Biotic and abiotic stress down-regulate miR398 expression in Arabidopsis.
MicroRNA398 targets two Cu/Zn superoxide dismutases (CSD1 and CSD2) in higher plants. Previous investigations revealed both decreased miR398 expression during high Cu(2+) or paraquat stress and increased expression under low Cu(2+) or high sucrose in the growth medium. Here, we show that additional abiotic stresses such as ozone and salinity also affect miR398 levels. Ozone fumigation decreased miR398 levels that were gradually restored to normal levels after relieved from the stress. Furthermore, miR398 levels decreased in Arabidopsis leaves infiltrated with avirulent strains of Pseudomonas syringae pv. tomato, Pst DC3000 (avrRpm1 or avrRpt2) but not the virulent strain Pst DC3000. To our knowledge, miR398 is the first miRNA shown to be down-regulated in response to biotic stress (P. syringae). CSD1, but not CSD2, mRNA levels were negatively correlated with miR398 levels during ozone, salinity and biotic stress, suggesting that CSD2 regulation is not strictly under miR398 control during diverse stresses. Overall, this study further establishes a link between oxidative stress and miR398 in Arabidopsis.
Jagadeeswaran
Guru
G
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
Saini
Ajay
A
Sunkar
Ramanjulu
R
D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics; D015152:Blotting, Northern; D015536:Down-Regulation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D054884:Host-Pathogen Interactions; D007527:Isoenzymes / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D010126:Ozone / Q000494:pharmacology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D044224:Pseudomonas syringae / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics; D013997:Time Factors
D029681:Arabidopsis Proteins; D007527:Isoenzymes; C543882:MIRN398 microRNA, Arabidopsis; D035683:MicroRNAs; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D010126:Ozone; D013482:Superoxide Dismutase