title: Performance of microcalorimetry for early detection of methicillin resistance in clinical isolates of Staphylococcus aureus.
abstract: We describe a calorimetric assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA) within 5 h. Microbial heat was calculated in culture with and without cefoxitin. Among 30 genetically distinct clinical isolates, 19/20 MRSA (95%) and 10/10 methicillin-susceptible Staphylococcus aureus (100%) were correctly identified. Microcalorimetry may be useful for rapid MRSA screening.
authors.affiliation: Infectious Diseases Research Laboratory, Department of Biomedicine, University Hospital, Basel, Switzerland.
authors.forename: Daniela
authors.initials: D
authors.lastname: Baldoni
authors.forename: Heinz
authors.initials: H
authors.lastname: Hermann
authors.forename: Reno
authors.initials: R
authors.lastname: Frei
authors.forename: Andrej
authors.initials: A
authors.lastname: Trampuz
authors.forename: Andrea
authors.initials: A
authors.lastname: Steinhuber
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002151:Calorimetry / Q000379:methods*; D002440:Cefoxitin / Q000494:pharmacology; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002440:Cefoxitin