Embryo quality and implantation rate in two different culture media: ISM1 versus Universal IVF Medium.
OBJECTIVE
To compare the outcome of two different culture media marketed by the MediCult AS Company (Jyllinge, Denmark)-Universal IVF Medium and ISM1 Medium culture-which, in addition to glucose, pyruvate, and energy-providing components, also contain amino acids, nucleotides, vitamins, and cholesterol.


METHODS
Laboratory and retrospective clinical study.


METHODS
University teaching hospital.


METHODS
A total of 726 patients, undergoing IVF-intracytoplasmic sperm injection procedure, comparable in mean age range, oocyte retrieval, and infertility indication, were included in the study. Laboratory quality and standard procedures were maintained unaffected.


METHODS
Oocyte retrieval, different embryo culture media.


METHODS
Embryo quality, ongoing pregnancy, and implantation rate.


RESULTS
The frequency of good-quality embryos (79% vs. 74%) and the percentages of ongoing pregnancy (27.5% vs. 18%) and implantation rate (15% vs. 10%) were significantly higher in the group treated with ISM1 Medium rather than Universal IVF Medium.


CONCLUSIONS
ISM1 Medium culture seems to improve the performance of embryonic growth and development, as well as increasing the percentage of pregnancy.
Xella
Susanna
S
Mother-Infant Department, Institute of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Modena, Italy. susannaxella@yahoo.it
Marsella
Tiziana
T
Tagliasacchi
Daniela
D
Giulini
Simone
S
La Marca
Antonio
A
Tirelli
Alessandra
A
Volpe
Annibale
A
D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002469:Cell Separation; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D018873:Pregnancy Rate; D011786:Quality Control; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology; D055815:Young Adult
D003470:Culture Media