Evaluation of microvessel density (MVD) in canine mammary tumours by quantitative immunohistochemistry of the claudin-5 molecule.
In our recent investigation, angiogenesis was evaluated and quantified by immunohistochemical evaluation of microvessel density (MVD) using claudin-5 (CLDN-5) as a marker for vascular endothelium in 67 canine mammary gland tumours. Computer image analysis was used to measure the intratumoural MVD. Higher intratumoural MVD was detected in malignant simple neoplasms compared with benign tumours. Furthermore, the results of MVD were correlated with histological grade, higher grades being accompanied by higher MVD. In simple adenomas and grade I tubular-tubulopapillary simple carcinomas the intratumoural microvessels were wide and regular in shape with evident erythrocytes in their lumen. In grade III solid carcinomas the microvessels were smaller, less regular and had irregular shape, often without a distinct lumen, and isolated endothelial cells were frequently present. In the complex carcinomas MVD was low and the intratumoural microvessels were mostly irregular in shape without a distinct lumen. The evaluation of MVD by CLDN-5 immunohistochemistry may give useful additional information on the angiogenic potential of breast cancers in dogs.
Jakab
Csaba
C
Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent István University, H-1078 Budapest, István u. 2, Hungary. Jakab.Csaba@aotk.szie.hu
Halász
Judit
J
Kiss
András
A
Schaff
Zsuzsa
Z
Szász
Attila Marcell
AM
Rusvai
Miklós
M
Tóth
Zsolt Abonyi
ZA
Kulka
Janina
J
D000818:Animals; D002277:Carcinoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D007150:Immunohistochemistry / Q000662:veterinary*; D015674:Mammary Neoplasms, Animal / Q000098:blood supply*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*
D008565:Membrane Proteins