title: Exploration of Csp genes from temperate and glacier soils of the Indian Himalayas and in silico analysis of encoding proteins.
abstract: The metagenomic Csp library was constructed from the temperate and glacier soils of central Himalaya, India followed by polymerase chain reaction (PCR) amplification. The library was further screened for low-temperature adaptation, and the positive recombinants were sorted out by determining changes in the melting temperature (Tm). A homology search of cloned sequence showed their identity with the Csp genes of Pseudomonas fluorescens, Psychrobacter cryohalolentis K5, and Shewanella spp MR-4. Amino acid sequence analysis annotated the presence of conserved aromatic and basic amino acids as well as RNA binding motifs from the cold shock domain. Furthermore, a PROSITE scan showed a moderate identity of less than 60% with the known cold shock-inducible proteins (ribosomal proteins, rbfA, DEAD-box helicases), cold acclimation protein, and temperature-induced protein (SRP1/TIP1). This study highlighted the prevalence of Csp genes from cold Himalayan environments that can be explored for tailor-made crop constructions in future.
authors.affiliation: Department of Microbiology, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, India. premalatha1977@gmail.com
authors.forename: Prema K
authors.initials: PK
authors.lastname: Latha
authors.forename: Ravindra
authors.initials: R
authors.lastname: Soni
authors.forename: Mahejibin
authors.initials: M
authors.lastname: Khan
authors.forename: Soma S
authors.initials: SS
authors.lastname: Marla
authors.forename: Reeta
authors.initials: R
authors.lastname: Goel
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003080:Cold Temperature; D017931:DNA Primers; D005798:Genes, Bacterial; D015698:Genomic Library; D046448:Ice Cover / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D044007:Moraxellaceae / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D017434:Protein Structure, Tertiary; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D020592:Shewanella / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D016601:RNA-Binding Proteins