title: [Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro].
abstract: OBJECTIVE
  To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.
  
  
  METHODS
  SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.
  
  
  RESULTS
  The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P < 0.05).
  
  
  CONCLUSIONS
  The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.
authors.affiliation: Department of Cell Biology, School of Basic Medical Science, The Second Hospital, Jilin University, Changchun, Jilin 130021, China. gxh_106@163.com
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Ye-ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Lian-wen
authors.initials: LW
authors.lastname: Zheng
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008297:Male; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects*; D019089:Stem Cell Factor / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D053605:Leukemia Inhibitory Factor; D019089:Stem Cell Factor; D016222:Fibroblast Growth Factor 2