title: Diminished acute phase response and increased hepatic inflammation of aged rats in response to intraperitoneal injection of lipopolysaccharide.
abstract: Aging is associated with a deterioration of the acute phase response to inflammatory challenges. However, the nature of these defects remains poorly defined. We analyzed the hepatic inflammatory response after intraperitoneal administration of lipopolysaccharide (LPS) given to Fisher 344 rats aged 6, 15, and 22-23 months. Induction of the acute phase proteins (APPs), haptoglobin, alpha-1-acid glycoprotein, and T-kininogen was reduced and/or retarded with aging. Initial induction of interleukin-6 in aged rats was normal, but the later response was increased relative to younger counterparts. An exacerbated hepatic injury was observed in aged rats receiving LPS, as evidenced by the presence of multiple microabscesses in portal tracts, confluent necrosis, higher neutrophil accumulation, and elevated serum levels of alanine aminotransferase, relative to younger animals. Our results suggest that aged rats displayed a reduced expression of APPs and increased hepatic injury in response to the inflammatory insult.
authors.affiliation: National Institute on Aging, NIA/NIH, Bethesda MD 20892, USA.
authors.forename: Christian R
authors.initials: CR
authors.lastname: Gomez
authors.forename: Claudio
authors.initials: C
authors.lastname: Acuña-Castillo
authors.forename: Claudio
authors.initials: C
authors.lastname: Pérez
authors.forename: Elías
authors.initials: E
authors.lastname: Leiva-Salcedo
authors.forename: Denise M
authors.initials: DM
authors.lastname: Riquelme
authors.forename: Gamaliel
authors.initials: G
authors.lastname: Ordenes
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Oshima
authors.forename: Mauricio
authors.initials: M
authors.lastname: Aravena
authors.forename: Viviana I
authors.initials: VI
authors.lastname: Pérez
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Nishimura
authors.forename: Valeria
authors.initials: V
authors.lastname: Sabaj
authors.forename: Robin
authors.initials: R
authors.lastname: Walter
authors.forename: Felipe
authors.initials: F
authors.lastname: Sierra
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000375:Aging / Q000276:immunology*; D000818:Animals; D006520:Hepatitis, Animal / Q000276:immunology*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000009:adverse effects; D008099:Liver / Q000378:metabolism; D008297:Male; D009504:Neutrophils; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000209:Acute-Phase Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides