[The hepatoprotective effect of aqueous extracts from Ficus hirta on N, N-dimethylformamide induced acute liver injury in mice].
OBJECTIVE
To investigate the effect of aqueous extract from Ficus hirta on N, N-Dimethylformamide (DMF) induced liver injury in mice.


METHODS
C57BL/6 mice and ICR mice were randomly divided into 5 groups: negative control group, positive control group and three treated groups respectively. Treated groups were administered orally with 100, 200, 300 g/kg Ficus hirta aqueous extract per day respectively for 5 days. On the 4th day, 2. 3 g/kg DMF was given by intraperitoneal injection to all C57BL/6 mice except negative control group, while for ICR, DMF was administration at a 2.75 g/kg dose. 48h after DMF injection, serum samples were collected to determine the activities of ALT, AST and LDH and the pathological changes of liver tissue were analyzed under microscope.


RESULTS
Compared with the positive control, the activities of ALT, AST and LDH were significantly reduced and the liver injury obviously attenuated in treated groups.


CONCLUSIONS
The aqueous extract of Ficus hirta has an obvious protective effect against DMF-induced acute liver injury in mice.
Lv
Ying-Jian
YJ
Peking University Health Science Center, Beijing 100083, China.
Jia
Feng-Lan
FL
Ruan
Ming
M
Zhang
Bao-Xu
BX
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000517:prevention & control*; D004126:Dimethylformamide / Q000506:poisoning; D004305:Dose-Response Relationship, Drug; D005260:Female; D030681:Ficus / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation
D010936:Plant Extracts; D020011:Protective Agents; D004126:Dimethylformamide; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase