Prevalence of Actinobacillus actinomycetemcomitans in Chinese chronic periodontitis patients and periodontally healthy adults.
OBJECTIVE
Actinobacillusactinomycetemcomitans is a major periodontal pathogen. This research was aimed at investigating the prevalence of A actinomycetemcomitans in Chinese chronic periodontitis patients and periodontally healthy adults.


METHODS
A total of 116 chronic periodontitis patients and 111 periodontally healthy adults were included. In each periodontitispatient,subgingival plaque samples were collected from 2 molar sites with the deepest pockets and 1 periodontally healthy site. The samples of periodontally healthy adults were obtained from the mesiobuccal site of 1 maxillary first molar. A actinomycetemcomitans was detected by 16S rRNA polymerase chain action. Pocket depth, clinical attachment loss, and bleeding on probing of the sampled sites were recorded.


RESULTS
A actinomycetemcomitans was detected in only 1 site (0.90%) of periodontally healthy subjects. For chronic periodontitispatients, the prevalence of periodontitis sites (33.62%) was significantly higher than that of healthy sites (0.90%)(P<.05); the occurrence showed a decreasing trend as patient age increased: highest in the 20- to 35-year-old group (44.12%), followed by the 36- to 55-year-old group (36.36%) and 56- to 75-year-old group (22.73%)(P<.05). A actinomycetemcomitans was most frequently detected in sites with pocket depth 7 mm or more and clinical attachment loss 6 mm or more (P<.05) and more often detected in sites that exhibited bleeding on probing (37.07%) than those that did not (7.41%)(P<.05).


CONCLUSIONS
A actinmycetemcomitans was more frequently detected in periodontitis sites than in periodontally healthy sites. For chronic periodontitispatients, a higher prevalence was associated with the severe sites than moderate and mild sites. A actinomycetemcomitans is considered to be a major pathogen in the etiology of chronic periodontitis
Meng
Shu
S
Zhao
Lei
L
Yang
He
H
Wu
Yafei
Y
Ouyang
Yuling
Y
D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification / Q000472:pathogenicity*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D055113:Chronic Periodontitis / Q000453:epidemiology / Q000382:microbiology*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D055815:Young Adult
D004269:DNA, Bacterial