Current techniques for the study of troponin I phosphorylation in human heart.
Cardiac troponin I phosphorylation has been shown to be reduced in human heart failure and hypertrophic cardiomyopathy using the increasingly popular Pro-Q Diamond phosphoprotein gel stain. In this brief report I discuss the use of Western immunoblotting, non-equilibrium isoelectric focussing and Pro-Q Diamond and introduce phosphate affinity SDS-PAGE using Phos-tag-acrylamide for the investigation of troponin I phosphorylation.
Gallon
Clare E
CE
BHF Laboratories, Division of Medicine, University College London, Rayne Building, 5 University Street, London, WC1E 6JF, UK. c.gallon@ucl.ac.uk
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism*; D010766:Phosphorylation; D019210:Troponin I / Q000378:metabolism*
D019210:Troponin I