title: Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization.
abstract: We discovered a novel method to prepare a protein-based hydrogel, that is, a "Three-Dimensional Nanostructured Protein Hydrogel (3D NPH)", which is composed of protein-polymer hybrid nanoparticles. In this study, we propose a novel protein microarray whose 3D NPH spots were prepared by dispensing a small volume of the solution of protein-polymer mixture on a substrate. The dispensed solution had a short time for cross-linking before its drying-up and the resulting 3D NPH had loosely cross-linked, thin spongy structure. Therefore, the reaction ratio between ligands and analytes was drastically improved in this system compared with the large volume system for Surface Plasmon Resonance (SPR) protein microarray.
authors.affiliation: Graduate School of Science and Technology, Keio University, Yokohama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Minako
authors.initials: M
authors.lastname: Hanasaki
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Isojima
authors.forename: Hisao
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Shiroya
authors.forename: Haruma
authors.initials: H
authors.lastname: Kawaguchi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D021621:Imaging, Three-Dimensional / Q000379:methods*; D055767:Immobilized Proteins / Q000737:chemistry*; D008024:Ligands; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D055767:Immobilized Proteins; D008024:Ligands; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D019809:Streptavidin