title: The anti-condyloma acuminatum effects of interferon-inducible protein 10 in vitro.
abstract: BACKGROUND
  Interferon-inducible protein 10 (IP-10) has been reported to show an effective antiviral and antineoplastic role in various murine models.
  
  
  OBJECTIVE
  To investigate the anti-condyloma acuminatum effect and human papillomavirus (HPV)-inhibiting efficacy of a recombinant plasmid encoding IP-10 in vitro.
  
  
  METHODS
  A recombinant plasmid DNA carrying IP-10 cDNA was constructed. Condyloma acuminatum tissue particles were transfected with IP-10 and examined for apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry techniques. Relative real-time polymerase chain reaction (PCR) was used to validate the HPV inhibited level of the treatment groups. Immunohistochemistry was used to determine the IP-10 expression on condyloma acuminatum tissues.
  
  
  RESULTS
  Transfected IP-10 was expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful apoptosis was observed in condyloma acuminatum tissues transfected with IP-10. In addition, HPV expression was lower in IP-10-treated tissues than in control tissues.
  
  
  CONCLUSIONS
  These observations suggest that IP-10 has strong anti-condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and therefore may be a novel and potentially effective therapy for condyloma acuminatum.
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhao
authors.forename: Pin
authors.initials: P
authors.lastname: Chen
authors.forename: Chenxian
authors.initials: C
authors.lastname: Cheng
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000328:Adult; D017209:Apoptosis; D054357:Chemokine CXCL10 / Q000235:genetics*; D003218:Condylomata Acuminata / Q000628:therapy* / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D052140:Human papillomavirus 11; D052163:Human papillomavirus 6; D006801:Humans; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D011994:Recombinant Proteins