title: Longitudinal diffusion tensor magnetic resonance imaging study of radiation-induced white matter damage in a rat model.
abstract: Radiation-induced white matter (WM) damage is a major side effect of whole brain irradiation among childhood cancer survivors. We evaluate longitudinally the diffusion characteristics of the late radiation-induced WM damage in a rat model after 25 and 30 Gy irradiation to the hemibrain at 8 time points from 2 to 48 weeks postradiation. We hypothesize that diffusion tensor magnetic resonance imaging (DTI) indices including fractional anisotropy (FA), trace, axial diffusivity (lambda(//)), and radial diffusivity (lambda( perpendicular)) can accurately detect and monitor the histopathologic changes of radiation-induced WM damage, measured at the EC, and that these changes are dose and time dependent. Results showed a progressive reduction of FA, which was driven by reduction in lambda(//) from 4 to 40 weeks postradiation, and an increase in lambda( perpendicular) with return to baseline in lambda(//) at 48 weeks postradiation. Histologic evaluation of irradiated WM showed reactive astrogliosis from 4 weeks postradiation with reversal at 36 weeks, and demyelination, axonal degeneration, and necrosis at 48 weeks postradiation. Moreover, changes in lambda(//) correlated with reactive astrogliosis (P < 0.01) and lambda( perpendicular) correlated with demyelination (P < 0.01). Higher radiation dose (30 Gy) induced earlier and more severe histologic changes than lower radiation dose (25 Gy), and these differences were reflected by the magnitude of changes in lambda(//) and lambda( perpendicular). DTI indices reflected the histopathologic changes of WM damage and our results support the use of DTI as a biomarker to noninvasively monitor radiation-induced WM damage.
authors.affiliation: Department of Diagnostic Radiology, The University of Hong Kong, Hong Kong.
authors.forename: Silun
authors.initials: S
authors.lastname: Wang
authors.forename: Ed X
authors.initials: EX
authors.lastname: Wu
authors.forename: Deqiang
authors.initials: D
authors.lastname: Qiu
authors.forename: Lucullus H T
authors.initials: LH
authors.lastname: Leung
authors.forename: Ho-Fai
authors.initials: HF
authors.lastname: Lau
authors.forename: Pek-Lan
authors.initials: PL
authors.lastname: Khong
mesh_terms: D000818:Animals; D001927:Brain Diseases / Q000209:etiology* / Q000473:pathology*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging / Q000379:methods; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012249:Rhombencephalon / Q000528:radiation effects*