Photodynamic effect of hypericin in primary cultures of human umbilical endothelial cells and glioma cell lines.
Hypericin is the most powerful naturally occurring photosensitizer and as such there is renaissant interest in the potentials of this compound for anticancer photodynamic therapy (PDT). The purpose of this study was to investigate the hypericin-mediated photodynamic therapy effects on normal human umbilical endothelial cells (HUVECs) in comparison with cancer human glioma cell lines U-87 MG and U-373 MG, in in vitro conditions. The data suggest that endothelial cells as well as glioma cell lines are sensitive only to photoactivated hypericin. The inhibitory effects of photoactivated hypericin did not differ in endothelial compared with tumor cells in cytotoxicity MTT and DNA fragmentation assays. However, an important difference in sensitivity was found between the above mentioned cell types in migration and metalloproteinases inhibition assays performed as cell function tests. The findings in both function tests were supported by the high sensitivity of endothelial cells in an additional angiogenesis test of tubular formation in vitro.
Stupáková
Viktória
V
Department of Pharmacology, Faculty of Medicine, University of P. J. Safárik, Kosice, Slovak Republic.
Varinská
Lenka
L
Mirossay
Andrej
A
Sarisský
Marek
M
Mojzis
Ján
J
Dankovcík
Róbert
R
Urdzík
Peter
P
Ostró
Alexander
A
Mirossay
Ladislav
L
D000873:Anthracenes; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D053938:DNA Fragmentation; D042783:Endothelial Cells / Q000187:drug effects*; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*
D000873:Anthracenes; D017319:Photosensitizing Agents; D010569:Perylene; C004965:hypericin; D018093:Gelatinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9