A novel medium for the production of cephamycin C by Nocardia lactamdurans using solid-state fermentation.
In this study, Nocardia lactamdurans NRRL 3802 was explored for the first time for production of cephamycin C by using solid-state fermentation. The effects of various substrates, moisture content, inoculum size, initial pH of culture medium, additional nitrogen source and amino acids were investigated for the maximum production of cephamycin C by N. lactamdurans NRRL 3802 in solid-state fermentation. Subsequently, selected fermentation parameters were further optimized by response surface methodology (RSM). The soybean flour as a substrate with moisture content of 65%, initial pH of culture medium of 6.5 and inoculum size of 10(9)CFU/ml (2 x 10(8)CFU/gds) at 28+/-2 degrees C after 4 days gave maximum production of 15.75+/-0.27 mg/gds of cephamycin C as compared to 8.37+/-0.23 mg/gds before optimization. Effect of 1,3-diaminopropane on cephamycin C production was further studied, which further increased the yield to 27.64+/-0.33 mg/gds.
Kagliwal
L D
LD
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400019, India.
Survase
S A
SA
Singhal
R S
RS
D000596:Amino Acids / Q000378:metabolism; D001431:Bacteriological Techniques; D018533:Biomass; D002513:Cephamycins / Q000096:biosynthesis*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D003959:Diamines / Q000494:pharmacology; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D009615:Nocardia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D000596:Amino Acids; D002513:Cephamycins; D003470:Culture Media; D003959:Diamines; C009475:trimethylenediamine; D009584:Nitrogen; C024925:cephamycin C