Fiber-optic endomicroscopy system for high-resolution nonlinear imaging of biological tissue.
A flexible fiber-optic endomicroscopy system was developed for nonlinear optical imaging of biological samples. Double-clad fiber and photonic bandgap fiber were employed in the endomicroscope for femtosecond pulse delivery, dispersion compensation, nonlinear optical signals collection and fast beam scanning. Three-dimensional imaging of biological tissues based on second harmonic generation and two-photon excited fluorescence was performed with the endomicroscope. The depth-resolved imaging results strongly suggest the potential of this flexible fiber-optic microscope system for real-time assessment of both epithelial and stromal structures in luminal organs.
Wu
Yicong
Y
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Xi
Jiefeng
J
Cobb
Michael J
MJ
Li
Xingde
X
D000818:Animals; D001698:Biomedical Engineering; D019723:Endoscopes / Q000706:statistics & numerical data; D021621:Imaging, Three-Dimensional; D066298:In Vitro Techniques; D008853:Microscopy / Q000295:instrumentation* / Q000706:statistics & numerical data; D017711:Nonlinear Dynamics; D055100:Optical Fibers / Q000706:statistics & numerical data; D051381:Rats; D012815:Signal Processing, Computer-Assisted; D013552:Swine