Application of tandem column solid phase extraction and flame atomic absorption spectrometry for the determination of inorganic and organically bound forms of iron in wine.
A tandem column solid phase extraction (SPE) procedure has been devised to examine the fractionation of Fe in wine. Wine was filtered through a 0.45 microm filter and then, the filtrate was driven through an adsorbing Amberlite XAD-7HP column followed by a cation exchange Dowex 50 W-x8-200 column. Three different Fe groupings are discriminated and assessed, including hydrophobic species of Fe bound to phenolic substances and related species (phenolic fraction), cationic species comprising simple Fe ions and labile Fe forms (cationic fraction), in addition to anionic and/or neutral Fe complexes with organic acids (residual fraction). The suitability of the procedure has been evaluated analyzing four bottled red wines. The results obtained were verified using another tandem column assemblage in which an adsorbing Amberlite XAD-16 column was exchanged by the Amberlite XAD-7HP column. The fractionation pattern ascertained for Fe in analyzed wines is discussed in reference to previously published works. In addition, a conditioning treatment and preparation of Amberlite XAD resins have been revised.
Pohl
Pawel
P
Analytical Chemistry Division, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Stanislawa Wyspianskiego 27, 50-370 Wroclaw, Poland.
Prusisz
Bartlomiej
B
D002852:Chromatography, Ion Exchange; D007475:Ion Exchange Resins; D007501:Iron / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014920:Wine / Q000032:analysis*
D007475:Ion Exchange Resins; D007501:Iron