Expression of corticosteroid-binding globulin in human astrocytoma cell line.
Glial tumor cells are known to be sensitive to glucocorticoids (GC) in vivo and in vitro. Here we studied the expression of corticosteroid-binding globulin (CBG) in the low-grade malignant human astrocytoma cell line 1321N1. CBG was observed in cytoplasm of most of these cells with immunocytochemistry. RT-PCR revealed the presence of the respective mRNA. Only scattered cells contained nuclear immunoreactivity for glucocorticoid receptor as visualized by double immunostaining. Immunoreactive CBG could be recovered from the supernatant of cultures that had been exposed to 10(-5) M cortisol. Our observations indicate the endogenous expression of CBG in 1321N1 cells which may occur independently from classical glucocorticoid receptor pathways. Cortisol seems to facilitate liberation of CBG in a paracrine manner, perhaps through membrane action of the steroid. Effects of adrenal steroids on proliferation and apoptosis of certain glial tumors may in part depend on these mechanisms.
Pusch
Larissa
L
Inst für Anatomie II, Friedrich Schiller Universität Jena, Jena, Germany.
Wegmann
Sonja
S
Caldwell
Jack D
JD
Jirikowski
Gustav F
GF
D001254:Astrocytoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D003907:Dexamethasone / Q000378:metabolism; D005938:Glucocorticoids / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D014156:Transcortin / Q000235:genetics / Q000378:metabolism*
D005938:Glucocorticoids; D007328:Insulin; C497713:NR3C1 protein, human; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D014156:Transcortin; D006854:Hydrocortisone