title: Carrier-state of two or three polymorphic variants of MTHFR, IL-6 and ICAM1 genes increases the risk of coronary artery disease.
abstract: BACKGROUND
  Progression of coronary artery disease (CAD) depends on multiple genetic and environmental factors. Polymorphic variants of genes encoding proteins involved in endothelium dysfunction or proinflammatory state may genetically differentiate the human population and determine a susceptibility to the disease.
  
  
  OBJECTIVE
  To find the relationship between single polymorphisms of MTHFR, IL-6 and ICAM1 genes, double or triple combinations among their polymorphic variants and CAD in a patient population from the Upper Silesia region.
  
  
  METHODS
  The study population consisted of 177 patients with angiographically documented CAD and 202 blood donors with no signs of CAD. All examined individuals were white Polish Caucasians aged 18-55 years. The analysis of genetic polymorphisms was performed using the PCR-RFLP method.
  
  
  RESULTS
  We observed a weak association between single gene polymorphism and the disease only in the case of the MTHFR T allele. We also found that the frequency of some double or triple combinations among analysed genes, especially for MTHFR+ICAM1 and MTHFR+ICAM1+IL-6 patterns, differentiated the entire patient group from controls (p=0.047 OR=1.75 and p=0.016 OR=1.75, respectively). These two combinations were also significantly more frequent in patients who were age-matched with controls, especially in the female subgroups (for MTHFR+ICAM1 p=0.007, OR=10.32 and for MTHFR+ ICAM1+IL-6, p=0.005, OR=17.95 in females).
  
  
  CONCLUSIONS
  The present study showed that simultaneous carrier-state of MTHFR, IL-6 and ICAM1 genes increased the risk of CAD.
authors.affiliation: Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland. beatasarecka@poczta.onet.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Sarecka-Hujar
authors.forename: Iwona
authors.initials: I
authors.lastname: Zak
authors.forename: Jolanta
authors.initials: J
authors.lastname: Krauze
mesh_terms: D000328:Adult; D003327:Coronary Disease / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006579:Heterozygote; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP)