title: Rapid and sensitive LC-MS/MS assay for the quantitation of 20(S)-protopanaxadiol in human plasma.
abstract: This paper describes a rapid and sensitive method for the quantitation of 20(S)-protopanaxadiol (PPD) in human plasma based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte and internal standard (I.S.), ginsenoside Rh(2), were extracted from plasma by liquid-liquid extraction and separated on a Zorbax extend C(18) analytical column using methanol-acetonitrile-10mM ammonium acetate (47.5:47.5:5, v/v/v) as mobile phase. Detection was by tandem mass spectrometry using electrospray ionization in the positive ion mode and multiple reaction monitoring (MRM). The assay was linear over the concentration range 0.1-100.0ng/ml with a limit of detection of 0.05ng/ml. The method was successfully applied to a clinical pharmacokinetic study in healthy volunteers after a single oral administration of a PPD 25mg capsule.
authors.affiliation: Research Center for Drug Metabolism, Jilin University, Changchun 130023, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiangbin
authors.initials: J
authors.lastname: Han
authors.forename: Weisong
authors.initials: W
authors.lastname: Yu
authors.forename: Lili
authors.initials: L
authors.lastname: Deng
authors.forename: J Paul
authors.initials: JP
authors.lastname: Fawcett
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012502:Sapogenins / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D012502:Sapogenins; C062916:protopanaxadiol