title: Sunflower protein hydrolysates reduce cholesterol micellar solubility.
abstract: Plant protein hydrolysates are a source of bioactive peptides. There are peptides that decrease the micellar cholesterol solubility from bile acids and therefore may reduce in vivo cholesterol absorption. The presence of these peptides in sunflower protein hydrolysates has been studied. Sunflower protein hydrolysates produced with alcalase plus flavourzyme or with pepsin plus pancreatin inhibited in some degree the cholesterol incorporation to micelles. Protein hydrolysates generated after 30 min of hydrolysis with alcalase, and after 30 min of hydrolysis with pepsin, were the inhibitoriest of the cholesterol incorporation to micelles. The average amino acid hydrophobicity of inhibitory peptides in cholesterol micelles was higher than the observed in the corresponding protein hydrolysates. This high hydrophobicity probably favours their inclusion in the lipid micelles. In vivo, this inhibition may translate in a decrease of cholesterol absorption. Reported results show that a combination of different characteristics such as peptide size or hydrophobicity may be responsible of the inhibitory activity of generated peptides.
authors.affiliation: Instituto de la Grasa (C.S.I.C.), Padre García Tejero 4, Sevilla 41012, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Megías
authors.forename: Justo
authors.initials: J
authors.lastname: Pedroche
authors.forename: María
authors.initials: M
authors.lastname: Del Mar Yust
authors.forename: Manuel
authors.initials: M
authors.lastname: Alaiz
authors.forename: Julio
authors.initials: J
authors.lastname: Girón-Calle
authors.forename: Francisco
authors.initials: F
authors.lastname: Millán
authors.forename: Javier
authors.initials: J
authors.lastname: Vioque
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002784:Cholesterol / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D006368:Helianthus; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D010194:Pancreatin / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility
chemical_list: D000596:Amino Acids; D008823:Micelles; D010455:Peptides; D010940:Plant Proteins; D011492:Protein Hydrolysates; D010194:Pancreatin; D002784:Cholesterol; D010450:Endopeptidases; D010447:Peptide Hydrolases