Bioactive borosilicate glass scaffolds: in vitro degradation and bioactivity behaviors.
Bioactive borosilicate glass scaffolds with the pores of several hundred micrometers and a competent compressive strength were prepared through replication method. The in vitro degradation and bioactivity behaviors of the scaffolds have been investigated by immersing the scaffolds statically in diluted phosphate solution at 37 degrees C, up to 360 h. To monitor the degradation progress of the scaffolds, the amount of leaching elements from the scaffolds were determined by ICP-AES. The XRD and SEM results reveal that, during the degradation of scaffolds, the borosilicate scaffolds converted to hydroxyapatite. The compressive strength of the scaffolds decreased during degradation, in the way that can be well predicted by the degradation products, or the leachates, from the scaffolds. MTT assay results demonstrate that the degradation products have little, if any, inhibition effect on the cell proliferation, when diluted to a certain concentration ([B] <2.690 and pH value at neutral level). The study shows that borosilicate glass scaffold could be a promising candidate for bone tissue engineering material.
Liu
Xin
X
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai, China.
Huang
Wenhai
W
Fu
Hailuo
H
Yao
Aihua
A
Wang
Deping
D
Pan
Haobo
H
Lu
William W
WW
Jiang
Xinquan
X
Zhang
Xiuli
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001673:Biodegradation, Environmental; D001854:Bone Marrow Cells / Q000166:cytology; D018786:Bone Substitutes / Q000737:chemistry*; D001881:Borates / Q000737:chemistry; D002478:Cells, Cultured; D019245:Compressive Strength; D004577:Electron Probe Microanalysis; D005898:Glass / Q000737:chemistry*; D006041:Goats; D006863:Hydrogen-Ion Concentration; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010710:Phosphates / Q000737:chemistry; D016062:Porosity; D011208:Powders; D017640:Silicates / Q000737:chemistry*; D012996:Solutions; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D018786:Bone Substitutes; D001881:Borates; D010710:Phosphates; D011208:Powders; D017640:Silicates; D012996:Solutions