title: Structure of a polysaccharide from the lipopolysaccharide of Vibrio vulnificus CECT4602 containing 2-acetamido-2,3,6-trideoxy-3-[(S)- and (R)-3-hydroxybutanoylamino]-L-mannose.
abstract: A polysaccharide was isolated by GPC after mild acid treatment of the lipopolysaccharide of Vibrio vulnificus CECT4602 and found to contain L-Rha, D-GlcpNAc and 2-acetamido-2,3,6-trideoxy-3-(3-hydroxybutanoylamino)-L-mannose (L-RhaNAc3NHb). GLC analysis of the trifluoroacetylated (S)-2-octyl esters derived by full acid hydrolysis of the polysaccharide showed that approximately 80% of the 3-hydroxybutanoic acid has the S configuration and approximately 20% the R configuration. The following structure of the polysaccharide was established by (1)H and (13)C NMR spectroscopies, including 2D ROESY and (1)H/(13)C HMBC experiments: [carbohydrate sequence see in text].
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. knirel@ioc.a.ru
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Consuelo
authors.initials: C
authors.lastname: Esteve
authors.forename: Elena
authors.initials: E
authors.lastname: Alcaide
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000031:analogs & derivatives* / Q000737:chemistry*; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D041261:Vibrio vulnificus / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D008358:Mannose