Zavarzinella formosa gen. nov., sp. nov., a novel stalked, Gemmata-like planctomycete from a Siberian peat bog.
An aerobic, pink-pigmented, budding and rosette-forming bacterium was isolated from an acidic Sphagnum peat bog and designated strain A10(T). The 16S rRNA gene sequence analysis showed that strain A10(T) was a member of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Gemmata, with 90 % sequence similarity to that of Gemmata obscuriglobus, the only taxonomically described organism of this group. Ellipsoid-shaped cells of strain A10(T) were uniformly covered with crateriform pits and possessed long (up to 10-15 mum) and unusually thick (0.5-0.7 mum) stalks of a unique ultrastructure. Thin sections revealed a complex intracellular membrane system compartmentalizing the cells. Strain A10(T) was a moderately acidophilic, mesophilic organism capable of growth at pH values between 3.8 and 7.2 (with an optimum at pH 5.5-6.0) and at temperatures between 10 and 30 degrees C (with an optimum at 20-25 degrees C). The major fatty acids were C(18 : 0), C(18 : 1)omega5c and C(16 : 1)omega5c and the major quinone was MK-6. Cells of strain A10(T) contained high amounts of bound saturated and monounsaturated C(26)-C(32) (omega-1) hydroxy fatty acids. The G+C content of the DNA was 62.5 mol%. The unique cell morphology, the capability of growth in acidic conditions and a number of chemotaxonomic and genotypic characteristics served to differentiate strain A10(T) from G. obscuriglobus. Based on these data, the novel isolate should be considered as representing a novel genus and species of planctomycetes, for which the name Zavarzinella formosa gen. nov., sp. nov. is proposed The type strain is A10(T) (=DSM 19928(T)=VKM B-2478(T)).
Kulichevskaya
Irina S
IS
S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
Baulina
Olga I
OI
Bodelier
Paul L E
PL
Rijpstra
W Irene C
WI
Damsté
Jaap S Sinninghe
JS
Dedysh
Svetlana N
SN
D001419:Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012800:Siberia; D012987:Soil; D012988:Soil Microbiology; D013045:Species Specificity
D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D012987:Soil