A cooperative polymer-DNA microarray approach to biomaterial investigation.
In this study, polymer microarrays were used for the rapid identification of polymer substrates upon which a suspension cell line would both adhere and proliferate giving a detailed and rapid understanding of cell-biomaterial interactions. Analysis demonstrated that suspension K562 human erythroleukemic cells, which normally grow in suspension, adhered and proliferated on several different polymers. Phenotypic and transcriptomic analysis techniques allowed examination of the interaction between cells and polymers permitting the elucidation of putative links between phenotypic responses to cell-biomaterial interactions and global gene expression.
Pernagallo
Salvatore
S
School of Chemistry, University of Edinburgh, EH9 3JJ, Edinburgh, UK.
Diaz-Mochon
Juan Jose
JJ
Bradley
Mark
M
D000180:Acrylic Resins / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics; D049109:Cell Proliferation; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D020014:K562 Cells; D046228:Microarray Analysis / Q000379:methods*; D033401:Microscopy, Acoustic; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011108:Polymers / Q000378:metabolism*; D011140:Polyurethanes / Q000378:metabolism; D015203:Reproducibility of Results
D000180:Acrylic Resins; D001672:Biocompatible Materials; D011108:Polymers; D011140:Polyurethanes