title: Comprehensive expression analysis of all Wnt genes and their major secreted antagonists during mouse limb development and cartilage differentiation.
abstract: Wnt signalling plays important roles in patterning and outgrowth of the vertebrate limb. Different mutations in Wnt genes, their antagonists or (co-)receptors result in patterning and outgrowth defects as well as chondrocyte and bone phenotypes in mouse and human. Understanding Wnt activity during mouse limb development and chondrogenesis requires a temporal and spatial overview of Wnt signalling key factor expression. Here we present a comparative expression analysis of all 19 Wnt genes and their major secreted antagonists of the Dickkopf (Dkk), Wisp and the secreted frizzled related protein (Sfrp) families during mouse limb development. Our study reveals new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. We also identified novel expression domains for the Wnt antagonists Sfrp1, Sfrp3, Sfrp5, Wisp1 as well as Dkk2 and Dkk3. We provide a full expression pattern for Wif1 in limb development, for which no limb expression had been documented so far.
authors.affiliation: Max Planck Institute for Molecular Genetics, Ihnestr. 73, D-14195 Berlin, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Witte
authors.forename: Janine
authors.initials: J
authors.lastname: Dokas
authors.forename: Franziska
authors.initials: F
authors.lastname: Neuendorf
authors.forename: Stefan
authors.initials: S
authors.lastname: Mundlos
authors.forename: Sigmar
authors.initials: S
authors.lastname: Stricker
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D055511:CCN Intercellular Signaling Proteins; D002356:Cartilage / Q000196:embryology / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism; D004475:Ectoderm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005552:Forelimb / Q000196:embryology / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics; D008648:Mesoderm / Q000378:metabolism; D051379:Mice / Q000196:embryology / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins; D013997:Time Factors; D051153:Wnt Proteins / Q000235:genetics*; D051154:Wnt2 Protein; D000099274:Frizzled-Related Proteins; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D055511:CCN Intercellular Signaling Proteins; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; D051154:Wnt2 Protein; D000099274:Frizzled-Related Proteins; C492067:CCN4 protein, mouse; C405060:Dkk2 protein, mouse; C405061:Dkk3 protein, mouse; C485539:Sfrp1 protein, mouse; D000094742:Secreted Frizzled-Related Proteins; C487972:Sfrp5 protein, mouse; C118544:Wif1 protein, mouse