title: Unusual sterolic mixture, and 24-isopropylcholesterol, from the sponge Ciocalypta sp. reduce cholesterol uptake and basolateral secretion in Caco-2 cells.
abstract: An unusual sterolic mixture (82.3% of 24-isopropylated sterols) and its major component, 24-isopropylcholesterol, isolated from a marine sponge, Ciocalypta sp. (Halichondriidae), reduce cholesterol uptake, basolateral secretion and ACAT-2 mRNA expression and increase the expression of ABCA1 mRNA in Caco-2 cells. The decreases of cholesterol uptake and secretion induced by 24-isopropylcholesterol alone were more than that of both the sterolic mixture and beta-sitosterol. These data add a new sterol, 24-isopropylcholesterol, to sterols that may reduce intestinal cholesterol absorption.
authors.affiliation: Faculté de Pharmacie, Laboratoire de Biochimie pharmaceutique, Mer-Molécules-Santé, EA 2160, Université de Nantes, Nantes, France.
authors.forename: Hong Quang
authors.initials: HQ
authors.lastname: Do
authors.forename: Laurianne
authors.initials: L
authors.lastname: Van Landeghem
authors.forename: Gaëtane
authors.initials: G
authors.lastname: Wielgosz-Collin
authors.forename: Martin
authors.initials: M
authors.lastname: Takoudju
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Huvelin
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Kornprobst
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Bard
authors.forename: Gilles
authors.initials: G
authors.lastname: Barnathan
authors.forename: Hassan
authors.initials: H
authors.lastname: Nazih
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002784:Cholesterol / Q000378:metabolism*; D003684:Dehydrocholesterols / Q000494:pharmacology; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects; D011161:Porifera; D012333:RNA, Messenger / Q000032:analysis; D012855:Sitosterols / Q000494:pharmacology; D002785:Sterol O-Acyltransferase / Q000235:genetics; D013261:Sterols / Q000494:pharmacology*; D000097587:Sterol O-Acyltransferase 2
chemical_list: D002784:Cholesterol; D003684:Dehydrocholesterols; D012333:RNA, Messenger; D012855:Sitosterols; D002785:Sterol O-Acyltransferase; D013261:Sterols; D000097587:Sterol O-Acyltransferase 2; C516247:24-isopropenyl-22-dehydrocholesterol; C025473:gamma-sitosterol