title: Response of rat osteoblasts to polycaprolactone/chitosan blend porous scaffolds.
abstract: Novel porous polycaprolactone/chitosan blend scaffolds were fabricated via a particle-leaching technique using hexafluoro-2-propanol as a shared solvent and salt particles as porogen. By selecting optimized processing conditions, numerous blend scaffolds were already built with well-controlled porous structures and approximately similar pore parameters. The interactions between these scaffolds and the rat osteoblasts were further investigated by measuring the adhesion, proliferation, and morphologies of cells as well as the corresponding alkaline phosphatase activity during various culture periods in vitro. Data collected from different measurements reflected that by incorporating an appropriate amount of chitosan component, the resultant blend scaffolds could improve the adhesion and growth of osteoblasts and exert a significant impact on the osteoblastic phenotype.
authors.affiliation: Department of Nuclear Medicine, Xiamen First Hospital, Fujian Medical University, Xiamen 301003, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
authors.forename: Siqin
authors.initials: S
authors.lastname: Dalai
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan; D000469:Alkaline Phosphatase