Toll-like receptor-2, CD14 and heat-shock protein 70 in inflammatory lesions of rat experimental autoimmune neuritis.
Experimental autoimmune neuritis (EAN) is a well-known animal model of Guillain-Barré syndrome (GBS) characterized by inflammation and demyelination in the peripheral nervous system. Toll-like receptors (TLRs) together with their co-receptors form the first line of the self-defense, and play important roles in innate immune responses and inflammation. TLRs can be activated by endogenous ligands, like heat shock protein 70 (HSP70). In this study, we examined the spatiotemporal expressions of TLR2, CD14 and Hsp70 in EAN rats using immunohistochemistry and RT-PCR. A significant up-regulation of TLR2, CD14 and Hsp70 was seen in sciatic nerves of EAN rats and correlated with disease severity. Furthermore, activated macrophages were the main cellular resource of TLR2, CD14 and Hsp70 in EAN. Our results suggest that TLR2-, CD14- or Hsp70-based immunomodulation might have potential in the control of unwanted innate immune system activation in inflammatory neuropathies.
Zhang
Z-Y
ZY
Institute of Brain Research, University of Tuebingen, Calwer Street 3, D-72076, Tuebingen, Germany.
Zhang
Z
Z
Schluesener
H J
HJ
D000818:Animals; D004195:Disease Models, Animal; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D004676:Myelin Basic Protein / Q000008:administration & dosage; D009444:Neuritis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000378:metabolism; D013997:Time Factors; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000276:immunology / Q000502:physiology*
D018840:HSP70 Heat-Shock Proteins; D018950:Lipopolysaccharide Receptors; D004676:Myelin Basic Protein; D012333:RNA, Messenger; C495343:Tlr2 protein, rat; D051195:Toll-Like Receptor 2; D018664:Interleukin-12