TOX3 regulates calcium-dependent transcription in neurons.
We report the cloning and characterization of TOX3, a high mobility group box protein involved in mediating calcium-dependent transcription. TOX3 was identified as a calcium-dependent transactivator using the Transactivator Trap screen. We find that TOX3 interacts with both cAMP response element (CRE)-binding protein (CREB) and CREB-binding protein (CBP), and knockdown of the endogenous TOX3 by RNAi leads to significant reduction of calcium-induced c-fos expression and complete inhibition of calcium activation of the c-fos promoter. The effects of TOX3 on calcium-dependent transcription require the CRE elements. These observations identify TOX3 as an important regulator of calcium-dependent transcription and suggest that TOX3 exerts its effect on CRE-mediated transcription via its association with the CREB-CBP complex.
Yuan
Shauna H
SH
Department of Neuroscience and Neurobiology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0366.
Qiu
Zilong
Z
Ghosh
Anirvan
A
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D050882:CREB-Binding Protein / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D016762:Genes, fos; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D020318:Rats, Long-Evans; D014158:Transcription, Genetic / Q000502:physiology*
D017362:Cyclic AMP Response Element-Binding Protein; D017931:DNA Primers; D015500:Chloramphenicol O-Acetyltransferase; D050882:CREB-Binding Protein; D002118:Calcium