title: The antigenotoxic activities of cactus (Opuntia ficus-indica) cladodes against the mycotoxin zearalenone in Balb/c mice: prevention of micronuclei, chromosome aberrations and DNA fragmentation.
abstract: Zearalenone (ZEN) is a potent estrogenic metabolite. Evidence of its cytotoxicity and genotoxicity has recently emerged from several reports. This study was conducted to evaluate the ability of cactus (Opuntia ficus-indica) cladodes to protect Balb/c mice against ZEN induced genotoxicity. To this end, the effect of a single dose of ZEN (40 mg/kg b.w.) alone and with extract of cactus cladodes (25, 50 and 100 mg/kg b.w.) was monitored by measuring: (i) micronuclei induction in bone marrow cells, (ii) chromosome aberrations mainly breaks and gaps in bone marrow cells also and finally and (iii) DNA fragmentation in liver and kidney. Our results clearly show that ZEN is genotoxic to Balb/c mice. It induces DNA damage as indicated by DNA fragmentation, micronuclei and chromosomal aberrations in bone marrow cells. It is of note that cactus cladodes extract assayed alone at high dose (100 mg/kg b.w.) was found completely safe and did not induce any genotoxic effects. The simultaneous administration of cactus cladodes extract with ZEN resulted in an efficient prevention of micronuclei (the number of PCE MN decreased from 71.3+/-6.1 for animals treated with Zen to 32.6+/-15.5 for animals treated with cactus cladodes), chromosomal aberrations frequency (the % of chromosomal aberrations decreased from 38.3+/-3.0 to 18.6+/-1.1) in bone marrow cells and of DNA fragmentation compared to the group treated with ZEN alone. It could be concluded that cactus cladodes extract was effective in the protection against ZEN genotoxicity. This could be relevant, particularly with the emergent demand for natural products which may neutralize the genotoxic effects of the multiple food contaminants.
authors.affiliation: Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia.
authors.forename: Lazhar
authors.initials: L
authors.lastname: Zorgui
authors.forename: Imen
authors.initials: I
authors.lastname: Ayed-Boussema
authors.forename: Yosra
authors.initials: Y
authors.lastname: Ayed
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
authors.forename: Wafa
authors.initials: W
authors.lastname: Hassen
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology* / Q000633:toxicity*; D002869:Chromosome Aberrations; D053938:DNA Fragmentation; D004587:Electrophoresis, Agar Gel; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D029747:Opuntia / Q000737:chemistry*; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D016587:Antimutagenic Agents; D015025:Zearalenone