title: Redox-sensitive green fluorescent protein: probes for dynamic intracellular redox responses. A review.
abstract: The quantification of transient redox events within subcellular compartments, such as those involved in certain signal transduction pathways, requires specific probes with high spatial and temporal resolution. Redox-sensitive variants of the green fluorescent protein (roGFP) have recently been developed that allow the noninvasive monitoring ofintracellular thiol-disulfide equilibria. In this chapter, the biophysical properties of these probes are discussed, including recent efforts to enhance their response times. Several recent applications of roGFPs are highlighted, including roGFP expression within Arabidopsis to monitor redox status during root elongation, expression in neurons to measure oxidative stress during ischemia, and targeting of roGFPs to endosomal compartments demonstrating unexpectedly oxidizing potentials within these compartments. Possible future directions for the optimization of roGFPs or new classes of redox-sensitive fluorescent probes are also discussed.
authors.affiliation: Department of Biochemistry and Physical Sciences, BYU-Hawaii, Laie, HI, USA.
authors.forename: Mark B
authors.initials: MB
authors.lastname: Cannon
authors.forename: S James
authors.initials: SJ
authors.lastname: Remington
mesh_terms: D000818:Animals; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D015335:Molecular Probes; D049452:Green Fluorescent Proteins