Identification of a novel common proviral integration site, flit-1, in feline leukemia virus induced thymic lymphoma.
A new proviral integration site for feline leukemia virus (FeLV), termed flit-1, was identified from feline thymic lymphoma. Among 35 FeLV-related tumors examined, 5 of 25 thymic lymphomas demonstrated proviral insertion within flit-1 locus whereas none of four alimentary and five multicentric lymphomas and one T-lymphoid leukemia examined had rearrangement in this region. Extensive sequence analysis has shown that flit-1, which is noncoding, is conserved on human chromosome 12 and mouse chromosome 15. The human and murine homologs of flit-1 are positioned approximately 30-kb upstream to activin-A receptor type II-like 1 (ACVRL1/ALK1) gene. Expression of ACVRL1 mRNA was examined in two of five lymphomas with flit-1 rearrangement and detected in both of the two whereas normal thymuses and seven lymphoid tumors without flit-1 rearrangement had no detectable expression. Therefore, flit-1 appears to represent a novel FeLV proviral common integration domain that may influence lymphomagenesis as insertional mutagenesis.
Fujino
Yasuhito
Y
Department of Pathology, University of Southern California School of Medicine, Los Angeles, CA 90033, USA. afujino@mail.ecc.u-tokyo.ac.jp
Liao
Chun-Peng
CP
Zhao
Yan Shi
YS
Pan
Judong
J
Mathes
Lawrence E
LE
Hayes
Kathleen A
KA
Ohno
Koichi
K
Tsujimoto
Hajime
H
Roy-Burman
Pradip
P
D000818:Animals; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D023061:Gene Order; D015321:Gene Rearrangement; D005256:Leukemia Virus, Feline / Q000502:physiology*; D008223:Lymphoma / Q000473:pathology / Q000821:virology*; D011533:Proviruses / Q000502:physiology*; D013950:Thymus Gland / Q000473:pathology / Q000821:virology*; D016662:Virus Integration