Monte Carlo dosimetry for targeted irradiation of individual cells using a microbeam facility.
Microbeam facilities provide a unique opportunity to investigate the effects of ionising radiation on living biological cells with a precise control of the delivered dose. This paper describes dosimetry calculations performed at the single-cell level in the microbeam irradiation facility available at the Centre d'Etudes Nucléaires de Bordeaux-Gradignan in France, using the object-oriented Geant4 Monte Carlo simulation toolkit. The cell geometry model is based on high-resolution three-dimensional voxelised phantoms of a human keratinocyte (HaCaT) cell line. Such phantoms are built from confocal microscopy imaging and from ion beam chemical elemental analysis. Results are presented for single-cell irradiation with 3 MeV incident alpha particles.
Incerti
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CNRS/IN2P3, Centre d'Etudes Nucléaires de Bordeaux-Gradignan, UMR 5797, Gradignan F-33175, France. incerti@cenbg.in2p3.fr
Seznec
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H
Simon
M
M
Barberet
Ph
P
Habchi
C
C
Moretto
Ph
P
D000465:Algorithms; D002468:Cell Physiological Phenomena / Q000528:radiation effects*; D003198:Computer Simulation; D006801:Humans; D008954:Models, Biological; D015233:Models, Statistical; D009010:Monte Carlo Method; D011874:Radiometry / Q000379:methods*; D011879:Radiotherapy Dosage; D011880:Radiotherapy Planning, Computer-Assisted / Q000379:methods*; D020266:Radiotherapy, Conformal / Q000379:methods*