title: Construction of a BAC library for buckwheat genome research - an application to positional cloning of agriculturally valuable traits.
abstract: We have constructed a BAC library for common buckwheat Fagopyrum esculentum Moench. The library includes 142,005 clones with an average insert size of approximately 76 kb, equivalent to approximately a 7 to approximately 8-fold coverage of the genome. Polymerase chain reaction based screening of the library with AGAMOUS and FLORICAULA/LEAFY primers, has identified 7 and 9 BACs, respectively, which are consistent with the genome coverage. This library represents the first large insert genomic library for F. esculentum and it can be served as a genetic resource facilitating agricultural, pharmacological, physiological, and evolutionary studies of the species. To demonstrate the utilization of the library for characterizing agriculturally valuable traits, we developed a sequence tagged site marker tightly linked to the dwarf E locus as well as to the self-incompatibility complex locus and screened the library to initiate positional cloning of the causative genes.
authors.affiliation: Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yasui
authors.forename: Masashi
authors.initials: M
authors.lastname: Mori
authors.forename: Daiki
authors.initials: D
authors.lastname: Matsumoto
authors.forename: Ohmi
authors.initials: O
authors.lastname: Ohnishi
authors.forename: Clayton G
authors.initials: CG
authors.lastname: Campbell
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ota
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis; D019612:Fagopyrum / Q000235:genetics*; D015723:Gene Library; D023061:Gene Order; D008040:Genetic Linkage; D018745:Genome, Plant / Q000235:genetics*
chemical_list: D018744:DNA, Plant