title: A novel nicotinic acetylcholine receptor subtype in basal forebrain cholinergic neurons with high sensitivity to amyloid peptides.
abstract: Nicotinic acetylcholine receptors (nAChRs) containing alpha7 subunits are thought to assemble as homomers. alpha7-nAChR function has been implicated in learning and memory, and alterations of alpha7-nAChR have been found in patients with Alzheimer's disease (AD). Here we report findings consistent with a novel, naturally occurring nAChR subtype in rodent, basal forebrain cholinergic neurons. In these cells, alpha7 subunits are coexpressed, colocalize, and coassemble with beta2 subunit(s). Compared with homomeric alpha7-nAChRs from ventral tegmental area neurons, functional, presumably heteromeric alpha7beta2-nAChRs on cholinergic neurons freshly dissociated from medial septum/diagonal band (MS/DB) exhibit relatively slow kinetics of whole-cell current responses to nicotinic agonists and are more sensitive to the beta2 subunit-containing nAChR-selective antagonist, dihydro-beta-erythroidine (DHbetaE). Interestingly, presumed, heteromeric alpha7beta2-nAChRs are highly sensitive to functional inhibition by pathologically relevant concentrations of oligomeric, but not monomeric or fibrillar, forms of amyloid beta(1-42) (Abeta(1-42)). Slow whole-cell current kinetics, sensitivity to DHbetaE, and specific antagonism by oligomeric Abeta(1-42) also are characteristics of heteromeric alpha7beta2-nAChRs, but not of homomeric alpha7-nAChRs, heterologously expressed in Xenopus oocytes. Moreover, choline-induced currents have faster kinetics and less sensitivity to Abeta when elicited from MS/DB neurons derived from nAChR beta2 subunit knock-out mice rather than from wild-type mice. The presence of novel, functional, heteromeric alpha7beta2-nAChRs on basal forebrain cholinergic neurons and their high sensitivity to blockade by low concentrations of oligomeric Abeta(1-42) suggests possible mechanisms for deficits in cholinergic signaling that could occur early in the etiopathogenesis of AD and might be targeted by disease therapies.
authors.affiliation: Divisions of Neurology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona 85013-4496, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Yao
authors.initials: Y
authors.lastname: Huang
authors.forename: Fenqin
authors.initials: F
authors.lastname: Xue
authors.forename: Alain
authors.initials: A
authors.lastname: Simard
authors.forename: Jamie
authors.initials: J
authors.lastname: DeChon
authors.forename: Guohui
authors.initials: G
authors.lastname: Li
authors.forename: Jianliang
authors.initials: J
authors.lastname: Zhang
authors.forename: Linda
authors.initials: L
authors.lastname: Lucero
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Michael
authors.initials: M
authors.lastname: Sierks
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Chang
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Lukas
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
mesh_terms: D000109:Acetylcholine / Q000378:metabolism* / Q000494:pharmacology; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D018678:Cholinergic Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D047468:Immunoprecipitation / Q000379:methods; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D009865:Oocytes; D018408:Patch-Clamp Techniques / Q000379:methods; D010446:Peptide Fragments / Q000494:pharmacology*; D016548:Prosencephalon / Q000166:cytology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D000109:Acetylcholine; D016229:Amyloid beta-Peptides; D002795:Choline O-Acetyltransferase; D018678:Cholinergic Agents; D010446:Peptide Fragments; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; C579166:Chrna7 protein, mouse; C579165:Chrna7 protein, rat; C075222:amyloid beta-protein (1-42)