title: The natural xanthone alpha-mangostin reduces oxidative damage in rat brain tissue.
abstract: The antiperoxidative properties of alpha-mangostin, a xanthone isolated from mangosteen fruit, were tested for the first time in nerve tissue exposed to different toxic insults. Two reliable biological preparations (rat brain homogenates and synaptosomal P2 fractions) were exposed to the toxic actions of a free radical generator (ferrous sulfate), an excitotoxic agent (quinolinate), and a mitochondrial toxin (3-nitropropionate). alpha-Mangostin decreased the lipoperoxidative action of FeSO(4) in both preparations in a concentration-dependent manner, and completely abolished the peroxidative effects of quinolinate, 3-nitropropionate and FeSO(4) + quinolinate at all concentrations tested. Interestingly, when tested alone in brain homogenates, alpha-mangostin significantly decreased the lipoperoxidation even below basal levels. alpha-Mangostin also prevented the decreased reductant capacity of mitochondria in synaptosomal fractions. Our results suggest that alpha-mangostin exerts a robust antiperoxidative effect in brain tissue preparations probably through its properties as a free radical scavenger. In light of these findings, this antioxidant should be tested in other neurotoxic models involving oxidative stress.
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur México DF, Mexico.
authors.forename: Berenice
authors.initials: B
authors.lastname: Márquez-Valadez
authors.forename: Rafael
authors.initials: R
authors.lastname: Lugo-Huitrón
authors.forename: Verónica
authors.initials: V
authors.lastname: Valdivia-Cerda
authors.forename: Luis Rubén
authors.initials: LR
authors.lastname: Miranda-Ramírez
authors.forename: Verónica
authors.initials: V
authors.lastname: Pérez-De La Cruz
authors.forename: Octavio
authors.initials: O
authors.lastname: González-Cuahutencos
authors.forename: Isabel
authors.initials: I
authors.lastname: Rivero-Cruz
authors.forename: Rachel
authors.initials: R
authors.lastname: Mata
authors.forename: Abel
authors.initials: A
authors.lastname: Santamaría
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverrí
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D009574:Nitro Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D011422:Propionates / Q000037:antagonists & inhibitors / Q000494:pharmacology; D017378:Quinolinic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; D009574:Nitro Compounds; D011422:Propionates; D017392:Thiobarbituric Acid Reactive Substances; D044004:Xanthones; C020748:ferrous sulfate; D017378:Quinolinic Acid; C015392:3-nitropropionic acid; C021053:mangostin