Control over micro-fluidity of liposomal membranes by hybridizing metal nanoparticles.
This study introduces a facile method to hybridize metal nanoparticles with lipid vesicles, which allows us to control over their membrane micro-fluidity. We have fabricated these hybrid liposomes by directly hybridizing metal nanoparticles with lipid bilayers solely consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). For this, we have used the dehydration and rehydration method. Characterizing their morphology and micro-fluidity, in which we have used electron microscopy and fluorescence anisotropy spectroscopy, enables us to demonstrate that metal nanoparticles with different surface properties create interactions with either phosphorus end groups or hydrophobic tails of DPPC, thereby resulting in decrease in micro-fluidity of the assembled lipid membranes, especially for the hydrophobic layers. Our approach to hybridize metal nanoparticles in between lipid layers offers a flexible means that allows us to obtain a liposome system with more controllable membrane properties.
Park
Sung-Hee
SH
Amore-Pacific Co. R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-Do, 446-729, South Korea.
Oh
Seong-Geun
SG
Suh
Kyung-Do
KD
Han
Sang-Hoon
SH
Chung
Dong June
DJ
Mun
Ji-Young
JY
Han
Sung-Sik
SS
Kim
Jin-Woong
JW
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D016880:Anisotropy; D001672:Biocompatible Materials / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010758:Phosphorus / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D001672:Biocompatible Materials; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D008563:Membrane Lipids; D015060:1,2-Dipalmitoylphosphatidylcholine; D010758:Phosphorus; D010984:Platinum