Functionalization of luminescent aminated particles for facile bioconjugation.
For labeling proteins (streptavidin and fibronectin) by luminescent aminated nanoparticles, an interesting strategy that requires neither activation nor chemical pre- or post-treatment was explored. Because biomolecules are easily rendered luminescent after reaction with organic dyes carrying isothiocyanate moiety, phenylene diisothiocyanate (DITC) was used for covalently binding proteins onto luminescent hybrid gadolinium oxide nanoparticles whose ability to combine imaging and therapy was recently demonstrated.
Faure
Anne-Charlotte
AC
Laboratoire de Physico-Chimie des Materiaux Luminescents, UMR 5620 CNRS-Universite Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France.
Hoffmann
Céline
C
Bazzi
Rana
R
Goubard
Fabrice
F
Pauthe
Emmanuel
E
Marquette
Christophe A
CA
Blum
Loïc J
LJ
Perriat
Pascal
P
Roux
Stéphane
S
Tillement
Olivier
O
D001710:Biotin / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D003460:Crystallization; D005353:Fibronectins / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods; D011522:Protons; D012833:Siloxanes / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
D004396:Coloring Agents; D005353:Fibronectins; D011522:Protons; D012833:Siloxanes; C030581:gadolinium oxide; D001710:Biotin; D019809:Streptavidin; D005682:Gadolinium