Evaluation of the binding properties of maghemite nanoparticle surface-coated with meso-2-3-dimercaptosuccinic acid to serum albumin.
In this study the interaction between magnetic nanoparticles (MNPs) surface-coated with meso-2,3-dimercaptosuccinic acid (DMSA) with both bovine serum albumin (BSA) and human serum albumin (HSA) was investigated. The binding of the MNP-DMSA was probed by the fluorescence quenching of the BSA and HSA tryptophan residue. Magnetic resonance and light microscopy analyses were carried out in in vivo tests using female Swiss mice. The binding constants (Kb) and the complex stoichiometries (n) indicate that MNP-DMSA/BSA and MNP-DMSA/HSA complexes have low association profiles. After five minutes following intravenous injection of MNP-DMSA into mice's blood stream we found the lung firstly target by the MNP-DMSA, followed by the liver in a latter stage. This finding suggests that the nanoparticle's DMSA-coating process probably hides the thiol group, through which albumin usually binds. This indicates that biocompatible MNP-DMSA is a very promising material system to be used as a drug delivery system (DDS), primarily for lung cancer treatment.
Simoni
Andreza R
AR
Universidade de São Paulo, FFCLRP-USFP Ribeirão Preto-SP 14040-901, Brazil.
Garcia
Mônica P
MP
Azevedo
Ricardo B
RB
Chaves
Sacha Braun
SB
Lacava
Z G M
ZG
Lima
E C D
EC
Morais
P C
PC
Tedesco
A C
AC
D000327:Adsorption; D001665:Binding Sites; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008280:Magnetics; D008422:Materials Testing; D008968:Molecular Conformation; D050997:Nanomedicine / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D004113:Succimer / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D005290:Ferric Compounds; D046911:Macromolecular Substances; D012709:Serum Albumin; C000499:ferric oxide; D004113:Succimer