Label-free electrochemical aptasensor for the detection of lysozyme.
This work reports the advantages of a label free electrochemical aptasensor for the detection of lysozyme. The biorecognition platform was obtained by the adsorption of the aptamer on the surface of a carbon paste electrode (CPE) previously blocked with mouse immunoglobulin under controlled-potential conditions. The recognition event was detected from the decrease in the guanine and adenine electro-oxidation signals produced as a consequence of the molecular interaction between the aptamer and lysozyme. The biosensing platform demonstrated to be highly selective even in the presence of large excess (9-fold) of bovine serum albumin, cytochrome C and myoglobin. The reproducibility for 10 repetitive determinations of 10.0 mg L(-1) lysozyme solution was 5.1% and 6.8% for guanine and adenine electro-oxidation signals, respectively. The detection limits of the aptasensor were 36.0 nmol L(-1) (if considering guanine signal) and 18.0 nmol L(-1) (if taking adenine oxidation current). This new sensing approach represents an interesting and promising alternative for the electrochemical quantification of lysozyme.
Rodríguez
Marcela C
MC
INFIQC, Departamento de Físico Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina. marcela.rodriguez@fcq.unc.edu.ar
Rivas
Gustavo A
GA
D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D009113:Muramidase / Q000032:analysis*; D015203:Reproducibility of Results
D052157:Aptamers, Nucleotide; D009113:Muramidase