title: Prevalence of single and multiple human papillomavirus types in cervical cancer and precursor lesions in Hubei, China.
abstract: OBJECTIVE
  The objective of this study was to establish the prevalence and type distribution of human papillomavirus (HPV) in cervical cancer and cervical intraepithelial neoplasia (CIN) in Hubei, China.
  
  
  METHODS
  A total of 112 patients with cervical cancer and 60 CIN II-III samples were obtained from women undergoing biopsy or surgery. HPV and typing were examined by the polymerase chain reaction (PCR) and sequencing.
  
  
  RESULTS
  HPV DNA was detected in 105 patients with cervical cancer (93.75%), HPV 16 being detected in 91 (81.25%) cases, and HPV 58 in 7 (6.25%) cases. HPV DNA was detected in 50 patients with CIN II-III (83.33%), HPV 16 being detected in 37 (61.67%) cases, HPV 58 in 5 cases (8.33%). HPV 18 was not common in this study. About 11% cervical cancer and 8.33% CIN II-III specimens exhibited multiple infections (p = 0.79).
  
  
  CONCLUSIONS
  The high prevalence of HPV 16 in Hubei, China, deserves attention as it has important implications for the usefulness of vaccine and the choice of diagnostic methods.
authors.affiliation: Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, PR China. chb2105@163.com
authors.forename: Hong Bing
authors.initials: HB
authors.lastname: Cai
authors.forename: Xiao Hua
authors.initials: XH
authors.lastname: Ding
authors.forename: Chang Chun
authors.initials: CC
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D053918:Papillomavirus Vaccines / Q000276:immunology; D011230:Precancerous Conditions / Q000821:virology*; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral; D053918:Papillomavirus Vaccines