Characterization of a fission yeast P(5)-type ATPase homologue that is essential for Ca(2+)/Mn(2+ )homeostasis in the absence of P(2)-type ATPases.
In the fission yeast Schizosaccharomyces pombe, three P-type ATPases, namely Cta4p, Pmr1p, and Pmc1p, have been shown to be essential for Ca(2+) homeostasis and are required for specific cellular functions as well. Here, we show that the simultaneous deletion of pmc1(+) and SPAC29A4.19c, which encodes a putative P(5)-type ATPase, causes a hypersensitive growth to either high concentrations of Ca(2+) in a medium, or the antiarrhythmic drug amiodarone, which has been known to cause a disruption of Ca(2+) homeostasis. On the other hand, simultaneous deletion of pmr1(+) and SPAC29A4.19c causes a hypersensitive growth to Mn(2+) depletion in a medium. The green fluorescent protein-tagged SPAC29A4.19c protein reveals a typical localization pattern of the Golgi proteins, but the SPAC29A4.19c protein is not exchangeable in function with Pmr1p, which is required for Ca(2+)/Mn(2+) homeostasis in secretory pathways. These results suggest that the putative P(5)-type ATPase encoded by SPAC29A4.19c is essential for Ca(2+) and Mn(2+ )homeostasis in the absence of P(2)-type ATPases, Pmc1p or Pmr1p, respectively. According to the precedent nomenclature of calcium/cation transporting ATPase in fission yeast, SPAC29A4.19 was named cta5(+) in this study.
Furune
Takahiro
T
Department of Biology, Faculty of Science and Engineering, Konan University, Kobe, Japan.
Hashimoto
Kentaro
K
Ishiguro
Junpei
J
D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D008345:Manganese / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism
D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D008345:Manganese; C539384:Cta5 protein, S pombe; D006180:Proton-Translocating ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium