Small volume resuscitation in a rat model of heatstroke.
BACKGROUND
Herein, we compared the effectiveness of different small volume resuscitation in a rat model of heatstroke.


METHODS
Anesthetized rats, immediately after the onset of heatstroke, were randomly divided into 5 groups and given the following: (a) nothing; (b) 0.9% NaCl (1-10 mL/kg of body weight, i.v.); (c) hydroxyethyl starch (HAES) (6%, 1-10 mL/kg of body weight, i.v.); (d) 7.2% NaCl (1-10 mL/kg of body weight, i.v.); and (e) hyper-HAES (6% HAES plus 7.2% NaCl, 1-10 mL/kg of body weight, i.v.).


RESULTS
When the untreated or 0.9% NaCl (1-5 mL/kg of body weight)-treated rats underwent heat stress, their survival time values were found to be 20 to 22 minutes. Resuscitation with 10 mL/kg of body weight of 0.9% NaCl, 6% HAES, 7.2% NaCl, or hyper-HAES, their survival time values, respectively, are 93+/-6, 101+/-12, 154+/-18, or 286+/-21. Apparently, the order of effectiveness in resuscitation of heatstroke is hyper-HAES>7.2% NaCl>0.9% NaCl or 6% HAES. The heatstroke-induced hypotension, cerebral ischemia and hypoxia, hypercoagulable state, activated inflammation, and hepatic and renal dysfunction can be significantly reduced by hyper-HAES.


CONCLUSIONS
Our results suggest that hyper-HAES seems superior to 7.2% NaCl or HAES alone in resuscitation of heatstroke. The benefit of hyper-HAES during heatstroke is related to restoration of normal multiorgan function.
Liu
Chia-Chyuan
CC
Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
Cheng
Bor-Chih
BC
Lin
Mao-Tsun
MT
Lin
Hung-Jung
HJ
D000818:Animals; D002561:Cerebrovascular Disorders / Q000209:etiology / Q000628:therapy; D004195:Disease Models, Animal; D005440:Fluid Therapy / Q000379:methods; D018883:Heat Stroke / Q000150:complications / Q000628:therapy*; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage; D007674:Kidney Diseases / Q000209:etiology / Q000628:therapy; D008107:Liver Diseases / Q000209:etiology / Q000628:therapy; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology / Q000628:therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation / Q000379:methods*; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage; D012965:Sodium Chloride / Q000008:administration & dosage; D019851:Thrombophilia / Q000209:etiology / Q000628:therapy
D006895:Hydroxyethyl Starch Derivatives; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride