The NtAMI1 gene functions in cell division of tobacco BY-2 cells in the presence of indole-3-acetamide.
Tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) cells can be grown in medium containing indole-3-acetamide (IAM). Based on this finding, the NtAMI1 gene, whose product is functionally equivalent to the AtAMI1 gene of Arabidopsis thaliana and the aux2 gene of Agrobacterium rhizogenes, was isolated from BY-2 cells. Overexpression of the NtAMI1 gene allowed BY-2 cells to proliferate at lower concentrations of IAM, whereas suppression of the NtAMI1 gene by RNA interference (RNAi) caused severe growth inhibition in the medium containing IAM. These results suggest that IAM is incorporated into plant cells and converted to the auxin, indole-3-acetic acid, by NtAMI1.
Nemoto
Keiichirou
K
Graduate School of Bioscience, Tokai University, 317 Nishino, Numazu, Shizuoka 410-0321, Japan.
Hara
Masamitsu
M
Suzuki
Masashi
M
Seki
Hikaru
H
Muranaka
Toshiya
T
Mano
Yoshihiro
Y
D000581:Amidohydrolases / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology; D017343:Genes, Plant / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D034622:RNA Interference; D014026:Nicotiana / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D003470:Culture Media; D007210:Indoleacetic Acids; C015950:indoleacetamide; D000581:Amidohydrolases; C058772:indole-3-acetamide hydrolase