title: Quantitative analysis of inflammatory cytokines expression in peripheral blood mononuclear cells of the ferret (Mustela putorius furo) using real-time PCR.
abstract: This study describes the expression pattern of cytokines, interferon-gamma (IFN-gamma), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) and IL-10, produced by LPS stimulation in peripheral blood mononuclear cells (PBMCs) of the ferret (Mustela putorius furo). Real-time PCR was used with TaqMan probes, which were modified by dual-labeled probes (TAMRA/FAM), quantitative analysis of cytokine mRNA comparing the cytokine with the housekeeping gene, ferret GAPDH, as the relative C(t) value. Expression peaks of IFN-gamma, TNF-alpha, IL-6 mRNA occurred 2 h after LPS stimulation, whereas the IL-10 peak was 8 h post-LPS. In the present study, peak cytokine expression was detected within 8 h, similar to several other mammalian studies. This current study provides baseline information on inflammatory cytokines of ferret PBMCs.
authors.affiliation: Nihon University Veterinary Research Center, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakata
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D005289:Ferrets / Q000097:blood / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger