title: The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice.
abstract: Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.
authors.affiliation: Department of Pediatric Development and Behavioral Shanghai, Children's Medical Center, School of Medicine, Shanghai Jiao Tung University, Shanghai, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Jin
authors.forename: Cong-Huai
authors.initials: CH
authors.lastname: Yan
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Shen
mesh_terms: D000818:Animals; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000628:therapy*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006632:Histamine / Q000032:analysis / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology* / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010047:Ovalbumin / Q000276:immunology; D010957:Plasmids; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D007070:Immunoglobulin A; D053773:Transforming Growth Factor beta1; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine; D007371:Interferon-gamma; D010047:Ovalbumin; D048271:Chitosan