Different immunomodulatory effects associated with sub-micrometer particles in ambient air from rural, urban and industrial areas.
Immunomodulatory effects of chemicals adsorbed to particles with aerodynamic diameter below 0.49 microm (PM(0.5)) collected in winter 2001 at three sampling points (industrial area [LPIn], traffic-influenced urban area [LPCi], and control area [LPCo]) of La Plata, Argentina, were investigated. The sampling of particulate matter was carried out with high-volume collectors using cascade impactors. PM(0.5)-adsorbed compounds were hexane-extracted by accelerated solvent extraction. For immunological investigations, human peripheral blood lymphocytes were activated by phytohemagglutinin and exposed to dimethyl-sulfoxide dilutions of PM(0.5)-extracts for 24h. Vitality/proliferation was quantified using MTT, released interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) by ELISA. Cytokine production but not vitality/proliferation was significantly suppressed by all of the highest extract concentrations. Generally, suppression of IFN-gamma by PM(0.5)-extracts was stronger than those of IL-4. Based on administered mass of PM(0.5), all extracts suppressed IFN-gamma production nearly uniform. Contrary, LPCi-extracts exerted maximum IFN-gamma suppression based either on air volume or regarding PM(0.5)-adsorbed PAH. Also the ranking of PM(0.5)-associated effects on IL-4 production differs in dependence of the chosen reference points, either mass or [microg/ml] or air volume [m(3)/ml] related dust quantities in cell culture. Based on the corresponding air volume, LPCi-extracts inhibited IL-4 production to the maximum extend, whereas suppression of IL-4 was comparable based on concentrations. This indicates that not only the disparate PM(0.5)-masses in air cause varying impacts, but also that disparities in PM(0.5)-adsorbed chemicals provoke different effects on immune responses and shifts in the regulatory balance that might have implications for allergy and cancer development.
Wichmann
Gunnar
G
Department of Otolaryngology, Head and Neck Surgery, University of Leipzig, Liebigstrasse 26, D-04103 Leipzig, Germany. Gunnar.Wichmann@medizin.uni-leipzig.de
Franck
Ulrich
U
Herbarth
Olf
O
Rehwagen
Martina
M
Dietz
Andreas
A
Massolo
Laura
L
Ronco
Alicia
A
Müller
Andrea
A
D000393:Air Pollutants / Q000633:toxicity*; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D001118:Argentina; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004391:Dust / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007155:Immunologic Factors; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D009000:Monocytes / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity*; D010316:Particle Size; D010835:Phytohemagglutinins / Q000494:pharmacology; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D012423:Rural Health; D013050:Spectrometry, Fluorescence; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles; D014504:Urban Health
D000393:Air Pollutants; D000395:Air Pollutants, Occupational; D016207:Cytokines; D004391:Dust; D007155:Immunologic Factors; D010835:Phytohemagglutinins; D011084:Polycyclic Aromatic Hydrocarbons; D013778:Tetrazolium Salts; D013844:Thiazoles; D015847:Interleukin-4; D007371:Interferon-gamma; C022616:thiazolyl blue