title: In vitro characterization of HCN channel kinetics and frequency dependence in myocytes predicts biological pacemaker functionality.
abstract: The pacemaker current, mediated by hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, contributes to the initiation and regulation of cardiac rhythm. Previous experiments creating HCN-based biological pacemakers in vivo found that an engineered HCN2/HCN1 chimeric channel (HCN212) resulted in significantly faster rates than HCN2, interrupted by 1-5 s pauses. To elucidate the mechanisms underlying the differences in HCN212 and HCN2 in vivo functionality as biological pacemakers, we studied newborn rat ventricular myocytes over-expressing either HCN2 or HCN212 channels. The HCN2- and HCN212-over-expressing myocytes manifest similar voltage dependence, current density and sensitivity to saturating cAMP concentrations, but HCN212 has faster activation/deactivation kinetics. Compared with HCN2, myocytes expressing HCN212 exhibit a faster spontaneous rate and greater incidence of irregular rhythms (i.e. periods of rapid spontaneous rate followed by pauses). To explore these rhythm differences further, we imposed consecutive pacing and found that activation kinetics of the two channels are slower at faster pacing frequencies. As a result, time-dependent HCN current flowing during diastole decreases for both constructs during a train of stimuli at a rapid frequency, with the effect more pronounced for HCN2. In addition, the slower deactivation kinetics of HCN2 contributes to more pronounced instantaneous current at a slower frequency. As a result of the frequency dependence of both instantaneous and time-dependent current, HCN2 exhibits more robust negative feedback than HCN212, contributing to the maintenance of a stable pacing rhythm. These results illustrate the benefit of screening HCN constructs in spontaneously active myocyte cultures and may provide the basis for future optimization of HCN-based biological pacemakers.
authors.affiliation: Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, NY, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Annalisa
authors.initials: A
authors.lastname: Bucchi
authors.forename: Ronit V
authors.initials: RV
authors.lastname: Oren
authors.forename: Yelena
authors.initials: Y
authors.lastname: Kryukova
authors.forename: Wen
authors.initials: W
authors.lastname: Dun
authors.forename: Colleen E
authors.initials: CE
authors.lastname: Clancy
authors.forename: Richard B
authors.initials: RB
authors.lastname: Robinson
mesh_terms: D000200:Action Potentials; D000818:Animals; D000831:Animals, Newborn; D001683:Biological Clocks; D002304:Cardiac Pacing, Artificial; D002478:Cells, Cultured; D003198:Computer Simulation; D000242:Cyclic AMP / Q000378:metabolism; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000235:genetics / Q000378:metabolism*; D006339:Heart Rate; D006352:Heart Ventricles / Q000378:metabolism; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008955:Models, Cardiovascular; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014161:Transduction, Genetic
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; C578667:Hcn1 protein, rat; C578712:Hcn2 protein, rat; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D015221:Potassium Channels; D000242:Cyclic AMP; D011188:Potassium