title: Multiple RNAs from the mouse carboxypeptidase M locus: functional RNAs or transcription noise?
abstract: BACKGROUND
  A major effort of the scientific community has been to obtain complete pictures of the genomes of many organisms. This has been accomplished mainly by annotation of structural and functional elements in the genome sequence, a process that has been centred in the gene concept and, as a consequence, biased toward protein coding sequences. Recently, the explosion of transcriptome data generated and the discovery of many functional non-protein coding RNAs have painted a more detailed and complex scenario for the genome. Here we analyzed the mouse carboxypeptidase M locus in this broader perspective in order to define the mouse CPM gene structure and evaluate the existence of other transcripts from the same genomic region.
  
  
  RESULTS
  Bioinformatic analysis of nucleotide sequences that map to the mouse CPM locus suggests that, in addition to the mouse CPM mRNA, it expresses at least 33 different transcripts, many of which seem to be non-coding RNAs. We randomly chose to evaluate experimentally four of these extra transcripts. They are expressed in a tissue specific manner, indicating that they are not artefacts or transcriptional noise. Furthermore, one of these four extra transcripts shows expression patterns that differed considerably from the other ones and from the mouse CPM gene, suggesting that there may be more than one transcriptional unit in this locus. In addition, we have confirmed the mouse CPM gene RefSeq sequence by rapid amplification of cDNA ends (RACE) and directional cloning.
  
  
  CONCLUSIONS
  This study supports the recent view that the majority of the genome is transcribed and that many of the resulting transcripts seem to be non-coding RNAs from introns of genes or from independent transcriptional units. Although some of the information on the transcriptome of many organisms may actually be artefacts or transcriptional noise, we argue that it can be experimentally evaluated and used to find and define biological functional elements on the genome. Furthermore, the transcription of other functional RNAs besides the protein coding RNA from a specific genomic locus imposes extra care when designing and interpreting experiments involving genetic manipulations or expression detection and quantification.
authors.affiliation: Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil. sander@biofis.epm.br
authors.forename: Alessander O
authors.initials: AO
authors.lastname: Guimarães
authors.forename: Fabiana L
authors.initials: FL
authors.lastname: Motta
authors.forename: Viviane S
authors.initials: VS
authors.lastname: Alves
authors.forename: Beatriz A
authors.initials: BA
authors.lastname: Castilho
authors.forename: João B
authors.initials: JB
authors.lastname: Pesquero
mesh_terms: D000818:Animals; D016477:Artifacts; D001483:Base Sequence; D019295:Computational Biology; D005260:Female; D058851:GPI-Linked Proteins; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D023061:Gene Order; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D039104:RNA 3' Polyadenylation Signals; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D058851:GPI-Linked Proteins; D012313:RNA; C058479:carboxypeptidase M; D008666:Metalloendopeptidases