Proline accumulation and transcriptional regulation of proline biosynthesis and degradation in Brassica napus.
To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding Delta(1)-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine delta-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development, proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development. [BMB reports 2009; 42(1): 28-34].
Xue
Xingning
X
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, China.
Liu
Aihua
A
Hua
Xuejun
X
D000040:Abscisic Acid / Q000494:pharmacology; D015139:Blotting, Southern; D029688:Brassica napus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics / Q000378:metabolism; D011392:Proline / Q000096:biosynthesis* / Q000378:metabolism*; D011394:Proline Oxidase / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D012965:Sodium Chloride; D000040:Abscisic Acid; D011392:Proline; D011394:Proline Oxidase; D009953:Ornithine-Oxo-Acid Transaminase