The effect of a layer-by-layer chitosan-heparin coating on the endothelialization and coagulation properties of a coronary stent system.
A biomacromolecular layer-by-layer coating process of chitosan/heparin onto a coronary stent is designed for the acceleration of the re-endothelialization and healing process after coronary stent deployment. The results of in vitro culturing of porcine iliac artery endothelial cells as well as the measurements of the APTT, PT and TT supported the rationale that the combination of chitosan and heparin could bring both endothelial cell compatibility and haemocompatibility to the stent surface. A porcine coronary injury model and arteriovenous shunt model were used for the further evaluation of the application of this kind of surface-modified stainless steel stent in vivo. The final results proved that this facile coating approach could significantly promote re-endothelialization and was safer compared with bare metal stents for its much improved anticoagulation property.
Meng
Sheng
S
Department of Macromolecular Science and The Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Fudan University, Shanghai 200433, People's Republic of China.
Liu
Zongjun
Z
Shen
Li
L
Guo
Zhang
Z
Chou
Laisheng L
LL
Zhong
Wei
W
Du
Qiangguo
Q
Ge
Junbo
J
D000818:Animals; D001777:Blood Coagulation; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003331:Coronary Vessels / Q000166:cytology* / Q000293:injuries; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology*; D006493:Heparin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D015607:Stents; D013552:Swine
D006493:Heparin; D048271:Chitosan