title: Time-dependent inactivation of human phenylethanolamine N-methyltransferase by 7-isothiocyanatotetrahydroisoquinoline.
abstract: Inhibitors of phenylethanolamine N-methyltransferase [PNMT, the enzyme that catalyzes the final step in the biosynthesis of epinephrine (Epi)] may be of use in determining the role of Epi in the central nervous system. Here we describe the synthesis and characterization of 7-SCN tetrahydroisoquinoline as an affinity label for human PNMT.
authors.affiliation: College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Caine
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Thomson
authors.forename: Meri
authors.initials: M
authors.lastname: Slavica
authors.forename: Gary L
authors.initials: GL
authors.lastname: Grunewald
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McLeish
mesh_terms: D000818:Animals; D004837:Epinephrine / Q000378:metabolism / Q000502:physiology; D006801:Humans; D010625:Phenylethanolamine N-Methyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D044005:Tetrahydroisoquinolines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C538959:7-isothiocyanatotetrahydroisoquinoline; D044005:Tetrahydroisoquinolines; D010625:Phenylethanolamine N-Methyltransferase; D004837:Epinephrine