A rhodamine-hydroxamic acid-based fluorescent probe for hypochlorous acid and its applications to biological imagings.
A new rhodamine-hydroxamic acid-based fluorescent chemosensor for the rapid detection of HOCl in aqueous media was developed. The system, which utilizes an irreversible HOCl-promoted oxidation reaction, responds instantaneously at room temperature with linear proportionality to the amount of HOCl. This system is highly selective for HOCl over other reactive oxygen species (ROS) and highly sensitive in aqueous solutions. Biological imaging studies using living cells and organisms (A549 cells and zebrafish) to detect HOCl are successfully demonstrated.
Yang
Young-Keun
YK
Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Cho
Hyungseoph Jason
HJ
Lee
Jihyun
J
Shin
Injae
I
Tae
Jinsung
J
D000818:Animals; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D006877:Hydroxamic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D006997:Hypochlorous Acid / Q000737:chemistry*; D015394:Molecular Structure; D017382:Reactive Oxygen Species / Q000737:chemistry; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry; D015027:Zebrafish
D005456:Fluorescent Dyes; D006877:Hydroxamic Acids; D017382:Reactive Oxygen Species; D012235:Rhodamines; D006997:Hypochlorous Acid