title: Antiosteoporotic activity of anthraquinones from Morinda officinalis on osteoblasts and osteoclasts.
abstract: Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1), rubiadin-1-methyl ether (2), 2-hydroxy-1-methoxy- anthraquinone (3), 1,2-dihydroxy-3-methylanthraquinone (4), 1,3,8-trihydroxy-2-methoxy- anthraquinone (5), 2-hydroxymethyl-3-hydroxyanthraquinone (6), 2-methoxyanthraquinone (7) and scopoletin (8) from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Among them, compounds 2 and 3 promoted osteoblast proliferation, while compounds 4, 5 increased osteoblast ALP activity. All of the isolated compounds inhibited osteoclast TRAP activity and bone resorption, and the inhibitory effects on osteoclastic bone resorption of compounds 1 and 5 were stronger than that of other compounds. Taken together, antiosteoporotic activity of M. officinalis and its anthraquinones suggest therapeutic potential against osteoporosis.
authors.affiliation: Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, PR China. wxsq1@163.com
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Wu
authors.forename: Cheng-Jian
authors.initials: CJ
authors.lastname: Zheng
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Qin
authors.forename: Lian-Na
authors.initials: LN
authors.lastname: Sun
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Jiao
authors.forename: Qiao-Yan
authors.initials: QY
authors.lastname: Zhang
authors.forename: Jin-Zhong
authors.initials: JZ
authors.lastname: Wu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D050071:Bone Density Conservation Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D001862:Bone Resorption / Q000188:drug therapy; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D015394:Molecular Structure; D032066:Morinda / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000880:Anthraquinones; D050071:Bone Density Conservation Agents; D000469:Alkaline Phosphatase