Identification of mutation sites on omega3 desaturase genes from Mortierella alpina 1S-4 mutants.
The mutation sites on omega3 desaturase genes in two omega3 desaturase-defective mutants derived from arachidonic acid-producing Mortierella alpina 1S-4 were identified. The mutations each resulted in an amino acid replacement (W232Stop or W386Stop) which caused a lack of omega3 desaturase activity in these mutants.
Sakuradani
Eiji
E
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Abe
Takahiro
T
Shimizu
Sakayu
S
D016718:Arachidonic Acid / Q000378:metabolism; D004252:DNA Mutational Analysis; D044943:Fatty Acid Desaturases / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry; D008954:Models, Biological; D008957:Models, Genetic; D020094:Mortierella / Q000235:genetics*; D009154:Mutation; D013696:Temperature; D013997:Time Factors
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007527:Isoenzymes; D016718:Arachidonic Acid; D044943:Fatty Acid Desaturases; C078565:omega-3 fatty acid desaturase