N-glycosylation of plant recombinant pharmaceuticals.
N-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is N-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry, N-glycan profile analysis, and glycopeptide identification by LC-MS.
Bardor
Muriel
M
Faculté des Sciences, Université de Rouen, Mont Saint Aignan, France.
Cabrera
Gleysin
G
Stadlmann
Johannes
J
Lerouge
Patrice
P
Cremata
José A
JA
Gomord
Véronique
V
Fitchette
Anne-Catherine
AC
D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D006801:Humans; D004364:Pharmaceutical Preparations; D010944:Plants / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004364:Pharmaceutical Preparations; D011994:Recombinant Proteins