The homeotic protein HOXC13 is a member of human DNA replication complexes.
The homeotic (and oncogenic) HOXC13 protein was shown to have an affinity for a DNA fragment corresponding to the sequence covered by the pre-replicative complex of the human lamin B2 replication origin. We show here that HOXC13 is a member of human replicative complexes. Our fluorescent fusion-protein data demonstrate that it co-localizes with replication foci of early-S cells and that this peculiar behaviour is driven by the homeodomain. By ChIP analysis we also show that HOXC13 binds the lamin B2 replication origin and the origins located near the TOP1 and MCM4 genes in asynchronously growing cells, whereas it does not bind these origins in G(0) resting cells, consistently with its involvement in origin function.
Comelli
Laura
L
Laboratorio di Biologia Molecolare, Scuola Normale Superiore, Pisa, Italy.
Marchetti
Laura
L
Arosio
Daniele
D
Riva
Silvano
S
Abdurashidova
Gulnara
G
Beltram
Fabio
F
Falaschi
Arturo
A
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D004261:DNA Replication; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
C410305:HOXC13 protein, human; D018398:Homeodomain Proteins; D034921:Lamin Type B; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins