Differential effects of triterpene glycosides, frondoside A and cucumarioside A2-2 isolated from sea cucumbers on caspase activation and apoptosis of human leukemia cells.
Frondoside A is a pentaoside having an acetyl moiety at the aglycon ring and xylose as a third monosaccharide residue. Cucumarioside A(2)-2 is a pentaoside having glucose as a third monosaccahride unit. We compared the effects of frondoside A and A(2)-2 for cell death-inducing capability with close attention paid to structure-activity relationships. Both frondoside A and A(2)-2 strongly induced apoptosis of leukemic cells. Frondoside A-induced apoptosis was more potent and rapid than A(2)-2-induced apoptosis. A(2)-2-induced but not frondoside A-induced apoptosis was caspase-dependent. This suggests that holothurians may induce apoptosis of leukemic cells caspase-dependently or -independently, depending on the holothurian structure.
Jin
Jun-O
JO
Department of Biochemistry, School of Medicine and Medical Research Center for Cancer Molecular Therapy, Dong-A University, #3-1 Dongdaeshin-dong, Seo-Ku, Busan 602-714, Republic of Korea.
Shastina
Valeria V
VV
Shin
Sung-Won
SW
Xu
Qi
Q
Park
Joo-In
JI
Rasskazov
Valery A
VA
Avilov
Sergey A
SA
Fedorov
Sergey N
SN
Stonik
Valentin A
VA
Kwak
Jong-Young
JY
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007928:Lethal Dose 50; D007938:Leukemia / Q000473:pathology*; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012616:Sea Cucumbers / Q000737:chemistry*; D013997:Time Factors; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
D017304:Annexin A5; D005456:Fluorescent Dyes; D006027:Glycosides; D012503:Saponins; D014315:Triterpenes; C539161:cucumarioside A2-2; C534797:frondoside A; C033504:cucumarioside; D020169:Caspases; D016650:Fluorescein-5-isothiocyanate