Targeted lipidomics approach for endogenous N-acyl amino acids in rat brain tissue.
Great effort has been devoted to characterize signaling lipids in central nervous system. This has led to a search for novel strategies to characterize hitherto unknown lipid compositions. Here we developed two methods, one for identification and one for quantification, for N-acyl amino acids, a novel lipid family. The identification method contains a series of purification steps followed by nano-LC/MS/MS and high-throughput screening of the datasets with a potent search algorithm based on fragment ion analysis. MS/MS spectra with good quality can be obtained with 150 fmol of targeted lipids on column with our nano-LC/MS/MS. More than one thousand mass spectra generated using the information dependent acquisition mode of Analyst QS software can be analyzed in 1 min using our home built software. The quantification method utilized the multiple reaction monitoring mode in Analyst software to measure the endogenous levels of N-acyl amino acids in rat brain. Using these two methods we were able to identify and quantify 11 previously reported N-acyl amino acids with endogenous levels ranging from 0.26 to 333 pmol g(-1) wet rat brain.
Tan
Bo
B
The Gill Center for Biomolecular Science, Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, United States. tanbo76@hotmail.com
Yu
Y William
YW
Monn
M Francesca
MF
Hughes
H Velocity
HV
O'Dell
David K
DK
Walker
J Michael
JM
D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000596:Amino Acids; D008055:Lipids