title: Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM.
abstract: New drug-encapsulating particles were investigated using bright field (BF) scanning transmission electron microscopy (STEM) in a field emission gun (FEG) scanning electron microscope (SEM). Thickness characterization was done based on measuring the effective cross-section for interaction in our sample-detector configuration using calibration particles. A simplified analytical model, taking account of BF-STEM contrast and effective cross-section for interaction, was utilized for transforming projected two-dimensional BF-STEM images into three-dimensional thickness images. The three-dimensional characterization is demonstrated on a new family of biological materials composed of submicron to micron drug-free and drug-encapsulating particles. The importance of using BF-STEM in SEM, relative to other electron microscopy methods, is discussed as well as the lateral and depth resolution.
authors.affiliation: Wolfson Applied Materials Research Center, Tel Aviv University, Israel. barkay@post.tau.ac.il
authors.forename: Zahava
authors.initials: Z
authors.lastname: Barkay
authors.forename: Ilia
authors.initials: I
authors.lastname: Rivkin
authors.forename: Rimona
authors.initials: R
authors.lastname: Margalit
mesh_terms: D002214:Capsules; D004337:Drug Carriers; D021621:Imaging, Three-Dimensional; D017348:Microscopy, Electron, Scanning Transmission / Q000295:instrumentation / Q000379:methods*
chemical_list: D002214:Capsules; D004337:Drug Carriers