title: The future of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discovery.
abstract: The future utility of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discover will be discussed, beginning with a brief description of the evolution of metabolomics and the utilization of the three most popular analytical platforms in such studies: NMR, GC-MS, and LC-MS. Emphasis is placed on recent developments in high-efficiency LC separations, sensitive electrospray ionization approaches, and the benefits to incorporating both in LC-MS-based approaches. The advantages and disadvantages of various quantitative approaches are reviewed, followed by the current LC-MS-based tools available for candidate biomarker characterization and identification. Finally, a brief prediction on the future path of LC-MS-based methods in metabolic profiling and metabolomic studies is given.
authors.affiliation: Biological Science Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
authors.forename: Qibin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jason S
authors.initials: JS
authors.lastname: Page
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Shen
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Callister
authors.forename: Jon M
authors.initials: JM
authors.lastname: Jacobs
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith