title: Detection of LP2086 on the cell surface of Neisseria meningitidis and its accessibility in the presence of serogroup B capsular polysaccharide.
abstract: The outer membrane protein LP2086, a human factor H binding protein, is undergoing clinical trials as a vaccine against invasive serogroup B meningococcal (MnB) disease. As LP2086 is a surface protein, expression of capsular polysaccharide could potentially limit accessibility of anti-LP2086 antibodies to LP2086 expressed on the surface of bacteria. To determine whether variability in expression levels of the serogroup B capsule (Cap B) might interfere with accessibility of anti-LP2086 antibody binding to LP2086, we evaluated the ability of anti-Cap B and anti-LP2086 antibodies to bind to the surface of 1263 invasive clinical MnB strains by flow cytometry. One of the anti-LP2086 monoclonal antibodies used recognizes virtually all LP2086 sequence variants. Our results show no correlation between the amount of Cap B expressed and the binding of anti-LP2086 antibodies. Furthermore, the susceptibility of MnB bacteria to lysis by anti-LP2086 immune sera was independent of the level of Cap B expressed. The data presented in this paper demonstrates that Cap B does not interfere with the binding of antibodies to LP2086 expressed on the outer membrane of MnB clinical isolates.
authors.affiliation: Wyeth Research, 401 N. Middletown Rd., Pearl River, NY 10965, United States.
authors.forename: Lisa K
authors.initials: LK
authors.lastname: McNeil
authors.forename: Ellen
authors.initials: E
authors.lastname: Murphy
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhao
authors.forename: Stephen
authors.initials: S
authors.lastname: Guttmann
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Harris
authors.forename: Adrienne A
authors.initials: AA
authors.lastname: Scott
authors.forename: Cuiwen
authors.initials: C
authors.lastname: Tan
authors.forename: Michelle
authors.initials: M
authors.lastname: Mack
authors.forename: Ida
authors.initials: I
authors.lastname: DaSilva
authors.forename: Kristin
authors.initials: K
authors.lastname: Alexander
authors.forename: Kathryn
authors.initials: K
authors.lastname: Mason
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Jiang
authors.forename: Duzhang
authors.initials: D
authors.lastname: Zhu
authors.forename: Terri L
authors.initials: TL
authors.lastname: Mininni
authors.forename: Gary W
authors.initials: GW
authors.lastname: Zlotnick
authors.forename: Susan K
authors.initials: SK
authors.lastname: Hoiseth
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Jones
authors.forename: Michael W
authors.initials: MW
authors.lastname: Pride
authors.forename: Kathrin U
authors.initials: KU
authors.lastname: Jansen
authors.forename: Annaliesa S
authors.initials: AS
authors.lastname: Anderson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001770:Blood Bactericidal Activity; D005260:Female; D006801:Humans; D009345:Neisseria meningitidis / Q000737:chemistry / Q000276:immunology*; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology*; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C484699:factor H-binding protein, Neisseria meningitidis