title: Differential effects of ethanol in the nucleus accumbens shell of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar rats: a proteomics study.
abstract: The objective of this study was to determine the effects of ethanol injections on protein expression in the nucleus accumbens shell (ACB-sh) of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar (W) rats. Rats were injected for 5 consecutive days with either saline or 1 g/kg ethanol; 24 h after the last injection, rats were killed and brains obtained. Micro-punch samples of the ACB-sh were homogenized; extracted proteins were subjected to trypsin digestion and analyzed with a liquid chromatography-mass spectrometer procedure. Ethanol changed expression levels (1.15-fold or higher) of 128 proteins in NP rats, 22 proteins in P, and 28 proteins in W rats. Few of the changes observed with ethanol treatment for NP rats were observed for P and W rats. Many of the changes occurred in calcium-calmodulin signaling systems, G-protein signaling systems, synaptic structure and histones. Approximately half the changes observed in the ACB-sh of P rats were also observed for W rats. Overall, the results indicate a unique response to ethanol of the ACB-sh of NP rats compared to P and W rats; this unique response may reflect changes in neuronal function in the ACB-sh that could contribute to the low alcohol drinking behavior of the NP line.
authors.affiliation: Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, United States. wmcbride@iupui.edu
authors.forename: William J
authors.initials: WJ
authors.lastname: McBride
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Schultz
authors.forename: Mark W
authors.initials: MW
authors.lastname: Kimpel
authors.forename: Jeanette N
authors.initials: JN
authors.lastname: McClintick
authors.forename: Mu
authors.initials: M
authors.lastname: Wang
authors.forename: Jinsam
authors.initials: J
authors.lastname: You
authors.forename: Zachary A
authors.initials: ZA
authors.lastname: Rodd
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D009714:Nucleus Accumbens / Q000187:drug effects*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D000431:Ethanol