Fourier transform infrared immunosensors for model hapten molecules.
We report experimental results concerning the detection of 2,4-dinitrophenol, under its free form or coupled to human serum albumin using Fourier transform infrared spectroscopy-based sensors. Competitive immunoreactions were carried out using several anti-dinitrophenol monoclonal antibodies. Comparison with enzyme-linked immunosorbent assays in competition is given for standard operating conditions. FTIR detection limits are comparable to those obtained by ELISA. The limits of detection are about 5-15 ng/mL for the coupled DNP. Using the LO-DNP61 antibody, a detection limit of congruent with 5 ng/mL was also estimated for the free DNP molecules but is much higher for the other antibodies.
Gosselin
E
E
Centre de Recherche en Modélisation Moléculaire, Université de Mons-Hainaut, Place du Parc, 20, B-7000 Mons, Belgium.
Gorez
M
M
Voué
M
M
Denis
O
O
Conti
J
J
Popovic
N
N
Van Cauwenberge
A
A
Noel
E
E
De Coninck
J
J
D000911:Antibodies, Monoclonal / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006241:Haptens / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D000911:Antibodies, Monoclonal; D006241:Haptens