[FTIR spectroscopic explorations of freshly resected laryngeal carcinoma tissues].
OBJECTIVE
To investigate the characteristics of freshly resected laryngeal carcinoma by Fourier transform infrared spectroscopy (FTIR).


METHODS
FTIR was applied to the study of the cancerous tissues and adjacent normal tissues in 32 patients.


RESULTS
Compared with pathological diagnosis results, one benign specimen was classified as a malignant, the accuracy was 98.4%. Significant differences were seen in the FTIR spectra between the normal and malignant laryngeal tissues. The peak at 1085 cm(-1) shift to 1114 cm(-1) showed that the relative contents of DNA in laryngeal carcinoma cells was increased. The peak at 1397 cm(-1) was stronger than 1451 cm(-1) in normal tissues, while it was not obvious in cancer tissues. I(2926)/I(2870) in carcinoma cells was lower than that in normal tissues. The wave numbers of the bands of amide I and amide II, symmetric and asymmetric stretching bands of CH(3), stretching vibration bands of C-OH and NH band were shifted to higher number in cancer tissues.


CONCLUSIONS
The study shows that the malignant and normal laryngeal tissues have different FTIR spectra, which are mainly due to changes in protein, nucleic acid and phospholipids. FTIR may become a new method for the diagnosis of laryngeal carcinoma in clinical practice.
Wu
Zheng-Hu
ZH
Department of Otorhinolaryngology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China. wutt168@hotmail.com
Cui
Cai-Xia
CX
Liu
Ting-Yan
TY
Jiang
Jian-Hua
JH
Zhou
Xue-Hua
XH
Pan
Qing-Hua
QH
D006801:Humans; D007822:Laryngeal Neoplasms / Q000737:chemistry* / Q000175:diagnosis* / Q000473:pathology; D007830:Larynx / Q000737:chemistry* / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis*; D009696:Nucleic Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D009363:Neoplasm Proteins; D009696:Nucleic Acids; D010743:Phospholipids