title: An optimised protocol to prepare Phaeocystis globosa morphotypes for scanning electron microscopy observation.
abstract: A preparation protocol for scanning electron microscopy (SEM) was adapted and tested to the observation of Phaeocystis globosa morphotypes. For extra colonial cells, critical point drying (CPD) gave satisfactory results while for intra colonial cells, preservation with Lugol's iodine and/or glutaraldehyde followed by air-drying appeared as the most suitable method.
authors.affiliation: LOG - UMR CNRS 8187 - Université du Littoral Côte d'Opale, Maison de la Recherche en Environnement Naturel, Wimereux, France. natacha.guiselin@univ-littoral.fr
authors.forename: Natacha
authors.initials: N
authors.lastname: Guiselin
authors.forename: Lucie
authors.initials: L
authors.lastname: Courcot
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Artigas
authors.forename: Aude
authors.initials: A
authors.lastname: Le Jéloux
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Brylinski
mesh_terms: D000818:Animals; D016591:Histocytological Preparation Techniques / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D010839:Phytoplankton / Q000166:cytology* / Q000648:ultrastructure