title: PPARgamma Pro12Ala Pro/Pro and resistin SNP-420 G/G genotypes are synergistically associated with plasma resistin in the Japanese general population.
abstract: OBJECTIVE
  The Ala allele of the Pro12Ala polymorphism (rs1801282) of peroxisome proliferator-activated receptor gamma (PPARgamma) is protective against type 2 diabetes (T2DM). Resistin, secreted from adipocytes, causes insulin resistance in rodents. Resistin gene expression is reduced by the PPARgamma ligand. We previously reported that subjects with the G/G genotype of a resistin gene single nucleotide polymorphism (SNP) at -420 (rs1862513) had the highest circulating resistin levels, followed by C/G and C/C. The aim of this study was to determine the relationship among PPARgamma Pro12Ala polymorphism, resistin SNP-420, and plasma resistin.
  
  
  METHODS
  We cross-sectionally analysed 2077 community-dwelling subjects attending an annual medical check-up. Genotypes were determined by TaqMan analysis. Fasting plasma resistin was measured using ELISA.
  
  
  RESULTS
  Plasma resistin appeared to be higher in subjects with the Pro/Pro genotype of PPARgamma than those with Pro/Ala and Ala/Ala genotypes (mean +/- SE, 11.6 +/- 0.2 vs. 10.4 +/- 0.5 microg/l). Multiple regression analysis, adjusted for age, gender, BMI, and resistin SNP-420, revealed that the Pro/Pro genotype was a positive predictor of plasma resistin (PPARgamma , Pro/Pro vs. Pro/Ala + Ala/Ala, unstandardized regression coefficient (beta) = 1.03, P = 0.0384). The effects of the Pro/Pro genotype of PPARgamma (Pro/Pro vs. Pro/Ala + Ala/Ala) and the G/G genotype of resistin SNP-420 (G/G vs. C/C) on plasma resistin were synergistic (beta = 4.76, P = 0.011).
  
  
  CONCLUSIONS
  The PPARgamma Pro12Ala Pro/Pro and resistin SNP-420 G/G genotypes were synergistically associated with plasma resistin, when adjusted for age, gender, and BMI, in the Japanese general population.
authors.affiliation: Department of Molecular and Genetic Medicine, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. harosawa@m.ehime-u.ac.jp
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Osawa
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Tabara
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Kawamoto
authors.forename: Jun
authors.initials: J
authors.lastname: Ohashi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ochi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Onuma
authors.forename: Wataru
authors.initials: W
authors.lastname: Nishida
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamada
authors.forename: Jun
authors.initials: J
authors.lastname: Nakura
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miki
authors.forename: Hideichi
authors.initials: H
authors.lastname: Makino
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kohara
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D047495:PPAR gamma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D052243:Resistin / Q000097:blood* / Q000235:genetics*
chemical_list: D047495:PPAR gamma; D052243:Resistin