title: Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLCgamma and cooperate to modulate embryonic stem cell survival and proliferation.
abstract: Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.
authors.affiliation: Institute of Molecular and Cellular Medicine, Kunming Medical College, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Li
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Zeng
authors.forename: Gavin S
authors.initials: GS
authors.lastname: Dawe
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Gabriele
authors.initials: G
authors.lastname: Loers
authors.forename: Thomas
authors.initials: T
authors.lastname: Tilling
authors.forename: Shu-Sen
authors.initials: SS
authors.lastname: Cui
authors.forename: Melitta
authors.initials: M
authors.lastname: Schachner
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D005562:Formazans / Q000378:metabolism; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006031:Glycosylation; D051379:Mice; D041681:NIH 3T3 Cells; D010740:Phospholipases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000378:metabolism; D014162:Transfection
chemical_list: D005562:Formazans; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; C079782:MTT formazan; D005647:Fucosyltransferases; D012799:Sialyltransferases; C029156:N-acetyllactosaminide alpha-2,3-sialyltransferase; D010740:Phospholipases; D001973:Bromodeoxyuridine