title: Rapid method for multi-residue determination of avermectins in bovine liver using high-performance liquid chromatography with fluorescence detection.
abstract: A simple method using high-performance liquid chromatography and fluorescence detection is presented for the simultaneous determination of the antiparasitic agents avermectins (abamectin, ivermectin and doramectin) in bovine liver. Samples were extracted using acetonitrile and cleaned up on an alumina SPE column and derivatized with trifluoroacetic anhydride. The derivatives were detected using fluorescence detector set at 365 nm excitation and 475 nm emission wavelengths. As the results, the limit of quantification for three avermectins was about 1 microg/kg. Inter-assay showed the mean recoveries of avermectins in bovine liver ranged from 72% to 81%, with RSD less than 9.9%.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, 18 Shuangqing Road, 100085 Haidian District, Beijing, People's Republic of China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Zijian
authors.initials: Z
authors.lastname: Wang
authors.forename: SuYing
authors.initials: S
authors.lastname: Liu
authors.forename: Ziru
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000977:Antiparasitic Agents; D007559:Ivermectin; C019264:avermectin; C084101:doramectin