title: Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues.
abstract: OBJECTIVE
  The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues.
  
  
  METHODS
  Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation.
  
  
  RESULTS
  The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.
  
  
  CONCLUSIONS
  Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.
authors.affiliation: Department of Obstetrics and Gynecology, First People's Hospital Affiliated to Shanghai Jiao Tong University, Haining Road 100, Shanghai 200080, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Zhang
authors.forename: Lin-Xia
authors.initials: LX
authors.lastname: Li
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Liu
authors.forename: Yi-Xia
authors.initials: YX
authors.lastname: Yang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wan
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000502:physiology; D010053:Ovary / Q000637:transplantation*; D027724:Reproductive Techniques, Assisted; D013395:Sucrose
chemical_list: D003451:Cryoprotective Agents; D004958:Estradiol; D013395:Sucrose