title: Quantitative matrix-assisted laser desorption ionization-fourier transform ion cyclotron resonance (MALDI-FT-ICR) peptide profiling and identification of multiple-sclerosis-related proteins.
abstract: We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).
authors.affiliation: Laboratories of Neuro-Oncology/Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam 3000 DR, The Netherlands.
authors.forename: Marcel P
authors.initials: MP
authors.lastname: Stoop
authors.forename: Lennard J
authors.initials: LJ
authors.lastname: Dekker
authors.forename: Mark K
authors.initials: MK
authors.lastname: Titulaer
authors.forename: Robert-Jan A N
authors.initials: RJ
authors.lastname: Lamers
authors.forename: Peter C
authors.initials: PC
authors.lastname: Burgers
authors.forename: Peter A E
authors.initials: PA
authors.lastname: Sillevis Smitt
authors.forename: Alain J
authors.initials: AJ
authors.lastname: van Gool
authors.forename: Theo M
authors.initials: TM
authors.lastname: Luider
authors.forename: Rogier Q
authors.initials: RQ
authors.lastname: Hintzen
mesh_terms: D000595:Amino Acid Sequence; D017357:Cyclotrons; D005583:Fourier Analysis; D006801:Humans; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D010455:Peptides / Q000134:cerebrospinal fluid*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D020543:Proteome