Immunodiagnosis of human neurocysticercosis using a synthetic peptide selected by phage-display.
The usefulness of a synthetic peptide in the serodiagnosis of Taenia solium human neurocysticercosis (NC) has been evaluated. Phage-displayed peptides were screened with human antibodies to scolex protein antigen from cysticercus cellulosae (SPACc). One clone was found to interact specifically with anti-SPACc IgGs. The corresponding synthetic peptide was found to be recognized in ELISA by NC patient's sera. The study was carried out with sera from 28 confirmed NC patients, 13 control sera and 73 sera from patients suffering from other infectious diseases. A 93% sensibility and a 94.3% specificity was achieved. Figures of 89% and 31.4% of sensibility and specificity were obtained in a SPACc-based ELISA. Immunoblotting of SPACc with anti-peptide antibodies revealed a single band of approximately 45 kDa in 1D and four 45 kDa isoforms in 2D-gel electrophoresis. A strong and specific immunostaining in the fibers beneath the suckers, at the base of the rostellum, and in the tissue surrounding the scolex of cysticerci was observed by immunomicroscopy. Our results show that a peptide-based immunodiagnostic of neurocisticercosis can be envisioned.
Hell
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RC
Departamento de Bioquímica-Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
Amim
P
P
de Andrade
H M
HM
de Avila
R A M
RA
Felicori
L
L
Oliveira
A G
AG
Oliveira
C A
CA
Nascimento
E
E
Tavares
C A P
CA
Granier
C
C
Chávez-Olórtegui
C
C
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D007159:Immunologic Tests / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D020019:Neurocysticercosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D016133:Polymerase Chain Reaction; D041201:Taenia solium / Q000276:immunology* / Q000302:isolation & purification
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth; D019151:Peptide Library; D010455:Peptides