Identification of psychrophile Shewanella sp. KMG427 as an eicosapentaenoic acid producer.
An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4o degrees C after cell proliferation at 20 degrees C. The isolated bacterium was categorized as Gramnegative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.
Lee
Won-Hae
WH
Department of Microbiology, Kyungpook National University, Daegu 702-701, Korea.
Cho
Ki-Woong
KW
Park
Soo-Young
SY
Shin
Kee-Sun
KS
Lee
Dong-Sun
DS
Hwang
Seon-Kap
SK
Seo
Seok-Jong
SJ
Kim
Jong-Myeong
JM
Ghim
Sa-Youl
SY
Song
Bang-Ho
BH
Lee
Sang-Han
SH
Kim
Jong-Guk
JG
D002849:Chromatography, Gas; D004275:DNA, Ribosomal / Q000235:genetics; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis; D005798:Genes, Bacterial; D008563:Membrane Lipids / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020592:Shewanella / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D013696:Temperature
D004275:DNA, Ribosomal; D005227:Fatty Acids; D008563:Membrane Lipids; D012336:RNA, Ribosomal, 16S; D015118:Eicosapentaenoic Acid