title: Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate.
abstract: In the present study, we tried to establish an efficient assay for total antioxidant capacity (TAC) in human plasma using a 96-well microplate. TAC was assessed using lag time by antioxidants against the myoglobin-induced oxidation of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) with hydrogen peroxide, and expressed as Trolox equivalent. The linearity of the calibration curve with Trolox was maintained with the Trolox concentration range from 2.5 microM to 25 microM (R(2) = 0.997). The assay was applied to the measurement of TAC in healthy human plasma. Coefficient of variation in intraday assay was 2.4%. Difference was not observed in interday assay. Plasma TAC of men ((569 +/- 41) microM Trolox equivalent; n = 6) was higher than that of women ((430 +/- 28) microM Trolox equivalent; n = 4). After the vegetable juice was drunk for 1 week, the increase in plasma TAC was observed in almost all the volunteers. In summary, we developed the efficient assay for plasma TAC using a 96-well microplate.
authors.affiliation: Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kambayashi
authors.forename: Nguyen Thanh
authors.initials: NT
authors.lastname: Binh
authors.forename: Hiroki
authors.initials: H
authors.lastname: W Asakura
authors.forename: Yuri
authors.initials: Y
authors.lastname: Hibino
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Hitomi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino