title: [Ectopic expression of BCSC-1 gene results in enhancement of adhesion and cell cycling blockade of nasopharyngeal carcinoma CNE-2L2 cell].
abstract: OBJECTIVE
  To study mechanisms of reduction of the malignant activities of human naso-pharyngeal carcinoma cell CNE-2L2 induced by ectopic expression of BCSC-1 gene.
  
  
  METHODS
  DNA was stained with propidium iodide and assayed upon a flow cytometer. Chromosomes were stained with Hoechest 33258. Adhesion of CNE-2L2 cells was detected by cell aggregation test. Protein expression on CNE-2L2 cells was examined by Western blot.
  
  
  RESULTS
  Cell cycle analysis showed that the percentage of CNE-2L2 cells was 55.1%, 43.4%, and 39.4% in G0/G1 phase, 25.2%, 28.7%, and 30.9% in S phase, and 19.7%, 27.9%, and 29.7% in G2/M phase for the cell with ectopic expression of BCSC-1 gene, wild type cell (W cells), and the cell transduced with the mock (M cell). Many mitotic cells were found in W cells and M cells. In contrast, almost no mitotic cell was observed in the cells with ectopic expression of BCSC-1 gene. Ectopic BCSC-1 expression resulted in cell aggregation, enhanced expression of E-cadherin, cx-catenin, and p53.
  
  
  CONCLUSIONS
  Ectopic BCSC-1 expression causes enhancement of adhesion of CNE-2L2 cells associated with enhanced expression of E-cadherin and alpha-catenin, arrest of cell in G1 phase, which may be associated with enhanced expression of p53. These alteration may play a role in the reduction of malignant activities of the cells with ectopic expression of BCSC-1 gene.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Shuang-ling
authors.initials: SL
authors.lastname: Chen
authors.forename: Yi-qun
authors.initials: YQ
authors.lastname: Zhou
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.forename: Ji-yu
authors.initials: JY
authors.lastname: Ju
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhu
mesh_terms: D002448:Cell Adhesion; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D009303:Nasopharyngeal Neoplasms; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D009363:Neoplasm Proteins; C109969:VWA5A protein, human