Effects of dentin bonding agents on the cell cycle of fibroblasts.
The aim of this study was to evaluate the effects of 3 dentin bonding agents on cell survival and proliferation and on cell cycle progression of cultured cells. The experiments were performed on RPC-C2A and L929 cells. Specimens of the 3 dentin bonding agents (Clearfil Tri-S, AdheSE, and XP BOND) were placed in culture medium, and the extraction media were applied to cells as experimental material. The effect of the bonding materials on cell survival and proliferation was assessed by a modified sulforhodamine B staining assay, and the effect on DNA synthesis was assessed by bromodeoxyuridine uptake. Flow cytometry was used for cell cycle analysis. Cell viability and proliferation decreased in a dose-dependent manner after exposure of cells to the tested materials. XP BOND expressed the highest activity of all tested bonding agents (P < .05). The self-etch bonding agents tested did not produce any significant effects on cell cycle distribution. However, exposure of cells to the total-etch agent XP BOND induced a G(2)-phase arrest in both cell lines, and this effect was more evident in L929 cells than in RPC-C2A cells.
Koulaouzidou
Elisabeth A
EA
Department of Operative Dentistry, Dental School, Aristotle University, Thessaloniki, Greece. koulaouz@dent.auth.gr
Papazisis
Konstantinos T
KT
Yiannaki
Efi
E
Palaghias
Georgios
G
Helvatjoglu-Antoniades
Maria
M
D000180:Acrylic Resins / Q000633:toxicity; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects*; D007739:L Cells; D008422:Materials Testing; D051379:Mice; D051381:Rats; D019279:Resin Cements / Q000633:toxicity*
D000180:Acrylic Resins; C489662:AdheSE cement; C515604:Clearfil Tri-S Bond; D017220:Dentin-Bonding Agents; D019279:Resin Cements; C532387:XP-Bond; D004247:DNA