[AZF microdeletions are not related with recurrent spontaneous abortion].
OBJECTIVE
To study the relationship between azoospermia factor (AZF) microdeletions of the Y-chromosome and recurrent spontaneous abortion.


METHODS
We collected 26 chorionic villus samples from abortive male embryos and 51 blood samples from the husbands whose wives had recurrent spontaneous abortion, extracted genomic DNA from the samples and detected 12 STSs in the AZFa, AZFb and AZFc regions of Yq11.2 by amplification multiplex PCR.


RESULTS
AZF microdeletions were found neither in the chorionic villus samples nor in the blood samples.


CONCLUSIONS
There is no relationship between AZF microdeletions and recurrent spontaneous abortion.
Lu
Hong-Yong
HY
PLA Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. lu_lhy@163.com
Cui
Ying-Xia
YX
Xia
Xin-Yi
XY
Shi
Yi-Chao
YC
Yang
Bin
B
Shao
Yong
Y
Ge
Yi-Feng
YF
Yao
Bing
B
Li
Xiao-Jun
XJ
Huang
Yu-Feng
YF
D000026:Abortion, Habitual / Q000235:genetics*; D000022:Abortion, Spontaneous / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D005260:Female; D056426:Genetic Loci; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D029589:Seminal Plasma Proteins / Q000235:genetics*
D029589:Seminal Plasma Proteins