title: Neurotensin-lmmunoreactive Neurons in the Rat Medial Preoptic Area are Oestrogen-Receptive.
abstract: The identity of neurons influenced by oestrogen is critical for the understanding of ovarian steroid actions in the brain. The medial preoptic area (MPOA) contains one of the largest oestrogen-receptive cell populations in the rat brain and participates in several oestrogen-dependent functions, including the regulation of luteinizing hormone (LH) secretion and sexual behaviour. Using double immunostaining procedures with antibodies specific for the oestrogen receptor and neurotensin, a neuropeptide implicated in the regulation of LH secretion within this area, we found that approximately half of the neurotensin-immunoreactive neurons in the MPOA also displayed immunoreactivity for the oestrogen receptor. We estimate that oestrogen-receptive neurotensin neurons represent 5% to 12% of all oestrogen receptor-positive cells in the MPOA. Our results provide morphological evidence that neurotensin mediates oestrogen-dependent mechanisms within the brain and suggest that oestrogen may act through preoptic neurotensin neurons to aid in the generation of the LH surge.
authors.affiliation: Laboratoire de Neuroendocrinologie Morphofonctionnelle, Université de Bordeaux II, 146 rue Leo Saignat, 33076 Bordeaux Cedex, France.
authors.forename: A E
authors.initials: AE
authors.lastname: Herbison
authors.forename: D T
authors.initials: DT
authors.lastname: Theodosis