Fourier transform infrared spectroscopy of gallbladder carcinoma cell line.
BACKGROUND
Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line.


METHODS
The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF2 window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue.


RESULTS
Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue.


CONCLUSIONS
The diagnosis of carcinoma tissue by FT-IR spectroscopy has a sufficient cellular basis.
Du
Jun-Kai
JK
Department of General Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Shi
Jing-Sen
JS
Sun
Xue-Jun
XJ
Wang
Jian-Sheng
JS
Xu
Yi-Zhuang
YZ
Wu
Jin-Guang
JG
Zhang
Yuan-Fu
YF
Weng
Shi-Fu
SF
D045744:Cell Line, Tumor; D042241:Early Diagnosis; D005704:Gallbladder / Q000473:pathology*; D005706:Gallbladder Neoplasms / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*