title: Bone regeneration with autologous plasma, bone marrow stromal cells, and porous beta-tricalcium phosphate in nonhuman primates.
abstract: To potentiate the bone formation capability of bone marrow stromal cell (BMSC)/beta-tricalcium phosphate (beta-TCP) constructs, we devised an autologous plasma-based construct. We tested its effectiveness and investigated the effects of its components on a monkey ectopic bone formation model. The autologous plasma (platelet-rich plasma, PRP, or platelet-poor plasma, PPP)/BMSC/beta-TCP construct (R group or P group) showed significantly more bone formation at 3 and 6 weeks after implantation than a conventional BMSC/beta-TCP construct using a culture medium (M group). There was no significant difference between the P and R groups. Moreover, the P group constructs with a 10-fold lower cell concentration yielded equivalent bone formation to the M group at 5 weeks after implantation. To elucidate the effect of fibrin and serum contained in the plasma, five constructs were prepared using the following cell vehicles: autologous serum + fibrinogen (0, 1, 4, or 16 mg/mL) or phosphate-buffered saline + fibrinogen (4 mg/mL). The serum + fibrinogen (4 mg/mL, physiological concentration of monkeys) construct showed the most abundant bone formation at 3 weeks after implantation, though at 5 weeks no statistical difference existed among the groups. Autologous plasma efficiently promoted osteogenesis of BMSCs/porous beta-TCP constructs, and both fibrin and serum proved to play significant roles in the mechanism.
authors.affiliation: Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Torigoe
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sotome
authors.forename: Akio
authors.initials: A
authors.lastname: Tsuchiya
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Yoshii
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Maehara
authors.forename: Yumi
authors.initials: Y
authors.lastname: Sugata
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shinomiya
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okawa
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology*; D003470:Culture Media; D005337:Fibrin / Q000494:pharmacology; D006801:Humans; D008252:Macaca fascicularis / Q000502:physiology*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D010949:Plasma / Q000378:metabolism*; D016062:Porosity / Q000187:drug effects; D019919:Prosthesis Implantation; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D002130:Calcium Phosphates; D003470:Culture Media; C485817:beta-tricalcium phosphate; D005337:Fibrin