Fibronectin promotes the phorbol 12-myristate 13-acetate-induced macrophage differentiation in myeloid leukemia cells.
The extracellular matrix plays a critical role in macrophage maturation. In this study, the HL60 cell line was used as a model of leukemic myeloid cell differentiation. We assessed the ability of HL60 cells cultured on fibronectin substratum prior to phorbol 12-myristate 13-acetate (PMA) induction to differentiate into terminally differentiated macrophages. Beside their distinctive macrophage morphology, they expressed antigen receptors CD14, TLR2, TLR4 and CD68, and displayed enhanced phagocytic activity and production of reactive oxygen species. Expression of CD13, CD33, CD15 and alpha-naphthyl-acetate esterase was also maintained, however, differentiated HL60 cells were HLA-DR and CD1a negative. Here, we describe the enhanced capacity of fibronectin-adherent HL60 cells to differentiate into macrophages in response to PMA.
Esendagli
Gunes
G
Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey. gunes.esendagli@lycos.com.
Canpinar
Hande
H
Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey.
Yilmaz
Guldal
G
Department of Pathology, Faculty of Medicine, Ankara University, Ankara, Turkey.|Department of Pathology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Kaymaz
F Figen
FF
Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Kansu
Emin
E
Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey.
Guc
Dicle
D
Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey.
D005353:Fibronectins / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D008264:Macrophages / Q000166:cytology; D022423:Myeloid Cells / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005353:Fibronectins; D013755:Tetradecanoylphorbol Acetate