title: Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes.
abstract: The possibility of obtaining transplantable oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal failures. As reconstructive material this in vitro epithelia would be also useful for other parts of the human body. Many researchers still use controversial methods; therefore it was evaluated and compared the efficiency of the enzymatic and direct explant methods to obtain oral keratinocytes. To this project oral epithelia fragments were used. This work compared: time needed for cell obtainment, best cell amount, life-span and epithelia forming cell capacity. The results showed the possibility to obtain keratinocytes from a small oral fragment and we could verify the advantages and peculiar restrictions. We concluded that under our conditions the enzymatic method showed the best results: in the cells obtaining time needed, cell amount and life-span. Both methods showed the same capacity to form in vitro epithelia.
authors.affiliation: Radiation Technology Center, Nuclear and Energetic Research Institute-IPEN/CNEN, Av. Lineu Prestes 2242, Cidade Universitária, São Paulo, Brazil. fakling@usp.br
authors.forename: Ma Fátima Guarizo
authors.initials: MF
authors.lastname: Klingbeil
authors.forename: Marisa Roma
authors.initials: MR
authors.lastname: Herson
authors.forename: Elier Broche
authors.initials: EB
authors.lastname: Cristo
authors.forename: Décio
authors.initials: D
authors.lastname: dos Santos Pinto
authors.forename: Daniele
authors.initials: D
authors.lastname: Yoshito
authors.forename: Monica Beatriz
authors.initials: MB
authors.lastname: Mathor
mesh_terms: D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology*; D009055:Mouth / Q000737:chemistry / Q000166:cytology*; D009061:Mouth Mucosa / Q000737:chemistry / Q000166:cytology*; D014357:Trypsin / Q000737:chemistry
chemical_list: D014357:Trypsin