Genetic diversity of Trichomonas vaginalis clinical isolates determined by EcoRI restriction fragment length polymorphism of heat-shock protein 70 genes.
Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with EcoRI-digested genomic DNA was used in molecular typing of 129 Trichomonas vaginalis isolates. Results indicate that Trichomonas organisms exhibit considerable polymorphism in their Hsp70 RFLP patterns. Analysis of seven American Type Culture Collection reference strains and 122 clinical isolates, including 84 isolates from Jackson, Mississippi, 18 isolates from Atlanta, Georgia, and 20 isolates from throughout the United States, showed 105 distinct Hsp70 RFLP pattern subtypes for Trichomonas. Phylogenetic analysis of the Hsp70 RFLP data showed that the T. vaginalis isolates were organized into two clonal lineages. These results illustrate the substantial genomic diversity present in T. vaginalis and indicate that a large number of genetically distinct Trichomonas isolates may be responsible for human trichomoniasis in the United States.
Meade
John C
JC
Department of Microbiology, Division of Infectious Diseases, University of Mississippi Medical Center, Jackson, Mississippi 39216-4505, USA. jmeade@microbio.umsmed.edu
de Mestral
Jacqueline
J
Stiles
Jonathan K
JK
Secor
W Evan
WE
Finley
Richard W
RW
Cleary
John D
JD
Lushbaugh
William B
WB
D000818:Animals; D015373:Bacterial Typing Techniques; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D005260:Female; D014644:Genetic Variation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D014247:Trichomonas Vaginitis / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D018840:HSP70 Heat-Shock Proteins; D015246:Deoxyribonuclease EcoRI