title: Local application of tetracycline solution with a microbrush: an alternative treatment for persistent periodontitis.
abstract: OBJECTIVE
  Topical antibiotics may overcome shortcomings of mechanical therapy for localized persistent periodontitis. This double-center, single-blind, randomized controlled study aimed to evaluate the microbiologic profile and clinical parameters of persistent periodontal pockets after treatment with tetracycline solution.
  
  
  METHODS
  Thirty-seven patients who had at least 4 non-adjacent sites of persistent periodontal pockets with probing depth of at least 5 mm and bleeding on probing were randomly assigned to test and control groups. In the test group, 2 teeth received 4 applications of tetracycline solution (100 mg/mL) with a microbrush(T), while the other 2 teeth received the same treatment plus 1 session of scaling and root planing(SRP+T). In the control group, 2 teeth received 1 session of scaling and root planing(SRP), and the other 2 teeth received 4 applications of saline with a microbrush plus 1 session of scaling and root planing(SRP+S). Clinical parameters of probing depth, bleeding on probing, visible plaque index, gingival bleeding index, gingival recession, as well as clinical attachment level and subgingival plaque samples (evaluated by polymerase chain reaction) were measured at baseline and 1, 3, and 6 months.
  
  
  RESULTS
  All therapies yielded statistically significant data on clinical measurements with no significant differences among groups. Presence of bacteria decreased in both groups, but only in the test group was a significant decrease of Porphyromonasgingivalis,Tannerellaforsythia, and Actinobacillusactinomycetemcomitans noted up to 6 months.
  
  
  CONCLUSIONS
  Tetracycline applied with a microbrush may be an alternative treatment for persistent periodontitis that can probably be mediated by reduction of microorganism proliferation
authors.forename: Joseane M D
authors.initials: JMD
authors.lastname: Bosco
authors.forename: Beatriz M V
authors.initials: BMV
authors.lastname: Lopes
authors.forename: Alvaro F
authors.initials: AF
authors.lastname: Bosco
authors.forename: Denise M P
authors.initials: DMP
authors.lastname: Spolidorio
authors.forename: Rosemary A C
authors.initials: RAC
authors.lastname: Marcantonio
mesh_terms: D000287:Administration, Topical; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001439:Bacteroides / Q000187:drug effects; D003762:Dental Instruments; D003774:Dental Plaque Index; D012534:Dental Scaling; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000188:drug therapy* / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000187:drug effects; D016037:Single-Blind Method; D013752:Tetracycline / Q000008:administration & dosage*; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline