title: Peptide-functionalized microfabricated structures for improved on-chip neuronal adhesion.
abstract: Extracellular, high signal-to-noise ratio recordings from electrogenic cells require a tight coupling between the cellular membrane and the recording electrode. Self assembled monolayers (SAMs) of alkanethiols functionalized with peptides were used in combination with micro- and nano-structured features on the sensor surface. This combination of surface chemistry and topography triggers a phagocytosis-like engulfment and ensures tight coupling. In this paper we report the results concerning usage of different SAMs and the influence of the peptide concentration towards cell adhesion and outgrowth. Later on, the optimized peptide functionalized SAMs were applied on micro- and nano-structured sensor surfaces. As a result, phagocytosis-like events could be shown using focused ion beam SEM and confocal fluorescence imaging.
authors.affiliation: Dept. of Chemistry of the KU Leuven, Flemish Institute of Biotechnology (VIB), Belgium. bart.vanmeerbergen@imec.be
authors.forename: B
authors.initials: B
authors.lastname: Van Meerbergen
authors.forename: K
authors.initials: K
authors.lastname: Jans
authors.forename: J
authors.initials: J
authors.lastname: Loo
authors.forename: G
authors.initials: G
authors.lastname: Reekmans
authors.forename: D
authors.initials: D
authors.lastname: Braeken
authors.forename: C
authors.initials: C
authors.lastname: Seon-Ah
authors.forename: K
authors.initials: K
authors.lastname: Bonroy
authors.forename: G
authors.initials: G
authors.lastname: Maes
authors.forename: G
authors.initials: G
authors.lastname: Borghs
authors.forename: Y
authors.initials: Y
authors.lastname: Engelborghs
authors.forename: W
authors.initials: W
authors.lastname: Annaert
authors.forename: C
authors.initials: C
authors.lastname: Bartic
mesh_terms: D000473:Alkanes; D000818:Animals; D001698:Biomedical Engineering; D015374:Biosensing Techniques; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D004566:Electrodes; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010455:Peptides; D013438:Sulfhydryl Compounds; D013499:Surface Properties
chemical_list: D000473:Alkanes; D010455:Peptides; D013438:Sulfhydryl Compounds