title: Sortase-mediated ligation: a gift from Gram-positive bacteria to protein engineering.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Tsukiji
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry; D010457:Peptidoglycan / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D019154:Protein Splicing; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D009696:Nucleic Acids; D010457:Peptidoglycan; D011993:Recombinant Fusion Proteins; D019881:Aminoacyltransferases; D003546:Cysteine Endopeptidases