Applications of optical resonance to biological imaging and label-free protein microarrays.
We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for protein binding in a high-throughput micro-array format.
Unlü
M Selim
MS
Departments of Electrical and Computer Engineering, Physics and Biomedical Engineering at Boston University, MA 02215, USA. selim@bu.edu
Ozkumur
I E
IE
Needham
J
J
Bergstein
D A
DA
Goldberg
B B
BB
Yalcin
A
A
Spuhler
P
P
Irani
R
R
DeLisi
C
C
D000818:Animals; D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D009693:Nucleic Acid Hybridization; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D005456:Fluorescent Dyes; D011506:Proteins