title: Cryptochrome-1 expression: a new prognostic marker in B-cell chronic lymphocytic leukemia.
abstract: Chronic lymphocytic leukemia is an adult-onset leukemia with a heterogeneous clinical behavior. When chronic lymphocytic leukemia cases were divided on the basis of IgV(H) mutational status, widely differing clinical courses were revealed. Since IgV(H) sequencing is difficult to perform in a routine diagnostic laboratory, finding a surrogate for IgV(H) mutational status seems an important priority. In the present study, we proposed the use of Cryptochrome-1 as a new prognostic marker in early-stage chronic lymphocytic leukemia. Seventy patients (Binet stage A, without treatment) were included in the study. We correlated Cryptochrome-1 mRNA with well established prognostic markers such as IgV(H) mutations, ZAP70, LPL or CD38 expression and chromosomal abnormalities. High Cryptochrome-1 expression correlated with IgV(H) unmutated samples. In addition, Cryptochrome-1 was a valuable predictor of disease progression in early-stage chronic lymphocytic leukemia, therefore it can be introduced in clinical practice with the advantage of a simplified method of quantification.
authors.affiliation: Molecular Haematology Laboratory, Prince Felipe Research Centre, Valencia, Spain. ejantus@cipf.es
authors.forename: Eloisa Jantus
authors.initials: EJ
authors.lastname: Lewintre
authors.forename: Cristina Reinoso
authors.initials: CR
authors.lastname: Martín
authors.forename: Carlos García
authors.initials: CG
authors.lastname: Ballesteros
authors.forename: David
authors.initials: D
authors.lastname: Montaner
authors.forename: Rosa Farrás
authors.initials: RF
authors.lastname: Rivera
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Mayans
authors.forename: Javier
authors.initials: J
authors.lastname: García-Conde
mesh_terms: D015415:Biomarkers; D056931:Cryptochromes; D005420:Flavoproteins / Q000235:genetics*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D015415:Biomarkers; D056931:Cryptochromes; D005420:Flavoproteins; D012333:RNA, Messenger