Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120.
Cyanobacteriochromes form a recently defined superfamily of tetrapyrrole-based photoreceptors that are distantly related to conventional red/far-red photoreceptor phytochromes. Among these molecules, AnPixJ from Anabaena sp. PCC 7120 is a novel photoreceptor that shows reversible photoconversion between green-absorbing and red-absorbing forms, which is in contrast to the properties of conventional phytochromes. In order to better understand the structural basis of this unique photoconversion mechanism, the chromophore-binding domain of AnPixJ (AnPixJ-GAF2) was heterologously overproduced and purified, and crystallization of both forms was attempted. Blue crystals of the red-absorbing form of AnPixJ-GAF2 were successfully obtained; they belonged to space group P4(3)2(1)2 and contained one monomer per asymmetric unit. Diffraction data were collected to a resolution of 1.8 A using synchrotron-radiation beamline BL-5A at the Photon Factory.
Narikawa
Rei
R
Department of Life Sciences, Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan. narikawa@bio.c.u-tokyo.ac.jp
Muraki
Norifumi
N
Shiba
Tomoo
T
Ikeuchi
Masahiko
M
Kurisu
Genji
G
D017033:Anabaena / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000378:metabolism; D052980:Phycobilins / Q000096:biosynthesis; D010798:Phycocyanin / Q000096:biosynthesis; D010834:Phytochrome / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D052980:Phycobilins; D010798:Phycocyanin; D010834:Phytochrome; C011069:phycocyanobilin