title: [Optimal extraction of nuciferine and flavone from lotus leaf based on central composite design and response surface methodology].
abstract: OBJECTIVE
  To optimize the process of extracting effective constituents from lotus leaf.
  
  
  METHODS
  Independent variables were ethanol concentration reflux time and solvent fold, dependent variables were extraction rates of nuciferine and flavone in lotus leaf, central composite design and response surface methodology were used for optimization of extraction of lotus leaf.
  
  
  RESULTS
  The optimum conditions of extraction process were 75% -80% ethanol, 2-3 hours for reflux, 20-25 fold solvent and 2 times for extraction. Bias between observed and predicted of rates of nuciferine and flavone values were 5.53%, -6.02%, respectively.
  
  
  CONCLUSIONS
  The values observed and predicted were close to each other, which proved that the optimization of of extraction of lotus leaf by central composite design and response surface methodology was reasonable and successful.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Wang
authors.forename: Bao-Xian
authors.initials: BX
authors.lastname: Zhang
authors.forename: Qi-Xia
authors.initials: QX
authors.lastname: Nie
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Zang
mesh_terms: D001060:Aporphines / Q000737:chemistry / Q000302:isolation & purification*; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D000070116:Lotus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D001060:Aporphines; D047309:Flavones; D005419:Flavonoids; C043562:flavone; C008692:nuciferine