Quantitative analysis of short-chain acyl-coenzymeAs in plant tissues by LC-MS-MS electrospray ionization method.
Because acyl-CoAs play major roles in numerous anabolic and catabolic pathways, the quantitative determination of these metabolites in biological tissues is paramount to understanding the regulation of these metabolic processes. Here, we report a method for the analysis of a collection of short-chain acyl-CoAs (<6 carbon chain length) from plant extracts. Identification of each individual acyl-CoA was conducted by monitoring specific mass-fragmentation ions that are derived from common chemical moieties of all Coenzyme A (CoA) derivatives, namely the adenosine triphosphate nucleotide, pantothenate and acylated cysteamine. This method is robust and quick, enabling the quantitative analysis of up to 12 different acyl-CoAs in plant metabolite extracts with minimal post-extraction processing, using a 30min chromatographic run-time.
Perera
M Ann D N
MA
W. M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, IA 50011, United States.
Choi
Suh-Yeon
SY
Wurtele
Eve Syrkin
ES
Nikolau
Basil J
BJ
D000214:Acyl Coenzyme A / Q000032:analysis* / Q000737:chemistry; D017360:Arabidopsis / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D009928:Organ Specificity; D018515:Plant Leaves / Q000737:chemistry; D012015:Reference Standards; D036226:Seedlings / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000214:Acyl Coenzyme A