Grafting of human bone marrow stromal cells into spinal cord injury: a comparison of delivery methods.
METHODS
Three groups of 6 rats received subtotal cervical spinal cord hemisections followed with marrow stromal cell (MSC) transplants by lumbar puncture (LP), intravenous delivery (IV), or direct injection into the injury (control). Animals survived for 4 or 21 days.


OBJECTIVE
Cell therapy is a promising strategy for the treatment of spinal cord injury (SCI). The mode of cell delivery is crucial for the translation to the clinic. Injections directly into the parenchyma may further damage already compromised tissue; therefore, less invasive methods like LP or IV delivery are preferable.


BACKGROUND
Human MSC are multipotent mesenchymal adult stem cells that have a potential for autologous transplantation, obviating the need for immune suppression. Although previous studies have established that MSC can be delivered to the injured spinal cord by both LP and IV, the efficacy of cell delivery has not been directly compared with respect to efficacy of delivery and effects on the host.


METHODS
Purified MSC from a human donor were transplanted into the CSF at the lumbar region (LP), into the femoral vein (IV), or directly into the injury (control). After sacrifice, spinal cord sections were analyzed for MSC graft size, tissue sparing, host immune response, and glial scar formation, using specific antibodies and Nissl-myelin staining.


RESULTS
LP delivery of MSC to the injured spinal cord is superior to IV delivery. Cell engraftment and tissue sparing were significantly better after LP delivery, and host immune response after LP delivery was reduced compared with IV delivery.


CONCLUSIONS
LP is an ideal minimally invasive technique to deliver cellular transplants to the injured spinal cord. It is superior to IV delivery and, together with the potential for autologous transplantation, lends itself for clinical application.
Paul
Courtney
C
Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USA.
Samdani
Amer F
AF
Betz
Randal R
RR
Fischer
Itzhak
I
Neuhuber
Birgit
B
D000328:Adult; D053687:Adult Stem Cells / Q000276:immunology / Q000637:transplantation*; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005268:Femoral Vein; D006801:Humans; D015552:Injections, Intralesional; D007275:Injections, Intravenous; D009457:Neuroglia / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology / Q000601:surgery*; D013119:Spinal Cord Injuries / Q000276:immunology / Q000473:pathology / Q000601:surgery*; D013129:Spinal Puncture; D017154:Stromal Cells / Q000276:immunology / Q000637:transplantation*; D013997:Time Factors