Antimicrobial effect and resistant regulation of Glycyrrhiza uralensis on methicillin-resistant Staphylococcus aureus.
In the present study, we investigated antimicrobial activity of Glycyrrhiza uralensis against various strains of methicillin-resistant Staphylococcus aureus (MRSA) (KCCM 11812, 40510, 40512). Glycyrrhiza uralensis was extracted by 80% MeOH and fractionated by organic solutions. The extract and fractions showed antimicrobial activity against standard S. aureus as well as MRSA. In the minimum inhibitory concentration test, G. uralensis showed 0.25 mg mL(-1) in hexane fraction and 0.10-0.12 mg mL(-1) in chloroform fraction. Especially, chloroform fraction showed 2.5 times higher antimicrobial activity than penicillin. Furthermore, chloroform fraction correlated with MRSA gene expression (MecA, MecI, MecRI, FemA). These results suggest that G. uralensis may have potent antimicrobial activity and thus, this medicinal herb can be a suitable phytotherapeutic agent for treating MRSA infections.
Lee
Ji-Won
JW
Department of Food Science and Technology, Keimyung University, Daegu, Korea.
Ji
Young-Ju
YJ
Yu
Mi-Hee
MH
Bo
Mi-Hyang Hwang
MH
Seo
Hwa-Jeong
HJ
Lee
Sam-Pin
SP
Lee
In-Seon
IS
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D046915:Penicillin-Binding Proteins; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001426:Bacterial Proteins; C096686:FemA protein, Bacteria; C101640:MecI protein, Staphylococcus; D046915:Penicillin-Binding Proteins; D010936:Plant Extracts; D012333:RNA, Messenger; D012097:Repressor Proteins; C487789:mecA protein, Staphylococcus aureus; C101641:mecR1 protein, Staphylococcus aureus