A set of piezoelectric biosensors using cholinesterases.
Piezoelectric sensors have become a versatile tool in biosensorics to study protein-protein and protein-small molecule interactions. Here we present theoretical background on piezoelectric sensors and instructions, how to modify their surface with various recognition elements for cholinesterases. These recognition elements comprise an organophosphate (paraoxon), a cocaine derivative (BZE-DADOO), and a tricyclic, aromatic compound (propidium). Additionally, a guide to the kinetic evaluation of the obtained binding curves is given in this chapter.
Teller
Carsten
C
Department of Analytical Biochemistry, University of Potsdam, Potsdam, Germany.
Halámek
Jan
J
Makower
Alexander
A
Scheller
Frieder W
FW
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002802:Cholinesterases / Q000032:analysis* / Q000737:chemistry*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002802:Cholinesterases