[Effect of selenium-protein polysaccharide extracted from Se-rich Cordyceps militaris on tumor-bearing mice].
OBJECTIVE
To extract and analysis the active components of Se-protein polysaccharide from Se-rich Cordyceps militaris, and to discuss the anti-tumor effect of Se-protein polysaccharide.


METHODS
Protein, polysaccharides and selenium content were determined by the methods of Folin-phenol reagent (lowry), phenol-sulfate and DAN fluorescence, respectively. Tumor-bearing mouse model was established and divided into the model group, cyclophosphamide group, cordyceps high and low dosage group (200, 100 mg x kg(-1)). Then the Se-protein polysaccharide's anti-tumor activity and immune function in vivo were observed by compare with model group in the weight of mice, inhibitory rate, conversion rate of peripheral blood lymphocytes, dissection index K, swallowed factor alpha, liver and spleen factor coefficient, GSH-Px and SOD activity and the content of MDA.


RESULTS
Se-protein polysaccharides from Se-rich Cordyceps militaris had a significant anti-tumor action with the inhibitory rate 46.92% and could avoid toxic effect of chemotherapy drug like cyclophosphamide. It also could enhance immune function and body antioxidant capacity by inhibiting the decline of tumor-bearing mouse liver coefficient and spleen coefficient in tumor-bearing mice caused by cyclophosphamide.


CONCLUSIONS
Se-protein polysaccharide, the extraction of Se-rich Cordyceps militaris's can inhibit tumor grouth of tumor-bearing mouse.
Zhong
Ming
M
Key Laboratory of Agriculture Biotechnology of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, China. zhming@syau.edu.cn
Wang
Li-He
LH
Ma
Hui
H
Guo
Zhi-Fu
ZF
Wang
Cheng-Chun
CC
Liu
Shao-Xia
SX
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D032661:Cordyceps / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005656:Fungal Proteins / Q000737:chemistry*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012643:Selenium / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000970:Antineoplastic Agents; D000975:Antioxidants; D005656:Fungal Proteins; D011134:Polysaccharides; D013482:Superoxide Dismutase; D012643:Selenium