title: Anastomotic healing in ileum and colon of alloxan-induced diabetic rats.
abstract: OBJECTIVE
  To investigate if diabetes mellitus may alter breaking strength (BS) and collagen content in ileum and colon anastomoses in rats.
  
  
  METHODS
  Three-hundred Wistar rats were randomly assigned to 5 experimental groups, 60 per group: normal controls surgically manipulated (G1); normal controls submitted to ileum (G2) and colon (G3) anastomotic construction; diabetic rats submitted to ileum (G4) and colon (G5) anastomotic construction. Each group was further divided into 6 subgroups with 10 rats each for sacrifice at 0, 4, 7, 14, 21, and 30 days after surgery. All surgical procedures were performed 3 months after alloxan diabetes induction. BS was measured in all intestinal anastomoses. Fragments of ileum and colon anastomoses were taken for hydroxyproline concentration (HP) and total tissue protein (TP) dosages.
  
  
  RESULTS
  Anastomotic BS was significantly decreased (P<0.05) in ileum and colon of G4 and G5 diabetic groups up to 7 and 14 days after surgery, respectively, compared with G2 and G3 normal control groups. Anastomotic HP and TP content did not significantly differ between diabetic and normal control operated groups in ileum or colon at all evaluation times.
  
  
  CONCLUSIONS
  Experimental diabetes leads to impaired intestinal anastomotic strength during early surgical wound repair, but does not appear to be implicated with collagen synthesis capacity.
authors.affiliation: Department of Surgery, Botucatu School of Medicine, Sao Paulo State University, Brazil.
authors.forename: José Lúcio Martins
authors.initials: JL
authors.lastname: Machado
authors.forename: Erika Veruska Paiva
authors.initials: EV
authors.lastname: Ortolan
authors.forename: César Tadeu
authors.initials: CT
authors.lastname: Spadella
mesh_terms: D000496:Alloxan; D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D003106:Colon / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004195:Disease Models, Animal; D007082:Ileum / Q000601:surgery*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000496:Alloxan; D003094:Collagen