title: Expression of cardiac myosin light chain 2 during embryonic heart development in medaka fish, Oryzias latipes, and phylogenetic relationship with other myosin light chains.
abstract: Cardiac myosin light chain 2 (MLC-2) plays a key role in heart development, contraction, and embryo and adult heart maintenance. In some animals, defects in the function of cardiac MLC-2 cause hypertrophic cardiomyopathy. To illuminate the functions of cardiac MLC-2 in embryonic heart formation and contraction, and into the evolution of MLC-2, we characterized the expression and requirement for medaka cardiac MLC-2 gene in the developing heart. Medaka cardiac MLC-2 cDNA (mcmlc2) was isolated and its gene expression pattern was determined. The mcmlc2 was found to be expressed in the bilateral cardiac mesoderm, the formed heart tube, and in both the differentiated ventricle and atrium. Knockdown of mcmlc2 function caused severe cardiac disorders, including edema in the atrium and sinus venosus. Using phylogenetic analysis, we found that physiological variations in the MLC-2 molecules evolved due to amino acid changes in the Ca(2+) binding domain during molecular evolution. Our findings concerning the function and expression of mcmlc2 are nearly identical with those of other MLC-2 genes, and our phylogenetic analysis suggests that during evolution, the variations in physiological function within the MLC-2 gene family have arisen from a change in the amino acids in the Ca(2+) binding domain in the MLC-2 molecule.
authors.affiliation: Department of Animal Science, University of California, Davis, Davis, California 95616, USA.
authors.forename: Eriko
authors.initials: E
authors.lastname: Shimada
authors.forename: Masato
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Kenji
authors.initials: K
authors.lastname: Murata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D024722:Cardiac Myosins / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009154:Mutation; D009206:Myocardium / Q000378:metabolism; D018994:Myosin Light Chains / Q000145:classification / Q000235:genetics*; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D018994:Myosin Light Chains; C107184:myosin light chain 2; D024722:Cardiac Myosins