Transcript profiling of oilseed rape (Brassica napus) primed for biocontrol differentiate genes involved in microbial interactions with beneficial Bacillus amyloliquefaciens from pathogenic Botrytis cinerea.
Many microorganisms interact with plants but information is insufficient concerning requirements for plant colonization and if interactions become beneficial or detrimental. Pretreatment of oilseed rape (Brassica napus) with Bacillus results in disease suppression upon challenge with pathogens. We have studied transcriptome effects on oilseed rape primed with the Bacillus amyloliquefaciens 5113 biocontrol strain and compared that with effects of the fungal pathogen Botrytis cinerea. Using the cDNA-AFLP technique 21,700 transcript fragments were obtained of which 120 were differentially expressed and verified by northern blot analysis for selected transcripts. Priming with Bacillus caused greater effect on leaf than root transcripts where sequencing and BLAST analysis suggested many of the transcripts to be involved in metabolism and bioenergy. Bacillus and Botrytis treatment also changed metabolic gene expression in addition to signaling and transcription control genes as well as a potential disease resistance (TIR-NBS-LRR) gene. The pathogen provoked non-primed plant profile was less dominated by metabolism than Bacillus and Bacillus-Botrytis treated plants. Several transcripts were homologues to unknown genes in the different treatments. Altogether Bacillus treatment of roots cause a systemic gene expression in leaves suggested to result in a metabolic reprogramming as a major event during priming.
Sarosh
Bejai R
BR
Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7080, 750 07, Uppsala, Sweden. bejai.sarosh@vbsg.slu.se
Danielsson
Jesper
J
Meijer
Johan
J
D054458:Amplified Fragment Length Polymorphism Analysis; D000898:Antibiosis / Q000235:genetics*; D001407:Bacillus / Q000254:growth & development*; D020171:Botrytis / Q000472:pathogenicity*; D029688:Brassica napus / Q000235:genetics* / Q000382:microbiology; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010935:Plant Diseases / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D018744:DNA, Plant