In vitro inhibitory effect of mesenchymal stem cells on zymosan-induced production of reactive oxygen species.
In vitro chemiluminescent test showed that human bone marrow mesenchymal stem cells and conditioned media dose-dependently inhibit production of reactive oxygen species by macrophages: 50% inhibition of chemiluminescence (compared to biocontrol) was observed at 1:1 mesenchymal stem cell/macrophage ratio or after addition of 20-25% conditioned media to the incubation medium. The observed mechanism of inhibition of production of reactive oxygen forms can play an essential role in the formation of local immunosuppressive microenvironment in the organism after allogenic transplantation of mesenchymal stem cells.
Tsyb
A F
AF
Medical Radiological Research Center, Russian Academy of Medical Sciences, Obninsk, Russia.
Petrov
V N
VN
Konoplyannikov
A G
AG
Saypina
E V
EV
Lepechina
L A
LA
Kalsina
S Sh
SSh
Semenkova
I V
IV
Agaeva
E V
EV
D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015054:Zymosan / Q000378:metabolism*
D017382:Reactive Oxygen Species; D015054:Zymosan