[Laboratory medicine in the post-genome era: experiences in Chiba University Hospital].
Since the completion of the human genome project, there is growing interest in the clinical application of genome sciences. For this purpose, particular attention toward identifying at-risk individuals and understanding the complexities of the testing process are essential. In this article, I describe the importance of clinical genetics and genetic counseling, and explain how and why the division of laboratory medicine is involved in these tasks in Chiba University Hospital. Our genetic counseling team consists of a clinical laboratory physician qualified as a clinical geneticist, medical technologist qualified as a genetic counselor, clinical psychologists, and a medical social worker. We treat more than 100 cases including late-onset, incurable neurological diseases, hereditary tumors, prenatal diagnosis, and chromosomal abnormalities. The sequencing of the human genome has paved the way for comprehensive transcriptome and proteome analyses. Since the detailed understanding of biological processes, both in healthy and pathological states, requires the direct study of relevant proteins, proteomics bridges the gap between the information coded in the genome sequence and cellular behavior. Therefore, proteomics is among the most promising technologies for the development of novel diagnostic tools. Recent advances in sophisticated technologies in proteomics should identify promising ways to discover novel markers in various fields of clinical medicine. In this presentation, I will give a definition of the proteome, and outline the basic methodologies for proteome analyses. I will also present our experiences in identifying novel biomarker candidates in hepatobiliary diseases, and discuss future perspectives of clinical proteomics in laboratory medicine.
Nomura
Fumio
F
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Division of Laboratory Medicine and Clinical Genetics, Chiba University Hospital, Chiba 260-8670, Japan. fnomura@faculty.chiba-u.jp
D004501:Education, Medical; D015180:Electrophoresis, Gel, Two-Dimensional; D005817:Genetic Counseling; D005823:Genetics / Q000193:education; D006785:Hospitals, University; D016045:Human Genome Project; D006801:Humans; D007564:Japan; D007755:Laboratories, Hospital / Q000639:trends*; D040081:Protein Array Analysis; D020543:Proteome; D040901:Proteomics / Q000639:trends*
D020543:Proteome