The effect of vitamin C on ischemia reperfusion injury because of prolonged tourniquet application with reperfusion intervals.
We examined the effect of vitamin C on muscle injury distal to the tourniquet which was applied for 4 hours with 10- and 20-minute reperfusion intervals after 2 hours of tourniquet. Sixty-four Sprague-Dawley rats were allocated to 4 randomized groups. After 2 hours tourniquet, 10- and 20-minutes of reperfusion were allowed to half of each group respectively. Afterward an additional 2 hours compression was applied. Except the control group the animals received vitamin C intravenously, before the first tourniquet in Group I, at the reperfusion interval in Group II, and at both times in Group III. Malondialdehyde levels were measured in blood and the tibialis anterior muscle. The muscle was histopathologically examined. The data was evaluated statistically. The effects of timing and the dose of vitamin C on ischemia reperfusion injury remain controversial and there was no statistical difference between 10- and 20-minute reperfusion intervals. But the blood malondialdehyde levels showed that vitamin C has a positive effect on the muscle injury caused by ischemia-reperfusion.
Ulug
Burcak Tumerdem
BT
Department of Plastic, Reconstructive, and Aesthetic Surgery, Maltepe University, Istanbul, Turkey. burcaktumerdem@yahoo.com
Aksungar
Fehime Benli
FB
Mete
Ozgur
O
Tekeli
Fatma
F
Mutlu
Nilgun
N
Calik
Burcu
B
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D013997:Time Factors; D014111:Tourniquets
D000975:Antioxidants; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D001205:Ascorbic Acid