Genome-wide reprogramming of regulatory networks, transport, cell wall and membrane biogenesis during arbuscular mycorrhizal symbiosis in Lotus japonicus.
* Arbuscular mycorrhizas (AMs) contribute significantly to soil nutrient uptake in plants. As a consequence of the fungal colonization and of the deep reorganization shown by arbusculated cells, important impacts on root transcriptome are expected. * An Affymetrix GeneChip with 50,000 probe-sets and real-time RT-PCR allowed us to detect transcriptional changes triggered in Lotus japonicus by the AM fungus Gigaspora margarita, when arbuscules are at their maximum (28 d postinoculation (dpi)). An early time (4 dpi) was selected to differentiate genes potentially involved in signaling and/or in colonization of outer tissues. * A large number (75 out of 558) of mycorrhiza-induced genes code for proteins involved in protein turnover, membrane dynamics and cell wall synthesis, while many others are involved in transport (47) or transcription (24). Induction of a subset (24 genes) of these was tested and confirmed by qRT-PCR, and transcript location in arbusculated cells was demonstrated for seven genes using laser-dissected cells. * When compared with previously published papers, the transcript profiles indicate the presence of a core set of responsive genes (25) that seem to be conserved irrespective of the symbiotic partner identity.
Guether
Mike
M
Department of Plant Biology, University of Torino and IPP-CNR, Viale Mattioli, 25 - 10125 Torino, Italy.
Balestrini
Raffaella
R
Department of Plant Biology, University of Torino and IPP-CNR, Viale Mattioli, 25 - 10125 Torino, Italy.
Hannah
Matthew
M
Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Postdam-Golm, Germany.
He
Ji
J
The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
Udvardi
Michael K
MK
The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
Bonfante
Paola
P
Department of Plant Biology, University of Torino and IPP-CNR, Viale Mattioli, 25 - 10125 Torino, Italy.
D001692:Biological Transport / Q000235:genetics; D002462:Cell Membrane / Q000235:genetics* / Q000382:microbiology; D002473:Cell Wall / Q000235:genetics* / Q000382:microbiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D018745:Genome, Plant / Q000235:genetics*; D000070116:Lotus / Q000166:cytology / Q000235:genetics* / Q000382:microbiology*; D042282:Microdissection; D009024:Morphogenesis / Q000235:genetics; D038821:Mycorrhizae; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013559:Symbiosis / Q000235:genetics*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D014157:Transcription Factors