Integration host factor (IHF) dictates the structure of polyamine-DNA condensates: implications for the role of IHF in the compaction of bacterial chromatin.
Integration host factor (IHF), a nucleoid-associated protein in bacterial cells, is implicated in a number of chromosomal functions including DNA compaction. IHF binds to all duplex DNA with micromolar affinity and at sequence-specific sites with much higher affinity. IHF is known to induce sharp bends in the helical axis of DNA in both modes of binding, but the role of IHF in controlling DNA condensation within bacterial cells has remained undetermined. Here we demonstrate that IHF influences the morphology of DNA condensed by polyamines in vitro. In the absence of IHF, spermidine and spermine condense DNA primarily into toroidal structures, whereas in the presence of IHF, polyamines condense DNA primarily into rodlike structures. Computer simulations of DNA condensation in the absence and presence of IHF binding lend support to our model in which DNA bending proteins, such as IHF and HU, promote the condensation of DNA into rodlike structures by providing the free energy necessary to bend DNA at the ends of linear bundles of condensed DNA. We propose that a common function of IHF and HU in bacterial cells is to facilitate DNA organization in the nucleoid by the introduction of sharp bends in chromosomal DNA.
Sarkar
Tumpa
T
School of Chemistry and Biochemistry, Petit Institute of Bioengineering and Bioscience, and School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Petrov
Anton S
AS
Vitko
Jason R
JR
Santai
Catherine T
CT
Harvey
Stephen C
SC
Mukerji
Ishita
I
Hud
Nicholas V
NV
D001426:Bacterial Proteins / Q000502:physiology*; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D006657:Histones / Q000737:chemistry / Q000502:physiology; D034982:Integration Host Factors / Q000737:chemistry / Q000502:physiology*; D011073:Polyamines / Q000737:chemistry*
D001426:Bacterial Proteins; D002843:Chromatin; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006657:Histones; D034982:Integration Host Factors; D011073:Polyamines; C116822:hns protein, E coli