title: A novel molecularly imprinted sensor for selectively probing imipramine created on ITO electrodes modified by Au nanoparticles.
abstract: A sensitive molecularly imprinted electrochemical sensor was created for selective detection of a tricyclic antidepressant imipramine by combination of Au nanoparticles (Au-NPs) with a thin molecularly imprinted film. The sensor was fabricated onto the indium tin oxide (ITO) electrode via stepwise modification of Au-NPs by self-assembly and a thin film of molecularly imprinted polymers (MIPs) via sol-gel technology. It was observed that the molecularly imprinted film displayed excellent selectivity towards the target molecule imipramine. Meanwhile, the introduced Au-NPs exhibited noticeable catalytic activities towards imipramine oxidation, which remarkably enhanced the sensitivity of the imprinted film. Due to such combination, the as-prepared sensor responded quickly to imipramine, within only 1 min of incubation. The differential voltammetric anodic peak current was linear to the logarithm of imipramine concentration in the range from 5.0x10(-6) to 1.0x10(-3) mol L(-1), and the detection limits obtained was 1.0x10(-9) mol L(-1). This method proposed was successfully applied to the determination of imipramine in drug tablets, and proven to be reliable compared with conventional UV method. These results reveal that such a sensor fulfills the selectivity, sensitivity, speed and simplicity requirements for imipramine detection, and provides possibilities of clinical application in physiological fluids.
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhou
authors.forename: Huixiang
authors.initials: H
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D018759:Adrenergic Uptake Inhibitors / Q000032:analysis; D000929:Antidepressive Agents, Tricyclic / Q000032:analysis; D004566:Electrodes; D006046:Gold; D007099:Imipramine / Q000032:analysis*; D053768:Metal Nanoparticles; D054802:Molecular Imprinting / Q000379:methods*; D017971:Tin Compounds
chemical_list: D018759:Adrenergic Uptake Inhibitors; D000929:Antidepressive Agents, Tricyclic; D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold; D007099:Imipramine