Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B.
Bacillus subtilis WU-S2B is a thermophilic dibenzothiophene (DBT)-desulfurizing bacterium and produces a flavin reductase (Frb) that couples with DBT and DBT sulfone monooxygenases. The recombinant Frb was purified from Escherichia coli cells expressing the frb gene and was characterized. The purified Frb exhibited high stability over wide temperature and pH ranges of 20-55 degrees C and 2-12, respectively. Frb contained FMN and exhibited both flavin reductase and nitroreductase activities.
Takahashi
Shusuke
S
Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Furuya
Toshiki
T
Ishii
Yoshitaka
Y
Kino
Kuniki
K
Kirimura
Kohtaro
K
D000327:Adsorption; D001412:Bacillus subtilis / Q000201:enzymology*; D001671:Biochemistry / Q000379:methods; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D038181:FMN Reductase / Q000737:chemistry*; D005415:Flavins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009601:Nitroreductases / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry; D013696:Temperature; D013876:Thiophenes / Q000737:chemistry*
D005415:Flavins; D011994:Recombinant Proteins; D013876:Thiophenes; D013455:Sulfur; D038181:FMN Reductase; D009601:Nitroreductases; C413454:flavin nitroreductase; C016366:dibenzothiophene