DNA modification of live cell surface.
We report a novel approach for the attachment of DNA fragments to the surface of live cells. By using fluorescence microscopy and flow cytometry we demonstrated that our synthetic conjugates of fatty acid with oligonucleotides can be incorporated in plasma membrane and then hybridized with complementary sequences at the cell surface. Method permits to control amount of immobilized DNA on the cell surface. All procedures can be completed within minutes and do not alter cell viability. Using this approach we tethered floating myeloid HL-60 cells to adherent A431 epitheliocytes in a sequence specific fashion. Thus, this method allows rapid and simple DNA multicoding of the cell surface and, therefore, opens new opportunities in manipulating with cell-cell interactions.
Borisenko
Grigory G
GG
Research Institute of Physical-Chemical Medicine, Russian Academy of Science, Moscow 119312, Russia. grigoryb@yahoo.com
Zaitseva
Marina A
MA
Chuvilin
Andrey N
AN
Pozmogova
Galina E
GE
D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*
D005227:Fatty Acids; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA