title: [Diagnostic value of leukocytic alteration test (in vitro) for drug-induced allergy in a therapist's practice].
abstract: The laboratory diagnostic process of drug intolerance is a clinical challenge. The allergic leukocytic alteration test (ALAT) was used to detect drug intolerance and to estimate its informative value. Three hundred and eighty-seven patients with various manifestations of drug allergy were examined (1227 studies). The test was carried out in vitro using heparinized blood, calculated with reference with 1 ml per drug and incubated at 37 degrees C for 1.5 hours. Smears were prepared, stained by the Romanovsky-Giemsa method, and examined by light microscopy. A hundred cells (neutrophils) were scanned to evaluate allergic alteration. When the affected neutrophils were more than 10%, the test was considered as positive. The findings suggest that ALAT reveals intolerance of different groups of drugs with a high degree of significance and may be proposed for the diagnosis of drug allergy as it is of great informative value, safe to a patient, and allows a large number of drugs to be tested.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Khalimova
authors.forename: E M
authors.initials: EM
authors.lastname: Esedov
authors.forename: L B
authors.initials: LB
authors.lastname: Ashurbekova
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004342:Drug Hypersensitivity / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D006403:Hematologic Tests; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests