Bioluminescence intensity difference observed in luminous bacteria groups with different motility.
OBJECTIVE
The aim of this study was to compare the luminescent intensity of bioluminescence from marine luminous bacteria with different motility.


RESULTS
Luminescent bacteria were separated according to their motility using a microfluidic device. The cell densities of the separated samples were measured using a counting plate. The luminescent intensity of the separated samples was measured using a luminometer. The luminescent intensity per cell was calculated, and the values from the mobile (swimmers) and the nonmobile cells (nonswimmers) per single cell were compared; as a result, the former were proved to be larger than the latter.


CONCLUSIONS
Microfluidics were shown to be effective for the separation of bioluminescent bacteria and the bioluminescent intensity difference per cell was recognized with this experiment.


CONCLUSIONS
This study introduced for the first time a method to examine the individual cell function of Photobacterium kishitanii.
Sasaki
S
S
School of Bionics, Tokyo University of Technology, Tokyo, Japan. sasaki@bs.teu.ac.jp
Okamoto
T
T
Fujii
T
T
D000818:Animals; D015169:Colony Count, Microbial; D003470:Culture Media; D049832:Decapodiformes / Q000382:microbiology; D008163:Luminescent Measurements; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D009068:Movement; D010776:Photobacterium / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D012623:Seawater / Q000737:chemistry
D003470:Culture Media