title: A human proteome detection and quantitation project.
abstract: The lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e.g. two peptides from the protein product of each of the approximately 20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization.
authors.affiliation: The Plasma Proteome Institute, Washington, D. C. 20009, USA. leighanderson@plasmaproteome.org
authors.forename: N Leigh
authors.initials: NL
authors.lastname: Anderson
authors.forename: Norman G
authors.initials: NG
authors.lastname: Anderson
authors.forename: Terry W
authors.initials: TW
authors.lastname: Pearson
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
authors.forename: Amanda G
authors.initials: AG
authors.lastname: Paulovich
authors.forename: Scott D
authors.initials: SD
authors.lastname: Patterson
authors.forename: Michael
authors.initials: M
authors.lastname: Gillette
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.forename: Steven A
authors.initials: SA
authors.lastname: Carr
mesh_terms: D015415:Biomarkers / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D010865:Pilot Projects; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics
chemical_list: D015415:Biomarkers; D020543:Proteome