Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells.
Satellite cells are myogenic stem cells responsible for the postnatal regeneration of skeletal muscle. Here we report the successful in vitro induction of Pax7-positive satellite-like cells from mouse embryonic stem (mES) cells. Embryoid bodies were generated from mES cells and cultured on Matrigel-coated dishes with Dulbecco's modified Eagle medium containing fetal bovine serum and horse serum. Pax7-positive satellite-like cells were enriched by fluorescence-activated cell sorting using a novel anti-satellite cell antibody, SM/C-2.6. SM/C-2.6-positive cells efficiently differentiate into skeletal muscle fibers both in vitro and in vivo. Furthermore, the cells demonstrate satellite cell characteristics such as extensive self-renewal capacity in subsequent muscle injury model, long-term engraftment up to 24 wk, and the ability to be secondarily transplanted with remarkably high engraftment efficiency compared to myoblast transplantation. This is the first report of transplantable, functional satellite-like cells derived from mES cells and will provide a foundation for new therapies for degenerative muscle disorders.
Chang
Hsi
H
Department of Pediatrics, Kyoto University Graduate School of Medicine, 54 Syogoin Kawahara-cho Sakyo-ku, Kyoto 606-8507, Japan.
Yoshimoto
Momoko
M
Umeda
Katsutsugu
K
Iwasa
Toru
T
Mizuno
Yuta
Y
Fukada
So-ichiro
S
Yamamoto
Hiroshi
H
Motohashi
Norio
N
Miyagoe-Suzuki
Yuko
Y
Takeda
Shin'ichi
S
Heike
Toshio
T
Nakahata
Tatsutoshi
T
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000502:physiology*; D033581:Stem Cell Transplantation
D000906:Antibodies; D015415:Biomarkers; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse; D018995:Myosin Heavy Chains