Effects of 1-bromopropane, a substitute for chlorofluorocarbons, on BDNF expression.
1-Bromopropane (1-BP) has been widely used as an alternative to ozone-depleting chlorofluorocarbons in various industries. Although the neurotoxicity of 1-BP has been recently reported, there is little information about the effect of 1-BP on the cells in brain by experimental approach. Here we studied the effect of 1-BP on brain-derived neurotrophic factor (BDNF) expression in astrocytes in vitro. The BDNF mRNA level was remarkably decreased by 1-BP in a human astrocytoma cell line, U251, and in mouse primary astrocytes. The DNA-binding and specific reporter activity of cAMP response element-binding transcription factor (CREB), which is one of the key molecules regulating BDNF expression, were reduced by 1-BP in U251 and/or mouse primary astrocytes. Additionally, protein kinase A (PKA) activity was suppressed by 1-BP in U251. These results suggest that BDNF expression was affected by 1-BP through at least PKA.
Yoshida
Yasuhiro
Y
Department of Immunology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan. freude@med.uoeh-u.ac.jp
Nakano
Yoshiteru
Y
Ueno
Susumu
S
Liu
Jiqin
J
Fueta
Yukiko
Y
Ishidao
Toru
T
Kunugita
Naoki
N
Yanagihara
Nobuyuki
N
Sugiura
Tsutomu
T
Hori
Hajime
H
Yamashita
Uki
U
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL
D019208:Brain-Derived Neurotrophic Factor; D017362:Cyclic AMP Response Element-Binding Protein; D006842:Hydrocarbons, Brominated; D017868:Cyclic AMP-Dependent Protein Kinases; C118559:1-bromopropane