title: Myosin-IIA and ICAM-1 regulate the interchange between two distinct modes of T cell migration.
abstract: How T cells achieve rapid chemotactic motility under certain circumstances and efficient cell surface surveillance in others is not fully understood. We show that T lymphocytes are motile in two distinct modes: a fast "amoeboid-like" mode, which uses sequential discontinuous contacts to the substrate; and a slower mode using a single continuously translating adhesion, similar to mesenchymal motility. Myosin-IIA is necessary for fast amoeboid motility, and our data suggests that this occurs via cyclical rear-mediated compressions that eliminate existing adhesions while licensing subsequent ones at the front of the cell. Regulation of Myosin-IIA function in T cells is thus a key mechanism to regulate surface contact area and crawling velocity within different environments. This can provide T lymphocytes with motile and adhesive properties that are uniquely suited toward alternative requirements for immune surveillance and response.
authors.affiliation: Department of Pathology, University of California San Francisco, San Francisco CA 94143, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Jacobelli
authors.forename: F Chris
authors.initials: FC
authors.lastname: Bennett
authors.forename: Priya
authors.initials: P
authors.lastname: Pandurangi
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Tooley
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Krummel
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000276:immunology; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology*; D015151:Immunoblotting; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D024507:Nonmuscle Myosin Type IIA / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
chemical_list: D018799:Intercellular Adhesion Molecule-1; D024507:Nonmuscle Myosin Type IIA