Proteomic analysis of plasma membrane vesicles.
A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flowcell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived.
Bauer
Brigitte
B
Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296 Göteborg, Sweden.
Davidson
Max
M
Orwar
Owe
O
D002462:Cell Membrane / Q000737:chemistry*; D002853:Chromatography, Liquid; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D044085:Microfluidics / Q000379:methods; D010455:Peptides / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D008565:Membrane Proteins; D010455:Peptides