title: Antimicrobial activity of plant essential oils using food model media: efficacy, synergistic potential and interactions with food components.
abstract: The aim of this study was to optimise the antimicrobial efficacy of plant essential oils (EOs) for control of Listeria spp. and spoilage bacteria using food model media based on lettuce, meat and milk. The EOs evaluated were lemon balm, marjoram, oregano and thyme and their minimum inhibitory concentrations (MIC) were determined against Enterobacter spp., Listeria spp., Lactobacillus spp., and Pseudomonas spp. using the agar dilution method and/or the absorbance based microplate assay. MICs were significantly lower in lettuce and beef media than in TSB. Listeria strains were more sensitive than spoilage bacteria, and oregano and thyme were the most active EOs. EO combinations were investigated using the checkerboard method and Oregano combined with thyme had additive effects against spoilage organisms. Combining lemon balm with thyme yielded additive activity against Listeria strains. The effect of simple sugars and pH on antimicrobial efficacy of oregano and thyme was assessed in a beef extract and tomato serum model media. EOs retained greater efficacy at pH 5 and 2.32% sugar, but sugar concentrations above 5% did not negatively impact EO efficacy. In addition to proven antimicrobial efficacy, careful selection and investigation of EOs appropriate to the sensory profile of foods and composition of the food system is required. This work shows that EOs might be more effective against food-borne pathogens and spoilage bacteria when applied to foods containing a high protein level at acidic pH, as well as moderate levels of simple sugars.
authors.affiliation: School of Food Science and Environmental Health, Dublin Institute of Technology, Cathal Brugha Street, Dublin 1, Ireland.
authors.forename: J
authors.initials: J
authors.lastname: Gutierrez
authors.forename: C
authors.initials: C
authors.lastname: Barry-Ryan
authors.forename: P
authors.initials: P
authors.lastname: Bourke
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004754:Enterobacter / Q000187:drug effects / Q000254:growth & development; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D018545:Lactuca / Q000382:microbiology*; D008087:Listeria / Q000187:drug effects* / Q000254:growth & development; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D031341:Origanum / Q000737:chemistry; D010938:Plant Oils / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development; D013045:Species Specificity; D046930:Thymus Plant / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D010938:Plant Oils; C501365:lemon oil