title: Tamoxifen-dependent, inducible Hoxb6CreERT recombinase function in lateral plate and limb mesoderm, CNS isthmic organizer, posterior trunk neural crest, hindgut, and tailbud.
abstract: The ability to generate conditional mutant alleles in mice using Cre-lox technology has facilitated analysis of genes playing critical roles in multiple developmental processes at different times. We used a transgenic Hoxb6 promoter to drive tamoxifen-dependent Cre recombinase expression in several developing systems that serve as major models for elucidating inductive interactions and mechanisms of morphogenesis, including lateral plate mesoderm and descendant limb buds, neural crest progenitors of the neural tube, tailbud, and CNS isthmic organizer. The Hoxb6CreER(T) line gives very rapid and complete recombination over a short time window after a single tamoxifen dose, allowing precise time requirements for gene function to be assessed accurately. Embryonic cells cultured from the Hoxb6CreER(T) line also display rapid recombination ex vivo after tamoxifen exposure. Hence, the Hoxb6CreER(T) line provides a valuable tool for analyzing gene function, as well as lineage tracing studies using genetic cell marking, in several developing systems.
authors.affiliation: Laboratory of Pathology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, USA.
authors.forename: Minh-Thanh
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Jianjian
authors.initials: J
authors.lastname: Zhu
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Nakamura
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Bao
authors.forename: Susan
authors.initials: S
authors.lastname: Mackem
mesh_terms: D000818:Animals; D019521:Body Patterning; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000378:metabolism; D005121:Extremities / Q000196:embryology* / Q000502:physiology; D041981:Gastrointestinal Tract / Q000196:embryology* / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D019426:Integrases / Q000235:genetics*; D008648:Mesoderm / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009432:Neural Crest / Q000196:embryology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013623:Tail / Q000196:embryology* / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D018398:Homeodomain Proteins; C490537:Hoxb6 protein, mouse; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases