[Grape seed extract induces morphological changes of prostate cancer PC-3 cells].
OBJECTIVE
To observe the morphological changes of prostate cancer PC-3 cells induced by grape seed extract (GSE).


METHODS
PC-3 cells were incubated with different concentrations of GSE (100, 200 and 300 microg/ml) for 24, 48 and 72 hours, and then observed for morphological changes by invert microscopy, HE staining and transmission electron microscopy.


RESULTS
The incubated PC-3 cells appeared round, small, wrinkled and broken under the invert microscope and exhibited the classical morphological characteristics of cell death under the electron microscope, including cell atrophy, increased vacuoles, crumpled nuclear membrane, and chromosome aggregation.


CONCLUSIONS
GSE can cause morphological changes and induce necrosis and apoptosis of PC-3 cells.
Shang
Xue-Jun
XJ
Department of Andrology, School of Clinical Medicine, Nanjing University Medical College/Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. shangxj@androl.cn
Yin
Hong-Lin
HL
Ge
Jing-Ping
JP
Sun
Yi
Y
Teng
Wen-Hui
WH
Huang
Yu-Feng
YF
D017209:Apoptosis; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy; D027843:Vitis
D010936:Plant Extracts