title: Hamster zona pellucida is formed by four glycoproteins: ZP1, ZP2, ZP3, and ZP4.
abstract: The zona pellucida (ZP) is an extracellular glycoprotein matrix that surrounds all mammalian oocytes. Recent data have shown the presence of four glycoproteins (ZP1, ZP2, ZP3, and ZP4) in the ZP of human and rat rather than the three glycoproteins proposed in the mouse model. In the hamster (Mesocricetus auratus), it was previously described that ZP was composed of three different glycoproteins, called ZP1, ZP2, and ZP3, even though only ZP2 and ZP3 have been cloned thus far. The aim of the study was to determine whether hamster might also express four, rather than three, ZP proteins. The full-length cDNAs encoding hamster ZP glycoproteins 1 and 4 were isolated using rapid amplification cDNA ends (RACE). The cDNA of ZP1 contains an open reading frame of 1851 nucleotides encoding a polypeptide of 616 amino acid residues. The amino acid sequence of ZP1 revealed a high homology with other mammalian species like human (66%), rat (80%), and mouse (80%). The cDNA of ZP4 contains an open reading frame of 1632 nucleotides encoding a polypeptide of 543 amino acid residues. The deduced amino acid sequence of ZP4 revealed high overall homology with rat (82%) and human (78%). Subsequent mass spectrometric analysis of the hamster ZP allowed identification of peptides from all four glycoproteins. The data presented in this study provide evidence, for the first time, that the hamster ZP matrix is composed of four glycoproteins.
authors.affiliation: Department of Cell Biology, School of Medicine, University of Murcia, 30100 Murcia, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Izquierdo-Rico
authors.forename: M
authors.initials: M
authors.lastname: Jimenez-Movilla
authors.forename: E
authors.initials: E
authors.lastname: Llop
authors.forename: A B
authors.initials: AB
authors.lastname: Perez-Oliva
authors.forename: J
authors.initials: J
authors.lastname: Ballesta
authors.forename: R
authors.initials: R
authors.lastname: Gutierrez-Gallego
authors.forename: C
authors.initials: C
authors.lastname: Jimenez-Cervantes
authors.forename: M
authors.initials: M
authors.lastname: Aviles
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D004527:Egg Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008647:Mesocricetus; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment; D015044:Zona Pellucida / Q000737:chemistry*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; C000606514:ZP1 protein, human; C000606515:ZP2 protein, human; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins; C000606519:Zp1 protein, mouse; C000606520:Zp2 protein, mouse; C000606521:Zp3 protein, mouse; C000606522:Zp4 protein, rat