Characterization of cellulose production in Escherichia coli Nissle 1917 and its biological consequences.
Bacterial species of the Enterobacteriaceae family produce cellulose and curli fimbriae as extracellular matrix components, and their synthesis is positively regulated by the transcriptional activator CsgD. In this group of bacteria, cellulose biosynthesis is commonly regulated by CsgD via the GGDEF domain protein AdrA, a diguanylate cyclase that produces cyclic-diguanylic acid (c-di-GMP), an allosteric activator of cellulose synthase. In the probiotic Escherichia coli strain Nissle 1917 and its recent clonal isolates, CsgD activates the production of curli fimbriae at 28 degrees C, but neither CsgD nor AdrA is required for the c-di-GMP-dependent biosynthesis of cellulose at 28 degrees C and 37 degrees C. In these strains, the GGDEF domain protein YedQ, a diguanylate cyclase that activates cellulose biosynthesis in certain E. coli strains, is not required for cellulose biosynthesis and it has in fact evolved into a novel protein. Cellulose production in Nissle 1917 is required for adhesion of bacteria to the gastrointestinal epithelial cell line HT-29, to the mouse epithelium in vivo, and for enhanced cytokine production. The role of cellulose in this strain is in contrast to the role of cellulose in the commensal strain E. coli TOB1. Consequently, the role of cellulose in bacterial-host interaction is dependent on the E. coli strain background.
Monteiro
Cláudia
C
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
Saxena
Inder
I
Wang
Xiaoda
X
Kader
Abdul
A
Bokranz
Werner
W
Simm
Roger
R
Nobles
David
D
Chromek
Milan
M
Brauner
Annelie
A
Brown
R Malcolm
RM
Römling
Ute
U
D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis; D002460:Cell Line; D002482:Cellulose / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019761:Phosphorus-Oxygen Lyases / Q000378:metabolism*; D016415:Sequence Alignment; D015534:Trans-Activators / Q000502:physiology*
D001426:Bacterial Proteins; C499694:CsgD protein, E coli; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C077481:Crl protein, Bacteria; D002482:Cellulose; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase