Tools to investigate reaction oxygen species-sensitive signaling proteins.
The thiol groups ofcysteine residues on proteins are attractive oxidative targets for modification by reactive oxygen species, such as hydrogen peroxide (H2O2). Such modification can lead to important cellular signaling processes that ultimately result in modification of the physiology of the organism. To identify such proteins that are amenable to oxidative modification, different methods can be used. Here, two such approaches are described: one being the use of fluorescent thiol derivatives, and the second being the use of genetic mutants that are mutated in thiol residues. Using the model plant Arabidopsis thaliana, cell cultures, and whole plants, we describe these tools to help the reader understand the function of such thiol modifications on plant responses.
Desikan
Radhika
R
Division of Biology, Imperial College London, London, UK.
Neill
Steven J
SJ
Slinn
Jenna
J
Hancock
John T
JT
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004586:Electrophoresis; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000037:antagonists & inhibitors; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008967:Molecular Biology / Q000379:methods*; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling
D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)