Determination of zinc using carbonic anhydrase-based fluorescence biosensors.
This chapter summarizes the use of carbonic anhydrase (CA)-based fluorescent indicators to determine free zinc in solution, in cells, and in subcellular organelles. Expression (both in situ and in vitro) and preparation of CA-based indicators are described, together with techniques of their use, and procedures to minimize contamination. Recipes for zinc buffers are supplied.
Bozym
Rebecca
R
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Hurst
Tamiika K
TK
Westerberg
Nissa
N
Stoddard
Andrea
A
Fierke
Carol A
CA
Frederickson
Christopher J
CJ
Thompson
Richard B
RB
D015374:Biosensing Techniques; D002256:Carbonic Anhydrases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D015032:Zinc / Q000032:analysis*
D002256:Carbonic Anhydrases; D015032:Zinc