Lymphocyte proliferation stimulated by activated human macrophages treated with Canova.
BACKGROUND
Canova (CA) is a homeopathic medication with immunomodulatory properties, recommended for patients with a depressed immune system. CA has been reported to increase in leukocyte numbers, cellular differentiation and reduction in tumor size.


OBJECTIVE
Since CA may stimulate lymphocyte differentiation, proliferation, and/or survival, the aim of the present study was to compare the mitotic index (MI) of phytohemagglutinin-stimulated human lymphocytes cultured in a medium supplemented with human macrophages activated by CA, with lymphocytes cultured in a medium without CA-treated macrophages.


RESULTS
In this study, the MI of lymphocyte cultured received the medium containing CA-stimulated macrophages showed a higher proliferation index (p<0.01) than the lymphocytes cultured in a medium without CA-treated macrophages. Our results suggest that CA treatment, in addition to activating macrophages, indirectly induces lymphocyte proliferation and has potential as a new adjuvant therapeutic approach.
Burbano
Rommel Rodriguez
RR
Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil. rommel@ufpa.br
Leal
Mariana Ferreira
MF
da Costa
Joana Borges
JB
Bahia
Marcelo de Olivieira
Mde O
de Lima
Patricia Danielle Lima
PD
Khayat
André Salim
AS
Seligman
Igor Chamon
IC
de Assumpção
Paulo Pimentel
PP
Buchi
Dorly de Freitas
Dde F
Smith
Marilia de Arruda Cardoso
Mde A
D000368:Aged; D002478:Cells, Cultured; D003435:Crotalid Venoms; D005260:Female; D005571:Formularies, Homeopathic as Topic; D006801:Humans; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts
C513575:Canova medication; D003435:Crotalid Venoms; D010936:Plant Extracts