title: The association between RCAS1 expression in laryngeal and pharyngeal cancer and its healthy stroma with cancer relapse.
abstract: BACKGROUND
  The purpose of this study has been to establish the level of RCAS1 - a membrane protein expressed in various cancer cells and able to induce apoptosis of CTLs and NK cells in pharyngeal and laryngeal cancer and its clear surgical margin - with respect to clinicopathological features and to patient's follow up and evaluate its possible role in cancer relapse.
  
  
  METHODS
  A total of 122 tissue samples were obtained: 51 samples from laryngeal and pharyngeal squamous cell carcinoma, 51 samples from the clear surgical margins of these tumors, and 20 tissue samples derived from the healthy mucous membranes of the upper respiratory tract mucosa of patients without cancerous tumors. Patients were observed for a total of 4 years following surgical treatment. The level of RCAS1 expression was assessed by immunohistochemistry and Western blot.
  
  
  RESULTS
  RCAS1 was identified in all laryngeal and pharyngeal carcinomas and in almost all the clear surgical margin samples. The level of RCAS1 expression was significantly higher in the cancerous samples than in the clear surgical margins and was determined to be related to the grade of the cancer and the presence of lymph node metastases. In cases of cancer relapse, significantly higher levels of RCAS1 expression were observed in the clear surgical margins.
  
  
  CONCLUSIONS
  Selective cytotoxic immune cell suppression concomitant with tumor growth and associated with RCAS1 expression seems to be an important event connected with cancer relapse.
authors.affiliation: ENT Head and Neck Surgery Department, the Jagiellonian University, Krakow, Poland. mowicher@gmail.com
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dutsch-Wicherek
authors.forename: Romana
authors.initials: R
authors.lastname: Tomaszewska
authors.forename: Agata
authors.initials: A
authors.lastname: Lazar
authors.forename: Lukasz
authors.initials: L
authors.lastname: Wicherek
authors.forename: Jacek
authors.initials: J
authors.lastname: Skladzien
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D004848:Epithelium / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000276:immunology* / Q000473:pathology; D007830:Larynx / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D010610:Pharyngeal Neoplasms / Q000276:immunology* / Q000473:pathology; D010614:Pharynx / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; C120762:EBAG9 protein, human