Neural stem cell- and Schwann cell-loaded biodegradable polymer scaffolds support axonal regeneration in the transected spinal cord.
Biodegradable polymer scaffolds provide an excellent approach to quantifying critical factors necessary for restoration of function after a transection spinal cord injury. Neural stem cells (NSCs) and Schwann cells (SCs) support axonal regeneration. This study examines the compatibility of NSCs and SCs with the poly-lactic-co-glycolic acid polymer scaffold and quantitatively assesses their potential to promote regeneration after a spinal cord transection injury in rats. NSCs were cultured as neurospheres and characterized by immunostaining for nestin (NSCs), glial fibrillary acidic protein (GFAP) (astrocytes), betaIII-tubulin (immature neurons), oligodendrocyte-4 (immature oligodendrocytes), and myelin oligodendrocyte (mature oligodendrocytes), while SCs were characterized by immunostaining for S-100. Rats with transection injuries received scaffold implants containing NSCs (n=17), SCs (n=17), and no cells (control) (n=8). The degree of axonal regeneration was determined by counting neurofilament-stained axons through the scaffold channels 1 month after transplantation. Serial sectioning through the scaffold channels in NSC- and SC-treated groups revealed the presence of nestin, neurofilament, S-100, and betaIII tubulin-positive cells. GFAP-positive cells were only seen at the spinal cord-scaffold border. There were significantly more axons in the NSC- and SC- treated groups compared to the control group. In conclusion, biodegradable scaffolds with aligned columns seeded with NSCs or SCs facilitate regeneration across the transected spinal cord. Further, these multichannel biodegradable polymer scaffolds effectively serve as platforms for quantitative analysis of axonal regeneration.
Olson
Heather E
HE
Department of Neurology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Rooney
Gemma E
GE
Gross
LouAnn
L
Nesbitt
Jarred J
JJ
Galvin
Katherine E
KE
Knight
Andrew
A
Chen
BingKun
B
Yaszemski
Michael J
MJ
Windebank
Anthony J
AJ
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D001672:Biocompatible Materials / Q000378:metabolism*; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D019344:Lactic Acid / Q000378:metabolism*; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology*; D011100:Polyglycolic Acid / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D016019:Survival Analysis; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid