Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization.
Nucleocytoplasmic coupling is mediated by outer nuclear membrane (ONM) nesprin proteins and inner nuclear membrane Sun proteins. Interactions spanning the perinuclear space create nesprin-Sun complexes connecting the cytoskeleton to nuclear components. A search for proteins displaying a conserved C-terminal sequence present in nesprins 1-3 identified nesprin 4 (Nesp4), a new member of this family. Nesp4 is a kinesin-1-binding protein that displays Sun-dependent localization to the ONM. Expression of Nesp4 is associated with dramatic changes in cellular organization involving relocation of the centrosome and Golgi apparatus relative to the nucleus. These effects can be accounted for entirely by Nesp4's kinesin-binding function. The implication is that Nesp4 may contribute to microtubule-dependent nuclear positioning.
Roux
Kyle J
KJ
Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610, USA. kroux@ufl.edu
Crisp
Melissa L
ML
Liu
Qian
Q
Kim
Daein
D
Kozlov
Serguei
S
Stewart
Colin L
CL
Burke
Brian
B
D000595:Amino Acid Sequence; D000818:Animals; D018385:Centrosome / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry; D008954:Models, Biological; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
D008565:Membrane Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C485904:SYNE2 protein, human; C539339:nesprin 4 protein, mouse; D049452:Green Fluorescent Proteins; D016547:Kinesins