title: On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS.
abstract: This chapter presents a general procedure for the on-chip detection and quantitation of low-molecular-weight recombinant proteins in transgenic plant crude extracts by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). A protocol is first described to detect the protein of interest in crude protein extracts of transgenic plant lines, by differential protein mapping against similar extracts from a control, nontransgenic line. A complementary protocol is then presented to generate a standard curve with the SELDI system, allowing the protein to be quantified in different transgenic lines. Overall, this procedure may be carried out within a few hours, without the need for prior purification or enrichment of the recombinant protein.
authors.affiliation: Département de phytologie, Université Laval, Québec, Canada.
authors.forename: Amine M
authors.initials: AM
authors.lastname: Badri
authors.forename: Karine
authors.initials: K
authors.lastname: Coenen
authors.forename: Louis-Philippe
authors.initials: LP
authors.lastname: Vaillancourt
authors.forename: Charles
authors.initials: C
authors.lastname: Goulet
authors.forename: Dominique
authors.initials: D
authors.lastname: Michaud
mesh_terms: D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010936:Plant Extracts; D011994:Recombinant Proteins