Alternative splice variants of rat 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase-4 mRNA.
Expression of 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-4 (PFKFB-4) mRNA was studied in different rat organs. Several new unique alternative splice variants of PFKFB-4 mRNA were identified. They have deletions or inserts in fructose-2,6-bisphosphatase region as well as different length and amino acid sequence of C-terminal part. However, 6-phosphofructo-2-kinase catalytic domains were identical in all variants. Moreover, the expression of different alternative splice variants of PFKFB-4 mRNA has shown tissue specificity. Expression of both alternative splice variants of PFKFB-4 mRNA significantly changed in rats treated by methyl tretbutyl ether, ecologically dangerous chemical compound. Results of this investigation indicate a possible role of PFKFB-4 splice isoforms in cell-specific regulation of glycolysis and demonstrate the sensitivity of the regulation of alternative splicing to the action of toxic chemical compounds, in particular methyl tertbutyl ether.
Minchenko
D O
DO
Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv. ominchenko@yahoo.com
Mykhalchenko
V G
VG
Tsuchihara
K
K
Kanehara
S
S
Yavorovsky
O P
OP
Zavgorodny
I V
IV
Paustovsky
Yu O
YO
Komisarenko
S V
SV
Esumi
H
H
Minchenko
O H
OH
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D004785:Environmental Pollutants / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008738:Methyl Ethers / Q000633:toxicity; D008969:Molecular Sequence Data; D009928:Organ Specificity; D025481:Phosphofructokinase-2 / Q000235:genetics*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004785:Environmental Pollutants; D008738:Methyl Ethers; D020033:Protein Isoforms; D012333:RNA, Messenger; C043243:methyl tert-butyl ether; D025481:Phosphofructokinase-2