[Isolation, differentiation and biochemical aspects of amniotic fluid stem cell].
The mesenchymals stem cells (MSCs) are cells with the great potential of differentiation are being introduced in the clinic for treatment of several diseases. Mesenchymal stem cells have several advantages including the stability of their phenotype in vitro.


BACKGROUND
isolation of MSCs in amniotic fluid, its expansion and the demonstration of the capacity of these cells to differentiate in adipogenic and myogenic cells, without to change the chromosomal stability of the MSCs in culture.


METHODS
in order to evaluate the functional change of these cells, were gotten values of the differentiated adipogenic cells and not differentiated through the dosage of triglycerides. The myogenic nature of the differentiated cells was analyzed comparing the creatine kinase--CK, lactic dehydrogenase--LDH and aldolase produced by the cells.


RESULTS
the values of triglycerides were significantly higher in differentiated cells, showing intracytoplasmatic granule form after differentiation. All the biochemical characters were significantly higher in differentiated myogenic cells.


CONCLUSIONS
this study suggests that the standardized protocol of differentiation can be used in the attainment of cells with characteristics of adipogenic and muscular cells, from amniotic fluid.
Cabral
Antônio Carlos Vieira
AC
Departamento de Genética Humana e pela Criovida do Instituto H. Pardini, Hospital das Clínicas, Faculdade de Farmácia da Universidade Federal de Minais Gerais, Belo Horizonte, MG.
Angelo
Patrícia Caroline
PC
Leite
Henrique Vitor
HV
Pereira
Alamanda Kfouri
AK
Lopes
Ana Paula Brum Miranda
AP
Oliveira
Maria Beatriz de
MB
Borges
Karina Braga Gomes
KB
Pardini
Victor Cavalcanti
VC
Ferreira
Alessandro Clayton de Sousa
AC
D000653:Amniotic Fluid / Q000166:cytology* / Q000201:enzymology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000032:analysis*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011247:Pregnancy; D014280:Triglycerides / Q000097:blood
D014280:Triglycerides; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase