Marked induction of inducible nitric oxide synthase and tumor necrosis factor-alpha in rat CD40+ microglia by comparison to CD40- microglia.
There may be two subtypes of microglia (MG) at least in the CNS. We separated the two types from rat mixed glial culture. mRNAs and proteins for inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNFalpha) were more induced in CD40(+) MG than CD40(-) MG after LPS stimulation. Although the expression level of LPS receptors showed a little difference between the subtypes, LPS-induced degradation of phosphorylated IkappaBalpha was marked in CD40(+) MG. These results strongly suggest that CD40(+) MG produce larger amount of NO and TNFalpha to exhibit neurotoxic action under certain pathological conditions in brains.
Kawahara
Kohichi
K
Department of Molecular Cell Function, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan.
Yoshida
Akira
A
Koga
Kiyohiro
K
Yokoo
Sumika
S
Kuniyasu
Akihiko
A
Gotoh
Tomomi
T
Sawada
Makoto
M
Nakayama
Hitoshi
H
D000818:Animals; D019013:CD40 Antigens / Q000096:biosynthesis* / Q000172:deficiency / Q000378:metabolism; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000235:genetics / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology / Q000633:toxicity; D008070:Lipopolysaccharides / Q000502:physiology; D017628:Microglia / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000633:toxicity; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity
D019013:CD40 Antigens; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II