Normalized Born ratio for fluorescence optical projection tomography.
We present a normalized Born approach for fluorescence optical projection tomography that takes into account tissue absorption properties. This approach can be particularly useful to study fluorochrome distribution within tissue. We use the algorithm to three-dimensionally reconstruct and characterize a fluorescein isothiocyanate containing absorptive phantom and an infarcted mouse heart previously injected with a fluorescent molecular probe.
Vinegoni
Claudio
C
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. cvinegoni@mgh.harvard.edu
Razansky
Daniel
D
Figueiredo
Jose-Luiz
JL
Nahrendorf
Matthias
M
Ntziachristos
Vasilis
V
Weissleder
Ralph
R
D000465:Algorithms; D000818:Animals; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D017202:Myocardial Ischemia / Q000473:pathology*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*