Microbial transformation of bufotalin by Alternaria alternata AS 3.4578.
The microbial transformation of a cytotoxic bufadienolide, bufotalin (1), was carried out. Three transformed products from 1 by Alternaria alternata were isolated. Their structures were characterized as 3-keto-bufotalin (2), 12 beta-hydroxyl-bufotalin (3), and 3-keto-12 beta-hydroxyl-bufotalin (4) based on the extensive NMR studies. Among them, 3 and 4 were new compounds with strong in vitro cytotoxic activities against HeLa cells.
Xin
Xiu-Lan
XL
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Zhan
Li-Bin
LB
Li
Feng-Yun
FY
Ma
Xiao-Chi
XC
Liu
Ke-Xin
KX
Han
Jian
J
Guo
De-An
DA
D000528:Alternaria / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001711:Biotransformation; D002018:Bufanolides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
D000972:Antineoplastic Agents, Phytogenic; D002018:Bufanolides; C004600:bufotalin