title: Staphylococcus aureus endoribonuclease III purification and properties.
abstract: Staphylococcus aureus ribonuclease III (Sa-RNase III) belongs to the enzyme family known to process double-stranded RNAs consisting of two turns of the RNA helix. Although the enzyme is thought to play a role in ribosomal RNA processing and gene regulation, the deletion of the rnc gene in S. aureus does not affect cell growth in rich medium. S. aureus RNase III acts in concert with regulatory RNAIII to repress the expression of several mRNAs encoding virulence factors. The action of the RNase is most likely to initiate the degradation of repressed mRNAs leading to an irreversible repression. In this chapter, we describe the overexpression and purification of recombinant RNase III from S. aureus, and we show that its biochemical properties are similar to the orthologous enzyme from Escherichia coli. Both enzymes similarly recognize and cleave different RNA substrates and RNA-mRNA duplexes.
authors.affiliation: Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, Strasbourg, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Chevalier
authors.forename: Eric
authors.initials: E
authors.lastname: Huntzinger
authors.forename: Pierre
authors.initials: P
authors.lastname: Fechter
authors.forename: Sandrine
authors.initials: S
authors.lastname: Boisset
authors.forename: François
authors.initials: F
authors.lastname: Vandenesch
authors.forename: Pascale
authors.initials: P
authors.lastname: Romby
authors.forename: Thomas
authors.initials: T
authors.lastname: Geissmann
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D043244:Ribonuclease III / Q000302:isolation & purification / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D013379:Substrate Specificity; D014774:Virulence
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D043244:Ribonuclease III