title: Parainfluenza virus type 5 (PIV-5) morphology revealed by cryo-electron microscopy.
abstract: The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology.
authors.affiliation: Université de Lyon, F-69000 Lyon, France. olivier.terrier@sante.univ-lyon1.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Terrier
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Rolland
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.forename: Daniel
authors.initials: D
authors.lastname: Thomas
authors.forename: Vincent
authors.initials: V
authors.lastname: Moules
mesh_terms: D020285:Cryoelectron Microscopy; D018098:Paramyxovirinae / Q000737:chemistry / Q000648:ultrastructure*; D010316:Particle Size; D014759:Viral Envelope Proteins / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D014759:Viral Envelope Proteins