title: Characterization of murine MGL1 and MGL2 C-type lectins: distinct glycan specificities and tumor binding properties.
abstract: Antigen presenting cells (APC) express a variety of pattern recognition receptors, including the C-type lectin receptors (CLR) that specifically recognize carbohydrate structures expressed on self-tissue and pathogens. The CLR play an important role in antigen uptake and presentation and have been shown to mediate cellular interactions. The ligand specificity of the human macrophage galactose-type lectin (MGL) has been characterized extensively. Here, we set out to determine the glycan specificity of the murine homologues, MGL1 and MGL2, using a glycan array. Murine MGL1 was found to be highly specific for Lewis X and Lewis A structures, whereas mMGL2, more similar to the human MGL, recognized N-acetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen and core 2. The generation of MGL1 and MGL2-Fc proteins allowed us to identify ligands in lymph nodes, and MGL1-Fc additionally recognized high endothelial venules. Strikingly, MGL2 interacted strongly to adenocarcinoma cells, suggesting a potential role in tumor immunity.
authors.affiliation: Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Satwinder Kaur
authors.initials: SK
authors.lastname: Singh
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Streng-Ouwehand
authors.forename: Manja
authors.initials: M
authors.lastname: Litjens
authors.forename: Danny R
authors.initials: DR
authors.lastname: Weelij
authors.forename: Juan Jesús
authors.initials: JJ
authors.lastname: García-Vallejo
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: van Vliet
authors.forename: Eirikur
authors.initials: E
authors.lastname: Saeland
authors.forename: Yvette
authors.initials: Y
authors.lastname: van Kooyk
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001212:Asialoglycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008024:Ligands; D008198:Lymph Nodes / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism
chemical_list: D001212:Asialoglycoproteins; C064720:Clec10a protein, mouse; D037181:Lectins, C-Type; D008024:Ligands; C464940:MGL2 protein, mouse; D008565:Membrane Proteins; D011134:Polysaccharides; D011994:Recombinant Proteins