ben Functions with scamp during synaptic transmission and long-term memory formation in Drosophila.
Genetic screens for Drosophila mutants defective in pavlovian olfactory memory have provided unique insight into the molecular basis of memory storage. Occasionally, these singular genetic lesions have been assembled into meaningful molecular pathways and neural circuitries. For the most part, however, these genes and their expression patterns in the CNS remain fragmented, demanding new clues from continued mutant screens. From a behavioral screen for long-term memory (LTM) mutants, we have identified ben (CG32594), which encodes a novel protein. Mutations of ben specifically disrupt LTM, leaving earlier memory phases intact. The role of ben appears physiological rather than developmental, because acutely induced expression of a ben(+) transgene in adults rescues the mutant's LTM defect. More interestingly, induced expression of ben(+) specifically in mushroom bodies (MBs), but not in the ellipsoid body of the central complex, is sufficient to rescue the mutant LTM defect. This suggests a role for ben in the MB during olfactory memory formation. We also provide evidence that BEN interacts genetically in both synaptic transmission and LTM formation with SCAMP, a synaptic protein known to be involved in vesicle recycling.
Zhao
Hong
H
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China.
Zheng
Xingguo
X
Yuan
Xiaojing
X
Wang
Lei
L
Wang
Xin
X
Zhong
Yi
Y
Xie
Zuoping
Z
Tully
Tim
T
D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D003214:Conditioning, Classical / Q000502:physiology; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics; D007814:Larva; D008568:Memory / Q000502:physiology*; D008569:Memory Disorders / Q000235:genetics*; D009043:Motor Activity / Q000235:genetics; D024521:Mushroom Bodies / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009469:Neuromuscular Junction / Q000235:genetics; D009833:Olfactory Pathways / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics*; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000502:physiology*
D002352:Carrier Proteins; D029721:Drosophila Proteins; D012333:RNA, Messenger; D044763:Ubiquitin-Conjugating Enzymes; C471544:ben protein, Drosophila