Aspirin prevents diabetic oxidative changes in rat lacrimal gland structure and function.
The aim of this study is to evaluate whether aspirin reduces Diabetis Mellitus (DM) oxidative damage in the lacrimal gland (LG), and ocular surface (OS). Ten weeks after streptozotocin induced DM and aspirin treatment, LG and OS of rats were compared for tear secretion, hidtology, peroxidase activity, and expression of uncoupling proteins (UCPs). DM reduction of tear secretion was prevented by aspirin (P < 0.01). Alterations of LG morphology and increased numbers of lipofucsin-like inclusions were observed in diabetic but not in aspirin-treated diabetic rats. Peroxidase activity levels were higher and UCP-2 was reduced in DM LG but not in aspirin treated (P = 0.0025 and P < 0.05, respectively). The findings prevented by aspirin indicate a direct inhibitory effect on oxidative pathways in LG and their inflammatory consequences, preserving the LG structure and function against hyperglycemia and/or insulin deficiency damage.
Jorge
Angélica Gobbi
AG
Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço e, FMRP, Universidade de São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil.
Módulo
Carolina Maria
CM
Dias
Ana Carolina
AC
Braz
Alexandre Martins
AM
Filho
Rubens Bertazolli
RB
Jordão
Alceu A
AA
de Paula
Jayter Silva
JS
Rocha
Eduardo Melani
EM
D000818:Animals; D001241:Aspirin / Q000627:therapeutic use*; D002467:Cell Nucleus / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000188:drug therapy*; D007473:Ion Channels / Q000032:analysis; D007765:Lacrimal Apparatus / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D024101:Mitochondrial Proteins / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013666:Tears / Q000378:metabolism; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604391:Ucp2 protein, rat; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3; D009195:Peroxidase; D001241:Aspirin