Usefulness of haptoglobin and serum amyloid A proteins as biomarkers for atherothrombotic ischemic stroke diagnosis confirmation.
OBJECTIVE
To identify protein biomarkers in order to classify ischemic stroke subtypes using proteomic analysis and immunoenzymatic tools for clinical validation.


RESULTS
We performed a proteomic analysis in serum samples of 24 patients with ischemic stroke (12 atherothrombotic patients and 12 cardioembolic patients). In this study, based on two-dimensional electrophoresis and mass spectrometry we found four spots whose expression intensity was at least four times stronger in atherothrombotic patients than in cardioembolic patients. These spots were identified as haptoglobin related protein, serum amyloid A (two spots) and haptoglobin alpha chain. We validated the possible value of haptoglobin and serum amyloid A in a larger series of patients (n=262) with ischemic stroke using ELISA techniques. Haptoglobin levels >1040microg/mL identified atherothrombotic patients with 95% sensitivity and 88% specificity whereas serum amyloid A levels >160microg/mL identified atherothrombotic patients with 91% sensitivity and 83% specificity.


CONCLUSIONS
Haptoglobin and serum amyloid A are useful biomarkers for atherothrombotic ischemic stroke diagnosis confirmation.
Brea
David
D
Department of Neurology, Hospital Clínico Universitario, University of Santiago de Compostela, Spain.
Sobrino
Tomás
T
Blanco
Miguel
M
Fraga
Máximo
M
Agulla
Jesús
J
Rodríguez-Yáñez
Manuel
M
Rodríguez-González
Raquel
R
Pérez de la Ossa
Natalia
N
Leira
Rogelio
R
Forteza
Jerónimo
J
Dávalos
Antonio
A
Castillo
José
J
D000368:Aged; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006242:Haptoglobins / Q000096:biosynthesis*; D006801:Humans; D007511:Ischemia / Q000097:blood / Q000175:diagnosis; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D000685:Serum Amyloid A Protein / Q000096:biosynthesis*; D020521:Stroke / Q000097:blood* / Q000175:diagnosis; D013927:Thrombosis / Q000097:blood / Q000175:diagnosis
D015415:Biomarkers; D006242:Haptoglobins; D000685:Serum Amyloid A Protein