title: Polymorphonuclear cells stimulate the migration and metastatic potential of rat sarcoma cells.
abstract: The tumour microenvironment, which is largely composed of inflammatory cells, is a crucial participant in the neoplastic process through the promotion of cell proliferation, survival and migration. Neutrophil polymorphonuclear cells (PMNs) induce inflammatory reactions that can be either cytotoxic for tumour cells or can promote tumour growth and metastasis. Previously, we have reported a spontaneous metastasis tumour model that has tumour PMNs infiltration, and metastasis, to liver and spleen. The aim of this study was to evaluate the PMNs influences on the tumour cell invasion and metastatic properties. We analysed intercellular adhesion molecule-1 (ICAM-1), urokinase-type plasminogen activator receptor (uPAR), MT1-MMP (membrane type 1-matrix metalloproteinase) and MMP2 protein expression in TuE-t cells cultured with PMNs or PMNs-conditioned medium isolated from tumour bearing and normal rats. The interaction between tumour cells and PMNs induced a decrease in ICAM-1 expression in tumour cells as well as an increase in MMP2 and tumour cell motility. Besides, conserved expression of uPAR and MT1-MMP in tumour cells was also demonstrated. The up-regulation in MMP2 associated with uPAR and MT1-MMP conserved expression may be related to an increased extracellular matrix proteolysis. These results showed that the interaction of tumour cells with PMNs could favour tumour cell spreading through the promotion of a tumour invasive phenotype.
authors.affiliation: Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. mremedi@bioclin.fcq.unc.edu.ar
authors.forename: María Mónica
authors.initials: MM
authors.lastname: Remedi
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Donadio
authors.forename: Gustavo Alberto
authors.initials: GA
authors.lastname: Chiabrando
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism; D009368:Neoplasm Transplantation; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000473:pathology / Q000556:secondary*; D014945:Wound Healing / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; D055293:Receptors, Urokinase Plasminogen Activator; D018799:Intercellular Adhesion Molecule-1; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D053511:Matrix Metalloproteinase 14