Acyl migration during deacylation of phospholipids rich in docosahexaenoic acid (DHA): an enzymatic approach for evidence and study.
Non-enzymatic acyl migration could be counter-productive for the preparation of structured phospholipids with docosahexaenoic acid (DHA) at a designated position. Therefore enzymatic approaches have been developed to investigate acyl migration. First, acyl migration from sn-2 to sn-1 position has been set into relief by a three step enzymatic method using a typo-selective lipase, a phospholipase A2 and a non-selective lipase. The effect of reaction temperature on acyl migration from sn-2 to sn-1 was monitored: lowering the reaction temperature from 40 to 30 degrees C allowed a reduction of DHA migration rate of 40%. Secondly, acyl migration from sn-1 to sn-2 position was negligible. This last result was obtained through the study of structured phosphatidylcholine selective deacylation using a phospholipase A2.
Poisson
Laurent
L
Mer, Molécules, Santé-EA 2160, Université de Nantes, 44072, Nantes cedex 03, France. laurent.poisson@univ-lemans.fr
Devos
Marie
M
Godet
Stéphanie
S
Ergan
Françoise
F
Pencreac'h
Gaëlle
G
D004281:Docosahexaenoic Acids / Q000378:metabolism*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D013696:Temperature
D010743:Phospholipids; D004281:Docosahexaenoic Acids; D008049:Lipase