[Expression of transcription factor LYL1 in leukemia and its possible role in leukemogenesis].
OBJECTIVE
To study the expression of transcription factor LYL1 in leukemia and its possible role in leukemogenesis.


METHODS
Fluorescence real time quantitative polymerase chain reaction was used to detect the expression levels of LYL1 in leukemias. Specific siRNA was used to silence the expression of LYL1 in K562 cells.


RESULTS
Compared to CD34 positive cells from normal bone marrow, the expression of LYL1 was significantly elevated in patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). LYL1 expression was higher in chronic myeloid leukemia (CML) in blastic crisis than that in chronic phase (7.831 vs 1.672, P < 0.01). LYL1 expression in AML in complete remission (CR) was down-regulated as compared with that of un-remission patients (1.400 vs 9.985, P < 0.01). Down-regulation of endogenous expression of LYL1 in K562 cells by a combination of three specific siRNA could inhibit cellular growth and clonogenicity to some extent.


CONCLUSIONS
Over-expression of LYL1 is highly associated with AML as well as ALL. RNA interference targeting specific oncogenes such as LYL1 is potentially useful in the treatment of hematological malignancies.
Meng
Yue-sheng
YS
Department of Hematology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
Zhang
Yan-xiang
YX
Ai
Gong-wen
GW
Meng
Xiu-qin
XQ
Liu
Wei
W
Wei
Rong
R
Jiang
Wei
W
D000328:Adult; D000368:Aged; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D055815:Young Adult
D051792:Basic Helix-Loop-Helix Proteins; D009363:Neoplasm Proteins; C086541:LYL1 protein, human