title: Drosophila Past1 is involved in endocytosis and is required for germline development and survival of the adult fly.
abstract: Endocytosis, which is a key process in eukaryotic cells, has a central role in maintaining cellular homeostasis, nutrient uptake, development and downregulation of signal transduction. This complex process depends on several protein-protein interactions mediated by specific modules. One such module is the EH domain. The EH-domain-containing proteins comprise a family that includes four vertebrate members (EHD1-EHD4) and one Drosophila ortholog, Past1. We used Drosophila as a model to understand the physiological role of this family of proteins. We observed that the two predicted Past1 transcripts are differentially expressed both temporally and spatially during the life cycle of the fly. Endogenous Past1 as well as Past1A and Past1B, expressed from plasmids, were localized mainly to the membrane of Drosophila-derived cells. We generated mutants in the Past1 gene by excising a P-element inserted in it. The Past1 mutants reached adulthood but died precociously. They were temperature sensitive and infertile because of lesions in the reproductive system. Garland cells that originated from Past1 mutants exhibited a marked decrease in their ability to endocytose fluorescently labeled avidin. Genetic interaction was found between Past1 and members of the Notch signaling pathway, suggesting a role for Past1 in this developmentally crucial signaling pathway.
authors.affiliation: Department of Cell Research and Immunology, Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, 69978, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Olswang-Kutz
authors.forename: Yaron
authors.initials: Y
authors.lastname: Gertel
authors.forename: Sigi
authors.initials: S
authors.lastname: Benjamin
authors.forename: Orly
authors.initials: O
authors.lastname: Sela
authors.forename: Olga
authors.initials: O
authors.lastname: Pekar
authors.forename: Eli
authors.initials: E
authors.lastname: Arama
authors.forename: Hermann
authors.initials: H
authors.lastname: Steller
authors.forename: Mia
authors.initials: M
authors.lastname: Horowitz
authors.forename: Daniel
authors.initials: D
authors.lastname: Segal
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D004331:Drosophila melanogaster / Q000196:embryology; D004705:Endocytosis; D005260:Female; D005455:Fluorescent Antibody Technique; D017353:Gene Deletion; D015870:Gene Expression; D007246:Infertility / Q000378:metabolism*; D008136:Longevity; D008297:Male; D008969:Molecular Sequence Data; D009866:Oogenesis; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D013079:Sperm Maturation; D013312:Stress, Physiological
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D029721:Drosophila Proteins; C497759:N protein, Drosophila; C496522:Past1 protein, Drosophila; D051880:Receptors, Notch