title: Technical note: A new and cost-effective method for detection of the bovine acyl-CoA:diacylglycerol acyltransferase 1 K232A polymorphism in cattle.
abstract: A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: R S
authors.initials: RS
authors.lastname: Steinberg
authors.forename: L
authors.initials: L
authors.lastname: Pereira
authors.forename: G A
authors.initials: GA
authors.lastname: Lacorte
authors.forename: M G C D
authors.initials: MG
authors.lastname: Peixoto
authors.forename: R S
authors.initials: RS
authors.lastname: Verneque
authors.forename: R L
authors.initials: RL
authors.lastname: Teodoro
authors.forename: M A
authors.initials: MA
authors.lastname: Machado
authors.forename: C G
authors.initials: CG
authors.lastname: Fonseca
authors.forename: M R S
authors.initials: MR
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D003612:Dairying / Q000379:methods*; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D051048:Diacylglycerol O-Acyltransferase