title: A serological and molecular investigation of American cutaneous leishmaniasis in dogs, three years after an outbreak in the Northwest of Paraná State, Brazil.
abstract: Classic and molecular (polymerase chain reaction--PCR) techniques were used to diagnose American cutaneous leishmaniasis in 149 dogs from an area in the northwest of Paraná State, Brazil, where an American cutaneous leishmaniasis outbreak occurred in 2002. The results were compared to a set of previously obtained results. Twenty-five dogs had positive indirect immunofluorescence (IIF) (titers > or = 40), including two animals with suggestive lesions. The percentage of dogs with positive IIF was similar to that found in a previous study. The cultures of the lesion, blood and bone marrow were negative for Leishmania. A direct search for the parasite in the lesions proved negative, although PCR tests were positive. The PCR did not detect the DNA of Leishmania (Viannia) in the blood, even for those that had positive PCR in a previous study. The follow up of the 27 dogs showed that the majority of them had maintained the same levels of antibodies that had been detected previously. There was a reduction in the number of dogs with lesions, probably due to the transmission control measures that were adopted after the outbreak.
authors.affiliation: Universidade Estadual de Maringá, Maringá, Brasil.
authors.forename: Gustavo Kiyoshi
authors.initials: GK
authors.lastname: Massunari
authors.forename: Evandra Maria
authors.initials: EM
authors.lastname: Voltarelli
authors.forename: Demilson Rodrigues dos
authors.initials: DR
authors.lastname: Santos
authors.forename: Ademar Rodrigues dos
authors.initials: AR
authors.lastname: Santos
authors.forename: Luiz Paschoal
authors.initials: LP
authors.lastname: Poiani
authors.forename: Otílio
authors.initials: O
authors.lastname: de Oliveira
authors.forename: Raul Jorge
authors.initials: RJ
authors.lastname: Violato
authors.forename: Ricardo
authors.initials: R
authors.lastname: Matsuo
authors.forename: Ueslei
authors.initials: U
authors.lastname: Teodoro
authors.forename: Maria Valdrinez Campana
authors.initials: MV
authors.lastname: Lonardoni
authors.forename: Thaís Gomes Verzignassi
authors.initials: TG
authors.lastname: Silveira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000235:genetics; D001853:Bone Marrow / Q000469:parasitology / Q000473:pathology; D001938:Brazil / Q000453:epidemiology; D003470:Culture Media; D016054:DNA, Protozoan / Q000097:blood / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary; D004197:Disease Reservoirs / Q000469:parasitology / Q000706:statistics & numerical data / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007892:Leishmania braziliensis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012424:Rural Population; D012883:Skin Ulcer / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D013997:Time Factors
chemical_list: D000913:Antibodies, Protozoan; D003470:Culture Media; D016054:DNA, Protozoan