Viewing a humorous film decreases IgE production by seminal B cells from patients with atopic eczema.
OBJECTIVE
Sperms induced IgE production by seminal B cells from patients with atopic eczema via interaction of B cells with galectin-3 on sperms. We studied the effect of viewing a humorous film on IgE production by seminal B cells cultured with sperms.


METHODS
Twenty-four male patients with atopic eczema viewed a humorous film (Modern Times, featuring Charlie Chaplin). Just before and immediately after viewing, semen was collected, and seminal B cells and sperms were purified. Seminal B cells were cultured with sperms and IgE production was measured, while expression of galectin-3 on sperms was assessed.


RESULTS
After viewing the humorous film, IgE production by B cells cultured with sperms was significantly decreased. Moreover, expression of galectin-3 on sperms was reduced.


CONCLUSIONS
Viewing a humorous film reduced galectin-3 expression on sperms, which in turn decreased IgE production by seminal B cells cultured with sperms. These results indicate that viewing a humorous film may be helpful for the study and treatment of local IgE production and allergy in the reproductive tract.
Kimata
Hajime
H
Department of Allergy, Moriguchi-Keijinkai Hospital, Moriguchi, Osaka, Japan. kimata-keijinkai@mkc.zap.ne.jp
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018929:Cell Culture Techniques; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D037502:Galectin 3 / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D027641:Laughter Therapy / Q000379:methods*; D008297:Male; D009040:Motion Pictures; D013090:Spermatocytes / Q000378:metabolism*; D055815:Young Adult
D037502:Galectin 3; D007073:Immunoglobulin E