DNA methylation signature analysis: how easy is it to perform?
Epigenetic changes, or heritable alterations in gene function that do not affect DNA sequence, are rapidly gaining acceptance as co-conspirators in carcinogenesis. Although DNA methylation signature analysis by methylation-specific polymerase chain reaction has been a breakthrough method in speed and sensitivity for gene methylation studies, several factors still limit its application as a routine diagnostic and prognostic test.
Piperi
Christina
C
Department of Biological Chemistry, Medical School, University of Athens, Athens, Greece.
Farmaki
Elena
E
Vlastos
Fotis
F
Papavassiliou
Athanasios G
AG
Martinet
Nadine
N
D052160:Benzothiazoles; D001709:Biotechnology; D018899:CpG Islands; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D019175:DNA Methylation; D003959:Diamines; D044127:Epigenesis, Genetic; D005456:Fluorescent Dyes; D006358:Hot Temperature; D006801:Humans; D009691:Nucleic Acid Denaturation; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods; D011804:Quinolines; D013447:Sulfites
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; D013447:Sulfites; C098022:SYBR Green I; D004247:DNA; C009279:sodium bisulfite