title: An E7-based therapeutic vaccine protects mice against HPV16 associated cancer.
abstract: Plant-derived vaccines represent an attractive strategy for cancer immunotherapy due to their relative safety and cost-effectiveness. We evaluated the anti-tumour activity of a Nicotiana benthamiana produced vaccine candidate based on the non-transforming E7 protein of HPV-16 fused to beta-1,3-1,4-glucanase of Clostridium thermocellum. Two doses of vaccine at two week intervals were administered to groups of C57BL/6 mice starting 3 or 6 days after challenge with tumourigenic E7-expressing TC-1* cells. Inhibition of tumour growth and increased survival was observed in both groups treated with vaccine. These data suggest the potential of plants as a platform for producing therapeutic vaccines.
authors.affiliation: Regina Elena Cancer Institute, Laboratory of Virology, Via delle Messi d'Oro 156, 00158 Rome, Italy. venuti@ifo.it
authors.forename: Aldo
authors.initials: A
authors.lastname: Venuti
authors.forename: Silvia
authors.initials: S
authors.lastname: Massa
authors.forename: Vadim
authors.initials: V
authors.lastname: Mett
authors.forename: Laura Dalla
authors.initials: LD
authors.lastname: Vedova
authors.forename: Francesca
authors.initials: F
authors.lastname: Paolini
authors.forename: Rosella
authors.initials: R
authors.lastname: Franconi
authors.forename: Vidadi
authors.initials: V
authors.lastname: Yusibov
mesh_terms: D000818:Animals; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000517:prevention & control*; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000517:prevention & control*; D053918:Papillomavirus Vaccines / Q000627:therapeutic use*; D014026:Nicotiana / Q000235:genetics; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000627:therapeutic use*
chemical_list: D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D014614:Vaccines, Synthetic; C059731:oncogene protein E7, Human papillomavirus type 16