A dual-functional E. coli vector for expressing recombinant protein with high solubility and antigen presentation ability.
A dual-functional Escherichia coli expression vector capable of producing soluble recombinant proteins with high immunogenicity in animals is introduced. This vector expresses polypeptides fused to a PTD-J-domain peptide. The J-domain peptide is derived from murine Hsp40 by using optimized codons for E. coli. The association of the J-domain to the nucleotide binding domain of the DnaK chaperone increases the probability that the fused polypeptide will be folded by the DnaK and hence increases the solubility of the recombinant protein. The PTD-J-domain can also enhance the immunogenicity of the fused chicken IGF-I polypeptide as well as an oligo-peptide derived from haptoglobin in rodents, possibly via the association with either the extracellular or intracellular Hsp70 proteins.
Chuang
Chin-Kai
CK
Division of Biotechnology, Animal Technology Institute Taiwan, No. 52, Kedung 2nd Rd., Chunan 35053, Miaoli, Taiwan. jkjuang@mail.atit.org.tw
Su
Yu-Show
YS
Fan
Chiu-Tin
CT
Lee
Wen-Chuan
WC
Chen
Ming-Yu
MY
D000818:Animals; D017951:Antigen Presentation; D000941:Antigens / Q000096:biosynthesis* / Q000235:genetics; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005822:Genetic Vectors; D050956:HSP40 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D017434:Protein Structure, Tertiary / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D012995:Solubility
D000941:Antigens; C494641:Dnajb1 protein, mouse; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D007334:Insulin-Like Growth Factor I; C063842:dnaK protein, E coli