title: A single residue in the 126-kDa protein of pepper mild mottle virus controls the severity of symptoms on infected green bell pepper plants.
abstract: Infectious cDNA clones originally derived from a mild strain of Pepper mild mottle virus were constructed by replacing residue 649, a critical point for attenuation of this virus, with all possible amino acids. All clones were infectious to pepper plants and induced a variety of symptoms, including no visible symptoms. The results of this study showed that a single amino acid mutation at residue 649 could control the function of the 126- and 183-kDa proteins, replicases with multiple roles in the life cycle of this virus.
authors.affiliation: Research Team for Vectorborne Diseases, National Agricultural Research Center, 3-1-1 Kan-nondai, Tsukuba, Ibaraki, 305-8666, Japan. tuehara@affrc.go.jp
authors.forename: T U
authors.initials: TU
authors.lastname: Ichiki
authors.forename: E N
authors.initials: EN
authors.lastname: Nagaoka
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Sasaya
authors.forename: T
authors.initials: T
authors.lastname: Omura
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D002212:Capsicum / Q000821:virology*; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D010935:Plant Diseases / Q000821:virology*; D017898:Tobamovirus / Q000235:genetics* / Q000472:pathogenicity*; D015678:Viral Structural Proteins / Q000235:genetics / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D015678:Viral Structural Proteins; D037521:Virulence Factors