Parainfluenza virus type 5 (PIV-5) morphology revealed by cryo-electron microscopy.
The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology.
Terrier
Olivier
O
Université de Lyon, F-69000 Lyon, France. olivier.terrier@sante.univ-lyon1.fr
Rolland
Jean-Paul
JP
Rosa-Calatrava
Manuel
M
Lina
Bruno
B
Thomas
Daniel
D
Moules
Vincent
V
D020285:Cryoelectron Microscopy; D018098:Paramyxovirinae / Q000737:chemistry / Q000648:ultrastructure*; D010316:Particle Size; D014759:Viral Envelope Proteins / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure*
D014759:Viral Envelope Proteins