Phosphotriesterase: a complementary tool for the selective detection of two organophosphate insecticides: chlorpyrifos and chlorfenvinfos.
This work shows the possibility of combining the high sensitivity of genetically-modified Drosophila melanogaster acetylcholinesterase (B394) with the ability of phosphotriesterase (PTE) to hydrolyse organophosphate compounds, in the aim of developing a biosensor selective to two insecticides of interest: chlorpyrifos and chlorfenvinfos. The studies clearly demonstrate that chlorfenvinfos is a substrate that acts as competitive inhibitor of PTE, therefore preventing the efficient hydrolysis of other pesticides, including chlorpyrifos. A bi-enzymatic sensor was designed by immobilizing both B394 and PTE in a polyvinylalcohol matrix. The sensor was shown to be able to discriminate between chlorpyrifos and chlorfenvinfos inhibitions.
Istamboulie
Georges
G
Université de Perpignan Via Domitia, IMAGES EA4218, Centre de Phytopharmacie, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France.
Fournier
Didier
D
Marty
Jean-Louis
JL
Noguer
Thierry
T
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002709:Chlorfenvinphos / Q000032:analysis*; D004390:Chlorpyrifos / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000032:analysis; D004331:Drosophila melanogaster / Q000201:enzymology; D005818:Genetic Engineering; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis; D044345:Phosphoric Triester Hydrolases / Q000378:metabolism*
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009943:Organophosphorus Compounds; D044345:Phosphoric Triester Hydrolases; D002709:Chlorfenvinphos; D004390:Chlorpyrifos