Crystal structure of a novel Sm-like protein of putative cyanophage origin at 2.60 A resolution.
ECX21941 represents a very large family (over 600 members) of novel, ocean metagenome-specific proteins identified by clustering of the dataset from the Global Ocean Sampling expedition. The crystal structure of ECX21941 reveals unexpected similarity to Sm/LSm proteins, which are important RNA-binding proteins, despite no detectable sequence similarity. The ECX21941 protein assembles as a homopentamer in solution and in the crystal structure when expressed in Escherichia coli and represents the first pentameric structure for this Sm/LSm family of proteins, although the actual oligomeric form in vivo is currently not known. The genomic neighborhood analysis of ECX21941 and its homologs combined with sequence similarity searches suggest a cyanophage origin for this protein. The specific functions of members of this family are unknown, but our structure analysis of ECX21941 indicates nucleic acid-binding capabilities and suggests a role in RNA and/or DNA processing.
Das
Debanu
D
Joint Center for Structural Genomics, 2 Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Kozbial
Piotr
P
Axelrod
Herbert L
HL
Miller
Mitchell D
MD
McMullan
Daniel
D
Krishna
S Sri
SS
Abdubek
Polat
P
Acosta
Claire
C
Astakhova
Tamara
T
Burra
Prasad
P
Carlton
Dennis
D
Chen
Connie
C
Chiu
Hsiu-Ju
HJ
Clayton
Thomas
T
Deller
Marc C
MC
Duan
Lian
L
Elias
Ylva
Y
Elsliger
Marc-André
MA
Ernst
Dustin
D
Farr
Carol
C
Feuerhelm
Julie
J
Grzechnik
Anna
A
Grzechnik
Slawomir K
SK
Hale
Joanna
J
Han
Gye Won
GW
Jaroszewski
Lukasz
L
Jin
Kevin K
KK
Johnson
Hope A
HA
Klock
Heath E
HE
Knuth
Mark W
MW
Kumar
Abhinav
A
Marciano
David
D
Morse
Andrew T
AT
Murphy
Kevin D
KD
Nigoghossian
Edward
E
Nopakun
Amanda
A
Okach
Linda
L
Oommachen
Silvya
S
Paulsen
Jessica
J
Puckett
Christina
C
Reyes
Ron
R
Rife
Christopher L
CL
Sefcovic
Natasha
N
Sudek
Sebastian
S
Tien
Henry
H
Trame
Christine
C
Trout
Christina V
CV
van den Bedem
Henry
H
Weekes
Dana
D
White
Aprilfawn
A
Xu
Qingping
Q
Hodgson
Keith O
KO
Wooley
John
J
Deacon
Ashley M
AM
Godzik
Adam
A
Lesley
Scott A
SA
Wilson
Ian A
IA
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D030541:Databases, Genetic; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D011487:Protein Conformation; D055503:Protein Multimerization; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D016601:RNA-Binding Proteins