Chiral separation of catechin by capillary electrophoresis using mono-, di-, tri-succinyl-beta-cyclodextrin as chiral selectors.
The chiral separation of (+/-)-catechin was investigated by capillary electrophoresis using characterized succinyl-beta-cyclodextrins (Suc-beta-CDs) with one to three degree of substitution values. The effects of nature and concentration of Suc-beta-CDs and running buffer pH on the migration time and resolution of (+/-)-catechin are discussed. All three kinds of Suc-beta-CDs show a clear baseline separation of (+/-)-catechin in capillary electrophoresis. Mono-Suc-beta-CD effectively separated (+/-)-catechin, and additional substituted CDs (di- and tri-Suc-beta-CD) were capable of chiral separation at a broad pH range. The optimum running conditions were found to be 100 mM borate buffer (pH 9.8) containing 5 mM mono-Suc-beta-CD with no methanol organic modifier.
Kim
Hyunmyung
H
Department of Bioscience and Biotechnology and Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, South Korea.
Choi
Youngjin
Y
Lim
Jun
J
Paik
Seung-R
SR
Jung
Seunho
S
D002021:Buffers; D002392:Catechin / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002021:Buffers; D047392:beta-Cyclodextrins; D002392:Catechin