Sensitivity to UV radiation in early life stages of the Mediterranean sea urchin Sphaerechinus granularis (Lamarck).
The sea urchin Sphaerechinus granularis was used to investigate the impact of relevant levels of UV-B radiation on the early life stages of a common Mediterranean free spawning benthic species. Sperm, eggs and embryos were exposed to a range of UV radiation doses. The resulting endpoints were evaluated in terms of fertilisation success, development and survival rates. Above a weighted UV radiation dose of 0.0029 kJ m(-2), fertilisation capability of irradiated sperm decreased rapidly. The exposure of the eggs to 0.0175 kJ m(-2) and more led to delayed and inhibited development with ensuing embryonic morphological abnormalities. One-day old larvae remained strongly sensitive to UV radiation as shown by the 50% decrease of the larval survival rate for a dose of 0.025 kJ m(-2) UVR. The elevated sensitivity of embryos to experimental UVR went along with a lack of significant amount of sunscreen compounds (e.g., mycosporine-like amino acids) in the eggs. The present results demonstrated that gamete viability and embryonic development may be significantly impaired by solar UV radiation in S. granularis, compromising in this way the reproduction of the species. Unless adaptive behavioural reproductive strategies exist, the influence of ambient UV radiation appears as a selective force for population dynamics of broadcast spawners in the shallow benthic Mediterranean environment.
Nahon
Sarah
S
UPMC Univ Paris 06, UMR 7621, LOBB, Observatoire Océanologique, F-66651, Banyuls/mer, France.
Castro Porras
Viviana A
VA
Pruski
Audrey M
AM
Charles
François
F
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004625:Embryo, Nonmammalian / Q000528:radiation effects; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D008297:Male; D008522:Mediterranean Sea; D010063:Ovum / Q000254:growth & development / Q000528:radiation effects; D012617:Sea Urchins / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects*; D013094:Spermatozoa / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000596:Amino Acids