title: Generation of Vibrio anguillarum ghost by coexpression of PhiX 174 lysis E gene and staphylococcal nuclease A gene.
abstract: Vibrio anguillarum ghosts (VAG) were generated, for the first time, using a conjugation vector containing a ghost bacteria inducing cassette, pRK-lambdaP(R)-cI-Elysis, in which the expression of PhiX174 lysis gene E was controlled by the P ( R )/cI regulatory system of lambda phage. By scanning electron microscopy, holes ranging 80-200 nm in diameter were observed in the VAG. To avoid the presence of bacterial genomic DNA and an antibiotic resistance gene in the final VAG product, we constructed a new dual vector, pRK-lambdaP(R)-cI-E-SNA, containing the E-mediated lysis cassette and the staphylococcal nuclease A (SNA)-mediated DNA degradation cassette, and generated safety-enhanced VAG for use as a fish vaccine.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Pusan, Korea.
authors.forename: Se Ryun
authors.initials: SR
authors.lastname: Kwon
authors.forename: Yue Jai
authors.initials: YJ
authors.lastname: Kang
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Lee
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Lee
authors.forename: Yoon Kwon
authors.initials: YK
authors.lastname: Nam
authors.forename: Sung Koo
authors.initials: SK
authors.lastname: Kim
authors.forename: Ki Hong
authors.initials: KH
authors.lastname: Kim
mesh_terms: D001428:Bacterial Vaccines / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008836:Micrococcal Nuclease / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000166:cytology* / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001428:Bacterial Vaccines; D008836:Micrococcal Nuclease; D014764:Viral Proteins; C052951:3 protein, Bacillus phage Nf