[Studies on the effect of Ginkgo biloba extracts on NF-kappaB pathway].
OBJECTIVE
To investigate NF-kappaB and IkappaBalpha activities in HL-60 induced by TNF-alpha in order to understand the molecular mechanism of GbE in asthma treatment.


METHODS
The amount of IkappaBalpha in HL-60 cells stimulated by TNF-alpha and GbE was measured by western blotting. Plasmid pNF-kappaB-LuC was transfected and NF-kappaB activity was analyzed by measuring the expression level of luciferase.


RESULTS
It showed in the luciferase assay that the activity of NF-kappaB could significantly be suppressed in HL-60 cells after the pretreatment with CGbE. However, the phosphorylation and subsequent degradation of IKBalpha induced by TNF-alpha can not be inhibited in HL-60 cells even we prolonged the treatment time or increased the concentration of GhE.


CONCLUSIONS
GhE can suppress the NF-kappaB gene expression actively on independent of NIK/ IKK/ IkappaBalpha pathway in HL-60 cells.
Li
Guo-Hao
GH
The first Municipal People's Hospital of Guangzhou, Guangzhou 510180, China. ligh999@yahoo.com.cn
Lei
Xiu-Xia
XX
Yi
Yan-Mei
YM
Xu
Bang-Lao
BL
Wang
Hao-Ping
HP
Du
Jun
J
D001249:Asthma / Q000188:drug therapy; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D020441:Ginkgo biloba / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D016328:NF-kappa B / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010957:Plasmids; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D020672:I-kappa B Proteins; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha