Contralateral leg as a control during skeletal muscle ischemia-reperfusion.
BACKGROUND
Recent data demonstrated that hind limb ischemia induces skeletal muscle mitochondrial dysfunctions. Improvement of such metabolic myopathy improves patient's symptomatology, supporting the development of experimental models focused on mitochondrial function analysis. However, although the nonischemic contralateral leg is often used as a control during unilateral leg ischemia, whether it might be useful when assessing ischemia-induced mitochondrial dysfunction remains to be investigated.


METHODS
Both ischemic (IR) and nonischemic contralateral legs (CTL) of rats (n=13) submitted to 5 h ischemia induced by a rubber band tourniquet applied on the root of the hind limb were studied and compared to that of sham-operated animals (SHAM, n=13). Maximal oxidative capacities (V(max)) and complexes I, II and IV activities of the gastrocnemius mitochondrial respiratory chain were determined, using glutamate-malate, succinate (Vs) and TMPD-ascorbate (V(TMPD)) substrates.


RESULTS
V(max) was decreased in IR (4.6+/-0.4 microM/min/g dry weight) compared to both SHAM and CTL muscles (8.5+/-0.5 and 7.1+/-0.4 microM/min/g dry weight, -46% and -36%, P<0.001, respectively). V(S) and V(TMPD) were reduced in IR muscle (-56% and -48% for V(S); and -25% and -24% for V(TMPD), P<0.001) as compared to SHAM and CTL). V(S) and V(TMPD) were similar in SHAM and CTL muscles.


CONCLUSIONS
Five hours ischemia-reperfusion significantly impaired complexes I, II and IV of the ischemic skeletal muscle mitochondrial respiratory chain. Interestingly, only V(max) was slightly altered in the contralateral leg, supporting that the nonischemic leg might be used as a control when assessing mitochondrial function in the experimental setting of unilateral hind limb ischemia.
Thaveau
Fabien
F
Department of Vascular Surgery, Hopital Civil, Strasbourg, France.
Zoll
Joffrey
J
Bouitbir
Jamal
J
Ribera
Florence
F
Di Marco
Paola
P
Chakfe
Nabil
N
Kretz
Jean Georges
JG
Piquard
Francois
F
Geny
Bernard
B
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D019069:Cell Respiration; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D007511:Ischemia / Q000150:complications* / Q000378:metabolism; D035002:Lower Extremity / Q000098:blood supply*; D008293:Malates / Q000378:metabolism; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000209:etiology* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism; D013772:Tetramethylphenylenediamine
D045222:Electron Transport Chain Complex Proteins; D008293:Malates; D018698:Glutamic Acid; C030298:malic acid; D019802:Succinic Acid; D013772:Tetramethylphenylenediamine; D001205:Ascorbic Acid