title: Schistosoma mansoni infection alters co-stimulatory molecule expression and cell activation in asthma.
abstract: Chronic schistosomiasis induces Th2/T regulatory responses which are able to down-modulate allergic inflammation and asthma. Because co-stimulatory molecules and IL-10 are essential for inducing tolerance, the aim of this study was to determine by flow cytometry, the expression of CD28, CTLA4, CD40L, CD80, CD86, HLA-DR, IL-10 and IL-10 receptor, by mononuclear cells from asthmatic individuals infected with Schistosoma mansoni and compare with non-infected individuals. Peripheral blood mononuclear cells were stained with fluorochrome conjugated antibodies for the expression of co-stimulatory molecules, and for intracellular CTLA4 and IL-10 expression. There was no significant difference in the frequency of T cells expressing CD28 between the two groups. However, the frequency of TCD4(+) cells expressing CTLA4 and CD40L was higher in infected asthmatics. The frequency of monocytes expressing CD80 and CD86 did not differ between groups, while the expression of HLA-DR and IL-10 receptor was higher on monocytes of infected individuals. Furthermore, monocytes and CD4(+)CD25(+) cells of infected individuals expressed higher levels of IL-10. We conclude that, besides alternatively-activated monocytes that are, together with CD4(+)CD25(+) cells, important sources of IL-10, CTLA4 and CD40L expression may also participate in the down-modulation of inflammatory allergic response in S. mansoni-infected asthmatics.
authors.affiliation: Immunology Service, Federal University of Bahia, Brazil.
authors.forename: Ricardo R
authors.initials: RR
authors.lastname: Oliveira
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Gollob
authors.forename: Joanemile P
authors.initials: JP
authors.lastname: Figueiredo
authors.forename: Lêda M
authors.initials: LM
authors.lastname: Alcântara
authors.forename: Luciana S
authors.initials: LS
authors.lastname: Cardoso
authors.forename: Caroline S B
authors.initials: CS
authors.lastname: Aquino
authors.forename: Regis A
authors.initials: RA
authors.lastname: Campos
authors.forename: Maria Cecília
authors.initials: MC
authors.lastname: Almeida
authors.forename: Edgar M
authors.initials: EM
authors.lastname: Carvalho
authors.forename: Maria Ilma
authors.initials: MI
authors.lastname: Araujo
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D001249:Asthma / Q000150:complications* / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008213:Lymphocyte Activation; D008297:Male; D011971:Receptors, Immunologic / Q000096:biosynthesis; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D011971:Receptors, Immunologic