title: Determination of zinc using carbonic anhydrase-based fluorescence biosensors.
abstract: This chapter summarizes the use of carbonic anhydrase (CA)-based fluorescent indicators to determine free zinc in solution, in cells, and in subcellular organelles. Expression (both in situ and in vitro) and preparation of CA-based indicators are described, together with techniques of their use, and procedures to minimize contamination. Recipes for zinc buffers are supplied.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bozym
authors.forename: Tamiika K
authors.initials: TK
authors.lastname: Hurst
authors.forename: Nissa
authors.initials: N
authors.lastname: Westerberg
authors.forename: Andrea
authors.initials: A
authors.lastname: Stoddard
authors.forename: Carol A
authors.initials: CA
authors.lastname: Fierke
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Frederickson
authors.forename: Richard B
authors.initials: RB
authors.lastname: Thompson
mesh_terms: D015374:Biosensing Techniques; D002256:Carbonic Anhydrases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D015032:Zinc / Q000032:analysis*
chemical_list: D002256:Carbonic Anhydrases; D015032:Zinc