Evaluation of urothelial stretch-induced cyclooxygenase-2 expression in a mouse ureteral obstruction model.
Prostanoids play a major role in the nociceptive response to ureteral obstruction. Cyclooxygenases (COXs) 1 and 2 catalyze the rate-limiting step in prostanoid synthesis; COX-2 is the more inducible isoform. Previous studies in human and animal models have shown that COX-2 is highly induced during ureteral obstruction. Our objective was to characterize acute COX-2 induction in a reproducible mouse model. Unilateral ureteral ligation was performed, and obstruction was maintained for 2, 4, 6, 8, or 12 hours. We evaluated COX-2 protein expression using Western immunoblotting, and found that ureteral obstruction induced COX-2 expression ninefold within 6 hours. This is the first report to characterize in vivo temporal stretch-induced COX-2 expression in a mouse model. This model will be critical for elucidation of COX-2 signaling pathways and may eventually help to identify novel therapeutic targets for treating ureteral obstruction.
Sterrett
Samuel P
SP
Department of Urology, University of Wisconsin, Madison, Wisconsin, USA.
Wilkinson
Eric R
ER
Jerde
Travis J
TJ
Mellon
William S
WS
Nakada
Stephen Y
SY
D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D015151:Immunoblotting; D051379:Mice; D013314:Stress, Mechanical; D013537:Sutures; D013997:Time Factors; D014517:Ureteral Obstruction / Q000201:enzymology*; D019459:Urothelium / Q000201:enzymology*
C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2