Fluorescent pteridine probes for nucleic acid analysis.
This chapter is focused on the fluorescent pteridine guanine analogs, 3MI and 6MI and on the pteridine adenine analog, 6MAP. A brief overview of commonly used methods to fluorescently label oligonucleotides reveals the role the pteridines play in the extensive variety of available probes. We describe the fluorescence characteristics of the pteridine probes as monomers and incorporated into DNA and review a variety of applications including changes in fluorescence intensity, anisotropies, time resolved studies, two photon excitation and single molecule detection.
Hawkins
Mary E
ME
National Institutes of Health, National Cancer Institute, Bethesda, MD 20892, USA.
D005456:Fluorescent Dyes / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis*; D011621:Pteridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011621:Pteridines