title: Screening for sterilizing activity of antibiotic combinations in an acid model of rapidly growing mycobacteria during the stationary phase of growth.
abstract: BACKGROUND
  In view of the problems of correlation between the data provided by classical microbiological studies and clinical response, we designed an in vitro method to screen for the sterilizing activity of various antibiotics, individually or in combinations, against clinical isolates of various rapidly growing mycobacteria in the stationary phase.
  
  
  METHODS
  We screened a large number of antibiotic combinations (4-16 microg/ml) for their sterilizing capacity in 26 Mycobacterium fortuitum clinical isolates, 7 Mycobacterium chelonae and 2 Mycobacterium abscessus clinical isolates (10(5) CFU).
  
  
  RESULTS
  The best results against M. fortuitum were obtained with moxifloxacin, both on its own (13/26 strains) and in combination. This drug is also very active against M. chelonae (3/7 strains), and in combination with clarithromycin and amikacin exhibits sterilizing activity against all the strains studied. Combinations of clarithromycin with moxifloxacin or linezolid at high doses (16 microg/ml) exhibit activity against M. abscessus.
  
  
  CONCLUSIONS
  The most relevant finding of our study is the good activity of moxifloxacin against these microorganisms in the stationary phase. This indicates the need to confirm these data in animal models or clinical trials in order to determine their true clinical importance.
authors.affiliation: Section of Microbiology, Hospital General Universitario and Universidad Miguel Hernandez, Elche, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Cremades
authors.forename: A
authors.initials: A
authors.lastname: Santos
authors.forename: J C
authors.initials: JC
authors.lastname: Rodríguez
authors.forename: E
authors.initials: E
authors.lastname: García-Pachón
authors.forename: M
authors.initials: M
authors.lastname: Ruiz
authors.forename: I
authors.initials: I
authors.lastname: Escribano
authors.forename: G
authors.initials: G
authors.lastname: Royo
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004338:Drug Combinations; D016926:Mycobacterium chelonae / Q000187:drug effects* / Q000254:growth & development; D019907:Mycobacterium fortuitum / Q000187:drug effects* / Q000254:growth & development; D009170:Nontuberculous Mycobacteria / Q000187:drug effects* / Q000254:growth & development; D013242:Sterilization
chemical_list: D000900:Anti-Bacterial Agents; D004338:Drug Combinations