title: Genome-wide validation of Magnaporthe grisea gene structures based on transcription evidence.
abstract: Accurate cDNA data is useful to validate gene structures in a genome. We sequenced 35189 expressed sequence tags (ESTs) obtained from the highly destructive rice blast fungus, Magnaporthe grisea. Our custom-made computational programs mapped these ESTs on the M. grisea genome sequence, and reconstructed gene structures as well as protein-coding regions. As a result, we predicted 4480 protein-coding sequences, which were more accurate than ab initio predictions. Moreover, cross-species comparisons suggested that our predicted proteins were nearly complete. The cDNA clones obtained in this study will be important for further experimental studies. Our genome annotation is available at http://www.mg.dna.affrc.go.jp/.
authors.affiliation: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Numa
authors.forename: Marie
authors.initials: M
authors.lastname: Nishimura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanamori
authors.forename: Ching-Chia
authors.initials: CC
authors.lastname: Yang
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nagamura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Itoh
mesh_terms: D020029:Base Pairing; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D030541:Databases, Genetic; D005091:Exons; D020224:Expressed Sequence Tags; D015723:Gene Library; D005821:Genetic Techniques; D016681:Genome, Fungal; D007438:Introns; D020082:Magnaporthe / Q000235:genetics*; D016366:Open Reading Frames; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal