title: [Effect of nobiletin on tubulin polymerization in vitro].
abstract: OBJECTIVE
  To investigate the possible mechanisms of nobiletin for anticancer by studying the inhibition effects of nobiletin on tubulin polymerization.
  
  
  METHODS
  In vitro nobiletin was added into the tubulin polymerization-depolymerization system and the absorption values were recorded at 350 nm under 37 degrees C.
  
  
  RESULTS
  As compared with controls, the absorption values in reaction system decreased significantly in nobiletin treatment groups. When nobiletin final concentrations in reaction system were 5.0, 7.5, 10.0 and 12.5 micromol x L(-1), the maximum absorption values were 0 130, 0.109, 0.086 and 0.071 with 16.7%, 30.1%, 44.9% and 54.5% of inhibition rate, respectively. The results suggested that nobiletin could inhibit tubulin polymerization.
  
  
  CONCLUSIONS
  The inhibition effect of nobiletin on tubulin polymerization is the possible mechanism for anticancer.
authors.affiliation: Guangdong Medical College, Guangdong Key Laboratory for Research and Development of Nature Drugs, Zhanjiang 524023, China. plliu78@sina.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Ke-Feng
authors.initials: KF
authors.lastname: Wu
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Li
mesh_terms: D000818:Animals; D047309:Flavones / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D013552:Swine; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism*; D050257:Tubulin Modulators / Q000494:pharmacology*
chemical_list: D047309:Flavones; D014404:Tubulin; D050257:Tubulin Modulators; C008661:nobiletin