title: Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru.
abstract: To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, Lima 31, Peru.
authors.forename: Kristien
authors.initials: K
authors.lastname: Verdonck
authors.forename: Elsa
authors.initials: E
authors.lastname: González
authors.forename: Fernando
authors.initials: F
authors.lastname: Maldonado
authors.forename: Doris
authors.initials: D
authors.lastname: Agapito
authors.forename: Sonia
authors.initials: S
authors.lastname: Van Dooren
authors.forename: Anne-Mieke
authors.initials: AM
authors.lastname: Vandamme
authors.forename: Alfonso
authors.initials: A
authors.lastname: Silva-Santisteban
authors.forename: Guido
authors.initials: G
authors.lastname: Vanham
authors.forename: Daniel
authors.initials: D
authors.lastname: Clark
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gotuzzo
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D015490:HTLV-I Infections / Q000175:diagnosis* / Q000453:epidemiology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D015367:Human T-lymphotropic virus 2 / Q000235:genetics*; D006801:Humans; D008297:Male; D010568:Peru / Q000453:epidemiology; D011237:Predictive Value of Tests; D015995:Prevalence