Proteomic and genomic analysis of PITX2 interacting and regulating networks.
Pituitary homeobox 2 (PITX2) is a homeodomain transcription factor that has a substantial role in cell proliferation and differentiation in various tissues. In this report, we have conducted a systematic study, using proteomic and genomic approaches, to characterize PITX2-interacting proteins and PITX2-regulating genes. We identified four novel PITX2-associated protein partners Y box binding factor-1, heterogeneous ribonucleoprotein K, nucleolin and heterogeneous nuclear ribonucleoprotein U in mass spectrometry analysis. We also found that overexpression of PITX2 upregulated 868 genes (2-25-fold) and downregulated 191 genes (2-15-fold) in DNA microarray analysis. These data provide an insightful perspective for further studying PITX2 function and mechanism of action.
Huang
Yue
Y
Biomedical Science Graduate Program, Marshall University, Huntington, WV 25755, USA.
Huang
Kan
K
Boskovic
Goran
G
Dementieva
Yulia
Y
Denvir
James
J
Primerano
Donald A
DA
Zhu
Guo-Zhang
GZ
D001665:Binding Sites; D002460:Cell Line; D005786:Gene Expression Regulation; D023281:Genomics; D018398:Homeodomain Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology; D011485:Protein Binding; D040901:Proteomics; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000097577:Homeobox Protein PITX2
D018398:Homeodomain Proteins; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2