Lack of specificity of antibodies directed against human beta-adrenergic receptors.
The present study was designed to investigate if antibodies against beta-adrenergic receptors (betaARs) can be used to determine expression of betaAR in human myocardium. Western blotting was performed to investigate the specificity of antibodies directed against beta(1)AR and beta(2)AR in human left ventricular tissue. A comparison was made between cardiac tissue from patients with idiopathic dilated cardiomyopathy and ischemic heart disease and nonfailing donors. The antibodies directed against beta(1)AR and beta(2)AR recognized several protein bands at different molecular weights. Moreover, both antibodies also recognized multiple proteins in Chinese hamster ovary cells expressing beta(1)AR, beta(2)AR, and even beta(3)AR. betaAR antibodies are not specific and are not suited to study expression of betaAR in human myocardium.
Hamdani
Nazha
N
Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands. nazha.hamdani@vumc.nl
van der Velden
Jolanda
J
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006352:Heart Ventricles / Q000737:chemistry; D006801:Humans; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D018342:Receptors, Adrenergic, beta-1 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D018343:Receptors, Adrenergic, beta-2 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D022702:Receptors, Adrenergic, beta-3 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D014162:Transfection
D000906:Antibodies; D011943:Receptors, Adrenergic, beta; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2; D022702:Receptors, Adrenergic, beta-3