[Expression analysis of antibacterial peptide genes at different development stages of Musca domestica].
OBJECTIVE
To explore the expression level of antibacterial peptide genes at the different development stages of Musca domestica.


METHODS
Total RNA was extracted from eggs, 1st instar larvae, 2nd instar larvae, 3rd instar larvae, pupae and adults of M. domestica. After the primers for antibacterial peptide (cecropin, defensin and attacin) genes and GAPDH were designed respectively according to the reported M. domestica gene sequences in GenBank, semi-quantitative RT-PCR was performed to detect expression level of these genes in the development stages of M. domestica using GAPDH as inner control.


RESULTS
The antibacterial peptide genes were detected with bands of 210 bp, 300 bp and 650 bp at all development stages of M. domestica. The expression level in the 3rd instar larvae and adults were higher, with a band value of cecropin, defensin and attacin in relation to GAPDH of 1.61, 1.99, 1.62 and 1.47, 1.92, 1.59, respectively; while it was lower at eggs, 1st instar larvae and pupae with a band value of cecropin, defensin and attacin 0.49, 0.49, 0.42 and 0.72, 0.49, 0.64 and 0.65, 0.39, 0.91, respectively.


CONCLUSIONS
The antibacterial peptide genes express at all development stages of M. domestica with an evidently different expression level.
Jin
Xiao-Bao
XB
Department of Parasitology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Wang
Yan
Y
Zhu
Jia-Yong
JY
Ma
Yan
Y
Chu
Fu-Jiang
FJ
Yang
Xiao-Rong
XR
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D017931:DNA Primers; D015870:Gene Expression; D020869:Gene Expression Profiling; D017344:Genes, Insect; D006793:Houseflies / Q000235:genetics* / Q000254:growth & development; D007814:Larva / Q000235:genetics / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction
D023181:Antimicrobial Cationic Peptides; D017931:DNA Primers