Comparison of high-performance liquid chromatographic and thin-layer chromatographic methods for determination of aloin in herbal products containing Aloe vera.
Aloe vera is a medicinal plant used worldwide to treat a variety of conditions and, as such, has important commercial value. Aloin is a principal component of aloe vera leaves and is used for quality control of products containing it. A semiquantitative thin-layer chromatographic (TLC) method for determining the concentration of aloin in aloe-based products was validated. The results were similar to those of a validated high-performance liquid chromatographic method; therefore, TLC, which is a simple, sensitive, specific, rapid, and cheap method, may be ideal for use in any laboratory for routine analysis of commercial products containing aloe vera.
Ramírez Durón
Rosalba
R
Universidad Autónoma de Nuevo León, Departamento de Química Analitica, Facultad de Medicina, PO Box 2316, Sucursal Tecnológico, 64841 Monterrey Nuevo León, Mexico.
Ceniceros Almaguer
Lucía
L
Cavazos Rocha
Norma Cecilia
NC
Silva Flores
Perla Giovanna
PG
De Torres
Noemí Waksman
NW
D000504:Aloe / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004642:Emodin / Q000031:analogs & derivatives* / Q000032:analysis; D007202:Indicators and Reagents; D010936:Plant Extracts / Q000032:analysis; D028321:Plant Preparations / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents
D007202:Indicators and Reagents; D010936:Plant Extracts; D028321:Plant Preparations; D012997:Solvents; D004642:Emodin; C006457:alloin