Novel method for determination of substance P levels in unextracted human plasma by using acidification.
Substance P (SP) is a member of the tachykinin family and has an important role in immune responses. SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction.
Campbell
Donald E
DE
Joseph Stokes Jr. Research Institute at the Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA. campbelld@email.chop.edu
Bruckner
Paula
P
Tustin
Nancy B
NB
Tustin
Richard
R
Douglas
Steven D
SD
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma / Q000737:chemistry*; D013373:Substance P / Q000032:analysis*
D013373:Substance P