[Expression and significance of vascular cell adhesion molecule-1 in human oral squamous cell carcinoma].
OBJECTIVE
To study the expression and the location of vascular cell adhesion molecule-1 (VCAM-1) gene and its clinical significance in human oral squamous cell carcinoma (OSCC).


METHODS
In situ hybridization, PV-9000 polymer detection system for immunohistochemical staining was used to detect the expression and the location of VCAM-1 mRNA and VCAM-1 protein in 48 cases of OSCC and 10 cases of normal controls. Statistical analysis was performed using chi-square test in SPSS 13.0.


RESULTS
VCAM-1 protein was mainly expressed in tumor cell cytoplasm and membrane, VCAM-1 mRNA was mainly expressed in tumor cell cytoplasm. The expression rate of VCAM-1 mRNA and VCAM-1 protein was significantly higher in OSCC than that in normal oral mucosa (P<0.01). The expression of VCAM-1 mRNA was positively correlated with that of VCAM-1 protein (P<0.01). In the clinicopathologic factors, lymph node metastasis and depth of infiltration were closely correlated with VCAM-1 expression (P<0.01). The expression of VCAM-1 was significantly higher in tumor with lymph node metastasis than in tumor without lymph node metastasis (P<0.01).


CONCLUSIONS
Overexpression of VCAM-1 gene in OSCC may play a potential role in the development of OSCC. The overexpression of VCAM-1 gene in OSCC may be related to the tumor infiltration and metastasis.
Sun
Le-gang
LG
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
Wang
Fang
F
Liu
Ling
L
Song
Yu-feng
YF
Yang
You-cheng
YC
Wang
Li-fang
LF
D002294:Carcinoma, Squamous Cell; D006801:Humans; D017403:In Situ Hybridization; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009061:Mouth Mucosa; D009062:Mouth Neoplasms; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1
D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1