title: Pomegranin, an antifungal peptide from pomegranate peels.
abstract: A new antifungal peptide designated as pomegranin, with an N-terminal sequence resembling that of rice disease resistance NB-S-LRR-like protein, was isolated from fresh pomegranate peels by ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Pomegranin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. It exhibited a molecular mass of 11 kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It inhibited mycelial growth in the fungi Botrytis cinerea and Fusarium oxysporum with an IC(50) of 2 microM and 6.1 microM, respectively. It was devoid of hemagglutinating, ribonuclease, deoxyribonuclease and protease inhibitory activities.
authors.affiliation: State Key Laboratory for Agrobiotechnology and College of Biological Science, China Agricultural University, Beijing, 100094, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Guo
authors.forename: He Xiang
authors.initials: HX
authors.lastname: Wang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D020171:Botrytis / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005659:Fungicides, Industrial / Q000302:isolation & purification / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects; D029561:Lythraceae / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D005659:Fungicides, Industrial; D010940:Plant Proteins; C538803:pomegranin protein, Punica granatum