title: Mass spectrometric analysis using ruthenium (II)-labeling for identification of glycosyl hydrolase product.
abstract: Analysis of products digested by glycosyl hydrolases helps understanding of the hydrolysis mechanism and the substrate recognition in the enzymes. We developed a new universal technique, which consists of ruthenium (II) complex labeling and mass spectrometry analysis, to identify the reducing sugars released from oligosaccharides by enzymatic digestion. This method was applied to enzymatic digestion by chitinase and cellulase of the hyperthermophilic archaea Pyrococcus fusiosus and Pyrococcus horikoshii respectively.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ito
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Okamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Uegaki
authors.forename: Han-Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Norikazu
authors.initials: N
authors.lastname: Ueyama
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000201:enzymology; D043902:Pyrococcus horikoshii / Q000201:enzymology; D012428:Ruthenium / Q000032:analysis* / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D002241:Carbohydrates; D009942:Organometallic Compounds; D012428:Ruthenium; D002688:Chitinases; D002480:Cellulase; D009699:N-Glycosyl Hydrolases