Genomic epidemiology of a dengue virus epidemic in urban Singapore.
Dengue is one of the most important emerging diseases of humans, with no preventative vaccines or antiviral cures available at present. Although one-third of the world's population live at risk of infection, little is known about the pattern and dynamics of dengue virus (DENV) within outbreak situations. By exploiting genomic data from an intensively studied major outbreak, we are able to describe the molecular epidemiology of DENV at a uniquely fine-scaled temporal and spatial resolution. Two DENV serotypes (DENV-1 and DENV-3), and multiple component genotypes, spread concurrently and with similar epidemiological and evolutionary profiles during the initial outbreak phase of a major dengue epidemic that took place in Singapore during 2005. Although DENV-1 and DENV-3 differed in viremia and clinical outcome, there was no evidence for adaptive evolution before, during, or after the outbreak, indicating that ecological or immunological rather than virological factors were the key determinants of epidemic dynamics.
Schreiber
Mark J
MJ
Novartis Institute for Tropical Diseases, Chromos, Singapore. mark.schreiber@novartis.com
Holmes
Edward C
EC
Ong
Swee Hoe
SH
Soh
Harold S H
HS
Liu
Wei
W
Tanner
Lukas
L
Aw
Pauline P K
PP
Tan
Hwee Cheng
HC
Ng
Lee Ching
LC
Leo
Yee Sin
YS
Low
Jenny G H
JG
Ong
Adrian
A
Ooi
Eng Eong
EE
Vasudevan
Subhash G
SG
Hibberd
Martin L
ML
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D009033:Culicidae; D003715:Dengue / Q000097:blood / Q000453:epidemiology*; D003716:Dengue Virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics; D023281:Genomics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012846:Singapore / Q000453:epidemiology; D014504:Urban Health / Q000706:statistics & numerical data*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins