title: Bond strength of a pit-and-fissure sealant associated to etch-and-rinse and self-etching adhesive systems to saliva-contaminated enamel: individual vs. simultaneous light curing.
abstract: This study evaluated in vitro the shear bond strength (SBS) of a resin-based pit-and-fissure sealant [Fluroshield (F), Dentsply/Caulk] associated with either an etch-and-rinse [Adper Single Bond 2 (SB), 3M/ESPE] or a self-etching adhesive system [Clearfil S3 Bond (S3), Kuraray Co., Ltd.] to saliva-contaminated enamel, comparing two curing protocols: individual light curing of the adhesive system and the sealant or simultaneous curing of both materials. Mesial and distal enamel surfaces from 45 sound third molars were randomly assigned to 6 groups (n=15), according to the bonding technique: I - F was applied to 37% phosphoric acid etched enamel. The other groups were contaminated with fresh human saliva (0.01 mL; 10 s) after acid etching: II - SB and F were light cured separately; III - SB and F were light cured together; IV - S3 and F were light cured separately; V - S3 and F were light cured simultaneously; VI - F was applied to saliva-contaminated, acid-etched enamel without an intermediate bonding agent layer. SBS was tested to failure in a universal testing machine at 0.5 mm/min. Data were analyzed by one-way ANOVA and Fisher's test (alpha=0.05).The debonded specimens were examined with a stereomicroscope to assess the failure modes. Three representative specimens from each group were observed under scanning electron microscopy for a qualitative analysis. Mean SBS in MPa were: I-12.28 (+/-4.29); II-8.57 (+/-3.19); III-7.97 (+/-2.16); IV-12.56 (+/-3.11); V-11.45 (+/-3.77); and VI-7.47 (+/-1.99). In conclusion, individual or simultaneous curing of the intermediate bonding agent layer and the resin sealant did not seem to affect bond strength to saliva-contaminated enamel. S3/F presented significantly higher SBS than the that of the groups treated with SB etch-and-rinse adhesive system and similar SBS to that of the control group, in which the sealant was applied under ideal dry, noncontaminated conditions.
authors.affiliation: Department of Pediatric Clinic, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Jaciara Miranda
authors.initials: JM
authors.lastname: Gomes-Silva
authors.forename: Carolina Paes
authors.initials: CP
authors.lastname: Torres
authors.forename: Marta Maria Martins Giamatei
authors.initials: MM
authors.lastname: Contente
authors.forename: Maria Angélica Hueb de Menezes
authors.initials: MA
authors.lastname: Oliveira
authors.forename: Regina Guenka
authors.initials: RG
authors.lastname: Palma-Dibb
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Borsatto
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate; D003743:Dental Enamel; D045662:Dental Etching / Q000379:methods*; D044622:Dental Pellicle; D019232:Dental Restoration Failure; D003799:Dental Stress Analysis; D017220:Dentin-Bonding Agents; D006801:Humans; D055115:Light-Curing of Dental Adhesives / Q000379:methods*; D008422:Materials Testing; D008963:Molar; D010895:Pit and Fissure Sealants; D011140:Polyurethanes; D019279:Resin Cements; D012463:Saliva; D033081:Shear Strength; D013997:Time Factors
chemical_list: D017220:Dentin-Bonding Agents; D010895:Pit and Fissure Sealants; D011140:Polyurethanes; D019279:Resin Cements; C086661:Fluoroshield; D017438:Bisphenol A-Glycidyl Methacrylate