Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats.
OBJECTIVE
Liver ischemia-reperfusion injury is a phenomenon presents in events like liver resections and transplantation. The restoration of blood flow may leads to local and systemic injury. Several techniques have been developed in order to avoid or ameliorate ischemia-reperfusion injury in clinical situations. The application of a stutter reperfusion after the ischemic event (postconditioning) could alters the hydrodynamics and stimulates endogenous mechanisms that attenuate the reperfusion injury. The present study was designed to evaluate the potential protective effect of postconditioning in a model of ischemia-reperfusion in rats.


METHODS
Hepatic anterior pedicle of median and left anterolateral segments were exposed and clamped for 1 hour. Two hours later, clamp was released in two different ways: Control Group (n=7): clamp was release straightforward; Postconditioning Group (n=7): clamp was released intermittently. Lipid peroxidation (malondialdehyde) and expression of the glutathione-s-transferase-alpha-3 gene were studied.


RESULTS
Lipid peroxidation was significantly decreased in ischemic and non-ischemic liver by postconditioning. GST- alpha3 gene was overexpressed in post-conditioned group, but not significantly.


CONCLUSIONS
Postconditioning induced hepatoprotection by reducing lipid peroxidation in the ischemic and non-ischemic liver.
Teixeira
Antonio Roberto Franchi
AR
Liver Transplantation and Surgery Unit, Department of Gastroenterology, Faculty of Medicine, University of Sao Paulo, Brazil. drrteixeira@uol.com.br
Molan
Nilza T
NT
Kubrusly
Márcia Saldanha
MS
Bellodi-Privato
Marta
M
Coelho
Ana Maria
AM
Leite
Kátia R
KR
Machado
Marcel Autran Cesar
MA
Bacchella
Telésforo
T
Machado
Marcel Cerqueira César
MC
D000818:Animals; D015415:Biomarkers / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood / Q000235:genetics; D007511:Ischemia / Q000517:prevention & control*; D019194:Ischemic Preconditioning; D007527:Isoenzymes / Q000097:blood / Q000235:genetics; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000098:blood supply*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
D015415:Biomarkers; D007527:Isoenzymes; D008315:Malondialdehyde; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha