Heterogeneity of proteins expressed by Brazilian Sporothrix schenckii isolates.
The profiles of proteins present in the exoantigens of Brazilian Sporothrix schenckii isolates were studied and compared by electrophoresis (SDS-PAGE). Thirteen isolates from five different regions of Brazil (1,000 to 2,000 km apart) and ten from a more limited region (200 to 400 km apart within the state of São Paulo) were cultured in Sabouraud, M199 and minimum (MM) media. Qualitative and quantitative differences in the expression of proteins, which varied according to the medium and the isolate, were observed. Fractions with the same MW but varying in intensity were detected, as well as fractions present in 1 isolate but absent in others. Dendrograms were constructed and isolates grouped based on the fractions obtained, irrespective of the intensity. The results showed that Brazilian S. schenckii isolates express different protein profiles, a feature also present in isolates from a more restricted region. The exoantigens were found to have a maximum of 15 protein fractions, ranging in MW from 19-220 KDaltons depending on the medium used for the cultures. These data show the great heterogeneity of Brazilian S. schenckii protein expression.
Fernandes
Geisa Ferreira
GF
Cellular Biology Sector, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Brazil.
Do Amaral
Cristiane Candida
CC
Sasaki
Alexandre
A
Godoy
Patrício Martinez
PM
De Camargo
Zoilo Pires
ZP
D000818:Animals; D000946:Antigens, Fungal / Q000737:chemistry* / Q000378:metabolism; D001938:Brazil; D002415:Cats; D003470:Culture Media / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D013175:Sporothrix / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013174:Sporotrichosis / Q000382:microbiology
D000946:Antigens, Fungal; D003470:Culture Media; D005656:Fungal Proteins; D006023:Glycoproteins