title: Films of agarose enable rapid formation of giant liposomes in solutions of physiologic ionic strength.
abstract: This paper describes a method to form giant liposomes in solutions of physiologic ionic strength, such as phosphate buffered saline (PBS) or 150 mM KCl. Formation of these cell-sized liposomes proceeded from hybrid films of partially dried agarose and lipids. Hydrating the films of agarose and lipids in aqueous salt solutions resulted in swelling and partial dissolution of the hybrid films and in concomitant rapid formation of giant liposomes in high yield. This method did not require the presence of an electric field or specialized lipids; it generated giant liposomes from pure phosphatidylcholine lipids or from lipid mixtures that contained cholesterol or negatively charged lipids. Hybrid films of agarose and lipids even enabled the formation of giant liposomes in PBS from lipid compositions that are typically problematic for liposome formation, such as pure phosphatidylserine, pure phosphatidylglycerol, and asolectin. This paper discusses biophysical aspects of the formation of giant liposomes from hybrid films of agarose and lipids in comparison to established methods and shows that gentle hydration of hybrid films of agarose and lipids is a simple, rapid, and reproducible procedure to generate giant liposomes of various lipid compositions in solutions of physiologic ionic strength without the need for specialized equipment.
authors.affiliation: Departments of Chemical Engineering, University of Michigan, 1101 Beal Avenue, Ann Arbor, Michigan 48109, USA.
authors.forename: Kim S
authors.initials: KS
authors.lastname: Horger
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Estes
authors.forename: Ricardo
authors.initials: R
authors.lastname: Capone
authors.forename: Michael
authors.initials: M
authors.lastname: Mayer
mesh_terms: D055592:Biophysical Phenomena; D002021:Buffers; D002784:Cholesterol / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D009994:Osmolar Concentration; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D002021:Buffers; D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D012965:Sodium Chloride; C024990:asolectin; C042511:1,2-dioleoylphosphatidylserine; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; D012685:Sepharose; D002784:Cholesterol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine