Immune responses, ROS generation and the haemocyte damage of scallop Chlamys farreri exposed to Aroclor 1254.
The toxic effects of Aroclor 1254 (0.05, 0.5, 5 and 50 microg l(-1)) on scallop (Chlamys farreri) immune system in vivo were studied. The results showed that Aroclor 1254 had significant toxic effect on the parameters tested in this paper (P<0.05). The total number of haemocytes, the proportion of granulocytes, phagocytosis in all groups as well as the lysosomal membrane stability (LMS) in 5, 50 microg l(-1) and bacteriolytic activity 0.5, 5, 50 microg l(-1) treatments decreased significantly, while the proportion of hyalinocytes and the production of O2(-) in all treatments remarkably increased during the sampling time and tended to be stable gradually after 6-15 d. The bacteriolytic activity in 0.05 microg l(-1) treatments, LMS in 0.05, 0.5 microg l(-1) groups and the DNA damage (comet ratios and arbitrary values) in all treatments increased at the beginning of exposure and reached their peaks on day 1, day 1, day 6 and day 3, following that they all decreased gradually and became stable after 9-15 d. When the indices reached stability, except for DNA damage was higher than controls, the others were all significantly lower than those of controls (P<0.05). Thus, Aroclor 1254 has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms. Also it supports the speculation that the PCBs pollution is one of the important reasons of the mass mortality of the C. farreri.
Liu
Jing
J
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
Pan
Lu-Qing
LQ
Zhang
Lin
L
Miao
Jingjing
J
Wang
Jing
J
D000818:Animals; D001433:Bacteriolysis / Q000187:drug effects; D001772:Blood Cell Count; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D006434:Hemocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D008247:Lysosomes / Q000187:drug effects; D008837:Micrococcus / Q000502:physiology; D049897:Pectinidae / Q000187:drug effects* / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D020111:Chlorodiphenyl (54% Chlorine)