Immobilized metal affinity chromatography in the presence of arginine.
Arginine hydrochloride (ArgHCl) is a versatile solvent additive, as it suppresses protein aggregation. ArgHCl has been used for protein refolding and to solubilize proteins from loose inclusion bodies. Immobilized metal affinity chromatography (IMAC) is one of the most commonly used technologies for purification of recombinant proteins. Here we have evaluated compatibility of ArgHCl with IMAC purification for his-tag proteins. ArgHCl clearly interfered with protein binding to Ni-columns. Nevertheless, such interference was greatly reduced at ArgHCl concentration below 200 mM, demonstrating that IMAC purification can be done even in the presence of ArgHCl.
Abe
Ryota
R
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 301 FBS-Bldg., 5-1-5 Kashiwanoha, Kashiwa 277-8562, Japan.
Kudou
Motonori
M
Tanaka
Yoshikazu
Y
Arakawa
Tsutomu
T
Tsumoto
Kouhei
K
D001120:Arginine / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D009532:Nickel / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000302:isolation & purification*
C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011994:Recombinant Proteins; D006639:Histidine; D009532:Nickel; D001120:Arginine