[Effects of mechanical stress on the formation and expression of Cbfalpha1 in vitro].
OBJECTIVE
To evaluate the effects of mechanical stress on the formation and expression of core binding factor alpha1 (Cbfalpha1) in MG-63 cells cultured on titanium in vitro.


METHODS
MG-63 cells cultured on the titanium were subjected to a centrifugal force (2.205 N) 15 min per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfalpha1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force (P<0.05). Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfalpha1 and the relative mRNA level of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significant (P<0.05).


CONCLUSIONS
Mechanical stress are beneficial to the formation and expression of Cbfalpha1.
Wang
Xue-jin
XJ
Dept. of Stomatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
Hao
Xiao-ning
XN
Liu
Wei
W
Peng
Wei
W
Xu
Ying-hua
YH
D050659:Core Binding Factor Alpha 1 Subunit; D066298:In Vitro Techniques; D012333:RNA, Messenger; D013314:Stress, Mechanical
D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger