The cellular composition and morphological organization of the rostral migratory stream in the adult human brain.
The rostral migratory stream (RMS) is the major pathway by which progenitor cells migrate from the subventricular zone (SVZ) to the olfactory bulb (OB) in rodents, rabbits and primates. However, the existence of an RMS within the adult human brain has been elusive. Immunohistochemical studies utilising cell-type specific markers for early progenitor cells (CD133), proliferating cells (PCNA), astrocytes and type B cells (GFAP) and migrating neuroblasts (PSA-NCAM), reveal that the adult human RMS is organized into layers containing glial cells, proliferating cells and neuroblasts. In addition, the RMS is arranged around a remnant of the ventricular cavity that extends from the SVZ to the OB as seen by immunohistological staining analysis and electron microscopy, showing the presence of basal bodies and a typical 9+2 arrangement of tubulin in tufts of cilia from all levels of the RMS. Overall, these findings suggest that a pathway of migratory progenitor cells similar to that seen in other mammals is present within the adult human brain and that this pathway could provide for neurogenesis in the human forebrain. These findings contribute to the scientific understanding of adult neurogenesis and establish the detailed cytoarchitecture of this novel neurogenic niche in the human brain.
Kam
Monica
M
Department of Anatomy with Radiology, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
Curtis
Maurice A
MA
McGlashan
Susan R
SR
Connor
Bronwen
B
Nannmark
Ulf
U
Faull
Richard L M
RL
D000071916:AC133 Antigen; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology*; D002465:Cell Movement / Q000502:physiology*; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology; D002923:Cilia / Q000502:physiology; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006416:Hematoxylin; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007211:Indoles; D008297:Male; D046529:Microscopy, Electron, Transmission; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009434:Neural Pathways / Q000033:anatomy & histology* / Q000166:cytology* / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology; D016612:Paraffin Embedding; D010455:Peptides / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats
D000071916:AC133 Antigen; D015703:Antigens, CD; D004396:Coloring Agents; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D006023:Glycoproteins; D007211:Indoles; D019006:Neural Cell Adhesion Molecules; C000606023:PROM1 protein, human; D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; C000606025:Prom1 protein, rat; C018588:Luxol Fast Blue MBS; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin