[Roles of matrix metalloproteinases and tissue specific inhibitors of metalloproteinases in dentinogenic ghost cell tumor and ghost cell odontogenic carcinoma].
OBJECTIVE
To investigate the roles of matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in dentinogenic ghost cell tumor (DGCT) and ghost cell odontogenic carcinoma (GCOC).


METHODS
The expressions of MMP-2, MMP-9, MMP-14, TIMP-1 and TIMP-2 were examined in 15 DGCT cases and 9 GCOC cases by immunohistochemistry. Their mRNA expression in one DGCT case and one GCOC case were investigated by RT-PCT.MMP-2 and MMP-9 protein activities in the two cases were analyzed by gelatin zymography.


RESULTS
MMP-9 and TIMP-1 expressions elevated greatly in GCOC, and there was a significant difference (P < 0.05) in TIMP-1 expression between GCOC and DGCT.Pro-MMP-9, MMP-9 activated form, pro-MMP-2, and MMP-2 activated forms were detected in the GCOC case, while pro-MMP-9 and MMP-9 activated form were very faint in the DGCT case. The mRNA level of MMP-9 elevated obviously in the GCOC case, which was similar to that of TIMP-1.


CONCLUSIONS
The elevated expression of MMP-9 and TIMP-1 may influence the behaviour of GCOC.
Geng
Ning
N
Department of Oral Pathology, West China College of Stomatology, Sichuan University, Chengdu, China.
Ban
Yu
Y
Chen
Yu
Y
Yang
Ming-Zhong
MZ
Bao
Dong-Mei
DM
Yi
Xin-Zhu
XZ
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003804:Dentin; D006801:Humans; D008339:Mandibular Neoplasms / Q000378:metabolism* / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D055815:Young Adult
D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases