Beneficial effect of the nonpeptidyl low molecular weight radical scavenger IAC on cultured human islet function.
We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan donors by collagenase digestion were purified by density gradient centrifugation. After the isolation, islets were either exposed or not exposed for 7 days to 10 micromol/L IAC. We found that IAC markedly reduced oxidative stress and ameliorated islets function. These results suggest that the use of IAC could be an interesting pharmacological approach for the treatment of the islets before transplantation.
Mancarella
Rita
R
Department of Endocrinology and Metabolism, Metabolic Unit, University of Pisa, Pisa, Italy.
Del Guerra
Silvia
S
Masini
Matilde
M
Bugliani
Marco
M
Valgimigli
Luca
L
Pedulli
Gian Franco
GF
Paolini
Moreno
M
Canistro
Donatella
D
Armando
Andrea
A
Soleti
Antonio
A
Filipponi
Franco
F
Mosca
Franco
F
Boggi
Ugo
U
Del Prato
Stefano
S
Marchetti
Piero
P
Lupi
Roberto
R
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004952:Esters / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D018384:Oxidative Stress / Q000187:drug effects; D010880:Piperidines / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
D000975:Antioxidants; D004952:Esters; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010880:Piperidines; C526136:bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate; C002744:3-nitrotyrosine; D014443:Tyrosine; D002374:Catalase; D013482:Superoxide Dismutase