H-type bovine spongiform encephalopathy: complex molecular features and similarities with human prion diseases.
We previously reported that some cattle affected by bovine spongiform encephalopathy (BSE) showed distinct molecular features of the protease-resistant prion protein (PrP(res)) in Western blot, with a 1-2 kDa higher apparent molecular mass of the unglycosylated PrP(res) associated with labelling by antibodies against the 86-107 region of the bovine PrP protein (H-type BSE). By Western blot analyses of PrP(res), we now showed that the essential features initially described in cattle were observed with a panel of different antibodies and were maintained after transmission of the disease in C57Bl/6 mice. In addition, antibodies against the C-terminal region of PrP revealed a second, more C-terminally cleaved, form of PrP(res) (PrP(res) #2), which, in unglycosylated form, migrated as a approximately 14 kDa fragment. Furthermore, a PrP(res) fragment of approximately 7 kDa, which was not labelled by C-terminus-specific antibodies and was thus presumed to be a product of cleavage at both N- and C-terminal sides of PrP protein, was also detected. Both PrP(res) #2 and approximately 7 kDa PrP(res) were detected in cattle and in C57Bl/6 infected mice. These complex molecular features are reminiscent of findings reported in human prion diseases. This raises questions regarding the respective origins and pathogenic mechanisms in prion diseases of animals and humans.
Biacabe
Anne-Gaëlle
AG
AFSSA-Lyon, Unit ATNC, Lyon, France.
Jacobs
Jorg G
JG
Bencsik
Anna
A
Langeveld
Jan P M
JP
Baron
Thierry G M
TG
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000276:immunology* / Q000378:metabolism / Q000635:transmission; D005260:Female; D006801:Humans; D051379:Mice; D018620:PrPSc Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D017434:Protein Structure, Tertiary
D000906:Antibodies; D018620:PrPSc Proteins