Zn finger-based direct detection system for PCR products of Salmonella spp. and the Influenza A virus.
We have detected PCR products from Salmonella spp. and Influenza A virus using Zn finger protein Zif268 and Sp1, respectively. Previously, we demonstrated a novel method of rapid and specific detection of PCR products from Legionella pneumophila genome using Zn finger protein Sp1. In principle, this methodology might be applied to the detection of most bacteria and viruses using various Zn finger proteins. Here, to demonstrate the wider applicability of our method, we detected PCR products from Salmonella spp. and the Influenza A virus. BLAST data indicated the Zif268 and Sp1 recognition sequence were located on the gyrB gene of Salmonella spp. and the nucleoprotein gene of Influenza A virus, respectively. The PCR products from the oligonucleotide corresponding to the gyrB gene of Salmonella spp. or the nucleoprotein gene of the Influenza A virus could be specifically detected by ELISA or fluorescence depolarization measurement using Zif268 or Sp1. These results indicate the wide applicability of our novel methodology.
Osawa
Yuko
Y
Department of Biotechnology and Life Science, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Ikebukuro
Kazunori
K
Kumagai
Takenori
T
Motoki
Hiroaki
H
Matsuo
Takafumi
T
Horiuchi
Michio
M
Sode
Koji
K
D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D009980:Influenza A virus / Q000302:isolation & purification*; D019590:Nucleocapsid Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D016601:RNA-Binding Proteins / Q000235:genetics; D012475:Salmonella / Q000302:isolation & purification*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014758:Viral Core Proteins / Q000235:genetics
D004269:DNA, Bacterial; D004279:DNA, Viral; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D016329:Sp1 Transcription Factor; D014758:Viral Core Proteins; D027081:DNA Gyrase