Cryptochrome-1 expression: a new prognostic marker in B-cell chronic lymphocytic leukemia.
Chronic lymphocytic leukemia is an adult-onset leukemia with a heterogeneous clinical behavior. When chronic lymphocytic leukemia cases were divided on the basis of IgV(H) mutational status, widely differing clinical courses were revealed. Since IgV(H) sequencing is difficult to perform in a routine diagnostic laboratory, finding a surrogate for IgV(H) mutational status seems an important priority. In the present study, we proposed the use of Cryptochrome-1 as a new prognostic marker in early-stage chronic lymphocytic leukemia. Seventy patients (Binet stage A, without treatment) were included in the study. We correlated Cryptochrome-1 mRNA with well established prognostic markers such as IgV(H) mutations, ZAP70, LPL or CD38 expression and chromosomal abnormalities. High Cryptochrome-1 expression correlated with IgV(H) unmutated samples. In addition, Cryptochrome-1 was a valuable predictor of disease progression in early-stage chronic lymphocytic leukemia, therefore it can be introduced in clinical practice with the advantage of a simplified method of quantification.
Lewintre
Eloisa Jantus
EJ
Molecular Haematology Laboratory, Prince Felipe Research Centre, Valencia, Spain. ejantus@cipf.es
Martín
Cristina Reinoso
CR
Ballesteros
Carlos García
CG
Montaner
David
D
Rivera
Rosa Farrás
RF
Mayans
José Ramón
JR
García-Conde
Javier
J
D015415:Biomarkers; D056931:Cryptochromes; D005420:Flavoproteins / Q000235:genetics*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*
D015415:Biomarkers; D056931:Cryptochromes; D005420:Flavoproteins; D012333:RNA, Messenger