Fluorometric determination of sulfite in wine by N-(9-acridinyl)maleimide.
A highly sensitive fluorometric method for determining sulfite in wine is reported. N-(9-Aciridinyl)maleimide (NAM) reacted with the sulfite in wine quantitatively and gave strong fluorescent derivatives. The determinations were conducted by batch and high-performance liquid chromatographic methods. Sulfite contents in wine by NAM methods and the conventional methods showed good accordance. The sample size could be reduced to less than 1/10,000 by the NAM methods.
Akasaka
K
K
Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981, Japan.
Matsuda
H
H
Ohrui
H
H
Meguro
H
H
Suzuki
T
T
D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D005504:Food Analysis / Q000379:methods; D007202:Indicators and Reagents; D008301:Maleimides; D013447:Sulfites / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D007202:Indicators and Reagents; D008301:Maleimides; D013447:Sulfites; C080490:N-(9-acridinyl)maleimide