Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
Schizophrenia is a complex disease, likely to be caused by a combination of serial alterations in a number of genes and environmental factors. The dorsolateral prefrontal cortex (Brodmann's Area 46) is involved in schizophrenia and executes high-level functions such as working memory, differentiation of conflicting thoughts, determination of right and wrong concepts and attitudes, correct social behavior and personality expression. Global proteomic analysis of post-mortem dorsolateral prefrontal cortex samples from schizophrenia patients and non-schizophrenic individuals was performed using stable isotope labeling and shotgun proteomics. The analysis resulted in the identification of 1,261 proteins, 84 of which showed statistically significant differential expression, reinforcing previous data supporting the involvement of the immune system, calcium homeostasis, cytoskeleton assembly, and energy metabolism in schizophrenia. In addition a number of new potential markers were found that may contribute to the understanding of the pathogenesis of this complex disease.
Martins-de-Souza
Daniel
D
Laboratório de Neurociências, Instituto de Psiquiatria, Universidade de São Paulo, Rua. Dr. Ovidio Pires de Campos, no 785, Consolação, São Paulo, SP 05403-010, Brazil.
Gattaz
Wagner F
WF
Schmitt
Andrea
A
Rewerts
Christiane
C
Maccarrone
Giuseppina
G
Dias-Neto
Emmanuel
E
Turck
Christoph W
CW
D000368:Aged; D000369:Aged, 80 and over; D002118:Calcium / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006706:Homeostasis; D006801:Humans; D007107:Immune System / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011180:Postmortem Changes; D017397:Prefrontal Cortex / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012559:Schizophrenia / Q000378:metabolism*; D015398:Signal Transduction
D009419:Nerve Tissue Proteins; D010455:Peptides; D020543:Proteome; D002118:Calcium