title: Particle-based synthesis of peptide arrays.
abstract: Lithographic methods allow for the combinatorial synthesis of >50,000 oligonucleotides per cm(2), and this has revolutionized the field of genomics. High-density peptide arrays promise to advance the field of proteomics in a similar way, but currently lag behind. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with lithography, which adds up to an excessive number of coupling cycles. Combinatorial synthesis based on electrically charged solid amino acid particles resolves this problem. A color laser printer or a chip addresses the different charged particles consecutively to a solid support, where, when completed, the whole layer of solid amino acid particles is melted at once. This frees hitherto immobilized amino acids to couple all 20 different amino acids to the support in one single coupling reaction. The method should allow for the translation of entire genomes into sets of overlapping peptides to be used in proteome research.
authors.affiliation: Department of Chip-Based Peptide Arrays, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. f.breitling@dkfz.de
authors.forename: Frank
authors.initials: F
authors.lastname: Breitling
authors.forename: Thomas
authors.initials: T
authors.lastname: Felgenhauer
authors.forename: Alexander
authors.initials: A
authors.lastname: Nesterov
authors.forename: Volker
authors.initials: V
authors.lastname: Lindenstruth
authors.forename: Volker
authors.initials: V
authors.lastname: Stadler
authors.forename: F Ralf
authors.initials: FR
authors.lastname: Bischoff
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D003201:Computers; D007834:Lasers; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000596:Amino Acids; D010455:Peptides