[Identification of porcine parvovirus and virus-specific antibodies by polymerase chain reaction and enzyme immunoassay].
Porcine parvovirus infection (PPVI) caused by a small non-enveloped virus (porcine parvovirus, PPV) is responsible for serious reproductive dysfunctions in sows. In the Russian Federation, the approved diagnostic methods for determination of parvovirus antigen and virus-specific antibodies are hemagglutination and hemagglutination-inhibition tests, respectively. The present paper gives the results of developing diagnostic tests to reveal porcine parvovirus by polymerase chain reaction and its antibodies by recombinant protein-based indirect enzyme immunoassay. The developed tests supplement a battery of diagnostic methods and specify some disputable results obtained by classical methods.
Eliseeva
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000379:methods*; D010322:Parvoviridae Infections / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000821:virology
D000914:Antibodies, Viral