[Effect of vinblastine nanoparticles on antiproliferation in human glioma cell lines BT325].
OBJECTIVE
To compare antiproliferation effects of vinblastine nanopraticles and vinblastine water solution in human glioma cell lines BT325.


METHODS
Vinblastine nanoparticles were prepared by emulsion polymerization process and using dextran as a stabilizing agent. It was characterized by means of morphology, size, drug entrapment efficiency and loading efficiency. Human glioma cell lines BT325 were treated with different concentrations of vinblastine nanoparticles and vinblastine water solution for 48 h, Antiproliferation effect was measured by MTT method. Morphological changes were observed by inverted microscope, transmission electron microscope and scanning electron microscope.


RESULTS
Mean diameter of VLB-PBCA-NP was about 74.4 nm, and drug entrapment efficiency and loading efficiency was 78.47% and 39.24%, respectively. Cell growth inhibition rate of vinblastine nanoparticles group and vinblastine water solution group in a concentration range (5-5 000 g x L(-1)) for 48 h was 41%, 49%, 73%, 83% and 28%, 33%, 54%, 60% respectively. Entrapment of VLB in NPS may distinctly degrade absorbency as compared to free drugs. Glioma cell BT325 which treated with VLB water solution were initial stage of apoptosis, and apoptosis body were forming. But VLB NPS-treated BT325 cells were intermediate or end stage, and missed structure integrality.


CONCLUSIONS
VLB-PBCA-NP and VLB water solution could inhibit the growth of human glioma cell lines BT325, and VLB nanoparticles have stronger inhibition effect compared with VLB water solution in the same dose. PBCA may be effective as promising carrier for the transport of vinblastine into the glioma cells.
Liu
Yu-Mei
YM
College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Ouyang
Wu-Qing
WQ
Zhang
Zi-Qiang
ZQ
Ma
Shu-Yan
SY
Yang
Bao-Ping
BP
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D014747:Vinblastine / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D014747:Vinblastine