title: Recombinant measles viruses expressing single or multiple antigens of human immunodeficiency virus (HIV-1) induce cellular and humoral immune responses.
abstract: Recombinant measles viruses (rMV) based on the live attenuated measles vaccine strain (MVb) expressing antigens of HIV-1 clade B were generated by reverse genetics. Recombinants expressing single or double antigens of HIV-1 (rMV-HIV) were genetically highly stable on human diploid cells. The production process of these viruses was essentially similar to the parental MV strain, yielding comparative end titers. Immunization of tg-mice by different regimens and formulations showed potent humoral and cellular immune responses against MV and HIV antigens. Recombinant MV-HIV expressing Gag protein conferred protective immunity in tg-mice after a high-dose pseudochallenge with recombinant vaccinia virus. In addition, rMV-HIV boosted anti-HIV antibodies, in the presence of pre-existing anti-vector antibodies.
authors.affiliation: Crucell - Berna Biotech LTD, Rehhagstrasse 79, 3018 Bern, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Liniger
authors.forename: Armando
authors.initials: A
authors.lastname: Zuniga
authors.forename: Teldja Neige Azzouz
authors.initials: TN
authors.lastname: Morin
authors.forename: Behazine
authors.initials: B
authors.lastname: Combardiere
authors.forename: Rene
authors.initials: R
authors.lastname: Marty
authors.forename: Marian
authors.initials: M
authors.lastname: Wiegand
authors.forename: Orhan
authors.initials: O
authors.lastname: Ilter
authors.forename: Marlyse
authors.initials: M
authors.lastname: Knuchel
authors.forename: Hussein Y
authors.initials: HY
authors.lastname: Naim
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000096:biosynthesis; D019076:Transgenes; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015488:HIV Antigens; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D007371:Interferon-gamma