title: Oct4-induced pluripotency in adult neural stem cells.
abstract: The four transcription factors Oct4, Sox2, Klf4, and c-Myc can induce pluripotency in mouse and human fibroblasts. We previously described direct reprogramming of adult mouse neural stem cells (NSCs) by Oct4 and either Klf4 or c-Myc. NSCs endogenously express Sox2, c-Myc, and Klf4 as well as several intermediate reprogramming markers. Here we report that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs. These one-factor induced pluripotent stem cells (1F iPS) are similar to embryonic stem cells in vitro and in vivo. Not only can these cells can be efficiently differentiated into NSCs, cardiomyocytes, and germ cells in vitro, but they are also capable of teratoma formation and germline transmission in vivo. Our results demonstrate that Oct4 is required and sufficient to directly reprogram NSCs to pluripotency.
authors.affiliation: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, NRW, Germany.
authors.forename: Jeong Beom
authors.initials: JB
authors.lastname: Kim
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sebastiano
authors.forename: Guangming
authors.initials: G
authors.lastname: Wu
authors.forename: Marcos J
authors.initials: MJ
authors.lastname: Araúzo-Bravo
authors.forename: Philipp
authors.initials: P
authors.lastname: Sasse
authors.forename: Luca
authors.initials: L
authors.lastname: Gentile
authors.forename: Kinarm
authors.initials: K
authors.lastname: Ko
authors.forename: David
authors.initials: D
authors.lastname: Ruau
authors.forename: Mathias
authors.initials: M
authors.lastname: Ehrich
authors.forename: Dirk
authors.initials: D
authors.lastname: van den Boom
authors.forename: Johann
authors.initials: J
authors.lastname: Meyer
authors.forename: Karin
authors.initials: K
authors.lastname: Hübner
authors.forename: Christof
authors.initials: C
authors.lastname: Bernemann
authors.forename: Claudia
authors.initials: C
authors.lastname: Ortmeier
authors.forename: Martin
authors.initials: M
authors.lastname: Zenke
authors.forename: Bernd K
authors.initials: BK
authors.lastname: Fleischmann
authors.forename: Holm
authors.initials: H
authors.lastname: Zaehres
authors.forename: Hans R
authors.initials: HR
authors.lastname: Schöler
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D053595:Embryonic Stem Cells / Q000378:metabolism; D005854:Germ Cells / Q000166:cytology; D000090062:Kruppel-Like Factor 4; D016256:Lewis X Antigen / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000378:metabolism*
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; D000469:Alkaline Phosphatase