Effect of physical conditions and chemicals on the binding of a mini-CbpA from Clostridium cellulovorans to a semi-crystalline cellulose ligand.
OBJECTIVE
To investigate the effect that environmental factors have on Clostridium cellulovorans cellulose binding domain (CBD) binding to a semi-crystalline cellulose ligand, namely Avicel.


RESULTS
The behaviour of a 58 kDa mini-CbpA protein containing the CBD from the scaffoldin protein of C. cellulovorans was studied in the presence of various environmental factors, in order to determine whether such factors promote or reduce CBD binding to its ligand, thus potentially affecting its activity on the substrate. The amount of binding was found to be dependent on the Avicel concentration and optimal binding occurred when the ligand concentration was 15 mg ml(-1). Optimal CBD binding occurred at pH 7.0 and at an incubation temperature of 28 degrees C. The effects of dithiothreitol (DTT), 2-mercaptoethanol, acetone, butanol, ethanol and butyric acid were also investigated.


CONCLUSIONS
Temperature, pH, DTT, 2-mercaptoethanol and solvents were shown to affect the binding of C. cellulovorans CBD to Avicel.


CONCLUSIONS
Clostridium cellulovorans CBD binding to Avicel is affected by physical conditions and chemicals.
Lavan
L M
LM
Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown, South Africa.
Van Dyk
J S
JS
Chan
H
H
Doi
R H
RH
Pletschke
B I
BI
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D046974:Clostridium cellulovorans / Q000201:enzymology / Q000378:metabolism*; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008623:Mercaptoethanol; D012997:Solvents; D013696:Temperature
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008024:Ligands; D012997:Solvents; C074074:cellulose binding protein A, Clostridium cellulovorans; D008623:Mercaptoethanol; D002482:Cellulose