title: BARREN INFLORESCENCE2 interaction with ZmPIN1a suggests a role in auxin transport during maize inflorescence development.
abstract: Polar auxin transport, mediated by the PIN-FORMED (PIN) class of auxin efflux carriers, controls organ initiation in plants. In maize, BARREN INFLORESCENCE2 (BIF2) encodes a serine/threonine protein kinase co-orthologous to PINOID (PID), which regulates the subcellular localization of AtPIN1 in Arabidopsis. We show that BIF2 phosphorylates ZmPIN1a, a maize homolog of AtPIN1, in vitro and regulates ZmPIN1a subcellular localization in vivo, similar to the role of PID in Arabidopsis. In addition, bif2 mutant inflorescences have lower auxin levels later in development. We propose that BIF2 regulates auxin transport through direct regulation of ZmPIN1a during maize inflorescence development.
authors.affiliation: Department of Biology, The Pennsylvania State University, 208 Mueller Lab, University Park, PA 16802, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Skirpan
authors.forename: Angela Hendrickson
authors.initials: AH
authors.lastname: Culler
authors.forename: Andrea
authors.initials: A
authors.lastname: Gallavotti
authors.forename: David
authors.initials: D
authors.lastname: Jackson
authors.forename: Jerry D
authors.initials: JD
authors.lastname: Cohen
authors.forename: Paula
authors.initials: P
authors.lastname: McSteen
mesh_terms: D000595:Amino Acid Sequence; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D003313:Zea mays / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases