title: Single-step synthesis of 4-nitrophenyl ferulate for spectrophotometric assay of feruloyl esterases.
abstract: We report here, for the first time, a single-step method for the synthesis of 4-nitrophenyl ferulate (4NPF), a spectrophotometric substrate for the assay of feruloyl esterases by dehydrative coupling of ferulic acid and 4-nitrophenol.
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
authors.forename: Shyamala
authors.initials: S
authors.lastname: Hegde
authors.forename: Pullabhatla
authors.initials: P
authors.lastname: Srinivas
authors.forename: Gudipati
authors.initials: G
authors.lastname: Muralikrishna
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D003373:Coumaric Acids / Q000138:chemical synthesis* / Q000737:chemistry; D009574:Nitro Compounds / Q000138:chemical synthesis*; D009596:Nitrophenols / Q000737:chemistry
chemical_list: C546405:4-nitrophenyl ferulate; D003373:Coumaric Acids; D009574:Nitro Compounds; D009596:Nitrophenols; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; C024836:4-nitrophenol