title: Neurocysticercosis immunodiagnosis using Taenia solium cysticerci crude soluble extract, excretory secretory and lower molecular mass antigens in serum and urine samples of Indian children.
abstract: Neurocysticercosis (NCC), the most common neurological disorder of parasite etiology, results from lodgement of Taenia solium cysticerci in the central nervous system and is now increasingly being recognized in children. The confirmed diagnosis is based collectively on radiological findings and serodiagnostic techniques. The serodiagnostic techniques have variable sensitivity and specificity depending upon the technique, antigens used, location and number of cysts. Crude soluble extract (CSE), excretory secretory (ES) and lower molecular mass (LMM) (10-30 kDa) antigenic fraction of T. solium cysticerci were evaluated for antibody detection in serum and urine samples by ELISA. Serum and urine samples were collected each from 125 clinically suspected and radiologically proven NCC (111 with single Computed Tomography (CT) lesions and 14 with multiple CT lesions) and 125 control subjects (60 with neurological disorders other than NCC, 40 with other parasitic diseases and 25 apparently healthy subjects). The sensitivity of the ELISA with the use of CSE, ES and LMM antigenic fractions was 38.4%, 63.2% and 30.4% with serum (cut off dilution 400), 46.4%, 44% and 47.2% with neat urine and the specificity was 88%, 76.8% and 85.6% with serum (cut off dilution 400), 66.4%, 65.2% and 58.4% with neat urine samples, respectively. The study suggests that detection of antibody to ES antigen in serum samples may serve useful purpose for the serodiagnosis of human NCC.
authors.affiliation: Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. atluripgi@yahoo.com
authors.forename: Subba Rao V
authors.initials: SR
authors.lastname: Atluri
authors.forename: P
authors.initials: P
authors.lastname: Singhi
authors.forename: N
authors.initials: N
authors.lastname: Khandelwal
authors.forename: N
authors.initials: N
authors.lastname: Malla
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000097:blood*; D000947:Antigens, Helminth; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007194:India; D007223:Infant; D008297:Male; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D044967:Serum / Q000469:parasitology*; D041201:Taenia solium / Q000276:immunology* / Q000302:isolation & purification; D014556:Urine / Q000469:parasitology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth