Fucoidan inhibits UVB-induced MMP-1 promoter expression and down regulation of type I procollagen synthesis in human skin fibroblasts.
UVB reduces type I procollagen levels and increases matrix metalloproteinases-1 (MMP-1) levels in human skin and plays a major role in the process of photoaging. We previously reported that fucoidan inhibits UVB-induced MMP-1 expression at the protein and mRNA levels in human skin fibroblasts (HS68). Yet, the effects of fucoidan on UVB-induced MMP-1 promoter activity and type I procollagen have not been investigated. In this study, we assessed the effects of fucoidan on the inhibition of MMP-1 promoter activity and on the increase of type I procollagen synthesis in human skin fibroblasts. Fucoidan treatment significantly inhibited MMP-1 promoter activity compared to UVB irradiation alone. Fucoidan treatment also increased type I procollagen mRNA and protein expression in a dose-dependent manner compared to the control. Our data indicate that fucoidan may prevent UVB-induced MMP-1 expression and inhibit down-regulation of type I procollagen synthesis. We suggest that fucoidan may be a potential therapeutic agent to prevent and treat skin photoaging.
Moon
Hee Jung
HJ
Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University, 34 Amnam-dong, Suh-gu, Busan, 602-702, Republic of Korea.
Lee
Sang Ho
SH
Ku
Mi Jeong
MJ
Yu
Byeng Chul
BC
Jeon
Man Joong
MJ
Jeong
Seok Hoon
SH
Stonik
Valentin A
VA
Zvyagintseva
Tatyana N
TN
Ermakova
Svetlana P
SP
Lee
Yong Hwan
YH
D000704:Analysis of Variance; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis*; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays
D024042:Collagen Type I; D011134:Polysaccharides; D012333:RNA, Messenger; C007789:fucoidan; D020781:Matrix Metalloproteinase 1