title: [Effect of glutathione and sodium selenite on the metabolism of arsenic in mice exposed to arsenic through drinking water].
abstract: OBJECTIVE
  To explore the effect of glutathione (GSH) and sodium selenite on the metabolism of arsenic in the liver, kidney and blood of mice exposed to iAsIII through drinking water.
  
  
  METHODS
  The mice were randomly divided into control, arsenic, GSH and sodium selenite group, respectively. And each group had eight mice and the mice were exposed to 50 mg/L arsenite by drinking water for 4 weeks. Mice were intraperitoneally injected with GSH (600 mg/kg) and sodium selenite (1 mg/kg) for seven days from the beginning of the fourth week. At the end of the fourth week, liver, kidney and blood were sampled to assess the concentrations of inorganic arsenic (iAs), monomethylarsenic acid (MMA), dimethylarsenic acid (DMA) by hydride generation trapping by ultra-hypothermia coupled with atomic absorption spectrometry.
  
  
  RESULTS
  The liver DMA (233.76 +/- 60.63 ng/g) concentration in GSH group was significantly higher than the arsenic group (218.36 +/- 42.71 ng/g). The concentration of DMA (88.52 +/- 30.86 ng/g) and total arsenic (TAs) (162.32 +/- 49.45 ng/g) in blood of GSH group was significantly higher than those [(45.32 +/- 12.19 ng/g), (108.51 +/- 18.00 ng/g), respectively] of arsenic groups(q values were 3.06, 6.40, 10.72 respectively, P < 0.05). The primary methylated index (PMI) (0.65 +/- 0.050) and secondary methylated index (SMI) (0.55 +/- 0.050) in liver sample of GSH group were significantly higher than those (0.58 +/- 0.056, 0.44 +/- 0. 093) in arsenic group. In blood samples, the PMI (0.85 +/- 0.066) in GSH group was significantly higher than that (0.54 +/- 0.113) in arsenic group (q values were 3.75, 5.26, 4.21 respectively, P < 0.05). However, no significant difference was identified between sodium selenite and arsenic groups in liver, kidney or blood samples. And no significant difference was detected in kidney samples among all arsenic exposing groups.
  
  
  CONCLUSIONS
  Exogenous GSH could promote the methylated metabolism of iAsIII, but sodium selenite showed no significant effects.
authors.affiliation: Department of Environmental and Occupational Health, School of Public Health, China Medical University, Shenyang 110001, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Yu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhong
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Niu
authors.forename: Chun-Qing
authors.initials: CQ
authors.lastname: Qu
authors.forename: Ge-Xin
authors.initials: GX
authors.lastname: Li
authors.forename: Xiu-Qiang
authors.initials: XQ
authors.lastname: Lü
authors.forename: Gui-Fan
authors.initials: GF
authors.lastname: Sun
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Jin
mesh_terms: D000818:Animals; D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D020261:Arsenic Poisoning / Q000378:metabolism*; D004781:Environmental Exposure; D005260:Female; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018038:Sodium Selenite / Q000494:pharmacology*; D014881:Water Supply
chemical_list: D005978:Glutathione; D018038:Sodium Selenite; D001151:Arsenic