[HPLC fingerprint analysis of cordyceps and mycelium of cultured cordy].
OBJECTIVE
To establish HPLC fingerprints of cordyceps and mycelium of cultured cordy for quality control.


METHODS
The analysis was carried out on a Diamonsil C18 column eluted with phosphate buffer (pH 6.5) and methanol as mobile phase, the gradient elution program was used, and the detection wavelength was 260 nm; The similarity of fingerprints was evaluated, then, cluster analysis was studied.


RESULTS
The method of HPLC fingerprint analysis was validated and in keeping with the requirement. Ten common peaks were found in HPLC fingerprint. Cordyceps from different areas and mycelium of cultured cordy could be distinguished by the HPLC fingerprint.


CONCLUSIONS
All results above exhibit that this method is practicable, reproducible, and reliable as a method for the quality control of cordyceps and mycelium of cultured cordy at present.
Wu
Yan-Shu
YS
PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, China.
Zhou
Dan-Lei
DL
Yan
Dan
D
Ren
Yong-Shen
YS
Fang
Yi-Lin
YL
Xiao
Xiao-He
XH
Du
Xiao-Xi
XX
Wang
Jian
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry*; D025282:Mycelium / Q000737:chemistry*; D015203:Reproducibility of Results