Flavonoids inhibit the AU-rich element binding of HuC.
Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC(50) values were low (0.2-1.8 microM). [BMB reports 2009; 42(1): 41-46].
Kwak
Hojoong
H
Departments of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University, College of Medicine, Seoul 110-799, Korea.
Jeong
Kyung-Chae
KC
Chae
Min-Ju
MJ
Kim
Soo-Youl
SY
Park
Woong-Yang
WY
D001483:Base Sequence; D051959:ELAV Proteins / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics*; D013698:Templates, Genetic
D051959:ELAV Proteins; D005419:Flavonoids; D038621:Regulatory Sequences, Ribonucleic Acid