title: Mitochondrial DNA analysis of Sporothrix schenckii in India, Thailand, Brazil, Colombia, Guatemala and Mexico.
abstract: Up to now, 30 mitochondrial DNA(mtDNA)and 4 rDNA types of Sporothrix schenckii strains have been identified. Here, seventy-six isolates of S. schenckii from Mexico, Guatemala, Brazil, Thailand and India were genotyped and studied epidemiologically by mtDNA restriction fragment length polymorphisms(RFLP)and internal transcribed spacer region(ITS)-RFLP analysis and two new mtDNA types, Type 31 and Type 32, were found. Type 30, previously reported by Mora-Cabrera et al. was confirmed to be Type 3 and designated as blank. Of 48 isolates from Mexico, 41 belonged to Group A wherein Type 2(13 isolates), Type 3(10)and Type 28(7)were dominant. All ten isolates from India and Thailand belonged to Group B. The 52 Group A and 24 Group B isolates corresponded to rDNA Type I and Type IV , respectively, reported by Watanabe et al.(Nippon Ishinkin Gakkai Zasshi 45: 165-175, 2004).
authors.affiliation: Division of Dermatomycology (Novartis Pharma), Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishizaki
authors.forename: Masako
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Kazushi
authors.initials: K
authors.lastname: Anzawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Arunaloke
authors.initials: A
authors.lastname: Chakrabarti
authors.forename: Rataporn
authors.initials: R
authors.lastname: Ungpakorn
authors.forename: Haydee
authors.initials: H
authors.lastname: Torres Guererro
authors.forename: Conchita
authors.initials: C
authors.lastname: Toriello
authors.forename: Robert
authors.initials: R
authors.lastname: Arenas
mesh_terms: D001938:Brazil; D003105:Colombia; D004271:DNA, Fungal / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D006166:Guatemala; D007194:India; D008800:Mexico; D012150:Polymorphism, Restriction Fragment Length; D013175:Sporothrix / Q000145:classification / Q000235:genetics*; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial