title: Involvement of TMEM22 overexpression in the growth of renal cell carcinoma cells.
abstract: In order to clarify the molecular mechanism involved in renal carcinogenesis, and to identify molecular targets for development of novel treatments of renal cell carcinoma (RCC), we previously analyzed genome-wide gene expression profiles of clear-cell types of RCC by cDNA microarray. Among the transcativated genes, we herein focused on functional significance of TMEM22 (transmembrane protein 22), a transmembrane protein, in cell growth of RCC. Northern blot and semi-quantitative RT-PCR analyses confirmed up-regulation of TMEM22 in a great majority of RCC clinical samples and cell lines examined. Immunocytochemical analysis validated its localization at the plasma membrane. We found an interaction between TMEM22 and RAB37 (Ras-related protein Rab-37), which was also up-regulated in RCC cells. Interestingly, knockdown of either of TMEM22 or RAB37 expression by specific siRNA caused significant reduction of cancer cell growth. Our results imply that the TMEM22/RAB37 complex is likely to play a crucial role in growth of RCC and that inhibition of the TMEM22/RAB37 expression or their interaction should be novel therapeutic targets for RCC.
authors.affiliation: Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Dobashi
authors.forename: Toyomasa
authors.initials: T
authors.lastname: Katagiri
authors.forename: Eiji
authors.initials: E
authors.lastname: Hirota
authors.forename: Singo
authors.initials: S
authors.lastname: Ashida
authors.forename: Yataro
authors.initials: Y
authors.lastname: Daigo
authors.forename: Taro
authors.initials: T
authors.lastname: Shuin
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Fujioka
authors.forename: Tsuneharu
authors.initials: T
authors.lastname: Miki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D020691:rab GTP-Binding Proteins