Optimization and comparison of five methods for extraction of coniferyl ferulate from Angelica sinensis.
Coniferyl ferulate, which is noted for its multiple pharmacological activities and chemical instability, is abundant in Angelica sinensis. In this paper, five methods, namely sonication extraction (SE), pressurized liquid extraction (PLE), supercritical fluid extraction (SFE), hydrodistillation (HD) and decoction (DC) for extraction of coniferyl ferulate, as well as ferulic acid, Z/E-ligustilide and Z/E-butylidenephthalide, from A. sinensis were optimized and compared. The results showed that the order of extraction efficiency was: PLE>>SE>SFE>>HD, DC. The compositions of the SE, PLE and SFE extracts, which had a high ratio of coniferyl ferulate, were very similar, while no coniferyl ferulate was obtained by HD and DC, though they had high selectivity for the extraction of ligustilide and ferulic acid, respectively. It was noteworthy that the content of ligustilide and coniferyl ferulate was not detectable in the decoction, the commonly used oral administration form of Traditional Chinese Medicines in clinical practice.
Xie
Jing-Jing
JJ
Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
Lu
Jia
J
Qian
Zheng-Ming
ZM
Yu
Yue
Y
Duan
Jin-Ao
JA
Li
Shao-Ping
SP
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis; D029971:Angelica sinensis / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis*; D013010:Sonication
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C044571:coniferyl ferulate; C027820:ligustilide; D015107:4-Butyrolactone