In vitro selection of hemin-binding catalytic RNA.
Catalytic RNAs with peroxidase activity were obtained by the in vitro selection of RNA aptamer-binding hemin. One of the RNA aptamers selected showed binding affinity to hemin with a dissociation constant of 0.8muM and exhibited high peroxidase activity by forming a complex with hemin. The catalytic efficiency of the RNA-hemin complex was 10-fold higher than that of hemin alone.
Liu
Mingzhe
M
Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Kagahara
Takuma
T
Abe
Hiroshi
H
Ito
Yoshihiro
Y
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D006427:Hemin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009195:Peroxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*
D016337:RNA, Catalytic; D006427:Hemin; D009195:Peroxidase