Sensitivity enhancement in tandem liquid chromatographic mass spectrometric assays by summation of two transition ion pairs--perspectives.
The introduction of tandem liquid chromatographic mass spectrometric assays has boosted the sensitivity of the bioanalytical determination for the scores of analytes as compared to other conventional modes of detection [1-6]. Additionally, many intelligent variants such as improved ionization potential, suitable adduct formation, and/or identification of a precursor ion, switching of modality of detection (+ve to -ve and vice versa), and derivatization steps (to aid fragmentation and/or impart ionization property) within tandem mass spectrometric assays have further aided in improving the sensitivity. Some researchers have also expressed thoughts of using matrix effects in a beneficial manner so that there may be ion enhancement for improving the sensitivity of the assay.
Srinivas
Nuggehally R
NR
Integrated Drug Development, Suramus Biopharm, J.P. Nagar I Phase, Bangalore, India. srini.suramus@yahoo.com
D002853:Chromatography, Liquid / Q000379:methods*; D007477:Ions; D017711:Nonlinear Dynamics; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D007477:Ions