Kinetic study on the binding of lectin to mannose residues in a polymer brush.
A disulfide-carrying polymer with many pendent mannose residues (dithiolated poly(2-methacryloyloxyethyl D-mannopyranoside)) was obtained by atom transfer radical polymerization (ATRP) method using 2-(2'-bromoisobutyroyloxy)ethyl disulfide as initiator. The obtained disulfide-carrying glycopolymer (DT-PMEMan) was accumulated as a polymer brush on a colloidal gold-immobilized glass substrate, and the binding processes of a lectin, Concanavalin A (Con A), to mannose residues in the polymer brush were examined using a UV-vis spectrophotometer with the help of a localized surface plasmon resonance. Con A showed a concentration-dependent specific binding to the glycopolymer brush, and the apparent association constant of Con A with mannose residues in the brush was much larger than the association constant for small sugars due to the so-called cluster effect. Furthermore, the glycopolymer brush-coated device had a detection limit lower than 5 nM. The glycopolymer-carrying device examined here is expected to expand our knowledge of recognition phenomena at the surface of polymer brushes.
Kitano
Hiromi
H
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan. kitano@eng.u-toyama.ac.jp
Takahashi
Yoshiko
Y
Mizukami
Kazuya
K
Matsuura
Kazuhiro
K
D000042:Absorption; D002241:Carbohydrates / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D005898:Glass; D006046:Gold; D007700:Kinetics; D037102:Lectins / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D013379:Substrate Specificity
D002241:Carbohydrates; D003102:Colloids; D004220:Disulfides; D037102:Lectins; D011108:Polymers; D003208:Concanavalin A; D006046:Gold; D008358:Mannose