title: [Apoptosis of laryngeal carcinoma cells induced by antisense oligodeoxynucleotides complementary Stat3 in vitro].
abstract: OBJECTIVE
  To study the effects of oligodeoxynucleotides complementary Stat3 on apoptosis in laryngeal carcinoma Hep-2 cell.
  
  
  METHODS
  Oligodeoxynucleotides complementary Stat3 was designed, which was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Expression of Stat3 and p-Stat3 were detected by Western blot and PCR. MTT was used to observe the growth-inhibiting ratio. DNA ladder, AO/EB and FCM were used to observe the apoptosis of laryngeal carcinoma cell Hep-2 in vitro.
  
  
  RESULTS
  Western blot and PCR results demonstrated that oligodeoxynucleotides complementary Stat3 could significantly inhibit the expression of Stat3 and p-Stat3 in Hep-2 cell. MTT results showed that it could significantly suppress the growth of Hep-2 cell. The DNA ladder, AO/EB and FCM results showed it could inhibit the expression of Stat3 and induce the apoptosis of Hep-2 cell in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Oligodeoxynucleotides complementary Stat3 could induce the apoptosis and suppress cell proliferation in laryngeal carcinoma Hep-2 cell.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital University of Medical Science, Beijing, 100053, China. lvhaili@hotmail.com
authors.forename: Haili
authors.initials: H
authors.lastname: Lü
authors.forename: Qiuhang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000494:pharmacology*; D050796:STAT3 Transcription Factor / Q000235:genetics*; D014162:Transfection
chemical_list: D009838:Oligodeoxyribonucleotides; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human