title: Detection of differential proteomes of human beta-cells during islet-like differentiation using iTRAQ labeling.
abstract: A human beta-cell line, RNAKT-15, was recently established from human pancreatic islets, whereby its differentiation into islet-like beta-cells (islet-like RNAKT-15) increased its expression of insulin 2-fold compared with RNAKT-15 cells. To characterize the differentiation of RNAKT-15 cells into islet-like RNAKT-15, microarray and quantitative proteomics were performed. Our analysis of differential proteomic and mRNA expression has resulted in a greater understanding of the molecular functions that are involved in beta-cell differentiation and insulin synthesis and release.
authors.affiliation: Departments of Biomedical Sciences and Internal Medicine, Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.
authors.forename: Jonghwa
authors.initials: J
authors.lastname: Jin
authors.forename: Jungeun
authors.initials: J
authors.lastname: Park
authors.forename: Kyunggon
authors.initials: K
authors.lastname: Kim
authors.forename: Yup
authors.initials: Y
authors.lastname: Kang
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Park
authors.forename: Jae Hyeon
authors.initials: JH
authors.lastname: Kim
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Heesook
authors.initials: H
authors.lastname: Jun
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002853:Chromatography, Liquid; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000502:physiology*; D007553:Isotope Labeling; D020543:Proteome / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D007328:Insulin; D020543:Proteome; D012333:RNA, Messenger