title: Efficacy of grape seed proanthocyanidins on cardioprotection during isoproterenol-induced myocardial injury in rats.
abstract: The present study was done to evaluate the role of grape seed proanthocyanidins (GSPs) in isoproterenol (ISO)-induced myocardial injury in rats. Male albino Wistar rats were pretreated with GSP (50, 100, and 150 mg/kg), 6 days a week, for 5 weeks. Induction of rats with ISO (85 mg/kg body weight, intraperitoneally) for 2 days resulted in a significant elevation of thiobarbituric acid-reactive substances in serum, mitochondrial cholesterol, triglycerides, and free fatty acids. A significant decrease was observed in serum reduced glutathione; ascorbic acid; alpha-tocopherol; ceruloplasmin; and mitochondrial cytochromes (b, c, c1, and aa3), phospholipids, and adenosine triphosphate. Pretreatment with GSP (100 and 150 mg/kg) positively altered the levels of all the parameters studied and restored normal mitochondrial function when compared with ISO-induced rats. The effect at a dose of 50 mg/kg was not promising when compared with the other 2 doses (100 and 150 mg/kg). These results confirm the efficacy of GSP in alleviating ISO-induced myocardial injury.
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India. karthik_research@yahoo.com
authors.forename: K
authors.initials: K
authors.lastname: Karthikeyan
authors.forename: B R Sarala
authors.initials: BR
authors.lastname: Bai
authors.forename: S Niranjali
authors.initials: SN
authors.lastname: Devaraj
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D056604:Grape Seed Extract; D007545:Isoproterenol; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D044945:Proanthocyanidins / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002316:Cardiotonic Agents; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D010936:Plant Extracts; D044945:Proanthocyanidins; D017392:Thiobarbituric Acid Reactive Substances; D007545:Isoproterenol