Serum and tissue expression of transforming growth factor beta 1 in  psoriasis.
BACKGROUND
In psoriasis, keratinocyte hyperplasia may be explained by imbalance of growth factors responsible for epidermal proliferation and altered metabolism of their receptors. Transforming growth factor-beta 1 (TGF-beta1) implications in the pathogenesis of psoriasis can be attributed to several mechanisms besides keratinocyte cell cycle inhibition.


OBJECTIVE
To evaluate the relation between serum and tissue levels of TGF-beta1 in psoriasis and their correlation with disease parameters.


METHODS
Serum and punch biopsy of involved and non-involved skin of 22 patients with psoriasis vulgaris and 10 controls were collected for quantification of TGF-beta1 by enzyme-linked immunosorbent assay kit.


RESULTS
Serum level of TGF-beta1 in psoriatic patients was higher than controls in a statistically non-significant manner. Correlations between serum level of TGF-beta1 and extent of the disease (P = 0.007) and Psoriasis Area and Severity Index (PASI) score (P = 0.005) were observed. Mean tissue levels of TGF-beta1 were highest in psoriatic lesions in contrast to normal skin of psoriatic patients and healthy controls, but not statistically significant. Correlation between tissue levels of TGF-beta1 in non-involved skin and extent of the disease (P = 0.007) and PASI score (P = 0.013) was detected. Correlation was detected between levels of TGF-beta1 in psoriatic plaques and serum of patients (P = 0.035), but not between levels of TGF-beta1 in non-involved skin and serum.


CONCLUSIONS
Tissue expression of TGF-beta1 in psoriasis may be affected by the stage of development of the lesion. The direct relation between TGF-beta1 in psoriatic plaques and serum imply that the mechanisms for TGF-beta1 production and release in both these compartments may be related.
Zaher
H
H
Department of Dermatology, Cairo University, Cairo, Egypt.
Shaker
O G
OG
EL-Komy
M H M
MH
El-Tawdi
A
A
Fawzi
M
M
Kadry
D
D
D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000097:blood / Q000378:metabolism*
C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1