title: Detection of acetyl monoglyceride as a metabolite of newly isolated glycerol-assimilating bacteria.
abstract: Thirty-five glycerol-assimilating bacteria have recently been isolated from soil samples. Amplified ribosomal DNA restriction analysis revealed that these strains are grouped into four genetically different types of bacteria. Gas chromatography-mass spectrometry analysis of glycerol metabolites produced by the three selected strains (strains HH7, HH12, and HH31) revealed that extracts of culture liquid with ethyl acetate contains acetyl monoglyceride (monoacetin), which has not previously been reported as a glycerol metabolite and is used as a solvent, plasticizer, and food additive, as well as for other industrial purposes. The sequence analyses of the 16S rRNA genes from the selected strains showed that all of them belong to the Enterobacteriaceae.
authors.affiliation: Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. hiroshi.habe@aist.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwabuchi
authors.forename: Shingo
authors.initials: S
authors.lastname: Uemura
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tamura
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Keiji
authors.initials: K
authors.lastname: Sakaki
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D004755:Enterobacteriaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005503:Food Additives / Q000378:metabolism*; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012997:Solvents / Q000378:metabolism*
chemical_list: D005503:Food Additives; D005989:Glycerides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012997:Solvents; C038923:monoacetin; D005990:Glycerol