Application of physicochemically modified silicon substrates as reverse-phase protein microarrays.
Physicochemically modified silicon substrates can provide a high quality alternative to nitrocellulose-coated glass slides for use in reverse-phase protein microarrays. Enhancement of protein microarray sensitivities is an important goal, especially because molecular targets within patient tissues exist in low abundance. The ideal array substrate has a high protein binding affinity and low intrinsic background signal. Silicon, which has low intrinsic autofluorescence, is being explored as a potential microarray surface. In a previous paper ( Nijdam , A. J. ; Cheng , M. M.-C. ; Fedele , R. ; Geho , D. H. ; Herrmann , P. ; Killian , K. ; Espina , V. ; Petricoin , E. F. ; Liotta , L. A. ; Ferrari , M. Physicochemically Modified Silicon as Substrate for Protein Microarrays . Biomaterials 2007 , 28 , 550 - 558 ), it is shown that physicochemical modification of silicon substrates increases the binding of protein to silicon to a level comparable with that of nitrocellulose. Here, we apply such substrates in a reverse-phase protein microarray setting in two model systems.
Nijdam
A Jasper
AJ
Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA. nijdam@gwu.edu
Zianni
Michael R
MR
Herderick
Edward E
EE
Cheng
Mark M-C
MM
Prosperi
Jenifer R
JR
Robertson
Fredika A
FA
Petricoin
Emanuel F
EF
Liotta
Lance A
LA
Ferrari
Mauro
M
D000418:Albumins / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D000418:Albumins; D012825:Silicon