title: [Expression of mammalian target of rapamycin and p70 S6 kinase in rabbit periodontal tissues remodeling during orthodontic tooth movement].
abstract: OBJECTIVE
  To study the role of mammalian target of rapamycin (mTOR) and p70 S6 kinase (p70 S6K) in periodontal tissues remodeling during orthodontic tooth movement.
  
  
  METHODS
  Twenty-four rabbits were chosen to establish rabbit models for the study. The right maxillary teeth of each animal treated by orthodontics were the test sides, and the untreated left teeth were the control sides. The animals were sacrificed at 3, 5, 7, 14 d, respectively. The prepared tissue specimens were processed for the study of the change in the histologic morphology with HE staining and the expression of p70 S6K in periodontal tissues by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot techniques.
  
  
  RESULTS
  In the test side, the tranecula of bone became porous and cells arrayed disorderly, resorption of alveolar bone was observed in histological study. There were many holes in the alveolar bone, osteoblasts were occasionally observed. RT-PCR showed that the expression of p70 S6K mRNA dramatically changed at 3 d. The expression of p70 S6K mRNA in treating periodontal tissues was higher than the control side, especially at 7 d, and then decreased. Compared with the control side, there was significant difference in statistical analysis (P<0.01). The expression of Western blot was same as the RT-PCR.
  
  
  CONCLUSIONS
  Higher expression of mTOR and p70 S6K during orthodontic tooth movement especially plays an important role in the process of periodontium remodeling.
authors.affiliation: Dept. of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Ya-feng
authors.initials: YF
authors.lastname: Guo
mesh_terms: D000818:Animals; D010006:Osteoblasts; D010513:Periodontal Ligament; D011817:Rabbits; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D020123:Sirolimus; D058570:TOR Serine-Threonine Kinases; D014087:Tooth Movement Techniques
chemical_list: C546842:MTOR protein, human; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus