An inducible HSP70 gene from the midge Chironomus dilutus: characterization and transcription profile under environmental stress.
In the present study, we identified and characterized an inducible heat shock protein 70 (HSP70) from the midge Chironomus dilutus and investigated the transcriptional profile of the gene under baseline and environmentally stressful conditions. Using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), we observed increased expression of CD-HSP70-1 in response to both heat shock and copper stress. We also investigated the expression of this gene during midge development. All C. dilutus developmental stages expressed CD-HSP70-1 under normal conditions, although at extremely low levels. Phylogenetic analysis of the amino acid sequence demonstrated distinct clustering of this gene with inducible HSP70s from other insect species.
Karouna-Renier
N K
NK
Center for Environmental Diagnostics and Bioremediation, University of West Florida, 11000 University Parkway, Pensacola, FL 32514-5751, USA. nkarouna@usgs.gov
Rao
K Ranga
KR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D003300:Copper / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology; D013312:Stress, Physiological
D018840:HSP70 Heat-Shock Proteins; D003300:Copper