title: New flavonoid glycosides and cyanogenic glycosides from Dracocephalum peregrinum.
abstract: Separation of ethyl acetate fractionation of Dracocephalum peregrinum afforded three new flavonoid glucosides (1-3), and a new cyanogenic glucoside (4). Their structures were elucidated based on HR-electron spray ionization (ESI)-MS, EI-MS, UV, IR, 1D-, and 2D-NMR data. 1-4 were tested in vitro for their antiinflammatory activity against the RAW 264.7, 293 cells. Among the compounds tested, 1-4 shown good antiinflammatory activity at 100 mug/ml by the measurement of nitric oxide (NO) in lipopolysaccharide (LPS) activated macrophages. But only 2 and 3 shown weak antiinflammatory activity at 100 mug/ml during the nuclear factor (NF)-kappaB activation assay.
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fu
authors.forename: Chun-Chao
authors.initials: CC
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.forename: Xi-Ke
authors.initials: XK
authors.lastname: Xu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002460:Cell Line; D004121:Dimethyl Sulfoxide; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D006027:Glycosides; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012997:Solvents; D009569:Nitric Oxide; D004121:Dimethyl Sulfoxide