title: Optimization of bioassay method for the quantitative microbiological determination of amphotericin B.
abstract: The aim of this study was to find the optimal bioassay parameters for the quantitative analysis of an amphotericin B nasal spray solution as the bioassay conditions recommended by the Ph. Eur. 6. were less sensitive and were only applicable for the measurement of a narrow concentration range, which makes the method unsuitable in case of a stability test. We evaluated five commonly used assay media with Candida albicans and Saccharomyces cerevisiae as test organisms. Our results showed that Mueller Hinton Agar supplemented with 2% glucose and 0.5 microg ml(-1) methylene blue inoculated with C. albicans gave the best bioassay circumstance as a wide concentration range (1.54-60.0 microg ml(-1) amphotericin B) could be measured and the inhibition zone borders were distinct and easy to read.
authors.affiliation: Department of Pharmaceutics and University Pharmacy, University of Pécs, Pécs, Hungary. andras.fittler@aok.pte.hu
authors.forename: András
authors.initials: A
authors.lastname: Fittler
authors.forename: Béla
authors.initials: B
authors.lastname: Kocsis
authors.forename: Imre
authors.initials: I
authors.lastname: Gerlinger
authors.forename: Lajos
authors.initials: L
authors.lastname: Botz
mesh_terms: D000666:Amphotericin B / Q000032:analysis*; D000935:Antifungal Agents / Q000032:analysis*; D001681:Biological Assay / Q000379:methods*; D002176:Candida albicans / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012680:Sensitivity and Specificity
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D000666:Amphotericin B