title: Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells.
abstract: Exposure to high levels of ozone (O(3)) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O(3)-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O(3)-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O(3) and/or O(3)-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O(3) and/or its cytotoxic products and may provide protections against O(3)-induced biotoxicities.
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Castagna
authors.forename: P A
authors.initials: PA
authors.lastname: Davis
authors.forename: V T
authors.initials: VT
authors.lastname: Vasu
authors.forename: K
authors.initials: K
authors.lastname: Soucek
authors.forename: C E
authors.initials: CE
authors.lastname: Cross
authors.forename: L
authors.initials: L
authors.lastname: Greci
authors.forename: G
authors.initials: G
authors.lastname: Valacchi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D005609:Free Radicals; D016209:Interleukin-8; D010083:Oxidants, Photochemical; D012333:RNA, Messenger; D010126:Ozone; C003959:TEMPO