Anti-intercellular adhesion molecule-1 antibody's effect on noise damage.
OBJECTIVE
The purpose of this study was to investigate possible preventive effects of anti-intercellular adhesion molecule-1 antibody (anti-ICAM-1 Ab) on noise-induced cochlear damage as assessed by changes in auditory thresholds and cochlear blood flow.


METHODS
A controlled animal study. Pretreated rats with anti-ICAM-1 Ab or saline control, followed with exposure to 72 continuous hours of broad band noise (107 dB SPL), and 24 hours after noise exposure treated again with anti-ICAM-1 Ab or saline.


METHODS
Eighteen healthy male Fischer rats (200-250 g) were used. Sixteen were randomly selected to study noise-induced temporary threshold shifts. The remaining two rats were used to study cochlear blood flow (CBF), using laser Doppler flowmetry and blood pressure measurements.


RESULTS
Rats treated with anti-ICAM-1 Ab (1.875 mg/kg, intravenously) showed attenuated temporary threshold shifts (TTS) compared to controls. Both groups showed a partial threshold recovery 72 hours following noise exposure, normal for this noise exposure paradigm. Comparisons of baseline and post-treatment measurements of CBF and mean arterial blood pressure revealed no significant changes. Anti-ICAM-1 Ab animals displayed significantly lower mean auditory threshold shifts at all five test frequencies (P < .05) when compared to control.


CONCLUSIONS
Blocking the cascade of reactive oxygen species (ROS) generation by using anti-ICAM-Ab protects against noise-induced hearing loss.
Seidman
Michael D
MD
Department of Otolaryngology, Henry Ford Health System, Detroit, Michigan 48323, USA. mseidma1@hfhs.org
Tang
Wenxue
W
Shirwany
Najeeb
N
Bai
Uma
U
Rubin
Cory J
CJ
Henig
Joseph P
JP
Quirk
Wayne S
WS
D000818:Animals; D017306:Annexin A2 / Q000627:therapeutic use*; D001309:Auditory Threshold / Q000187:drug effects; D003051:Cochlea / Q000098:blood supply*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D006317:Hearing Loss, Noise-Induced / Q000188:drug therapy*; D007275:Injections, Intravenous; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008297:Male; D009622:Noise; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D012039:Regional Blood Flow / Q000187:drug effects; D009418:S100 Proteins / Q000627:therapeutic use*; D000099248:S100 Calcium Binding Protein A10
D017306:Annexin A2; D018799:Intercellular Adhesion Molecule-1; D017382:Reactive Oxygen Species; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10