PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia.
In this study, polymerase chain reaction (PCR) was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 (61.4%) were serologically Toxoplasma-IgG positive meanwhile only 16/43 (37.2%) were approved to be positive by PCR. A total of 22 samples (31.6%) were positive by PCR, but only 16 of them were positive by ELISA. So, Tox-IgG indicated catching Toxoplasma infection but not enough with immune modulation to determine acute case, while PCR besides, being valuable in diagnosis showed Toxoplasma parasitemia.
Tonkal
Abdulkader M D
AM
Department of Medical Parasitology, Faculty of Medicine King Abdul Aziz University, Jeddah, Saudi Arabia.
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016054:DNA, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis; D015203:Reproducibility of Results; D012529:Saudi Arabia; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan