title: An expanded clade of rodent Trim5 genes.
abstract: Trim5alpha from primates (including humans), cows, and rabbits has been shown to be an active antiviral host gene that acts against a range of retroviruses. Although this suggests that Trim5alpha may be a common antiviral restriction factor among mammals, the status of Trim5 genes in rodents has been unclear. Using genomic and phylogenetic analyses, we describe an expanded paralogous cluster of at least eight Trim5-like genes in mice (including the previously described Trim12 and Trim30 genes), and three Trim5-like genes in rats. Our characterization of the rodent Trim5 locus, and comparison to the Trim5 locus in humans, cows, and rabbits, indicates that Trim5 has undergone independent evolutionary expansions within species. Evolutionary analysis shows that rodent Trim5 genes have evolved under positive selection, suggesting evolutionary conflicts consistent with important antiviral function. Sampling six rodent Trim5 genes failed to reveal antiviral activities against a set of eight retroviral challenges, although we predict that such activities exist.
authors.affiliation: Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA.
authors.forename: Semih U
authors.initials: SU
authors.lastname: Tareen
authors.forename: Sara L
authors.initials: SL
authors.lastname: Sawyer
authors.forename: Harmit S
authors.initials: HS
authors.lastname: Malik
authors.forename: Michael
authors.initials: M
authors.lastname: Emerman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002415:Cats; D002460:Cell Line; D003001:Cloning, Molecular; D030541:Databases, Genetic; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D051381:Rats; D012190:Retroviridae / Q000502:physiology; D012192:Retroviridae Infections / Q000235:genetics; D012377:Rodentia / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D012641:Selection, Genetic; D016415:Sequence Alignment; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases
chemical_list: D002352:Carrier Proteins; C541473:TRIM5 protein, rat; D000070584:Tripartite Motif Proteins; C541472:TRIM5 protein, mouse; D044767:Ubiquitin-Protein Ligases