title: Protection by Nigella sativa against carbon tetrachloride-induced downregulation of hepatic cytochrome P450 isozymes in rats.
abstract: Nigella sativa (family Ranunculaceae) is an annual plant that has been traditionally used on the Indian subcontinent and in Middle Eastern countries. In this study, we investigated the effect of N. sativa oil on the drug-metabolizing cytochrome P450 (CYP) enzymes and whether it has a protective effect against the acute hepatotoxicity of CCl4. Intraperitoneal injection of rats with CCl4 drastically decreased CYP2E1, CYP2B, CYP3A2, CYP2C11, and CYP1A2 mRNA and protein expressions. Oral administration of 1 ml/kg N. sativa oil every day for one week prior to CCl4 injection alleviated CCl4-induced suppression of CYP2B, CYP3A2, CYP2C11, and CYP1A2. Moreover, CCl4 increased iNOS and TNFalpha mRNA, while N. sativa oil administration for one week prior to CCl4 injection downregulated the CCl4-induced iNOS mRNA and up-regulated IL-10 mRNA. These results indicate that N. sativa oil administration has a protective effect against the CCl4-mediated suppression of hepatic CYPs and that this protective effect is partly due to the downregulation of NO production and up-regulation of the anti-inflammatory IL-10.
authors.affiliation: Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Zein S
authors.initials: ZS
authors.lastname: Ibrahim
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishizuka
authors.forename: Mohamed
authors.initials: M
authors.lastname: Soliman
authors.forename: Khlood
authors.initials: K
authors.lastname: ElBohi
authors.forename: Wageh
authors.initials: W
authors.lastname: Sobhy
authors.forename: Kaampwe
authors.initials: K
authors.lastname: Muzandu
authors.forename: Azza M
authors.initials: AM
authors.lastname: Elkattawy
authors.forename: Kentaro Q
authors.initials: KQ
authors.lastname: Sakamoto
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fujita
mesh_terms: D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy / Q000201:enzymology / Q000517:prevention & control*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D031881:Nigella sativa / Q000737:chemistry*; D008517:Phytotherapy; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats
chemical_list: D007527:Isoenzymes; D010938:Plant Oils; D016753:Interleukin-10; D003577:Cytochrome P-450 Enzyme System