[The emergence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis isolated from sporadic patients in Zhejiang province].
OBJECTIVE
To identify the presence of candidate pathogenicity island 89K DNA sequence of Streptococcus suis serotype 2 (SS2) strains isolated from patient in Zhejiang province.


METHODS
Genes and DNA fragments were amplified by PCR, using specific primers, and three amplified fragments of the 89K sequence were directly sequenced. The results were analyzed using software related to bioinformatics and epidemiology.


RESULTS
8 strains of SS2 all contained 89K sequence, cps2J and mrp virulent genes, and species-specific 16S rDNA. 3 amplified fragments of 89K candidate pathogenicity island of SS2 ZJ0501 were above 99% similar to SS2 strain identified from outbreaks in Jiangsu in 1998, and the gene fragment of coding DNA recombinant protein in the 89K sequence was highly homological with that of S. dysgalactiae and S.agalactiae.


CONCLUSIONS
In recent years SS2 strains isolated from patients with clinical symptoms in Zhejiang province had been detected to have contained candidate pathogenic 89K DNA fragment.
Zhu
Shui-rong
SR
The Institute of Microbiology, Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China.
Wang
Zhi-gang
ZG
Chen
En-fu
EF
Yang
Ting-ting
TT
Zhang
Zheng
Z
Xu
Bao-xiang
BX
Jin
Da-zhi
DZ
He
Jian-qin
JQ
Wang
Fu-su
FS
D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000235:genetics; D044404:Genomic Islands; D005838:Genotype; D006801:Humans; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000453:epidemiology / Q000235:genetics*; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial