title: Evaluation of interferences between dengue vaccine serotypes in a monkey model.
abstract: Interferences between different antigens in the same vaccine formulation have been reported for some vaccines (e.g., polio vaccines, live attenuated dengue vaccine candidates). We examined interferences between the four serotypes of ChimeriVax dengue vaccines (CYDs) in a monkey model when present within a tetravalent formulation in equal concentrations (TV-5555). Immunoassays of vaccinated non-human primates showed that serotype 4 (DEN-4), and to a lesser extent, DEN-1 were dominant in terms of neutralizing antibody levels. Parameters that affected the interferences were identified, including 1) the simultaneous administration of two complementary bivalent vaccines at separate anatomical sites drained by different lymph nodes; 2) the sequential administration of two complementary bivalent vaccines; 3) the establishment of heterologous flavivirus pre-immunity before subsequent tetravalent immunization; 4) the adaptation of formulations by decreasing the dose of the immunodominant serotype; and 5) the administration of a 1-year booster. The applicability of these data to human responses is discussed.
authors.affiliation: Research and Discovery Departments, Sanofi Pasteur, Marcy l'Etoile, France. bruno.guy@sanofipasteur.com
authors.forename: Bruno
authors.initials: B
authors.lastname: Guy
authors.forename: Veronique
authors.initials: V
authors.lastname: Barban
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mantel
authors.forename: Marion
authors.initials: M
authors.lastname: Aguirre
authors.forename: Sandrine
authors.initials: S
authors.lastname: Gulia
authors.forename: Jeremy
authors.initials: J
authors.lastname: Pontvianne
authors.forename: Therese-Marie
authors.initials: TM
authors.lastname: Jourdier
authors.forename: Laurence
authors.initials: L
authors.lastname: Ramirez
authors.forename: Veronique
authors.initials: V
authors.lastname: Gregoire
authors.forename: Christophe
authors.initials: C
authors.lastname: Charnay
authors.forename: Nicolas
authors.initials: N
authors.lastname: Burdin
authors.forename: Rafaele
authors.initials: R
authors.lastname: Dumas
authors.forename: Jean
authors.initials: J
authors.lastname: Lang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003715:Dengue / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D053059:Dengue Vaccines / Q000008:administration & dosage / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification* / Q000276:immunology* / Q000472:pathogenicity; D004195:Disease Models, Animal; D006801:Humans; D007115:Immunization Schedule; D007117:Immunization, Secondary; D008252:Macaca fascicularis; D008297:Male; D009500:Neutralization Tests; D012703:Serotyping; D014611:Vaccination; D014709:Vero Cells; D014762:Viral Interference; D014766:Viremia / Q000276:immunology / Q000517:prevention & control / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D053059:Dengue Vaccines