[The anti-tumour effect of Wuxing soup and its mechanism in inducing apoptosis of tumour cells mediated by calcium].
OBJECTIVE
To confirm the anti-cancer effect and mechanism of Wuxing soup.


METHODS
Inhibition of cellular growth under Wuxing soup treatment was observed by MTT; Apoptosis was detected by gel electrophoresis, transmission electron microscopy and FACS; The concentration of calcium was measured by fluorescence probe.


RESULTS
After SGC-7901 cell being treated by Wuxing soup, it showed that: 1) Wuxing soup could specifically inhibit cancer cells proliferation in a time and dose dependent manner; 2) Typical apoptotic morphological changes and DNA ladder of SGC-7901 cells were observed; 3) calcium inhibitor Bapta AM could reduce the apoptotic rate and protect SGC-7901 cells in a dose dependent manner.


CONCLUSIONS
Wuxing soup has an effective inhibition on cancer cells, and can induce SGC-7901 cells to apoptosis by calcium.
Mo
Fei
F
Graduate School of Fudan University, Shanghai 200032, China.
Hu
Jing-Ying
JY
Gan
Yu
Y
Zhao
Yang-Xing
YX
Zhao
Xin-Tai
XT
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D031170:Arctium / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018552:Daucus carota / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D010945:Plants, Edible / Q000737:chemistry*; D031224:Raphanus / Q000737:chemistry; D013274:Stomach Neoplasms / Q000473:pathology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D002118:Calcium