title: Maturation of the translation inhibitor microcin C.
abstract: Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibition of the McC target, aspartyl-tRNA synthetase.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Metlitskaya
authors.forename: Teymur
authors.initials: T
authors.lastname: Kazakov
authors.forename: Gaston H
authors.initials: GH
authors.lastname: Vondenhoff
authors.forename: Maria
authors.initials: M
authors.lastname: Novikova
authors.forename: Alexander
authors.initials: A
authors.lastname: Shashkov
authors.forename: Timofei
authors.initials: T
authors.lastname: Zatsepin
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Semenova
authors.forename: Natalia
authors.initials: N
authors.lastname: Zaitseva
authors.forename: Vasily
authors.initials: V
authors.lastname: Ramensky
authors.forename: Arthur
authors.initials: A
authors.lastname: Van Aerschot
authors.forename: Konstantin
authors.initials: K
authors.lastname: Severinov
mesh_terms: D001226:Aspartate-tRNA Ligase / Q000037:antagonists & inhibitors / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D053898:Biosynthetic Pathways; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008958:Models, Molecular; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001430:Bacteriocins; D029968:Escherichia coli Proteins; D011500:Protein Synthesis Inhibitors; C020411:microcin; D001226:Aspartate-tRNA Ligase