Hyphenated tools for phospholipidomics.
The analysis of intact and underivatised lipids in body fluids as well as in cell and tissue extracts is of utmost importance in the field of early diagnosis. Therefore, fast, reliable, and automated analytical methods are needed to detect known as well as unknown species. The combination of solid phase extraction, high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopy are best at meeting this challenge. Herein, we show a workflow for the reliable analysis of individual components in phosphatidylethanolamine extracts. The limitations and advantages of the individual methods are discussed.
Willmann
Jan
J
Department of Organic Chemistry, University of Bremen, Bremen, Germany.
Leibfritz
Dieter
D
Thiele
Herbert
H
D000818:Animals; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D010714:Phosphatidylethanolamines / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D010714:Phosphatidylethanolamines; D010743:Phospholipids; C483858:phosphatidylethanolamine