title: N-glycosylation of plant recombinant pharmaceuticals.
abstract: N-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is N-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry, N-glycan profile analysis, and glycopeptide identification by LC-MS.
authors.affiliation: Faculté des Sciences, Université de Rouen, Mont Saint Aignan, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Bardor
authors.forename: Gleysin
authors.initials: G
authors.lastname: Cabrera
authors.forename: Johannes
authors.initials: J
authors.lastname: Stadlmann
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.forename: José A
authors.initials: JA
authors.lastname: Cremata
authors.forename: Véronique
authors.initials: V
authors.lastname: Gomord
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Fitchette
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D006801:Humans; D004364:Pharmaceutical Preparations; D010944:Plants / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004364:Pharmaceutical Preparations; D011994:Recombinant Proteins