Altered gene expression in cloned piglets.
Studies on cloned pigs are scant compared with those in mice and cattle. Expression profiles of cloned pig embryos on full-term cloned pigs are even more limited owing to the limited availability of DNA microarray technology in the pig. We have conducted expression profile comparisons between pigs from somatic cell nuclear transfer and pigs from conventional breeding at birth and 1 month of age. Differentially expressed genes that are subjected to DNA methylation were also examined for their DNA methylation status. These data will be presented in the 2009 Annual Meeting of the International Embryo Transfer Society in San Diego. In the present review, we focus on summarising existing findings on epigenetic and other changes in cloned embryo, cloned pigs and their offspring by conventional breeding.
Tian
X Cindy
XC
Department of Animal Science and Center for Regenerative Biology, University of Connecticut, Storrs, CT 06269, USA. xiuchun.tian@uconn.edu
Park
Joonghoon
J
Bruno
Richard
R
French
Richard
R
Jiang
Le
L
Prather
Randall S
RS
D000818:Animals; D019976:Cloning, Organism; D019175:DNA Methylation; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018392:Genomic Imprinting / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D034421:Sus scrofa; D016615:Telomere / Q000235:genetics