title: Effect of Bifidobacterium bifidum DSM 20082 cytoplasmic fraction on human immune cells.
abstract: The objective of the present study was to determine the effect of the soluble cytoplasmic fraction from Bifidobacterium bifidum DSM 20082 (Bb) lysate on peripheral blood T cells. In peripheral blood mononuclear cells of healthy subjects, cytotoxic activity, proliferation, apoptosis, and up-regulation of CD8 or CD4 molecules in T cells were examined. When peripheral blood mononuclear cells were stimulated with Bb lysate, the main effect was observed in CD8+ cells as a significant increase of CD8 molecules in a dose-dependent manner, and this behavior was observed at 24, 48, and 72 h after stimulation; in contrast, stimulation with Bb lysate showed no effect on the up-regulation of CD4 molecules in T helper cells. Further Bb lysate did not induce proliferation activity in either CD8+ or CD4+ cells. Bb lysate induced activation of CD8+ cytotoxic activity against autologous monocytes. Around 80% of the cells stimulated with Bb lysate were positive to peanut agglutinin (PNA), suggesting that the stimulated CD8+ cells corresponded to activated/effector cellular populations. When apoptosis was determined, there were no differences between stimulated and non-stimulated cells. Our results indicate that Bb lysate is able to increase cytotoxic activity of peripheral CD8+ cells, without affecting lymphocyte survival.
authors.affiliation: Universite de Sciences et Technologies de Lille, France.
authors.forename: Fadoua
authors.initials: F
authors.lastname: Mouni
authors.forename: Eliane
authors.initials: E
authors.lastname: Aissi
authors.forename: Jesus
authors.initials: J
authors.lastname: Hernandez
authors.forename: Patricia
authors.initials: P
authors.lastname: Gorocica
authors.forename: Stephane
authors.initials: S
authors.lastname: Bouquelet
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lascurain
authors.forename: Yonathan
authors.initials: Y
authors.lastname: Garfias
mesh_terms: D017209:Apoptosis / Q000276:immunology; D001644:Bifidobacterium / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016827:CD8 Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002458:Cell Fractionation; D049109:Cell Proliferation; D002469:Cell Separation; D003593:Cytoplasm / Q000276:immunology / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry; D006801:Humans; D019887:Peanut Agglutinin / Q000378:metabolism; D028321:Plant Preparations / Q000494:pharmacology
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D019887:Peanut Agglutinin; D028321:Plant Preparations