title: SNP genotyping using the Sequenom MassARRAY iPLEX platform.
abstract: The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
authors.forename: Stacey
authors.initials: S
authors.lastname: Gabriel
authors.forename: Liuda
authors.initials: L
authors.lastname: Ziaugra
authors.forename: Diana
authors.initials: D
authors.lastname: Tabbaa
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D017931:DNA Primers