Molecular diversity of Mycobacterium tuberculosis strains in a slum area of Rio de Janeiro, Brazil.
This retrospective molecular study involving restriction fragment length polymorphism, using insertion sequence 6110 as a marker, was conducted in order to provide an initial insight into the genetic diversity of Mycobacterium tuberculosis strains isolated in the slums of the Complexo de Manguinhos, located in the city of Rio de Janeiro, Brazil. Of the 67 strains evaluated, 23 (34.3%) were found to belong to clusters (total clusters, 10). Household and social chains of transmission were associated with clustering, in 20% and 60%, respectively. Living in the Conjunto Habitacional Programado 2 slum was associated with clustering. Although not significant, it is relevant that 26% of the clustered strains presented primary resistance. These findings, although possibly underestimating the prevalence due to the failure to analyze all strains, could help improve the local tuberculosis control program.
Mendes
Joycenea Matsuda
JM
Centro de Pesquisa Leônidas & Maria Deane, Fundação Instituto Oswaldo Cruz, Manaus, AM, Brazil.
Machado
Silvia Maria Almeida
SM
Lourenço
Maria Cristina
MC
Ferreira
Rosa Maria Carvalho
RM
Fonseca
Leila de Souza
Lde S
Saad
Maria Helena Feres
MH
D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D016000:Cluster Analysis; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D011204:Poverty Areas; D012189:Retrospective Studies; D014376:Tuberculosis / Q000235:genetics* / Q000382:microbiology / Q000635:transmission; D055815:Young Adult
D005819:Genetic Markers