title: STD sensor based on nucleic acid functionalized nanostructured polyaniline.
abstract: STD (sexually transmitted disease, Gonorrhoea) sensor based on nucleic acid probe (from Opa, a multi-copy gene of Neisseria gonorrhoeae) functionalized nanostructured-polyaniline coated onto indium-tin-oxide-coated glass plate has been fabricated using avidin-biotin as cross-linking agent. This DNA functionalized electrode can specifically detect upto 0.5 x 10(-15)M of complementary target within 60s of hybridization time at 25 degrees C by differential pulse voltammetry (DPV) using methylene blue as electro-active DNA hybridization indicator. This highly sensitive and specific nucleic acid functionalized nanostructured-polyaniline electrode can distinguish presence of N. gonorrhoeae from Neisseria meningitidis and Escherichia coli culture and spiked samples from the urethral swabs of the patients.
authors.affiliation: Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.forename: Rachna
authors.initials: R
authors.lastname: Prasad
authors.forename: G
authors.initials: G
authors.lastname: Sumana
authors.forename: Kavita
authors.initials: K
authors.lastname: Arora
authors.forename: Seema
authors.initials: S
authors.lastname: Sood
authors.forename: R K
authors.initials: RK
authors.lastname: Gupta
authors.forename: B D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D015169:Colony Count, Microbial / Q000295:instrumentation; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D008297:Male; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000814:Aniline Compounds; D004269:DNA, Bacterial; C416807:polyaniline