title: Coordinate integrin and c-Met signaling regulate Wnt gene expression during epithelial morphogenesis.
abstract: Integrin receptors for the extracellular matrix and receptor tyrosine kinase growth factor receptors represent two of the major families of receptors that transduce into cells information about the surrounding environment. Wnt proteins are a major family of signaling molecules that regulate morphogenetic events. There is presently little understanding of how the expression of Wnt genes themselves is regulated. In this study, we demonstrate that alpha3beta1 integrin, a major laminin receptor involved in the development of the kidney, and c-Met, the receptor for hepatocyte growth factor, signal coordinately to regulate the expression of Wnt7b in the mouse. Wnt signals in turn appear to regulate epithelial cell survival in the papilla of the developing kidney, allowing for the elongation of epithelial tubules to form a mature papilla. Together, these results demonstrate how signals from integrins and growth factor receptors can be integrated to regulate the expression of an important family of signaling molecules so as to regulate morphogenetic events.
authors.affiliation: Department of Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Liu
authors.forename: Nibedita
authors.initials: N
authors.lastname: Chattopadhyay
authors.forename: Shan
authors.initials: S
authors.lastname: Qin
authors.forename: Charles
authors.initials: C
authors.lastname: Szekeres
authors.forename: Tetyana
authors.initials: T
authors.lastname: Vasylyeva
authors.forename: Zhen X
authors.initials: ZX
authors.lastname: Mahoney
authors.forename: Mary
authors.initials: M
authors.lastname: Taglienti
authors.forename: Carlton M
authors.initials: CM
authors.lastname: Bates
authors.forename: Harold A
authors.initials: HA
authors.lastname: Chapman
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Miner
authors.forename: Jordan A
authors.initials: JA
authors.lastname: Kreidberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D002470:Cell Survival; D017931:DNA Primers / Q000235:genetics; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017228:Hepatocyte Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D039201:Integrin alpha3beta1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D007797:Laminin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009024:Morphogenesis; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D039201:Integrin alpha3beta1; D007797:Laminin; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; C095937:Wnt7b protein, mouse; C097043:laminin alpha5; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met