Rare germline mutations in cyclin-dependent kinase inhibitor genes in multiple endocrine neoplasia type 1 and related states.
BACKGROUND
Germline mutation in the MEN1 gene is the usual cause of multiple endocrine neoplasia type 1 (MEN1). However, the prevalence of identifiable germline MEN1 mutations in familial MEN1 cases is only 70%. Some cases may have a germline mutation in another gene such as the p27 cyclin-dependent kinase inhibitor (CDKI).


OBJECTIVE
The aim of the study was to investigate cases of MEN1 or related states for germline mutations in all CDKI genes.


METHODS
A total of 196 consecutive index cases were selected with clear or suspected MEN1 and no identifiable germline MEN1 mutation. Every case was analyzed for germline mutation in each of the seven CDKI genes.


RESULTS
We identified benign polymorphisms of the CDKI genes and also 15 other initially unclassified sequence variants. After detailed gene/protein analysis, seven of these 15 variants were classified as probably pathological mutations. Three of these seven were probable mutations of p27. The remaining four were probable pathological mutations in three of the other CDKI genes, thereby implicating these three genes in the germline of human tumors. The identification rates for probably pathological mutations among the 196 index cases were similarly low for each of four CDKI genes: p15 (1%), p18 (0.5%), p21 (0.5%), and p27 (1.5%). No characteristic clinical subtype related to MEN1 was identified among the seven index cases and their families.


CONCLUSIONS
Rare germline mutation in any among four (p15, p18, p21, and p27) of the seven CDKIs is a probable cause of MEN1 or of some related states.
Agarwal
Sunita K
SK
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-1802, USA. SunitaA@mail.nih.gov
Mateo
Carmen M
CM
Marx
Stephen J
SJ
D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis / Q000235:genetics; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D018095:Germ-Line Mutation / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D018761:Multiple Endocrine Neoplasia Type 1 / Q000201:enzymology* / Q000235:genetics*; D010375:Pedigree; D012313:RNA / Q000235:genetics / Q000302:isolation & purification
D007527:Isoenzymes; D050760:Cyclin-Dependent Kinase Inhibitor p27; D012313:RNA; D004247:DNA; D018844:Cyclin-Dependent Kinases