title: New insights into autophagic cell death in the gypsy moth Lymantria dispar: a proteomic approach.
abstract: Autophagy is an evolutionary ancient process based on the activity of genes conserved from yeast to metazoan taxa. Whereas its role as a mechanism to provide energy during cell starvation is commonly accepted, debate continues about the occurrence of autophagy as a means specifically activated to achieve cell death. The IPLB-LdFB insect cell line, derived from the larval fat body of the lepidoptera Lymantria dispar, represents a suitable model to address this question, as both autophagic and apoptotic cell death can be induced by various stimuli. Using morphological and functional approaches, we have observed that the culture medium conditioned by IPLB-LdFB cells committed to death by the ATPase inhibitor oligomycin A stimulates autophagic cell death in untreated IPLB-LdFB cells. Moreover, proteomic analysis of the conditioned media suggests that, in IPLB-LdFB cells, oligomycin A promotes a shift towards lipid metabolism, increases oxidative stress and specifically directs the cells towards autophagic activity.
authors.affiliation: Department of Animal Biology, University of Modena and Reggio Emilia, Via Campi 213/D, 41100, Modena, Italy. davide.malagoli@unimore.it
authors.forename: Davide
authors.initials: D
authors.lastname: Malagoli
authors.forename: Federica
authors.initials: F
authors.lastname: Boraldi
authors.forename: Giulia
authors.initials: G
authors.lastname: Annovi
authors.forename: Daniela
authors.initials: D
authors.lastname: Quaglino
authors.forename: Enzo
authors.initials: E
authors.lastname: Ottaviani
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001343:Autophagy / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D009036:Moths / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D040901:Proteomics
chemical_list: D017077:Culture Media, Conditioned; D000255:Adenosine Triphosphate