title: Comparative proteomic analysis of human mesenchymal and embryonic stem cells: towards the definition of a mesenchymal stem cell proteomic signature.
abstract: Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue, we conducted a 2-DE gel analysis of mesenchymal stem cells isolated from bone marrow (BM), adipose tissue, synovial membrane and umbilical vein wall. We confirmed that BM and adipose tissue derived cells were very similar, which argue for their interchangeable use for cell therapy. We also compared human mesenchymal to embryonic stem cells and showed that umbilical vein wall stem cells, a neo-natal cell type, were closer to BM cells than to embryonic stem cells. Based on these proteomic data, we could propose a panel of proteins which were the basis for the definition of a mesenchymal stem cell proteomic signature.
authors.affiliation: CNRS, Institut de Génétique Humaine, UPR1142, Montpellier, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Roche
authors.forename: Bruno
authors.initials: B
authors.lastname: Delorme
authors.forename: Robert A J
authors.initials: RA
authors.lastname: Oostendorp
authors.forename: Romain
authors.initials: R
authors.lastname: Barbet
authors.forename: David
authors.initials: D
authors.lastname: Caton
authors.forename: Daniele
authors.initials: D
authors.lastname: Noel
authors.forename: Karim
authors.initials: K
authors.lastname: Boumediene
authors.forename: Helen A
authors.initials: HA
authors.lastname: Papadaki
authors.forename: Beatrice
authors.initials: B
authors.lastname: Cousin
authors.forename: Carole
authors.initials: C
authors.lastname: Crozet
authors.forename: Ollivier
authors.initials: O
authors.lastname: Milhavet
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.forename: Jacques
authors.initials: J
authors.lastname: Hatzfeld
authors.forename: Christian
authors.initials: C
authors.lastname: Jorgensen
authors.forename: Pierre
authors.initials: P
authors.lastname: Charbord
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D001853:Bone Marrow / Q000737:chemistry; D002478:Cells, Cultured; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D010641:Phenotype; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D013583:Synovial Membrane / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D015703:Antigens, CD; D011506:Proteins