title: [Interaction of fluorine, calcium and iodine on weight of rats].
abstract: OBJECTIVE
  To study the interaction of fluorine (F), calcium (Ca) and iodine (I) on body weight of rats.
  
  
  METHODS
  One-month-old Wistar rats were randomly divided into 8 groups: moderate concentrations of F, Ca, I (group 1); moderate concentrations of F, I and high concentration of Ca (group 2); moderate concentrations of Ca, I and high concentration of F (group 3); moderate concentration of I and high concentrations of F, Ca (group 4); moderate concentrations of F, Ca and low concentration of I (group 5); moderate concentration of F,high concentration of Ca and low concentration of I (group 6); moderate concentration of Ca, high concentration of F and low concentration of I (group 7); high concentrations of F, Ca and low concentration of I (group 8) based on 2 x 2 x 2 factorial design. The moderate concentration of F was 90 microg/d and the high concentration of F was 2700 microg/d. The moderate Ca concentration was 13 mg/d and the high Ca concentration was 260 mg/d. The moderate concentration of I was 3.5 microg/d and the low concentration of I was 0.23 microg/d. After twenty weeks, body weight was measured.
  
  
  RESULTS
  According to the results of factorial ANOVA, significant interaction effects of F with Ca were found (F = 5.933, P = 0.017). The empty body weight was measured at the end of the fifth month. When both iodine and fluorine were at the optimal level, the weight of group 2 [(262.5 +/- 47.1) g] and group 1 [(307.9 +/- 55.0) g] showed significant difference (t = 4.24, P < 0.05). When both iodine and fluorine were at low level, the weight of group 6 [(248.8 +/- 30.0) g] and group 5 [(293.3 +/- 19.7) g] showed significant difference (t = 4.16, P < 0.05). Animals with optimal iodine and calcium [(269.3 +/- 27.3) g] showed significant difference compared to the weight of low level iodine and optimal fluorine [(307.9 +/- 55.0) g]. When the low level iodine and optimal calcium were applied, weight of group 7 [(261.9 +/- 31.3) g] and group 5 [(293.3 +/- 19.7) g] showed significant difference. (t = 2.94, P < 0.05).
  
  
  CONCLUSIONS
  Interaction effects of F with Ca were found on body weight in rats.
authors.affiliation: Department of Preventive Medicine, Zunyi Medical College, Zunyi 563000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Xin
authors.initials: X
authors.lastname: Ling
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Dong
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D002136:Calcium, Dietary / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D005459:Fluorides; D005461:Fluorine / Q000494:pharmacology*; D007455:Iodine / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002136:Calcium, Dietary; D005461:Fluorine; D007455:Iodine; D005459:Fluorides