title: The Panton-Valentine leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus aureus USA300.
abstract: Methicillin-resistant Staphylococcus aureus is increasingly responsible for staphylococcal infections in the community. A large percentage of the community-acquired methicillin-resistant (CA-MRSA) strains in the USA produce Panton-Valentine leukocidin (PVL), which is associated with severe infections. The virulence of the clinical CA-MRSA strain USA300 was compared to that of its isogenic pvl-deleted mutant, and it was shown that PVL contributes to lung and muscle tissue destruction, respectively, in murine necrotizing pneumonia and skin infection models. Mice infected with the USA300 strain developed a dominant anti-PVL response. The PVL subunits were therefore tested as vaccinogens against this isolate, and their vaccine efficacy correlated with both the route of vaccination and infection. These data suggest that PVL is a virulence factor in murine CA-MRSA infections.
authors.affiliation: University of Texas School of Public Health, Houston, TX, USA.
authors.forename: E L
authors.initials: EL
authors.lastname: Brown
authors.forename: O
authors.initials: O
authors.lastname: Dumitrescu
authors.forename: D
authors.initials: D
authors.lastname: Thomas
authors.forename: C
authors.initials: C
authors.lastname: Badiou
authors.forename: E M
authors.initials: EM
authors.lastname: Koers
authors.forename: P
authors.initials: P
authors.lastname: Choudhury
authors.forename: V
authors.initials: V
authors.lastname: Vazquez
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: G
authors.initials: G
authors.lastname: Lina
authors.forename: F
authors.initials: F
authors.lastname: Vandenesch
authors.forename: M G
authors.initials: MG
authors.lastname: Bowden
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology*; D001835:Body Weight; D005098:Exotoxins / Q000276:immunology* / Q000502:physiology; D005260:Female; D007956:Leukocidins / Q000276:immunology* / Q000502:physiology; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018410:Pneumonia, Bacterial / Q000517:prevention & control*; D012867:Skin / Q000473:pathology; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013207:Staphylococcal Skin Infections / Q000517:prevention & control*; D013209:Staphylococcal Vaccines / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity; D016019:Survival Analysis; D014774:Virulence; D037521:Virulence Factors / Q000276:immunology* / Q000502:physiology
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D013209:Staphylococcal Vaccines; D037521:Virulence Factors