Directed evolution of enzymes: Library screening strategies.
Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures.
Leemhuis
Hans
H
Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
Kelly
Ronan M
RM
Dijkhuizen
Lubbert
L