Melanoma cell differentiation induced by lupeol separates into two stages: morphological and functional changes.
Electron microscopic observation revealed that lupeol induced melanosome maturation in B16 2F2 mouse melanoma cells and we therefore studied the effects of lupeol on the intracellular events responsible for melanosome transport. Incubation with lupeol for 8 h attenuated the actin stress fiber assembly in B16 2F2 mouse melanoma cells, resulting in dendritic formation in the cells. Longer exposure to lupeol (48 h) increased the expression of tyrosinase, MITF (a specific transcription factor for tyrosinase), Rab27a, and myosin-Va, which are required for melanosome transport.
Ogiwara
Kikumi
K
Department of Pathology, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa, 229-8501, Japan.
Hata
Keishi
K
D000199:Actins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D014442:Monophenol Monooxygenase / Q000378:metabolism; D053978:Pentacyclic Triterpenes; D014315:Triterpenes / Q000494:pharmacology*; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D000075745:rab27 GTP-Binding Proteins; D000094042:Lupanes
D000199:Actins; D051739:Microphthalmia-Associated Transcription Factor; D014442:Monophenol Monooxygenase; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D020691:rab GTP-Binding Proteins; D000075745:rab27 GTP-Binding Proteins; C497235:Mitf protein, mouse; C512962:Rab27a protein, mouse; C010480:lupeol; D000094042:Lupanes