title: Determination of D,L-serine in midbrain of Parkinson's disease mouse by capillary electrophoresis with in-column light-emitting diode induced fluorescence detection.
abstract: A capillary electrophoresis method with in-column light-emitting diode induced fluorescence detection is described for simultaneous determination of D,L-serine in the midbrain of a Parkinson's disease mouse. D,L-Serine was derivatized with fluorescein isothiocyanate, and chiral separation and determination of D,L-serine derivatives were performed on a laboratory-built capillary electrophoresis system with in-column light-emitting diode induced fluorescence detector using gamma-cyclodextrin as chiral selector. Using this method, the levels of D- and L-serine in the midbrains of Parkinson's disease mice were determined. When compared to controls, the levels of D- and L-serine showed significant differences. The result suggested that the biosynthesis and the transportation of endogenous D,L-serine may participate in Parkinson's disease pathogenesis.
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi, Normal University, Guilin, China.
authors.forename: Shuting
authors.initials: S
authors.lastname: Li
authors.forename: Qinglei
authors.initials: Q
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis* / Q000737:chemistry; D054023:Lasers, Semiconductor; D008297:Male; D008636:Mesencephalon / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010300:Parkinson Disease / Q000378:metabolism*; D012694:Serine / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D004573:Electrolytes; D012694:Serine; D016650:Fluorescein-5-isothiocyanate