[Effects of human alpha-mannosidase Man2c1 transgene on growth and metastasis of transplanted tumor in mice].
OBJECTIVE
To study the effect of human alpha-mannosidase Man2c1 transgene on tumor growth and metastasis in mice.


METHODS
Hepatoma cell H22 or squamous epithelial carcinoma cell S180 was subcutaneously inoculated into the right armpit of mice (wild type mice and 28#, 35#, and 54# transgenic mice). Tumor size was measured every week. Mice were sacrificed on day 9 or 10 and then the tumors were exercised and weighted. Tumors and lungs were fixed in formaldehyde and sectioned. The sections were stained with hematoxylin/eosin and examined under microscope. The red blood cells in spleen were destroyed by Tris-NH4Cl. Natural killer (NK) cell activity was detected with Yac-1 cell as target.


RESULTS
H22 and S180 tumors grew faster in all the three transgenic mice (28#, 35#, and 54#) than in wild type mice. The average size and weight of tumors between the transgenic mice and wild type mice were significantly different (P<0.05). Most tumors in the transgenic mice invaded the surrounding tissues. In contrast, nearly all the tumors in wild type mice were capsulized. Three of 10 28# transgenic mice, 5 of 10 35# transgenic mice, 3 of 10 54# transgenic mice, and 1 of 10 wild type mice showed lung metastasis of H22 tumor. Two of 6 28# transgenic mice, 3 of 6 35# transgenic mice, 1 of 6 54# transgenic mice, and 0 of 6 wild type mice showed lung metastasis of S180 tumor. No difference of NK activity in spleen cells was observed between the transgenic mice and wild type mice.


CONCLUSIONS
hMan2c1 transgene promotes growth, invasion, and metastasis of transplanted H22 and S180 tumors in mice. hMan2cl transgene does not affect NK activity in splenocytes.
Jiang
Dong-dong
DD
Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Liu
Yu-qin
YQ
Gu
Bei
B
Xiang
Zhi-guang
ZG
Tian
Yun
Y
Zhou
Yi-qun
YQ
Ju
Ji-yu
JY
Liu
Yin
Y
Zhang
Lian-feng
LF
Zhu
Li-ping
LP
D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000556:secondary*; D008361:Mannosidases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D013154:Spleen / Q000276:immunology; D019076:Transgenes; D043323:alpha-Mannosidase
D008361:Mannosidases; C511245:MAN2C1 protein, human; D043323:alpha-Mannosidase