title: Synthesis and cell activity of novel galactosylated chitosan as a gene carrier.
abstract: It is important for gene carrier to transport DNA into target cells. Although viral vectors are very efficient gene-transfer vehicles, significant drawbacks limit their applications. Chitosan (CS) has been researched widely as a non-viral vector. However, the low cell specificity and low transfection efficiency of chitosan need to be overcome. In order to conquer the drawback of chitosan, the present paper is concerned with the synthesis of novel galactosylated chitosan (GC) through etherization of chitosan and galactose in THF using BF(3).OEt(2) as promoter. The final product was characterized and confirmed by FT-IR and (1)H NMR. The degree of O-substitution (DS) of chitosan by galactose was measured to be 10.38% using anthrone-sulfuric acid colorimetric method. The mean particle diameter and average zeta potential of the GC/DNA complex were 350 nm and +22.1 mV, respectively. The GC/DNA nanoparticle was tested to transfect HEK293 cells, and the viability of HEK293 cells was not affected by the GC/DNA nanoparticle compared to that of the control.
authors.affiliation: College of Chemistry, Sichuan University, Chengdu, PR China. af.lubambo@uol.com.br
authors.forename: Baofeng
authors.initials: B
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Peng
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Ting
authors.initials: T
authors.lastname: Nie
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
mesh_terms: D000873:Anthracenes / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D004247:DNA / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D015316:Genetic Therapy / Q000295:instrumentation / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013464:Sulfuric Acids / Q000737:chemistry; D013997:Time Factors; D014162:Transfection; D014783:Viscosity; D000095322:Anthrones
chemical_list: D000873:Anthracenes; D048271:Chitosan; D004247:DNA; D005227:Fatty Acids; D005690:Galactose; D013464:Sulfuric Acids; C004522:anthrone; D000095322:Anthrones; C033158:sulfuric acid