title: [Determination of benomyl, carbendazim and thiabendazole in apple juice concentrate using solid-phase extraction coupled with ion exchange chromatography].
abstract: A method was developed for the determination of benomyl, carbendazim and thiabendazole in apple juice concentrate by solid-phase extraction coupled with ion exchange chromatography (IEC). The sample was diluted with water, and then benomyl was degradated completely to carbendazim at 80 degrees C, and purified by an SCX solid-phase extraction column. Liquid chromatographic analysis was performed on the instrument of Agilent 1200 series equipped with a diode-array detector and autosampler. The column was LC-SCX (25 cm x 4.6 mm, 5 microm). The mobile phase was 0.1 mol/L KH2PO4 (pH 2.5)-acetonitrile (70:30, v/v) with a flow rate of 1.0 mL/min. The presented method showed good linear relationship with good precision and accuracy at the range of 0.02 - 2.0 mg/L for carbendazim and thiabendazole. The detection limits were 0. 004 mg/kg for carbendazim and thiabendazole. The method was characterized with acceptable sensitivity to meet the requirements for monitoring these pesticides in apple juice concentrate.
authors.affiliation: Shaanxi Entry-Exit Inspection and Quarantine Bureau, Xi' an 710068, China. heq@snciq.gov.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Xianghong
authors.initials: X
authors.lastname: Kong
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Aishan
authors.initials: A
authors.lastname: Yue
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001542:Benomyl / Q000032:analysis / Q000302:isolation & purification; D001562:Benzimidazoles / Q000032:analysis / Q000302:isolation & purification; D001628:Beverages / Q000032:analysis*; D002219:Carbamates / Q000032:analysis / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis*; D016014:Linear Models; D027845:Malus / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013696:Temperature; D013827:Thiabendazole / Q000032:analysis / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D010573:Pesticide Residues; C006698:carbendazim; D013827:Thiabendazole; D001542:Benomyl