title: M-PEIs nanogels: potential nonviral vector for systemic plasmid delivery to tumor cells.
abstract: Successfully systemic gene therapy has been hindered by vector-related limitations, including toxicity and inefficient gene delivery to tumor cells after intravenous administration. In this study, we evaluated the potential of spherical polyethylenimine nanogels (M-PEIs) as a novel vector for intravenous delivery of plasmids to tumor cells. M-PEIs provided a sustained release of plasmids up to 14 days and were also effective in protecting plasmids from enzymatic degradation in serum-conditioned media. M-PEIs showed no obvious cytotoxicity to mammalian cells in vitro as well as to liver, heart and kidney in mice after intravenous injection. Importantly, following intravenous administration of M-PEIs/plasmid complexes into human hepatocellular carcinoma xenograft-bearing mice, green fluorescence protein reporter gene expression was predominantly found in the tumor. This study indicates that M-PEIs may be a candidate for systemic delivery of plasmids into tumors.
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Dong
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: Y-B
authors.initials: YB
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Lu
authors.forename: D-M
authors.initials: DM
authors.lastname: Xu
authors.forename: B-H
authors.initials: BH
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: M
authors.initials: M
authors.lastname: Wu
authors.forename: S-D
authors.initials: SD
authors.lastname: Yao
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Guo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D011094:Polyethyleneimine