title: Integrated oligoaniline-cross-linked composites of Au nanoparticles/glucose oxidase electrodes: a generic paradigm for electrically contacted enzyme systems.
abstract: Sweet sensor: A bis-aniline-cross-linked Au nanoparticles (NPs)/glucose oxidase composite has been electropolymerized on a Au electrode (see picture). The integrated Au NPs/enzyme electrode provides an effective matrix for making electrical contact with the enzyme, and this yields a specific amperometric glucose biosensor.Electropolymerizable glucose oxidase (GOx) was synthesized by the tethering of thioaniline units to the protein. The electropolymerization of the thioaniline-modified GOx in the presence of thioaniline-functionalized Au nanoparticles (NPs) yielded a three-dimensional bis-aniline-cross-linked Au NPs/GOx network. The bis-aniline bridging units are redox active and mediate the electron transfer between the enzyme redox sites and the electrode, and the Au NPs provide the three-dimensional conductivity for transporting the electrons to the electrode. The covalently linked GOx displays an effective electrical contact with the electrode (turnover rate of ca. 500 s(-1)), which results in a sensitive and selective glucose-sensing electrode. The preparation of the electrode by means of electropolymerization, and the bioelectrocatalytic features of the electrode, suggest its feasibility as a miniaturized electrode with a sensing matrix and as an implantable glucose sensor in future applications.
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Omer
authors.initials: O
authors.lastname: Yehezkeli
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Yan
authors.forename: Ilina
authors.initials: I
authors.lastname: Baravik
authors.forename: Ran
authors.initials: R
authors.lastname: Tel-Vered
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles
chemical_list: D000814:Aniline Compounds; D006046:Gold; D005949:Glucose Oxidase