title: Dietary protein content influences both growth and size distribution of anterior and posterior muscle fibres in juveniles of Pagellus bogaraveo (Brunnich).
abstract: Muscle cellularity was studied in Pagellus bogaraveo juveniles fed on diets with different protein contents. Measured in transversal body sections, at both post-opercular and post-anal locations, the morphometric variables estimated were: total muscle area (A), total number of fibres (N), number of fibres per unit area of muscle (N (A)) and cross-sectional fibre area (ā) of the two main muscle fibre types. At the end of the experiment fish fed on diets having more than 40% of protein displayed significantly higher body weight. Fish fed on protein-rich diets exhibited greater ā and N. For fish fed on 30 and 50% protein diets the morphometric parameters measured grew linearly with the fish weight. High-protein diets favoured muscle hyperplasia. When comparing rostral and caudal locations, a greater N and a smaller ā of posterior red fibres were the consistent differences found-a fact, to our knowledge, so far unreported for fish.
authors.affiliation: Laboratory of Histology and Embryology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Silva
authors.forename: L M P
authors.initials: LM
authors.lastname: Valente
authors.forename: M H
authors.initials: MH
authors.lastname: Galante
authors.forename: C A P
authors.initials: CA
authors.lastname: Andrade
authors.forename: R A F
authors.initials: RA
authors.lastname: Monteiro
authors.forename: E
authors.initials: E
authors.lastname: Rocha
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D049628:Body Size; D004044:Dietary Proteins / Q000008:administration & dosage*; D024510:Muscle Development; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000187:drug effects; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects*; D018657:Muscle Fibers, Slow-Twitch / Q000166:cytology / Q000187:drug effects; D010473:Perciformes / Q000033:anatomy & histology* / Q000254:growth & development*
chemical_list: D004044:Dietary Proteins