Characterization of the cell surface glycolipid from Spirochaeta aurantia.
Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D: -manno-heptose (DDHep), and a novel branched tetradeoxydecose monosaccharide, which we proposed to call aurantose (Aur). The carbohydrate structure of LGLA was extremely complex and consisted of the repeating units built of 11 monosaccharides, arrangement of nine of them was determined as: - [- 3 - beta - DDglcHep - 3 - beta - D - GlcNAc - 2 - beta - D - Man - ] - which wasdeduced from the NMR and chemical data on the LGLA and its fragments, obtained by various degradations. Tentative position of two remaining sugars is proposed. LGLA was negative for gelation of Limulus amebocyte lysate, did not contain lipid A, and was unable to activate any known Toll-like receptors.
Paul
Catherine J
CJ
Department of Biotechnology, Lund University, Lund, Sweden, 22100.
Lyle
Elizabeth A
EA
Beveridge
Terry J
TJ
Tapping
Richard I
RI
Kropinski
Andrew M
AM
Vinogradov
Evgeny
E
D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013142:Spirochaeta / Q000378:metabolism* / Q000648:ultrastructure; D051193:Toll-Like Receptors / Q000378:metabolism
D006017:Glycolipids; D051193:Toll-Like Receptors