title: Coumarin-derived Cu(2+)-selective fluorescence sensor: synthesis, mechanisms, and applications in living cells.
abstract: A novel coumarin-based fluorogenic probe bearing the 2-picolyl unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu(2+) over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu(2+) ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu(2+) in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu(2+).
authors.affiliation: Department of Chemistry, Korea University, Seoul 136-701, Korea.
authors.forename: Hyo Sung
authors.initials: HS
authors.lastname: Jung
authors.forename: Pil Seung
authors.initials: PS
authors.lastname: Kwon
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Chang Seop
authors.initials: CS
authors.lastname: Hong
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Shihai
authors.initials: S
authors.lastname: Yan
authors.forename: Jin Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jung Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Taiha
authors.initials: T
authors.lastname: Joo
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002413:Cations, Divalent; D002478:Cells, Cultured; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008253:Macaca mulatta; D008856:Microscopy, Fluorescence / Q000379:methods; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002413:Cations, Divalent; D003374:Coumarins; D005456:Fluorescent Dyes; D003300:Copper