title: One-step production of immobilized alpha-amylase in recombinant Escherichia coli.
abstract: Industrial enzymes are often immobilized via chemical cross-linking onto solid supports to enhance stability and facilitate repeated use in bioreactors. For starch-degrading enzymes, immobilization usually places constraints on enzymatic conversion due to the limited diffusion of the macromolecular substrate through available supports. This study describes the one-step immobilization of a highly thermostable alpha-amylase (BLA) from Bacillus licheniformis and its functional display on the surface of polyester beads inside engineered Escherichia coli. An optimized BLA variant (Termamyl) was N-terminally fused to the polyester granule-forming enzyme PhaC of Cupriavidus necator. The fusion protein lacking the signal sequence mediated formation of stable polyester beads exhibiting alpha-amylase activity. The alpha-amylase beads were assessed with respect to alpha-amylase activity, which was demonstrated qualitatively and quantitatively. The immobilized alpha-amylase showed Michaelis-Menten enzyme kinetics exerting a V(max) of about 506 mU/mg of bead protein with a K(m) of about 5 microM, consistent with that of free alpha-amylase. The stability of the enzyme at 85 degrees C and the capacity for repeated usage in a starch liquefaction process were also demonstrated. In addition, structural integrity and functionality of the beads at extremes of pH and temperature, demonstrating their suitability for industrial use, were confirmed by electron microscopy and protein/enzyme analysis. This study proposes a novel, cost-effective method for the production of immobilized alpha-amylase in a single step by using the polyester granules forming protein PhaC as a fusion partner in engineered E. coli.
authors.affiliation: Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
authors.forename: Indira A
authors.initials: IA
authors.lastname: Rasiah
authors.forename: Bernd H A
authors.initials: BH
authors.lastname: Rehm
mesh_terms: D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D011091:Polyesters / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013696:Temperature; D000516:alpha-Amylases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D011091:Polyesters; D011993:Recombinant Fusion Proteins; D013213:Starch; D000516:alpha-Amylases