title: Carbon dioxide inhalation causes pulmonary inflammation.
abstract: The aim of this study was to assess whether one of the most common poisons of cellular respiration, i.e., carbon dioxide, is proinflammatory. CO(2) is naturally present in the atmosphere at the level of 0.038% and involved in numerous cellular biochemical reactions. We analyzed in vitro the inflammation response induced by exposure to CO(2) for 48 h (0-20% with a constant O(2) concentration of 21%). In vivo mice were submitted to increasing concentrations of CO(2) (0, 5, 10, and 15% with a constant O(2) concentration of 21%) for 1 h. The exposure to concentrations above 5% of CO(2) resulted in the increased transcription (RNase protection assay) and secretion (ELISA) of proinflammatory cytokines [macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, MIP-2, IL-8, IL-6, monocyte chemoattractant protein-1, and regulated upon activation, normal T cell expressed, and, presumably, secreted (RANTES)] by epithelial cell lines HT-29 or A549 and primary pulmonary cells retrieved from the exposed mice. Lung inflammation was also demonstrated in vivo by mucin 5AC-enhanced production and airway hyperreactivity induction. This response was mostly mediated by the nuclear translocation of p65 NF-kappaB, itself a consequence of protein phosphatase 2A (PP2A) activation. Short inhibiting RNAs (siRNAs) targeted toward PP2Ac reversed the effect of carbon dioxide, i.e., disrupted the NF-kappaB activation and the proinflammatory cytokine secretion. In conclusion, this study strongly suggests that exposure to carbon dioxide may be more toxic than previously thought. This may be relevant for carcinogenic effects of combustion products.
authors.affiliation: Service de Radiothérapie Hôpital Pitié-Salpétrière, 75013 Paris, France.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abolhassani
authors.forename: Adeline
authors.initials: A
authors.lastname: Guais
authors.forename: Philippe
authors.initials: P
authors.lastname: Chaumet-Riffaud
authors.forename: Annie J
authors.initials: AJ
authors.lastname: Sasco
authors.forename: Laurent
authors.initials: L
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics; D006801:Humans; D006935:Hypercapnia / Q000150:complications / Q000201:enzymology; D018836:Inflammation Mediators / Q000378:metabolism; D019570:Inhalation Exposure; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D011014:Pneumonia / Q000150:complications / Q000201:enzymology / Q000209:etiology*; D054648:Protein Phosphatase 2 / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D034741:RNA, Small Interfering; D002245:Carbon Dioxide; D054648:Protein Phosphatase 2