Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria.
The nanoscale exploration of microbes using atomic force microscopy (AFM) is an exciting, rapidly evolving research field. Here, we show that single-molecule force spectroscopy is a valuable tool for the localization and conformational analysis of individual polysaccharides on live bacteria. We focus on the clinically important probiotic bacterium Lactobacillus rhamnosus GG, demonstrating the power of AFM to reveal the coexistence of polysaccharide chains of different nature on the cell surface. Applicable to a wide variety of cells, this single molecule method offers exciting prospects for analyzing the heterogeneity and diversity of macromolecules constituting cell membranes and cell walls.
Francius
Grégory
G
Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2/18, B-1348 Louvain-la-Neuve, Belgium.
Lebeer
Sarah
S
Alsteens
David
D
Wildling
Linda
L
Gruber
Hermann J
HJ
Hols
Pascal
P
De Keersmaecker
Sigrid
S
Vanderleyden
Jos
J
Dufrêne
Yves F
YF
D007089:Image Enhancement / Q000379:methods*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism* / Q000648:ultrastructure*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008968:Molecular Conformation
D008070:Lipopolysaccharides