An investigation of bioactive phytochemicals in the leaves of Melicope vitiflora by electrospray ionisation ion trap mass spectrometry.
The ethyl acetate extract of the leaves of Melicope vitiflora was separated by column chromatography and the resulting fractions tested for their bioactivity towards methicillin-resistant-Staphylococcus aureus (MRSA) and Micrococcus luteus (ML). The bioactive column chromatography fractions were further separated by preparative TLC and dereplication was carried out on them by first subjecting them to electrospray ionisation-ion trap mass spectrometry (ESI-MS(n)). The resulting molecular masses, their fragmentation patterns in addition to the chemnet database (www.chemnetbase.com) were used to aid in the structural elucidation of some of the compounds by permitting comparison with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multi drug resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for M. vitiflora.
O'Donnell
F
F
School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine, Co. Derry BT52 1SA, Northern Ireland, United Kingdom.
Ramachandran
V N
VN
Smyth
T J P
TJ
Smyth
W F
WF
Brooks
P
P
D000900:Anti-Bacterial Agents / Q000032:analysis; D002855:Chromatography, Thin Layer; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D027821:Rutaceae / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012822:Silicon Dioxide