[CXCR4 combined with P504S or P63 in differential diagnosis of benign and malignant prostatic diseases].
OBJECTIVE
To evaluate the application of CXC chemokine receptor-4 (CXCR4) combined with alpha-methylacyl-CoA racemase (P504S) or P63 protein in the differential diagnosis of benign and malignant prostatic diseases.


METHODS
The EnVision immunohistochemical method was used to detect the expressions of CXCR4, P504S and P63 protein in 40 specimens of PCa not treated by any anticancer therapy and 30 specimens of BPH tissues. The correlation was analyzed between CXCR4 expression and the characteristics of PCa metastasis.


RESULTS
Of the 40 cases of PCa, 33 (82.5%) were stained positive for CXCR4, 37 (92.5%) for P504S and 2 (5%) for P63 protein. Of the 30 cases of BPH, 5 (16.6%) exhibited positivity for CXCR4, 1 for P504S and all for P63. P504S + P63 showed a higher rate of correct diagnosis of PCa than either CXCR4 + P63 or P504S + CXCR4. There was a statistically significant correlation between CXCR4 expression and cancer metastasis (P < 0.05).


CONCLUSIONS
P504S, CXCR4 and P63 are useful tumor markers for the diagnosis and differentiation of benign and malignant prostatic diseases. CXCR4 gives a high rate of correct diagnosis when combined with P504S or P63, and has an important application value in the differential diagnosis of benign and malignant prostatic diseases.
Qu
Feng
F
Department of Urology, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China.
Xing
Yi-Fei
YF
Xiao
Ya-Jun
YJ
Xiao
Chuan-Guo
CG
Guo
Hong-Qian
HQ
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D003937:Diagnosis, Differential; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D019998:Racemases and Epimerases / Q000096:biosynthesis*; D019718:Receptors, CXCR4 / Q000096:biosynthesis*
D014408:Biomarkers, Tumor; C081876:CKAP4 protein, human; C527276:CXCR4 protein, human; D008565:Membrane Proteins; D019718:Receptors, CXCR4; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase