[Molecular subtyping of Staphylococcus aureus isolated from a severe food-poisoning].
OBJECTIVE
To study the molecular types of Staphylococcus aureus isolated from a severe food-poisoning and to trace the possible strains.


METHODS
Real-time PCR was applied to detect nuc gene as a specific marker for S. aureus, mecA gene encoding methicillin resistance and 5 other genes encoding staphylococcal enterotoxins (sea, seb, see, sed, see). Isolates were also performed with 16S rRNA oligonucleotide sequence analyzing by DNAStar MegAlign 5.0 software and pulse-field gel electrophoresis (PFGE) by BioNumerics Version 4.0 software.


RESULTS
The nuc gene was detected from the 10 isolated strains, sea and seb genes were detected from 7 strains. There were 4 16 S rRNA types and 5 PFGE types found from all the strains.


CONCLUSIONS
Three relative S. aureus strains were involved in the severe food-poisoning at least. Molecular subtyping might give a molecular epidemiological evidence and support the source tracing of an outbreak.
Zhang
Ying
Y
Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
Mo
Zi-Yao
ZY
Pang
Xing-Lin
XL
Deng
Zhi-Ai
ZA
Zhang
Xin-Qiang
XQ
Chen
Shou-Yi
SY
Wang
Ming
M
D015373:Bacterial Typing Techniques; D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins; D006801:Humans; D013202:Staphylococcal Food Poisoning / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004768:Enterotoxins