Immunological assessment of plant-derived avian flu H5/HA1 variants.
Polypeptide variants of the HA1 antigenic domain of the H5N1 avian influenza virus hemagglutinin (HA) molecule were produced in plants using transient and stable expression systems and fused with His/c-myc tags or with mouse or human Fc antibody fragments. The resulting peptides were purified and used for intramuscular immunization of mice. While the recombinant HA1 variants induced a significant serum humoral immune response in the mice, none of the HA1 preparations induced virus-neutralizing antibodies. Fusion with the Fc fragment improved overall yield of the constructs and allowed purification requiring only a single step, but led to no detectable fusion-related enhancement of immunogenicity or quality of immune response.
Spitsin
S
S
Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107-6799, United States.
Andrianov
V
V
Pogrebnyak
N
N
Smirnov
Y
Y
Borisjuk
N
N
Portocarrero
C
C
Veguilla
V
V
Koprowski
H
H
Golovkin
M
M
D000818:Animals; D000917:Antibody Formation; D001717:Birds; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000276:immunology*
D007252:Influenza Vaccines; D010940:Plant Proteins