title: Photocrosslinkable chitosan hydrogel can prevent bone formation in both rat skull and fibula bone defects.
abstract: UV light irradiation to a photocrosslinkable chitosan (Az-CH-LA) resulted in an insoluble and flexible hydrogel within 30 s. The purpose of this study was to evaluate the ability of the photocrosslinkable chitosan to inhibit bone formation in the bone defects. A 5-mm-diameter defect was made in the rat calvarium, and then photocrosslinkable chitosan was implanted and irradiated with UV for 30 s. Furthermore, a 2-mm defect was made in the fibula of a rat hind leg, and then photocrosslinkable chitosan was implanted and irradiated with UV. Bone formations in the rat skull and fibula defects with photocrosslinkable chitosan hydrogel were significantly prevented for 8 weeks. Thus, the chitosan hydrogel has an inhibitory effect on bone formation.
authors.affiliation: Department of Orthopaedic Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Amako
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Arino
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yura
authors.forename: Koichi
authors.initials: K
authors.lastname: Nemoto
mesh_terms: D000818:Animals; D048271:Chitosan / Q000494:pharmacology* / Q000528:radiation effects; D005360:Fibula / Q000187:drug effects*; D020100:Hydrogels / Q000494:pharmacology* / Q000528:radiation effects; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects*; D014466:Ultraviolet Rays
chemical_list: D020100:Hydrogels; D048271:Chitosan