[Association of INF-gamma/A+874T gene polymorphisms with respiratory syncytial virus bronchiolitis].
OBJECTIVE
A deficient interferon-gamma (IFN-gamma) response has been involved in the pathogenesis of severe respiratory syncytial virus (RSV) infection. Gene polymorphisms in IFN-gamma/A+874T have been associated with the susceptibility to asthma and might be related to disease severity of RSV infection. This study investigated the single nucleotide polymorphisms (SNPs) of IFN-gamma/A+874T in Han children in Wenzhou area and to explore the correlation between gene polymorphisms of IFN-gamma/A+874T and the susceptibility and disease severity of RSV bronchiolitis, as well as the effect of SNPs upon nasopharyngeal secretions (NPS) IFN-gamma and total serum IgE levels.


METHODS
One hundred and fourteen hospitalized children with RSV bronchiolitis and 90 healthy controls were recruited. Sequence analysis was used for detecting the SNPs of IFN-gamma/A+874T. NPS IFN-gamma levels were measured using ELISA. Total serum IgE levels were assayed using the chemiluminescence method.


RESULTS
IFN-gamma/A+874T gene polymorphisms were present in both the patient and the control groups. AA and AT genotypes were found in both groups, with a AA frequency of 82.5% vs 77.8% and a AT frequency of 17.5% vs 21.1% (p>0.05). The frequency of allele was 90.4% (A) and 9.6% (T) in the patient group, and 88.3% (A) and 11.7% (T) in the control group, respectively. There were no significant differences in the allele frequency between the two groups. Moreover, no difference was found both in NPS IFN-gamma and total serum IgE levels between AA and AT genotypes in the patient group. There were no significant differences in the variation of IFN-gamma/+874 between mild and moderate to severe cases.


CONCLUSIONS
IFN-gamma/A+874T gene polymorphisms were present in Han children in Wenzhou area. Gene variations were not associated with the susceptibility and disease severity of RSV bronchiolitis as well as IFN-gamma and total serum IgE levels.
Dong
Lin
L
Department of Pulmonology, Yuying Children's Hospital Affiliated to Wenzhou Medical College, Wenzhou, Zhejiang 325027, China. donglin0728@vip.sina.com
Huang
Zhi-Ying
ZY
Chen
Xiao-Fang
XF
Zhou
Xiao-Cong
XC
Li
Jin-Yan
JY
Lin
Jian
J
D001988:Bronchiolitis / Q000235:genetics* / Q000276:immunology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D007371:Interferon-gamma / Q000235:genetics*; D008297:Male; D009305:Nasopharynx / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D018357:Respiratory Syncytial Virus Infections / Q000235:genetics* / Q000276:immunology
D007073:Immunoglobulin E; D007371:Interferon-gamma