Simplified buccal DNA extraction with FTA Elute Cards.
DNA isolation is the initial step of most genetic studies, and ideally it should use a reliable and non-invasive method. Buccal samples are adequate for such purposes, being painless, easy to collect and a very reliable DNA source. FTA Elute Cards are relatively new on the market and are designed for rapid blood DNA extraction, in which DNA is solubilized in water, instead of attached to the paper matrix. In this study, we sought to test if FTA Elute Cards are suitable for buccal DNA extraction. Furthermore, several time and temperature conditions were analyzed in order to determine the best concentration/time ratio. Twenty-five different conditions were tested in quadruplicate and extracted DNA was quantified by real-time PCR.
Wolfgramm
Eldamária de Vargas
Ede V
Núcleo de Genética Humana e Molecular, Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468 Campus de Maruípe, 29040-090 Vitória, ES, Brazil.
de Carvalho
Fernanda Magri
FM
Aguiar
Vitor Rezende da Costa
VR
Sartori
Mariana Penha De Nadai
MP
Hirschfeld-Campolongo
Gabriela C R
GC
Tsutsumida
Weslley M
WM
Louro
Iúri Drumond
ID
D004247:DNA / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology*; D010209:Paper; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors
D011933:Reagent Kits, Diagnostic; D004247:DNA