[Study on preparation technology of aconitine liposomes].
OBJECTIVE
To optimize the prescription and preparation of aconitine liposomes.


METHODS
Aconitine liposomes were prepared by ethanol injection method. The encapsulation efficiency was taken as inspection target and the preparation of liposomes were optimized by orthogonal design. HPLC was used to measure the encapsulation efficiency.


RESULTS
The best prescription was aconitine/ lecithin/cholesterol( 1: 15: 5)with phosphate buffer solution (pH 7. 4) as hydration media. The hydration temperature was 60 degrees C.


CONCLUSIONS
The optimized formulation of aconitine liposomes is reasonable in prescription, practicable in technology, high in encapsulation efficiency and good in stability.
Dong
Wen-Wen
WW
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Zhang
Yan-Fang
YF
Liu
Xiao-Ping
XP
Geng
Dan-Qing
DQ
D000157:Aconitine / Q000737:chemistry*; D031841:Aconitum / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002784:Cholesterol / Q000737:chemistry; D004355:Drug Stability; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D010710:Phosphates / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
D008081:Liposomes; D010710:Phosphates; D010713:Phosphatidylcholines; D000431:Ethanol; D002784:Cholesterol; D000157:Aconitine