title: Beneficial effect of the nonpeptidyl low molecular weight radical scavenger IAC on cultured human islet function.
abstract: We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan donors by collagenase digestion were purified by density gradient centrifugation. After the isolation, islets were either exposed or not exposed for 7 days to 10 micromol/L IAC. We found that IAC markedly reduced oxidative stress and ameliorated islets function. These results suggest that the use of IAC could be an interesting pharmacological approach for the treatment of the islets before transplantation.
authors.affiliation: Department of Endocrinology and Metabolism, Metabolic Unit, University of Pisa, Pisa, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Mancarella
authors.forename: Silvia
authors.initials: S
authors.lastname: Del Guerra
authors.forename: Matilde
authors.initials: M
authors.lastname: Masini
authors.forename: Marco
authors.initials: M
authors.lastname: Bugliani
authors.forename: Luca
authors.initials: L
authors.lastname: Valgimigli
authors.forename: Gian Franco
authors.initials: GF
authors.lastname: Pedulli
authors.forename: Moreno
authors.initials: M
authors.lastname: Paolini
authors.forename: Donatella
authors.initials: D
authors.lastname: Canistro
authors.forename: Andrea
authors.initials: A
authors.lastname: Armando
authors.forename: Antonio
authors.initials: A
authors.lastname: Soleti
authors.forename: Franco
authors.initials: F
authors.lastname: Filipponi
authors.forename: Franco
authors.initials: F
authors.lastname: Mosca
authors.forename: Ugo
authors.initials: U
authors.lastname: Boggi
authors.forename: Stefano
authors.initials: S
authors.lastname: Del Prato
authors.forename: Piero
authors.initials: P
authors.lastname: Marchetti
authors.forename: Roberto
authors.initials: R
authors.lastname: Lupi
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004952:Esters / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D018384:Oxidative Stress / Q000187:drug effects; D010880:Piperidines / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004952:Esters; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010880:Piperidines; C526136:bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate; C002744:3-nitrotyrosine; D014443:Tyrosine; D002374:Catalase; D013482:Superoxide Dismutase