title: Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain.
abstract: The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of nerve growth factor-beta, are present in the tunnel and that competition for binding relates to the restricted space inside the propeller.
authors.affiliation: MIND Centre, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Arhus C, Denmark.
authors.forename: Esben M
authors.initials: EM
authors.lastname: Quistgaard
authors.forename: Peder
authors.initials: P
authors.lastname: Madsen
authors.forename: Morten K
authors.initials: MK
authors.lastname: Grøftehauge
authors.forename: Poul
authors.initials: P
authors.lastname: Nissen
authors.forename: Claus M
authors.initials: CM
authors.lastname: Petersen
authors.forename: Søren S
authors.initials: SS
authors.lastname: Thirup
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D008024:Ligands; D008322:Mammals; D008958:Models, Molecular; D009474:Neurons / Q000502:physiology; D009496:Neurotensin / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D000099183:Sortilin
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D008024:Ligands; D009496:Neurotensin; D000099183:Sortilin