title: Mice as potential carriers of infectious bursal disease virus in chickens.
abstract: To investigate the role of mice as potential carriers of infectious bursal disease virus (IBDV), three mice were inoculated with a very virulent strain of IBDV and allowed to have contact with three uninoculated mice. Faeces, intestine and pooled liver and spleen collected from inoculated mice 12 and 24 h post-inoculation were positive for IBDV by reverse transcriptase-polymerase chain reaction (PCR)-nested PCR (RT-PCR-nPCR). IBDV was detected by RT-PCR-nPCR in 3/3 samples of intestine and 2/3 samples of pooled liver and spleen from uninoculated in-contact mice at 24 h after exposure. Chickens developed clinical signs of IBD and died when inoculated with faecal extracts prepared from mice 24 h after inoculation with IBDV. Bursae were atrophied and positive for IBDV by RT-PCR-nPCR.
authors.affiliation: Laboratory of Veterinary Microbiology, School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University,192-1 Hyoza 2 Dong, Chuncheon, Gangwon 200-701, Republic of Korea.
authors.forename: Min Joon
authors.initials: MJ
authors.lastname: Park
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Hyuk Moo
authors.initials: HM
authors.lastname: Kwon
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D002645:Chickens; D004197:Disease Reservoirs / Q000662:veterinary* / Q000821:virology; D007243:Infectious bursal disease virus / Q000302:isolation & purification / Q000472:pathogenicity*; D051379:Mice; D011201:Poultry Diseases / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012376:Rodent Diseases / Q000453:epidemiology / Q000635:transmission* / Q000821:virology