title: A bright and photostable photoconvertible fluorescent protein.
abstract: Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent.
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, Virginia 20147, USA.
authors.forename: Sean A
authors.initials: SA
authors.lastname: McKinney
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Murphy
authors.forename: Kristin L
authors.initials: KL
authors.lastname: Hazelwood
authors.forename: Michael W
authors.initials: MW
authors.lastname: Davidson
authors.forename: Loren L
authors.initials: LL
authors.lastname: Looger
mesh_terms: D000818:Animals; D005347:Fibroblasts; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods; D016297:Mutagenesis, Site-Directed; D055668:Photochemical Processes; D011993:Recombinant Fusion Proteins / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins