title: Individual architecture of subgingival microflora in chronic periodontitis.
abstract: BACKGROUND
  Nearly 400 species of bacteria have been found in human periodontal pockets, and half of them remain uncultured to date. The diagnosis of individual periodontal microflora using culture-independent detection of species is expected to serve as a tool for 'tailor-made' periodontal treatments. However, the richness and abundance of bacterial species within individual subgingival microflora have not been sufficiently studied to develop more specific diagnostic microbiological tests. The purpose of this study was to determine the ecological architecture of the subgingival microflora among chronic periodontitis patients and their individual differences based on phylotype analyses.
  
  
  METHODS
  Four 16S rRNA gene libraries were constructed from subgingival plaque samples taken from all diseased sites in four chronic periodontitis patients. The 480 clones generated from each of the four libraries were analyzed by phylotyping and subjected to ecological estimation of species richness.
  
  
  RESULTS
  The indices of species richness of the four libraries were 73, 75, 98 or 100 phylotypes. A total of 195 phylotypes were identified in the combined libraries. The majority of phylotypes were assigned to the Bacteroidetes, Fusobacteria, Proteobacteria, Firmicutes and Actinobacteria phyla. The phylotypes assigned to the Bacteroidetes and Fusobacteria phyla were the most predominant in each library (chi2-test, p < 0.05).
  
  
  CONCLUSIONS
  In this study, the molecular ecology of 1920 clones obtained from four patients' subgingival plaque samples was examined. More than half of the detected clones were categorized under either the Bacteroidetes or the Fusobacteria phyla. Each library showed unique richness and abundance of phylotypes.
authors.affiliation: Department of Periodontology, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Sasamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nagai
authors.forename: Ryuji
authors.initials: R
authors.lastname: Sakagami
authors.forename: Kenji
authors.initials: K
authors.lastname: Kitamura
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Miki
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D055113:Chronic Periodontitis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology*; D017753:Ecosystem; D005260:Female; D015723:Gene Library; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial