title: Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons.
abstract: Spectral properties of a recently developed voltage-sensitive dye, di-4-ANEPPDHQ, were characterized as the dye was dissolved in the solvent dimethyl sulfoxide as the stock solution, in Hank's buffered salt solution as the staining solution, and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons (GT1-7) in vitro. Their dependence on the local chemical and electrical environment of dye molecules was determined. The excitation and emission peaks are 479 nm and 570 nm for the stained primary neurons, and 476 nm and 585 nm for the stained immortalized neurons. The excitation and emission bands of the stained GT1-7 neurons, defined as 50% peak intensity, are 429-516 nm and 544-648 nm, respectively.
authors.affiliation: Center for Optical Technologies, Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA 18015, USA. yuw207@lehigh.edu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Gaoshan
authors.initials: G
authors.lastname: Jing
authors.forename: Susan
authors.initials: S
authors.lastname: Perry
authors.forename: Filbert
authors.initials: F
authors.lastname: Bartoli
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tatic-Lucic
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry*; D004560:Electricity; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000166:cytology*; D011726:Pyridinium Compounds / Q000737:chemistry*; D051381:Rats; D012996:Solutions; D013057:Spectrum Analysis
chemical_list: D004396:Coloring Agents; D011726:Pyridinium Compounds; D012996:Solutions; C541550:di-4-ANEPPDHQ