Bending rigidities of cell surface molecules P-selectin and PSGL-1.
P-selectin is a cell adhesion molecule expressed on activated endothelial cells and platelets. P-selectin glycoprotein ligand 1 (PSGL-1) is a mucin expressed on leukocytes. The interaction of P-selectin and PSGL-1 mediates leukocyte tethering to and rolling on the vascular surface, which are initiating events in inflammatory and thrombotic processes. In the hemodynamic environment of the circulation, P-selectin and PSGL-1 are subject to a wide range of forces, which can cause deformation. For P-selectin/PSGL-1 interaction to be physically possible, these molecules may need to project above much of the glycocalyx layers of the respective cell surfaces, suggesting that they are either longer than the thickness of glycocalyx or better able to support compression than the glycocalyx. As such, the mechanical properties of these molecules and their functional implications merit investigation. Here we report determination of the bending rigidities of P-selectin and PSGL-1 by analyzing their thermally excited curvature fluctuations, whose values are of the order of magnitude of 100pNnm(2).
Fang
Ying
Y
Institute of Biomechanics and School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Wu
Jianhua
J
McEver
Rodger P
RP
Zhu
Cheng
C
D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism; D003198:Computer Simulation; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D019007:P-Selectin / Q000378:metabolism / Q000648:ultrastructure*
D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein