Engineering a uranyl-specific binding protein from NikR.
A new pick-up line: The first uranyl-selective DNA-binding protein is designed using the E. coli nickel(II)-responsive protein NikR as the template. The resulting NikR' protein binds uranyl (see picture) with a dissociation constant K(d) = 53 nM and selectively binds to DNA in the presence of uranyl.
Wegner
Seraphine V
SV
Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
Boyaci
Hande
H
Chen
Hao
H
Jensen
Mark P
MP
He
Chuan
C
D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009532:Nickel; D015202:Protein Engineering; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014501:Uranium / Q000737:chemistry*
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D050505:Mutant Proteins; C402409:NikR protein, E coli; D012097:Repressor Proteins; D014501:Uranium; D009532:Nickel; D004247:DNA