Inhibition of hemolysate-induced iNOS and COX-2 expression by genistein through suppression of NF-small ka, CyrillicB activation in primary astrocytes.
Genistein is a major isoflavone compound from soybean. We investigated the anti-inflammatory properties of genistein in primary astrocytes treated with hemolysate. The results indicated that genistein inhibited the expression of hemolysate induced iNOS and COX-2 mRNA on astrocytes. Furthermore, this compound inhibited the level of hemolysate-stimulated nuclear factor-kappa B (NF-small ka, CyrillicB). Therefore, we suggested that the effect of genistein-mediated inhibition of the expression hemolysate-induced iNOS and COX-2 genes is due to under the suppression of NF-small ka, CyrillicB activation in the transcriptional level.
Lu
Hua
H
Department of Neurosurgery, Jinling Hospital, and The State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 305 East Zhongshan Road, Nanjing 210002, China.
Shi
Ji-Xin
JX
Zhang
Dong-Mei
DM
Wang
Han-Dong
HD
Hang
Chun-Hua
CH
Chen
Hui-Lin
HL
Yin
Hong-Xia
HX
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D012333:RNA, Messenger; D019833:Genistein; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051546:Cyclooxygenase 2