title: Antimutagenic activity and radical scavenging activity of water infusions and phenolics from ligustrum plants leaves.
abstract: Water infusions of Ligustrum delavayanum and Ligustrum vulgare leaves and eight phenolics isolated therefrom have been assayed in vitro on ofloxacin-induced genotoxicity in the unicellular flagellate Euglena gracilis. The tested compounds luteolin, quercetin, luteolin-7-glucoside, luteolin-7-rutinoside, quercetin-3-rutinoside, apigenin-7-rutinoside, tyrosol and esculetin inhibited the mutagenic activity of ofloxacin (43 microM) in E. gracilis. Water infusions from leaves of L. delavayanum and L. vulgare showed higher antimutagenic effect (p(t) < 0.001). The activity of these samples against ofloxacin (86 microM)-induced genotoxicity was lower, but statistically significant (p(t) < 0.05), excluding the water infusion of L. delavayanum leaves (p(t) < 0.01). Efficacy of quercetin, luteolin-7-rutinoside, apigenin-7-rutinoside was insignificant. The antimutagenic effect of most phenolics we studied could be clearly ascribed to their DPPH scavenging activity, substitution patterns and lipophilicity.
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic. nagy@fpharm.uniba.sk
authors.forename: Milan
authors.initials: M
authors.lastname: Nagy
authors.forename: Lívia
authors.initials: L
authors.lastname: Krizková
authors.forename: Pavel
authors.initials: P
authors.lastname: Mucaji
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kontseková
authors.forename: Frantisek
authors.initials: F
authors.lastname: Sersen
authors.forename: Juraj
authors.initials: J
authors.lastname: Krajcovic
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000737:chemistry* / Q000378:metabolism; D001713:Biphenyl Compounds / Q000737:chemistry; D005056:Euglena gracilis / Q000187:drug effects / Q000235:genetics; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism; D006834:Hydrazines / Q000737:chemistry; D031659:Ligustrum / Q000737:chemistry*; D015242:Ofloxacin / Q000378:metabolism / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D014867:Water
chemical_list: D000900:Anti-Bacterial Agents; D016587:Antimutagenic Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D014867:Water; D015242:Ofloxacin; C004931:1,1-diphenyl-2-picrylhydrazyl