title: Robust dosage PCR (RD-PCR) for highly accurate dosage analysis.
abstract: Clinical diagnostic and epidemiological assays would benefit from accurate detection of duplications and deletions commonly missed by conventional methods of polymerase chain reaction (PCR) amplification and sequencing of individual exons. Robust dosage-PCR (RD-PCR) is a quantitative PCR method that co-amplifies a target template and an endogenous internal control (an autosomal and an X-chromosomal segment) for detection of these mutations. RD-PCR has the advantage of high accuracy and consistency, rapid assay development, widely available controls, and gene dosage over a wide dynamic range.
authors.affiliation: City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA.
authors.forename: Vu Q
authors.initials: VQ
authors.lastname: Nguyen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Steve S
authors.initials: SS
authors.lastname: Sommer
mesh_terms: D017353:Gene Deletion; D018628:Gene Dosage; D020440:Gene Duplication; D050172:Genes, X-Linked; D016133:Polymerase Chain Reaction / Q000379:methods*