Characterization and regulation of basal calcium influx in human peripheral blood lymphocytes.
The basal 45Ca2+ influx into resting human blood lymphocytes was measured. This process showed biphasic kinetics with first rapid phase followed by the second long-lasting and markedly slower phase. Further, it showed signs of saturability and reaches maximal values at 37 degrees C and extracellular pH 7.2. The basal 45Ca2+ influx was stimulated by addition of submicromolar concentrations of 4 beta-phorbol-12-myristate-13-acetate, and this effect was abolished by protein kinase C (PKC) inhibitor Ro-31-8220. In the regulation of basal 45Ca2+ influx is probably only partially involved adenylate cyclase pathway as show results with intracellular c-AMP elevating agents (dB-c-AMP, 3-isobutyl-1-metylxantine and forskolin). Uncoupler 3,3',4',5-tetrachloro-salicylanilide (TCS) in micromolar concentrations stimulated basal 45Ca2+ influx and its effect was more significant in media with high extracellular concentration of K+.
Lakatos
Boris
B
Department of Biochemistry and Microbiology, Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia. boris.lakatos@stuba.sk
Slováková
Jana
J
Kaiserová
Karin
K
Orlický
Jozef
J
Varecka
L'udovít
L
D001693:Biological Transport, Active; D002118:Calcium / Q000502:physiology*; D002132:Calcium Radioisotopes; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D010703:Phorbol Esters / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012458:Salicylanilides / Q000494:pharmacology
D002132:Calcium Radioisotopes; D007211:Indoles; D010703:Phorbol Esters; D012458:Salicylanilides; D005576:Colforsin; D000242:Cyclic AMP; D011493:Protein Kinase C; C003390:3,3',4',5-tetrachlorosalicylanilide; D011188:Potassium; D002118:Calcium; C064758:Ro 31-8220