title: The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat.
abstract: As part of an ongoing research program dedicated to the understanding of proanthocyanidin (PA) accumulation in Brassica napus seed coat, transgenic rapeseed plants carrying a 2.3-kb fragment of the Arabidopsis thaliana BAN promoter (ProAtBAN) fused to the uidA reporter gene (GUS) were generated. Analysis of these plants revealed that ProAtBAN was activated in B. napus seed coat, following a spatio-temporal pattern that was very similar to the PA deposition profile in rapeseed and also to the one previously described in Arabidopsis. ProAtBAN activity occurred as soon as the early stages of embryogenesis and was restricted to the cells where PAs were shown to accumulate. Therefore, the Arabidopsis BAN promoter can be used to trigger gene expression in B. napus seed coat for both genetic engineering and functional validation of candidate genes. In addition, these data strongly suggest that the transcriptional regulatory network of the BAN gene is conserved between Arabidopsis and rapeseed. This is consistent with the fact that similarity searches of the public rapeseed sequence databases allowed recovering the rapeseed homologs for several BAN regulators, namely TT1, TT2, TT8, TT16 and TTG1, which have been previously described in Arabidopsis.
authors.affiliation: UMR118 INRA-Agrocampus Ouest-Université de Rennes1 Amélioration des Plantes et Biotechnologies Végétales, Le Rheu Cedex, France. nathalie.nesi@rennes.inra.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nesi
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Lucas
authors.forename: Bathilde
authors.initials: B
authors.lastname: Auger
authors.forename: Cécile
authors.initials: C
authors.lastname: Baron
authors.forename: Alain
authors.initials: A
authors.lastname: Lécureuil
authors.forename: Philippe
authors.initials: P
authors.lastname: Guerche
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Kronenberger
authors.forename: Loïc
authors.initials: L
authors.lastname: Lepiniec
authors.forename: Isabelle
authors.initials: I
authors.lastname: Debeaujon
authors.forename: Michel
authors.initials: M
authors.lastname: Renard
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D029688:Brassica napus / Q000235:genetics / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D044945:Proanthocyanidins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D044945:Proanthocyanidins; C013221:proanthocyanidin; C401411:BANYULS protein, Arabidopsis; D009247:NADH, NADPH Oxidoreductases