title: A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.
abstract: A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: Juan A
authors.initials: JA
authors.lastname: González-Vera
authors.forename: Elvedin
authors.initials: E
authors.lastname: Luković
authors.forename: Barbara
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D064127:Aurora Kinases; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D005456:Fluorescent Dyes; D019151:Peptide Library; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005456:Fluorescent Dyes; D019151:Peptide Library; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases