title: Revision of the absolute configuration at C(23) of lanostanoids and isolation of secondary metabolites from formosan soft coral Nephthea erecta.
abstract: Three new oxygenated ergostanoids, 1-3, one known ergostanoid, 4, one new trinoreudesmadienone, 5, one new calamenene type sesquiterpene, 6, and one known aristolane-type sesquiterpene, (-)-aristolone (7), have been isolated from the AcOEt extract of the soft coral Nephthea erecta. The structures of these compounds were determined by extensive spectroscopic and X-ray crystallographic analysis, as well as Mosher's method. We revised the absolute configuration at C(23) in the side chain of some lanostanoids as a result of the Mosher's products of 1 (i.e., 1a and 1b). The cytotoxicities against selected cancer cells and the anti-inflammatory effects of these tested metabolites 1-7 were determined in vitro.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Shi-Yie
authors.initials: SY
authors.lastname: Cheng
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.forename: Shang-Kewi
authors.initials: SK
authors.lastname: Wang
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Chiang
authors.forename: Ali A H
authors.initials: AA
authors.lastname: El-Gamal
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.forename: Chang-Yih
authors.initials: CY
authors.lastname: Duh
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002460:Cell Line; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice
chemical_list: C497083:lanostanoid; D007810:Lanosterol