title: Hydrophilic fluorescent nanogel thermometer for intracellular thermometry.
abstract: The first methodology to measure intracellular temperature is described. A highly hydrophilic fluorescent nanogel thermometer developed for this purpose stays in the cytoplasm and emits stronger fluorescence at a higher temperature. Thus, intracellular temperature variations associated with biological processes can be monitored by this novel thermometer with a temperature resolution of better than 0.5 degrees C.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Gota
authors.forename: Kohki
authors.initials: K
authors.lastname: Okabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Harada
authors.forename: Seiichi
authors.initials: S
authors.lastname: Uchiyama
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D013821:Thermometers
chemical_list: D000180:Acrylic Resins; D005456:Fluorescent Dyes; C052970:poly-N-isopropylacrylamide