Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type I polyketide synthase gene.
Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746(T) revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746(T) was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.
Komaki
Hisayuki
H
Biomedicinal Information Research Center, Japan Biological Informatics Consortium, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan. hskomaki@nbrc.nite.go.jp
Izumikawa
Miho
M
Ueda
Jun-ya
JY
Nakashima
Takuji
T
Khan
Shams Tabrez
ST
Takagi
Motoki
M
Shin-ya
Kazuo
K
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010866:Natamycin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics* / Q000378:metabolism; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013057:Spectrum Analysis; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D000935:Antifungal Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D048630:Polyketide Synthases; D010866:Natamycin