A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.
A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature.
González-Vera
Juan A
JA
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Luković
Elvedin
E
Imperiali
Barbara
B
D064127:Aurora Kinases; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D005456:Fluorescent Dyes; D019151:Peptide Library; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005456:Fluorescent Dyes; D019151:Peptide Library; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases