The first extracellular domain of the tumour stem cell marker CD133 contains an antigenic ganglioside-binding motif.
Prominin 1/CD133 is a marker of transplantable cancer stem cells. We have generated anti-peptide antibodies against a N-terminal epitope of CD133 belonging to a ganglioside-binding domain. The labelling of colon cancer cells with these antibodies was inhibited by various gangliosides including GM1 and GD3, but not GT1b. CD133 immunolabelling progressively decreased to undetectable levels in post-confluent cultures, possibly through ganglioside-mediated epitope masking since the staining was partially recovered after chemical disruption of lipid rafts. We suggest that selected gangliosides could modulate the accessibility of CD133 and regulate cell-cell contacts involving CD133(+) stem cells at the earliest steps of tumour development.
Taïeb
Nadira
N
CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
Maresca
Marc
M
CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
Guo
Xiao-Jun
XJ
CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
Garmy
Nicolas
N
CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
Fantini
Jacques
J
CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
Yahi
Nouara
N
CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France. Electronic address: nouara.yahi@univ-cezanne.fr.
D000071916:AC133 Antigen; D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000032:analysis; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D021962:Membrane Microdomains / Q000502:physiology; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary
D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D005732:Gangliosides; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D005677:G(M1) Ganglioside; C026226:ganglioside, GD3