[Effects of leptin on porcine primary adiocytes lipolysis and mRNA expression of key lipolytic enzymes].
Leptin, a cytokine predominantly secreted from fat tissue, plays an important role in regulating organism energy balance. Leptin can stimulate lipolysis, but the mechanism is unclear. In order to study the molecular mechanism of leptin stimulating lipolysis, we systemically studied the mRNA expression of key lipolytic enzymes. Morphological observation, Oil Red O staining and RT-PCR were used to identify pig primary adipocytes; commercial kits were used to measure the glycerol and FFA release; Semiquantitative RT-PCR was used to detect the mRNA expression of key lipolytic enzymes. The results showed that 100 nmol/L leptin up-regulated the mRNA expression of ATGL, TGH-2, HSL, MGL and LPL (P<0.01), but down-regulated the Perilipin mRNA expression (P<0.01). At the same time, leptin promoted the glycerol release in a dose dependent manner (P<0.01), but had no effect on the FFA release (P>0.05). These indicate that leptin may mainly stimulate lipolysis in pig primary adipocytes by up-regulating the expression of ATGL, MGL, LPL and down-regulating the expression of Perilipin. The unchanged FFA release may be resulted from Leptin promoting UCPs mRNA expression and increasing FFA expenditure.
Li
Yucheng
Y
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Sciences and Technology, Northwest A & F University, Yangling 712100, China.
Zheng
Xueli
X
Yang
Gongshe
G
D017667:Adipocytes / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D020738:Leptin / Q000494:pharmacology*; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D008994:Monoacylglycerol Lipases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine
D020738:Leptin; D012333:RNA, Messenger; D008994:Monoacylglycerol Lipases; D008049:Lipase