Interaction of tea catechin (-)-epigallocatechin gallate with lipid bilayers.
A major component of green tea extracts, catechin (-)-Epigallocatechin gallate (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 microM caused GUVs to burst. Here we investigated the effect of EGCg on single GUVs at lower concentrations, believing that its molecular mechanism would be more clearly revealed. We used the micropipette aspiration method, by which the changes of surface area and volume of a GUV could be measured as a result of interaction with EGCg. We also used x-ray diffraction to measure the membrane thinning effect by EGCg. To understand the property of EGCg, we compared its effect with other membrane-active molecules, including pore-forming peptide magainin, the turmeric (curry) extract curcumin, and detergent Triton X100. We found the effect of EGCg somewhat unique. Although EGCg readily binds to lipid bilayers, its membrane area expansion effect is one order of magnitude smaller than curcumin. EGCg also solubilizes lipid molecules from lipid bilayers without forming pores, but its effect is different from that of Triton X100.
Sun
Yen
Y
Department of Physics and Astronomy, Rice University, Houston, Texas 77251, USA.
Hung
Wei-Chin
WC
Chen
Fang-Yu
FY
Lee
Chang-Chun
CC
Huang
Huey W
HW
D000818:Animals; D002151:Calorimetry; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D003474:Curcumin / Q000378:metabolism / Q000494:pharmacology; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D054806:Magainins / Q000378:metabolism / Q000494:pharmacology; D017830:Octoxynol / Q000378:metabolism / Q000494:pharmacology; D016062:Porosity; D012995:Solubility; D013662:Tea / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D054806:Magainins; D013662:Tea; D053835:Unilamellar Liposomes; D002392:Catechin; D017830:Octoxynol; C045651:epigallocatechin gallate; D003474:Curcumin