Monocyte-derived dendritic cells from patients with severe forms of chromoblastomycosis induce CD4+ T cell activation in vitro.
Dendritic cells (DCs) have been described as initiators and modulators of the immune response. Recently we have shown a predominant production of interleukin-10 cytokine, low levels of interferon-gamma and inefficient T cell proliferation in patients with severe forms of chromoblastomycosis. Chromoblastomycosis starts with subcutaneous inoculation of Fonsecaea pedrosoi into tissue where DCs are the first line of defence against this microorganism. In the present study, the interaction of F. pedrosoi and DCs obtained from patients with chromoblastomycosis was investigated. Our results showed that DCs from patients exhibited an increased expression of human leucocyte antigen D-related (HLA-DR) and co-stimulatory molecules. In the presence of conidia, the expression of HLA-DR and CD86 was up-regulated by DCs from patients and controls. Finally, we demonstrate the reversal of antigen-specific anergy and a T helper type 1 response mediated by DCs incubated with F. pedrosoi conidea.
Sousa
M Glória
MG
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de Sao Paulo, SP, Brazil.
Ghosn
E Eid Bou
EE
Nascimento
R Ciciro
RC
Bomfim
G Facchioli
GF
Noal
V
V
Santiago
K
K
de Maria Pedrozo E Silva Azevedo
C
C
Marques
S Garcia
SG
Gonçalves
A Guedes
AG
de Castro Lima Santos
D Wagner
DW
Criado
P Ricardo
PR
Costa Martins
J Eduardo
JE
Almeida
S Rogerio
SR
D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000276:immunology; D051940:B7-2 Antigen / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002862:Chromoblastomycosis / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D003904:Mitosporic Fungi / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology
D000946:Antigens, Fungal; D051940:B7-2 Antigen; D016207:Cytokines; D006684:HLA-DR Antigens