Time-dependent inactivation of human phenylethanolamine N-methyltransferase by 7-isothiocyanatotetrahydroisoquinoline.
Inhibitors of phenylethanolamine N-methyltransferase [PNMT, the enzyme that catalyzes the final step in the biosynthesis of epinephrine (Epi)] may be of use in determining the role of Epi in the central nervous system. Here we describe the synthesis and characterization of 7-SCN tetrahydroisoquinoline as an affinity label for human PNMT.
Wu
Qian
Q
College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
Caine
Joanne M
JM
Thomson
Stuart A
SA
Slavica
Meri
M
Grunewald
Gary L
GL
McLeish
Michael J
MJ
D000818:Animals; D004837:Epinephrine / Q000378:metabolism / Q000502:physiology; D006801:Humans; D010625:Phenylethanolamine N-Methyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D044005:Tetrahydroisoquinolines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
C538959:7-isothiocyanatotetrahydroisoquinoline; D044005:Tetrahydroisoquinolines; D010625:Phenylethanolamine N-Methyltransferase; D004837:Epinephrine