[Studies on chemical constituents of Ranunculus japonicus].
OBJECTIVE
To isolate and determine the chemical constituents of Ranunculus japonicus in Liupan Mountain, Ningxia province, China.


METHODS
The herb was extracted with ethanol by ultrasonic bath. The extractives were divided to petroleum ether, ethyl acetate, n-butanol parts. The first two parts were separated and purified with silica gel and Sephadex LH -20 column chromatography. The structures of the separated compounds were idnetefied by physical and chemical properties and spectral analysis.


RESULTS
Nine compounds were isolated and identified as follows: scoparone (1), tricin (2), protocatechuic acid (3), luteolin (4), anemonin (5), scopoletin (6), 5-hydroxy-6, 7-dimethoxyflavone (7), ternatolide (8), 5-hydroxy-7, 8-dimethoxy-flavone (9).


CONCLUSIONS
Compounds 1-9 were isolated from Ranunculus japonicus for the first time.
Liang
Yong-Feng
YF
College of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, China. qylyf338@yahoo.com.cn
Chen
Zuo-Tao
ZT
Liu
Li-Hong
LH
D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000473:Alkanes / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry; D005663:Furans / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry; D047311:Luteolin / Q000737:chemistry; D031947:Ranunculus / Q000737:chemistry*; D012603:Scopoletin / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D000085:Acetates; D000473:Alkanes; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D005663:Furans; D062385:Hydroxybenzoates; C009091:protocatechuic acid; D000431:Ethanol; C007650:ethyl acetate; D020001:1-Butanol; C017769:tricin; C028304:anemonin; C018145:scoparone; D012603:Scopoletin; D047311:Luteolin; C004544:naphtha; C043562:flavone