Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors.
Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M(1-4) muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
Pradidarcheep
Wisuit
W
Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Stallen
Jan
J
Labruyère
Wil T
WT
Dabhoiwala
Noshir F
NF
Michel
Martin C
MC
Lamers
Wouter H
WH
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D015153:Blotting, Western; D001923:Brain Chemistry; D002460:Cell Line; D041981:Gastrointestinal Tract / Q000737:chemistry; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007150:Immunohistochemistry; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D011976:Receptors, Muscarinic / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D014551:Urinary Tract / Q000737:chemistry
D000906:Antibodies; D007106:Immune Sera; D011941:Receptors, Adrenergic; D011976:Receptors, Muscarinic