title: Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two monoclonal antibodies.
abstract: An immunoaffinity chromatographic method was developed for the purification of 9 sulfonamides (sulfamethazine, sulfadimethoxine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamonomethoxine, and sulfapyridine) from chicken tissue (muscle and liver) samples. Two monoclonal antibodies (antisulfamethazine and antisulfamethoxazole) were simultaneously covalently coupled to CNBr-activated Sepharose 4B for the preparation of a re-usable immunoaffinity column. After extraction with methanol-water and purification by immunoaffinity column, the sulfonamides were determined by reversed-phase liquid chromatography and UV detection at 270 nm. The recoveries for each drug at fortification levels of 10-50 ng/g ranged from 74.1 to 108.9% with relative standard deviations from 1.9 to 11.5%. The limits of detection were 2 ng/g for each drug.
authors.affiliation: China Agricultural University, National Reference Laboratory for Veterinary Drug Residue, Department of Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100094, People's Republic of China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Li
authors.forename: Cun
authors.initials: C
authors.lastname: Li
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.forename: Zhahnui
authors.initials: Z
authors.lastname: Wang
authors.forename: Xingyuan
authors.initials: X
authors.lastname: Cao
authors.forename: Sijun
authors.initials: S
authors.lastname: Zhao
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002138:Calibration; D002645:Chickens / Q000378:metabolism*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D012015:Reference Standards; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D012996:Solutions; D013449:Sulfonamides