Change of hydrolase activity in germinating seeds of trxS transgenic barley.
Genetic modification of barley variety can be an efficient way to improve beer quality. The objective of this study was to understand the effect of trxS gene on hydrolases activities in transgenic and non-transgenic barley seeds. The results showed that alpha-amylase, free beta-amylase and limit dextrinase activity were increased in transgenic seeds in comparison with non-transgenic seeds. Sulfhydryl content of protein in transgenic seeds was also higher than that in non-transgenic seeds, suggesting that trxS gene could express in barley seeds, which opens a new way for breeding new barley varieties to improve beer quality.
Wei
Li
L
Henan Agricultural University, Zhengzhou 450002, China.
Kong
Weiwei
W
Yin
Jun
J
D018525:Germination / Q000235:genetics; D005964:Glucosyltransferases / Q000378:metabolism; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D012639:Seeds / Q000201:enzymology* / Q000235:genetics; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics*; D000516:alpha-Amylases / Q000378:metabolism*; D001614:beta-Amylase / Q000378:metabolism
D013438:Sulfhydryl Compounds; D013879:Thioredoxins; D005964:Glucosyltransferases; C402863:dextrin dextranase; D000516:alpha-Amylases; D001614:beta-Amylase