title: Directed evolution of enzymes: Library screening strategies.
abstract: Directed evolution has become the preferred engineering approach to generate tailor-made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures.
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: Leemhuis
authors.forename: Ronan M
authors.initials: RM
authors.lastname: Kelly
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen