Pomegranin, an antifungal peptide from pomegranate peels.
A new antifungal peptide designated as pomegranin, with an N-terminal sequence resembling that of rice disease resistance NB-S-LRR-like protein, was isolated from fresh pomegranate peels by ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Pomegranin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. It exhibited a molecular mass of 11 kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It inhibited mycelial growth in the fungi Botrytis cinerea and Fusarium oxysporum with an IC(50) of 2 microM and 6.1 microM, respectively. It was devoid of hemagglutinating, ribonuclease, deoxyribonuclease and protease inhibitory activities.
Guo
Guang
G
State Key Laboratory for Agrobiotechnology and College of Biological Science, China Agricultural University, Beijing, 100094, China.
Wang
He Xiang
HX
Ng
Tzi Bun
TB
D000595:Amino Acid Sequence; D020171:Botrytis / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005659:Fungicides, Industrial / Q000302:isolation & purification / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects; D029561:Lythraceae / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology*
D005659:Fungicides, Industrial; D010940:Plant Proteins; C538803:pomegranin protein, Punica granatum