title: Determination of norfloxacin and enrofloxacin by solid-phase microextraction/high-performance liquid chromatography.
abstract: A solid-phase microextraction (SPME) method followed by separation with high-performance liquid chromatography and subsequent UV detection was developed for the determination of norfloxacin and enrofloxacin. The simple and sensitive preconcentration technique uses 280 nm wavelength in mobile phase of citrate buffer (0.01 M), pH 3.8, prepared in water (A) and acetonitrile (B), with composition of the mobile phase A:B, 40:60, at a flow rate of 1.0 mL/min. A C18 reversed-phase analytical column (5 microm) was selected as separation medium for the technique. To obtain optimum extraction efficiency, several parameters relating to SPME were investigated. The method was linear over the range of 10-100 ng/mL for norfloxacin and enrofloxacin with a correlation coefficient (R2) value of 0.9972 and 0.9980 for norfloxacin and enrofloxacin, respectively. Using the SPME method, the detection limits (signal-to-noise ratio = 3) are 0.17 and 0.12 ng/mL for norfloxacin and enrofloxacin, respectively.
authors.affiliation: Punjabi University, Department of Chemistry, Patiala-147002, India.
authors.forename: Ashwini
authors.initials: A
authors.lastname: Kumar
authors.forename: Gaurav
authors.initials: G
authors.lastname: Dhingra
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Malik
authors.forename: Dhananjay K
authors.initials: DK
authors.lastname: Tewary
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009643:Norfloxacin / Q000032:analysis* / Q000652:urine; D052617:Solid Phase Microextraction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D009643:Norfloxacin