[Inductive effect of RNAi RelB dendritic cells on the CD4(+)CD25(+) T cells proliferation].
OBJECTIVE
To discover the inductive proliferation effect of the RNAi RelB dendritic cells (DCs) on the heterogenic CD4(+)CD25(+) T cells in mice.


METHODS
The proportion of CD4(+)CD25(+) T cells were quantification through flow cytometer when each group of DCs interact with heterogenic T cells in mixed lymphocyte reaction, including control DCs, LPS stimulated DCs, RNAi RelB DCs and LPS stimulated RNAi RelB DCs.


RESULTS
Control DCs stimulate more CD4(+)CD25(+) T cells to proliferate than LPS stimulated DCs(P<0.01). RNAi RelB DCs and LPS stimulated RNAi RelB DCs significantly improved CD4(+)CD25(+) T cells proliferation compared with LPS stimulated DCs(P<0.01). RNAi RelB DCs can also induced CD4(+)CD25(+) T cells to proliferate more than Control DCs (P<0.05).


CONCLUSIONS
RNAi RelB DCs possess powerful potential to induce CD4(+)CD25(+) T cells proliferation, one of characteristics of immature dendritic cells. This type of RNAi RelB DCs can be used as tolerogenic DCs to regulate autoimmunity and other immune responses.
Zheng
Lei
L
Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Yan
Xiao-hui
XH
Wang
Qian
Q
Bao
Jie
J
Qiu
Yu-rong
YR
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D034622:RNA Interference / Q000502:physiology*; D051841:Transcription Factor RelB / Q000235:genetics*
D053645:Interleukin-2 Receptor alpha Subunit; D051841:Transcription Factor RelB