Effects of 17beta-estradiol, and its metabolite, 4-hydroxyestradiol on fertilization, embryo development and oxidative DNA damage in sand dollar (Dendraster excentricus) sperm.
Oxidative compounds have been demonstrated to decrease the fertilization capability and viability of offspring of treated spermatozoa. As estrogen and its hydroxylated metabolites readily undergo redox cycling, this study was undertaken to determine if estrogens and other oxidants could damage DNA and impair sperm function. Sperm was preexposed to either 17beta-estradiol (E2), 4-hydroxyestradiol (4OHE2) or the oxidant t-butyl hydroperoxide (t-BOOH), and allowed to fertilize untreated eggs. The fertilization rates and development of the larvae were assessed, as well as the amount of 8-oxodeoxyguanosine (8-oxodG) as an indication of oxidative DNA damage. All compounds caused significant decreases in fertilization and increases in pathological abnormalities in offspring, with 4OHE2 being the most toxic. Treatment with 4OHE2 caused a significant increase of 8-oxodG, but E2 failed to show any effect. Pathological abnormalities were significantly correlated (r(2)=0.44, p< or =0.05) with 8-oxodG levels in sperm treated with t-BOOH and 4OHE2, but not E2. 8-OxodG levels also were somewhat weakly correlated with impaired fertilization in 4OHE2-treated sperm (r(2)=0.33, p< or =0.05). The results indicate that biotransformation of E2 to 4OHE2 enhances oxidative damage of DNA in sperm, which can reduce fertilization and impair embryonic development, but other mechanisms of action may also contribute to these effects.
Rempel
Mary Ann
MA
Environmental Toxicology Program, University of California Riverside, Riverside, CA, United States.
Hester
Brian
B
Deharo
Hector
H
Hong
Haizheng
H
Wang
Yinsheng
Y
Schlenk
Daniel
D
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000633:toxicity*; D004967:Estrogens / Q000633:toxicity*; D002393:Estrogens, Catechol; D005306:Fertilization / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D012617:Sea Urchins / Q000187:drug effects* / Q000196:embryology / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects*; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D004967:Estrogens; D002393:Estrogens, Catechol; D004958:Estradiol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D020122:tert-Butylhydroperoxide; C014036:4-hydroxyestradiol; D003849:Deoxyguanosine