Disulfide-linked, gold nanoparticle based reagent for detecting small molecular weight thiols.
Disulfide-linked gold nanoparticles (AuNP) were synthesized by reacting dithiobis[succinimidylpropionate] (DSP) coated nanoparticles with glutathione disulfide. AuNP-cross-linking was monitored by the red shift and broadening of the AuNP's localized surface plasmon absorption resonance (LSPR) spectrum. The exposure of the disulfide-linked AuNPs to a variety of free thiols with systematically varying molecular weight revealed a AuNP-disulfide stability to reduction by thiols up to a critical molecular weight, M(c), of >300 Da thus making the disulfide-linked AuNP the first reagent that can discriminate thiols based on their size.
Durocher
Suzanne
S
Department of Chemistry & Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada.
Rezaee
Asad
A
Hamm
Caroline
C
Rangan
Chitra
C
Mittler
Silvia
S
Mutus
Bulent
B
D003124:Colorimetry / Q000379:methods; D004220:Disulfides / Q000138:chemical synthesis / Q000737:chemistry*; D019803:Glutathione Disulfide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D013388:Succinimides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry
D004220:Disulfides; D013388:Succinimides; D013438:Sulfhydryl Compounds; D006046:Gold; C011240:dithiobis(succinimidylpropionate); D019803:Glutathione Disulfide