title: Influence of preoperative feeding on the healing of colonic anastomoses in malnourished rats.
abstract: BACKGROUND
  Malnutrition influences healing of gastrointestinal anastomoses. The authors hypothesize that colonic anastomotic healing is decreased by malnutrition and might be improved by preoperative feeding.
  
  
  METHODS
  Eighty adult male Wistar rats were divided into 4 groups: (1) control rats 1 (C1), fed regular chow ad libitum for 21 days; (2) malnourished pair-fed rats (M), fed 50% of the food ingested by the control rats for 21 days; (3) preoperative nutrition rats (PRE), fed 50% of the average of the controls for 21 days and then fed preoperative nutrition with regular chow ad libitum for 1 week before the operation; and (4) control rats 2 (C2), fed regular chow ad libitum for 28 days. On days 21 (C1 and M) and 28 (PRE and C2), rats underwent 2 colonic transections and, subsequently, 2 end-to-end anastomoses. Rats were killed on postoperative day 5. The anastomoses were resected for tensile strength and histological analysis.
  
  
  RESULTS
  PRE rats showed increased maximal tensile strength vs the M group (0.09+/-0.01 vs 0.15+/-0.01; P<.05) and similar values of maximal tensile strength as the controls (0.15+/-0.01 vs 0.15+/-0.02; P=.91). Collagen type I was higher in controls vs the PRE group (6.13+/-0.39 vs 4.90+/-1.53; P<.05); nevertheless, the PRE group showed higher collagen type I than M rats (4.90+/-0.36 vs 3.83+/-0.35; P<.05).
  
  
  CONCLUSIONS
  Preoperative feeding for 7 days increases the maximal tensile strength, as well as the percentage area of mature collagen, approaching similar values as the control group.
authors.affiliation: Department of Surgery, Hospital das Clinicas, Federal University of Paraná, Curitiba, PR, Brazil. carolgg@gmail.com
authors.forename: Carolina Gomes
authors.initials: CG
authors.lastname: Gonçalves
authors.forename: Anne Karoline
authors.initials: AK
authors.lastname: Groth
authors.forename: Marcelo
authors.initials: M
authors.lastname: Ferreira
authors.forename: Jorge Eduardo F
authors.initials: JE
authors.lastname: Matias
authors.forename: Júlio Cezar U
authors.initials: JC
authors.lastname: Coelho
authors.forename: Antonio Carlos L
authors.initials: AC
authors.lastname: Campos
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001835:Body Weight; D003094:Collagen / Q000032:analysis; D003106:Colon / Q000601:surgery*; D005502:Food; D008297:Male; D044342:Malnutrition; D011300:Preoperative Care / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000032:analysis; D013718:Tensile Strength / Q000502:physiology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D012709:Serum Albumin; D003094:Collagen