Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues.
OBJECTIVE
The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues.


METHODS
Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation.


RESULTS
The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.


CONCLUSIONS
Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.
Zhang
Jian-Min
JM
Department of Obstetrics and Gynecology, First People's Hospital Affiliated to Shanghai Jiao Tong University, Haining Road 100, Shanghai 200080, China.
Li
Lin-Xia
LX
Liu
Xue-Lian
XL
Yang
Yi-Xia
YX
Wan
Xiao-Ping
XP
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000502:physiology; D010053:Ovary / Q000637:transplantation*; D027724:Reproductive Techniques, Assisted; D013395:Sucrose
D003451:Cryoprotective Agents; D004958:Estradiol; D013395:Sucrose