Aptamer selection express: a novel method for rapid single-step selection and sensing of aptamers.
Here we describe a new DNA capture element (DCE) sensing system, based on the quenching and dequenching of a double-stranded aptamer. This system shows very good sensitivity and thermal stability. While quenching, dequenching, and separating the DCE systems made from different aptamers (all selected by SELEX), an alternative method to rapidly select aptamers was developed-the Aptamer Selection Express (ASExp). This process has been used to select aptamers against different types of targets (Bacillus anthracis spores, Bacillus thuringiensis spores, MS-2 bacteriophage, ovalbumin, and botulinum neurotoxin). The DCE systems made from botulinum neurotoxin aptamers selected by ASExp have been investigated. The results of this investigation indicate that ASExp can be used to rapidly select aptamers for the DCE sensing system.
Fan
Maomian
M
Air Force Research Laboratories, RHPC, Brooks City-Base, TX, USA. maomian.fan@brooks.af.mil
McBurnett
Shelly Roper
SR
Andrews
Carrie J
CJ
Allman
Amity M
AM
Bruno
John G
JG
Kiel
Johnathan L
JL
D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics* / Q000302:isolation & purification*; D001408:Bacillus anthracis / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D001905:Botulinum Toxins / Q000235:genetics; D017909:Levivirus / Q000235:genetics; D010047:Ovalbumin / Q000235:genetics; D016133:Polymerase Chain Reaction; D052156:SELEX Aptamer Technique / Q000379:methods*; D013171:Spores, Bacterial / Q000235:genetics
D052157:Aptamers, Nucleotide; D010047:Ovalbumin; D001905:Botulinum Toxins