title: Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags.
abstract: BACKGROUND
  Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state. Genes induced by desiccation stress have been successfully enumerated in nematodes; however we have little knowledge of gene regulation by Antarctic nematodes which can survive multiple environmental stresses. To address this problem we investigated the genetic responses of a nematode species, Plectus murrayi, that is capable of tolerating Antarctic environmental extremes, in particular desiccation and freezing. In this study, we provide the first insight into the desiccation induced transcriptome of an Antarctic nematode through cDNA library construction and suppressive subtractive hybridization.
  
  
  RESULTS
  We obtained 2,486 expressed sequence tags (ESTs) from 2,586 clones derived from the cDNA library of desiccated P. murrayi. The 2,486 ESTs formed 1,387 putative unique transcripts of which 523 (38%) had matches in the model-nematode Caenorhabditis elegans, 107 (7%) in nematodes other than C. elegans, 153 (11%) in non-nematode organisms and 605 (44%) had no significant match to any sequences in the current databases. The 1,387 unique transcripts were functionally classified by using Gene Ontology (GO) hierarchy and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The results indicate that the transcriptome contains a group of transcripts from diverse functional areas. The subtractive library of desiccated nematodes showed 80 transcripts differentially expressed during desiccation stress, of which 28% were metabolism related, 19% were involved in environmental information processing, 28% involved in genetic information processing and 21% were novel transcripts. Expression profiling of 14 selected genes by quantitative Real-time PCR showed 9 genes significantly up-regulated, 3 down-regulated and 2 continuously expressed in response to desiccation.
  
  
  CONCLUSIONS
  The establishment of a desiccation EST collection for Plectus murrayi, a useful model in assessing the structural, physiological, biochemical and genetic aspects of multiple stress tolerance, is an important step in understanding the genome level response of this nematode to desiccation stress. The type of transcript analysis performed in this study sets the foundation for more detailed functional and genome level analyses of the genes involved in desiccation tolerance in nematodes.
authors.affiliation: Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA. adhikaribn@hotmail.com
authors.forename: Bishwo N
authors.initials: BN
authors.lastname: Adhikari
authors.forename: Diana H
authors.initials: DH
authors.lastname: Wall
authors.forename: Byron J
authors.initials: BJ
authors.lastname: Adams
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D016000:Cluster Analysis; D019295:Computational Biology; D003681:Dehydration / Q000235:genetics*; D020224:Expressed Sequence Tags; D005615:Freezing; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D017238:Genes, Helminth; D049751:Genome, Helminth; D009348:Nematoda / Q000235:genetics* / Q000502:physiology*; D018510:RNA, Helminth / Q000235:genetics; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D018510:RNA, Helminth