Immunocytochemical Localization of Glucagon-Related Peptides in the Cerebral Ganglion of the Protochordate Ascidian, Styela plicata.
Abstract The central nervous system of the protochordate ascidian Styela plicata has been investigated by means of immunocytochemical techniques in order to localize peptides of the glucagon/secretin family. In particular, glucagon- and peptide histidine isoleucine-containing neurons were localized in the cortex of the cerebral ganglion, and numerous nerve cell bodies containing vasoactive intestinal polypeptide occurred mainly in the medullary zone. Moreover, coexistence of glucagon/peptide histidine isoleucine and glucagon/vasoactive intestinal polypeptide was detected in a few cortical neurons. The localization of peptide histidine isoleucine-like peptides in the central nervous system and alimentary tract supports the occurrence of a 'brain-gut axis' in protochordates as well as in vertebrates and some invertebrates, while glucagon- and vasoactive intestinal polypeptide-like substances can be considered exclusively as neuropeptides.
Pestarino
M
M
Istituto di Anatomia Comparata, Università di Genova, Viale Benedetto XV, 5, 16132 Genova, Italy.