Continuous counter-current extraction on an industrial sample using dual-flow counter-current chromatography.
Both batch and continuous separations were performed on an industrial liquor using a specially built continuous counter-current extraction centrifuge. Changing the flow regime for different batch separations showed that the elution of components from the respective ends of the coil depends on the flow rates of both upper and lower phases. It was shown that, within the scope of the study, the elution of the components was not affected by the concentration of the injected reaction liquor and more importantly that continuous processing with a counter-current chromatography centrifuge was feasible. This research represents an important step forward in making continuous counter-current chromatography (or true moving bed chromatography) accessible for the pharmaceutical industry.
van den Heuvel
Remco
R
Brunel Institute for Bioengineering, Brunel University, Uxbridge, UK. remco.van.den.heuvel@brunel.ac.uk
Mathews
Ben
B
Dubant
Stephane
S
Sutherland
Ian
I
D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid; D045424:Complex Mixtures / Q000737:chemistry; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods; D004345:Drug Industry; D004867:Equipment Design; D000432:Methanol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000302:isolation & purification
D045424:Complex Mixtures; D004364:Pharmaceutical Preparations; D000432:Methanol