Global map of SUMO function revealed by protein-protein interaction and genetic networks.
Systematic functional genomics approaches were used to map a network centered on the small ubiquitin-related modifier (SUMO) system. Over 250 physical interactions were identified using the SUMO protein as bait in affinity purification-mass spectrometry and yeast two-hybrid screens. More than 500 genes and 1400 synthetic genetic interactions were mapped by synthetic genetic array (SGA) analysis using eight different SUMO pathway query genes. The resultant global SUMO network highlights its role in 15 major biological processes and better defines functional relationships between the different components of the SUMO pathway. Using this information-rich resource, we have identified roles for the SUMO system in the function of the AAA ATPase Cdc48p, the regulation of lipid metabolism, localization of the ATP-dependent endonuclease Dna2p, and recovery from the DNA-damage checkpoint.
Makhnevych
Taras
T
Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S3E1, Canada.
Sydorskyy
Yaroslav
Y
Xin
Xiaofeng
X
Srikumar
Tharan
T
Vizeacoumar
Franco J
FJ
Jeram
Stanley M
SM
Li
Zhijian
Z
Bahr
Sondra
S
Andrews
Brenda J
BJ
Boone
Charles
C
Raught
Brian
B
D002467:Cell Nucleus; D002846:Chromatography, Affinity; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D053263:Gene Regulatory Networks; D005800:Genes, Fungal; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin