title: [Association of phytoplasma with Bermuda grass white-leaf disease].
abstract: OBJECTIVE
  Bermuda grass white leaf is an important disease on Bermuda grass all over the world. The aim of this research is to identify the pathogen which leads to Bermuda grass white leaf occurring on the Chinese mainland.
  
  
  METHODS
  PCR amplification technique, sequence analysis and Southern hybridization were used.
  
  
  RESULTS
  A 1.3 kb fragment was amplified by PCR phytoplasma universal primers and total DNA sample extracted from ill Bermuda grass as the amplified template. Sequence analysis of the amplified fragment indicated it clustered into Candidatus Phytoplasm Cynodontis. Southern hybridization analysis showed differential cingulums.
  
  
  CONCLUSIONS
  The pathogen of Bermuda grass white leaf on the Chinese mainland contains phytoplasma, which provides a scientific basis for further identification, prevention and control of the disease.
authors.affiliation: College of Agronomy, South China Agricultural University, Guangzhou 510642, China. weijunworld@126.com
authors.forename: Weijun
authors.initials: W
authors.lastname: Tan
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.forename: Chengchou
authors.initials: C
authors.lastname: Han
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Tan
authors.forename: Juming
authors.initials: J
authors.lastname: Zhang
mesh_terms: D031744:Cynodon / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D045582:Phytoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S