Whole-body section fluorescence imaging--a novel method for tissue distribution studies of fluorescent substances.
The present study demonstrates the usefulness of whole body section fluorescence imaging, a novel technique used in optical imaging drug discovery. This method is in principle an analog of whole body autoradiography, except that fluorescence is measured instead of radioactivity. The method was shown to have a linear concentration-response relationship over a 1000-fold concentration range. Densitometric image analysis allowed semiquantitative studies of drug disposition and selective tumor retention of an optical imaging drug candidate.
Moestue
Siver
S
Research and Development, GE Healthcare, Nycoveien 2, N-0401 Oslo, Norway. siver.a.moestue@ntnu.no
Nunez
Paula
P
Healey
Andrew
A
Bjerke
Roger
R
Indrevoll
Bård
B
Skotland
Tore
T
Hustvedt
Svein Olaf
SO
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014018:Tissue Distribution; D014915:Whole-Body Counting / Q000379:methods*