[Diagnostic significance of serum allergen detection in diagnosis of allergic rhinitis and bronchial asthma].
OBJECTIVE
To analyze the diagnostic significance of serum total IgE, specific IgE (SIgE), Phadiatop, and eosinophil cationic protein (ECP) in allergic rhinitis and bronchial asthma.


METHODS
The serum total IgE, SIgE , and Phadiatop were tested in 122 patients with allergic rhinitis. The ECP, SIgE, and Phadiatop were tested in 135 patients with bronchial asthma. Forty healthy persons were used as controls. The serum total IgE were tested by electrochemiluminescence. The serum Phadiatop, ECP, and SIgE were tested by fluorescent-enzyme linked immunosorbent assay.


RESULTS
The serum total IgE positive rate of the 122 allergic rhinitis patients was 37.7%, significantly higher than that of the healthy controls (2.5%, chi2 = 18.13, P < 0.01). The specificity of total IgE of the allergic rhinitis patients were 97.5%. The serum ECP positive rate of the bronchial asthma patients was 65.9%, significantly higher than that of the healthy controls (20.0%, chi2 = 26.34, P < 0.01). The sensitivity and specificity of the bronchial asthma patients were 65.9% and 80.0%. The Phadiatop positive rates of the allergic rhinitis and the bronchial asthma patients were 50.0% and 31.9% respectively, both significantly higher than that of the control (0, chi2 = 32.08, 16.89, both P < 0.01). Most kinds of SIgE were from the allergens, such as dust mite, dust, and Artemisia etc.


CONCLUSIONS
Increase of serum ECP level is an important indicator of airway inflammation activity and inflammation serious degree in the bronchial asthma patients. The increase of serum total IgE has an important diagnostic significance in allergic rhinitis. It is important to detect SIgE and Phadiatop in diagnosis and monitoring of allergic rhinitis and bronchial asthma.
Fan
Chun-hong
CH
Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, China.
Yang
Tie-sheng
TS
Tang
Su-mei
SM
Wang
Qin
Q
Zhang
Zheng
Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000032:analysis* / Q000097:blood; D001249:Asthma / Q000175:diagnosis*; D016022:Case-Control Studies; D047150:Eosinophil Cationic Protein / Q000032:analysis / Q000097:blood; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000485:Allergens; D007073:Immunoglobulin E; D047150:Eosinophil Cationic Protein