Hippocampal theta rhythm and its coupling with gamma oscillations require fast inhibition onto parvalbumin-positive interneurons.
Hippocampal theta (5-10 Hz) and gamma (35-85 Hz) oscillations depend on an inhibitory network of GABAergic interneurons. However, the lack of methods for direct and cell-type-specific interference with inhibition has prevented better insights that help link synaptic and cellular properties with network function. Here, we generated genetically modified mice (PV-Deltagamma(2)) in which synaptic inhibition was ablated in parvalbumin-positive (PV+) interneurons. Hippocampal local field potential and unit recordings in the CA1 area of freely behaving mice revealed that theta rhythm was strongly reduced in these mice. The characteristic coupling of theta and gamma oscillations was strongly altered in PV-Deltagamma(2) mice more than could be accounted for by the reduction in theta rhythm only. Surprisingly, gamma oscillations were not altered. These data indicate that synaptic inhibition onto PV+ interneurons is indispensable for theta- and its coupling to gamma oscillations but not for rhythmic gamma-activity in the hippocampus. Similar alterations in rhythmic activity were obtained in a computational hippocampal network model mimicking the genetic modification, suggesting that intrahippocampal networks might contribute to these effects.
Wulff
Peer
P
Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom.
Ponomarenko
Alexey A
AA
Bartos
Marlene
M
Korotkova
Tatiana M
TM
Fuchs
Elke C
EC
Bähner
Florian
F
Both
Martin
M
Tort
Adriano B L
AB
Kopell
Nancy J
NJ
Wisden
William
W
Monyer
Hannah
H
D000818:Animals; D001522:Behavior, Animal; D004594:Electrophysiology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007395:Interneurons / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008959:Models, Neurological; D010320:Parvalbumins / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D021122:Protein Subunits / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism; D013569:Synapses / Q000187:drug effects / Q000378:metabolism; D013826:Theta Rhythm; D013997:Time Factors
D010320:Parvalbumins; D021122:Protein Subunits; D011963:Receptors, GABA-A