title: Endodontic microorganism susceptibility by direct contact test.
abstract: The aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the Direct Contact Test. The sealers tested were: Endomethasone - Septodont, Endomethasone C-Septodont, Endion-Voco, Diaket-ESPE, Pulp Canal Sealer-SybronEndo, and AH26-Dentsply DeTrey. The endodontopathic microorganisms (MO) confronted were: Staphylococcus aureus (Sa), Candida albicans (Ca), Enterococcus faecalis (Ef), Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn). Test specimens of each sealer were prepared and placed on the surface of agar plates that had been inoculated with each MO, and after predetermined periods, transfers were made from the contact area between the test specimen and the cultured agar and from the area that had not been in contact with the test specimens (control). The results were read as presence/absence of microbial growth and analyzed statistically using the Kruskal-Wallis test. It was concluded that the structural features and virulence of endodontopathic microorganisms determine their response to the sealers, independently of the time during which sealers act and the mechanism by which the antiseptic reaches the microorganism, which in this case was by direct contact.
authors.affiliation: Department of Microbiology and Parasitology, School of Dentistry, University of Buenos Aires.
authors.forename: Sandra B
authors.initials: SB
authors.lastname: Pérez
authors.forename: Denise P
authors.initials: DP
authors.lastname: Tejerina
authors.forename: Romina I
authors.initials: RI
authors.lastname: Pérez Tito
authors.forename: Florencia L
authors.initials: FL
authors.lastname: Bozza
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Kaplan
authors.forename: Susana L
authors.initials: SL
authors.lastname: Molgatini
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001729:Bismuth / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D003407:Creosote / Q000494:pharmacology; D003788:Dental Pulp Diseases / Q000382:microbiology*; D003907:Dexamethasone / Q000494:pharmacology; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D004853:Epoxy Resins / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005566:Formocresols / Q000494:pharmacology; D016967:Fusobacterium nucleatum / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000494:pharmacology; D006801:Humans; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008828:Microbiological Techniques; D011145:Polyvinyls / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012834:Silver / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013943:Thymol / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology; D014774:Virulence; D015034:Zinc Oxide / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D004338:Drug Combinations; C098488:Endion; D004853:Epoxy Resins; D005566:Formocresols; D005899:Glass Ionomer Cements; D006847:Hydrocarbons, Iodinated; D011145:Polyvinyls; D012387:Root Canal Filling Materials; C011244:corticosteroid methanetriol mixture; D005557:Formaldehyde; D013943:Thymol; D012834:Silver; C028044:epoxy resin AH-26; C014457:bismuth phosphate, copolymers of vinyl acetate, dichlorophen, propionyl-acetophenone, triethanolamine, vinyl chloride, vinylisobutyl ether, zinc oxide drug combination; D003907:Dexamethasone; D003407:Creosote; C034472:Septodont; D014025:Titanium; D015034:Zinc Oxide; D001729:Bismuth; D006854:Hydrocortisone