Delivery of sodium borocaptate to glioma cells using immunoliposome conjugated with anti-EGFR antibodies by ZZ-His.
Nanoparticles are effective of delivering cargo into cells. Here, sodium borocaptate (BSH) was encapsulated in liposomes composed of nickel lipid, and anti-epidermal growth factor receptor (EGFR) antibodies were conjugated to the liposomes using the antibody affinity motif of protein A (ZZ) as an adaptor (immunoliposomes). The immunoliposomes were used to deliver BSH into EGFR-overexpressing glioma cells. Immunohistochemical analysis using an anti-BSH monoclonal antibody revealed that BSH was delivered effectively into the cells but not into EGFR-deficient glioma or primary astrocytes. In an animal model of brain tumors, both the liposomes and the BSH were only observed in the tumor. Moreover, the efficiency of (10)B's delivery into glioma cells was confirmed by inductively coupled plasma-atomic emission spectrometry (ICP-AES) both in vitro and in vivo. The results suggest that this system utilizing immunoliposomes provides an effective means of delivering (10)B into glioma cells in boron neutron capture therapy (BNCT).
Feng
Bin
B
Department of Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
Tomizawa
Kazuhito
K
Michiue
Hiroyuki
H
Miyatake
Shin-ichi
S
Han
Xiao-Jian
XJ
Fujimura
Atsushi
A
Seno
Masaharu
M
Kirihata
Mitsunori
M
Matsui
Hideki
H
D020816:Amino Acid Motifs; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001894:Borohydrides / Q000008:administration & dosage* / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D066246:ErbB Receptors / Q000276:immunology*; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008081:Liposomes; D051379:Mice; D008819:Mice, Nude; D051381:Rats; D013054:Spectrophotometry, Atomic; D013205:Staphylococcal Protein A / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000008:administration & dosage* / Q000627:therapeutic use*; D013997:Time Factors; D023041:Xenograft Model Antitumor Assays
D000911:Antibodies, Monoclonal; D001894:Borohydrides; D008081:Liposomes; D013205:Staphylococcal Protein A; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate; D066246:ErbB Receptors