title: Chikungunya virus isolated in Lakshadweep islands in the Indian Ocean: evidence of the Central/East African genotype.
abstract: Virological investigation was carried out to determine the etiology of suspected Chikungunya fever among humans reported in the Lakshadweep islands in the Indian Ocean. Three out of 23 acute sera samples showed cytopathological changes in Vero cell lines. Further, indirect immunofluorescence antibody test and reverse transcriptase-polymerase chain reaction studies demonstrated the Chikungunya virus etiology during the episode. E1 gene sequence analysis has confirmed the involvement of the Central/East African genotype of the Chikungunya virus.
authors.affiliation: Centre for Research in Medical Entomology, Madurai, India. crmeicmr@icmr.org
authors.forename: Rajaiah
authors.initials: R
authors.lastname: Paramasivan
authors.forename: Paulraj
authors.initials: P
authors.lastname: Philip Samuel
authors.forename: Velayutham
authors.initials: V
authors.lastname: Thenmozhi
authors.forename: Rathinasamy
authors.initials: R
authors.lastname: Rajendran
authors.forename: Soosaimanickam
authors.initials: S
authors.lastname: Victor Jerald Leo
authors.forename: Kutty Jagadeeswaran
authors.initials: KJ
authors.lastname: Dhananjeyan
authors.forename: Ranganathan
authors.initials: R
authors.lastname: Krishnamoorthi
authors.forename: Natarajan
authors.initials: N
authors.lastname: Arunachalam
authors.forename: Brij Kishore
authors.initials: BK
authors.lastname: Tyagi
mesh_terms: D000350:Africa, Central; D000351:Africa, Eastern; D018354:Alphavirus Infections / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D006801:Humans; D019156:Indian Ocean; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis
chemical_list: D000914:Antibodies, Viral