SpOPT1, a member of the oligopeptide family (OPT) of the fission yeast Schizosaccharomyces pombe, is involved in the transport of glutathione through the outer membrane of the cell.
A protein involved in the transport of glutathione has been identified, cloned and characterized from the fission yeast Schizosaccharomyces pombe. Database searches revealed the Sz. pombe ORF SPAC29B12.10c as a close homologue to several members of the OPT family, including the Saccharomyces cerevisiae high-affinity glutathione transporter Hgt1p. The gene product of SPAC29B12.10c has been identified as a protein, named SpOPT1, localized within the plasma membrane, transporting the tripeptide glutathione. Disruption of SPAC29B12.10c led to strains inable to grow on media containing glutathione as a sole source of sulphur, due to the inability to internalize the tripeptide. Disruptants contained significantly less glutathione than wild-type cells. Furthermore, DeltaSpopt1 strains were non-viable in a glutathione biosynthesis-defective (Deltagsh2) background. However, it was possible to complement the disruption of Spopt1 by overexpressing the intact ORF in the disrupted strain.
Dworeck
Tamara
T
Institute of Biology IV (Microbiology and Genetics), RWTH Aachen University, Worringer Weg, D-52056 Aachen, Germany.
Wolf
Klaus
K
Zimmermann
Martin
M
D001692:Biological Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D009154:Mutation; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D026901:Membrane Transport Proteins; C086860:glutathione transporter; D005978:Glutathione