title: Enhanced bone formation by marrow-derived endothelial and osteogenic cell transplantation.
abstract: Bone marrow-derived osteogenic cells can regenerate bone tissuesin vivo. The aim of the present study is to determine whether the cotransplantation of bone marrow-derived endothelial-like cells (BMECs) enhances bone regeneration by bone marrow-derived osteogenic cell (BMOC) transplantation in osseous defects. Canine bone marrow cells were differentiated separately into BMECs and BMOCs. Using apatite-coated poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds as cell delivery vehicles, BMOCs were transplanted with or without BMECs into critical-sized calvarial defects in immunodeficient mice. Histological analyses, microcomputed tomography, and soft X-ray were performed to assess mineralized bone formation at 8 weeks. Cotransplantation of BMECs and BMOCs resulted in greater bone formation than transplantation of BMOCs alone. There was a significant (p < 0.05) increase in bone formation area following cotransplantation (30.8% +/- 2.5%), compared with transplantation of BMOCs alone (15.3% +/- 1.9%). These results demonstrate that the cotransplantation of BMECs enhances bone regeneration mediated by BMOC transplantation in osseous defects.
authors.affiliation: Department of Bioengineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Park
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Sun Woong
authors.initials: SW
authors.lastname: Kang
authors.forename: Kang-Min
authors.initials: KM
authors.lastname: Ahn
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000000981:diagnostic imaging; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology / Q000000981:diagnostic imaging / Q000637:transplantation*; D006801:Humans; D007150:Immunohistochemistry; D010012:Osteogenesis / Q000502:physiology*; D055114:X-Ray Microtomography