Effects of cadmium on respiratory burst, intracellular Ca2+ and DNA damage in the white shrimp Litopenaeus vannamei.
Acute effects of heavy metal ions on shrimp have been an area of intense study worldwide. However, the molecular mechanism by which cadmium-induced injury occurs remains largely unclear, and methods for mitigating toxicity in vivo have rarely been reported. In this study, the changes in respiratory burst and intracellular free calcium in haemocytes of pacific white shrimp, Litopenaeus vannamei, after exposure to Cd(2+) (CdCl(2)) were examined using flow cytometry. Meanwhile, DNA damage and repair in haemocytes and hepatopancreas cells were studied using the comet assay. Respiratory burst generation, intracellular Ca(2+) concentration ([Ca(2+)]i) and DNA damage in haemocytes and hepatopancreas cells all exhibited a dose-dependent increase and a time-dependent change after treatment with Cd(2+) compared with controls. These results indicate that Cd can induce oxidative stress and DNA damage in the shrimp L. vannamei. Moreover, the results also demonstrate that these parameters can be used as sensitive indicators of exposure to this genotoxicant.
Chang
Ming
M
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
Wang
Wei-Na
WN
Wang
An-Li
AL
Tian
Ting-Ting
TT
Wang
Peng
P
Zheng
Ying
Y
Liu
Yuan
Y
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D033561:Penaeidae / Q000187:drug effects* / Q000502:physiology; D016897:Respiratory Burst / Q000187:drug effects*
D002104:Cadmium; D002118:Calcium