Production of cryoprotectant extracellular polysaccharide substances (EPS) by the marine psychrophilic bacterium Colwellia psychrerythraea strain 34H under extreme conditions.
Extracellular polysaccharide substances (EPS) play critical roles in microbial ecology, including the colonization of extreme environments in the ocean, from sea ice to the deep sea. After first developing a sugar-free growth medium, we examined the relative effects of temperature, pressure, and salinity on EPS production (on a per cell basis) by the obligately marine and psychrophilic gamma-proteobacterium, Colwellia psychrerythraea strain 34H. Over growth-permissive temperatures of approximately 10 to -4 degrees C, EPS production did not change, but from -8 to -14 degrees C when samples froze, EPS production rose dramatically. Similarly, at growth-permissive hydrostatic pressures of 1-200 atm (1 atm = 101.325 kPa) (at -1 and 8 degrees C), EPS production was unchanged, but at higher pressures of 400 and 600 atm EPS production rose markedly. In salinity tests at 10-100 parts per million (and -1 and 5 degrees C), EPS production increased at the freshest salinity tested. Extreme environmental conditions thus appear to stimulate EPS production by this strain. Furthermore, strain 34H recovered best from deep-freezing to -80 degrees C (not found for Earthly environments) if first supplemented with a preparation of its own EPS, rather than other cryoprotectants like glycerol, suggesting EPS production as both a survival strategy and source of compounds with potentially novel properties for biotechnological and other applications.
Marx
Joseph G
JG
School of Oceanography, University of Washington, Seattle, WA98195, USA.
Carpenter
Shelly D
SD
Deming
Jody W
JW
D003080:Cold Temperature; D003451:Cryoprotective Agents / Q000378:metabolism*; D003470:Culture Media; D005615:Freezing; D020563:Gammaproteobacteria / Q000378:metabolism*; D006874:Hydrostatic Pressure; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D054712:Salinity; D014871:Water Microbiology
D003451:Cryoprotective Agents; D003470:Culture Media; D011135:Polysaccharides, Bacterial