title: Proteomic detection of proteins involved in perchlorate and chlorate metabolism.
abstract: Mass spectrometry and a time-course cell lysis method were used to study proteins involved in perchlorate and chlorate metabolism in pure bacterial cultures and environmental samples. The bacterial cultures used included Dechlorosoma sp. KJ, Dechloromonas hortensis, Pseudomonas chloritidismutans ASK-1, and Pseudomonas stutzeri. The environmental samples included an anaerobic sludge enrichment culture from a sewage treatment plant, a sample of a biomass-covered activated carbon matrix from a bioreactor used for treating perchlorate-contaminated drinking water, and a waste water effluent sample from a paper mill. The approach focused on detection of perchlorate (and chlorate) reductase and chlorite dismutase proteins, which are the two central enzymes in the perchlorate (or chlorate) reduction pathways. In addition, acetate-metabolizing enzymes in pure bacterial samples and housekeeping proteins from perchlorate (or chlorate)-reducing microorganisms in environmental samples were also identified.
authors.affiliation: Environmental Biotechnology Institute, University of Idaho, Moscow, ID 83844-1052, USA.
authors.forename: Reema
authors.initials: R
authors.lastname: Bansal
authors.forename: Lee A
authors.initials: LA
authors.lastname: Deobald
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Crawford
authors.forename: Andrzej J
authors.initials: AJ
authors.lastname: Paszczynski
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis*; D018533:Biomass; D019149:Bioreactors; D002704:Chlorates / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D010088:Oxidoreductases / Q000032:analysis; D010472:Perchlorates / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011549:Pseudomonas / Q000201:enzymology; D043362:Rhodocyclaceae / Q000201:enzymology; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid
chemical_list: D001426:Bacterial Proteins; D002704:Chlorates; D010472:Perchlorates; D012722:Sewage; D010088:Oxidoreductases; C402794:chlorite dismutase; C021777:chlorate reductase; C494474:perchlorate