title: Genetic analysis and pathogenicity of betanodavirus isolated from wild redspotted grouper Epinephelus akaara with clinical signs.
abstract: Diseased wild redspotted grouper Epinephelus akaara were collected from Seto Inland Sea, Ehime Prefecture, in August 2002. Fish showed erratic swimming behavior and inflation of the swim bladder. The fish brains were positive for nodavirus in both RT-PCR and nested PCR. The sequence of the nested PCR product (177 nt) was closely related to that of a known betanodavirus, redspotted grouper nervous necrosis virus. When juvenile sevenband grouper E. septemfasciatus were challenged intravitreously with virus, abnormal swimming behavior and high mortality were observed. This is the first report on viral nervous necrosis in a wild population of redspotted grouper with clinical signs.
authors.affiliation: Brain Korea 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Gomez
authors.forename: Satoru
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Koh-ichiro
authors.initials: K
authors.lastname: Mori
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okinaka
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakai
mesh_terms: D000818:Animals; D001492:Bass / Q000821:virology*; D001921:Brain / Q000821:virology; D036022:Capsid Proteins / Q000145:classification / Q000235:genetics*; D005393:Fish Diseases / Q000821:virology*; D005814:Genes, Viral; D007564:Japan; D030381:Nodaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D014774:Virulence / Q000235:genetics
chemical_list: D036022:Capsid Proteins