title: Structure of the lipopolysaccharide core of Vibrio vulnificus type strain 27562.
abstract: The structure of the lipopolysaccharide core of Vibrio vulnificus type strain 27562 is presented. LPS hydrolysis gave two oligosaccharides, OS-1 and OS-2, as well as lipid A. NMR spectroscopic data corresponded to the presence of one Kdo residue, one beta-glucopyranose, three heptoses, one glyceric acid, one acetate, three PEtN, and one 5,7-diacylamido-3,5,7,9-tetradeoxynonulosonic acid residue (pseudaminic acid, Pse) in OS1. OS2 differed form OS 1 by the absence of glyceric acid, acetate, and Pse residues. Lipid A was analyzed for fatty acid composition and the following fatty acids were found: C14:0, C12:0-3OH, C16:0, C16:1, C14:0-3OH, C18:0, C18:1 in a ratio of 1:3:3:1:2.5:0.6:0.8.
authors.affiliation: Institute for Biological Sciences, National Research Council, Ont., Canada.
authors.forename: Evguenii
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Caroline
authors.initials: C
authors.lastname: Wilde
authors.forename: Erin M
authors.initials: EM
authors.lastname: Anderson
authors.forename: Alina
authors.initials: A
authors.lastname: Nakhamchik
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Lam
authors.forename: Dean A
authors.initials: DA
authors.lastname: Rowe-Magnus
mesh_terms: D000107:Acetylation; D002240:Carbohydrate Sequence; D005227:Fatty Acids / Q000737:chemistry; D015151:Immunoblotting; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D041261:Vibrio vulnificus / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D009844:Oligosaccharides