title: Alteration of diffusion tensor parameters in postmortem brain.
abstract: In autopsy of humans, there is usually an interval of hours to days between death and tissue fixation, during which the cadaver is stored below room temperature to retard tissue autolysis. We have attempted to model this process and evaluate the alteration in diffusion indices of the postmortem brain in pigs, which were kept at 4 degrees C. The pigs were scanned prior to death and at 3, 6, 9, 12, 18, 24, 30, 36, 42, 48 and 72 h postmortem. Regions of interest were placed in the corpus callosum, internal capsule, periventricular and subcortical white matter anteriorly and posteriorly. There was a slight increase in fractional anisotropy (FA) in the first 3 h postmortem. The FA remained stable up to 72 h postmortem. There was a marked decrease in trace, eigenmajor (lambda major), eigenmedium (lambda medium) and eigenminor (lambda minor), particularly in the first 3 h following death. This study supports the utility of measuring diffusion anisotropy if the time elapsed between death and tissue fixation is within 3 days. However, trace and eigenvalues decreased markedly within the first few hours postmortem. Therefore trace and eigenvalues obtained from ex vivo studies cannot be extrapolated to in vivo studies.
authors.affiliation: Diagnostic Imaging, Hospital for Sick Children, Toronto, Canada. Elysa.Widjaja@sickkids.ca
authors.forename: Elysa
authors.initials: E
authors.lastname: Widjaja
authors.forename: Xingchang
authors.initials: X
authors.lastname: Wei
authors.forename: Logi
authors.initials: L
authors.lastname: Vidarsson
authors.forename: Rahim
authors.initials: R
authors.lastname: Moineddin
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Macgowan
authors.forename: Daniel
authors.initials: D
authors.lastname: Nilsson
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D011180:Postmortem Changes; D012680:Sensitivity and Specificity; D013552:Swine