De Novo identification of single nucleotide mutations in Caenorhabditis elegans using array comparative genomic hybridization.
Array comparative genomic hybridization (aCGH) has been used primarily to detect copy-number variants between two genomes. Here we report using aCGH to detect single nucleotide mutations on oligonucleotide microarrays with overlapping 50-mer probes. This technique represents a powerful method for rapidly detecting novel homozygous single nucleotide mutations in any organism with a sequenced reference genome.
Maydan
Jason S
JS
Department of Zoology, University of British Columbia V6T 1Z4, Vancouver, British Columbia, Canada.
Okada
H Mark
HM
Flibotte
Stephane
S
Edgley
Mark L
ML
Moerman
Donald G
DG
D000465:Algorithms; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D055028:Comparative Genomic Hybridization / Q000379:methods*; D030541:Databases, Genetic; D049751:Genome, Helminth; D023281:Genomics / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012984:Software