title: Integrated microfluidic devices for combinatorial cell-based assays.
abstract: The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-microChip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-microChip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.
authors.affiliation: Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA.
authors.forename: Zeta Tak For
authors.initials: ZT
authors.lastname: Yu
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Kamei
authors.forename: Hiroko
authors.initials: H
authors.lastname: Takahashi
authors.forename: Chengyi Jenny
authors.initials: CJ
authors.lastname: Shu
authors.forename: Xiaopu
authors.initials: X
authors.lastname: Wang
authors.forename: George Wenfu
authors.initials: GW
authors.lastname: He
authors.forename: Robert
authors.initials: R
authors.lastname: Silverman
authors.forename: Caius G
authors.initials: CG
authors.lastname: Radu
authors.forename: Owen N
authors.initials: ON
authors.lastname: Witte
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Lee
authors.forename: Hsian-Rong
authors.initials: HR
authors.lastname: Tseng
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004867:Equipment Design; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis / Q000276:immunology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D041681:NIH 3T3 Cells; D019311:Staurosporine / Q000494:pharmacology; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D017304:Annexin A5; D000903:Antibiotics, Antineoplastic; D004791:Enzyme Inhibitors; D005353:Fibronectins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D003609:Dactinomycin; D051546:Cyclooxygenase 2; D019311:Staurosporine