title: Polymorphism in ICAM-1, PECAM-1, E-selectin, and L-selectin genes in Tunisian patients with inflammatory bowel disease.
abstract: BACKGROUND
  Ulcerative colitis (UC) and Crohn's disease (CD) are chronic intestinal disorders characterized by immune dysregulation and leukocytes recruitment into gastrointestinal tract. Cell adhesion molecules (CAM) mediate the extravasation of leukocytes and their accumulation in inflamed intestinal mucosa. Recently, CAM genes have been implicated in determining susceptibility to UC and CD. We investigate seven mutations in CAM: G241R and K469E in ICAM-1, V125L in PECAM-1, G98T, S128R, and L554F in E-selectin and F206L in L-selectin in 197 Tunisian patients (73 with UC and 124 with CD) and 194 controls. These polymorphisms were detected by polymerase chain reaction sequence-specific primers and restriction enzyme analysis.
  
  
  RESULTS
  A significant increase in allele frequencies of 206L of L-selectin and the associated genotype F/L was observed in both patients with UC and CD compared with controls. Subgroup analysis showed that the L206 allele and F/L206 genotype frequencies were significantly increased in UC patients with left-sided type; whereas, the F/L206 genotype was significant in CD patients with ileocolonic location and stricturing behavior compared with controls. No significant differences in allele or genotype frequencies were observed for ICAM-1 K469E, E-selectin, and PECAM-1 polymorphisms between UC patients, CD patients, and controls.
  
  
  CONCLUSIONS
  We found an association of inflammatory bowel disease with allele L206 of L-selectin gene, whereas genotype L/F was associated with a subgroup of UC (left-sided type) and CD patients with more extensive location of disease and stricturing behavior. However, further studies are needed to confirm our findings.
authors.affiliation: Laboratory of Immunology, EPS Charles Nicolle, Tunis, Tunisia.
authors.forename: Dorra
authors.initials: D
authors.lastname: Khazen
authors.forename: Saloua
authors.initials: S
authors.lastname: Jendoubi-Ayed
authors.forename: Walid Ben
authors.initials: WB
authors.lastname: Aleya
authors.forename: Imen
authors.initials: I
authors.lastname: Sfar
authors.forename: Leila
authors.initials: L
authors.lastname: Mouelhi
authors.forename: Samira
authors.initials: S
authors.lastname: Matri
authors.forename: Tawfik
authors.initials: T
authors.lastname: Najjar
authors.forename: Azza
authors.initials: A
authors.lastname: Filali
authors.forename: Yousr
authors.initials: Y
authors.lastname: Gorgi
authors.forename: Taieb Ben
authors.initials: TB
authors.lastname: Abdallah
authors.forename: Khaled
authors.initials: K
authors.lastname: Ayed
mesh_terms: D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000235:genetics; D003424:Crohn Disease / Q000235:genetics; D019040:E-Selectin / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D019041:L-Selectin / Q000235:genetics; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin