title: Development of a sequential injection chromatography (SIC) method for determination of simazine, atrazine, and propazine.
abstract: This paper describes the development of a sequential injection chromatography (SIC) procedure for separation and quantification of the herbicides simazine, atrazine, and propazine exploring the low backpressure of a 2.5 cm long monolithic C(18) column. The separation of the three compounds was achieved in less than 90 s with resolution >1.5 using a mobile phase composed by ACN/1.25 mmol/L acetate buffer (pH 4.5) at the volumetric ratio of 35:65 and flow rate of 40 microL/s. Detection was made at 223 nm using a flow cell with 40 mm of optical path length. The LOD was 10 microg/L for the three triazines and the quantification limits were of 30 microg/L for simazine and propazine and 40 microg/L for atrazine. The sampling frequency is 27 samples per hour, consuming 1.1 mL of ACN per analysis. The proposed methodology was applied to spiked water samples and no statistically significant differences were observed in comparison to a conventional HPLC-UV method. The major metabolites of atrazine and other herbicides did not interfere in the analysis, being eluted from the column either together with the unretained peak, or at retention times well-resolved from the studied compounds.
authors.affiliation: Instituto de Química, Universidade de São Paulo, C.P. 26077, São Paulo, SP, Brazil.
authors.forename: Luciana B O
authors.initials: LB
authors.lastname: Dos Santos
authors.forename: Carlos M C
authors.initials: CM
authors.lastname: Infante
authors.forename: Jorge C
authors.initials: JC
authors.lastname: Masini
mesh_terms: D001280:Atrazine / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D012839:Simazine / Q000032:analysis* / Q000737:chemistry*; D014227:Triazines / Q000032:analysis* / Q000737:chemistry*
chemical_list: D014227:Triazines; D001280:Atrazine; D012839:Simazine; C100043:propazine