dMec: a novel Mi-2 chromatin remodelling complex involved in transcriptional repression.
The ATP-dependent chromatin remodeller Mi-2 functions as a transcriptional repressor and contributes to the suppression of cell fates during development in several model organisms. Mi-2 is the ATPase subunit of the conserved Nucleosome Remodeling and Deacetylation (NuRD) complex, and transcriptional repression by Mi-2 is thought to be dependent on its associated histone deacetylase. Here, we have purified a novel dMi-2 complex from Drosophila that is distinct from dNuRD. dMec (dMEP-1 complex) is composed of dMi-2 and dMEP-1. dMec is a nucleosome-stimulated ATPase that is expressed in embryos, larval tissues and adult flies. Surprisingly, dMec is far more abundant than dNuRD and constitutes the major dMi-2-containing complex. Both dNuRD and dMec associate with proneural genes of the achaete-scute complex. However, despite lacking a histone deacetylase subunit, only dMec contributes to the repression of proneural genes. These results reveal an unexpected complexity in the composition and function of Mi-2 complexes.
Kunert
Natascha
N
Institut für Molekularbiologie und Tumorforschung, Universität Marburg, Marburg, Germany.
Wagner
Eugenia
E
Murawska
Magdalena
M
Klinker
Henrike
H
Kremmer
Elisabeth
E
Brehm
Alexander
A
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006655:Histone Deacetylases / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D021122:Protein Subunits / Q000378:metabolism
D001324:Autoantigens; D029721:Drosophila Proteins; C116095:Mi-2 protein, Drosophila; D021122:Protein Subunits; D006655:Histone Deacetylases; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D000251:Adenosine Triphosphatases