title: Hypothalamic proline-rich polypeptide enhances bone marrow colony-forming cell proliferation and stromal progenitor cell differentiation.
abstract: The AGAPEPAEPAQPGVY proline-rich peptide (PRP-1) was isolated from neurosecretory granules of the bovine neurohypophysis; it is produced by N. supraopticus and N. paraventricularis. It has been shown that PRP-1 has many potentially beneficial biological effects, including immunoregulatory, hematopoietic, antimicrobial, and antineurodegenerative properties. Here we showed that PRP increased colony-forming cell (CFC) proliferation in rat bone marrow (BM) cells in vivo. In PRP-treated rat BM, the CFU number at day 7 and day 14 was considerably increased in comparison with untreated rat BM and no difference was found at day 21 and day 28. The related peptide [arg8]vasopressin did not reveal CFC proliferation. PRP failed to farther increase CFC proliferation in vitro in BM obtained from PRP-treated or untreated rats. After 3-4 days of human BM stromal cell cultivation in the presence of 2-20 microg/ml PRP the appearance of cells expressing CD15, CD10, CD11a, CD11b, CD3, CD4, and CD16 surface antigens did not differ from the untreated cells. PRP increased the appearance of CD14-positive cells upon 3-4-day incubation with both adult and fetal BM stromal cells. Our results suggest a previously undescribed role for the hypothalamic peptide within neurosecretory hypothalamus-bone marrow humoral axis, because PRP enhances BM colony-forming cell proliferation and stromal cell differentiation.
authors.affiliation: H. Buniatian Institute of Biochemistry, NAS RA, Yerevan, Republic of Armenia. galoyan@sci.am
authors.forename: A A
authors.initials: AA
authors.lastname: Galoyan
authors.forename: L I
authors.initials: LI
authors.lastname: Korochkin
authors.forename: E J
authors.initials: EJ
authors.lastname: Rybalkina
authors.forename: G V
authors.initials: GV
authors.lastname: Pavlova
authors.forename: I N
authors.initials: IN
authors.lastname: Saburina
authors.forename: E I
authors.initials: EI
authors.lastname: Zaraiski
authors.forename: N A
authors.initials: NA
authors.lastname: Galoyan
authors.forename: T K
authors.initials: TK
authors.lastname: Davtyan
authors.forename: K B
authors.initials: KB
authors.lastname: Bezirganyan
authors.forename: A V
authors.initials: AV
authors.lastname: Revishchin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides; D001127:Arginine Vasopressin / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D015703:Antigens, CD; D023181:Antimicrobial Cationic Peptides; D009479:Neuropeptides; C540268:PRP-1 peptide; D010455:Peptides; D001127:Arginine Vasopressin