title: X-ray crystal structure of GarR-tartronate semialdehyde reductase from Salmonella typhimurium.
abstract: Tartronate semialdehyde reductases (TSRs), also known as 2-hydroxy-3-oxopropionate reductases, catalyze the reduction of tartronate semialdehyde using NAD as cofactor in the final stage of D-glycerate biosynthesis. These enzymes belong to family of structurally and mechanically related beta-hydroxyacid dehydrogenases which differ in substrate specificity and catalyze reactions in specific metabolic pathways. Here, we present the crystal structure of GarR a TSR from Salmonella typhimurium determined by the single-wavelength anomalous diffraction method and refined to 1.65 A resolution. The active site of the enzyme contains L-tartrate which most likely mimics a position of a glycerate which is a product of the enzyme reaction. The analysis of the TSR structure shows also a putative NADPH binding site in the enzyme.
authors.affiliation: Biosciences Division, Midwest Center for Structural Genomics, Argonne National Laboratory, Bldg 202, Argonne, IL 60439, USA.
authors.forename: J
authors.initials: J
authors.lastname: Osipiuk
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: Moy
authors.forename: F
authors.initials: F
authors.lastname: Collart
authors.forename: A
authors.initials: A
authors.lastname: Joachimiak
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D012645:Selenomethionine; D000429:Alcohol Oxidoreductases; C064167:2-hydroxy-3-oxopropionate reductase