Use of multiple-reaction monitoring ratios for identifying incompletely resolved fentanyl homologs and analogs via ultra-high-pressure liquid chromatography-tandem mass spectrometry.
Fentanyl and 16 of its corresponding homologs and analogs were distinguished using ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A 1.7 microm Acquity BEH C18 column (150 mm x 2.1 mm) was used with a 1% formic acid (pH 2.2), methanol gradient. Multiple-reaction monitoring (MRM) was employed for MS/MS detection. All selected fentanyl-related compounds, including incompletely resolved compounds, were uniquely identified using retention times and dual MRMs.
Lurie
Ira S
IS
Special Testing and Research Laboratory, US Drug Enforcement Administration, Dulles, VA 20166, USA. islurie@comcast.net
Iio
Reiko
R
D002853:Chromatography, Liquid / Q000379:methods*; D005283:Fentanyl / Q000031:analogs & derivatives* / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005283:Fentanyl