Detection of DNA by using bio-conducting polymer-Nile blue composite electrode; Nile blue as an indicator.
The amplified electrochemical sensing of DNA was accomplished by electrodeposited PEDOT on the electrode surface and incorporation of Nile blue (NB) as redox active intercalator into DNA. Herein, above modified electrode called as PEDOT/DNA/NB composite electrode. PEDOT/DNA/NB composite electrode exhibited well defined redox peak at -0.35 V (Ag/AgCl) corresponding to NB. The composite electrode surface coverage (Gamma) and DeltaEp were compared with PEDOT/NB and DNA/NB modified electrode. Atomic Force microscopy (AFM), and cyclic voltammetry (CV) were used to characterize the PEDOT/DNA/NB composite electrode. The composite electrode was exhibited as surface confined redox process in neutral pH. The composite electrode was found to be pH dependent. The composite electrode exhibited catalytic property towards reduction of hydrogen peroxide (H(2)O(2)). The composite electrode was utilized to amperometric study and its response towards H(2)O(2) detection was less than 6 s and the detection limit was 0.1 microM. Moreover, we tested PEDOT/DNA/NB composite electrode to electrocatalytic reduction of cytochrome c (Cyt c).
Chen
Zhi-Wei
ZW
Electroanalysis and Bioelectrochemistry lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road Taipei 106, Taiwan, ROC.
Balamurugan
A
A
Chen
Shen-Ming
SM
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000648:ultrastructure; D055664:Electrochemical Techniques; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010078:Oxazines / Q000032:analysis* / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
D019086:Bridged Bicyclo Compounds, Heterocyclic; D010078:Oxazines; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); C008619:Nile Blue; D045304:Cytochromes c; D004247:DNA; D006861:Hydrogen Peroxide