title: Human alveolar bone-derived cell-culture behaviour on biodegradable poly(L-lactic Acid).
abstract: Poly(L-lactic acid) (PLA) is a polymer of great technological interest, whose excellent mechanical properties, thermal plasticity and bioresorbability render it potentially useful for environmental applications, as a biodegradable plastic and as a biocompatible material in biomedicine. The interactions between an implant material surface and host cells play central roles in the integration, biological performance and clinical success of implanted biomedical devices. Osteoblasts from human alveolar bone were chosen to investigate the cell behaviour when in contact with PLA discs. Cell morphology and adhesion through osteopontin (OPN) and fibronectin (FN) expression were evaluated in the initial osteogenesis, as well as cell proliferation, alkaline phosphatase activity and bone nodule formation. It was shown that the polymer favoured cell attachment. Cell proliferation increased until 21 days but in a smaller rate when compared to the control group. On the other hand, ALP activity and bone mineralization were not enhanced by the polymer. It is suggested that this polymer favours cell adhesion in the early osteogenesis in vitro, but it does not enhance differentiation and mineralization.
authors.affiliation: Department of Morphology, Stomatology and Physiology, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. do Café, s/n, 14040-904, Ribeirão Preto, SP, Brazil. karina@forp.usp.br
authors.forename: Karina Fittipaldi
authors.initials: KF
authors.lastname: Bombonato-Prado
authors.forename: Maidy Redher
authors.initials: MR
authors.lastname: Wimmers Ferreira
authors.forename: Adalberto Luiz
authors.initials: AL
authors.lastname: Rosa
authors.forename: Paulo Tambasco
authors.initials: PT
authors.lastname: de Oliveira
authors.forename: Vanusca Dalosto
authors.initials: VD
authors.lastname: Jahno
authors.forename: Jefferson Braga
authors.initials: JB
authors.lastname: da Silva
authors.forename: Rosane
authors.initials: R
authors.lastname: Ligabue
authors.forename: Sandra
authors.initials: S
authors.lastname: Einloft
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials; D001846:Bone Development; D002113:Calcification, Physiologic; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D048708:Cell Growth Processes / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology; D053495:Osteopontin / Q000378:metabolism; D011091:Polyesters
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D011091:Polyesters; D053495:Osteopontin; C033616:poly(lactide); D000469:Alkaline Phosphatase