title: Monitoring biomolecular interactions on a digital versatile disk: a BioDVD platform technology.
abstract: A spinning-disk biosensor utilizing optical interference of reflected light from a multilayered structure, consisting of dielectric, metal, and optical phase-change thin films, is shown to have the potential to monitor various interactions on its surface. We refer to this platform as a BioDVD, since it utilizes the optical system of a digital versatile disk (DVD) to measure changes in reflected light intensity. Here, we demonstrated that nucleic acid hybridization and RNA-protein interactions can be analyzed efficiently, in a label-free environment, by measuring the reflected light intensity using a DVD-like mechanism. Moreover, our studies revealed that the detection sensitivity for the interactions on the BioDVD can be altered by shifting the state of the phase-change materials, where the amorphous state can be used for analysis and another state (crystalline) can be used both for recording information and selectively masking areas of the disk.
authors.affiliation: Center for Applied Near Field Optics Research, Central 4, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, 305-8562, Japan.
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.forename: Koichi
authors.initials: K
authors.lastname: Awazu
authors.forename: Junji
authors.initials: J
authors.lastname: Tominaga
authors.forename: Penmetcha K R
authors.initials: PK
authors.lastname: Kumar
mesh_terms: D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D007834:Lasers; D036103:Nanotechnology / Q000379:methods*
chemical_list: D001704:Biopolymers