title: A quantitative peptidomic analysis of peptides related to the endogenous opioid and tachykinin systems in nucleus accumbens of rats following naloxone-precipitated morphine withdrawal.
abstract: We have applied a recently developed label-free mass spectrometry based peptidomic approach to identify and quantify a variety of endogenous peptides from rat nucleus accumbens following withdrawal in naloxone-precipitated, morphine-dependent rats of two separate strains. We focused on maturated, partially processed and truncated peptides derived from the peptide precursors proenkephalin, prodynorphin and preprotachykinin. The expression of several identified peptides was dependent on strain and was affected during morphine withdrawal.
authors.affiliation: Department of Pharmaceutical Biosciences, Uppsala University, SE-751 24, Uppsala, Sweden.
authors.forename: Uwe
authors.initials: U
authors.lastname: Rossbach
authors.forename: Anna
authors.initials: A
authors.lastname: Nilsson
authors.forename: Maria
authors.initials: M
authors.lastname: Fälth
authors.forename: Kim
authors.initials: K
authors.lastname: Kultima
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Mathias
authors.initials: M
authors.lastname: Hallberg
authors.forename: Torsten
authors.initials: T
authors.lastname: Gordh
authors.forename: Per E
authors.initials: PE
authors.lastname: Andren
authors.forename: Fred
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D000595:Amino Acid Sequence; D000701:Analgesics, Opioid / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009020:Morphine / Q000378:metabolism*; D009270:Naloxone / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000737:chemistry* / Q000378:metabolism; D018847:Opioid Peptides / Q000032:analysis*; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013375:Substance Withdrawal Syndrome; D015320:Tachykinins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000701:Analgesics, Opioid; D018847:Opioid Peptides; D011498:Protein Precursors; D020543:Proteome; D015320:Tachykinins; D009270:Naloxone; D009020:Morphine