Utilization of the porcine system to study lymphotoxin-beta regulation in intestinal lymphoid tissue.
Lymphotoxin-beta (LT-beta) has been suggested to be a regulator of secondary lymphoid structure development. In the present study, we isolated porcine LT-beta (poLT-beta) from adult swine spleens. The open reading frame encoded a predicted 246-amino acid polypeptide exhibiting higher similarity to the human than the mouse LT-beta protein. Expression of LT-beta mRNA in various swine tissues was analyzed by real-time PCR, and it was found to be higher in the ileal Peyer's patches (Pps) of adults than in newborns. In addition, ligand stimulation of toll-like receptors 2, 4, and 9, which are activated by bacterial components, increased LT-beta expression only in neonatal ileal Pps. These results suggest that colonization by commensal bacteria may affect the maturation of neonatal ileal Pps by the induction of LT-beta via toll-like receptors. LT-beta may therefore be useful for studying the development of the intestinal immune system at parturition in both swine and humans.
Shimazu
Tomoyuki
T
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan.
Tohno
Masanori
M
Katoh
Shinichiro
S
Shimosato
Takeshi
T
Aso
Hisashi
H
Kawai
Yasushi
Y
Saito
Tadao
T
Kitazawa
Haruki
H
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D007413:Intestinal Mucosa / Q000276:immunology*; D053308:Lymphotoxin-beta / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010581:Peyer's Patches / Q000276:immunology; D016415:Sequence Alignment; D034421:Sus scrofa; D051193:Toll-Like Receptors / Q000276:immunology; D015854:Up-Regulation
D053308:Lymphotoxin-beta; D051193:Toll-Like Receptors