Real time investigation of protein folding, structure, and dynamics in living cells.
Real time investigation of protein folding, structure, and dynamics at high resolution in living cells, is the next major step in nano cell biology. However, there are a number of major hurdles that need to be overcome, such as coupled translation and folding, the intervention of chaperones and folding enzymes, translocation, export, and the targeting machinery, proteasomes and degradation signals, etc. Although some progress has been made in recent years (Royer, 2006), current technology available in the field is limited for an enhanced resolution study, and no major breakthrough has been achieved in this direction. The major challenge is to develop new approaches that permit high-resolution, direct, and accurate structural measurements of protein folding and structure, in specific compartments of living cells. In this chapter, we focus on a review of a QQ-reagent based protein transduction recently developed in our laboratory technology that may allow us to develop a high-resolution in vivo investigation of protein folding, structure, and dynamics.
Li
Qianqian
Q
Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine Detroit, Michigan 48021, USA.
Huang
Yuefei
Y
Xiao
Nan
N
Murray
Victoria
V
Chen
Jianglei
J
Wang
Jianjun
J
D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism; D001057:Apolipoproteins E / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D007202:Indicators and Reagents / Q000378:metabolism; D042541:Intracellular Space / Q000201:enzymology; D051379:Mice; D018832:Molecular Chaperones / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D012015:Reference Standards; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods*
D016632:Apolipoprotein A-I; D001057:Apolipoproteins E; D007202:Indicators and Reagents; D018832:Molecular Chaperones; D049452:Green Fluorescent Proteins; D010447:Peptide Hydrolases