title: Fast biosynthesis of GFP molecules: a single-molecule fluorescence study.
abstract: It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).
authors.affiliation: Forschungszentrum Jülich, ISB-2: Molecular Biophysics, 52425 Jülich, Germany.
authors.forename: Alexandros
authors.initials: A
authors.lastname: Katranidis
authors.forename: Diaa
authors.initials: D
authors.lastname: Atta
authors.forename: Ramona
authors.initials: R
authors.lastname: Schlesinger
authors.forename: Knud H
authors.initials: KH
authors.lastname: Nierhaus
authors.forename: Theodora
authors.initials: T
authors.lastname: Choli-Papadopoulou
authors.forename: Ingo
authors.initials: I
authors.lastname: Gregor
authors.forename: Michael
authors.initials: M
authors.lastname: Gerrits
authors.forename: Georg
authors.initials: G
authors.lastname: Büldt
authors.forename: Jörg
authors.initials: J
authors.lastname: Fitter
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D017510:Protein Folding; D012270:Ribosomes / Q000378:metabolism; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins