Zonula occludens-1, occludin, and E-cadherin protein expression in biliary tract cancers.
The incidence of cholangiocarcinomas originating from intrahepatic and extrahepatic bile ducts, as well as of gallbladder carcinoma is increasing worldwide. The malignant transformation of biliary epithelia involves profound alterations of proteins in the intercellular junctions, among others zonula occludens-1 (ZO-1), occludin, and E-cadherin. Each plays important role in the maintenance of epithelial cell polarity and regulation of cell growth and differentiation. Our aim was to investigate ZO-1, occludin, and E-cadherin immunohistochemical reactions in tissue microarray blocks containing 57 normal and 62 neoplastic biliary tract samples. We demonstrated that the tight junction components ZO-1, occludin, and E-cadherin are downregulated in carcinomas arising from various compartments of the biliary tract (normal intrahepatic and extrahepatic bile ducts, gallbladder) as compared with their normal sites of origin. These results were confirmed by discriminant analysis yielding clear separation of the three normal sample groups from carcinomas in the corresponding locations.
Németh
Zsuzsanna
Z
2nd Department of Pathology, Semmelweis University, Ulloi út 93, H-1091 Budapest, Hungary.
Szász
Attila Marcell
AM
Somorácz
Aron
A
Tátrai
Péter
P
Németh
Júlia
J
Gyorffy
Hajnalka
H
Szíjártó
Attila
A
Kupcsulik
Péter
P
Kiss
András
A
Schaff
Zsuzsa
Z
D000368:Aged; D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D015820:Cadherins / Q000096:biosynthesis*; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D062793:Occludin; D010750:Phosphoproteins / Q000096:biosynthesis*; D046888:Tissue Array Analysis; D062826:Zonula Occludens-1 Protein
D014408:Biomarkers, Tumor; D015820:Cadherins; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D010750:Phosphoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein