Detection of living Salmonella cells using bioluminescence.
ATP-based bioluminescence using mutant firefly luciferase was combined with an immunochromatographic lateral flow test strip assay for Salmonella enteritidis detection. In this combination method, the Salmonella-antibody-gold complex captured at the test line on the test strip was lysed by heat-treatment, and the ATP released from the cells was measured using mutant luciferase. This method resulted in approximately 1,000 times higher sensitivity in the detection of Salmonella (i.e. 10(3) c.f.u./ml) compared to immunochromatographic lateral flow assay.
Urata
Masaaki
M
Research Institute for Nanodevice and Bio Systems, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima, 739-8530, Japan.
Iwata
Rei
R
Noda
Kenichi
K
Murakami
Yuji
Y
Kuroda
Akio
A
D000255:Adenosine Triphosphate / Q000032:analysis; D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D049409:Luciferases, Firefly / Q000378:metabolism; D008163:Luminescent Measurements; D012477:Salmonella enteritidis / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000255:Adenosine Triphosphate; D049409:Luciferases, Firefly