Detection of carbonylated proteins in two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis separations.
Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins.
Sultana
Rukhsana
R
Department of Chemistry, Center of Membrane Sciences, University of Kentucky, Lexington, KY, USA.
Newman
Shelley F
SF
Huang
Quanzhen
Q
Butterfield
D Allan
DA
D000818:Animals; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D021621:Imaging, Three-Dimensional; D015151:Immunoblotting; D007525:Isoelectric Focusing; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
D011506:Proteins