title: Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy.
abstract: Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers. Furthermore, collagen orientation in post-UVB-exposed skin with few wrinkles changed from that of UVB-exposed wrinkled skin to that of no-UVB-exposed skin. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging.
authors.affiliation: Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan. t-yasui@me.es.osaka-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasui
authors.forename: Yu
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Fukushima
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogura
authors.forename: Toyonobu
authors.initials: T
authors.lastname: Yamashita
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kuwahara
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Hirao
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
mesh_terms: D000818:Animals; D020405:Dermis / Q000502:physiology* / Q000528:radiation effects; D024022:Fibrillar Collagens / Q000378:metabolism*; D051379:Mice; D008859:Microscopy, Polarization / Q000379:methods*; D015595:Skin Aging / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D024022:Fibrillar Collagens