[Study on bulblet induction of Fritillaria anhuiensis in vitro].
OBJECTIVE
To study the optimal condition for bulb induction of Fritillaria anhuiensis in vitro.


METHODS
Effects of sucrose, salicylic acid, active carbon and 5 degrees C pre-incubation on bulb formation in vitro were investigated by adopting the number of bulb and fresh weight as indexes.


RESULTS
The number of bulb and fresh weight in medium added with 50 g x L(-1) sucrose were significantly higher than those with other treatments. The medium with different concentrations of salicylic acid showed no significant promotion on callus differentiation. However, 5 g x L(-1) active carbon (AC) treatment was better than other treatments. Meanwhile, a large number of plantlets formed after 5 degrees C pre-incubation for 30-40 days was suitable for bulb formation and growth.


CONCLUSIONS
Bulblet formation was optimal in MS + KT 2 mg x L(-1) + NAA 2 mg x L(-1) + 50 g x L(-1) sucrose +5 g x L(-1) AC after callus pre-incucation at 5 degrees 1 for 30-40 days.
Xue
Jian-ping
JP
Anhui Key Laboratory of Plant Resources and Biology, Department of Biology, Huaibei Coal Industry Teachers' College, Anhui Province, Huaibei 235000, China. xuejp2000@yahoo.com.cn
Zhang
Ai-min
AM
Geng
Mao-lin
ML
Ma
Lin
L
D031410:Fritillaria / Q000187:drug effects / Q000254:growth & development*; D018547:Plant Stems / Q000187:drug effects* / Q000254:growth & development*; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development; D020156:Salicylic Acid / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
D013395:Sucrose; D020156:Salicylic Acid