Effect of pH, EDTA, and anions on heavy metal toxicity toward a bioluminescent cyanobacterial bioreporter.
The bioavailability and therefore toxicity of a metal depends on the chemical species present in a particular environment. We evaluated the effect of a series of factors that could potentially modify metal speciation on the toxicity of Hg, Cu, Zn, and Cd toward a recombinant strain of the freshwater cyanobacterium Anabaena sp. PCC 7120 with cloned lux operon of luminescent terrestrial bacterium Photorhabdus luminescens. The strain, denoted as Anabaena CPB4337, showed a high constitutive luminescence with no need to add exogenous aldehyde. The tested factors were pH, EDTA (as organic ligand), and anions PO(4)(3-), CO(3)(2-), and Cl(-). Chemical modeling and correlation analyses were used to predict metal speciation and link it with toxicity. In general, metal toxicity significantly correlated to the predicted metal free-ion concentration, although Zn-EDTA complexes and certain Hg chloro-complexes could also exhibit some toxicity to cyanobacteria. An interesting feature of metal toxicity to strain Anabaena CPB4337 was that low amounts of PO(4)(3-) and CO(3)(2-) increased metal toxicity; this effect could not be related to significant changes in metal speciation and could be attributed to a modulating effect of these anions on metal/uptake toxicity. The combination of toxicity studies that take into account a range of factors that might modulate metal toxicity with chemical modeling to predict changes in metal speciation might be useful for interpreting complex toxicity data. Finally, this cyanobacterial bioreporter, due to its ecological relevance as a primary producer, could be used as a tool for toxicity assessment in freshwater environments.
Rodea-Palomares
Ismael
I
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
González-García
Coral
C
Leganés
Francisco
F
Fernández-Piñas
Francisca
F
D017033:Anabaena / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D000838:Anions / Q000494:pharmacology; D001682:Biological Availability; D002254:Carbonates / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D049453:Luciferases, Bacterial / Q000235:genetics; D008163:Luminescent Measurements; D019216:Metals, Heavy / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D008956:Models, Chemical; D010710:Phosphates / Q000494:pharmacology; D020637:Photorhabdus / Q000201:enzymology / Q000235:genetics; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*
D000838:Anions; D002254:Carbonates; D002712:Chlorides; D019216:Metals, Heavy; D010710:Phosphates; D014874:Water Pollutants, Chemical; D004492:Edetic Acid; D049453:Luciferases, Bacterial