[Effect of nobiletin on tubulin polymerization in vitro].
OBJECTIVE
To investigate the possible mechanisms of nobiletin for anticancer by studying the inhibition effects of nobiletin on tubulin polymerization.


METHODS
In vitro nobiletin was added into the tubulin polymerization-depolymerization system and the absorption values were recorded at 350 nm under 37 degrees C.


RESULTS
As compared with controls, the absorption values in reaction system decreased significantly in nobiletin treatment groups. When nobiletin final concentrations in reaction system were 5.0, 7.5, 10.0 and 12.5 micromol x L(-1), the maximum absorption values were 0 130, 0.109, 0.086 and 0.071 with 16.7%, 30.1%, 44.9% and 54.5% of inhibition rate, respectively. The results suggested that nobiletin could inhibit tubulin polymerization.


CONCLUSIONS
The inhibition effect of nobiletin on tubulin polymerization is the possible mechanism for anticancer.
Liu
Yi
Y
Guangdong Medical College, Guangdong Key Laboratory for Research and Development of Nature Drugs, Zhanjiang 524023, China. plliu78@sina.com
Wu
Ke-Feng
KF
Li
Yan-Ping
YP
D000818:Animals; D047309:Flavones / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D013552:Swine; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism*; D050257:Tubulin Modulators / Q000494:pharmacology*
D047309:Flavones; D014404:Tubulin; D050257:Tubulin Modulators; C008661:nobiletin