[Determination of 1-deoxynojirimycin in Morus alba L. leaves using reversed-phase high performance liquid chromatography fluorescence detection with pre-column derivatization].
A rapid, reliable and suitable method for the determination of 1-deoxynojirimycin (DNJ) in Morus alba L. leaves has been developed. The DNJ in 100 mg dried leaves was extracted twice with 10 mL aqueous 0.05 mol/L HCl, then derivatized by 6-aminoquinoiyi-N-hydroxysuccinimidyl carbamate (AQC) in K3BO3 buffer (pH 8.5), and analyzed using a high performance liquid chromatograph equipped with a fluorescence detector. The analyte was eluted with a mobile phase of 0.02 mol/L potassium dihydrogen phosphate buffer-acetonitrile (85: 15, v/v) at the rate of 1.0 mL/min. The derivatized DNJ was well dissolved from the hydrolysis products of AQC. The linearity ranged from 0.5 to 25 mg/L, and the detection limit was 0.02 mg/L (S/N = 3). The content of DNJ in Morus alba L. leaves was 0.12%, the recovery was 96.1%-98.6%.
Xie
Huiming
H
Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China.
Wu
Fangrui
F
Yang
Yi
Y
Liu
Jie
J
D017485:1-Deoxynojirimycin / Q000032:analysis* / Q000737:chemistry*; D002138:Calibration; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D017485:1-Deoxynojirimycin