Effects of cartilage polysaccharide on apoptosis of human hepatoma BEL-7402 cells and murine H22 hepatocarcinoma.
OBJECTIVE
To detect the mechanism of apoptosis on hepatoma BEL-7402 cells induced by cartilage polysaccharide in vitro and the effect on H22 hepatocarcinoma mice in vivo.


METHODS
TUNEL assay, flow cytometry, immunofluorescent staining and animal experiments were used to examine the effects of cartilage polysaccharide on human hepatoma BEL-7402 cells in vitro or H22 hepatocarcinoma mice in vivo.


RESULTS
We found that cartilage polysaccharide had a dramatic effect on inducing apoptosis in BEL-7402 cells in a dose-dependent and time-dependent manner. The molecular mechanism of the process of apoptosis was investigated, which was correlated with FAS-caspase and regulated the expression level of Vimentin and p21 protein. The survival rate and lifespan of the experimental group were better than for the model group.


CONCLUSIONS
Our data demonstrate that cartilage polysaccharide may serve as a novel source of anti-hepatocarcinoma agent that may play an important role in future clinical trials.
Liu
Anjun
A
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Han
Xue
X
Zhang
Guorong
G
Zhao
Xihua
X
Zheng
Guoqiang
G
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002356:Cartilage / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D055808:Drug Discovery; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009363:Neoplasm Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D016019:Survival Analysis; D034421:Sus scrofa
D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D011134:Polysaccharides