title: Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis.
abstract: We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
authors.affiliation: Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Japan.
authors.forename: Kozue
authors.initials: K
authors.lastname: Oana
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D005821:Genetic Techniques; D016680:Genome, Bacterial; D009113:Muramidase / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009113:Muramidase