title: Keratinocyte-melanocyte graft technique followed by PUVA therapy for stable vitiligo.
abstract: BACKGROUND
  Various surgical procedures for correcting stable vitiligo exist but these have their own limitations. Autologous, non-cultured, non-trypsinized, melanocyte plus keratinocyte grafting is a new and simple method of vitiligo surgery.
  
  
  OBJECTIVE
  The study aimed to evaluate efficacy of a new grafting technique in vitiligo patches.
  
  
  METHODS
  Eighteen vitiligo patches underwent this procedure. The upper layer of epidermis was removed by superficial dermabrasion using a dermabrader micromotor until the epidermis appeared wet and shiny. Then, antibiotic ointment was applied and dermabrasion was continued up to the whitish area of the upper dermis. The paste-like material (ointment with entangled epidermal particles) was collected and spread over the dermabraded recipient site.
  
  
  RESULTS
  Pigmentation usually started at 4-6 weeks. Complete uniform pigmentation took 16-20 weeks.
  
  
  CONCLUSIONS
  For smaller vitiligo patches this method gives cosmetically acceptable results. It is easy to perform and does not require specific laboratory setup.
authors.affiliation: Department of Dermatology, Venereology and Leprosy, Dr. S. N. Medical College, Jodhpur (Rajasthan), India. drdilipkachhawa@hotmail.com
authors.forename: Dilip
authors.initials: D
authors.lastname: Kachhawa
authors.forename: Gyaneshwar
authors.initials: G
authors.lastname: Kalla
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D003869:Dermabrasion / Q000379:methods; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008544:Melanocytes / Q000637:transplantation*; D011701:PUVA Therapy / Q000379:methods; D016038:Skin Transplantation / Q000379:methods*; D014820:Vitiligo / Q000188:drug therapy / Q000473:pathology / Q000601:surgery*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents