title: [Establishment of human embryonic stem cell line NJGLLhES1].
abstract: OBJECTIVE
  To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1.
  
  
  METHODS
  From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1. We detected the karyotype of the cell line, determined the expressions of alkaline phosphatase, the specific cell surface antigens SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and the marker gene Oct-4, and examined the formation of embryoids and teratomas.
  
  
  RESULTS
  NJGLLhES1 was maintained for over 1 year in vitro, with the morphological characteristics of hESC, a normal karyotype, positive expressions of alkaline phosphatase and specific cell marker genes, and the potential of forming embryoids and teratomas.
  
  
  CONCLUSIONS
  A new human embryonic stem cell line NJGLLhES1 was successfully established, which remains karyotypically and phenotypically stable, undifferentiated and capable of self-renewal and pluripotential differentiation.
authors.affiliation: Center of Reproductive Medicine, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China. stevensunz@163.com
authors.forename: Hai-Xiang
authors.initials: HX
authors.lastname: Sun
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Hu
authors.forename: Ning-Yuan
authors.initials: NY
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Wang
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Xu
authors.forename: Lin-Jun
authors.initials: LJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR