title: P-glycoprotein-mediated transport of moxifloxacin in a Calu-3 lung epithelial cell model.
abstract: Moxifloxacin (MXF) is a fluoroquinolone antibiotic that is effective against respiratory infections. However, the mechanisms of MXF lung diffusion are unknown. Active transport in other tissues has been suggested for several members of the fluoroquinolone family. In this study, transport of MXF was systematically investigated across a Calu-3 lung epithelial cell model. MXF showed polarized transport, with the secretory permeability being twice as high as the absorptive permeability. The secretory permeability was concentration dependent (apparent P(max) = 13.6 x 10(-6) cm x s(-1); apparent K(m) = 147 microM), suggesting saturated transport at concentrations higher than 350 microg/ml. The P-glycoprotein inhibitor PSC-833 inhibited MXF transport in both directions, whereas probenecid, a multidrug resistance-related protein inhibitor, appeared to have no effect in the Calu-3 model. Moreover, rifampin, a known inducer of efflux transport proteins, upregulated the expression of P-glycoprotein in Calu-3 cells and enhanced MXF active transport. In conclusion, this study clearly indicates that MXF is subject to P-glycoprotein-mediated active transport in the Calu-3 model. This P-glycoprotein-dependent secretion may lead to higher MXF epithelial lining fluid concentrations than those in plasma. Furthermore, drug-drug interactions may be expected when MXF is combined with other P-glycoprotein substrates or modulators.
authors.affiliation: INSERM, ERI-23, Pôle Biologie Santé, Poitiers, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Brillault
authors.forename: Whocely Victor
authors.initials: WV
authors.lastname: De Castro
authors.forename: Thomas
authors.initials: T
authors.lastname: Harnois
authors.forename: Alain
authors.initials: A
authors.lastname: Kitzis
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Olivier
authors.forename: William
authors.initials: W
authors.lastname: Couet
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000502:physiology*; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D001372:Aza Compounds / Q000493:pharmacokinetics*; D001692:Biological Transport; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000378:metabolism; D024841:Fluoroquinolones; D006801:Humans; D008168:Lung / Q000378:metabolism*; D000077266:Moxifloxacin; D011804:Quinolines / Q000493:pharmacokinetics*; D012293:Rifampin / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000890:Anti-Infective Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin; D012293:Rifampin