title: Detection of carbonylated proteins in two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis separations.
abstract: Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins.
authors.affiliation: Department of Chemistry, Center of Membrane Sciences, University of Kentucky, Lexington, KY, USA.
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Sultana
authors.forename: Shelley F
authors.initials: SF
authors.lastname: Newman
authors.forename: Quanzhen
authors.initials: Q
authors.lastname: Huang
authors.forename: D Allan
authors.initials: DA
authors.lastname: Butterfield
mesh_terms: D000818:Animals; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D021621:Imaging, Three-Dimensional; D015151:Immunoblotting; D007525:Isoelectric Focusing; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D011506:Proteins