title: Embryo quality and implantation rate in two different culture media: ISM1 versus Universal IVF Medium.
abstract: OBJECTIVE
  To compare the outcome of two different culture media marketed by the MediCult AS Company (Jyllinge, Denmark)-Universal IVF Medium and ISM1 Medium culture-which, in addition to glucose, pyruvate, and energy-providing components, also contain amino acids, nucleotides, vitamins, and cholesterol.
  
  
  METHODS
  Laboratory and retrospective clinical study.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  A total of 726 patients, undergoing IVF-intracytoplasmic sperm injection procedure, comparable in mean age range, oocyte retrieval, and infertility indication, were included in the study. Laboratory quality and standard procedures were maintained unaffected.
  
  
  METHODS
  Oocyte retrieval, different embryo culture media.
  
  
  METHODS
  Embryo quality, ongoing pregnancy, and implantation rate.
  
  
  RESULTS
  The frequency of good-quality embryos (79% vs. 74%) and the percentages of ongoing pregnancy (27.5% vs. 18%) and implantation rate (15% vs. 10%) were significantly higher in the group treated with ISM1 Medium rather than Universal IVF Medium.
  
  
  CONCLUSIONS
  ISM1 Medium culture seems to improve the performance of embryonic growth and development, as well as increasing the percentage of pregnancy.
authors.affiliation: Mother-Infant Department, Institute of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Modena, Italy. susannaxella@yahoo.it
authors.forename: Susanna
authors.initials: S
authors.lastname: Xella
authors.forename: Tiziana
authors.initials: T
authors.lastname: Marsella
authors.forename: Daniela
authors.initials: D
authors.lastname: Tagliasacchi
authors.forename: Simone
authors.initials: S
authors.lastname: Giulini
authors.forename: Antonio
authors.initials: A
authors.lastname: La Marca
authors.forename: Alessandra
authors.initials: A
authors.lastname: Tirelli
authors.forename: Annibale
authors.initials: A
authors.lastname: Volpe
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002469:Cell Separation; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D018873:Pregnancy Rate; D011786:Quality Control; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology; D055815:Young Adult
chemical_list: D003470:Culture Media