title: [alpha1,2-fucosyltransferase gene transfection influences on biological behavior of ovarian carcinoma-derived RMG-I cells].
abstract: To explore the effect of alpha1,2-fucosyltransferase (alpha1,2-FT) gene on the biological behavior of ovarian carcinoma cells, the expression vector pcDNA3.1 -HFUT-H which containing human alpha1,2-FT gene coding region was constructed and transfected into ovarian carcinoma cell line RMG-I. alpha1,2-FT gene expression, Lewis y antigen expression, alpha1,2-FT activity, rates of cell growth and tumorogenesis in nude mice were compared before and after RMG-I being transfected. The transfection of alpha1,2-FT gene significantly increased the expression of alpha1,2-FT gene and Lewis y antigen in RMG-I cells. After the transfection, RMG-I cells showed remarkable improvement of alpha1,2-FT activity, cell proliferation, the ability of colony formation in soft agar and tumorogenesis in nude mice. The growth rates of alpha1,2-FT gene transfected RMG-I cells were obviously increased, presenting that the proportion of the cells in G2-M and S phases was obviously increased from 2.05% to 7.32% and from 23.95% to 33.27% respectively, and that of the cells in G1 phase was obviously decreased from 74.14% to 59.46%, as compared with the control group. These findings indicate that alpha1,2-FT and Lewis y antigen have the ability to enhance the proliferation and elevate the survival rates of RMG-I cells, which can promote the genesis and development of ovarian carcinoma.
authors.affiliation: Department of Gynecology and Obstetrics, The Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Ying Ying
authors.initials: YY
authors.lastname: Hao
authors.forename: Bei
authors.initials: B
authors.lastname: Lin
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yu Hua
authors.initials: YH
authors.lastname: Zhang
authors.forename: Fei Fei
authors.initials: FF
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Diao
authors.forename: Yang Ling
authors.initials: YL
authors.lastname: Ou
authors.forename: Shu Lan
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D005647:Fucosyltransferases