Expression analysis of phytohormone-regulated microRNAs in rice, implying their regulation roles in plant hormone signaling.
Twenty-two conserved miRNAs were chosen to investigate the expression pattern in response to phytohormone treatments, in which the effects of five classic plant hormone stresses were surveyed in Oryza sativa. The results showed that 11 miRNAs were found to be dysregulated by one or more phytohormone treatments. The target genes of these miRNAs were validated in vivo and their expression profiling were revealed. We also analyzed the promoter regions of the 22 conserved miRNAs for phytohormone-responsive elements and the existence of the elements provided further evidences supporting our results. These findings enable us to further investigate the role of miRNAs in phytohormone signaling.
Liu
Qing
Q
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Biotechnology Research Center, Sun Yat-sen University, Guangzhou 510275, PR China.
Zhang
Yu-Chan
YC
Wang
Cong-Ying
CY
Luo
Yu-Chun
YC
Huang
Qiao-Juan
QJ
Chen
Shao-Yu
SY
Zhou
Hui
H
Qu
Liang-Hu
LH
Chen
Yue-Qin
YQ
D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D015398:Signal Transduction / Q000235:genetics*
D035683:MicroRNAs; D010937:Plant Growth Regulators; D018749:RNA, Plant