Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product.
The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variations accumulated in variant A and in reactivated variant B after transplantation. None of the variant-A viruses encoded the signal peptide found in the B variant. U83 gene sequencing suggested that the variant A and B groups were separate, and that the variant B viruses could be further divided into the HST-Z29 type and another type with a shorter signal peptide. In a eukaryotic expression system, the HST-Z29 type of U83 gene product was secreted into the medium, a frame-shifted HST-Z29 type was partially secreted, and the variant-A type and a first-methionine knockout of the HST-Z29 type were not secreted.
Sjahril
R
R
Department of Infectious Disease Control, Graduate School of Medicine G5, Osaka University, 2-2 Yamada-Oka Suita, Osaka 565-0871, Japan.
Isegawa
Yuji
Y
Tanaka
T
T
Nakano
K
K
Yoshikawa
T
T
Asano
Y
Y
Ohshima
A
A
Yamanishi
K
K
Sugimoto
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015023:Democratic Republic of the Congo; D005787:Gene Frequency; D014644:Genetic Variation; D005858:Germany; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D019349:Roseolovirus Infections / Q000378:metabolism* / Q000821:virology; D016415:Sequence Alignment; D017385:Sequence Homology; D014481:United States; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D018925:Chemokines; C120137:U83 protein, Human herpesvirus 6; D014764:Viral Proteins