title: Phage-mediated Shiga toxin 2 gene transfer in food and water.
abstract: Shiga toxin (stx) transduction in various food matrices has been evaluated with lysogens of Stx phages. stx transduction events were observed for many phages under appropriate conditions. Transduction did not occur at low pH and low temperatures. A total of 10(3) to 10(4) CFU ml(-1) was the minimal amount of donor and recipient strains necessary to generate transductants.
authors.affiliation: Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
authors.forename: Lejla
authors.initials: L
authors.lastname: Imamovic
authors.forename: Juan
authors.initials: J
authors.lastname: Jofre
authors.forename: Herbert
authors.initials: H
authors.lastname: Schmidt
authors.forename: Ruth
authors.initials: R
authors.lastname: Serra-Moreno
authors.forename: Maite
authors.initials: M
authors.lastname: Muniesa
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D004926:Escherichia coli / Q000821:virology; D005502:Food / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D022641:Shiga Toxin 2 / Q000235:genetics*; D012760:Shigella / Q000821:virology; D013696:Temperature; D014161:Transduction, Genetic; D037521:Virulence Factors / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D022641:Shiga Toxin 2; D037521:Virulence Factors