Fluorescence signal amplification by cation exchange in ionic nanocrystals.
Lighting-up time: A cation-exchange reaction releases thousands of divalent cations from nonfluorescent CdSe ionic crystals and triggers fluorescence from thousands of originally nonfluorescent Fluo-4 fluorophores to obtain a large fluorescence amplification (see picture) and a low detection limit in bioassays. The technique is fast, simple, with a large dye-to-reporter labeling ratio, and flexible in selection of nanocrystals and fluorophores.
Li
Jishan
J
Department of Chemistry, University of California, Riverside, CA 92521, USA.
Zhang
Tierui
T
Ge
Jianping
J
Yin
Yadong
Y
Zhong
Wenwan
W
D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry; D001681:Biological Assay; D019187:Cadmium Compounds / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry*; D003460:Crystallization; D005453:Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014966:Xanthenes / Q000138:chemical synthesis / Q000737:chemistry
D000814:Aniline Compounds; D019187:Cadmium Compounds; D002413:Cations, Divalent; C409648:Fluo 4; D018036:Selenium Compounds; D014966:Xanthenes; C058667:cadmium selenide