Thymosin beta-10 is aberrantly expressed in pancreatic cancer and induces JNK activation.
Thymosin beta-10 (T beta 10) has been shown to be associated with several cancers; however, its role in pancreatic cancer is not understood. The expression of T beta 10 was determined by immunohistochemistry and real-time polymerase chain reaction. The phosphorylation of JNK and the cytokine secretion was determined by using the Bio-Plex phosphoprotein and cytokines assays. Pancreatic cancer tissues and cells expressed higher amounts of T beta 10 than normal surrounding tissues and human pancreatic duct epithelial cells. Exogenous T beta 10 caused the phosphorylation of JNK and increased the secretion of cytokines interleukin (IL)-7 and IL-8 in BxPC-3 cells. T beta 10 might be a promising marker and a novel therapeutic target for pancreatic cancer.
Li
Min
M
Molecular Surgeon Research Center, Elkins Pancreas Center, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA. minli@bcm.edu
Zhang
Yuqing
Y
Zhai
Qihui
Q
Feurino
Louis W
LW
Fisher
William E
WE
Chen
Changyi
C
Yao
Qizhi
Q
D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis; D004789:Enzyme Activation; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology; D010766:Phosphorylation; D013947:Thymosin / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D016207:Cytokines; D013947:Thymosin; C039302:thymosin beta(10); D048031:JNK Mitogen-Activated Protein Kinases