title: Adipose overexpression of desnutrin promotes fatty acid use and attenuates diet-induced obesity.
abstract: OBJECTIVE
  To investigate the role of desnutrin in adipose tissue triacylglycerol (TAG) and fatty acid metabolism.
  
  
  METHODS
  We generated transgenic mice overexpressing desnutrin (also called adipose triglyceride lipase [ATGL]) in adipocytes (aP2-desnutrin) and also performed adenoviral-mediated overexpression of desnutrin in 3T3-L1CARDelta1 adipocytes.
  
  
  RESULTS
  aP2-desnutrin mice were leaner with decreased adipose tissue TAG content and smaller adipocyte size. Overexpression of desnutrin increased lipolysis but did not result in increased serum nonesterified fatty acid levels or ectopic TAG storage. We found increased cycling between diacylglycerol (DAG) and TAG and increased fatty acid oxidation in adipocytes from these mice, as well as improved insulin sensitivity.
  
  
  CONCLUSIONS
  We show that by increasing lipolysis, desnutrin overexpression causes reduced adipocyte TAG content and attenuation of diet-induced obesity. Desnutrin-mediated lipolysis promotes fatty acid oxidation and re-esterification within adipocytes.
authors.affiliation: Department of Nutritional Science and Toxicology, University of California, Berkeley, California, USA.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ahmadian
authors.forename: Robin E
authors.initials: RE
authors.lastname: Duncan
authors.forename: Krista A
authors.initials: KA
authors.lastname: Varady
authors.forename: Danubia
authors.initials: D
authors.lastname: Frasson
authors.forename: Marc K
authors.initials: MK
authors.lastname: Hellerstein
authors.forename: Andreas L
authors.initials: AL
authors.lastname: Birkenfeld
authors.forename: Varman T
authors.initials: VT
authors.lastname: Samuel
authors.forename: Gerald I
authors.initials: GI
authors.lastname: Shulman
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.forename: Chulho
authors.initials: C
authors.lastname: Kang
authors.forename: Hei Sook
authors.initials: HS
authors.lastname: Sul
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000273:Adipose Tissue / Q000502:physiology* / Q000503:physiopathology*; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D002149:Energy Intake; D005260:Female; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D009765:Obesity / Q000209:etiology / Q000517:prevention & control*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014304:Triolein / Q000378:metabolism; D000217:Acyltransferases
chemical_list: D002265:Carboxylic Ester Hydrolases; D008049:Lipase; D012313:RNA; D014304:Triolein; C494445:PNPLA2 protein, mouse; D000217:Acyltransferases