title: Thermodynamics of partitioning of substance P in isotropic bicelles.
abstract: The temperature dependence of the partition of a neuropeptide, substance P (SP), in isotropic (q = 0.5) bicelles was investigated by using pulsed field gradient NMR diffusion technique. The partition coefficient decreases as the temperature is increased from 295 to 325 K, indicating a favorable (negative) enthalpy change upon partitioning of the peptide. Thermodynamic analysis of the data shows that the partitioning of SP at 300 K is driven by the enthalpic term (DeltaH) with the value of - 4.03 kcal mol(-1), while it is opposed by the entropic term (-TDeltaS) by approximately 1.28 kcal mol(-1) with a small negative change in heat capacity (DeltaC(p)). The enthalpy-driven process for the partition of SP in bicelles is the same as in dodecylphosphocholine (DPC) micelles, however, the negative entropy change in bicelles of flat bilayer surface is in sharp contrast with the positive entropy change in DPC micelles of highly curved surface, indicating that the curvature of the membrane surface might play a significant role in the partitioning of peptides.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Kim
authors.forename: Seung Bin
authors.initials: SB
authors.lastname: Baek
authors.forename: Do Hun
authors.initials: DH
authors.lastname: Kim
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Lim
authors.forename: Hyeong Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hee Cheon
authors.initials: HC
authors.lastname: Lee
mesh_terms: D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013373:Substance P / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D008051:Lipid Bilayers; D013373:Substance P