Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous-organic medium.
To enhance penicillin acylase (PA) performance, it was immobilized in mesocellular silica foams (MCFs) depended on macromolecular crowding and applied to catalysis in non-aqueous medium. Ficoll 70, dextran 10,000, dextran 40,000 and bovine serum albumin were co-assembled with PA. It was observed that specific activity of PA assembled in MCFs with dextran 10,000 in 80% cyclohexane (v/v) was 233.2 U/mg, 200% as that of PA assembled in MCFs in 80% cyclohexane and 323.5% as that of free PA in full aqueous medium. As content of alkane increased, activity of PA in MCFs with macromolecules varied slightly. In addition, PA co-immobilized with dextran 10 in MCFs retained 58.2% of its initial activity after heating at 50 degrees C for 4 h, 1.2 times higher than that of PA immobilized alone in MCFs. The results showed that macromolecular crowding was favorable for immobilization of PA and its catalysis in suitable aqueous-organic medium.
Xue
Jianyue
J
Department of Chemistry and Materials Science, Chaohu University, 238000 Chaohu, People's Republic of China.
Wang
Anming
A
Zhou
Cheng
C
Shen
Shubao
S
D000818:Animals; D001410:Bacillus megaterium / Q000201:enzymology; D002384:Catalysis; D002417:Cattle; D003911:Dextrans; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005362:Ficoll; D066298:In Vitro Techniques; D046912:Multiprotein Complexes; D010399:Penicillin Amidase / Q000737:chemistry* / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D012996:Solutions; D013816:Thermodynamics; D014867:Water
D003911:Dextrans; D004800:Enzymes, Immobilized; D046912:Multiprotein Complexes; D012996:Solutions; D014867:Water; D005362:Ficoll; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D010399:Penicillin Amidase