title: [Molecular subtyping of Staphylococcus aureus isolated from a severe food-poisoning].
abstract: OBJECTIVE
  To study the molecular types of Staphylococcus aureus isolated from a severe food-poisoning and to trace the possible strains.
  
  
  METHODS
  Real-time PCR was applied to detect nuc gene as a specific marker for S. aureus, mecA gene encoding methicillin resistance and 5 other genes encoding staphylococcal enterotoxins (sea, seb, see, sed, see). Isolates were also performed with 16S rRNA oligonucleotide sequence analyzing by DNAStar MegAlign 5.0 software and pulse-field gel electrophoresis (PFGE) by BioNumerics Version 4.0 software.
  
  
  RESULTS
  The nuc gene was detected from the 10 isolated strains, sea and seb genes were detected from 7 strains. There were 4 16 S rRNA types and 5 PFGE types found from all the strains.
  
  
  CONCLUSIONS
  Three relative S. aureus strains were involved in the severe food-poisoning at least. Molecular subtyping might give a molecular epidemiological evidence and support the source tracing of an outbreak.
authors.affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zi-Yao
authors.initials: ZY
authors.lastname: Mo
authors.forename: Xing-Lin
authors.initials: XL
authors.lastname: Pang
authors.forename: Zhi-Ai
authors.initials: ZA
authors.lastname: Deng
authors.forename: Xin-Qiang
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Shou-Yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins; D006801:Humans; D013202:Staphylococcal Food Poisoning / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004768:Enterotoxins