It takes two to flirt with a dimeric RNase.
In my long RNase-life-time I have had the fortune to entertain with Lelio Mazzarella what I have called special interactions, which not only advanced my knowledge of proteins but also gave pleasure, as one obtains by reading a beautiful book, an inspiring poem, or watching a very good movie. In this article, I recall the more than 30-year long story of these interactions in which "it took two," a structural biologist with his coworkers and a biochemist with his coworkers, to flirt with an unusual, dimeric RNase.
D'Alessio
Giuseppe
G
Department of Structural and Functional Biology, University of Naples Federico II, Via Cintia, Napoli 80126, Italy. dalessio@unina.it
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D018360:Crystallography, X-Ray; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D007527:Isoenzymes; C013241:ribonuclease dimer; D004722:Endoribonucleases; D012260:Ribonucleases; C032523:ribonuclease SPL; D012259:Ribonuclease, Pancreatic