[Emergency prophylactic effects of the avian influenza virus immunized serum on the infected mice].
OBJECTIVE
To evaluate emergency prophylactic effects of the avian influenza virus immunized serum on experimentally infected mice.


METHODS
Serum HI antibody titers of 30 mice were detected at day 1 to 19 after being inoculated with 0.2 ml immune serum to estimate half life of immune serum. Ten mice clinical symptom was recorded to estimate the serum security after mice injected 1.5 ml immune serum. Seventy mice were randomly divided into 7 groups according to random number table and inoculated with 0.2 ml, 0.1 ml and 0.05 ml immune serum respectively via intraperitoneal injection on day 8, 4 and 1 prior to challenged with 10 LD(50) influenza virus intranasal. Mice were observed continually for 14 days to calculate the morbidity, mortality, average survival days and compare the lung index and viral titers in lung.


RESULTS
Serum HI antibody titers of mice which inoculated with 0.2 ml immune serum maintained 2(6) in 15 days after injection, but drawdown after day 17, the mice injected 1.5 ml immune serum were all alive and none onset. The survival rate of mice which injected 0.2 ml serum on the day 8, 4, 1 before challenge was 80%, 100% and 100%, and the average survival period was 13.1 days, 14.0 days and 14.0 days respectively. The survival rate of mice which injected 0.1 ml and 0.05 ml serum on day 1 before challenge was 100% and 50%, and the average survival days were 14.0 days and 11.7 days respectively. The mice lung index of experimental groups (0.0096 +/- 0.0033 - 0.0145 +/- 0.0060) was smaller than that of viral control group (0.0199 +/- 0.0025), with a statistical significance (P value 0.0022 - 0.0470, < 0.05). The viral titers in lung were significantly decreased by 2 titer as compared to the viral controls.


CONCLUSIONS
The avian influenza virus immunized serum might contain the emergency prophylactic effects and could be developed as an agent for possible human-avian influenza pandemic.
Wang
Cheng-yu
CY
Virology Laboratory, the Military Veterinary Institute, Academy of Military Medical Sciences of PLA, Changchun 130062, China.
Wang
Hua-lei
HL
Feng
Na
N
Yang
Song-tao
ST
Gao
Yu-wei
YW
Wang
Tei-cheng
TC
Zou
Xiao-huan
XH
Xia
Xian-zhu
XZ
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009976:Orthomyxoviridae Infections / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera