Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy.
Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers. Furthermore, collagen orientation in post-UVB-exposed skin with few wrinkles changed from that of UVB-exposed wrinkled skin to that of no-UVB-exposed skin. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging.
Yasui
Takeshi
T
Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan. t-yasui@me.es.osaka-u.ac.jp
Takahashi
Yu
Y
Fukushima
Shuichiro
S
Ogura
Yuki
Y
Yamashita
Toyonobu
T
Kuwahara
Tomohiro
T
Hirao
Tetsuji
T
Araki
Tsutomu
T
D000818:Animals; D020405:Dermis / Q000502:physiology* / Q000528:radiation effects; D024022:Fibrillar Collagens / Q000378:metabolism*; D051379:Mice; D008859:Microscopy, Polarization / Q000379:methods*; D015595:Skin Aging / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D024022:Fibrillar Collagens