Quantitative analysis of peripheral tissue perfusion using spatiotemporal molecular dynamics.
BACKGROUND
Accurate measurement of peripheral tissue perfusion is challenging but necessary to diagnose peripheral vascular insufficiency. Because near infrared (NIR) radiation can penetrate relatively deep into tissue, significant attention has been given to intravital NIR fluorescence imaging.


RESULTS
We developed a new optical imaging-based strategy for quantitative measurement of peripheral tissue perfusion by time-series analysis of local pharmacokinetics of the NIR fluorophore, indocyanine green (ICG). Time-series NIR fluorescence images were obtained after injecting ICG intravenously in a murine hindlimb ischemia model. Mathematical modeling and computational simulations were used for translating time-series ICG images into quantitative pixel perfusion rates and a perfusion map. We could successfully predict the prognosis of ischemic hindlimbs based on the perfusion profiles obtained immediately after surgery, which were dependent on the preexisting collaterals. This method also reflected increases in perfusion and improvements in prognosis of ischemic hindlimbs induced by treatment with vascular endothelial growth factor and COMP-angiopoietin-1.


CONCLUSIONS
We propose that this novel NIR-imaging-based strategy is a powerful tool for biomedical studies related to the evaluation of therapeutic interventions directed at stimulating angiogenesis.
Kang
Yujung
Y
Department of Bio and Brain Engineering, KAIST, Daejeon, Korea.
Choi
Myunghwan
M
Lee
Jungsul
J
Koh
Gou Young
GY
Kwon
Kihwan
K
Choi
Chulhee
C
D000818:Animals; D003198:Computer Simulation; D006614:Hindlimb / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007208:Indocyanine Green / Q000493:pharmacokinetics*; D007511:Ischemia / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D008962:Models, Theoretical; D010477:Perfusion; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C515576:COMP-Ang1 fusion protein; D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; D007208:Indocyanine Green