title: Ran GTPase regulates hemocytic phagocytosis of shrimp by interaction with myosin.
abstract: Ran GTPases, one family of small G protein superfamily, have been widely demonstrated to be involved in the transport system between cytoplasm and the nucleus. However, the function of Ran GTPase in immunity remains unclear. In our study, it was found that the Ran GTPase (designated as PjRan) was up-regulated in virus-resistant shrimp, indicating that the PjRan might be implicated in the innate immune system against virus infection. On the basis of protein interactions, it was found that the PjRan interacted with myosin, a crucial protein in the process of phagocytosis to form a protein complex. The RNAi and mRNA assays showed that the PjRan could regulate shrimp hemocytic phagocytosis. Further data evidenced that the depletion of PjRan by RNAi caused a significant increase of virus copies, and the overexpression of PjRan resulted in a significant decrease of virus copies, suggesting that the PjRan participated in the antiviral immunity by regulating phagocytosis. Therefore, our study revealed a completely novel aspect of Ran GTPase in phagocytosis by the direct interaction with the cytoskeleton protein and presented a novel pathway concerning to antiviral immunity, which will help to better understand the molecular events in immune response against virus infection in invertebrates.
authors.affiliation: The Key Laboratory of Conservation Genetics and Reproductive Biology for Wild Animals of the Ministry of Education and College of Life Sciences, Zhejiang University, Hangzhou 310058, The People's Republic of China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006458:Hemolymph / Q000276:immunology / Q000821:virology; D007113:Immunity, Innate; D016253:Microscopy, Immunoelectron; D009218:Myosins / Q000378:metabolism*; D033561:Penaeidae / Q000276:immunology* / Q000648:ultrastructure / Q000821:virology; D010587:Phagocytosis; D014779:Virus Replication; D020931:ran GTP-Binding Protein / Q000502:physiology*
chemical_list: D009218:Myosins; D020931:ran GTP-Binding Protein