Care regimen and lens material influence on silicone hydrogel contact lens deposition.
OBJECTIVE
To quantitatively detect proteins and cholesterol extracted from worn silicone hydrogel contact lenses and determine the effect of various lens care solutions on deposit accumulation.


METHODS
Contact lenses, made from different polymers and worn on a daily wear schedule with different lens care solutions, were collected. Lipid and protein deposits were extracted by methanol:chloroform (1:1, v/v) and protein extraction solution (containing urea and surfactant), respectively. Lipid extracts were separated and cholesterol quantified using thin layer chromatography. Protein extracts were quantified using standard techniques.


RESULTS
Among all lenses tested, Balafilcon A lenses exhibited greatest extracted cholesterol (4.1 to 8.2 microg/lens) and total protein (5.4 to 23.2 microg/lens). AQuify was the most effective solution in reducing extracted deposits, especially extracted protein, from Balafilcon A lenses. AQuify and Opti-Free RepleniSH solutions were most effective in reducing extracted cholesterol from Senofilcon A and Galyfilcon A lenses, respectively. Use of Opti-Free Express solution resulted in more extracted protein from Lotrafilcon B lenses than use of other solutions. Generally, Lotrafilcon B, Senofilcon A, and Galyfilcon A lenses accumulated relatively low amount of proteins. Lotrafilcon B lenses accumulated the least amount of cholesterol deposit among all lenses tested regardless of solution used.


CONCLUSIONS
Lens polymer (possibly associated with surface characteristics) is a prominent factor affecting lipid and protein accumulation. Within a lens polymer type, lens care solutions exhibit varying effectiveness in reducing protein and lipid accumulation.
Zhao
Zhenjun
Z
Institute for Eye Research, Sydney, Australia. z.zhao@ier.org.au
Carnt
Nicole A
NA
Aliwarga
Yulina
Y
Wei
Xiaojia
X
Naduvilath
Thomas
T
Garrett
Qian
Q
Korth
John
J
Willcox
Mark D P
MD
D001672:Biocompatible Materials / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis*; D018639:Contact Lens Solutions / Q000494:pharmacology* / Q000592:standards; D003263:Contact Lenses, Hydrophilic; D005136:Eye Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels / Q000737:chemistry; D012828:Silicones / Q000737:chemistry
D001672:Biocompatible Materials; D018639:Contact Lens Solutions; D005136:Eye Proteins; D020100:Hydrogels; D012828:Silicones; C523195:balafilcon A; C524582:lotrafilcon B; C523196:senofilcon A; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002784:Cholesterol