title: [Informatics analysis of malate dehydrogenase from Taenia saginata asiatica].
abstract: Tools from bioinformatics websites such as NCBI, ExPaSy were used for the analysis. The malate dehydrogenase full-length gene from Taenia saginata asiatica was 1 212 bp in length, with a coding region of 30-1 028 bp and coding 332 amino acids. It was a complete and full-length gene compared with the homologues in GenBank. The protein showed no transmembrane region, with stable physical-chemical characteristics. Three major linear epitopes located aa95-aa100, aa322-aa327 and aa117-aa122, with certain distance from each other on the surface of spatial structure of malate dehydrogenase (MDH). The last one was the linear epitope of Taenia. This cytosolic malate dehydrogenase gene is a potential antigen for diagnosis.
authors.affiliation: Department of Parasitology, Guiyang Medical University, Guiyang 550004, China. mmm_hj@gmc.edu.cn
authors.forename: Jiang
authors.initials: J
authors.lastname: Huang
authors.forename: Xu-Chu
authors.initials: XC
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Yu
authors.forename: Huai-En
authors.initials: HE
authors.lastname: Bao
authors.forename: Shu-Yuan
authors.initials: SY
authors.lastname: Lang
authors.forename: Xing-Jiang
authors.initials: XJ
authors.lastname: Liao
mesh_terms: D000818:Animals; D019295:Computational Biology; D018076:DNA, Complementary; D000939:Epitopes / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics*; D008291:Malate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D041202:Taenia saginata / Q000201:enzymology / Q000235:genetics* / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D000939:Epitopes; D015801:Helminth Proteins; D008291:Malate Dehydrogenase