Electrophoretic field gradient focusing with on-column detection by fluorescence quenching.
Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.
Ansell
Richard J
RJ
School of Chemistry, University of Leeds, Leeds, UK. chmrja@leeds.ac.uk
Tuñón
Pilar González
PG
Wang
Yating
Y
Myers
Peter
P
Ivory
Cornelius F
CF
Keen
Jeffrey N
JN
Findlay
John B C
JB
D000818:Animals; D004574:Electromagnetic Fields; D004867:Equipment Design; D005456:Fluorescent Dyes; D007525:Isoelectric Focusing / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis*
D005456:Fluorescent Dyes; D011506:Proteins