Improving the productivity of recombinant protein in Escherichia coli under thermal stress by coexpressing GroELS chaperone system.
Here, we demonstrate that the overexpression of the GroELS chaperone system, which assists the folding of intracellular proteins and prevents aggregation of its biological targets, can enhance the thermotolerance of Escherichia coli strains and facilitate the production of recombinant protein under thermal stress. The overexpression of GroELS led to an about 2-fold higher growth rate of E. coli XL-1 blue than control at 45 degrees and induced the growth of the strain even at 50 degrees , although the growth was not sustained in the second-round culture. The effect of GroELS overexpression was also effective on other E. coli strains such as JM109, DH5alpha, and BL21. Finally, we have shown that coexpression of GroELS allows us to produce recombinant protein even at 50 degrees , a temperature at which the protein production based on E. coli is not efficient. This study indicates that the employment of the GroELS overexpression system can expand the range of environmental conditions for E. coli.
Kim
So-Yeon
SY
Department of Chemical and Biochemical Engineering, and Institute for Environmental Technology and Industry, Pusan National University, Busan, Korea.
Ayyadurai
Niraikulam
N
Heo
Mi-Ae
MA
Park
Sunghoon
S
Jeong
Yong Joo
YJ
Lee
Sun-Gu
SG
D018835:Chaperonin 10 / Q000096:biosynthesis* / Q000737:chemistry; D018834:Chaperonin 60 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006358:Hot Temperature; D018832:Molecular Chaperones / Q000096:biosynthesis / Q000737:chemistry; D015202:Protein Engineering; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015854:Up-Regulation
D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins