Expression patterns of the Egr1 and Egr3 genes during medaka embryonic development.
Egr1 and Egr3 are zinc finger-type transcription factors and known as synaptic activity-inducible immediate-early genes. Egr1 also plays important roles in many aspects of vertebrate development. Egr3 is known as the gene that is related to biological rhythm and muscular development, but its behavior in the central nervous system during development is not clear. We cloned the cDNA of the egr1 and egr3 orthologs in medaka, and examined their expression patterns during embryonic development using whole-mount in situ hybridization. Medaka egr3 was the first cloned egr3 gene in fish. The expression of egr1 mRNA was first detected at 1day post-fertilization (dpf). It was expressed in the whole embryonic body. At 3dpf, the egr1 mRNA was strongly expressed in the telencephalon, diencephalon, hypothalamus, optic tectum, dorsal medulla oblongata, retina, heart, pharynx, and pectoral fin. The expression of egr3 mRNA was first detected at 3dpf. It was expressed in the telencephalon, hypothalamus, optic tectum, and pharynx. By sectioning the whole-mount specimens, expression of both the egr1 and egr3 mRNAs were observed in the telencephalon, hypothalamus, and optic tectum. However, the positions at which the genes were expressed were different.
Deguchi
Tomonori
T
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo 661-0974, Japan. tomonori-deguchi@aist.go.jp
Fujimori
Kazuhiro E
KE
Kawasaki
Takashi
T
Xianghai
Liao
L
Yuba
Shunsuke
S
D000595:Amino Acid Sequence; D000818:Animals; D051766:Early Growth Response Protein 1 / Q000145:classification / Q000235:genetics*; D051776:Early Growth Response Protein 3 / Q000145:classification / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D007031:Hypothalamus / Q000196:embryology / Q000378:metabolism; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009899:Optic Lobe, Nonmammalian / Q000196:embryology / Q000378:metabolism; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013687:Telencephalon / Q000196:embryology / Q000378:metabolism; D013997:Time Factors
D051766:Early Growth Response Protein 1; D029941:Fish Proteins; D012333:RNA, Messenger; D051776:Early Growth Response Protein 3