Expression of CYP1A1 and CYP1B1 in human endothelial cells: regulation by fluid shear stress.
OBJECTIVE
CYP1A1 and CYP1B1, members of the cytochrome P450 protein family, are regulated by fluid shear stress. This study describes the effects of duration, magnitude and pattern of shear stress on CYP1A1 and CYP1B1 expressions in human endothelial cells, towards the goal of understanding the role(s) of these genes in pro-atherogenic or anti-atherogenic endothelial cell functions.


RESULTS
We investigated CYP1A1 and CYP1B1 expressions under different durations, levels, and patterns of shear stress. CYP1A1 and CYP1B1 mRNA, protein, and enzymatic activity were maximally up-regulated at > or =24 h of arterial levels of shear stress (15-25 dynes/cm2). Expression of both genes was significantly attenuated by reversing shear stress when compared with 15 dynes/cm2 steady shear stress. Small interfering RNA knockdown of CYP1A1 resulted in significantly reduced CYP1B1 and thrombospondin-1 expression, genes regulated by the aryl hydrocarbon receptor (AhR). Immunostaining of human coronary arteries showed constitutive CYP1A1 and CYP1B1 protein expressions in endothelial cells. Immunostaining of mouse aorta showed nuclear localization of AhR and increased expression of CYP1A1 in the descending thoracic aorta, whereas reduced nuclear localization of AhR and attenuated CYP1A1 expression were observed in the lesser curvature of the aortic arch.


CONCLUSIONS
CYP1A1 and CYP1B1 gene and protein expressions vary with time, magnitude, and pattern of shear stress. Increased CYP1A1 gene expression modulates AhR-regulated genes. Based on our in vitro reversing flow data and in vivo immunostained mouse aorta, we suggest that increased expression of both genes reflects an anti-atherogenic endothelial cell phenotype.
Conway
Daniel E
DE
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University School of Medicine, 313 Ferst Drive, Suite 2116, Atlanta, GA 30332-0535, USA.
Sakurai
Yumiko
Y
Weiss
Daiana
D
Vega
J David
JD
Taylor
W Robert
WR
Jo
Hanjoong
H
Eskin
Suzanne G
SG
Marcus
Craig B
CB
McIntire
Larry V
LV
D000818:Animals; D001011:Aorta / Q000201:enzymology; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D050197:Atherosclerosis / Q000201:enzymology / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000201:enzymology; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D065633:Cytochrome P-450 CYP1B1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011673:Pulsatile Flow; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D012039:Regional Blood Flow; D013314:Stress, Mechanical; D019700:Thrombospondin 1 / Q000378:metabolism; D013997:Time Factors
D001189:Aryl Hydrocarbon Hydroxylases; D051792:Basic Helix-Loop-Helix Proteins; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1; D003577:Cytochrome P-450 Enzyme System; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018336:Receptors, Aryl Hydrocarbon; D019700:Thrombospondin 1; C111235:AHR protein, human; C484722:Ahr protein, mouse; C585496:CYP1B1 protein, human; C585498:Cyp1b1 protein, mouse