Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer.
Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.
Paris
Pamela L
PL
Department of Urology, University of California at San Francisco, San Francisco, CA 94115, USA. pparis@cc.ucsf.edu
Kobayashi
Yasuko
Y
Zhao
Qiang
Q
Zeng
Wei
W
Sridharan
Shivaranjani
S
Fan
Tina
T
Adler
Howard L
HL
Yera
Emmanuel R
ER
Zarrabi
M H
MH
Zucker
Stanley
S
Simko
Jeffry
J
Chen
Wen-Tien
WT
Rosenberg
Jonathan
J
D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D009919:Orchiectomy; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
D014408:Biomarkers, Tumor