title: [Studies on constituents of rootsanel leaves from Desmodium blandum and their cytotoxic activity against growth of several tumor cells].
abstract: OBJECTIVE
  To investigate the chemical constituents of Desmodium blandum and their cytotoxic activity against the growth of several tumor cells.
  
  
  METHODS
  Various chromatographic techniques including silica gel, Sephadex LH-20 column chromatography were employed for the isolation and purification of the constituents. The structures of compounds were elucidated by spectral analyses (IR, UV, NMR, MS). Their cytotoxic activity was then studied.
  
  
  RESULTS
  Eight compounds were isolated from the stems of D. blandum and identified as N, N-dimethyltryptamine (1), 5-methoxy-N, N-dimethyltryptamine (2), citrusinol (3), yukovanol (4), (Z)-1-(4-hydroxy-2, 3-dimethoxyphenyl)-3-(4-hydroxyphenyl) propene (5), (Z)-1-(3-hydroxy-2, 4-dimethoxy-phenyl)-3-(4-hydroxy-3-methoxy-phenyl) propene (6), methylprotocatechuate (7), katuranin (8).
  
  
  CONCLUSIONS
  Among these compounds, compound 6 was isolated from D. blandum for the first time. In the MTT test, compounds 2 and 6 exhibit cytotoxic activities against the KB cell, and compounds 3 and 6 exhibit the same activities against the HepG2 cell.
authors.affiliation: State Key Laboratory of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, China. ganning@nbu.edu.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: He
mesh_terms: D000475:Alkenes / Q000737:chemistry / Q000494:pharmacology; D001555:Benzene Derivatives / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity*; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D004130:N,N-Dimethyltryptamine / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared
chemical_list: C540483:(Z)-1-(3-hydroxy-2,4-dimethoxy-phenyl)-3-(4-hydroxy-3-methoxy-phenyl)propene; D000475:Alkenes; D001555:Benzene Derivatives; D004365:Drugs, Chinese Herbal; D004130:N,N-Dimethyltryptamine