title: Seroprevalence of avian reovirus in egg-laying chicken flocks in China.
abstract: In the present study, the epidemiologic status of avian reovirus (ARV) infections was investigated from egg-laying chicken flocks in China with an enzyme-linked immunosorbent assay (ELISA) kit. Because the chickens were not vaccinated against ARV, antibodies were attributed to the infection. Antibodies specific to ARV were found in more than 92% (542/587) of the average positivity and ranged from 30% to 100% in different chicken population. A virus, designated HB06, was isolated from flocks with suspicious ARV infections. Sequence analysis of the S1 gene revealed that strain HB06 was closely related with the most ARVs with less than 2% nucleotide divergence, and the homology was highest with the vaccine strain S1133, with a 98.97% nucleotide identity. The potential significance of vaccination against ARV in egg-laying chicken flocks in China is also discussed.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Pu
authors.forename: Xingli
authors.initials: X
authors.lastname: Liu
authors.forename: Youchun
authors.initials: Y
authors.lastname: Guo
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhao
authors.forename: Guozhong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002645:Chickens; D002681:China / Q000453:epidemiology; D018076:DNA, Complementary / Q000737:chemistry; D008969:Molecular Sequence Data; D030101:Orthoreovirus, Avian / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010058:Oviposition; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology*; D012367:RNA, Viral / Q000235:genetics; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D018076:DNA, Complementary; D012367:RNA, Viral; C031728:sigma 1 protein, reovirus