Analysis of mRNA decay in Bacillus subtilis.
Studies of mRNA turnover in B. subtilis are less well known than in E. coli. Here we provide researchers who have an interest in gram-positive RNA processing with several protocols for RNA isolation, for 5'- and 3'-mapping of mRNAs and mRNA decay fragments, and we also include a comprehensive listing of B. subtilis mutants that are deficient in ribonucleases thought to be involved in mRNA decay.
Bechhofer
David H
DH
Mount Sinai School of Medicine of New York University, Department of Pharmacology and Systems Therapeutics, New York, USA.
Oussenko
Irina A
IA
Deikus
Gintaras
G
Yao
Shiyi
S
Mathy
Nathalie
N
Condon
Ciarán
C
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D004591:Electrophoresis, Polyacrylamide Gel; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger