[TRPV5 and TRPV6 calcium channels in human T cells].
The recent cloning of the special calcium channels TRPV5 and TRPV6 (transient receptor potential vanilloid channels) provided the molecular base for the studying of new candidate of calcium influx in non-excitable cells. Using RT-PCR technique we obtained endogenous expression of the mRNAs trpv5 and trpv6 in lymphoblast leukemia Jurkat cells and in human blood primary T lymphocytes. Additionally, Western blot analysis showed TRPV5 proteins in both the whole lysate and in the crude membrane preparations from Jurkat cells and normal T lymphocytes. The using of the immunoprecipitation revealed TRPV6 proteins in Jurkat cells, whereas in normal T lymphocytes TRPV6 was not detected. The expression pattern and the selective Ca2+ permeation properties of TRPV5 and TRPV6 channels indicate an important role of these channels in the Ca2+ homeostasis and probably in malignant transformation of blood cells.
Vasil'eva
I O
IO
Neguliaev
Iu A
IuA
Marakhova
I I
II
Semenova
S B
SB
D015153:Blotting, Western; D015220:Calcium Channels / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000235:genetics*; D014158:Transcription, Genetic
D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C492239:TRPV5 protein, human; C120776:TRPV6 channel