title: [Study on genetic relationship of official Rheum by SRAP].
abstract: OBJECTIVE
  To determine the genetic relationship of three species of official Rheum in molecular level.
  
  
  METHODS
  Twelve samples from three species of official Rheum were employed to be analyzed by the approach of sequence-related amplified polymorphism (SRAP). Systematic relationships were constructed based on the UPGMA method by TREECONW software.
  
  
  RESULTS
  A total of 272 bands were scored and 199 bands of them were polymorphic, which were up to 73.2% polymorphic ratio. Genetic similarity coefficient was changed from 0.578 4 to 0.941 6. The results indicated that there was abundant genetic diversity among the tested materials. The clustering analysis revealed that the results between SRAP marker and the traditional morphological characteristics was almost the same.
  
  
  CONCLUSIONS
  SRAP marker is suitable for variety identification and genetic relationship research in official Rheum.
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China. chendaxia6891@hotmail.com
authors.forename: Da-Xia
authors.initials: DX
authors.lastname: Chen
authors.forename: Long-Yun
authors.initials: LY
authors.lastname: Li
authors.forename: Guo-Yue
authors.initials: GY
authors.lastname: Zhong
authors.forename: Song-Yun
authors.initials: SY
authors.lastname: Qin
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Wang
authors.forename: Zai-Bo
authors.initials: ZB
authors.lastname: Yu
mesh_terms: D016000:Cluster Analysis; D014644:Genetic Variation / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012250:Rheum / Q000145:classification / Q000235:genetics*