Expression of CPEB, GAPDH and U6snRNA in cervical and ovarian tissue during cancer development.
Persistent infection with high-risk human papillomavirus (HPV) and expression of the proteins E6 and E7 is a prerequisite for development of cervical cancer. The distal non-coding part of E6/E7 messengers from several HPV types is able to downregulate synthesis of a reporter gene through mechanisms with involvement of cytoplasmic polyadenylation elements (CPEs) in the messengers. We here show that the mRNA levels of one of the four known CPE-binding proteins (CPEBs), the CPEB3, were downregulated in HPV-positive cervical cancers, whereas in ovarian cancer the CPEB1 mRNA level was downregulated. In addition, we showed that the RNA levels of the widely used reference marker GAPDH were upregulated in both cancer forms, and the level of the reference marker U6snRNA was upregulated in cervical cancers. Moreover, a possible correlation between the degree of U6snRNA upregulation and cervical cancer propagation was shown. These changes observed in CPEB1 and CPEB3 might indicate regulatory functions of CPEBs in cancer development of HPV-positive and HPV-negative tumors, respectively, and the U6snRNA, GAPDH mRNA and CPEB1 mRNA levels may be useful as tumor markers for genital cancers although further investigations are needed.
Hansen
Christina Neigaard
CN
Institute of Cellular and Molecular Medicine, DNA Tumor Virus Laboratory, University of Copenhagen, Copenhagen, Denmark.
Ketabi
Zohreh
Z
Rosenstierne
Maiken Worsøe
MW
Palle
Connie
C
Boesen
Hans Christian
HC
Norrild
Bodil
B
D052685:Alphapapillomavirus; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D030361:Papillomavirus Infections / Q000150:complications; D012333:RNA, Messenger / Q000032:analysis; D012342:RNA, Small Nuclear / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics*
C490386:CPEB1 protein, human; C513866:CPEB3 protein, human; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C405486:U6 small nuclear RNA; D039221:mRNA Cleavage and Polyadenylation Factors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases