title: [AZF microdeletions are not related with recurrent spontaneous abortion].
abstract: OBJECTIVE
  To study the relationship between azoospermia factor (AZF) microdeletions of the Y-chromosome and recurrent spontaneous abortion.
  
  
  METHODS
  We collected 26 chorionic villus samples from abortive male embryos and 51 blood samples from the husbands whose wives had recurrent spontaneous abortion, extracted genomic DNA from the samples and detected 12 STSs in the AZFa, AZFb and AZFc regions of Yq11.2 by amplification multiplex PCR.
  
  
  RESULTS
  AZF microdeletions were found neither in the chorionic villus samples nor in the blood samples.
  
  
  CONCLUSIONS
  There is no relationship between AZF microdeletions and recurrent spontaneous abortion.
authors.affiliation: PLA Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. lu_lhy@163.com
authors.forename: Hong-Yong
authors.initials: HY
authors.lastname: Lu
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Cui
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Xia
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Ge
authors.forename: Bing
authors.initials: B
authors.lastname: Yao
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000022:Abortion, Spontaneous / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D005260:Female; D056426:Genetic Loci; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D029589:Seminal Plasma Proteins / Q000235:genetics*
chemical_list: D029589:Seminal Plasma Proteins