title: Evaluation of the osteogenic potential and vascularization of 3D poly(3)hydroxybutyrate scaffolds subcutaneously implanted in nude rats.
abstract: The aim of this study was to evaluate the osteogenic potential and the vascularization of embroidered, tissue engineered, and cell-seeded 3D poly(3)hydroxybutyrate (PHB) scaffolds in nude rats. Collagen I (coll I)- and collagen I/chondroitin sulfate (coll I/CS)-coated PHB scaffolds were seeded with human mesenchymal stem cells (hMSCs). Proliferation and differentiation were characterized by different biochemical assays in vitro. For animal experiments, the cells were cultivated on coll I- or coll I/CS-coated scaffolds and either expanded or osteogenically differentiated. Scaffolds were piled up to create a 3D scaffold pad and implanted subcutaneously into nude rats. In vitro hMSC showed proliferation and differentiation on PHB scaffolds. Alkaline phosphatase (ALP) and calcium increased in the differentiation medium and in the presence of coll I/CS. In vivo blood vessels were found in the scaffold-stack. Histological/immunohistological analyses of explanted scaffolds showed osteogenic markers such as osteopontin, osteonectin, and coll I around the PHB fibers. Coll I/CS-coated scaffolds with expanded hMSC showed higher values of ALP and calcium than the other combinations. Embroidered PHB scaffolds, coated with extracellular matrix components, provided an adequate environment and, therefore, a template for hMSC which could be differentiated in osteogenic direction.
authors.affiliation: Department of Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Strasse 74, 01307 Dresden, Germany. claudia.rentsch@uniklinikum-dresden.de
authors.forename: Claudia
authors.initials: C
authors.lastname: Rentsch
authors.forename: Barbe
authors.initials: B
authors.lastname: Rentsch
authors.forename: Annette
authors.initials: A
authors.lastname: Breier
authors.forename: Andre
authors.initials: A
authors.lastname: Hofmann
authors.forename: Suzanne
authors.initials: S
authors.lastname: Manthey
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Achim
authors.initials: A
authors.lastname: Biewener
authors.forename: Hans
authors.initials: H
authors.lastname: Zwipp
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024043:Collagen Type II / Q000494:pharmacology; D006801:Humans; D006885:Hydroxybutyrates / Q000494:pharmacology*; D007150:Immunohistochemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology*; D000091402:Prohibitins; D019919:Prosthesis Implantation; D051381:Rats; D011923:Rats, Nude; D040521:Subcutaneous Tissue / Q000000981:diagnostic imaging / Q000187:drug effects*; D013499:Surface Properties / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014057:Tomography, X-Ray Computed; D000094122:Polyhydroxybutyrates
chemical_list: D000469:Alkaline Phosphatase; D002118:Calcium; D024043:Collagen Type II; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; C000717328:PHB protein, human; C000717332:Phb protein, rat; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates