title: [Influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients].
abstract: OBJECTIVE
  To study the influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients.
  
  
  METHODS
  The brucine liposomes were separated by Sephadex G-50, and the influence of various factors on the entrapment efficiencies were investigated.
  
  
  RESULTS
  The entrapment efficiency was enhanced by increased ammonium sulfate concentration, ethanol volume and PC concentration.
  
  
  CONCLUSIONS
  Burcine liposomes prepared by ammonium sulfate transmembrance gradients can get a high entrapment efficiency, the main influencing factors were ammonium sulfate concentration, ethanol volume and PC concentration.
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
authors.forename: Yun
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry*; D019540:Area Under Curve; D004337:Drug Carriers; D004355:Drug Stability; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D013331:Strychnine / Q000031:analogs & derivatives* / Q000737:chemistry; D031451:Strychnos / Q000737:chemistry*; D013678:Technology, Pharmaceutical; D013696:Temperature
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D010743:Phospholipids; D000431:Ethanol; C083806:brucine; D013331:Strychnine; D000645:Ammonium Sulfate