title: A miniaturized glucose biosensor for in vitro and in vivo studies.
abstract: A miniaturized wireless glucose biosensor has been developed to perform in vitro and in vivo studies. It consists of an external control subsystem and an implant sensing subsystem. The implant subsystem consists of a micro-processor, which coordinates circuitries of radio frequency, power regulator, command demodulator, glucose sensing trigger and signal read-out. Except for a set of sensing electrodes, the micro-processor, the circuitries and a receiving coil were hermetically sealed with polydimethylsiloxane. The electrode set is a substrate of silicon oxide coated with platinum, which includes a working electrode and a reference electrode. Glucose oxidase was immobilized on the surface of the working electrode. The implant subsystem bi-directionally communicates with the external subsystem via radio frequency technologies. The external subsystem wirelessly supplies electricity to power the implant, issues commands to the implant to perform tasks, receives the glucose responses detected by the electrode, and relays the response signals to a computer through a RS-232 connection. Studies of in vitro and in vivo were performed to evaluate the biosensor. The linear response of the biosensor is up to 15 mM of glucose in vitro. The results of in vivo study show significant glucose variations measured from the interstitial tissue fluid of a diabetes rat in fasting and non-fasting periods.
authors.affiliation: Biomedical Engineering Department, Chung-Yuan Christian University, Taiwan, R.O.C. g9275023@cycu.edu.tw
authors.forename: Yang-Li
authors.initials: YL
authors.lastname: Yang
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Huang
authors.forename: Ta-Feng
authors.initials: TF
authors.lastname: Tseng
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Lin
authors.forename: Shyh-Liang
authors.initials: SL
authors.lastname: Lou
mesh_terms: D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000175:diagnosis; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D004867:Equipment Design; D005949:Glucose Oxidase; D008904:Miniaturization; D051381:Rats; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D004129:Dimethylpolysiloxanes; D004800:Enzymes, Immobilized; C013830:baysilon; D005949:Glucose Oxidase