title: Role of E-cadherin N-glycosylation profile in a mammary tumor model.
abstract: Modifications in cell surface glycosylation affecting cell adhesion are common characteristics of transformed cells. This study characterizes the N-glycosylation profile of E-cadherin in models of canine mammary gland adenoma and carcinoma evaluating the importance of these glycosylation modifications in the malignant phenotype. Our results show that the pattern of E-cadherin N-glycosylation in mammary carcinoma is characterized by highly branched N-glycans, increase in sialylation and an expression of few high mannose structures. Detailed mass spectrometry analysis demonstrated a new N-glycosylation site containing a potential complex type N-glycan in E-cadherin from a mammary carcinoma cell line. Our study demonstrates the importance of E-cadherin N-glycans in the process of tumor development and in the transformation to the malignant phenotype.
authors.affiliation: Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Rua Dr Roberto Frias s/n, 4200-465 Porto, Portugal.
authors.forename: Salomé S
authors.initials: SS
authors.lastname: Pinho
authors.forename: Hugo
authors.initials: H
authors.lastname: Osório
authors.forename: Mihai
authors.initials: M
authors.lastname: Nita-Lazar
authors.forename: Joana
authors.initials: J
authors.lastname: Gomes
authors.forename: Célia
authors.initials: C
authors.lastname: Lopes
authors.forename: Fátima
authors.initials: F
authors.lastname: Gärtner
authors.forename: Celso A
authors.initials: CA
authors.lastname: Reis
mesh_terms: D000236:Adenoma / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006031:Glycosylation
chemical_list: D015820:Cadherins