Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLCgamma and cooperate to modulate embryonic stem cell survival and proliferation.
Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.
Li
Ya-Li
YL
Institute of Molecular and Cellular Medicine, Kunming Medical College, China.
Wu
Guang-Zhi
GZ
Zeng
Li
L
Dawe
Gavin S
GS
Sun
Li
L
Loers
Gabriele
G
Tilling
Thomas
T
Cui
Shu-Sen
SS
Schachner
Melitta
M
Xiao
Zhi-Cheng
ZC
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D005562:Formazans / Q000378:metabolism; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006031:Glycosylation; D051379:Mice; D041681:NIH 3T3 Cells; D010740:Phospholipases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000378:metabolism; D014162:Transfection
D005562:Formazans; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; C079782:MTT formazan; D005647:Fucosyltransferases; D012799:Sialyltransferases; C029156:N-acetyllactosaminide alpha-2,3-sialyltransferase; D010740:Phospholipases; D001973:Bromodeoxyuridine