A non-contact method and instrumentation to monitor renal ischemia and reperfusion with optical spectroscopy.
The potential of NADH autofluorescence as an in vivo intrinsic optical signature to monitor tissue metabolism is well recognized and supported by experimental results mainly in animal models. In this work, we propose a non-contact implementation of this method using large area excitation and employing a normalization method to account for non-metabolic signal changes. Proof of principle in vivo experiments were carried out using an autofluorescence imaging experimental system and a rat renal ischemia model. A hand-held fiber-optic probe was utilized to test the ability of the signal normalization method to address operational conditions associated with the translation of this method to a clinical setting. Preliminary pre-clinical in vivo test of the probe system was carried out using the same rat model.
Raman
Rajesh N
RN
University of California, Davis, Dept. of Applied Science, One Shields Ave, Davis, CA 95616, USA. topraman@ucdavis.edu
Pivetti
Christopher D
CD
Matthews
Dennis L
DL
Troppmann
Christoph
C
Demos
Stavros G
SG
D000704:Analysis of Variance; D000818:Animals; D005453:Fluorescence; D007511:Ischemia / Q000150:complications*; D007668:Kidney / Q000098:blood supply* / Q000473:pathology*; D008297:Male; D055100:Optical Fibers; D055620:Optical Phenomena; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*; D034421:Sus scrofa; D013997:Time Factors