title: [Relationship of myeloid differentiation-2 gene promoter polymorphisms with susceptivity of complications after severe trauma in Chinese Han population].
abstract: OBJECTIVE
  To investigate the polymorphisms of myeloid differentiation-2 (MD-2) gene promoters, and to explore whether such polymorphisms are associated with the susceptibility to multiple organ dysfunction syndrome (MODS) and sepsis in Chinese Han population.
  
  
  METHODS
  Using polymerase chain reaction-restriction fragment length polymorphism method, the authors detected the single nucleotide polymorphisms of the promoter region of MD-2 gene at position - 1625C/G in 105 severe trauma patients (42 with sepsis). The organ function was scored.
  
  
  RESULTS
  The frequency of CC genotype in MD-2 gene promoter region at position - 1625 was 0.5 (21/42) in septic patients and 0.7 (44/63) in non-septic patients. The frequency of CG genotype was 0.38 (16/42) in septic patients and 0.27 (17/63) in non-septic patients. The frequency of GG genotype was 0.12 (5/42) in septic patients and 0.03 (2/63) in non-septic patients. The MODS scores in trauma patients carrying G allele at position - 1625 were significantly higher than those carrying C allele (P<0.001 for dominant effect, and P>0.05 for recessive effect). Moreover, trauma patients carrying G allele appeared to have higher risk of sepsis comparing to those carrying C allele (OR 0.477, 95% CI 0.266-0.855, P<0.05). Sepsis morbidity was significantly different between subjects with C and G alleles (P<0.05 for dominant effect, P>0.05 for recessive effect).
  
  
  CONCLUSIONS
  The polymorphisms of the promoter region of MD-2 gene at position - 1625 C/G is correlated with MODS and sepsis after severe trauma in Chinese Han population. The people with - 1625 G allele in the promoter region of MD-2 gene may be a risk factor of severe complications.
authors.affiliation: Department Four of State Key Laboratory of Trauma, Bums and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: You-an
authors.initials: YA
authors.lastname: Shan
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Dong-po
authors.initials: DP
authors.lastname: Jiang
authors.forename: Yuan-zhang
authors.initials: YZ
authors.lastname: Yao
authors.forename: Lian-yang
authors.initials: LY
authors.lastname: Zhang
authors.forename: Ding-yuan
authors.initials: DY
authors.lastname: Du
authors.forename: Jin-mou
authors.initials: JM
authors.lastname: Gao
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.forename: Ce
authors.initials: C
authors.lastname: Yang
authors.forename: Pei-fang
authors.initials: PF
authors.lastname: Zhu
authors.forename: Zheng-guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Jiang
mesh_terms: D044466:Asian People; D002681:China; D020022:Genetic Predisposition to Disease; D006801:Humans; D051218:Lymphocyte Antigen 96 / Q000235:genetics*; D009102:Multiple Organ Failure / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D018805:Sepsis / Q000209:etiology / Q000235:genetics*; D014947:Wounds and Injuries / Q000150:complications / Q000235:genetics*
chemical_list: C495377:LY96 protein, human; D051218:Lymphocyte Antigen 96