title: Virological and antigenic characterization of two Peste des Petits Ruminants (PPR) vaccine viruses of Indian origin.
abstract: Virological and antigenic characteristics of two Indian Peste des Petits Ruminants (PPR) vaccine viruses namely "PPRV-Sungri/96" and "PPRV-AR/87" were investigated. This investigation included, type of cytopathic effect (CPE) produced, one-step growth curve, antigenic reactivity of viral antigens using a panel of 18 monoclonal antibodies (MAbs) and ability of viruses for neutralization using a monoclonal antibody directed to haemagglutinin (H) protein. Findings surprisingly indicated that the PPRV-AR/87 is a fast growing virus with an entirely different pattern of cytopathic effect in Vero cell system. This virus has a relatively short replication cycle of 21h with no clearly defined eclipse phase. Whereas, PPRV-Sungri/96 has a replication cycle of 72h with distinct eclipse phase. Both viruses showed very good antigenic correlation (r=0.823) based on reactivity with 18 MAbs in an indirect ELISA, indicating that they are closely antigenically related. PPRV-AR/87 showed a poor neutralization index with antihaemaglutinin MAb 4B11 and poor reactivity in sandwich ELISA using an anti-nucleocapsid (N) MAb as compared to PPRV-Sungri/96. The findings suggest that both these vaccine viruses can easily be differentiated based on the pattern of cytopathic effect and degree of neutralization using MAb 4B11.
authors.affiliation: Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, Uttar Pradesh, India. rpsingh@ivri.up.nic.in
authors.forename: R P
authors.initials: RP
authors.lastname: Singh
authors.forename: U K
authors.initials: UK
authors.lastname: De
authors.forename: K D
authors.initials: KD
authors.lastname: Pandey
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D007194:India / Q000453:epidemiology; D029021:Peste-des-Petits-Ruminants / Q000453:epidemiology / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000145:classification* / Q000502:physiology*; D013997:Time Factors; D014709:Vero Cells; D014765:Viral Vaccines
chemical_list: D000956:Antigens, Viral; D014765:Viral Vaccines