[Biological properties of heat-labile lethal toxin of Yersinia pseudotuberculosis].
The aim of the study was to assess the biological properties of heat-labile lethal protein toxin of Y. pseudotuberculosis. Toxin was extracted from Y. pseudotuberculosis strain 2517 type III serovar pYV-. The toxin killed mice 1-3 days after intraperitoneal administration (LD50=0.3 mcg of the protein). Heating at 56 degrees C during 30 min inactivated lethal activity of the toxin. It had a dose-dependent dermonecrotic effect during intracutaneous administration in rabbits. Hyperimmune rabbit serum to the toxin was obtained. Incubation of the toxin (LD100=1.2 mcg of the protein) with the serum at 37 degrees C during 30 min resulted in neutralization of lethal and dermonecrotic effects. The toxin did not have the cytotoxic effect on HeLa, Hep-2, and SPEV cells, but showed hemolytic activity to human and animal erythrocytes, and weak mitogenic activity to splenic cells of CBA line mice compared with control mitogen (concanavalin A).
Pokrovskiĭ
V K
VK
Timchenko
N F
NF
Nedashkovskaia
E P
EP
Grigor'eva
E A
EA
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000633:toxicity*; D002460:Cell Line; D003885:Dermotoxins / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007271:Injections, Intradermal; D007928:Lethal Dose 50; D051379:Mice; D008808:Mice, Inbred CBA; D016296:Mutagenesis; D009500:Neutralization Tests; D011817:Rabbits; D013154:Spleen / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000378:metabolism*
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D003885:Dermotoxins