Effects of sesquiterpene lactones on melanogenesis in mouse B16 melanoma cells.
In this study, we examined the effect of sesquiterpene lactones isolated from Calea urticifolia and Tanacetum parthenium (feverfew) on melanogenesis in mouse B16 melanoma cells. In response to 3-isobutyl-1-methylxanthin (IBMX), B16 melanoma cells underwent differentiation characterized by increased melanin biosynthesis. Treatment of sesquiterpene lactones at lower concentration (1 microM) significantly blocked IBMX-induced melanogenesis, but did not induce the inhibitory activity of cell growth. Among them, 2,3-epoxyjuanislamin exhibited a potent inhibitory effect on melanogenesis. Treatment of B16 cells with 2,3-epoxyjuanislamin elicited significant decreases in tyrosinase protein and mRNA levels. These results demonstrated that the inhibitory effects of sesquiterpene lactones on melanin biosynthesis may be due to the suppression of tyrosinase expression.
Ohguchi
Kenji
K
Gifu International Institute of Biotechnology, Kakamigahara, Gifu, Japan. kohguchi@giib.or.jp
Ito
Masaaki
M
Yokoyama
Kouji
K
Iinuma
Munekazu
M
Itoh
Tomohiro
T
Nozawa
Yoshinori
Y
Akao
Yukihiro
Y
D015056:1-Methyl-3-isobutylxanthine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D019659:Asteraceae / Q000737:chemistry; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D007783:Lactones / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D031203:Tanacetum / Q000737:chemistry
D007783:Lactones; D008543:Melanins; D010726:Phosphodiesterase Inhibitors; D010936:Plant Extracts; D012717:Sesquiterpenes; D014442:Monophenol Monooxygenase; D015056:1-Methyl-3-isobutylxanthine