title: Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.
abstract: The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, 48824, USA.
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Vasileiou
authors.forename: Kin Sing Stephen
authors.initials: KS
authors.lastname: Lee
authors.forename: Rachael M
authors.initials: RM
authors.lastname: Crist
authors.forename: Soheila
authors.initials: S
authors.lastname: Vaezeslami
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Goins
authors.forename: James H
authors.initials: JH
authors.lastname: Geiger
authors.forename: Babak
authors.initials: B
authors.lastname: Borhan
mesh_terms: D001120:Arginine / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D018698:Glutamic Acid / Q000235:genetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D018168:Receptors, Retinoic Acid / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism
chemical_list: D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular; D018698:Glutamic Acid; D014212:Tretinoin; D001120:Arginine