title: Correspondence between neurological deficit, cerebral infarct size, and Rho-kinase activity in a rat cerebral thrombosis model.
abstract: Whether Rho-kinase activity is really associated with the pathogenesis of cerebral infarction remains unclear. To consider this question, we investigated correspondences between severity of neurological deficit, infarct size, amount of various marker proteins, and Rho-kinase activity in a rat cerebral infarction model. Sodium laurate was injected into the left internal carotid artery, inducing cerebral infarction in the ipsilateral hemisphere in rats. We prepared rats with various severities of neurological deficit (mild to severe) 3 days after injection of laurate, then measured infarct size and amount of various marker proteins, phosphorylation of substrates of Rho-kinase, myosin-binding subunit (MBS), myosin light chain (MLC), ezrin/radixin/moesin (ERM), and adducin using Western blot methods. First, infarct size increased corresponding to the severity of neurological deficit. Second, amounts of activating transcription factor 3, nestin, CD68, proliferating cell nuclear antigen, and heat shock protein 70 were increased, whereas neurofilament and myelin-associated glycoprotein were decreased corresponding to the severity of neurological deficit and infarct size. Finally, Rho-kinase activity (phospho-MBS/MBS, phospho-MLC/MLC, phospho-ERM/ERM, and phospho-adducin/adducin) was increased corresponding to the severity of neurological deficit and infarct size. Rho-kinase thus appears to play a crucial role in the pathogenesis of cerebral infarction.
authors.affiliation: Laboratory for Pharmacology, Asahi Kasei Pharma, 632-1 Mifuku, Izunokuni-shi, Shizuoka 410-2321, Japan. kawasaki.km@om.asahi-kasei.co.jp
authors.forename: Koh
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yano
authors.forename: Kunie
authors.initials: K
authors.lastname: Sasaki
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Tawara
authors.forename: Ichiro
authors.initials: I
authors.lastname: Ikegaki
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Satoh
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuriyama
authors.forename: Toshio
authors.initials: T
authors.lastname: Asano
authors.forename: Minoru
authors.initials: M
authors.lastname: Seto
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D020767:Intracranial Thrombosis / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D054460:rho-Associated Kinases