Impaired uptake of beta-carotene by Caco-2 human intestinal cells in the presence of iron.
At present, there are conflicting data regarding whether or not beta-carotene has a positive effect on iron absorption. This study was undertaken to evaluate possible interactions involved in the uptake of beta-carotene and iron in a human intestinal cell model (Caco-2). The Caco-2 cells were incubated with test solutions containing different amounts of ferrous chloride (10-50 microM) and beta-carotene (0.3-2.5 microM) incorporated in synthetic micelles. In the absence of iron, cellular accumulation of beta-carotene from synthetic micelles was proportional (r (2)=0.97, P <0.001) to the beta-carotene concentration in the test solution. However, with addition of ferrous chloride (30 microM), the beta-carotene uptake was significantly reduced (P <0.05), on average by 22%. There was also an inverse relationship between the beta-carotene uptake and iron concentration in the test solution (r (2)=0.93, P <0.05). Iron provided in physiological amounts inhibited the uptake of beta-carotene in the in vitro Caco-2 cell system.
Bengtsson
Anton
A
Department of Chemical and Biological Engineering, Food Science, Chalmers University of Technology, Goteborg, Sweden. anton.bengtsson@chalmers.se
Scheers
Nathalie
N
Andlid
Thomas
T
Alminger
Marie Larsson
ML
Sandberg
Ann-Sofie
AS
Svanberg
Ulf
U
D001692:Biological Transport; D018938:Caco-2 Cells; D020895:Enterocytes / Q000378:metabolism*; D005293:Ferritins / Q000378:metabolism; D005296:Ferrous Compounds; D006801:Humans; D007408:Intestinal Absorption; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D008823:Micelles; D009994:Osmolar Concentration; D019207:beta Carotene / Q000302:isolation & purification / Q000378:metabolism*
D005296:Ferrous Compounds; D008823:Micelles; D019207:beta Carotene; D005293:Ferritins; D007501:Iron; C029451:ferrous chloride