Phage-mediated Shiga toxin 2 gene transfer in food and water.
Shiga toxin (stx) transduction in various food matrices has been evaluated with lysogens of Stx phages. stx transduction events were observed for many phages under appropriate conditions. Transduction did not occur at low pH and low temperatures. A total of 10(3) to 10(4) CFU ml(-1) was the minimal amount of donor and recipient strains necessary to generate transductants.
Imamovic
Lejla
L
Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
Jofre
Juan
J
Schmidt
Herbert
H
Serra-Moreno
Ruth
R
Muniesa
Maite
M
D001435:Bacteriophages / Q000235:genetics*; D004926:Escherichia coli / Q000821:virology; D005502:Food / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D022641:Shiga Toxin 2 / Q000235:genetics*; D012760:Shigella / Q000821:virology; D013696:Temperature; D014161:Transduction, Genetic; D037521:Virulence Factors / Q000235:genetics*; D014871:Water Microbiology
D022641:Shiga Toxin 2; D037521:Virulence Factors