title: [In vivo effect of annexin I down-regulation on the growth of human pancreatic cancer in nude mice].
abstract: OBJECTIVE
  To further explore the effect of annexin I on the tumor growth of human pancreatic cancer in nude mice.
  
  
  METHODS
  To knock down the expression of annexin I in pancreatic carcinoma cells by RNAi. A nude mouse model of human pancreatic cancer was established by subcutaneous inoculation of human pancreatic cancer cell line Suit-II cells. The effect of annexin I on tumor growth was assessed by tumor growth curve and tumor weight records, and Westen blot and flow cytometry were used to examine the expression of annexin I after annexin I-knocking down.
  
  
  RESULTS
  The results of Western blot revealed that the expression of annexin I was significantly decreased in Suit-II cells transfected with pSilencer-annexin I-siRNA1, and almost completely inhibited in the cells transfected with pSilencer-annexin I-siRNA2 and pSilencer-annexin I-siRNA3. The growth of tumors transfected with annexin I-siRNA2 and annexin I-siRNA3 was inhibited by 76.6% and 68.4%, respectively, in comparison with that of tumor from the parent Suit-II cells. At 44 days after tumor cell inoculation, the tumor weight was 0.8987 g (transfected with annexin I-siRNA2) and 0.8992 g (transfected with annexin I-siRNA3), significantly lower (P < 0.001) than that of tumor from parent Suit-II cells (2.5866 g) and transfected with annexin I-siRNAN (2.4070 g).
  
  
  CONCLUSIONS
  annexin I promotes the growth and proliferation of pancreatic carcinoma cells in vivo and increases the ability of tumor formation in nude mice. The results of this study support that annexin I may become a potential target in gene therapy for this disease.
authors.affiliation: Department of Abdominal Surgical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Ran
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Wang
authors.forename: Dong-Bing
authors.initials: DB
authors.lastname: Zhao
authors.forename: Yan-Tao
authors.initials: YT
authors.lastname: Tian
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Sun
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Xie
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D047368:Tumor Burden
chemical_list: D017305:Annexin A1; D034741:RNA, Small Interfering