Isolation and physiological characterization of Bacillus clausii SKAL-16 isolated from wastewater.
An alkaliphilic bacterium, Bacillus clausii SKAL-16, was isolated from soil that had been contaminated with vegetable oil. The optimal pH and general pH range for bacterial growth was 8, and 7 to 10, respectively. The bacterium could grow on tributyrin and glycerol, but could not grow on acetate and butyrate. The SKAL-16 strain excreted butyric acid during growth on tributyrin, and selectively ingested glycerol during growth on a mixture of butyric acid and glycerol. The SKAL-16 generated intracellular lipase, but did not produce esterase and extracellular lipase. The DNA fragment amplified with the chromosomal DNA of SKAL-16 and primers designed on the basis of the esterase-coding gene of Bacillus clausii KSM-K16 was not identical with the esterase-coding gene contained in the GenBank database. Pyruvate dehydrogenase, isocitrate dehydrogenase, and malate dehydrogenase activities were detected in the cellfree extract (crude enzyme).
Lee
Sung Hun
SH
Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea.
Park
Doo Hyun
DH
D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001483:Base Sequence; D002087:Butyrates / Q000378:metabolism; D002952:Citric Acid Cycle / Q000502:physiology; D004950:Esterases / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism; D008969:Molecular Sequence Data; D010938:Plant Oils; D015203:Reproducibility of Results; D012988:Soil Microbiology; D014280:Triglycerides / Q000378:metabolism; D014866:Waste Products
D002087:Butyrates; D010938:Plant Oils; D014280:Triglycerides; D014866:Waste Products; D004950:Esterases; D008049:Lipase; D005990:Glycerol; C005830:tributyrin