ATP measurement predicts porcine islet transplantation outcome in nude mice.
Current nude mice islet transplantation studies cannot be used prospectively. Therefore, to predict transplantation outcomes, reliable and rapid assays for islet quality assessment are warranted. This study evaluated the predictive power of the porcine islet ATP content on the outcomes of islet transplantation in nude mice. Here, we report that the ATP measurement using a small number of handpicked islets with a diameter of 100 to 150 mum is a good predictor of islet graft efficacy in nude mice. Using receiver-operator characteristic analysis, the area under the curve of the ATP content using a small number of handpicked islets was 0.867 (95% confidence interval 0.744-0.989, P<0.001). The sensitivity and the specificity measured were 83.3% and 73.3%, respectively. In conclusion, a simple and a rapid measurement of intraislet ATP content could be a promising substitute for current nude mice islet transplantation studies.
Kim
Jae Hyeon
JH
Division of Endocrinology of Metabolism, Department of Internal Medicine, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Park
Sang Gyu
SG
Lee
Han Na
HN
Lee
Yeon Yi
YY
Park
Ho Sun
HS
Kim
Hyong-Il
HI
Yu
Jae Eun
JE
Kim
Sun Hee
SH
Park
Chung-Gyu
CG
Ha
Jongwon
J
Kim
Sang Jun
SJ
Park
Kyong Soo
KS
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D005947:Glucose / Q000378:metabolism; D006085:Graft Survival; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012372:ROC Curve; D015203:Reproducibility of Results; D013552:Swine; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques; D014183:Transplantation, Heterologous
D015415:Biomarkers; D007328:Insulin; D000255:Adenosine Triphosphate; D013806:Theophylline; D005947:Glucose