title: Resveratrol metabolites in urine as a biomarker of wine intake in free-living subjects: The PREDIMED Study.
abstract: Several clinical and epidemiological studies have shown that moderate wine consumption may exert a protective effect against oxidative stress involved in several diseases, such as cardiovascular and neurodegenerative disorders. However, the epidemiological assessment of wine consumption has usually been obtained using self-reported questionnaires containing less reliable information for assessing total intake than nutritional biomarkers. A reliable biomarker for wine consumption is, therefore, needed. To validate urinary resveratrol metabolites (RMs) as a biomarker of wine consumption in a large cohort of free-living subjects, 1000 consecutive subjects entering a substudy of the PREDIMED trial (Prevención con Dieta Mediterránea) were evaluated. Data were collected in a validated semiquantitative food frequency questionnaire. RMs were measured in morning urine by LC-MS/MS. Urinary RM values correlated directly with reported daily amounts of wine consumed (r=0.895; p<0.001). One drink of wine per week can be detected. Using a cut-off of 411.4 nmol/g creatinine, the measurement of urinary RMs could discriminate wine consumers from non-wine consumers with a sensitivity of 93.3% (95% confidence interval (CI) 91.5-94.7%) and a specificity of 92.1% (CI 90.2-93.7%). Urinary RMs fulfill the criteria to be considered as a nutritional biomarker of wine consumption in a large sample of free-living subjects. This biomarker would provide an additional tool for investigating more precisely the relationship between wine consumption and health benefits.
authors.affiliation: Nutrition and Food Science Department-XaRTA, INSA, Pharmacy School, University of Barcelona, 08028 Barcelona, Spain.
authors.forename: Raul
authors.initials: R
authors.lastname: Zamora-Ros
authors.forename: Mireia
authors.initials: M
authors.lastname: Urpí-Sardà
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Lamuela-Raventós
authors.forename: Ramon
authors.initials: R
authors.lastname: Estruch
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Martínez-González
authors.forename: Mònica
authors.initials: M
authors.lastname: Bulló
authors.forename: Fernando
authors.initials: F
authors.lastname: Arós
authors.forename: Antonio
authors.initials: A
authors.lastname: Cherubini
authors.forename: Cristina
authors.initials: C
authors.lastname: Andres-Lacueva
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking / Q000453:epidemiology* / Q000378:metabolism / Q000652:urine*; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D002853:Chromatography, Liquid; D015331:Cohort Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000378:metabolism* / Q000652:urine*; D011795:Surveys and Questionnaires; D053719:Tandem Mass Spectrometry; D014920:Wine
chemical_list: D015415:Biomarkers; D013267:Stilbenes; D000077185:Resveratrol