title: Viral agents associated with acute gastroenteritis in children hospitalized with diarrhea in Lanzhou, China.
abstract: BACKGROUND
  Gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Rotavirus, human caliciviruses (HucV), adenovirus, and astrovirus are recognized as common etiologies of acute gastroenteritis.
  
  
  OBJECTIVE
  To use antigen detection and molecular methods to determine the viral etiology of childhood diarrhea in Lanzhou, China, 2005-2007.
  
  
  METHODS
  544 stool specimens were collected from children hospitalized with diarrhea. ELISA, RT-PCR, or PCR were used to detect viruses commonly causing diarrhea.
  
  
  RESULTS
  Group A rotavirus, norovirus, sapovirus, astrovirus, and adenovirus, were detected in 54.0%, 9.2%, 1.1%, 3.3%, and 4.4%, respectively. No group B or group C rotaviruses were detected. The relative contribution of these viruses changed greatly over 2 years. The percentage of rotavirus and adenovirus dropped from 61.2% and 5.4% to 47.6% and 1.4%, whereas HucV increased from 5.0% to 15.0%. G1 and P[8] were the predominant rotavirus strains, and P[6] was detected for the first time in this area. The predominant norovirus strain changed from GII3 to GII4, and the subtypes of GII4 changed from the Hunter strain to the variant 2006b strain.
  
  
  CONCLUSIONS
  The distribution of viruses and genotypes of individual viruses causing gastroenteritis in Lanzhou, China changed greatly during 2005-2007.
authors.affiliation: Department of Pediatrics, The First Hospital of Lanzhou University, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Wei-xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Yang
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zi-qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Jie-mei
authors.initials: JM
authors.lastname: Yu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Su-hua
authors.initials: SH
authors.lastname: Yang
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Cui
authors.forename: Zhao-yin
authors.initials: ZY
authors.lastname: Fang
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D002675:Child, Preschool; D002681:China; D003967:Diarrhea / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014777:Virus Diseases / Q000821:virology*; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification*