title: Inhibition of hemolysate-induced iNOS and COX-2 expression by genistein through suppression of NF-small ka, CyrillicB activation in primary astrocytes.
abstract: Genistein is a major isoflavone compound from soybean. We investigated the anti-inflammatory properties of genistein in primary astrocytes treated with hemolysate. The results indicated that genistein inhibited the expression of hemolysate induced iNOS and COX-2 mRNA on astrocytes. Furthermore, this compound inhibited the level of hemolysate-stimulated nuclear factor-kappa B (NF-small ka, CyrillicB). Therefore, we suggested that the effect of genistein-mediated inhibition of the expression hemolysate-induced iNOS and COX-2 genes is due to under the suppression of NF-small ka, CyrillicB activation in the transcriptional level.
authors.affiliation: Department of Neurosurgery, Jinling Hospital, and The State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 305 East Zhongshan Road, Nanjing 210002, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Shi
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Zhang
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Wang
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Hang
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Chen
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Yin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D016328:NF-kappa B; D012333:RNA, Messenger; D019833:Genistein; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051546:Cyclooxygenase 2