title: Detection of specific bioparticles using antibodies-coated gold nanoparticles and dielectrophoretic impedance measurement.
abstract: In this study, we developed an easy and quick test capable of identifying specific bacteria in one hour. The protocol was established based on the measurement of bacteria quantity on a biochip with comb-like electrodes. Gold nanoparticles coated with anti-Salmonella antibody were used to enhance the dielectrophretic property of Salmonella spp. on the biochip to facilitate the sensitivity.
authors.affiliation: Department of Mechanical Engineering, National Taipei University of Technology, Taipei, Taiwan ROC. jthuang@ntut.edu.tw
authors.forename: Jung-Tang
authors.initials: JT
authors.lastname: Huang
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Yu
authors.forename: Shao-Yi
authors.initials: SY
authors.lastname: Hou
authors.forename: Shiuh-Bin
authors.initials: SB
authors.lastname: Fang
authors.forename: Hung-Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Chu-Zen
authors.initials: CZ
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D000906:Antibodies / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012475:Salmonella / Q000378:metabolism; D013234:Stem Cells; D013997:Time Factors
chemical_list: D000906:Antibodies; D006046:Gold