Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens.
The genus Listeria is composed of six species of which Listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. The results indicated the presence of L. monocytogenesin the clinical sample studied. Moreover, the isolate was identified as the 4b serovar that was characterized by the presence of a unique 691 bp band after analysis using the Multiplex-PCR technique. The results of repeated Multiplex-PCR and sequencing have indicated that the L. monocytogenes isolate was an atypical 4b serovar, which is the first time this finding has been reported.
Vasconcelos
Rafaela Moledo de
RM
Programa de Pós-Graduação em Vigilância Sanitária, Instituto Nacional de Controle e Qualidade em Saúde-FIOCRUZ, Rio de Janeiro, RJ, Brasil. rafamoledo@gmail.com
Almeida
Antonio Eugenio Castro Cardoso de
AE
Hofer
Ernesto
E
Silva
Nicéa Magaly Matias da
NM
Marin
Victor Augustus
VA
D015373:Bacterial Typing Techniques; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008088:Listeriosis / Q000134:cerebrospinal fluid*; D016133:Polymerase Chain Reaction / Q000379:methods*