title: Applications of optical resonance to biological imaging and label-free protein microarrays.
abstract: We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for protein binding in a high-throughput micro-array format.
authors.affiliation: Departments of Electrical and Computer Engineering, Physics and Biomedical Engineering at Boston University, MA 02215, USA. selim@bu.edu
authors.forename: M Selim
authors.initials: MS
authors.lastname: Unlü
authors.forename: I E
authors.initials: IE
authors.lastname: Ozkumur
authors.forename: J
authors.initials: J
authors.lastname: Needham
authors.forename: D A
authors.initials: DA
authors.lastname: Bergstein
authors.forename: B B
authors.initials: BB
authors.lastname: Goldberg
authors.forename: A
authors.initials: A
authors.lastname: Yalcin
authors.forename: P
authors.initials: P
authors.lastname: Spuhler
authors.forename: R
authors.initials: R
authors.lastname: Irani
authors.forename: C
authors.initials: C
authors.lastname: DeLisi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D009693:Nucleic Acid Hybridization; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins