title: [Identification of Gynostemma from their adulterant Cayratia japonica by PCR-RFLP].
abstract: OBJECTIVE
  To establish a convenient and effective method for the identification of Gynostemma and Cayratia japonica.
  
  
  METHODS
  Eight species, including Gynostemm pentaphyllum, G. pentagynum, G. cardiospermum, G. longipe, G. yixingense, G. laxiflorum, G. guangxiense and C. japonica were investigated through PCR - RFLP of six chloroplast DNA fragments. The six gene fragments were digested by six restriction endonuclease respectively, including Taq I, Hpa II, EcoR I, Rsa I, Hha I, Hind III.
  
  
  RESULTS
  Seven species of Gynostemma and their adulterant could be identified by trnK1f-trnK2r and Rsa.
  
  
  CONCLUSIONS
  PCR - RFLP provides a quick, reliable molecular marker technique for identification of Cynostemma and their adulterant Cayratia japonica.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Jiang
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Li
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Zhao
mesh_terms: D018742:DNA, Chloroplast / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D029774:Gynostemma / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D027844:Vitaceae / Q000145:classification* / Q000235:genetics*
chemical_list: D018742:DNA, Chloroplast; D018744:DNA, Plant