title: Actinomadura maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere soil of Maheshkhali, Bangladesh.
abstract: The taxonomic position of one soil isolate 13-12(50)(T) was clarified by a polyphasic study. The organism showed a combination of chemotaxonomic and morphological properties typical of the genus Actinomadura. It formed a distinct phyletic line in the Actinomadura 16S rRNA gene tree and was closely associated with Actinomadura mexicana (sequence similarity 99.5%), Actinomadura glauciflava, Actinomadura citrea (sequence similarity 99.4%) and Actinomadura madurae (sequence similarity 99.2%). The result of DNA-DNA hybridizations between 13-12(50)(T) and Actinomadura mexicana was 49.9%. On the basis of the phenotypic, chemotaxonomic and genotypic properties, the isolate was differentiated from its closest phylogenetic relatives. It is proposed that the organism be classified as a novel species of the genus Actinomadura. The name proposed for the new taxa are Actinomadura maheshkhaliensis sp. nov. [(13-12(50)(T)=JCM 13934(T) =MTCC 8055(T)].
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Ismet
authors.initials: I
authors.lastname: Ara
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdul Bakir
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001459:Bangladesh; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043982:Rhizophoraceae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S