Induction of focal epithelial hyperplasia in tongue of young bk6-E6/E7 HPV16 transgenic mice.
Squamous cell carcinoma (SCC) of the oral cavity is one of the most common neoplasms in the world. During the past 2 decades, the role of high-risk human papilloma virus (HR-HPV) has been studied and the data supporting HPV as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (HNSCC) has accumulated. In order to investigate the role of HR-HPV oncogene expression in early epithelial alterations in vivo, we produced transgenic mice expressing HPV16 early region genes from the promoter of the bovine keratin 6 gene (Tg[bK6-E6/E7]). In this article, we demonstrate that E6/E7 transgene was abundantly expressed and cellular proliferation was increased in the middle tongue epithelia of transgenic mice, and that in the same region young (27 weeks old) Tg[bK6-E6/E7] mice spontaneously developed histological alterations, mainly focal epithelial hyperplasia (FEH).
Ocadiz-Delgado
Rodolfo
R
Department of Genetics & Molecular Biology, CINVESTAV-IPN Mexico, Av. IPN 2508. Colonia San Pedro Zacatenco, CP 07360, Mexico, DF, Mexico.
Marroquin-Chavira
Alberto
A
Hernandez-Mote
Ruth
R
Valencia
Concepción
C
Manjarrez-Zavala
M Eugenia
ME
Covarrubias
Luis
L
Gariglio
Patricio
P
D000818:Animals; D002417:Cattle; D004195:Disease Models, Animal; D017573:Focal Epithelial Hyperplasia / Q000473:pathology / Q000821:virology*; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D053553:Keratin-6 / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D050725:Papillomavirus E7 Proteins; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014062:Tongue Neoplasms / Q000473:pathology / Q000821:virology*
C068651:E6 protein, Human papillomavirus type 16; D053553:Keratin-6; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16