A double-injection model of intracerebral hemorrhage in rabbits.
We aimed to develop a double-injection model of intracerebral hemorrhage (ICH) in rabbits and to evaluate it as a tool for investigating post-ICH brain injury. Rabbits were injected with 300microL fresh autologous whole blood into the right basal ganglia. Behavioral changes were rated, brain water content (BWC) was measured and brain tissue morphology was also examined. ICH was established in 93.5% of the blood injection group. At 1, 3 and 7 days after ICH, there were significant differences in the total neurological scores (p<0.01) and BWC (p<0.01) between a sham-operated group and the ICH group. These findings suggest that the model produces a persistent neurological deficit, hematoma volume and perihematomal edema and closely mimics human hypertensive basal ganglia ICH; it is a controllable and reproducible hematoma that lends itself to quantitative investigation.
Yu
Zhen
Z
Department of Neurology, The Second Affiliated Hospital, Chongqing University of Medical Sciences, 76 Lin Jiang Men, Chongqing 400010, China.
Chen
Li-Fen
LF
Li
Xiao-Feng
XF
Zhang
Dong-Ping
DP
Chen
Yang-Mei
YM
Wu
Wan-Fu
WF
Hu
Chang-Lin
CL
D000818:Animals; D020145:Basal Ganglia Hemorrhage / Q000473:pathology / Q000503:physiopathology; D001522:Behavior, Animal; D001777:Blood Coagulation / Q000502:physiology; D001929:Brain Edema / Q000209:etiology; D002543:Cerebral Hemorrhage / Q000150:complications / Q000209:etiology / Q000473:pathology; D003243:Consciousness / Q000502:physiology; D004195:Disease Models, Animal; D007267:Injections; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009422:Nervous System Diseases / Q000209:etiology; D011817:Rabbits; D013997:Time Factors