title: Maternal MTHFR polymorphisms and risk of spontaneous abortion.
abstract: OBJECTIVE
  To asses the association between intake of folate and B vitamins and the incidence of spontaneous abortion (SA) according to the maternal methylenetetrahydrofolate reductase (MTHFR) polymorphisms (677 C>T and 1298 A>C).
  
  
  METHODS
  We conducted a nested case-control study within a perinatal cohort of women recruited in the state of Morelos, Mexico. Twenty-three women with SA were compared to 74 women whose pregnancy survived beyond week 20th. Intake of folate and B vitamins respectively, was estimated using a validated food frequency questionnaire. Maternal MTHFR polymorphisms were determined by PCR-RFLP and serum homocysteine levels by HPLC.
  
  
  RESULTS
  Carriers of MTHFR 677TT and 1298AC genotypes respectively showed an increased risk of SA (OR 677TT vs. CC/CT=5.0; 95% CI: 1.2, 20.9 and OR 1298 AC vs. AA=5.5; 95% CI: 1.1, 26.6).
  
  
  CONCLUSIONS
  Our results support the role of MTHFR polymorphisms as a risk factor for SA, regardless of dietary intake of B vitamins.
authors.affiliation: National Institute of Public Health, Morelos, México.
authors.forename: María del Rosario
authors.initials: Mdel R
authors.lastname: Rodríguez-Guillén
authors.forename: Luisa
authors.initials: L
authors.lastname: Torres-Sánchez
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Marcia
authors.initials: M
authors.lastname: Galván-Portillo
authors.forename: Julia
authors.initials: J
authors.lastname: Blanco-Muñoz
authors.forename: Miriam Aracely
authors.initials: MA
authors.lastname: Anaya
authors.forename: Irma
authors.initials: I
authors.lastname: Silva-Zolezzi
authors.forename: María A
authors.initials: MA
authors.lastname: Hernández-Valero
authors.forename: Lizbeth
authors.initials: L
authors.lastname: López-Carrillo
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology / Q000235:genetics*; D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D004034:Diet Surveys; D005260:Female; D005492:Folic Acid; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008800:Mexico / Q000453:epidemiology; D009790:Occupations / Q000706:statistics & numerical data; D018812:Paternal Exposure / Q000706:statistics & numerical data; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D012307:Risk Factors; D012907:Smoking / Q000453:epidemiology; D014803:Vitamin B Complex; D014931:Women, Working; D055815:Young Adult
chemical_list: D006710:Homocysteine; D014803:Vitamin B Complex; D005492:Folic Acid; D042965:Methylenetetrahydrofolate Reductase (NADPH2)