Replication of calpain-10 genetic association with carotid intima-media thickness.
OBJECTIVE
Diabetes and atherosclerosis may share common genetic determinants. A prior study in Hispanics found association of haplotypes in the diabetes gene calpain-10 (CAPN10) with carotid artery intima-media thickness (CIMT). This study sought to replicate this association in an independent cohort.


METHODS
Four CAPN10 SNPs were genotyped and haplotypes determined in 487 Hispanic Americans from 143 families ascertained via an index case with hypertension. CIMT was measured from B-mode ultrasound, and glycemic traits quantified from euglycemic clamps. Association of SNPs and haplotypes with CIMT was determined.


RESULTS
The minor alleles of SNP-56 and SNP-63 were associated with increased CIMT in dominant and additive models. The association of haplotype 1112 with increased CIMT was replicated. No associations with fasting insulin, insulin secretion, or insulin sensitivity were observed.


CONCLUSIONS
CAPN10 association with CIMT was replicated, further supporting its role as a common genetic determinant of diabetes and atherosclerosis in Hispanics.
Goodarzi
Mark O
MO
Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA. mark.goodarzi@cshs.org
Taylor
Kent D
KD
Jones
Michelle R
MR
Fang
Belle
B
Guo
Xiuqing
X
Xiang
Anny H
AH
Buchanan
Thomas A
TA
Hodis
Howard N
HN
Raffel
Leslie J
LJ
Rotter
Jerome I
JI
D000328:Adult; D002154:Calpain / Q000235:genetics* / Q000502:physiology; D002339:Carotid Arteries / Q000473:pathology; D015331:Cohort Studies; D003920:Diabetes Mellitus / Q000208:ethnology / Q000235:genetics; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006630:Hispanic or Latino; D006801:Humans; D006973:Hypertension / Q000235:genetics; D007328:Insulin / Q000378:metabolism; D008297:Male; D017539:Tunica Intima / Q000473:pathology*; D017540:Tunica Media / Q000473:pathology*; D014463:Ultrasonography / Q000379:methods
D007328:Insulin; D002154:Calpain; C415197:calpain 10