Protective effect of granulocyte colony-stimulating factor on intracerebral hemorrhage in rat.
Granulocyte colony-stimulating factor (G-CSF) is a member of the cytokine family of growth factors that can protect the neurons from focal cerebral ischemia-induced injuries. The intracerebral hemorrhage (ICH) has been widely observed in the clinic; however, the protective effect of G-CSF on ICH is still elusive. We found in the present study that the intraperitoneal injection of G-CSF for 5 days could improve the ICH-induced neuronal behavioral impairment measured by limb placement assay. We also observed that injection of G-CSF could increase the number of stem cells in the specific zone of the hemorrhagic areas, demonstrated by the enhanced expression of nestin. Additionally, G-CSF could also promote the mobilization of circulating hemopoietic stem cells (HSCs) to the damaged brain areas and activate the astrocytes. Our results reveal that G-CSF is also protective for the ICH with the mechanisms involving proliferation of neural stem cells, the migration of HSCs and the activation of astrocytes.
Zhang
Lei
L
Hubei Provincial Key Laboratory of Neurological Disease, Pathophysiology Department, Tongji Medical College, Hua-Zhong University of Science and Technology, 430030, Wuhan, People's Republic of China.
Shu
Xi-Ji
XJ
Zhou
Hong-Yan
HY
Liu
Wei
W
Chen
Ying
Y
Wang
Cui-Lan
CL
Li
Yan
Y
Chen
Qiong-Xia
QX
Liu
Li-Jiang
LJ
Wang
Jian-Zhi
JZ
D000818:Animals; D001253:Astrocytes / Q000166:cytology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002543:Cerebral Hemorrhage / Q000188:drug therapy*; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064231:Nestin; D011596:Psychomotor Disorders / Q000188:drug therapy; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor