title: Stretching single talin rod molecules activates vinculin binding.
abstract: The molecular mechanism by which a mechanical stimulus is translated into a chemical response in biological systems is still unclear. We show that mechanical stretching of single cytoplasmic proteins can activate binding of other molecules. We used magnetic tweezers, total internal reflection fluorescence, and atomic force microscopy to investigate the effect of force on the interaction between talin, a protein that links liganded membrane integrins to the cytoskeleton, and vinculin, a focal adhesion protein that is activated by talin binding, leading to reorganization of the cytoskeleton. Application of physiologically relevant forces caused stretching of single talin rods that exposed cryptic binding sites for vinculin. Thus in the talin-vinculin system, molecular mechanotransduction can occur by protein binding after exposure of buried binding sites in the talin-vinculin system. Such protein stretching may be a more general mechanism for force transduction.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
authors.forename: Armando
authors.initials: A
authors.lastname: del Rio
authors.forename: Raul
authors.initials: R
authors.lastname: Perez-Jimenez
authors.forename: Ruchuan
authors.initials: R
authors.lastname: Liu
authors.forename: Pere
authors.initials: P
authors.lastname: Roca-Cusachs
authors.forename: Julio M
authors.initials: JM
authors.lastname: Fernandez
authors.forename: Michael P
authors.initials: MP
authors.lastname: Sheetz
mesh_terms: D000818:Animals; D001665:Binding Sites; D055592:Biophysical Phenomena; D002645:Chickens; D040542:Mechanotransduction, Cellular; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D038761:Photobleaching; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016608:Talin / Q000737:chemistry* / Q000378:metabolism*; D016596:Vinculin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D016608:Talin; D016596:Vinculin