title: Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis.
abstract: OBJECTIVE
  To explore the association of microbial community structure with the development of eutrophication in a large shallow freshwater lake, Lake Taihu.
  
  
  RESULTS
  The bacterial and archaeal assemblages in sediments of different lake areas were analysed using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA fragments. The bacterial DGGE profiles showed that eutrophied sites, grass-bottom areas and relatively clean sites with a eutrophic (albeit dredged) site are three respective clusters. Fifty-one dominant bacterial DGGE bands were detected and 92 corresponding clones were sequenced, most of which were affiliated with bacterial phylotypes commonly found in freshwater ecosystems. Actinobacteria were detected in the centre of the lake and not at eutrophied sites whereas the opposite was found with respect to Verrucomicrobiales. Twenty-five dominant archaeal DGGE bands were detected and 31 corresponding clones were sequenced, most of which were affiliated with freshwater archaeal phylotypes.
  
  
  CONCLUSIONS
  The bacterial community structures in the sediments of different areas with similar water quality and situation tend to be similar in Taihu Lake.
  
  
  CONCLUSIONS
  This study may expand our knowledge on the relationship between the overall microbial assemblages and the development of eutrophication in the shallow freshwater lake.
authors.affiliation: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: F H
authors.initials: FH
authors.lastname: Liu
authors.forename: G H
authors.initials: GH
authors.lastname: Lin
authors.forename: G
authors.initials: G
authors.lastname: Gao
authors.forename: B Q
authors.initials: BQ
authors.lastname: Qin
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.forename: G P
authors.initials: GP
authors.lastname: Zhao
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhou
authors.forename: J H
authors.initials: JH
authors.lastname: Shen
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005068:Eutrophication / Q000502:physiology; D005618:Fresh Water; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S