Quantitative peptidomics reveal brain peptide signatures of behavior.
The honey bee genome predicts approximately 100 peptides from 36 prohormones, but the functions of many of these peptides are unknown. We used differential isotope labeling combined with mass spectrometric analysis to quantify approximately 50% of known bee brain peptides in the context of foraging, with 8 showing robust and dynamic regulation. Some showed differences in brain abundance as a function of experience; specifically, nectar and pollen collection led to quick changes in abundance. These changes were related to the act of food collection, not ingestion, because foragers bring food back to the hive for storage rather than eating it themselves. Other peptide differences in brain abundance were seen in bees that either flew to a nectar feeder or a pollen feeder, but did not yet collect any food. These differences likely reflect well-known predispositions of some bees to collect either nectar or pollen, but not both. Tachykinin, PBAN, and sNPF were among the peptides with the strongest changes in association with nectar and pollen foraging. These peptides are known to be involved in regulating food intake in solitary insects, suggesting an evolutionary connection between that behavior and social foraging. These results demonstrate that it is now possible to use quantitative peptidomics to help determine which brain peptides are bioactive and to elucidate their function in the regulation of behavior.
Brockmann
Axel
A
Department of Entomology, Neuroscience Program, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Annangudi
Suresh P
SP
Richmond
Timothy A
TA
Ament
Seth A
SA
Xie
Fang
F
Southey
Bruce R
BR
Rodriguez-Zas
Sandra R
SR
Robinson
Gene E
GE
Sweedler
Jonathan V
JV
D000818:Animals; D001516:Bees; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000378:metabolism*; D005247:Feeding Behavior / Q000502:physiology*; D006728:Hormones / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D009479:Neuropeptides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011058:Pollen; D040901:Proteomics / Q000379:methods*; D015320:Tachykinins / Q000378:metabolism
D006728:Hormones; D009479:Neuropeptides; D010455:Peptides; D015320:Tachykinins; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea