Improvement of cytocompatibility of electrospinning PLLA microfibers by blending PVP.
In this study, microfiber films were used as scaffolds for the purpose of vascular tissue engineering. The microfiber films were prepared by electrospinning of poly (l-lactide) (PLLA) and polyvinyl pyrrolidone (PVP). PLLA and PVP with different ratios were blended with dichloromethane as a spinning solvent at room temperature. The properties of the composite microfiber films were investigated by differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and contact angle measurement. The SEM images showed that the morphology of the microfiber films was mainly affected by the weight ratios of PLLA/PVP. The DSC results demonstrated that PLLA and PVP mixed uniformly. And the hydrophilicity of the films measured increased along with the decrease of the PLLA/PVP ratio. Vascular smooth muscle cells (VSMCs) were used to test the cytocompatibility. Cell morphology and cell proliferation were measured by SEM, laser scanning confocal microscopy (LSCM) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay after 2, 4, 6 days of culture. The results indicated that the cell morphology and proliferation on the composite films were better than that on the pure PLLA film. Furthermore, morphology and proliferation of VSMCs became better with decreasing of the weight ratio of PLLA/PVP. In addition, adhesion of platelet on the films was observed by SEM. The SEM images showed that the number of adhered platelets decreased with increment of PVP content in the films. The electrospinning microfiber composite films of PLLA and PVP would have potential use as the scaffolds for vascular tissue engineering.
Xu
Fei
F
Department of Materials Science and Engineering, Tsinghua University, Beijing, China.
Cui
Fu-Zhai
FZ
Jiao
Yan-Peng
YP
Meng
Qing-Yuan
QY
Wang
Xiao-Ping
XP
Cui
Xi-Yun
XY
D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004396:Coloring Agents / Q000378:metabolism; D006801:Humans; D008752:Methylene Chloride / Q000737:chemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D010973:Platelet Adhesiveness; D011091:Polyesters / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry*; D012997:Solvents; D013696:Temperature; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors
D001672:Biocompatible Materials; D004396:Coloring Agents; D011091:Polyesters; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; C033616:poly(lactide); D008752:Methylene Chloride; C022616:thiazolyl blue; D011205:Povidone