Chapter 7. Nonsense-mediated mRNA decay in Caenorhabditis elegans.
The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This chapter describes the development of transgenic reporters as well as the use of genome-wide RNAi and genetic screens to identify novel components of the NMD pathway in the nematode Caenorhabditis elegans.
Longman
Dasa
D
Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, United Kingdom.
Arrisi
Pablo
P
Johnstone
Iain L
IL
Cáceres
Javier F
JF
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D018389:Codon, Nonsense / Q000235:genetics; D034622:RNA Interference / Q000502:physiology; D020871:RNA Stability / Q000235:genetics*
D018389:Codon, Nonsense