Isolation and properties of a chromosome-dependent KHR killer toxin in Saccharomyces cerevisiae.
A strain of the yeast Saccharomyces cerevisiae coding for KHR on the chromosome secreted a toxin that kills sensitive yeasts. The transformants of multicopy vectors carrying the KHR gene could secrete 3-4-fold the killer toxin of the donor strain. This toxic substance was purified 80-fold in specific activity from the culture filtrate by gel filtration and hydrophobic column chromatography. The purified toxin gave a single protein band with molecular mass of 20 kDa on SDS-PAGE and had an isoelectric point of pH 5.3. The toxin had novel killer activity against Candida glabrata and S. cerevisiae, but did not affect bacteria, fungi, or other yeasts.
Goto
K
K
National Research Institute of Brewing, 2-6-30 Takinogawa, Kita-ku, Tokyo 114, Japan.
Iwase
T
T
Kichise
K
K
Kitano
K
K
Totuka
A
A
Obata
T
T
Hara
S
S
D002850:Chromatography, Gel; D015825:Chromosomes, Fungal; D055646:Killer Factors, Yeast / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000302:isolation & purification; D014170:Transformation, Genetic
D055646:Killer Factors, Yeast; D029701:Saccharomyces cerevisiae Proteins; C065919:KHR protein, S cerevisiae