Biphasic mini-reactor for characterization of biocatalyst performance.
Biphasic reaction media are extending the scope of technical biocatalysis. Thorough investigation of the factors affecting catalyst performance under these conditions is of key importance for the successful implementation of catalytic processes. Here, we present a reactor setup suitable for comprehensive systematic characterization and optimization of biocatalyzed reactions in biphasic systems with distinct phases. It is distinguished by small volumes allowing reproducible experimentation with minimum amounts of solvent and catalyst. The interfacial area is kept constant and independent stirring of both phases is allowed in order to minimize superimposing effects. Evaporation of low-volatile organic solvents is prevented by use of airtight construction. The broad applicability of this mini-reactor is demonstrated with regard to determination of mass transfer, enzyme productivity, and enzyme stability in both batch and continuous mode.
van den Wittenboer
Anne
A
Department of Biotechnology, RWTH Aachen University, Aachen, Germany.
Schmidt
Thomas
T
Müller
Pia
P
Ansorge-Schumacher
Marion B
MB
Greiner
Lasse
L
D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism; D001573:Benzoin / Q000378:metabolism; D055162:Biocatalysis; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation*; D002175:Candida / Q000235:genetics; D004795:Enzyme Stability; D004867:Equipment Design / Q000295:instrumentation; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D052196:Levilactobacillus brevis / Q000235:genetics; D008738:Methyl Ethers / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012997:Solvents / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D001547:Benzaldehydes; D008738:Methyl Ethers; D011994:Recombinant Proteins; D012997:Solvents; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D000446:Aldehyde-Lyases; C059416:benzaldehyde lyase; D001573:Benzoin