An RNA hairpin sequesters the ribosome binding site of the homing endonuclease mobE gene.
Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced beta-galactosidase activity, consistent with translational regulation.
Gibb
Ewan A
EA
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Canada.
Edgell
David R
DR
D016980:Aeromonas hydrophila / Q000235:genetics / Q000378:metabolism / Q000821:virology; D001435:Bacteriophages / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D020029:Base Pairing; D001483:Base Sequence; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D011485:Protein Binding; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D012367:RNA, Viral; D014764:Viral Proteins; D004720:Endonucleases