Fast genomic muChIP-chip from 1,000 cells.
Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (muChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. muChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.
Dahl
John Arne
JA
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway.
Reiner
Andrew H
AH
Collas
Philippe
P
D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008722:Methods; D046228:Microarray Analysis / Q000379:methods*; D011499:Protein Processing, Post-Translational
D006657:Histones