Identification and characterization of two alternative sigma factors of RNA polymerase in the deep-sea piezophilic bacterium Shewanella violacea, strain DSS12.
Two genes for alternative sigma factors, sigma(E2) and sigma(E3), classified in the extracytoplasmic function sigma family for RNA polymerases, were identified in the deep-sea piezophilic bacterium Shewanella violacea DSS12. Amino acid alignments revealed that the domains for transcriptional functions were comparatively conserved compared with Escherichia coli sigma(E) in both proteins. Core-binding analysis suggested that both proteins function as sigma factors.
Kawano
Hiroaki
H
Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC). k23322000@yahoo.co.jp
Takahashi
Hiroyuki
H
Abe
Fumiyoshi
F
Kato
Chiaki
C
Horikoshi
Koki
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics; D005798:Genes, Bacterial; D016415:Sequence Alignment; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics; D012808:Sigma Factor / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014157:Transcription Factors
D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases