title: Ileal mucosal and fecal pancreatitis associated protein levels reflect severity of salmonella infection in rats.
abstract: BACKGROUND
  Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection.
  
  
  RESULTS
  Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces.
  
  
  CONCLUSIONS
  PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker.
authors.affiliation: TI Food and Nutrition, Wageningen, The Netherlands.
authors.forename: Marleen T J
authors.initials: MT
authors.lastname: van Ampting
authors.forename: Wendy
authors.initials: W
authors.lastname: Rodenburg
authors.forename: Carolien
authors.initials: C
authors.lastname: Vink
authors.forename: Evelien
authors.initials: E
authors.lastname: Kramer
authors.forename: Arjan J
authors.initials: AJ
authors.lastname: Schonewille
authors.forename: Jaap
authors.initials: J
authors.lastname: Keijer
authors.forename: Roelof
authors.initials: R
authors.lastname: van der Meer
authors.forename: Ingeborg M J
authors.initials: IM
authors.lastname: Bovee-Oudenhoven
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D018988:Bacterial Translocation; D015415:Biomarkers / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002136:Calcium, Dietary / Q000378:metabolism; D004195:Disease Models, Animal; D004435:Eating; D020895:Enterocytes / Q000378:metabolism / Q000382:microbiology; D005243:Feces / Q000737:chemistry* / Q000382:microbiology; D007079:Ileitis / Q000378:metabolism / Q000382:microbiology; D007082:Ileum / Q000378:metabolism* / Q000382:microbiology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008297:Male; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012481:Salmonella Infections, Animal / Q000378:metabolism* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000472:pathogenicity*; D012720:Severity of Illness Index; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; D015415:Biomarkers; D014408:Biomarkers, Tumor; D002136:Calcium, Dietary; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D012333:RNA, Messenger; C000612144:Reg3b protein, rat