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-4 (PFKFB-4) mRNA was studied in different rat organs. Several new unique alternative splice variants of PFKFB-4 mRNA were identified. They have deletions or inserts in fructose-2,6-bisphosphatase region as well as different length and amino acid sequence of C-terminal part. However, 6-phosphofructo-2-kinase catalytic domains were identical in all variants. Moreover, the expression of different alternative splice variants of PFKFB-4 mRNA has shown tissue specificity. Expression of both alternative splice variants of PFKFB-4 mRNA significantly changed in rats treated by methyl tretbutyl ether, ecologically dangerous chemical compound. Results of this investigation indicate a possible role of PFKFB-4 splice isoforms in cell-specific regulation of glycolysis and demonstrate the sensitivity of the regulation of alternative splicing to the action of toxic chemical compounds, in particular methyl tertbutyl ether.
authors.affiliation: Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv. ominchenko@yahoo.com
authors.forename: D O
authors.initials: DO
authors.lastname: Minchenko
authors.forename: V G
authors.initials: VG
authors.lastname: Mykhalchenko
authors.forename: K
authors.initials: K
authors.lastname: Tsuchihara
authors.forename: S
authors.initials: S
authors.lastname: Kanehara
authors.forename: O P
authors.initials: OP
authors.lastname: Yavorovsky
authors.forename: I V
authors.initials: IV
authors.lastname: Zavgorodny
authors.forename: Yu O
authors.initials: YO
authors.lastname: Paustovsky
authors.forename: S V
authors.initials: SV
authors.lastname: Komisarenko
authors.forename: H
authors.initials: H
authors.lastname: Esumi
authors.forename: O H
authors.initials: OH
authors.lastname: Minchenko
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D004785:Environmental Pollutants / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008738:Methyl Ethers / Q000633:toxicity; D008969:Molecular Sequence Data; D009928:Organ Specificity; D025481:Phosphofructokinase-2 / Q000235:genetics*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004785:Environmental Pollutants; D008738:Methyl Ethers; D020033:Protein Isoforms; D012333:RNA, Messenger; C043243:methyl tert-butyl ether; D025481:Phosphofructokinase-2