title: [BRAF mutation in colorectal serrated lesions].
abstract: OBJECTIVE
  To investigate the viral oncogene homolog B1 (BRAF) mutation frequency in serrated colorectal adenocarcinoma (Sca), and to verify the existence of a new serrated pathway indirectly.
  
  
  METHODS
  75 paraffin-embedded tissue samples, including 15 cases of Sca, 20 cases of traditional serrated adenoma (TSA), 20 cases of non-serrated adenocarcinoma (NS-ca), and 20 cases of villous adenoma, were reviewed. Nest-PCR was used to amplify the exon 15 fragment of BRAF gene. RFLP assay was used to detect the BRAF V600E mutation. The positive samples were verified by direct sequencing.
  
  
  RESULTS
  Four of the 11 successfully amplified Sca specimens (4/11, 36.4%), and 4 of the 15 TSA samples (4/15, 26.7%) harbored BRAF V600E mutation. However, no BRAF V600E mutation was found in the 14 NS-ca samples and the 11 villous adenoma specimens. The BRAF V600E mutation frequencies of the villous adenoma group and NS-ca group were significantly lower than those of the TSA group and Sca group (P = 0.0084); however, there was no statistical difference in the frequency of BRAF V600E mutation between the Sca and TSA groups (P > 0.05).
  
  
  CONCLUSIONS
  BRAF V600E mutation frequency is significantly higher in the Sca and TSA patients than in the NS-ca and villous adenoma patients. There is actually a new serrated neoplasia pathway different from the traditional adenoma-carcinoma carcinogenesis pathway.
authors.affiliation: Department of Gastroenterology, Beijing Military Command's General Hospital, Beijing 100700, China.
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Lu-ping
authors.initials: LP
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018899:CpG Islands; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012189:Retrospective Studies
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf