title: Offline and online liquid chromatography mass spectrometry in quantitative proteomics.
abstract: Hyphenation with liquid chromatography has become indispensable in mass spectrometry-based proteomics. Sample complexity together with the large variations in dynamic range can be only tackled using techniques that isolate and/or concentrate individual components prior to mass spectrometric analysis. In this review the most recent developments in micro/nanoliquid chromatography interfaced with MALDI and electrospray ionisation are discussed. Particular attention is focused on all applications related to quantitative proteomics.
authors.affiliation: Shimadzu Research Laboratory (Europe), Trafford Wharf Road, Wharf Side, Manchester M171GP, UK.
authors.forename: Jessica Z
authors.initials: JZ
authors.lastname: Bereszczak
authors.forename: Francesco L
authors.initials: FL
authors.lastname: Brancia
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009862:Online Systems; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D011506:Proteins