title: Short-term effect of topical dorzolamide hydrochloride on intrastromal corneal pressure in rabbit corneas in vivo.
abstract: OBJECTIVE
  To evaluate the effect of topical dorzolamide on the intrastromal corneal pressure (ICP) in rabbit corneas in vivo.
  
  
  METHODS
  This is an interventional prospective study. Topical dorzolamide was applied to 7 eyes of 7 male New Zealand rabbits 3 times daily for 3 consecutive days. The ICP changes were recorded with a pressure transducer connected to the midperipheral cornea. The ICP was measured in the same manner in 7 eyes of 7 male New Zealand rabbits that were treated with artificial tears (control group).
  
  
  RESULTS
  The ICP values averaged -6.2 +/- 3.2, -10 +/- 5.8, and -12.5 +/- 8.7 mm Hg at 15, 30, and 45 minutes in the control group, respectively. In the dorzolamide-treated eyes, the ICP readings were 1.8 +/- 3.4, -0.28 +/- 4.3, and -1.8 +/- 5.3 mm Hg at the same time points, respectively. The differences in the ICP between both groups were significantly different at all time points (P = 0.004, P = 0.005, and P = 0.02).
  
  
  CONCLUSIONS
  Measuring ICP is a valid and sensitive method to evaluate in vivo the endothelial function. This method seems to be more sensitive than measuring the central corneal thickness or the corneal deswelling rate in detecting changes in the corneal physiology with the use of topical dorzolamide.
authors.affiliation: Hospital Universitario Príncipe de Asturias, Alcalá de Henares, Madrid, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Teus
authors.forename: Gema
authors.initials: G
authors.lastname: Bolívar
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Alió
authors.forename: Isaac
authors.initials: I
authors.lastname: Lipshitz
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000008:administration & dosage*; D003319:Corneal Stroma / Q000187:drug effects* / Q000502:physiology; D004334:Drug Administration Schedule; D008297:Male; D011312:Pressure; D011817:Rabbits; D013449:Sulfonamides / Q000008:administration & dosage* / Q000494:pharmacology; D013876:Thiophenes / Q000008:administration & dosage* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D013449:Sulfonamides; D013876:Thiophenes; C062765:dorzolamide