[Studies on separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins].
OBJECTIVE
To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins.


METHODS
Based on absorptive capacity and elution efficiency of total saponin from flower buds of Panax notoginseng, the best type of resin and the conditions to separate and purify total saponin by macroporous resins were optimized.


RESULTS
The AB-8 resin was chosen, washed with 3 BV of 70% ethanol, and with the absorption and elution velocity of flow 2BV/h. The optimal concentration of the raw material was 0. 2 g/ml. The purity of total saponin obtained was 87.60%.


CONCLUSIONS
Using AB-8 resin to separate and purify total saponin from flowers of Panax notoginseng is feasible.
Wei
Li
L
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. weilitcm@sina.com
Zhou
Hao
H
D000042:Absorption; D000431:Ethanol / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D053723:Panax notoginseng / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
D012117:Resins, Synthetic; D012503:Saponins; D012997:Solvents; D000431:Ethanol