title: Sequence-related amplified polymorphism, an effective molecular approach for studying genetic variation in Fasciola spp. of human and animal health significance.
abstract: In the present study, a recently described molecular approach, namely sequence-related amplified polymorphism (SRAP), which preferentially amplifies ORFs, was evaluated for the studies of genetic variation among Fasciola hepatica, Fasciola gigantica and the "intermediate" Fasciola from different host species and geographical locations in mainland China. Five SRAP primer combinations were used to amplify 120 Fasciola samples after ten SRAP primer combinations were evaluated. The number of fragments amplified from Fasciola samples using each primer combination ranged from 12 to 20, with an average of 15 polymorphic bands per primer pair. Fifty-nine main polymorphic bands were observed, ranging in size from 100 to 2000 bp, and SRAP bands specific to F. hepatica or F. gigantica were observed. SRAP fragments common to F. hepatica and the "intermediate" Fasciola, or common to F. gigantica and the "intermediate" Fasciola were identified, excised and confirmed by PCR amplification of genomic DNA using primers designed based on sequences of these SRAP fragments. Based on SRAP profiles, unweighted pair-group method with arithmetic averages clustering algorithm categorized all of the examined representative Fasciola samples into three groups, representing the F. hepatica, the "intermediate" Fasciola, or the F. gigantica. These results demonstrated the usefulness of the SRAP technique for revealing genetic variability between F. hepatica, F. gigantica and the "intermediate" Fasciola, and also provided genomic evidence for the existence of the "intermediate" Fasciola between F. hepatica and F. gigantica. This technique provides an alternative and a useful tool for the genetic characterization and studies of genetic variability in parasites.
authors.affiliation: South China Agricultural University, Guangdong Province, P. R. China.
authors.forename: Qiao-Yan
authors.initials: QY
authors.lastname: Li
authors.forename: Shi-Juan
authors.initials: SJ
authors.lastname: Dong
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Zhang
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Lin
authors.forename: Chun-Ren
authors.initials: CR
authors.lastname: Wang
authors.forename: De-Xing
authors.initials: DX
authors.lastname: Qian
authors.forename: Zhao-Rong
authors.initials: ZR
authors.lastname: Lun
authors.forename: Hui-Qun
authors.initials: HQ
authors.lastname: Song
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005209:Fasciola / Q000235:genetics*; D005210:Fasciola hepatica / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers