title: Granzyme-b mediated cell death in the spinal cord-injured rat model.
abstract: Spinal cord injury initiates a complex series of inflammatory and immune responses including the influx of monocytes, macrophages, T-cells, NK cells and so on, into the injured area. In the present study, we found a significant increase in the levels of granzyme-b (gra-b) from the first day after the transection until the third day, with decrease in intensity thereafter. The chemokine IP-10/CXCL10 was also found to be elevated along with gra-b correlating with the infiltration of CD-8(+) cytotoxic T lymphocytes (CTLs) into the injured spinal cord. We observed an increase in the levels of the 64 kDa poly ADP ribose polymerase fragment, known to be a signature fragment produced by gra-b. Localization of gra-b in TUNEL positive neurons indicates that gra-b might play a crucial role in neuronal death and contributes to the pathophysiology of spinal cord injury.
authors.affiliation: Department of Biotechnology and Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Andhra Pradesh, India.
authors.forename: Ganta Vijay
authors.initials: GV
authors.lastname: Chaitanya
authors.forename: Mayuri
authors.initials: M
authors.lastname: Kolli
authors.forename: Phanithi Prakash
authors.initials: PP
authors.lastname: Babu
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000378:metabolism; D016923:Cell Death; D002531:Cerebellum / Q000378:metabolism; D054357:Chemokine CXCL10 / Q000378:metabolism; D053804:Granzymes / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000502:physiology; D013997:Time Factors
chemical_list: D016827:CD8 Antigens; D054357:Chemokine CXCL10; C091326:Cxcl10 protein, rat; D011065:Poly(ADP-ribose) Polymerases; D053804:Granzymes; C509215:Gzmb protein, rat; D010751:Phosphopyruvate Hydratase