Composition of the ANTIGENome of Helicobacter pylori defined by human serum antibodies.
Helicobacter pylori is the most prevalent human pathogen and although, it remains silent in most individuals for lifetime, colonization may develop into severe gastric and duodenal conditions. Rapidly developing resistance to antibiotic treatment urgently calls for the development of effective vaccines. We determined the ANTIGENome of two clinical isolates of H. pylori, KTH-Ca1 and KTH-Du, derived from patients with gastric cancer and duodenal ulcer, respectively. Using disease-relevant human sera from well-characterized donors we identified 124 annotated ORFs and 54 non-annotated peptides as antigens. Through in vitro validation assays we selected the 20 most promising vaccine candidates. Importantly, two candidates represent proteins that were previously shown to provide protection in models of H. pylori infection. One of the most frequently selected and conserved protein, the siderophore-dependent transporter HP1341, was confirmed to show high reactivity with human serum IgGs. These analyses provide the means to identify novel antigens for the selection of vaccine candidates, as well as disease associated biomarkers.
Meinke
Andreas
A
Intercell AG, Campus Vienna Biocenter 5, A-1030 Vienna, Austria.
Storm
Martin
M
Henics
Tamás
T
Gelbmann
Dieter
D
Prustomersky
Sonja
S
Kovács
Zoltán
Z
Minh
Duc Bui
DB
Noiges
Birgit
B
Stierschneider
Ulrike
U
Berger
Manfred
M
von Gabain
Alexander
A
Engstrand
Lars
L
Nagy
Eszter
E
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D004381:Duodenal Ulcer / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics; D016680:Genome, Bacterial; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000382:microbiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes