A possible role for telomerase RNA and telomere length in global mitotic recombination.
Telomeres are specialised structures at the ends of mammalian chromosomes with many unique properties. Recombinational events at telomeres are more frequent than in the remainder of the genome by several orders of magnitude. This study examined the influence of telomerase status and telomere length on genome-wide recombination assessed by genomic sister chromatid exchange (G-SCE). Telomerase deficiency per se appears to increase G-SCE frequencies in splenocytes but as telomeres shorten through subsequent generations of mTerc(-/-) mice this increase is progressively lost. Telomerase status and telomere length also influences the induction of G-SCE by UV light. Even when mitotic recombination is affected by PARP deficiency, mTerc and telomere length interact to further affect G-SCE frequencies. Taken together the data presented here demonstrate that telomerase status and telomere length can affect recombination frequencies genome-wide.
Bouffler
S D
SD
Health Protection Agency, Radiation Protection Division, Chilton, Didcot, UK. simon.bouffler@hpa.org.uk
Finnon
P
P
Blasco
M A
MA
Ainsbury
E
E
D000704:Analysis of Variance; D000818:Animals; D002453:Cell Cycle; D015971:Gene Expression Regulation, Enzymologic; D005787:Gene Frequency; D005838:Genotype; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008938:Mitosis / Q000235:genetics*; D011065:Poly(ADP-ribose) Polymerases / Q000172:deficiency / Q000235:genetics; D012313:RNA / Q000235:genetics*; D011995:Recombination, Genetic; D012854:Sister Chromatid Exchange / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000201:enzymology; D019098:Telomerase / Q000172:deficiency / Q000235:genetics*; D016615:Telomere / Q000235:genetics* / Q000648:ultrastructure
C422952:telomerase RNA; D012313:RNA; D011065:Poly(ADP-ribose) Polymerases; D019098:Telomerase