Characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading Sphingomonas sp. strain LH128 able to oxidize benz[a]anthracene.
Sphingomonas sp. strain LH128 was isolated from a polycyclic aromatic hydrocarbon (PAH)-contaminated soil using phenanthrene as the sole source of carbon and energy. A dioxygenase complex, phnA1fA2f, encoding the alpha and beta subunit of a terminal dioxygenase responsible for the initial attack on PAHs, was identified and isolated from this strain. PhnA1f showed 98%, 78%, and 78% identity to the alpha subunit of PAH dioxygenase from Novosphingobium aromaticivorans strain F199, Sphingomonas sp. strain CHY-1, and Sphingobium yanoikuyae strain B1, respectively. When overexpressed in Escherichia coli, PhnA1fA2f was able to oxidize low-molecular-weight PAHs, chlorinated biphenyls, dibenzo-p-dioxin, and the high-molecular-weight PAHs benz[a]anthracene, chrysene, and pyrene. The action of PhnA1fA2f on benz[a]anthracene produced two benz[a]anthracene dihydrodiols.
Schuler
Luc
L
Unité de Génie Biologique, Institut des Sciences de la Vie, Université Catholique de Louvain, Place Croix du Sud, 2/19, 1348 Louvain-la-Neuve, Belgium.
Jouanneau
Yves
Y
Chadhain
Sinéad M Ní
SM
Meyer
Christine
C
Pouli
Maria
M
Zylstra
Gerben J
GJ
Hols
Pascal
P
Agathos
Spiros N
SN
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010616:Phenanthrenes / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D012988:Soil Microbiology; D020578:Sphingomonas / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013379:Substrate Specificity
D001426:Bacterial Proteins; D001551:Benz(a)Anthracenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; C031181:phenanthrene; C030935:benz(a)anthracene; D049308:Dioxygenases