title: Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
abstract: Schizophrenia is a complex disease, likely to be caused by a combination of serial alterations in a number of genes and environmental factors. The dorsolateral prefrontal cortex (Brodmann's Area 46) is involved in schizophrenia and executes high-level functions such as working memory, differentiation of conflicting thoughts, determination of right and wrong concepts and attitudes, correct social behavior and personality expression. Global proteomic analysis of post-mortem dorsolateral prefrontal cortex samples from schizophrenia patients and non-schizophrenic individuals was performed using stable isotope labeling and shotgun proteomics. The analysis resulted in the identification of 1,261 proteins, 84 of which showed statistically significant differential expression, reinforcing previous data supporting the involvement of the immune system, calcium homeostasis, cytoskeleton assembly, and energy metabolism in schizophrenia. In addition a number of new potential markers were found that may contribute to the understanding of the pathogenesis of this complex disease.
authors.affiliation: Laboratório de Neurociências, Instituto de Psiquiatria, Universidade de São Paulo, Rua. Dr. Ovidio Pires de Campos, no 785, Consolação, São Paulo, SP 05403-010, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Wagner F
authors.initials: WF
authors.lastname: Gattaz
authors.forename: Andrea
authors.initials: A
authors.lastname: Schmitt
authors.forename: Christiane
authors.initials: C
authors.lastname: Rewerts
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Dias-Neto
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002118:Calcium / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006706:Homeostasis; D006801:Humans; D007107:Immune System / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011180:Postmortem Changes; D017397:Prefrontal Cortex / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012559:Schizophrenia / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D009419:Nerve Tissue Proteins; D010455:Peptides; D020543:Proteome; D002118:Calcium