title: Ecological significance and some biotechnological application of an organic solvent stable alkaline serine protease from Bacillus subtilis strain DM-04.
abstract: An organic solvent stable, alkaline serine protease (Bsubap-I) with molecular mass of 33.1 kDa, purified from Bacillus subtilis DM-04 showed optimum activity at temperature and pH range of 37-45 degrees C and 10.0-10.5, respectively. The enzyme activity of Bsubap-I was significantly enhanced in presence of Fe(2+). The thermal resistance and stability and of Bsubap-I in presence of surfactants, detergents, and organic solvents, and its dehairing activity supported its candidature for application in laundry detergent formulations, ultrafiltration membrane cleaning, peptide synthesis and in leather industry. The broad substrate specificity and differential antibacterial property of Bsubap-I suggested the natural ecological role of this enzyme for the producing bacterium.
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784 028, Assam, India.
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Rai
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Mukherjee
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001709:Biotechnology; D003902:Detergents / Q000737:chemistry; D004795:Enzyme Stability; D006204:Hair Removal; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D009930:Organic Chemicals / Q000737:chemistry*; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003902:Detergents; D009930:Organic Chemicals; D007501:Iron; D012697:Serine Endopeptidases