Structural analysis of glycosaminoglycans and proteoglycans by means of Raman microspectrometry.
Raman spectra have been determined for hyaluronan, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan monomers and aggregates. The nature of the saccharides and the pattern of sulfation can be discerned. There were only small spectral changes with pH and ionic composition. Differences between hydroxyl vibrations, bulk water and solution conditions are shown. The spectrum of aggrecan is dominated by chondroitin sulfate contribution. The sulfation pattern and ratio of protein to glycosaminoglycan and the secondary structure of the core protein were determined.
Ellis
Richard
R
School of Physics, University of Exeter, Exeter, United Kingdom. R.E.Ellis@exeter.ac.uk
Green
Ellen
E
Winlove
C Peter
CP
D006025:Glycosaminoglycans / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011509:Proteoglycans / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013431:Sulfates / Q000737:chemistry; D014867:Water / Q000737:chemistry
D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D014867:Water