title: Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays.
abstract: To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aoba, Aramaki, Sendai 980-8579, Japan.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murata
authors.forename: Michiaki
authors.initials: M
authors.lastname: Takeda
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000627:Aminophenols / Q000737:chemistry; D002460:Cell Line; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D046228:Microarray Analysis; D008839:Microelectrodes; D010084:Oxidation-Reduction; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D000627:Aminophenols; D011137:Polystyrenes; D000469:Alkaline Phosphatase; C026729:4-aminophenol