Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007-June 2008.
This study describes the prevalence of culture-positive adenovirus serotypes in culture-positive respiratory specimens sent to the Central Public Health Laboratory, Toronto, Ontario, Canada for the period September 2007-June 2008. Total nucleic acid was extracted from virus cultures using an automated extraction method followed by polymerase chain reaction and Sanger sequencing of the adenovirus hexon gene hypervariable region 7. 73% of specimens (n = 70) were from patients < or = 4 years of age. Of the 96 adenovirus isolates, the most common identified serotypes were serotype 3 (n = 44, 46%), serotype 2 (n = 25, 26%), serotype 1 (n = 17, 18%), and serotype 21 (n = 5, 5%). Adenovirus serotype 14 was not found in this study group. The leading serotype, Ad3, was identified throughout the duration of the study period. Molecular methods allow for the determination of circulating adenovirus serotypes and be used to document the spread of highly virulent adenoviral serotypes into a region.
Yeung
Rani
R
Ministry of Health and Long-Term Care, Public Health Laboratories Branch, Etobicoke, Ontario, Canada. rani.yeung@gmail.com
Eshaghi
AliReza
A
Lombos
Ernesto
E
Blair
Joanne
J
Mazzulli
Tony
T
Burton
Laura
L
Drews
Steven J
SJ
D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009864:Ontario / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012137:Respiratory System / Q000821:virology; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000821:virology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014776:Virus Cultivation
D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus