title: Novel mucoadhesive nasal inserts based on chitosan/hyaluronate polyelectrolyte complexes for peptide and protein delivery.
abstract: OBJECTIVE
  The purpose of this study was the preparation and characterisation of mucoadhesive nasal inserts based on chitosan/hyaluronate polyelectrolyte complexes prepared at various pHs and at different molar ratios.
  
  
  METHODS
  A suspension of chitosan/hyaluronate complexes with or without the model drugs (vancomycin or insulin) was lyophilised into small inserts. Complexation yield, FT-IR spectra and thermogravimetric analysis were used to study the degree of interactive strength between polyions. In-vitro swelling, mucoadhesion and release tests were performed in order to investigate delivery of vancomycin and insulin in the nasal cavity.
  
  
  RESULTS
  The results indicated that the selection of complex preparative conditions allows modulation of insert swelling and mucoadhesion ability. Nasal inserts containing vancomycin or insulin had showed completely different drug release behaviour.
  
  
  CONCLUSIONS
  Chitosan/hyaluronate polyelectrolyte complexes can be used for the formulation of mucoadhesive nasal inserts for the delivery of peptide and protein drugs.
authors.affiliation: Department of Pharmaceutical Sciences, Bologna University, Bologna, Italy. barbara.luppi@unibo.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Luppi
authors.forename: Federica
authors.initials: F
authors.lastname: Bigucci
authors.forename: Laura
authors.initials: L
authors.lastname: Mercolini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Musenga
authors.forename: Milena
authors.initials: M
authors.lastname: Sorrenti
authors.forename: Laura
authors.initials: L
authors.lastname: Catenacci
authors.forename: Vittorio
authors.initials: V
authors.lastname: Zecchi
mesh_terms: D000269:Adhesives / Q000737:chemistry / Q000378:metabolism*; D000281:Administration, Intranasal; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000493:pharmacokinetics; D009297:Nasal Mucosa / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011506:Proteins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014640:Vancomycin / Q000493:pharmacokinetics; D014783:Viscosity; D014867:Water / Q000737:chemistry / Q000378:metabolism
chemical_list: D000269:Adhesives; D007328:Insulin; D010455:Peptides; D011506:Proteins; D014867:Water; D014640:Vancomycin; D006820:Hyaluronic Acid; D048271:Chitosan