Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase.
Metagenomics, a new research field developed over the past decade, aims to identify potential enzymes from nonculturable microbes. In this study, genes encoding three glycoside hydrolase family (GHF) 9 endoglucanases and one GHF 5 endoglucanase were cloned and identified from the metagenome of the compost soils. The shared identities between the predicted amino acid sequences of these genes and their closest homologues in the database were less than 70%. One GHF 9 endoglucanase, Umcel9B, was further characterized. The recombinant protein, Umcel9B, showed activity against carboxymethyl cellulose, indicating that Umcel9B is an endoactive enzyme. Enzymatic activity occurs optimally at a pH of 7.0 and a temperature of 25 degrees C.
Pang
Hao
H
Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, People's Republic of China.
Zhang
Peng
P
Duan
Cheng-Jie
CJ
Mo
Xin-Chun
XC
Tang
Ji-Liang
JL
Feng
Jia-Xun
JX
D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D016678:Genome; D015698:Genomic Library; D023281:Genomics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
D011994:Recombinant Proteins; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium