[Establishment of a cisplatin-induced human nasopharyngeal carcinoma drug-resistant cell line and its biological characteristics].
OBJECTIVE
To establish a CDDP-resistant cell line from human nasopharyngeal carcinoma and evaluate its biological characteristics.


METHODS
By continuously exposing and gradually increasing dose of cisplatin (CDDP), a resistant nasopharyngeal carcinoma cell line (HNE1/CDDP) was established. Drug sensitivity of this cell line was detected by MTT assay; the alterations of its biological characteristics were determined using light microscopy, cell counting and flow cytometry (FCM); its ability of adhesion, migration and invasion were also evaluated.


RESULTS
HNE1/CDDP cell line was developed after 10 months with stable resistance to cisplatin with the resistance index was 5.83. HNE1/CDDP cell exhibited cross-resistance to many other chemotherapeutic agents (carboplatin, oxaliplatin and etoposide, etc). The morphology of HNE1/CDDP changed; doubling time prolonged; and the cell number of S-phase and G2/M-phase decreased while of G0/G1 phase increased compared with parental cells. The ability of adhesion, migration and invasion had no difference between the parental and the resistant cells.


CONCLUSIONS
HNE1/CDDP cell line shows the typical and stable resistant phenotype and can be used as a research model.
Lin
Wen
W
Department of Medical Oncology, Affiliated Cancer Hospital, Shantou University Medical College, Shantou, 515031, China.
Li
Derui
D
Lin
Yingcheng
Y
Du
Caiwen
C
Chen
Jiongyu
J
Hong
Chaoqun
C
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D009303:Nasopharyngeal Neoplasms
D000970:Antineoplastic Agents; D002945:Cisplatin