Sandwich-type electrochemiluminescence immunosensor based on N-(aminobutyl)-N-ethylisoluminol labeling and gold nanoparticle amplification.
A novel electrochemiluminescence (ECL) sandwich-type immunosensor for human immunoglobulin G (hIgG) on a gold nanoparticle modified electrode was developed by using N-(aminobutyl)-N-ethylisoluminol (ABEI) labeling. The primary antibody, goat-anti-human IgG was first immobilized on a gold nanoparticle modified electrode, then the antigen (human IgG) and the ABEI-labeled second antibody was conjugated successively to form a sandwich-type immunocomplex. ECL was carried out with a double-step potential in carbonate buffer solution (CBS) containing 1.5mM H(2)O(2). The ECL intensity increased linearly with the concentration of hIgG over the range 5.0-100 ng/mL. The limit of detection was 1.68 ng/mL (S/N=3). The relative standard deviation was 3.79% at 60 ng/mL (n=9). The present immunosensor is simple and sensitive. It has been successfully applied to the detection of hIgG in human serums.
Tian
Dayong
D
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Duan
Chunfeng
C
Wang
Wei
W
Li
Na
N
Zhang
Hao
H
Cui
Hua
H
Lu
Yingyu
Y
D000818:Animals; D000906:Antibodies; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006041:Goats; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D008163:Luminescent Measurements; D008165:Luminol; D053768:Metal Nanoparticles
D000906:Antibodies; D007074:Immunoglobulin G; D008165:Luminol; D006046:Gold