title: Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry.
abstract: The expression of interleukin-13 (IL13) following induction with IPTG in Escherichia coli results in metabolic changes as indicated by multi-parameter flow cytometry and traditional methods of fermentation profiling (O2 uptake rate, CO2 evolution rate and optical density measurements). Induction early in the rapid growth phase was optimal although this led to lower overall biomass concentrations and lower maximum specific growth rates. In contrast, induction in the mid-rapid growth phase was the most detrimental to cell quality as measured by cytoplamsic membrane depolarisation.
authors.affiliation: Department of Biochemical Engineering, University College London, Torrington Place, London WC1E7JE, UK.
authors.forename: Jennifer O
authors.initials: JO
authors.lastname: Shitu
authors.forename: John M
authors.initials: JM
authors.lastname: Woodley
authors.forename: Richard
authors.initials: R
authors.lastname: Wnek
authors.forename: Michel
authors.initials: M
authors.lastname: Chartrain
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hewitt
mesh_terms: D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000235:genetics; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D008564:Membrane Potentials; D010100:Oxygen / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D018793:Interleukin-13; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D007544:Isopropyl Thiogalactoside; D010100:Oxygen