title: Genetic analysis and fine mapping of two genes for grain shape and weight in rice.
abstract: To identify genetic loci controlling grain weight, an elite indica rice variety, Baodali, with large grains was identified and used in this study. Its derived F(2), F(3) and BC(2)F(2) with another japonica rice variety Zhonghua 11 were used as mapping populations. Linkage analyses demonstrated that two genes controlling grain weight, designated as GW3 and GW6, were mapped to chromosome 3 and chromosome 6, respectively. Fine mapping delimited GW3 to a 122 kb physical distance between two sequence tagged site markers (WGW16 and WGW19) containing 16 open reading frames annotated by The Institute for Genomic Research (http://www.tigr.org). GW6 was further mapped between two simple sequence repeat markers (RM7179 and RM3187). These results are useful for both marker assisted selection of grain weight, and for further cloning of GW genes, which will contribute to the dissection of the molecular mechanism underlying grain weight in rice.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.forename: Lilian
authors.initials: L
authors.lastname: Ma
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Liwen
authors.initials: L
authors.lastname: Wu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Gao
authors.forename: Guangheng
authors.initials: G
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D001483:Base Sequence; D018533:Biomass; D020090:Chromosome Segregation; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D017343:Genes, Plant; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics; D012275:Oryza / Q000033:anatomy & histology* / Q000235:genetics*; D010641:Phenotype; D020161:Physical Chromosome Mapping; D040641:Quantitative Trait Loci / Q000235:genetics; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000033:anatomy & histology* / Q000235:genetics*