title: Quantitative identification of teratoma tissues formed by human embryonic stem cells with TeratomEye.
abstract: An automated vision system, TeratomEye, was developed for the identification of three representative tissue types: muscle, gut and neural epithelia which are commonly found in teratomas formed from human embryonic stem cells. Muscle tissue, a common structure was identified with an accuracy of 90.3% with high specificity and sensitivity greater than 90%. Gut epithelia were identified with an accuracy of 87.5% with specificity and sensitivity greater than 80%. Neural epithelia which were the most difficult structures to distinguish gave an accuracy of 47.6%. TeratomEye is therefore useful for the automated identification of differentiated tissues in teratoma sections.
authors.affiliation: Stem Cell Group, Bioprocessing Technology Institute, A*STAR, 20 Biopolis Way #06 - 01 Centros, Singapore, 138668, Singapore. steve_oh@bti.a-star.edu.sg
authors.forename: Steve Kah Weng
authors.initials: SK
authors.lastname: Oh
authors.forename: Pauline
authors.initials: P
authors.lastname: Chua
authors.forename: Koh Lin
authors.initials: KL
authors.lastname: Foon
authors.forename: Emily
authors.initials: E
authors.lastname: Ng
authors.forename: Angela
authors.initials: A
authors.lastname: Chin
authors.forename: Andre Boon Hwa
authors.initials: AB
authors.lastname: Choo
authors.forename: Rajagopalan
authors.initials: R
authors.lastname: Srinivasan
mesh_terms: D002454:Cell Differentiation; D003584:Cytological Techniques / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D032342:Muscle Cells / Q000166:cytology*; D046569:Neuroepithelial Cells / Q000166:cytology*