Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2.
Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.
Piontek
Christian
C
Bioorganische Chemie, Gebäude NW1, Universität Bayreuth, 95440 Bayreuth, Germany.
Varón Silva
Daniel
D
Heinlein
Christian
C
Pöhner
Claudia
C
Mezzato
Stefano
S
Ring
Petra
P
Martin
Andreas
A
Schmid
Franz Xaver
FX
Unverzagt
Carlo
C
D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D004722:Endoribonucleases / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D004798:Enzymes / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D008026:Ligation; D017510:Protein Folding; D020673:Protein Renaturation
D002241:Carbohydrates; D004798:Enzymes; D006020:Glycopeptides; D006023:Glycoproteins; D004722:Endoribonucleases; C029493:ribonuclease C