title: [Reconstruction and transplantation of the composite skin comprising epithelial growth factor gene-transfected keratinocytes].
abstract: OBJECTIVE
  To investigate the epithelial growth factor (EGF) expression of EGF gene-transfected keratinocytes and its effect on cell proliferation after grafting.
  
  
  METHODS
  Newborn Balb/c mouse keratinocytes and gene transfected keratinocytes were seeded on the surface of acellular dermal matrix and cocultured in different ratios as follows: 1:1, 1:3, or 1:5 1 week after culture. The composite skin was grafted onto the full-thickness wound in Balb/c mouse. Specimen was harvested at interval after grafting and underwent the immunohistochemistry staining for EGF and proliferating cell nuclear antigen (PCNA).
  
  
  RESULTS
  Immunohistochemical staining showed EGF was expressed in the newly generated epidermis 1-2 week after grafting of the composite skin comprising Balb/c mouse keratinocytes and gene-transfected keratinocytes (at the ratio of 1:5). One week after surgery, Anti-PCNA positive basal cells were more than that in composite skin containing Balb/c mouse keratinocytes alone (P<0.01).
  
  
  CONCLUSIONS
  The gene-transfected keratinocytes expresses EGF and promotes the proliferation of keratinocytes in the early stage after transplantation.
authors.affiliation: Department of Burn, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Shi-chu
authors.initials: SC
authors.lastname: Xiao
authors.forename: Shi-hui
authors.initials: SH
authors.lastname: Zhu
authors.forename: Zhao-fan
authors.initials: ZF
authors.lastname: Xia
authors.forename: Guang-qing
authors.initials: GQ
authors.lastname: Wang
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004815:Epidermal Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000293:injuries*; D016038:Skin Transplantation; D023822:Tissue Engineering; D014162:Transfection
chemical_list: D004815:Epidermal Growth Factor