[A study on surface plasmon resonance-based gene chip].
The surface plasmon resonance (SPR)-based gene chip was prepared according to the following processes: First, a film of nanogold, which was synthesized by using Frens' method, was plated on chip by Chlorauric acid/hydroxylamine method. Then probes were fixed on nanogold film by Self-assembled monolayer (SAM) technology. Subsequently, the fixing time and concentration of probes, the sensitivity and the specificity of the chip were optimized. Our results suggested that the chip plated with 2.5 nm nanogold film has a better SPR reflection, and when fixed by probes for 4.5 h at the concentration of 1 500 nmol/L, the gene chip also shows a fine performance of detection and can identify accurately the mismatch between bases in SPR detection system. The gene chip constructed in the research can be used for SPR sensor detection.
Gu
Dayong
D
Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Shi
Lei
L
Yu
Huawei
H
Wang
Hua
H
Lu
Weiping
W
Liang
Bing
B
Zhou
Yuanguo
Y
Zhang
Ya'ou
Y
D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009344:Neisseria gonorrhoeae / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D006046:Gold