Generation of Vibrio anguillarum ghost by coexpression of PhiX 174 lysis E gene and staphylococcal nuclease A gene.
Vibrio anguillarum ghosts (VAG) were generated, for the first time, using a conjugation vector containing a ghost bacteria inducing cassette, pRK-lambdaP(R)-cI-Elysis, in which the expression of PhiX174 lysis gene E was controlled by the P ( R )/cI regulatory system of lambda phage. By scanning electron microscopy, holes ranging 80-200 nm in diameter were observed in the VAG. To avoid the presence of bacterial genomic DNA and an antibiotic resistance gene in the final VAG product, we constructed a new dual vector, pRK-lambdaP(R)-cI-E-SNA, containing the E-mediated lysis cassette and the staphylococcal nuclease A (SNA)-mediated DNA degradation cassette, and generated safety-enhanced VAG for use as a fish vaccine.
Kwon
Se Ryun
SR
Department of Aquatic Life Medicine, Pukyong National University, Pusan, Korea.
Kang
Yue Jai
YJ
Lee
Dong Jin
DJ
Lee
Eun Hye
EH
Nam
Yoon Kwon
YK
Kim
Sung Koo
SK
Kim
Ki Hong
KH
D001428:Bacterial Vaccines / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008836:Micrococcal Nuclease / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000166:cytology* / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D001428:Bacterial Vaccines; D008836:Micrococcal Nuclease; D014764:Viral Proteins; C052951:3 protein, Bacillus phage Nf