title: Sensitivity enhancement of SPR biosensor with silver mirror reaction on the Ag/Au film.
abstract: Based on well-known silver mirror reaction the Ag film was formed on Au film modified by self-assembled monolayer (SAM) of 1,6-hexanedithiol (HDT). The sensitivity of the biosensor based on this Ag/Au film is enhanced compared to that based on Au film. When the surface plasmon resonance (SPR) biosensor based on this Ag/Au film was used to determine human IgG, the range of concentrations of human IgG that could be determined is 0.30-40.00 microg mL(-1). The lowest concentration (0.30 microg mL(-1)) that could be detected was about 8 times lower than that obtained by the biosensor without modification by Ag film (2.50 microg mL(-1)), which demonstrated that the biosensor based on Ag/Au film could make the resonant wavelength move to longer wavelength following with the sensitivity enhancement of the SPR biosensor.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, PR China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Zhu
authors.forename: Fei
authors.initials: F
authors.lastname: Jia
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Cao
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wang
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Daqian
authors.initials: D
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000592:standards; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012834:Silver; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000592:standards
chemical_list: D007074:Immunoglobulin G; D012834:Silver; D006046:Gold