title: In vitro priming to direct neuronal fate in adult neural progenitor cells.
abstract: We have previously reported methods that allow for the routine isolation, culture and transplantation of multipotent neural progenitor cells (NPCs) derived from the adult rat sub-ventricular zone (SVZ). In order to expand these techniques, the aim of the current study was to develop an in vitro strategy which can "prime" proliferating adult NPCs towards a neuronal fate during their subsequent differentiation under standard conditions. Our results show that transient exposure to lithium chloride during in vitro proliferation of adult SVZ-derived NPCs has the ability to alter differential fate in vitro and increase the proportion of cells expressing neuronal markers while at the same time causing a reduction in glial progeny. Treatment of adult NPCs with lithium chloride may therefore provide a novel mechanism by which adult NPCs can be primed towards a specific neuronal fate for cell replacement therapy.
authors.affiliation: Department of Pharmacology and Clinical Pharmacology, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Vazey
authors.forename: Bronwen
authors.initials: B
authors.lastname: Connor
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D053687:Adult Stem Cells / Q000187:drug effects / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D064026:Calbindins; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D018021:Lithium Chloride / Q000494:pharmacology; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000494:pharmacology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D014212:Tretinoin; D001973:Bromodeoxyuridine; D018021:Lithium Chloride