Bacteroides induce higher IgA production than Lactobacillus by increasing activation-induced cytidine deaminase expression in B cells in murine Peyer's patches.
The gut mucosal immune system is crucial in host defense against infection by pathogenic microbacteria and viruses via the production of IgA. Previous studies have shown that intestinal commensal bacteria enhance mucosal IgA production. However, it is poorly understood how these bacteria induce IgA production and which genera of intestinal commensal bacteria induce IgA production effectively. In this study, we compared the immunomodulatory effects of Bacteroides and Lactobacillus on IgA production by Peyer's patches lymphocytes. IgA production by Peyer's patches lymphocytes co-cultured with Bacteroides was higher than with Lactobacillus. In addition, the expression of activation-induced cytidine deaminase increased in co-culture with Bacteroides but not with Lactobacillus. We found that intestinal commensal bacteria elicited IgA production. In particular, Bacteroides induced the differentiation of Peyer's patches B cell into IgA(+) B cells by increasing activation-induced cytidine deaminase expression.
Yanagibashi
Tsutomu
T
Department of Food Science and Technology, Nihon University, Fujisawa-shi, Kanagawa, Japan.
Hosono
Akira
A
Oyama
Akihito
A
Tsuda
Masato
M
Hachimura
Satoshi
S
Takahashi
Yoshimasa
Y
Itoh
Kikuji
K
Hirayama
Kazuhiro
K
Takahashi
Kyoko
K
Kaminogawa
Shuichi
S
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D001439:Bacteroides / Q000502:physiology*; D002454:Cell Differentiation; D018920:Coculture Techniques; D003564:Cytidine Deaminase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D007070:Immunoglobulin A / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D015854:Up-Regulation; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D007070:Immunoglobulin A; D015848:Interleukin-5; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase