Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MS(n).
Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MS(n) and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MS(n) spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.
Yue
Hao
H
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Pi
Zifeng
Z
Song
Fengrui
F
Liu
Zhiqiang
Z
Cai
Zongwei
Z
Liu
Shuying
S
D000157:Aconitine; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D000470:Alkaloids; D000157:Aconitine