Rapid and simultaneous determination of lycopene and beta-carotene contents in tomato juice by infrared spectroscopy.
The rapid quantification of lycopene and beta-carotene in tomato juices by attenuated total reflectance (ATR) infrared spectroscopy combined with multivariate analysis was evaluated. Two sample preparation methods were compared: a direct measurement of the tomato paste and an extraction method using hexane to isolate carotenoids. HPLC was used as the reference method. Cross-validated (leave-one-out) partial least-squares regression (PLSR) was used to create calibration models to predict these phytonutrient concentrations in blind test samples. The infrared spectra showed unique marker bands at 957 and 968 cm(-1) for lycopene and beta-carotene, respectively. Multivariate analysis of the infrared spectral data gave correlation coefficients (r values) of >0.9 between the ATR-IR predicted and HPLC reference values, and standard errors of cross-validation (SECV) of 0.5 and 0.04 mg/100 g of juice for lycopene and beta-carotene, respectively. ATR-IR could provide the tomato industry with a simple, rapid, and high-throughput technique for the determination of tomato quality.
De Nardo
Thais
T
Department of Food Science and Technology, 110 Parker Food Science and Technology Building, The Ohio State University, 2015 Fyffe Road, Columbus, Ohio 43210, USA.
Shiroma-Kian
Cecilia
C
Halim
Yuwana
Y
Francis
David
D
Rodriguez-Saona
Luis E
LE
D001628:Beverages / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D016018:Least-Squares Analysis; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D015999:Multivariate Analysis; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared / Q000379:methods*; D019207:beta Carotene / Q000032:analysis*
D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene