title: Genome-based expression profiles as a single standardized microarray platform for the diagnosis of experimental interstitial cystitis: an array of 75 genes model.
abstract: OBJECTIVE
  To investigate the molecular signature underlying experimental interstitial cystitis (IC) using cDNA microarray.
  
  
  METHODS
  Microarray gene expression profiles are studied in bladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis.
  
  
  RESULTS
  Main findings are summarized as follows: firstly, a "75-gene" model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes.
  
  
  CONCLUSIONS
  Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC.
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and University of Chang Gung School of Medicine, Tao-Yuan, Taiwan. 3g7330@yahoo.com.tw
authors.forename: Ling-Hong
authors.initials: LH
authors.lastname: Tseng
authors.forename: Ilene
authors.initials: I
authors.lastname: Chen
authors.forename: Ming-Yang
authors.initials: MY
authors.lastname: Chen
authors.forename: Chyi-Long
authors.initials: CL
authors.lastname: Lee
authors.forename: Tsia-Shu
authors.initials: TS
authors.lastname: Lo
authors.forename: L Keith
authors.initials: LK
authors.lastname: Lloyd
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018856:Cystitis, Interstitial / Q000139:chemically induced / Q000175:diagnosis* / Q000235:genetics*; D036342:Ephrins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D020869:Gene Expression Profiling; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D052250:Nitric Oxide Synthase Type III / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010047:Ovalbumin; D051762:PAX2 Transcription Factor / Q000235:genetics; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism; D013373:Substance P; D059467:Transcriptome; D015854:Up-Regulation; D060449:Wnt Signaling Pathway / Q000235:genetics
chemical_list: D036342:Ephrins; D008070:Lipopolysaccharides; D051762:PAX2 Transcription Factor; C497319:Pax2 protein, mouse; D039961:Semaphorins; D013373:Substance P; D010047:Ovalbumin; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse