title: Expression of MMPs and TIMPs family in human ACL and MCL fibroblasts.
abstract: The human ACL (anterior cruciate ligament) is susceptible to injury but has poor healing response, whereas an injured MCL (medial collateral ligament) can be repaired relatively well. Since MMPs (matrix metalloproteases) and TIMPs (tissue inhibitor of metalloproteases) are involved in this tissue remodeling process, investigation of different response of MMPs and TIMPs family in ACL and MCL fibroblasts might lead to understanding the differential matrix remodeling process as well as their different healing ability. The first step would be determination of whether these tissue remodeling effectors are present in ligaments. In this study, we designed primers for real-time RT-PCR and determined the expression of MMPs and TIMPs family in ACL and MCL fibroblasts with synovium as a positive control. Semiquantitative RT-PCR revealed that multiple MMPs and TIMPs expressed in human ACL and MCL fibroblasts except MMP-8, 10, 12, 13, 15, 16, 20, and 26. MMP-7 was present in MCL but not in ACL fibroblast. Quantitative real-time RT-PCR showed that mRNA levels of MMP-1, 2, 14, 17, 23A, and 23B and TIMP-4 are significantly higher in MCL than in ACL fibroblasts. However, MMP-3 is higher in ACL than in MCL fibroblasts. We conclude that numerous MMPs and TIMPs family members that are differentially expressed in ACL and MCL might be involved in the differential matrix remodeling process as well as the differential healing ability of ACL and MCL.
authors.affiliation: 111 Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Tang
authors.forename: Ruyue
authors.initials: R
authors.lastname: Xue
authors.forename: Yequan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: K L Paul
authors.initials: KL
authors.lastname: Sung
mesh_terms: D016118:Anterior Cruciate Ligament / Q000166:cytology*; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D017888:Medial Collateral Ligament, Knee / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases