Distribution of HLA-B27 and its alleles in patients with reactive arthritis and with ankylosing spondylitis in Tunisia.
The purpose of the present study is to investigate the frequency of HLA-B27 and its alleles in reactive arthritis (ReA) and in ankylosing spondylitis (AS) in Tunisia. HLA-B27 alleles were typed by PCR amplification with sequence-specific primers. We studied 17 patients with ReA associated with urethritis or with gastrointestinal infection; 42 HLA-B27-positive patients with AS and 100 healthy controls. Eleven ReA patients (67.7%) were HLA-B27 positive. There was an increased frequencies of HLA-B27 (P = 7.76 x 10(-12), OR = 59.30) and a moderate increase of HLA-B51 (P = 0.015; OR = 4.91) alleles in ReA patients when compared with healthy controls. Four B27 subtypes were identified: B*2702, 05, 09 and B*2712. The distribution of these alleles in the ReA patients was 37.5% for B*2702 and B*2705. Only these two subtypes were detected in 18 (42.8%) and 24 (57.1%), respectively, of the AS patients. B*2709 and B*2712 were relatively rare in ReA patients and were identified in one case each. Our results showed a restricted number of HLA-B27 subtypes associated with ReA and AS. B*2702 and 2705 were common in ReA and AS patients.
Siala
Mariam
M
Laboratoire de Recherche Micro-organismes et Pathologie Humaine, EPS Habib BOURGUIBA de Sfax, Sfax, Tunisia. mariamsiela@voila.fr
Mahfoudh
Nadia
N
Gdoura
Radhouane
R
Younes
Mohamed
M
Fourati
Hela
H
Kammoun
Arwa
A
Chour
Ilhem
I
Meddeb
Nihel
N
Gaddour
Lilia
L
Hakim
Faiza
F
Baklouti
Sofien
S
Bargaoui
Naceur
N
Sellami
Sleheddine
S
Hammami
Adnene
A
Makni
Hafedh
H
D000328:Adult; D000483:Alleles; D016918:Arthritis, Reactive / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005787:Gene Frequency; D015796:HLA-B27 Antigen / Q000235:genetics* / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D000091402:Prohibitins; D013167:Spondylitis, Ankylosing / Q000235:genetics* / Q000276:immunology; D014416:Tunisia
D015796:HLA-B27 Antigen; C000717331:PHB2 protein, human; D000091402:Prohibitins