The archetype gamma-class carbonic anhydrase (Cam) contains iron when synthesized in vivo.
A recombinant protein overproduction system was developed in Methanosarcina acetivorans to facilitate biochemical characterization of oxygen-sensitive metalloenzymes from strictly anaerobic species in the Archaea domain. The system was used to overproduce the archetype of the independently evolved gamma-class carbonic anhydrase. The overproduced enzyme was oxygen sensitive and had full incorporation of iron instead of zinc observed when overproduced in Escherichia coli. This, the first report of in vivo iron incorporation for any carbonic anhydrase, supports the need to reevaluate the role of iron in all classes of carbonic anhydrases derived from anaerobic environments.
Macauley
Sheridan R
SR
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA.
Zimmerman
Sabrina A
SA
Apolinario
Ethel E
EE
Evilia
Caryn
C
Hou
Ya-Ming
YM
Ferry
James G
JG
Sowers
Kevin R
KR
D019843:Archaeal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D002256:Carbonic Anhydrases / Q000096:biosynthesis* / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D007501:Iron / Q000378:metabolism*; D008667:Metalloproteins / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000235:genetics; D017020:Methanosarcina / Q000201:enzymology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification
D019843:Archaeal Proteins; D008667:Metalloproteins; D011994:Recombinant Proteins; D007501:Iron; D002256:Carbonic Anhydrases