Methods for the recovery of a model virus from healthcare personal protective equipment.
OBJECTIVE
To develop methods for recovering a model virus (bacteriophage MS2) from healthcare personal protective equipment (PPE).


RESULTS
Nine eluents were evaluated for recovery of infectious MS2 from PPE: 1.5% beef extract (BE) pH 7.5 with and without 0.1% Tween 80, 1.5% BE pH 9.0 with and without 0.1% Tween 80, 3% BE pH 7.5 with and without 0.1% Tween 80, 3% BE pH 9.0 with and without 0.1% Tween 80 and PBS with 0.1% Tween 80. Methods were applied to experimentally contaminated PPE. Elution followed by two-step enrichment assay could recover virus inputs as low as 1.5 log(10), and could recover >90% of inoculated virus from used items of experimentally contaminated PPE worn by human volunteers.


CONCLUSIONS
BE was effective for recovering infectious viruses from a range of PPE materials.


CONCLUSIONS
PPE plays a crucial role in interrupting transmission of infectious agents from patients to healthcare workers (HCWs). The fate of micro-organisms when PPE is removed and disposed of has important consequences for infection control. Methods described here can be used to conduct rigorous studies of viral survival and transfer on PPE for risk assessments in infection control and HCW protection.
Casanova
L
L
Department of Environmental Sciences and Engineering, University of North Carolina Chapel Hill, 27599, USA.
Rutala
W A
WA
Weber
D J
DJ
Sobsey
M D
MD
D006282:Health Personnel; D006801:Humans; D011482:Protective Devices / Q000821:virology*; D010948:Viral Plaque Assay / Q000379:methods*; D014777:Virus Diseases / Q000517:prevention & control; D014780:Viruses / Q000254:growth & development / Q000302:isolation & purification*