The use of Cyan Fluorescent Protein variants with a distinctive lifetime signature.
The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel.
Kim
Jiho
J
Institut Pasteur Korea, 39-1 Hawolgok-dong, Seoul 136-791, South Korea.
Kwon
DoYoon
D
Lee
Joohyun
J
Pasquier
Hélène
H
Grailhe
Regis
R
D000818:Animals; D006224:Cricetinae; D019020:Directed Molecular Evolution; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D009154:Mutation; D015202:Protein Engineering / Q000379:methods; D013696:Temperature; D013997:Time Factors
C485182:Cyan Fluorescent Protein; D049452:Green Fluorescent Proteins