title: Mega-phylogeny approach for comparative biology: an alternative to supertree and supermatrix approaches.
abstract: BACKGROUND
  Biology has increasingly recognized the necessity to build and utilize larger phylogenies to address broad evolutionary questions. Large phylogenies have facilitated the discovery of differential rates of molecular evolution between trees and herbs. They have helped us understand the diversification patterns of mammals as well as the patterns of seed evolution. In addition to these broad evolutionary questions there is increasing awareness of the importance of large phylogenies for addressing conservation issues such as biodiversity hotspots and response to global change. Two major classes of methods have been employed to accomplish the large tree-building task: supertrees and supermatrices. Although these methods are continually being developed, they have yet to be made fully accessible to comparative biologists making extremely large trees rare.
  
  
  RESULTS
  Here we describe and demonstrate a modified supermatrix method termed mega-phylogeny that uses databased sequences as well as taxonomic hierarchies to make extremely large trees with denser matrices than supermatrices. The two major challenges facing large-scale supermatrix phylogenetics are assembling large data matrices from databases and reconstructing trees from those datasets. The mega-phylogeny approach addresses the former as the latter is accomplished by employing recently developed methods that have greatly reduced the run time of large phylogeny construction. We present an algorithm that requires relatively little human intervention. The implemented algorithm is demonstrated with a dataset and phylogeny for Asterales (within Campanulidae) containing 4954 species and 12,033 sites and an rbcL matrix for green plants (Viridiplantae) with 13,533 species and 1,401 sites.
  
  
  CONCLUSIONS
  By examining much larger phylogenies, patterns emerge that were otherwise unseen. The phylogeny of Viridiplantae successfully reconstructs major relationships of vascular plants that previously required many more genes. These demonstrations underscore the importance of using large phylogenies to uncover important evolutionary patterns and we present a fast and simple method for constructing these phylogenies.
authors.affiliation: National Evolutionary Synthesis Center, 2024 W Main St A200, Durham, NC 27705, USA. sasmith@nescent.org
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Smith
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Beaulieu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Donoghue
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis; D030541:Databases, Genetic; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D016013:Likelihood Functions; D010802:Phylogeny; D010944:Plants / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018744:DNA, Plant