Transformation of L-tyrosine to L-dopa by a novel fungus, Acremonium rutilum, under submerged fermentation.
The present study deals with the transformation of L-tyrosine to L-dopa by Acremonium rutilum, a fungal tyrosinase producer, isolated from decomposed banana stud. This appears to be the first report on A. rutilum as a polyphenoloxidase producer with both cresolase and catecholase activity. Enriched Czapek-Dox agar was used for plate assay screening. Enriched potato dextrose broth was used for optimization studies, which induced high levels of L-dopa under submerged fermentation. A. rutilum gave the maximum L-dopa production (0.89 mg/ml) and tyrosinase activity (1095 U/mg) under the optimized parameters, that is, a temperature of 25 degrees C, pH 5.5, an inoculum size of 2.5 ml, and an incubation time of 72-120 h, with L-tyrosine (5 mg/ml) as substrate. Five resolved bands, with R(f) values of 0.73, 0.60, 0.54, 0.37, and 0.26, were observed, which confirmed the presence of L-dopa. This study involves the elevated profile of L-dopa production. Such study is needed, as L-dopa has the ability to control Parkinson's disease.
Krishnaveni
R
R
Department of Microbiology, Gulbarga University, Gulbarga, India. sanskruthi11@yahoo.com
Rathod
Vandana
V
Thakur
M S
MS
Neelgund
Y F
YF
D000164:Acremonium / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D046508:Culture Techniques; D005285:Fermentation; D005399:Fishes / Q000382:microbiology; D005656:Fungal Proteins / Q000378:metabolism; D007980:Levodopa / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D028521:Musa / Q000382:microbiology; D012988:Soil Microbiology; D013696:Temperature; D014443:Tyrosine / Q000378:metabolism*
D005656:Fungal Proteins; D014443:Tyrosine; D007980:Levodopa; D014442:Monophenol Monooxygenase