[Analysis of CDR3 sequence of TCR Valpha6, Valpha10 and Vbeta23 oligoclonal T cells in one APL case].
OBJECTIVE
To investigate the clonality and the pattern of CDR3 sequence of TCR Valpha and Vbeta repertoire in patients with acute promyelocytic leukemia (APL).


METHODS
The CDR3 of TCR Valpha 29 and Vbeta 24 subfamily genes were amplified in mononuclear cells from peripheral blood of one case with APL using RT-PCR. The positive PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluate clonality of the detectable TCR Valpha and Vbeta T cells. The products of the oligoclonal T cells were analyzed by sequencing to define the sequence of CDR3.


RESULTS
Oligoclonal T cells expressed TCR Valpha6, Valpha10 or Vbeta23 genes were identified from the APL patient. The molecular characteristics of the CDR3 of Valpha and Vbeta genes rearrangement were Valpha6NJalpha17, Valpha10NJalpha35 and Vbeta23NDbeta1NJbeta2.7. The amino acid sequence of CDR3 region in TCR Valpha 6, Valpha10 and Vbeta23 subfamilies were CAMRENSAGNK, YLCAGDELLWECA or CASSSKWAGGTYEQY, respectively. These sequences were accepted by GenBank (Accession Number EU544946, EU544947 and EU647219).


CONCLUSIONS
Three idiotype Valpha and Vbeta sequences are identified in a case with APL, which is thought that may mediate the host specific immunity response for leukemia cells in APL. It might provide data for farther research on the specific immunotherapy of APL.
Chen
Shao-hua
SH
Institute of Hematology, Medical College of Jinan University, First Affiliated Hospital, Jinan University, Guangzhou, China. jnshaohuachen@163.com
Li
Yang-qiu
YQ
Yang
Li-jian
LJ
Zhou
Yu-bing
YB
Li
Bo
B
Zhang
Xue-li
XL
Luo
Geng-xin
GX
D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA
D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; C528689:Valpha24 protein, human