Trafficking of storage proteins in developing grain of wheat.
The processing properties of the wheat flour are largely determined by the structures and interactions of the grain storage proteins (also called gluten proteins) which form a continuous visco-elastic network in dough. Wheat gluten proteins are classically divided into two groups, the monomeric gliadins and the polymeric glutenins, with the latter being further classified into low molecular weight (LMW) and high molecular weight (HMW) subunits. The synthesis, folding and deposition of the gluten proteins take place within the endomembrane system of the plant cell. However, determination of the precise routes of trafficking and deposition of individual gluten proteins in developing wheat grain has been limited in the past by the difficulty of developing monospecific antibodies. To overcome this limitation, a single gluten protein (a LMW subunit) was expressed in transgenic wheat with a C-terminal epitope tag, allowing the protein to be located in the cells of the developing grain using highly specific antibodies. This approach was also combined with the use of wider specificity antibodies to compare the trafficking and deposition of different gluten protein groups within the same endosperm cells. These studies are in agreement with previous suggestions that two trafficking pathways occur in wheat, with the proteins either being transported via the Golgi apparatus into the vacuole or accumulating directly within the lumen of the ER. They also suggest that the same individual protein could be trafficked by either pathway, possibly depending on the stage of development, and that segregation of gluten proteins both between and within protein bodies may occur.
Tosi
Paola
P
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. paola.tosi@bbsrc.ac.uk
Parker
Mary
M
Gritsch
Cristina S
CS
Carzaniga
Raffaella
R
Martin
Barry
B
Shewry
Peter R
PR
D005455:Fluorescent Antibody Technique; D005983:Glutens / Q000378:metabolism* / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D012639:Seeds / Q000166:cytology / Q000196:embryology* / Q000378:metabolism* / Q000648:ultrastructure; D014048:Tolonium Chloride; D014908:Triticum / Q000166:cytology / Q000196:embryology* / Q000378:metabolism* / Q000648:ultrastructure
D021122:Protein Subunits; D014048:Tolonium Chloride; D005983:Glutens