title: Quantitative radiographic evaluation of periapical bone resorption in dog's teeth contaminated with bacterial endotoxin (LPS) associated or not with calcium hydroxide.
abstract: The aim of this study was to quantify radiographically the periapical bone resorption in dogs' teeth contaminated with bacterial endotoxin (LPS), associated or not with calcium hydroxide. After pulp tissue removal, 60 premolars were randomly assigned to 4 groups and were either filled with LPS (group 1), filled with LPS plus calcium hydroxide (group 2) or filled with saline (group 3) for a period of 30 days. In group 4, periapical lesion formation was induced with no canal treatment. Standardized radiographs were taken at the beginning of the treatment and after 30 days and the Image J Program was used for measurement of periapical lesion size. Periapical lesions were observed in groups 1 (average of 8.44 mm2) and 4 (average of 3.02 mm2). The lamina dura was intact and there were no areas of periapical bone resorption in groups 2 and 3. It may be concluded that calcium hydroxide was effective in inactivating LPS, as demonstrated by the absence of apical periodontitis in the roots that were filled with bacterial endotoxin plus calcium hydroxide.
authors.affiliation: Department of Pediatric, Preventive and Community Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Léa Assed Bezerra
authors.initials: LA
authors.lastname: da Silva
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: da Silva
authors.forename: Luis Guilherme Siqueira
authors.initials: LG
authors.lastname: Branco
authors.forename: Valéria Pontelli
authors.initials: VP
authors.lastname: Navarro
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000517:prevention & control*; D000818:Animals; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D003786:Dental Pulp Cavity / Q000000981:diagnostic imaging / Q000382:microbiology; D004285:Dogs; D004926:Escherichia coli; D008070:Lipopolysaccharides; D010485:Periapical Periodontitis / Q000000981:diagnostic imaging / Q000517:prevention & control*; D011859:Radiography; D011897:Random Allocation; D012387:Root Canal Filling Materials / Q000627:therapeutic use*
chemical_list: D008070:Lipopolysaccharides; D012387:Root Canal Filling Materials; D002126:Calcium Hydroxide