Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru.
To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.
Verdonck
Kristien
K
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, Lima 31, Peru.
González
Elsa
E
Maldonado
Fernando
F
Agapito
Doris
D
Van Dooren
Sonia
S
Vandamme
Anne-Mieke
AM
Silva-Santisteban
Alfonso
A
Vanham
Guido
G
Clark
Daniel
D
Gotuzzo
Eduardo
E
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D015490:HTLV-I Infections / Q000175:diagnosis* / Q000453:epidemiology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D015367:Human T-lymphotropic virus 2 / Q000235:genetics*; D006801:Humans; D008297:Male; D010568:Peru / Q000453:epidemiology; D011237:Predictive Value of Tests; D015995:Prevalence