[Identification of EBV chromosomal integration sites in Raji cells by fluorescence in situ hybridization].
OBJECTIVE
To identify the Epstein-Barr virus (EBV) chromosomal integration sites in Raji cells.


METHODS
EBV DNA was detected by Southern hybridization, and the viral chromosomal integration sites were identified using G banding and fluorescence in situ hybridization (FISH).


RESULTS
BamHI-digested genomic DNA from Raji cells was hybridized with (32)P-labeled probe-1 (EBV genome 13,232 approximately 16,189) and Probe-2 (EBV genome 5 approximately 3,271), which generated 4 and 10, 23 kb positive bands respectively. The viral integration sites included 1p, 1q, 2q, 3p, 3q, 4 q, 5q, 6q, 7p, 7q, 9q,11p, 14 q, and 15q,and chromosomal bands 4 q, 2q, 1q and 7q were viral integration sites with high frequencies. Among the 33 signals counted, 7, 4, 4,and 4 signals were at the site 4 q, 2q, 1q, and 7q respectively, and 64% of the total signals were found in these 4 chromosomal bands. No viral integration occurred in chromosomes 16 approximately 22 or the sex chromosomes (X, Y).


CONCLUSIONS
This study firstly identifies the EBV integration sites in Raji cells using G banding and FISH. There are some viral integration sites with high frequencies in Raji cells, and EBV integrates into Raji cell genomes non-randomly.
Gao
Jianming
J
Department of Pathophysiology, School of Medicine, Three Gorges University, Yichang Hubei 443002,China.
Li
Xiaoling
X
Li
Guiyuan
G
D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology / Q000821:virology*; D045744:Cell Line, Tumor; D002878:Chromosomes, Human, Pair 1 / Q000821:virology; D002889:Chromosomes, Human, Pair 2 / Q000821:virology; D002894:Chromosomes, Human, Pair 4 / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016662:Virus Integration / Q000235:genetics*
D004279:DNA, Viral