title: [Research on optimization system on tissue culture and rapid propagation of Pueraria mirifica].
abstract: OBJECTIVE
  To study the tissue culture and rapid-proliferation techniques of Pueraria mirifica.
  
  
  METHODS
  The tender branch were used as explants and cultivated in different media. The optimum media for inducing buds, proliferation and rooting were selected by adjusting the kinds and doses of plant hormones and special compounds.
  
  
  RESULTS
  The medium of MS + IBA 0.05 mg L(-1) + BA 0.5 mg L(-1) was suitable for buds inducing and could be used in the first generation cultivation; MS + IBA 0. 02 mg L(-1) + BA 0.2 mg L(-1) and MS +BA 0.1 mg L(-1) were employed by turns in subculture, 25 days propagation coefficient was 3.0; and the medium of 1/2MS + IBA 0.1 mg L(-1) + IAA 0.2 mg L(-1) + C (special compound) 10 mg L(-1) was used for roots inducing, the rooting rate was 76.9%. Rooting plantlets were transplanted in spring and summer; the surviving rate was 81.0%.
  
  
  CONCLUSIONS
  This technique system could be employed for rapid propagation of P. mirifica.
authors.affiliation: Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Academia Sinica, Guilin 541006, China.
authors.forename: Ning-Zhen
authors.initials: NZ
authors.lastname: Huang
authors.forename: Feng-Luan
authors.initials: FL
authors.lastname: Tang
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: He
authors.forename: Chuan-Ming
authors.initials: CM
authors.lastname: Fu
authors.forename: Cheng-Xin
authors.initials: CX
authors.lastname: He
authors.forename: Lun-Fan
authors.initials: LF
authors.lastname: Guo
authors.forename: Xin-Gui
authors.initials: XG
authors.lastname: Wang
mesh_terms: D029908:Pueraria / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*