CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross-linking.
The ectoenzyme E-NPP3 (CD203c) has recently been identified as a novel activation-linked cell surface antigen on basophils. In the present study, we examined expression of CD203c on normal mast cells (MC)and bone marrow (bm) MC derived from 85 patients with systemic mastocytosis (SM), including cases with indolent SM (ISM, n=72), SM with associated clonal hematologic non-MC-lineage disease (SM-AHNMD, n=6), aggressive SM (ASM, n=3), and mast cell leukemia (MCL, n=4). Surface expression of CD203c was analyzed by multicolor flow cytometry. In patients with SM, bm MC expressed significantly higher amounts of CD203c compared to normal bm MC (median MFI in controls: 260 versus median MFI in SM: 516, p<0.05). Slightly lower amounts of CD203c were detected on MC in SM-AHNMD and ASM compared to ISM. To demonstrate CD203c expression in MC at the mRNA level, neoplastic MC were highly enriched by cell sorting, and were found to express CD203c mRNA in RT-PCR analysis. Cross-linking of the IgE receptor on MC resulted in a substantial upregulation of CD203c, whereas the KIT-ligand stem cell factor (SCF) showed no significant effects. In conclusion, CD203c is a novel activation-linked surface antigen on MC that is upregulated in response to IgE receptor cross-linking and is overexpressed on neoplastic MC in patients with SM.
Hauswirth
A W
AW
Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
Escribano
L
L
Prados
A
A
Nuñez
R
R
Mirkina
I
I
Kneidinger
M
M
Florian
S
S
Sonneck
K
K
Vales
A
A
Schernthaner
G-H
GH
Sanchez-Muñoz
L
L
Sperr
W R
WR
Bühring
H-J
HJ
Orfao
A
A
Valent
P
P
D001483:Base Sequence; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D008407:Mast Cells / Q000276:immunology*; D008415:Mastocytosis / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000276:immunology*; D011755:Pyrophosphatases / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D017455:Receptors, IgE / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D017931:DNA Primers; C427944:ENPP3 protein, human; D012333:RNA, Messenger; D017455:Receptors, IgE; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases