CSF profiles of angiogenic and inflammatory factors depend on the respiratory status of ALS patients.
Our objective was to assess the regulation of the hypoxia response of angiogenic and inflammatory factors from 76 cerebrospinal fluids (CSF) of sporadic amyotrophic lateral sclerosis (ALS) patients with different respiratory status. We first analysed the hypoxia response capacity by measuring CSF levels of angiogenin (ANG), VEGF, angiopoietin-2 (ANG-2) and PGE-2 in 40 ALS patients according to their hypoxaemia level and compared it with 40 neurological controls. We then compared the ANG, VEGF, EPO and ANG-2 CSF levels of 36 other ALS patients, divided into three groups with either 1) normoxaemia, 2) intermittent desaturation in the absence of hypoxaemia, or 3) chronic hypoxaemia with or without desaturation. We demonstrated a lack of up-regulation of both ANG and VEGF during hypoxaemia in ALS, compared with hypoxaemic controls. In contrast, PGE-2 and ANG-2 levels were increased in both hypoxaemic ALS patients and controls. ANG and VEGF levels did not increase in patients with long disease durations and with intermittent or chronic hypoxaemia. ANG-2 and EPO levels were up-regulated early in intermittent hypoxaemia and late in chronic hypoxaemia, respectively. Our results suggest alteration of the HIF-1alpha-mediated response to hypoxia during sporadic ALS, whereas the NFK-B pathway seems early activated.
Moreau
Caroline
C
Service de Neurologie et Pathologie du Mouvement, Hôpital Roger Salengro, CHRU, Lille, France.
Gosset
Philippe
P
Brunaud-Danel
Véronique
V
Lassalle
Philippe
P
Degonne
Benoist
B
Destee
Alain
A
Defebvre
Luc
L
Devos
David
D
D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid*; D042702:Angiopoietin-2 / Q000134:cerebrospinal fluid; D015232:Dinoprostone / Q000134:cerebrospinal fluid; D004921:Erythropoietin / Q000134:cerebrospinal fluid; D006801:Humans; D000860:Hypoxia; D007249:Inflammation / Q000134:cerebrospinal fluid; D008875:Middle Aged; D010100:Oxygen / Q000097:blood; D012119:Respiration; D012259:Ribonuclease, Pancreatic / Q000134:cerebrospinal fluid; D042461:Vascular Endothelial Growth Factor A / Q000134:cerebrospinal fluid
D042702:Angiopoietin-2; D042461:Vascular Endothelial Growth Factor A; D004921:Erythropoietin; C046366:angiogenin; D012259:Ribonuclease, Pancreatic; D015232:Dinoprostone; D010100:Oxygen