title: Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
abstract: We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.
authors.affiliation: Laboratory of Stem Cell Biology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Gil
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Woo
authors.forename: Joong-Hyun
authors.initials: JH
authors.lastname: Shim
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: You
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Park
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Paek
authors.forename: Suel-Kee
authors.initials: SK
authors.lastname: Kim
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Kim
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D055495:Neurogenesis; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D019096:Vitronectin / Q000378:metabolism*; D000099176:Noggin Protein
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D019096:Vitronectin; D000099176:Noggin Protein