title: Helicobacter pylori enhances cyclooxygenase 2 expression via p38MAPK/ATF-2 signaling pathway in MKN45 cells.
abstract: The over-expression of COX-2 (Cyclooxygenase 2) protein has been reported to play a key role in the incidence and development of Helicobacter pylori-associated gastric cancer. The induction of COX-2 in the gastric cancer cells with H. pylori has been demonstrated previously, but little is known about the mechanism. This study reported that the COX-2 mRNA and proteins expression level and the activity of COX-2 promoter increased remarkably with H. pylori stimulation in the MKN45 gastric cancer cells. H. pylori also stimulated phosphorylation of p38MAPK and ATF-2, which is the downstream kinase of p38MAPK. Moreover, the expression levels of COX-2 were suppressed with p38MAPK inhibitor treatment. These results suggest that H. pylori-induced activation of p38MAPK/ATF-2-mediated signal pathway is necessary for the expression of COX-2.
authors.affiliation: Department of Oncology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Ningning
authors.initials: N
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yiqin
authors.initials: Y
authors.lastname: Wang
authors.forename: Jue
authors.initials: J
authors.lastname: Sun
authors.forename: Zhongze
authors.initials: Z
authors.lastname: Fan
authors.forename: Rui Hai
authors.initials: RH
authors.lastname: Liu
mesh_terms: D051697:Activating Transcription Factor 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D016481:Helicobacter Infections / Q000150:complications; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D010766:Phosphorylation; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D051697:Activating Transcription Factor 1; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases