Expression of decorin in esophageal cancer in relation to the expression of three isoforms of transforming growth factor-beta (TGF-beta1, -beta2, and -beta3) and matrix metalloproteinase-2 activity.
To determine the role of the reactive stroma in cancer progression, we investigated decorin (DCN) and transforming growth factor-beta (TGF-beta expression, and matrix metalloproteinase-2 (MMP-2) activity in the tumorous esophagus. We found statistically insignificantly decreased levels of DCN expression in the pathological tissues. No obvious alterations in TGF-beta expression were noticed. The highly significant increase in MMP-2 activity in cancers did not result in elevated levels of TGF-beta dimers. Therefore, the system of TGF-beta liberation from its complex with DCN by activated MMP-2 does not seem to contribute to esophageal cancerogenesis, although this hypothesis should be reevaluated with a larger study group.
Augoff
K
K
Department of Gastrointestinal and General Surgery, University of Wroclaw, Poland. augoffka@chir.am.wroc.pl
Grabowski
K
K
Rabczynski
J
J
Kolondra
A
A
Tabola
R
R
Sikorski
A F
AF
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D058575:Decorin; D004789:Enzyme Activation; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D020033:Protein Isoforms; D011509:Proteoglycans / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D053781:Transforming Growth Factor beta2 / Q000032:analysis; D053782:Transforming Growth Factor beta3 / Q000032:analysis
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D020033:Protein Isoforms; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2