Field-amplified sample injection for the determination of albumin and transferrin in human urines by MEKC.
An MEKC method combining field-amplified sample injection (FASI) has been developed for the analysis of albumin (Alb) and transferrin (TRF) in human urines. Various parameters affecting FASI preconcentration were optimized systematically. Under the optimum conditions, using electrokinetic sample injection for 90 s, the sensitivity was improved 77.6- and 45.3-fold for TRF and Alb, respectively, without loss of separation efficiency when compared with hydrodynamical sample injection for 5 s (0.5 psi). The detection limits of TRF and Alb were found to be 0.31 and 0.14 mg/L, respectively. The proposed method has been successfully applied to the determination of TRF and Alb in desalted urines from patients with kidney diseases, and the recoveries of TRF and Alb were 91.1-101.7%.
Wu
Yi-Wei
YW
College of Chemistry and Environmental Engineering, College of Life Sciences, Hubei Normal University, Hubei Province, Huangshi, P. R. China. chemwuyiwei@tom.com
Liu
Jun-Feng
JF
Xiao
Tang-Xin
TX
Han
De-Yan
DY
Zhang
Hai-Li
HL
Pan
Ji-Cheng
JC
D000097:Acetonitriles / Q000737:chemistry; D000418:Albumins / Q000302:isolation & purification*; D000419:Albuminuria; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014168:Transferrin / Q000302:isolation & purification / Q000652:urine*; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D000418:Albumins; D012997:Solvents; D014168:Transferrin; D014867:Water; D012965:Sodium Chloride; D014269:Trifluoroacetic Acid