title: Exogenous crosslinking recovers the functional integrity of intervertebral disc secondary to a stab injury.
abstract: Exogenous crosslinking was proved to improve the fatigue resistance of anulus fibrosus and the stability of motion segment. The effect of crosslinking on the recovery of stab-injured discs, however, was less studied. The purpose of this study is to find if the exogenous crosslinking can increase the mechanical function of injured discs. Fresh healthy porcine discs (T2/T9) from 6-month-old swine were obtained immediately following death. Anular puncture using 16 and 18 G spinal needle were used to create medium and large disc stab injury models. Three treatments were designed for each injury model. The first one is the injured discs without treatment. The second one is the injured discs soaked with phosphate buffered solution for 2 days. The third one is the injured discs soaked with 0.33% genipin solution for 2 days. The disc integrity was evaluated using quantitative discomanometry (QD) apparatus. Four QD parameters, that is, the leakage pressure and volume, and the saturate pressure and volume, were analyzed to find the efficacy of treatment. We found that soaking of genipin solution recovered the disc leakage pressure from 1.3 to 1.8 MPa in 16 G-injury-model and from 2.3 to 3.2 MPa in 18 G-injury-model, and recovered the saturate pressure from 1.6 to 2.0 MPa in 16 G-injury-model and from 2.7 to 3.7 MPa in 18 G-injury-model. The improvement of disc integrity by soaking with genipin solution indicate that the exogenous crosslinking may help the biomechanical performance of an injured disc.
authors.affiliation: Department of Orthopaedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Shih-Youeng
authors.initials: SY
authors.lastname: Chuang
authors.forename: Leou-Chyr
authors.initials: LC
authors.lastname: Lin
authors.forename: Yi-Chian
authors.initials: YC
authors.lastname: Tsai
authors.forename: Jaw-Lin
authors.initials: JL
authors.lastname: Wang
mesh_terms: D000818:Animals; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006801:Humans; D007403:Intervertebral Disc / Q000187:drug effects / Q000293:injuries* / Q000503:physiopathology*; D008365:Manometry; D011312:Pressure; D020127:Recovery of Function / Q000187:drug effects*; D034421:Sus scrofa; D013904:Thoracic Vertebrae / Q000187:drug effects / Q000293:injuries / Q000473:pathology / Q000503:physiopathology
chemical_list: D003432:Cross-Linking Reagents