title: Differential CpG island methylation of murine adenine nucleotide translocase genes.
abstract: Adenine nucleotide translocase (Ant) mediates the exchange of ADP and ATP across the inner mitochondrial membrane in eukaryotes. Mice possess three distinct but highly homologous Ant isoforms, encoded by independent genes, whose transcription depends upon tissue type. Ant1 is expressed selectively in heart and skeletal muscles, Ant2 is ubiquitously expressed in most tissues but lower in skeletal muscle and testis, while Ant4 is exclusively expressed in the testis. Of interest, each of these Ant genes contains CpG islands in their proximal promoter regions. We investigated the methylation status of the three Ant genes in various tissues with active and inactive transcription. In contrast to the Ant4 gene in which CpG island methylation is essential for gene repression, the CpG islands of Ant1 and Ant2 are hypomethylated regardless of the gene expression status throughout the tissues of male mice. Despite the tissue specific expression profile of Ant1, CpG methylation is unlikely involved in the regulation of the gene. Consistent with these findings, addition of a CpG-demethylating agent, 5-aza-2'-deoxycitidine, to fibroblasts increased the expression of Ant4 but not Ant1 or Ant2 genes. This study provides insight regarding the differential regulation of Ant isoforms in mammals, whereby both the Ant1 and Ant2 genes are capable of expression, but the Ant4 gene is completely repressed throughout somatic tissues. To the best of our knowledge, this is a first example to clearly demonstrate a differential usage of CpG island methylation within a family of genes.
authors.affiliation: Department of Pathology, University of Florida College of Medicine, P. O. Box 100275, Gainesville, FL 32610, USA.
authors.forename: Jeffrey V
authors.initials: JV
authors.lastname: Brower
authors.forename: Chae Ho
authors.initials: CH
authors.lastname: Lim
authors.forename: Chul
authors.initials: C
authors.lastname: Han
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Hankowski
authors.forename: Takashi
authors.initials: T
authors.lastname: Hamazaki
authors.forename: Naohiro
authors.initials: N
authors.lastname: Terada
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D018899:CpG Islands; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors; D004262:DNA Restriction Enzymes; D000077209:Decitabine; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D005796:Genes; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics* / Q000378:metabolism; D041681:NIH 3T3 Cells; D011401:Promoter Regions, Genetic; D013447:Sulfites
chemical_list: D007527:Isoenzymes; D013447:Sulfites; D000077209:Decitabine; D004247:DNA; D000226:Mitochondrial ADP, ATP Translocases; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes; D001374:Azacitidine