[Study on HPLC fingerprint of Prunella vulgaris].
OBJECTIVE
To establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.


METHODS
A sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.


RESULTS
The similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.


CONCLUSIONS
This method is available for quality evaluation and control the quality of P. vulgaris.
Sun
Wei-Guang
WG
Guangzhou Xingqun Pharmaceutical Ltd. Co., Guangzhou 510288, China.
Ke
Xue-Hong
XH
Li
Yuan
Y
Hua
Ru-Feng
RF
Chen
Jin-Fu
JF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D031347:Prunella / Q000737:chemistry*
D004365:Drugs, Chinese Herbal