A single residue in the 126-kDa protein of pepper mild mottle virus controls the severity of symptoms on infected green bell pepper plants.
Infectious cDNA clones originally derived from a mild strain of Pepper mild mottle virus were constructed by replacing residue 649, a critical point for attenuation of this virus, with all possible amino acids. All clones were infectious to pepper plants and induced a variety of symptoms, including no visible symptoms. The results of this study showed that a single amino acid mutation at residue 649 could control the function of the 126- and 183-kDa proteins, replicases with multiple roles in the life cycle of this virus.
Ichiki
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Research Team for Vectorborne Diseases, National Agricultural Research Center, 3-1-1 Kan-nondai, Tsukuba, Ibaraki, 305-8666, Japan. tuehara@affrc.go.jp
Nagaoka
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Hagiwara
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Sasaya
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Omura
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D019943:Amino Acid Substitution / Q000235:genetics; D002212:Capsicum / Q000821:virology*; D016297:Mutagenesis, Site-Directed; D020125:Mutation, Missense; D010935:Plant Diseases / Q000821:virology*; D017898:Tobamovirus / Q000235:genetics* / Q000472:pathogenicity*; D015678:Viral Structural Proteins / Q000235:genetics / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
D015678:Viral Structural Proteins; D037521:Virulence Factors