title: Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP.
abstract: Interleukin-22 (IL-22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL-22 was incubated with its soluble decoy receptor IL-22BP (IL-22 binding protein) and the IL-22-IL-22BP complex was crystallized in hanging drops using the vapour-diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 A resolution. The crystal belonged to the tetragonal space group P4(1), with unit-cell parameters a = b = 67.9, c = 172.5 A, and contained two IL-22-IL-22BP complexes per asymmetric unit.
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São-Carlense 400, 13560-970 São Carlos-SP, Brazil.
authors.forename: Leandra
authors.initials: L
authors.lastname: Watanabe
authors.forename: Patricia Ribeiro
authors.initials: PR
authors.lastname: de Moura
authors.forename: Alessandro Silva
authors.initials: AS
authors.lastname: Nascimento
authors.forename: Didier
authors.initials: D
authors.lastname: Colau
authors.forename: Laure
authors.initials: L
authors.lastname: Dumoutier
authors.forename: Jean Christophe
authors.initials: JC
authors.lastname: Renauld
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D003460:Crystallization; D006801:Humans; D007378:Interleukins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D018123:Receptors, Interleukin / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D014961:X-Ray Diffraction / Q000379:methods; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D000097043:Interleukin-22; C433253:IL22RA2 protein, human