title: The cerebellin 4 precursor gene is a direct target of SRY and SOX9 in mice.
abstract: In most mammals, the expression of SRY (sex-determining region on the Y chromosome) initiates the development of testes, and thus determines the sex of the individual. However, despite the pivotal role of SRY, its mechanism of action remains elusive. One important missing piece of the puzzle is the identification of genes regulated by SRY. In this study we used chromatin immunoprecipitation to identify direct SRY target genes. Anti-mouse SRY antibody precipitated a region 7.5 kb upstream of the transcriptional start site of cerebellin 4 precursor (Cbln4), which encodes a secreted protein. Cbln4 is expressed in Sertoli cells in the developing gonad, with a profile mimicking that of the testis-determining gene SRY-box containing gene 9 (Sox9). In transgenic XY mouse embryos with reduced Sox9 expression, Cbln4 expression also was reduced, whereas overexpression of Sox9 in XX mice caused an upregulation of Cbln4 expression. Finally, ectopic upregulation of SRY in vivo resulted in ectopic expression of Cbln4. Our findings suggest that both SRY and SOX9 contribute to the male-specific upregulation of Cbln4 in the developing testis, and they identified a direct in vivo target gene of SRY.
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, Queensland, Australia.
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Bradford
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hiramatsu
authors.forename: Madhavi P
authors.initials: MP
authors.lastname: Maddugoda
authors.forename: Pascal
authors.initials: P
authors.lastname: Bernard
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Chaboissier
authors.forename: Andrew
authors.initials: A
authors.lastname: Sinclair
authors.forename: Andreas
authors.initials: A
authors.lastname: Schedl
authors.forename: Vincent
authors.initials: V
authors.lastname: Harley
authors.forename: Yoshiakira
authors.initials: Y
authors.lastname: Kanai
authors.forename: Peter
authors.initials: P
authors.lastname: Koopman
authors.forename: Dagmar
authors.initials: D
authors.lastname: Wilhelm
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D011498:Protein Precursors / Q000502:physiology*; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D055758:SOX9 Transcription Factor; D051876:Sex-Determining Region Y Protein; C527933:Sox9 protein, mouse; C497746:Sry protein, mouse; C542343:cerebellin 4 precursor, mouse; C046788:cerebellin