Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells.
Exposure to high levels of ozone (O(3)) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O(3)-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O(3)-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O(3) and/or O(3)-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O(3) and/or its cytotoxic products and may provide protections against O(3)-induced biotoxicities.
Castagna
R
R
Dipartimento di Scienze e Tecnologie Chimiche, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
Davis
P A
PA
Vasu
V T
VT
Soucek
K
K
Cross
C E
CE
Greci
L
L
Valacchi
G
G
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D005609:Free Radicals; D016209:Interleukin-8; D010083:Oxidants, Photochemical; D012333:RNA, Messenger; D010126:Ozone; C003959:TEMPO