title: Non-viable human embryos as a source of viable cells for embryonic stem cell derivation.
abstract: Human embryonic stem cells (hESC) hold great promise for use in regenerative medicine. However, the extraordinary potential of hESC as therapeutic tools is tempered by ethical, moral and political issues surrounding their derivation from human embryos. It has previously been proposed that ethical criteria applied to essential organ donation could be employed for derivation of hESC from irreversibly arrested, and thus organismically dead, human embryos produced during routine IVF procedures. Here, it is shown that arrested embryos do not resume normal development during extended culture, yet most of them contain a substantial number of living cells on embryonic day 6 (72% have <1 viable cell, 47% have <5 viable cells), suggesting that this class of non-viable embryos could be a rich source of viable cells for derivation of hESC lines.
authors.affiliation: Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Gavrilov
authors.forename: Robert W
authors.initials: RW
authors.lastname: Prosser
authors.forename: Imran
authors.initials: I
authors.lastname: Khalid
authors.forename: Joanne
authors.initials: J
authors.lastname: MacDonald
authors.forename: Mark V
authors.initials: MV
authors.lastname: Sauer
authors.forename: Donald W
authors.initials: DW
authors.lastname: Landry
authors.forename: Virginia E
authors.initials: VE
authors.lastname: Papaioannou
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000502:physiology; D046149:Embryo Culture Techniques; D019924:Embryo Disposition; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D013997:Time Factors