Genetically timed, activity-sensor and rainbow transsynaptic viral tools.
We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.
Boldogkoi
Zsolt
Z
Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged, H-6720, Hungary.
Balint
Kamill
K
Awatramani
Gautam B
GB
Balya
David
D
Busskamp
Volker
V
Viney
Tim James
TJ
Lagali
Pamela S
PS
Duebel
Jens
J
Pásti
Emese
E
Tombácz
Dóra
D
Tóth
Judit S
JS
Takács
Irma F
IF
Scherf
Brigitte Gross
BG
Roska
Botond
B
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D001921:Brain / Q000166:cytology / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009474:Neurons / Q000502:physiology / Q000821:virology; D009435:Synaptic Transmission / Q000502:physiology*; D013997:Time Factors; D014795:Visual Pathways / Q000502:physiology / Q000821:virology*; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein