Establishment of the regeneration system for Vicia faba L.
A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% sucrose and 0.025% (w/v) for each of ascorbic and citric acid, 0.8% agar and different concentrations of 10 mg/l BAP, 0.5 mg/l of each NAA and 2,4-dichlorophenoxyacetic acid (M1) and 1 mg/l BAP and 0.5 mg/l NAA (M2) . The media with BAP, NAA and 2,4-D were optimal for embryogenic callus induction. Somatic embryos developed after transfer of the callus to 1/2 B5 medium with no plant growth regulators. There were various stages of somatic embryo development present including globular, heart-shaped, torpedo, and cotyledonary stages. Embryos developed into plantlets and plants were regenerated. RAPD analyses were performed to investigate the genetic stability of the regenerated plants obtained from different treatments and different explants. The cultivar Giza 2 exhibited more genetic stability than cultivar 24 Hyto. In conclusion, a regeneration system was established suitable for both gene transformation and the isolation of somaclonal mutants. The regeneration system will be used in order to improve the nutritional value of faba bean.
Bahgat
Shimaa
S
Faculty of Agriculture Research Park (FARP) and Biochemistry Department, Faculty of Agriculture, University of Cairo, 12613 Giza, Egypt.
Shabban
Omer A
OA
El-Shihy
Osama
O
Lightfoot
David A
DA
El-Shemy
Hany A
HA
D002478:Cells, Cultured; D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology*; D018520:Plant Shoots / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D046509:Tissue Culture Techniques; D031307:Vicia faba / Q000196:embryology / Q000235:genetics / Q000502:physiology*
D003470:Culture Media; D010937:Plant Growth Regulators