Structure of the lipopolysaccharide core of Vibrio vulnificus type strain 27562.
The structure of the lipopolysaccharide core of Vibrio vulnificus type strain 27562 is presented. LPS hydrolysis gave two oligosaccharides, OS-1 and OS-2, as well as lipid A. NMR spectroscopic data corresponded to the presence of one Kdo residue, one beta-glucopyranose, three heptoses, one glyceric acid, one acetate, three PEtN, and one 5,7-diacylamido-3,5,7,9-tetradeoxynonulosonic acid residue (pseudaminic acid, Pse) in OS1. OS2 differed form OS 1 by the absence of glyceric acid, acetate, and Pse residues. Lipid A was analyzed for fatty acid composition and the following fatty acids were found: C14:0, C12:0-3OH, C16:0, C16:1, C14:0-3OH, C18:0, C18:1 in a ratio of 1:3:3:1:2.5:0.6:0.8.
Vinogradov
Evguenii
E
Institute for Biological Sciences, National Research Council, Ont., Canada.
Wilde
Caroline
C
Anderson
Erin M
EM
Nakhamchik
Alina
A
Lam
Joseph S
JS
Rowe-Magnus
Dean A
DA
D000107:Acetylation; D002240:Carbohydrate Sequence; D005227:Fatty Acids / Q000737:chemistry; D015151:Immunoblotting; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D041261:Vibrio vulnificus / Q000737:chemistry*
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D009844:Oligosaccharides