White matter changes in HIV-1 infected brains: a combined gross anatomical and ultrastructural morphometric investigation of the corpus callosum.
OBJECTIVE
The HIV-1 associated cognitive/motor complex is characterized by cognitive, motor and behavioral disturbances. Besides a significant loss of neurons in the cerebral cortex and subcortical nuclei, a possible morphological substrate of this complex is also given by changes of the white matter as seen in HIV-1 leucoencephalopathy (HIVL), which is characterized by widespread diffuse pallor of myelin and the presence of gliomesenchymal nodules with multinucleated giant cells.


METHODS
The corpus callosum as a sensitive marker for damage of the cerebral white matter was investigated by morphometry both at the macroscopic and electronmicroscopic level.


RESULTS
In HIV-1 infected brains, a significant decrease of the profile area of the whole corpus callosum as well as of its different parts was noted. The absolute number of nerve fibers was significantly decreased, in particular in the frontal and occipital parts of the corpus callosum. Moreover, several morphometric parameters for nerve fibers, axons and myelin sheaths indicate in some areas a reduction of nerve fibers and axons, as well as a diminished myelin sheath thickness, whereas, in other regions, swelling of axons and myelin sheaths was observed.


CONCLUSIONS
The observed changes are considered to represent subtle changes affecting nerve fibers before histological evidence of HIVL, and might represent one aspect of the morphological substrates preceeding the development of the HIV-1 related cognitive/motor complex.
Wohlschlaeger
J
J
Institute of Pathology and Neuropathology, University Hospital of Essen, University of Duisburg-Essen, Germany.
Wenger
E
E
Mehraein
P
P
Weis
S
S
D000328:Adult; D001253:Astrocytes / Q000473:pathology / Q000648:ultrastructure; D001369:Axons / Q000473:pathology / Q000648:ultrastructure; D001921:Brain / Q000473:pathology*; D002452:Cell Count; D003337:Corpus Callosum / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D015658:HIV Infections / Q000473:pathology*; D015497:HIV-1; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D017628:Microglia / Q000473:pathology / Q000648:ultrastructure; D008875:Middle Aged; D009186:Myelin Sheath / Q000473:pathology / Q000648:ultrastructure; D009412:Nerve Fibers / Q000473:pathology / Q000648:ultrastructure