Ultradense synthetic gene brushes on a chip.
Dense brushes of linear DNA polymers are assembled on a biochip with approximately 30 nm between anchorage points, amounting to a few mega-base-pairs/microm(3). In bulk solution, a barrier incurs to conjugate more than two end-functionalized DNAs. However, such doublets bind the surface with almost equal efficiency to singlets, suggesting that extended brush buildup reduces the barrier. On-chip transcription reveals that doublets are roughly 2-fold inefficient compared to singlets, a manifestation of the interaction of the enzymatic machinery with the dense brush. Synthetic gene brushes made of DNA conjugates provide simple means to regulate expression on a chip.
Buxboim
Amnon
A
Department of Materials and Interfaces and Chemical Research Support, The Weizmann Institute of Science, Rehovot 76100, Israel.
Daube
Shirley S
SS
Bar-Ziv
Roy
R
D004247:DNA / Q000032:analysis / Q000235:genetics; D005813:Genes, Synthetic / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics
D004247:DNA