[Influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients].
OBJECTIVE
To study the influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients.


METHODS
The brucine liposomes were separated by Sephadex G-50, and the influence of various factors on the entrapment efficiencies were investigated.


RESULTS
The entrapment efficiency was enhanced by increased ammonium sulfate concentration, ethanol volume and PC concentration.


CONCLUSIONS
Burcine liposomes prepared by ammonium sulfate transmembrance gradients can get a high entrapment efficiency, the main influencing factors were ammonium sulfate concentration, ethanol volume and PC concentration.
Wang
Wei
W
Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Chen
Jun
J
Cai
Bao-Chang
BC
Fang
Yun
Y
D000645:Ammonium Sulfate / Q000737:chemistry*; D019540:Area Under Curve; D004337:Drug Carriers; D004355:Drug Stability; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D013331:Strychnine / Q000031:analogs & derivatives* / Q000737:chemistry; D031451:Strychnos / Q000737:chemistry*; D013678:Technology, Pharmaceutical; D013696:Temperature
D004337:Drug Carriers; D008081:Liposomes; D010743:Phospholipids; D000431:Ethanol; C083806:brucine; D013331:Strychnine; D000645:Ammonium Sulfate