Hb Belleville [beta 10(A7)Ala-->Thr] affects the determination of Hb A1c by routine cation exchange high performance liquid chromatography.
When analyzed by routine cation exchange high performance liquid chromatography (HPLC), the Hb A(1c) peak of a Caucasian diabetic patient from Yorkshire, UK, appeared to be an incompletely resolved doublet. One component (5.5%) eluted at the normal time for Hb A(1c), whereas the other component (6.6%) eluted slightly later. The HPLC trace was otherwise normal. Analysis of the diabetic patient's blood and a tryptic digest thereof by electrospray ionization mass spectrometry (ESI-MS) identified the Hb Belleville trait. To relate Hb A(1c) determined by HPLC to alpha and beta chain glycation determined by ESI-MS, several normal blood samples (5-14% Hb A(1c)) were analyzed by both techniques. The Hb A(1c) levels derived from the alpha and beta chain glycation levels of the diabetic patient (12.9 and 12.4%, respectively) agreed with the sum of the two peaks (12.1%) in the HPLC trace. Similarly, Hb Belleville was detected and identified in another Caucasian, also from Yorkshire, with normal Hb A(1c).
Williams
Jonathan P
JP
Department of Chemistry, University of Warwick, Coventry, Warwickshire, UK. J.P.Williams@warwick.ac.uk
Jackson
Hannah
H
Green
Brian N
BN
D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003920:Diabetes Mellitus; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D021241:Spectrometry, Mass, Electrospray Ionization; D044465:White People; D055815:Young Adult
D002411:Cation Exchange Resins; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C540074:hemoglobin Belleville