title: Identification of a novel common proviral integration site, flit-1, in feline leukemia virus induced thymic lymphoma.
abstract: A new proviral integration site for feline leukemia virus (FeLV), termed flit-1, was identified from feline thymic lymphoma. Among 35 FeLV-related tumors examined, 5 of 25 thymic lymphomas demonstrated proviral insertion within flit-1 locus whereas none of four alimentary and five multicentric lymphomas and one T-lymphoid leukemia examined had rearrangement in this region. Extensive sequence analysis has shown that flit-1, which is noncoding, is conserved on human chromosome 12 and mouse chromosome 15. The human and murine homologs of flit-1 are positioned approximately 30-kb upstream to activin-A receptor type II-like 1 (ACVRL1/ALK1) gene. Expression of ACVRL1 mRNA was examined in two of five lymphomas with flit-1 rearrangement and detected in both of the two whereas normal thymuses and seven lymphoid tumors without flit-1 rearrangement had no detectable expression. Therefore, flit-1 appears to represent a novel FeLV proviral common integration domain that may influence lymphomagenesis as insertional mutagenesis.
authors.affiliation: Department of Pathology, University of Southern California School of Medicine, Los Angeles, CA 90033, USA. afujino@mail.ecc.u-tokyo.ac.jp
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Chun-Peng
authors.initials: CP
authors.lastname: Liao
authors.forename: Yan Shi
authors.initials: YS
authors.lastname: Zhao
authors.forename: Judong
authors.initials: J
authors.lastname: Pan
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Mathes
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Hayes
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Pradip
authors.initials: P
authors.lastname: Roy-Burman
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D023061:Gene Order; D015321:Gene Rearrangement; D005256:Leukemia Virus, Feline / Q000502:physiology*; D008223:Lymphoma / Q000473:pathology / Q000821:virology*; D011533:Proviruses / Q000502:physiology*; D013950:Thymus Gland / Q000473:pathology / Q000821:virology*; D016662:Virus Integration