Early detection of neurodegeneration in brain ischemia by manganese-enhanced MRI.
This study aims to employ in vivo manganese-enhanced MRI (MEMRI) to detect neurodegenerative changes in two models of brain ischemia, photothrombotic cortical injury (PCI) and transient middle cerebral artery occlusion (MCAO) in rodents. After systemic Mn(2+) injection to both ischemic models, a close pattern of T1-weighted hyperintensity was observed throughout different brain regions in comparison to the distribution of GFAP, MnSOD and GS immunoreactivities, whereby conventional MRI could hardly detect such. In addition, the infarct volumes in the posterior parts of the brain had significantly reduced after Mn(2+) injection to the MCAO model. It is suggested that exogenous Mn(2+) injection may provide enhanced MEMRI detection of oxidative stress and gliosis early after brain ischemia. Manganese may also mediate infarctions at remote brain regions in transient focal cerebral ischemia before delayed secondary damage takes place.
Chan
Kevin C
KC
Laboratory of Biomedical Imaging and Signal Processing and the Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam, China. kevin_ccw@hkuusa.hku.hk
Cai
Ke-xia
KX
Su
Huan-xing
HX
Hung
Victor K
VK
Cheung
Matthew M
MM
Chiu
Chi-tat
CT
Guo
Hua
H
Jian
Yang
Y
Chung
Sookja K
SK
Wu
Wu-tian
WT
Wu
Ed X
EX
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000473:pathology; D020520:Brain Infarction / Q000175:diagnosis / Q000473:pathology; D002545:Brain Ischemia / Q000175:diagnosis / Q000473:pathology*; D003627:Data Interpretation, Statistical; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015233:Models, Statistical; D019636:Neurodegenerative Diseases / Q000175:diagnosis / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008345:Manganese