title: Targeted lipidomics approach for endogenous N-acyl amino acids in rat brain tissue.
abstract: Great effort has been devoted to characterize signaling lipids in central nervous system. This has led to a search for novel strategies to characterize hitherto unknown lipid compositions. Here we developed two methods, one for identification and one for quantification, for N-acyl amino acids, a novel lipid family. The identification method contains a series of purification steps followed by nano-LC/MS/MS and high-throughput screening of the datasets with a potent search algorithm based on fragment ion analysis. MS/MS spectra with good quality can be obtained with 150 fmol of targeted lipids on column with our nano-LC/MS/MS. More than one thousand mass spectra generated using the information dependent acquisition mode of Analyst QS software can be analyzed in 1 min using our home built software. The quantification method utilized the multiple reaction monitoring mode in Analyst software to measure the endogenous levels of N-acyl amino acids in rat brain. Using these two methods we were able to identify and quantify 11 previously reported N-acyl amino acids with endogenous levels ranging from 0.26 to 333 pmol g(-1) wet rat brain.
authors.affiliation: The Gill Center for Biomolecular Science, Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, United States. tanbo76@hotmail.com
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.forename: Y William
authors.initials: YW
authors.lastname: Yu
authors.forename: M Francesca
authors.initials: MF
authors.lastname: Monn
authors.forename: H Velocity
authors.initials: HV
authors.lastname: Hughes
authors.forename: David K
authors.initials: DK
authors.lastname: O'Dell
authors.forename: J Michael
authors.initials: JM
authors.lastname: Walker
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000596:Amino Acids; D008055:Lipids