title: A rapid MEKC method for the simultaneous determination of creatinine, 1- and 3-methylhistidine in human urine.
abstract: The present report describes the use of MEKC in the presence of 35 mM sodium tetraborate, pH 9.0, containing 60 mM SDS for the complete separation and identification of creatinine, 1-methylhistidine (1-MeH) and 3-MeH in human urine. Their simultaneous quantification in urine of healthy controls and subjects submitted to elective surgery to their lower limbs allowed to use 3-MeH as a reliable measure of skeletal protein breakdown. Simplicity, sensitivity and low running costs are the main advantages of this method that is suitable for the routine analysis in clinical laboratories of a large number of samples per day.
authors.affiliation: Department of Biochemistry A. Castellani, University of Pavia, Pavia, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Ferrari
authors.forename: Marco
authors.initials: M
authors.lastname: Fumagalli
authors.forename: Simona
authors.initials: S
authors.lastname: Viglio
authors.forename: Roberto
authors.initials: R
authors.lastname: Aquilani
authors.forename: Evasio
authors.initials: E
authors.lastname: Pasini
authors.forename: Paolo
authors.initials: P
authors.lastname: Iadarola
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003404:Creatinine / Q000652:urine*; D006801:Humans; D008762:Methylhistidines / Q000652:urine*; D009124:Muscle Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D008762:Methylhistidines; D009124:Muscle Proteins; C028120:1-methylhistidine; D003404:Creatinine; C028118:3-methylhistidine