Enhanced bone formation by marrow-derived endothelial and osteogenic cell transplantation.
Bone marrow-derived osteogenic cells can regenerate bone tissuesin vivo. The aim of the present study is to determine whether the cotransplantation of bone marrow-derived endothelial-like cells (BMECs) enhances bone regeneration by bone marrow-derived osteogenic cell (BMOC) transplantation in osseous defects. Canine bone marrow cells were differentiated separately into BMECs and BMOCs. Using apatite-coated poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds as cell delivery vehicles, BMOCs were transplanted with or without BMECs into critical-sized calvarial defects in immunodeficient mice. Histological analyses, microcomputed tomography, and soft X-ray were performed to assess mineralized bone formation at 8 weeks. Cotransplantation of BMECs and BMOCs resulted in greater bone formation than transplantation of BMOCs alone. There was a significant (p < 0.05) increase in bone formation area following cotransplantation (30.8% +/- 2.5%), compared with transplantation of BMOCs alone (15.3% +/- 1.9%). These results demonstrate that the cotransplantation of BMECs enhances bone regeneration mediated by BMOC transplantation in osseous defects.
Kim
Sang-Soo
SS
Department of Bioengineering, Hanyang University, Seoul 133-791, Korea.
Park
Min Sun
MS
Cho
Seung-Woo
SW
Kang
Sun Woong
SW
Ahn
Kang-Min
KM
Lee
Jong-Ho
JH
Kim
Byung-Soo
BS
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000000981:diagnostic imaging; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology / Q000000981:diagnostic imaging / Q000637:transplantation*; D006801:Humans; D007150:Immunohistochemistry; D010012:Osteogenesis / Q000502:physiology*; D055114:X-Ray Microtomography