Nitric oxide metabolites, leukocyte activation markers and oxidative status in dialyzed subjects.
OBJECTIVE
Our purpose was to evaluate, in a group of 42 end-stage renal disease patients who regularly undergo hemodialysis, some indexes of leukocyte activation, nitric oxide metabolites (NOx) and other parameters that reflect the oxidative stress before and after a standard hemodialysis session.


METHODS
Elastase and myeloperoxidase (MPO) were determined by means of ELISA. The NO production was evaluated by a micromethod which measures the concentration of NOx. The oxidation of polyunsaturated fatty acids was evaluated in plasma by detection of thiobarbituric acid-reactive substances (TBARS). Total antioxidant status (TAS) was obtained using spectrophotometry.


RESULTS
At baseline, we observed an increase of elastase, NOx, TBARS and TAS, without any variation of MPO. After the dialysis session, we found an increase in elastase and MPO, a decrease in NOx and TAS and no variation in TBARS. No modification occurred after subdividing the patients in two subgroups.


CONCLUSIONS
Our data confirm the involvement of leukocytes and oxidative stress in hemodialysis patients.
Caimi
G
G
Dipartimento di Medicina Interna, Malattie Cardiovascolari e Nefrourologiche, Università di Palermo, Palermo, Italy. caimigre@unipa.it
Carollo
C
C
Montana
M
M
Iatrino
R
R
Bondì
B
B
Lo Presti
R
R
D000368:Aged; D000975:Antioxidants / Q000032:analysis; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D019272:Leukocyte Elastase / Q000097:blood; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D006435:Renal Dialysis / Q000009:adverse effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D009195:Peroxidase; D019272:Leukocyte Elastase