title: Epidemiological data of different human papillomavirus genotypes in cervical specimens of HIV-1-infected women without history of cervical pathology.
abstract: OBJECTIVE
  To study the epidemiology of different human papillomavirus (HPV) genotypes in cervical samples of HIV-1-infected women with normal Papanicolau smears.
  
  
  METHODS
  : Retrospective analysis of a prospective cohort.
  
  
  METHODS
  We selected HIV-1-infected women with 2 consecutive normal Papanicolau smears at baseline and at least 1 baseline and 1 follow-up cervical sample. HPV infection was assessed by second-generation hybrid capture (HC-2) and multiplex polymerase chain reaction (mPCR). HPV genotypes were determined by mPCR.
  
  
  RESULTS
  From a cohort of 139 women followed up to 4 years, 93 women meeting the inclusion criteria were analyzed. The mean period between samples was 20 months (range, 6-44 months). HPV baseline prevalence was 63% [59/93; 95% confidence interval (CI), 53% to 73%] using polymerase chain reaction and 41% (38/93; 95% CI, 31% to 51%) using HC-2, P = 0.007 (kappa, 0.45; P = 0.001). The most prevalent high oncogenic risk genotypes (HR-HPV) were HPV-16 (28%), HPV-33 (18%), HPV-52 (12%), HPV-58 (11%), and HPV-39 (11%). Infection with multiple HPV genotypes was detected in >40% of women. HPV infection persisted at follow-up in 86% (51/59; 95% CI, 77% to 95%) by polymerase chain reaction and 76% (29/38; 95% CI, 62% to 90%) by HC-2. HPV infection persisted in 55% of women with samples available beyond 3 years. The actuarial probabilities of clearance and incidence of HPV infection at 36 months were 16% and 45%, respectively.
  
  
  CONCLUSIONS
  HPV infection is highly prevalent and persistent among HIV-1-infected women with normal Papanicolau smears. HR-HPV genotypes other than HPV-16 (HPV-33, HPV-52) are frequently detected in HIV-infected women. mPCR provides better surveillance of HPV infection than HC-2 methods.
authors.affiliation: Lluita Contra La SIDA Foundation, Spain.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Videla
authors.forename: Laila
authors.initials: L
authors.lastname: Darwich
authors.forename: Maria Paz
authors.initials: MP
authors.lastname: Cañadas
authors.forename: Roger
authors.initials: R
authors.lastname: Paredes
authors.forename: Antoni
authors.initials: A
authors.lastname: Tarrats
authors.forename: Eva
authors.initials: E
authors.lastname: Castella
authors.forename: Mariona
authors.initials: M
authors.lastname: Llatjos
authors.forename: Margarita
authors.initials: M
authors.lastname: Bofill
authors.forename: Bonaventura
authors.initials: B
authors.lastname: Clotet
authors.forename: Guillem
authors.initials: G
authors.lastname: Sirera
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D000328:Adult; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications* / Q000453:epidemiology / Q000821:virology; D015497:HIV-1; D006801:Humans; D015994:Incidence; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D015347:RNA Probes; D002577:Uterine Cervical Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D014626:Vaginal Smears; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D015347:RNA Probes