A unique plasma proteomic profiling with imbalanced fibrinogen cascade in patients with Kawasaki disease.
Kawasaki disease (KD) is the leading cause of acquired heart disease during childhood in the developed countries. The mechanism and biomarkers of KD remain to be determined. In this study, we sought to elucidate potential plasma proteomic markers in KD patients in comparison to that in febrile controls. Plasma samples from KD patients and febrile controls were subjected to two-dimensional polyacrylamide gel electrophoresis analysis. Differential protein displays between KD patients and febrile controls were determined. Fibrinogen beta and gamma chains, alpha-1-antitrypsin (A1AT), CD5 antigen-like precursor (CD5L), and clusterin were increased in KD patients, whereas immunoglobulin free light chains were decreased, as compared with controls. The differential protein displays were validated with enzyme-linked immunosorbent assay tests. We found higher fibrinogen-related proteins (fibrinogen, A1AT, clusterin, and CD5L), along with a lower level of the immunoglobulin free light chains that involve fibrin degradation in KD. Results from this study showing a unique proteomic profiling with abnormal fibrinogen cascade may afford a good biomarker of KD and a better strategy to prevent cardiovascular complications of KD by correcting abnormal fibrin deposition or degradation.
Yu
Hong-Ren
HR
Department of Pediatrics, Chang Gung Memorial Hospital - Kaohsiung Medical Center, Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Taiwan.
Kuo
Ho-Chang
HC
Sheen
Jiunn-Ming
JM
Wang
Lin
L
Lin
I-Chun
IC
Wang
Chih-Lu
CL
Yang
Kuender D
KD
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000378:metabolism*; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009080:Mucocutaneous Lymph Node Syndrome / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D001798:Blood Proteins; D020543:Proteome; D005340:Fibrinogen