[Loss of heterozygosity on chromosome 10 is associated with bone metastasis of human prostate cancer].
OBJECTIVE
To study the cytogenetic mechanism of bone metastasis of human prostate cancer (PCa).


METHODS
We analyzed chromosome variation by comparative genomic hybridization in 18 patients with prostate cancer to determine the chromosome variants associated with bone metastasis, and focused on 7 microsatellite sites on chromosome 10 for the detection of the loss of heterozygosity (LOH) by PCR-based microsatellite polymorphism analysis.


RESULTS
In the 11 samples with bone metastasis, the variation rate of chromosome 10 was 90.9% (10/11), significantly higher than that of the others (P < 0.01). A much higher LOH frequency was observed at the 7 microsatellite loci on chromosome 10 and the highest located in 10q24. 2-q25.3 (D10S1693-D10S587) in the PCa patients with bone metastasis.


CONCLUSIONS
There is a high-frequency LOH region in 10q24. 2-q25.3 (D10S1693-D10S587) on chromosome 10 in PCa patients with bone metastasis, which may be potentially involved in PCa progression and specific bone metastasis.
Hou
Zhu
Z
Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China. houzhuanzhen@sohu.com
Luo
Yong
Y
Jiang
Yong-guang
YG
Li
Ming-chuan
MC
Lin
Yun-hua
YH
Zhang
Yu-ping
YP
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001859:Bone Neoplasms / Q000235:genetics* / Q000556:secondary*; D002879:Chromosomes, Human, Pair 10; D055028:Comparative Genomic Hybridization; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*