The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice.
Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.
Li
Fei
F
Department of Pediatric Development and Behavioral Shanghai, Children's Medical Center, School of Medicine, Shanghai Jiao Tung University, Shanghai, China.
Wang
Lei
L
Jin
Xin-Ming
XM
Yan
Cong-Huai
CH
Jiang
Shan
S
Shen
Xiao-Ming
XM
D000818:Animals; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000628:therapy*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006632:Histamine / Q000032:analysis / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology* / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010047:Ovalbumin / Q000276:immunology; D010957:Plasmids; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D007070:Immunoglobulin A; D053773:Transforming Growth Factor beta1; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine; D007371:Interferon-gamma; D010047:Ovalbumin; D048271:Chitosan