Identification of potential recombination breakpoints in human parechoviruses.
Based on a comparison of the phylogeny of two distant regions, evidence has been found for recombination within parechoviruses. However, recombination breakpoints could not be detected in this way. We searched for potential recombination breakpoints in parechovirus by analysis of complete parechovirus sequences, including a newly isolated strain. Bootscan analysis demonstrated that parechoviruses are mosaic viruses build of regions related to corresponding genomic regions of other parechoviruses. With a genetic algorithm for recombination detection, sites for recombination were found. Analysis of partial sequences, as defined by recombination breakpoints, showed phylogenetic segregation between regions.
Zoll
Jan
J
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. j.zoll@ncmls.ru.nl
Galama
Jochem M D
JM
van Kuppeveld
Frank J M
FJ
D016000:Cluster Analysis; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000235:genetics*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D012367:RNA, Viral