[Identification of glucose-responsive elements in the promoter of UDP-L-rhamnose biosynthesis gene RHM1 in Arabidopsis thaliana].
In plants, UDP-L-rhamnose is one of the major components of cell wall skeleton. Rhamnose synthase plays a key role in rhamnose synthesis which converts UDP-D-glucose into UDP-L-rhamnose in plants. In this study, we isolated the 1058 bp promoter region of the rhamnose synthase gene AtRHM1 from Arabidopsis genome by PCR, and created a series of deletions of AtRHM1 promoter ranging from -931 bp to +127 bp. The full length of the promoter and its deletion derivatives fused with GUS reporter gene were introduced into wild-type Arabidopsis by Agrobacterium-mediated transformation respectively. The GUS staining and GUS enzymatic activity assay showed that the expression of AtRHM1 is induced at transcriptional level by glucose and the regulatory elements involved in the glucose response are located in the region of -931 bp - -752 bp which contains three G-box motifs.
Ji
Qinmei
Q
School of Life Sciences, Soochow University, Suzhou 215006, China.
Wang
Jinfeng
J
Jiang
Ling
L
Shen
Songdong
S
Fan
Yunliu
Y
Zhang
Chunyi
C
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D014532:Uridine Diphosphate Glucose / Q000235:genetics / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D014539:Uridine Diphosphate Sugars; C022183:UDP-rhamnose; D005964:Glucosyltransferases; C519657:RHM1 protein, Arabidopsis; D014532:Uridine Diphosphate Glucose