Retroviral gene transduction into chicken embryo gonads through blood circulation.
A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.
Kawabe
Yoshinori
Y
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Naka
Tsutomu
T
Komatsu
Hiroyuki
H
Nishijima
Ken-ichi
K
Iijima
Shinji
S
Kamihira
Masamichi
M
D000818:Animals; D001483:Base Sequence; D001775:Blood Circulation; D002642:Chick Embryo; D017931:DNA Primers; D005822:Genetic Vectors; D005854:Germ Cells / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic; D019076:Transgenes
D017931:DNA Primers