Hydrophilic fluorescent nanogel thermometer for intracellular thermometry.
The first methodology to measure intracellular temperature is described. A highly hydrophilic fluorescent nanogel thermometer developed for this purpose stays in the cytoplasm and emits stronger fluorescence at a higher temperature. Thus, intracellular temperature variations associated with biological processes can be monitored by this novel thermometer with a temperature resolution of better than 0.5 degrees C.
Gota
Chie
C
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Okabe
Kohki
K
Funatsu
Takashi
T
Harada
Yoshie
Y
Uchiyama
Seiichi
S
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D013821:Thermometers
D000180:Acrylic Resins; D005456:Fluorescent Dyes; C052970:poly-N-isopropylacrylamide