Characterization of the endonuclease SSO2001 from Sulfolobus solfataricus P2.
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes (cas genes) have been suggested to act as an immune system in archaea and bacteria mimicking the eukaryotic RNA interference (RNAi) system. We have investigated the properties of the protein SSO2001 from Sulfolobus solfataricus (Sso) P2, which is part of the cas gene cluster. This study shows that SSO2001 is an endonuclease specifically digesting double-stranded oligonucleotides and preferably cleaving at G:C pairs. Point mutations identify both highly conserved aspartate and glutamate residues as being crucial for the nuclease activity. The catalytic activity shows an optimum at neutral pH and pH 3.
Han
Dong
D
Department of Biochemistry, Universität Bayreuth, Universitätsstrasse 30, Bayreuth, 95447, Germany.
Krauss
Gerhard
G
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D017124:Conserved Sequence; D019641:DNA, Archaeal / Q000235:genetics; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D005810:Multigene Family; D017354:Point Mutation; D011485:Protein Binding / Q000235:genetics; D011506:Proteins / Q000235:genetics; D019642:RNA, Archaeal / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D019843:Archaeal Proteins; D019641:DNA, Archaeal; D011506:Proteins; D019642:RNA, Archaeal; D004720:Endonucleases