Enzyme-linked immunosorbent assay for superoxide dismutase-excreted antigen in diagnosis of sylvatic and Andean cutaneous leishmaniasis of Peru.
A superoxide dismutase excreted by promastigote forms of L. (Viannia) peruviana (SODe-Lp), L. (Viannia) brazilensis (SODe-Lb), and L. (L.) amazonensis (SODe-La) is tested to evaluate its potential value as a diagnostic tool of mucocutaneous and Andean cutaneous leishmaniasis. We used 45 sera with mucocutaneous leishmaniasis (SL) and 68 with Andean cutaneous leishmaniasis (ACL). SODe-Lp antigen was recognized by 94% of the serum from ACL patients, and the SODe-Lb antigen was recognized by 93% of the serum from SL patients. Meanwhile, the result for SL and ACL patients with SODe-La antigen was 69% and 43% and SODe-Li was 11% and 9%, respectively. This suggest that antibodies to SODe-Lp undergo further response in patients with ACL and the antibodies to SODe-Lb do so preferentially in patients with SL. The SODe ELISA may be useful in endemic areas for discriminative assays between patients with different forms of leishmaniases and those with other clinical conditions.
Marín
Clotilde
C
Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
Longoni
Silvia S
SS
Urbano
Jesús
J
Minaya
Gloria
G
Mateo
Hector
H
de Diego
Jose A
JA
Rosales
María José
MJ
Pérez-Cordón
Gregorio
G
Romero
Desiré
D
Sánchez-Moreno
Manuel
M
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007525:Isoelectric Focusing; D007892:Leishmania braziliensis / Q000201:enzymology; D018314:Leishmania infantum / Q000201:enzymology; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000175:diagnosis*; D010568:Peru; D013482:Superoxide Dismutase / Q000032:analysis* / Q000097:blood
D000953:Antigens, Protozoan; D013482:Superoxide Dismutase