title: PDLIM4, an actin binding protein, suppresses prostate cancer cell growth.
abstract: We investigated the molecular function of PDLIM4 in prostate cancer cells. PDLIM4 mRNA and protein-expression levels were reduced in LNCaP, LAPC4, DU145, CWR22, and PC3 prostate cancer cells. The re-expression of PDLIM4 in prostate cancer cells has significantly reduced the cell growth and clonogenicity with G1 phase of cell-cycle arrest. We have shown the direct interaction of PDLIM4 with F-actin. Restoration of PDLIM4 expression resulted in reduction of tumor growth in xenografts. These results suggest that PDLIM4 may function as a tumor suppressor, involved in the control of cell proliferation by associating with actin in prostate cancer cells.
authors.affiliation: Department of Urology, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Donkena Krishna
authors.initials: DK
authors.lastname: Vanaja
authors.forename: Michael E
authors.initials: ME
authors.lastname: Grossmann
authors.forename: John C
authors.initials: JC
authors.lastname: Cheville
authors.forename: Mozammel H
authors.initials: MH
authors.lastname: Gazi
authors.forename: Aiyu
authors.initials: A
authors.lastname: Gong
authors.forename: Jin San
authors.initials: JS
authors.lastname: Zhang
authors.forename: Katalin
authors.initials: K
authors.lastname: Ajtai
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Burghardt
authors.forename: Charles Y F
authors.initials: CY
authors.lastname: Young
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D060588:LIM Domain Proteins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins; D011471:Prostatic Neoplasms / Q000473:pathology / Q000517:prevention & control*; D025521:Tumor Suppressor Proteins / Q000502:physiology*
chemical_list: D000199:Actins; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; D008840:Microfilament Proteins; C486247:PDLIM4 protein, human; C486248:Pdlim4 protein, mouse; D025521:Tumor Suppressor Proteins