[Genetic analysis and location of loci controlling leaf rust resistance of introgressive Triticum aestivum x Triticum timopheevii].
Introgressive lines resulting from crossing common wheat Triticum aestivum with the tetraploid T. timopheevii are characterized by effective resistance to leaf rust caused by Puccinia triticina Eriks. Molecular analysis using 350 specific short sequence repeat (SSR) markers was used to locate the T. timopheevii genome to chromosomes 1A, 2A, 2B, 5A, 5B, and 6B. A population of F2 offspring of crossing hybrid line 842-2 with common wheat cultivar Skala was obtained for mapping the loci controlling leaf rust resistance. Analysis of association of phenotypic and genotypic data by means of simple interval mapping (SIM) and composite interval mapping (CIM) has shown that the resistance of adult plants is determined by two loci in chromosomes 5B and 2A. The major locus QLr.icg-5B transferred from T. timopheevii chromosome 5G mapped to the interval of microsatellite loci Xgwm408-Xgwm1257 controls 72% of the phenotypic diversity of the trait. The other, minor locus QLr.icg-2A located to chromosome 2A at a distance of 10 cM from Xgwm312 accounts for 7% of the trait expression. Microsatellite markers located near these loci may be used for controlling the transfer of commercially valuable loci when new lines and cultivars are created.
Leonova
I N
IN
Roder
M S
MS
Kalinina
N P
NP
Budashkina
E B
EB
D002678:Chimera / Q000235:genetics* / Q000382:microbiology; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D011123:Polyploidy; D040641:Quantitative Trait Loci / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000382:microbiology