title: The Streptococcus equi prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase that is produced during equine infection.
abstract: Streptococcus equi causes equine 'strangles'. Hyaluronate lyases, which degrade connective tissue hyaluronan and chondroitins, are thought to facilitate streptococcal invasion of the host. However, prophage-encoded hyaluronate lyases are hyaluronan-specific and are thought to be primarily involved in the degradation of the hyaluronan capsule of streptococci during bacteriophage infection. To understand the role of prophage-encoded hyaluronate lyases further, we have biochemically characterized such a hyaluronate lyase, SEQ2045 from S. equi, and have shown that it is produced during equine infection. Prophage-encoded hyaluronan-specific hyaluronate lyases may therefore play a more direct role in disease pathogenesis than previously thought.
authors.affiliation: School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Anna-Marie
authors.initials: AM
authors.lastname: Lindsay
authors.affiliation: School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
authors.forename: Zoe
authors.initials: Z
authors.lastname: Mitchell
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
authors.forename: Matthew T G
authors.initials: MTG
authors.lastname: Holden
authors.affiliation: Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Waller
authors.affiliation: School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Iain C
authors.initials: IC
authors.lastname: Sutcliffe
authors.affiliation: School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Gary W
authors.initials: GW
authors.lastname: Black
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D006820:Hyaluronic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D039002:Prophages / Q000201:enzymology*; D016415:Sequence Alignment; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D017108:Streptococcus Phages / Q000201:enzymology*; D018502:Streptococcus equi / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D006820:Hyaluronic Acid; D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase