title: Microchip capillary electrophoresis.
abstract: Microchip capillary electrophoresis (MCE) is gaining popularity due to the developments of simple microfabrication methods under nonstringent laboratory conditions. Moreover, the low material and production costs of polymer-based microchips have further stimulated advances in the applications of MCE in various fields, including clinical analysis, drug screening, biomarker identification, and biosensing. In this chapter, a simple and robust protocol for fabrication of microchips for lab-on-chip testing and microchip electrophoresis is described. The microchips are hybrid poly(dimethylsiloxane) (PDMS)/glass microchips, which are produced by a combination of photolithography and micromolding processes. This type of microchip has been used in a wide range of analyses.
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore, Republic of Singapore.
authors.forename: Elaine T T
authors.initials: ET
authors.lastname: Tay
authors.forename: Wai S
authors.initials: WS
authors.lastname: Law
authors.forename: Sam F Y
authors.initials: SF
authors.lastname: Li
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Kricka
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods*