Lectin extracted from Canavalia grandiflora seeds presents potential anti-inflammatory and analgesic effects.
Neutrophil migration is responsible for tissue damage observed in inflammatory diseases and is also implicated in inflammatory nociception. The use of lectins has been demonstrated to be effective in different activities including anti-inflammatory, antimicrobial, and in cancer therapy. In this study, we addressed the potential use of a lectin from Canavalia grandiflora seeds (ConGF) to control neutrophil migration and inflammatory hypernociception. Pretreatment of the animals intravenously (15 min before) with ConGF inhibited neutrophil migration to the peritoneal cavity in a dose-dependent fashion confirmed by an inhibition of rolling and adhesion of leukocytes by intravital microscopy. Another set of experiments showed that pretreatment of the animals with ConGF inhibited the mechanical hypernociception in mice induced by the i.pl. injection of carrageenan or formalin. This anti-nociceptive effect correlated with an effective blockade of neutrophil influx, as assessed by the hind paw tissue myeloperoxidase levels. Furthermore, ConGF had important inhibitory effects on the mouse carrageenan-induced paw edema. In addition, animals treated with ConGF showed inhibition of cytokines release. In conclusion, we demonstrated that the lectin ConGF inhibits neutrophil migration and mechanical inflammatory hypernociception.
Nunes
Breno S
BS
Laboratory of Biopathology and Molecular Biology, University of Uberaba, Av. Nenê Sabino, 1801, Uberaba, Minas Gerais, 38055-500, Brazil.
Rensonnet
Nathalie S
NS
Dal-Secco
Daniela
D
Vieira
Silvio M
SM
Cavada
Benildo S
BS
Teixeira
Edson H
EH
Moura
Tales R
TR
Teixeira
Cícero S
CS
Clemente-Napimoga
Juliana T
JT
Cunha
Fernando Q
FQ
Napimoga
Marcelo H
MH
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D032449:Canavalia; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy / Q000473:pathology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds
D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D037121:Plant Lectins; C039268:lectin, Canavalia