title: Electrophoretic field gradient focusing with on-column detection by fluorescence quenching.
abstract: Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.
authors.affiliation: School of Chemistry, University of Leeds, Leeds, UK. chmrja@leeds.ac.uk
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Ansell
authors.forename: Pilar González
authors.initials: PG
authors.lastname: Tuñón
authors.forename: Yating
authors.initials: Y
authors.lastname: Wang
authors.forename: Peter
authors.initials: P
authors.lastname: Myers
authors.forename: Cornelius F
authors.initials: CF
authors.lastname: Ivory
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Keen
authors.forename: John B C
authors.initials: JB
authors.lastname: Findlay
mesh_terms: D000818:Animals; D004574:Electromagnetic Fields; D004867:Equipment Design; D005456:Fluorescent Dyes; D007525:Isoelectric Focusing / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins