[Cloning, expression and antiviral activity of arctic fox (Alopex lagopus) interferon-gamma gene].
In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma(VuIFN-gamma), We have isolated the cDNA encoding arctic fox (Alopex lagopus) VuIFN-gamma. This cDNA encodes a 23 amino acid signal peptide and a 144 amino acid mature protein, which shares 99.8% or 99.4% for nucleotide identity with silver fox and canine, respectively, and 100% for amino acid identity. Expression of recombinant mature arctic fox interferon gamma (mVuIFN-gamma) in bacterial system was confirmed by SDS-PAGE and Western blotting analysis. Recombinant VuIFN-gamma showed higher antiviral activity against vesicular stomatitis virus in cultured Vero and MDCK by inhibiting virus induced cytopathic effect, In view of the immunomodulatory and antiviral activities of VuIFN-gamma, it may provide a basis for further research on antiviral therapy of recombinant VuIFN-gamma in economic animal practice.
Zhang
Hailing
H
Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China.
Chai
Xiuli
X
Luo
Guoliang
G
Wang
Fengxue
F
Yi
Li
L
Shao
Xiqun
X
Yan
Xijun
X
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005589:Foxes / Q000235:genetics*; D007371:Interferon-gamma / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology*
D000998:Antiviral Agents; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma