title: The Hantavirus Glycoprotein G1 Tail Contains Dual CCHC-type Classical Zinc Fingers.
abstract: Hantaviruses are distributed worldwide and can cause a hemorrhagic fever or a cardiopulmonary syndrome in humans. Mature virions consist of RNA genome, nucleocapsid protein, RNA polymerase, and two transmembrane glycoproteins, G1 and G2. The ectodomain of G1 is surface-exposed; however, it has a 142-residue C-terminal cytoplasmic tail that plays important roles in viral assembly and host-pathogen interaction. Here we show by NMR, circular dichroism spectroscopy, and mutagenesis that a highly conserved cysteine/histidine-rich region in the G1 tail of hantaviruses forms two CCHC-type classical zinc fingers. Unlike classical zinc fingers, however, the two G1 zinc fingers are intimately joined together, forming a compact domain with a unique fold. We discuss the implication of the hantaviral G1 zinc fingers in viral assembly and host-pathogen interaction.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045.
authors.forename: D Fernando
authors.initials: DF
authors.lastname: Estrada
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Boudreaux
authors.forename: Dalian
authors.initials: D
authors.lastname: Zhong
authors.forename: Stephen C
authors.initials: SC
authors.lastname: St Jeor
authors.forename: Roberto N
authors.initials: RN
authors.lastname: De Guzman
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D006476:Orthohantavirus / Q000737:chemistry* / Q000502:physiology; D018804:Hantavirus Pulmonary Syndrome / Q000378:metabolism; D006482:Hemorrhagic Fevers, Viral / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000502:physiology; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary / Q000502:physiology; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000378:metabolism; D019065:Virus Assembly / Q000502:physiology; D016335:Zinc Fingers / Q000502:physiology
chemical_list: D014759:Viral Envelope Proteins; C104008:glycoprotein G1, Hantavirus