title: Epileptogenesis alters gene expression pattern in rats subjected to amygdala-dependent emotional learning.
abstract: Here we tested a hypothesis that epileptogenesis influences expression pattern of genes in the basolateral amygdala that are critical for fear conditioning. Whole genome molecular profiling of basolateral rat amygdala was performed to compare the transcriptome changes underlying fear learning in epileptogenic and control animals. Our analysis revealed that after fear conditioning procedure 26 genes were regulated differently in the basolateral amygdala of both groups. Thus, our study provides the first evidence that not only the damage to the neuronal pathways but also altered composition or activity level of molecular machinery responsible for formation of emotional memories within surviving pathways can contribute to impairment in emotional learning in epileptogenic animals. Understanding the function of those genes in emotional learning provides an attractive avenue for identification of novel drug targets for treatment of emotional disorders after epileptogenesis-inducing insult.
authors.affiliation: Epilepsy Research Laboratory, Department of Neurobiology, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, PO Box 1627, FIN-70211, Kuopio, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Majak
authors.forename: M
authors.initials: M
authors.lastname: Dabrowski
authors.forename: A
authors.initials: A
authors.lastname: Pitkänen
mesh_terms: D000679:Amygdala / Q000503:physiopathology*; D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology; D003213:Conditioning, Psychological / Q000502:physiology*; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000009:adverse effects; D004827:Epilepsy / Q000209:etiology / Q000473:pathology*; D005239:Fear; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D042282:Microdissection / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley