Expression of inducible nitric oxide synthase and vascular endothelial growth factor in ameloblastoma.
The purposes of this study were to determine the correlation between the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) in ameloblastoma and to examine the relationships of this expression to angiogenesis and the clinical and biological behaviors of the tumor. Immunohistochemical staining with streptavidin peroxidase was used to analyze iNOS and VEGF expression, and CD34 was used to evaluate microvascular density (MVD) in 35 ameloblastomas (24 primary tumors and 11 recurrences) and 5 malignant ameloblastomas. Ten odontogenic keratocysts (OKCs) served as controls. On relational analysis, positive and VEGF expression and MVD counts increased in this order: OKCs, primary ameloblastoma, recurrent ameloblastoma, and malignant ameloblastoma. Differences between the ameloblastomas and OKCs were significant (P < 0.05). Among ameloblastomas, MVD counts increased with increasing expression of iNOS and VEGF (P < 0.05), and iNOS expression and VEGF expression were positively correlated (r = 0.66, P < 0.05). Inducible nitric oxide synthase expression and VEGF expression may be closely related to the angiogenesis and invasive biological behavior of ameloblastomas.
Chen
Wei-liang
WL
Department of Oral and Maxillofacial Surgery, Center of Cranio-Maxillofacial Surgery, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. drchen@vip.136.com
Ouyang
Ke-xiong
KX
Li
Hai-gang
HG
Huang
Zhi-quan
ZQ
Li
Jing-son
JS
Wang
Jian-guang
JG
D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000098:blood supply / Q000473:pathology*; D018952:Antigens, CD34 / Q000032:analysis; D002196:Capillaries / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D055806:Microvessels / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology*; D052247:Nitric Oxide Synthase Type II / Q000032:analysis*; D009807:Odontogenic Cysts / Q000098:blood supply / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D055815:Young Adult
D018952:Antigens, CD34; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II