title: Differential microRNA expression in childhood B-cell precursor acute lymphoblastic leukemia.
abstract: MiRNAs play important roles in the development of both hematopoiesis and leukemogenesis. The analysis of differential microRNA expression profiles may be a powerful tool to allow us insight on the mechanisms of childhood B-cell precursor acute lymphoblastic leukemia (pre-B-ALL). The present study provides an informative profile of the expression of miRNAs in pre-B-ALL using two independent and quantitative methods: miRNA chip and qRT-PCR of mature miRNA from 40 newly diagnosed pre-B-ALL children. Additionally, putative hematopoiesis-specific target genes were analyzed with informatics technique. Both approaches showed that miR-222, miR-339, and miR-142-3p were dramatically overexpressed in pre-B-ALL patients, and downregulation of hsa-miR-451 and hsa-miR-373* was confirmed. The results of this study offer a comprehensive and quantitative profile of miRNA expression in pre-B-ALL and their healthy counterpart, suggesting that miRNAs could play a primary role in the disease itself.
authors.affiliation: Cryomedicine Laboratory, Qilu Hospital of Shandong University, Shandong, China. shellysdcn07@hotmail.com
authors.forename: Xiuli
authors.initials: X
authors.lastname: Ju
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Shi
authors.forename: Huaishui
authors.initials: H
authors.lastname: Hou
authors.forename: Nianzheng
authors.initials: N
authors.lastname: Sun
authors.forename: Baijun
authors.initials: B
authors.lastname: Shen
mesh_terms: D002648:Child; D002675:Child, Preschool; D019295:Computational Biology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D007223:Infant; D035683:MicroRNAs / Q000235:genetics*; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics
chemical_list: D035683:MicroRNAs; C525710:MIR222, human; C527114:MIRN373 microRNA, human; C531201:MIRN451 microRNA, human