Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons.
Spectral properties of a recently developed voltage-sensitive dye, di-4-ANEPPDHQ, were characterized as the dye was dissolved in the solvent dimethyl sulfoxide as the stock solution, in Hank's buffered salt solution as the staining solution, and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons (GT1-7) in vitro. Their dependence on the local chemical and electrical environment of dye molecules was determined. The excitation and emission peaks are 479 nm and 570 nm for the stained primary neurons, and 476 nm and 585 nm for the stained immortalized neurons. The excitation and emission bands of the stained GT1-7 neurons, defined as 50% peak intensity, are 429-516 nm and 544-648 nm, respectively.
Wang
Yu
Y
Center for Optical Technologies, Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA 18015, USA. yuw207@lehigh.edu
Jing
Gaoshan
G
Perry
Susan
S
Bartoli
Filbert
F
Tatic-Lucic
Svetlana
S
D000818:Animals; D002461:Cell Line, Transformed; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry*; D004560:Electricity; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000166:cytology*; D011726:Pyridinium Compounds / Q000737:chemistry*; D051381:Rats; D012996:Solutions; D013057:Spectrum Analysis
D004396:Coloring Agents; D011726:Pyridinium Compounds; D012996:Solutions; C541550:di-4-ANEPPDHQ