PKD2 interacts with Lck and regulates NFAT activity in T cells.
Protein kinase D2 (PKD2) is a member of the PKD serine/threonine protein kinase family that has been implicated in the regulation of a variety of cellular processes including proliferation, survival, protein trafficking and immune response. In the present study, we report a novel interaction between PKD2 and Lck, a member of the Src tyrosine protein kinase family that is predominantly expressed in T cells. This interaction involved the C-terminal kinase domains of both PKD2 and Lck. Moreover, co-expression of Lck enhanced the tyrosine phosphorylation of PKD2 and increased its kinase activity. Finally, we report that PKD2 enhanced T cell receptor (TCR)-induced nuclear factor of T cell (NFAT) activity in Jurkat T cells. These results suggested that Lck regulated the activity of PKD2 by tyrosine phosphorylation, which in turn may have modulated the physiological functions of PKD2 during TCR-induced T cell activation. [BMB reports 2009; 42(1): 35-40].
Li
Qing
Q
Department of Life Science and Biotechnology, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai, 200030, China.
Sun
Xiaoqing
X
Wu
Jun
J
Lin
Zhixin
Z
Luo
Ying
Y
D017930:Genes, Reporter; D006801:Humans; D019169:Jurkat Cells; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000737:chemistry / Q000378:metabolism*; D050778:NFATC Transcription Factors / Q000378:metabolism*; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D000091283:Protein Kinase D2; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism
D050778:NFATC Transcription Factors; D000091283:Protein Kinase D2; D011948:Receptors, Antigen, T-Cell; D018808:Transcription Factor AP-1; D019000:Phosphotyrosine; D011494:Protein Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)