[Neuroprotective effects of water extracts of American Ginseng on SH-SY5Y cells apoptosis induced by Abeta25-35].
OBJECTIVE
To study the neuroprotective effect of water extracts of American Ginseng (WEAG) on Abeta25-35-induced SH-SY5Y cells apoptosis in Alzheimer's Disease cellular model.


METHODS
The optimal concentration and treating time of Abeta25-35 for Alzheimer's Disease cellular model as well as those of WEAG were measured by flow cytometer. In addition, the cell viability was measured by MTT test and the morphology of SH-SY5Y cells was observed by Hoechst 33258 staining.


RESULTS
Treated by Abeta25-35 50 micromol/L 72 h later, SH-SY5Y cells turned rounder, aggregated and were positively stained with fluorochrome Hoechst 33258. Cells displayed a typical sub-diploid peak in flow cytometry, and the percentage of apoptosis reaches (37.30 +/- 0.69)% (P < 0.05 as compared with the control group) (1.56 +/- 0.80)%. When incubated with Abeta 50 micromol/L and different doses (0.5, 1, 5 mg/ml) of WEAG for 72h, the characteristics of apoptosis as measured by FCM dose-dependently declined to (16.71 +/- 1.08)%, (10.52 +/- 2.11)% and (3.39 +/- 1.65)%, respectively (P < 0.05 as compared with the model group).


CONCLUSIONS
Water extracts of American Ginseng have markedly neuroprotective effects on SH-SY5Y cells apoptosis induced by Abeta25-35.
Hu
Sheng-Quan
SQ
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen 518057, China. hushengquan@tom.com
Yu
Hui-Min
HM
Liu
Ta-Si
TS
Yang
Da-Jian
DJ
Chen
Xin-Zi
XZ
He
Cui-Jun
CJ
D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D005894:Panax / Q000737:chemistry*; D010446:Peptide Fragments; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35)