Engineering a function into a glycosyltransferase.
As glycosyltransferases found in nature often show distinct substrate specificity, glycosyltransferase engineering is an important research field. In this work, we were able to introduce an activity into a glycosyltransferase involved in natural product (landomycin E) biosynthesis. This was achieved by recognizing hot spot amino acids in glycosyltransferases which are strongly involved in determining substrate specificity.
Krauth
Christine
C
Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Freiburg, Germany.
Fedoryshyn
Marta
M
Schleberger
Christian
C
Luzhetskyy
Andriy
A
Bechthold
Andreas
A
D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015202:Protein Engineering; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D000617:Aminoglycosides; C113593:landomycin E; D016695:Glycosyltransferases