title: FcepsilonRI-mediated thymic stromal lymphopoietin production by interleukin-4-primed human mast cells.
abstract: A significant increase of mRNA expression of thymic stromal lymphopoietin (TSLP) has been reported in the bronchial mast cells (MCs) of asthmatic subjects; however, the mechanism underlying the upregulation of TSLP mRNA and protein remains unknown. FcepsilonRI-mediated activation of human MCs upregulated TSLP mRNA expression by 5.2+/-2.9-fold, while activation of the MCs using lipopolysaccharide and polyriboinosinic:polyribocytidylic acid failed to upregulate TSLP. Stimulation of MCs with interleukin (IL)-4 alone did not affect the TSLP mRNA expression, while pre-incubation of MCs with IL-4 for 48 h significantly enhanced the FcepsilonRI-mediated TSLP mRNA expression (by 53.7+/-15.9-fold; p<0.05) and the amount of TSLP in the cell pellets increased significantly from 23.4+/-4.3 pg mL(-1) to 121.5+/-3.7 pg mL(-1) (p<0.0001). However, the released TSLP was rapidly degraded by proteases that were released by MCs. We identified the population of cells expressing TSLP in the lungs of 16 asthmatic and 11 control subjects by immunohistochemistry. The percentage of TSLP-positive MCs in the total population of MCs was significantly increased in asthmatic airways (p<0.0001). Thus, MCs are able to store TSLP intracellularly and to produce TSLP following aggregation of FcepsilonRI in the presence of IL-4.
authors.affiliation: Division of Molecular Cell Immunology and Allergology, Advanced Medical Research Center, Nihon University Graduate School of Medical Science, Tokyo, Japan. yokayama@med.nihon-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Okayama
authors.forename: S
authors.initials: S
authors.lastname: Okumura
authors.forename: H
authors.initials: H
authors.lastname: Sagara
authors.forename: K
authors.initials: K
authors.lastname: Yuki
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Fueki
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: C
authors.initials: C
authors.lastname: Ra
mesh_terms: D000328:Adult; D001249:Asthma / Q000378:metabolism; D001980:Bronchi / Q000378:metabolism*; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D015847:Interleukin-4 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008407:Mast Cells / Q000166:cytology*; D008875:Middle Aged; D017455:Receptors, IgE / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000378:metabolism*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D017455:Receptors, IgE; D015847:Interleukin-4; D000094632:Thymic Stromal Lymphopoietin