Microseparation of membrane proteins.
Membrane proteins are generally of natural low abundance and insoluble to aqueous solutions. Thus, investigation of membrane proteins is relatively hampered since efficient separation of membrane proteins are rather challenging. Microseparation approaches certainly play an essential role in fulfilling such demanding investigations due to their significant advantages of high throughput, reduced separation time, and low sample consumption. In this review, recent developments of microseparation are documented with aspects of three key separation methods, including CE, micro-LC and microchip. Preparation of membrane proteins prior to separation is also discussed, including solubilization and preconcentration. Certainly, more applications of microseparation approaches in membrane protein separations can be expected in the near future.
Zhang
Zhaowei
Z
Division of Biomedical Photonics at Wuhan National Laboratory for Optoelectronics, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Feng
Xiaojun
X
Du
Wei
W
Luo
Qingming
Q
Liu
Bi-Feng
BF
D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000302:isolation & purification*; D040081:Protein Array Analysis; D012995:Solubility
D008565:Membrane Proteins