title: Determination of protein composition and host-derived proteins of Bombyx mori nucleopolyhedrovirus by 2-dimensional electrophoresis and mass spectrometry.
abstract: OBJECTIVE
  The occlusion-derived form of baculovirus is specially adapted for primary infection of host midgut epithelial cells. The virion contains proteins essential for host-range determination and initiation of infection. Determination of protein composition of the occlusion-derived virus (ODV) will help us to understand this virion's infection mechanism.
  
  
  METHODS
  We obtained the ODV virion of Bombyx mori nucleopolyhedrovirus (BmNPV) by sucrose density gradient centrifugation, and used SDS-PAGE, 2-dimensional electrophoresis and mass spectrometry to identify--for the first time--proteins present within or associated with the ODV virion.
  
  
  RESULTS
  20 proteins, including 16 major proteins of the virion encoded by BmNPV and 4 proteins of the silkworm, were identified with confidence.
  
  
  CONCLUSIONS
  Three BmNPV proteins (orf40, P95 and protein tyrosine phosphatase), which had not been detected as components of ODV previously, were identified in this study. We propose that the host-derived proteins may be important for the assembly and maturation of the ODV capsid.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, PR China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Keya
authors.initials: K
authors.lastname: Cai
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D002499:Centrifugation, Density Gradient / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000032:analysis*; D013058:Mass Spectrometry; D017924:Nucleopolyhedroviruses / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D019476:Insect Proteins; D020543:Proteome