title: [A simple method of separating outer hair cells in Guinea pig].
abstract: OBJECTIVE
  In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.
  
  
  METHODS
  The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.
  
  
  RESULTS
  A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.
  
  
  CONCLUSIONS
  If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
authors.affiliation: Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Xixun
authors.initials: X
authors.lastname: Kang
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
authors.forename: Wanrong
authors.initials: W
authors.lastname: Li
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zeng
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Chankkai
authors.initials: C
authors.lastname: Guo
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003051:Cochlea / Q000166:cytology*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology*