title: Inhibitory effects of an aqueous extract of Cornus kousa Burg. leaves on TNF-alpha-induced chemokine expression and monocyte adhesion to human colonic epithelial cells.
abstract: An aqueous extract of Cornus kousa Burg. leaves (ACK) that contained high amount of polyphenols showed significant antioxidant activity against diphenylpicrylhydrazyl (DPPH) radicals and TNF-alpha-generated reactive oxygen species. ACK at concentrations of 10 and 50 microg/mL significantly inhibited TNF-alpha-induced adhesion of U937 pre-monocytic cells to HT-29 colon epithelial cells in a concentration-dependent manner. The reduced adhesion by ACK correlated with the suppressed expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8, the major inflammatory bowel disease (IBD)-associated chemokines. Moreover, ACK significantly suppressed TNF-alpha-induced translocation of redox-sensitive nuclear factor (NF)-kappaB as well as degradation of cytosolic I-kappaBalpha. The effective concentrations of ACK were much lower than that of 5-aminosalicylic acid (3.06 mg/mL), which is an active metabolite of sulfasalazine, a well-known drug used in the treatment of IBD. The results indicate that ACK may provide a potential benefit for the prevention and treatment of inflammatory diseases such as IBD.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Korea.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Babu
authors.forename: Dinesh
authors.initials: D
authors.lastname: Thapa
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Ah Ra
authors.initials: AR
authors.lastname: Kim
authors.forename: Young Heui
authors.initials: YH
authors.lastname: Kim
authors.forename: Hong Chul
authors.initials: HC
authors.lastname: Yang
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D018932:Chemokine CCL2 / Q000378:metabolism; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D029768:Cornus / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D005419:Flavonoids / Q000032:analysis; D019073:HT29 Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D019804:Mesalamine / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves; D059808:Polyphenols; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D020298:U937 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D018925:Chemokines; D005419:Flavonoids; D020672:I-kappa B Proteins; D016209:Interleukin-8; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D019804:Mesalamine