Transcriptional activity of interferon gamma and two subunits of its receptor as molecular markers of myocarditis.
Inflammatory cytokines have an important role in the pathogenesis of myocarditis, but still little is known about the importance of interferon gamma (IFNg) in this disease. The aim of the study was to evaluate the prognostic value of the initial transcriptional activity of IFNg and two subunits of its receptor as measured with the use of QRT-PCR and SYBRGreen chemistry in the group of 63 patients with clinically confirmed myocarditis who were treated with statin or immunosupressive therapy. The initial values of IFNg and the ratio of IFNgRb/IFNgRa were statistically different in the analyzed group of patients. The prognostic value of IFNg and IFNgRb/IFNgRa was determined by logistic regression analysis.
Smolik
Sławomir
S
Department of Biochemistry, Medical University of Silesia, 1 Narcyzów St., 41-200 Sosnowiec, Poland. epimer@wp.pl
Domal-Kwiatkowska
Dorota
D
Nowalany-Kozielska
Ewa
E
Wojnicz
Romuald
R
Swiatowska
Longina
L
Ludmiła
Weglarz
W
D052160:Benzothiazoles; D015415:Biomarkers / Q000378:metabolism; D003959:Diamines; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007371:Interferon-gamma / Q000235:genetics* / Q000378:metabolism; D016015:Logistic Models; D009205:Myocarditis / Q000175:diagnosis / Q000188:drug therapy / Q000503:physiopathology*; D009930:Organic Chemicals / Q000378:metabolism; D011379:Prognosis; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011804:Quinolines; D017471:Receptors, Interferon / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D000096743:Interferon gamma Receptor
D052160:Benzothiazoles; D015415:Biomarkers; D003959:Diamines; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007166:Immunosuppressive Agents; D007371:Interferon-gamma; D009930:Organic Chemicals; D021122:Protein Subunits; D011804:Quinolines; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor; C098022:SYBR Green I