title: Parallel screening and optimization of protein constructs for structural studies.
abstract: A major challenge in structural biology remains the identification of protein constructs amenable to structural characterization. Here, we present a simple method for parallel expression, labeling, and purification of protein constructs (up to 80 kDa) combined with rapid evaluation by NMR spectroscopy. Our approach, which is equally applicable for manual or automated implementation, offers an efficient way to identify and optimize protein constructs for NMR or X-ray crystallographic investigations.
authors.affiliation: Institut de Biologie Structurale Jean-Pierre Ebel, Centre National de la Recherche Scientifique, Université Joseph-Fourier, Grenoble, France.
authors.forename: Rodolfo M
authors.initials: RM
authors.lastname: Rasia
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Noirclerc-Savoye
authors.forename: Nicolás G
authors.initials: NG
authors.lastname: Bologna
authors.forename: Benoit
authors.initials: B
authors.lastname: Gallet
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Plevin
authors.forename: Laurence
authors.initials: L
authors.lastname: Blanchard
authors.forename: Javier F
authors.initials: JF
authors.lastname: Palatnik
authors.forename: Bernhard
authors.initials: B
authors.lastname: Brutscher
authors.forename: Thierry
authors.initials: T
authors.lastname: Vernet
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boisbouvier
mesh_terms: D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D007553:Isotope Labeling; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C420966:HYL1 protein, Arabidopsis; D009587:Nitrogen Isotopes; D011506:Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins