title: State-dependence of climbing fiber-driven calcium transients in Purkinje cells.
abstract: The recently described bi-stability of Purkinje cells and the state-dependence of the complex spike waveform suggest that calcium currents may play a pivotal role in both the complex spike waveform and the state of the membrane voltage. Here we used Ca2+ imaging to record the changes in intracellular [Ca2+] that are elicited by either spontaneous or climbing fiber-evoked activity in rat Purkinje cells. We show that a continuous somatic Ca2+ influx occurs during an "UP" state. Furthermore Ca2+ transients that are evoked by climbing fiber stimulation are state-dependent. Somatic transients are smaller following an "UP" state, while dendritic transients are smaller following a "DOWN" state. The state-dependence of these signals should affect the intrinsic firing of Purkinje cells as well as plastic processes that modulate synaptic strength.
authors.affiliation: Department of Neurobiology, Institute for Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. dan.rokni@mail.huji.ac.il
authors.forename: D
authors.initials: D
authors.lastname: Rokni
authors.forename: Y
authors.initials: Y
authors.lastname: Yarom
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology; D003712:Dendrites / Q000378:metabolism; D004558:Electric Stimulation / Q000379:methods; D066298:In Vitro Techniques; D009412:Nerve Fibers / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002118:Calcium