[Studies on ethyl acetate soluble constituents of Huanglian Jiedutang].
OBJECTIVE
To study the ethyl acetate soluble constituents from the water extractive of Huanglian Jiedutang decoction, which are composed of Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri and Fructus Gardeniae, and provide substances foundation for its pharmacokinetic and pharmacodynamic investigation.


METHODS
The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS techniques.


RESULTS
Thirty-five compounds were isolated, among which twenty compounds have been identified as beta-sitosterol (1), oroxylin A (2), wogonin (3), ursolic acid (4), skullcapflavone I (5), tenaxin I (6), skullcapflavone II (7), limonin (8), 5, 2'-dihydroxy-6, 7, 8, 3'-tetramethoxyflavone (9), chrysin (12), baicalein (17), tenaxin II (19), 5, 7, 2'-trihydroxy-6, 8-dimethoxyflavone (21), shihulimonin A (22), 6, 2'-dihydroxy-5, 7, 8, 6'-tetramethoxyflavone (26), viscidulin II (28), 5, 7, 4'-trihydroxy-8-methoxyflavone (29), 5, 7, 2', 6'-tetrahydroxyflavone (30), wogonin-7-O-beta-D-glucuronide methyl ester (31) and daucosterol (34).


CONCLUSIONS
On the basis of reported results of the chemical constituents of Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri and Fructus Gardeniae, it was estimated that all flavonoid compounds rised from the Radix Scutellariae, and compounds 8 and 22 rised from Cortex Phellodendri. Compound 22 was identified in the Cortex Phellodendri for the first time.
Ma
Zhao-Tang
ZT
State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Yang
Xiu-Wei
XW
D000085:Acetates / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry; D047309:Flavones / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D036701:Limonins / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D012855:Sitosterols / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry; D000097245:Ursolic Acid
D000085:Acetates; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D036701:Limonins; D012855:Sitosterols; D014315:Triterpenes; D000097245:Ursolic Acid; C045607:huang-lien-chieh-tu-tang; C481180:shihulimonin A; C043561:chrysin; C006680:baicalein; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; C047405:skullcapflavone II; C007650:ethyl acetate; C506426:skullcapflavone I; C021255:stigmastanol; C001546:limonin; C085514:wogonin; C011015:lyoniside