Transcription factor TBX1 overexpression induces downregulation of proteins involved in retinoic acid metabolism: a comparative proteomic analysis.
TBX1 haploinsufficiency is considered a major contributor to the del22q11.2/DiGeorge syndrome (DGS) phenotype. We have used proteomic tools to look at all the major proteins involved in the TBX1-mediated pathways in an attempt to better understand the molecular interactions instrumental to its cellular functions. We found more than 90 proteins that could be targeted by TBX1 through different mechanisms. The most interesting observation is that overexpression of TBX1 results in down-regulation of two proteins involved in retinoic acid metabolism.
Caterino
Marianna
M
CEINGE Biotecnologie Avanzate scarl, Napoli, Italy, Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Napoli, Italy.
Ruoppolo
Margherita
M
Fulcoli
Gabriella
G
Huynth
Tuong
T
Orrù
Stefania
S
Baldini
Antonio
A
Salvatore
Francesco
F
D000818:Animals; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D051379:Mice; D020543:Proteome / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014212:Tretinoin / Q000378:metabolism*
D020543:Proteome; D020825:T-Box Domain Proteins; C491573:Tbx1 protein, mouse; D014212:Tretinoin