Cation exchange separation of 61Cu2+ from natCo targets and preparation of 61Cu-DOTA-HSA as a blood pool agent.
An improved method for isolation of (61)Cu(2+) from a (nat)Co target using cation exchange was developed. (61)Cu(2+) was eluted from a cation exchange resin column by 0.2 M HCl with 90% acetone, while Co(2+) remained on the column. The whole separation process was completed within 50 min at more than 72% yield. The Co(2+) impurity level in (61)Cu(2+) solution was reduced to less than 0.1 ppm. Highly pure (61)Cu(2+) solution was then applied to prepare (61)Cu-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-human serum albumin (HSA) which showed good blood pool imaging properties.
Hao
Guiyang
G
Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
Fukumura
Toshimitsu
T
Nakao
Ryuji
R
Suzuki
Hisashi
H
Szelecsényi
Ferenc
F
Kovács
Zoltan
Z
Suzuki
Kazutoshi
K
D001345:Autoradiography; D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid; D003035:Cobalt / Q000737:chemistry*; D003301:Copper Radioisotopes; D015637:Gated Blood-Pool Imaging; D009942:Organometallic Compounds / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D014018:Tissue Distribution
C539909:61Cu-DOTA-HSA; D002411:Cation Exchange Resins; D003301:Copper Radioisotopes; D009942:Organometallic Compounds; D012709:Serum Albumin; D003035:Cobalt