Application of a novel bone osteotomy plate leads to reduction in heat-induced bone tissue necrosis in sheep.
Previous studies have shown substantial effect thermal damage can have on new bone formation following osteotomy. In this study we evaluated the extent of thermal damage which occurs in four different methods of osteotomy and the effects it can have on bone healing. We further wanted to test whether a special osteotomy plate we constructed can lead to diminished heat generation during osteotomy and enhanced bone healing. The four methods evaluated included osteotomy performed by chisel, a newly constructed osteotomy plate, Gigly and oscillating saw. Twelve adult sheep underwent osteotomy performed on both tibiae. Bone fragments were stabilized using a fixation plate. Callus size was assessed using standard radiographs. Densitometry and histological evaluation were performed at 8 weeks following osteotomy. Temperature measurements were performed both in vivo during the operation, and ex vivo on explanted tibiae. The defects healed without complications and showed typical course of secondary fracture healing with callus ingrowth into the osteotomy gap. Radiographic examination of bone healing showed a tendency towards more callus formation in bones osteotomized using Gigly and oscillating saw, but this difference lacked significance. Use of Gigly and oscillating saw elicited much higher temperatures at the bone cortex surface, which subsequently lead to slightly impaired bone healing according to histological analysis. BMD was equal among all bones. In conclusion, the time required for complete healing of the defect differed depended greatly on the instruments used. The newly constructed osteotomy plate showed best results based on histological findings of capillary and osteoblast density.
Bekić
Marijo
M
Department of Surgery and Traumatology, General Hospital "Dubrovnik", Dubrovnik, Croatia. marijo.bekic@du.htnet.hr
Davila
Slavko
S
Hrskanović
Mato
M
Bekić
Marijana
M
Seiwerth
Sven
S
Erdeljić
Viktorija
V
Capak
Darko
D
Butković
Vladimir
V
D000818:Animals; D001860:Bone Plates; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000293:injuries / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D017102:Fracture Healing; D006358:Hot Temperature / Q000009:adverse effects*; D007431:Intraoperative Complications / Q000000981:diagnostic imaging / Q000473:pathology / Q000517:prevention & control*; D009336:Necrosis; D010027:Osteotomy / Q000009:adverse effects* / Q000295:instrumentation* / Q000379:methods; D011474:Prosthesis Design; D011859:Radiography; D012756:Sheep