Identification of genes upregulated by pinewood nematode inoculation in Japanese red pine.
Pine wilt disease caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle, has destroyed huge areas of pine forest in East Asia, including Japan, China and Korea. No protection against PWN has been developed, and the responses of pine trees at the molecular level are unrecorded. We isolated and analyzed upregulated or newly induced genes from PWN-inoculated Japanese red pine (Pinus densiflora Sieb. et Zucc.) by using an annealing control primer system and suppression subtractive hybridization. Significant changes occurred in the transcript abundance of genes with functions related to defense, secondary metabolism and transcription, as the disease progressed. Other gene transcripts encoding pathogenesis-related proteins, pinosylvin synthases and metallothioneins were also more abundant in PWN-inoculated trees than in non-inoculated trees. Our report provides fundamental information on the molecular mechanisms controlling the biochemical and physiological responses of Japanese red pine trees to PWN invasion.
Shin
Hanna
H
Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Korea. hslee@forest.go.kr
Lee
Hyoshin
H
Woo
Kwan-Soo
KS
Noh
Eun-Woon
EW
Koo
Yeong-Bon
YB
Lee
Kyung-Joon
KJ
D000818:Animals; D017931:DNA Primers; D020869:Gene Expression Profiling; D015723:Gene Library; D017343:Genes, Plant; D006790:Host-Parasite Interactions; D009348:Nematoda / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D028223:Pinus / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*; D010935:Plant Diseases; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D017931:DNA Primers