Quantitative identification of teratoma tissues formed by human embryonic stem cells with TeratomEye.
An automated vision system, TeratomEye, was developed for the identification of three representative tissue types: muscle, gut and neural epithelia which are commonly found in teratomas formed from human embryonic stem cells. Muscle tissue, a common structure was identified with an accuracy of 90.3% with high specificity and sensitivity greater than 90%. Gut epithelia were identified with an accuracy of 87.5% with specificity and sensitivity greater than 80%. Neural epithelia which were the most difficult structures to distinguish gave an accuracy of 47.6%. TeratomEye is therefore useful for the automated identification of differentiated tissues in teratoma sections.
Oh
Steve Kah Weng
SK
Stem Cell Group, Bioprocessing Technology Institute, A*STAR, 20 Biopolis Way #06 - 01 Centros, Singapore, 138668, Singapore. steve_oh@bti.a-star.edu.sg
Chua
Pauline
P
Foon
Koh Lin
KL
Ng
Emily
E
Chin
Angela
A
Choo
Andre Boon Hwa
AB
Srinivasan
Rajagopalan
R
D002454:Cell Differentiation; D003584:Cytological Techniques / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D032342:Muscle Cells / Q000166:cytology*; D046569:Neuroepithelial Cells / Q000166:cytology*