Purification, crystallization and preliminary X-ray diffraction analysis of glutathionylated Trx1 C33S mutant from yeast.
Thioredoxins (Trxs) are a family of small redox-active proteins that are found in all living organisms. In Saccharomyces cerevisiae, two cytosolic Trxs (Trx1 and Trx2) and one mitochondrial Trx (Trx3) have previously been identified. In this work, cytosolic Trx1 containing a C33S mutant was overexpressed, purified, glutathionylated and crystallized using the hanging-drop vapour-diffusion method. A set of X-ray diffraction data was collected to 1.80 A resolution. The crystal belonged to space group P1, with unit-cell parameters a = 38.53, b = 38.81, c = 41.70 A, alpha = 72.91, beta = 87.51, gamma = 60.58 degrees.
Lou
Xiaochu
X
Institute of Protein Research, Tongji University, Shanghai, People's Republic of China.
Zhang
Yaru
Y
Bao
Rui
R
Zhou
Cong-Zhao
CZ
Chen
Yuxing
Y
D020134:Catalytic Domain; D003001:Cloning, Molecular; D003460:Crystallization; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D008956:Models, Chemical; D009154:Mutation; D054464:Peroxiredoxins; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013879:Thioredoxins / Q000737:chemistry*; D014961:X-Ray Diffraction
D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents; C103243:TRX1 protein, S cerevisiae; C516110:TRX2 protein, S cerevisiae; D013879:Thioredoxins; D054464:Peroxiredoxins; D005978:Glutathione