title: Biocompatibility and osseointegration of reconstituted keratin in an ovine model.
abstract: Reconstituted keratin has potential as a raw material for orthopaedic applications. The aim of this study was to investigate the in vivo biocompatibility and osseointegration of keratin materials in an ovine model. Six different modifications of the keratin polymer, based on porous or dense constructs, regenerated by either neutral or acidic treatment, with or without hydroxyapatite, were made as small rods and inserted into drilled round defects in the femur and tibia of sheep. Histology was carried out on samples taken at different time points up to 24 weeks postsurgery. All keratin implants showed similar histological profiles, which included granulation tissue surrounding and infiltrating the implants, followed by new bone formation radiating from the existing bone. By 8 weeks, new bone had grown to within a short distance of the implant surface, and in some places was in direct apposition to the keratin implant. In the 12 to 24-week period, there was peripheral resorption and infiltration of bony trabeculae with regard to the porous constructs only. The tissue reaction appeared to model that of a fairly inert material. Further work on improving the extent of osseointegration and acceleration of the biodegradation rate of reconstituted keratin is underway.
authors.affiliation: Department of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand. george.dias@otago.ac.nz
authors.forename: George J
authors.initials: GJ
authors.lastname: Dias
authors.forename: Philip V
authors.initials: PV
authors.lastname: Peplow
authors.forename: Andrew
authors.initials: A
authors.lastname: McLaughlin
authors.forename: Fernanda
authors.initials: F
authors.lastname: Teixeira
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kelly
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000378:metabolism*; D017886:Durapatite / Q000737:chemistry; D005260:Female; D005269:Femur / Q000033:anatomy & histology / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D007633:Keratins / Q000378:metabolism* / Q000633:toxicity*; D008422:Materials Testing; D010316:Particle Size; D016062:Porosity; D012756:Sheep; D013977:Tibia / Q000033:anatomy & histology / Q000254:growth & development
chemical_list: D001672:Biocompatible Materials; D007633:Keratins; D017886:Durapatite