Quantitative matrix-assisted laser desorption ionization-fourier transform ion cyclotron resonance (MALDI-FT-ICR) peptide profiling and identification of multiple-sclerosis-related proteins.
We introduce a matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).
Stoop
Marcel P
MP
Laboratories of Neuro-Oncology/Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam 3000 DR, The Netherlands.
Dekker
Lennard J
LJ
Titulaer
Mark K
MK
Lamers
Robert-Jan A N
RJ
Burgers
Peter C
PC
Sillevis Smitt
Peter A E
PA
van Gool
Alain J
AJ
Luider
Theo M
TM
Hintzen
Rogier Q
RQ
D000595:Amino Acid Sequence; D017357:Cyclotrons; D005583:Fourier Analysis; D006801:Humans; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D010455:Peptides / Q000134:cerebrospinal fluid*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides; D020543:Proteome