title: Human fetal colon cells and colon cancer cells respond differently to butyrate and PUFAs.
abstract: We verified the hypothesis suggesting modulation of the effects of sodium butyrate (NaBt) by omega-3 or omega-6 PUFAs. Comparing the response of human colon epithelial cell lines of fetal (FHC) and adenocarcinoma (HT-29, HCT-116) origin, we detected significant differences in proliferation, differentiation and apoptotic response to the treatment of NaBt, arachidonic or docosahexaenoic acids and their combination. While in FHC and HT-29 cells NaBt induced G0/G1 arrest, differentiation and low level of apoptosis, in HCT-116 cells G2/M arrest, no differentiation and high degree of apoptosis were detected. Moreover, in FHC cells significant potentiation of apoptosis accompanied by increased arrest in the cell cycle, cell detachment and decrease in differentiation were detected after combined treatment with NaBt and both PUFAs. Changes in cytokinetics induced by fatty acids were accompanied by membrane lipid unpacking, reactive oxygen species (ROS) production, and decrease in mitochondrial membrane potential (MMP). Detection of caspase-3 activation and dynamic modulation of Mcl-1 protein expression imply their possible role in both cell differentiation and apoptotic response. Our results support the concept of modulation of NaBt effects by PUFAs, especially of omega-3 type, in colonic cells in vitro with diverse impact in cell lines derived from normal or neoplastic epithelium.
authors.affiliation: Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
authors.forename: Jirina
authors.initials: J
authors.lastname: Hofmanová
authors.forename: Alena
authors.initials: A
authors.lastname: Vaculová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Koubková
authors.forename: Martina
authors.initials: M
authors.lastname: Hýzd'alová
authors.forename: Alois
authors.initials: A
authors.lastname: Kozubík
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003106:Colon / Q000187:drug effects* / Q000196:embryology; D003110:Colonic Neoplasms / Q000473:pathology*; D004847:Epithelial Cells / Q000187:drug effects; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005333:Fetus; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002087:Butyrates; D005231:Fatty Acids, Unsaturated; D008563:Membrane Lipids