Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Förster resonance energy transfer.
By tens-of-picosecond resolved fluorescence detection we study Förster resonance energy transfer between a donor and a black-hole-quencher acceptor bound at the 5'- and 3'-positions of a synthetic DNA oligonucleotide. This dual-labelled oligonucleotide is annealed with either the complementary sequence or with sequences differing in one nucleotide at positions near either the ends or the centre of the oligonucleotide. We find donor fluorescence decay times whose values are definitely distinct and discuss the feasibility of single nucleotide polymorphism genotyping by this method.
Andreoni
Alessandra
A
CNISM-CNR and University of Insubria, Department of Physics and Mathematics, Via Valleggio, 11-22100 Como, Italy. alessandra.andreoni@uninsubria.it
Bondani
Maria
M
Nardo
Luca
L
D001483:Base Sequence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005821:Genetic Techniques; D005838:Genotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*