[Expression and significance of telomerase activity of lens epithelial cells in posterior capsule opacification of rabbits].
OBJECTIVE
To explore the expression of telomerase activity of the lens epithelial cells in posterior capsule opacification of rabbits.


METHODS
Clear corneal tunnel phacoemulsification was performed in both eyes of 11 New Zealand rabbits. After the model of posterior capsule opacification succeed in all eyes. Then, telomerase activity of the lens epithelial cells in the equator and posterior capsule opacification of 20 eyes was detected with TRAP-ELISA and TRAP-PAGE techniques.


RESULTS
HepG2 cells were used as positive controls. Telomerase activity was detected in the lens epithelial cells in the equator capsule and posterior capsule opacification in rabbits, which showed several dim gradient stripped electrophoresis pattern. A450 - 690 value of telomerase activity in the equator capsule and posterior capsule opacification was 0.85 +/- 0.23 and 0.67 +/- 0.19, respectively, indicating statistically significant difference (t = 2.526, 0.021; P < 0.05).


CONCLUSIONS
Telomerase activity exists in the lens epithelial cells of posterior capsule opacification in rabbits. The telomerase activity in this area is lower than that in the equator capsule.
Lü
Zhi-gang
ZG
Department of Ophthalmology, Jinhua Central Hospital of Zhejiang Province, Jinhua 321000, China. JhYYLZG@163.com
Huang
Wen-li
WL
Jiang
Yong-xiang
YX
Liu
Tian-jin
TJ
D000818:Animals; D002386:Cataract / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011817:Rabbits; D019098:Telomerase / Q000378:metabolism*
D019098:Telomerase