title: Effect of different vasodilators on NTPDase activity in healthy and hypertensive patients.
abstract: BACKGROUND
  The thrombogenic process that affects the hypertensive patient is associated with regulatory mechanisms present in the vascular endothelium. These mechanisms involve release of an endothelium-derived relaxing factor, ectonucleotidase activity and calcium ion concentration.
  
  
  METHODS
  Interference with ENTPDase activity in platelets of hypertensive patients and healthy donors was evaluated for arginine, sodium nitroprusside, and hydralazine. In addition, the kinetic behavior of NTPDase was determined in the presence of the vasodilator that showed the greatest inhibitory influence.
  
  
  RESULTS
  Vasodilators decreased NTPDase activity with ATP and ADP as substrates. In controls, hydrolysis was increased in the presence of arginine. Captopril did not affect enzyme activities. The dose response for increasing sodium nitroprusside was biphasic. Kinetic behavior studies were estimated in the presence of sodium nitroprusside, which caused a mixed inhibition. The K(m) values increased and V(max) decreased with increasing sodium nitroprusside concentrations. The IC(50) and K(i) values indicated that the vasodilator was a strong NTPDase inhibitor when tested for the control and hypertensive group, using ATP and ADP as substrate, respectively.
  
  
  CONCLUSIONS
  It is postulated that there was an interaction between vasodilators, NO donors and inhibition of NTPDase.
authors.affiliation: Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Daniéle Sausen
authors.initials: DS
authors.lastname: Lunkes
authors.forename: Gilberto Inácio
authors.initials: GI
authors.lastname: Lunkes
authors.forename: Mushtaq
authors.initials: M
authors.lastname: Ahmed
authors.forename: André L B
authors.initials: AL
authors.lastname: Morsch
authors.forename: Rafael Fernandes
authors.initials: RF
authors.lastname: Zanin
authors.forename: Paula Acosta
authors.initials: PA
authors.lastname: Maldonado
authors.forename: Maísa
authors.initials: M
authors.lastname: Corrêa
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
mesh_terms: D000251:Adenosine Triphosphatases / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D016896:Treatment Outcome; D014665:Vasodilator Agents / Q000008:administration & dosage*
chemical_list: D014665:Vasodilator Agents; D000251:Adenosine Triphosphatases