title: Bioaerosols chemometric characterization by laser-induced fluorescence: air sample analysis.
abstract: A laser-induced fluorescence (LIF) system was optimized using a solution of Micrococcus luteus in ethanol/water 50% (v/v) to obtain spectra in the gas phase of 46 bioaerosols. Experimental designs such as Plackett-Burman and factorial design were applied. The fluorescence spectra were treated chemometrically by principal component analysis, linear discriminant analysis and hierarchical cluster analysis to classify the microorganisms according to family, morphology and gram. The best results were obtained using LDA. The method was applied to air samples and the LIF results allowed to characterize bioaerosols reliability. The robustness of the technique was demonstrated by the identification of many bacteria.
authors.affiliation: Chemistry Department (Analytical Chemistry Area), University of La Rioja, C/ Madre de Dios 51, 26006 Logroño (La Rioja), Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Cabredo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Parra
authors.forename: Cecilia
authors.initials: C
authors.lastname: Sáenz
authors.forename: Jesús
authors.initials: J
authors.lastname: Anzano
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D005740:Gases; D016982:Micrococcus luteus; D025341:Principal Component Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005740:Gases