title: Intrahippocampal infusion of botulinum neurotoxin E (BoNT/E) reduces spontaneous recurrent seizures in a mouse model of mesial temporal lobe epilepsy.
abstract: Mesial temporal lobe epilepsy (MTLE) is one of the most common forms of human epilepsy, and it is often resistant to conventional antiepileptic drug (AED) therapy. Here we tested whether a single intrahippocampal administration of the synaptic blocker botulinum neurotoxin E (BoNT/E) is effective in reducing spontaneous recurrent seizures (SRS) in a mouse model of MTLE. Unilateral intrahippocampal injection of kainic acid (KA) in mice was used as a model of MTLE. Electroencephalography (EEG) recordings of SRS were performed during the chronic phase of epilepsy, before and after administration of either BoNT/E or vehicle. Frequency of SRS was significantly decreased for at least 5 days following BoNT/E, but not vehicle, infusion. Our findings demonstrate that BoNT/E can effectively reduce seizure incidence in a mouse model of MTLE.
authors.affiliation: Istituto di Neuroscienze, Consiglio Nazionale delle Ricerche, Pisa, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Antonucci
authors.forename: Yuri
authors.initials: Y
authors.lastname: Bozzi
authors.forename: Matteo
authors.initials: M
authors.lastname: Caleo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018726:Anti-Dyskinesia Agents / Q000627:therapeutic use*; D001905:Botulinum Toxins / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000379:methods; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D006624:Hippocampus / Q000187:drug effects*; D007608:Kainic Acid; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D018726:Anti-Dyskinesia Agents; D001905:Botulinum Toxins; D007608:Kainic Acid; C096326:botulinum toxin type E