title: High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research.
abstract: BACKGROUND
  The annual fish Nothobranchius furzeri is the vertebrate with the shortest known life span in captivity. Fish of the GRZ strain live only three to four months under optimal laboratory conditions, show explosive growth, early sexual maturation and age-dependent physiological and behavioral decline, and express aging related biomarkers. Treatment with resveratrol and low temperature significantly extends the maximum life span. These features make N. furzeri a promising new vertebrate model for age research.
  
  
  RESULTS
  To contribute to establishing N. furzeri as a new model organism, we provide a first insight into its genome and a comparison to medaka, stickleback, tetraodon and zebrafish. The N. furzeri genome contains 19 chromosomes (2n = 38). Its genome of between 1.6 and 1.9 Gb is the largest among the analyzed fish species and has, at 45%, the highest repeat content. Remarkably, tandem repeats comprise 21%, which is 4-12 times more than in the other four fish species. In addition, G+C-rich tandem repeats preferentially localize to centromeric regions. Phylogenetic analysis based on coding sequences identifies medaka as the closest relative. Genotyping of an initial set of 27 markers and multi-locus fingerprinting of one microsatellite provides the first molecular evidence that the GRZ strain is highly inbred.
  
  
  CONCLUSIONS
  Our work presents a first basis for systematic genomic and genetic analyses aimed at understanding the mechanisms of life span determination in N. furzeri.
authors.affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Beutenbergstr,, 07745 Jena, Germany. kathrinr@fli-leibniz.de
authors.forename: Kathrin
authors.initials: K
authors.lastname: Reichwald
authors.forename: Chris
authors.initials: C
authors.lastname: Lauber
authors.forename: Indrajit
authors.initials: I
authors.lastname: Nanda
authors.forename: Jeanette
authors.initials: J
authors.lastname: Kirschner
authors.forename: Nils
authors.initials: N
authors.lastname: Hartmann
authors.forename: Susanne
authors.initials: S
authors.lastname: Schories
authors.forename: Ulrike
authors.initials: U
authors.lastname: Gausmann
authors.forename: Stefan
authors.initials: S
authors.lastname: Taudien
authors.forename: Markus B
authors.initials: MB
authors.lastname: Schilhabel
authors.forename: Karol
authors.initials: K
authors.lastname: Szafranski
authors.forename: Gernot
authors.initials: G
authors.lastname: Glöckner
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.forename: Alessandro
authors.initials: A
authors.lastname: Cellerino
authors.forename: Manfred
authors.initials: M
authors.lastname: Schartl
authors.forename: Christoph
authors.initials: C
authors.lastname: Englert
authors.forename: Matthias
authors.initials: M
authors.lastname: Platzer
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003080:Cold Temperature; D005399:Fishes; D005819:Genetic Markers; D016678:Genome / Q000235:genetics*; D008136:Longevity / Q000235:genetics*; D023421:Models, Animal; D010802:Phylogeny; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D020080:Tandem Repeat Sequences
chemical_list: D000975:Antioxidants; D005819:Genetic Markers; D013267:Stilbenes; D000077185:Resveratrol