Maternal MTHFR polymorphisms and risk of spontaneous abortion.
OBJECTIVE
To asses the association between intake of folate and B vitamins and the incidence of spontaneous abortion (SA) according to the maternal methylenetetrahydrofolate reductase (MTHFR) polymorphisms (677 C>T and 1298 A>C).


METHODS
We conducted a nested case-control study within a perinatal cohort of women recruited in the state of Morelos, Mexico. Twenty-three women with SA were compared to 74 women whose pregnancy survived beyond week 20th. Intake of folate and B vitamins respectively, was estimated using a validated food frequency questionnaire. Maternal MTHFR polymorphisms were determined by PCR-RFLP and serum homocysteine levels by HPLC.


RESULTS
Carriers of MTHFR 677TT and 1298AC genotypes respectively showed an increased risk of SA (OR 677TT vs. CC/CT=5.0; 95% CI: 1.2, 20.9 and OR 1298 AC vs. AA=5.5; 95% CI: 1.1, 26.6).


CONCLUSIONS
Our results support the role of MTHFR polymorphisms as a risk factor for SA, regardless of dietary intake of B vitamins.
Rodríguez-Guillén
María del Rosario
Mdel R
National Institute of Public Health, Morelos, México.
Torres-Sánchez
Luisa
L
Chen
Jia
J
Galván-Portillo
Marcia
M
Blanco-Muñoz
Julia
J
Anaya
Miriam Aracely
MA
Silva-Zolezzi
Irma
I
Hernández-Valero
María A
MA
López-Carrillo
Lizbeth
L
D000022:Abortion, Spontaneous / Q000453:epidemiology / Q000235:genetics*; D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D004034:Diet Surveys; D005260:Female; D005492:Folic Acid; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008800:Mexico / Q000453:epidemiology; D009790:Occupations / Q000706:statistics & numerical data; D018812:Paternal Exposure / Q000706:statistics & numerical data; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D012307:Risk Factors; D012907:Smoking / Q000453:epidemiology; D014803:Vitamin B Complex; D014931:Women, Working; D055815:Young Adult
D006710:Homocysteine; D014803:Vitamin B Complex; D005492:Folic Acid; D042965:Methylenetetrahydrofolate Reductase (NADPH2)