Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts.
The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing of organelle transcripts. We discovered that the DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo. Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins. In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2. We propose that different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing.
Okuda
Kenji
K
Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502 Japan.
Chateigner-Boutin
Anne-Laure
AL
Nakamura
Takahiro
T
Delannoy
Etienne
E
Sugita
Mamoru
M
Myouga
Fumiyoshi
F
Motohashi
Reiko
R
Shinozaki
Kazuo
K
Small
Ian
I
Shikanai
Toshiharu
T
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017393:RNA Editing; D015347:RNA Probes; D018749:RNA, Plant / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D015347:RNA Probes; D018749:RNA, Plant; D011994:Recombinant Proteins