title: High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ tris-nitrilotriacetic acid quantum dot conjugates.
abstract: Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for QD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates tris-nitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using QD-trisNTA, we demonstrate two-color QD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein-protein interactions at the single molecule level.
authors.affiliation: Université de Rennes, Sciences Chimiques de Rennes, CNRS UMR 6226, Campus de Beaulieu, 35042 Rennes Cedex, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Roullier
authors.forename: Samuel
authors.initials: S
authors.lastname: Clarke
authors.forename: Changjiang
authors.initials: C
authors.lastname: You
authors.forename: Fabien
authors.initials: F
authors.lastname: Pinaud
authors.forename: G Géraldine
authors.initials: GG
authors.lastname: Gouzer
authors.forename: Dirk
authors.initials: D
authors.lastname: Schaible
authors.forename: Valérie
authors.initials: V
authors.lastname: Marchi-Artzner
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry; D002448:Cell Adhesion; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D011092:Polyethylene Glycols; D045663:Quantum Dots; D017471:Receptors, Interferon / Q000378:metabolism; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D019187:Cadmium Compounds; C485182:Cyan Fluorescent Protein; D017471:Receptors, Interferon; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D006639:Histidine; D009532:Nickel; C058667:cadmium selenide; D009571:Nitrilotriacetic Acid; C031238:zinc sulfide