title: Using marine natural products to discover a protease that catalyzes peptide macrocyclization of diverse substrates.
abstract: Small N-C terminally cyclic ribosomal peptides are common in nature, yet the mechanisms underlying their biosyntheses remain largely unknown. We recently identified candidate peptide cyclase genes in the metagenomes of marine animals. Here we report that PatG, a protease discovered in this analysis, cleaves variables, short peptides out of a precursor protein, and cyclizes these peptides in vivo and in vitro.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: Jaeheon
authors.initials: J
authors.lastname: Lee
authors.forename: John
authors.initials: J
authors.lastname: McIntosh
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Hathaway
authors.forename: Eric W
authors.initials: EW
authors.lastname: Schmidt
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D000458:Cyanobacteria / Q000201:enzymology; D047028:Macrocyclic Compounds / Q000737:chemistry* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000378:metabolism; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001688:Biological Products; D047028:Macrocyclic Compounds; D010456:Peptides, Cyclic; D011498:Protein Precursors; D012269:Ribosomal Proteins; D010447:Peptide Hydrolases