title: Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice.
abstract: Rice stripe virus (RSV) infects rice and causes great yield reduction in some Asian countries. In this study, rice cDNA library was screened by a Gal4-based yeast two-hybrid system using pc4, a putative movement protein of RSV, as the bait. A number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. Two of them were selected and further characterized. The two proteins were a J protein and a small Hsp, respectively. Interactions between Pc4 and the two proteins were confirmed using coimmunoprecipitation. Implications of the findings that pc4 interacted with two chaperone proteins were discussed.
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Lianming
authors.initials: L
authors.lastname: Lu
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.forename: Meiling
authors.initials: M
authors.lastname: Qin
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Hanhong
authors.initials: H
authors.lastname: Lan
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Niu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Jia
authors.forename: Liyan
authors.initials: L
authors.lastname: Xie
authors.forename: Qiying
authors.initials: Q
authors.lastname: Lin
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism*; D050888:Heat-Shock Proteins, Small / Q000378:metabolism; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D010940:Plant Proteins / Q000378:metabolism*; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016415:Sequence Alignment; D029364:Tenuivirus / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D050956:HSP40 Heat-Shock Proteins; D050888:Heat-Shock Proteins, Small; D010940:Plant Proteins; D053830:Plant Viral Movement Proteins