title: Embryonic gene expression profiling using microarray analysis.
abstract: Microarray technology enables the interrogation of thousands of genes at one time and therefore a systems level of analysis. Recent advances in the amplification of RNA, genome sequencing and annotation, and the lower cost of developing microarrays or purchasing them commercially, have facilitated the analysis of single preimplantation embryos. The present review discusses the components of embryonic expression profiling and examines current research that has used microarrays to study the effects of in vitro production and nuclear transfer.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA. sadie.marjani@yale.edu
authors.forename: Sadie L
authors.initials: SL
authors.lastname: Marjani
authors.forename: Daniel
authors.initials: D
authors.lastname: Le Bourhis
authors.forename: Xavier
authors.initials: X
authors.lastname: Vignon
authors.forename: Yvan
authors.initials: Y
authors.lastname: Heyman
authors.forename: Robin E
authors.initials: RE
authors.lastname: Everts
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Rodriguez-Zas
authors.forename: Harris A
authors.initials: HA
authors.lastname: Lewin
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Renard
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Yang
authors.forename: X Cindy
authors.initials: XC
authors.lastname: Tian
mesh_terms: D005307:Fertilization in Vitro / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*