[Expression and its significance of aquaporin 5 in nasal polyps with or without allergic rhinitis].
OBJECTIVE
To confirm the expression and distribution of aquaporin 5 (AQP5) in nasal polyps.


METHODS
AQP5 were studied in 20 samples nasal polyp without AR (Group NAR) and 16 samples with AR (Group AR) with immunochemistric staging and its integrated light intensity and its grey step.


RESULTS
(1) Hematoxylin and eosin staining showed that there was obvious inflammation reaction, a large quantity of glands hyperplasia and acidophils soaked in nasal polyps, especially those with allergic rhinitis. (2) Immunofluorescence technique showed that the distribution of AQP5 in the two groups was in accordance with that in nasal polyps on the whole. AQP5 expressed mainly in the membrane and cytoplasm of the epithelium of the glands, ducts and cilia. (3) The statistical analysis of the immunohistochemical staining showed that the integrated light intensity of AQP5 in Group AR (0.1675+/-0.006536) was obviously higher than that in the Group NARs nasal polyps (0.09343+/-0.001816). There was statistical significance between the two groups (t = -12.00, P<0.01). (4) The statistical analysis of the immunohistochemical staining showed that the grey step of AQP5 in Group AR (175.6+/-2.471) was obviously lower than that in the Group NAR's nasal polyps (206.2+/-0.9649). There was statistical significance between the two groups (t=12.46, P<0.01).


CONCLUSIONS
AQP5 is expressed in nasal polyp. Combined with the research before, it was conferred that the hypersecretion of glands and its character has a relationship with the high expression of AQP5 in nasal polyps, and nasal polyp with different symptom has different mechanism.
Jiang
Yuan
Y
Department of Otolaryngology, Drum Tower Clinic Medical College of Nanjing Medical University, Nanjing, 210029, China.
Yu
Chenjie
C
Shen
Xiaohui
X
Wang
Jingmeit
J
Gao
Xia
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D051402:Aquaporin 5 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D009298:Nasal Polyps / Q000150:complications / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000150:complications / Q000378:metabolism*; D055815:Young Adult
C496036:AQP5 protein, human; D051402:Aquaporin 5