title: Activity of Chitosans in combination with antibiotics in Pseudomonas aeruginosa.
abstract: Chitosan and its derivative water soluble Chitosan oligosaccharide are used in a variety of applications in pharmaceutical preparations. In this study, 2 wild (ATCC 15729 and PAO1) and 2 mutant strains (PT121 and PT149) of P. aeruginosa are investigated for drug-drug interactions in vitro. 10 antimicrobial agents (antibiotics) are combined with different degree of deacetylated Chitosans and Chitosan oligosaccharide. All the chitosans show synergistic activity with sulfamethoxazole, a sulfonamide antimicrobial agent. It is interesting to observe that the MIC value for the MexEF-OprN overexpressing mutant strain of P. aeruginosa is 5 fold higher than the other strains under investigation suggesting a possible role of this efflux pump in Sulfamethoxazole efflux. The findings suggest on the use of chitosans as enhancing agent in combination with antibiotics in pharmaceutical preparations.
authors.affiliation: Biomedical Engineering Research Centre, Nanyang Technological University, Singapore.
authors.forename: San
authors.initials: S
authors.lastname: Tin
authors.forename: Kishore R
authors.initials: KR
authors.lastname: Sakharkar
authors.forename: Chu Sing
authors.initials: CS
authors.lastname: Lim
authors.forename: Meena K
authors.initials: MK
authors.lastname: Sakharkar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013420:Sulfamethoxazole / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D048271:Chitosan; D013420:Sulfamethoxazole