[Effect of supplement energy and nourish lung herbs on drug-resistance lung cancer with mechanisms of mitochondrial and caspase signal].
OBJECTIVE
To explore the effect of supplement energy and nourish lung (SENL) herbs on the growth of drug-resistance lung cancer cells, expression of multidrug resistance-associated protein (MRP) and mechanisms of mitochondrial and Caspase signal.


METHODS
Drug-resistance lung cancer cell line SW1573/2R120 was treated with SENL herbs and Adriamycin. Cellular toxicity with MTT colorimetric assay, expression of MRP protein with flow microscopy, the fluorescence intensity of mitochondria membrane potetional and Ca2+ with laser confocal microscopy, the activities of caspase-3 and caspase-8 with colorimetric assay were detected.


RESULTS
Compared with control group, cellular inhibitory rate was increased obviously, expression of MRP protein was decreased, mitochondria membrane potetional and intracellular Ca2+ were decreased, the activities of caspase-3 and caspase-8 were increased in the group of SENL herbs.


CONCLUSIONS
SENL herbs can interfere and reverse drug resistance in lung cancer by the mechanism of decreasing mitochondria membrane potetional and increasing the activities of caspase-3 and caspase-8.
Xu
Meng
M
Department of Oncology, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China. xumengdoctor@yahoo.com
Zhu
Xin-Hai
XH
Sheng
Liang-He
LH
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D004338:Drug Combinations; D019008:Drug Resistance, Neoplasm; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry
D000970:Antineoplastic Agents; D002118:Calcium; D053148:Caspase 3; D053181:Caspase 8; D004317:Doxorubicin; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D027425:ATP-Binding Cassette, Sub-Family C Proteins