title: The orexigenic activity of the hypothalamic neuropeptide 26RFa is mediated by the neuropeptide Y and proopiomelanocortin neurons of the arcuate nucleus.
abstract: 26RFa is a hypothalamic RFamide neuropeptide that was identified as the endogenous ligand of the orphan G protein-coupled receptor, GPR103, and that stimulates appetite in mice. Up until now, the mechanism of action of 26RFa in the hypothalamic control of food intake remains unknown. The high density of GPR103 in the arcuate nucleus (Arc) prompted us to investigate, in the present study, the effects of 26RFa on the rat neuropeptide Y (NPY)/proopiomelanocortin (POMC) system. Intracerebroventricular injection of 26RFa stimulated NPY expression and release in the basal hypothalamus, whereas it decreased POMC expression and alpha-MSH release, and these effects were associated with an increase in food intake. A double in situ hybridization procedure indicated that the 26RFa receptor is present in NPY neurons of the Arc, but not in POMC neurons. Central administration of NPY Y1 and Y5 receptor antagonists abolished the inhibitory effects of 26RFa on POMC expression and alpha-MSH release, and reversed 26RFa-induced food consumption. Finally, 26RFa antagonized the effects of leptin on NPY expression and release, POMC expression and alpha-MSH release, and food intake. Altogether, the present data demonstrate for the first time that 26RFa exerts its orexigenic activity by stimulating the release of NPY in the Arc, which in turn inhibits POMC neurons by activating the Y1 and Y5 receptors. It is also suggested that the balance 26RFa/leptin is an important parameter in the maintenance of energy homeostasis.
authors.affiliation: Equipe Associée à l'université 4310/Institut National de la Santé et de la Recherche Médicale Unité 413, Neuronal and Neuroendocrine Differentiation and Communication, European Institute for Peptide Research, University of Rouen, F76031 Rouen, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Lectez
authors.forename: Lydie
authors.initials: L
authors.lastname: Jeandel
authors.forename: Fatima-Zohra
authors.initials: FZ
authors.lastname: El-Yamani
authors.forename: Sébastien
authors.initials: S
authors.lastname: Arthaud
authors.forename: David
authors.initials: D
authors.lastname: Alexandre
authors.forename: Aurélie
authors.initials: A
authors.lastname: Mardargent
authors.forename: Sylvie
authors.initials: S
authors.lastname: Jégou
authors.forename: Lourdes
authors.initials: L
authors.lastname: Mounien
authors.forename: Patrice
authors.initials: P
authors.lastname: Bizet
authors.forename: Rabia
authors.initials: R
authors.lastname: Magoul
authors.forename: Youssef
authors.initials: Y
authors.lastname: Anouar
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chartrel
mesh_terms: D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects* / Q000235:genetics; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004435:Eating / Q000187:drug effects / Q000235:genetics; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D007028:Hypothalamic Hormones / Q000008:administration & dosage / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007276:Injections, Intraventricular; D020738:Leptin / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009479:Neuropeptides / Q000008:administration & dosage / Q000494:pharmacology*; D011333:Pro-Opiomelanocortin / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D000521:alpha-MSH / Q000378:metabolism
chemical_list: D007028:Hypothalamic Hormones; D020738:Leptin; D009478:Neuropeptide Y; D009479:Neuropeptides; C480638:prepro-26RFa protein, human; D000521:alpha-MSH; D011333:Pro-Opiomelanocortin