Improving effect of pretreatment with yiqifumai on LPS-induced microcirculatory disturbance in rat mesentery.
Yiqifumai is a traditional Chinese medicine compound preparation used for the treatment of various vascular diseases in China. However, little is known regarding its role in microcirculation. The present study investigated the effect of pretreatment of yiqifumai on rat mesentery microcirculatory disturbance induced by LPS. Male Wistar rats were continuously infused with LPS (5 mg kg(-1) h(-1)). The parameters evaluated included diameter of and red blood cell velocity in venules, leukocyte adhesion to venular wall, dihydrorhodamine 123 (DHR) fluorescence in the venular walls, fluorescein isothiocyanate-albumin leakage, and mast cell degranulation, which were observed by an inverted intravital microscope. CD11b/CD18 expression on neutrophils was examined using flow cytometry. In some rats, yiqifumai (5, 30, or 80 mg kg(-1)) was given in one shot 10 min before LPS infusion. After infusion of LPS, the number of leukocytes adherent to venular wall, the intensity of DHR fluorescence in the venular walls, albumin leakage from venules, and degranulated mast cells were significantly increased, whereas the red blood cell velocity in venule was decreased. Pretreatment with high-dose yiqifumai (80 mg kg(-1)) significantly reduced the number of adherent leukocytes, the intensity of DHR fluorescence, degranulation of mast cell, albumin leakage, and the expression of CD11b/CD18, whereas the yiqifumai of medium dose (30 mg kg(-1)) only inhibited leukocyte adhesion to the venular wall. The results suggested that pretreatment with yiqifumai attenuated microcirculatory disturbance induced by LPS. This effect may be associated with yiqifumai's inhibition effect on reactive oxygen species production, leukocyte adhesion, and mast cell degranulation.
Yuan
Qing
Q
Department of Intensive Care Unit, Beijing Friendship Hospital Affiliated to Capital University of Medical Science, Beijing, China.
Liu
Yu-Ying
YY
Sun
Kai
K
Chen
Chun-Hua
CH
Zhou
Chang-Man
CM
Wang
Chuan-She
CS
Li
Ang
A
Zhang
Shu-Wen
SW
Ye
Zheng-Liang
ZL
Fan
Jing-Yu
JY
Han
Jing-Yan
JY
D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008643:Mesentery / Q000098:blood supply* / Q000187:drug effects*; D008833:Microcirculation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D039481:CD11b Antigen; D018821:CD18 Antigens; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides