[Inhibiting effect of complex of natural cytokines and cationic antimicrobial peptides on the growth of Mycobacterium tuberculosis H37Rv in vitro].
OBJECTIVE
To assess direct antimicrobial effect of complex of natural cytokines (CNC) and antimicrobial peptides (Syperlymph preparation; CNC) on Mycobacterium tuberculosis H37Rv (H37Rv) and effect mediated by macrophages (MP) treated with the preparation.


METHODS
Direct effect of CNC was studied during cultivation of H37Rv in the presence of preparation, whereas indirect effects--during simultaneous cultivation of H37Rv and mice peritoneal MP C57B1/6. Assessment of growth was performed on the 7th day using PCR.


RESULTS
It was shown that CNC directly inhibits growth of H37Rv in vitro. Cultivation of H37Rv in culture of MP resulted in inhibition of M. tuberculosis. The most evident inhibition was noted after extension of time of preliminary treatment of MP with Syperlymph and simultaneous increase of its concentration.


CONCLUSIONS
Antimycobacterial effect of Syperlymph preparation related to complex effect of cytokines and antimicrobial peptides directed to M. tuberculosis and macrophages, which forms the conditions for killing of mycobacteria.
Koval'chuk
L V
LV
Gvozdeva
Iu V
IuV
Gankovskaia
L V
LV
Chernousova
L N
LN
Smirnova
T G
TG
Larionova
E E
EE
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D018920:Coculture Techniques; D016207:Cytokines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
D023181:Antimicrobial Cationic Peptides; D016207:Cytokines