[Cerebrospinal fluid amyloid beta and tau protein: biomarkers for Alzheimer's disease].
OBJECTIVE
Introduction of acetylcholine esterase inhibitors as a symptomatic treatment of Alzheimer's disease (AD) has additionally highlighted the importance of diagnostic markers in cerebrospinal fluid (CSF) for early AD diagnosis: low level of 42 amino acid form of amyloid-beta peptide (Abeta42), and levels of tau protein (T-tau) and phosphorylated tau protein (P-tau). The aim of this study was to diagnostic potential of CSF biomarkers T-tau, P-tau and Abeta42 as biochemical markers for AD.


METHODS
Lumbar puncture was performed in 63 patients with AD and 26 control subjects who passed orthopedic surgery. The Innotest, ELISA sandwich test (Innogenetics - Belgium) was used for measuring the levels of T-tau, P-tau and Abeta342.


RESULTS
The patients and the control group did not differ in age, education and sex. Mean levels of CSF T-tau and P-tau were significantly higher in the patients with AD (p < 0.001) compared to the control group, in contrast to significantely lower CSF Abeta42 in AD group (p < 0.001). A significant progressive decrease of Abeta42, as well as significant progressive increase of T-tau and P-tau was found among AD subgroups (according to MMSE staging) and controls.


CONCLUSIONS
The obtained results suggest that these biomarkers may be supportive in the diagnosis of AD, especially in the early course of the disease and could be used in the routine clinical practice considering the approaching target therapeutics.
Mandić
Gorana
G
Institut za neurologiju, Klinicki centar Srbije, Beograd. drmandic@drenik.net
Marković
Ivanka
I
Ostojić
Marija
M
Stojković
Tanja
T
Misirlić-Dencić
Sonja
S
Zivanović-Radnić
Tatjana
T
Stefanović
Rodoljub
R
Bumbasirević
Marko
M
Stefanova
Elka
E
Kostić
Vladimir
V
D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008297:Male; D016875:tau Proteins / Q000134:cerebrospinal fluid*
D016229:Amyloid beta-Peptides; D015415:Biomarkers; D016875:tau Proteins