title: Comparative gene expression profile in two Atlantic salmon cell lines TO and SHK-1.
abstract: TO and SHK-1 are two Atlantic salmon (Salmo salar) cell lines isolated from head kidney cells and used widely in viral diagnostic and fundamental research. Analysis of their resting-state expression levels for a number of immune relevant genes revealed significant differences in expression between the two cell types in the absence of stimulant. TO cells expressed Arginase 1 beta and a CD4-related gene at a significantly higher level than SHK-1. In contrast, SHK-1 cells expressed type I interferon and one of its induced genes (Mx), gammaInterferon-induced Protein (gammaIP) and InterLeukin (IL)-10 at a significantly higher level than TO cells. These results suggest that these two cell lines, although originally isolated from the same organ have potentially very different functional properties. The SHK-1 cell line, or a subpopulation of SHK-1 cells, shares characteristics with dendritic cells whilst the TO cell line shows characteristics more traditionally associated with macrophages.
authors.affiliation: Fisheries Research Services, FRS Marine Laboratory, 375 Victoria Road, PO Box 101, Aberdeen AB119DB, Scotland, UK. colletb@marlab.ac.uk
authors.forename: Bertrand
authors.initials: B
authors.lastname: Collet
authors.forename: Catherine
authors.initials: C
authors.lastname: Collins
mesh_terms: D000818:Animals; D001119:Arginase / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D054357:Chemokine CXCL10 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D020869:Gene Expression Profiling / Q000662:veterinary; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007668:Kidney / Q000166:cytology / Q000502:physiology; D008264:Macrophages / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020317:Salmo salar / Q000235:genetics* / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; D054357:Chemokine CXCL10; D016753:Interleukin-10; D007371:Interferon-gamma; D001119:Arginase