Mycoflora and mycotoxin production in oilseed cakes during farm storage.
Agricultural activities involve the use of oilseed cakes as a source of proteins for livestock. Because the storage of oilseed cakes could induce the development of molds and the production of mycotoxins, a survey was conducted during the 5 months of farm storage. Mycoflora was studied by microscopic examinations, and the presence of Aspergillus fumigatus was confirmed by polymerase chain reaction. A multimycotoxin method was developed to quantify seven mycotoxins (aflatoxin B(1), alternariol, fumonisin B(1), gliotoxin, ochratoxin A, T-2 toxin, and zearalenone) in oilseed cakes by high-performance liquid chromatography coupled to mass spectrometry. Among 34 fungal species identified, A. fumigatus and Aspergillus repens were observed during 5 and 4 months, respectively. Gliotoxin, an immunosuppressive mycotoxin, was quantified in oilseed cakes up to 45 microg/kg, which was associated with the presence of toxigenic isolates of A. fumigatus.
Lanier
Caroline
C
Groupe Régional d'Etudes sur le Cancer, GRECAN-EA 1772, IFR 146 ICORE, Université de Caen Basse-Normandie, Centre François Baclesse, avenue Général Harris, BP 5026, 14076 Caen Cedex 05, France.
Heutte
Natacha
N
Richard
Estelle
E
Bouchart
Valerie
V
Lebailly
Pierre
P
Garon
David
D
D000821:Animal Feed / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification; D029688:Brassica napus; D002851:Chromatography, High Pressure Liquid; D005519:Food Preservation; D006368:Helianthus; D009183:Mycotoxins / Q000032:analysis* / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000382:microbiology*; D013025:Glycine max
D009183:Mycotoxins