title: Piecemeal microautophagy of the nucleus: genetic and morphological traits.
abstract: Nucleus-vacuole (NV) junctions are formed in Saccharomyces cerevisiae through interactions between Vac8 in the vacuole membrane and Nvj1 in the perinuclear ER. Upon starvation, vesicles containing part of the nucleus emanate from these contact sites and finally pinch off into invaginations of the vacuole. Due to its morphological similarity to microautophagy this process had been termed "piecemeal microautophagy of the nucleus" (PMN). We recently discovered that a number of ATG genes required for macroautophagy and micropexophagy are also required for PMN and accordingly named it micronucleophagy. Therefore, PMN represents a novel model system to investigate the functions of the highly conserved but poorly understood core autophagic apparatus. We here extend the morphological analysis of PMN using immunogold and freeze fracture electron microscopy.
authors.forename: Roswitha
authors.initials: R
authors.lastname: Krick
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Mühe
authors.forename: Tanja
authors.initials: T
authors.lastname: Prick
authors.forename: Monika
authors.initials: M
authors.lastname: Bredschneider
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bremer
authors.forename: Dirk
authors.initials: D
authors.lastname: Wenzel
authors.forename: Eeva-Liisa
authors.initials: EL
authors.lastname: Eskelinen
authors.forename: Michael
authors.initials: M
authors.lastname: Thumm
mesh_terms: D001343:Autophagy; D002467:Cell Nucleus / Q000235:genetics* / Q000648:ultrastructure; D005614:Freeze Fracturing; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure