[Determination content of the antidepressant extraction and analysis the trace elements from Morinda officinalis].
OBJECTIVE
To study on the antidepressive extraction and determinated the content of the oligosaccharides I , the proteins and the trace elements from Morinda officinalis.


METHODS
We analyzed the content of the total oligosaccharides, the proteins and the trace elements by Phenol-Sulferic acid, Coomassie brilliant blue and inductive coupling plasm atomic emission spectrometer (ICP-AES) methods, respectively.


RESULTS
The percent of the oligosaccharides and the proteins were 89.4% and 0.19% in the extraction from M. officinalis, respectively. The total actively oligosaccharides I were subjected to the D-941 ion column chromatography to give the extraction in which hardly and protein were determined. 12 kinds of trace element including Zn, Fe, Ca ect. in the extraction (the oligosaccharides I) were determined by ICP-AES without the noxious trace elements including As, Cd, Pb ect.


CONCLUSIONS
The antidepressive activity extraction(the oligosaccharides I ) was a highly pure and vegetal oligosaccharides.
Li
Jun
J
Institute of Natural Medicine and TCM, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China.
Zhang
Hua-Lin
HL
Wang
Zhe
Z
Liang
Yu-Ming
YM
Jiang
Lin
L
Ma
Wei
W
Yang
De-Po
DP
D000928:Antidepressive Agents / Q000737:chemistry* / Q000302:isolation & purification; D032066:Morinda / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D013054:Spectrophotometry, Atomic / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014131:Trace Elements / Q000032:analysis*
D000928:Antidepressive Agents; D009844:Oligosaccharides; D011506:Proteins; D014131:Trace Elements