title: Detection of mycoestrogen zearalenone by a molecularly imprinted polypyrrole-based surface plasmon resonance (SPR) sensor.
abstract: The aim of the present work was to investigate the feasibility of employing the molecular imprinting polymer technique for detecting the mycotoxin zearalenone using a surface plasmon resonance (SPR) transducer. The molecularly imprinted polypyrrole (MIPPy) film was prepared by electropolymerization of pyrrole onto the bare Au chip in the presence of a template zearalenone molecule. The MIPPy-SPR sensor exhibited a linear response in the range of 0.3-3000 ng/mL (R (2) = 0.993) for detection of zearalenone. The selectivity efficiencies of zearalenone and other structurally related analogues were 1.0 and 0.15-0.27, respectively. The limit of detection and average recovery of blank corn matrix spiked with 30 ng/g zearalenone were 0.3 ng/g and 89%, respectively, and these were found to be comparable to those obtained by enzyme-linked immunosorbent assay. These results suggest that a combination of SPR sensing with MIPPy film is a promising alternative method for the detection of zearalenone.
authors.affiliation: Korea Food Research Institute, San 516, Backhyun-dong, Bundang-gu, Sungnam, Kyonggi-do 463-746, Korea.
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Choi
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Chang
authors.forename: Nari
authors.initials: N
authors.lastname: Lee
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Hyang Sook
authors.initials: HS
authors.lastname: Chun
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D054802:Molecular Imprinting; D011108:Polymers; D011758:Pyrroles; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D015025:Zearalenone / Q000032:analysis*
chemical_list: D004968:Estrogens, Non-Steroidal; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D015025:Zearalenone