Grafts of porcine small intestinal submucosa with cultured autologous oral mucosal epithelial cells for esophageal repair in a canine model.
The acellular porcine small intestinal submucosa (SIS) has been successfully used for esophagoplasty. However, it does not lead to a complete epithelialization in a canine model. A cellular component may be required for better reconstruction. The present study was undertaken to investigate the feasibility and effectiveness of the combination of SIS and autologous oral mucosal epithelial cells (OMECs) for esophageal reconstruction. The OMECs harvested from beagle dogs were cultured and propagated, and the 3rd passage cells were seeded on a single-layer SIS. Male beagle dogs were subjected to surgical resection to produce cervical esophageal defects (5 cm in length, 180 degrees in range). SIS with or without OMECs was patched on the esophageal defects. Barium esophagram, immunohistochemistry, and histology were performed to evaluate the therapeutic effectiveness. Four weeks after surgery, the histological examination showed a complete re-epithelialization and almost no inflammation in the SIS with OMECs group. But in the SIS group, only a partial epithelialization was observed along with inflammation. Eight weeks after surgery, the squamous epithelium was found to cover the entire graft surface in both groups; however, the muscular regeneration was observed in the SIS with OMECs, but not in the SIS group. The graft of SIS combined with autologous OMECs promotes re-epithelialization and muscular regeneration. It is an effective alternative method for esophageal repair.
Wei
Ren-Qian
RQ
Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China.
Tan
Bo
B
Tan
Mei-Yun
MY
Luo
Jing-Cong
JC
Deng
Li
L
Chen
Xiao-He
XH
Li
Xiu-Qun
XQ
Zuo
Xiao
X
Zhi
Wei
W
Yang
Peng
P
Xie
Hui-Qi
HQ
Yang
Zhi-Ming
ZM
D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D004285:Dogs; D004944:Esophagoplasty / Q000379:methods*; D004947:Esophagus / Q000473:pathology / Q000601:surgery; D007413:Intestinal Mucosa / Q000637:transplantation*; D007421:Intestine, Small / Q000637:transplantation; D009061:Mouth Mucosa / Q000166:cytology* / Q000637:transplantation; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous