Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells.
Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.
Hirose
Etsuko
E
Department of Medical Technology, Nagoya University School of Health Science, 1-1-20 Daikou-minami, Higashi-ku, Nagoya, 461-8673, Japan.
Matsushima
Miyoko
M
Takagi
Kenzo
K
Ota
Yui
Y
Ishigami
Keiko
K
Hirayama
Tatsuya
T
Hayashi
Yuta
Y
Nakamura
Toshinobu
T
Hashimoto
Naozumi
N
Imaizumi
Kazuyoshi
K
Baba
Kenji
K
Hasegawa
Yoshinori
Y
Kawabe
Tsutomu
T
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D047309:Flavones; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D044949:Kaempferols / Q000276:immunology / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology; D051381:Rats; D015854:Up-Regulation / Q000235:genetics
D018926:Anti-Allergic Agents; D047309:Flavones; D044949:Kaempferols; C006552:kaempferol; D051547:Heme Oxygenase-1