System-wide molecular evidence for phenotypic buffering in Arabidopsis.
We profiled 162 lines of Arabidopsis for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects, suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs.
Fu
Jingyuan
J
Groningen Bioinformatics Centre, University of Groningen, 9751NN Haren, The Netherlands.
Keurentjes
Joost J B
JJ
Bouwmeester
Harro
H
America
Twan
T
Verstappen
Francel W A
FW
Ward
Jane L
JL
Beale
Michael H
MH
de Vos
Ric C H
RC
Dijkstra
Martijn
M
Scheltema
Richard A
RA
Johannes
Frank
F
Koornneef
Maarten
M
Vreugdenhil
Dick
D
Breitling
Rainer
R
Jansen
Ritsert C
RC
D017360:Arabidopsis / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D002874:Chromosome Mapping; D020869:Gene Expression Profiling; D018745:Genome, Plant; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
D015415:Biomarkers