Application of a new chair-side method for the harvest of mesenchymal stem cells in a patient with nonunion of a fracture of the atrophic mandible--a case report.
OBJECTIVE
This case report describes a new clinical method for chair-side processing of a cell mixture which contains mesenchymal stem cells (MSCs) which was applied for the first time in the treatment of a nonunion of an atrophic fractured mandible.


METHODS
Bone marrow was aspirated and a corticocancellous bone graft was harvested from the iliac crest of a 56-year-old woman with medical comorbidities and a fracture of the atrophic mandible. The fracture was stabilized with a reconstruction bone plate, and mononuclear cells including MSCs were concentrated by centrifugation and applied in combination with a particulate bone transplant. A sample of the grafted cells was characterized by flow cytometric analysis and by their ability to differentiate into various cell types.


RESULTS
The fracture healed uneventfully. No complications occurred during the 4-month follow-up.


CONCLUSIONS
Adding MSCs is a feasible alternative to enhance bone healing. This chair-side method requires little training and no cell laboratory support.
Wongchuensoontorn
Chanchai
C
Department of Oral and Maxillofacial Surgery, Srinakharinwirot University Bangkok, Thailand.
Liebehenschel
Niels
N
Schwarz
Ulrich
U
Schmelzeisen
Rainer
R
Gutwald
Ralf
R
Ellis
Edward
E
Sauerbier
Sebastian
S
D053687:Adult Stem Cells / Q000166:cytology / Q000637:transplantation; D001284:Atrophy / Q000150:complications; D001861:Bone Regeneration / Q000502:physiology; D005260:Female; D017102:Fracture Healing / Q000502:physiology*; D005599:Fractures, Ununited / Q000473:pathology / Q000628:therapy*; D006801:Humans; D008337:Mandibular Fractures / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008875:Middle Aged; D020858:Tissue and Organ Harvesting / Q000379:methods*; D016896:Treatment Outcome