Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays.
To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.
Lin
Zhenyu
Z
Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aoba, Aramaki, Sendai 980-8579, Japan.
Takahashi
Yasufumi
Y
Murata
Tatsuya
T
Takeda
Michiaki
M
Ino
Kosuke
K
Shiku
Hitoshi
H
Matsue
Tomokazu
T
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000627:Aminophenols / Q000737:chemistry; D002460:Cell Line; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D046228:Microarray Analysis; D008839:Microelectrodes; D010084:Oxidation-Reduction; D011137:Polystyrenes / Q000737:chemistry
D000627:Aminophenols; D011137:Polystyrenes; D000469:Alkaline Phosphatase; C026729:4-aminophenol