title: Secretory products from human adipocytes stimulate proinflammatory cytokine secretion from human endothelial cells.
abstract: Hyperplasia and hypertrophy of fat cells can be found in obesity and increased adiposity is associated with endothelial dysfunction as an early event of atherosclerosis. However, it is unclear whether human adipocytes directly influence endothelial protein secretion. To study the crosstalk between fat and endothelial cells, human umbilical venous endothelial cells (HUVECs) were cultured in infranatants (Adipo) of primary differentiated human adipocytes. Interestingly, significantly increased secretion of 23 cytokines and chemokines from HUVECs was detected in four independent experiments after Adipo stimulation by protein array analysis detecting a total of 174 different proteins. Among those, time-dependent Adipo-induced upregulation of cytokine secretion in HUVECs was confirmed by ELISA for interleukin (IL)-8, monokine induced by gamma interferon, macrophage inflammatory protein (MIP)-1beta, MIP-3alpha, monocyte chemoattractant protein-1, and IL-6. Factors besides adiponectin, leptin, resistin, and tumor necrosis factor alpha appear to mediate these stimulatory effects. Our findings suggest that endothelial cell secretion is significantly influenced towards a proinflammatory pattern by adipocyte-secreted factors.
authors.affiliation: Department of Internal Medicine III, University of Leipzig, Leipzig, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Sommer
authors.forename: Susan
authors.initials: S
authors.lastname: Kralisch
authors.forename: Verena
authors.initials: V
authors.lastname: Stangl
authors.forename: Angelika
authors.initials: A
authors.lastname: Vietzke
authors.forename: Uwe
authors.initials: U
authors.lastname: Köhler
authors.forename: Holger
authors.initials: H
authors.lastname: Stepan
authors.forename: Renaldo
authors.initials: R
authors.lastname: Faber
authors.forename: Andreas
authors.initials: A
authors.lastname: Schubert
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lössner
authors.forename: Matthias
authors.initials: M
authors.lastname: Bluher
authors.forename: Michael
authors.initials: M
authors.lastname: Stumvoll
authors.forename: Mathias
authors.initials: M
authors.lastname: Fasshauer
mesh_terms: D017667:Adipocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D018836:Inflammation Mediators; D019899:Paracrine Communication / Q000276:immunology*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D011506:Proteins