Contrast enhancement of speckle patterns from blood in synchrotron X-ray imaging.
Hemodynamics has been a very important factor in understanding and diagnosing various vascular diseases. Recently, the X-ray particle image velocimetry (X-ray PIV) method using speckle patterns of blood has been introduced as a new quantitative visualization method for blood flows without any seeding tracer or contrast agents. In this study, the peculiar optical characteristics of blood on the synchrotron X-ray imaging method, which were not presented in previous studies, were investigated in depth and systematically. The experimental conditions required for X-ray PIV application were found to be the distance between the sample and the scintillator ( approximately 40cm), the thickening of the blood sample (>0.3mm), and hematocrit (20.0-80.0%). In addition, we verified that the X-ray PIV method is reliable as an advanced flow velocimetry by comparing the flow rate evaluated from the X-ray PIV result and the input flow rate supplied from a syringe pump with an error of less than 1%. Through this study, based on the understanding of contrast enhancement mechanisms of speckle patterns from blood, we could establish a trustworthy flow visualization method that can be used effectively in hemodynamic studies.
Kim
Guk Bae
GB
Center for Neural Science, Korea Institute of Science and Technology, Republic of Korea.
Lee
Sang Joon
SJ
D001775:Blood Circulation / Q000502:physiology*; D003287:Contrast Media / Q000032:analysis*; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods*; D006400:Hematocrit; D017356:Synchrotrons; D014965:X-Rays
D003287:Contrast Media