In-vitro effects of brain-derived neurotrophic factor on neural progenitor/stem cells from rat hippocampus.
Mounting evidences from in-vivo studies showed that brain-derived neurotrophic factor (BDNF) plays an important role in the neurogenesis, but the effects of BDNF are controversial because of neurogenesis affected by many factors in vivo. In this study, we investigated the effects of BDNF on the survival, proliferation, and differentiation of the neural progenitor/stem cells (NPCs) in vitro. The results showed that 40 ng/ml of BDNF significantly increased the number and diameter of neurospheres formed by NPCs; meanwhile, the TUNEL rates and lactate dehydrogenase release of NPCs were also inhibited. Tuj-1+ immunostaining showed that BDNF obviously induced the NPCs to differentiate into neurons and elongated the neurite. Our results implied that BDNF promotes the proliferation of NPCs and induces them to differentiate into neurons, and enhancement of the survival of NPCs probably is one of the mechanisms.
Li
Tingsong
T
Department of Neurology, Children's Hospital of Chongqing Medical University, Chongqing, PR China.
Jiang
Li
L
Zhang
Xiaoping
X
Chen
Hengsheng
H
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006624:Hippocampus / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D014404:Tubulin; C462941:beta3 tubulin, mouse; D007770:L-Lactate Dehydrogenase