[Isolation and identification of a thermophilic bacterium from hot springs in Changbai Mountains].
OBJECTIVE
We studied physiological and biochemical properties of a thermophilic bacterium isolated from hot springs in Changbai Mountain.


METHODS
The strain CBS-5 (=JCM 15484) from hot springs in Changbai Mountains was isolated by plating and screening on Olive-rich medium. It was characterized by the physiological and biochemical analysis, 16S rDNA sequencing and determination of (G+C)mol% contents.


RESULTS
The cells were Gram-positive, non-motile rods, spore-forming and generally occurred singly or in pairs. The growth temperature ranges from 48 degrees C to 70 degrees C, optimum at 65 degrees C; growth pH ranges from 6.5 to 8.5, optimum at 7.7. The strain used glucose, sucrose, maltose, lactose and rhanmose as carbon and energy sources. The G+C content of DNA was 41.9 mol%. The major cellular fatty acids were iso-C15:0, C16:0 and iso-C17:0, respectively representing about 24.20%, 20.45% and 17.42% of total fatty acids. The 16S rDNA sequence analysis showed that the strain belonged to the Anoxybacillus genus, for the sequence similarity was 95.1%-98.5% to other known species of Anoxybacillus.


CONCLUSIONS
Strain CBS-5 was a member of Anoxybacillus.
Liu
Donglai
D
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, China. ldl198653@126.com
Cai
Jingang
J
Yao
Tianyu
T
Wang
Yanping
Y
Kong
Qingyang
Q
Feng
Yan
Y
D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S