Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film.
Based on well-known silver mirror reaction the Ag film was formed on Au film modified by self-assembled monolayer (SAM) of 1,6-hexanedithiol (HDT). The sensitivity of the biosensor based on this Ag/Au film is enhanced compared to that based on Au film. When the surface plasmon resonance (SPR) biosensor based on this Ag/Au film was used to determine human IgG, the range of concentrations of human IgG that could be determined is 0.30-40.00 microg mL(-1). The lowest concentration (0.30 microg mL(-1)) that could be detected was about 8 times lower than that obtained by the biosensor without modification by Ag film (2.50 microg mL(-1)), which demonstrated that the biosensor based on Ag/Au film could make the resonant wavelength move to longer wavelength following with the sensitivity enhancement of the SPR biosensor.
Wang
Liying
L
College of Chemistry, Jilin University, Changchun 130012, PR China.
Sun
Ying
Y
Wang
Jian
J
Zhu
Xiaonan
X
Jia
Fei
F
Cao
Yanbo
Y
Wang
Xinghua
X
Zhang
Hanqi
H
Song
Daqian
D
D015374:Biosensing Techniques / Q000295:instrumentation / Q000592:standards; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012834:Silver; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000592:standards
D007074:Immunoglobulin G; D012834:Silver; D006046:Gold