title: [Studies on chemical constituents of Ranunculus japonicus].
abstract: OBJECTIVE
  To isolate and determine the chemical constituents of Ranunculus japonicus in Liupan Mountain, Ningxia province, China.
  
  
  METHODS
  The herb was extracted with ethanol by ultrasonic bath. The extractives were divided to petroleum ether, ethyl acetate, n-butanol parts. The first two parts were separated and purified with silica gel and Sephadex LH -20 column chromatography. The structures of the separated compounds were idnetefied by physical and chemical properties and spectral analysis.
  
  
  RESULTS
  Nine compounds were isolated and identified as follows: scoparone (1), tricin (2), protocatechuic acid (3), luteolin (4), anemonin (5), scopoletin (6), 5-hydroxy-6, 7-dimethoxyflavone (7), ternatolide (8), 5-hydroxy-7, 8-dimethoxy-flavone (9).
  
  
  CONCLUSIONS
  Compounds 1-9 were isolated from Ranunculus japonicus for the first time.
authors.affiliation: College of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, China. qylyf338@yahoo.com.cn
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Liang
authors.forename: Zuo-Tao
authors.initials: ZT
authors.lastname: Chen
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Liu
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000473:Alkanes / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry; D005663:Furans / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry; D047311:Luteolin / Q000737:chemistry; D031947:Ranunculus / Q000737:chemistry*; D012603:Scopoletin / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000085:Acetates; D000473:Alkanes; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D005663:Furans; D062385:Hydroxybenzoates; C009091:protocatechuic acid; D000431:Ethanol; C007650:ethyl acetate; D020001:1-Butanol; C017769:tricin; C028304:anemonin; C018145:scoparone; D012603:Scopoletin; D047311:Luteolin; C004544:naphtha; C043562:flavone