Adzuki bean: a new resource of biocatalyst for asymmetric reduction of aromatic ketones with high stereoselectivity and substrate tolerance.
A new resource of biocatalyst for asymmetric reduction of aromatic ketones has been discovered for the first time from a common plant seed, adzuki bean, i.e. Phaseolus angularis (Willd.) W.F. Wight. The study investigated the best methods to prepare the biocatalyst and its ability to reduce ketones. Our results indicated that the biocatalyst from adzuki bean could reduce various aromatic ketones at relatively high concentrations (e.g. 100mM), exhibiting excellent stereoselectivity (>98% e.e.). In addition, it was found that NADPH acts as the reducing cofactor, which can be regenerated by the crude enzyme system itself using glucose as an auxiliary substrate.
Xie
Yan
Y
Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Xu
Jian-He
JH
Lu
Wen-Ya
WY
Lin
Guo-Qiang
GQ
D001711:Biotransformation; D002384:Catalysis; D003067:Coenzymes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D007659:Ketones / Q000737:chemistry*; D007700:Kinetics; D009249:NADP / Q000737:chemistry; D027805:Phaseolus / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000201:enzymology*; D013379:Substrate Specificity
D003067:Coenzymes; D007659:Ketones; D010936:Plant Extracts; D009249:NADP; D005947:Glucose