title: Do external stimuli, applied to train cultured cortical networks, disturb the balance between activity and connectivity?
abstract: Learning, or more generally, plasticity may be studied using cultured neuronal networks on multi electrode arrays. Many protocols have been proposed to change connectivity in such networks. So far, only one of these protocols, proposed by Shahaf and Marom, aimed to change the input-output relationship of a selected connection in the network. Although the results were quite promising, the experiments appeared difficult to repeat and the protocol did not serve as a basis for wider investigation yet. Here, we repeated their protocol, and compared our 'learning curves' to the original results. Although in some experiments the protocol did not seem to work, we found that on average, the protocol showed a significant learning effect indeed. We frequently found learning curves that initially declined as in the original results, but then increased again before finally settling at a low level.
authors.affiliation: Biomedical Technology, Department of Electrical Engineering, Mathematics and Computer science, Biomedical Signals and Systems Group, University of Twente, PO Box 217, 7500 AE, Enschede, The Netherlands. j.lefeber@utwente.nl
authors.forename: Joost
authors.initials: J
authors.lastname: le Feber
authors.forename: Jan
authors.initials: J
authors.lastname: Stegenga
authors.forename: Wim
authors.initials: W
authors.lastname: Rutten
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology*; D003198:Computer Simulation; D004558:Electric Stimulation / Q000379:methods*; D007858:Learning / Q000502:physiology*; D008959:Models, Neurological; D009415:Nerve Net / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000502:physiology*