title: [Evaluation of CD4+ CD25+ regulatory T cells in the peripheral blood of recurrent spontaneous abortion patients].
abstract: OBJECTIVE
  To explore the role of CD4+ CD25+ regulatory T cells (CD4+ CD25+ Tr) in the pathogenesis of recurrent spontaneous abortion.
  
  
  METHODS
  Peripheral blood samples were collected from 29 women with unexplainable recurrent spontaneous abortion (the URSA group) and another 20 with normal pregnancy (the control group). The percentage of CD4+ CD25+ Tr in the peripheral blood was measured by flow cytometry.
  
  
  RESULTS
  The rate of CD4+ CD25bright Tr in the URSA patients ([1.98 +/- 0.96]%) was significantly lower than that in the control group ([3.21 +/- 1.25]%, P < 0.05), while the percentages of CD4+ CD25+ and CD4+ CD25dim and the ratio of CD4+ CD25bright/CD4+ were not significantly different between the two groups (P > 0.05).
  
  
  CONCLUSIONS
  URSA might be associated with the decreased percentage of CD4+ CD25bright Tr, which plays an important role in fetomaternal immunologic tolerance.
authors.affiliation: PLA Research Institute of Clinical Laboratory Medicine, School of Clinical Medicine Nanjing University Medical College, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. xiaxy@androl.cn
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Xia
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Ting
authors.initials: T
authors.lastname: Xiong
authors.forename: Hong-Yong
authors.initials: HY
authors.lastname: Lu
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Ge
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Cui
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000276:immunology*; D000022:Abortion, Spontaneous / Q000097:blood / Q000276:immunology*; D000328:Adult; D015704:CD4 Antigens / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood; D011247:Pregnancy; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit