Enzymatic enrichment of egg-yolk phosphatidylcholine with alpha-linolenic acid.
alpha-Linolenic acid (ALA) was incorporated at 28% into the sn-1 position of egg-yolk phospatidylcholine using Novozyme 435 in one-step transesterification process. Using phospholipase A(2) in a two-step process gave 25% incorporation of ALA into the sn-2 position.
Chojnacka
A
A
Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25, Wroclaw, 50-375, Poland. jeanna@wp.pl
Gładkowski
W
W
Kiełbowicz
G
G
Wawrzeńczyk
C
C
D004530:Egg Yolk / Q000737:chemistry*; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008049:Lipase / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000302:isolation & purification* / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D010713:Phosphatidylcholines; D017962:alpha-Linolenic Acid; C093667:Novozyme 435; D008049:Lipase