title: [Molecular characterization of calicivirus strains detected in outbreaks of gastroenteritis occurring in Argentina during 2005 and 2006].
abstract: In order to determine the incidence of calicivirus, rotavirus and astrovirus in outbreaks of gastroenteritis occurring in different regions of Argentina during 2005 and 2006, fecal samples from seven nonbacterial outbreaks were analyzed. A commercial ELISA was used for rotavirus detection, while RT-PCRs were used for calicivirus and astrovirus. Of the 74 samples analyzed, 20 were calicivirus positive, 17 were rotavirus positive and one was astrovirus positive. No mixed infections were detected. A partial region of the RdRp gene was sequenced in five calicivirus positive-samples; 4 of them belonged to Norovirus genus and one to Sapovirus genus. The phylogenetic analysis of norovirus-positive-samples revealed the presence of strains from genogroups GI and GII; genotypes GII-4, GII-b and GII-17 were identified within the latter. Phylogenetic the sapovirus-positive-sample revealed the presence of genotype GI-1. This study represents a follow-up of the of molecular epidemiology analysis of calicivirus associated to gastroenteritis outbreaks that have been carried out by our group since 2004, and constitutes the first report of the circulation of genotype GII-17 in Argentina.
authors.affiliation: Laboratorio de Gastroenteritis Virales, Departamento de Virología, INEI-ANLIS Dr. Carlos G. Malbán, Ciudad Autónoma de Buenos Aires, Argentina. kgomes@anlis.gov.ar
authors.forename: K A
authors.initials: KA
authors.lastname: Gomes
authors.forename: J A
authors.initials: JA
authors.lastname: Stupka
authors.forename: A
authors.initials: A
authors.lastname: Diana
authors.forename: G I
authors.initials: GI
authors.lastname: Parra
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001118:Argentina / Q000453:epidemiology; D019350:Astroviridae Infections / Q000453:epidemiology / Q000821:virology; D001483:Base Sequence; D002139:Caliciviridae / Q000235:genetics / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D001257:Mamastrovirus / Q000302:isolation & purification; D008969:Molecular Sequence Data; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D029341:Sapovirus / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral