title: Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source.
abstract: We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
authors.affiliation: Laboratoire de Photophysique Moléculaire, CNRS, Université Paris-Sud, Bat 210, 91405 Orsay, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Blandin
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lévêque-Fort
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lécart
authors.forename: Jack C
authors.initials: JC
authors.lastname: Cossec
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Potier
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Lenkei
authors.forename: Frédéric
authors.initials: F
authors.lastname: Druon
authors.forename: Patrick
authors.initials: P
authors.lastname: Georges
mesh_terms: D002460:Cell Line; D004867:Equipment Design; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D009446:Neurobiology / Q000379:methods; D055095:Optics and Photonics; D025941:Protein Interaction Mapping; D012031:Refractometry / Q000379:methods; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins