title: Anti-inflammatory phenylpropanoid glycosides from Clerodendron trichotomum leaves.
abstract: The chromatographic separation of MeOH extract from Clerodendron trichotomum Thunberg leaves led to the isolation of three phenylpropanoid compounds. Using spectroscopic methods, the structures of these compounds were determined as beta-(3', 4'-dihydroxyphenyl)ethyl-O-alpha-L-rhamnopyranosyl (1-->3)-beta-D-(4-O-caffeoyl)-glucopyranoside, acteoside (verbascoside) (1), beta-(3', 4'-dihydroxyphenyl)ethyl-O-alpha-L-rhamnopyranosyl (1-->3)-beta-D-(6-O-caffeoyl)-glucopyranoside, isoacteoside (2), beta-(3', 4'-dihydroxyphenyl) ethyl-O-alpha-L-rhamnopyranosyl (1-->3)-beta-D-glucopyranoside, and decaffeoylacteoside (3). We measured the anti-inflammatory activity of these three phenylpropanoid compounds both in vitro (DPPH reduction assay, TBARS assay on Cu (2+)-induced oxidized LDL, PGE(2) assay) and in vivo (acetic acid induced vascular permeability in mice and carrageenan-induced hind paw edema in rats). 80% methanol fraction and acteoside had the activity.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, 156-756, Korea.
authors.forename: Kyoung Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Sungun
authors.initials: S
authors.lastname: Kim
authors.forename: Min Young
authors.initials: MY
authors.lastname: Jung
authors.forename: In Hye
authors.initials: IH
authors.lastname: Ham
authors.forename: Wan Kyunn
authors.initials: WK
authors.lastname: Whang
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D002396:Catechols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D032409:Clerodendrum / Q000737:chemistry; D015232:Dinoprostone / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D009682:Magnetic Resonance Spectroscopy; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D059808:Polyphenols
chemical_list: D019342:Acetic Acid; D000893:Anti-Inflammatory Agents; D001713:Biphenyl Compounds; D002351:Carrageenan; D002396:Catechols; D015232:Dinoprostone; D004187:Disaccharides; D016166:Free Radical Scavengers; D005960:Glucosides; D010636:Phenols; D010851:Picrates; D017392:Thiobarbituric Acid Reactive Substances; C058956:acteoside; D059808:Polyphenols; C064683:isoacteoside; C004931:1,1-diphenyl-2-picrylhydrazyl