cis preferential replication of Lettuce infectious yellows virus (LIYV) RNA 1: the initial step in the asynchronous replication of the LIYV genomic RNAs.
A series of Lettuce infectious yellows virus (LIYV) RNA 1 mutants was created to evaluate their ability to replicate in tobacco protoplasts. Mutants DeltaEcoRI, DeltaE-LINK, and Delta1B, having deletions in open reading frames (ORFs) 1A and 1B, did not replicate when individually inoculated to protoplasts or when co-inoculated with wild-type RNA1 as a helper virus. A fragment of the green fluorescent protein (GFP) gene was inserted into the RNA 1 ORF 2 (P34) in order to provide a unique sequence tag. This mutant, P34-GFP TAG, was capable of independent replication in protoplasts. Mutants derived from P34-GFP TAG having frameshift mutations in the ORF 1A or 1B were unable to replicate in protoplasts alone or in trans when co-inoculated with wild-type RNA1 as a helper virus. Taken together, these data strongly suggest that LIYV RNA 1 replication is cis-preferential.
Wang
Jinbo
J
Department of Plant Pathology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
Yeh
Hsin-Hung
HH
Falk
Bryce W
BW
D029383:Crinivirus / Q000235:genetics / Q000502:physiology*; D004252:DNA Mutational Analysis; D016368:Frameshift Mutation; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D011523:Protoplasts / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D017384:Sequence Deletion; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins; D049452:Green Fluorescent Proteins