title: [Studies on separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins].
abstract: OBJECTIVE
  To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins.
  
  
  METHODS
  Based on absorptive capacity and elution efficiency of total saponin from flower buds of Panax notoginseng, the best type of resin and the conditions to separate and purify total saponin by macroporous resins were optimized.
  
  
  RESULTS
  The AB-8 resin was chosen, washed with 3 BV of 70% ethanol, and with the absorption and elution velocity of flow 2BV/h. The optimal concentration of the raw material was 0. 2 g/ml. The purity of total saponin obtained was 87.60%.
  
  
  CONCLUSIONS
  Using AB-8 resin to separate and purify total saponin from flowers of Panax notoginseng is feasible.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. weilitcm@sina.com
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000042:Absorption; D000431:Ethanol / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D053723:Panax notoginseng / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D012117:Resins, Synthetic; D012503:Saponins; D012997:Solvents; D000431:Ethanol