title: Studies on mefenamic acid microparticles: formulation, in vitro release, and in situ studies in rats.
abstract: In this study, we investigated the in vitro characteristics of mefenamic acid (MA) microparticles as well as their effects on DNA damage. MA-loaded chitosan and alginate beads were prepared by the ionotropic gelation process. Microsponges containing MA and Eudragit RS 100 were prepared by quasi-emulsion solvent diffusion method. The microparticles were characterized in terms of particle size, surface morphology, encapsulation efficiency, and in vitro release profiles. Most of the formulation variables manifested an influence on the physical characteristics of the microparticles at varying degrees. We also studied the effects of MA, MA-loaded microparticles, and three different polymers on rat brain cortex DNA damage. Our results showed that DNA damage was higher in MA-loaded Eudragit microsponges than MA-loaded biodegradable chitosan or alginate microparticles.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey, ferhan.sevgi@ege.edu.tr.
authors.forename: Ferhan
authors.initials: F
authors.lastname: Sevgi
authors.forename: Aysu
authors.initials: A
authors.lastname: Yurdasiper
authors.forename: Buket
authors.initials: B
authors.lastname: Kaynarsoy
authors.forename: Ezgi
authors.initials: E
authors.lastname: Turunç
authors.forename: Tamer
authors.initials: T
authors.lastname: Güneri
authors.forename: Ayfer
authors.initials: A
authors.lastname: Yalçin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000464:Alginates / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000473:pathology; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry; D004249:DNA Damage; D004304:Dosage Forms; D004337:Drug Carriers; D004339:Drug Compounding; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007608:Kainic Acid / Q000633:toxicity; D007700:Kinetics; D008528:Mefenamic Acid / Q000737:chemistry / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000180:Acrylic Resins; D000464:Alginates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004304:Dosage Forms; D004337:Drug Carriers; D018690:Excitatory Amino Acid Agonists; D006603:Hexuronic Acids; D018696:Neuroprotective Agents; C050528:Eudragit RS; D008528:Mefenamic Acid; D020723:Glucuronic Acid; D048271:Chitosan; D007608:Kainic Acid