Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.
The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue.
Vasileiou
Chrysoula
C
Department of Chemistry, Michigan State University, East Lansing, 48824, USA.
Lee
Kin Sing Stephen
KS
Crist
Rachael M
RM
Vaezeslami
Soheila
S
Goins
Sarah M
SM
Geiger
James H
JH
Borhan
Babak
B
D001120:Arginine / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D018698:Glutamic Acid / Q000235:genetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D018168:Receptors, Retinoic Acid / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism
D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D018698:Glutamic Acid; D014212:Tretinoin; D001120:Arginine