Reactivity of German cockroach allergen, Bla g 2, peptide fragments to IgE antibodies in patients' sera.
Bla g 2 is a cockroach allergen of great importance. This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique. Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2). Only 5.3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients' sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies.
Lee
Haeseok
H
Department of Environmental Medical Biology and the Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
Jeong
Kyoung Yong
KY
Shin
Kwang Hyun
KH
Yi
Myung-hee
MH
Gantulaga
Darambazar
D
Hong
Chein-Soo
CS
Yong
Tai-Soon
TS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002648:Child; D003058:Cockroaches / Q000276:immunology; D000939:Epitopes; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008875:Middle Aged; D055815:Young Adult
D000906:Antibodies; D000939:Epitopes; D007073:Immunoglobulin E; D016282:Aspartic Acid Endopeptidases; C094699:allergen Bla g 2