In vivo off-resonance saturation magnetic resonance imaging of alphavbeta3-targeted superparamagnetic nanoparticles.
Magnetic resonance imaging is a powerful clinical imaging technique that allows for noninvasive tomographic visualization of anatomic structures with high spatial resolution and soft tissue contrast. However, its application in molecular imaging of cancer has been limited by the lack of sensitivity and detection accuracy in depicting the biochemical expression of these diseases. Here, we combine an ultrasensitive design of superparamagnetic polymeric micelles (SPPM) and an off-resonance saturation (ORS) method to enhance the imaging efficacy of tumor biomarkers in vivo. SPPM nanoparticles encoded with cyclic(RGDfK) were able to target the alpha(v)beta(3)-expressing microvasculature in A549 non-small cell lung tumor xenografts in mice. ORS greatly improved tumor detection accuracy over the conventional T(2)*-weighted method by its ability to turn "ON" the contrast of SPPM. This combination of ORS imaging with a tumor vasculature-targeted, ultrasensitive SPPM design offers new opportunities in molecular imaging of cancer.
Khemtong
Chalermchai
C
Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas 35790, USA.
Kessinger
Chase W
CW
Ren
Jimin
J
Bey
Erik A
EA
Yang
Su-Geun
SG
Guthi
Jagadeesh Setti
JS
Boothman
David A
DA
Sherry
A Dean
AD
Gao
Jinming
J
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002196:Capillaries / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply / Q000473:pathology*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000032:analysis* / Q000378:metabolism; D008175:Lung Neoplasms / Q000098:blood supply / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D008833:Microcirculation; D053758:Nanoparticles; D019047:Phantoms, Imaging; D011971:Receptors, Immunologic / Q000032:analysis; D018000:Receptors, Peptide / Q000032:analysis; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous; D014316:Tritium
D014408:Biomarkers, Tumor; D039302:Integrin alphaVbeta3; D008823:Micelles; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C051382:arginyl-glycyl-aspartic acid directed cell adhesion receptor; D014316:Tritium