Structural characterization and anti-fatigue activity of polysaccharides from the roots of Morinda officinalis.
Three polysaccharides MP-1, MP-2, and MP-3 were isolated from hot water extract of Chinese medicine Morinda officinalis through 95% ethanol precipitation and gel-filtration chromatography (DEAE-Sepharose CL-6B column and Sephadex G-75 or G-100 column). MP-1 was identified as an inulin-type fructan with simple linear (2-->1)-linked structure. Both MP-2 and MP-3 were acidic polysaccharides which consisted predominantly of galacturonic acid, arabinose and galactose. Partial structure characterization of MP-3 was carried out by partial acid hydrolysis and periodate oxidation. The total polysaccharides of the herb were tested in mice weight-loaded swimming model and were found to have anti-fatigue activity.
Zhang
Hua-Lin
HL
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, PR China.
Li
Jun
J
Li
George
G
Wang
Dong-mei
DM
Zhu
Long-ping
LP
Yang
De-po
DP
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005221:Fatigue / Q000097:blood / Q000188:drug therapy*; D006003:Glycogen / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D032066:Morinda / Q000737:chemistry*; D008517:Phytotherapy; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D013550:Swimming; D013997:Time Factors; D014508:Urea / Q000097:blood
D011134:Polysaccharides; D019344:Lactic Acid; D014508:Urea; D006003:Glycogen