Human sperm centrin levels & outcome of intracytoplasmic sperm injection (ICSI)--a pilot study.
OBJECTIVE
The objective of the present study was to compare the levels of sperm centrin a centrosomal protein that influences cell migration, in normal fertile donors and in oligoasthenozoospermic males (count 5 million/ml and motility <40%, grade c+d) undergoing intracytoplasmic sperm injection (ICSI) and to correlate with the outcome of ICSI.


METHODS
The prospective study carried out at Inkus IVF Centre, Mumbai, India, during (January-December 2003). It included 20 normal fertile donor males (group I) and 20 oligoasthnozoospermic (OA) males (group II). Group II was further divided in II a and II b according to the centrin levels. Centrin levels were measured by using enzyme linked immunosorbent assay (ELISA) in both groups. All participants underwent an ICSI procedure and the levels of centrin and outcome of ICSI were correlated.


RESULTS
Centrin levels were significantly lower (P<0.001) in group II (0.39) as compared with group I (1.34). With centrin levels <0.45 optical density (OD) (group II a) the pregnancy rate was further reduced, with only 2 pregnancies (out of 14) both of which, ended in abortion. Cases in group II showed levels of centrin much lower than in the fertile group. Further lowered centrin levels were associated with lowered pregnancy rates in OA males, but statistically was not significant.


CONCLUSIONS
The study revealed that lower centrin levels in OA males resulted in lower pregnancy percentage in this group after ICSI. Disturbances in centrosomal protein could be one of the possible causes of ICSI failure.
Hinduja
Indira
I
Jasolok Hospital & Research Centre, Mumbai, India. inkus@bom7.vsnl.net.in
Zaveri
Kusum
K
Baliga
Nishitha
N
D002135:Calcium-Binding Proteins / Q000032:analysis / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D010865:Pilot Projects; D011247:Pregnancy; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000737:chemistry*
D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; C056608:caltractin