The effect of addition of equine chorionic gonadotropin to a progesterone-based estrous synchronization protocol in buffaloes (Bubalus bubalis) under tropical conditions.
Poor estrus expression and anestrus decrease the reproductive efficiency of buffaloes. The objective of this study was to determine whether the addition of equine chorionic gonadotropin (eCG) to an estrous synchronization protocol and timed insemination could improve ovulation and pregnancy rates of anestrous buffalo cows under tropical conditions. The study population comprised 65 lactating Murrah buffalo cows which were assigned to CIDR (n=33) or CIDR+eCG (n=32) treatment groups. Cows in the CIDR group were fitted for 8d with a controlled intravaginal drug release (CIDR) device containing 1.38 g progesterone, received GnRH (10 microg i.m.) on D 0, PGF(2alpha) (750 microg i.m.) on D 7, and GnRH (10 microg i.m.) on D 9; whereas cows in the CIDR+eCG group received the same treatment plus eCG (500 IU, i.m.) at the time of PGF(2alpha) treatment. All cows were inseminated 16-20 h after the second GnRH treatment. Blood samples were obtained 10d before the start of synchronization treatment (Day -10) and at the onset of treatment (Day 0). Cows with plasma progesterone concentrations <1 ng/mL recorded in both samples (Low-Low levels of P4) were classified as non-cyclic cows. Similarly, when either one or both of the sample pair contained concentrations of serum progesterone >/=1 ng/mL (High-High, Low-High, or High-Low levels of P4), the buffaloes were classified as cyclic cows. Ovulation rate, defined as the number of buffaloes with at least one corpus luteum 10 days after insemination, was significantly higher (P=0.018) in the CIDR+eCG (84.4%) cows than in the CIDR cows (57.6%). Pregnancy rate was numerically lower in CIDR (27.3%) than CIDR+eCG (40.6%) cows, though differences were not significant (P=0.25). Pregnancy rates for CIDR+eCG cows were similar to that of cows inseminated after natural estrus (40.9%; 29/71). In the non-cyclic animals, higher ovulation rates (P=0.026) were recorded for the CIDR+eCG (81%) than for the CIDR cows (47.4%). Our results indicate that the addition of eCG to a progesterone-based estrous synchronization regimen substantially improves the ovulation rate in non-cyclic buffaloes. When this treatment is followed by timed AI, pregnancy rates achieved in anestrous buffaloes, whether cyclic and non-cyclic, may approach the rates observed in cows inseminated at natural estrus.
Murugavel
K
K
Department of Animal Reproduction, Gynaecology and Obstetrics, Rajiv Gandhi College of Veterinary and Animal Sciences, Kurumbapet, Pondicherry 605009, India.
Antoine
D
D
Raju
M S
MS
López-Gatius
F
F
D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003692:Delayed-Action Preparations; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*; D014329:Tropical Climate
D006063:Chorionic Gonadotropin; D003692:Delayed-Action Preparations; D011374:Progesterone