title: Reversible whole-organism cell cycle arrest in a living vertebrate.
abstract: In vivo cell cycle analysis in higher eukaryotes has been limited by the challenge of preserving the integrity of the living organism while visualizing dividing cells. Here, we propose a new model, which uses the unique combination of features of the Japanese medaka in order to visualize and manipulate the cell cycle progression in a live vertebrate. Our stable transgenic histone H2B-GFP medaka line allows fluorescence-based monitoring of the chromosomes. The system has a high specificity, with a strong GFP signal labeling the chromatin architecture. The subcellular resolution ensures detection of both normal and abnormal divisions in live recordings. This translates into the possibility to quantify temporal and spatial aspects of the cell cycle, such as length or nuclear size, as well as to expose drug toxicity at the earliest stage. We also show that acclimation to cold, a prominent feature of the eurytherm medaka, is a valuable natural way of inducing a reversible cell cycle arrest in the entire living organism. Our results suggest that this manipulation can be performed from the early stages of development, has no toxicity and does not alter the cell cycle profile of the embryo.
authors.affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Oltea
authors.initials: O
authors.lastname: Sampetrean
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Iida
authors.forename: Shinji
authors.initials: S
authors.lastname: Makino
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: Kikuo
authors.initials: K
authors.lastname: Ohno
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Saya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D002453:Cell Cycle / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009990:Oryzias / Q000033:anatomy & histology / Q000196:embryology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D006657:Histones; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins