title: Expression profiles of SV40-immortalization-associated genes upregulated in various human cancers.
abstract: Immortalization is an early and essential step of human carcinogenesis which is associated with alterations in gene expression and regulation. Suppression subtractive hybridization (SSH) was successfully performed to identify immortalization-associated genes upregulated in SV40-immortalized lung fibroblasts. We identified 116 known genes which were related to diverse functions, with 32.8% relevant for cell cycle or proliferation indicating the potential involvement of these genes in immortalization. We chose eight known genes located on the overrepresented chromosomes of non-small-cell lung cancers (NSCLCs). ASPM, RFC4, C3orf26, BXDC2, C15orf44, AURKA, C20orf77, and RBMX were upregulated in immortalized cells, cancer cells, and non-small-cell lung cancer (NSCLC) tissues. We additionally cloned two novel genes (CHA-V-97 and CHA-V-165) which showed similar upregulated expression patterns in cells and tissues examined. Identification and further characterization of these genes may provide insights of novel players for immortalization and human carcinogenesis.
authors.affiliation: Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Seongnam, South Korea.
authors.forename: Hyun Min
authors.initials: HM
authors.lastname: Jung
authors.forename: Seong-Jun
authors.initials: SJ
authors.lastname: Choi
authors.forename: Jin Kyeoung
authors.initials: JK
authors.lastname: Kim
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics*; D005347:Fibroblasts; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D013539:Simian virus 40; D015854:Up-Regulation