title: Hodgkin lymphoma cell lines are characterized by a specific miRNA expression profile.
abstract: Hodgkin lymphoma (HL) is derived from preapoptotic germinal center B cells, although a general loss of B cell phenotype is noted. Using quantitative reverse transcription-polymerase chain reaction and miRNA microarray, we determined the microRNA (miRNA) profile of HL and compared this with the profile of a panel of B-cell non-Hodgkin lymphomas. The two methods showed a strong correlation for the detection of miRNA expression levels. The HL-specific miRNA included miR-17-92 cluster members, miR-16, miR-21, miR-24, and miR-155. Using a large panel of cell lines, we found differential expression between HL and other B-cell lymphoma-derived cell lines for 27 miRNA. A significant down-regulation in HL compared to non-Hodgkin lymphoma was observed only for miR-150. Next, we performed target gene validation of predicted target genes for miR-155, which is highly expressed in HL and is differentially expressed between HL and Burkitt lymphoma. Using luciferase reporter assays, we validated 11 predicted miR-155 target genes in three different HL cell lines. We demonstrated that AGTR1, FGF7, ZNF537, ZIC3, and IKBKE are true miR-155 target genes in HL.
authors.affiliation: Department Pathology & Laboratory Medicine, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands.
authors.forename: Johan H
authors.initials: JH
authors.lastname: Gibcus
authors.forename: Lu Ping
authors.initials: LP
authors.lastname: Tan
authors.forename: Geert
authors.initials: G
authors.lastname: Harms
authors.forename: Rikst Nynke
authors.initials: RN
authors.lastname: Schakel
authors.forename: Debora
authors.initials: D
authors.lastname: de Jong
authors.forename: Tjasso
authors.initials: T
authors.lastname: Blokzijl
authors.forename: Peter
authors.initials: P
authors.lastname: Möller
authors.forename: Sibrand
authors.initials: S
authors.lastname: Poppema
authors.forename: Bart-Jan
authors.initials: BJ
authors.lastname: Kroesen
authors.forename: Anke
authors.initials: A
authors.lastname: van den Berg
mesh_terms: D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs