title: Fibrin adhesive and the vaginal vault synthesis on female rabbits abdominal hysterectomies.
abstract: OBJECTIVE
  To investigate the effectiveness of fibrin glue in comparison with polyglycolic acid suture to promote the closure of rabbit's vaginal vault, after abdominal hysterectomy.
  
  
  METHODS
  Twenty female, adults, New Zealand rabbits, were submitted to abdominal hysterectomy and randomly distributed to polyglycolic acid suture (G-PA / n=10) or fibrin glue closure of vaginal vault (G-FG / n=10). Radiograph study allowed identifying vault vaginal suture disrupter or fistulas to urinary bladder or rectum. Videovaginoscopy study allowed identifying the presence of cellulites, abscess formation, tissue granulation or granuloma. Vaginal cuff burst test allowed to identify by the escape of air bubbles and rupture pression record. Histological sections stained with Picrosirius red allowed the measure of fibrous tissue healing.
  
  
  RESULTS
  The videovaginoscopy identified a significant difference (Fisher Test p<0.3142) of granulation tissue in the animals of G-PA (40%) in comparison with the G-FG (20%). The gross inspection showed the same relation in the granulation tissue occurrence (Fisher test p< 0.1749) with G-PA (50%) and G-FB (20%). The visceral adhesion to the vaginal vault wound was statistical significant (Fisher test p< 0.1749) with G-PA (50%) and G-FG (20%). The pressure of rupture (mm Hg) of the burst test was similar (p<0.0421) in the animals of G-PA (61.5+/-19.3) and G-FG (72.5+/-21.9). The collagen matrix of vault wound healing was similar (p< 0.0231) between the G-PA (31.63+/-15) and the G-FG (23.2+/-13.2).
  
  
  CONCLUSIONS
  The vaginal vault closure using the fibrin glue is a safe and reliable procedure after abdominal hysterectomy in female rabbit model.
authors.affiliation: Surgical Technique Division, Department of Surgery, Rio Grande Federal University, Rio Grande, RS, Brazil.
authors.forename: Ari Gonçalves
authors.initials: AG
authors.lastname: Lima
authors.forename: Murched Omar
authors.initials: MO
authors.lastname: Taha
authors.forename: Henri Chaplin
authors.initials: HC
authors.lastname: Rivoire
authors.forename: Anna Tereza Negrini
authors.initials: AT
authors.lastname: Fagundes
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D034861:Abdominal Wall / Q000601:surgery; D000268:Adhesiveness; D000818:Animals; D004353:Drug Evaluation, Preclinical; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006097:Granulation Tissue; D006099:Granuloma / Q000209:etiology; D007044:Hysterectomy / Q000379:methods*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D011183:Postoperative Complications / Q000209:etiology; D011817:Rabbits; D011897:Random Allocation; D013537:Sutures; D014014:Tissue Adhesives / Q000494:pharmacology*; D014621:Vagina / Q000601:surgery*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D011100:Polyglycolic Acid