Rapid method for multi-residue determination of avermectins in bovine liver using high-performance liquid chromatography with fluorescence detection.
A simple method using high-performance liquid chromatography and fluorescence detection is presented for the simultaneous determination of the antiparasitic agents avermectins (abamectin, ivermectin and doramectin) in bovine liver. Samples were extracted using acetonitrile and cleaned up on an alumina SPE column and derivatized with trifluoroacetic anhydride. The derivatives were detected using fluorescence detector set at 365 nm excitation and 475 nm emission wavelengths. As the results, the limit of quantification for three avermectins was about 1 microg/kg. Inter-assay showed the mean recoveries of avermectins in bovine liver ranged from 72% to 81%, with RSD less than 9.9%.
Wang
Hai
H
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, 18 Shuangqing Road, 100085 Haidian District, Beijing, People's Republic of China.
Wang
Zijian
Z
Liu
SuYing
S
Liu
Ziru
Z
D000818:Animals; D000977:Antiparasitic Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000977:Antiparasitic Agents; D007559:Ivermectin; C019264:avermectin; C084101:doramectin