Daptomycin versus Friulimicin B: in-depth profiling of Bacillus subtilis cell envelope stress responses.
The related lipo(depsi)peptide antibiotics daptomycin and friulimicin B show great potential in the treatment of multiply resistant gram-positive pathogens. Applying genome-wide in-depth expression profiling, we compared the respective stress responses of Bacillus subtilis. Both antibiotics target envelope integrity, based on the strong induction of extracytoplasmic function sigma factor-dependent gene expression. The cell envelope stress-sensing two-component system LiaRS is exclusively and strongly induced by daptomycin, indicative of different mechanisms of action in the two compounds.
Wecke
Tina
T
KIT Research Group 11-1, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Zühlke
Daniela
D
Mäder
Ulrike
U
Jordan
Sina
S
Voigt
Birgit
B
Pelzer
Stefan
S
Labischinski
Harald
H
Homuth
Georg
G
Hecker
Michael
M
Mascher
Thorsten
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D017576:Daptomycin / Q000494:pharmacology*; D020869:Gene Expression Profiling; D010455:Peptides / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D010455:Peptides; C416818:friulimicin B; D017576:Daptomycin