title: Adipophilin distribution and colocalization with lipid droplets in skeletal muscle.
abstract: Intramyocellular lipids (IMCL) are stored as discrete lipid droplets which are associated with a number of proteins. The lipid droplet-associated protein adipophilin (the human orthologue of adipose differentiation-related protein) is ubiquitously expressed and is one of the predominant lipid droplet-proteins in skeletal muscle. The aim of this study was to investigate the subcellular distribution of adipophilin in human muscle fibres and to measure the colocalization of adipophilin with IMCL. Muscle biopsies from six lean male cyclists (BMI 23.4 +/- 0.4, aged 31 +/- 2 years, W (max) 346 +/- 8) were stained for myosin heavy chain type 1, IMCL, adipophilin and mitochondria using immunofluorescence and viewed with widefield and confocal fluorescence microscopy. The present study shows that like IMCL, the adipophilin content is ~twofold greater in type I skeletal muscle fibres and is situated in the areas between the mitochondrial network. Colocalization analysis demonstrated that 61 +/- 2% of IMCL contain adipophilin. Although the majority of adipophilin is contained within IMCL, 36 +/- 4% of adipophilin is not associated with IMCL. In conclusion, this study indicates that the IMCL pool is heterogeneous, as the majority but not all IMCL contain adipophilin.
authors.affiliation: Exercise Metabolism Research Group, School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. c.s.shaw@bham.ac.uk
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Shaw
authors.forename: Mark
authors.initials: M
authors.lastname: Sherlock
authors.forename: Paul M
authors.initials: PM
authors.lastname: Stewart
authors.forename: Anton J M
authors.initials: AJ
authors.lastname: Wagenmakers
mesh_terms: D000328:Adult; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008297:Male; D008565:Membrane Proteins; D008928:Mitochondria / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D000070780:Perilipin-2
chemical_list: D008055:Lipids; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D010455:Peptides; D000070780:Perilipin-2