title: Effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats using microarray technology.
abstract: OBJECTIVE
  We have used microarray technology to detect the effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats.
  
  
  METHODS
  Male Sprague-Dawley rats (180-200 g) were randomly divided into three groups: sham-operated; coronary artery ligation; and coronary artery ligation plus administration of Guanxin No.2 decoction (10.0 g raw materials/kg per day by gavage). The experiment was carried out on day seven after ligation.
  
  
  RESULTS
  We found that the gene expression using microarray technology showed many differences in the border infarcted left ventricular area compared with the remote noninfarcted left ventricular area after administration of Guanxin No.2 decoction.
  
  
  CONCLUSIONS
  Guanxin No.2 decoction has a long history in treating ischaemic cardiomyopathy in China, but the molecular mechanism has been unclear. In this study we found that some important genes may have contributed to the cardioprotective effect of Guanxin No.2 decoction.
authors.affiliation: James D Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, Zhejiang, P. R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zeng
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Xianzhe
authors.initials: X
authors.lastname: Yang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Hong
authors.forename: Limao
authors.initials: L
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification; D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification; D002316:Cardiotonic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D031187:Carthamus tinctorius / Q000737:chemistry; D002396:Catechols / Q000737:chemistry / Q000302:isolation & purification; D002599:Chalcone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification; D035782:Dalbergia / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification; D007773:Lactates / Q000737:chemistry / Q000302:isolation & purification; D030004:Ligusticum; D008297:Male; D039821:Monoterpenes; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D029596:Paeonia / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027561:Salvia miltiorrhiza / Q000737:chemistry; D006027:Glycosides; D053630:Depsides
chemical_list: D001547:Benzaldehydes; D001565:Benzoates; D001572:Benzofurans; D001952:Bridged-Ring Compounds; D002316:Cardiotonic Agents; D002396:Catechols; D002599:Chalcone; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D062385:Hydroxybenzoates; D007773:Lactates; D039821:Monoterpenes; D010936:Plant Extracts; D011809:Quinones; C539574:guanxin No. 2; C085278:hydroxysafflor yellow A; C015423:peoniflorin; D006027:Glycosides; C009091:protocatechuic acid; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; C004999:ferulic acid; C076944:salvianolic acid B; D053630:Depsides; C101574:chuangxiong extract