Anti-infectious activity of tryptophan metabolites in the L-tryptophan-L-kynurenine pathway.
To study the anti-infectious effect of a vascular allograft, the antimicrobial activity of tryptophan metabolites mediated by indoleamine 2,3-dioxygenase was determined. The growth of methicillin-resistant Staphylococcus aureus (MRSA) over 10 h in extracts from post-transplantation vascular allograft was significantly slower than that of extracts from non-transplantation vascular allograft regardless of the presence of tryptophan (p<0.05). When the antimicrobial activity of the tryptophan metabolites in the L-tryptophan-L-kynurenine pathway was examined, 3-hydroxy-DL-kynurenine and alpha-picolinic acid had strong antibacterial activity against MRSA, S. epidermidis, Escherichia coli, and multidrug-resistant Pseudomonas aeruginosa, although antimicrobial activities of anthranilic acid, 3-hydroxyanthranilic acid, and quinolinic acid against them were low. The results showed that, of the tested tryptophan metabolites, 3-hydroxy-DL-kynurenine and alpha-picolinic acid contributed to the anti-infectious effects of the allograft by inhibiting of the growth of microorganisms.
Narui
Koji
K
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan.
Noguchi
Norihisa
N
Saito
Aya
A
Kakimi
Kazuhiro
K
Motomura
Noboru
N
Kubo
Kinya
K
Takamoto
Shinichi
S
Sasatsu
Masanori
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D007502:Iron Chelating Agents / Q000494:pharmacology; D007737:Kynurenine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism*; D010848:Picolinic Acids / Q000494:pharmacology; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007502:Iron Chelating Agents; D010848:Picolinic Acids; C005045:3-hydroxykynurenine; D007737:Kynurenine; D014364:Tryptophan; C030614:picolinic acid