title: Artificial ribozyme switches containing natural riboswitch aptamer domains.
abstract: RNA Lego: The use of natural riboswitch aptamers in synthetic RNA switches (see picture) should broaden the scope of artificial RNA regulators dramatically. It is shown that thiamine pyrophosphate (TPP) aptamers can be used in engineered devices as very sensitive switches of gene expression in unmodified organisms. The approach demonstrates that intrinsic metabolites can be utilized as external effectors of cellular functions.
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Wieland
authors.forename: Armin
authors.initials: A
authors.lastname: Benz
authors.forename: Benedikt
authors.initials: B
authors.lastname: Klauser
authors.forename: Jörg S
authors.initials: JS
authors.lastname: Hartig
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D015870:Gene Expression; D017930:Genes, Reporter; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000378:metabolism / Q000494:pharmacology
chemical_list: D052157:Aptamers, Nucleotide; D016337:RNA, Catalytic; D013835:Thiamine Pyrophosphate