Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins.
A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.
González-Zamorano
Margarita
M
Departamento de Inmunologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico.
Mendoza-Hernández
Guillermo
G
Xolalpa
Wendy
W
Parada
Cristina
C
Vallecillo
Antonio J
AJ
Bigi
Fabiana
F
Espitia
Clara
C
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D019295:Computational Biology; D003208:Concanavalin A / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008358:Mannose / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D001426:Bacterial Proteins; D006023:Glycoproteins; D003208:Concanavalin A; D008358:Mannose