Characterization of chryseobacterium aquaticum strain PUPC1 producing a novel antifungal protease from rice rhizosphere soil.
Strain PUPC1 produces an antifungal protease as well as plant growth promoting enzymes such as 1- aminocyclopropane-1-carboxylate (ACC) deaminase and phosphatase. Morphological, cultural, and physiological characteristics as well as 16S rRNA gene-sequence-based phylogenetic analysis confirmed the taxonomic affiliation of PUPC1 as Chryseobacterium aquaticum. The optimum growth of PUPC1 was observed at pH 6.0 and 30 degrees , and maximum protease production was observed in medium B amended with 1% tryptone, 0.5% sucrose, and 0.005% MnCl2. The protease was purified by ammonium sulfate precipitation, Sephadex G-75 gel filtration chromatography, and electroelution from preparative SDS-PAGE. The protease had a molecular mass of 18.5 kDa. The optimum pH and temperature stability of the protease were pH 5.0- 10.0 and temperature 40-70 degrees . Chryseobacterium aquaticum PUPC1 and its protease showed a broad-spectrum antifungal activity against phytopathogenic fungi. Strain PUPC1 also exhibited plant growth promoting traits. The objective of the present investigation was to isolate a strain for agricultural application for plant growth promotion and biocontrol of fungal diseases.
Gandhi Pragash
M
M
Department of Biotechnology, Pondicherry University, Kalapet, Puducherry, India.
Narayanan
K Badri
KB
Naik
P Ravindra
PR
Sakthivel
N
N
D000935:Antifungal Agents / Q000302:isolation & purification*; D019755:Carbon-Carbon Lyases / Q000096:biosynthesis; D045247:Chryseobacterium / Q000201:enzymology* / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial; D006863:Hydrogen-Ion Concentration; D012275:Oryza / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature
D000935:Antifungal Agents; D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010744:Phosphoric Monoester Hydrolases; D010447:Peptide Hydrolases; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases