Elevated transrenal DNA (cell-free urine DNA) in patients with urinary tract infection compared to healthy controls.
OBJECTIVE
To study whether levels of transrenal DNA (Tr-DNA) are influenced by the presence of urinary tract infections (UTI).


METHODS
Tr-DNA concentrations were measured in 124 donors and 55 patients with UTI. Two methods for DNA extraction were compared.


RESULTS
UTI patients showed higher concentrations than the donors (normal range: 0-147 GE/mL). QIAamp MiniElute Virus Spin Kit was more efficient than QIAamp DNA Blood Mini Kit.


CONCLUSIONS
Increased concentrations of Tr-DNA urine DNA are shown in presence of UTI.
García Moreira
Vanessa
V
Servicio de Bioquímica Clínica, Hospital Universitario Central de Asturias, Spain.
Prieto García
Belén
B
de la Cera Martínez
Tamara
T
Alvarez Menéndez
Francisco V
FV
D000328:Adult; D000368:Aged; D004247:DNA / Q000652:urine*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000652:urine*; D055815:Young Adult
D004247:DNA