Functional involvement of RINF, retinoid-inducible nuclear factor (CXXC5), in normal and tumoral human myelopoiesis.
Retinoids triggers differentiation of acute promyelocytic leukemia (APL) blasts by transcriptional regulation of myeloid regulatory genes. Using a microarray approach, we have identified a novel retinoid-responsive gene (CXXC5) encoding a nuclear factor, retinoid-inducible nuclear factor (RINF), that contains a CXXC-type zinc-finger motif. RINF expression correlates with retinoid-induced differentiation of leukemic cells and with cytokine-induced myelopoiesis of normal CD34(+) progenitors. Furthermore, short hairpin RNA (shRNA) interference suggests for this gene a regulatory function in both normal and tumoral myelopoiesis. Interestingly, RINF localizes to 5q31.3, a small region often deleted in myeloid leukemia (acute myeloid leukemia [AML]/myelodysplasia [MDS]) and suspected to harbor one or several tumor suppressor gene.
Pendino
Frédéric
F
Department of Molecular Biology, University of Bergen, Bergen, Norway.
Nguyen
Eric
E
Jonassen
Inge
I
Dysvik
Bjarte
B
Azouz
Abdulkader
A
Lanotte
Michel
M
Ségal-Bendirdjian
Evelyne
E
Lillehaug
Johan R
JR
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D042381:Granulocyte Precursor Cells / Q000187:drug effects / Q000502:physiology; D018922:HL-60 Cells; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology*; D020014:K562 Cells; D008954:Models, Biological; D008969:Molecular Sequence Data; D038042:Myelopoiesis / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C540448:CXXC5 protein, human; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D014157:Transcription Factors; D014212:Tretinoin