title: Incidence of transfusion reactions and retention of procoagulant and anticoagulant factor activities in equine plasma.
abstract: BACKGROUND
  The extent of preservation of clotting factors and incidence of transfusion reactions to noncommercial equine plasma is not documented.
  
  
  OBJECTIVE
  Equine frozen plasma would retain its coagulation factor activity within the reference range and the incidence of transfusion reactions would be low.
  
  
  METHODS
  Ten plasma donor horses. Fifty clinically ill hospitalized horses receiving plasma were reviewed to determine the incidence of reactions.
  
  
  METHODS
  In vitro study and retrospective case review. Plasma was prepared by gravity sedimentation from whole blood refrigerated for 48 hours. The activities of factors VII through XII, antithrombin (AT), and Protein C were measured. Factor activities were compared for plasma samples obtained before blood collection (S0), after 48 hours of gravity sedimentation at 5 degrees C and after plasma separation (S1), and after 90 days of storage at -20 degrees C (S90). The medical records of 50 consecutive clinically ill horses receiving frozen plasma were reviewed to determine the incidence of transfusion reactions.
  
  
  RESULTS
  The combined effect of plasma harvest, gravity sedimentation, decantation, and freezing caused significant reductions in factors IX, (43%P= .0013), X, (33%P= .0001), XI, (48%P= .0008), AT, (10%P= .02), and Protein C (26%P= .0001). Activities for all factors analyzed, except factor X, remained within the reference ranges. Transfusion reactions were recorded for 5/50 horses.
  
  
  CONCLUSIONS
  Clotting factors, AT, and Protein C were well preserved. The incidence of reactions to frozen plasma was 10%.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. wilso716@cvm.msu.edu
authors.forename: E M
authors.initials: EM
authors.lastname: Wilson
authors.forename: S J
authors.initials: SJ
authors.lastname: Holcombe
authors.forename: A
authors.initials: A
authors.lastname: Lamar
authors.forename: J G
authors.initials: JG
authors.lastname: Hauptman
authors.forename: M B
authors.initials: MB
authors.lastname: Brooks
mesh_terms: D000818:Animals; D000991:Antithrombins / Q000378:metabolism*; D001779:Blood Coagulation Factors / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods / Q000662:veterinary*; D001803:Blood Transfusion / Q000662:veterinary*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D006736:Horses / Q000097:blood*; D011486:Protein C / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: D000991:Antithrombins; D001779:Blood Coagulation Factors; D011486:Protein C