Experimental study of the bone marrow protective effect of a traditional Chinese compound preparation.
This study investigated the effect of Wei Gan Li on bone marrow haemopoiesis in myelosuppressed anaemic mice induced by (60)Cogamma, cyclophosphamide and chloramphenicol. After treatment with Wei Gan Li, colony forming units of granulocyte macrophages, erythroid cells, burst forming unit-erythroid cells, megakaryocytes and the proliferation of bone marrow stromal cells were measured by in vitro cell culture techniques. Bone marrow haemopoietic stem cells were measured by flow cytometry. The peripheral blood cell count was found to have significantly recovered after Wei Gan Li administration and the proliferation of bone marrow haemopoietic stem/progenitor cells and BMSC were also significantly increased. Wei Gan Li was therefore found to promote the recovery of bone marrow haemopoietic function in myelosuppressed anaemic mice.
Chen
Yongfeng
Y
Department of Anatomy, Taizhou University School of Medicine, Taizhou 318000, Zhejiang, China. chenyongfeng2006@yahoo.com.cn
Zhu
Bide
B
Zhang
Li
L
Yan
Suchun
S
Li
Jing
J
D000740:Anemia / Q000139:chemically induced; D000818:Animals; D001772:Blood Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000187:drug effects*; D005434:Flow Cytometry; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
D004365:Drugs, Chinese Herbal; C540476:weiganli