Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions.
Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P <0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P=0.001 and P=0.014, respectively).
Antonio
Martin
M
Bacterial Diseases Programme, Medical Research Council (MRC) Laboratories, Banjul, The Gambia.
Hakeem
Ishrat
I
Bacterial Diseases Programme, Medical Research Council (MRC) Laboratories, Banjul, The Gambia.
Sankareh
Kawsu
K
Bacterial Diseases Programme, Medical Research Council (MRC) Laboratories, Banjul, The Gambia.
Cheung
Yin Bun
YB
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK.
Adegbola
Richard A
RA
Bacterial Diseases Programme, Medical Research Council (MRC) Laboratories, Banjul, The Gambia.
D016010:Binomial Distribution; D002353:Carrier State / Q000382:microbiology*; D003430:Cross-Sectional Studies; D004269:DNA, Bacterial / Q000302:isolation & purification; D005714:Gambia; D006801:Humans; D007841:Latex Fixation Tests; D009305:Nasopharynx / Q000382:microbiology*; D011008:Pneumococcal Infections / Q000382:microbiology*; D022242:Pneumococcal Vaccines / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods*; D011336:Probability; D012680:Sensitivity and Specificity; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018074:Vaccines, Conjugate / Q000592:standards
D004269:DNA, Bacterial; D022242:Pneumococcal Vaccines; D018074:Vaccines, Conjugate