Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP).
Hepcidin is known to be expressed in fish with bacterial challenge and iron overload. Here we first report the hepcidin expression induced in the developmental stages from embryo to fry of red sea bream (Pagarus major) and in juvenile black porgy (Acanthopagrus schlegelii B.) upon continuous waterborne exposure to BaP. The gene expression of CYP1A1 and IgL (immunoglobulin light chain) were both measured. Expression of the Pagarus major hepcidin gene (PM-hepc) was increased in post hatch fry at 24 h and 120 h exposure to BaP at concentrations of 0.1, 0.5 and 1.0 microg/l, respectively. The gene expression pattern was comparable to that of CYP1A1 but different from that of IgL. In addition, a high number of AS-hepc2 transcripts (Acanthopagrus schlegelii B. hepcidin gene) were detected in the liver upon exposure to 1.0 microg/l BaP. This study demonstrates that hepcidin gene expression is significantly induced in BaP-exposed red sea bream and black porgy.
Wang
Ke-Jian
KJ
State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian 361005, PR China. wkjian@xmu.edu.cn
Bo
Jun
J
Yang
Ming
M
Hong
Hua-Sheng
HS
Wang
Xin-Hong
XH
Chen
Fang-Yi
FY
Yuan
Jian-Jun
JJ
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004781:Environmental Exposure; D005786:Gene Expression Regulation / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D064451:Hepcidins; D010473:Perciformes / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream / Q000502:physiology*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D023181:Antimicrobial Cationic Peptides; D064451:Hepcidins; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1