Construction of anticoagulant poly (lactic acid) films via surface covalent graft of heparin-carrying microcapsules.
Heparin has been widely investigated in medicine and biomedical applications as an anticoagulant. This paper described a covalent modification of poly (lactic acid) (PLA) films with heparin-carrying microcapsules. The heparin-carrying microcapsules were achieved by layer-by-layer (LbL) self-assembly technology by which poly (allylamine hydrochloride) (PAH) and heparin were alternately coated on the Ca(2+)-cross-linked alginate hydrogel. The microcapsules were characterized by scanning electron microscopy (SEM), zeta potential analysis and Fourier transform infrared spectroscopy (FTIR), and then grafted to PLA films by -CONH- linkage between surface PLA molecules with residual primary amino groups on the outer PAH layer of microcapsules, in the presence of catalysts of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). SEM and FTIR revealed the formation of -CONH- linkage. In vitro antithrombogenicity by the method of the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) tests showed that surface-modified PLA films had superior coagulation properties to original PLA films. It is suggested that the present method would provide a potential effective tool for biomaterial modification.
Li
Juan
J
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
Zhu
Baoqing
B
Shao
Yingya
Y
Liu
Xinrong
X
Yang
Xinlin
X
Yu
Qun
Q
D000925:Anticoagulants / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002214:Capsules / Q000737:chemistry*; D004337:Drug Carriers; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D010314:Partial Thromboplastin Time; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011517:Prothrombin Time; D017550:Spectroscopy, Fourier Transform Infrared; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties; D013918:Thrombin Time
D000925:Anticoagulants; D002214:Capsules; D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; D013388:Succinimides; D019344:Lactic Acid; C033616:poly(lactide); D006493:Heparin; C001426:N-hydroxysuccinimide; D002118:Calcium