title: Quantifying the development of the peripheral naive CD4+ T-cell pool in humans.
abstract: What are the rules that govern a naive T cell's prospects for survival or division after export from the thymus into the periphery? To help address these questions, we combine data from existing studies with robust mathematical models to estimate the absolute contributions of thymopoiesis, peripheral division, and loss or differentiation to the human naive CD4+ T-cell pool between the ages of 0 and 20 years. Despite their decline in frequency in the blood, total body numbers of naive CD4+ T cells increase throughout childhood and early adulthood. Our analysis shows that postthymic proliferation contributes more than double the number of cells entering the pool each day from the thymus. This ratio is preserved with age; as the thymus involutes, the average time between naive T-cell divisions in the periphery lengthens. We also show that the expected residence time of naive T cells increases with time. The naive CD4+ T-cell population thus becomes progressively less dynamic with age. Together with other studies, our results suggest a complex picture of naive T-cell homeostasis in which population size, time since export from the thymus, or time since the last division can influence a cell's prospects for survival or further divisions.
authors.affiliation: Immunobiology Unit, Institute of Child Health, London, United Kingdom.
authors.forename: Iren
authors.initials: I
authors.lastname: Bains
authors.forename: Rustom
authors.initials: R
authors.lastname: Antia
authors.forename: Robin
authors.initials: R
authors.lastname: Callard
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Yates
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging / Q000097:blood; D001692:Biological Transport; D001810:Blood Volume / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D018791:CD4 Lymphocyte Count / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008954:Models, Biological; D050378:T-Lymphocytes, Regulatory / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D055815:Young Adult