Enhanced biodegradation of pentachlorophenol in unsaturated soil using reversed field electrokinetics.
This study investigated the use of electrokinetics in unsaturated soil to promote biodegradation of pentachlorophenol through increased contact between bacteria and contaminant. Soil microcosms, contaminated with approximately 100 mg kg(-1) pentachlorophenol (containing [(14)C]-PCP as a tracer), and inoculated with a specific pentachlorophenol-degrading bacterium (Sphingobium sp. UG30-1 x 10(8) cfu g(-1)) were subjected to constant and regularly reversed electric currents (10 mA). The former caused large pH and moisture content changes due to water electrolysis and electroosmotic effects, with subsequent negative impacts on biodegradation parameters including enzyme activity and contaminant mineralisation (as measured by (14)CO(2) evolution rate). The reversed field caused little change in pH and moisture content and led to more rapid contaminant mineralisation, lower soil contaminant concentration in the majority of the microcosms and increased soil enzyme activity (with the exception of soil immediately adjacent to the anode). The presence of an electric field, if suitably applied, may therefore enhance contaminant biodegradation in unsaturated soil.
Harbottle
Michael J
MJ
Department of Engineering Science, University of Oxford, Oxford, UK. harbottlem@cardiff.ac.uk
Lear
Gavin
G
Sills
Gilliane C
GC
Thompson
Ian P
IP
D001673:Biodegradation, Environmental; D004572:Electrolysis / Q000379:methods; D053841:Electroosmosis / Q000379:methods; D007700:Kinetics; D010416:Pentachlorophenol / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D045249:Sphingobacterium / Q000378:metabolism*
D012989:Soil Pollutants; D010416:Pentachlorophenol