title: Fast-fluorescence dynamics in nonratiometric calcium indicators.
abstract: The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne, Quantum Architecture Group, Lausanne, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Gersbach
authors.forename: D L
authors.initials: DL
authors.lastname: Boiko
authors.forename: C
authors.initials: C
authors.lastname: Niclass
authors.forename: C C H
authors.initials: CC
authors.lastname: Petersen
authors.forename: E
authors.initials: E
authors.lastname: Charbon