Occurrence of the transition of apical architecture and expression patterns of related genes during conversion of apical meristem identity in G2 pea.
G2 pea exhibits an apical senescence delaying phenotype under short-day (SD) conditions; however, the structural basis for its apical development is still largely unknown. In the present study, the apical meristem of SD-grown G2 pea plants underwent a transition from vegetative to indeterminate inflorescence meristem, but the apical meristem of long-day (LD)-grown G2 pea plants would be further converted to determinate floral meristem. Both SD signal and GA(3) treatment enhanced expression of the putative calcium transporter PPF1, and pea homologs of TFL1 (LF and DET), whereas LD signal suppressed their expression at 60 d post-flowering compared with those at 40 d post-flowering. Both PPF1 and LF expressed at the vegetative and reproductive phases in SD-grown apical buds, but floral initiation obviously increased the expression level of PPF1 compared with the unchanged expression level of LF from 40 to 60 d post-flowering. In addition, although the floral initiation significantly enhanced the expression levels of PPF1 and DET, DET was mainly expressed after floral initiation in SD-grown apical buds. Therefore, the main structural difference between LD- and SD-grown apical meristem in G2 pea lies in whether their apical indeterminate inflorescence meristem could be converted to the determinate structure.
Wang
Da-Yong
DY
Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Nanjing 210009, China. dayongw@seu.edu.cn
Li
Qing
Q
Cui
Ke-Ming
KM
Zhu
Yu-Xian
YX
D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005875:Gibberellins / Q000494:pharmacology; D018519:Meristem / Q000235:genetics* / Q000254:growth & development* / Q000528:radiation effects / Q000648:ultrastructure; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects / Q000648:ultrastructure; D010641:Phenotype; D017440:Photoperiod; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
D005875:Gibberellins; D010940:Plant Proteins; C007842:gibberellic acid