Chikungunya virus isolated in Lakshadweep islands in the Indian Ocean: evidence of the Central/East African genotype.
Virological investigation was carried out to determine the etiology of suspected Chikungunya fever among humans reported in the Lakshadweep islands in the Indian Ocean. Three out of 23 acute sera samples showed cytopathological changes in Vero cell lines. Further, indirect immunofluorescence antibody test and reverse transcriptase-polymerase chain reaction studies demonstrated the Chikungunya virus etiology during the episode. E1 gene sequence analysis has confirmed the involvement of the Central/East African genotype of the Chikungunya virus.
Paramasivan
Rajaiah
R
Centre for Research in Medical Entomology, Madurai, India. crmeicmr@icmr.org
Philip Samuel
Paulraj
P
Thenmozhi
Velayutham
V
Rajendran
Rathinasamy
R
Victor Jerald Leo
Soosaimanickam
S
Dhananjeyan
Kutty Jagadeeswaran
KJ
Krishnamoorthi
Ranganathan
R
Arunachalam
Natarajan
N
Tyagi
Brij Kishore
BK
D000350:Africa, Central; D000351:Africa, Eastern; D018354:Alphavirus Infections / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D006801:Humans; D019156:Indian Ocean; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis
D000914:Antibodies, Viral