Comparative proteomic analysis of osteosarcoma cell and human primary cultured osteoblastic cell.
To identify new biomarkers that facilitate the accurate early diagnosis of osteosarcoma and that may possibly include novel therapeutic candidates, we performed a proteomic approach to compare osteosarcoma cells and human primary cultured osteoblastic cells. Image analysis of silver-stained 2-DE gels revealed that the level of 12 protein spots was significantly different between the two groups of samples (p < .004). After mass spectroscopic identification and database searches, we found that in osteosarcoma cells, the level of HSP70, actin capping protein, ATP synthase, Mthsp75, UQCRC1, Ras-related nuclear protein, UCH-L1, and PRDX4 was elevated. However, the level of pyruvate dehydrogenase E1, Prohibitin, and Annexin V was decreased. Subsequent Western blot analyses of UQCRC1, UCH-L1, and PRDX4 in osteosarcoma tissues confirmed the results obtained by the proteomic analyses. These identified proteins may be potential molecular targets for osteosarcomatous diagnostics and therapeutics.
Liu
Xudong
X
Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai JiaoTong University, Shanghai, China. Doctorliu@yahoo.cn
Zeng
Bingfang
B
Ma
Jie
J
Wan
Chunling
C
D015153:Blotting, Western; D001859:Bone Neoplasms / Q000737:chemistry*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D010006:Osteoblasts / Q000737:chemistry*; D012516:Osteosarcoma / Q000737:chemistry*; D054464:Peroxiredoxins / Q000032:analysis; D020543:Proteome; D006180:Proton-Translocating ATPases / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D043222:Ubiquitin Thiolesterase / Q000032:analysis
D018840:HSP70 Heat-Shock Proteins; D009363:Neoplasm Proteins; D020543:Proteome; C482860:UCHL1 protein, human; C515980:PRDX4 protein, human; D054464:Peroxiredoxins; D043222:Ubiquitin Thiolesterase; D006180:Proton-Translocating ATPases