Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil.
A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity.
Lladó
Salvador
S
Department of Microbiology, University of Barcelona, Diagonal 645, E-08028, Barcelona, Spain.
Jiménez
Nuria
N
Viñas
Marc
M
Solanas
Anna Maria
AM
D041963:Bacteroidetes / Q000378:metabolism; D001483:Base Sequence; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002911:Chrysenes / Q000378:metabolism*; D003407:Creosote / Q000378:metabolism; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D001551:Benz(a)Anthracenes; D002911:Chrysenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C031180:chrysene; D003407:Creosote; C030935:benz(a)anthracene