Cationic nucleoside lipids based on a 3-nitropyrrole universal base for siRNA delivery.
Cationic nucleoside lipids based on a 3-nitropyrrole universal base were prepared from D-ribose using a straightforward chemical synthesis. Several studies including DLS, TEM, and ethidium bromide (EthBr) assay demonstrated that these amphiphilic molecules form supramolecular organizations of nanometer size in aqueous solutions and are able to bind nucleic acids. siRNA knockdown experiments were performed with these nucleolipids, and we observed protein knockdown activity similar to the siPORT NeoFX positive control. No significant cytotoxicity was found.
Ceballos
Claire
C
Centre Interdisciplinaire de Nanosciences de Marseille CINaM, UPR-CNRS 3118, Université Aix-Marseille II, Luminy, Case 913, 13288 Marseille Cedex 09, France.
Prata
Carla A H
CA
Giorgio
Suzanne
S
Garzino
Frédéric
F
Payet
Dominique
D
Barthélémy
Philippe
P
Grinstaff
Mark W
MW
Camplo
Michel
M
D000818:Animals; D002460:Cell Line; D055785:Gene Knockdown Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D034741:RNA, Small Interfering / Q000378:metabolism*; D012263:Ribonucleosides / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D014162:Transfection / Q000379:methods*
C462529:1-ribofuranosyl-3-nitropyrrole; D008055:Lipids; D011758:Pyrroles; D034741:RNA, Small Interfering; D012263:Ribonucleosides