Identification of an optimal concentration of platelet gel for promoting angiogenesis in human endothelial cells.
BACKGROUND
Numerous studies have supported the use of topical blood components to improve wound healing and tissue regeneration. Platelet gel (PG), a hemocomponent obtained from mix of activated platelets (PLTs) and cryoprecipitate, is currently being used clinically in an attempt to improve tissue healing. The present study sought to define the most effective PG concentration to promote angiogenesis in vitro.


METHODS
The effects of PG-released supernatant at different concentrations on human endothelial cells were studied using different in vitro assays (proliferation, migration, invasion, cord formation, and wound healing).


RESULTS
The concentration of PG-released supernatant had a significant influence on the angiogenic potential of endothelial cells. The optimal concentration for the stimulation of angiogenesis was 1.5 x 10(6) PLTs per microL in most of the in vitro experiments used in this study. Lower or higher concentrations of PG displayed a lower angiogenic potential.


CONCLUSIONS
An optimal concentration of PG to promote angiogenesis in human endothelial cells was identified. Excessively high PG concentrations may inhibit the angiogenic process, thereby being counterproductive for wound healing in a clinical setting.
Giusti
Ilaria
I
Department of Health Sciences, University of L'Aquila, L'Aquila, Italy.
Rughetti
Anna
A
D'Ascenzo
Sandra
S
Millimaggi
Danilo
D
Pavan
Antonio
A
Dell'Orso
Luigi
L
Dolo
Vincenza
V
D000818:Animals; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005782:Gels / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009994:Osmolar Concentration
D017077:Culture Media, Conditioned; D005782:Gels