Protein kinase C as an effector of lipid-derived second messengers.
Members of the protein kinase C family are major effectors of lipid second messengers. We describe three protocols to assess protein kinase C activity in polymorphonuclear leukocytes (neutrophils). These methods are useful to study the activation and function of protein kinase C in these immune cells. Since neutrophils provide a ready source of human primary tissue, these methods are also useful for pharmacological studies on the protein kinase C system and for evaluation of protein kinase C activators and inhibitors in the context of human primary cells. Furthermore, since protein kinase C activity is determined by a number of lipid-generating signaling systems, the methods described here can also be employed to study the pharmacology of these "upstream" signaling systems.
Paclet
Marie-Hélène
MH
GREPI, TIMC-IMAG, CNRS UMR 5525, Laboratoire d'Enzymologie, CHU de Grenoble, France.
Davidson-Moncada
Jan K
JK
López-Lluch
Guillermo
G
Shao
Dongmin
D
Dekker
Lodewijk V
LV
D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D050356:Lipid Metabolism; D021962:Membrane Microdomains / Q000378:metabolism; D019255:NADPH Oxidases; D009504:Neutrophils / Q000166:cytology / Q000201:enzymology; D011493:Protein Kinase C / Q000032:analysis* / Q000378:metabolism*; D015290:Second Messenger Systems
D007527:Isoenzymes; D019255:NADPH Oxidases; D011493:Protein Kinase C