title: Norsesquiterpene hydrocarbon, chemical composition and antimicrobial activity of Rhaponticum carthamoides root essential oil.
abstract: A detailed analysis of Rhaponticum carthamoides (Willd.) Iljin root essential oil was carried out by GC, GC-MS and GC-FTIR techniques. In total, 30 components were identified, accounting for 98.0% of total volatiles. A norsesquiterpene 13-norcypera-1(5),11(12)-diene (22.6%), followed by aplotaxene (21.2%) and cyperene (17.9%), were isolated and their structures confirmed by 1D and 2D-NMR spectra (COSY, ROESY, HSQC, HMBC and INADEQUATE). Selinene type sesquiterpenes and aliphatic hydrocarbons were among minor constituents of the essential oil. The oil exhibited antimicrobial activity against 5 of 9 strains of bacteria and yeast, when tested using broth micro-dilution method. Minimum inhibitory concentrations ranged between 32 and 256 microg/ml.
authors.affiliation: Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Kamycka 129, 165 21 Prague 6 - Suchdol, Czech Republic. havlik@af.czu.cz
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Havlik
authors.forename: Milos
authors.initials: M
authors.lastname: Budesinsky
authors.forename: Pavel
authors.initials: P
authors.lastname: Kloucek
authors.forename: Ladislav
authors.initials: L
authors.lastname: Kokoska
authors.forename: Irena
authors.initials: I
authors.lastname: Valterova
authors.forename: Sona
authors.initials: S
authors.lastname: Vasickova
authors.forename: Vaclav
authors.initials: V
authors.lastname: Zeleny
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D043843:Leuzea / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D015003:Yeasts / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile; D010938:Plant Oils