title: The use of components of plant origin in the development of production technology for live cold-adapted cultural influenza vaccine.
abstract: The impact of culturing conditions (multiplicity of cell culture infection with influenza virus, composition of growth and maintenance nutrient media) for the efficiency of multiplication of cold-adapted reassortant vaccine strains of influenza A and B viruses was evaluated. Soybean hydrolysate protein-based biological additive to nutrient medium provided effective reproduction of influenza A virus in MDCK cells in the presence of 2 microg/ml trypsin. The use of soybean peptone-based stabilizer provided retention of infectious titer of influenza virus grown in MDCK culture after its lyophilization to a level of 8.5 lg EID50/ml.
authors.affiliation: Vector Center of Virology and Biotechnology, Koltsovo, Novosibirsk region, Russia. mazurkova@vector.nsc.ru
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: T D
authors.initials: TD
authors.lastname: Kolokol'tsova
authors.forename: E A
authors.initials: EA
authors.lastname: Nechaeva
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
authors.forename: A N
authors.initials: AN
authors.lastname: Sergeev
mesh_terms: D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D004285:Dogs; D005260:Female; D006801:Humans; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000517:prevention & control*; D009975:Orthomyxoviridae / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry; D014779:Virus Replication
chemical_list: D007252:Influenza Vaccines; D010940:Plant Proteins