title: Molecular survey for Anaplasma bovis and Anaplasma phagocytophilum infection in cattle in a pastureland where sika deer appear in Hokkaido, Japan.
abstract: Anaplasma bovis and Anaplasma phagocytophilum infection were examined by species-specific nested polymerase chain reaction in cattle in a pastureland where sika deer appear in Hokkaido, Japan. Of the 78 cattle examined, 12 (15%) and 1 (1%) tested positive for infection by A. bovis and A. phagocytophilum, respectively. One cattle tested positive for both. A. phagocytophilum infection rates were significantly lower in cattle than in sika deer (46%), but the infection rate by A. bovis was not significantly different between cattle (15%) and sika deer (23%). The strain of A. phagocytophilum detected in this study may possess significantly lower virulence or infectivity in cattle hosts. No clinical symptoms were recorded in the positive cattle, and morulae were not detected in the blood smears.
authors.affiliation: Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
authors.lastname: Jilintai
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Seino
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hayakawa
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hata
authors.forename: Seiji
authors.initials: S
authors.lastname: Kondo
authors.forename: Kotaro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
mesh_terms: D000709:Anaplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000821:virology; D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003625:Data Collection; D003670:Deer / Q000235:genetics / Q000382:microbiology*; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D018884:Ixodes / Q000382:microbiology*; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D014774:Virulence
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S