Effect of alpha-tocopherol on the synthesis of phosphatidylglycerol catalyzed by phospholipase D in an aqueous system.
Phosphatidylglycerol (PG) was synthesized from several phosphatidylcholines (PCs) via phospholipase D (PLD)-catalyzed transphosphatidylation in an aqueous system. The yield of PG were 71 and 68 mol% from soybean PC and egg yolk PC, respectively, under the optimum reaction conditions of 50 micromol PC, 10 mmol glycerol, 3 ml of acetate buffer, 1.6 U PLD, and 30 micromol CaCl(2) at 37 degrees C for 48 h. In case of salmon roe PC with 14.3% eicosapentaenoic acid and 26.8% docosahexaenoic acid, the PG yield increased to 94 mol% by addition of 46 mumol alpha-tocopherol, although the PG yield was only 10% in absence of alpha-tocopherol.
Suzuri
Kounosuke
K
Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato, Hakodate, Hokkaido, 041-8611, Japan.
Yamamoto
Yukihiro
Y
Hosokawa
Masashi
M
Miyashita
Kazuo
K
D000818:Animals; D004281:Docosahexaenoic Acids / Q000378:metabolism; D004530:Egg Yolk / Q000737:chemistry; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D010713:Phosphatidylcholines / Q000302:isolation & purification / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D012474:Salmon; D013025:Glycine max / Q000737:chemistry; D024502:alpha-Tocopherol / Q000378:metabolism*
D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D010739:Phospholipase D; D024502:alpha-Tocopherol