The effects of resveratrol and tannic acid on apoptosis in colon adenocarcinoma cell line.
OBJECTIVE
To investigate the effects of resveratrol and tannic acid on apoptosis, and Bcl-2 homologous antagonist/killer (Bak) and fas associated death domain (FADD) proteins in the CaCo-2 cell line.


METHODS
In the present study, resveratrol and tannic acid were administrated in the CaCo-2 cell line at doses of 25, 50, and 100 microM. The CaCo-2 cells were grown and cultured in the Medical Biology Department, Eskisehir Osmangazi University, Eskisehir, Turkey in 2007. The effects of these agents on apoptotic index were determined by Apop Taq peroxidase kit and their effects on the ratios of Bak and FADD proteins by the immunohistochemical staining method at 24, 48, and 72 hours. Stained and non-stained cells in 30 separate areas of the 3 separate chamber slides, prepared for each group, were counted. The percentage of apoptosis, and Bak and FADD proteins was calculated with the control. Mean +/- standard error values were calculated for the 3 experiments.


RESULTS
Apoptotic index, Bak protein percentage ratio, and FADD protein percentage ratio values in all groups that received tannic acid and resveratrol increased when compared within the groups. This increase was found to be time and dose independent in all parameters.


CONCLUSIONS
Cells undergo apoptosis in 2 pathways (mitochondrial and death receptor) in resveratrol and tannic acid induced CaCo-2 cells.
Cosan
Didem
D
Department of Medical Biology, Eskisehir Osmangazi University, Medical Faculty, Eskisehir, Turkey. dcosan@ogu.edu.tr
Soyocak
Ahu
A
Basaran
Ayse
A
Degirmenci
Irfan
I
Gunes
Hasan V
HV
D000230:Adenocarcinoma / Q000473:pathology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D053200:Fas-Associated Death Domain Protein / Q000032:analysis; D006801:Humans; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013634:Tannins / Q000494:pharmacology*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000032:analysis
D000972:Antineoplastic Agents, Phytogenic; C505533:FADD protein, human; D053200:Fas-Associated Death Domain Protein; D013267:Stilbenes; D013634:Tannins; D050998:bcl-2 Homologous Antagonist-Killer Protein; D000077185:Resveratrol