title: Comparative study of the cytotoxic effect of Resilon against two cell lines.
abstract: Resilon is a new material that is a candidate to replace gutta-percha as a root filling material. This study evaluated the antiproliferative effect of Resilon and two commercially available gutta-percha points (Roeko, Dentsply). Two established cell lines (L929 and RPC-C2A) were used for the experiment. Cell survival fraction was estimated by the sulforhodamine-B assay, in reference to controls after 48-h exposure. Non-parametric tests (Kruskal-Wallis followed by Dunn's multiple comparisons) were used to evaluate the statistical significance of the results (alpha=0.05). Cytotoxicity in a descending order was: Resilon > Roeko gutta-percha > Dentsply gutta-percha. At 24-h exposure, no statistically significant differences (p>0.05) were observed between tested materials in both cell lines. At 48-h exposure, statistically significant differences (p<0.05) were found between Resilon and the other materials in the L929 cell line. In the RPC-C2A cell line Resilon was significantly more cytotoxic than Dentsply gutta-percha (p<0.05), but no statistically significant differences (p>0.05) were found between Resilon and Roeko gutta-percha. The cytotoxicity of Resilon increased significantly from 24 h to 48 h in both cell lines. Resilon points were more cytotoxic than gutta-percha points. The cytotoxicity was time dependent and increased after 48 h.
authors.affiliation: Department of Endodontology, Dental School, Aristotle University, Thessaloniki, Greece.
authors.forename: Nickolaos
authors.initials: N
authors.lastname: Economides
authors.forename: Elisabeth A
authors.initials: EA
authors.lastname: Koulaouzidou
authors.forename: Christos
authors.initials: C
authors.lastname: Gogos
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kolokouris
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Beltes
authors.forename: Demetrios
authors.initials: D
authors.lastname: Antoniades
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006171:Gutta-Percha / Q000633:toxicity; D007739:L Cells; D051379:Mice; D051381:Rats; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013997:Time Factors
chemical_list: D012387:Root Canal Filling Materials; C486892:resilon sealer; D006171:Gutta-Percha