Myelin basic protein-reactive T cells persist in an inactive state in the bone marrow of Lewis rats that have recovered from autoimmune encephalomyelitis.
Lewis rats immunized with guinea pig myelin basic protein residues 68-86 develop acute experimental autoimmune encephalomyelitis and recover. The predominant T cell receptor expressed by the encephalitogenic T cells is TCRBV8S2. They persist in bone marrow many weeks after recovery. CD3 is down-regulated, but >90% express CD4. They fail to proliferate to GPMBP68-86 unless a nitric oxide synthase inhibitor is added to the cultures. Perhaps these are memory T cells that are maintained in a suppressed state in BM by a nitric oxide-dependent mechanism.
Kheradmand
Taba
T
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Wolf
Norbert A
NA
Swanborg
Robert H
RH
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D004676:Myelin Basic Protein / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010446:Peptide Fragments / Q000633:toxicity; D051381:Rats; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism; D020127:Recovery of Function / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
D017252:CD3 Complex; D015704:CD4 Antigens; D004791:Enzyme Inhibitors; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase