title: Liquid chromatography-tandem mass spectrometry method for the simultaneous quantitative determination of the organophosphorus pesticides dimethoate, fenthion, diazinon and chlorpyrifos in human blood.
abstract: Simultaneous determination of the organophosphorus pesticides dimethoate, fenthion, diazinon and chlorpyrifos in human blood by HPLC-tandem mass spectrometry was developed and validated. The pesticides were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Luna C(18) (30mmx2.0mm, 3microm) column, using a step-gradient at a flow rate of 0.4ml/min. The assay was linear from 0.5 to 100ng/ml (r(2)>0.992, n=24) for all pesticides. The inter- and intra-day accuracy and precision for the method was 96.6-106.1% and <10%, respectively. The lower limit of quantification was 0.5ng/ml. In conclusion, the method described displays analytical performance characteristics that are suitable for the quantification of these pesticides in cases of acute poisoning.
authors.affiliation: Australian Bioanalytical Services Pty Ltd., Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia. psalm@austbioservices.com.au
authors.forename: Paul
authors.initials: P
authors.lastname: Salm
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Taylor
authors.forename: Darren
authors.initials: D
authors.lastname: Roberts
authors.forename: Janaka
authors.initials: J
authors.lastname: de Silva
mesh_terms: D000328:Adult; D004390:Chlorpyrifos / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D003976:Diazinon / Q000097:blood* / Q000506:poisoning; D004117:Dimethoate / Q000097:blood*; D005260:Female; D005284:Fenthion / Q000097:blood*; D006801:Humans; D010575:Pesticides / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010575:Pesticides; D005284:Fenthion; D004390:Chlorpyrifos; D004117:Dimethoate; D003976:Diazinon