title: Tissue damage-induced intestinal stem cell division in Drosophila.
abstract: Stem cell division is essential for tissue integrity during growth, aging, and pathogenic assaults. Adult gastrointestinal tract encounters numerous stimulations, and impaired tissue regeneration may lead to inflammatory diseases and cancer. Intestinal stem cells in adult Drosophila have recently been identified and shown to replenish the various cell types within the midgut. However, it is not known whether these intestinal stem cells can respond to environmental challenges. By feeding dextran sulfate sodium and bleomycin to flies and by expressing apoptotic proteins, we show that Drosophila intestinal stem cells can increase the rate of division in response to tissue damage. Moreover, if tissue damage results in epithelial cell loss, the newly formed enteroblasts can differentiate into mature epithelial cells. By using this newly established system of intestinal stem cell proliferation and tissue regeneration, we find that the insulin receptor signaling pathway is required for intestinal stem cell division.
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Alla
authors.initials: A
authors.lastname: Amcheslavsky
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
authors.forename: Y Tony
authors.initials: YT
authors.lastname: Ip
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001761:Bleomycin / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016264:Dextran Sulfate / Q000008:administration & dosage / Q000494:pharmacology; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007328:Insulin / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology* / Q000187:drug effects / Q000473:pathology*; D008954:Models, Biological; D011972:Receptor, Insulin / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D007328:Insulin; D001761:Bleomycin; D016264:Dextran Sulfate; D011972:Receptor, Insulin