High-throughput in vivo genetic and drug screening using femtosecond laser nano-surgery, and microfluidics.
We demonstrate microfluidic devices and imaging technologies for high-speed immobilization, cellular resolution imaging, and femtosecond laser micro-surgery of awake small-animals (C. elegans) for high-throughput in vivo genetic and drug screens on neural degeneration and regeneration.
Rohde
Christopher
C
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, USA.
Gilleland
Cody
C
Samara
Chrysanthi
C
Zeng
Fei
F
Yanik
Mehmet Fatih
MF
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D017785:Photons; D034622:RNA Interference; D013514:Surgical Procedures, Operative