title: Effects of arsenic (As) exposure on the antioxidant status of gills of the zebrafish Danio rerio (Cyprinidae).
abstract: In fishes, arsenic (As) is absorbed via the gills and is capable of causing disturbance to the antioxidant system. The objective of present study was to evaluate antioxidant responses after As exposure in gills of zebrafish (Danio rerio, Cyprinidae). Fish were exposed for 48 h to three concentration of As, including the highest As concentration allowed by current Brazilian legislation (10 microg As/L). A control group was exposed to tap water (pH 8.0; 26 degrees C; 7.20 mg O(2)/L). As exposure resulted in (1) an increase (p<0.05) of glutathione (GSH) levels after exposure to 10 and 100 microg As/L, (2) an increase of the glutamate cysteine ligase (GCL) activity in the same concentrations (p<0.05), (3) no significant differences in terms of glutathione reductase, glutathione-S-transferase and catalase activities; (4) a significantly lower (p<0.05) oxygen consumption after exposure to 100 microg As/L; (4) no differences in terms of oxygen reactive species generation and lipid peroxidation content (p>0,05). In the gills, only inorganic As was detected. Overall, it can be concluded that As affected the antioxidant responses increasing GCL activity and GSH levels, even at concentration considered safe by Brazilian legislation.
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil.
authors.forename: Juliane
authors.initials: J
authors.lastname: Ventura-Lima
authors.forename: Micheli Rosa
authors.initials: MR
authors.lastname: de Castro
authors.forename: Daiane
authors.initials: D
authors.lastname: Acosta
authors.forename: Daniele
authors.initials: D
authors.lastname: Fattorini
authors.forename: Francesco
authors.initials: F
authors.lastname: Regoli
authors.forename: Leandro Machado
authors.initials: LM
authors.lastname: de Carvalho
authors.forename: Denise
authors.initials: D
authors.lastname: Bohrer
authors.forename: Laura A
authors.initials: LA
authors.lastname: Geracitano
authors.forename: Daniela Martí
authors.initials: DM
authors.lastname: Barros
authors.forename: Luis F F
authors.initials: LF
authors.lastname: Marins
authors.forename: Rosane Souza
authors.initials: RS
authors.lastname: da Silva
authors.forename: Carla Denise
authors.initials: CD
authors.lastname: Bonan
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
authors.forename: José M
authors.initials: JM
authors.lastname: Monserrat
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D005880:Gills / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D010101:Oxygen Consumption / Q000187:drug effects; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D001151:Arsenic