Authentication of Panax ginseng from its adulterants by PCR-RFLP and ARMS.
As a widely used and expensive herbal medicine, Panax ginseng has many adulterants in the commercial market. PCR-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) based on 5S rDNA sequence analysis were applied to identify two common adulterants of P. ginseng. The sizes of 5S rRNA gene non-transcribed spacers (NTS) sequences in P. ginseng and its adulterants were determined, ranging from 143 to 424 bp. The PCR product of P. ginseng only could be digested among the tested specimens because of its specific SpeI restriction site found in the 5S rDNA sequence. In addition, P. ginseng was successfully identified from compound medicinal preparations and from the Single-Taste medicines. These results suggest that the methods are able to authenticate P. ginseng.
Diao
Ying
Y
Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan, P. R. China.
Lin
Xian-Ming
XM
Liao
Chao-Lin
CL
Tang
Chun-Zi
CZ
Chen
Zhong-Jian
ZJ
Hu
Zhong-Li
ZL
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D001483:Base Sequence; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D004340:Drug Contamination / Q000517:prevention & control*; D029001:Herbal Medicine / Q000592:standards*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D005894:Panax / Q000737:chemistry / Q000235:genetics*; D010936:Plant Extracts / Q000235:genetics*; D018515:Plant Leaves; D011786:Quality Control; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D010936:Plant Extracts