Visualization of Src activity at different compartments of the plasma membrane by FRET imaging.
Membrane compartments function as segregated signaling platforms for different cellular functions. It is not clear how Src is regulated at different membrane compartments. To visualize local Src activity in live cells, a FRET-based Src biosensor was targeted in or outside of lipid rafts at the plasma membrane, via acylation or prenylation modifications on targeting tags either directly fused to the biosensor or coupled to the biosensor through an inducible heterodimerization system. In response to growth factors and pervanadate, the induction of Src activity in rafts was slower and weaker, dependent on actin and possibly its mediated transportation of Src from perinuclear regions to the plasma membrane. In contrast, the induction of Src activity in nonrafts was faster and stronger, dependent on microtubules. Hence, Src activity is differentially regulated via cytoskeleton at different membrane compartments.
Seong
Jihye
J
Neuroscience Program, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Lu
Shaoying
S
Ouyang
Mingxing
M
Huang
He
H
Zhang
Jin
J
Frame
Margaret C
MC
Wang
Yingxiao
Y
D000199:Actins / Q000378:metabolism; D015374:Biosensing Techniques; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D021381:Protein Transport; D014638:Vanadates / Q000494:pharmacology; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000199:Actins; D010982:Platelet-Derived Growth Factor; C076715:pervanadate; D014638:Vanadates; D004815:Epidermal Growth Factor; D019061:src-Family Kinases