title: Centrifugal partition chromatography directly interfaced with mass spectrometry for the fast screening and fractionation of major xanthones in Garcina mangostana.
abstract: Xanthones are well known for their interesting phytochemical properties, which make them attractive to the pharmaceutical and medicinal industry. We have therefore developed a method to analyse the major xanthones in Garcina mangostana. The xanthones were extracted by pressurized liquid extraction with ethanol and separated at the semi-preparative scale by centrifugal partition chromatography (CPC) with a biphasic solvent system consisting of heptane/ethyl acetate/methanol/water (2:1:2:1, v/v/v/v). A CPC-electrospray ionisation MS coupling was performed and used to simultaneously separate and identify the compounds. Thanks to a variable flow splitter and an additional stream of ethanol/1 mol L(-1) ammonium acetate (95:5, v/v), all the compounds were ionised, detected and monitored whatever the solvents used in mobile phase for the CPC separation. The dual mode or elution-extrusion which are less solvent-consuming and faster than the elution mode were used without loss of ionisation and detection.
authors.affiliation: ICOA, Université d'Orléans, UMR CNRS 6005, 45067 Orléans Cedex 2, France. emilie.destandau@univ-orleans.fr
authors.forename: E
authors.initials: E
authors.lastname: Destandau
authors.forename: A
authors.initials: A
authors.lastname: Toribio
authors.forename: M
authors.initials: M
authors.lastname: Lafosse
authors.forename: V
authors.initials: V
authors.lastname: Pecher
authors.forename: C
authors.initials: C
authors.lastname: Lamy
authors.forename: P
authors.initials: P
authors.lastname: André
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D029762:Garcinia mangostana / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D012680:Sensitivity and Specificity; D044004:Xanthones / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D044004:Xanthones