title: Candida albicans ABG1 gene is involved in endocytosis.
abstract: The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in endocytosis.
authors.affiliation: The Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.
authors.forename: Verónica
authors.initials: V
authors.lastname: Veses
authors.forename: Manuel
authors.initials: M
authors.lastname: Casanova
authors.forename: Amelia
authors.initials: A
authors.lastname: Murgui
authors.forename: Neil A R
authors.initials: NA
authors.lastname: Gow
authors.forename: José P
authors.initials: JP
authors.lastname: Martínez
mesh_terms: D019677:Artificial Gene Fusion; D002176:Candida albicans / Q000502:physiology*; D004705:Endocytosis; D005656:Fungal Proteins / Q000378:metabolism*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D025301:Hyphae / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins