Identification of Cistanche species by chemical and inter-simple sequence repeat fingerprinting.
Herba Cistanches is a common traditional Chinese medicine that has been used to reinforce the vital function of kidney and induce laxation for more than two thousands years. Four Cistanche species were found as Herba Cistanches in China herbal markets, including C. deserticola, C. tubulosa, C. salsa and C. sinensis. Phenylethanoid glycosides, particularly echinacoside and acteoside, are considered as active ingredients in Cistanche species. The contents of these compounds showed variation in different species and geographical sources. Standard chemical fingerprints were generated from each of four Cistanche species, which could be identification markers. In genetic analysis of Cistanche species, ninety-four inter-simple sequence repeat (ISSR) primers were used for polymerase chain reaction (PCR) amplification, and of which eight primers were found to be sufficient to distinguish different Cistanche species. As a result, the chemical fingerprint combined with the genetic fingerprint for distinction of Cistanche species could serve as markers for quality control of Herba Cistanches.
Shi
Hai Ming
HM
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, PR China.
Wang
Jing
J
Wang
Meng Yue
MY
Tu
Peng Fei
PF
Li
Xiao Bo
XB
D031671:Cistanche / Q000737:chemistry*; D016172:DNA Fingerprinting / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D018895:Microsatellite Repeats / Q000235:genetics*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification
D004365:Drugs, Chinese Herbal