The Center for Eukaryotic Structural Genomics.
The Center for Eukaryotic Structural Genomics (CESG) is a "specialized" or "technology development" center supported by the Protein Structure Initiative (PSI). CESG's mission is to develop improved methods for the high-throughput solution of structures from eukaryotic proteins, with a very strong weighting toward human proteins of biomedical relevance. During the first three years of PSI-2, CESG selected targets representing 601 proteins from Homo sapiens, 33 from mouse, 10 from rat, 139 from Galdieria sulphuraria, 35 from Arabidopsis thaliana, 96 from Cyanidioschyzon merolae, 80 from Plasmodium falciparum, 24 from yeast, and about 25 from other eukaryotes. Notably, 30% of all structures of human proteins solved by the PSI Centers were determined at CESG. Whereas eukaryotic proteins generally are considered to be much more challenging targets than prokaryotic proteins, the technology now in place at CESG yields success rates that are comparable to those of the large production centers that work primarily on prokaryotic proteins. We describe here the technological innovations that underlie CESG's platforms for bioinformatics and laboratory information management, target selection, protein production, and structure determination by X-ray crystallography or NMR spectroscopy.
Markley
John L
JL
Center for Eukaryotic Structural Genomics, Biochemistry Department, University of Wisconsin-Madison, Madison, WI 53706, USA. markley@nmrfam.wisc.edu
Aceti
David J
DJ
Bingman
Craig A
CA
Fox
Brian G
BG
Frederick
Ronnie O
RO
Makino
Shin-ichi
S
Nichols
Karl W
KW
Phillips
George N
GN
Primm
John G
JG
Sahu
Sarata C
SC
Vojtik
Frank C
FC
Volkman
Brian F
BF
Wrobel
Russell L
RL
Zolnai
Zsolt
Z
D000818:Animals; D018360:Crystallography, X-Ray; D023281:Genomics / Q000379:methods / Q000458:organization & administration*; D006801:Humans; D009096:Multi-Institutional Systems / Q000458:organization & administration; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D040901:Proteomics / Q000458:organization & administration
D011506:Proteins