Inert matrix and Na 4 EDTA improve the supercritical fluid extraction efficiency of fluoroquinolones for HPLC determination in pig tissues.
A supercritical fluid extraction method combined with high-performance liquid chromatography-fluorescence detection was developed for the determination of enrofloxacin, danofloxacin, and ciprofloxacin in pig muscle, lung, and kidney samples. The optimal SFE conditions were 80 degrees C, 300 kg/cm(2), 30% methanol for 40 min as a dynamic extraction time, in addition to 0.2g Na(4)EDTA and 7.0 g sea sand in the extraction vessel. The use of Na(4)EDTA and sea sand on SFE extraction resulted in improvement of the recoveries of ciprofloxacin, a polar and hydrophilic compound, as well as enrofloxacin and danofloxacin. Overall, the recoveries ranged from 86.7 to 113.1% using the Na(4)EDTA/sea sand-assisted SFE extraction method. The Na(4)EDTA/sea sand-assisted SFE-HPLC-FLD validated method was successfully carried out in pig tissues, and proved to be specific, sensitive, reliable, and accurate. The method was also applied satisfactorily for accurate quantitative residue analysis in incurred pig tissues.
Choi
Jeong-Heui
JH
Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
Mamun
M I R
MI
Abd El-Aty
A M
AM
Kim
Kyung Tae
KT
Koh
Hong-Bum
HB
Shin
Ho-Chul
HC
Kim
Jin-Suk
JS
Lee
Kang Bong
KB
Shim
Jae-Han
JH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis / Q000302:isolation & purification; D004492:Edetic Acid; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis / Q000302:isolation & purification*; D007668:Kidney / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D034421:Sus scrofa
D024841:Fluoroquinolones; C068581:danofloxacin; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D004492:Edetic Acid