[Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro].
OBJECTIVE
To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.


METHODS
SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.


RESULTS
The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P < 0.05).


CONCLUSIONS
The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.
Guo
Xue-hua
XH
Department of Cell Biology, School of Basic Medical Science, The Second Hospital, Jilin University, Changchun, Jilin 130021, China. gxh_106@163.com
Lin
Yong
Y
Wang
Ye-ling
YL
Zheng
Lian-wen
LW
D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008297:Male; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects*; D019089:Stem Cell Factor / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
D036341:Intercellular Signaling Peptides and Proteins; D053605:Leukemia Inhibitory Factor; D019089:Stem Cell Factor; D016222:Fibroblast Growth Factor 2