title: Influence of bone marrow graft B lymphocyte subsets on outcome after HLA-identical sibling transplants.
abstract: The potential role of the infused B cell subset after Hematopoietic Stem Cell Transplantation has not been yet studied. The present study analyzed the impact of B cells on transplant outcome in 254 patients who received a bone marrow graft from a human leucocyte antigen-identical sibling donor. The influence of B lineage-specific hematopoietic progenitor cells (CD34(+) CD19(+)) and B cells (immature and mature B cells, CD34(-) CD19(+)) was also analyzed. All included patients received a myeloablative regimen. The cumulative incidence function of acute graft-versus-host (GvHD) grade II to IV was 48% and was inversely associated with the number of CD34(+) CD19(+). There were no statistically significant associations between B cell subsets and chronic GvHD or survival. The CD34(+) CD19(+) B cell subset remained significantly associated with acute GvHD in multivariate analysis (Relative risk = 0.32, 95% confidence interval: 0.11-0.92, P = 0.035). In conclusion, a higher B lineage-specific hematopoietic progenitor cells (CD34(+) CD19(+)) cell dose is associated with a significant decrease incidence of acute GvHD.
authors.affiliation: Service d'Hématologie Greffe, Hôpital Saint Louis, Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Michonneau
authors.forename: Régis
authors.initials: R
authors.lastname: Peffault de Latour
authors.forename: Raphaël
authors.initials: R
authors.lastname: Porcher
authors.forename: Marie
authors.initials: M
authors.lastname: Robin
authors.forename: Marc
authors.initials: M
authors.lastname: Benbunan
authors.forename: Vanderson
authors.initials: V
authors.lastname: Rocha
authors.forename: Patricia
authors.initials: P
authors.lastname: Ribaud
authors.forename: Christele
authors.initials: C
authors.lastname: Ferry
authors.forename: Agnes
authors.initials: A
authors.lastname: Devergie
authors.forename: Valérie
authors.initials: V
authors.lastname: Vanneaux
authors.forename: Eliane
authors.initials: E
authors.lastname: Gluckman
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Marolleau
authors.forename: Gérard
authors.initials: G
authors.lastname: Socié
authors.forename: Jérôme
authors.initials: J
authors.lastname: Larghero
mesh_terms: D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000276:immunology*; D018952:Antigens, CD34 / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001855:Bone Marrow Diseases / Q000601:surgery; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000276:immunology; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D016016:Proportional Hazards Models; D012306:Risk; D035781:Siblings; D013234:Stem Cells / Q000276:immunology; D015996:Survival Rate; D014185:Transplantation, Isogeneic
chemical_list: D018941:Antigens, CD19; D018952:Antigens, CD34