[Rapid determination of contents of hirudin's hydrolysates in processed leech by dot blotting].
OBJECTIVE
To determine the contents of hirudin's hydrolysates in processed leeches, set up a new evaluating method of dot blotting to evaluate the qualities of those processed Chinese medicines as leeches.


METHODS
Contents of hirudin's hydrolysates in processed leeches were determined by dot blotting with rat antibody of anti-hirudin as the first antibody. Blotting signal was analyzed by software of Quantity One.


RESULTS
Contents of hirudin's hydrolysates in four batches of processed leeches were 296.51, 165.47, 95.58, and 298.05 microg g(-1), respectively.


CONCLUSIONS
Difference among four batches of processed leeches was significant in the content of hirudin's hydrolysates. Dot blotting, as a convenient and accurate method can be broadly used for evaluating processed products of Chinese crude drugs similar to leeches.
Wang
Hou-Wei
HW
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. houweiw@163.com
Tian
Jing-Zhen
JZ
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006629:Hirudins / Q000276:immunology / Q000378:metabolism*; D015151:Immunoblotting / Q000379:methods; D007865:Leeches / Q000737:chemistry*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D006629:Hirudins