[Effect of Lactobacillus rhamnosus GG on immune response: experiment with autoimmune mice induced by Campylobacter jejuni].
OBJECTIVE
To investigate the immune effects of Lactobacillus rhamnosus GG (LGG) on immune response.


METHODS
Fourty ICR mice were randomly divided into 4 groups, normal control (N) group, model (M) group, Lactobacillus (LGG) group, and hydrocortisone (HC) group. The mice were induced by Campylobacter jejuni with complete Freund's adjuvant as autoimmune animal model except N group. From the fifteenth day after primary immunization, each group was given different administration through peritoneum route for 7 days. Twenty-five days after the model establishment, the mice were killed. Then the blood sample was collected the undergo ELISA to detect the level of ds-DNA antibody and interferon (IFN)-gamma. The proliferation activities of the B and T cells were examined. The livers and kidneys were taken out to undergo microscopy.


RESULTS
The ds-DNA antibody levels of the HC and LGG groups were not significant different from that of the M group (both P > 0.05). The proliferation activities of T and B cells and IFN-gamma level of the groups LGG were all significantly lower than those of the group M [0.42 +/- 0.05 vs 0.60 +/- 0.09, 0.43 +/- 0.05 vs 0.60 +/- 0.09, (184 +/- 16) vs (195 +/- 6) pg/ml, all P < 0.01].


CONCLUSIONS
LGG inhibits the immune response induced by CJ to a certain degree.
Zhang
Qiong
Q
Wuhu Center for Disease Control and Prevention, Wuhu 241000, China. zhangqiong36@sohu.com
Chen
Qi-yu
QY
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002169:Campylobacter Infections / Q000276:immunology*; D016123:Campylobacter jejuni / Q000276:immunology; D049109:Cell Proliferation; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007778:Lactobacillus / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008813:Mice, Inbred ICR; D013601:T-Lymphocytes / Q000276:immunology
D007371:Interferon-gamma