Physical exercise increases mitochondrial function and reduces oxidative damage in skeletal muscle.
The present study investigated mitochondrial adaptations and oxidative damage after 4 and 8 weeks of running training in skeletal muscle of mice. Twenty-one male mice (CF1, 30-35 g) were distributed into the following groups (n = 7): untrained (UT); trained-4 weeks (T4); trained-8 weeks (T8). Forty-eight hours after the last training session the animals were killed by decapitation and quadriceps (red portion) were removed and stored at -70 degrees C. Succinate dehydrogenase (SDH), complexes I, II, II-III and IV, lipoperoxidation (TBARS), protein carbonyls (PC) and total thiol content were measured. Results show that endurance training (8-wk) increases the SDH activity and complexes (I, II, III, IV), decreases oxidative damage (TBARS, CP) and increases total thiol content in skeletal muscle when compared to untrained animals. In conclusion, eight weeks of running training are necessary for increases in mitochondrial respiratory chain enzyme activities to occur, in association with decreased oxidative damage.
Silva
Luciano A
LA
Laboratório de Fisiologia e Bioquímica do Exercício, UNESC, Bairro Universitário, 88806-000, Criciúma, SC, Brazil. luciano_acordi@yahoo.com.br
Pinho
Cleber A
CA
Scarabelot
Karoline S
KS
Fraga
Daiane B
DB
Volpato
Ana M J
AM
Boeck
Carina R
CR
De Souza
Cláudio T
CT
Streck
Emilio L
EL
Pinho
Ricardo A
RA
D000818:Animals; D004579:Electron Transport / Q000502:physiology; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008931:Mitochondria, Muscle / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D050050:Protein Carbonylation / Q000502:physiology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D013438:Sulfhydryl Compounds; D013385:Succinate Dehydrogenase