Characterization of anti-D monoclonal antibody reagents based on their reactivity with the weak D phenotype.
BACKGROUND
Anti-D monoclonal antibody (MoAb) reagents have improved D typing in routine tests. However, they exhibit a wide range of reactivity with the weak D phenotype depending on the characteristics of the different MoAbs used. We analyzed the reactivity of immunoglobulin (IgM) anti-D by cluster analysis to characterize MoAb that have similar reactivities with the weak D phenotype.


METHODS
We used 36 consecutive samples with weak D phenotype in routine testing and determined their reactivity with different IgM and IgG anti-D MoAbs. The samples were characterized as belonging to a weak D type or category using commercial molecular biology kits.


RESULTS
The various anti-D MoAbs showed a wide grade of reactivity with the weak D samples. Similarities and dissimilarities in the behavior of the anti-D MoAbs with the weak D phenotype samples were detected with cluster analysis and the multidimensional scaling analysis. These analyses indicated different families of MoAbs characterized as having a high degree of homogeneity in their reactivity with the weak D phenotype. Between these MoAb families, the most effective at reacting with the weak D phenotype were RUM-1 and 175-2.


CONCLUSIONS
The results show that it is possible to classify the anti-D MoAbs on the basis of their reactivity with the weak D phenotype. This provides information about different MoAbs' properties on the basis of their belonging to a given of anti-D family.
Lai
Marco
M
Immunohematology Laboratory, Transfusion Centre, Catholic University of Sacred Heart, Rome, Italy. marco_lai@fastwebnet.it
Grasso
Cinzia
C
Boschi
Ilaria
I
D'Onofrio
Giuseppe
G
Pascali
Vincenzo
V
Leone
Giuseppe
G
D000911:Antibodies, Monoclonal / Q000145:classification* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D016000:Cluster Analysis; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000145:classification* / Q000276:immunology; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D018029:Rho(D) Immune Globulin
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin