Ultrastructural features of lung fibroblast differentiation into myofibroblasts.
Fibroblast differentiation into myofibroblast in transforming growth factor-beta1-exposed human lung fibroblasts and the immunolocalizations of alpha-smooth muscle actin, fibronectin, tenascin-C, and osteopontin in exposed cells were studied by conventional transmission electron microscopy and immunoelectron microscopy. Ultrastructural features of myofibroblasts were detected after exposure, e.g., alpha-smooth muscle actin positive bundles in the cytoplasm of cells and extracellular fibronectin-containing structures on the surface of the cell forming fibronexus structure, osteopontin adjacent to rough endoplastic reticulum and extracellular tenascin-C in the vicinity of the cell. The authors concluded that exposure to transforming growth factor-beta1 can differentiate lung fibroblasts into ultrastructurally typical myofibroblasts.
Kaarteenaho-Wiik
Riitta
R
Institute of Clinical Medicine, Department of Internal Medicine, Centre of Excellence in Research, University of Oulu and Oulu University Hospital, Oulu, Finland. Riitta.Kaarteenaho-Wiik@oulu.fi
Paakko
Paavo
P
Sormunen
Raija
R
D000199:Actins / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008171:Lung Diseases / Q000473:pathology; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D053495:Osteopontin / Q000096:biosynthesis; D019063:Tenascin / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D000199:Actins; D005353:Fibronectins; D019063:Tenascin; D053773:Transforming Growth Factor beta1; D053495:Osteopontin