title: Proteomic analysis of normal human nasal mucosa: establishment of a two-dimensional electrophoresis reference map.
abstract: OBJECTIVE
  To construct a partial two-dimensional electrophoresis (2-DE) reference map of the proteins that compose normal human nasal mucosa.
  
  
  METHODS
  Normal inferior turbinate mucosa samples were subjected to 2-DE, the protein spots were visualized by silver staining, and 78 spots were selected for analysis by mass spectrometry and bioinformatics. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical techniques were performed for validation and localization analysis.
  
  
  RESULTS
  Among the identified proteins, the largest functional groups included proteins associated with the human immune response and enzymes, particularly those of protein metabolism. Proteins participating in the cell cycle, cell division, calcium metabolism, and ion transport were also detected. The mRNA transcripts for 10 selected proteins were amplified by RT-PCR. Immunohistochemistry revealed that secretagogin was localized in the submucosal gland and calsenilin was localized in the epithelium and submucosal gland.
  
  
  CONCLUSIONS
  This database will serve as the basis for further comparative proteomic studies of nasal mucosal disorders.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Soonchunhyang University College of Medicine, Bucheon, South Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jang Yul
authors.initials: JY
authors.lastname: Byun
authors.forename: Sang Hag
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult