title: [Primary ocular adnexal lymphoproliferative lesions: clinicopathologic features and genetic alterations].
abstract: OBJECTIVE
  To investigate clinicopathological and genetic characteristics of primary ocular adnexal lymphoproliferative lesions.
  
  
  METHODS
  Clinical, morphological and immunohistochemical features of 37 archival cases of primary ocular adnexal lymphoproliferative lesions were studied including 5 cases of reactive lymphoid hyperplasia and 32 lymphomas retrospectively. Classification of the lymphomas were made according to the WHO classification of tumors of haematopoietic and lymphoid tissues. All cases were studied by interphase fluorescence in situ hybridization (FISH) using dual color break apart probes of IgH, MALT1, bcl-6, c-Myc, bcl-2, CCND1, bcl-10, and FOXP1 for detection of chromosomal aberrations involving IgH, MALT1, bcl-6, c-Myc, bcl-2, cyclinD1, bcl-10 and FOXP1 genes, respectively. FISH with IgH / bcl-2 dual color dual fusion probe was used for detection of t(14;18)(q32;q21)/IgH-bcl-2. CEP18 spectrum orange probe was used for detection of aneuploidy of the chromosome 18.
  
  
  RESULTS
  Among 32 cases of lymphomas, 28 cases (87.5%) were extranodal marginal zone B-cell lymphomas of mucosa associated lymphoid tissue (MALT lymphoma), 2 cases were follicular lymphoma (FL) and 2 cases diffuse large B cell lymphoma (DLBCL). Among the 28 cases of MALT lymphoma, chromosomal aberrations were found in 60.7% (17/28) by interphase FISH analysis. One case showed positive IgH break-apart signal with unknown partner. 16 cases showed three copies of different genes, of which, three copies of MALT1, bcl-6, and c-Myc were identified in 7 cases (25%), 12 cases (43%), and 2 cases (8%) of MALT lymphomas, respectively. In addition, 5 cases showed two genes including three copies of bcl-6 and MALT1 in 4 cases, and three copies of bcl-6 together with c-Myc in one case. Furthermore, all cases with three copies of MALT1 had trisomy 18. t(14;18)(q32;q21) was detected in both follicular lymphomas. Of the 2 DLBCL cases, one showed three copies of bcl-6 together with trisomy 18 and the other one showed three copies of bcl-6 together with IgH and c-Myc rearrangements. Chromosomal aberration was not found in all 5 cases of reactive lymphoid hyperplasia.
  
  
  CONCLUSIONS
  The most common entity of primary ocular adnexal lymphomas is MALT lymphoma and FISH is helpful for their differential diagnosis and classification. Trisomy 18 and three copies of bcl-6 are common chromosomal aberrations in primary ocular adnexal MALT lymphomas.
authors.affiliation: Department of Pathology, Basic Medical College, Capital Medical University, Beijing 100069, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Dong
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Liu
authors.forename: Ha-Si
authors.initials: HS
authors.lastname: Jin
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Ye
authors.forename: Zi-Fen
authors.initials: ZF
authors.lastname: Gao
authors.forename: Xiao-Ge
authors.initials: XG
authors.lastname: Zhou
authors.forename: Ge-Hong
authors.initials: GH
authors.lastname: Dong
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Gong
mesh_terms: D000782:Aneuploidy; D001402:B-Lymphocytes / Q000473:pathology; D020169:Caspases / Q000235:genetics; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D005123:Eye / Q000473:pathology; D005134:Eye Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D016393:Lymphoma, B-Cell / Q000235:genetics*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000473:pathology; D008297:Male; D009154:Mutation; D014178:Translocation, Genetic; D014314:Trisomy
chemical_list: D007143:Immunoglobulin Heavy Chains; D020169:Caspases