title: Bone marrow stromal cells attenuate injury-induced changes in galanin, NPY and NPY Y1-receptor expression after a sciatic nerve constriction.
abstract: Single ligature nerve constriction (SLNC) of the rat sciatic nerve triggers neuropathic pain-related behaviors and induces changes in neuropeptide expression in primary afferent neurons. Bone marrow stromal cells (MSCs) injected into the lumbar 4 (L4) dorsal root ganglia (DRGs) of animals subjected to a sciatic nerve SLNC selectively migrate to the other ipsilateral lumbar DRGs (L3, L5 and L6) and prevent mechanical and thermal allodynia. In this study, we have evaluated the effect of MSC administration on the expression of the neuropeptides galanin and NPY, as well as the NPY Y(1)-receptor (Y(1)R) in DRG neurons. Animals were subjected to a sciatic nerve SLNC either alone or followed by the administration of MSCs, phosphate-buffered saline (PBS) or bone marrow non-adherent mononuclear cells (BNMCs), directly into the ipsilateral L4 DRG. Seven days after injury, the ipsilateral and contralateral L4-5 DRGs were dissected out and processed for standard immunohistochemistry, using specific antibodies. As previously reported, SLNC induced an ipsilateral increase in the number of galanin and NPY immunoreactive neurons and a decrease in Y(1)R-positive DRG neurons. The intraganglionic injection of PBS or BNMCs did not modify this pattern of expression. In contrast, MSC administration partially prevented the injury-induced changes in galanin, NPY and Y(1)R expression. The large number of Y(1)R-immunoreactive neurons together with high levels of NPY expression in animals injected with MSCs could explain, at least in part, the analgesic effects exerted by these cells. Our results support MSC participation in the modulation of neuropathic pain and give insight into one of the possible mechanisms involved.
authors.affiliation: School of Biomedical Sciences, Austral University, Buenos Aires, Argentina. fcoronel@cas.austral.edu.ar
authors.forename: M F
authors.initials: MF
authors.lastname: Coronel
authors.forename: P L
authors.initials: PL
authors.lastname: Musolino
authors.forename: P R
authors.initials: PR
authors.lastname: Brumovsky
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
authors.forename: M J
authors.initials: MJ
authors.lastname: Villar
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D003251:Constriction, Pathologic / Q000378:metabolism; D019004:Galanin / Q000096:biosynthesis*; D009478:Neuropeptide Y / Q000096:biosynthesis*; D051381:Rats; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis*; D012584:Sciatic Nerve / Q000293:injuries*; D020426:Sciatic Neuropathy; D033581:Stem Cell Transplantation; D017154:Stromal Cells / Q000502:physiology*; D016896:Treatment Outcome; D014947:Wounds and Injuries / Q000378:metabolism
chemical_list: D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; D019004:Galanin