title: Cationic nucleoside lipids based on a 3-nitropyrrole universal base for siRNA delivery.
abstract: Cationic nucleoside lipids based on a 3-nitropyrrole universal base were prepared from D-ribose using a straightforward chemical synthesis. Several studies including DLS, TEM, and ethidium bromide (EthBr) assay demonstrated that these amphiphilic molecules form supramolecular organizations of nanometer size in aqueous solutions and are able to bind nucleic acids. siRNA knockdown experiments were performed with these nucleolipids, and we observed protein knockdown activity similar to the siPORT NeoFX positive control. No significant cytotoxicity was found.
authors.affiliation: Centre Interdisciplinaire de Nanosciences de Marseille CINaM, UPR-CNRS 3118, Université Aix-Marseille II, Luminy, Case 913, 13288 Marseille Cedex 09, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Ceballos
authors.forename: Carla A H
authors.initials: CA
authors.lastname: Prata
authors.forename: Suzanne
authors.initials: S
authors.lastname: Giorgio
authors.forename: Frédéric
authors.initials: F
authors.lastname: Garzino
authors.forename: Dominique
authors.initials: D
authors.lastname: Payet
authors.forename: Philippe
authors.initials: P
authors.lastname: Barthélémy
authors.forename: Mark W
authors.initials: MW
authors.lastname: Grinstaff
authors.forename: Michel
authors.initials: M
authors.lastname: Camplo
mesh_terms: D000818:Animals; D002460:Cell Line; D055785:Gene Knockdown Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D034741:RNA, Small Interfering / Q000378:metabolism*; D012263:Ribonucleosides / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D014162:Transfection / Q000379:methods*
chemical_list: C462529:1-ribofuranosyl-3-nitropyrrole; D008055:Lipids; D011758:Pyrroles; D034741:RNA, Small Interfering; D012263:Ribonucleosides