title: Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer.
abstract: An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4o degrees C after cell proliferation at 20 degrees C. The isolated bacterium was categorized as Gramnegative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.
authors.affiliation: Department of Microbiology, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Won-Hae
authors.initials: WH
authors.lastname: Lee
authors.forename: Ki-Woong
authors.initials: KW
authors.lastname: Cho
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.forename: Dong-Sun
authors.initials: DS
authors.lastname: Lee
authors.forename: Seon-Kap
authors.initials: SK
authors.lastname: Hwang
authors.forename: Seok-Jong
authors.initials: SJ
authors.lastname: Seo
authors.forename: Jong-Myeong
authors.initials: JM
authors.lastname: Kim
authors.forename: Sa-Youl
authors.initials: SY
authors.lastname: Ghim
authors.forename: Bang-Ho
authors.initials: BH
authors.lastname: Song
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee
authors.forename: Jong-Guk
authors.initials: JG
authors.lastname: Kim
mesh_terms: D002849:Chromatography, Gas; D004275:DNA, Ribosomal / Q000235:genetics; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis; D005798:Genes, Bacterial; D008563:Membrane Lipids / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020592:Shewanella / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D008563:Membrane Lipids; D012336:RNA, Ribosomal, 16S; D015118:Eicosapentaenoic Acid