Tocotrienol and alpha-tocopherol reduce corticosterone and noradrenalin levels in rats exposed to restraint stress.
This study investigates the effects of tocotrienol (TT) or alpha-tocopherol (TF) supplementation on corticosterone level, noradrenalin level and gastric lesions in rats exposed to restraint stress. Twenty-four male Sprague Dawley rats were randomly assigned into 4 equally sized groups; two control groups were given olive oil, while the treated group was supplemented with either tocotrienol of tocopherol orally at a dose of 60 mg/kg body weight. After 28 days of treatment, one control group, TT group and TF group were subjected to restraint stress, 2 hours daily for 4 consecutive days. After the last exposure to stress, plasma samples were taken to determine the corticosterone and noradrenalin levels, after which the rats were sacrificed. The stomach was excised for the evaluation of gastric lesions. Our findings showed that TT and TF were able to maintain the corticosterone level to the prestress values, while only TT was able to maintain the noradrenalin level in rats exposed to stress. Tocotrienol was found to be better in preventing formation of gastric lesions compared to TF. As a conclusion, the protective effect of vitamin E was related to the ability to inhibit stress induced elevation of corticosterone and noradrenalin levels.
Nur Azlina
M F
MF
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. nurazlina74@yahoo.com
Nafeeza
M I
MI
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003345:Corticosterone / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D016166:Free Radical Scavengers / Q000494:pharmacology; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009638:Norepinephrine / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013270:Stomach / Q000473:pathology; D013276:Stomach Ulcer / Q000097:blood / Q000209:etiology / Q000473:pathology; D013315:Stress, Psychological / Q000097:blood* / Q000150:complications; D024508:Tocotrienols / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D000975:Antioxidants; D016166:Free Radical Scavengers; D024508:Tocotrienols; D024502:alpha-Tocopherol; D003345:Corticosterone; D009638:Norepinephrine