Expansion and characterization of human bone marrow-derived mesenchymal stem cells cultured on fragmin/protamine microparticle-coated matrix with fibroblast growth factor-2 in low serum medium.
Fragmin/protamine microparticles (F/P MPs) can be stably coated onto plastic surfaces. A capability of F/P MP-coated plates was investigated to immobilize fibroblast growth factor (FGF)-2 as a substratum to expand human bone marrow-derived mesenchymal stem cells (BMMSCs). FGF-2 molecules in low (2%) human serum (HS) medium were immobilized onto F/P MP-coated plates, and the FGF-2 was gradually released into the medium with a half-releasing time of 4-5 days. BMMSCs adhered well to the F/P MP-coated plates, and grew at a doubling time of about 28 h in low (2%) HS medium with FGF-2 (5 ng/mL), while the cells grew at a doubling time of about 30 and 38 h in high (10%) HS medium and in low (2%) HS medium with FGF-2, respectively, without F/P MP coating. The expanded BMMSCs on the F/P MP-coated plates in low (2%) HS medium with FGF-2 maintained their multilineage potential for differentiation into adipocytes and osteoblasts.
Kishimoto
Satoko
S
Research Institute, National Defense Medical College, Saitama 359-8513, Japan.
Hattori
Hidemi
H
Nakamura
Shingo
S
Amano
Yoshiko
Y
Kanatani
Yasuhiro
Y
Tanaka
Yoshihiro
Y
Mori
Yasutaka
Y
Harada
Yasuji
Y
Tagawa
Masahiro
M
Ishihara
Masayuki
M
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017985:Dalteparin / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D011479:Protamines / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D020099:Coated Materials, Biocompatible; D011479:Protamines; D016222:Fibroblast Growth Factor 2; D017985:Dalteparin