[Molecular-genetic analysis of clonal intratumoral heterogeneity on colorectal adenocarcinomas].
We have examined the existence of intratumoral genetic heterogeneity for LOH on chromosomes 9p21 (p16, p15, p19), 13p14 (RB1), 10q23 (PTEN), 17p (TP53), microsatellite instability and K-RAS point mutations on four different segments of sporadic colorectal cancers. The intratumoral genetic heterogenity was detected in 9/11 (81%) colorectal adenocarcinomas and morphologically validated. These results show that colorectal cancer is highly heterogeneous for these molecular markers. Furthermore, the analysis has shown the order (succession) of the appearance of these molecular anomalies during tumorigenesis on sporadic CRC, and supposed, that K-RAS point mutations, and anomalies of p16-RB1-cyclin D pathway could occur before LOH on 10q23 (PTEN) and microsatellite instability during tumor progression.
Nemtsova
M N
MN
Pal'tseva
E M
EM
Babaian
A Iu
AIu
Mikhaĭlenko
D S
DS
Babenko
O V
OV
Samofalova
O Iu
OIu
Tsar'kov
P V
PV
Zaletaev
D V
DV
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D043171:Chromosomal Instability / Q000235:genetics; D002877:Chromosomes, Human / Q000235:genetics* / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D015689:Oncogene Protein p21(ras) / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism
C000614131:CDKN2A protein, human; D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D009363:Neoplasm Proteins; D016160:Retinoblastoma Protein; D015689:Oncogene Protein p21(ras)