Advanced substrate fabrication for cell microarrays.
The fabrication and characterization of chemical patterns using a technique that can be readily integrated with methods currently used for the formation of microarrays is presented. A high density poly(ethylene glycol) coating was deposited on glass slides as a background exhibiting low cell attachment properties. Phenylazide modified polymers were then printed on this background. UV irradiation of these polymer arrays resulted in the cross-linking of the polymer spots and their covalent attachment to the surface. Cell attachment was shown to follow the resultant surface chemistry pattern. Furthermore, the use of a robotic contact printer enabled the facile deposition of DNA microarrays on top of and aligned with the polymer microarrays. A transfected cell microarray was generated in this way, demonstrating not only the ability of this platform to limit cell attachment to specific regions, but the suitability for chip-based functional genomics, in particular, and high density cell assays in general.
Hook
Andrew L
AL
School of Chemistry, Physics and Earth Sciences, Flinders University, Bedford Park, Australia. andrew.hook@flinders.edu.au
Thissen
Helmut
H
Voelcker
Nicolas H
NH
D001386:Azides / Q000737:chemistry; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties; D046888:Tissue Array Analysis / Q000379:methods*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D001386:Azides; D020099:Coated Materials, Biocompatible; D046911:Macromolecular Substances; D011092:Polyethylene Glycols; C014747:phenylazide