[Study on fingerprints correlated with pharmacodynamic of Paeonia lacliflora and Glycyrrhiza uralensis effective compounds].
OBJECTIVE
To study the spasmolysisy activity constituents of the Radix Paeoniae alba and Radix et Rhizoma compound.


METHODS
The three effective compounds of Radix Paeoniae and Radix et Rhizoma were arrayed to eight groups by orthogonal design rat, serum samples were collected after administered in these groups, and these serum samples were used to carry out the experiments of serum pharmacology of spasmolysis. The inhibition ratios were assigned as pharmacodynamic variable X, the areas of characteristic peaks on HPLC fingerprints were assigned as variable Y, and the correlation of X and Y was studied in order to research the activity spasmolysisy compounds of effective constituents. Serum samples were analyzed by HPLC-ESI-MS/MS.


RESULTS
The correlation of fingerprints and pharmacology showed that four compounds were the main spasmolysisy activity compounds. The correlation parameter was greater than >0.4, whose value were 0.774, 0.678, 0.566 and 0.472 respectively, and their retention times were 8.03, 51.7, 54.59 and 72.43 min respectively. They were metabolites of effective compounds.


CONCLUSIONS
This study makes a valid approach for understanding the material foundation of TCM by establishing the correlation of fingerprints and pharmacology of activity compounds in vivo.
Shen
Lan
L
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. alansusu@sina.com
Zhang
Liang
L
Feng
Yi
Y
Xu
De-sheng
DS
Lin
Xiao
X
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology*; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D029596:Paeonia / Q000737:chemistry*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D004365:Drugs, Chinese Herbal