title: A primary role for Golgi positioning in directed secretion, cell polarity, and wound healing.
abstract: Peri-centrosomal positioning of the mammalian Golgi apparatus is known to involve microtubule-based motility, but its importance for cellular physiology is a major unanswered question. Here, we identify golgin-160 and GMAP210 as proteins required for centripetal motility of Golgi membranes. In the absence of either golgin, peri-centrosomal positioning of the Golgi apparatus was disrupted while the cytoskeleton remained intact. Although secretion persisted with normal kinetics, it was evenly distributed in response to wounding rather than directed to the wound edge. Strikingly, these cells also completely failed to polarize. Further, directionally persistent cell migration was inhibited such that wound closure was impaired. These findings not only reveal novel roles for golgin-160 and GMAP210 in conferring membrane motility but also indicate that Golgi positioning has an active role in directed secretion, cell polarity, and wound healing.
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
authors.forename: Smita
authors.initials: S
authors.lastname: Yadav
authors.forename: Sapna
authors.initials: S
authors.lastname: Puri
authors.forename: Adam D
authors.initials: AD
authors.lastname: Linstedt
mesh_terms: D000199:Actins / Q000378:metabolism; D016764:Cell Polarity; D003599:Cytoskeleton / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D014945:Wound Healing
chemical_list: D000199:Actins; D034741:RNA, Small Interfering