title: Mesenteric lymphatic ducts ligation decreases the degree of gut-induced lung injury in a portal vein occlusion and reperfusion canine model.
abstract: BACKGROUND
  Whether the mesenteric lymphatic system could serve as a route of transport by which gut-derived inflammatory mediators contribute to the induction of remote organ injuries is uncertain. We therefore made a gut-induced lung injury canine model by portal vein occlusion and reperfusion (PV O/R) and studied the role of mesenteric lymphatic ducts ligation (ML) to gut-induced lung injury with this model.
  
  
  METHODS
  Eighteen mongrel dogs were divided into control, PV O/R, and PV O/R + ML groups. Cytokines and endotoxin levels in the portal vein and lymph from thoracic duct in different groups were tested. The permeability, myeloperoxidase activity, and histopathological investigation of intestine and lung were evaluated.
  
  
  RESULTS
  Cytokines and endotoxin levels in the portal vein were significantly increased in experimental groups compared with control group (P < 0.05), and that in the lymph from thoracic duct were significantly increased in PV O/R group compared with control and PV O/R + ML group (P < 0.05). Lung permeability and MPO activity in PV O/R group were significantly higher than those in control and PV O/R + ML group (P < 0.05); intestinal permeability in experimental groups were significantly higher with respect to control group. The lung injury score in PV O/R group was significantly higher than those in control and PV O/R + ML group (P < 0.05) and the intestinal injury scores in experimental groups were significantly higher than control group (P < 0.05).
  
  
  CONCLUSIONS
  The gut-induced lung injury canine model made by PV O/R is successful, and mesenteric lymphatic ducts ligation decreases the degree of gut-induced lung injury in this model.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, School of Medicine, Xi'an Jiao Tong University, Xi'an, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Qifei
authors.initials: Q
authors.lastname: Wu
authors.forename: Quanyuan
authors.initials: Q
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Haibo
authors.initials: H
authors.lastname: Su
authors.forename: Naiying
authors.initials: N
authors.lastname: Shen
authors.forename: Minghui
authors.initials: M
authors.lastname: Tai
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D008026:Ligation / Q000379:methods / Q000662:veterinary; D055370:Lung Injury / Q000209:etiology* / Q000601:surgery; D008206:Lymphatic Diseases / Q000601:surgery*; D011169:Portal Vein / Q000601:surgery*; D015424:Reperfusion; D015427:Reperfusion Injury / Q000517:prevention & control*; D014652:Vascular Diseases / Q000601:surgery*