title: Different strains of mice present distinct lung tissue mechanics and extracellular matrix composition in a model of chronic allergic asthma.
abstract: The impact of genetic factors on asthma is well recognized but poorly understood. We tested the hypothesis that different mouse strains present different lung tissue strip mechanics in a model of chronic allergic asthma and that these mechanical differences may be potentially related to changes of extracellular matrix composition and/or contractile elements in lung parenchyma. Oscillatory mechanics were analysed before and after acetylcholine (ACh) in C57BL/10, BALB/c, and A/J mice, subjected or not to ovalbumin sensitization and challenge. In controls, tissue elastance (E) and resistance (R), collagen and elastic fibres' content, and alpha-actin were higher in A/J compared to BALB/c mice, which, in turn, were more elevated than in C57BL/10. A similar response pattern was observed in ovalbumin-challenged animals irrespective of mouse strain. E and R augmented more in ovalbumin-challenged A/J [E: 22%, R: 18%] than C57BL/10 mice [E: 9.4%, R: 11%] after ACh In conclusion, lung parenchyma remodelled differently yielding distinct in vitro mechanics according to mouse strain.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, 21949-900, Rio de Janeiro, Brazil.
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Antunes
authors.forename: Soraia C
authors.initials: SC
authors.lastname: Abreu
authors.forename: Nilsa R
authors.initials: NR
authors.lastname: Damaceno-Rodrigues
authors.forename: Edwin R
authors.initials: ER
authors.lastname: Parra
authors.forename: Vera L
authors.initials: VL
authors.lastname: Capelozzi
authors.forename: Mariona
authors.initials: M
authors.lastname: Pinart
authors.forename: Pablo V
authors.initials: PV
authors.lastname: Romero
authors.forename: Patrícia M R
authors.initials: PM
authors.lastname: Silva
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Martins
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000276:immunology*; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology*; D066298:In Vitro Techniques; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D015656:Respiratory Mechanics / Q000235:genetics* / Q000276:immunology; D013045:Species Specificity
chemical_list: D010047:Ovalbumin