Accurate inference of subtle population structure (and other genetic discontinuities) using principal coordinates.
BACKGROUND
Accurate inference of genetic discontinuities between populations is an essential component of intraspecific biodiversity and evolution studies, as well as associative genetics. The most widely-used methods to infer population structure are model-based, Bayesian MCMC procedures that minimize Hardy-Weinberg and linkage disequilibrium within subpopulations. These methods are useful, but suffer from large computational requirements and a dependence on modeling assumptions that may not be met in real data sets. Here we describe the development of a new approach, PCO-MC, which couples principal coordinate analysis to a clustering procedure for the inference of population structure from multilocus genotype data.


RESULTS
PCO-MC uses data from all principal coordinate axes simultaneously to calculate a multidimensional "density landscape", from which the number of subpopulations, and the membership within subpopulations, is determined using a valley-seeking algorithm. Using extensive simulations, we show that this approach outperforms a Bayesian MCMC procedure when many loci (e.g. 100) are sampled, but that the Bayesian procedure is marginally superior with few loci (e.g. 10). When presented with sufficient data, PCO-MC accurately delineated subpopulations with population F(st) values as low as 0.03 (G'(st)>0.2), whereas the limit of resolution of the Bayesian approach was F(st) = 0.05 (G'(st)>0.35).


CONCLUSIONS
We draw a distinction between population structure inference for describing biodiversity as opposed to Type I error control in associative genetics. We suggest that discrete assignments, like those produced by PCO-MC, are appropriate for circumscribing units of biodiversity whereas expression of population structure as a continuous variable is more useful for case-control correction in structured association studies.
Reeves
Patrick A
PA
United States Department of Agriculture, Agricultural Research Service, National Center for Genetic Resources Preservation, Fort Collins, Colorado, United States of America.
Richards
Christopher M
CM
D000465:Algorithms; D001499:Bayes Theorem; D016000:Cluster Analysis; D003198:Computer Simulation; D005786:Gene Expression Regulation; D051456:Gene Flow; D005787:Gene Frequency; D017343:Genes, Plant; D005828:Genetics, Population; D018745:Genome, Plant; D005838:Genotype; D008957:Models, Genetic; D015233:Models, Statistical; D008962:Models, Theoretical; D015203:Reproducibility of Results