The chaotrope-soluble glycoprotein GP1 is a constituent of the insoluble glycoprotein framework of the Chlamydomonas cell wall.
Chlamydomonas reinhardtii wild-type cells are surrounded by the insoluble cell wall component, a sac-like framework of cross-linked glycoproteins containing 22% hydroxyproline. The chaotrope-soluble cell wall glycoprotein GP1 is the only polypeptide with an even higher proportion of hydroxyproline (35%) occurring in vegetative C. reinhardtii cells. Mass spectrometric analyses of peptides released from the purified insoluble cell wall fraction by trypsin treatment and epitope analyses of polyclonal antibodies raised against different deglycosylation products of this particular wall fraction using 181 chemically synthesized GP1-derived pentadecapeptides revealed evidence that GP1 is indeed a constituent of the insoluble wall component.
Voigt
Jürgen
J
Institute for Biochemistry, Charité, Paul Ehrlich Centre for Experimental Medicine, Berlin, Germany. juergen.voigt@charite.de
Frank
Ronald
R
Wöstemeyer
Johannes
J
D000595:Amino Acid Sequence; D000818:Animals; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility
D006023:Glycoproteins; D015800:Protozoan Proteins