[Effect of Jagged-1 on the cytokine expression of murine myeloid-derived dendritic cells].
OBJECTIVE
To investigate the in vitro effect of soluble Jagged-1/Fc on the cytokine expression of murine myeloid-derived dendritic cells induced by both rmGM-CSF and rmIL-4.


METHODS
The DC culture model under rmGM-CSF plus rmIL-4 conditions was established. The influences of Jagged-1/Fc on the morphology of DC was checked, and the cytokine expression were analyzed by Luminex system and ELISA. The activation capacity of Jagged-1/Fc-induced DC for allogeneic lymphocytes was detected by MTT assay.


RESULTS
Compared with LPS or Zymosan A, the amount of IL-4 expression of Jagged-1/Fc-induced DC increased significantly and the amount of TNF-alpha expression decreased markedly but gamma-secretase inhibitor DAPT reversed the inhibition of Jagged-1/Fc on TNF-alpha expression from DC and returned it to the control level. Jagged-1/Fc altered the amount of IL-10, IL-6, IL-2, IL-12 and IFN-gamma expression unconspicuously and had no effect on TGF-beta expression. The mixed lymphocyte reaction(MLR) revealed that Jagged-1/Fc-induced DC had the weakest ability to activate allogeneic lymphocytes and LPS-induced DC showed the strongest activation ability.


CONCLUSIONS
The results indicate that Jagged-1/Fc-induced DC incline to induce immune tolerance and to prime naïve T cells to the Th2 cells.
Fang
Zhi-yuan
ZY
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, China.
Xing
Fei-yue
FY
Guo
Zhong-feng
ZF
Chen
Ling
L
D000818:Animals; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000166:cytology*; D000072099:Serrate-Jagged Proteins; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D002135:Calcium-Binding Proteins; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D007376:Interleukin-2; D015850:Interleukin-6; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor