Expression of a novel OSPGYRP (rice proline-, glycine- and tyrosine-rich protein) gene, which is involved in vesicle trafficking, enhanced cold tolerance in E. coli.
A novel OSPGYRP gene encoding a rice proline-, glycine- and tyrosine-rich protein was isolated from cold-stress treated rice seedlings using suppression subtractive hybridization. Both amino acid sequence analysis and subcellular localization confirm that OsPGYRP is a novel protein involved in vesicle trafficking. The expression of the OSPGYRP gene was induced by cold, salt, and osmotic stress. In addition, expression of the OSPGYRP gene in E. coli increased the resistance to cold stress. These results show that OsPGYRP is a novel protein involved in vesicle trafficking and plays an important role in plant adaptation to stress.
Li
Hui
H
Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China.
Yang
Jing
J
Wang
Yayu
Y
Chen
Zhijun
Z
Tu
Sansi
S
Feng
Lingling
L
Zhu
Yingguo
Y
Li
Yangsheng
Y
D000595:Amino Acid Sequence; D003080:Cold Temperature; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D008969:Molecular Sequence Data; D012275:Oryza; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D016415:Sequence Alignment; D013312:Stress, Physiological
D010940:Plant Proteins