Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells.
The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.
Mvula
B
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Laser Research Group, Faculty of Health Science, University of Johannesburg, Doornfontein, Johannesburg, 2028, South Africa.
Moore
T J
TJ
Abrahamse
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology; D053687:Adult Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D049449:Luminescence; D018800:Thy-1 Antigens / Q000378:metabolism
D015415:Biomarkers; D018800:Thy-1 Antigens; D004815:Epidermal Growth Factor; D000255:Adenosine Triphosphate