[Interaction between an insertion/deletion polymorphism in pepsinogen C and Helicobacter pylori infection in the development of gastric cancer].
OBJECTIVE
This study was designed to investigate the interaction between pepsinogen C(PGC) insertion/deletion polymorphism and Helicobacter pylori(Hp) infection, together with its different subtype strains, in the development of gastric cancer (GC).


METHODS
PGC Genotypes were determined by polymerase chain reaction (PCR) assay in 564 subjects with superficial gastritis (NOR), gastric ulcer (GU), atrophic gastritis (AG) and GC, who were frequency-matched as 1:1. Serum Hp-IgG antibodies were determined by an enzyme linked immunoadsorbent assay (ELISA). Hp genetic subtypes in 171 patients with Hp infection were determined by PCR methods.


RESULTS
In GU, AG and GC, the OR of interaction was 8.69 (P = 0.049), 11.16 (P = 0.02), and 10.61 (P = 0.03), respectively; the interaction index of PGC homozygous allele 1 genotype and Hp infection was 5.40, 6.48 or 4.34, respectively; the attributable proportions were 0.721, 0.770 and 0.697, respectively. In AG and GC, no significant interactions were observed between PGC polymorphism and Hp genetic subtypes.


CONCLUSIONS
The findings of this study suggest that PGC insertion/deletion polymorphism and Hp infection seem to present a positive interaction in the development of gastric cancer. While no interactions may be present between PGC polymorphism and Hp genetic subtypes.
Sun
Li-Ping
LP
Cancer Institute, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhang
Ye
Y
Liu
Yun-En
YE
Chen
Wei
W
Yuan
Yuan
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D005260:Female; D005756:Gastritis / Q000235:genetics; D005757:Gastritis, Atrophic / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics*; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D054643:INDEL Mutation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020115:Pepsinogen C / Q000235:genetics*; D011110:Polymorphism, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000382:microbiology; D013276:Stomach Ulcer / Q000235:genetics; D055815:Young Adult
D020115:Pepsinogen C