Sequence-specific electrochemical detection of Alicyclobacillus acidoterrestris DNA using electroconductive polymer-modified fluorine tin oxide electrodes.
This study outlines the quantification of low levels of Alicyclobacillus acidoterrestris in pure cultures, since this bacterium is not inactivated by pasteurization and may remain in industrialized foods and beverages. Electroconductive polymer-modified fluorine tin oxide (FTO) electrodes and multiple nanoparticle labels were used for biosensing. The detection of A. acidoterrestris in pure cultures was performed by reverse transcription polymerase chain reaction (RT-PCR) and the sensitivity was further increased by asymmetric nested RT-PCR using electrochemical detection for quantification of the amplicon. The quantification of nested RT-PCR products by Ag/Au-based electrochemical detection was able to detect 2 colony forming units per mL (CFU mL(-1)) of spores in pure culture and low detection and quantification limits (7.07 and 23.6 nM, respectively) were obtained for the target A. acidoterrestris on the electrochemical detection bioassay.
Barrios Eguiluz
Katlin Ivon
KI
GMEME - Grupo de Materiais Eletroquímicos e Métodos Eletroanalíticos, Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense, 400, C.P. 780, 13560-970, São Carlos - SP, Brazil.
Salazar-Banda
Giancarlo Richard
GR
Funes-Huacca
Maribel Elizabeth
ME
Alberice
Juliana Vieira
JV
Carrilho
Emanuel
E
Spinola Machado
Sergio Antonio
SA
Avaca
Luis Alberto
LA
D001406:Bacillaceae / Q000235:genetics*; D001628:Beverages / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005461:Fluorine; D005516:Food Microbiology; D005638:Fruit; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017971:Tin Compounds
D004269:DNA, Bacterial; D017971:Tin Compounds; D005461:Fluorine; C045358:stannic oxide