Unusual sterolic mixture, and 24-isopropylcholesterol, from the sponge Ciocalypta sp. reduce cholesterol uptake and basolateral secretion in Caco-2 cells.
An unusual sterolic mixture (82.3% of 24-isopropylated sterols) and its major component, 24-isopropylcholesterol, isolated from a marine sponge, Ciocalypta sp. (Halichondriidae), reduce cholesterol uptake, basolateral secretion and ACAT-2 mRNA expression and increase the expression of ABCA1 mRNA in Caco-2 cells. The decreases of cholesterol uptake and secretion induced by 24-isopropylcholesterol alone were more than that of both the sterolic mixture and beta-sitosterol. These data add a new sterol, 24-isopropylcholesterol, to sterols that may reduce intestinal cholesterol absorption.
Do
Hong Quang
HQ
Faculté de Pharmacie, Laboratoire de Biochimie pharmaceutique, Mer-Molécules-Santé, EA 2160, Université de Nantes, Nantes, France.
Van Landeghem
Laurianne
L
Wielgosz-Collin
Gaëtane
G
Takoudju
Martin
M
Huvelin
Jean-Michel
JM
Kornprobst
Jean-Michel
JM
Bard
Jean-Marie
JM
Barnathan
Gilles
G
Nazih
Hassan
H
D000818:Animals; D018938:Caco-2 Cells; D002784:Cholesterol / Q000378:metabolism*; D003684:Dehydrocholesterols / Q000494:pharmacology; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects; D011161:Porifera; D012333:RNA, Messenger / Q000032:analysis; D012855:Sitosterols / Q000494:pharmacology; D002785:Sterol O-Acyltransferase / Q000235:genetics; D013261:Sterols / Q000494:pharmacology*; D000097587:Sterol O-Acyltransferase 2
D002784:Cholesterol; D003684:Dehydrocholesterols; D012333:RNA, Messenger; D012855:Sitosterols; D002785:Sterol O-Acyltransferase; D013261:Sterols; D000097587:Sterol O-Acyltransferase 2; C516247:24-isopropenyl-22-dehydrocholesterol; C025473:gamma-sitosterol