title: Sequence-specific electrochemical detection of Alicyclobacillus acidoterrestris DNA using electroconductive polymer-modified fluorine tin oxide electrodes.
abstract: This study outlines the quantification of low levels of Alicyclobacillus acidoterrestris in pure cultures, since this bacterium is not inactivated by pasteurization and may remain in industrialized foods and beverages. Electroconductive polymer-modified fluorine tin oxide (FTO) electrodes and multiple nanoparticle labels were used for biosensing. The detection of A. acidoterrestris in pure cultures was performed by reverse transcription polymerase chain reaction (RT-PCR) and the sensitivity was further increased by asymmetric nested RT-PCR using electrochemical detection for quantification of the amplicon. The quantification of nested RT-PCR products by Ag/Au-based electrochemical detection was able to detect 2 colony forming units per mL (CFU mL(-1)) of spores in pure culture and low detection and quantification limits (7.07 and 23.6 nM, respectively) were obtained for the target A. acidoterrestris on the electrochemical detection bioassay.
authors.affiliation: GMEME - Grupo de Materiais Eletroquímicos e Métodos Eletroanalíticos, Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense, 400, C.P. 780, 13560-970, São Carlos - SP, Brazil.
authors.forename: Katlin Ivon
authors.initials: KI
authors.lastname: Barrios Eguiluz
authors.forename: Giancarlo Richard
authors.initials: GR
authors.lastname: Salazar-Banda
authors.forename: Maribel Elizabeth
authors.initials: ME
authors.lastname: Funes-Huacca
authors.forename: Juliana Vieira
authors.initials: JV
authors.lastname: Alberice
authors.forename: Emanuel
authors.initials: E
authors.lastname: Carrilho
authors.forename: Sergio Antonio
authors.initials: SA
authors.lastname: Spinola Machado
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Avaca
mesh_terms: D001406:Bacillaceae / Q000235:genetics*; D001628:Beverages / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005461:Fluorine; D005516:Food Microbiology; D005638:Fruit; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017971:Tin Compounds
chemical_list: D004269:DNA, Bacterial; D017971:Tin Compounds; D005461:Fluorine; C045358:stannic oxide