[Expression of caveolin-1 gene in hepatitis B-related hepatocellular carcinoma and the clinical significance thereof].
OBJECTIVE
To study the correlation between the expression of caveolin-1 in hepatitis B-related hepatocellular carcinoma (HCC) and the relationship thereof with the invasive and metastatic ability of HCC.


METHODS
Real-time PCR and immunohistochemistry were used to detect the expression of caveolin-1 in 123 specimens of HCC tissues obtained during operation. Human HCC cells of the lines MHCC-97L and MHCC-97H were cultured, and Western-blotting was used to detect the caveolin-1 expression in these cells.


RESULTS
The expression level of caveolin-1 in the HCC tissues was significantly lower than that of the adjacent noncancerous tissues (P = 0.026). The caveolin-1 mRNA expression in the HBV-related HCC cells was negatively correlated with the tumor size, expression of AFP, major venous invasion, being single or multiple, pTNM staging, and HBx expression level, factors closed to the prognosis of HCC. The caveolin-1 protein expression of the MHCC-97H cells was significantly lower than that of the MHCC-97L cells.


CONCLUSIONS
Caveolin-1 expression is associated with the invasive and metastatic ability of HCC, low expression of caveolin-1 shows poor prognosis of HCC, and caveolin-1 is probably down-regulated by HBx protein.
Yan
Jun
J
Institute of Hepato-biliary Surgery, of the People's Liberation Army, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Lu
Qian
Q
Cai
Lei
L
Li
Xiao-wu
XW
Dong
Jia-hong
JH
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D051242:Caveolin 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006509:Hepatitis B / Q000150:complications*; D006515:Hepatitis B virus; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C495540:CAV1 protein, human; D051242:Caveolin 1; D012333:RNA, Messenger