Protein self-interaction chromatography on a microchip.
This paper presents the development of a novel miniaturized experimental procedure for the measurement of protein-protein interactions through Self-Interaction Chromatography (SIC) on a microchip, without the use of chromatographic resins. SIC was recently demonstrated to be a relatively easy method to obtain quantitative thermodynamic information about protein-protein interactions, like the osmotic second virial coefficient B(22), which relates to protein phase behavior including protein crystallization. This successful miniaturization to microchip level of a measurement device for protein self-interaction data is a first key step to a complete microfluidic screening platform for the rational design of protein crystallizations, using substantially less expensive protein and experimentation time.
Deshpande
Kedar
K
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628BC, Delft, The Netherlands.
Ahamed
Tangir
T
van der Wielen
Luuk A M
LA
Horst
Joop H Ter
JH
Jansens
Peter J
PJ
Ottens
Marcel
M
D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D005456:Fluorescent Dyes / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D025941:Protein Interaction Mapping; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D012965:Sodium Chloride; D009113:Muramidase