title: Antimicrobial activity of endodontic sealers based on calcium hydroxide and MTA.
abstract: The aim of this study was to evaluate the antimicrobial activity of a new root canal sealer containing calcium hydroxide (Acroseal) and the root canal sealer based on MTA (Endo CPM Sealer), in comparison with traditional sealers (Sealapex, Sealer 26 and Intrafill) and white MTA-Angelus, against five different microorganism strains. The materials and their components were evaluated after manipulation, employing the agar diffusion method. A base layer was made using Müller-Hinton agar (MH) and wells were made by removing agar. The materials were placed into the wells immediately after manipulation. The microorganisms used were: Micrococcus luteus (ATCC9341), Staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC27853), Candida albicans (ATCC 10231), and Enterococcus faecalis (ATCC 10541). The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37 degrees C for 24 h. The results showed that Sealapex and its base paste, Sealer 26 and its powder, Endo CPM Sealer and its powder, white MTA and its powder all presented antimicrobial activity against all strains. Intrafill and its liquid presented antimicrobial activity against all strains except P. aeruginosa and Acroseal was effective only against M. luteus and S. aureus.
authors.affiliation: Restorative Dentistry, Araraquara Dental School, São Paulo State University (UNESP), Araraquara, SP, Brazil. tanomaru@uol.com.br
authors.forename: Juliane M G
authors.initials: JM
authors.lastname: Tanomaru
authors.forename: Mário
authors.initials: M
authors.lastname: Tanomaru-Filho
authors.forename: Juliana
authors.initials: J
authors.lastname: Hotta
authors.forename: Evandro
authors.initials: E
authors.lastname: Watanabe
authors.forename: Izabel Y
authors.initials: IY
authors.lastname: Ito
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001729:Bismuth / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D016982:Micrococcus luteus / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D017640:Silicates / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: C477053:Acroseal; D017607:Aluminum Compounds; D000890:Anti-Infective Agents; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D012459:Salicylates; C080620:Sealer 26; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C528808:intrafill; C086631:mineral trioxide aggregate; C055601:Sealapex; D002126:Calcium Hydroxide; D001729:Bismuth