title: Phosphatidylinositol-4,5-bisphosphate-dependent facilitation of the ATP-dependent secretory activity in mouse pituitary cells.
abstract: Phosphatidylinositol-4, 5-bisphosphate [PI(4,5)P(2)] has been implicated in the priming of large dense-core vesicles in many secretory cells; however, its role in the Ca(2+)-dependent secretory activity in pituitary cells remains elusive. We assessed the effect of elevated intracellular PI(4,5)P(2) on the kinetics of Ca(2+)-dependent exocytosis, using a whole-cell patch-clamp technique in wild-type mouse melanotrophs from fresh pituitary tissue slices. We found that 1 micromol/L PI(4,5)P(2) significantly increased Ca(2+)-dependent exocytosis of vesicles that need to go through ATP-dependent reactions; however, the exocytosis of release-ready vesicles (ATP-independent release) and voltage-activated Ca(2+) currents remained unaffected. We suggest that PI(4,5)P(2) increases the size of the readily releasable vesicle pool by regulating the effectiveness of vesicular mobilization and fusion in an ATP-dependent manner.
authors.affiliation: European Neuroscience Institute-Göttingen, Göttingen, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Sedej
authors.forename: Iman Singh
authors.initials: IS
authors.lastname: Gurung
authors.forename: Thomas
authors.initials: T
authors.lastname: Binz
authors.forename: Marjan
authors.initials: M
authors.lastname: Rupnik
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004594:Electrophysiology; D005260:Female; D008297:Male; D051379:Mice; D018408:Patch-Clamp Techniques; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium