In vitro anti-inflammatory activity of carvacrol: Inhibitory effect on COX-2 catalyzed prostaglandin E(2) biosynthesis.
Possible anti-inflammatory effect of carvacrol was evaluated by in vitro cyclooxygenase-2 (COX-2) assay. Carvacrol inhibited production of prostaglandin E(2) catalysed by COX-2 with an IC(50) value of 0.8 microM what is practically the same concentration as the IC(50) obtained for the standard inhibitors indomethacin and NS-398 with values of 0.7 microM and 0.8 microM, respectively. The COX-1 was inhibited approximately at the same rate (IC(50) of 0.7 microM for carvacrol), which suggests non-selective inhibition of both enzyme isoforms. The results of the study demonstrate possible anti-inflammatory potential of this compound due to the inhibition of inducible COX-2 isoform.
Landa
Premysl
P
Joint Laboratory of Institute of Experimental Botany AS CR, Prague 6 - Lysolaje, Czech Republic.
Kokoska
Ladislav
L
Pribylova
Marie
M
Vanek
Tomas
T
Marsik
Petr
P
D000818:Animals; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D000081007:Cymenes; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin / Q000494:pharmacology; D039821:Monoterpenes / Q000494:pharmacology*; D009578:Nitrobenzenes / Q000494:pharmacology; D012756:Sheep; D013449:Sulfonamides / Q000494:pharmacology
D052246:Cyclooxygenase 2 Inhibitors; D000081007:Cymenes; D039821:Monoterpenes; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; C073316:carvacrol; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D007213:Indomethacin