[Determination of two saponins in Tupistra chinensis rhizomes by RP-HPLC].
OBJECTIVE
To develog an HPLC method determination of two new saponins in the dried rhizomes of Tupistra chinensis.


METHODS
The analysis was performed on YMC-Pack ODS-AQ (4.6 mm x 250 mm, 5 microm) eluted with of acetonitrile and waterin gradient mode. The conentration of acetonitrile in the mobile phase changes from 10% to 100% within 85 minutes. The detection wavelength was set at 203 nm. The flow rate was 1.00 mL x min(-1) and column temperature was set at 30 degrees C.


RESULTS
The linear relationships of two authentic saponins were determined within the range from 7.55 microg to 60.40 microg (r=0.9996) and 8.00 microg to 48.00 microg (r=0.9996), respectively. Using the method above, the contents of two saponins were determined as 0.261% and 0.242%, with the recoveries as 98.6% and 98.3% with RSD 1.7% and 1.7%, respectively.


CONCLUSIONS
A convenient and reliable method was developed to determine the content of two saponins in the dried rhizomes of Tupistra chinensis.
Zhou
Yuan
Y
Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Sciences, China Three Gorges University, Yichang 443002, China.
Zou
Kun
K
Yu
Ling-ling
LL
Qin
Shuang-jiu
SJ
Xu
Lan-lan
LL
Liu
Chuang
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000737:chemistry*
D012503:Saponins