title: Mutations in genes encoding sorting nexins alter production of intracellular and extracellular proteases in Aspergillus nidulans.
abstract: XprG, a putative p53-like transcriptional activator, regulates production of extracellular proteases in response to nutrient limitation and may also have a role in programmed cell death. To identify genes that may be involved in the XprG regulatory pathway, xprG2 revertants were isolated and shown to carry mutations in genes which we have named sogA-C (suppressors of xprG). The translocation breakpoint in the sogA1 mutant was localized to a homolog of Saccharomyces cerevisiae VPS5 and mapping data indicated that sogB was tightly linked to a VPS17 homolog. Complementation of the sogA1 and sogB1 mutations and identification of nonsense mutations in the sogA2 and sogB1 alleles confirmed the identification. Vps17p and Vps5p are part of a complex involved in sorting of vacuolar proteins in yeast and regulation of cell-surface receptors in mammals. Protease zymograms indicate that mutations in sogA-C permit secretion of intracellular proteases, as in S. cerevisiae vps5 and vps17 mutants. In contrast to S. cerevisiae, the production of intracellular protease was much higher in the mutants. Analysis of serine protease gene expression suggests that an XprG-independent mechanism for regulation of extracellular protease gene expression in response to carbon starvation exists and is activated in the pseudorevertants.
authors.affiliation: Molecular and Cellular Biology, University of New England, Armidale, NSW, Australia. mkatz@une.edu.au
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Katz
authors.forename: Cara J
authors.initials: CJ
authors.lastname: Evans
authors.forename: Emma E
authors.initials: EE
authors.lastname: Heagney
authors.forename: Patricia A
authors.initials: PA
authors.lastname: vanKuyk
authors.forename: Joan M
authors.initials: JM
authors.lastname: Kelly
authors.forename: Brian F
authors.initials: BF
authors.lastname: Cheetham
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics*; D005110:Extracellular Space / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000502:physiology; D042541:Intracellular Space / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics; D008954:Models, Biological; D009154:Mutation / Q000502:physiology; D030781:Organisms, Genetically Modified; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism*; D058305:Sorting Nexins; D033921:Vesicular Transport Proteins / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D058305:Sorting Nexins; D033921:Vesicular Transport Proteins; D010447:Peptide Hydrolases; D008666:Metalloendopeptidases; C079508:PrtA metalloprotease