title: Simultaneous determination of olmesartan medoxomil and irbesartan and hydrochlorothiazide in pharmaceutical formulations and human serum using high performance liquid chromatography.
abstract: A simple, selective, sensitive, precise, simultaneous high performance liquid chromatographic analysis of serum samples and commercial tablet formulation containing hydrochlorothiazide, olmesartan medoxomil and irbesartan are reported. Good chromatographic separation was achieved using a micro-Bondapak, C18 column (15 cm x 4.6 mm, 5 microm), and a mobile phase consisting of acetonitrile-0.2% acetic acid aqueous solution (50:50, v/v) at a flow rate of 1.0 mL/min. The ultraviolet detector was set at a wavelength of 260 nm. Hydrochlorothiazide, olmesartan medoxomil, and irbesartan were eluted at 1.2, 3.8, and 4.4 min, respectively. No extraneous materials were found to interfere. The method uses protein precipitation with acetonitrile for the preparation of serum sample. The linear ranges for hydrochlorothiazide, olmesartan medoxomil, and irbesartan were 6.25-18.75, 20-60, and 75-225 ng/mL, respectively. The recoveries of hydrochlorothiazide, olmesartan medoxomil, and irbesartan in spiked samples were all greater than 98%, and their relative standard deviations were less than 2.0%. The limits of detection were 1, 2, and 2 ng/mL for hydrochlorothiazide, olmesartan medoxomil, and irbesartan, respectively, and the limits of quantification were 3 ng/mL, which allow their determination at the expected serum concentration levels.
authors.affiliation: Department of Pharmaceutical Chemistry, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan. msarayne@gmail.com
authors.forename: Najma
authors.initials: N
authors.lastname: Sultana
authors.forename: M Saeed
authors.initials: MS
authors.lastname: Arayne
authors.forename: S Shahid
authors.initials: SS
authors.lastname: Ali
authors.forename: Shahnawaz
authors.initials: S
authors.lastname: Sajid
mesh_terms: D001713:Biphenyl Compounds / Q000032:analysis* / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006852:Hydrochlorothiazide / Q000032:analysis* / Q000097:blood; D007093:Imidazoles / Q000032:analysis* / Q000097:blood; D000077405:Irbesartan; D000068557:Olmesartan Medoxomil; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013777:Tetrazoles / Q000032:analysis* / Q000097:blood; D013997:Time Factors
chemical_list: D001713:Biphenyl Compounds; D007093:Imidazoles; D004364:Pharmaceutical Preparations; D013777:Tetrazoles; D006852:Hydrochlorothiazide; D000068557:Olmesartan Medoxomil; D000077405:Irbesartan