Highly efficient and low-cost purification of lysozyme: a novel tris(hydroxymethyl)aminomethane immobilized affinity column.
A highly efficient and low-cost affinity chromatography strategy for lysozyme (LZM) purification is reported. Using tris(hydroxymethyl)aminomethane (Tris) as ligand and macroporous silica spheres as matrix, a novel affinity column was prepared. The high specificity, stability and repeatability of this Tris immobilized affinity column were proved by LZM separations from protein mixture solutions for 20 circles and 6 months test. LZM purified from chicken egg white on the Tris affinity column had even higher purity than the commercial standard and well-maintained activity of 8287 U/mg (activity of commercial LZM was 8171 U/mg). The efficient affinity process avoiding expensive or fragile ligand would bring advantages to the routine production of LZM from chicken egg white.
Quan
Li
L
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China.
Cao
Qing
Q
Li
Zhiyu
Z
Li
Na
N
Li
Kean
K
Liu
Feng
F
D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity / Q000191:economics* / Q000379:methods*; D004529:Egg White / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014325:Tromethamine / Q000737:chemistry*
D014325:Tromethamine; D009113:Muramidase