Influence of the natural ripening stage, cold storage, and ethylene treatment on the protein and IgE-binding profiles of green and gold kiwi fruit extracts.
Kiwi fruit is an important source of food allergens, the number and relevance of which are still the object of investigation. Following a comparative analysis of the protein profiles in SDS-PAGE and IgE immunoblotting, a significant influence of conditions such as the ripening stage and the extraction method on the composition of green and gold kiwi fruit extracts was observed. Furthermore, the experimental data indicate that, mostly in the green species, a ripe fruit may have a different concentration of total proteins and a different amount of single components when ripeness is reached by different means of postharvest handling, such as ethylene exposure with or without previous cold storage. In summary, this study emphasizes the level of complexity associated with the preparation of extracts when a known and defined concentration of proteins/allergens is requested.
Ciardiello
M Antonietta
MA
Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Napoli, Italy. ma.ciardiello@ibp.cnr.it
Giangrieco
Ivana
I
Tuppo
Lisa
L
Tamburrini
Maurizio
M
Buccheri
Marina
M
Palazzo
Paola
P
Bernardi
Maria Livia
ML
Ferrara
Rosetta
R
Mari
Adriano
A
D029042:Actinidia / Q000737:chemistry*; D052179:Antigens, Plant / Q000032:analysis / Q000276:immunology; D003080:Cold Temperature; D005030:Ethylenes / Q000494:pharmacology*; D005519:Food Preservation / Q000379:methods; D005638:Fruit / Q000737:chemistry / Q000254:growth & development* / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology
D052179:Antigens, Plant; D005030:Ethylenes; D010936:Plant Extracts; D010940:Plant Proteins; D007073:Immunoglobulin E; C036216:ethylene