title: Effect of age on cytokine response in an experimental model of osteomyelitis.
abstract: To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann-Whitney U tests were used (P < or = 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureus induced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureus group showed increased levels of: IL-10 in young rats, and IL-1beta in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.
authors.affiliation: Department of Surgery, University of Zaragoza, Spain. fegarcia@comz.org
authors.forename: Felícito
authors.initials: F
authors.lastname: García-Alvarez
authors.forename: Marta
authors.initials: M
authors.lastname: Navarro-Zorraquino
authors.forename: Angel
authors.initials: A
authors.lastname: Castro
authors.forename: José María
authors.initials: JM
authors.lastname: Grasa
authors.forename: Cristina
authors.initials: C
authors.lastname: Pastor
authors.forename: Marta
authors.initials: M
authors.lastname: Monzón
authors.forename: Ana
authors.initials: A
authors.lastname: Martínez
authors.forename: Ignacio
authors.initials: I
authors.lastname: García-Alvarez
authors.forename: Javier
authors.initials: J
authors.lastname: Castillo
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lozano
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D004195:Disease Models, Animal; D020007:Implants, Experimental / Q000382:microbiology; D008297:Male; D010019:Osteomyelitis / Q000097:blood / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000276:immunology
chemical_list: D016207:Cytokines