title: Notch mediates Wnt and BMP signals in the early separation of smooth muscle progenitors and blood/endothelial common progenitors.
abstract: During embryonic development in amniotes, the extraembryonic mesoderm, where the earliest hematopoiesis and vasculogenesis take place, also generates smooth muscle cells (SMCs). It is not well understood how the differentiation of SMCs is linked to that of blood (BCs) and endothelial (ECs) cells. Here we show that, in the chick embryo, the SMC lineage is marked by the expression of a bHLH transcription factor, dHand. Notch activity in nascent ventral mesoderm cells promotes SMC progenitor formation and mediates the separation of SMC and BC/EC common progenitors marked by another bHLH factor, Scl. This is achieved by crosstalk with the BMP and Wnt pathways, which are involved in mesoderm ventralization and SMC lineage induction, respectively. Our findings reveal a novel role of the Notch pathway in early ventral mesoderm differentiation, and suggest a stepwise separation among its three main lineages, first between SMC progenitors and BC/EC common progenitors, and then between BCs and ECs.
authors.affiliation: RIKEN Center for Developmental Biology, Laboratory for Early Embryogenesis, Kobe, Hyogo 650-0047, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shin
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nagai
authors.forename: Guojun
authors.initials: G
authors.lastname: Sheng
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology* / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D002645:Chickens; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D011784:Quail; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D019485:Bone Morphogenetic Proteins; D051880:Receptors, Notch; D051153:Wnt Proteins