title: Bioorthogonal ligation in the spotlight.
abstract: The light-induced formation of a covalent bond between an alkene group and a tetrazole moiety has been used for the selective labeling of proteins in vitro and in live cells. This bioorthogonal ligation initiated by brief irradiation with UV light leads to a fluorescent pyrazoline adduct (see scheme).
authors.affiliation: Fakultät Chemie/Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kurpiers
authors.forename: Henning D
authors.initials: HD
authors.lastname: Mootz
mesh_terms: D000475:Alkenes / Q000737:chemistry; D003500:Cyclization; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000528:radiation effects; D055668:Photochemical Processes; D011506:Proteins / Q000737:chemistry*; D013777:Tetrazoles / Q000737:chemistry*; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D000475:Alkenes; D005456:Fluorescent Dyes; D011506:Proteins; D013777:Tetrazoles