title: Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice.
abstract: Nectin-1 is a Ca2+-independent Ig-like cell-cell adhesion molecule and an alphaherpesvirus receptor that binds to virion glycoprotein D by the first Ig-like domain. We have investigated the antiviral potentials of soluble forms of porcine nectin-1 to PRV infection by generating transgenic mice expressing different types of fusion protein. Previously, we reported that mice transgenic for a chimera that carried the entire ectodomain of porcine nectin-1 fused to the Fc portion of porcine IgG1 were more resistant than those transgenic for a chimera that carried the first Ig-like domain fused to the Fc portion. Recently, we generated transgenic mice expressing a fusion protein made of the first Ig-like domain fused to the Fc portion of human IgG1, and reported that they showed a microphthalmia. Here, two transgenic mouse lines expressing the fusion protein were challenged with PRV for comparing their resistances with those of transgenic mice expressing different types of fusion protein. Surprisingly, both transgenic mouse lines showed a high resistance to the viral infection, especially via the i.n. route. Significant resistance of the embryonic fibroblasts was also observed. Altogether, these findings indicated that the fusion protein consisting of the first Ig-like domain fused to the human Fc portion provided a marked resistance against PRV infection to the transgenic mice.
authors.affiliation: Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Tomioka
authors.forename: Masami
authors.initials: M
authors.lastname: Morimatsu
authors.forename: Keiko
authors.initials: K
authors.lastname: Amagai
authors.forename: Minako
authors.initials: M
authors.lastname: Kuramochi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kouda
authors.forename: Toshio
authors.initials: T
authors.lastname: Wada
authors.forename: Noritaka
authors.initials: N
authors.lastname: Kuboki
authors.forename: Etsuro
authors.initials: E
authors.lastname: Ono
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000276:immunology / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D000075983:Nectins; D017434:Protein Structure, Tertiary; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D034421:Sus scrofa
chemical_list: D015815:Cell Adhesion Molecules; D007141:Immunoglobulin Fc Fragments; C000619010:NECTIN1 protein, human; C000619015:Nectin1 protein, mouse; D000075983:Nectins; D011993:Recombinant Fusion Proteins