Disruption of the novel gene fad104 causes rapid postnatal death and attenuation of cell proliferation, adhesion, spreading and migration.
The molecular mechanisms at the beginning of adipogenesis remain unknown. Previously, we identified a novel gene, fad104 (factor for adipocyte differentiation 104), transiently expressed at the early stage of adipocyte differentiation. Since the knockdown of the expression of fad104 dramatically repressed adipogenesis, it is clear that fad104 plays important roles in adipocyte differentiation. However, the physiological roles of fad104 are still unknown. In this study, we generated fad104-deficient mice by gene targeting. Although the mice were born in the expected Mendelian ratios, all died within 1 day of birth, suggesting fad104 to be crucial for survival after birth. Furthermore, analyses of mouse embryonic fibroblasts (MEFs) prepared from fad104-deficient mice provided new insights into the functions of fad104. Disruption of fad104 inhibited adipocyte differentiation and cell proliferation. In addition, cell adhesion and wound healing assays using fad104-deficient MEFs revealed that loss of fad104 expression caused a reduction in stress fiber formation, and notably delayed cell adhesion, spreading and migration. These results indicate that fad104 is essential for the survival of newborns just after birth and important for cell proliferation, adhesion, spreading and migration.
Nishizuka
Makoto
M
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
Kishimoto
Keishi
K
Kato
Ayumi
A
Ikawa
Masahito
M
Okabe
Masaru
M
Sato
Ryuichiro
R
Niida
Hiroyuki
H
Nakanishi
Makoto
M
Osada
Shigehiro
S
Imagawa
Masayoshi
M
D017667:Adipocytes / Q000378:metabolism / Q000502:physiology; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000502:physiology; D005328:Fetal Viability / Q000235:genetics*; D005353:Fibronectins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D018390:Gene Targeting; D051379:Mice; D018345:Mice, Knockout; D022502:Stress Fibers / Q000378:metabolism / Q000502:physiology
C538837:Fndc3b protein, mouse; D005353:Fibronectins