Detection of infectious bronchitis virus in experimentally infected chickens by an antigen-competitive ELISA.
An antigen-competitive enzyme-linked immunosorbent assay (Ag-C-ELISA) was developed for the detection of infectious bronchitis virus (IBV) antigens, M41 strain, in tissues from experimentally infected chickens, or in allantoic fluid harvested from inoculated embryonated eggs. The detection limit of IBV in the Ag-C-ELISA was 10(4.1) median embryo infective doses (EID(50))/well. Tracheal and lung samples from chickens vaccinated with 10(2.5) EID(50) of live attenuated infectious bronchitis (H120) vaccine were negative in the direct detection Ag-C-ELISA. The results indicate that the Ag-C-ELISA has the potential to detect IBV, either directly in tissue samples or when combined with the passage of material in embryonated eggs, thereby constituting an alternative method for the diagnosis of IBV.
Bronzoni
R V
RV
Universidade Estadual Paulista, Faculdade de Cieinsertion markncias Agrarias e Veterinarias, Departmento de Patologia Veterinaria - Laboratorio de Imunologia e Virologia, Rodovia Prof. Paulo Donatto Castellani, Km 05, 14870- 000, Jaboticabal, SP, Brazil.
Pinto
A A
AA
Montassier
H J
HJ