[Morroniside inhibits H2O2-induced apoptosis in cultured nerve cells].
OBJECTIVE
To investigate the effects of morroniside on H2O2-induced apoptosis in nerve cells.


METHODS
Human neuroblastoma cell line SH-SY5Y cells were pre-incubaed with morroniside (1, 10, and 100 micromol x L(-1)) for 24 h prior to exposure to H2O2 (500 micromol x L(-1)) for 18 h. The activity of reactive SOD was measured by a biochemical assay. The expression of caspase-3, caspase-9, Bcl-2 and Bax was determined by Wastern blotting method.


RESULTS
Pretreatment of the cells with morroniside (10 and 100 micromol x L(-1)) increasd SOD activity by 14% (P<0.01) and 11% (P<0.05) in comparison with cells exposed only to H2O2. Morroniside (1, 10, 100 micromol x L(-1)) lowered caspase-3 level by 31% (P<0.01), 103% (P<0.001) and 95% (P<0.001), decreased caspase-9 content by 71% (P<0.001), 132% (P<0.001) and 37% (P<0.05), and increasd Bcl-1 level by 88% (P<0.01), 121% (P<0.001) and 60% (P<0.01) respectively but no significant change occurred in Bax level in comparison with cells exposed only to H2O2.


CONCLUSIONS
Morroniside has neuroprotection effect against H2O2-induced oxidation injury in nerve cell.
Ai
Hou-Xi
HX
Department of Pharmacology, Xuan-wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.
Wang
Wen
W
Sun
Fang-Lin
FL
Huang
Wen-Ting
WT
An
Yi
Y
Li
Lin
L
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism
D006027:Glycosides; D016877:Oxidants; D019253:Proto-Oncogene Proteins c-bcl-2; C488401:morroniside; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D053148:Caspase 3; D053453:Caspase 9