title: Mevinolin enhances osteogenic genes (ALP, type I collagen and osteocalcin), CD44, CD47 and CD51 expression during osteogenic differentiation.
abstract: OBJECTIVE
  In this study, we evaluated the effect of mevinolin on the expressions of osteogenic genes and surface molecules expression during osteogenesis.
  
  
  METHODS
  D1 cells were cultured in osteogenic differentiation medium (ODM) for 6 days, treated with mevinolin for 2 days, and then subjected to alizarin red S staining, MTT assays, alkaline phosphatase (ALP) activity determinations, energy dispersive X-ray spectrophotometry (EDX), real-time PCR, Western blot, fluorescence microscopy and FACS analysis.
  
  
  RESULTS
  Mevinolin is commonly prescribed and widely used to lower cholesterol levels, and offers an important, effective approach to the treatment of hypercholesterolemia and arteriosclerosis. However, the direct effect of mevinolin on osteogenesis in vitro has not been clarified. ODM has been previously shown to increase the osteoblast differentiation of D1 cells. In the present study, we investigated the expressions of osteogenic genes and surface molecules during osteoblast differentiation induced by mevinolin. We found that the induction of ALP, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells.
  
  
  CONCLUSIONS
  Our data show that mevinolin enhances the expressions of proteins and surface molecules related to osteogenesis.
authors.affiliation: Department of Orthopaedics, Chonnam Natl. Univ. Hwasun Hospital, 160 Ilsimri, Hwasun-gun, Jeonnam, 519-809, Republic of Korea.
authors.forename: Hyung Keun
authors.initials: HK
authors.lastname: Kim
authors.forename: Sang Gwon
authors.initials: SG
authors.lastname: Cho
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Thi Kim Phuong
authors.initials: TK
authors.lastname: Doan
authors.forename: Qin Sheng
authors.initials: QS
authors.lastname: Hu
authors.forename: Rehan
authors.initials: R
authors.lastname: Ulhaq
authors.forename: Eun Kyoo
authors.initials: EK
authors.lastname: Song
authors.forename: Taek Rim
authors.initials: TR
authors.lastname: Yoon
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D051928:CD47 Antigen / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D039564:Integrin alphaV / Q000235:genetics / Q000378:metabolism*; D008148:Lovastatin / Q000494:pharmacology*; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology
chemical_list: D051928:CD47 Antigen; D024042:Collagen Type I; D018960:Hyaluronan Receptors; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D039564:Integrin alphaV; D015675:Osteocalcin; D008148:Lovastatin; D000469:Alkaline Phosphatase