Contemporary methacrylate resin-based root canal sealers exhibit different degrees of ex vivo cytotoxicity when cured in their self-cured mode.
The cytotoxicity of four methacrylate resin-based sealers was investigated by the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay, which measures cell viability by assessing its succinate dehydrogenase activity. The sealers were polymerized in the self-cured mode to simulate the setting condition upon their extrusion into periradicular tissues. Disks were prepared from EndoREZ (Ultradent, South Jordan, UT), RealSeal (SybronEndo, Orange, CA), MetaSEAL (Parkell, Farmington, NY), and RealSeal SE (SybronEndo) together with positive and negative controls. After setting, they were placed in direct contact with rat osteosarcoma (ROS 17/2.8) cells and for 5 succeeding weeks after immersing in simulated body fluid (SBF). All sealers exhibited severe toxicity initially (week 0). EndoREZ and RealSeal remained severely toxic after five cycles of SBF immersion. Toxicity of the two self-etching resin-based sealers MetaSEAL and RealSeal SE decreased gradually over time. Transmission electron microscopy of cells exposed to RealSeal SE showed variable degrees of cell injury that reflect its toxicity status. Cells with intact mitochondria were identifiable after the sealer became noncytotoxic at week 5.
Ames
Jason M
JM
Department of Endodontics, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1129, USA.
Loushine
Robert J
RJ
Babb
Brian R
BR
Bryan
Thomas E
TE
Lockwood
Petra E
PE
Sui
Mai
M
Roberts
Steven
S
Weller
R Norman
RN
Pashley
David H
DH
Tay
Franklin R
FR
D000180:Acrylic Resins / Q000633:toxicity*; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003188:Composite Resins / Q000633:toxicity; D008422:Materials Testing; D008689:Methacrylates / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D010006:Osteoblasts / Q000187:drug effects*; D051381:Rats; D012387:Root Canal Filling Materials / Q000633:toxicity*; D055116:Self-Curing of Dental Resins
D000180:Acrylic Resins; D003188:Composite Resins; C481259:EndoRez; D008689:Methacrylates; C511498:Real Seal composite resin; D012387:Root Canal Filling Materials; C534239:metaseal