title: Highly efficient, selective and sensitive molecular screening of acetylcholinesterase inhibitors of natural origin by solid-phase extraction-liquid chromatography/electrospray ionisation-octopole-orthogonal acceleration time-of-flight-mass spectrometry and novel thin-layer chromatography-based bioautography.
abstract: Highly efficient, selective and sensitive molecular screening of natural acetylcholinesterase (AChE) inhibitors was developed and comprised optimized pressurized liquid extraction (PLE) of plant materials followed by highly selective solid-phase extraction (SPE) using Oasis HLB cartridges. Pure alkaloidal fractions were analyzed by a newly developed high-performance liquid-chromatography (HPLC) on a 3 microm Atlantis HILIC silica stationary phase combined with recently introduced electrospray ionisation (ESI) octopole-orthogonal acceleration time-of-flight (oa TOF)-mass spectrometry (MS) with high mass accuracy (about 2 ppm) and high sensitivity (absolute limit of detection (LOD) for galanthamine was about 43 fg at signal-to-noise 13:1). Moreover, a newly developed and validated TLC-bioautography permit galanthamine sensitivities at pg levels. In this way, more potent than galanthamine AChE inhibitor namely 1,2-dihydrogalanthamine in Narcissus jonquilla 'Pipit' extract could be found (with IC(50) value 0.19 microM lower of about 42% than that of galanthamine).
authors.affiliation: Department of Pharmacognosy with Medicinal Plant Laboratory Unit, Lublin, Poland. tmroczek@pharmacognosy.org
authors.forename: Tomasz
authors.initials: T
authors.lastname: Mroczek
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000032:analysis* / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D031407:Crinum / Q000737:chemistry; D003720:Densitometry / Q000379:methods; D027801:Galanthus / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D031427:Narcissus / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D047151:Amaryllidaceae Alkaloids; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts