The anti-condyloma acuminatum effects of interferon-inducible protein 10 in vitro.
BACKGROUND
Interferon-inducible protein 10 (IP-10) has been reported to show an effective antiviral and antineoplastic role in various murine models.


OBJECTIVE
To investigate the anti-condyloma acuminatum effect and human papillomavirus (HPV)-inhibiting efficacy of a recombinant plasmid encoding IP-10 in vitro.


METHODS
A recombinant plasmid DNA carrying IP-10 cDNA was constructed. Condyloma acuminatum tissue particles were transfected with IP-10 and examined for apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry techniques. Relative real-time polymerase chain reaction (PCR) was used to validate the HPV inhibited level of the treatment groups. Immunohistochemistry was used to determine the IP-10 expression on condyloma acuminatum tissues.


RESULTS
Transfected IP-10 was expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful apoptosis was observed in condyloma acuminatum tissues transfected with IP-10. In addition, HPV expression was lower in IP-10-treated tissues than in control tissues.


CONCLUSIONS
These observations suggest that IP-10 has strong anti-condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and therefore may be a novel and potentially effective therapy for condyloma acuminatum.
Li
Huijuan
H
Department of Gynecology and Obstetrics, West China Second University Hospital, and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Yi
Tao
T
Zhao
Shuyun
S
Chen
Pin
P
Cheng
Chenxian
C
Wei
Yuquan
Y
Zhao
Xia
X
D000328:Adult; D017209:Apoptosis; D054357:Chemokine CXCL10 / Q000235:genetics*; D003218:Condylomata Acuminata / Q000628:therapy* / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D052140:Human papillomavirus 11; D052163:Human papillomavirus 6; D006801:Humans; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D011994:Recombinant Proteins