Evaluation of coronary endothelial function by catheter-type NO sensor in high-fat-diet-induced obese dogs.
BACKGROUND
Direct measurement of plasma nitric oxide (NO) concentration is possible with a newly developed catheter-type sensor.


RESULTS
Adult mongrel dogs (n=5) were fed a high-fat diet (120 kcal . kg(-1) . day(-1)) for 8 months, then endothelial function was assessed by the change in NO concentration induced by acetylcholine (ACh) (DeltaNO). Simultaneously, average peak velocity (APV) was obtained by Doppler guide wire. Although fasting plasma glucose levels did not change after high-fat diet, fasting plasma insulin levels increased significantly (103+/-36 vs 106+/-27 mg/dl, P=0.89 and 0.15+/-0.15 vs 0.26+/-0.07 ng/ml, P=0.04, respectively). ACh-induced peak APV after high-fat feeding was not significantly different from that at baseline (ACh 0.1 microg/kg; 43+/-17 vs 51+/-7 cm/s, P=NS, ACh 0.4 microg/kg; 45+/-20 vs 47+/-16 cm/s, P=NS, respectively). The DeltaNO was significantly smaller after high-fat diet than at baseline (ACh 0.1 microg/kg; 2.6+/-1.6 vs 1.0+/-0.5 nmol/L, P=0.03, ACh 0.4 microg/kg; 3.8+/-2.3 vs 1.8+/-1.1 nmol/L, P=0.04, respectively).


CONCLUSIONS
In high-fat-diet-induced obese dogs NO production was impaired in the early stage when the coronary flow response to ACh may be preserved.
Kume
Teruyoshi
T
Division of Cardiology, Kawasaki Medical School, Kurashiki, Japan.
Kawamoto
Takahiro
T
Okura
Hiroyuki
H
Neishi
Yoji
Y
Hashimoto
Ken
K
Hayashida
Akihiro
A
Watanabe
Nozomi
N
Kanda
Yukiko
Y
Mochizuki
Seiichi
S
Goto
Masami
M
Yoshida
Kiyoshi
K
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D006328:Cardiac Catheterization / Q000295:instrumentation / Q000379:methods*; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000502:physiology*; D004041:Dietary Fats / Q000494:pharmacology; D004285:Dogs; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D007328:Insulin / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology; D014665:Vasodilator Agents / Q000494:pharmacology
D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D014665:Vasodilator Agents; D009569:Nitric Oxide; D000109:Acetylcholine