title: Molecular diversity of Mycobacterium tuberculosis strains in a slum area of Rio de Janeiro, Brazil.
abstract: This retrospective molecular study involving restriction fragment length polymorphism, using insertion sequence 6110 as a marker, was conducted in order to provide an initial insight into the genetic diversity of Mycobacterium tuberculosis strains isolated in the slums of the Complexo de Manguinhos, located in the city of Rio de Janeiro, Brazil. Of the 67 strains evaluated, 23 (34.3%) were found to belong to clusters (total clusters, 10). Household and social chains of transmission were associated with clustering, in 20% and 60%, respectively. Living in the Conjunto Habitacional Programado 2 slum was associated with clustering. Although not significant, it is relevant that 26% of the clustered strains presented primary resistance. These findings, although possibly underestimating the prevalence due to the failure to analyze all strains, could help improve the local tuberculosis control program.
authors.affiliation: Centro de Pesquisa Leônidas & Maria Deane, Fundação Instituto Oswaldo Cruz, Manaus, AM, Brazil.
authors.forename: Joycenea Matsuda
authors.initials: JM
authors.lastname: Mendes
authors.forename: Silvia Maria Almeida
authors.initials: SM
authors.lastname: Machado
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Lourenço
authors.forename: Rosa Maria Carvalho
authors.initials: RM
authors.lastname: Ferreira
authors.forename: Leila de Souza
authors.initials: Lde S
authors.lastname: Fonseca
authors.forename: Maria Helena Feres
authors.initials: MH
authors.lastname: Saad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D016000:Cluster Analysis; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D011204:Poverty Areas; D012189:Retrospective Studies; D014376:Tuberculosis / Q000235:genetics* / Q000382:microbiology / Q000635:transmission; D055815:Young Adult
chemical_list: D005819:Genetic Markers