A common gene exclusion mechanism used by two chemosensory systems.
Sensory coding strategies within vertebrates involve the expression of a limited number of receptor types per sensory cell. In mice, each vomeronasal sensory neuron transcribes monoallelically a single V1R pheromone receptor gene, chosen from a large V1R repertoire. The nature of the signals leading to this strict receptor expression is unknown, but is apparently based on a negative feedback mechanism initiated by the transcription of the first randomly chosen functional V1R gene. We show, in vivo, that the genetic replacement of the V1rb2 pheromone receptor coding sequence by an unrelated one from the odorant receptor gene M71 maintains gene exclusion. The expression of this exogenous odorant receptor in vomeronasal neurons does not trigger the transcription of odorant receptor-associated signalling molecules. These results strongly suggest that despite the different odorant and vomeronasal receptor expression sites, function and transduction cascades, a common mechanism is used by these chemoreceptors to regulate their transcription.
Capello
Luca
L
Department of Zoology and Animal Biology, and NCCR Frontiers in Genetics, University of Geneva, Geneva, Switzerland.
Roppolo
Daniele
D
Jungo
Véronique Pauli
VP
Feinstein
Paul
P
Rodriguez
Ivan
I
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009830:Olfactory Bulb / Q000378:metabolism; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism; D043563:Receptors, Pheromone / Q000235:genetics* / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism*; D017385:Sequence Homology; D019147:Vomeronasal Organ / Q000378:metabolism*
C502656:Olfr151 protein, mouse; D018035:Receptors, Odorant; D043563:Receptors, Pheromone; C529159:V1rb2 protein, mouse