title: Programmed subcellular release for studying the dynamics of cell detachment.
abstract: Cell detachment is central to a broad range of physiopathological changes, but there are no quantitative methods to study this process. Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level.
authors.affiliation: Department of Materials Science and Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA.
authors.forename: Bridget
authors.initials: B
authors.lastname: Wildt
authors.forename: Denis
authors.initials: D
authors.lastname: Wirtz
authors.forename: Peter C
authors.initials: PC
authors.lastname: Searson
mesh_terms: D016475:3T3 Cells; D036002:ADP Ribose Transferases / Q000494:pharmacology; D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology; D000205:Actomyosin / Q000502:physiology; D000465:Algorithms; D000818:Animals; D001381:Azepines / Q000494:pharmacology; D001905:Botulinum Toxins / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D004558:Electric Stimulation; D004566:Electrodes; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D022001:Focal Adhesions / Q000187:drug effects / Q000502:physiology; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D009281:Naphthalenes / Q000494:pharmacology; D009842:Oligopeptides / Q000737:chemistry; D022502:Stress Fibers / Q000502:physiology; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D001381:Azepines; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004791:Enzyme Inhibitors; D006576:Heterocyclic Compounds, 4 or More Rings; D009281:Naphthalenes; D009842:Oligopeptides; D053778:Thiazolidines; C070571:ML 7; C472645:blebbistatin; D006046:Gold; C047981:arginyl-glycyl-aspartic acid; D000205:Actomyosin; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; C037068:latrunculin B