title: Normalized Born ratio for fluorescence optical projection tomography.
abstract: We present a normalized Born approach for fluorescence optical projection tomography that takes into account tissue absorption properties. This approach can be particularly useful to study fluorochrome distribution within tissue. We use the algorithm to three-dimensionally reconstruct and characterize a fluorescein isothiocyanate containing absorptive phantom and an infarcted mouse heart previously injected with a fluorescent molecular probe.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. cvinegoni@mgh.harvard.edu
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
authors.forename: Daniel
authors.initials: D
authors.lastname: Razansky
authors.forename: Jose-Luiz
authors.initials: JL
authors.lastname: Figueiredo
authors.forename: Matthias
authors.initials: M
authors.lastname: Nahrendorf
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D000465:Algorithms; D000818:Animals; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D017202:Myocardial Ischemia / Q000473:pathology*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*