title: The immunosuppressive activity and solution structures of ubiquitin fragments.
abstract: Recently, ubiquitin was suggested as a promising anti-inflammatory protein therapeutic. We found that a peptide fragment corresponding to the ubiquitin(50-59) sequence (LEDGRTLSDY) possessed the immunosuppressive activity comparable with that of ubiquitin. CD and NMR spectroscopies were used to determine the conformational preferences of LEDGRTLSDY in solution. The peptide mixture, obtained by pepsin digestion of ubiquitin, was even more potent than the intact protein. Although the peptide exhibited a well-defined conformation in methanol, its structure was distinct from the corresponding 50-59 fragment in the native ubiquitin molecule. (c) 2009 Wiley Periodicals, Inc. Biopolymers 91: 423-431, 2009.
authors.affiliation: Faculty of Chemistry, University of Wrocław, Wrocław, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Jaremko
authors.forename: Mariusz
authors.initials: M
authors.lastname: Jaremko
authors.forename: Paweł
authors.initials: P
authors.lastname: Pasikowski
authors.forename: Marek
authors.initials: M
authors.lastname: Cebrat
authors.forename: Piotr
authors.initials: P
authors.lastname: Stefanowicz
authors.forename: Marek
authors.initials: M
authors.lastname: Lisowski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Artym
authors.forename: Michał
authors.initials: M
authors.lastname: Zimecki
authors.forename: Igor
authors.initials: I
authors.lastname: Zhukov
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Szewczuk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002942:Circular Dichroism; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000737:chemistry; D010446:Peptide Fragments; D012756:Sheep; D012996:Solutions / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D007166:Immunosuppressive Agents; D010446:Peptide Fragments; D012996:Solutions; D025801:Ubiquitin; D010434:Pepsin A