Application of Click-chemistry-based perphenylcarbamated beta-CD chiral stationary phase in CEC.
A novel chiral stationary phase (CSP) was prepared by anchoring mono-6-azido-6-deoxyperphenylcarbamated beta-CD onto omega-alkynyl functionalized silica (5 microm) via organic soluble Cu(I)-catalyzed Click chemistry. The obtained CSP was thereafter packed into fused-silica capillary (100 microm id) with an effective length of 9 cm and tested in aqueous CEC to separate a series of racemic aryl alcohols. High separation factors with good resolution were achieved using the current novel CSP. Some pharmaceutical compounds could also be well resolved on this newly derived CSP. The analytical results demonstrate that Click-chemistry-based perphenylcarbamated CSP affords high stability in high electric field and depicts excellent enantioseparation in CEC. The effects of pH value, buffer concentration, applied voltage, concentration of organic modifier and analyte structure on the enantioseparation are also discussed.
Wang
Yong
Y
School of Chemical & Biomedical Engineering, Nanyang Technological University, Singapore, Singapore.
Xiao
Yin
Y
Tan
Timothy Thatt Yang
TT
Ng
Siu-Choon
SC
D001592:Benzyl Alcohols / Q000737:chemistry / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D048448:Phenylcarbamates / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D001592:Benzyl Alcohols; D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide