A human proteome detection and quantitation project.
The lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e.g. two peptides from the protein product of each of the approximately 20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization.
Anderson
N Leigh
NL
The Plasma Proteome Institute, Washington, D. C. 20009, USA. leighanderson@plasmaproteome.org
Anderson
Norman G
NG
Pearson
Terry W
TW
Borchers
Christoph H
CH
Paulovich
Amanda G
AG
Patterson
Scott D
SD
Gillette
Michael
M
Aebersold
Ruedi
R
Carr
Steven A
SA
D015415:Biomarkers / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D010865:Pilot Projects; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics
D015415:Biomarkers; D020543:Proteome