title: Fe3O4/SiO2-g-PSStNa polymer nanocomposite microspheres (PNCMs) from a surface-initiated atom transfer radical polymerization (SI-ATRP) approach for pectinase immobilization.
abstract: Polymer nanocomposite microspheres (PNCMs) as solid supports can improve the efficiency of immobilized enzymes by reducing diffusional limitation as well as by increasing the surface area per mass unit. In this work, pectinase was immobilized on Fe(3)O(4)/SiO2-g-poly(PSStNa) nanocomposite microspheres by covalent attachment. Biochemical studies showed an improved storage stability of the immobilized pectinase as well as enhanced performance at higher temperatures and over a wider pH range. The immobilized enzyme retained >50% of its initial activity over 30 days, and the optimum temperature and pH also increased to the ranges of 50-60 degrees C and 3.0-4.7, respectively. The kinetics of a model reaction catalyzed by the immobilized pectinase was finally investigated by the Michaelis-Menten equation. The PSStNa support presents a very simple, mild, and time-saving process for enzyme immobilization, and this strategy of immobilizing pectinase also makes use of expensive enzymes economically viable, strengthening repeated use of them as catalysts following their rapid and easy separation with a magnet.
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry (Shaanxi Normal University), Ministry of Education, School of Chemistry and Materials Science, Xi'an 710062, China. zhllei@snnu.edu.cn
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Lei
authors.forename: Na
authors.initials: N
authors.lastname: Ren
authors.forename: Yanli
authors.initials: Y
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Mu
mesh_terms: D055598:Chemical Phenomena; D048271:Chitosan; D004800:Enzymes, Immobilized; D005290:Ferric Compounds / Q000737:chemistry*; D007700:Kinetics; D008863:Microspheres; D053761:Nanocomposites / Q000737:chemistry*; D011096:Polygalacturonase / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D011108:Polymers; D013451:Sulfonic Acids; C000499:ferric oxide; D012822:Silicon Dioxide; D048271:Chitosan; D011096:Polygalacturonase; C077114:styrenesulfonic acid polymer