Expression profiles of three novel sensory guanylate cyclases and guanylate cyclase-activating proteins in the zebrafish retina.
Three membrane bound sensory guanylate cyclases are expressed in photoreceptor cells of the developing and adult zebrafish retina. First appearance of mRNA transcripts was detected by in situ hybridization techniques for all guanylate cyclases between 3 and 4 days post fertilization (dpf), but only one isoform (guanylate cyclase 3) appeared to be specifically expressed in cones of the adult retina. Transcripts of three cone specific guanylate cyclase-activating proteins (zGCAP3, zGCAP4 and zGCAP7) were also detected at 3-4 dpf. The expression onset of the guanylate cyclases and these neuronal calcium sensor proteins mainly overlapped. High guanylate cyclase activities in larval eye preparations and the precisely controlled coexpression of guanylate cyclases and zGCAPs coincide with the onset of visual function at 3-4 dpf.
Rätscho
Nina
N
Biochemistry Group, Institute of Biology and Environmental Science, Faculty V, Carl von Ossietzky University Oldenburg, D-26111 Oldenburg, Germany.
Scholten
Alexander
A
Koch
Karl-Wilhelm
KW
D000818:Animals; D020869:Gene Expression Profiling; D006162:Guanylate Cyclase / Q000235:genetics / Q000378:metabolism*; D051604:Guanylate Cyclase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000033:anatomy & histology / Q000378:metabolism; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D051604:Guanylate Cyclase-Activating Proteins; D020033:Protein Isoforms; D029961:Zebrafish Proteins; C496743:guca1c protein, zebrafish; C499851:guca1d protein, zebrafish; C499853:guca1g protein, zebrafish; D006162:Guanylate Cyclase