title: [Simultaneous determination of four glycosylflavones from Lophatherum gracile by RP-HPLC].
abstract: OBJECTIVE
  To develop a RP-HPLC method for simultaneous determination of orientin, isorientin, vitexin and isovitexin in Lophatherum gracile from different habitat and harvesting time.
  
  
  METHODS
  The HPLC method was applied and the chromatographic column was a Waters XBridge C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase consisted of methanol-0.05% acetic acid (35:65). The flow rate was 1.0 mL min(-1) and the detection wavelength was set at 340 nm. The column temperature was set at 25 degrees C.
  
  
  RESULTS
  Four components were isolated well, the linear relationships were excellent. The mean recoveries and RSD values of orientin, isorientin, vitexin and isovitexin were 103.2%, 2.1%; 101.6%, 2.7%; 98.4%, 2.3%; 99.2%, 1.8%, respectively.
  
  
  CONCLUSIONS
  The HPLC method is simple, sensitive and reliable, and can be used for the quality control of the medicinal material.
authors.affiliation: Research and Development Certer of Natural Medicine, Zhejiang Forestry University, Lin'an 311300, China. yuan_ke001@163.com
authors.forename: Ke
authors.initials: K
authors.lastname: Yuan
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Xue
authors.forename: Ming-Wen
authors.initials: MW
authors.lastname: Yin
authors.forename: Lu-Huan
authors.initials: LH
authors.lastname: Lou
mesh_terms: D047310:Apigenin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; D047310:Apigenin; C032731:vitexin; C065886:orientin; C049772:isovitexin