[Simultaneous determination of adapalene, 2-phenoxyethanol and methyl-4-hydroxybenzoate in adapalene gels using high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of the contents of adapalene and the preservatives (2-phenoxyethanol and methyl-4-hydroxybenzoate) in adapalene gels. The chromatographic analysis was carried out on a Tigerkin C18 column (150 mm x 4.6 mm, 5 microm). The mobile phase was 0.02 mol/L ammonium acetate buffer (pH 3.0) and tetrahydrofuran-acetonitrile with gradient elution, and the detection wavelength was set at 270 nm. The calibration curves were linear over the ranges of 10-100 mg/L (r = 0.9999), 4-40 mg/L (r = 0.9999) and 4-40 mg/L (r = 0. 9999) for 2-phenoxyethanol, methyl-4-hydroxybenzoate and adapalene, respectively. The average recoveries of the three substances were within 98.0%-98.6%. The method is simple, reliable and suitable for the simultaneous determination of adapalene, 2-phenoxyethanol and methyl-4-hydroxybenzoate in adapalene gels.
Zhang
Chunhong
C
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. zchyan199619@sina.com
Zhao
Yingchun
Y
Han
Chunhui
C
Guo
Xingjie
X
D000068816:Adapalene; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005026:Ethylene Glycols / Q000032:analysis*; D005782:Gels; D057230:Limit of Detection; D016014:Linear Models; D009281:Naphthalenes / Q000032:analysis*; D010226:Parabens / Q000032:analysis*; D015203:Reproducibility of Results; D013997:Time Factors
D005026:Ethylene Glycols; D005782:Gels; D009281:Naphthalenes; D010226:Parabens; D000068816:Adapalene; C015358:methylparaben; C005398:phenoxyethanol