DNA-directed self-assembly of fluorescent dye-labeled streptavidin arrays for protein detection.
In this work, a DNA lattice was utilized to direct protein self-assembly. A model biosensor system was fabricated by self-assembly of rhodamine dye-labeled streptavidin molecules on a two-tile DNA system. This self-assembly nanofabrication technique has numerous possible biotechnology applications, including use in multiplex biosensing systems that have binding of several biological molecules encoded in a multiple-tile DNA lattice.
Zhang
Junping
J
Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA.
Narayan
Roger J
RJ
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry*; D019809:Streptavidin / Q000032:analysis* / Q000737:chemistry; D013499:Surface Properties
D046911:Macromolecular Substances; D012235:Rhodamines; D004247:DNA; D019809:Streptavidin