[Low paraoxonase and arylesterase plasma activities in Mexican patients with coronary artery disease].
OBJECTIVE
To determine the Paraoxonase-1 (PON1) activity as well as its pheno- and genotypes at position 192 in Mexican subjects with diagnosis of coronary heart disease (CHD).


METHODS
We determined the PON1-192 polymorphism by PCR-RFLP, and serum PON1 activity, using either paraoxon (PONase activity) or phenylacetate (ARE activity) as substrates, in 155 clinically healthy individuals (control group), and 155 patients with at least one myocardial infarction (CHD group). The biochemical A/B phenotype was determined by the ratio of the NaCI 1 M-stimulated PONase activity divided by the ARE activity.


RESULTS
We found significantly lower PONase and ARE activities in CHD patients as compared to controls (233.1 +/- 102.1 vs. 295.8 +/- 159.1 nmol/min/mL, and 103.1 +/- 33.7 vs 220.2 +/- 120.7 micromol/min/mL, respectively, p<0.05 for both). Allele and genotype frequencies for PON1-192 were similar in CHD patients and healthy controls. Moreover, in the control group, the PON1-192 Q/R genotype did not matched with the A/B phenotype as has been proposed by other studies.


CONCLUSIONS
There were important differences in the ARE and PONase activities between Mexican CHD patients and controls, suggesting that PON1 activity could be a good marker of CHD risk, whereas PON1-192 lacks of value to assess such risk.
Gamboa
Ricardo
R
Departamento de Fisiología, Instituto Nacional de Cardiología Ignacio Chávez, DF México.
Regalado
Juan Carlos
JC
Huesca-Gómez
Claudia
C
Posadas-Romero
Carlos
C
Verdejo Paris
Juan
J
Vargas-Alarcón
Gilberto
G
Pérez-Méndez
Oscar
O
D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000235:genetics; D008076:Cholesterol, HDL / Q000097:blood; D003324:Coronary Artery Disease / Q000201:enzymology*; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology; D010641:Phenotype; D011110:Polymorphism, Genetic
D008076:Cholesterol, HDL; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase