Inflammatory and oxidative stress markers in experimental crystalopathy: their modification by photostimulation.
Crystalopathies are inflammatory pathologies caused by cellular reactions to the deposition of crystals in the joints. The anti-inflammatory effect of the helium-neon (He-Ne) laser and that of the nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac, meloxicam, celecoxib, and rofecoxib was studied in acute and chronic arthritis produced by hydroxyapatite and calcium pyrophosphate in rats. The presence of the markers fibrinogen, L-citrulline, nitric oxide, and nitrotyrosine was determined. Crystals were injected into the posterior limb joints of the rats. A dose of 8 J/cm(2) of energy from an He-Ne laser was applied for 3 d in some groups and for 5 d in other groups. The levels of some of the biomarkers were determined by spectrophotometry, and that of nitrotyrosine was determined by ELISA. For statistical analysis, Fisher's exact test was used, and p +/- 0.05 was considered significant. In arthritic rats, the fibrinogen, L-citrulline, nitric oxide, and nitrotyrosine levels increased in comparison to controls and to the laser-treated arthritic groups (p +/- 0.001), (p +/- 0.001), (p +/- 0.02), and (p +/- 0.01), respectively. When comparing fibrinogen from arthritic rats with disease induced by hydroxyapatite with undiseased and arthritic rats treated with NSAIDs, the He-Ne laser decreased levels to values similar to those seen in controls (p +/- 0.01). Inflammatory and oxidative stress markers in experimental crystalopathy are positively modified by photobiostimulation.
Rubio
Claudia Reinoso
CR
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Simes
Juan Carlos
JC
Moya
Monica
M
Soriano
Fernando
F
Palma
Jose Atilio
JA
Campana
Vilma
V
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001168:Arthritis / Q000188:drug therapy / Q000378:metabolism / Q000532:radiotherapy*; D015415:Biomarkers / Q000097:blood; D002131:Calcium Pyrophosphate / Q000008:administration & dosage; D002956:Citrulline / Q000097:blood; D003460:Crystallization; D017886:Durapatite / Q000008:administration & dosage; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006614:Hindlimb; D007249:Inflammation; D007270:Injections, Intra-Articular; D054020:Lasers, Gas / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D051381:Rats
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D002956:Citrulline; D009569:Nitric Oxide; D005340:Fibrinogen; D017886:Durapatite; D002131:Calcium Pyrophosphate