title: Effects of wheat albumin consumption on expression of genes related to lipogenesis and insulin sensitivity in adipose tissues of rats.
abstract: Suppression of postprandial hyperglycemia reduces lipogenic enzyme activities in the adipose tissues of normal rats. The present study investigated the expression of genes related to lipogenesis and insulin sensitivity in mesenteric adipose and epididymal adipose tissues to evaluate the effects of wheat albumin (WA) and a crude preparation of WA (CWA) with alpha-amylase inhibitory activity on lipid metabolism. Rats fed 2.5% WA, which had 12.7-fold inhibitory activity compared with CWA, exhibited reduced mRNA levels for G6PDH, ACO, ACS, PEPCK, and LPL in the mesenteric adipose, but not in the epididymal adipose tissue. Linear regression analyses showed that the gene expression levels of FAS, G6PDH, ACS, and LPL were reduced in dose-dependent manners in the mesenteric adipose tissue of rats fed the CWA diet. These results suggest that supplementation with CWA as well as WA reduces the expression of genes related to lipogenesis and insulin sensitivity in mesenteric adipose tissue.
authors.affiliation: Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences and Global COE Program, The University of Shizuoka, Shizuoka, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Murayama
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Masaya
authors.initials: M
authors.lastname: Shimada
authors.forename: Yuki
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Kenji
authors.initials: K
authors.lastname: Shibata
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000418:Albumins / Q000008:administration & dosage*; D000818:Animals; D004032:Diet; D015870:Gene Expression / Q000187:drug effects; D007333:Insulin Resistance / Q000235:genetics*; D050155:Lipogenesis / Q000235:genetics*; D008297:Male; D008643:Mesentery; D010940:Plant Proteins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D014908:Triticum / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000418:Albumins; D010940:Plant Proteins; D000516:alpha-Amylases