title: Identification of a mosaic transposable element of Paracoccus marcusii composed of insertion sequence ISPmar4 (ISAs1 family) and an IS1247a-driven transposable module (TMo).
abstract: A sacB-based trap plasmid was used to clone a mosaic transposable element (TE) in the carotenoid producer Paracoccus marcusii OS22. This element is composed of an insertion sequence (IS) ISPmar4 (ISAs1 family), which contains an inserted functional transposable module (TMo) generated by a copy of IS1247a (of the IS1380 family). Besides IS1247a sequences, the TMo also contains the 3'-end region of a putative alpha/beta hydrolase gene, whose expression might be activated from the P(2) promoter of IS1247a. The identification of this novel TE provides evidence that transposition of TMos may change an IS into a more complex element resembling noncomposite transposons.
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szuplewska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Bartosik
mesh_terms: D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D008969:Molecular Sequence Data; D010230:Paracoccus / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial