Selective chromofluorogenic sensing of heparin by using functionalised silica nanoparticles containing binding sites and a signalling reporter.
Heparin detective: Silica nanoparticles functionalised with ion-channel scaffolds were prepared and used for the chromofluorogenic sensing of heparin in aqueous environments (see figure). The surface of the nanoparticles was functionalised with polyamines (binding sites) and thiols. The reaction of a dye (squaraine) with the surface thiol groups was selectively inhibited by the coordination of heparin with the partly protonated polyamines.
Climent
Estela
E
Instituto de Química Molecular Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.
Calero
Pilar
P
Marcos
M Dolores
MD
Martínez-Máñez
Ramón
R
Sancenón
Félix
F
Soto
Juan
J
D001665:Binding Sites; D003124:Colorimetry / Q000379:methods; D003503:Cyclobutanes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006493:Heparin / Q000032:analysis*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D014867:Water / Q000737:chemistry
D003503:Cyclobutanes; D005456:Fluorescent Dyes; D010636:Phenols; D012996:Solutions; C480596:squaraine; D014867:Water; D012822:Silicon Dioxide; D006493:Heparin