Endometrial expression of selected transcripts involved in prostaglandin synthesis in cows with endometritis.
Several cytokines and prostaglandins play an important role in preparing the endometrium for implantation and mediating pro-inflammatory events. The aim of the present study was to examine mRNA expression of interleukin 1alpha (IL-1alpha), interleukin receptor antagonist (IL-1-RN), cytosolic prostaglandin E synthase (cPGES), microsomal PGES (mPGES-1 and mPGES-2) and lipocalin-type PGDS (L-PGDS) in the bovine endometrium. Endometrial epithelium samples were collected ex vivo from cows with different status of health at day 21-27 postpartum on a dairy farm. Three groups (n=9 animals each) were defined: (1) healthy cows with no signs of endometritis (control group), (2) cows with subclinical endometritis, and (3) cows with signs of clinical endometritis. Oestrous cycle-dependent mRNA expression pattern was investigated using bovine endometrial epithelial cells from healthy uteri collected at the abattoir. These uteri were classified into post-ovulatory, early-to-mid luteal, late luteal or pre-ovulatory phase (n=8 animals for each cycle phase). After collecting endometrial epithelium using the cytobrush-method, mRNA analysis was performed by real-time RT-PCR. L-PGDS, IL-1alpha and IL-1-RN mRNA were expressed significantly higher (P<0.05) in the endometrium of cows with subclinical or clinical endometritis compared with healthy cows. A twofold lower cPGES mRNA expression (P<0.05) was detected in cows with subclinical endometritis compared to healthy cows. L-PGDS and IL-1-RN mRNA expression was increased (P<0.05) after ovulation compared with the pre-ovulatory or luteal phase, respectively. These results support the hypothesis that a dys-regulated cytokine and/or prostaglandin profile in the uterus could be induced by subclinical endometritis or clinical endometritis.
Gabler
C
C
Institute of Veterinary-Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany. gablerch@zedat.fu-berlin.de
Drillich
M
M
Fischer
C
C
Holder
C
C
Heuwieser
W
W
Einspanier
R
R
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D004716:Endometritis / Q000378:metabolism / Q000662:veterinary*; D004717:Endometrium / Q000737:chemistry*; D004848:Epithelium / Q000737:chemistry; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D015870:Gene Expression; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D053582:Interleukin-1alpha / Q000235:genetics; D019746:Intramolecular Oxidoreductases / Q000235:genetics; D054834:Lipocalins / Q000235:genetics; D008861:Microsomes / Q000201:enzymology; D049590:Postpartum Period / Q000378:metabolism; D000071876:Prostaglandin-E Synthases; D011453:Prostaglandins / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D053590:Interleukin 1 Receptor Antagonist Protein; D053582:Interleukin-1alpha; D054834:Lipocalins; D011453:Prostaglandins; D012333:RNA, Messenger; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase; D000071876:Prostaglandin-E Synthases