Immunomodulation of acute experimental spinal cord injury with human immunoglobulin G.
Immunomodulation of acute spinal cord injury may inhibit the activity of specific inflammatory cascades and result in recovery of motor function. In this study, evaluation of the protective effect of a well-known anti-inflammatory immunomodulator, immunoglobulin G (IgG), was conducted in rats after a 50 g/cm contusion spinal cord injury. Following injury, 400 mg/kg of IgG was administered to the treatment group. Twenty-four hours later, animals were assessed functionally via an inclined plane and the Basso-Beattie-Bresnahan motor scale and compared to controls. Tissue was reviewed for myeloperoxidase activiy (MPO) and lipid peroxidation (LPO), and electron microscopy was conducted to assess tissue ultrastructure. Significant functional preservation was observed in the IgG treatment group. In addition, biochemical assays revealed decreased MPO activity, and electron microscopic views of tissue showed preserved ultrastructure. IgG treatment following acute contusion injury to the rat spinal cord confers functional and structural neuroprotection.
Gok
Beril
B
Ankara Ataturk Research and Education Hospital, Department of Neurological Surgery, Ankara, Turkey. berylgok@gmail.com
Sciubba
Daniel M
DM
Okutan
Ozerk
O
Beskonakli
Etem
E
Palaoglu
Selcuk
S
Erdamar
Husamettin
H
Sargon
Mustafa F
MF
D000818:Animals; D001522:Behavior, Animal; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000627:therapeutic use*; D007155:Immunologic Factors / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009186:Myelin Sheath / Q000473:pathology / Q000648:ultrastructure; D009460:Neurologic Examination / Q000379:methods; D009474:Neurons / Q000473:pathology / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism; D011597:Psychomotor Performance; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
D007074:Immunoglobulin G; D007155:Immunologic Factors; D009195:Peroxidase