Cytokine expression in colostrum-deprived pigs immunized and challenged with Haemophilus parasuis.
The expression of several cytokines in spleen, pharyngeal lymph nodes, lung and brain after different immunization procedures and a challenge with 5 x 10(9) CFU of Haemophilus parasuis was compared. Five groups of colostrum-deprived pigs were used: vaccinated with (I) a bacterin, (II) an outer-membrane-protein-vaccine, (III) a recombinant transferring-binding protein B, (IV) exposed to a total dose of 10(5) CFU, and (V) not previously immunized. All pigs in groups III and V died, while all animals in group I, most of group IV and half of group II survived until the end of the experiment. IL-1alpha was found in significantly higher levels (p<0.05) in spleen, lymph nodes and brain of dead pigs, which could be explained by the major severity of lesions in these animals. However, IL-4, IL-10, TNF-alpha and IFN-gamma were expressed in significantly higher levels by survivors (for all the four cytokines in lymph nodes; for IL-4, IL-10 and TNF-alpha in spleen; for IL-4, TNF-alpha and IFN-gamma in lung, and only for TNF-alpha in brain), thus suggesting a role of these four cytokines in the adaptive response, which might contribute to protection against H. parasuis infection.
de la Fuente
A J Martín
AJ
Department of Animal Health, Section of Microbiology and Immunology, University of León, 24007-León, Spain.
Ferri
E F Rodríguez
EF
Tejerina
F
F
Frandoloso
R
R
Martínez
S Martínez
SM
Martín
C B Gutiérrez
CB
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D001921:Brain / Q000378:metabolism; D003126:Colostrum; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006192:Haemophilus Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D044137:Haemophilus parasuis; D008168:Lung / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D013154:Spleen / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000378:metabolism
D001428:Bacterial Vaccines; D016207:Cytokines