KITENIN increases invasion and migration of mouse squamous cancer cells and promotes pulmonary metastasis in a mouse squamous tumor model.
KAI1 C-terminal interacting tetraspanin (KITENIN) is reported to promote metastasis in mouse colon cancer models. We investigated the role of KITENIN on the progression of squamous cell carcinoma (SCC). In a preliminary clinical study using resected tissues from head and neck SCC patients, KITENIN was highly expressed in tumors and metastatic lymph nodes, while KAI1 was more increased in adjacent mucosa than in tumor. KITENIN-transfected mouse squamous cancer (SCC VII/KITENIN) cells showed significantly higher invasion, migration, and proliferation than empty vector-transfected cells. In syngeneic mouse squamous tumor models, more increased tumor volume and enhanced lung metastasis were found in SCC VII/KITENIN cells-injected mice. Thus, KITENIN increases invasion and migration of squamous cancer cells and thereby promotes distant metastasis in mouse squamous tumor models.
Lee
Joon Kyoo
JK
Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hak-Dong 5, Dong-Ku, Kwangju 501-190, South Korea.
Bae
Jeong A
JA
Sun
Eun Gene
EG
Kim
Hee Dae
HD
Yoon
Tae Mi
TM
Kim
Kag
K
Lee
Ji Hee
JH
Lim
Sang Chul
SC
Kim
Kyung Keun
KK
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000556:secondary*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D004195:Disease Models, Animal; D005260:Female; D005822:Genetic Vectors; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000556:secondary*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; D008565:Membrane Proteins; D012333:RNA, Messenger; C488287:VANGL1 protein, human