[Determination of loganin and paeonol in Liuwei Dihuang pills by HPLC amalgamated of double UV waves].
OBJECTIVE
To establish a high performance liquid chromatographic (HPLC) amalgamated to double UV waves method for simultaneous determination of loganin and paeonol in Liuwei Dihuang pills.


METHODS
A HPLC method was developed. The separation was carried out on a Agilent Zorbax SB C18 column (5 microm, 4.6 mm x 250 mm). The mobile phase consisted of water (A) and acetonitrile (B) with linear linear gradient elution [0-8 min, (B) from 1% to 12%; 8-21 min, B keep 12%; 21-40 min, (B) from 12% to 90%; 40-50 min, B keep 90% for 10 min]. The detection was Photodiode Array with the detection wavelengths were at 236 nm and 274 nm. The column temperature being 30 degrees C and the flow rate was 1.0 mL min(-1). Extracting the chromatergraph from 274 nm and 236 nm, we amalgamated the two chromatographs by matlab programmed.


RESULTS
The calibration curves of loganin and paeonol were linear in the ranges of 0.0362-1.09 microg (r =0. 9998) and 0.0450-1.35 microg (r =0.9998), respectively. The average recoveries of loganin and paeonol were 97.3% (RSD 1.4 %) and 103.0% (RSD 1.9%), respectively. Three different batches of Liuwei Dihuang pills were determined with this method.


CONCLUSIONS
This is a more convenient, reasonable and credible quality control method for the traditional Chinese medicine.
Zhao
Hong-Zhi
HZ
Tianjin High-standard Quality Testing Laboratory, Tianjin 300084, China.
Meng
Xian-Sheng
XS
Ye
Ting-Xiang
TX
Liu
Zheng-Hui
ZH
Cheng
Yi
Y
Luo
Guo-An
GA
D000098:Acetophenones / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D039823:Iridoids / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000098:Acetophenones; D004365:Drugs, Chinese Herbal; D039823:Iridoids; C476907:liu-wei-di-huang wan; C013638:paeonol; C059516:loganin