title: Influence of germination with different selenium solutions on nutritional value and cytotoxicity of lupin seeds.
abstract: The effect of different selenium solutions during germination of lupin seeds (Lupinus angustifolius L. cv. Zapaton) on the content of total selenium, protein, amino acids, soluble carbohydrates, total antioxidant activity, and cytotoxicity on HL-60 human leukemic cell line has been studied. Seeds were germinated in the presence of selenite (Na2SeO3) or selenate (Na2SeO4) solutions at different concentrations (0, 2, 4, 6, and 8 mg/L) for 5 days at either 20 or 25 degrees C. The addition of inorganic Se forms significantly increased Se content in lupin sprouts in a dose-dependent manner. The highest Se content in lupin sprouts was observed when germination was carried out with selenate solutions at 20 degrees C (11 microg/g of dw) or 25 degrees C (14 microg/g of dw). The Se-enriched sprouts presented an improvement in antioxidant activity (up to 117.8 and 103.5 micromol of Trolox/g of dw) as well as in essential amino acid content, and no cytotoxicity was observed on HL-60 human leukemic cells. Lupin seeds germinated with 8 mg/L selenate solutions for 5 days at 20 degrees C exhibited a higher germination rate (>90%) and a higher concentration of some essential amino acids than those obtained in selenite solutions in the same germination conditions. Therefore, the employment of selenate solutions at a concentration of 8 mg/L and germination for 5 days at 20 degrees C may be suggested for the production of Se-enriched lupin sprouts.
authors.affiliation: Instituto de Fermentaciones Industriales and Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Juana
authors.initials: J
authors.lastname: Frias
authors.forename: Piotr
authors.initials: P
authors.lastname: Gulewicz
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Villaluenga
authors.forename: Radosław
authors.initials: R
authors.lastname: Pilarski
authors.forename: Enrique
authors.initials: E
authors.lastname: Blazquez
authors.forename: Begoña
authors.initials: B
authors.lastname: Jiménez
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gulewicz
authors.forename: Concepción
authors.initials: C
authors.lastname: Vidal-Valverde
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002241:Carbohydrates; D018525:Germination / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D031297:Lupinus; D009753:Nutritive Value; D012639:Seeds / Q000737:chemistry / Q000254:growth & development*; D064586:Selenic Acid; D012643:Selenium / Q000008:administration & dosage* / Q000032:analysis; D018036:Selenium Compounds / Q000008:administration & dosage; D012996:Solutions
chemical_list: D000596:Amino Acids; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D002241:Carbohydrates; D018036:Selenium Compounds; D012996:Solutions; D012643:Selenium; D064586:Selenic Acid