[Morphological observation on the reproductive system of adult Schistosoma japonicum under confocal laser scanning microscopy].
BALB/c mice were infected with Schistosoma japonicum cercariae (40+/-2 per mouse) through abdominal skin. Mice were sacrificed after 35 days to acquire the adult worms which were then fixed, stained, clarified, dehydrated and mounted. The specimens were observed under the confocal laser scanning microscope. The overall morphology of the adult worms was displayed distinctly, especially the testicular lobes, seminal vesicle and genital pore of the male, reproductive system, and the ovary, vitelline glands, oviduct, vitelline duct, seminal receptacle, ootype, mehlis gland, uterus, genital pore and eggs of female reproductive system. The confocal laser scanning microscopy is an alternative method to research organs, tissues and cell structure of schistosome worm.
Zhang
Wei-Na
WN
Department of Microbiology and Parasitology, Anhui Medical University, Hefei 230032, China.
Huang
Da-Ke
DK
Zhang
Peng
P
Ren
Cui-Ping
CP
Wang
Xiao-Nan
XN
Zhang
Yun-Xia
YX
Shen
Ji-Jia
JJ
D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D012549:Schistosoma japonicum / Q000033:anatomy & histology*; D012554:Schistosomiasis japonica / Q000469:parasitology; D014566:Urogenital System / Q000033:anatomy & histology*