Genome-based expression profiles as a single standardized microarray platform for the diagnosis of experimental interstitial cystitis: an array of 75 genes model.
OBJECTIVE
To investigate the molecular signature underlying experimental interstitial cystitis (IC) using cDNA microarray.


METHODS
Microarray gene expression profiles are studied in bladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis.


RESULTS
Main findings are summarized as follows: firstly, a "75-gene" model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes.


CONCLUSIONS
Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC.
Tseng
Ling-Hong
LH
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and University of Chang Gung School of Medicine, Tao-Yuan, Taiwan. 3g7330@yahoo.com.tw
Chen
Ilene
I
Chen
Ming-Yang
MY
Lee
Chyi-Long
CL
Lo
Tsia-Shu
TS
Lloyd
L Keith
LK
D000818:Animals; D016000:Cluster Analysis; D018856:Cystitis, Interstitial / Q000139:chemically induced / Q000175:diagnosis* / Q000235:genetics*; D036342:Ephrins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D020869:Gene Expression Profiling; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D052250:Nitric Oxide Synthase Type III / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010047:Ovalbumin; D051762:PAX2 Transcription Factor / Q000235:genetics; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism; D013373:Substance P; D059467:Transcriptome; D015854:Up-Regulation; D060449:Wnt Signaling Pathway / Q000235:genetics
D036342:Ephrins; D008070:Lipopolysaccharides; D051762:PAX2 Transcription Factor; C497319:Pax2 protein, mouse; D039961:Semaphorins; D013373:Substance P; D010047:Ovalbumin; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse