[The changes of AMPA receptors subunit GluR2 and mEPSCs in visual cortex of monocular deprivation rats].
OBJECTIVE
To observe the change of expression in quantity of AMPA receptor subunit GluR2 and mEPSCs from monocular derivated rats. The preparative work was conducted for the further study on mechanism involved in regulation and control of the visual development plasticity.


METHODS
It was an experimental study. Forty 14 d healthy Wistar rats were divided into two groups randomly; twenty in each group which were divided into two groups (I, II) and derivated monocular in experiment group (group I), and then were killed at a week later. ForIA and IIA 5 microm thick section was cut from tissue of visual cortex in coronary side, then the expression of GluR2 was observed by microscope and image analyze of GluR2 was conducted after immunohistochemical staining; for IB and IIB, mEPSCs was investigated which was used Voltage-clamp technique with whole-cell mode.


RESULTS
The level of GluR2 in visual cortex of normal rats was higher than experiment group. The mean amplitude and frequency of mEPSCs increased in the derivated monocular group.


CONCLUSIONS
The level of GluR2 has changed association with the visual stimulation, which suggests that GluR2 should have the important physiological function on the postnatal development of visual system of rats.
Hao
Rui
R
Tianjin Medical University, Tianjin Eye Hospital, Tianjin 300020, China.
Xing
Yong-Xin
YX
Zhao
Kan-Xing
KX
D000818:Animals; D019706:Excitatory Postsynaptic Potentials; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism*; D009435:Synaptic Transmission; D014786:Vision Disorders / Q000378:metabolism* / Q000503:physiopathology*; D015349:Vision, Monocular; D014793:Visual Cortex / Q000378:metabolism / Q000502:physiology
D018091:Receptors, AMPA; C104722:glutamate receptor ionotropic, AMPA 2