Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples.
Fast and sensitive detection of microcystin-LR (MC-LR) was conducted with a portable trace organic pollutant analyzer (TOPA) based on the principle of immunoassay and total internal reflection fluorescence. The reusable fiber optic probe surface was produced by covalently immobilizing a MC-LR-ovalbumin (MC-LR-OVA) conjugate onto a self-assembled thiol-silane monolayer of fiber optic probe through a heterobifunctional reagent. It has been established that the MC-LR-OVA immobilized fiber optic probe is highly resistive to non-specific binding of proteins, while bound anti-MC-LR antibody can easily be eluted from the immunosurface with high recoveries (more than 150 assay cycles) in the way of pepsin solution, without any damage to the surface-immobilized MC-LR derivatives. One assay cycle including surface regeneration was less than 20 min. For the standard curve, the limit of detection (LOD) of 0.03 microg/L and the quantitative detection range of 0.1-10.1 microg/L was obtained when the concentration of antibody labeled by Cy5.5 was 0.28 microg/mL. Cross-reactivity against a few compounds structurally similar to MC-LR was little. The developed immunosensor method was applied to the monitoring of MC-LR in various types of water. The recovery of MC-LR added to water samples at different concentrations ranged from 80 to 110% with relative standard deviation (R.S.D.) values less than 5%. The immunoassay performance of the TOPA was validated with respect to conventional high-performance liquid chromatography, and the correlation between methods was in good agreement (r(2)=0.9978). The TOPA is a portable, easy-to-use, and robust immunoassay system and commercially obtained from the company JQ-environ Co. Ltd. (China).
Long
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Environmental Simulate and Pollution Control State Key Joint Laboratory, Dept. of Environment Science and Engineering, Tsinghua University, Beijing 100084, China.
He
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Zhu
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AN
Shi
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HC
D015374:Biosensing Techniques / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D008904:Miniaturization; D055096:Optical Devices; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; D014867:Water; C057862:cyanoginosin LR