title: Evidence of intra-familial transmission of Helicobacter pylori by PCR-based RAPD fingerprinting in Bangladesh.
abstract: Helicobacter pylori is a genetically diverse bacterial species, which has facilitated adaptation to new hosts and persists worldwide. The main objective of this study was to explore intra-familial transmission of H. pylori in Bangladesh. We characterized H. pylori in 35 families including 138 family members using random amplified polymorphic DNA (RAPD) fingerprinting. Forty-six percent of H. pylori isolated from the mother shared a related genotype with strains isolated from their children. Twenty-nine percent of H. pylori isolates of the mother are related to the youngest children. Only 6% of the parents shared related genotype of H. pylori. These findings suggest that mother-to-child transmission occurs in early childhood and is the most probable route of transmission of H. pylori in Bangladesh.
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh. snahar@icddrb.org
authors.forename: S
authors.initials: S
authors.lastname: Nahar
authors.forename: K M K
authors.initials: KM
authors.lastname: Kibria
authors.forename: Md E
authors.initials: ME
authors.lastname: Hossain
authors.forename: J
authors.initials: J
authors.lastname: Sultana
authors.forename: S A
authors.initials: SA
authors.lastname: Sarker
authors.forename: L
authors.initials: L
authors.lastname: Engstrand
authors.forename: P K
authors.initials: PK
authors.lastname: Bardhan
authors.forename: M
authors.initials: M
authors.lastname: Rahman
authors.forename: H P
authors.initials: HP
authors.lastname: Endtz
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D001459:Bangladesh / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D005192:Family Health; D005260:Female; D016481:Helicobacter Infections / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial