Influence of bone marrow graft B lymphocyte subsets on outcome after HLA-identical sibling transplants.
The potential role of the infused B cell subset after Hematopoietic Stem Cell Transplantation has not been yet studied. The present study analyzed the impact of B cells on transplant outcome in 254 patients who received a bone marrow graft from a human leucocyte antigen-identical sibling donor. The influence of B lineage-specific hematopoietic progenitor cells (CD34(+) CD19(+)) and B cells (immature and mature B cells, CD34(-) CD19(+)) was also analyzed. All included patients received a myeloablative regimen. The cumulative incidence function of acute graft-versus-host (GvHD) grade II to IV was 48% and was inversely associated with the number of CD34(+) CD19(+). There were no statistically significant associations between B cell subsets and chronic GvHD or survival. The CD34(+) CD19(+) B cell subset remained significantly associated with acute GvHD in multivariate analysis (Relative risk = 0.32, 95% confidence interval: 0.11-0.92, P = 0.035). In conclusion, a higher B lineage-specific hematopoietic progenitor cells (CD34(+) CD19(+)) cell dose is associated with a significant decrease incidence of acute GvHD.
Michonneau
David
D
Service d'Hématologie Greffe, Hôpital Saint Louis, Paris, France.
Peffault de Latour
Régis
R
Porcher
Raphaël
R
Robin
Marie
M
Benbunan
Marc
M
Rocha
Vanderson
V
Ribaud
Patricia
P
Ferry
Christele
C
Devergie
Agnes
A
Vanneaux
Valérie
V
Gluckman
Eliane
E
Marolleau
Jean Pierre
JP
Socié
Gérard
G
Larghero
Jérôme
J
D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000276:immunology*; D018952:Antigens, CD34 / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001855:Bone Marrow Diseases / Q000601:surgery; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000276:immunology; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D016016:Proportional Hazards Models; D012306:Risk; D035781:Siblings; D013234:Stem Cells / Q000276:immunology; D015996:Survival Rate; D014185:Transplantation, Isogeneic
D018941:Antigens, CD19; D018952:Antigens, CD34