Changes in circulating dendritic cells and B-cells in patients with multiple sclerosis relapse during corticosteroid therapy.
We studied dendritic, myeloid (mDCs) (BDCA1+/CD19-) and plasmacytoid cells (pDCs) (BDCA2+/CD123+) and B-cells (CD19+) at relapse, after short intravenous methyloprednisolone therapy and 30 days after treatment completion (remission period) and results were compared with a control group of healthy volunteers. We confirmed the decrease in BDCA2+/CD123+ after therapy (p=0.00006) and then an increase (p=0.0032) in comparison to the control group. The level of B CD19+ cells after GC therapy increased compared to the control group (p=0.00809) and had stabilised 30 days after therapy. The effect of methyloprednisolone on DC function and B-cell levels thus changes.
Krystyna
Mitosek-Szewczyk
MS
Department of Neurology, Medical University in Lublin, ul. Jaczewskiego 8, 20-090 Lublin, Poland. krystyna.mitosek@am.lublin.pl
Jacek
Tabarkiewicz
T
Sebastian
Radej
R
Ewa
Belniak
B
Halina
Bartosik-Psujek
BP
Zbigniew
Stelmasiak
S
Jacek
Rolinski
R
D000305:Adrenal Cortex Hormones / Q000008:administration & dosage*; D000328:Adult; D018949:Antigens, CD1; D018941:Antigens, CD19 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D001402:B-Lymphocytes / Q000187:drug effects*; D003713:Dendritic Cells / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006023:Glycoproteins; D006801:Humans; D016130:Immunophenotyping; D007275:Injections, Intravenous / Q000379:methods; D037181:Lectins, C-Type / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008775:Methylprednisolone / Q000008:administration & dosage*; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D011971:Receptors, Immunologic / Q000378:metabolism; D018709:Statistics, Nonparametric; D055815:Young Adult
D000305:Adrenal Cortex Hormones; D018949:Antigens, CD1; D018941:Antigens, CD19; D000954:Antigens, Surface; C437944:CD1C protein, human; C443299:CLEC4C protein, human; D006023:Glycoproteins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D008775:Methylprednisolone