Expression of cell cycle biomarkers and telomere length in papillary thyroid carcinoma: a comparative study between radiation-associated and spontaneous cancers.
OBJECTIVE
Radiation exposure during childhood is the only well-established risk factor for papillary thyroid carcinoma (PTC). To better define the biologic profile of radiation-induced and sporadic PTC, we compared in these two groups of PTC the expression of cell cycle regulatory proteins and telomere length.


METHODS
Cell cycle markers (cyclin A, B1, D1, E, and Ki67) were evaluated on 100 PTC specimens (26 radiation-induced and 74 sporadic PTCs). The expression of cell cycle regulators was studied using immunohistochemistry; telomere length heterogeneity was studied using in situ hybridization in a subset of 16 formalin-fixed samples (8 radiation-induced and 8 sporadic PTCs).


RESULTS
At multivariate analysis, only cytoplasmic cyclin E staining was overexpressed in sporadic cases (P = 0.006). The other cell cycle markers and telomere length did not differ significantly between sporadic PTC and radiation-induced PTC.


CONCLUSIONS
These markers cannot be used to differentiate radiation-induced from sporadic PTCs.
Achille
Mihaela
M
Laboratoire de Radiobiologie et Oncologie, DSV/DRR/Commissariat à l'Energie Atomique, Villejuif, France.
Boukheris
Houda
H
Caillou
Bernard
B
Talbot
Monique
M
de Vathaire
Florent
F
Sabatier
Laure
L
Desmaze
Chantal
C
Schlumberger
Martin
M
Soria
Jean-Charles
JC
D018263:Adenocarcinoma, Follicular / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D016615:Telomere / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis; D055815:Young Adult
D018797:Cell Cycle Proteins