Alternative procedures for analysis of lipid A modification with phosphoethanolamine or aminoarabinose.
MALDI-TOF mass spectrometry analysis of lipid A prepared using a Tri-reagent-based procedure with a 5-chloro-2-mercaptobenzothiazole matrix was preferable for the detection of phosphoethanolamine modification. In contrast, the analysis of lipid A prepared using an LPS extraction kit-based procedure with 2,5-dihydroxybenzoic acid was preferable for the detection of aminoarabinose modification.
Kawasaki
Kiyoshi
K
Faculty of Pharmaceutical Sciences, Doshisha Women's College, Kyotanabe, Kyoto 610-0395, Japan. kkawasak@dwc.doshisha.ac.jp <kkawasak@dwc.doshisha.ac.jp>
D001089:Arabinose / Q000031:analogs & derivatives* / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D004983:Ethanolamines / Q000737:chemistry*; D005841:Gentisates / Q000737:chemistry; D008050:Lipid A / Q000737:chemistry*; D013058:Mass Spectrometry; D012486:Salmonella typhimurium / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry
D052160:Benzothiazoles; D004983:Ethanolamines; D005841:Gentisates; D008050:Lipid A; D013438:Sulfhydryl Compounds; C016982:aminoarabinose; C005448:phosphorylethanolamine; D001089:Arabinose; C539674:5-chloro-2-mercaptobenzothiazole