Early gene expression analyzed by a genome microarray and real-time PCR in osteoblasts cultured with a 4-META/MMA-TBB adhesive resin sealer.
OBJECTIVE
Adhesive resin sealer systems have been applied in endodontics to seal the root canal system. This study was designed to confirm the mechanism of intracellular molecular events in an in vitro cell culture system with a 4-methacryloxyethyl trimellitate anhydride/methylmethacrylate-tri-n-butyl borane (4-META/MMA-TBB) adhesive resin sealer.


METHODS
The gene expression patterns relating to cell growth and differentiation were examined using a human genome expression microarray and real-time polymerase chain reaction analyses in hard tissue-forming osteoblasts cultured with and without a 4-META/MMA-TBB resin sealer.


RESULTS
There was no significant difference in the cell number between the control and adhesive sealer groups. An increased expression of integrin beta, transforming growth factor beta-related protein, craniofacial development protein 1, and PI3K genes was demonstrated. The integrin beta and PI3K genes showed extremely high ratios.


CONCLUSIONS
The signal transduction pathway, at least through the PI3K/Akt cascade for cell proliferation and differentiation, can be controlled by some components of this type of adhesive resin sealer.
Syudo
Minoru
M
Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Yamada
Shizuka
S
Yanagiguchi
Kajiro
K
Matsunaga
Tsunenori
T
Hayashi
Yoshihiko
Y
D000180:Acrylic Resins / Q000494:pharmacology*; D045744:Cell Line, Tumor; D058541:Class II Phosphatidylinositol 3-Kinases; D006801:Humans; D039661:Integrin beta3 / Q000096:biosynthesis; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008422:Materials Testing; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000096:biosynthesis; D012387:Root Canal Filling Materials / Q000494:pharmacology*
D000180:Acrylic Resins; D058541:Class II Phosphatidylinositol 3-Kinases; D039661:Integrin beta3; D009687:Nuclear Proteins; D019869:Phosphatidylinositol 3-Kinases; D010750:Phosphoproteins; D012097:Repressor Proteins; D012387:Root Canal Filling Materials; C104422:CFDP1 protein, human; C099726:TSC22D1 protein, human; C031172:4-META resin