title: A cooperative polymer-DNA microarray approach to biomaterial investigation.
abstract: In this study, polymer microarrays were used for the rapid identification of polymer substrates upon which a suspension cell line would both adhere and proliferate giving a detailed and rapid understanding of cell-biomaterial interactions. Analysis demonstrated that suspension K562 human erythroleukemic cells, which normally grow in suspension, adhered and proliferated on several different polymers. Phenotypic and transcriptomic analysis techniques allowed examination of the interaction between cells and polymers permitting the elucidation of putative links between phenotypic responses to cell-biomaterial interactions and global gene expression.
authors.affiliation: School of Chemistry, University of Edinburgh, EH9 3JJ, Edinburgh, UK.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pernagallo
authors.forename: Juan Jose
authors.initials: JJ
authors.lastname: Diaz-Mochon
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D000180:Acrylic Resins / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics; D049109:Cell Proliferation; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D020014:K562 Cells; D046228:Microarray Analysis / Q000379:methods*; D033401:Microscopy, Acoustic; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011108:Polymers / Q000378:metabolism*; D011140:Polyurethanes / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D011108:Polymers; D011140:Polyurethanes