title: In vitro selection of hemin-binding catalytic RNA.
abstract: Catalytic RNAs with peroxidase activity were obtained by the in vitro selection of RNA aptamer-binding hemin. One of the RNA aptamers selected showed binding affinity to hemin with a dissociation constant of 0.8muM and exhibited high peroxidase activity by forming a complex with hemin. The catalytic efficiency of the RNA-hemin complex was 10-fold higher than that of hemin alone.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Liu
authors.forename: Takuma
authors.initials: T
authors.lastname: Kagahara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D006427:Hemin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009195:Peroxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D016337:RNA, Catalytic; D006427:Hemin; D009195:Peroxidase