The evolution of Epstein-Barr virus inferred from the conservation and mutation of the virus glycoprotein gp350/220 gene.
To study variations of Epstein-Barr virus (EBV), we analyzed the gp350/220 gene for several cell lines and Japanese wild isolates using direct sequencing. The N-terminal region was highly conserved in all EBVs except for Jijoye/P3HR-1 and a few isolates. The variation of the region coincided with EBV types A and B (also referred to as types 1 and 2) and were, respectively, designated as the types a and b. The type A/a was detected in most Japanese cell lines and wild isolates, and was classified as China1 type with latent membrane protein (LMP) 1 gene. The type B/b was detected in only a few wild isolates with the Med and China2 types. The C-terminus had more diversity than the N-terminus and lacked the divergence between types A/a and B/b. The phylogenetic analyses of the gp350/220 and LMP1 genes may suggest a mode of EBV evolution into types A/a and B/b and then to LMP1 subtypes.
Kawaguchi
Asako
A
Divison of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, Yonago, Japan.
Kanai
Kyosuke
K
Satoh
Yukio
Y
Touge
Chizu
C
Nagata
Keiko
K
Sairenji
Takeshi
T
Inoue
Yoshitsugu
Y
D000595:Amino Acid Sequence; D016000:Cluster Analysis; D017124:Conserved Sequence; D004279:DNA, Viral / Q000235:genetics; D019143:Evolution, Molecular; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014763:Viral Matrix Proteins / Q000235:genetics*
D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins