Hodgkin lymphoma cell lines are characterized by a specific miRNA expression profile.
Hodgkin lymphoma (HL) is derived from preapoptotic germinal center B cells, although a general loss of B cell phenotype is noted. Using quantitative reverse transcription-polymerase chain reaction and miRNA microarray, we determined the microRNA (miRNA) profile of HL and compared this with the profile of a panel of B-cell non-Hodgkin lymphomas. The two methods showed a strong correlation for the detection of miRNA expression levels. The HL-specific miRNA included miR-17-92 cluster members, miR-16, miR-21, miR-24, and miR-155. Using a large panel of cell lines, we found differential expression between HL and other B-cell lymphoma-derived cell lines for 27 miRNA. A significant down-regulation in HL compared to non-Hodgkin lymphoma was observed only for miR-150. Next, we performed target gene validation of predicted target genes for miR-155, which is highly expressed in HL and is differentially expressed between HL and Burkitt lymphoma. Using luciferase reporter assays, we validated 11 predicted miR-155 target genes in three different HL cell lines. We demonstrated that AGTR1, FGF7, ZNF537, ZIC3, and IKBKE are true miR-155 target genes in HL.
Gibcus
Johan H
JH
Department Pathology & Laboratory Medicine, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands.
Tan
Lu Ping
LP
Harms
Geert
G
Schakel
Rikst Nynke
RN
de Jong
Debora
D
Blokzijl
Tjasso
T
Möller
Peter
P
Poppema
Sibrand
S
Kroesen
Bart-Jan
BJ
van den Berg
Anke
A
D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D035683:MicroRNAs