Determination of D,L-serine in midbrain of Parkinson's disease mouse by capillary electrophoresis with in-column light-emitting diode induced fluorescence detection.
A capillary electrophoresis method with in-column light-emitting diode induced fluorescence detection is described for simultaneous determination of D,L-serine in the midbrain of a Parkinson's disease mouse. D,L-Serine was derivatized with fluorescein isothiocyanate, and chiral separation and determination of D,L-serine derivatives were performed on a laboratory-built capillary electrophoresis system with in-column light-emitting diode induced fluorescence detector using gamma-cyclodextrin as chiral selector. Using this method, the levels of D- and L-serine in the midbrains of Parkinson's disease mice were determined. When compared to controls, the levels of D- and L-serine showed significant differences. The result suggested that the biosynthesis and the transportation of endogenous D,L-serine may participate in Parkinson's disease pathogenesis.
Li
Shuting
S
College of Chemistry and Chemical Engineering, Guangxi, Normal University, Guilin, China.
Yu
Qinglei
Q
Lu
Xin
X
Zhao
Shulin
S
D000818:Animals; D001923:Brain Chemistry; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis* / Q000737:chemistry; D054023:Lasers, Semiconductor; D008297:Male; D008636:Mesencephalon / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010300:Parkinson Disease / Q000378:metabolism*; D012694:Serine / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
D004573:Electrolytes; D012694:Serine; D016650:Fluorescein-5-isothiocyanate