The generation and properties of human macrophage populations from hemopoietic stem cells.
Information about the development and function of human macrophage lineage populations, such as osteoclasts, is limited because of the lack of defined in vitro systems for their large-scale generation. Two M-CSF-containing cytokine cocktails were found under serum-free conditions to expand dramatically and to differentiate over time human CD34(+) hemopoietic stem cells into nonadherent and adherent macrophage populations. These populations exhibited increasing degrees of maturity over a 3-week period characterized by morphology, surface marker expression (CD11b, CD86, CD64, CD14, and c-Fms), phagocytic function, and gene-expression profiling using quantitative PCR and microarray analysis (principal component analysis, k-means clustering, and gene ontology classification). As assessed by the last criterion, the adherent population obtained at 3 weeks from the one protocol tested had high similarity to the well-studied peripheral blood monocyte-derived macrophages. The one population tested could be induced to differentiate into osteoclasts in the presence of M-CSF and receptor activator of NF-kappaB ligand, as judged by morphology, gene expression, and bone-resorbing ability. In addition to the large numbers of macrophage lineage cells able to be produced, this replicating system may be suitable for the molecular analysis of macrophage lineage commitment and progression and for gene targeting and delivery.
Way
Kerrie J
KJ
Department of Medicine and CRC for Chronic Inflammatory Diseases, The University of Melbourne, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Dinh
Hang
H
Keene
Martin R
MR
White
Kirby E
KE
Clanchy
Felix I L
FI
Lusby
Patricia
P
Roiniotis
John
J
Cook
Andrew D
AD
Cassady
A Ian
AI
Curtis
David J
DJ
Hamilton
John A
JA
D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D016000:Cluster Analysis; D016895:Culture Media, Serum-Free; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D010587:Phagocytosis
D018952:Antigens, CD34; D015415:Biomarkers; D016895:Culture Media, Serum-Free; D016173:Macrophage Colony-Stimulating Factor