title: Integrated oxidative folding of cysteine/selenocysteine containing peptides: improving chemical synthesis of conotoxins.
abstract: Building bridges: The use of diselenide and selectively ((15)N/(13)C)-labeled disulfide bridges is combined to give improvements in oxidative folding and disulfide mapping. Conotoxin analogues, each with a pair of selenocysteines (Sec) and labeled cysteines (see scheme, red), exhibited significantly improved folding and the labeled cysteines allow correctly folded species to be rapidly identified by NMR spectroscopy.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah 84108, USA.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Walewska
authors.forename: Min-Min
authors.initials: MM
authors.lastname: Zhang
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Skalicky
authors.forename: Doju
authors.initials: D
authors.lastname: Yoshikami
authors.forename: Baldomero M
authors.initials: BM
authors.lastname: Olivera
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bulaj
mesh_terms: D020916:Conotoxins / Q000138:chemical synthesis* / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*; D017510:Protein Folding; D017279:Selenocysteine / Q000737:chemistry*
chemical_list: D020916:Conotoxins; D004220:Disulfides; D010455:Peptides; D017279:Selenocysteine; D003545:Cysteine