Immunomodulation in the marine gastropod Haliotis diversicolor exposed to benzo(a)pyrene.
It has been reported that environmental pollutants in the aquatic ecosystem could weaken immune competence of organisms. The purpose of the present study was to investigate the effects of benzo(a)pyrene [B(a)P] on immunomodulation in marine gastropods and to see if these effects are caused by or related to the generation of reactive oxygen species. In our present study, the marine gastropod Haliotis diversicolor was exposed to sublethal concentrations (0.01, 0.02, 0.04 and 0.08 mg L(-1)) of B(a)P for 7d under laboratory conditions and the alterations of hematological parameters like haemocyte count, haemocyte viability, protein content and immune components like phenoloxidase, phagocytosis and superoxide anion generation were measured. In addition, the changes in lysozyme activity, antibacterial activity due to the effect of B(a)P on abalone were analysed. B(a)P was found to decrease significantly the total number of circulating haemocytes. Intracellular superoxide anion generation and phenoloxidase significantly increased on exposure to B(a)P, whereas phagocytic activity was decreased significantly at higher concentration. Significant alterations were found in the uptake of neutral red and the observed alterations of hematological parameters and immune components tested indicated the generation of immunotoxicological effects on abalone due to B(a)P exposure. The results demonstrate a possible relationship between B(a)P and the immunological parameters of abalone studied.
Gopalakrishnan
Singaram
S
State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Siming Nanlu 422, Xiamen, Fujian 361005, PR China.
Thilagam
Harikrishnan
H
Huang
Wei-Bin
WB
Wang
Ke-Jian
KJ
D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology / Q000633:toxicity*; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000494:pharmacology / Q000633:toxicity*; D049851:Gastropoda / Q000187:drug effects / Q000276:immunology*; D006434:Hemocytes / Q000187:drug effects; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; D013481:Superoxides; D001564:Benzo(a)pyrene; D014442:Monophenol Monooxygenase