Restoration of endocrine function and fertility with orthotopic tubal-ovarian allotransplant as the anatomical-functional unit in rabbits.
The aim of this study was to re-establish endocrine and reproductive function in tubal-ovariectomized rabbits using orthotopic tubal-ovary allotransplants (OT-OA). Fifty-five New Zealand White nonconsanguineous rabbits were used and allocated into five experimental groups: Each group comprised donors submitted to right salpingo-oophorectomy that served as the donated allograft. In group 1 no cyclosporin (CsA) was administered and rabbits were submitted to left salpingo-oophorectomy (LSO). Group 2 was the allotransplant group and did not receive CsA. Group 3 was the allotransplant group with CsA. They were submitted to bilateral salpingo-oophorectomy followed by OT-OA. CsA of 10 mg/kg/day was administered to rabbits for the first 21 days and 5 mg/kg/day for the remainder of the study. Group 4 received CsA just as for group 3 and was submitted to LSO. During the study the following were measured: estradiol (E2), progesterone (P4), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and histopathological study of the uteri, tubes, and ovaries. Groups 1, 3, and 4 showed a significant increase in serum E2 and P4 levels corresponding to the second week of gestation, compared to group 2 (p < .05). Group 2 showed a significant increase in serum LH and FSH levels during week 10 compared to serum LH and FSH levels in groups 1, 3, and 4 (p < .05). In group 3, three rabbits did not reject the allotransplant, one rabbit became pregnant, and endocrine function was re-established in two rabbits. It can be concluded that OT-OA together with CsA administration re-establishes endocrine function and fertility.
Meraz
Manuel M
MM
Transplant Unit, Specialties Hospital, 21st Century National Medical Center, IMSS, Mexico, DF, Mexico. mmmeraz@hotmail.com
Juárez
Carmen Gracida
CG
Monsalve
Cristina Revilla
CR
Martínez-Chequer
Juan Carlos
JC
Duvignau
Jesús Macias
JM
Fernández
Enrique M Aburto
EM
Montoya
Salvador
S
Betancour
Eduardo Tena
ET
D000818:Animals; D016572:Cyclosporine / Q000008:administration & dosage; D004958:Estradiol / Q000097:blood; D005187:Fallopian Tubes / Q000473:pathology / Q000502:physiology / Q000637:transplantation*; D005260:Female; D005298:Fertility; D005640:Follicle Stimulating Hormone / Q000097:blood; D013509:Gynecologic Surgical Procedures / Q000379:methods; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage; D007247:Infertility, Female / Q000601:surgery; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000473:pathology / Q000502:physiology / Q000637:transplantation*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011817:Rabbits; D014184:Transplantation, Homologous
D007166:Immunosuppressive Agents; D011374:Progesterone; D004958:Estradiol; D016572:Cyclosporine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone