The preparation, cytocompatibility, and in vitro biodegradation study of pure beta-TCP on magnesium.
Biodegradable and bioactive beta-tricalcium phosphate (beta-TCP) coatings were prepared on magnesium (Mg) in order to improve its biocompatibility by a chemical method. The tensile bonding strength of beta-TCP coating and Mg substrate was measured by the standard adhesion test (ISO 13779-4). And the cytocompatibility of beta-TCP coated Mg was studied by using human osteoblast-like MG63 cells. It was found that the MG63 cells could grow well on the surface of beta-TCP coated Mg and the cell viability on beta-TCP coated Mg was above 80% during the cocultivation of MG63 cells and beta-TCP coated Mg for 10 days, indicating no cytotoxicity. It was concluded that the beta-TCP coated Mg had good cytocompatibility. The degradation of Mg substrate with beta-TCP coating in vitro was studied in detail by XRD, EDX, SEM, and ICP. The results showed that a bone-like apatite continually formed on the surface of the sample with the degradation of both Mg substrate and beta-TCP coating in Hank's solution (a simulated body fluid). The biodegradation mechanism was preliminarily analyzed in the paper.
Geng
F
F
Institute of Metal Research, Chinese Academy of Sciences, Shenyang, People's Republic of China.
Tan
L L
LL
Jin
X X
XX
Yang
J Y
JY
Yang
K
K
D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008274:Magnesium / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013718:Tensile Strength; D014961:X-Ray Diffraction
D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D008274:Magnesium; C018392:tricalcium phosphate