[Application of SELDI-TOF-MS technology in study of laryngeal carcinoma biomarkers].
OBJECTIVE
In order to establish a primary diagnosis model of laryngeal carcinoma, we explore the changes of proteomic spectra in serum from patients with laryngeal carcinoma and healthy controls.


METHODS
Proteomic spectra of 32 serum samples from laryngeal carcinoma (18 cases of glottis and 14 of supraglottis) patients and 38 from healthy people whose age and sex was matched were generated by IMAC3-Cu protein chip array and SELDI-TOF-MS (surface was enhanced laser desorption/ionization time-of-flight mass spectrometry). The discriminatory profiling between cancer and normal samples was analyzed by Biomarker Wizard software and Biomarker Pattern software.


RESULTS
Fifteen differentially expressed proteins in serum were screened by analysis of proteomic spectra of preoperative patients and normal subjects. Eight proteins (3795.04, 5068.18, 5339.78, 5908.28, 6629.74, 9266.32, 13879.6 and 14037.1) were obtained for making up patterns that was able to class the patients team and normal team correctly. Corresponding correct ratio were 87.50% (28/32) and 86. 84% (33/38).


CONCLUSIONS
SEI.DI-TOF-MS Protein Chip technology is a new tool for the early diagnosis of laryngeal carcinoma.
Xu
Ying
Y
Department of Otorhinolaryngology-Head and Neck Surgery, the First Hospital of Shanxi Medical University, Taiyuan, 030001, China.
Huangfu
Hui
H
Wang
Binquan
B
Cheng
Yan
Y
Zhang
Yanting
Y
D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor