Identification of cytotoxic compound from Artocarpus communis leaves against P-388 cells.
In the course of continuing research for finding bioactive compounds from Indonesian plants, the leaves of Artocarpus communis was extracted by ethanol. This extract partitioned with n-hexane-water (1:4) and then water extract was partitioned with dichloromethane. Dichloromethane extract was purified by column chromatography techniques on silica gel to afford yellow crystal (F-1). Based on LC-MS, 1H-NMR and 13C-NMR (1D and 2D) spectra and compared with previous spectral data, it was identified as prenylated flavonoid, 1-(2,4-dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-1-benzopyran-5-yl] 1-propanone. This compound showed significant cytotoxicity against murine P-388 leukemia cells.
Lotulung
Puspa D N
PD
Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPIPTEK, Serpong, Banten 15314, Indonesia.
Fajriah
Sofa
S
Hanafi
Muhammad
M
Filaila
Euis
E
D000818:Animals; D031622:Artocarpus / Q000378:metabolism*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000379:methods; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008956:Models, Chemical; D008968:Molecular Conformation; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism*
D005419:Flavonoids; D010936:Plant Extracts; D000431:Ethanol