title: Hormographiella aspergillata keratomycosis in a dog.
abstract: A 4-year-old, female, Border Collie was presented to the University of Bern Veterinary Teaching Hospital, because of a corneal lesion of 10 days duration. The axial cornea presented a whitish fluorescein-positive plaque with irregular margins. A diagnosis of keratomycosis was made based on cytology. Medical therapy with local broad-spectrum antibiotic and fluconazole was instituted. After 1 week of treatment, the improvement was deemed unsatisfactory. Therefore, a lamellar keratectomy and conjunctival pedicle flap were performed. After surgery, the cornea healed uneventfully. Histology confirmed the diagnosis of keratomycosis. The fungus could not be grown in culture and a precise etiological diagnosis could only be obtained with genetic identification of the fungus. A PCR technique was used to amplify the fungal genome from the cornea. Hormographiella aspergillata, the asexual reproductive form of the basidiomycete Coprinopsis cinerea, was identified. As advised in human medicine, we encourage the use of this molecular technique to obtain an early species diagnosis, allowing targeted medical therapy.
authors.affiliation: Department of Clinical Veterinary Medicine, Ophthalmology Service, Vetsuisse Faculty, University of Bern, Bern, Switzerland. antonella.rampazzo@itn.unibe.ch
authors.forename: Antonella
authors.initials: A
authors.lastname: Rampazzo
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.forename: Judith
authors.initials: J
authors.lastname: Howard
authors.forename: Valérie
authors.initials: V
authors.lastname: Bornand
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D015169:Colony Count, Microbial / Q000662:veterinary; D003131:Combined Modality Therapy; D003302:Coprinus / Q000187:drug effects / Q000302:isolation & purification*; D003315:Cornea / Q000382:microbiology / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000601:surgery; D004285:Dogs; D015821:Eye Infections, Fungal / Q000175:diagnosis / Q000188:drug therapy / Q000601:surgery / Q000662:veterinary*; D005260:Female; D015725:Fluconazole / Q000627:therapeutic use; D007634:Keratitis / Q000175:diagnosis / Q000188:drug therapy / Q000601:surgery / Q000662:veterinary*; D009181:Mycoses / Q000175:diagnosis / Q000188:drug therapy / Q000601:surgery / Q000662:veterinary*; D016896:Treatment Outcome
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole