title: Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing.
abstract: Materials able to deliver topically bioactive molecules represent a new generation of biomaterials. In this article, we describe the use of silk mats, made of electrospun nanoscale silk fibers containing epidermal growth factor (EGF), for the promotion of wound healing processes. In our experiments, we demonstrated that EGF is incorporated into the silk mats and slowly released in a time-dependent manner (25% EGF release in 170h). We tested these materials using a new model of wounded human skin-equivalents displaying the same structure as human skin and able to heal using the same molecular and cellular mechanisms found in vivo. This human three-dimensional model allows us to demonstrate that the biofunctionalized silk mats, when placed on the wounds as a dressing, aid the healing by increasing the time of wound closure by the epidermal tongue by 90%. The preservation of the structure of the mats during the healing period as demonstrated by electronic microscopy, the biological action of the dressing, as well as the biocompatibility of the silk demonstrate that this biomaterial is a new and very promising material for medical applications, especially for patients suffering from chronic wounds.
authors.affiliation: Division of Cancer Biology and Tissue Engineering, Department of Oral and Maxillofacial Pathology, Tufts University, School of Dental Medicine, 55 Kneeland Street, Boston, MA 02111, USA.
authors.forename: A
authors.initials: A
authors.lastname: Schneider
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: D L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: J A
authors.initials: JA
authors.lastname: Garlick
authors.forename: C
authors.initials: C
authors.lastname: Egles
mesh_terms: D000042:Absorption; D000287:Administration, Topical; D001458:Bandages; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004815:Epidermal Growth Factor / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D012399:Rotation; D047011:Silk / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000293:injuries*; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000628:therapy*
chemical_list: D004337:Drug Carriers; D047011:Silk; D004815:Epidermal Growth Factor