title: Establishing and maintaining system linearity.
abstract: A flow cytometer system transforms a received optical signal into an electrical signal whose amplitude ideally is directly proportional to the optical signal but in practice is at least related in a linear fashion. Establishment and maintenance of system linearity are prerequisites to accurate quantitative measurements. The many potential sources of offsets and nonlinearity are found in all parts of the detection, amplification, and data acquisition systems of a flow instrument. This unit aims to help investigators evaluate performance of the hardware in flow cytometer detection systems.
authors.affiliation: Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA.
authors.forename: James C S
authors.initials: JC
authors.lastname: Wood
mesh_terms: D055615:Electrical Equipment and Supplies; D005434:Flow Cytometry / Q000295:instrumentation* / Q000592:standards*; D055095:Optics and Photonics / Q000295:instrumentation; D015203:Reproducibility of Results