[Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD].
OBJECTIVE
To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.


METHODS
HPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them.


RESULTS
Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol x L(-1) HCl, and furthermore their remained percentage was up to 98% and 103%.


CONCLUSIONS
The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol x L(-1) HCl in the solution.
Wang
Qi
Q
School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhang
Yu-jie
YJ
Li
Wei-feng
WF
Yang
Jie
J
Jiang
Hao
H
Sun
Wan-jing
WJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000097:blood* / Q000493:pharmacokinetics*; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D044950:Flavanones; D005419:Flavonoids; C038044:baicalin; C006680:baicalein