title: Trypanosoma cruzi and myoid cells from seminiferous tubules: interaction and relation with fibrous components of extracellular matrix in experimental Chagas' disease.
abstract: The main transmission route of Trypanosoma cruzi is by triatomine bugs. However, T. cruzi is also transmitted through blood transfusion, organ transplantation, ingestion of contaminated food or fluids, or is congenital. Sexual transmission, although suggested since the discovery of Chagas' disease, has remained unproven. Sexual transmission would require T. cruzi to be located at the testes and ovaries. Here we investigated whether T. cruzi is present in the gonads of mice infected with 10(4) T. cruzi trypomastigotes from the CL strain. Fourteen days after experimental infection, histopathological examination showed alterations in the extracellular matrix of the lamina propria of the seminiferous tubules. Furthermore, amastigotes were present in seminiferous tubules, within myoid cells, and in the adjacencies of the basal compartment. These results indicate that T. cruzi is able to reach seminiferous tubule lumen, thus suggesting that Chagas' disease could potentially be transmitted through sexual intercourse. Complementary studies are required to demonstrate that Chagas' disease can be transmitted by coitus.
authors.affiliation: Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Luiz Otávio Pereira
authors.initials: LO
authors.lastname: Carvalho
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Abreu-Silva
authors.forename: Daiana de Jesús
authors.initials: Dde J
authors.lastname: Hardoim
authors.forename: Roberto Carlos
authors.initials: RC
authors.lastname: Tedesco
authors.forename: Verônica Gonçalves
authors.initials: VG
authors.lastname: Mendes
authors.forename: Sylvio Celso Gonçalves
authors.initials: SC
authors.lastname: da Costa
authors.forename: Kátia da Silva
authors.initials: KS
authors.lastname: Calabrese
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology* / Q000473:pathology / Q000635:transmission; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000473:pathology*; D008297:Male; D051379:Mice; D012671:Seminiferous Tubules / Q000469:parasitology* / Q000473:pathology; D013737:Testis / Q000469:parasitology / Q000473:pathology; D014349:Trypanosoma cruzi / Q000302:isolation & purification*