Speciation of inorganic arsenic in a sequential injection dual mini-column system coupled with hydride generation atomic fluorescence spectrometry.
The separation and speciation of inorganic arsenic(III) and arsenic(V) are facilitated by employing a novel sequential injection system incorporating two mini-columns followed by detection with hydride generation atomic fluorescence spectrometry. An octadecyl immobilized silica mini-column is used for selective retention of the complex between As(III) and APDC, while the sorption of As(V) is readily accomplished by a 717 anion exchange resin mini-column. The retained As(III)-PDC complex and As(V) are effectively eluted with a 3.0 mol L(-1) hydrochloric acid solution as stripping reagent, which well facilitates the ensuing hydride generation process via reaction with tetrahydroborate. With a sampling volume of 1.0 mL and an eluent volume of 100 microL for both species, linear ranges of 0.05-1.5 microg L(-1) for As(III) and 0.1-1.5 microg L(-1) for As(V) are obtained, along with enrichment factors of 7.0 and 8.2, respectively. Precisions of 2.8% for As(III) and 2.9% for As(V) are derived at the concentration level of 1.0 microg L(-1). The practical applicability of the procedure has been demonstrated by analyzing a certified reference material of riverine water (SLRS-4), in addition to spiking recovery in a lake water sample matrix.
Chen
Mingli
M
Research Center for Analytical Sciences, Northeastern University, Shenyang 110004, China.
Huo
Yumei
Y
Wang
Jianhua
J
D001151:Arsenic / Q000032:analysis* / Q000737:chemistry; D004867:Equipment Design; D005618:Fresh Water / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013054:Spectrophotometry, Atomic; D014873:Water Pollutants / Q000032:analysis
D014873:Water Pollutants; D001151:Arsenic