A quantitative peptidomic analysis of peptides related to the endogenous opioid and tachykinin systems in nucleus accumbens of rats following naloxone-precipitated morphine withdrawal.
We have applied a recently developed label-free mass spectrometry based peptidomic approach to identify and quantify a variety of endogenous peptides from rat nucleus accumbens following withdrawal in naloxone-precipitated, morphine-dependent rats of two separate strains. We focused on maturated, partially processed and truncated peptides derived from the peptide precursors proenkephalin, prodynorphin and preprotachykinin. The expression of several identified peptides was dependent on strain and was affected during morphine withdrawal.
Rossbach
Uwe
U
Department of Pharmaceutical Biosciences, Uppsala University, SE-751 24, Uppsala, Sweden.
Nilsson
Anna
A
Fälth
Maria
M
Kultima
Kim
K
Zhou
Qin
Q
Hallberg
Mathias
M
Gordh
Torsten
T
Andren
Per E
PE
Nyberg
Fred
F
D000595:Amino Acid Sequence; D000701:Analgesics, Opioid / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009020:Morphine / Q000378:metabolism*; D009270:Naloxone / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000737:chemistry* / Q000378:metabolism; D018847:Opioid Peptides / Q000032:analysis*; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013375:Substance Withdrawal Syndrome; D015320:Tachykinins / Q000737:chemistry* / Q000378:metabolism
D000701:Analgesics, Opioid; D018847:Opioid Peptides; D011498:Protein Precursors; D020543:Proteome; D015320:Tachykinins; D009270:Naloxone; D009020:Morphine