title: Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic Acid-conjugated dendrimers.
abstract: Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.
authors.affiliation: Programs in Applied Physics, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Landmark
authors.forename: Stassi
authors.initials: S
authors.lastname: Dimaggio
authors.forename: Jesse
authors.initials: J
authors.lastname: Ward
authors.forename: Christopher
authors.initials: C
authors.lastname: Kelly
authors.forename: Stefan
authors.initials: S
authors.lastname: Vogt
authors.forename: Seungpyo
authors.initials: S
authors.lastname: Hong
authors.forename: Alina
authors.initials: A
authors.lastname: Kotlyar
authors.forename: Andrzej
authors.initials: A
authors.lastname: Myc
authors.forename: Thommey P
authors.initials: TP
authors.lastname: Thomas
authors.forename: James E
authors.initials: JE
authors.lastname: Penner-Hahn
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
authors.forename: Mark M Banaszak
authors.initials: MM
authors.lastname: Holl
authors.forename: Bradford G
authors.initials: BG
authors.lastname: Orr
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000493:pharmacokinetics; D003460:Crystallization / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D005290:Ferric Compounds / Q000493:pharmacokinetics; D005492:Folic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D008422:Materials Testing; D008968:Molecular Conformation; D050997:Nanomedicine / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D013499:Surface Properties
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D005290:Ferric Compounds; D046911:Macromolecular Substances; C000499:ferric oxide; D005492:Folic Acid