Interleukin 10 (IL-10) gene variants and susceptibility for paediatric onset Crohn's disease.
BACKGROUND
A recent genome-wide association study in adult patients with ulcerative colitis (UC) has implicated the interleukin 10 (IL-10) gene as an important candidate gene. Moreover, a UC-associated single nucleotide polymorphism (SNP) rs3024405 was also significantly associated with adult Crohn's disease (CD).


OBJECTIVE
To examine whether IL-10-CD associations extended to paediatric-onset CD.


METHODS
We implemented the case-control design at three paediatric gastroenterology clinics in Canada. CD patients (<or=20 years) were recruited along with healthy controls. DNA samples were genotyped for tag-single nucleotide polymorphisms (tag-SNPs) in the IL-10 gene. Allelic, genotype and haplotype associations with CD were studied.


RESULTS
A total of 270 patients and 336 controls were studied. The mean age (+/-s.d.) at diagnosis was 12.1 (+/-3.5). There were a slightly higher proportion of male patients (56.3%). Of the five IL-10 tag-SNPs, rs2222202 (C/T) (P = 0.03) and rs1800871 (C/T) (P = 0.05) showed significant allelic associations with CD. Specific IL-10 SNPs were associated with CD disease location and/or disease behaviour.


CONCLUSIONS
Our gene-wide analysis replicates recent findings of associations between IL-10 and adult CD, and suggests that these associations extend to paediatric-onset CD as well.
Amre
D K
DK
Department of Pediatrics, University of Montreal, Montreal, QC, Canada. devendra.amre@umontreal.ca
Mack
D R
DR
Morgan
K
K
Israel
D
D
Lambrette
P
P
Costea
I
I
Krupoves
A
A
Fegury
H
H
Dong
J
J
Grimard
G
G
Deslandres
C
C
Levy
E
E
Seidman
E G
EG
D000293:Adolescent; D017668:Age of Onset; D002170:Canada / Q000453:epidemiology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003424:Crohn Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D012307:Risk Factors; D055815:Young Adult
D016753:Interleukin-10