title: Identification of CD15 as a marker for tumor-propagating cells in a mouse model of medulloblastoma.
abstract: The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133(+) cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15(+) cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.
authors.affiliation: Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Tracy-Ann
authors.initials: TA
authors.lastname: Read
authors.forename: Marie P
authors.initials: MP
authors.lastname: Fogarty
authors.forename: Shirley L
authors.initials: SL
authors.lastname: Markant
authors.forename: Roger E
authors.initials: RE
authors.lastname: McLendon
authors.forename: Zhengzheng
authors.initials: Z
authors.lastname: Wei
authors.forename: David W
authors.initials: DW
authors.lastname: Ellison
authors.forename: Phillip G
authors.initials: PG
authors.lastname: Febbo
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wechsler-Reya
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor; D001932:Brain Neoplasms / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism*; D008527:Medulloblastoma / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008817:Mice, Mutant Strains; D016513:Mice, SCID; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D000072079:Patched Receptors; D010455:Peptides / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D051792:Basic Helix-Loop-Helix Proteins; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D053823:Hedgehog Proteins; D016256:Lewis X Antigen; D000072079:Patched Receptors; D010455:Peptides; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C500943:Atoh1 protein, mouse; C000606023:PROM1 protein, human; C000606024:Prom1 protein, mouse