title: Immunosuppressive property of dried human amniotic membrane.
abstract: OBJECTIVE
  To report the immunosuppressive property of dry human amniotic membrane (dHAM).
  
  
  METHODS
  Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation.
  
  
  RESULTS
  dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1).
  
  
  CONCLUSIONS
  The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.
authors.affiliation: Department of Ophthalmology, Dongguk University, Koyang, South Korea.
authors.forename: Choul Yong
authors.initials: CY
authors.lastname: Park
authors.forename: Sahar
authors.initials: S
authors.lastname: Kohanim
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Peter L
authors.initials: PL
authors.lastname: Gehlbach
authors.forename: Roy S
authors.initials: RS
authors.lastname: Chuck
mesh_terms: D000650:Amnion / Q000276:immunology*; D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D003890:Desiccation; D006801:Humans; D007108:Immune Tolerance; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017252:CD3 Complex; C485468:Cd3e protein, mouse