Determination of 20(S)-ginsenoside Rh1 and its aglycone 20(S)-protopanaxatriol in rat plasma by sensitive LC-APCI-MS method and its application to pharmacokinetic study.
Liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) method has been developed for the measurement of the concentrations of 20(S)-ginsenoside Rh1 and its aglycone 20(S)-protopanaxatriol in rat plasma with panaxatriol as internal standard. The method involved single liquid-liquid extraction of both 20(S)-ginsenoside Rh1 and 20(S)-protopanaxatriol from plasma samples with n-butanol. The limit of quantification (LOQ) was 5 ng mL(-1) for both compounds. The method was validated within the linear range 5-2000 ng.mL(-1) for both compounds. The correlation coefficient for the calibration regression line was 0.999 or better. Intra-day and inter-day accuracy were better than 15%. The method has been successfully used for the pharmacokinetic studies in rats. After intravenous administrations, the mean retention times of 20(S)-ginsenoside Rh1 and 20(S)-protopanaxatriol were 17.1 +/- 2.0 min and 3.46 +/- 0.33 h, respectively.
Lai
Li
L
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
Liu
Yitong
Y
Hao
Haiping
H
Wang
Guangji
G
Chen
Xijing
X
Ren
Hongcan
H
D000818:Animals; D001274:Atmospheric Pressure; D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data; D002853:Chromatography, Liquid / Q000379:methods / Q000706:statistics & numerical data; D036145:Ginsenosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D013058:Mass Spectrometry / Q000379:methods / Q000706:statistics & numerical data; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012502:Sapogenins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity
D036145:Ginsenosides; D012502:Sapogenins; C081552:protopanaxatriol; C425564:ginsenoside Rh1