[Research on optimization system on tissue culture and rapid propagation of Pueraria mirifica].
OBJECTIVE
To study the tissue culture and rapid-proliferation techniques of Pueraria mirifica.


METHODS
The tender branch were used as explants and cultivated in different media. The optimum media for inducing buds, proliferation and rooting were selected by adjusting the kinds and doses of plant hormones and special compounds.


RESULTS
The medium of MS + IBA 0.05 mg L(-1) + BA 0.5 mg L(-1) was suitable for buds inducing and could be used in the first generation cultivation; MS + IBA 0. 02 mg L(-1) + BA 0.2 mg L(-1) and MS +BA 0.1 mg L(-1) were employed by turns in subculture, 25 days propagation coefficient was 3.0; and the medium of 1/2MS + IBA 0.1 mg L(-1) + IAA 0.2 mg L(-1) + C (special compound) 10 mg L(-1) was used for roots inducing, the rooting rate was 76.9%. Rooting plantlets were transplanted in spring and summer; the surviving rate was 81.0%.


CONCLUSIONS
This technique system could be employed for rapid propagation of P. mirifica.
Huang
Ning-Zhen
NZ
Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Academia Sinica, Guilin 541006, China.
Tang
Feng-Luan
FL
He
Jin-Xiang
JX
Fu
Chuan-Ming
CM
He
Cheng-Xin
CX
Guo
Lun-Fan
LF
Wang
Xin-Gui
XG
D029908:Pueraria / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*