Detection of specific bioparticles using antibodies-coated gold nanoparticles and dielectrophoretic impedance measurement.
In this study, we developed an easy and quick test capable of identifying specific bacteria in one hour. The protocol was established based on the measurement of bacteria quantity on a biochip with comb-like electrodes. Gold nanoparticles coated with anti-Salmonella antibody were used to enhance the dielectrophretic property of Salmonella spp. on the biochip to facilitate the sensitivity.
Huang
Jung-Tang
JT
Department of Mechanical Engineering, National Taipei University of Technology, Taipei, Taiwan ROC. jthuang@ntut.edu.tw
Yu
Hua-Wei
HW
Hou
Shao-Yi
SY
Fang
Shiuh-Bin
SB
Lee
Hung-Chan
HC
Yang
Chu-Zen
CZ
D000465:Algorithms; D000906:Antibodies / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012475:Salmonella / Q000378:metabolism; D013234:Stem Cells; D013997:Time Factors
D000906:Antibodies; D006046:Gold