Cytoprotective and antigenotoxic potential of Mangiferin, a glucosylxanthone against cadmium chloride induced toxicity in HepG2 cells.
Mangiferin (MGN), a glucosylxanthone present in large amounts in the leaves and edible mango fruits of Mangifera indica. Here, we report about MGN's potential for mitigating cadmium chloride (CdCl(2)) induced cytotoxic and genotoxic effects in HepG2 cells growing in vitro. The cytoprotective potential was assessed by MTT, clonogenic and apoptotic assays, while antigenotoxic effect by micronucleus and comet assay. The established cytotoxic and genotoxic effects were well indicated after CdCl(2) treatment and was mitigated by pretreatment with MGN. MGN prior to CdCl(2) treatment increased the cell survival (MTT), surviving fraction (clonogenic assay) and inhibited sub-G(1) population (flow cytometric analysis). Further, inhibition of CdCl(2) induced apoptotic cell death by MGN was confirmed by microscopic and DNA fragmentation assays. A significant (p<0.01) reduction in the micronuclei frequency and comet parameters after MGN pretreatment to CdCl(2) clearly indicated the antigenotoxic potential. Similarly, the reactive oxygen species generated by the CdCl(2) treatment were inhibited significantly (p<0.001) by MGN. Taken together, our study revealed that MGN has potent cytoprotective and antigenotoxic effect against CdCl(2) induced toxicity in HepG2 cell line and which may be attributed to decrease in CdCl(2) induced reactive oxygen species levels and resultant oxidative stress.
Satish Rao
B S
BS
Division of Radiobiology and Toxicology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka, India. satishraomlsc@gmail.com
Sreedevi
M V
MV
Nageshwar Rao
B
B
D016587:Antimutagenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D019256:Cadmium Chloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D020552:Comet Assay; D005434:Flow Cytometry; D006801:Humans; D015162:Micronucleus Tests; D008856:Microscopy, Fluorescence; D017382:Reactive Oxygen Species / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology*
D016587:Antimutagenic Agents; D017382:Reactive Oxygen Species; D044004:Xanthones; C013592:mangiferin; D019256:Cadmium Chloride