title: Characterization and application of a new monoclonal antibody with high specificity for Helicobacter hepaticus.
abstract: OBJECTIVE
  Infection with Helicobacter hepaticus is suggested to play a role in the pathogenesis of chronic liver disease in humans. However, reactive antigens among Helicobacter species make the development of an H. hepaticus ELISA test with high specificity difficult. A new monoclonal antibody from a hybridoma clone (HRII-51) showed high specificity to H. hepaticus without cross-reaction to other gastrointestinal bacteria.
  
  
  METHODS
  The molecular weight of HRII-51 immunoreactive antigen was examined by Western blot of H. hepaticus probed with the monoclonal antibody HRII-51. A HRII-51-immunoreactive antigen capture ELISA was prepared in which the specific antigen was anchored by HRII-51-immobilized ELISA plate. Accuracy of HRII-51 antigen capture ELISA was examined using sera obtained from mice inoculated with Helicobacter species. Specificity of HRII-51 antigen capture ELISA was compared to that of H. hepaticus antigen-based ELISA using human sera with absorption by H. pylori cell lysate.
  
  
  RESULTS
  HRII-51 immunoreactive antigen had a molecular weight of 15 kDa. Sensitivity and specificity of HRII-51 antigen capture ELISA were 87.0% and 97.6% in mice inoculated with Helicobacter species. In human sera, modification of the results by absorption with H. pylori lysate was smaller in HRII-51 antigen capture ELISA comparing with H. hepaticus-antigen-based ELISA.
  
  
  CONCLUSIONS
  Use of the HRII-51 antigen capture ELISA would be a useful approach for the serodiagnosis of H. hepaticus infection in both experimental animals and humans.
authors.affiliation: Hyōgo College of Medicine, Nishinomiya, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shimoyama
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohmura
authors.forename: Yukari
authors.initials: Y
authors.lastname: Sano
authors.forename: Natsuko
authors.initials: N
authors.lastname: Nakabayashi
authors.forename: Ryoki
authors.initials: R
authors.lastname: Takahashi
authors.forename: Toshio
authors.initials: T
authors.lastname: Fujioka
authors.forename: Hazel M
authors.initials: HM
authors.lastname: Mitchell
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimoyama
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D044923:Helicobacter hepaticus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal