title: Engineering a function into a glycosyltransferase.
abstract: As glycosyltransferases found in nature often show distinct substrate specificity, glycosyltransferase engineering is an important research field. In this work, we were able to introduce an activity into a glycosyltransferase involved in natural product (landomycin E) biosynthesis. This was achieved by recognizing hot spot amino acids in glycosyltransferases which are strongly involved in determining substrate specificity.
authors.affiliation: Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Freiburg, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Krauth
authors.forename: Marta
authors.initials: M
authors.lastname: Fedoryshyn
authors.forename: Christian
authors.initials: C
authors.lastname: Schleberger
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015202:Protein Engineering; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D000617:Aminoglycosides; C113593:landomycin E; D016695:Glycosyltransferases