title: Adhesion of Pseudomonas aeruginosa to orthokeratology and alignment lenses.
abstract: OBJECTIVE
  To determine whether contact lenses designed for orthokeratology (OK) are colonized by greater numbers of bacteria compared with standard (alignment fitted) design rigid gas permeable lenses before and after lens wear.
  
  
  METHODS
  Eighteen 1-year-old cats were randomly fitted with an OK lens in one eye and an alignment fitted (AF) lens in the other eye. Both lenses were made in the same diameter and central thickness and of the same material. Two separate wearing periods of 2 weeks and 6 weeks were used. After each wearing period, lenses were soaked in Pseudomonas aeruginosa (6294 or 6206) for 10 min. The lenses were then reinserted onto their respective corneas for a wearing period of 16 hours after which lenses were collected and remaining adhered bacteria quantified. Unworn control lenses were also soaked and bacteria enumerated for comparison.
  
  
  RESULTS
  There were no significant differences in the number of bacteria adherent to unworn AF and OK lenses. Analysis of lenses after wear showed OK lenses retained significantly higher numbers of viable bacteria than AF lenses in all studies.
  
  
  CONCLUSIONS
  OK lenses retain more bacteria than AF rigid gas permeable lenses after bacteria-loaded overnight lens wear. This may increase the risk for an infection in OK patients should suitable conditions be present. Specific education on the cleaning of OK lenses is essential.
authors.affiliation: Institute for Eye Research, Sydney, New South Wales, Australia. j.choo@ier.org.au
authors.forename: Jennifer D
authors.initials: JD
authors.lastname: Choo
authors.forename: Brien A
authors.initials: BA
authors.lastname: Holden
authors.forename: Eric B
authors.initials: EB
authors.lastname: Papas
authors.forename: Mark D P
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002415:Cats; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003262:Contact Lenses, Extended-Wear / Q000382:microbiology*; D004195:Disease Models, Animal; D015818:Eye Infections, Bacterial / Q000382:microbiology* / Q000517:prevention & control; D007634:Keratitis / Q000382:microbiology* / Q000517:prevention & control; D011552:Pseudomonas Infections / Q000382:microbiology* / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*
chemical_list: D018639:Contact Lens Solutions