title: Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney.
abstract: OBJECTIVE
  To compare the effect of short-term calorie restriction (CR) on aging with that of already known long-term CR, the anti-inflammatory efficacy of 10-day CR was explored in aged rat kidney.
  
  
  METHODS
  Two different age groups, 6 months (young) and 24 months (old) were used. In the old group, one sub-group was control, fed ad libitum (AL) and the other was fed CR for 10 days with 40 % of the food intake of the AL subgroup (n = 5).
  
  
  METHODS
  Reactive species (RS), lipid peroxides and COX-2 activity were measured. The activities of proinflammatory transcription factors NF-kB and AP-1 were measured by electro-mobility shift assay (EMSA). Upstream signaling cascades of NF-kB and AP-1 as well as proinflammatory gene expression were detected by Western blot.
  
  
  RESULTS
  10-day CR suppressed RS, lipid peroxides, and COX-2 activity in aged rat kidney. CR also inhibited upstream signaling cascades and DNA binding activity of NF-kB and AP-1, and thioredoxin/Ref-1 pathway. CR blocked expression of NF-kB-and AP-1-responsive gene COX-2, iNOS, VCAM-1 and ICAM-1.
  
  
  CONCLUSIONS
  We report for the first time that 10-day CR can attenuate the altered signaling transduction for inflammatory processes which is mediated through RS-induced NF-kB and AP-1 in aged kidney.
authors.affiliation: Department of Pharmacy, Pusan National University, Busan, Korea.
authors.forename: K J
authors.initials: KJ
authors.lastname: Jung
authors.forename: E K
authors.initials: EK
authors.lastname: Lee
authors.forename: J Y
authors.initials: JY
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Zou
authors.forename: B
authors.initials: B
authors.lastname: Sung
authors.forename: H S
authors.initials: HS
authors.lastname: Heo
authors.forename: M K
authors.initials: MK
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: N D
authors.initials: ND
authors.lastname: Kim
authors.forename: B Pal
authors.initials: BP
authors.lastname: Yu
authors.forename: H Y
authors.initials: HY
authors.lastname: Chung
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D031204:Caloric Restriction; D004032:Diet; D007249:Inflammation / Q000276:immunology*; D007668:Kidney / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D018808:Transcription Factor AP-1 / Q000378:metabolism*
chemical_list: D052002:NF-kappa B p50 Subunit; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D011451:Prostaglandin-Endoperoxide Synthases; D020928:Mitogen-Activated Protein Kinases