Recombinant human decorin inhibits TGF-beta1-induced contraction of collagen lattice by hypertrophic scar fibroblasts.
Decorin was reported to bind transforming growth factor-beta (TGF-beta(1)) and neutralise some of its activity as a key regulator of wound contraction and hypertrophic scar formation. In this study, we investigated whether recombinant human decorin affected TGF-beta(1)-induced fibroblast contractile activity, by using fibroblast-populated collagen lattice with decorin added to the collagen gel. Hypertrophic scar fibroblasts showed greater basal contraction of collagen gels than normal fibroblasts, and the addition of TGF-beta(1) significantly enhanced this. Decorin inhibited both the basal and TGF-beta(1)-enhanced contraction of collagen gel by both normal and hypertrophic scar fibroblasts. Decorin also inhibited TGF-beta(1)-induced alpha-smooth muscle actin (alpha-SMA), plasminogen activator inhibitor-1 (PAI-1) protein and mRNA expressions in normal and hypertrophic scar fibroblasts. These results suggest that decorin may have therapeutic potential for excessive skin contraction as observed in hypertrophic scarring.
Zhang
Zhi
Z
Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China. zhangzhicc48@163.com
Garron
Tania M
TM
Li
Xiao-Jian
XJ
Liu
Yan
Y
Zhang
Xiong
X
Li
Ye-Yang
YY
Xu
Wei-Shi
WS
D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D003286:Contracture / Q000517:prevention & control; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D005782:Gels; D006801:Humans; D009130:Muscle, Smooth / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D011509:Proteoglycans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
C541116:ACTA2 protein, human; D000199:Actins; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005782:Gels; D017395:Plasminogen Activator Inhibitor 1; D011509:Proteoglycans; D012333:RNA, Messenger; D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1; D003094:Collagen