title: Effect of mesenchymal stem cells and platelet-rich plasma on the healing of standardized bone defects in the alveolar ridge: a comparative histomorphometric study in minipigs.
abstract: OBJECTIVE
  The purpose of this study was to test the effect of the combination of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) incorporated into a fluorohydroxyapatite (FHA) scaffold on bone regeneration in cylindrical defects in the edentulous mandibular ridge of minipigs.
  
  
  METHODS
  Two mandibular premolar teeth were extracted bilaterally in 8 adult minipigs. After 2 months, 4 standardized defects of 3.5 mm diameter and 8 mm depth were created in each root site. The defects were randomly grafted with autogenous mandibular bone, FHA alone, PRP-FHA, or MSCs-PRP-FHA. A resorbable collagen membrane was placed over the defect area and the flaps were sutured. The animals were sacrificed 3 months later and biopsy samples were taken from the defect sites for histologic and histomorphometric assessment.
  
  
  RESULTS
  There was no evidence of inflammation or adverse tissue reaction with either treatment. MSCs-PRP-FHA-treated sites showed new vital bone between residual grafting particles. PRP-FHA- and FHA-treated sites showed residual particles in a background of marrow soft tissue with a moderate quantity of newly formed bone. Autogenous bone (46.97%) and MSCs-PRP-FHA (45.28%) produced a significantly higher amount of vital bone than PRP-FHA (37.95%), or FHA alone (36.03%). Further, the MSCs-PRP-FHA-treated defects showed a significantly higher percentage of contact between graft particles and newly formed bone compared with PRP-FHA and FHA group (59.23% vs 48.37% and 46.43%, respectively).
  
  
  CONCLUSIONS
  Our results suggest that, in this animal model, the addition of MSCs to PRP-FHA enhances bone formation after 3 months.
authors.affiliation: Department of Odontostomatological Sciences, University of Bologna, Bologna, Italy. checcopieri@yahoo.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Pieri
authors.forename: Enrico
authors.initials: E
authors.lastname: Lucarelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Corinaldesi
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Nicolò Nicoli
authors.initials: NN
authors.lastname: Aldini
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardino
authors.forename: Davide
authors.initials: D
authors.lastname: Donati
authors.forename: Claudio
authors.initials: C
authors.lastname: Marchetti
mesh_terms: D000539:Alveolar Process / Q000601:surgery*; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes; D016025:Bone Transplantation; D016557:Guided Tissue Regeneration, Periodontal; D006882:Hydroxyapatites; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; C116586:fluorohydroxyapatite