Expression of CD320 in human B cells in addition to follicular dendritic cells.
CD320 has been recently discovered and reported as a follicular dendritic cell (FDC) protein. Although CD320 is known to enhance proliferation of germinal center (GC) B cells, little other information is available. In this study, we investigated its cellular distribution in the GC. Confocal microscopy of human tonsil sections revealed co-localization of CD320 with CD19 and CD38 but not with CD3 indicating that GC B cells expressed CD320 in addition to FDC. In purified GC B cells, CD320 expression was inhibited in the nucleus, membrane and cytoplasm. Reverse transcriptase-polymerase chain reaction confirmed CD320 mRNA expression in B cells. These finding indicate that CD320 is expressed in B cells in addition to FDC, and that its GC activity may be more complicated than previously thought.
Cho
Whajung
W
Department of Microbiology and Immunology, Kangwon National University College of Medicine, Chuncheon, Korea.
Choi
Jinsuk
J
Park
Chan-Hum
CH
Yoon
Sun-Ok
SO
Jeoung
Doo-Il
DI
Kim
Young-Myeong
YM
Choe
Jongseon
J
D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D018941:Antigens, CD19 / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D017252:CD3 Complex / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D020566:Dendritic Cells, Follicular / Q000378:metabolism*; D015870:Gene Expression; D018858:Germinal Center / Q000166:cytology / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000378:metabolism; D018613:Microscopy, Confocal; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D015703:Antigens, CD; D018941:Antigens, CD19; D017252:CD3 Complex; C538894:CD320 protein, human; D017931:DNA Primers; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1