Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes.
The possibility of obtaining transplantable oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal failures. As reconstructive material this in vitro epithelia would be also useful for other parts of the human body. Many researchers still use controversial methods; therefore it was evaluated and compared the efficiency of the enzymatic and direct explant methods to obtain oral keratinocytes. To this project oral epithelia fragments were used. This work compared: time needed for cell obtainment, best cell amount, life-span and epithelia forming cell capacity. The results showed the possibility to obtain keratinocytes from a small oral fragment and we could verify the advantages and peculiar restrictions. We concluded that under our conditions the enzymatic method showed the best results: in the cells obtaining time needed, cell amount and life-span. Both methods showed the same capacity to form in vitro epithelia.
Klingbeil
Ma Fátima Guarizo
MF
Radiation Technology Center, Nuclear and Energetic Research Institute-IPEN/CNEN, Av. Lineu Prestes 2242, Cidade Universitária, São Paulo, Brazil. fakling@usp.br
Herson
Marisa Roma
MR
Cristo
Elier Broche
EB
dos Santos Pinto
Décio
D
Yoshito
Daniele
D
Mathor
Monica Beatriz
MB
D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology*; D009055:Mouth / Q000737:chemistry / Q000166:cytology*; D009061:Mouth Mucosa / Q000737:chemistry / Q000166:cytology*; D014357:Trypsin / Q000737:chemistry
D014357:Trypsin