[Determination of environmental estrogens in pharmacy wastewater using solid-phase extraction-gas chromatography/mass spectrometry with derivatization].
A method for the determination of estrone (E1), estradiol (E2), ethinylestradiol (EE2) and estriol (E3) in pharmacy wastewater was developed using solid-phase extraction-gas chromatography/mass spectrometry (SPE-GC/MS) with derivatization. The sample was extracted by an SPE column, derivatized by bis (trimethylsilyl) trifluoroacetamide (BSTFA), and analyzed by GC/MS. The detection limits were 1.8-4.7 ng/L, and the relative standard deviations were 2.3%-9.1% (n = 8). The recoveries of above four environmental estrogen compounds were (94.0 +/- 2.9)% to (101 +/- 3.8)%. The method can be applied in the determination of the estrogenic compounds in wastewater samples successfully. The concentrations of EE2 and E1 in wastewater were 396.6 ng/L and 39.9 ng/L, respectively, and the removal rates of EE2 and E1 were 35%-40% after traditional biological treatment. The results demonstrated that the removal efficiency was not satisfactory and the traditional treatment process of wastewater containing estrogen compounds from pharmaceuticals factory should be improved.
Huang
Cheng
C
Department of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China. hc198412@163.com
Jiang
Liying
L
Chen
Jianmeng
J
Chen
Xiao
X
D004345:Drug Industry; D052244:Endocrine Disruptors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004967:Estrogens / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007220:Industrial Waste / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D052616:Solid Phase Extraction / Q000379:methods*
D052244:Endocrine Disruptors; D004967:Estrogens; D007220:Industrial Waste