Identification of streptothricin class antibiotics in the early-stage of antibiotics screening by electrospray ionization mass spectrometry.
Several streptothricin antibiotics have been studied by tandem mass spectrometry. The dominant product ions were derived from the C(7)-N bond cleavage which lead to lose streptolidine from the [M+H](+). The fragmentation pathways of key ions were described and certained by parent scan. According to the generalized principles, streptothricin isomers could be distinguished easily by the difference of CID spectra. A facile method based on ion-pair RP-HPLC coupled with electrospray ionization tandem mass spectrometry has been established for the analysis of streptothricins in the fermentation broth of Streptomyces qinlingensis. A total of 19 streptothricin-like compounds were identified or tentatively characterized based on their mass spectral data, and in which 11 were the first reported compounds. This could be used as an important de-replication method in the programs of screening novel antibiotics.
Ji
Zhiqin
Z
Institute of Pesticide Science, Northwest Agricultural & Forestry University, Shaanxi, China. wenjun_wu@263.net
Wei
Shaopeng
S
Zhang
Jiwen
J
Wu
Wenjun
W
Wang
Mingan
M
D000900:Anti-Bacterial Agents / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism*; D013309:Streptothricins / Q000032:analysis*
D000900:Anti-Bacterial Agents; D013309:Streptothricins