title: NF-kappaB decoy oligonucleotides suppress RANTES expression and monocyte chemotactic activity via NF-kappaB inactivation in stromal cells of ectopic endometrium.
abstract: BACKGROUND
  The nuclear factor kappa B (NF-kappaB) pathway is a critical mediator of regulated on activation, normal T cell expressed and secreted (RANTES) gene regulation and therefore represents a potential target for therapy of endometriosis-associated symptoms.
  
  
  OBJECTIVE
  The objective of this study was to investigate the effect of NF-kappaB decoy oligonucleotides (ODNs) on NF-kappaB activation, RANTES expression, and monocyte chemotactic activity in ectopic endometrial stromal cells in vitro.
  
  
  METHODS
  A specific sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantify RANTES expression in ectopic and normal endometrial stromal cells stimulated by interleukin (IL)-1beta. Four hours after transfection of NF-kappaB decoy ODNs, 10 ng/ml IL-1beta was added to induce the ectopic endometrial stromal cells to secrete RANTES. The NF-kappaB activation, RANTES expression, and monocyte chemotactic activity in ectopic endometrial stromal cells were respectively evaluated by electrophoretic mobility shift assay, ELISA, and Boyden chambers.
  
  
  RESULTS
  IL-1beta induced significantly higher levels (P < 0.05) of RANTES expression in a time-dependent manner in ectopic endometrial stromal cells compared with IL-1beta-untreated ectopic and normal endometrial stromal cells. The RANTES accounts for the majority (68%) of the monocyte chemotactic activity in conditioned media of ectopic endometrial stromal cells. In vitro transfection of NF-kappaB decoy ODNs dramatically decreased (P < 0.05) the NF-kappaB activation, RANTES expression, and monocyte chemotactic activity in IL-1beta-induced ectopic endometrial stromal cells.
  
  
  CONCLUSIONS
  NF-kappaB decoy ODNs may exert anti-inflammatory effects in ectopic endometrial stromal cells via the suppression of NF-kappaB activation, RANTES expression, and monocyte chemotactic activity.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 368 North-Jiangdong Road, Nanjing, China. xiuli_2266@163.com
authors.forename: Wang
authors.initials: W
authors.lastname: Xiu-li
authors.forename: Han
authors.initials: H
authors.lastname: Su-ping
authors.forename: Dai
authors.initials: D
authors.lastname: Hui-hua
authors.forename: You
authors.initials: Y
authors.lastname: Zhi-xue
authors.forename: Fu
authors.initials: F
authors.lastname: Shi-long
authors.forename: Lu
authors.initials: L
authors.lastname: Pin-hong
mesh_terms: D002464:Cell Migration Inhibition; D018946:Chemokine CCL5 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002828:Choristoma / Q000378:metabolism / Q000473:pathology; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D009841:Oligonucleotides; D000292:Pelvic Inflammatory Disease / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D011247:Pregnancy; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D052416:Targeted Gene Repair; D014162:Transfection
chemical_list: D018946:Chemokine CCL5; D016328:NF-kappa B; C468119:NF-kappaB decoy; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides