title: Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays.
abstract: Sixty-one avian strains of Pasteurella multocida were characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified as P. multocida subsp. multocida and 19 strains (31%) as P. multocida subsp. septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other capsular types were not identified. From the 61 isolates 24 different fingerprint patterns were generated by ERIC-PCR assay. Based on cluster analysis the strains could be grouped into four larger and four mini-clusters that showed considerable correlation with the geographical origin and the host species. The results indicate that ERIC-PCR may be a suitable technique for studying the host adaptation of P. multocida and the epidemiology of fowl cholera.
authors.affiliation: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, H-1581 Budapest, P.O. Box 18, Hungary.
authors.forename: Boglárka
authors.initials: B
authors.lastname: Sellyei
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Varga
authors.forename: Eva
authors.initials: E
authors.lastname: Ivanics
authors.forename: Tibor
authors.initials: T
authors.lastname: Magyar
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000382:microbiology; D001717:Birds; D006814:Hungary / Q000453:epidemiology; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013045:Species Specificity