title: Differential upregulation of chemokine receptors on CD56 NK cells and their transmigration to the site of infection in tuberculous pleurisy.
abstract: Chemokines and their receptors orchestrate leukocyte recruitment and confer immunity during Mycobacterium tuberculosis infection. The immunoregulatory and cytotoxic activities of natural killer (NK) cells are essential at the site of infection during tuberculous pleurisy. The frequency, subtypes, and expression of phenotype markers and chemokine receptors on NK cells were assessed by flow cytometry in tuberculous (TB) and nontuberculous (NTB) pleural fluid (PF). Chemotaxis was also shown in response to chemokines. A significant decrease in CD56(dim) with no change in CD56(bright) NK cells was observed, while a significant increase in activation markers and Toll-like receptors (TLRs) was observed on TB-PF CD56(bright) NK cells. Significantly increased expression of chemokine receptors CCR1, CCR2 and CCR7 on CD56(bright) and CCR5 on CD56(dim) NK cells was observed in the TB group. Transmigration of TB-PF NK cells was significantly high in response to IL-8, IP-10, MCP-1 and SLC. Transmigrated TB-NK cells showed a significant increase in CXCR2, CCR2 and CCR7 expression. The study suggests that CD56(bright) NK cells may recognize M. tuberculosis directly using TLRs, HLA-DR and express CD69 as an early activation marker. In addition, CC chemokines induce activation signals in chemokine receptors mediating differential NK cell migration to the site. Thus, NK cells act as first direct sensors and effectors in mycobacterial infection.
authors.affiliation: Department of Immunology, Tuberculosis Research Centre ICMR, Chennai, India.
authors.forename: Supriya
authors.initials: S
authors.lastname: Pokkali
authors.forename: Sulochana D
authors.initials: SD
authors.lastname: Das
authors.forename: Anbalagan
authors.initials: A
authors.lastname: Selvaraj
mesh_terms: D000293:Adolescent; D000328:Adult; D019002:CD56 Antigen / Q000032:analysis; D002465:Cell Movement; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry / Q000276:immunology*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D014396:Tuberculosis, Pleural / Q000276:immunology*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D019002:CD56 Antigen; D019707:Receptors, Chemokine