title: Limited efficacy of hydroxyurea in lowering of the JAK2 V617F allele burden.
abstract: Besides being an invaluable marker of clonal disease in chronic myeloproliferative disorders (CMPDs), the JAK2 V617F mutation and the mutated allele burden have an impact on disease phenotype and may provide information on prognosis. Recently, hydroxyurea (HU) has been shown to induce a rapid decline in the JAK2 V617F allele burden. The aim of the present study was to assess the dynamics of the JAK2 V617F allele burden during long-term treatment with HU in a series of patients with CMPDs. The JAK2 V617F allele burden was determined by quantitative PCR in 24 patients of whom 17 received HU, four received anagrelide and three were followed without any cytoreductive therapy. During a median follow-up of 24.2 months, no significant reductions in the JAK2 V617F allele burden were seen in patients treated with HU. We conclude that HU has only a limited effect on the JAK2 V617F allele burden in CMPD.
authors.affiliation: Department of Hematology, Odense University Hospital, Odense, Denmark. thomas.stauffer.larsen@ouh.regionsyddanmark.dk
authors.forename: Thomas Stauffer
authors.initials: TS
authors.lastname: Larsen
authors.forename: Niels
authors.initials: N
authors.lastname: Pallisgaard
authors.forename: Karin
authors.initials: K
authors.lastname: de Stricker
authors.forename: Michael Boe
authors.initials: MB
authors.lastname: Møller
authors.forename: Hans Carl
authors.initials: HC
authors.lastname: Hasselbalch
mesh_terms: D000483:Alleles; D002908:Chronic Disease; D005260:Female; D006801:Humans; D006918:Hydroxyurea / Q000627:therapeutic use*; D053614:Janus Kinase 2 / Q000235:genetics*; D008297:Male; D009154:Mutation / Q000187:drug effects*; D009196:Myeloproliferative Disorders / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics*; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D011799:Quinazolines / Q000627:therapeutic use*
chemical_list: D010975:Platelet Aggregation Inhibitors; D011799:Quinazolines; D053614:Janus Kinase 2; C021139:anagrelide; D006918:Hydroxyurea