title: Screening of endophytic fungi with antithrombotic activity and identification of a bioactive metabolite from the endophytic fungal strain CPCC 480097.
abstract: Fermentation extracts from 1,075 endophytic fungi were screened by the Markwardt method. The endophytic strain CPCC 480097 had the strongest antithrombotic activity and was identified as Fusarium sp. based on morphologic tests and internal transcriptional spacer sequence analysis. The target of the antithrombotic agent from the endophytic strain CPCC 480097 was identified by analysis of the fibrinogen clotting time, amidolytic activity, and fibrinolytic assay. The results showed that this antithrombotic agent is a 28-kDa single-chain fibrinolytic enzyme. The identification of this fibrinolytic enzyme was performed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The two internal sequences of this enzyme were obtained, and these showed no homology with those of other known fibrinolytic enzymes.
authors.affiliation: Department of Biological Pharmacy, Shanghai Institute of Pharmaceutical Industry, Shanghai, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Licheng
authors.initials: L
authors.lastname: Wu
authors.forename: Lingao
authors.initials: L
authors.lastname: Ruan
authors.forename: Mei
authors.initials: M
authors.lastname: Ge
authors.forename: Daijie
authors.initials: D
authors.lastname: Chen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005670:Fusarium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010944:Plants / Q000382:microbiology*; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005343:Fibrinolytic Agents; D005656:Fungal Proteins