Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source.
We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
Blandin
Pierre
P
Laboratoire de Photophysique Moléculaire, CNRS, Université Paris-Sud, Bat 210, 91405 Orsay, France.
Lévêque-Fort
Sandrine
S
Lécart
Sandrine
S
Cossec
Jack C
JC
Potier
Marie-Claude
MC
Lenkei
Zsolt
Z
Druon
Frédéric
F
Georges
Patrick
P
D002460:Cell Line; D004867:Equipment Design; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D009446:Neurobiology / Q000379:methods; D055095:Optics and Photonics; D025941:Protein Interaction Mapping; D012031:Refractometry / Q000379:methods; D015203:Reproducibility of Results; D013997:Time Factors
C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins