[Generation and immunogenicity of a recombinant adenovirus expressing the E2 protein of classical swine fever virus in rabbits].
Classical swine fever (CSF), which is caused by classical swine fever virus (CSFV), causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. In this study, a recombinant replication-defective human adenovirus expressing the CSFV E2 gene (rAdV-E2) was generated and evaluated for the immunogenicity in rabbits. The results showed that the rabbits immunized with rAdV-E2 developed high-level CSFV-specific antibodies. The rAdV-E2-immunized rabbits were all free of the regular fever and the viral replication in the spleen upon challenge with C-strain, which were seen in the rabbits immunized with the parent adenovirus of rAdV-E2. This indicates that the recombinant adenovirus can be an attractive candidate vaccine against CSF.
Sun
Yuan
Y
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15000, China.
Qi
Qiaofen
Q
Liang
Bingbing
B
Cheng
Dan
D
Li
Na
N
Yu
Moyang
M
Wang
Yufei
Y
Liu
Nihong
N
Zhu
Qinghu
Q
Qiu
Huaji
H
D000256:Adenoviridae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005822:Genetic Vectors / Q000235:genetics; D007114:Immunization; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus