Delayed parturition in cloned calves associated with persistently elevated placentomal TGF-beta1 expression.
The objective of this study was to investigate hormonal and TGF-beta(1) characterizations of delayed parturition in the SCNT recipients (Korean native beef cattle: Hanwoo). The SCNT blastocysts produced by Hanwoo fetal fibroblast cells were transferred into the synchronized Hanwoo recipients. The artificially inseminated Hanwoo recipients (AI-R) were used as control. All AI-R were labored by natural delivery. The SCNT recipients (SCNT-R) with no signs of delivery were operated by Caesarean section. The blood and placentomes were collected during parturition. The weight of placentomes in SCNT-R (n=12, 301+/-41.22 g) was significantly higher than that of AI-R (n=10, 204.8+/-24.89 g) (p<0.05). There were significantly lower E2 (p<0.05) or higher P4 (p<0.01) and TGF-beta(1) (p<0.01) levels in the SCNT-R compared to that of AI-R, respectively. The SCNT-R showed a higher placentomal TGF-beta(1) protein level compared to that of AI-R (p<0.01). Interestingly, the TGF-beta(1) protein level in SCNT-R with normal delivery was dramatically decreased as same as AI-R, but it was highly maintained in C-sec at days 250 of pregnancy in AI-R. These results suggest that delayed parturition in clone calving may be associated with persistence of elevated TGF-beta-1 expression in late pregnancy.
Hwang
Seongsoo
S
National Institute of Animal Science, Suwon, Gyeonggi-do 441-706, Republic of Korea.
Chang
Yoo-Min
YM
Ko
Yeoung-Gyu
YG
Yang
Byoung-Chul
BC
Min
Kwan-Sik
KS
Yoon
Jong-Taek
JT
Nho
Whan-Gook
WG
Kim
Chang-Keun
CK
Seong
Hwan-Hoo
HH
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary; D036861:Delivery, Obstetric / Q000662:veterinary; D004958:Estradiol / Q000097:blood; D005260:Female; D005333:Fetus / Q000502:physiology; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary; D009865:Oocytes / Q000166:cytology; D009929:Organ Size; D036801:Parturition / Q000502:physiology*; D010920:Placenta / Q000033:anatomy & histology / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
D053773:Transforming Growth Factor beta1; D011374:Progesterone; D004958:Estradiol