Solid-phase molecularly imprinted pre-concentration and spectrophotometric determination of isoxicam in pharmaceuticals and human serum.
A selective molecularly imprinted polymer (MIP) has been synthesized for isoxicam pre-concentration, followed by its spectrophotometric determination based on hydrogen bonding interactions between examined drug and alizarin yellow GG. This method is able to evaluate isoxicam in range of 1.0 x 10(-3) to 20.0 microg mL(-1), with a limit of determination of 1.0 ng mL(-1). The retention capacity and pre-concentration factor of prepared sorbent are 18.5 mg g(-1) and 200, respectively; and the prepared MIPs can be reused at least for five times. The MIP capability for isoxicam selection and extraction from the solution is higher than non-imprinted polymer (NIP). Under optimum conditions, this procedure can be successfully applied to assay trace amounts of isoxicam in pharmaceutical and biological samples.
Rezaei
B
B
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. rezaei@cc.iut.ac.ir
Mallakpour
S
S
Majidi
N
N
D001391:Azo Compounds; D006860:Hydrogen Bonding; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000032:analysis; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood
D001391:Azo Compounds; D004364:Pharmaceutical Preparations; D010894:Piroxicam; C039508:alizarine yellow G; C013580:isoxicam