[Study on fingerprints of Citrus aurantium from different places by capillary electrophoresis].
OBJECTIVE
To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality.


METHODS
The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained.


RESULTS
There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996.


CONCLUSIONS
The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.
Luo
Guang-Ming
GM
Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. jzlgm11@163.com
Tan
Xiao-Hui
XH
Xu
Li-Fang
LF
Yang
Ya-Qin
YQ
Yang
Shi-Lin
SL
D001881:Borates / Q000737:chemistry; D002957:Citrus / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D001881:Borates; D004573:Electrolytes