[Simultaneous determination of four glycosylflavones from Lophatherum gracile by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for simultaneous determination of orientin, isorientin, vitexin and isovitexin in Lophatherum gracile from different habitat and harvesting time.


METHODS
The HPLC method was applied and the chromatographic column was a Waters XBridge C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase consisted of methanol-0.05% acetic acid (35:65). The flow rate was 1.0 mL min(-1) and the detection wavelength was set at 340 nm. The column temperature was set at 25 degrees C.


RESULTS
Four components were isolated well, the linear relationships were excellent. The mean recoveries and RSD values of orientin, isorientin, vitexin and isovitexin were 103.2%, 2.1%; 101.6%, 2.7%; 98.4%, 2.3%; 99.2%, 1.8%, respectively.


CONCLUSIONS
The HPLC method is simple, sensitive and reliable, and can be used for the quality control of the medicinal material.
Yuan
Ke
K
Research and Development Certer of Natural Medicine, Zhejiang Forestry University, Lin'an 311300, China. yuan_ke001@163.com
Xue
Yue-Qin
YQ
Yin
Ming-Wen
MW
Lou
Lu-Huan
LH
D047310:Apigenin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; D047310:Apigenin; C032731:vitexin; C065886:orientin; C049772:isovitexin