title: Dynamics of microtubules, motor proteins and 20S proteasomes during bovine oocyte IVM.
abstract: The present study investigated the distribution of cytoplasmic dynein, dynactin and 20S proteasomes in oocytes isolated from small (<2 mm) and large (2-8 mm) follicles during IVM. Immediately after chromatin condensation (germinal vesicle (GV) breakdown), dynactin was closely associated with the chromatin and interacted with tubulin at the MI and MII spindles in oocytes recovered from large follicles. Dynactin showed perinuclear concentration. Dynein was homogeneously distributed in the cytoplasm of GV oocytes in both groups and was associated with the chromatin at the MI and MII spindle. The 20S proteasomes were found predominantly in the nucleus at the GV stage and were associated with the chromatin up to the MII stage in both groups of oocytes. The use of sodium orthovanadate, an inhibitor or phosphatase and ATPase activity, and nocodazole, a known disruptor of microtubules, affected the localisation of proteasomes in the meiotic stages. The results demonstrate the distinct dynamics of molecular motors and proteasomes during bovine oocyte IVM, their possible relationship with the developmental competence of the oocyte and the link between microtubules, their associated molecular motors and the transport of proteasomes during bovine female meiosis.
authors.affiliation: Laboratorio de Biotecnología Animal, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martin 4453 (C1417DSE), Buenos Aires, Argentina.
authors.forename: S E
authors.initials: SE
authors.lastname: Racedo
authors.forename: M C
authors.initials: MC
authors.lastname: Branzini
authors.forename: D
authors.initials: D
authors.lastname: Salamone
authors.forename: C
authors.initials: C
authors.lastname: Wójcik
authors.forename: V Y
authors.initials: VY
authors.lastname: Rawe
authors.forename: H
authors.initials: H
authors.lastname: Niemann
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly; D003593:Cytoplasm / Q000378:metabolism; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D008540:Meiosis / Q000187:drug effects; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology*; D009866:Oogenesis / Q000187:drug effects; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism; D050257:Tubulin Modulators / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
chemical_list: D000072159:Dynactin Complex; D004791:Enzyme Inhibitors; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D014404:Tubulin; D050257:Tubulin Modulators; D014638:Vanadates; D046988:Proteasome Endopeptidase Complex; D004398:Dyneins; D015739:Nocodazole