title: A type-specific study of human papillomavirus prevalence in cervicovaginal samples in three different Spanish regions.
abstract: Human papillomavirus (HPV) is the most frequent sexually transmitted viral infection. It is necessary to know HPV genotype distribution to identify how many women will be protected by HPV vaccines. During a period of 18 months, we have analyzed 2362 HPV positive reporting data from a secondary demand screening program in three regions in Spain (Cantabria, Leon and Burgos). The study has been conducted using polymerase chain reaction and tube array hybridization covering the 35 HPV genotypes described as affecting anogenital mucosa. There were no significant differences between the three regions according to genotype distribution. The most frequent were HPV16 (19.18%), HPV53 (11.26%) and HPV58 (7.66%). HPV18 was the source of 4.02% of infections. High-risk HPVs were found in 1863/2362 cases. HPV16 was present in 24.3% of high-risk infections and HPV18 was found in 5.1%. Uncommon genotypes (<5% of the total prevalence each) were found in 17,9% of the total high-risk infections (334/1863). Multiple infections were diagnosed in 22% of the cases. The HPV genotype distribution is different from previously published data when multiple types are included in the screening. Both HPV16/18 account for 30% of high-risk infections in a clinical setting in Spain. The presence of multiple genotypes is very common among the population.
authors.affiliation: Departamento de Anatomia Patológica, Hospital Universitario Marqués de Valdecilla, Facultad de Medicina, Universidad de Cantabria, Santander, Spain. apagrj@humv.es
authors.forename: Jose Javier
authors.initials: JJ
authors.lastname: Gomez-Roman
authors.forename: Celina
authors.initials: C
authors.lastname: Echevarria
authors.forename: Santos
authors.initials: S
authors.lastname: Salas
authors.forename: María Asunción
authors.initials: MA
authors.lastname: González-Morán
authors.forename: Belen
authors.initials: B
authors.lastname: Perez-Mies
authors.forename: Isabel
authors.initials: I
authors.lastname: García-Higuera
authors.forename: Montserrat
authors.initials: M
authors.lastname: Nicolás Martínez
authors.forename: Jose Fernando
authors.initials: JF
authors.lastname: Val-Bernal
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D009092:Mucous Membrane / Q000821:virology; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D013030:Spain / Q000453:epidemiology; D013045:Species Specificity; D014621:Vagina / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D004279:DNA, Viral