title: Isolation, Amino-Acid Sequence, Synthesis and Biological Properties of Urotensin I from Hippoglossoides elassodon.
abstract: Abstract A 41-residue urotensin I neuropeptide (H-UI) was isolated from urophyses of the marine teleost Hippoglossoides elassodon (the flathead sole). The peptide was recognized by its partial cross-reactivity in a radioimmunoassay developed for Catostomus (sucker) Ul (S-UI), and was purified by reversed-phase high-performance liquid chromatography. The amino-acid sequence was shown to be H-Ser-Glu-Glu-Pro-Pro-Met-Ser-lle-Asp-Leu-Thr-Phe-His-Met-Leu-Arg-Asn-Met-lle-His-Arg-Ala-Lys-Met-Glu-Gly-Glu-Arg-Glu-Gln-Ala-Leu-lle-Asn-Arg-Asn-Leu-Leu-Asp-Glu-Val-NH(2). H-UI is 66% homologous with S-UI and 63% homologous with Cyprinus (carp) Ul (C-UI). Like S- and C-UI, H-UI is about 50% homologous with the frog skin peptide sauvagine and with Catostomus and mammalian corticotropin-releasing factors. H-UI had similar vasodilatory effects in mammals, and similar adrenocorticotropin-releasing effects (in rat and goldfish) to S-UI, C-UI, sauvagine and the corticotropin-releasing factors, but had relatively low potency (e.g. 10% to 30% of the vasodilatory potency of S- and C-UI) in all the bioassay systems studied.
authors.affiliation: Department of Pharmacology and Therapeutics, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1.
authors.forename: D
authors.initials: D
authors.lastname: McMaster
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: K L
authors.initials: KL
authors.lastname: Maccannell
authors.forename: J
authors.initials: J
authors.lastname: Rivier
authors.forename: C
authors.initials: C
authors.lastname: Rivier
authors.forename: W
authors.initials: W
authors.lastname: Vale
authors.forename: J N
authors.initials: JN
authors.lastname: Fryer
authors.forename: T N
authors.initials: TN
authors.lastname: Tran
authors.forename: K
authors.initials: K
authors.lastname: Lederis