[Evaluation of the inhibitory effect of tripeptide tyroservatide on growth of human hepatocellular carcinoma using a hollow fiber assay].
OBJECTIVE
To investigate the inhibitory effect of tyroservatide (YSV) on growth of hepatocellular carcinoma cells.


METHODS
In vitro effects of YSV on five human hepatocellular carcinoma cell lines were assayed by MTS. In vivo effects of YSV on 5 human hepatocellular carcinoma cell lines were assayed by hollow fiber tumor model.


RESULTS
After treatment with YSV at a dose of 0.1 approximately 1.6 mg/ml, the growth of the five cell lines was significantly inhibited in vitro compared with that of the control group (P < 0.05). Especially, YSL remarkably inhibited the growth of human hepatocellular carcinoma BEL-7402 cells, i.e. the cell growth was inhibited by 63.3% after treatment with YSL at 1.6 mg/ml. The hollow fiber tumor model demonstrated that YSL (320 microg x kg(-1) x d(-1) and 640 microg x kg(-1) x d(-1)) treatment significantly inhibited the in vivo growth of the five cancer cell lines compared with that in the saline control (P < 0.05). YSL showed the highest level of inhibition of human BEL-7402 hepatocellular carcinoma cells, with an inhibitory index of 53.1% at 320 microg x kg(-1) x d(-1).


CONCLUSIONS
As a new method, hollow fiber assay may be used to evaluate the inhibitory effect of drugs on different tumor cells in vivo, rapidly, accurately and economically. Our results provide an instruction and evidence for clinical use of YSV.
Shi
Lin-xi
LX
Department of Immunology, Tianjin Medical University, Tianjin 300070, China.
Lu
Rong
R
Wang
Li
L
Zhou
Chun-lei
CL
Xu
Qiong
Q
Zhu
Zhi-feng
ZF
Ma
Rui
R
Zhang
Hua-li
HL
Jia
Jing
J
Fu
Zheng
Z
Yao
Zhi
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000494:pharmacology*; D011897:Random Allocation
D000970:Antineoplastic Agents; D009842:Oligopeptides; C510683:tripeptide tyroservatide