Sensing of UO22+ and design of logic gates by the application of supramolecular constructs of ion-dependent DNAzymes.
Supramolecular constructs composed of ion-dependent DNAzymes and their substrates were used to develop DNAzyme cascades that enabled the sensitive detection of UO22+ or the activation of logic gate operations. The supramolecular complex between the UO22+-dependent DNAzyme and its substrate leads, in the presence of UO22+, to the cleavage of the substrate and to the release of the HRP-mimicking DNAzyme that enables the optical analysis of UO22+ (detection limit 1 x 10-9 M). Similarly, supramolecular complexes between the Mg2+- and UO22+-dependent DNAzymes and tailored substrates enables the design of the "OR" and "AND" logic gates, using Mg2+ and UO22+ as inputs.
Moshe
Michal
M
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Elbaz
Johann
J
Willner
Itamar
I
D001672:Biocompatible Materials; D015374:Biosensing Techniques; D002384:Catalysis; D039301:Computers, Molecular; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D021881:DNA, Catalytic / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D008956:Models, Chemical; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation; D013997:Time Factors
D001672:Biocompatible Materials; D021881:DNA, Catalytic; D004247:DNA; D008274:Magnesium