title: Analysis of mRNA decay in Bacillus subtilis.
abstract: Studies of mRNA turnover in B. subtilis are less well known than in E. coli. Here we provide researchers who have an interest in gram-positive RNA processing with several protocols for RNA isolation, for 5'- and 3'-mapping of mRNAs and mRNA decay fragments, and we also include a comprehensive listing of B. subtilis mutants that are deficient in ribonucleases thought to be involved in mRNA decay.
authors.affiliation: Mount Sinai School of Medicine of New York University, Department of Pharmacology and Systems Therapeutics, New York, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Bechhofer
authors.forename: Irina A
authors.initials: IA
authors.lastname: Oussenko
authors.forename: Gintaras
authors.initials: G
authors.lastname: Deikus
authors.forename: Shiyi
authors.initials: S
authors.lastname: Yao
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mathy
authors.forename: Ciarán
authors.initials: C
authors.lastname: Condon
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D004591:Electrophoresis, Polyacrylamide Gel; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger