[Fractal research of neurite growth in immunofluorescent images].
Fractal dimension has been widely used in medical images processing and analysis. The neurite growth of cultured dorsal root ganglion (DRG) was detected by fluorescent immunocytochemistry treated with nerve regeneration factor (0.1, 0.5, 2.0 mg/L). A novel method based on triangular prism surface area (TPSA) was introduced and adopted to calculate the fractal dimension of the two-dimensional immunofluorescent images. Experimental results demonstrate that this method is easy to understand and convenient to operate, and the quantititve results are concordant with the observational findings under microscope. This method can be guidelines for analyzing and deciding experimental results.
Tang
Min
M
College of Automation Engineering, Nanjing University of Aeronautics & Astronautic, Nanjing 210016, China. tangmnt@yahoo.com.cn
Wang
Huinan
H
D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000379:methods*; D017709:Fractals; D005727:Ganglia, Spinal / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000502:physiology*
D009414:Nerve Growth Factors