[Genetic singularity coefficients of common vetch Vicia sativa L. accessions determined with molecular markers].
Organization and practical application of ex situ collections require estimation of genetic differences between numerous accessions of local cultivars and field weed forms collected from the same ecological and geographical region and similar in their morphophysiological characteristics. A mathematical algorithm for estimating the degree of genetic singularity of a specimen in the system of local gene pool determined with the help of molecular markers is described. The utility of this algorithm is demonstrated by the example of classification of 677 common vetch accessions from the collection of the Vavilov Institute of Plant Industry from 11 ecological-geographic regions of Russia analyzed using AFLP. The proposed classification of accessions is the result of processing the AFLP data by weighting the marker traits based on their frequency in particular regions. This allowed each accession to be characterized according to the ratio of rare and frequent alleles as a genetic singularity coefficient. The proposed method is appropriate for any types of molecular markers. A practical result of its application is the classification of accessions using a five-point score scale, which can be added to descriptors of certificate databases and used for optimization of the work with collections.
Potokina
E K
EK
Aleksandrova
T G
TG
D000465:Algorithms; D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D005819:Genetic Markers / Q000235:genetics; D010802:Phylogeny; D040641:Quantitative Trait Loci / Q000235:genetics*; D033022:Vicia sativa / Q000145:classification / Q000235:genetics*
D005819:Genetic Markers