Acetyl-l-carnitine inhibits TNF-alpha-induced insulin resistance via AMPK pathway in rat skeletal muscle cells.
In this study, we demonstrated effects of acetyl-l-carnitine (ALC) on insulin resistance induced by tumor necrosis factor-alpha (TNF-alpha) in rat L6 cells. TNF-alpha downregulated insulin-stimulated glucose uptake and increased Serine 307 phosphorylation of insulin receptor substrate-1 (IRS-1). However, the treatment of ALC improved insulin-stimulated glucose uptake via AMP-activated protein kinase (AMPK) activation in a dose-dependent manner. Together, our data suggest that ALC inhibits TNF-alpha-induced insulin resistance through AMPK pathway in skeletal muscle cells.
Zhang
Zhaofeng
Z
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, PR China.
Zhao
Ming
M
Li
Qiong
Q
Zhao
Haifeng
H
Wang
Junbo
J
Li
Yong
Y
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000108:Acetylcarnitine / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D007333:Insulin Resistance; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D012694:Serine / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology / Q000502:physiology
D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D014409:Tumor Necrosis Factor-alpha; D012694:Serine; D000108:Acetylcarnitine; D055372:AMP-Activated Protein Kinases; D005947:Glucose