title: Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2.
abstract: Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.
authors.affiliation: Bioorganische Chemie, Gebäude NW1, Universität Bayreuth, 95440 Bayreuth, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Piontek
authors.forename: Daniel
authors.initials: D
authors.lastname: Varón Silva
authors.forename: Christian
authors.initials: C
authors.lastname: Heinlein
authors.forename: Claudia
authors.initials: C
authors.lastname: Pöhner
authors.forename: Stefano
authors.initials: S
authors.lastname: Mezzato
authors.forename: Petra
authors.initials: P
authors.lastname: Ring
authors.forename: Andreas
authors.initials: A
authors.lastname: Martin
authors.forename: Franz Xaver
authors.initials: FX
authors.lastname: Schmid
authors.forename: Carlo
authors.initials: C
authors.lastname: Unverzagt
mesh_terms: D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D004722:Endoribonucleases / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D004798:Enzymes / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D008026:Ligation; D017510:Protein Folding; D020673:Protein Renaturation
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D006020:Glycopeptides; D006023:Glycoproteins; D004722:Endoribonucleases; C029493:ribonuclease C