title: Phenotypic and genotypic characterisation of antimicrobial resistance in faecal bacteria from 30 Giant pandas.
abstract: To study the prevalence of antimicrobial resistance in faecal bacteria from Giant pandas in China, 59 isolates were recovered from faecal pats of 30 Giant pandas. Antimicrobial susceptibility testing of the isolates was performed by the standardised disk diffusion method (Kirby-Bauer). Of the 59 study isolates, 32.20% were resistant to at least one antimicrobial and 16.95% showed multidrug-resistant phenotypes. Thirteen drug resistance genes [aph(3')-IIa, aac(6')-Ib, ant(3'')-Ia, aac(3)-IIa, sul1, sul2, sul3, tetA, tetC, tetM, cat1, floR and cmlA] were analysed using four primer sets by multiplex polymerase chain reaction (PCR). The detection frequency of the aph(3')-IIa gene was the highest (10.17%), followed by cmlA (8.47%). The genes aac(6')-Ib, sul2 and tetA were not detected. PCR products were confirmed by DNA sequence analysis. The results revealed that multidrug resistance was widely present in bacteria isolated from Giant pandas.
authors.affiliation: Bioengineering Research Center for Animal Disease Prevention and Control, School of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: An-Yun
authors.initials: AY
authors.lastname: Zhang
authors.forename: Hong-Ning
authors.initials: HN
authors.lastname: Wang
authors.forename: Guo-Bao
authors.initials: GB
authors.lastname: Tian
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Xia
authors.forename: Jun-Ni
authors.initials: JN
authors.lastname: Tang
authors.forename: Li-Kou
authors.initials: LK
authors.lastname: Zou
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods; D001503:Ursidae / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial