Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction.
In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.
Hazra
Amrita
A
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Chatterjee
Abhishek
A
Begley
Tadhg P
TP
D001412:Bacillus subtilis / Q000737:chemistry* / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D019759:Carbon-Nitrogen Lyases / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D013831:Thiamine / Q000096:biosynthesis*; D013844:Thiazoles / Q000378:metabolism*
D001426:Bacterial Proteins; D013844:Thiazoles; D019759:Carbon-Nitrogen Lyases; C551808:thiamin thiazole synthase, Bacillus subtilis; D013831:Thiamine