An optimised protocol to prepare Phaeocystis globosa morphotypes for scanning electron microscopy observation.
A preparation protocol for scanning electron microscopy (SEM) was adapted and tested to the observation of Phaeocystis globosa morphotypes. For extra colonial cells, critical point drying (CPD) gave satisfactory results while for intra colonial cells, preservation with Lugol's iodine and/or glutaraldehyde followed by air-drying appeared as the most suitable method.
Guiselin
Natacha
N
LOG - UMR CNRS 8187 - Université du Littoral Côte d'Opale, Maison de la Recherche en Environnement Naturel, Wimereux, France. natacha.guiselin@univ-littoral.fr
Courcot
Lucie
L
Artigas
Luis Felipe
LF
Le Jéloux
Aude
A
Brylinski
Jean-Michel
JM
D000818:Animals; D016591:Histocytological Preparation Techniques / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D010839:Phytoplankton / Q000166:cytology* / Q000648:ultrastructure