title: ATP measurement predicts porcine islet transplantation outcome in nude mice.
abstract: Current nude mice islet transplantation studies cannot be used prospectively. Therefore, to predict transplantation outcomes, reliable and rapid assays for islet quality assessment are warranted. This study evaluated the predictive power of the porcine islet ATP content on the outcomes of islet transplantation in nude mice. Here, we report that the ATP measurement using a small number of handpicked islets with a diameter of 100 to 150 mum is a good predictor of islet graft efficacy in nude mice. Using receiver-operator characteristic analysis, the area under the curve of the ATP content using a small number of handpicked islets was 0.867 (95% confidence interval 0.744-0.989, P<0.001). The sensitivity and the specificity measured were 83.3% and 73.3%, respectively. In conclusion, a simple and a rapid measurement of intraislet ATP content could be a promising substitute for current nude mice islet transplantation studies.
authors.affiliation: Division of Endocrinology of Metabolism, Department of Internal Medicine, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Jae Hyeon
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Park
authors.forename: Han Na
authors.initials: HN
authors.lastname: Lee
authors.forename: Yeon Yi
authors.initials: YY
authors.lastname: Lee
authors.forename: Ho Sun
authors.initials: HS
authors.lastname: Park
authors.forename: Hyong-Il
authors.initials: HI
authors.lastname: Kim
authors.forename: Jae Eun
authors.initials: JE
authors.lastname: Yu
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Jongwon
authors.initials: J
authors.lastname: Ha
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Kim
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D005947:Glucose / Q000378:metabolism; D006085:Graft Survival; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012372:ROC Curve; D015203:Reproducibility of Results; D013552:Swine; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques; D014183:Transplantation, Heterologous
chemical_list: D015415:Biomarkers; D007328:Insulin; D000255:Adenosine Triphosphate; D013806:Theophylline; D005947:Glucose