Cereblon is expressed in the retina and binds to voltage-gated chloride channels.
The function of the central nervous system depends on the correct regulation of ion channels by interacting proteins. Here, we identified cereblon as a new interactor of the voltage-gated chloride channel ClC-2. A distal region of the ClC-2 C-terminus interacts with the Lon domain of cereblon. Cereblon is expressed in several brain regions including the retina. There, we detected cereblon in nuclear and synaptic layers and localized the protein in the same subcellular region of bipolar cell bodies previously reported to express ClC-2. Our data suggest that cereblon might be associated with voltage-gated chloride channels in the central nervous system.
Hohberger
Bettina
B
Institut für Biochemie (Emil-Fischer-Zentrum), Friedrich-Alexander-Universität Erlangen-Nürnberg, Fahrstrasse 17, 91054 Erlangen, Germany.
Enz
Ralf
R
D049069:ATP-Dependent Proteases; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005982:Glutathione Transferase / Q000378:metabolism; D007150:Immunohistochemistry; D015640:Ion Channel Gating / Q000235:genetics / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D043743:Ubiquitin-Protein Ligase Complexes
C503910:CRBN protein, rat; D002352:Carrier Proteins; D018118:Chloride Channels; D002712:Chlorides; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D005982:Glutathione Transferase; D049069:ATP-Dependent Proteases