Effect of Piper betle on plasma antioxidant status and lipid profile against D-galactosamine-induced hepatitis in rats.
Betle leaf chewing is an old traditional practice in India and other countries of East Asia. We have investigated the antioxidant and antihyperlipidaemic potential of an alcoholic leaf-extract of Piper betle against D-galactosamine (D-GalN; 400 mg/kg body weight, i.p. single dose) intoxication in male albino Wistar rats. Rats were treated with leaf-extract (200 mg/kg body weight) by intragastric intubations daily for 20 days. The animals were divided randomly into five groups of six animals each as control, control plus extract, D-GalN control, D-GalN-rats on treatment with extract or silymarin, a standard drug. We observed an increase in the plasma levels of thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides, and a decrease in vitamin C, vitamin E and reduced glutathione concentrations. Very low density lipoprotein cholesterol and low density lipoprotein cholesterol increased significantly while high density lipoprotein cholesterol decreased. Further, increase in the levels of total cholesterol, phospholipids, triglycerides, free fatty acids in the plasma and tissues of liver and kidney were observed in D-GalN-treated rats. Administration of P. betle leaf-extract prevented the increase or decrease of these parameters and brought towards normality. These results suggest that P. betle could afford a significant antioxidant and antihyperlipidaemic effect against D-GalN-intoxication.
Pushpavalli
Ganesan
G
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamilnadu, India.
Veeramani
Chinnadurai
C
Pugalendi
Kodukkur Viswanathan
KV
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D005688:Galactosamine / Q000008:administration & dosage / Q000633:toxicity*; D005978:Glutathione / Q000097:blood; D007274:Injections, Intraperitoneal; D008055:Lipids / Q000097:blood*; D008297:Male; D031721:Piper betle / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008055:Lipids; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D005688:Galactosamine; D005978:Glutathione; D001205:Ascorbic Acid