Embryonic gene expression profiling using microarray analysis.
Microarray technology enables the interrogation of thousands of genes at one time and therefore a systems level of analysis. Recent advances in the amplification of RNA, genome sequencing and annotation, and the lower cost of developing microarrays or purchasing them commercially, have facilitated the analysis of single preimplantation embryos. The present review discusses the components of embryonic expression profiling and examines current research that has used microarrays to study the effects of in vitro production and nuclear transfer.
Marjani
Sadie L
SL
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA. sadie.marjani@yale.edu
Le Bourhis
Daniel
D
Vignon
Xavier
X
Heyman
Yvan
Y
Everts
Robin E
RE
Rodriguez-Zas
Sandra L
SL
Lewin
Harris A
HA
Renard
Jean-Paul
JP
Yang
Xiangzhong
X
Tian
X Cindy
XC
D005307:Fertilization in Vitro / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*