Redifferentiation of human hepatoma cells (SMMC-7721) induced by two new highly oxygenated bisabolane-type sesquiterpenes.
Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour,antifungal, antibacterial,antioxidant and antivenom properties.We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS)isolated from Cremanthodium discoideum (C.discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells.Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover,HOBS was able to increase tyrosine-alpha ketoglutarate transaminase activity,decrease alpha- foetoprotein level and gamma-glutamyl transferase activity. In addition,we found that HOBS inhibited the anchorage- independent growth of SMMC-7721 cells in a dose-dependent manner.Taken together,all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.
Miao
Ruidong
R
School of Life Sciences,Lanzhou University, Lanzhou 730000, P R China.
Wei
Juan
J
Zhang
Qi
Q
Sajja
Venkateswara
V
Yang
Jinbo
J
Wang
Qin
Q
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000145:classification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000737:chemistry / Q000145:classification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D000509:alpha-Fetoproteins / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
C539457:1beta,8-diangeloyloxy-2beta-acetoxy-4alpha-chloro-11-methoxy-3beta, 10-dihydroxybisabola-7(14)-ene; C539458:1beta,8-diangeloyloxy-2beta-acetoxy-4alpha-chloro-3beta-hydroxy-10, 11-o,o-isopropylidenebis-aboia-7(14)-ene; D000972:Antineoplastic Agents, Phytogenic; D012717:Sesquiterpenes; D000509:alpha-Fetoproteins; D005723:gamma-Glutamyltransferase