Nonpathogenic Entamoeba dispar quickly outgrows pathogenic Entamoeba histolytica in mixed xenic cultures.
Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic species can rapidly outgrow the pathogen in xenic cultures.
Pysova
I
I
Department of Tropical Medicine, Teaching Hospital Bulovka, Prague, Czech Republic.
Tumova
P
P
Tolarova
V
V
Nohynkova
E
E
D000818:Animals; D018920:Coculture Techniques; D003470:Culture Media; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D004747:Entamoeba / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000175:diagnosis / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity
D003470:Culture Media; D016054:DNA, Protozoan