title: Glycoprofile of the different cell types present in the mucosa of the horse guttural pouches.
abstract: Histochemical characterization of the equine guttural pouches was performed using lectins combined with sialidase digestion and deglycosylation pre-treatments. The goblet cells contained O- and N-linked oligosaccharides with alpha-Fuc, GlcNAc moieties whereas beta-GalNAc, beta-Gal-(1-3)-GalNAc, beta-Gal-(1-4)-GlcNAc and alpha-Gal residues belonged only to O-linked glycoproteins. The acinar and ductal cells expressed alpha-Man/alpha-Glc in N-linked oligosaccharides, GlcNAc in both O- and N-glycoproteins and beta-GalNAc, beta-Gal-(1-3)-GalNAc, beta-Gal-(1-4)-GlcNAc and alpha-Gal residues included in O-linked glycoproteins. The Golgi area of the epithelial lining expressed alpha-Fuc in O-linked glycoproteins, internal GlcNAc in N-linked glycoproteins and large amounts of sialic acid residues linked to subterminal beta-GalNAc, Galbeta1,4GlcNAc and Galbeta1,3GalNAc. High amounts of sulpho-carbohydrates and of sialic acids (alpha2,3-6), linked to-alpha/beta-Gal and sialic acids (alpha2-6) linked to beta-GalNAc, were also demonstrated. Such diversity of the mucin saccharide residues may be implicated in the binding of macromolecules such as those of bacterial or viral etiology, thus playing a role in the organism's host-defense mechanism in the guttural pouches.
authors.affiliation: Department of Veterinary Science - Faculty of Veterinary Medicine, University of Camerino-UNICAM, via circonvallazione 93-95, 62024 Matelica, Italy. francesco.parillo@unicam.it
authors.forename: F
authors.initials: F
authors.lastname: Parillo
authors.forename: M P
authors.initials: MP
authors.lastname: Arias
authors.forename: A Verini
authors.initials: AV
authors.lastname: Supplizi
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D004240:Diverticulum / Q000473:pathology; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005064:Eustachian Tube / Q000166:cytology*; D005260:Female; D006651:Histocytochemistry; D006736:Horses / Q000033:anatomy & histology*; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D037102:Lectins; D009844:Oligosaccharides