Ferrocenyl-doped silica nanoparticles as an immobilized affinity support for electrochemical immunoassay of cancer antigen 15-3.
The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of Fc-COOH and were easily modified with trialkoxysilane reagents for covalent conjugation of CA 15-3 antibodies (anti-CA 15-3). The Fc-COOH-doped SNPs were characterized by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The fabrication process of the electrochemical immunosensor was demonstrated by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimal conditions, the developed immunosensor showed good linearity at the studied concentration range of 2.0-240 UmL(-1) with a coefficient 0.9986 and a detection limit of 0.64 UmL(-1) at S/N=3.
Hong
Chenglin
C
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Yuan
Ruo
R
Chai
Yaqin
Y
Zhuo
Ying
Y
D000941:Antigens / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002138:Calibration; D055664:Electrochemical Techniques; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D000075163:Metallocenes; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013997:Time Factors
D000941:Antigens; D014408:Biomarkers, Tumor; D005296:Ferrous Compounds; D000075163:Metallocenes; C032949:ferrocenecarboxylic acid; D012822:Silicon Dioxide