Blocking glutamate receptors in the waist area of the parabrachial nucleus decreases taste reactivity behaviors in conscious rats.
The "waist" area (W) of the parabrachial nucleus contains neurons that receive orosensory input and play a role in the initiation of oromotor behaviors. Immunohistochemical data indicate that neurons in W receive glutamatergic input and express glutamate receptors, but a behavioral role for glutamate neurotransmission within W has not been investigated. To determine the role of specific glutamate receptors in taste reactivity behaviors, glutamate receptor blockers were delivered into W by reverse microdialysis during intraoral infusion of 0.1 M sodium chloride, 0.1 M sucrose, 0.03 M hydrochloric acid, and 0.003 M quinine hydrochloride. Blocking alpha-amino-3-hydroxy-5-methyl-isoxazolepropionate (AMPA)/kainate ionotropic glutamate receptors in W with 6-cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX) reduced ingestive taste reactivity behaviors to each tastant by 72-85% compared with baseline levels (P's < 0.05). Blocking N-methyl-D-aspartate receptors as well as type 1 and group III metabotropic glutamate receptors had minor effects on taste reactivity responses to the tastants. These data provide strong evidence for a behavioral role of glutamatergic neurotransmission in W in conscious rats.
Biondolillo
Joseph W
JW
Biology Department, Stetson University, DeLand, FL 32723, USA.
Williams
Learnel A
LA
King
Michael S
MS
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000601:surgery; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology; D013649:Taste / Q000187:drug effects / Q000502:physiology*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D007608:Kainic Acid