Effect of sesame oil on acidified ethanol-induced gastric mucosal injury in rats.
BACKGROUND
Exposure of gastric mucosa to concentrated ethanol induces acute gastritis. Gastric mucosal lipid peroxidation plays a significant role in the pathogenesis of ethanol-induced gastric mucosal lesions. The aim of this study was to investigate the effect of sesame oil on acidified ethanol-induced gastric mucosal damage in rats.


METHODS
We performed gastric bilateral vagotomy in rats. A small incision on forestomach was made and stomach content was expelled. Normal artificial gastric acid (54 mM NaCl plus 100 mM HCl) or acidified ethanol (30% ethanol plus 150 mM HCl) was instilled into the stomach. Gastric lipid peroxidation, glutathione, and nitric oxide levels were measured 3 hours after acidified ethanol administration.


RESULTS
Acidified ethanol caused mucosal ulceration, luminal hemorrhage, lipid peroxidation, and a lower level of mucosal glutathione and nitric oxide production. Pretreatment of sesame oil, but not mineral oil, significantly decreased acidified ethanol-induced mucosal ulcer formation and luminal hemorrhage. Sesame oil reduced mucosal lipid peroxidation, as well as glutathione and nitric oxide production in acidified ethanol-treated stomachs. Furthermore, both sesame oil and mineral oil did not affect serum ethanol concentration in acidified ethanol-treated rats.


CONCLUSIONS
Sesame oil attenuates acidified ethanol-induced gastric mucosal injury by reducing oxidative stress in rats.
Hsu
Dur-Zong
DZ
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan.
Chu
Pei-Yi
PY
Liu
Ming-Yie
MY
D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects* / Q000097:blood; D005744:Gastric Acid; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005756:Gastritis / Q000517:prevention & control*; D005978:Glutathione / Q000378:metabolism; D006470:Hemorrhage / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008899:Mineral Oil / Q000627:therapeutic use; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012715:Sesame Oil / Q000494:pharmacology*; D013276:Stomach Ulcer / Q000517:prevention & control
D009569:Nitric Oxide; D000431:Ethanol; D012715:Sesame Oil; D008899:Mineral Oil; D005978:Glutathione