Herpesviruses in abscesses and cellulitis of endodontic origin.
Acute apical abscesses and cellulitis are severe endodontic diseases caused by opportunistic bacteria with possible coinfection with latent herpesviruses. The objectives of this study are to identify herpesviruses, including human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), herpes simplex virus-1 (HSV-1), and Varicella zoster virus (VZV) in patients (n = 31) presenting with acute apical abscesses and cellulitis of endodontic origin. Primary and nested polymerase chain reaction (PCR) was conducted using virus-specific primers and DNA isolated from cell-free abscess fluid. From patients exhibiting concurrent spontaneous pain (n = 28), nine abscesses contained HCMV, two abscesses contained EBV, one abscess contained HSV-1, and no abscesses contained VZV. Control PCR using genomic or recombinant templates showed detection limits to a single genomic copy of HCMV, 100 genomic copies for EBV, and 1 to 10 copies for HSV-1 with no cross-amplification between herpesviral DNA targets. Nested PCR was required for detection of herpesviral DNA in the abscess specimens, indicating that these viruses were present in low copy number. Filtration of abscess specimens and virus transfer experiments using human fibroblastic MRC-5 cells confirmed the presence of HCMV particles in several abscess specimens. We conclude that herpesviruses are present but not required for the development of acute apical abscesses and cellulitis of endodontic origin.
Chen
Vicky
V
Department of Integrative Biosciences, Oregon Health & Science University School of Dentistry, Portland, Oregon 97239, USA.
Chen
Yanwen
Y
Li
Hong
H
Kent
Karla
K
Baumgartner
J Craig
JC
Machida
Curtis A
CA
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016301:Alveolar Bone Loss / Q000821:virology; D002481:Cellulitis / Q000821:virology; D003587:Cytomegalovirus / Q000302:isolation & purification / Q000472:pathogenicity; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification / Q000472:pathogenicity; D004854:Herpesvirus 4, Human / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D010482:Periapical Abscess / Q000821:virology; D010483:Periapical Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction; D014098:Toothache / Q000821:virology; D014771:Virion / Q000302:isolation & purification; D055815:Young Adult
D004279:DNA, Viral