Membrane bound axin is sufficient for Wingless signaling in Drosophila embryos.
The Wingless signaling pathway controls various developmental processes in both vertebrates and invertebrates. Here I probe the requirement for nuclear localization of APC2 and Axin in the Wg signal transduction pathway during embryonic development of Drosophila melanogaster. I find that nuclear localization of APC2 appears to be required, but Axin can block signaling when tethered to the membrane. These results support the model where Axin regulates Armadillo localization and activity in the cytoplasm.
Tolwinski
Nicholas S
NS
Program in Developmental Biology, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA. tolwinsn@mskcc.org
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D060466:Axin Protein; D019521:Body Patterning; D002462:Cell Membrane / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D015398:Signal Transduction; D051155:Wnt1 Protein / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D060466:Axin Protein; C118000:Axn protein, Drosophila; D029721:Drosophila Proteins; D051155:Wnt1 Protein; C074622:wg protein, Drosophila