title: Disruption of the NADPH-dependent glutamate dehydrogenase affects the morphology of two industrial strains of Penicillium chrysogenum.
abstract: New morphological aspects of Penicillium chrysogenum were found during physiological characterisation of two NADPH-dependent glutamate dehydrogenase mutant strains. A morphological characterisation of the previously constructed strains, together with the two beta-lactam producing industrial recipient strains, was conducted. The reference strains showed a compact structure with highly branched hyphal elements whereas the morphology of the DeltagdhA strains consisting of long elongated hyphal elements with few branches. On solid medium, the hyphal growth unit (length) increased from an average of 47 microm tip(-1) in the reference strains to 117 microm tip(-1) in the DeltagdhA strains and in submerged cultures a decrease of 18% in branching frequency was measured due to the gdhA deletion. P. chrysogenum Wis 54-1255, the ancestor of most production strains was also characterised and this strain showed morphology similar to the industrial strains. Interestingly, the constructed strains showed morphology similar to wild type Aspergillus nidulans another species carrying the penicillin biosynthetic cluster. Thus, the results showed that elimination of glutamate dehydrogenase activity in high producing strains of P. chrysogenum has a radical impact on morphology.
authors.affiliation: Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Building 223, DK-2800 Kgs., Lyngby, Denmark.
authors.forename: Jette
authors.initials: J
authors.lastname: Thykaer
authors.forename: Kanchana
authors.initials: K
authors.lastname: Rueksomtawin
authors.forename: Henk
authors.initials: H
authors.lastname: Noorman
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D001233:Aspergillus nidulans / Q000166:cytology / Q000254:growth & development; D018086:Glutamate Dehydrogenase (NADP+) / Q000235:genetics*; D025301:Hyphae / Q000166:cytology / Q000254:growth & development; D009154:Mutation; D010408:Penicillium chrysogenum / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D018086:Glutamate Dehydrogenase (NADP+)