title: Evidence of parietal amine oxidase activity in Solanum torvum Sw. stem calli after Ralstonia solanacearum inoculation.
abstract: Calli induced from Solanum torvum stem explants were inoculated with Ralstonia solanacearum under partial vacuum. All calli showed a hypersensitive response after infiltration. Furthermore, amine oxidase activity with aldehyde and H(2)O(2) production was detected in semi-purified cell walls of calli infiltrated by the bacteria. Due to its preferential affinity for monoamines, this enzyme is supposed to have monoamine oxidase-like (MAO-like) activity. Moreover, the presence of hydroxyl radicals in the aromatic cycle alters the oxidative deamination kinetics of potential substrates. Indeed, the oxidation of dopamine (+2, OH) was shown to be faster than that of tyramine (+1, OH), which in turn was faster than that of phenylethylamine (0, OH). The MAO-like catalytic activity was significantly inhibited by some reducing agents such as sodium bisulphite and cysteine, and also by tryptamine under anaerobiosis. This latter result suggested that the prosthetic group of the MAO-like enzyme could be a tyrosine-derived 6-hydroxytopaquinone structure. Finally, the sigmoid kinetics of the MAO-like enzyme in semi-purified cell walls did not correspond to that expected for a purified MAO, suggesting that the kinetics were affected by some factors present in cell walls.
authors.affiliation: Université de La Réunion, Faculté des Sciences et Technologies, UMR "Peuplements végétaux et bioagresseurs en milieu tropical" Université de La Réunion-CIRAD, 15 avenue René Cassin, BP 7151, 97715 Saint Denis messag cedex 9, La Réunion, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Aribaud
authors.forename: Michel
authors.initials: M
authors.lastname: Noirot
authors.forename: Anne
authors.initials: A
authors.lastname: Gauvin
authors.forename: Christine
authors.initials: C
authors.lastname: Da Silva-Robert
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fock
authors.forename: Hippolyte
authors.initials: H
authors.lastname: Kodja
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology*; D004298:Dopamine / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D008995:Monoamine Oxidase / Q000378:metabolism*; D043368:Ralstonia solanacearum / Q000502:physiology*; D032322:Solanum / Q000201:enzymology* / Q000382:microbiology*; D014439:Tyramine / Q000378:metabolism
chemical_list: D000447:Aldehydes; D006861:Hydrogen Peroxide; D008995:Monoamine Oxidase; D004298:Dopamine; D014439:Tyramine