Antaxia-telangiectasia mutated gene polymorphisms and susceptibility to chromosomal damage among polycyclic aromatic hydrocarbons exposed workers.
The aim was to explore the relationship between the ataxia-telangiectasia mutated (ATM) gene polymorphisms and susceptibility to cytokinesis-block micronucleus among workers exposed to polycyclic aromatic hydrocarbons (PAH). Blood and urine samples of 140 PAH-exposed workers and 66 non-PAH-exposed workers were collected. Seven tagging single nucleotide polymorphisms in ATM gene were selected by pariwise r(2) method and minor allele frequency cutoff of 0.05 from Chinese genotype data in HapMap project. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphisms of ATM rs600931, rs652311, rs227060, rs227292, rs624366, rs189037 and rs228589. The results showed that ATM rs600931 AG and AG+AA genotypes exhibited significantly higher cytokinesis-block micronucleus (CBMN) frequency (11.14+/-6.91 per thousand and 10.57+/-6.82 per thousand) than did the GG genotype (7.66+/-5.69 per thousand, P=0.015 and 0.038, respectively). The subjects with rs189037 GA and GA+AA genotypes exhibited significantly higher CBMN frequency (10.99+/-6.90 per thousand and 10.51+/-6.76 per thousand) than that of the GG genotype (7.72+/-5.82 per thousand, P=0.018 and 0.035, respectively). The PAH-exposed workers with rs624366 GC and GC+CC genotypes exhibited significantly higher CBMN frequency (11.34+/-6.74 per thousand and 10.73+/-6.62 per thousand) than did the GG genotype (7.61+/-6.07 per thousand, P=0.001 and 0.003, respectively). rs227092 GT genotype exhibited significantly higher CBMN frequency (10.78+/-6.60 per thousand) than did the GG genotype (7.91+/-6.30 per thousand, P=0.025) among the PAH-exposed workers. The haplotype pairs GGGGTGC/AAACATT exhibited significantly higher CBMN frequency (12.05+/-7.40 per thousand) than did the GGGGTGC/GGGGTGC (7.51+/-6.19 per thousand, P=0.007) among the PAH-exposed group. In conclusion, it is suggested that the polymorphisms of ATM were associated with the CBMN frequencies among PAH-exposed workers.
Wang
Yadong
Y
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, PR China.
Duan
Huawei
H
Dai
Yufei
Y
Bin
Ping
P
Cheng
Juan
J
Pan
Zufei
Z
Huang
Chuanfeng
C
Leng
Shuguang
S
Zheng
Yuxin
Y
D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000235:genetics*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011110:Polymorphism, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases