title: Angiotensin-converting enzyme (ACE), angiotensinogen (AGT), and angiotensin II type 1 receptor (AT1R) gene polymorphisms in generalized aggressive periodontitis.
abstract: OBJECTIVE
  Host response to periodontopathic microorganisms can be modulated by genetic factors. Accumulated evidence highlighted the role of renin-angiotensin system (RAS) in inflammatory response thus potential implication of this molecular system in the pathogenesis of periodontitis can be suggested. The present study investigated common genetic variants of molecules within the RAS family namely angiotensin-converting enzyme (ACE), angiotensinogen (AGT) and angiotensin II type 1 receptor (AT1R) in relation to generalized aggressive periodontitis (G-AgP).
  
  
  METHODS
  DNA was obtained from peripheral blood of 103 G-AgP patients and 100 periodontally healthy subjects. ACE I/D, AGT M235T and AT1R A1166C polymorphisms were genotyped by polymerase chain reaction and restriction fragment length polymorphism method. Chi-square, ANOVA and logistic regression were used in statistical analyses.
  
  
  RESULTS
  Both ACE I/D and AT1R polymorphisms were similar in G-AgP and healthy groups (p>0.05). G-AgP subjects exhibited decreased AGT TT genotype and T allele frequency as compared to healthy subjects (p<0.05). The same trend was also observed in the nonsmoker subgroup regarding investigated RAS polymorphisms.
  
  
  CONCLUSIONS
  Present findings suggest that AGT M235T TT genotype and T allele might be associated with decreased risk for G-AgP in Turkish population.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Bornova-35100, Izmir, Turkey. ali.gurkan@ege.edu.tr
authors.forename: Ali
authors.initials: A
authors.lastname: Gürkan
authors.forename: Gülnur
authors.initials: G
authors.lastname: Emingil
authors.forename: Buket Han
authors.initials: BH
authors.lastname: Saygan
authors.forename: Gül
authors.initials: G
authors.lastname: Atilla
authors.forename: Timur
authors.initials: T
authors.lastname: Köse
authors.forename: Haluk
authors.initials: H
authors.lastname: Baylas
authors.forename: Afig
authors.initials: A
authors.lastname: Berdeli
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics* / Q000473:pathology; D000808:Angiotensinogen / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D018570:Risk Assessment; D012307:Risk Factors; D014421:Turkey; D055815:Young Adult
chemical_list: D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; D007703:Peptidyl-Dipeptidase A