Regulation of the activity and expression of ERK8 by DNA damage.
We have investigated the agonists that activate transfected extracellular signal-regulated kinase 8 (ERK8) in cells, and have found that the most potent activators are hydrogen peroxide, DNA alkylating and cross-linking agents and the poly (ADP-ribose) polymerase inhibitor KU-0058948. The feature shared by all these agents is that they lead to the accumulation of single strand breaks in DNA, suggesting a role for ERK8 in the response to, or repair of, DNA single strand breaks. The DNA alkylating agent MMS also induced the disappearance of endogenous ERK8 by a proteasome-dependent mechanism.
Klevernic
Iva V
IV
MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Sir James Black Centre, Dundee, DD1 5EH Scotland, UK.
Martin
Niall M B
NM
Cohen
Philip
P
D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology; D004249:DNA Damage; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005720:Gamma Rays; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000528:radiation effects*; D045325:HCT116 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006918:Hydroxyurea; D007668:Kidney / Q000166:cytology; D016877:Oxidants / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection
D000970:Antineoplastic Agents; D016877:Oxidants; D006861:Hydrogen Peroxide; D048049:Extracellular Signal-Regulated MAP Kinases; C456320:MAPK15 protein, human; D002945:Cisplatin; D006918:Hydroxyurea