title: Mechanical property analysis of stored red blood cell using optical tweezers.
abstract: The deformation of human red blood cells subjected to direct stretching by optical tweezers was analyzed. The maximum force exerted by optical tweezers on the cell via a polystyrene microbead 5microm in diameter was 315pN. Digital image correlation (DIC) method was introduced to calculate the force and the deformation of the cell for the first time. Force-extension relation curves of the biconcave cell were quantitatively assessed when erythrocytes were stored in Alsever's Solution for 2 days, 5 days, 7 days and 14 days respectively. Experiment results demonstrated that the deformability of red blood cells was impaired with the stored time.
authors.affiliation: School of Aerospace, FML, Tsinghua University, Beijing, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.forename: Cheng
authors.initials: C
authors.lastname: Wen
authors.forename: Huimin
authors.initials: H
authors.lastname: Xie
authors.forename: Anpei
authors.initials: A
authors.lastname: Ye
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yin
mesh_terms: D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D003198:Computer Simulation; D004867:Equipment Design; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D052898:Optical Tweezers; D011137:Polystyrenes / Q000737:chemistry; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D011137:Polystyrenes