[A simple method of separating outer hair cells in Guinea pig].
OBJECTIVE
In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.


METHODS
The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.


RESULTS
A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.


CONCLUSIONS
If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
Kang
Xixun
X
Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Kong
Weijia
W
Li
Wanrong
W
Zeng
Xiaorong
X
Yang
Yan
Y
Zhang
Song
S
Guo
Chankkai
C
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003051:Cochlea / Q000166:cytology*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology*