Paclitaxel production using co-culture of Taxus suspension cells and paclitaxel-producing endophytic fungi in a co-bioreactor.
The co-culture of the suspension cells of Taxus chinensis var. mairei and its endophytic fungi, Fusarium mairei, in a 20-L co-bioreactor was successfully established for paclitaxel production. The co-bioreactor consists of two-unit tanks (10 L each) with a repairable separate membrane in the center, culturing Taxus suspension cells in one tank and growing fungi in another. By optimizing the co-culture conditions, there was a desirable yield of paclitaxel in Taxus cell cultures. The Taxus cell cultures by co-culture produced 25.63 mg/L of paclitaxel within 15 days; it was equivalent to a productivity of 1.71 mg/L per day and 38-fold higher than that by uncoupled culture (0.68 mg/L within 15 days). The optimum conditions for co-culture in the co-bioreactor were: B5 medium, inoculating fungi when Taxus cells had grown for 5 days in the co-bioreactor, hydrophilic separate membrane in the center of the co-bioreactor, and air flow rate of 1:0.85 v/v/m in fungus cultures.
Li
Yong-Cheng
YC
Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Tao
Wen-Yi
WY
Cheng
Long
L
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D017239:Paclitaxel / Q000096:biosynthesis*; D020946:Taxus / Q000378:metabolism* / Q000382:microbiology
D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D017239:Paclitaxel