title: System-wide molecular evidence for phenotypic buffering in Arabidopsis.
abstract: We profiled 162 lines of Arabidopsis for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects, suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs.
authors.affiliation: Groningen Bioinformatics Centre, University of Groningen, 9751NN Haren, The Netherlands.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Fu
authors.forename: Joost J B
authors.initials: JJ
authors.lastname: Keurentjes
authors.forename: Harro
authors.initials: H
authors.lastname: Bouwmeester
authors.forename: Twan
authors.initials: T
authors.lastname: America
authors.forename: Francel W A
authors.initials: FW
authors.lastname: Verstappen
authors.forename: Jane L
authors.initials: JL
authors.lastname: Ward
authors.forename: Michael H
authors.initials: MH
authors.lastname: Beale
authors.forename: Ric C H
authors.initials: RC
authors.lastname: de Vos
authors.forename: Martijn
authors.initials: M
authors.lastname: Dijkstra
authors.forename: Richard A
authors.initials: RA
authors.lastname: Scheltema
authors.forename: Frank
authors.initials: F
authors.lastname: Johannes
authors.forename: Maarten
authors.initials: M
authors.lastname: Koornneef
authors.forename: Dick
authors.initials: D
authors.lastname: Vreugdenhil
authors.forename: Rainer
authors.initials: R
authors.lastname: Breitling
authors.forename: Ritsert C
authors.initials: RC
authors.lastname: Jansen
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D002874:Chromosome Mapping; D020869:Gene Expression Profiling; D018745:Genome, Plant; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D015415:Biomarkers