Phenanthrene uptake by Medicago sativa L. under the influence of an arbuscular mycorrhizal fungus.
Phenanthrene uptake by Medicago sativa L. was investigated under the influence of an arbuscular mycorrhizal fungus. Inoculation of lucerne with the arbuscular mycorrhizal fungus Glomus etunicatum L. resulted in higher phenanthrene accumulation in the roots and lower accumulation in the shoots compared to non-mycorrhizal controls. Studies on sorption and desorption of phenanthrene by roots and characterization of heterogeneity of mycorrhizal and non-mycorrhizal roots using solid-state (13)C nuclear magnetic resonance spectroscopy ((13)C NMR) demonstrated that increased aromatic components due to mycorrhizal inoculation resulted in enhanced phenanthrene uptake by the roots but lower translocation to the shoots. Direct visualization using two-photon excitation microscopy (TPEM) revealed higher phenanthrene accumulation in epidermal cells of roots and lower transport into the root interior and stem in mycorrhizal plants than in non-mycorrhizal controls. These results provide some insight into the mechanisms by which arbuscular mycorrhizal inoculation may influence the uptake of organic contaminants by plants.
Wu
Naiying
N
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, PO Box 2871, Beijing 100085, China.
Huang
Honglin
H
Zhang
Shuzhen
S
Zhu
Yong-Guan
YG
Christie
Peter
P
Zhang
Yong
Y
D001673:Biodegradation, Environmental; D018533:Biomass; D000455:Medicago sativa / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D036641:Microscopy, Fluorescence, Multiphoton; D038821:Mycorrhizae / Q000378:metabolism*; D010616:Phenanthrenes / Q000493:pharmacokinetics*; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000493:pharmacokinetics*; D013559:Symbiosis
D010616:Phenanthrenes; D012989:Soil Pollutants; C031181:phenanthrene