Analysis of aliphatic amines using head-column field-enhanced sample stacking in MEKC with LIF detection.
This paper describes the use of head-column field-enhanced sample stacking for on-line sample concentration in MEKC; the method has been applied to the determination of C1-C9 aliphatic amines after their pre-capillary derivatization with 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA). The optimum derivatization, on-line concentration and separation conditions for these aliphatic amines were investigated in detail. The optimized molar ratio of CBQCA to amines was found to be 120:1, and 50 mmol/L borate buffer (pH 9.5) was used for derivatization in order to achieve high efficiency. Nine imidazole aliphatic amines were baseline separated within 12 min by using 50 mmol/L of sodium borate (pH 9.5) and 15 mmol/L of SDS as running buffer. Under the optimum conditions, the detection limits of these nine aliphatic amines ranged from 1.2 to 3.8 pmol/L. S/N=3, which was about 49-173-fold lower than those of conventional sample injections. After validation, the developed method was applied to the determination of C1-C9 aliphatic amines in river water with the average recoveries of 86.7-105%.
Zhang
Jianhua
J
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
Cui
Huimin
H
Xu
Liangjun
L
Zhang
Lan
L
Chen
Guonan
G
D000588:Amines / Q000032:analysis*; D001565:Benzoates / Q000737:chemistry; D001881:Borates / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005453:Fluorescence; D005618:Fresh Water / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis
D000588:Amines; D001565:Benzoates; D001881:Borates; D011804:Quinolines; D014874:Water Pollutants, Chemical; D014867:Water; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde; C010634:sodium borate; D000432:Methanol