title: Cutting edge: an IL-17F-CreEYFP reporter mouse allows fate mapping of Th17 cells.
abstract: The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-Cre(EYFP) strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4(+) T cells during experimental autoimmune encephalomyelitis, IL-17F-Cre(EYFP) CD8 T cells robustly expressed IL-17F in response to TGF-beta, IL-6, and IL-23. Fate mapping of IL-17F-expressing reporter T cells revealed a significant down-regulation of Th17 cytokines after homeostatic expansion in RAG1-deficient animals. Despite this loss of effector phenotype, committed Th17 cells were resistant to Foxp3 expression in vitro or in vivo. Thus, the IL-17F-Cre strain furthers our understanding of Th17 biology.
authors.affiliation: First Medical Department, Johannes Gutenberg University of Mainz, Mainz, Germany.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Croxford
authors.forename: Florian C
authors.initials: FC
authors.lastname: Kurschus
authors.forename: Ari
authors.initials: A
authors.lastname: Waisman
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology*; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology; D017930:Genes, Reporter / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D019426:Integrases / Q000235:genetics*; D020381:Interleukin-17 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation
chemical_list: D001426:Bacterial Proteins; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D020381:Interleukin-17; D008164:Luminescent Proteins; D011506:Proteins; D022661:RNA, Untranslated; C054562:yellow fluorescent protein, Bacteria; C045073:Cre recombinase; D019426:Integrases