[Expression of P-selectin in the penile vascular epithelia of smoking rats].
OBJECTIVE
To observe the expression of P-selectin in the penile vascular epithelial cells and the morphological changes in the ultrastructure of the penile cavernous tissues of smoking rats, and to explore the pathogenesis of smoking-induced erectile dysfunction.


METHODS
Fifty healthy Wistar rats were randomly divided into a normal control, a long-term heavy smoking group, a long-term light smoking, a short-term heavy smoking and a smoking cessation group. Their erectile function was tested by subcutaneous injection of apomorphine (APO), the P-selectin expression in the penile vascular epithelial cells detected by ELISA and the morphological changes in the ultrastructure of the penile cavernous tissues observed under the transmission electron microscope (TEM).


RESULTS
The levels of P-selectin were 10.78 +/- 1.71 ng/L, 62.62 +/- 5.95 ng/L, 40.06 +/- 3.97 ng/L, 41.37 +/- 4.06 ng/L and 22.80 +/- 3.15 ng/L respectively in the normal control, long-term heavy smoking, long-term light smoking, short-term heavy smoking and smoking cessation groups, with significant differences between the control group and the other four (P < 0.05). Electron microscopy showed abnormal arrangement of endothelia, penile cavernous sinuses and smooth muscle cells, disrupted continuity of endothelia, damaged ultrastructure of endothelial and smooth muscle cells in the penile cavernous tissue, and obvious proliferation and fibrosis of interstitial tissues in the smoking rats.


CONCLUSIONS
Smoking increases the P-selectin expression in the penile vascular epithelial cells and damages the ultrastructure of the penile cavernous tissue, which may be the main contributors to smoking-induced erectile dysfunction.
Yang
Hua
H
Department of Urology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China. yanghuazyl@163.com
Wang
Dong-Wen
DW
Shuang
Wei-Bing
WB
Wang
Jing-Qi
JQ
Zhang
Li
L
Zhao
Bin
B
Zhang
Jian
J
D000818:Animals; D004848:Epithelium / Q000378:metabolism; D008297:Male; D019007:P-Selectin / Q000096:biosynthesis*; D010410:Penile Erection; D010413:Penis / Q000098:blood supply / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*
D019007:P-Selectin