Antioxidant activity of methanolic extract of Bombax ceiba.
The antioxidant activity of a methanolic extract of Bombax ceiba was evaluated using several antioxidant assays, in terms of its: (i) ability to scavenge DPPH (1,1-diphenyl-2-picryl-hydrazyl) and hydroxyl free radicals; (ii) action against lipid peroxidation (in rat liver microsomes and soy bean phosphatidylcholine liposomes), induced by ascorbyl radicals and peroxynitrite; and (iii) effect on myeloperoxidase activity. The cytotoxicity was monitored through the mitochondrial activity in the Vero cell line. The extract showed antioxidant activity in all assays, the EC(50) (microg/ml) for DPPH was 87 and for lipid peroxidation of microsomes and soy bean liposomes induced by ascorbyl radicals were 141 and 105, respectively, and by peroxynitrite were 115 and 77, respectively. The K(0.5) value for myeloperoxidase activity inhibition by the extract was 264 microg/ml. The extract showed very low toxicity toward Vero cells.
Vieira
Tiago O
TO
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
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Ataa
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Aboutabl
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Mona
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Creczynski-Pasa
Tânia B
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D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D035163:Bombax / Q000737:chemistry*; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D006834:Hydrazines / Q000737:chemistry; D017665:Hydroxyl Radical / Q000378:metabolism; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000201:enzymology; D000432:Methanol / Q000737:chemistry; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D014709:Vero Cells
D000975:Antioxidants; D001713:Biphenyl Compounds; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D009195:Peroxidase; D000432:Methanol