title: Evaluation of genetic variation in the clown Knifefish, Chitala chitala, using allozymes, RAPD, and microsatellites.
abstract: Twenty-seven enzyme systems, six random amplified polymorphic DNA (RAPD) primers, and two microsatellite loci were tested to determine intraspecific divergence in the natural population of the endangered Indian featherback fish, Chitala chitala, for the first time. The 262 samples of C. chitala were collected from six riverine locations in India: the Satluj, Ganga (Ghagra, Bhagirathi, and Brahmaputra), Mahanadi, and Narmada river systems. The analysis revealed population subdivisions, with an F(ST) value from 0.1235 (95% confidence 0.0868-0.1621) for RAPD and a combined F(ST) of 0.0344 (95% confidence 0.0340-0.0350) for microsatellite loci. An analysis of 38 allozyme loci did not reveal any polymorphism in the samples from any of the riverine localities; a possible explanation for this could be that the ancestors of Chitala could have faced a population reduction in prehistoric periods, as low allozyme variation is also reported for other species of Chitala from south Asia.
authors.affiliation: National Bureau of Fish Genetic Resources (ICAR), Canal Ring Road, P.O. Dilkusha, Lucknow, 226002, UP, India.
authors.forename: Anup
authors.initials: A
authors.lastname: Mandal
authors.forename: Kuldeep Kumar
authors.initials: KK
authors.lastname: Lal
authors.forename: Vindhya
authors.initials: V
authors.lastname: Mohindra
authors.forename: Rajeev Kumar
authors.initials: RK
authors.lastname: Singh
authors.forename: Peyush
authors.initials: P
authors.lastname: Punia
authors.forename: U K
authors.initials: UK
authors.lastname: Chauhan
authors.forename: Wazir Singh
authors.initials: WS
authors.lastname: Lakra
mesh_terms: D000818:Animals; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D007194:India; D007527:Isoenzymes / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D007527:Isoenzymes