title: Disruption of Vps4 and JNK function in Drosophila causes tumour growth.
abstract: Several regulators of endocytic trafficking have recently been identified as tumour suppressors in Drosophila. These include components of the endosomal sorting complex required for transport (ESCRT) machinery. Disruption of subunits of ESCRT-I and -II leads to cell-autonomous endosomal accumulation of ubiquitinated receptors, loss of apicobasal polarity and epithelial integrity, and increased cell death. Here we report that disruption of the ATPase dVps4, the most downstream component of the ESCRT machinery, causes the same array of cellular phenotypes. We find that loss of epithelial integrity and increased apoptosis, but not loss of cell polarity, require the activation of JNK signalling. Abrogation of JNK signalling prevents apoptosis in dVps4 deficient cells. Indeed double deficiency in dVps4 and JNK signalling leads to the formation of neoplastic tumours. We conclude that dvps4 is a tumour suppressor in Drosophila and that JNK is central to the cell-autonomous phenotypes of ESCRT-deficient cells.
authors.affiliation: Faculty of Medicine, Centre for Cancer Biomedicine, University of Oslo and Institute for Cancer Research, the Norwegian Radium Hospital, Rikshospitalet University Hospital, Montebello, Oslo, Norway.
authors.forename: Lina M
authors.initials: LM
authors.lastname: Rodahl
authors.forename: Kaisa
authors.initials: K
authors.lastname: Haglund
authors.forename: Catherine
authors.initials: C
authors.lastname: Sem-Jacobsen
authors.forename: Franz
authors.initials: F
authors.lastname: Wendler
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Vincent
authors.forename: Karine
authors.initials: K
authors.lastname: Lindmo
authors.forename: Tor Erik
authors.initials: TE
authors.lastname: Rusten
authors.forename: Harald
authors.initials: H
authors.lastname: Stenmark
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D016764:Cell Polarity; D049109:Cell Proliferation; D002470:Cell Survival; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D056827:Endosomal Sorting Complexes Required for Transport; D004848:Epithelium / Q000473:pathology; D017353:Gene Deletion; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000166:cytology / Q000648:ultrastructure; D020935:MAP Kinase Signaling System; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000201:enzymology* / Q000473:pathology*; D017386:Sequence Homology, Amino Acid; D054875:Ubiquitination; D015854:Up-Regulation / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D016023:Integrins; D048031:JNK Mitogen-Activated Protein Kinases; D020781:Matrix Metalloproteinase 1; D000251:Adenosine Triphosphatases; C539468:Vps4 protein, Drosophila