Technical note: A new and cost-effective method for detection of the bovine acyl-CoA:diacylglycerol acyltransferase 1 K232A polymorphism in cattle.
A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.
Steinberg
R S
RS
Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Pereira
L
L
Lacorte
G A
GA
Peixoto
M G C D
MG
Verneque
R S
RS
Teodoro
R L
RL
Machado
M A
MA
Fonseca
C G
CG
Carvalho
M R S
MR
D000818:Animals; D002417:Cattle / Q000235:genetics*; D003612:Dairying / Q000379:methods*; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*
D051048:Diacylglycerol O-Acyltransferase