title: Effects of replacement of low-spin haem b by haem O on Escherichia coli cytochromes bo and bd quinol oxidases.
abstract: Cytochromes bo and bd are terminal ubiquinol oxidases in the aerobic respiratory chain of Escherichia coli and generate proton motive force across the membrane. To probe roles of haem species in the oxidation of quinols, intramolecular electron transfer and the dioxygen reduction, we replaced b-haems with haem O by using the haem O synthase-overproducing system, which can accumulate haem O in cytoplasmic membranes. Characterizations of spectroscopic properties of cytochromes bo and bd isolated from BL21 (DE3)/pLysS and BL21 (DE3)/pLysS/pTTQ18-cyoE after 4 h of the aerobic induction of haem O synthase (CyoE) showed the specific incorporation of haem O into the low-spin haem-binding site in both oxidases. We found that the resultant haem oo- and obd-type oxidase severely reduced the ubiquinol-1 oxidase activity due to the perturbations of the quinol oxidation site. Our observations suggest that haem B is required at the low-spin haem site for the oxidation of quinols by cytochromes bo and bd.
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo, Japan. tmogi@m.u-tokyo.ac.jp
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Mogi
mesh_terms: D002852:Chromatography, Ion Exchange; D003573:Cytochrome b Group / Q000378:metabolism*; D003580:Cytochromes / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006418:Heme / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D015363:Quinolones / Q000378:metabolism; D013057:Spectrum Analysis
chemical_list: D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D015363:Quinolones; C052097:aurachin D; C052096:aurachin C; C069808:heme O; D006418:Heme; C017168:cytochrome bo, E coli; D010088:Oxidoreductases; C040077:cytochrome bd terminal oxidase complex, E coli