Determination of the phenoxyacid herbicides MCPA, mecoprop and 2,4-D in kidney tissue using liquid chromatography with electrospray tandem mass spectrometry.
An analytical method was developed to determine the phenoxyacid herbicides 2,4-D, MCPA and mecoprop in kidney tissue from animals where poisoning is suspected. Samples were Soxhlet extracted using diethyl ether and the extracts cleaned-up using anion exchange solid phase extraction cartridges. Analysis was performed using liquid chromatography with negative-ion electrospray tandem mass spectrometry (LC-MS/MS). The method was evaluated by analysing control kidney samples fortified at 1 and 5 mg/kg. Mean recoveries ranged from 82 to 93% with relative standard deviations from 3.2 to 19%. The limit of detection was estimated to be 0.02 mg/kg.
Charlton
Andrew J A
AJ
Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK. andrew.charlton@csl.gov.uk
Stuckey
Vicki
V
Sykes
Mark D
MD
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000302:isolation & purification / Q000506:poisoning; D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification / Q000506:poisoning; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D007668:Kidney / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry
D015084:2,4-Dichlorophenoxyacetic Acid; C008340:mecoprop; D008456:2-Methyl-4-chlorophenoxyacetic Acid