title: Simultaneous determination of (fluoro)quinolones antibacterials residues in bovine milk using ultra performance liquid chromatography-tandem mass spectrometry.
abstract: The ultra performance liquid chromatography-tandem mass spectrometry (UPLC) method had been developed for 22 (fluoro)quinolone(QNs) antibacterials in milk with multiple reaction monitoring (MRM) as acquisition mode. The analytes were extracted from the sample using Mcllvaine buffer by ultrasonic bath, and purified by solid-phase extraction (SPE) cartridge. The residue were dried under nitrogen and dissolved in mobile phase before UPLC-MS/MS final analysis. The calibration curve of six concentrations for 22 QNs showed good linearity and the good correlation coefficients (r> or =0.9851) were achieved. The limit range of quantification was 0.008-0.339microg/kg. The recovery range was 63.1-94.6% except flumequine, nalidixic acid and nadifloxacin. The method was precise: the relative standard deviations of the method for milk were not more than 13.12%. The accuracies and sensitivity of the method were good for simultaneous determination of 22 QNs.
authors.affiliation: College of Food Science and Biological Engineering, Zhejiang Gongshang University, Hangzhou 310035, People's Republic of China. hongzh1316@mail.zjgsu.edu.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yiping
authors.initials: Y
authors.lastname: Ren
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Bao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D002417:Cattle; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005260:Female; D024841:Fluoroquinolones / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014465:Ultrasonics
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D024841:Fluoroquinolones