Coumarin-derived Cu(2+)-selective fluorescence sensor: synthesis, mechanisms, and applications in living cells.
A novel coumarin-based fluorogenic probe bearing the 2-picolyl unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu(2+) over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu(2+) ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu(2+) in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu(2+).
Jung
Hyo Sung
HS
Department of Chemistry, Korea University, Seoul 136-701, Korea.
Kwon
Pil Seung
PS
Lee
Jeong Won
JW
Kim
Jae Il
JI
Hong
Chang Seop
CS
Kim
Jong Wan
JW
Yan
Shihai
S
Lee
Jin Yong
JY
Lee
Jung Hwa
JH
Joo
Taiha
T
Kim
Jong Seung
JS
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002413:Cations, Divalent; D002478:Cells, Cultured; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008253:Macaca mulatta; D008856:Microscopy, Fluorescence / Q000379:methods; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000379:methods
D002413:Cations, Divalent; D003374:Coumarins; D005456:Fluorescent Dyes; D003300:Copper