title: Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer.
abstract: Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.
authors.affiliation: Department of Urology, University of California at San Francisco, San Francisco, CA 94115, USA. pparis@cc.ucsf.edu
authors.forename: Pamela L
authors.initials: PL
authors.lastname: Paris
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.forename: Shivaranjani
authors.initials: S
authors.lastname: Sridharan
authors.forename: Tina
authors.initials: T
authors.lastname: Fan
authors.forename: Howard L
authors.initials: HL
authors.lastname: Adler
authors.forename: Emmanuel R
authors.initials: ER
authors.lastname: Yera
authors.forename: M H
authors.initials: MH
authors.lastname: Zarrabi
authors.forename: Stanley
authors.initials: S
authors.lastname: Zucker
authors.forename: Jeffry
authors.initials: J
authors.lastname: Simko
authors.forename: Wen-Tien
authors.initials: WT
authors.lastname: Chen
authors.forename: Jonathan
authors.initials: J
authors.lastname: Rosenberg
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D009919:Orchiectomy; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor