title: Proteomic and genomic analysis of PITX2 interacting and regulating networks.
abstract: Pituitary homeobox 2 (PITX2) is a homeodomain transcription factor that has a substantial role in cell proliferation and differentiation in various tissues. In this report, we have conducted a systematic study, using proteomic and genomic approaches, to characterize PITX2-interacting proteins and PITX2-regulating genes. We identified four novel PITX2-associated protein partners Y box binding factor-1, heterogeneous ribonucleoprotein K, nucleolin and heterogeneous nuclear ribonucleoprotein U in mass spectrometry analysis. We also found that overexpression of PITX2 upregulated 868 genes (2-25-fold) and downregulated 191 genes (2-15-fold) in DNA microarray analysis. These data provide an insightful perspective for further studying PITX2 function and mechanism of action.
authors.affiliation: Biomedical Science Graduate Program, Marshall University, Huntington, WV 25755, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.forename: Kan
authors.initials: K
authors.lastname: Huang
authors.forename: Goran
authors.initials: G
authors.lastname: Boskovic
authors.forename: Yulia
authors.initials: Y
authors.lastname: Dementieva
authors.forename: James
authors.initials: J
authors.lastname: Denvir
authors.forename: Donald A
authors.initials: DA
authors.lastname: Primerano
authors.forename: Guo-Zhang
authors.initials: GZ
authors.lastname: Zhu
mesh_terms: D001665:Binding Sites; D002460:Cell Line; D005786:Gene Expression Regulation; D023281:Genomics; D018398:Homeodomain Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology; D011485:Protein Binding; D040901:Proteomics; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D000097577:Homeobox Protein PITX2
chemical_list: D018398:Homeodomain Proteins; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2