Monitoring biomolecular interactions on a digital versatile disk: a BioDVD platform technology.
A spinning-disk biosensor utilizing optical interference of reflected light from a multilayered structure, consisting of dielectric, metal, and optical phase-change thin films, is shown to have the potential to monitor various interactions on its surface. We refer to this platform as a BioDVD, since it utilizes the optical system of a digital versatile disk (DVD) to measure changes in reflected light intensity. Here, we demonstrated that nucleic acid hybridization and RNA-protein interactions can be analyzed efficiently, in a label-free environment, by measuring the reflected light intensity using a DVD-like mechanism. Moreover, our studies revealed that the detection sensitivity for the interactions on the BioDVD can be altered by shifting the state of the phase-change materials, where the amorphous state can be used for analysis and another state (crystalline) can be used both for recording information and selectively masking areas of the disk.
Gopinath
Subash C B
SC
Center for Applied Near Field Optics Research, Central 4, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, 305-8562, Japan.
Awazu
Koichi
K
Tominaga
Junji
J
Kumar
Penmetcha K R
PK
D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D007834:Lasers; D036103:Nanotechnology / Q000379:methods*
D001704:Biopolymers