High mobility group box chromosomal protein 1 acts as a proliferation signal for activated T lymphocytes.
The nuclear protein high mobility group box chromosomal protein 1 (HMGB1) can be translocated extracellularly and plays a well-established role as a pro-inflammatory mediator during innate immune responses. Much less is known about the role of HMGB1 in adaptive immunity, since only a few studies have addressed the issue. We herein activated subsets of purified, primary human T lymphocytes with solid-phase bound anti-CD3 mAb and assessed the effects of recombinant HMGB1 protein on cell proliferation when added to the cultures. HMGB1 acted as a proliferative signal for human T cells during suboptimal anti-CD3 mAb stimulation. Statistically significant increased proliferation was recorded in both CD4+ and CD8+ T-cell cultures at HMGB1 concentrations ranging from 0.25 to 1.0 microg/ml. HMGB1 had no effect on proliferation in the absence of anti-CD3 stimulation or during T-cell activation obtained using high doses of anti-CD3 mAb. Our results demonstrate a direct HMGB1-mediated effect in adaptive immunity.
Sundberg
Erik
E
Department of Woman and Child Health, Pediatric Rheumatology Research Unit, Karolinska Institutet/Karolinska University Hospital, L 8:04 CMM, S-17176 Stockholm, Sweden. Erik.Sundberg@ki.se
Fasth
Andreas E R
AE
Palmblad
Karin
K
Harris
Helena Erlandsson
HE
Andersson
Ulf
U
D000911:Antibodies, Monoclonal; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry; D024243:HMGB1 Protein / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D024243:HMGB1 Protein; D011994:Recombinant Proteins