Roles of glia limitans astrocytes and carbon monoxide in adenosine diphosphate-induced pial arteriolar dilation in newborn pigs.
OBJECTIVE
Astrocytes, neurons, and microvessels together form a neurovascular unit allowing blood flow to match neuronal activity. Adenosine diphosphate (ADP) is an important signaling molecule in the brain, and dilation in response to ADP is astrocyte-dependent in rats and newborn pigs. Carbon monoxide (CO), produced endogenously by catabolism of heme to CO, iron, and biliverdin via heme oxygenase, is an important cell-signaling molecule in the neonatal cerebral circulation. We hypothesize ADP stimulates CO production by glia limitans astrocytes and that this CO causes pial arteriolar dilation.


METHODS
Experiments were performed using anesthetized piglet with closed cranial windows, and freshly isolated piglet astrocytes and microvessels. Astrocyte injury was caused by topical application of L-2-alpha aminoadipic acid (2 mmol/L, 5 hours). Cerebrospinal fluid was collected from under the cranial windows for measurement of ADP-stimulated CO production. CO was measured by gas chromatography-mass spectroscopy analysis.


RESULTS
Before, but not after, astrocyte injury in vivo, topical ADP stimulated both CO production and dilation of pial arterioles. Astrocyte injury did not block dilation to isoproterenol or bradykinin. Chromium mesoporphyrin, an inhibitor of heme oxygenase, also prevented the ADP-induced increase in cerebrospinal fluid CO and pial arteriolar dilation caused by ADP, but not dilation to sodium nitroprusside. ADP also increased CO production by freshly isolated piglet astrocytes and cerebral microvessels, although the increase was smaller in the microvessels.


CONCLUSIONS
These data suggest that glia limitans astrocytes use CO as a gasotransmitter to cause pial arteriolar dilation in response to ADP.
Kanu
Alie
A
Laboratory for Research in Neonatal Physiology, Department of Physiology, University of Tennessee Health Science Center, Memphis, Tenn., USA.
Leffler
Charles W
CW
D015074:2-Aminoadipic Acid / Q000633:toxicity; D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001253:Astrocytes / Q000502:physiology*; D002196:Capillaries / Q000378:metabolism; D002248:Carbon Monoxide / Q000502:physiology*; D002536:Cerebral Arteries / Q000187:drug effects*; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D009457:Neuroglia / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D013552:Swine; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology*; D014665:Vasodilator Agents
D000318:Adrenergic beta-Agonists; D018691:Excitatory Amino Acid Antagonists; D014665:Vasodilator Agents; D009599:Nitroprusside; D015074:2-Aminoadipic Acid; D000244:Adenosine Diphosphate; D002248:Carbon Monoxide; D007545:Isoproterenol