Maturation of the translation inhibitor microcin C.
Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibition of the McC target, aspartyl-tRNA synthetase.
Metlitskaya
Anastasia
A
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Kazakov
Teymur
T
Vondenhoff
Gaston H
GH
Novikova
Maria
M
Shashkov
Alexander
A
Zatsepin
Timofei
T
Semenova
Ekaterina
E
Zaitseva
Natalia
N
Ramensky
Vasily
V
Van Aerschot
Arthur
A
Severinov
Konstantin
K
D001226:Aspartate-tRNA Ligase / Q000037:antagonists & inhibitors / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D053898:Biosynthetic Pathways; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008958:Models, Molecular; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D001430:Bacteriocins; D029968:Escherichia coli Proteins; D011500:Protein Synthesis Inhibitors; C020411:microcin; D001226:Aspartate-tRNA Ligase