Use of mouse models for the analysis of human disease.
The first half of this unit discusses the two main approaches to developing genetically engineered mouse models of human disease (i.e., transgenesis and gene targeting). The experimental steps for each method are discussed. Potential pitfalls, advantages, and limitations are also addressed. The second half of the unit describes two mouse models of hypertrophic cardiomyopathy, which illustrate the value of studying such models of human disease, both in terms of understanding disease pathogenesis as well as potential new therapeutic avenues.
Semsarian
Christopher
C
Centenary Institute and University of Sydney, Australia.
D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic