Cloning and immunoreactivity of the 5-HT 1Mac and 5-HT 2Mac receptors in the central nervous system of the freshwater prawn Macrobrachium rosenbergii.
Biogenic amines are implicated in several mental disorders, many of which involve social interactions. Simple model systems, such as crustaceans, are often more amenable than vertebrates for studying mechanisms underlying behaviors. Although various cellular responses of biogenic amines have been characterized in crustaceans, the mechanisms linking these molecules to behavior remain largely unknown. Observed effects of serotonin receptor agonists and antagonists in abdomen posture, escape responses, and fighting have led to the suggestion that biogenic amine receptors may play a role in modulating interactive behaviors. As a first step in understanding this potential role of such receptors, we have cloned and fully sequenced two serotonin receptors, 5-HT(1Mac) and 5-HT(2Mac), from the CNS of the freshwater prawn Macrobrachium rosenbergii and have mapped their CNS immunohistochemical distribution. 5-HT(1Mac) was found primarily on the membranes of subsets of cells in all CNS ganglia, in fibers that traverse all CNS regions, and in the cytoplasm of a small number of cells in the brain and circum- and subesophageal ganglia (SEG), most of which also appear to contain dopamine. The pattern of 5-HT(2Mac) immunoreactivity was found to differ significantly; it was found mostly in the central neuropil area of all ganglia, in glomeruli of the brain's olfactory lobes, and in the cytoplasm of a small number of neurons in the SEG, thoracic, and some abdominal ganglia. The observed differences in terms of localization, distribution within cells, and intensity of immunoreactive staining throughout the prawn's CNS suggest that these receptors are likely to play different roles.
Vázquez-Acevedo
Nietzell
N
Department of Anatomy and Neurobiology, School of Medicine, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico 00936, USA.
Reyes-Colón
Dalynés
D
Ruíz-Rodríguez
Eduardo A
EA
Rivera
Nilsa M
NM
Rosenthal
Joshua
J
Kohn
Andrea B
AB
Moroz
Leonid L
LL
Sosa
María A
MA
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002490:Central Nervous System / Q000378:metabolism; D017124:Conserved Sequence; D004298:Dopamine / Q000378:metabolism; D019143:Evolution, Molecular; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D044263:Receptors, Serotonin, 5-HT1 / Q000235:genetics* / Q000378:metabolism*; D044348:Receptors, Serotonin, 5-HT2 / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D000906:Antibodies; D044263:Receptors, Serotonin, 5-HT1; D044348:Receptors, Serotonin, 5-HT2; D004298:Dopamine