Dithiolane linked thiorhodamine dimer for Hg2+ recognition in living cells.
Thiorhodamine-based chemodosimeter A, a disulfide linked dimer, was designed for Hg2+ recognition by virtue of the strong affinity of mercury for sulfur. Spectroscopic results reveal that chemodosimeter A exhibits real-time responses, and high sensitivity and selectivity for Hg2+ in comparison to other cations. These properties are mechanistically ascribed to the transfer from rhodamine spirolactam to the thiazoline-derived open-ring rhodamine via Hg2+ induced desulfurization. The in vitro recognition of Hg2+ in living cells pretreated with A was examined, showing that the concentration of Hg2+ that could be imaged reaches the safety limit for human beings.
Liu
Weimin
W
Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Physics and Chemistry, the Chinese Academy of Sciences, Beijing, 100190, China.
Xu
Liwei
L
Zhang
Hongyan
H
You
Juanjuan
J
Zhang
Xiaoling
X
Sheng
Ruilong
R
Li
Huaping
H
Wu
Shikang
S
Wang
Pengfei
P
D002477:Cells / Q000737:chemistry*; D002478:Cells, Cultured; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry; D015386:Hazardous Substances / Q000032:analysis; D006801:Humans; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D004220:Disulfides; D015386:Hazardous Substances; D012235:Rhodamines; D013438:Sulfhydryl Compounds; D008628:Mercury