Stepwise synthesis of RNA conjugates carrying peptide sequences for RNA interference studies.
Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3'-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at the 3'-end of the passenger strand has similar inhibitory properties as those of unmodified or cholesterol-modified RNA duplexes.
Aviñó
Anna
A
Institute for Research in Biomedicine Barcelona, Edifici Helix, Baldiri Reixac 15, 08028 Barcelona, Spain.
Ocampo
Sandra M
SM
Caminal
Clara
C
Perales
José Carlos
JC
Eritja
Ramon
R
D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells; D006801:Humans; D019913:Nuclear Localization Signals / Q000138:chemical synthesis*; D056489:Nucleoplasmins / Q000235:genetics; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010455:Peptides / Q000138:chemical synthesis*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D019913:Nuclear Localization Signals; D056489:Nucleoplasmins; D009843:Oligoribonucleotides; D010455:Peptides; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha