title: Incorporation of tellurocysteine into glutathione transferase generates high glutathione peroxidase efficiency.
abstract: A rival to native peroxidase! An existing binding site for glutathione was combined with the catalytic residue tellurocysteine by using an auxotrophic expression system to create an engineered enzyme that functions as a glutathione peroxidase from the scaffold of a glutathione transferase (see picture). The catalytic activity of the telluroenzyme in the reduction of hydroperoxides by glutathione is comparable to that of native glutathione peroxidase.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Road, Changchun 130012, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Liu
authors.forename: Louis A
authors.initials: LA
authors.lastname: Silks
authors.forename: Cuiping
authors.initials: C
authors.lastname: Liu
authors.forename: Morgane
authors.initials: M
authors.lastname: Ollivault-Shiflett
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Guimin
authors.initials: G
authors.lastname: Luo
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Hou
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D002384:Catalysis; D003545:Cysteine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: C540155:tellurocysteine; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D003545:Cysteine