Integrated oxidative folding of cysteine/selenocysteine containing peptides: improving chemical synthesis of conotoxins.
Building bridges: The use of diselenide and selectively ((15)N/(13)C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy.
Walewska
Aleksandra
A
Department of Biology, University of Utah, Salt Lake City, Utah 84108, USA.
Zhang
Min-Min
MM
Skalicky
Jack J
JJ
Yoshikami
Doju
D
Olivera
Baldomero M
BM
Bulaj
Grzegorz
G
D020916:Conotoxins / Q000138:chemical synthesis* / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*; D017510:Protein Folding; D017279:Selenocysteine / Q000737:chemistry*
D020916:Conotoxins; D004220:Disulfides; D010455:Peptides; D017279:Selenocysteine; D003545:Cysteine