Effects of resveratrol on nucleotide degrading enzymes in streptozotocin-induced diabetic rats.
OBJECTIVE
Diabetes mellitus is associated with platelet alterations that may contribute to the development of cardiovascular complications. The present study investigates the effects of resveratrol (RSV), an important compound with cardioprotective activities, on NTPDase, ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP), 5'-nucleotidase and adenosine deaminase (ADA) activities in platelets from streptozotocin (STZ)-induced diabetic rats.


METHODS
The animals were divided into six groups (n=8): control/saline; control/RSV 10 mg/kg; control/RSV 20 mg/kg; diabetic/saline; diabetic/RSV 10 mg/kg; diabetic/RSV 20 mg/kg. RSV was administered during 30 days and after this period the blood was collected for enzymatic assay.


RESULTS
The results demonstrated that NTPDase, E-NPP and 5'-nucleotidase activities were significantly higher in the diabetic/saline group (P<0.05) compared to control/saline group. Treatment with RSV significantly increased NTPDase, 5'-nucleotidase and E-NPP activities in the diabetic/RSV10 and diabetic/RSV20 groups (P<0.05) compared to diabetic/saline group. When RSV was administered per se there was also an increase in the activities of these enzymes in the control/RSV10 and control/RSV20 groups (P<0.05) compared to control/saline group. ADA activity was significantly increased in the diabetic/saline group (P<0.05) compared to control/saline group. The treatment with RSV prevented this increase in the diabetic/RSV10 and diabetic/RSV20 groups. No significant differences in ADA activity were observed in the control/RSV10 and control/RSV20 compared to control/saline group.


CONCLUSIONS
The present findings demonstrate alterations in nucleotide hydrolysis in platelets of STZ-induced diabetic rats and treatment with RSV was able to modulate adenine nucleotide hydrolysis, which may be important in the control of the platelet coagulant status in diabetes.
Schmatz
Roberta
R
Programa de Pós Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. veramorsch@gmail.com
Schetinger
Maria Rosa Chitolina
MR
Spanevello
Roselia Maria
RM
Mazzanti
Cinthia Melazzo
CM
Stefanello
Naiara
N
Maldonado
Paula Acosta
PA
Gutierres
Jessié
J
Corrêa
Maísa de Carvalho
Mde C
Girotto
Eduardo
E
Moretto
Maria Beatriz
MB
Morsch
Vera Maria
VM
D015720:5'-Nucleotidase / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D013311:Streptozocin
D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D013311:Streptozocin; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D011755:Pyrophosphatases; D000077185:Resveratrol