title: Layer-by-layer technique as a new approach to produce nanostructured films containing phospholipids as transducers in sensing applications.
abstract: Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers onto Pt interdigitated electrodes forming sensing units of an electronic tongue system. Low concentrations (nM level) of a phenothiazine compound were detected through impedance spectroscopy. Both negative 1,2-dipalmitoyl-sn-3-glycero-[phosphor-rac-(1-glycerol)] (DPPG) and zwitterionic l-alpha-1,2-dipalmitoyl-sn-3-glycero-phosphatidylcholine (DPPC) phospholipids were used to produce the LbL films, whose molecular architecture was monitored combining spectroscopy and microscopy at micro and nanoscales. The sensor array was complemented by Langmuir-Blodgett (LB) monolayers of DPPG and DPPC deposited onto Pt interdigitated electrodes as well. It was found that the distinct molecular architecture presented by both LbL and LB films plays a key role on the sensitivity of the sensor array with the importance of the LbL films being demonstrated by principal component analysis (PCA).
authors.affiliation: Faculdade de Ciencias e Tecnologia, UNESP, Presidente Prudente/SP, 19060-900 Brazil.
authors.forename: P H B
authors.initials: PH
authors.lastname: Aoki
authors.forename: D
authors.initials: D
authors.lastname: Volpati
authors.forename: A
authors.initials: A
authors.lastname: Riul
authors.forename: W
authors.initials: W
authors.lastname: Caetano
authors.forename: C J L
authors.initials: CJ
authors.lastname: Constantino
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D004364:Pharmaceutical Preparations; D010743:Phospholipids / Q000737:chemistry*; D013053:Spectrophotometry; D014159:Transducers
chemical_list: D004364:Pharmaceutical Preparations; D010743:Phospholipids