Efficient gene delivery to the adult and fetal CNS using pseudotyped non-integrating lentiviral vectors.
Non-integrating lentiviral vectors show considerable promise for gene therapy applications as they persist as long-term episomes in non-dividing cells and diminish risks of insertional mutagenesis. In this study, non-integrating lentiviral vectors were evaluated for their use in the adult and fetal central nervous system of rodents. Vectors differentially pseudotyped with vesicular stomatitis virus, rabies and baculoviral envelope proteins allowed targeting of varied cell populations. Efficient gene delivery to discrete areas of the brain and spinal cord was observed following stereotactic administration. Furthermore, after direct in utero administration (E14), sustained and strong expression was observed 4 months into adulthood. Quantification of transduction and viral copy number was comparable when using non-integrating lentivirus and conventional integrating vector. These data support the use of non-integrating lentiviral vectors as an effective alternative to their integrating counterparts in gene therapy applications, and highlight their potential for treatment of inherited and acquired neurological disorders.
Rahim
A A
AA
Perinatal Brain Protection and Repair Group, Department of Obstetrics and Gynaecology, University College London, London, UK.
Wong
A M S
AM
Howe
S J
SJ
Buckley
S M K
SM
Acosta-Saltos
A D
AD
Elston
K E
KE
Ward
N J
NJ
Philpott
N J
NJ
Cooper
J D
JD
Anderson
P N
PN
Waddington
S N
SN
Thrasher
A J
AJ
Raivich
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism; D046128:Fetal Therapies / Q000379:methods; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage*; D016086:Lentivirus / Q000235:genetics* / Q000502:physiology; D051379:Mice; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism; D013238:Stereotaxic Techniques; D014161:Transduction, Genetic; D016662:Virus Integration