title: A Candida albicans-specific region of the alpha-pheromone receptor plays a selective role in the white cell pheromone response.
abstract: Candida albicans strains homozygous at the mating type locus can switch from white to opaque, and must do so to mate. Opaque cells then secrete mating pheromones that stimulate opaque cells of opposite mating type to undergo mating. These same pheromones stimulate mating-incompetent white cells to become cohesive and adhesive, and enhance white cell biofilm development, a pathogenic trait. Stimulation is mediated through the same receptor, G protein complex and mitogen-activated protein kinase pathway. Here we present evidence that a C. albicans-specific 55-amino-acid region of the first intracellular loop, IC1, of the alpha-pheromone receptor Ste2p, is required for the alpha-pheromone response of white cells, but not that of opaque cells. This represents a unique regulatory configuration in which activation of a common pathway by the same ligand, the same receptor and the same signal transduction pathway is dependent on a unique region of an intracellular loop of the common receptor in one of the two responding phenotypes.
authors.affiliation: Department of Biology, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Song
authors.initials: S
authors.lastname: Yi
authors.forename: Nidhi
authors.initials: N
authors.lastname: Sahni
authors.forename: Claude
authors.initials: C
authors.lastname: Pujol
authors.forename: Karla J
authors.initials: KJ
authors.lastname: Daniels
authors.forename: Thyagarajan
authors.initials: T
authors.lastname: Srikantha
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: David R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D000595:Amino Acid Sequence; D018441:Biofilms; D002176:Candida albicans / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D049770:Genes, Mating Type, Fungal; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D010675:Pheromones / Q000378:metabolism*; D051964:Receptors, Mating Factor / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017384:Sequence Deletion; D015398:Signal Transduction
chemical_list: D005656:Fungal Proteins; D010675:Pheromones; D051964:Receptors, Mating Factor; D020928:Mitogen-Activated Protein Kinases