title: [Differentiantion of Listeria monocytogenes strains isolated in the Far East and European part of Russia on the basis of polymorphism of genes encoding invasion factors].
abstract: Forty Listeria monocytogenes isolates obtained in European and Far East regions of Russia were differentiated on the basis of polymorphism of 5 markergenes. Total length of concatemers obtained after sequencing of internal fragments of genes inlA, inlB, inlC, inlE and prs was 3029 b.p. Comparative analysis of concatemers' sequences revealed 237 variable nucleotides. Totally, 25 sequence types were revealed, and isolates from European and Far East regions belonged to different types. On the dendrogram isolates split on 2 clusters, which correspond to early described phylogenetic lines of L. monocytogenes specie. Isolates obtained in European and Far East regions formed independent subclusters within main clusters. Fifteen clinical isolates of L. monocytogenes belonged to 7 different types. Analysis of epidemiologic data on time and place of isolates obtaining suggested that isolates of the same sequence type are epidemiologically related and might represent one strain; index of discrimination for proposed typing method was calculated as 0.982.
authors.forename: E A
authors.initials: EA
authors.lastname: Zaĭtseva
authors.forename: K R
authors.initials: KR
authors.lastname: Beliaev
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Egorova
authors.forename: A I
authors.initials: AI
authors.lastname: Suniaĭkin
authors.forename: N M
authors.initials: NM
authors.lastname: Pukhovskaia
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Musatov
authors.forename: L I
authors.initials: LI
authors.lastname: Ivanov
authors.forename: D V
authors.initials: DV
authors.lastname: Kolbasov
authors.forename: G P
authors.initials: GP
authors.lastname: Somov
authors.forename: A L
authors.initials: AL
authors.lastname: Gintsburg
authors.forename: S A
authors.initials: SA
authors.lastname: Ermolaeva
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D008088:Listeriosis / Q000453:epidemiology*; D008565:Membrane Proteins / Q000235:genetics; D017720:Molecular Epidemiology; D011110:Polymorphism, Genetic; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D012800:Siberia / Q000453:epidemiology; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C455530:InlE protein, Listeria monocytogenes; D008565:Membrane Proteins; D037521:Virulence Factors; C095768:inlB protein, Listeria monocytogenes; C103178:inlC protein, Listeria monocytogenes; C069761:internalin protein, Bacteria; D012268:Ribose-Phosphate Pyrophosphokinase