Can splenocytes enhance pancreatic beta-cell function and mass in 90% pancreatectomized rats fed a high fat diet?
OBJECTIVE
Recent studies have shown that splenocytes may act as a possible neogenic source with regard to beta-cells in rodent diabetic models. Accordingly, we sought to determine whether splenocytes played an important role in promoting beta-cell function and mass among type 2 diabetic rats with and without spleen.


METHODS
We randomly divided female 90% pancreatectomized (Px) Sprague Dawley rats into three groups: splenectomy (SPX), splenectomy plus the injection of male splenocytes (SPI), and no splenectomy (NSP). They were administered with 40 energy percent fat diets over the course of five weeks. At the end of the experimental period, insulin secretion capacity was measured by hyperglycemic clamp. At 6 h after BrdU(+) injection, the pancreas was prepared with 4% paraformaldehyde in order to perform immunohistochemistry.


RESULTS
SPX increased and sustained serum glucose levels more than NSP and SPI during oral glucose tolerance testing. During hyperglycemic clamp, first and second phase insulin secretion decreased in the SPX rats while splenocyte injections counteracted this. Beta-cell mass in the SPX group was reduced more than among NSP and SPI. This was the result of a decrease in the number of small beta-cell clusters in SPX, which is indicative of a decrease in beta-cell neogenesis.


CONCLUSIONS
Splenocytes play an important role with regard to the neogenesis of beta-cells in insulin deficient type 2 diabetic rats, although they are not critical for beta-cell regeneration.
Park
Sunmin
S
Dept of Food and Nutrition, Institute of Basic Science, College of Natural Science, Hoseo University, Asan-Si 336-795, South Korea. smpark@hoseo.edu
Hong
Sang Mee
SM
Ahn
Il Sung
IS
D000818:Animals; D048429:Cell Size; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010180:Pancreatectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000502:physiology*; D013156:Splenectomy
D004041:Dietary Fats