title: The effect of vitamin C on ischemia reperfusion injury because of prolonged tourniquet application with reperfusion intervals.
abstract: We examined the effect of vitamin C on muscle injury distal to the tourniquet which was applied for 4 hours with 10- and 20-minute reperfusion intervals after 2 hours of tourniquet. Sixty-four Sprague-Dawley rats were allocated to 4 randomized groups. After 2 hours tourniquet, 10- and 20-minutes of reperfusion were allowed to half of each group respectively. Afterward an additional 2 hours compression was applied. Except the control group the animals received vitamin C intravenously, before the first tourniquet in Group I, at the reperfusion interval in Group II, and at both times in Group III. Malondialdehyde levels were measured in blood and the tibialis anterior muscle. The muscle was histopathologically examined. The data was evaluated statistically. The effects of timing and the dose of vitamin C on ischemia reperfusion injury remain controversial and there was no statistical difference between 10- and 20-minute reperfusion intervals. But the blood malondialdehyde levels showed that vitamin C has a positive effect on the muscle injury caused by ischemia-reperfusion.
authors.affiliation: Department of Plastic, Reconstructive, and Aesthetic Surgery, Maltepe University, Istanbul, Turkey. burcaktumerdem@yahoo.com
authors.forename: Burcak Tumerdem
authors.initials: BT
authors.lastname: Ulug
authors.forename: Fehime Benli
authors.initials: FB
authors.lastname: Aksungar
authors.forename: Ozgur
authors.initials: O
authors.lastname: Mete
authors.forename: Fatma
authors.initials: F
authors.lastname: Tekeli
authors.forename: Nilgun
authors.initials: N
authors.lastname: Mutlu
authors.forename: Burcu
authors.initials: B
authors.lastname: Calik
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D013997:Time Factors; D014111:Tourniquets
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D001205:Ascorbic Acid