title: Understanding enzyme immobilisation.
abstract: Enzymes are versatile catalysts in the laboratory and on an industrial scale. To broaden their applicability in the laboratory and to ensure their (re)use in manufacturing the stability of enzymes can often require improvement. Immobilisation can address the issue of enzymatic instability. Immobilisation can also help to enable the employment of enzymes in different solvents, at extremes of pH and temperature and exceptionally high substrate concentrations. At the same time substrate-specificity, enantioselectivity and reactivity can be modified. However, most often the molecular and physical-chemical bases of these phenomena have not been elucidated yet. This tutorial review focuses on the understanding of enzyme immobilisation.
authors.affiliation: Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, Delft, 2628 BL, The Netherlands. u.hanefeld@tudelft.nl
authors.forename: Ulf
authors.initials: U
authors.lastname: Hanefeld
authors.forename: Lucia
authors.initials: L
authors.lastname: Gardossi
authors.forename: Edmond
authors.initials: E
authors.lastname: Magner
mesh_terms: D000327:Adsorption; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D007477:Ions / Q000737:chemistry; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D004800:Enzymes, Immobilized; D007477:Ions; D008049:Lipase