Phosphatidylinositol 3-kinase activity is required for the induction of differentiation in C6 glioma cells by panaxydol.
Panaxydol isolated from the lipophilic fractions of Tienchi ginseng (Panax notoginseng) induces growth inhibition and differentiation of rat C6 glioma cells. The underlying molecular mechanisms are not completely understood. In the present study, we identified phosphatidylinositol 3-kinase (PI 3-K) as a necessary enzyme for the differentiation of C6 cells treated with panaxydol. The specific PI 3-K inhibitor wortmannin resulted in attenuated differentiation of C6 cells induced by panaxydol, and was associated with perinuclear localization of glial fibrillary acidic protein expression and a diminished process formation. These data suggest that induction of differentiation in C6 cells by panaxydol could be mediated through a PI 3-K-dependent pathway.
Hai
Jian
J
Department of Neurosurgery, Tongji Hospital, Tongji University, 389 Xin-Cun Road, Shanghai 200065, China. haijiandoc@yahoo.com.cn
Lin
Qi
Q
Lu
Yang
Y
D000704:Analysis of Variance; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D053280:Diynes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005233:Fatty Alcohols / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005910:Glioma / Q000503:physiopathology; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051381:Rats; D000077191:Wortmannin
D000730:Androstadienes; D053280:Diynes; D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D005904:Glial Fibrillary Acidic Protein; C055778:panaxydol; D019869:Phosphatidylinositol 3-Kinases; D000077191:Wortmannin