Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis.
We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
Oana
Kozue
K
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Japan.
Kawakami
Yoshiyuki
Y
Hayashi
Tetsuya
T
Ohnishi
Makoto
M
D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D005821:Genetic Techniques; D016680:Genome, Bacterial; D009113:Muramidase / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009113:Muramidase