Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis.
The velogenic Newcastle disease virus (NDV) causes highly infectious and economically significant Newcastle disease (ND) in birds of various species. In cell culture NDV induces cytopathic effect (CPE) characterized by rounding, vacuolation, syncytia formation and cell death. Aside from cell to cell fusion caused by the F and HN glycoprotein of the virus molecular events leading to cell death are not known. In the current study, NDV-infected Vero cells, at 48 h p.i., showed nuclear condensation, cytoplasm blebbing, DNA fragmentation, and phosphatidylserine translocation to the cell surface. In addition, virus-infected cells demonstrated decreased DNA content and an increased Bax to Bcl-2 ratio, p53 level and caspase 3, 8, 9 expression compared to mock-infected cells. Based on these results, it was concluded that CPE in NDV-infected cells was caused by to the induction of apoptosis with the involvement of p53 and the Bax, dependent apoptotic pathways.
Ravindra
P V
PV
Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122 UP, India. raviravindra1@gmail.com
Tiwari
Ashok K
AK
Ratta
Barkha
B
Chaturvedi
Uttara
U
Palia
Sudesh Kumar
SK
Chauhan
R S
RS
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D053938:DNA Fragmentation; D015870:Gene Expression; D009521:Newcastle Disease / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology* / Q000821:virology*; D009522:Newcastle disease virus / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells
D019253:Proto-Oncogene Proteins c-bcl-2; D020169:Caspases