title: Cyclometalated platinum(II) complexes as highly sensitive luminescent switch-on probes for practical application in protein staining and cell imaging.
abstract: Protein-staining platinum: The luminescent switch-on characteristic of the platinum(II) complex can be utilized for staining a series of proteins in sodium dodecyl sulfate-polyarcylamide gels, to give emissive gel images directly under UV light (see figure). The detection sensitivity for BSA protein is down to 6.0 ng, revealing potential practical applications of luminescent platinum(II) complexes in the luminescent signaling of biomolecules.
authors.affiliation: Department of Chemistry and Open Laboratory of Chemical Biology, The Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Ella Lai-Ming
authors.initials: EL
authors.lastname: Wong
authors.forename: Dik-Lung
authors.initials: DL
authors.lastname: Ma
authors.forename: Glenna So-Ming
authors.initials: GS
authors.lastname: Tong
authors.forename: Kwan-Ming
authors.initials: KM
authors.lastname: Ng
authors.forename: Chi-Ming
authors.initials: CM
authors.lastname: Che
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D047028:Macrocyclic Compounds / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure; D010984:Platinum / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D005456:Fluorescent Dyes; D047028:Macrocyclic Compounds; D015335:Molecular Probes; D011506:Proteins; D010984:Platinum