[Establishment of human embryonic stem cell line NJGLLhES1].
OBJECTIVE
To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1.


METHODS
From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1. We detected the karyotype of the cell line, determined the expressions of alkaline phosphatase, the specific cell surface antigens SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and the marker gene Oct-4, and examined the formation of embryoids and teratomas.


RESULTS
NJGLLhES1 was maintained for over 1 year in vitro, with the morphological characteristics of hESC, a normal karyotype, positive expressions of alkaline phosphatase and specific cell marker genes, and the potential of forming embryoids and teratomas.


CONCLUSIONS
A new human embryonic stem cell line NJGLLhES1 was successfully established, which remains karyotypically and phenotypically stable, undifferentiated and capable of self-renewal and pluripotential differentiation.
Sun
Hai-Xiang
HX
Center of Reproductive Medicine, Drum Tower Hospital Affiliated to Nanjing University School of Medicine, Nanjing, Jiangsu 210008, China. stevensunz@163.com
Zhao
Xia
X
Hu
Ya-Li
YL
Zhang
Ning-Yuan
NY
Wang
Bin
B
Chen
Hua
H
Wang
Jun-Xia
JX
Xu
Zhi-Peng
ZP
Chen
Lin-Jun
LJ
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR