Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two monoclonal antibodies.
An immunoaffinity chromatographic method was developed for the purification of 9 sulfonamides (sulfamethazine, sulfadimethoxine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamonomethoxine, and sulfapyridine) from chicken tissue (muscle and liver) samples. Two monoclonal antibodies (antisulfamethazine and antisulfamethoxazole) were simultaneously covalently coupled to CNBr-activated Sepharose 4B for the preparation of a re-usable immunoaffinity column. After extraction with methanol-water and purification by immunoaffinity column, the sulfonamides were determined by reversed-phase liquid chromatography and UV detection at 270 nm. The recoveries for each drug at fortification levels of 10-50 ng/g ranged from 74.1 to 108.9% with relative standard deviations from 1.9 to 11.5%. The limits of detection were 2 ng/g for each drug.
Li
Bingyu
B
China Agricultural University, National Reference Laboratory for Veterinary Drug Residue, Department of Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100094, People's Republic of China.
Li
Cun
C
Jiang
Haiyang
H
Wang
Zhahnui
Z
Cao
Xingyuan
X
Zhao
Sijun
S
Zhang
Suxia
S
Shen
Jianzhong
J
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002138:Calibration; D002645:Chickens / Q000378:metabolism*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D012015:Reference Standards; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000032:analysis* / Q000302:isolation & purification
D000890:Anti-Infective Agents; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D012996:Solutions; D013449:Sulfonamides