title: A study of the ultrastructure of fragile-X-related proteins.
abstract: Fragile-X-related proteins form a family implicated in RNA metabolism. Their sequence is composed of conserved N-terminal and central regions which contain Tudor and KH domains and of a divergent C-terminus with motifs rich in arginine and glycine residues. The most widely studied member of the family is probably FMRP (fragile X mental retardation protein), since absence or mutation of this protein in humans causes fragile X syndrome, the most common cause of inherited mental retardation. Understanding the structural properties of FMRP is essential for correlating it with its functions. The structures of isolated domains of FMRP have been reported, but nothing is yet known with regard to the spatial arrangement of the different modules, partly because of difficulties in producing both the full-length protein and its multidomain fragments in quantities, purities and monodispersity amenable for structural studies. In the present study, we describe how we have produced overlapping recombinant fragments of human FMRP and its paralogues which encompass the evolutionary conserved region. We have studied their behaviour in solution by complementary biochemical and biophysical techniques, identified the regions which promote self-association and determined their overall three-dimensional shape. The present study paves the way to further studies and rationalizes the existing knowledge on the self-association properties of these proteins.
authors.affiliation: National Institute for Medical Research, The Ridgeway, London NW7 1AA, UK.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Sjekloća
authors.forename: Petr V
authors.initials: PV
authors.lastname: Konarev
authors.forename: John
authors.initials: J
authors.lastname: Eccleston
authors.forename: Ian A
authors.initials: IA
authors.lastname: Taylor
authors.forename: Dmitri I
authors.initials: DI
authors.lastname: Svergun
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pastore
mesh_terms: D002942:Circular Dichroism; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D017434:Protein Structure, Tertiary
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1