Characterization of two novel cry8 genes from Bacillus thuringiensis strain BT185.
Two novel cry8-type genes, cry8Ea1 and cry8Fa1, obtained from a Holotrichia parallela-specific Bacillus thuringiensis strain, BT185, were characterized. Findings showed that cry8Ea1 and cry8Fa1 encoded polypeptides of 1164 and 1174 amino acid residues, respectively. The deduced amino acid sequences of both Cry8Ea1 and Cry8Fa1 polypeptides are the most similar to that of Cry8Ba1. Eight conserved blocks (blocks 1-8) exist in Cry8Ea1 and Cry8Fa1 polypeptides compared with known Cry proteins. Cry8Ea1 and the Cry8Fa1 toxins could form spheric crystals when they were expressed in the acrystalliferous mutant strain HD73(-). The spores and crystals from the recombinant strain containing cry8Ea1 were toxic to Holotrichia parallela, with an LC(50) of 0.0875 x 10(8) colony-forming units (CFU)/g. However, Cry8Fa1 expressed in the recombinant strain was not toxic to H. parallela, Anomala corpulenta, or H. oblita.
Shu
Changlong
C
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Yu
Hong
H
Wang
Rongyan
R
Fen
Shuliang
S
Su
Xudong
X
Huang
Dafang
D
Zhang
Jie
J
Song
Fuping
F
D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D003001:Cloning, Molecular; D001517:Coleoptera / Q000187:drug effects* / Q000382:microbiology; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D016415:Sequence Alignment
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis