Construction of arginine-rich peptide displaying bionanocapsules.
Bionanocapsule (BNC) is a hollow nanoparticle composed of L-protein of the hepatitis B virus surface antigen. BNC can deliver genes or drugs into specific human hepatocytes, but delivery is limited to hepatocytes. In this study, we attempted to alter the specificity of BNCs by genetically introducing cell-penetrating peptides (CPPs), such as arginine-rich peptides, into BNCs. The CPP-fused BNC was efficiently internalized into various cell lines in a short period without significant cytotoxicity. These results show that CPP-BNC could be applied as an efficient carrier for gene and drug delivery.
Shishido
Takuya
T
Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Yonezawa
Daisaku
D
Iwata
Kiyokazu
K
Tanaka
Tsutomu
T
Ogino
Chiaki
C
Fukuda
Hideki
H
Kondo
Akihiko
A
D000818:Animals; D001120:Arginine / Q000138:chemical synthesis* / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004331:Drosophila melanogaster; D004337:Drug Carriers / Q000138:chemical synthesis / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D006367:HeLa Cells; D022781:Hepatocytes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry* / Q000821:virology; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism
D004337:Drug Carriers; C096362:L protein, hepatitis B virus; D053769:Nanocapsules; D010455:Peptides; D014759:Viral Envelope Proteins; D001120:Arginine