title: Negative regulation of Caenorhabditis elegans epidermal damage responses by death-associated protein kinase.
abstract: Wounding of epidermal layers triggers multiple coordinated responses to damage. We show here that the Caenorhabditis elegans ortholog of the tumor suppressor death-associated protein kinase, dapk-1, acts as a previously undescribed negative regulator of barrier repair and innate immune responses to wounding. Loss of DAPK-1 function results in constitutive formation of scar-like structures in the cuticle, and up-regulation of innate immune responses to damage. Overexpression of DAPK-1 represses innate immune responses to needle wounding. Up-regulation of innate immune responses in dapk-1 requires the TIR-1/p38 signal transduction pathway; loss of function in this pathway synergizes with dapk-1 to drastically reduce adult lifespan. Our results reveal a previously undescribed function for the DAPK tumor suppressor family in regulation of epithelial damage responses.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Tong
authors.forename: Grace
authors.initials: G
authors.lastname: Lynn
authors.forename: Vy
authors.initials: V
authors.lastname: Ngo
authors.forename: Daniel
authors.initials: D
authors.lastname: Wong
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Moseley
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Ewbank
authors.forename: Alexandr
authors.initials: A
authors.lastname: Goncharov
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Wu
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pujol
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Chisholm
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000276:immunology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D064550:Death-Associated Protein Kinases; D007113:Immunity, Innate; D008854:Microscopy, Electron; D009154:Mutation; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases