[Expression change of Muscarinic receptor subunits in rat flocculus following unilateral labyrinthectomy].
OBJECTIVE
To observe the expression of Muscarinic receptor M1, M3, M5 subunits in rat flocculus following left unilateral labyrinthectomy (UL).


METHODS
The RT-PCR was used to observe the expression of Muscarinic receptor M1, M3, M5 subunits post-unilateral labyrinthectomy and investigate its effect on vestibular compensation.


RESULTS
Muscarinic receptor M1, M3, M5 subunits were induced decrease in both side flocculus after unilateral labyrinthectomy. The expression was the least in the 1 d flocculus of following UL. The expression is rising from the 3-7 d flocculus of following UL. No difference was observed in the 7 d and sham operation flocculus following UL. No difference was observed in the ipsilateral and contralateral flocculus at any group.


CONCLUSIONS
Muscarinic receptor M1, M3, M5 subunits were induced decrease in the flocculus after unilateral labyrinthectomy. But the significance of the change of Muscarinic receptor M1, M3, M5 subunits in the vestibular compensation is still unknown.
Zou
Yu
Y
Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Kong
Weijia
W
D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D007839:Functional Laterality; D015870:Gene Expression; D008297:Male; D011184:Postoperative Period; D051381:Rats; D043643:Receptor, Muscarinic M1 / Q000378:metabolism*; D043587:Receptor, Muscarinic M3 / Q000378:metabolism*; D043642:Receptor, Muscarinic M5 / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000378:metabolism* / Q000601:surgery
D043643:Receptor, Muscarinic M1; D043587:Receptor, Muscarinic M3; D043642:Receptor, Muscarinic M5