title: Centrotemporal sharp wave EEG trait in rolandic epilepsy maps to Elongator Protein Complex 4 (ELP4).
abstract: Rolandic epilepsy (RE) is the most common human epilepsy, affecting children between 3 and 12 years of age, boys more often than girls (3:2). Focal sharp waves in the centrotemporal area define the electroencephalographic (EEG) trait for the syndrome, are a feature of several related childhood epilepsies and are frequently observed in common developmental disorders (eg, speech dyspraxia, attention deficit hyperactivity disorder and developmental coordination disorder). Here we report the first genome-wide linkage scan in RE for the EEG trait, centrotemporal sharp waves (CTS), with genome-wide linkage of CTS to 11p13 (HLOD 4.30). Pure likelihood statistical analysis refined our linkage peak by fine mapping CTS to variants in Elongator Protein Complex 4 (ELP4) in two independent data sets; the strongest evidence was with rs986527 in intron 9 of ELP4, providing a likelihood ratio of 629:1 (P=0.0002) in favor of an association. Resequencing of ELP4 coding, flanking and promoter regions revealed no significant exonic polymorphisms. This is the first report of a gene implicated in a common focal epilepsy and the first human disease association of ELP4. ELP4 is a component of the Elongator complex, involved in transcription and tRNA modification. Elongator depletion results in the brain-specific downregulation of genes implicated in cell motility and migration. We hypothesize that a non-coding mutation in ELP4 impairs brain-specific Elongator-mediated interaction of genes implicated in brain development, resulting in susceptibility to seizures and neurodevelopmental disorders.
authors.affiliation: Child Health Evaluative Sciences, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Strug
authors.forename: Tara
authors.initials: T
authors.lastname: Clarke
authors.forename: Theodore
authors.initials: T
authors.lastname: Chiang
authors.forename: Minchen
authors.initials: M
authors.lastname: Chien
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Baskurt
authors.forename: Weili
authors.initials: W
authors.lastname: Li
authors.forename: Ruslan
authors.initials: R
authors.lastname: Dorfman
authors.forename: Bhavna
authors.initials: B
authors.lastname: Bali
authors.forename: Elaine
authors.initials: E
authors.lastname: Wirrell
authors.forename: Steven L
authors.initials: SL
authors.lastname: Kugler
authors.forename: David E
authors.initials: DE
authors.lastname: Mandelbaum
authors.forename: Steven M
authors.initials: SM
authors.lastname: Wolf
authors.forename: Patricia
authors.initials: P
authors.lastname: McGoldrick
authors.forename: Huntley
authors.initials: H
authors.lastname: Hardison
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Novotny
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: David A
authors.initials: DA
authors.lastname: Greenberg
authors.forename: James J
authors.initials: JJ
authors.lastname: Russo
authors.forename: Deb K
authors.initials: DK
authors.lastname: Pal
mesh_terms: D002648:Child; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D004252:DNA Mutational Analysis; D004569:Electroencephalography; D019305:Epilepsy, Rolandic / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013702:Temporal Lobe / Q000503:physiopathology
chemical_list: C456976:ELP4 protein, human; D009419:Nerve Tissue Proteins