title: A single nucleotide polymorphism in the promoter of the LOXL1 gene and its relationship to pelvic organ prolapse and preterm premature rupture of membranes.
abstract: Pelvic organ prolapse and preterm premature rupture of membranes, the 2 conditions which have in common weakening of the tensile strength of tissues, are thought to be caused, in part, by abnormal extracellular matrix synthesis and/or catabolism. We identified a new single nucleotide polymorphism (NT_010194(LOXL1):g.45008784A>C) in the promoter of the LOXL1 gene, which is essential for elastin synthesis. Promoter studies showed that the minor "C'' allele had significantly greater activity than the major "A'' allele. Case-control studies examined the association of the alleles of this single nucleotide polymorphism with pelvic organ prolapse and preterm premature rupture of membranes. When comparing allele frequencies and genotypes in pelvic organ prolapse cases versus controls, no significant associations were found. A case-control study conducted in African American neonates also found no significant associations between the promoter alleles and preterm premature rupture of membranes. We conclude that a functional single nucleotide polymorphism exists in the promoter region of the LOXL1 gene. Association studies suggest that the promoter single nucleotide polymorphism does not contribute significantly to risk of pelvic organ prolapse or preterm premature rupture of membranes.
authors.affiliation: Department of Obstetrics and Gynecology, Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA.
authors.forename: Georgia
authors.initials: G
authors.lastname: Ferrell
authors.forename: Minyan
authors.initials: M
authors.lastname: Lu
authors.forename: Paul
authors.initials: P
authors.lastname: Stoddard
authors.forename: Mary D
authors.initials: MD
authors.lastname: Sammel
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Jerome F
authors.initials: JF
authors.lastname: Strauss
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Matthews
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D008875:Middle Aged; D017773:Pelvic Floor / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D047928:Premature Birth / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014596:Uterine Prolapse / Q000201:enzymology / Q000235:genetics*
chemical_list: D000594:Amino Acid Oxidoreductases; C445313:LOXL1 protein, human