title: Adult progenitor cell transplantation influences contractile performance and calcium handling of recipient cardiomyocytes.
abstract: Adult progenitor cell transplantation has been proposed for the treatment of heart failure, but the mechanisms effecting functional improvements remain unknown. The aim of this study was to test the hypothesis that, in failing hearts treated with cell transplantation, the mechanical properties and excitation-contraction coupling of recipient cardiomyocytes are altered. Adult rats underwent coronary artery ligation, leading to myocardial infarction and chronic heart failure. After 3 wk, they received intramyocardial injections of either 10(7) green fluorescence protein (GFP)-positive bone marrow mononuclear cells or 5 x 10(6) GFP-positive skeletal myoblasts. Four weeks after injection, both cell types increased ejection fraction and reduced cardiomyocyte size. The contractility of isolated GFP-negative cardiomyocytes was monitored by sarcomere shortening assessment, Ca(2+) handling by indo-1 and fluo-4 fluorescence, and electrophysiology by patch-clamping techniques. Injection of either bone marrow cells or skeletal myoblasts normalized the impaired contractile performance and the prolonged time to peak of the Ca(2+) transient observed in failing cardiomyocytes. The smaller and slower L-type Ca(2+) current observed in heart failure normalized after skeletal myoblast, but not bone marrow cell, transplantation. Measurement of Ca(2+) sparks suggested a normalization of sarcoplasmic reticulum Ca(2+) leak after skeletal myoblast transplantation. The increased Ca(2+) wave frequency observed in failing myocytes was reduced by either bone marrow cells or skeletal myoblasts. In conclusion, the morphology, contractile performance, and excitation-contraction coupling of individual recipient cardiomyocytes are altered in failing hearts treated with adult progenitor cell transplantation.
authors.affiliation: Heart Science Centre, National Heart and Lung Institute, Imperial College London, Harefield Hospital, London, United Kingdom.
authors.forename: Joon
authors.initials: J
authors.lastname: Lee
authors.forename: Mark A
authors.initials: MA
authors.lastname: Stagg
authors.forename: Satsuki
authors.initials: S
authors.lastname: Fukushima
authors.forename: Gopal K R
authors.initials: GK
authors.lastname: Soppa
authors.forename: Urszula
authors.initials: U
authors.lastname: Siedlecka
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Youssef
authors.forename: Ken
authors.initials: K
authors.lastname: Suzuki
authors.forename: Magdi H
authors.initials: MH
authors.lastname: Yacoub
authors.forename: Cesare M N
authors.initials: CM
authors.lastname: Terracciano
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002118:Calcium / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology; D048429:Cell Size; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D004195:Disease Models, Animal; D022062:Electrophysiologic Techniques, Cardiac; D008297:Male; D032448:Myoblasts, Skeletal / Q000637:transplantation*; D009200:Myocardial Contraction / Q000502:physiology*; D009203:Myocardial Infarction / Q000628:therapy; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013318:Stroke Volume / Q000502:physiology
chemical_list: D002118:Calcium