Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 1.
Seven in one blow: The efficient formation of mixed disulfides on the thiol-rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins.
Piontek
Christian
C
Bioorganische Chemie, Gebäude NW1, Universität Bayreuth, 95440 Bayreuth, Germany.
Ring
Petra
P
Harjes
Olaf
O
Heinlein
Christian
C
Mezzato
Stefano
S
Lombana
Nelson
N
Pöhner
Claudia
C
Püttner
Markus
M
Varón Silva
Daniel
D
Martin
Andreas
A
Schmid
Franz Xaver
FX
Unverzagt
Carlo
C
D004722:Endoribonucleases / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D006020:Glycopeptides / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D006023:Glycoproteins / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000737:chemistry; D008026:Ligation; D010084:Oxidation-Reduction; D015202:Protein Engineering; D017510:Protein Folding; D020673:Protein Renaturation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000138:chemical synthesis / Q000737:chemistry
D006020:Glycopeptides; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D004722:Endoribonucleases; C029493:ribonuclease C