title: Human polyomaviruses, WU and KI in HIV exposed children with acute lower respiratory tract infections in hospitals in South Africa.
abstract: BACKGROUND
  The importance of two recently identified polyomaviruses, WUV and KIV, as respiratory pathogens in populations with a high HIV prevalence needs to be defined, since human polyomaviruses can cause significant morbidity and mortality in patients with immunosuppression. Geographic distribution and disease association of WUV and KIV genotypes are not yet clearly defined.
  
  
  OBJECTIVE
  To investigate the prevalence of WUV and KIV in HIV-positive and HIV-negative patients with respiratory infections in hospitals in South Africa and determine their genotypes.
  
  
  METHODS
  Specimens from patients with acute respiratory infections from hospitals serving Pretoria were screened for WUV and KIV. Positive specimens were sequenced and subjected to phylogenetic analysis.
  
  
  RESULTS
  WUV was identified in (7%) and KIV in (1%) of mainly pediatric patients. Co-infections were common in WUV- and KIV-infected patients (71% and 66.6%, respectively); 57% of patients with WUV and 33% of patients with KIV were HIV-positive while the HIV prevalence in the respiratory virus patient group screened in this study was 33% WUV and KIV patients presented with moderate to severe lower respiratory tract disease. Four distinct and 2 unique WUV strains were identified clustering into 2 of 4 globally identified genotypes. KIV strains were identical to strains from Sweden.
  
  
  CONCLUSIONS
  WUV is frequently detected in HIV-infected patients with respiratory disease, but its role as respiratory pathogen remains uncertain.
authors.affiliation: Department of Medical Virology, University of Pretoria, South Africa. marietjie.venter@up.ac.za
authors.forename: Marietjie
authors.initials: M
authors.lastname: Venter
authors.forename: Adele
authors.initials: A
authors.lastname: Visser
authors.forename: Ria
authors.initials: R
authors.lastname: Lassauniere
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006761:Hospitals; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D013019:South Africa / Q000453:epidemiology; D055815:Young Adult
chemical_list: D004279:DNA, Viral