title: One tube multiplex PCR for simple screening of SCCmec I-V types of methicillin-resistant Staphylococcus aureus.
abstract: In this study, a rapid and simple one-tube multiplex PCR (M-PCR) method was developed for Staphylococcal Cassette Chromosome mec (SCCmec), primary screening. One hundred and eight methicillin-resistant Staphylococcus aureus (MRSA) isolates with SCC mec typing results using the Oliveira and de Lencastre method (Oliveira and de Lencastre. Antimicrob. Agents Chemother. 46:2155-2161, 2002), were evaluated for the efficacy of our method. Typing results were also compared to the recently published PCR protocol by Kondo et al., (Kondo et al., Antimicrob Agents Chemother. 51:264-74, 2007). The one-tube M-PCR method showed comparable efficacy to the other two methods. None of the isolates were classified as non-typable in Kondo's and our method. Two formerly assigned SCCmec type I isolates by Oliveira and de Lencastre were determined as SCCmec type IV by Kondo's and the method in this study. In conclusion, the one-tube M-PCR method developed in this study increased the rapidity and simplicity for primary screening SCCmec type I to V MRSA.
authors.affiliation: Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taipei, Taiwan.
authors.forename: Po-Liang
authors.initials: PL
authors.lastname: Lu
authors.forename: Jen-Chang
authors.initials: JC
authors.lastname: Chang
authors.forename: Hsiang-Ting
authors.initials: HT
authors.lastname: Hsu
authors.forename: Jiun-Han
authors.initials: JH
authors.lastname: Chen
authors.forename: Feng-Jui
authors.initials: FJ
authors.lastname: Chen
authors.forename: Sheng-Fung
authors.initials: SF
authors.lastname: Lin
authors.forename: L K
authors.initials: LK
authors.lastname: Siu
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial