Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis.
The polysaccharide DAEB was isolated and purified from Enteromorpha intestinalis. It consisted of rhamnose, xylose, galactose, and glucose in a molar ratio of 5.36:1.00:0.57:0.64, and had a molecular weight of 46.8 kDa. Mice were treated with three doses of DAEB for 10 consecutive days by oral administration, the tumor inhibition was 61.17%, 67.65% and 70.59% at doses of 100, 200 and 400 mg/kg, respectively. However, no direct cytotoxicity was detected. At dose of 100, 200 and 400 mg/kg, a significant increase (P<0.01) in relative spleen and thymus weight, and production of tumor necrosis factor alpha (TNF-alpha) were observed in DAEB treated groups. We also found that DAEB significantly stimulated lymphocyte proliferation, especially Concanavalin A-induced lymphocyte proliferation in a dose-dependent manner, and augmented phagocytosis and secretion of NO and TNF-alpha in peritoneal macrophages. The results indicated that DAEB had potent anti-tumor activity which may be associated with its potent immunostimulating effect.
Jiao
Lili
L
Laboratory of Biochemistry and Molecular Biology, School of Life Science, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, 130024, China.
Li
Xia
X
Li
Tianbao
T
Jiang
Peng
P
Zhang
Lixia
L
Wu
Mingjiang
M
Zhang
Liping
L
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism; D044445:Ulva / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; C540249:polysaccharide DAEB; D009569:Nitric Oxide