Chemical initiation for butyl and lauryl acrylate monolithic columns for CEC.
Butyl acrylate (BA)- and lauryl acrylate (LA)-based monolithic stationary phases for CEC were synthesized, using a redox system as initiator of polymerization. BA monoliths were initiated with ammonium peroxodisulfate, whereas LA columns were obtained with lauroyl peroxide as initiator. In both cases, TEMED was used to activate the process. The influence of porogenic solvent composition on both morphological and electrochromatographic properties of the resulting monoliths was investigated. Excellent efficiencies (minimum plate heights of 4.2-6.3 microm for BA columns and 2.6-5.3 microm for LA stationary phases, for a PAHs mixture) were achieved. The capability of separation of both types of monolithic beds was compared by the analysis of complex mixtures of polycyclic aromatic hydrocarbons and anabolic steroids.
Cantó-Mirapeix
Amparo
A
Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Valencia, Spain.
Herrero-Martínez
José M
JM
Mongay-Fernández
Carlos
C
Simó-Alfonso
Ernesto F
EF
D000179:Acrylates / Q000737:chemistry*; D000645:Ammonium Sulfate / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000379:methods*; D005029:Ethylenediamines / Q000737:chemistry; D012739:Gonadal Steroid Hormones / Q000737:chemistry / Q000302:isolation & purification; D008054:Lipid Peroxides / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results
D000179:Acrylates; D005029:Ethylenediamines; D012739:Gonadal Steroid Hormones; D008054:Lipid Peroxides; D011084:Polycyclic Aromatic Hydrocarbons; C005798:N,N,N',N'-tetramethylethylenediamine; C031276:ammonium peroxydisulfate; C032490:n-butyl acrylate; C035089:lauroyl peroxide; D000645:Ammonium Sulfate