[Enhancement of norovirus detection rates in oysters and other food samples by using bacterial treatment].
Factors such as low recovery rate and food contaminants may be responsible for the difficulty of detecting Norovirus (NV) by PCR in foodborne outbreaks. To detect NV more efficiently, we introduced a bacterial treatment, in which concentrated samples were incubated overnight with Klebsiella oxytoca at 35 degrees C before RNA extraction using the standard protocol. Recovery rates of NVs (G I/8 or G II/13) added to food suspensions in the modified method were compared with those in the standard method by quantification of NV RNAs using real-time PCR. Recovery rates in the modified method were 8.6% for G I/8 and 11.6% for G II/13 in 18 oyster samples and 13.9% for G I/8 and 19.6% for G II/13 in 15 other food samples, while those in the standard method were 0.3% for G I/8 and 0.5% for G II/13 in the oyster samples and 1.9% for G I/8 and 7.9% for G II/13 in the other food samples. These results indicate that the bacterial treatment increase the recovery of NV from foods such as oysters, suggesting that the modified method will be useful for NV detection in food samples.
Akiba
Tetsuya
T
Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Tanaka
Tatsuya
T
Arai
Teruyoshi
T
Hayashi
Yukinao
Y
Mori
Kohji
K
Noguchi
Yayoi
Y
Nagano
Miyuki
M
Yoshida
Yasuko
Y
Yano
Kazuyoshi
K
D000818:Animals; D005516:Food Microbiology; D041121:Klebsiella oxytoca; D008828:Microbiological Techniques; D029322:Norovirus / Q000302:isolation & purification*; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral
D012367:RNA, Viral