title: Expression of four housekeeping proteins in elderly patients with schizophrenia.
abstract: We compared protein expression by Western blot analysis in four areas of postmortem brain from patients with schizophrenia and control subjects for several proteins that are often used as controls for Western blot studies: beta-tubulin, actin, glyceraldehyde-3-phosphate dehydrogenase, and valosin-containing protein. We did not detect any differences in expression between subjects with schizophrenia and a comparison group. These results suggest that all four proteins are suitable loading controls for postmortem studies of schizophrenia.
authors.affiliation: Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, SC560, 1530 3rd Ave S., Birmingham, AL, 35294-0017, USA. debbauer@uab.edu
authors.forename: Deborah Elaine
authors.initials: DE
authors.lastname: Bauer
authors.forename: Vahram
authors.initials: V
authors.lastname: Haroutunian
authors.forename: Robert E
authors.initials: RE
authors.lastname: McCullumsmith
authors.forename: James H
authors.initials: JH
authors.lastname: Meador-Woodruff
mesh_terms: D000199:Actins / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000368:Aged; D000704:Analysis of Variance; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006179:Gyrus Cinguli / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D017397:Prefrontal Cortex / Q000378:metabolism; D012559:Schizophrenia / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D000074405:Valosin Containing Protein; D014793:Visual Cortex / Q000378:metabolism
chemical_list: D000199:Actins; D018797:Cell Cycle Proteins; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000251:Adenosine Triphosphatases; C000614178:VCP protein, human; D000074405:Valosin Containing Protein