PrP(Sc) of scrapie 263K propagates efficiently in spleen and muscle tissues with protein misfolding cyclic amplification.
Transmissible spongiform encephalopathies (TSEs), or prion diseases, are transmissible neurodegenerative disorders of protein conformation. This group of diseases is caused by infectious agents, termed prions, which can convert normal conformation (PrP(C)) into misfolded protein (PrP(Sc)). The infectivity of non-neuronal tissues has been wildly addressed, but the propagating features and the biochemical properties of prion generated from these tissues are only partially settled. In this study, utilizing protein misfolding cyclic amplification (PMCA), the in vitro conversion of PrP(C) into PrP(Sc) in spleen and muscle tissues can be induced by PrP(Sc) produced in vivo. The further propagation of newly formed PrP(Sc) in normal brain and some of the biochemical properties of new PrP(Sc) are similar as the brain-derived prions, implying the naturally infectious pathway of prion from peripheral generation to neuro-invasion. However, compared with the brain-derived PrP(Sc), the weaker resistance of new PrP(Sc) to some inactivated agents, i.e. sodium hydroxide and thermal inactivation, are observed. Our data provide the reliable evidence that the brain-derived PrP(Sc) can utilize the PrP(C) from non-neuronal tissues for its propagation. Similarity of the replicative ability in PMCA in vitro and the infectivity in vivo highlights the possibility to use PMCA instead of bioassay to investigate the propagation of prion.
Shi
Song
S
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Ying-Xin Rd 100, Beijing 100052, People's Republic of China.
Dong
Chen-Fang
CF
Wang
Gui-Rong
GR
Wang
Xin
X
An
Run
R
Chen
Jian-Ming
JM
Shan
Bing
B
Zhang
Bao-Yun
BY
Xu
Kun
K
Shi
Qi
Q
Tian
Chan
C
Gao
Chen
C
Han
Jun
J
Dong
Xiao-Ping
XP
D000818:Animals; D006224:Cricetinae; D006801:Humans; D009132:Muscles / Q000737:chemistry / Q000378:metabolism*; D018621:PrPC Proteins / Q000737:chemistry / Q000378:metabolism; D018620:PrPSc Proteins / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding; D012608:Scrapie / Q000378:metabolism*; D013154:Spleen / Q000737:chemistry / Q000378:metabolism*
D018621:PrPC Proteins; D018620:PrPSc Proteins