title: Preparation and in vitro evaluation of mucoadhesion and permeation enhancement of thiolated chitosan-pHEMA core-shell nanoparticles.
abstract: The aim of the present work was to evaluate the in vitro mucoadhesion and permeation enhancement properties of thiolated chitosan (chitosan-glutathione) coated poly(hydroxyl ethyl methacrylate) nanoparticles. Core-shell nanoparticles were prepared by radical emulsion polymerization method initiated by cerium(IV) ammonium nitrate. Different molecular weights of chitosan were utilized for nanoparticles preparation. The physicochemical properties of nanoparticles were characterized by size, zeta potential, and thiol content. Incorporation of fluorescein isothiocyanate dextran (FD4, MW 4400 Da), which was used as the model macromolecule, was achieved by incubation method. The intestinal mucoadhesion and penetration enhancement properties of nanoparticles were investigated using excised rat jejunum. All nanoparticle systems showed mucoadhesion and improved apparent permeation coefficient (P(app)) of FD4. Nanoparticles prepared by thiolated chitosan with medium molecular weight revealed the most mucoadhesion and penetration enhancement properties.
authors.affiliation: Novel Drug Delivery Systems Laboratory, Faculty of Pharmacy, Medical Sciences/University of Tehran, Tehran, Iran. famoghaddam@razi.tums.ac.ir
authors.forename: Firooze Aghaei
authors.initials: FA
authors.lastname: Moghaddam
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atyabi
authors.forename: Rassoul
authors.initials: R
authors.lastname: Dinarvand
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D048271:Chitosan / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats
chemical_list: D003911:Dextrans; D004337:Drug Carriers; D008689:Methacrylates; C015219:fluorescein isothiocyanate dextran; C005044:hydroxyethyl methacrylate; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate