title: Mitochondria isolated from local market potato tubers contain L-lactate dehydrogenase in an inactive state.
abstract: In order to gain some insight into metabolism of mitochondria isolated from materials subjected to storage treatments, we compared mitochondria isolated from potato tubers grown and stored in the post-harvest without any chemicals (N-PTM), and tubers, from local market, treated for commercial purpose (T-PTM) with respect to the L-lactate metabolism. Although no oxygen consumption due to L-lactate was found in T-PTM, L-lactate dehydrogenase existence was shown as immunologically investigated. Consistently, no L-lactate dehydrogenase activity was detected. Contrarily, N-PTM proved to metabolize externally added L-lactate, with oxygen consumption and intramitochondrial pyridine nucleotide reduction. All together these findings show that commercial treatments of foodstuffs could result in changes in their metabolism.
authors.affiliation: Dipartimento di Scienze per la Salute, Università del Molise, Campobasso, Italy. paventi@unimol.it
authors.forename: Gianluca
authors.initials: G
authors.lastname: Paventi
authors.forename: Roberto
authors.initials: R
authors.lastname: Pizzuto
authors.forename: Gabriella
authors.initials: G
authors.lastname: Chieppa
authors.forename: Salvatore
authors.initials: S
authors.lastname: Passarella
mesh_terms: D005470:Fluorometry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011198:Solanum tuberosum / Q000201:enzymology* / Q000378:metabolism
chemical_list: D007773:Lactates; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D007770:L-Lactate Dehydrogenase