title: A novel growth hormone 1 gene-derived probe for Oncorhynchus masou formosanus distinguished from the Oncorhynchus subspecies.
abstract: Traditional mitochondrial 16S rRNA is commonly used in many species identification studies. However, it is difficult to apply to the phylogenetic studies among the Oncorhynchus subspecies, which is a crucial need for management purposes for Oncorhynchus masou formosanus, Taiwan salmon. In this study, we have developed an improved species identification method for Taiwan salmon distinguished with other Oncorhynchus subspecies tested by exploiting PCR for growth hormone (GH) 1 gene. By comparing DNA sequences for GH1 from 11 species of Oncorhynchus subspecies we designed novel PCR primers that exploit differences between Taiwan salmon and other Oncorhynchus subspecies. Therefore, the technique is an important tool in the management of populations of the endangered land-locked Taiwan salmon preventing from their possible hybrids with other Oncorhynchus subspecies once tested.
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Jing-Iong
authors.initials: JI
authors.lastname: Yang
authors.forename: Hsiao-Yun
authors.initials: HY
authors.lastname: Huang
authors.forename: Jin-Chywan
authors.initials: JC
authors.lastname: Gwo
authors.forename: Ying-Fang
authors.initials: YF
authors.lastname: Su
authors.forename: Cheng-Hao
authors.initials: CH
authors.lastname: Wen
authors.forename: Yii-Cheng
authors.initials: YC
authors.lastname: Chou
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D017683:Oncorhynchus / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D029941:Fish Proteins; D013006:Growth Hormone