title: Influence of phosphorus on biofilm formation in model drinking water distribution systems.
abstract: OBJECTIVE
  This study investigated the effects of phosphorus on biofilm formation via annular reactor systems in terms of biofilm cell growth, exopolysaccharide (EPS) production, biofilm structure and cell metabolic potential.
  
  
  RESULTS
  Drinking water biofilms were developed in annular reactors with supplement of carbon and different levels of phosphorus. The biofilm formation was monitored over a period of 30 days. Biofilm related parameters were examined by various methods, which included heterotrophic plate count, total carbohydrate content, confocal laser scanning microscopy and GN2 microplate assay. Our results showed that phosphorus addition can promote the biofilm cell growth (cell count increased about 1 log with addition of 30 and 300 microg l(-1) of phosphorus). However, the addition of 30 and 300 microg l(-1) of phosphorus caused 81% and 77% decrease in EPS production, respectively. The results of biofilm structure analysis showed that the addition of 30 and 300 microg l(-1) of phosphorus can induce thicker and less homogeneous biofilms with more biomass. Furthermore, the addition of 30 and 300 microg l(-1) of phosphorus dramatically increased the biofilm cell metabolic potential. The addition of 3 microg l(-1) of phosphorus was found to have minor effects on the parameters examined.
  
  
  CONCLUSIONS
  The results indicate phosphorus addition to drinking water distribution system (DWDS) has a complicated effect on the biofilm formation.
  
  
  CONCLUSIONS
  As the addition of phosphorus at certain levels can affect the biofilm growth in DWDS, care should be taken when phosphate-based corrosion inhibitors are used in the DWDS.
authors.affiliation: Department of Civil Engineering, National University of Singapore, Singapore.
authors.forename: W
authors.initials: W
authors.lastname: Fang
authors.forename: J Y
authors.initials: JY
authors.lastname: Hu
authors.forename: S L
authors.initials: SL
authors.lastname: Ong
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D018613:Microscopy, Confocal; D010758:Phosphorus / Q000378:metabolism*; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*; D014881:Water Supply
chemical_list: D010758:Phosphorus