2-Aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline.
2-Aminopurine-modified abasic-site-containing duplex [DNA 5'-TCTGC GTCCT PXT TAACG CACAC-3'/3'-AGACG CAGGA TCA ATTGC GTGTG-5'; P = 2-aminopurine, X = abasic site (Spacer-C3), C = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 microM (5 degrees C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.
Li
Minjie
M
Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
Sato
Yusuke
Y
Nishizawa
Seiichi
S
Seino
Takehiro
T
Nakamura
Kodai
K
Teramae
Norio
N
D015075:2-Aminopurine / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013806:Theophylline / Q000032:analysis* / Q000737:chemistry
D052157:Aptamers, Nucleotide; D015342:DNA Probes; D005456:Fluorescent Dyes; D015075:2-Aminopurine; D004247:DNA; D013806:Theophylline