Adenoviral transduction of hTGF-beta1 enhances the chondrogenesis of bone marrow derived stromal cells.
TGF-beta1 plays a necessary and important role in the induction of chondrogenic differentiation of bone marrow stromal cells (BMSCs). In this study, porcine BMSCs were infected with a replication-deficient adenovirus expression vector carrying the hTGF-beta1 gene. The transduced BMSCs were cultured as pelleted micromasses in vitro for 21 days, seeded onto disk-shaped PGA scaffolds for 3 days and subsequently implanted into the subcutaneous tissue of mice. BMSCs transduced with AdhTGF-beta1 expressed and secreted more hTGF-beta1 protein in vitro than those of the control group. Histological and immunohistological examination of the pellets revealed robust chondrogenic differentiation. Tissues made from cells transduced with AdhTGF-beta1 exhibited neocartilage formation after 3 weeks in vivo. The neocartilage occupied 42 +/- 5% of the total tissue volume which was significantly greater than that of the control group. Furthermore, there was extensive staining for sulfated proteoglycans and type II collagen in the AdhTGF-beta1 group compared to controls, and quantification of GAG content showed significantly greater amounts of GAG in experimental groups. The results demonstrate that transfer of hTGF-beta1 into BMSCs via adenoviral transduction can induce chondrogenic differentiation in vitro and enhance chondrogenesis in vivo.
Xia
Wanyao
W
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Tissue Engineering Center, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China.
Jin
Yu-Qing
YQ
Kretlow
James D
JD
Liu
Wei
W
Ding
Wenlong
W
Sun
Hengyun
H
Zhou
Guangdong
G
Zhang
Wenjie
W
Cao
Yilin
Y
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001853:Bone Marrow; D002478:Cells, Cultured; D020219:Chondrogenesis; D020007:Implants, Experimental; D051379:Mice; D017154:Stromal Cells / Q000502:physiology*; D013552:Swine; D014161:Transduction, Genetic; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis* / Q000235:genetics
D053773:Transforming Growth Factor beta1