title: Crystallization and preliminary X-ray diffraction analysis of GCIP/HHM transcriptional regulator.
abstract: GCIP/HHM is a human nuclear protein that is implicated in regulation of cell proliferation. Its primary structure contains helix-loop-helix and leucine-zipper motifs but lacks a DNA-binding basic region. Native and selenomethionine-derivatized (SeMet) crystals of full-length GCIP/HHM were obtained using the hanging-drop vapour-diffusion method. The crystals were greatly improved by adding tris(2-carboxyethyl)phosphine as a reducing reagent and diffracted to 3.5 A resolution. Preliminary phase calculations using the data set obtained from the SeMet crystal suggested that the crystal belonged to space group P3(2)21 and contained one molecule per asymmetric unit. Structure determination by the multiple-wavelength anomalous dispersion method using the SeMet crystals is in progress.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama-shi, Kanagawa, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Seto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ikushima
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Shuya
authors.initials: S
authors.lastname: Fukai
authors.forename: Keiko
authors.initials: K
authors.lastname: Yuki
authors.forename: Keiji
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Kohei
authors.initials: K
authors.lastname: Miyazono
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Ishitani
authors.forename: Osamu
authors.initials: O
authors.lastname: Nureki
mesh_terms: D049109:Cell Proliferation; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004247:DNA / Q000737:chemistry; D006801:Humans; D011485:Protein Binding; D012645:Selenomethionine / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: C485815:CCNDBP1 protein, human; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D004247:DNA; D012645:Selenomethionine