[Multiplex ligation-dependent probe amplification and its application].
Multiplex ligation-dependent probe amplification (MLPA) is a semiquantitative analysis based on polymerase chain reaction (PCR). It possesses many advantages such as high efficiency, simple operation, low cost and has been wildly applied in researches of diseases associated with copy number variation, point mutation and methylation. Recently, MLPA is combined with DNA chip to become a real high-throughput method and get great improvement in reliability. Here, the progresses of methods and application of MLPA, as well as its limitations are reviewed.
Zhou
Da-wen
DW
Shanghai Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai, 200040 PR China. zdwmail2006@yahoo.com.cn
Ren
Zhao-rui
ZR
D019175:DNA Methylation; D015342:DNA Probes / Q000032:analysis* / Q000235:genetics*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes