Yeast heat-shock protein gene HSP26 enhances freezing tolerance in Arabidopsis.
In the yeast Saccharomyces cerevisiae, the molecular chaperone Hsp26 is one component of the heat-shock response. Hsp26 has the remarkable ability to directly sense increases in temperature and switch from an inactive state to a chaperone-active state. In this study, we report a functional analysis of Hsp26 in Arabidopsis thaliana and its response to freezing stress. After freezing stress, the HSP26 transgenic plants exhibited stronger growth than the wild-type plants. We found that over-expression of HSP26 in Arabidopsis increased the amounts of free proline and soluble sugars, elevated the expression of stress defense genes, and enhanced Arabidopsis tolerance to freezing stress. Taken together, our results indicate that Hsp26 may play an important role in the response of transgenic Arabidopsis plants to freezing stresses.
Xue
Yong
Y
Shanghai Key Laboratory of Agricultural Genetic Breeding, Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. yonxue@gmail.com
Peng
Rihe
R
Xiong
Aisheng
A
Li
Xian
X
Zha
Dingshi
D
Yao
Quanhong
Q
D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D050260:Carbohydrate Metabolism; D004553:Electric Conductivity; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D012995:Solubility; D013312:Stress, Physiological / Q000235:genetics; D014158:Transcription, Genetic
C499528:HSP26 protein, S cerevisiae; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D008315:Malondialdehyde; D011392:Proline