Piezoelectric-excited millimeter-sized cantilever biosensors.
In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in this chapter. The specificity of the sensor is obtained by immobilizing an antibody specific to the antigen of interest. The piezoelectric cantilever sensors are very sensitive and can easily detect a 60 kDa protein at 100 fg/mL concentration. Experimental procedure for carrying out detection of a target analyte is outlined and a sample set of results is included.
Mutharasan
Raj
R
Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA, USA.
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation* / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers