Effects of two new steroids and cyproterone on some biomarkers of oxidative stress and serotonergic system on rat prostate and brain.
The purpose of this study was to evaluate the effect of cyproterone acetate (CPA, A) compared with new synthetic steroids 3alpha-acetoxy-5,6-epoxy-16-pregnen-20-one (B) and 17alpha-hydroxy-16beta-methyl-1,4,6-pregnatriene-3,20-dione (C) in rat prostate and brain. Groups of animals were treated either with A, B or C (4 mg kg(-1) day(-1)) by the intraperitoneal route for 5 days. Levels of reduced glutathione (GSH), 5-hydroxy-indole acetic acid (5-HIAA), lipid peroxidation (as thiobarbituric acid reactive substances, TBARS) and the activities of Na(+), K(+)- and total ATPases were assayed in prostate and brain for each group of animals including a control group. No appreciable changes were shown in Na(+), K(+)-ATPase and total ATPases and TBARS on prostate and brain of rats that received A, B and C steroids. However, the levels of GSH and 5-HIAA decreased significantly (P < 0.05) in both tissues for the steroids assayed. It is concluded that CPA and the homologues B and C steroids induce changes in the levels of GSH and serotonin in rat prostate and brain.
Calderón
G D
GD
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Mexico City, Mexico.
Bratoeff
E
E
Ramírez
L E
LE
Osnaya
B N
BN
García
A R
AR
Barragán
M G
MG
Hernández
G-E
GE
Juárez
O H
OH
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D017373:Cyproterone Acetate / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011281:Pregnatrienes / Q000494:pharmacology*; D011284:Pregnenolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
C539190:17-hydroxy-16-methyl-1,4,6-pregnatriene-3,20-dione; C539189:3-acetoxy-5,6-epoxy-16-pregnen-20-one; D015415:Biomarkers; D011281:Pregnatrienes; D017392:Thiobarbituric Acid Reactive Substances; D012701:Serotonin; D017373:Cyproterone Acetate; D006897:Hydroxyindoleacetic Acid; D011284:Pregnenolone; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione