title: X-linked distal hereditary motor neuropathy maps to the DSMAX locus on chromosome Xq13.1-q21.
abstract: OBJECTIVE
  To clinically characterize and map the gene locus in a three-generation family with an X-linked adult-onset distal hereditary motor neuropathy.
  
  
  METHODS
  Microsatellite markers spanning the juvenile distal spinal muscular atrophy (DSMAX) locus were genotyped and analyzed using genetic linkage analysis. The promoter, untranslated and coding region of the gap junction beta1 (GJB1) gene was sequenced. Nine positional candidate genes were screened for disease mutations using high-resolution melt (HRM) analysis.
  
  
  RESULTS
  The family showed significant linkage to markers on chromosome Xq13.1-q21. Haplotype construction revealed a disease-associated haplotype between the markers DXS991 and DX5990. Sequence analysis excluded pathogenic changes in the coding and promoter regions of the GJB1 gene. Additional fine mapping in the family refined the DSMAX locus to a 1.44-cM interval between DXS8046 and DXS8114. HRM analysis did not identify disease-associated mutations in the coding region of nine candidate genes.
  
  
  CONCLUSIONS
  We have identified a family with adult-onset distal hereditary motor neuropathy that refines the locus reported for juvenile distal spinal muscular atrophy (DSMAX) on chromosome Xq13.1-q21. Exclusion of mutations in the coding and regulatory region of the GJB1 gene eliminated the CMTX1 locus as a cause of disease in this family. Nine positional candidate genes in the refined interval underwent mutation analysis and were eliminated as the pathogenic cause of DSMAX in this family. The syndrome in this family may be allelic to the juvenile distal spinal muscular atrophy first reported at this locus.
authors.affiliation: Northcott Neuroscience Laboratory, ANZAC Research Institute, Concord, New South Wales, Australia. marinak@anzac.edu.au
authors.forename: M
authors.initials: M
authors.lastname: Kennerson
authors.forename: G
authors.initials: G
authors.lastname: Nicholson
authors.forename: B
authors.initials: B
authors.lastname: Kowalski
authors.forename: K
authors.initials: K
authors.lastname: Krajewski
authors.forename: D
authors.initials: D
authors.lastname: El-Khechen
authors.forename: S
authors.initials: S
authors.lastname: Feely
authors.forename: S
authors.initials: S
authors.lastname: Chu
authors.forename: M
authors.initials: M
authors.lastname: Shy
authors.forename: J
authors.initials: J
authors.lastname: Garbern
mesh_terms: D000293:Adolescent; D000328:Adult; D002874:Chromosome Mapping; D041321:Chromosomes, Human, X; D017630:Connexins / Q000235:genetics; D050172:Genes, X-Linked; D008040:Genetic Linkage; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D018895:Microsatellite Repeats; D008875:Middle Aged; D009134:Muscular Atrophy, Spinal / Q000235:genetics*; D017421:Sequence Analysis; D000097002:Gap Junction beta-1 Protein
chemical_list: D017630:Connexins; D000097002:Gap Junction beta-1 Protein