title: Purification, crystallization and preliminary X-ray diffraction analysis of glutathionylated Trx1 C33S mutant from yeast.
abstract: Thioredoxins (Trxs) are a family of small redox-active proteins that are found in all living organisms. In Saccharomyces cerevisiae, two cytosolic Trxs (Trx1 and Trx2) and one mitochondrial Trx (Trx3) have previously been identified. In this work, cytosolic Trx1 containing a C33S mutant was overexpressed, purified, glutathionylated and crystallized using the hanging-drop vapour-diffusion method. A set of X-ray diffraction data was collected to 1.80 A resolution. The crystal belonged to space group P1, with unit-cell parameters a = 38.53, b = 38.81, c = 41.70 A, alpha = 72.91, beta = 87.51, gamma = 60.58 degrees.
authors.affiliation: Institute of Protein Research, Tongji University, Shanghai, People's Republic of China.
authors.forename: Xiaochu
authors.initials: X
authors.lastname: Lou
authors.forename: Yaru
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Bao
authors.forename: Cong-Zhao
authors.initials: CZ
authors.lastname: Zhou
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D020134:Catalytic Domain; D003001:Cloning, Molecular; D003460:Crystallization; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D008956:Models, Chemical; D009154:Mutation; D054464:Peroxiredoxins; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013879:Thioredoxins / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents; C103243:TRX1 protein, S cerevisiae; C516110:TRX2 protein, S cerevisiae; D013879:Thioredoxins; D054464:Peroxiredoxins; D005978:Glutathione