title: Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells.
abstract: OBJECTIVE
  Isolating spermatogonia cells with high purity and viability and achieving better survival rate following cryopreservation
  
  
  METHODS
  Isolating the cells by Magnetic Activating Cell Sorting (MACS) method using anti CD49f (alpha6 integrin) antibody and Dynabeads and freezing in DMSO-based freezing mediums containing three different FBS concentrations of 50%, 60% and 70%.
  
  
  RESULTS
  The mean (+/-SD) purity of the isolated cells was 92.52+/-3.57 (range 92.43-98.25). The cells frozen in group I, II and III had mean 39.60+/-1.48 (range 37.98-41.62), 89.05+/-3.83 (range 80.83-90.33) and 90.52+/-1.71 (range 89.07-92.52) viability, respectively.
  
  
  CONCLUSIONS
  Higher viable cell counts and purity can be attained by the use of alpha6 integrin and magnetic beads. After the thawing of spermatogonial cells, optimum viability was achieved in freezing media containing 60% FBS.
authors.affiliation: Royan Institute,Andrology Lab, P.O.Box: 19395-4644, Tehran, Iran. firoozjannat@gmail.com
authors.forename: Firooz Jannat
authors.initials: FJ
authors.lastname: Alipoor
authors.forename: Mohammad Ali Sadighi
authors.initials: MA
authors.lastname: Gilani
authors.forename: Poopak
authors.initials: P
authors.lastname: Eftekhari-Yazdi
authors.forename: Ali Daliri
authors.initials: AD
authors.lastname: Hampa
authors.forename: Hani
authors.initials: H
authors.lastname: Hosseinifar
authors.forename: Hiva
authors.initials: H
authors.lastname: Alipour
authors.forename: Mehdi Lotfi
authors.initials: ML
authors.lastname: Panah
mesh_terms: D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation; D005434:Flow Cytometry; D018189:Immunomagnetic Separation; D008297:Male; D051379:Mice; D027724:Reproductive Techniques, Assisted; D012741:Sexual Maturation; D013093:Spermatogonia / Q000502:physiology / Q000637:transplantation