Effects of mitomycin C on infiltration of polymorphonuclear leukocytes after epithelial scrape injury in the mouse cornea.
OBJECTIVE
To determine whether mitomycin C (MMC) alters appearance and disappearance of polymorphonuclear leucocytes (PMN) in the cornea stroma, using an epithelial scrape injury in eye mouse model.


METHODS
Twenty-mice underwent mechanical epithelium debridement in the central cornea using 20% ethanol. After the scrape, the right eye received 0.02% MMC for one minute, while the left eye received physiological saline. The animals were sacrificed on days 1, 2, 5, and 14 after surgery, and corneal whole mounts were prepared for histology. PMN distribution was analyzed in digitized microscope images. Cell division in the cornea was determined by immunohistochemical detection of bromodeoxyuridine (BrdU), which was injected intraperitoneally before the mice were sacrificed.


RESULTS
Epithelial scrape injury triggered infiltration of PMNs into the corneal stroma. An analysis of PMN distribution revealed that there was no difference between eyes treated with and without MMC at all time points. BrdU labeling showed that 0.02% MMC for one minute blocked keratocyte proliferation completely.


CONCLUSIONS
MMC treatment regimen, which is common in clinical practice, inhibits keratocyte proliferation during wound healing, but when used at 0.02% for one minute, it does not affect PMN infiltration into the corneal stroma, and subsequent movement toward the injury site, or the disappearance of PMNs from the stroma, in the mouse epithelial injury model.
Escarião
Ana Cecília Souza Leão
AC
Altino Ventura Foundation and Pernambuco Eye Hospital and Columbia University - New York (NY) - USA. ana_cecilia09@yahoo.com
Nagasaki
Takayuki
T
Zhao
Jin
J
Braunstein
Richard
R
D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation / Q000187:drug effects*; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D019573:Epithelium, Corneal / Q000187:drug effects / Q000293:injuries*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology*; D023421:Models, Animal; D009504:Neutrophils / Q000187:drug effects*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D013997:Time Factors
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D001973:Bromodeoxyuridine