title: Identification of a Taq DNA polymerase inhibitor from the red seaweed Symphyocladia latiuscula.
abstract: Two inhibitors of Taq DNA polymerase were isolated from the marine red alga Symphyocladia latiuscula. The inhibitors were purified by methanol extraction, molecular fractionation below 3000 MW and reverse-phase HPLC. The purified compound SL-1 containing three bromines was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol (C7H5Br3O3: MW374) by NMR and MS analyses. The purified compound SL-2 was identified as 2,3, 6-tribromo-4,5-dihydroxybenzyl methyl ether(C8H7Br3O3: MW388). In a 25-microl reaction mixture containing 1.5 units of Taq DNA polymerase, the enzyme was completely inhibited by 0.5 microg SL-1 or 5 microg SL-2.
authors.affiliation: Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung 210-702, Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Jin
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Oh
authors.forename: Deuk Hee
authors.initials: DH
authors.lastname: Jin
authors.forename: Yong Ki
authors.initials: YK
authors.lastname: Hong
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004987:Ethers; D006842:Hydrocarbons, Brominated / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000432:Methanol / Q000737:chemistry; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D000461:Rhodophyta / Q000737:chemistry*; D013057:Spectrum Analysis; D019914:Taq Polymerase / Q000037:antagonists & inhibitors*
chemical_list: C439224:2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether; D004791:Enzyme Inhibitors; D004987:Ethers; D006842:Hydrocarbons, Brominated; D019914:Taq Polymerase; D000432:Methanol