title: F-type lectin from the sea bass (Dicentrarchus labrax): purification, cDNA cloning, tissue expression and localization, and opsonic activity.
abstract: Recently described biochemical and structural aspects of fucose-binding lectins from the European eel (Anguilla anguilla) and striped bass (Morone saxatilis) led to the identification of a novel lectin family ("F-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. The F-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. Here we describe the purification, biochemical and molecular properties, and the opsonic activity of an F-type lectin (DlFBL) isolated from sea bass (Dicentrarchus labrax) serum. DlFBL exhibits two tandemly arranged carbohydrate-recognition domains that display the F-type sequence motif. In situ hybridization and immunohistochemical analysis revealed that DlFBL is specifically expressed and localized in hepatocytes and intestinal cells. Exposure of formalin-killed Escherichia coli to DlFBL enhanced their phagocytosis by D. labrax peritoneal macrophages relative to the unexposed controls, suggesting that DlFBL may function as an opsonin in plasma and intestinal mucus.
authors.affiliation: Marine Immunobiology Laboratory, Department of Animal Biology, University of Palermo, Via Archirafi 18, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Salerno
authors.forename: M G
authors.initials: MG
authors.lastname: Parisi
authors.forename: D
authors.initials: D
authors.lastname: Parrinello
authors.forename: G
authors.initials: G
authors.lastname: Benenati
authors.forename: A
authors.initials: A
authors.lastname: Vizzini
authors.forename: M
authors.initials: M
authors.lastname: Vazzana
authors.forename: G R
authors.initials: GR
authors.lastname: Vasta
authors.forename: M
authors.initials: M
authors.lastname: Cammarata
mesh_terms: D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000378:metabolism*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D015151:Immunoblotting; D037102:Lectins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D009895:Opsonin Proteins / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D009895:Opsonin Proteins; D012333:RNA, Messenger