The surface display of the alginate lyase on the cells of Yarrowia lipolytica for hydrolysis of alginate.
The alginate lyase structural gene (AlyVI gene) was amplified from plasmid pET24-ALYVI carrying the alginate lyase gene from the marine bacterium Vibrio sp. QY101 which is a pathogen of Laminaria sp. When the gene was cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica, the cells displaying the alginate lyase could form clear zone on the plate containing sodium alginate, indicating that they had high alginate lyase activity. The cells displaying alginate lyase can be used to hydrolyze poly-beta-D: -mannuronate (M) and poly-alpha-L: -guluronate (G) and sodium alginate to produce different lengths of oligosaccharides (more than pentasaccharides). This is the first report that the yeast cells displaying alginate lyase were used to produce different lengths of oligosaccharides from alginate.
Liu
Guanglei
G
Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
Yue
Lixi
L
Chi
Zhe
Z
Yu
Wengong
W
Chi
Zhenming
Z
Madzak
Catherine
C
D000464:Alginates / Q000378:metabolism*; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008565:Membrane Proteins / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D013696:Temperature; D025062:Yarrowia / Q000201:enzymology*
D000464:Alginates; D006603:Hexuronic Acids; D008565:Membrane Proteins; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase