title: Laboratory detection of extended-spectrum beta-lactamase by an automated system.
abstract: This study aims to evaluate the positive predictive value (PPV) and the negative predictive value (NPV) of Vitek2 in detecting extended-spectrum beta-lactamase (ESBL) phenotypes when compared to a manual confirmatory test as gold standard. A sample of Escherichia coli, Klebsiella spp and Proteus mirabilis isolates were collected by 5 laboratories in the Emilia-Romagna Region (Italy). Vitek2 appears to be an accurate tool to detect ESBL phenotypes of E. coli isolates; some concern remains about its performance with the other bacterial species, especially P. mirabilis.
authors.affiliation: Area di Programma Rischio Infettivo, Agenzia Sanitaria e Sociale Regionale Emilia-Romagna, Viale Aldo Moro, 21-40127 Bologna, Italy. cgagliotti@regione.emilia-romagna.it
authors.forename: Carlo
authors.initials: C
authors.lastname: Gagliotti
authors.forename: Mario
authors.initials: M
authors.lastname: Sarti
authors.forename: Franca
authors.initials: F
authors.lastname: Benini
authors.forename: Antonio Paolo
authors.initials: AP
authors.lastname: Cipolloni
authors.forename: Giovanna
authors.initials: G
authors.lastname: Testa
authors.forename: Claudia
authors.initials: C
authors.lastname: Venturelli
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Moro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001331:Automation; D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases