title: Care regimen and lens material influence on silicone hydrogel contact lens deposition.
abstract: OBJECTIVE
  To quantitatively detect proteins and cholesterol extracted from worn silicone hydrogel contact lenses and determine the effect of various lens care solutions on deposit accumulation.
  
  
  METHODS
  Contact lenses, made from different polymers and worn on a daily wear schedule with different lens care solutions, were collected. Lipid and protein deposits were extracted by methanol:chloroform (1:1, v/v) and protein extraction solution (containing urea and surfactant), respectively. Lipid extracts were separated and cholesterol quantified using thin layer chromatography. Protein extracts were quantified using standard techniques.
  
  
  RESULTS
  Among all lenses tested, Balafilcon A lenses exhibited greatest extracted cholesterol (4.1 to 8.2 microg/lens) and total protein (5.4 to 23.2 microg/lens). AQuify was the most effective solution in reducing extracted deposits, especially extracted protein, from Balafilcon A lenses. AQuify and Opti-Free RepleniSH solutions were most effective in reducing extracted cholesterol from Senofilcon A and Galyfilcon A lenses, respectively. Use of Opti-Free Express solution resulted in more extracted protein from Lotrafilcon B lenses than use of other solutions. Generally, Lotrafilcon B, Senofilcon A, and Galyfilcon A lenses accumulated relatively low amount of proteins. Lotrafilcon B lenses accumulated the least amount of cholesterol deposit among all lenses tested regardless of solution used.
  
  
  CONCLUSIONS
  Lens polymer (possibly associated with surface characteristics) is a prominent factor affecting lipid and protein accumulation. Within a lens polymer type, lens care solutions exhibit varying effectiveness in reducing protein and lipid accumulation.
authors.affiliation: Institute for Eye Research, Sydney, Australia. z.zhao@ier.org.au
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Zhao
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Carnt
authors.forename: Yulina
authors.initials: Y
authors.lastname: Aliwarga
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Wei
authors.forename: Thomas
authors.initials: T
authors.lastname: Naduvilath
authors.forename: Qian
authors.initials: Q
authors.lastname: Garrett
authors.forename: John
authors.initials: J
authors.lastname: Korth
authors.forename: Mark D P
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis*; D018639:Contact Lens Solutions / Q000494:pharmacology* / Q000592:standards; D003263:Contact Lenses, Hydrophilic; D005136:Eye Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels / Q000737:chemistry; D012828:Silicones / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D018639:Contact Lens Solutions; D005136:Eye Proteins; D020100:Hydrogels; D012828:Silicones; C523195:balafilcon A; C524582:lotrafilcon B; C523196:senofilcon A; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002784:Cholesterol