title: MoisturePlus contact lens solution as a source of acanthamoeba keratitis.
abstract: Recently, it was suggested that the use of the Advanced Medical Optics Complete MoisturePlus multipurpose cleaning solution might be a risk factor for contracting Acanthamoeba keratitis, and the manufacturer voluntarily recalled it in May 2007. Polymerase chain reaction amplification and sequencing of a variable part of the 18S rRNA gene allowed for the first time tracking of Advanced Medical Optics Complete MoisturePlus-associated Acanthamoeba keratitis isolate from a patient back to the lens cleaning solution itself.
authors.affiliation: Department of Ophthalmology, Kantonsspital Lucerne, Lucerne, Switzerland. sih@gmx.net
authors.forename: Simon
authors.initials: S
authors.lastname: Hasler
authors.forename: Wasiliki
authors.initials: W
authors.lastname: Dedes
authors.forename: Alexander
authors.initials: A
authors.lastname: Mathis
authors.forename: Felix
authors.initials: F
authors.lastname: Grimm
authors.forename: Michael A
authors.initials: MA
authors.lastname: Thiel
mesh_terms: D000048:Acanthamoeba / Q000235:genetics; D015823:Acanthamoeba Keratitis / Q000209:etiology* / Q000473:pathology; D000368:Aged; D000818:Animals; D001483:Base Sequence; D018639:Contact Lens Solutions / Q000009:adverse effects*; D003315:Cornea / Q000382:microbiology; D016054:DNA, Protozoan / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S
chemical_list: D018639:Contact Lens Solutions; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S