Unexpected expression pattern of tetracycline-regulated transgenes in mice.
In generating a conditional transgenic murine model based on a tetracycline-regulated system, we obtained unexpected patterns of expression due to the transcriptional inactivity of the tet-responder promoter. Here we show strong cell-type-restricted expression that was variegated to an extent determined by the number of responder transgene copies integrated into the host genome.
Bao-Cutrona
M
M
Department of Animal Biology-Anthropology, Faculty of Biology, University of Barcelona, Barcelona, Spain. meritxell@negrisud.it
Moral
P
P
D000818:Animals; D018628:Gene Dosage / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009832:Olfactory Nerve / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013752:Tetracycline / Q000494:pharmacology*; D019076:Transgenes / Q000187:drug effects*
D011500:Protein Synthesis Inhibitors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D013752:Tetracycline