The oxidative stress caused by salinity in two barley cultivars is mitigated by elevated CO2.
Changes in antioxidant metabolism because of the effect of salinity stress (0, 80, 160 or 240 mM NaCl) on protective enzyme activities under ambient (350 micromol mol(-1)) and elevated (700 micromol mol(-1)) CO(2) concentrations were investigated in two barley cultivars (Hordeum vulgare L., cvs Alpha and Iranis). Electrolyte leakage, peroxidation, antioxidant enzyme activities [superoxide dismutase (SOD), EC 1.15.1.1; ascorbate peroxidase (APX), EC 1.11.1.11; catalase (CAT), EC 1.11.1.6; dehydroascorbate reductase (DHAR), EC 1.8.5.1; monodehydroascorbate reductase (MDHAR), EC 1.6.5.4; glutathione reductase (GR), EC 1.6.4.2] and their isoenzymatic profiles were determined. Under salinity and ambient CO(2), upregulation of antioxidant enzymes such as SOD, APX, CAT, DHAR and GR occurred. However, this upregulation was not enough to counteract all ROS formation as both ion leakage and lipid peroxidation came into play. The higher constitutive SOD and CAT activities together with a higher contribution of Cu,Zn-SOD 1 detected in Iranis might possibly contribute and make this cultivar more salt-tolerant than Alpha. Elevated CO(2) alone had no effect on the constitutive levels of antioxidant enzymes in Iranis, whereas in Alpha it induced an increase in SOD, CAT and MDHAR together with a decrease of DHAR and GR. Under combined conditions of elevated CO(2) and salinity the oxidative damage recorded was lower, above all in Alpha, together with a lower upregulation of the antioxidant system. So it can be concluded that elevated CO(2) mitigates the oxidative stress caused by salinity, involving lower ROS generation and a better maintenance of redox homeostasis as a consequence of higher assimilation rates and lower photorespiration, being the response dependent on the cultivar analysed.
Pérez-López
Usue
U
Departamento de Biología Vegetal y Ecología, Facultad de Ciencia y Tecnología, Universidad del País Vasco/EHU, Bilbao, Spain. usue.perez@ehu.es
Robredo
Anabel
A
Lacuesta
Maite
M
Sgherri
Cristina
C
Muñoz-Rueda
Alberto
A
Navari-Izzo
Flavia
F
Mena-Petite
Amaia
A
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D002245:Carbon Dioxide / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D001467:Hordeum / Q000187:drug effects* / Q000201:enzymology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D002245:Carbon Dioxide; D012965:Sodium Chloride; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase