Administration of bone marrow stromal cells ameliorates experimental autoimmune myasthenia gravis by altering the balance of Th1/Th2/Th17/Treg cell subsets through the secretion of TGF-beta.
Bone marrow stromal cells (BMSCs) are strong candidates for cell therapy against human autoimmune diseases. Intravenous administration of syngenic BMSCs to EAMG-model rats effectively ameliorated the disease, partially through a TGF-beta-dependent mechanism. The proliferative ability of T or B cells from EAMG rats was inhibited by BMSCs at proper cocultured ratios. And the imbalance of Th1, Th2, Th17 and Treg cell subsets accompanied with the development of EAMG was corrected by the administration of BMSCs. These results provide further insights into the pathogenesis of MG, EAMG, and other immune-mediated diseases, and support a potential role for BMSCs in their treatment.
Kong
Qing-Fei
QF
Department of Neurobiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Neurobiology, 157 Bao Jian Road, Harbin 150081, China.
Sun
Bo
B
Bai
Sha-Sha
SS
Zhai
Dong-Xu
DX
Wang
Guang-You
GY
Liu
Yu-Mei
YM
Zhang
Shu-Juan
SJ
Li
Rui
R
Zhao
Wei
W
Sun
Yan-Yan
YY
Li
Na
N
Wang
Qi
Q
Peng
Hai-Sheng
HS
Jin
Lian-Hong
LH
Li
Hu-Lun
HL
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001835:Body Weight; D016026:Bone Marrow Transplantation / Q000379:methods*; D049109:Cell Proliferation; D018920:Coculture Techniques / Q000379:methods; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007136:Immunoglobulins / Q000378:metabolism; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000276:immunology / Q000601:surgery*; D010455:Peptides / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D011950:Receptors, Cholinergic / Q000276:immunology; D017154:Stromal Cells / Q000276:immunology / Q000637:transplantation*; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism*
D016207:Cytokines; D007136:Immunoglobulins; D010455:Peptides; D011950:Receptors, Cholinergic; D016212:Transforming Growth Factor beta