title: Single-point mutations causing more than 100-fold underestimation of human immunodeficiency virus type 1 (HIV-1) load with the Cobas TaqMan HIV-1 real-time PCR assay.
abstract: Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.
authors.affiliation: Institute of Clinical and Molecular Virology, National Reference Center for Retroviruses, University of Erlangen-Nuremberg, Erlangen, Germany. klaus.korn@viro.med.uni-erlangen.de
authors.forename: Klaus
authors.initials: K
authors.lastname: Korn
authors.forename: Benedikt
authors.initials: B
authors.lastname: Weissbrich
authors.forename: Cornelia
authors.initials: C
authors.lastname: Henke-Gendo
authors.forename: Albert
authors.initials: A
authors.lastname: Heim
authors.forename: Christin Mariel
authors.initials: CM
authors.lastname: Jauer
authors.forename: Ninon
authors.initials: N
authors.lastname: Taylor
authors.forename: Josef
authors.initials: J
authors.lastname: Eberle
mesh_terms: D001483:Base Sequence; D003951:Diagnostic Errors; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019562:Viral Load / Q000379:methods*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus