title: Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 1.
abstract: Seven in one blow: The efficient formation of mixed disulfides on the thiol-rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins.
authors.affiliation: Bioorganische Chemie, Gebäude NW1, Universität Bayreuth, 95440 Bayreuth, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Piontek
authors.forename: Petra
authors.initials: P
authors.lastname: Ring
authors.forename: Olaf
authors.initials: O
authors.lastname: Harjes
authors.forename: Christian
authors.initials: C
authors.lastname: Heinlein
authors.forename: Stefano
authors.initials: S
authors.lastname: Mezzato
authors.forename: Nelson
authors.initials: N
authors.lastname: Lombana
authors.forename: Claudia
authors.initials: C
authors.lastname: Pöhner
authors.forename: Markus
authors.initials: M
authors.lastname: Püttner
authors.forename: Daniel
authors.initials: D
authors.lastname: Varón Silva
authors.forename: Andreas
authors.initials: A
authors.lastname: Martin
authors.forename: Franz Xaver
authors.initials: FX
authors.lastname: Schmid
authors.forename: Carlo
authors.initials: C
authors.lastname: Unverzagt
mesh_terms: D004722:Endoribonucleases / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D006020:Glycopeptides / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D006023:Glycoproteins / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D008026:Ligation; D010084:Oxidation-Reduction; D015202:Protein Engineering; D017510:Protein Folding; D020673:Protein Renaturation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D004722:Endoribonucleases; C029493:ribonuclease C