Enhanced release of chitosanase from Streptomyces griseus through direct interaction of liposome with cell membrane under heat stress.
A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41 degrees C increased the chitosanase production and release to 2.2 times higher than that at 37 degrees C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41 degrees C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41 degrees C, clearly showing the importance of the direct interaction between membrane and membrane.
Ngo
Kien Xuan
KX
Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Umakoshi
Hiroshi
H
Shimanouchi
Toshinori
T
Bui
Huong Thi
HT
Kuboi
Ryoichi
R
D002462:Cell Membrane / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006358:Hot Temperature; D008081:Liposomes; D013305:Streptomyces griseus / Q000201:enzymology*
D008081:Liposomes; D006026:Glycoside Hydrolases; C019868:chitosanase