[Effect of ginsenoside Rh2 on transplanted-tumor and expression of JAM in mice].
OBJECTIVE
To discuss the anti-tumor activity of ginsenoside Rh2, we observed the expressions of the junction adhesion molecul (JAM) in transplanted-tumor in mice.


METHODS
The models of 40 transplanted-tumor mice that were established by subsequently injecting cancer ascite of mice (S180) with 0.2 mL per mouse into the preepipodite skin were divided into two groups. Experiment group was drenched with 2 mL ginsenoside Rh2 per mouse, equating to a dose 20 mg x kg(-1). Control group was drenched with 2 mL normal saline per mouse. The expression of JAM-1, JAM-2 in the lymphatics, blood vessels and tumours were observed by immunohistochemical staining.


RESULTS
The expression of JAM-1 on the cancer cells was significantly decreased in experiment group (IA 340.55) as compared with control group (IA 549.90, P<0.05). However, JAM-2 weakly expressed in both two groups. The density of blood vessels in which JAM-1, JAM-2 expressed showed 2.33 and 1.34 in control group, and 1.09 and 0.9 in experiment group respectively. Moreove, the density of lymph vessels were respectively 2.23 and 1.88 in control group compared with 0.99 and 0.79 in experiment group. The expression in blood vessels and lymph vessels in control group were significantly higher than those in experiment group, respectively (P<0.05).


CONCLUSIONS
Ginsenoside Rh2 can affect the tumor growth, further angiogenesis and lymphangiogenesis by down-regulating JAM expression in tumor.
Wang
Qiang
Q
Inner Mongolia Forestry General Hospital, Yakeshi 022150, China.
Wu
Mei-Qing
MQ
Zhao
Ling-Hui
LH
Yang
Hui-Ke
HK
Lv
Xiao-Hong
XH
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D007136:Immunoglobulins / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism; D062765:Junctional Adhesion Molecule B
D015815:Cell Adhesion Molecules; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; C498686:F11r protein, mouse; C498781:Jam2 protein, mouse; D062765:Junctional Adhesion Molecule B; C055305:ginsenoside Rh2