The Streptococcus equi prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase that is produced during equine infection.
Streptococcus equi causes equine 'strangles'. Hyaluronate lyases, which degrade connective tissue hyaluronan and chondroitins, are thought to facilitate streptococcal invasion of the host. However, prophage-encoded hyaluronate lyases are hyaluronan-specific and are thought to be primarily involved in the degradation of the hyaluronan capsule of streptococci during bacteriophage infection. To understand the role of prophage-encoded hyaluronate lyases further, we have biochemically characterized such a hyaluronate lyase, SEQ2045 from S. equi, and have shown that it is produced during equine infection. Prophage-encoded hyaluronan-specific hyaluronate lyases may therefore play a more direct role in disease pathogenesis than previously thought.
Lindsay
Anna-Marie
AM
School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
Zhang
Meng
M
School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
Mitchell
Zoe
Z
Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Holden
Matthew T G
MTG
The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
Waller
Andrew S
AS
Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Sutcliffe
Iain C
IC
School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
Black
Gary W
GW
School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
D000595:Amino Acid Sequence; D000818:Animals; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D006820:Hyaluronic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D039002:Prophages / Q000201:enzymology*; D016415:Sequence Alignment; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D017108:Streptococcus Phages / Q000201:enzymology*; D018502:Streptococcus equi / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins; D006820:Hyaluronic Acid; D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase