title: Influence of dietary fat on oxidative stress and inflammation in murine macrophages.
abstract: OBJECTIVE
  Many studies have shown that the nature of the lipid consumed in the diet significantly affects the development of inflammatory diseases. In this study, we compared the effect of diets supplemented with 15% by weight of fish oil (FO), refined olive oil (ROO), and pomace olive oil (POO) with that of a low-fat diet, 2% by weight of corn oil, considered as the basal diet (BD), on the ability to modify reactive oxidative species and proinflammatory mediator generation by stimulated murine macrophages.
  
  
  METHODS
  Mice were fed the different oil-enriched diets for 8 wk. Peritoneal macrophages were isolated from these mice and subsequently stimulated. Reactive oxygen species and proinflammatory mediators were measured in the corresponding supernatants. Data were statistically treated by one-way analysis of variance and Tukey's multiple comparison post hoc test.
  
  
  RESULTS
  The ROO and POO significantly reduced the hydrogen peroxide production compared with BD, whereas FO stimulated its production. Moreover, the generation of nitric oxide was significantly prevented in all the experimental oil-enriched dietary groups. The ROO and FO groups showed significantly reduced cytokine (tumor necrosis factor-alpha, interleukin-1beta, interleukin-6) and prostaglandin E(2) production.
  
  
  CONCLUSIONS
  These results confirm the prevention action on proinflammatory mediator generation exerted by FO and demonstrate the protective antioxidant properties not only of olive oil but also of POO. The consumption of these olive oils may help to prevent cellular oxidative stress and inflammation.
authors.affiliation: Pharmacology Department, Faculty of Pharmacy, University of Seville, Seville, Spain. puerta@us.es
authors.forename: Rocío
authors.initials: R
authors.lastname: de la Puerta
authors.forename: Ana
authors.initials: A
authors.lastname: Marquez-Martin
authors.forename: Angeles
authors.initials: A
authors.lastname: Fernandez-Arche
authors.forename: Valentina
authors.initials: V
authors.lastname: Ruiz-Gutierrez
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016207:Cytokines; D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D018836:Inflammation Mediators; D000069463:Olive Oil; D010938:Plant Oils; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D006861:Hydrogen Peroxide