Species-specific variation of RPA-interacting protein (RIP) splice isoforms.
Replication Protein A (RPA) is a single stranded DNA-binding protein involved in DNA metabolic activities such as replication, repair, and recombination. RPA-Interacting Protein alpha (RIPalpha) was originally identified as a nuclear transporter of RPA in Xenopus. The human RIPalpha gene encodes several splice isoforms, of which hRIPalpha and hRIPbeta are the major translation products in vivo. However, limited information is available about the alternative splicing of RIPalpha in eukaryotes, apart from that in humans. In this study, we examined the alternative splicing of RIPalpha in the Drosophila, Xenopus, and mouse system. We showed that the number of splice isoforms of RIPalpha was species-specific, and displayed a tendency to increase in higher eukaryotes. Moreover, a mouse ortholog of hRIPbeta, mRIPbeta2, was not SUMOylated, in contrast to hRIPbeta. Based on these results, we suggest that the RIPalpha gene gains more splice isoforms and additional modifications after molecular evolution. [BMB reports 2009; 42(1): 22-27].
Kim
Kwangsoo
K
Korea Basic Science Institute, Gwangju Center, Gwangju 500-757, Korea.
Lee
Eun-Ju
EJ
Lee
Seung-Hoon
SH
Seo
Taegun
T
Jang
Ik-Soon
IS
Park
Junsoo
J
Lee
Je-Ho
JH
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D013045:Species Specificity; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism
D002352:Carrier Proteins; D029721:Drosophila Proteins; D026901:Membrane Transport Proteins; D020033:Protein Isoforms; C541051:RPA-interacting protein alpha, Drosophila; C541052:RPA-interacting protein alpha, mouse; D025841:Small Ubiquitin-Related Modifier Proteins; D029867:Xenopus Proteins; C121149:ripalpha protein, Xenopus