title: Trypanosoma cruzi bromodomain factor 2 (BDF2) binds to acetylated histones and is accumulated after UV irradiation.
abstract: Histone tail post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination and ADP-ribosylation) regulate many cellular processes. Among these modifications, phosphorylation, methylation and acetylation have already been described in trypanosomatid histones. Bromodomains, together with chromodomains and histone-binding SANT domains, were proposed to be responsible for "histone code" reading. The Trypanosoma cruzi genome encodes four coding sequences (CDSs) that contain a bromodomain, named TcBDF1-4. Here we show that one of those, TcBDF2, is expressed in discrete regions inside the nucleus of all the parasite life cycle stages and binds H4 and H2A purified histones from T. cruzi. Immunolocalization experiments using both anti-histone H4 acetylated peptides and anti-TcBDF2 antibodies determined that TcBDF2 co-localizes with histone H4 acetylated at lysines K10 and K14. TcDBF2 and K10 acetylated H4 interaction was confirmed by co-immunoprecipitation. It is also shown that TcBDF2 was accumulated after UV irradiation of T. cruzi epimastigotes. These results suggest that TcBDF2 could be taking part in a chromatin remodelling complex in T. cruzi.
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Suipacha 351, 2000, Rosario, Argentina.
authors.forename: Gabriela Vanina
authors.initials: GV
authors.lastname: Villanova
authors.forename: Sheila Cristina
authors.initials: SC
authors.lastname: Nardelli
authors.forename: Pamela
authors.initials: P
authors.lastname: Cribb
authors.forename: Anahí
authors.initials: A
authors.lastname: Magdaleno
authors.forename: Ariel Mariano
authors.initials: AM
authors.lastname: Silber
authors.forename: Maria Cristina M
authors.initials: MC
authors.lastname: Motta
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.forename: Esteban
authors.initials: E
authors.lastname: Serra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016000:Cluster Analysis; D006657:Histones / Q000378:metabolism*; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D016415:Sequence Alignment; D014349:Trypanosoma cruzi / Q000502:physiology* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D006657:Histones; D015800:Protozoan Proteins