Caveolin-1 overexpression correlates with tumour progression markers in pancreatic ductal adenocarcinoma.
The assessment of caveolin-1 (Cav-1) as a marker of tumor aggressiveness in pancreatic ductal adenocarcinoma (PDAC). In this study, we examined the expression of Cav-1 in 34 human PDAC tissue samples and the associated peritumoral tissues by immunohistochemistry and western blot. Additionally, we correlated Cav-1 expression with other tissue (Ki-67, p53) and serum (CA 19-9) tumor markers. In the tumor-derived tissue, both tumor cells and blood vessels expressed Cav-1. In contrast, in peritumoral tissue, Cav-1 expression was confined mainly to blood vessels and was only occasionally expressed in ductal or parenchymal cells. Western blot analysis confirmed the overexpression of Cav-1 in pancreatic tumors compared with peritumoral tissue. Cav-1 expression in tumor tissues was correlated with both the Ki-67 LI (r = 0.95, P < 0.0001) and p53 expression (chi2 = 9.91, P < 0.005). Overexpression of Cav-1 was associated with tumor size, grade and stage and Cav-1 expression in tumors was correlated with an increased serum level of CA 19-9 (r = 0.795, P < 0.001). Based on the results of this study, the inclusion of Cav-1 in a putative panel of biomarkers predicting pancreatic cancer aggressiveness is warranted.
Tanase
Cristiana Pistol
CP
Victor Babes National Institute of Pathology, 99-101 Splaiul Independentei, Bucharest, 050096 Romania.
Dima
Simona
S
Mihai
Mihaela
M
Raducan
Elena
E
Nicolescu
Mihnea Ioan
MI
Albulescu
Lucian
L
Voiculescu
Bogdan
B
Dumitrascu
Traian
T
Cruceru
Linda Maria
LM
Leabu
Mircea
M
Popescu
Irinel
I
Hinescu
Mihail Eugen
ME
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D015153:Blotting, Western; D018395:CA-19-9 Antigen / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000097:blood; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D051242:Caveolin 1 / Q000378:metabolism*; D016009:Chi-Square Distribution; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010179:Pancreas / Q000737:chemistry / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D047368:Tumor Burden; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D002272:Carcinoembryonic Antigen; D051242:Caveolin 1; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53