Longitudinal study of Shiga toxin-producing Escherichia coli shedding in sheep feces: persistence of specific clones in sheep flocks.
To provide information on the persistence and maintenance of colonization with Shiga toxin-producing Escherichia coli (STEC) in sheep, pulsed-field gel electrophoresis analysis of STEC isolates (n = 145) belonging to serogroups O5, O91, and O146 from 39 healthy animals was performed in a 12-month longitudinal study carried out with four sheep flocks. At the flock level as well as the individual-animal level, the same clones were obtained on sampling occasions separated by as much as 11 months.
Sánchez
Sergio
S
Patología Infecciosa y Epidemiología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Avda. de la Universidad s/n, 10071 Cáceres, Spain. sergiosp@unex.es
Martínez
Remigio
R
García
Alfredo
A
Blanco
Jorge
J
Blanco
Jesús E
JE
Blanco
Miguel
M
Dahbi
Ghizlane
G
López
Cecilia
C
Mora
Azucena
A
Rey
Joaquín
J
Alonso
Juan M
JM
D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D005838:Genotype; D008137:Longitudinal Studies; D012703:Serotyping; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
D004269:DNA, Bacterial