title: The Rv1712 Locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP.
abstract: The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed.
authors.affiliation: Av. Ipiranga 6681, Tecnopuc-Prédio 92A, Porto Alegre, RS 90619-900, Brazil. luiz.basso@pucrs.br.
authors.forename: Caroline
authors.initials: C
authors.lastname: Thum
authors.forename: Cristopher Z
authors.initials: CZ
authors.lastname: Schneider
authors.forename: Mario S
authors.initials: MS
authors.lastname: Palma
authors.forename: Diógenes S
authors.initials: DS
authors.lastname: Santos
authors.forename: Luiz A
authors.initials: LA
authors.lastname: Basso
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003843:Deoxycytidine Monophosphate / Q000378:metabolism*; D015870:Gene Expression; D007700:Kinetics; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics*; D009703:Nucleoside-Phosphate Kinase / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011994:Recombinant Proteins; D003843:Deoxycytidine Monophosphate; C019979:cytidylate kinase; D009703:Nucleoside-Phosphate Kinase