Effects of sterols on the development and aging of Caenorhabditis elegans.
Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C. elegans to form certain sterols of unknown function. Since sterol metabolism in C. elegans differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in C. elegans, a sterol-feeding experiment was carried out and several critical parameters, such as brood size, growth rate, and life span, were measured. In addition, the change in lipid distribution in C. elegans can be both qualitatively and quantitatively determined by various methods, including staining and chromatographic techniques. Taken together, the effects of sterols on C. elegans are very prominent and can be easily assessed using the techniques described here.
Lee
Eun-Young
EY
Yonsei Proteome Research Center, Biomedical Proteome Research Center, Department of Biochemistry, Yonsei University, Seoul, Korea.
Jeong
Pan-Young
PY
Kim
Sun-Young
SY
Shim
Yhong-Hee
YH
Chitwood
David J
DJ
Paik
Young-Ki
YK
D000375:Aging / Q000187:drug effects*; D000818:Animals; D001391:Azo Compounds / Q000378:metabolism; D001562:Benzimidazoles / Q000378:metabolism; D049628:Body Size / Q000187:drug effects; D017173:Caenorhabditis elegans / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D002462:Cell Membrane; D002467:Cell Nucleus / Q000378:metabolism; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004032:Diet; D020964:Embryo Loss; D005227:Fatty Acids / Q000032:analysis; D005372:Filipin / Q000378:metabolism; D008017:Life Expectancy; D009281:Naphthalenes; D013194:Staining and Labeling; D013261:Sterols / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000494:pharmacology*; D001371:trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride / Q000494:pharmacology
D001391:Azo Compounds; D001562:Benzimidazoles; D005227:Fatty Acids; D009281:Naphthalenes; D013261:Sterols; D001371:trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride; D005372:Filipin; C016118:Sudan Black B; C017807:bisbenzimide ethoxide trihydrochloride