Protein recognition by an ensemble of fluorescent DNA G-quadruplexes.
Sniffing out proteins: Fluorescent DNA G-quadruplexes have been used for building versatile signaling receptors for proteins in a single solution. Introducing a protein sample to the ensemble results in a unique emission signature for unambiguous identification (see scheme, R = fluorophore). The self-assembled, pattern-based protein detection systems are easily fabricated, have the potential for high-throughput operations, and have the ability to handle small protein samples.
Margulies
David
D
Department of Chemistry, Yale University, P. O. Box 208107, New Haven, CT 06520-8107, USA.
Hamilton
Andrew D
AD
D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D054856:G-Quadruplexes; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D011506:Proteins; D004247:DNA