Modulation of steroidogenic enzymes in murine lymphoid organs after immune activation.
To study the effects of immune cell activation by a protein antigen or lymphoid tissue derived cytokines on peripheral steroidogenesis activities of 3beta HSD and 17beta HSD was measured in lymphoid organs of control and BSA immunized mice after 3 weeks treatment. We demonstrated the presence of 3betaHSD and 17betaHSD in the lymphoid organs after active immunization. We found elevated serum corticosterone after 3 weeks of antigen administration in presence of CFA and a higher serum IL-6 level that also alter lymphoid tissue cytokine responses like TNF-alpha, IL-12p70, and IL-6, among which IL-12p70 and TNF-alpha down-regulate the activity of steroidogenic enzymes in the thymus during an immune response.
Mukhopadhyay
Rupanjan
R
Department of Physiology, University Colleges of Science and Technology, University of Calcutta, West Bengal, India.
Mishra
Manoj K
MK
Basu
Anirban
A
Bishayi
Biswadev
B
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003345:Corticosterone / Q000097:blood*; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004960:Estradiol Dehydrogenases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005620:Freund's Adjuvant; D015971:Gene Expression Regulation, Enzymologic; D007109:Immunity; D007114:Immunization; D008213:Lymphocyte Activation / Q000235:genetics; D008221:Lymphoid Tissue / Q000201:enzymology* / Q000276:immunology; D008297:Male; D051379:Mice; D009928:Organ Specificity; D012710:Serum Albumin, Bovine; D015533:Transcriptional Activation / Q000276:immunology
D016207:Cytokines; D012710:Serum Albumin, Bovine; D005620:Freund's Adjuvant; D015096:3-Hydroxysteroid Dehydrogenases; C511890:3beta-hydroxysteroid dehydrogenase 4, mouse; D004960:Estradiol Dehydrogenases; D003345:Corticosterone