title: Comparative analysis of the sialic acid binding activity and the tropism for the respiratory epithelium of four different strains of avian infectious bronchitis virus.
abstract: Avian infectious bronchitis virus (IBV) is a major pathogen in commercial poultry flocks. We recently demonstrated that sialic acid serves as a receptor determinant for IBV on the tracheal epithelium. Here we compared the IBV strains Beaudette, 4/91, Italy02, and QX for their sialic acid-binding properties. We demonstrate that sialic acid binding is important for the infection of primary chicken kidney cells and the tracheal epithelium by all four strains. There were only slight differences between the four strains, indicating the universal usage of sialic acids as receptor determinants by IBV. In addition, we analysed the primary target cells in the respiratory epithelium of the four different strains and found that all of them infected ciliated cells and goblet cells.
authors.affiliation: Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Abd El Rahman
authors.forename: A A
authors.initials: AA
authors.lastname: El-Kenawy
authors.forename: U
authors.initials: U
authors.lastname: Neumann
authors.forename: G
authors.initials: G
authors.lastname: Herrler
authors.forename: C
authors.initials: C
authors.lastname: Winter
mesh_terms: D000818:Animals; D002642:Chick Embryo; D001351:Infectious bronchitis virus / Q000145:classification* / Q000502:physiology*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000166:cytology / Q000821:virology*; D014132:Trachea / Q000166:cytology; D018523:Tropism / Q000502:physiology*
chemical_list: D019158:N-Acetylneuraminic Acid