title: Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication.
abstract: The mechanism by which the influenza A virus genome is packaged into virions is not fully understood. The coding and noncoding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified. Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions. We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5' end of the coding region is important.
authors.affiliation: Division of Virology, Department of Microbiology and Immunology, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ozawa
authors.forename: Junko
authors.initials: J
authors.lastname: Maeda
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Iwatsuki-Horimoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hideo
authors.initials: H
authors.lastname: Goto
authors.forename: Taisuke
authors.initials: T
authors.lastname: Horimoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D001483:Base Sequence; D017930:Genes, Reporter; D009980:Influenza A virus / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D012367:RNA, Viral