A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences.
BACKGROUND
Although hepatitis C virus (HCV) genotype 6 is classified into 21 subtypes, 6a-6u, new variants continue to be identified.


OBJECTIVE
To characterize the full-length genomes of three novel HCV genotype 6 variants: KMN02, KM046 and KM181.


METHODS
From sera of patients with HCV infection, the entire HCV genome was amplified by RT-PCR followed by direct DNA sequencing and phylogenetic analysis.


RESULTS
The sera contained HCV genomes of 9461, 9429, and 9461nt in length, and each harboured a single ORF of 9051nt. The genomes showed 95.3-98.1% nucleotide similarity to each other and 72.2-75.4% similarity to 23 genotype 6 reference sequences, which represent subtypes 6a-6u and unassigned variants km41 and gz52557. Phylogenetic analyses demonstrated that they were genotype 6, but were subtypically distinct. Based on the current criteria of HCV classification, they were designed to represent a new subtype, 6v. Analysis of E1 and NS5B region partial sequences revealed two additional related variants, CMBD-14 and CMBD-86 that had been previously reported in northern Thailand and sequences dropped into Genbank.


CONCLUSIONS
Three novel HCV genotype 6 variants were entirely sequenced and designated subtype 6v.
Wang
Yizhong
Y
Sun Yat-sen University, Guangzhou, Guangdong, China.
Xia
Xueshan
X
Li
Chunhua
C
Maneekarn
Niwat
N
Xia
Wenjie
W
Zhao
Wenhua
W
Feng
Yue
Y
Kung
Hsiang Fu
HF
Fu
Yongshui
Y
Lu
Ling
L
D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D044967:Serum / Q000821:virology; D013785:Thailand
D012367:RNA, Viral