title: Baboon carboxylesterases 1 and 2: sequences, structures and phylogenetic relationships with human and other primate carboxylesterases.
abstract: BACKGROUND
  Carboxylesterase (CES) is predominantly responsible for the detoxification of a wide range of drugs and narcotics, and catalyze several reactions in cholesterol and fatty acid metabolism. Studies of the genetic and biochemical properties of primate CES may contribute to an improved understanding of human disease, including atherosclerosis, obesity and drug addiction, for which non-human primates serve as useful animal models.
  
  
  METHODS
  We cloned and sequenced baboon CES1 and CES2 and used in vitro and in silico methods to predict protein secondary and tertiary structures, and examined evolutionary relationships for these enzymes with other primate and mouse CES orthologs.
  
  
  CONCLUSIONS
  We found that baboon CES1 and CES2 proteins retained extensive similarity with human CES1 and CES2, shared key structural features reported for human CES1, and showed family specific sequences consistent with their multimeric and monomeric subunit structures respectively.
authors.affiliation: Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78227, USA.
authors.forename: Roger S
authors.initials: RS
authors.lastname: Holmes
authors.forename: Jeremy P
authors.initials: JP
authors.lastname: Glenn
authors.forename: John L
authors.initials: JL
authors.lastname: VandeBerg
authors.forename: Laura A
authors.initials: LA
authors.lastname: Cox
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D043182:Carboxylesterase / Q000737:chemistry / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D010215:Papio / Q000235:genetics*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D021122:Protein Subunits; D043182:Carboxylesterase