Sciscionella marina gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea.
The taxonomic position of an actinomycete, designated SCSIO 00231(T), isolated from a sediment sample collected from the northern South China Sea, was determined by using a polyphasic approach. The organism formed fragmented substrate hyphae and sparse aerial mycelium on modified ISP 2 medium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCSIO 00231(T) fell into the family Pseudonocardiaceae, in which it formed a distinct lineage and was loosely associated with Thermocrispum municipale DSM 44069(T), with 93 % similarity. The other closest phylogenetic neighbours were Saccharopolyspora erythraea NRRL 2338(T) (92.6 % similarity) and Amycolatopsis sacchari DSM 44468(T) (93.1 % similarity). The isolate had cell-wall type IV (meso-diaminopimelic acid and whole-cell sugars arabinose, galactose and glucose) and phospholipid type III. The predominant menaquinone was MK-9(H(4)). The G+C content of the genomic DNA was 69 mol%. Based on these data, strain SCSIO 00231(T) can be readily distinguished from previously described organisms and represents a new genus within the family Pseudonocardiaceae. The name Sciscionella gen. nov. is proposed, with the novel species Sciscionella marina sp. nov. The type strain of Sciscionella marina is SCSIO 00231(T) (=KCTC 19433(T) =CCTCC AA208009(T)).
Tian
Xin-Peng
XP
Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Zhi
Xiao-Yang
XY
Qiu
Yun-Qi
YQ
Zhang
Yu-Qin
YQ
Tang
Shu-Kun
SK
Xu
Li-Hua
LH
Zhang
Si
S
Li
Wen-Jun
WJ
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002473:Cell Wall / Q000737:chemistry; D002681:China; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D012336:RNA, Ribosomal, 16S