title: [Survey on human rabies cases and its viral molecular biological features in Baoshan city, Yunnan province].
abstract: OBJECTIVE
  To understand the epidemiological features of two rabies cases in Baoshan city year 2006 and 2007 and to analyze its source of infection.
  
  
  METHODS
  Questionnaires were used to do the epidemiological survey on each of the rabies cases. Brain tissue samples of rabies patients were collect to detect the rabies virus by direct immunofluorescence assay (DFA) and RT-PCR assay. Homology and phylogenetic tree were analyzed, based on the whole nucleotide and deduced amino acid sequence of P, M and N gene of rabies virus followed by molecular epidemiological analysis.
  
  
  RESULTS
  In July 2006, one human rabies case was identified in Longyang district, and another one in Tengchong county in Baoshan city in 2007. The degrees of exposure of these two patients was all at degree III. Two brain tissue samples among the dead patients (No. CYN0601H and CYN0701H) were confirmed positive by both DFA and RT-PCR assay. The homology analysis of P, M and N gene sequences among CYN0601H, CYN0701H and other rabies strains isolated from other provinces and other counties, showed that the samples in Baoshan city shared the highest homology with the strains in Thailand. Phylogenetic analysis indicated that the two samples were very close and all belonged to genetype 1 Lyssavirus, with the closest relationship between samples in Baoshan city and strains in Thailand.
  
  
  CONCLUSIONS
  It was confirmed on the virus molecular level that the two patients in Baoshan city were both suffered from rabies. The prevalent strains in Baoshan city was probably imported from foreign country, suggesting that prevention and control measures on rabies virus in the boarder areas of Yunnan should be strengthened.
authors.affiliation: Yunnan Provincal Institute of Endemic Disease Control and Prevention, Dali 671000, China.
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Tao
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Yun-Zhi
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Yang
authors.forename: Zhu-Qing
authors.initials: ZQ
authors.lastname: Mi
authors.forename: Jing-Lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Zheng
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Lu
authors.forename: Sheng-Guo
authors.initials: SG
authors.lastname: Li
mesh_terms: D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA