Immunoelectron microscopic analysis of the distribution of tyrosine kinase receptor B in olfactory axons.
To determine the morphological basis for the neurotrophic effects of brain-derived neurotrophic factor (BDNF) in the primary olfactory pathway (POP), tyrosine kinase receptor B (TrkB), a membrane-bound receptor for BDNF, was identified and localized in axons of olfactory receptor cells (ORC) of neonatal rat olfactory mucosa using immuno-histochemical and -cytochemical techniques. Initially, the immunospecificity of an anti-TrkB antibody that had been used as a specific antibody for full-length TrkB was confirmed in the olfactory mucosa. Then, a combination of a reduced osmium-LR-White and post-embedding immunogold technique was applied to ORC axons in the lamina propria just beneath the olfactory epithelium. Immunogold particles, which indicate TrkB immunoreactivity, were noted either in close association with the plasma membranes of ORC axons, and designated plasma-lemmal (PL), or within their cytoplasm, and designated cytoplasmic (CP). Most PL particles were seen in the CP portion of the axonal plasma membranes, suggesting that the anti-TrkB antibody binds to the membrane-inserted TrkB that acts as a functional receptor. Some CP particles were on vesicular structures. Quantitative analysis demonstrated that the ratio of CP to PL particles was 7:3, and this ratio was constant between animals examined (n = 5). Because membrane proteins are wrapped in vesicles and transported within the axonal cytoplasm and inserted into the plasma membrane to function there, the present study suggests that TrkB is transported within the cytoplasm of ORC axons and is positioned as a functional receptor for BDNF in their membranes.
Hasegawa
Rumi
R
Laboratory of Anatomy, Faculty and Graduate School of Health Sciences, Kyorin University, Tokyo, Japan.
Takami
Shigeru
S
Nishiyama
Fumiaki
F
D000818:Animals; D000918:Antibody Specificity; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D008297:Male; D016253:Microscopy, Immunoelectron / Q000379:methods; D009831:Olfactory Mucosa / Q000378:metabolism / Q000648:ultrastructure; D009832:Olfactory Nerve / Q000378:metabolism* / Q000648:ultrastructure*; D018034:Olfactory Receptor Neurons / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D020813:Receptor, trkB