[Informatics analysis of malate dehydrogenase from Taenia saginata asiatica].
Tools from bioinformatics websites such as NCBI, ExPaSy were used for the analysis. The malate dehydrogenase full-length gene from Taenia saginata asiatica was 1 212 bp in length, with a coding region of 30-1 028 bp and coding 332 amino acids. It was a complete and full-length gene compared with the homologues in GenBank. The protein showed no transmembrane region, with stable physical-chemical characteristics. Three major linear epitopes located aa95-aa100, aa322-aa327 and aa117-aa122, with certain distance from each other on the surface of spatial structure of malate dehydrogenase (MDH). The last one was the linear epitope of Taenia. This cytosolic malate dehydrogenase gene is a potential antigen for diagnosis.
Huang
Jiang
J
Department of Parasitology, Guiyang Medical University, Guiyang 550004, China. mmm_hj@gmc.edu.cn
Hu
Xu-Chu
XC
Huang
Yan
Y
Yu
Xin-Bing
XB
Bao
Huai-En
HE
Lang
Shu-Yuan
SY
Liao
Xing-Jiang
XJ
D000818:Animals; D019295:Computational Biology; D018076:DNA, Complementary; D000939:Epitopes / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics*; D008291:Malate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D041202:Taenia saginata / Q000201:enzymology / Q000235:genetics* / Q000276:immunology
D018076:DNA, Complementary; D000939:Epitopes; D015801:Helminth Proteins; D008291:Malate Dehydrogenase