A noninvasive method for measuring the oxygen binding-releasing capacity of hemoglobin-loaded polymeric nanoparticles as oxygen carrier.
Based on the strong penetration capacity of near infrared lights (NIRs) and different absorption of oxyhemoglobin and deoxyhemoglobin in NIRs region, a novel noninvasive method, with the aid of an airproof-equilibrium apparatus, was developed to determine the oxygen binding-releasing capacity, including oxygen dissociation curve (ODC) and P(50), of the hemoglobin-loaded polymeric nanoparticles (HbP) in this study. The measured ODC of the PLA-PEG HbP was very close to that of the native hemoglobin, and the corresponding P(50) (26.1 mmHg) was also near to the native precursor protein (27.3 mmHg), indicative of the validity of the method proposed. To further verify the method proposed, the oxygen binding-releasing capacity of the HbPs prepared by PCL, PCL-PEG, PLA were also investigated with human blood as control. These results indicated that the method developed here enabled accurate and noninvasive determination of the oxygen binding-releasing capacity of the biodegradable polymeric oxygen carriers.
Zhang
Xiaolan
X
Key Laboratory for Ultrafine Materials of Ministry of Education, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China. zxlpsh@126.com
Liu
Changsheng
C
Yuan
Yuan
Y
Shan
Xiaoqian
X
Sheng
Yan
Y
Xu
Feng
F
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D001802:Blood Substitutes / Q000378:metabolism*; D002417:Cattle; D006454:Hemoglobins / Q000378:metabolism*; D066298:In Vitro Techniques; D008422:Materials Testing / Q000295:instrumentation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
D001672:Biocompatible Materials; D001802:Blood Substitutes; D006454:Hemoglobins; D011108:Polymers; D010100:Oxygen