title: Affinity capturing for targeting proteins into micro and nanostructures.
abstract: Protein immobilization into micro and nanoscaled patterns opens exciting possibilities in fundamental and applied research. Developing efficient capturing techniques while preserving the structural and functional integrity of the proteins on surfaces is a key challenge for surface scientists. In this paper, current techniques for site-specific protein immobilization into engineered surface architectures are reviewed. Fundamental principles for functional protein immobilization on solid supports are discussed and popular affinity-based recognition pairs and their application for capturing proteins into nano and microstructures are presented.
authors.affiliation: Department of Biophysics, University of Osnabrück, Barbarastr. 11, 49076, Osnabrück, Germany.
authors.forename: Changjiang
authors.initials: C
authors.lastname: You
authors.forename: Maniraj
authors.initials: M
authors.lastname: Bhagawati
authors.forename: Andreas
authors.initials: A
authors.lastname: Brecht
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
mesh_terms: D015374:Biosensing Techniques; D008832:Microchemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D011506:Proteins