title: Production of oligosaccharides and cellobionic acid by Fibrobacter succinogenes S85 growing on sugars, cellulose and wheat straw.
abstract: Extracellular culture fluid of Fibrobacter succinogenes S85 grown on glucose, cellobiose, cellulose or wheat straw was analysed by 2D-NMR spectroscopy. Cellodextrins did not accumulate in the culture medium of cells grown on cellulose or straw. Maltodextrins and maltodextrin-1P were identified in the culture medium of glucose, cellobiose and cellulose grown cells. New glucose derivatives were identified in the culture fluid under all the substrate conditions. In particular, a compound identified as cellobionic acid accumulated at high levels in the medium of F. succinogenes S85 cultures. The production of cellobionic acid (and cellobionolactone also identified) was very surprising in an anaerobic bacterium. The results suggest metabolic shifts when cells were growing on solid substrate cellulose or straw compared to soluble sugars.
authors.affiliation: Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 Université Blaise Pascal-CNRS, 63177 Aubière Cdex, France.
authors.forename: Régis
authors.initials: R
authors.lastname: Nouaille
authors.forename: Maria
authors.initials: M
authors.lastname: Matulova
authors.forename: Vladimír
authors.initials: V
authors.lastname: Pätoprstý
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Delort
authors.forename: Evelyne
authors.initials: E
authors.lastname: Forano
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism*; D045848:Fibrobacter / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry
chemical_list: D003470:Culture Media; D004187:Disaccharides; D009844:Oligosaccharides; C006938:cellobionic acid; D002482:Cellulose