Fast biosynthesis of GFP molecules: a single-molecule fluorescence study.
It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).
Katranidis
Alexandros
A
Forschungszentrum Jülich, ISB-2: Molecular Biophysics, 52425 Jülich, Germany.
Atta
Diaa
D
Schlesinger
Ramona
R
Nierhaus
Knud H
KH
Choli-Papadopoulou
Theodora
T
Gregor
Ingo
I
Gerrits
Michael
M
Büldt
Georg
G
Fitter
Jörg
J
D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D017510:Protein Folding; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins