[Evaluation of CD4+ CD25+ regulatory T cells in the peripheral blood of recurrent spontaneous abortion patients].
OBJECTIVE
To explore the role of CD4+ CD25+ regulatory T cells (CD4+ CD25+ Tr) in the pathogenesis of recurrent spontaneous abortion.


METHODS
Peripheral blood samples were collected from 29 women with unexplainable recurrent spontaneous abortion (the URSA group) and another 20 with normal pregnancy (the control group). The percentage of CD4+ CD25+ Tr in the peripheral blood was measured by flow cytometry.


RESULTS
The rate of CD4+ CD25bright Tr in the URSA patients ([1.98 +/- 0.96]%) was significantly lower than that in the control group ([3.21 +/- 1.25]%, P < 0.05), while the percentages of CD4+ CD25+ and CD4+ CD25dim and the ratio of CD4+ CD25bright/CD4+ were not significantly different between the two groups (P > 0.05).


CONCLUSIONS
URSA might be associated with the decreased percentage of CD4+ CD25bright Tr, which plays an important role in fetomaternal immunologic tolerance.
Xia
Xin-Yi
XY
PLA Research Institute of Clinical Laboratory Medicine, School of Clinical Medicine Nanjing University Medical College, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. xiaxy@androl.cn
Yang
Bin
B
Xiong
Ting
T
Lu
Hong-Yong
HY
Ge
Yi-Feng
YF
Yao
Bin
B
Cui
Ying-Xia
YX
Huang
Yu-Feng
YF
D000026:Abortion, Habitual / Q000097:blood / Q000276:immunology*; D000022:Abortion, Spontaneous / Q000097:blood / Q000276:immunology*; D000328:Adult; D015704:CD4 Antigens / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood; D011247:Pregnancy; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit