Steady-state bioluminescence of bacterial luciferase using electrochemical regeneration of flavin substrate and its application to inhibitory analysis.
The model system for the biological reaction using a bacterial luciferase (BL) was developed and applied to the inhibitory analysis of the hydrophobic molecules for enzymatic reactions. The homemade flow electrochemical luminescence cell was embedded in the BL reaction system to regenerate the reduced form of the flavin mononucleotide, which is one of the substrates of the BL luminescence reaction, and to measure the luminescence intensity. The constant intensity of the continuous BL luminescence was observed using the continuous-flow BL reaction system. The proposed system was successfully applied to the inhibitory reaction of dodecaneamide on the BL luminescence reaction.
Yamasaki
Shinya
S
Department of Chemistry, Faculty of Sciences, Kyushu University, 4-2-1, Ropponmatsu, Chuo-ku, Fukuoka 810-8560, Japan. yamasaki@mole.rc.kyushu-u.ac.jp
Nakashima
Shinya
S
Yamada
Shuto
S
Takehara
Kô
K
D048908:Aliivibrio fischeri / Q000201:enzymology; D000577:Amides / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D005415:Flavins / Q000737:chemistry* / Q000378:metabolism*; D008156:Luciferases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*; D013379:Substrate Specificity
D000577:Amides; D005415:Flavins; D008156:Luciferases