gfap and nestin reporter lines reveal characteristics of neural progenitors in the adult zebrafish brain.
Adult neurogenesis arises from niches that harbor neural stem cells (NSC). Although holding great promise for regenerative medicine, the identity of NSC remains elusive. In mammals, a key attribute of NSC is the expression of the filamentous proteins glial fibrillary acidic protein (GFAP) and NESTIN. To assess whether these two markers are relevant in the fish model, two transgenic zebrafish lines for gfap and nestin were generated. Analysis of adult brains showed that the fusion GFAP-green fluorescent protein closely mimics endogenous GFAP, while the nestin transgene recapitulates nestin at the ventricular zones. Cells expressing the two reporters display radial glial morphology, colocalize with the NSC marker Sox2, undergo proliferation, and are capable of self-renewal within the matrix of distinct thickness in the telencephalon. Together, these two transgenic lines reveal a conserved feature of putative NSC in the adult zebrafish brain and provide a means for the identification and manipulation of these cells in vivo.
Lam
Chen Sok
CS
Institute of Toxicology and Genetics, Forschungszentrum, Karlsruhe Institute of Technology, Karlsruhe, Germany.
März
Martin
M
Strähle
Uwe
U
D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D017930:Genes, Reporter; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D064231:Nestin; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D055748:SOXB1 Transcription Factors