Oxidation-triggered release of fluorescent molecules or drugs from mesoporous Si microparticles.
The fluorescent dye Alexa Fluor 488 or the anticancer drug doxorubicin is attached to the surface and inner pore walls of mesoporous Si particles by covalent attachment, and the oxidation-induced release of each molecule is studied. The molecules are bound to the Si matrix using a 10-undecenoic acid linker, which is attached by thermal hydrosilylation. Loading capacity of the microparticles using this method is approximately 0.5 and 45 mg/g of porous Si microparticle for Alexa Fluor 488 and doxorubicin, respectively. The Si-C-bound assembly is initially stable in aqueous solution, although oxidation of the underlying Si matrix results in conversion to silicon oxide and slow release of the linker-molecule complex by hydrolysis of the Si-O attachment points. When the attached molecule is a fluorophore (Alexa Fluor 488 or doxorubicin), its fluorescence is effectively quenched by the semiconducting silicon matrix. As the particle oxidizes in water, the fluorescence intensity of the attached dye increases due to growth of the insulating silicon oxide layer and, ultimately, dye release from the surface. The recovery of fluorescence in the microparticle and the release of the molecule into solution are monitored in real-time by fluorescence microscopy. Both processes are accelerated by introduction of the oxidizing species peroxynitrite to the aqueous solution. The oxidation-triggered release of the anticancer drug doxorubicin to HeLa cells is demonstrated.
Wu
Elizabeth C
EC
Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA.
Park
Ji-Ho
JH
Park
Jennifer
J
Segal
Ester
E
Cunin
Frédérique
F
Sailor
Michael J
MJ
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D004317:Doxorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D010100:Oxygen / Q000737:chemistry*; D010316:Particle Size; D030421:Peroxynitrous Acid / Q000737:chemistry; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
C417561:Alexa 488 hydrazide; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D006834:Hydrazines; D030421:Peroxynitrous Acid; D012822:Silicon Dioxide; D004317:Doxorubicin; D010100:Oxygen; D012825:Silicon