title: An engineered Bacillus thuringiensis strain with insecticidal activity against Scarabaeidae (Anomala corpulenta) and Chrysomelidae (Leptinotarsa decemlineata and Colaphellus bowringi).
abstract: A genetically-engineered Bacillus thuringiensis (Bt) strain, 3A-HBF, with a broad insecticidal spectrum was constructed by introducing the recombinant plasmid pSTK-3A containing cry3Aa7 into the wild-type Bt strain HBF-1 containing the cry8Ca2 gene. The Cry3Aa7 protein produced by strain 3A-HBF was verified by SDS-PAGE and Western blotting. Flat rectangular crystals of Cry3Aa7 protein were observed besides spherical crystals (Cry8Ca2). The plasmid pSTK-3A was stable when strain 3A-HBF was grown in medium without antibiotics. The growth rate of 3A-HBF was not significantly different from that of the recipient strain, HBF-1. Strain 3A-HBF showed toxicity against two families of pests, Scarabaeidae and Chrysomelidae pests, which are susceptible to Cry8Ca (Anomala corpulenta) and Cry3Aa (Leptinotarsa decemlineata and Colaphellus bowringi). The 50% lethal concentrations of 3A-HBF against A. corpulenta, L. decemlineata and C. bowringi were 0.730 x 10(8) c.f.u./g dry soil, 1.74 microg/ml and 1.15 microg/ml, respectively.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China.
authors.forename: Ginxin
authors.initials: G
authors.lastname: Yan
authors.forename: Fuping
authors.initials: F
authors.lastname: Song
authors.forename: Changlong
authors.initials: C
authors.lastname: Shu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.forename: Chunqin
authors.initials: C
authors.lastname: Liu
authors.forename: Dafang
authors.initials: D
authors.lastname: Huang
authors.forename: Shuliang
authors.initials: S
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity*; D001517:Coleoptera / Q000382:microbiology*; D004731:Endotoxins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity*; D007300:Insect Control / Q000379:methods*; D007928:Lethal Dose 50; D010957:Plasmids; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis