Rapid and sensitive assay for trantinterol, a novel beta(2)-adrenoceptor agonist, in human plasma using liquid chromatography-tandem mass spectrometry.
A rapid and sensitive assay for trantinterol, a novel beta(2)-adrenoceptor agonist, in human plasma has been developed. Samples containing the analyte and internal standard, clenbuterol, were analyzed by liquid chromatography-tandem mass spectrometry after liquid-liquid extraction with diethyl ether:dichloromethane (60:40, v/v). Separation was performed on a Venusil MP C(18) column (50 mm x 4.6 mm, 5 microm) using methanol:1% formic acid (50:50, v/v) as mobile phase and monitored by multiple reaction monitoring of the precursor-to-product ion transitions of trantinterol at m/z 311.2-->238.1 and clenbuterol at m/z 277.2 --> 203.1. The total run time was only 1.5 min and the method was linear over the concentration range 1-1000 pg/mL with a lower limit of quantitation of 1 pg/mL. Intra- and inter-day precisions (relative standard deviation) were below 7% and 12%, respectively, with accuracy (relative error) below 8%. The method was successfully applied to a pharmacokinetic study involving oral administration of a 50 microg trantinterol tablet to healthy volunteers.
Sun
Yantong
Y
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun, China.
Liu
Mingyuan
M
Zhang
Jiwen
J
Jiang
Yao
Y
Yu
Weisong
W
Fawcett
John Paul
JP
Gu
Jingkai
J
D058647:Adrenergic alpha-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000097:blood* / Q000493:pharmacokinetics; D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002976:Clenbuterol / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008297:Male; D011786:Quality Control; D053719:Tandem Mass Spectrometry
D058647:Adrenergic alpha-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; C542064:trantinterol; D002976:Clenbuterol