title: Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector.
abstract: A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kaneko
authors.forename: Takashi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Itaya
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005822:Genetic Vectors; D016680:Genome, Bacterial; D015698:Genomic Library; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D063151:Polycomb Repressive Complex 2; D011995:Recombination, Genetic; D014170:Transformation, Genetic
chemical_list: C093650:Jarid2 protein, mouse; D009419:Nerve Tissue Proteins; D063151:Polycomb Repressive Complex 2