Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis.
OBJECTIVE
Major surgery can modulate the immune system and by this the clinical course of following complications. Effects of minor surgical treatments on the immune system and septic complications are poorly understood.


METHODS
We investigated the effect of a minor surgical procedure--the implantation of an osmotic pump--on the outcome of experimental polymicrobial sepsis (colon ascendens stent-induced peritonitis, CASP) in mice.


RESULTS
Animals with pumps implanted 3 days prior to CASP showed an attenuated clinical course of sepsis and increased survival. While measured serum cytokine levels were not affected by the minor surgical stress of pump implantation, splenocyte secretion of IFN-gamma in response to lipopolysaccharide was increased.


CONCLUSIONS
The early implantation of alloplastic material modulates the immune system and leads to an increased survival of a polymicrobial sepsis. Identifying the molecular nature of this effect might point the way to a new therapeutic approach to reduce sepsis mortality.
Kessler
Wolfram
W
Klinik für Allgemeine Chirurgie, Visceral-, Thorax- und Gefässchirurgie der Ernst-Moritz-Arndt-Universität Greifswald, Friedrich-Loeffler-Str. 23b, 17457, Greifswald, Germany. wkessler@uni-greifswald.de
Ebker
Tobias
T
Koerner
Pia
P
Poetschke
Christian
C
Cziupka
Katharina
K
Traeger
Tobias
T
Westerholt
Alexandra
A
Mehmcke
Hendrik
H
Neher
Friederike
F
Jack
Robert S
RS
Heidecke
Claus-Dieter
CD
Maier
Stefan
S
D000818:Animals; D018925:Chemokines / Q000097:blood / Q000276:immunology; D044682:Colon, Ascending; D016207:Cytokines / Q000097:blood / Q000276:immunology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D007121:Immunocompetence / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000517:prevention & control; D019919:Prosthesis Implantation; D018805:Sepsis / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control*; D012720:Severity of Illness Index; D016037:Single-Blind Method; D013154:Spleen / Q000166:cytology; D018709:Statistics, Nonparametric; D015607:Stents; D015996:Survival Rate; D013997:Time Factors; D014628:Vagotomy
D018925:Chemokines; D016207:Cytokines; D008070:Lipopolysaccharides