A laminin-rich basement membrane matrix influences estrogen receptor beta expression and morphology of MDA-MB-231 breast cancer cells.
The expression of the estrogen receptor beta (ERbeta) has been shown to play an important role in breast cancer. There is emerging hope that ERbeta and its isoforms will be used as prognostic markers or as therapeutic targets in the clinical management of breast cancer. Many studies indicate that ERbeta is down regulated during carcinogenesis. However, it is still unknown which signals can regulate ERbeta expression. Basement membrane (BM) components have been shown to influence the expression levels of ERalpha and progesterone receptor. Therefore, we hypothesized that cell-matrix interactions can also affect the expression of ERbeta and its isoforms. To test this we performed Matrigel assays using an ERalpha negative breast cancer cell line. MDA-MB-231 cells were plated on Matrigel, a reconstituted laminin-rich BM matrix, or on uncoated plastic culture plates. To investigate the effects of specific BM components we also cultured the cells on gels of purified collagen type IV and laminin-111. ERbeta expression levels were investigated after 24, 48 and 72 h by RT-PCRs which allow to distinguish between different ERbeta isoforms. MDA-MB-231 cells cultured on tissue culture plastic showed increased levels of ERbeta1 mRNA after 48 h. However, in cells cultured on Matrigel signals for ERbeta1 expression stayed at very low, nearly undetectable levels. Laminin-111 was identified to be the protein that represses ERbeta1 expression at the mRNA stage. Collagen type IV showed no effect on ERbeta expression. We further observed that MDA-MB-231 cells on Matrigel organize into cell aggregates which are connected in web-like structures that appear similar to lactiferous ducts. These data suggest that interactions of breast cancer cells with the BM protein laminin-111 suppress the expression of ERbeta1 at the mRNA level. A laminin-111-rich microenvironment seems to keep ERbeta1 at very low levels in breast cancer cells.
Neubauer
Hans
H
Department of Obstetrics and Gynaecology, University of Tuebingen, 72076 Tuebingen, Germany. hans.neubauer@med.uni-tuebingen.de
Ruoff
Alexandra
A
Paessler
Nicole
N
Solomayer
Erich
E
Wallwiener
Diethelm
D
Fehm
Tanja
T
D001485:Basement Membrane; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D024141:Collagen Type IV / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000096:biosynthesis* / Q000235:genetics; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D024141:Collagen Type IV; D047629:Estrogen Receptor beta; D007797:Laminin; D020033:Protein Isoforms; D012333:RNA, Messenger