title: Fibronectin promotes the phorbol 12-myristate 13-acetate-induced macrophage differentiation in myeloid leukemia cells.
abstract: The extracellular matrix plays a critical role in macrophage maturation. In this study, the HL60 cell line was used as a model of leukemic myeloid cell differentiation. We assessed the ability of HL60 cells cultured on fibronectin substratum prior to phorbol 12-myristate 13-acetate (PMA) induction to differentiate into terminally differentiated macrophages. Beside their distinctive macrophage morphology, they expressed antigen receptors CD14, TLR2, TLR4 and CD68, and displayed enhanced phagocytic activity and production of reactive oxygen species. Expression of CD13, CD33, CD15 and alpha-naphthyl-acetate esterase was also maintained, however, differentiated HL60 cells were HLA-DR and CD1a negative. Here, we describe the enhanced capacity of fibronectin-adherent HL60 cells to differentiate into macrophages in response to PMA.
authors.affiliation: Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey. gunes.esendagli@lycos.com.
authors.forename: Gunes
authors.initials: G
authors.lastname: Esendagli
authors.affiliation: Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey.
authors.forename: Hande
authors.initials: H
authors.lastname: Canpinar
authors.affiliation: Department of Pathology, Faculty of Medicine, Ankara University, Ankara, Turkey.|Department of Pathology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors.forename: Guldal
authors.initials: G
authors.lastname: Yilmaz
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: F Figen
authors.initials: FF
authors.lastname: Kaymaz
authors.affiliation: Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Kansu
authors.affiliation: Department of Basic Oncology, Institute of Oncology, Hacettepe University, Sihhiye, 06100, Ankara, Turkey.
authors.forename: Dicle
authors.initials: D
authors.lastname: Guc
mesh_terms: D005353:Fibronectins / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D008264:Macrophages / Q000166:cytology; D022423:Myeloid Cells / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D005353:Fibronectins; D013755:Tetradecanoylphorbol Acetate