In situ extraction of polar product of whole cell microbial transformation with polyethylene glycol-induced cloud point system.
A novel polyethylene glycol-induced cloud point system (PEG-CPS) was developed for in situ extraction of moderate polar product by setting a microbial transformation of benzaldehyde into L-phenylacetylcarbinol (L-PAC) with Saccharomyces cerevisiae (baker's yeast) as a model reaction. The biocompatibility of the microorganism in PEG-CPS was comparatively studied with a series of water-organic solvent two-phase partitioning systems. The tolerance of microorganism to the toxic substrate benzaldehyde was increased and the moderate polar product L-PAC was extracted into the surfactant-rich phase in the PEG-CPS. The novel PEG-CPS fills the gap of in situ extraction of polar product in microbial transformation left by water-organic solvent two-phase partitioning system. At the same time, the application of PEG-CPS in a microbial transformation also avoids expensive solvent when compared with that of aqueous two-phase system or CPS.
Wang
Zhilong
Z
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, PR China. zlwang@sjtu.edu.cn
Xu
Jian-He
JH
Zhang
Wenzhi
W
Zhuang
Baohua
B
Qi
Hanshi
H
D000096:Acetone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D002470:Cell Survival; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
D001547:Benzaldehydes; D000096:Acetone; D011092:Polyethylene Glycols; C035293:1-hydroxy-1-phenyl-2-propanone; D005947:Glucose