Identification of the expression profile of apoptotic esophageal cancer cells by adenoviral-fragile histidine triad treatment.
BACKGROUND
The fragile histidine triad (FHIT) functions as a tumor suppressor, and giving adenoviral-FHIT (Ad-FHIT) is thus expected to be clinically beneficial. Much attention has recently been focused on which genes are commonly regulated by Ad-FHIT, and which genes are dominant in Ad-FHIT-induced apoptotic cells.


METHODS
Ad-FHIT apoptosis-induced cells (H1299 and TE4) and non-apoptosis-induced cells (TE2) were used in the current experiments. The total RNA extracted from Ad-FHIT or control was labeled with Cy3-dCTP or Cy5-dCTP and hybridized with 19,192 genes on a chip. A microarray analysis for each gene was carried out with high reproducibility provided by seven independent experiments and duplicated oligos on a chip.


RESULTS
We listed the upregulated genes based on the TE4:TE2 expression ratio, such as c-Src, Jak-1, and sialyltransferase, which are expected to be target pathways as well as the downregulated genes, including CASP8 and CASP10, after Ad-FHIT treatment in esophageal cancer.


CONCLUSIONS
The current microarray analysis indicated that the apoptosis of esophageal cancer observed after giving Ad-FHIT was possibly induced by activation of the c-Src gene and inactivation of the CASP8 gene.
Mimori
Koshi
K
Department of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan.
Ishii
Hideshi
H
Inoue
Hiroshi
H
Barnard
Graham F
GF
Mori
Masaki
M
D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics*; D053181:Caspase 8 / Q000235:genetics; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D016391:Genes, src; D005822:Genetic Vectors; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D015533:Transcriptional Activation; D014161:Transduction, Genetic; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D053181:Caspase 8; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C505530:CASP8 protein, human