RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens.
CONCLUSIONS
We present RNAither, a package for the free statistical environment R which performs an analysis of high-throughput RNA interference (RNAi) knock-down experiments, generating lists of relevant genes and pathways out of raw experimental data. The library provides a quality assessment of the signal intensities, as well as a broad range of options for data normalization, different statistical tests for the identification of significant siRNAs, and a significance analysis of the biological processes involving corresponding genes. The results of the analysis are presented as a set of HTML pages. Additionally, all values and plots are available as either text files or pdf and png files.


BACKGROUND
http://bioconductor.org/
Rieber
Nora
N
Viroquant Research Group Modeling, University of Heidelberg, Bioquant BQ26, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany. RNAither@gmx.de
Knapp
Bettina
B
Eils
Roland
R
Kaderali
Lars
L
D019295:Computational Biology / Q000379:methods; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry; D012984:Software
D034741:RNA, Small Interfering