Identification of promising antigenic components in latent fingermark residues.
An analysis of latent fingermark residues by Sodium-Dodecyl-Sulfate PolyAcrylamide Gel Electrophoresis (SDS-PAGE) followed by silver staining allowed the detection of different proteins, from which two major bands, corresponding to proteins of 56 and 64 kDa molecular weight, could be identified. Two other bands, corresponding to proteins of 52 and 48 kDa were also visualizable along with some other weaker bands of lower molecular weights. In order to identify these proteins, three antibodies directed against human proteins were tested on western blots of fingermarks residues: anti-keratin 1 and 10 (K1/10), anti-cathepsin-D (Cat.D) and anti-dermcidin (Derm.). The corresponding antigens are known to be present in the stratum corneum of desquamating stratified epithelium (K1/10, Cat.D) and/or in eccrine sweat (Cat.D, Derm.). The two major bands were identified as consistent with keratin 1 and 10. The pro-form and the active form of the cathepsin-D have also been identified from two other bands. Dermcidin could not be detected in the western blot. In addition, these antibodies have been tested on latent fingermarks left on polyvinylidene fluoride (PVDF) membrane, as well as on whitened and non-whitened paper. The detection of fingermarks was successful with all three antibodies.
Drapel
Valérie
V
University of Lausanne-ESC, Lausanne, Switzerland. valerie.drapel@unil.ch
Becue
Andy
A
Champod
Christophe
C
Margot
Pierre
P
D000906:Antibodies; D015153:Blotting, Western; D002402:Cathepsin D / Q000032:analysis* / Q000276:immunology; D005591:Chemical Fractionation / Q000379:methods; D003878:Dermatoglyphics; D004439:Eccrine Glands; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D053556:Keratin-1 / Q000032:analysis* / Q000276:immunology; D053550:Keratin-10 / Q000032:analysis* / Q000276:immunology; D008297:Male; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D012627:Sebaceous Glands; D013499:Surface Properties; D013542:Sweat / Q000737:chemistry
D000906:Antibodies; D053556:Keratin-1; D010455:Peptides; C442243:dermcidin; D053550:Keratin-10; D002402:Cathepsin D