Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirus.
The capacity of Lettuce mosaic virus to overcome the lettuce resistance conferred by the mo1(1) and mo1(2) alleles of the gene for eukaryotic translation initiation factor 4E (eIF4E) was analysed using reverse genetics. Mutations in the virus genome-linked protein (VPg) allowed mo1(1) only to be overcome, but mutations in the C-terminal portion of the cylindrical inclusion (CI) protein allowed both alleles to be overcome. Site-directed mutagenesis pinpointed a key role of the amino acid at position 621 in the virulence. This is the first example of the involvement of a potyviral CI protein in the breaking of an eIF4E-mediated resistance.
Abdul-Razzak
Anas
A
Interactions Plante-Virus, UMR GDPP 1090, INRA Université de Bordeaux 2, BP 81, F-33883 Villenave d'Ornon, France.
Guiraud
Thomas
T
Peypelut
Martine
M
Walter
Jocelyne
J
Houvenaghel
Marie-Christine
MC
Candresse
Thierry
T
LE Gall
Olivier
O
German-Retana
Sylvie
S
D001483:Base Sequence; D017931:DNA Primers; D039561:Eukaryotic Initiation Factor-4E / Q000502:physiology*; D018545:Lactuca / Q000821:virology*; D016297:Mutagenesis, Site-Directed; D017800:Potyvirus / Q000378:metabolism* / Q000472:pathogenicity; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D039561:Eukaryotic Initiation Factor-4E; D014764:Viral Proteins; C406275:cylindrical inclusion protein, potyvirus