An electrochemiluminescent biosensor for glucose based on the electrochemiluminescence of luminol on the nafion/glucose oxidase/poly(nickel(II)tetrasulfophthalocyanine)/multi-walled carbon nanotubes modified electrode.
A poly(nickel(II) tetrasulfophthalocyanine)/multi-walled carbon nanotubes composite modified electrode (polyNiTSPc/MWNTs) was fabricated by electropolymerization of NiTSPc on MWNTs-modified glassy carbon electrode (GCE). The modified electrode was found to be able to greatly improve the emission of luminol electrochemiluminescence (ECL) in a solution containing hydrogen peroxide. Glucose oxidase (GOD) was immobilized on the surface of polyNiTSPc/MWNTs modified GC electrode by Nafion to establish an ECL glucose sensor. Under the optimum conditions, the linear response range of glucose was 1.0x10(-6) to 1.0x10(-4) mol L(-1) with a detection limit of 8.0x10(-8) mol L(-1) (defined as the concentration that could be detected at the signal-to-noise ratio of 3). The ECL sensor showed an outstanding well reproducibility and long-term stability. The established method has been applied to determine the glucose concentrations in real serum samples with satisfactory results.
Qiu
Bin
B
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fuzhou, Fujian 350002, China.
Lin
Zhenyu
Z
Wang
Jian
J
Chen
Zhihuang
Z
Chen
Jinhua
J
Chen
Guonan
G
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D008163:Luminescent Measurements; D008165:Luminol / Q000032:analysis; D037742:Nanotubes, Carbon / Q000737:chemistry
D037742:Nanotubes, Carbon; D008165:Luminol; D005949:Glucose Oxidase; D005947:Glucose