[Identification of Gynostemma from their adulterant Cayratia japonica by PCR-RFLP].
OBJECTIVE
To establish a convenient and effective method for the identification of Gynostemma and Cayratia japonica.


METHODS
Eight species, including Gynostemm pentaphyllum, G. pentagynum, G. cardiospermum, G. longipe, G. yixingense, G. laxiflorum, G. guangxiense and C. japonica were investigated through PCR - RFLP of six chloroplast DNA fragments. The six gene fragments were digested by six restriction endonuclease respectively, including Taq I, Hpa II, EcoR I, Rsa I, Hha I, Hind III.


RESULTS
Seven species of Gynostemma and their adulterant could be identified by trnK1f-trnK2r and Rsa.


CONCLUSIONS
PCR - RFLP provides a quick, reliable molecular marker technique for identification of Cynostemma and their adulterant Cayratia japonica.
Wang
Chong
C
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.
Zhang
Yuan-Yuan
YY
Yang
Juan
J
Jiang
Ling-Yan
LY
Li
Miao-Miao
MM
Zhao
Gui-Fang
GF
D018742:DNA, Chloroplast / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D029774:Gynostemma / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D027844:Vitaceae / Q000145:classification* / Q000235:genetics*
D018742:DNA, Chloroplast; D018744:DNA, Plant