[RP-hPLC determination of flavonoids in several flowers].
OBJECTIVE
To develvp a RP-HPLC method for the determination of flavonoids in fifteen kinds of flowers such as Iris lacteal pall, prunus persica and rosa chinensis.


METHODS
The contents of quercetin, kaempferol and isorhamntin in fifteen kinds of flowers were extracted with methanol. The analysis was performed on a Kromasil C18 column (4.6 mm x250 mm, 5 microm) with methanol-0.1% phosphoric acid (50:50) as mobile phase.


RESULTS
The quercetin, kaempferol and isorhamntin were separated well, and the result shows that the content of quercetin in the Iris lactea Pall was the highest (1.536%), the contene of kaempferol in Persica persice was the highest (0.572%), and the content of isorhamntin in chrysamthemum morifolium was up to 0.290%.


CONCLUSIONS
The contents of flavonoids in these flowers were by determined RP-HPLC for the first time and the method can be used for quantitative determination of flavonoids in the flowers.
Niu
Ying-Feng
YF
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
Shao
Yun
Y
Zhao
Xiao-Hui
XH
Wen
Huai-Xiu
HX
Tao
Yan-Duo
YD
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D031323:Iris Plant / Q000737:chemistry; D044949:Kaempferols / Q000737:chemistry; D027861:Prunus / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry; D027826:Rosa / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D044949:Kaempferols; C006552:kaempferol; D011794:Quercetin