Antimicrobial activity of the iron-sulfur nitroso compound Roussin's black salt [Fe4S3(NO)7] on the hyperthermophilic archaeon Pyrococcus furiosus.
The iron-sulfur nitroso compound [Fe(4)S(3)(NO)(7)](-) is a broad-spectrum antimicrobial agent that has been used for more than 100 years to combat pathogenic anaerobes. Known as Roussin's black salt (RBS), it contains seven moles of nitric oxide, the release of which was always assumed to mediate its cytotoxicity. Using the hyperthermophilic archaeon Pyrococcus furiosus, it is demonstrated through growth studies, membrane analyses, and scanning electron microscopy that nitric oxide does not play a role in RBS toxicity; rather, the mechanism involves membrane disruption leading to cell lysis. Moreover, insoluble elemental sulfur (S(0)), which is reduced by P. furiosus to hydrogen sulfide, prevents cell lysis by RBS. It is proposed that S(0) also directly interacts with the membranes of P. furiosus during its transfer into the cell, ultimately for reduction by a cytosolic NADPH sulfur reductase. RBS is proposed to be a new class of inorganic antimicrobial agent that also has potential use as an inert cell-lysing agent.
Hamilton-Brehm
Scott D
SD
Department of Biochemistry and Molecular Biology, Davison Life Sciences Complex, University of Georgia, Athens, GA 30602-7229, USA.
Schut
Gerrit J
GJ
Adams
Michael W W
MW
D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D004202:Disinfectants / Q000494:pharmacology*; D006862:Hydrogen Sulfide / Q000378:metabolism; D058085:Iron Compounds / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D009603:Nitroso Compounds / Q000494:pharmacology*; D020027:Pyrococcus furiosus / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D013455:Sulfur / Q000378:metabolism
D004202:Disinfectants; D058085:Iron Compounds; D009603:Nitroso Compounds; C078952:Roussin's Black Salt; D013455:Sulfur; D006862:Hydrogen Sulfide