title: Proteomic analysis of plasma membrane vesicles.
abstract: A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flowcell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived.
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296 Göteborg, Sweden.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Bauer
authors.forename: Max
authors.initials: M
authors.lastname: Davidson
authors.forename: Owe
authors.initials: O
authors.lastname: Orwar
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D002853:Chromatography, Liquid; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D044085:Microfluidics / Q000379:methods; D010455:Peptides / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D010455:Peptides