title: High clusterin expression correlates with a poor outcome in stage II colorectal cancers.
abstract: The role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied in aggressive colon tumors; however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a prognostic marker. The study included 251 patients with stage II colorectal cancer. Tissue microarrays were constructed and immunohistochemistry done and correlated with clinical features and long term outcome. Dual immunofluorescence and confocal microscopy were used with terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling probes and clusterin antibody to assess the degree of co localization. Percentage epithelial cytoplasmic staining was higher in tumor compared with nonadjacent normal mucosa (P < 0.001). Within the stromal compartment, percentage cytoplamic staining and intensity was lower in tumor tissue compared with normal nonadjacent mucosa (P < or = 0.001). Survival was significantly associated with percentage epithelial cytoplasmic staining (P < 0.001), epithelial cytoplasmic staining intensity (P < 0.001), percentage stromal cytoplasmic staining (P = 0.002), and stromal cytoplasmic staining intensity (P < 0.001). Clusterin levels are associated with poor survival in stage II colorectal cancer.
authors.affiliation: Centre for Colorectal Disease, St Vincent's University Hospital, Elm Park, Dublin 4, Ireland.
authors.forename: David
authors.initials: D
authors.lastname: Kevans
authors.forename: Jane
authors.initials: J
authors.lastname: Foley
authors.forename: Martin
authors.initials: M
authors.lastname: Tenniswood
authors.forename: Kieran
authors.initials: K
authors.lastname: Sheahan
authors.forename: John
authors.initials: J
authors.lastname: Hyland
authors.forename: Diarmuid
authors.initials: D
authors.lastname: O'Donoghue
authors.forename: Hugh
authors.initials: H
authors.lastname: Mulcahy
authors.forename: Jacintha
authors.initials: J
authors.lastname: O'Sullivan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016009:Chi-Square Distribution; D051152:Clusterin / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric
chemical_list: D014408:Biomarkers, Tumor; D051152:Clusterin