title: Application of physicochemically modified silicon substrates as reverse-phase protein microarrays.
abstract: Physicochemically modified silicon substrates can provide a high quality alternative to nitrocellulose-coated glass slides for use in reverse-phase protein microarrays. Enhancement of protein microarray sensitivities is an important goal, especially because molecular targets within patient tissues exist in low abundance. The ideal array substrate has a high protein binding affinity and low intrinsic background signal. Silicon, which has low intrinsic autofluorescence, is being explored as a potential microarray surface. In a previous paper ( Nijdam , A. J. ; Cheng , M. M.-C. ; Fedele , R. ; Geho , D. H. ; Herrmann , P. ; Killian , K. ; Espina , V. ; Petricoin , E. F. ; Liotta , L. A. ; Ferrari , M. Physicochemically Modified Silicon as Substrate for Protein Microarrays . Biomaterials 2007 , 28 , 550 - 558 ), it is shown that physicochemical modification of silicon substrates increases the binding of protein to silicon to a level comparable with that of nitrocellulose. Here, we apply such substrates in a reverse-phase protein microarray setting in two model systems.
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA. nijdam@gwu.edu
authors.forename: A Jasper
authors.initials: AJ
authors.lastname: Nijdam
authors.forename: Michael R
authors.initials: MR
authors.lastname: Zianni
authors.forename: Edward E
authors.initials: EE
authors.lastname: Herderick
authors.forename: Mark M-C
authors.initials: MM
authors.lastname: Cheng
authors.forename: Jenifer R
authors.initials: JR
authors.lastname: Prosperi
authors.forename: Fredika A
authors.initials: FA
authors.lastname: Robertson
authors.forename: Emanuel F
authors.initials: EF
authors.lastname: Petricoin
authors.forename: Lance A
authors.initials: LA
authors.lastname: Liotta
authors.forename: Mauro
authors.initials: M
authors.lastname: Ferrari
mesh_terms: D000418:Albumins / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000418:Albumins; D012825:Silicon