title: Accuracy and prognostic impact of a vessel invasion grading system for stage IA non-small cell lung cancer.
abstract: BACKGROUND
  Despite the recognition that lymphatic and blood vessel invasion is an important prognostic factor in lung cancer, there is no common definition for pathological evaluation of vessel invasion. The aim of the present study was to determine whether D2-40 immunostaining can increase the accuracy of detection of lymphatic vessel invasion and whether our new grading system of vessel invasion by "degree" could be used instead of conventional evaluation by "presence" for pathological stage IA non-small cell lung cancer (NSCLC).
  
  
  METHODS
  The vessel invasion classification was re-evaluated in 221 recent paraffin-embedded sections of p-stage IA NSCLC stained by Hematoxylin-Eosin (HE), Elastica-Van-Gieson (EVG), and D2-40.
  
  
  RESULTS
  After re-assessment using D2-40 immunostaining, 41.2% (31 of 75) of ly1 cases by HE/EVG changed to ly0, and 14.9% (17 of 114) of ly0 cases by HE/EVG changed to ly1. Overall, 4 of 28 ly2 cases on conventional staining were changed to ly1, and 2 were changed to ly0 using D2-40 immunostaining. When the patients were divided into two groups by the presence of vessel invasion (v/ly0 vs. 1, 2, 3), there was no significant difference in cancer-specific survival (p=0.1107, 0.0875, respectively), while when they were divided according to degree of vessel invasion (v/ly0, 1 vs. 2, 3), there was a statistically significant difference (p=0.0038, p=0.0002, respectively). On multivariate analysis, lymphatic vessel invasion had a significant impact on cancer-specific survival (p=0.0061).
  
  
  CONCLUSIONS
  Our results suggest that D2-40 immunostaining provides a precise diagnosis of lymphatic vessel invasion, and our new grading system of vessel invasion by "degree" is accurate and has prognostic value in early lung cancer.
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hashizume
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagayasu
authors.forename: Tomayoshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Hidaka
authors.forename: Tomoshi
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tagawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Yamasaki
authors.forename: Katsuro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Keitaro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Takuro
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D014408:Biomarkers, Tumor / Q000276:immunology* / Q000378:metabolism; D001808:Blood Vessels / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology / Q000503:physiopathology; D055088:Early Detection of Cancer; D004729:Endothelium, Lymphatic / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology / Q000503:physiopathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012795:Sialoglycoproteins / Q000276:immunology* / Q000378:metabolism; D016019:Survival Analysis; D047368:Tumor Burden
chemical_list: D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D014408:Biomarkers, Tumor; D012795:Sialoglycoproteins; C500987:monoclonal antibody D2-40