title: Simultaneous determination of bioactive xanthone glycosides and norlignans from ethanolic extract of Anemarrhena asphodeloides by liquid chromatography.
abstract: The rhizomes of Anemarrhena asphodeloides Bunge (Liliaceae) are prescribed as crude drugs in herbal medication for the treatment of various diseases such as diabetes, inflammation, and platelet aggregation inhibition. A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed to study the quantitative determination of 5 bioactive compounds from these rhizomes, namely, neomangiferin, mangiferin, isomangiferin, nyasol, and methylnyasol. Chromatographic analysis was performed on Capcell Pak C18 column (150 x 4.6 mm, 3 microm) with a mobile phase consisting of acetonitrile, methanol, and 0.1% formic acid at a flow rate of 1.00 mL/min. Quantitation was performed using a UV-visible detector at 260 nm. The method for the determination of reported medicinal agents was accurate and reproducible. Excellent linear behavior was observed over the investigated concentration range of 2.5-100.0 microg/mL for neomangiferin; 1.5-60.0 microg/mL for mangiferin; 0.5-20.0 microg/mL for nyasol; and 0.2-20.0 microg/mL for methylnyasol; correlation coefficient > 0.99. The intraday and interday precision over the concentration range of compounds was < 6.6% (relative standard deviation) and accuracy was between 94.9 and 109.3%. This method can be successfully applied for the analysis of medicinal compounds from the ethanolic extract of A. asphodeloides Bunge.
authors.affiliation: Korea Institute of Science and Technology, Doping Control Center, PO Box 131, Chungryang, Seoul 130-650, Korea.
authors.forename: M Nurul
authors.initials: MN
authors.lastname: Islam
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Yoo
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
authors.forename: Joo Won
authors.initials: JW
authors.lastname: Nam
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Seo
authors.forename: Changbae
authors.initials: C
authors.lastname: Jin
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D000998:Antiviral Agents / Q000032:analysis / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006027:Glycosides / Q000032:analysis; D017705:Lignans / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D044004:Xanthones / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000998:Antiviral Agents; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; D012997:Solvents; D044004:Xanthones; C061492:isomangiferin; D000431:Ethanol