title: Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid.
abstract: The performance of three analyte-specific reagents (ASRs); Cepheid herpes simplex virus (HSV) Typing Primer Probe set (CD), Eragen MultiCode-Rtx HSV-1/2 kit primer mix (ER), and Roche LightCycler HSV-1/2 Primer/Hybridization Probes (RD), was evaluated for detection and typing of herpes simplex virus (HSV-1 and HSV-2) in cerebrospinal fluid (CSF) specimens. Of 68 CSF specimens, HSV-1 was detected in 8 specimens and HSV-2 was detected in 17 specimens. ER detected all 25 HSV-positive specimens, whereas CD and RD detected 24 and 23 HSV-positive specimens, respectively. The results of HSV typing with the 3 ASRs were in complete agreement. The analytical sensitivity of all ASRs was determined to be about 10(1) copies/reaction. Our results demonstrate that the performances of all 3 ASRs are comparable and reliable for routine clinical testing in detection and typing of HSV DNA in CSF.
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and Clinics, Kansas City, MO 64108, USA.
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Selvaraju
authors.forename: Michelle
authors.initials: M
authors.lastname: Wurst
authors.forename: Rebecca T
authors.initials: RT
authors.lastname: Horvat
authors.forename: Rangaraj
authors.initials: R
authors.lastname: Selvarangan
mesh_terms: D002555:Cerebrospinal Fluid / Q000821:virology*; D018792:Encephalitis, Viral / Q000175:diagnosis*; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015345:Oligonucleotide Probes