title: Molecular cloning and characterization of heat shock protein 70 from Trichinella spiralis.
abstract: A cDNA encoding heat shock protein 70 of Trichinella spiralis (Ts-Hsp70) was identified by immunoscreening the adult T. spiralis cDNA library with rabbit antisera against T. spiralis adult extracts. The open reading frame of Ts-Hsp70 cDNA encoded a 623-amino acid peptide with a predicted molecular weight of 68.7kDa, which shares a high degree of sequence conservation with HSP70s from other parasites. Recombinant Ts-Hsp70 was expressed in Escherichia coli and purified with nickel column chromatography. Western blot analysis showed that recombinant Ts-Hsp70 could be recognized not only by trichinellosis patient sera, but also by T. spiralis-infected sera from rabbits, swine, and mice. Mice vaccinated with recombinant Ts-Hsp70 formulated with Freund's adjuvant exhibited strong humoral immune responses indicated by high titer of IgG antibody and significant muscle larval reduction (37%) after being challenged with T. spiralis larvae. The present results indicate that Ts-Hsp70 is a possible candidate vaccine against T. spiralis infection.
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, 10 Xitoutiao, You An Men, Beijing, PR China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.forename: Xinping
authors.initials: X
authors.lastname: Zhu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gu
authors.forename: Junfei
authors.initials: J
authors.lastname: Wei
authors.forename: Ran
authors.initials: R
authors.lastname: Hao
authors.forename: P
authors.initials: P
authors.lastname: Boireau
authors.forename: Shijuan
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009132:Muscles / Q000469:parasitology; D016366:Open Reading Frames; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013552:Swine; D017160:Trichinella spiralis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D018840:HSP70 Heat-Shock Proteins; D015801:Helminth Proteins; D011994:Recombinant Proteins; D022223:Vaccines, Subunit