title: Enhanced biofilm formation and 3-chlorobenzoate degrading activity by the bacterial consortium of Burkholderia sp. NK8 and Pseudomonas aeruginosa PAO1.
abstract: OBJECTIVE
  To characterize biofilm formation of a chlorobenzoates (CBs) degrading bacterium, Burkholderia sp. NK8, with another bacterial species, and the biodegradation activity against CBs in the mixed-species biofilm.
  
  
  RESULTS
  Burkholderia sp. NK8 was solely or co-cultured with each of five other representative bacteria in microtitre dishes. Biofilm formation involving the strain NK8 was synergistically promoted by co-culturing with only Pseudomonas aeruginosa PAO1. Epifluorescent microscopy revealed that cells of the bacterial strain NK8 were viable and distributed randomly in the mixed-species biofilms. Enumeration of the attached cells on the surface of wells revealed that cells of the strain NK8 increased approx. 10-fold by the co-culture with the strain PAO1 compared to those by monoculture of the strain NK8, and the degradation activity of 3-chlorobenzoate by the dual-species biofilms was more promoted than that by the strain NK8-monocultured biofilms.
  
  
  CONCLUSIONS
  Enhanced biofilm formation of Burkholderia sp. NK8 by the bacterial consortium occurred, but is determined by the partner bacterial species. The mixed-species biofilms have the advantage to degrade CBs on a solid surface.
  
  
  CONCLUSIONS
  This study provides a significance of bacterial consortia on the biofilm formation and the degradation activity of Burkholderia sp. NK8, which contribute for complete degradation of chlorinated aromatics.
authors.affiliation: Biofunction Division, National Institute for Agro-Environmental Sciences, Tsukuba, Japan. yoshige@affrc.go.jp
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: N
authors.initials: N
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: S
authors.initials: S
authors.lastname: Tsushima
mesh_terms: D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D019117:Burkholderia / Q000254:growth & development* / Q000378:metabolism*; D002723:Chlorobenzoates / Q000378:metabolism*; D018920:Coculture Techniques; D015169:Colony Count, Microbial; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D002723:Chlorobenzoates; C036427:3-chlorobenzoic acid