title: [Different cytotoxicity of NK cells against high and low expression of ATP-binding cassette transporter (ABCG(2)) of human multi-drug resistant nasopharyngeal carcinoma cells].
abstract: OBJECTIVE
  To investigate the different cytotoxicity sensitivity of high and low expression of ATP-binding cassette transporter (ABCG(2)) of human multi-drug resistant nasopharyngeal carcinoma CNE2/DDP cell line (ABCG(2)(High) cell and ABCG(2)(Low cell)) to NK cells.
  
  
  METHODS
  ABCG(2)(High)CNE2/DDP cells, ABCG(2)(Low) CNE2/DDP cells and NK cells were isolated by magnetic activated cell sorting (MACS). The cytotoxic effects of NK cells against K562, ABCG(2)(High) and ABCG(2)(Low)CNE2/DDP cells were detected with LDH releasing assay. The apoptosis rate and expression of NKG2D-ligands on target cells were evaluated by FCM.
  
  
  RESULTS
  The expression of ABCG(2) in ABCG(2)(High) CNE2/DDP and ABCG(2)(Low) CNE2/DDP cells was (91.40+/-2.32)% and (1.70+/-0.24)%, respectively. More than 90% of the isolated NK cells were CD3(-)CD16(+)CD56(+) cells. As for the cytotoxicity of NK cells against ABCG(2)(Low), ABCG(2)(High)CNE2/DDP and K562 cells at the E vs T ratio of 10 vs 1 and 20 vs 1, ABCG(2)(High) CNE2/DDP cells possessed highest cytotoxic sensitivity. The apoptosis rate of ABCG(2)(High) CNE2/DDP cells and ABCG(2)(Low)CNE2/DDP cells was (12.90+/-1.51)% and (6.13+/-0.85)%, respectively. The expression of five kinds of NKG2D ligand of ABCG(2);(High)CNE2/DDP cells is higher than that of ABCG(2)(Low)CNE2/DDP cells.
  
  
  CONCLUSIONS
  ABCG(2)(High) CNE2/DDP cells are more sensitive to NK cells than ABCG(2)(Low) CNE2/DDP cells in cytotoxicity because the expression of NKG2D ligands of ABCG(2)(High)CNE2/DDP cells is higher than that of ABCG(2)(Low)CNE2/DDP cells, which results in the cytotoxic sensitivity of increased tumor cells to NK cells.
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou, China. hyx6610@163.com
authors.forename: Yu-xian
authors.initials: YX
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan-ju
authors.initials: YJ
authors.lastname: Li
authors.forename: Chao-yang
authors.initials: CY
authors.lastname: Song
authors.forename: Kun-yuan
authors.initials: KY
authors.lastname: Guo
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D019008:Drug Resistance, Neoplasm; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000502:physiology*; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D009363:Neoplasm Proteins