title: Transglutaminase-dependent antiproliferative and differentiative properties of nimesulide on B16-F10 mouse melanoma cells.
abstract: The aim of this study was to collect evidences on the role of transglutaminase (TG, E.C.2.3.2.13) in the antineoplastic properties exerted by nimesulide (NMS), a non-steroidal anti-inflammatory drug, on murine B16-F10 melanoma cells. Treatment of melanoma cells with nimesulide produces a considerable reduction of cell proliferation, paralleled by a remarkable decrease of the intracellular concentration of polyamines spermidine and spermine. NMS treatment induces cancer cell differentiation, likely through the observed enhancement of TG and tyrosinase activities and increase of melanin production, well known markers of melanocyte differentiation. The overall results highlight the possibility that nimesulide acts as antineoplastic agent likely through the induction of intracellular TG activity.
authors.affiliation: Department of Biology, University Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Gismondi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lentini
authors.forename: Claudio
authors.initials: C
authors.lastname: Tabolacci
authors.forename: Bruno
authors.initials: B
authors.lastname: Provenzano
authors.forename: Simone
authors.initials: S
authors.lastname: Beninati
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004789:Enzyme Activation; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000188:drug therapy / Q000201:enzymology* / Q000378:metabolism / Q000503:physiopathology; D008546:Melanoma, Experimental; D051379:Mice; D011073:Polyamines / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology*; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D008543:Melanins; D011073:Polyamines; D013449:Sulfonamides; D011503:Transglutaminases; C012655:nimesulide