title: Down-regulatory effect of alpha 1-acid glycoprotein on bovine neutrophil degranulation.
abstract: In this paper, the possible involvement of the acute phase protein alpha(1)-acid glycoprotein (AGP) in the local immunomodulation of inflammation was investigated. The dose response of bovine neutrophils to AGP as to mobilization of primary and secondary granules was studied. It was found that AGP fulfils a protective role against spontaneous exocytosis of secondary, but not primary, granules. This downregulatory effect is much more evident when degranulation is challenged with Zymosan activated serum (ZAS). AGP activity is dose-dependent, the acute phase concentration being more active than the physiological one. Carbohydrate moiety of AGP was found to be critical, since experimentally desialylated protein does not maintain its exocytosis-modulatory activity. The fact that AGP may modulate the degranulation of neutrophils confirms the hypothesis that AGP is heavily involved in the fine tuning of neutrophil activity in the inflammatory environment.
authors.affiliation: Animal Pathology and Veterinary Public Health Department, University of Milan, Via Celoria 10, 20133 Milan, Italy.
authors.forename: Alba
authors.initials: A
authors.lastname: Miranda-Ribera
authors.forename: Cristina
authors.initials: C
authors.lastname: Lecchi
authors.forename: Valerio
authors.initials: V
authors.lastname: Bronzo
authors.forename: Licia
authors.initials: L
authors.lastname: Scaccabarozzi
authors.forename: Paola
authors.initials: P
authors.lastname: Sartorelli
authors.forename: Federica
authors.initials: F
authors.lastname: Franciosi
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ceciliani
mesh_terms: D000818:Animals; D002417:Cattle; D015550:Cell Degranulation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D007150:Immunohistochemistry; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D009961:Orosomucoid / Q000494:pharmacology*
chemical_list: D009961:Orosomucoid