Expression of four housekeeping proteins in elderly patients with schizophrenia.
We compared protein expression by Western blot analysis in four areas of postmortem brain from patients with schizophrenia and control subjects for several proteins that are often used as controls for Western blot studies: beta-tubulin, actin, glyceraldehyde-3-phosphate dehydrogenase, and valosin-containing protein. We did not detect any differences in expression between subjects with schizophrenia and a comparison group. These results suggest that all four proteins are suitable loading controls for postmortem studies of schizophrenia.
Bauer
Deborah Elaine
DE
Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, SC560, 1530 3rd Ave S., Birmingham, AL, 35294-0017, USA. debbauer@uab.edu
Haroutunian
Vahram
V
McCullumsmith
Robert E
RE
Meador-Woodruff
James H
JH
D000199:Actins / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000368:Aged; D000704:Analysis of Variance; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006179:Gyrus Cinguli / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D017397:Prefrontal Cortex / Q000378:metabolism; D012559:Schizophrenia / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D000074405:Valosin Containing Protein; D014793:Visual Cortex / Q000378:metabolism
D000199:Actins; D018797:Cell Cycle Proteins; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000251:Adenosine Triphosphatases; C000614178:VCP protein, human; D000074405:Valosin Containing Protein