[The development and application of an immunoenzyme assay kit for the detection of compounds of the opiate family in human biological liquids].
Monoclonal antimorphine antibodies both free and conjugated with horse- radish peroxidase have been raised and used to develop an assay kit for the detection of narcotic opiate-based drugs by an immuno-enzyme assay (IEA). The kit contains all ingredients necessary for the enzymatic reaction. A total of 215 urine and blood samples were analysed using the new kit. The results were compared with the data obtained by thin layer chromatography and high-performance liquid chromatography. False negative results were absent while false positive (inconclusive) results were recorded in three cases, probably due to the fact that sensitivity of IEA is higher than that of control methods. It is concluded that the kit may be used in laboratory screening studies for detecting opiates in biological fluids.
Nikolaeva
T L
TL
Belkina
E V
EV
Bogatyreva
E A
EA
Gribkova
S E
SE
Proskurina
N V
NV
Smirnova
V K
VK
Lapenkov
M I
MI
D000701:Analgesics, Opioid / Q000097:blood* / Q000652:urine*; D000818:Animals; D000911:Antibodies, Monoclonal; D002138:Calibration; D005260:Female; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000276:immunology; D009293:Opioid-Related Disorders / Q000097:blood* / Q000652:urine*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods*
D000701:Analgesics, Opioid; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D009020:Morphine; D006735:Horseradish Peroxidase