title: Association of resistance to avian coccidiosis with single nucleotide polymorphisms in the zyxin gene.
abstract: Our previous genetic studies demonstrated that resistance to avian coccidiosis is linked with microsatellite markers LEI0071 and LEI0101 on chromosome 1. In this study, the associations between parameters of resistance to coccidiosis and single nucleotide polymorphisms (SNP) in 3 candidate genes located between LEI0071 and LEI0101 [zyxin, CD4, and tumor necrosis factor receptor super family 1A (TNFRSF1A)] were determined. The SNP were genotyped in 24 F(1) generation and 290 F(2) generation animals. No SNP were identified in the TNFRSF1A gene, whereas 10 were located in the zyxin gene and 4 in the CD4 gene. At various times following experimental infection of the F(2) generation with Eimeria maxima, BW, fecal oocyst shedding, and plasma levels of carotenoid, nitrite plus nitrate (NO(2)(-) + NO(3)(-)), and interferon-gamma (IFN-gamma) were measured as parameters of resistance. Single marker and haplotype-based tests were applied to determine the associations between the 14 SNP and the parameters of coccidiosis resistance. None of the CD4 SNP were correlated with disease resistance. However, by single marker association, several of the zyxin SNP were significantly associated with carotenoid or NO(2)(-) + NO(3)(-) concentrations. These were the SNP at nucleotide 149 associated with carotenoid at d 3 postinfection (PI), nucleotide 187 with carotenoid at d 6 and 9 PI, and nucleotide 159 with carotenoid between d 3 and 9 PI. In addition, the zyxin SNP at nucleotide 191 was significantly associated with increased levels of NO(2)(-) + NO(3)(-) at d 3 PI. By haplotype association, the zyxin SNP also were found to be highly associated with NO(2)(-) + NO(3)(-) at d 3 PI and increased IFN-gamma at d 6 PI. These results suggest that zyxin is a candidate gene potentially associated with increased resistance to experimental avian coccidiosis.
authors.affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, USDA, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov
authors.forename: Y H
authors.initials: YH
authors.lastname: Hong
authors.forename: E-S
authors.initials: ES
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: E P
authors.initials: EP
authors.lastname: Lillehoj
authors.forename: K-D
authors.initials: KD
authors.lastname: Song
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D015704:CD4 Antigens / Q000235:genetics; D002645:Chickens; D003048:Coccidiosis / Q000235:genetics / Q000662:veterinary*; D004539:Eimeria; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008297:Male; D008667:Metalloproteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011201:Poultry Diseases / Q000235:genetics* / Q000469:parasitology; D060589:Zyxin
chemical_list: D030161:Avian Proteins; D015704:CD4 Antigens; D008667:Metalloproteins; D060589:Zyxin