title: Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors.
abstract: The preparation, characterization and application of a three-layer magnetic nanoparticle composed of an Fe(3)O(4) magnetic core, a Prussian Blue (PB) interlayer and a gold shell (it can be abbreviated as Au-PB-Fe(3)O(4)) for an ultrasensitive and reproducible electrochemical immunosensing fabrication were described for the first time in this work. With the employment of the Au-PB-Fe(3)O(4) nanoparticle, a new signal amplification strategy was developed based on bienzyme (horseradish peroxidase and glucose oxidase) functionalized Au-PB-Fe(3)O(4) nanoparticles for an electrochemical immunosensing fabrication by using Carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as model systems, respectively. The experiment results show that the multilabeled Au-PB-Fe(3)O(4) nanoparticles exhibit satisfying redox electrochemical activity and high enzyme catalysis activity, which predetermine their utility in high sensitivity antibody detection schemes. Furthermore, this immunosensor could be regenerated by simply using an external magnetic field which ensured a reproducible immunosensor with high sensitivity.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Pei-Xi
authors.initials: PX
authors.lastname: Yuan
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Chai
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Hong
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D055664:Electrochemical Techniques / Q000379:methods*; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D007501:Iron / Q000737:chemistry; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission
chemical_list: D004798:Enzymes; D007501:Iron