Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.
Gil
Jung-Eun
JE
Laboratory of Stem Cell Biology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Woo
Dong-Hun
DH
Shim
Joong-Hyun
JH
Kim
Sung-Eun
SE
You
Hyun-Ju
HJ
Park
Sung-Hye
SH
Paek
Sun Ha
SH
Kim
Suel-Kee
SK
Kim
Jong-Hoon
JH
D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D055495:Neurogenesis; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D019096:Vitronectin / Q000378:metabolism*; D000099176:Noggin Protein
D015415:Biomarkers; D002352:Carrier Proteins; D019096:Vitronectin; D000099176:Noggin Protein