title: Expression of Histamine HReceptors in Xenopus Oocytes Injected with Messenger Ribonucleic Acid from Bovine Adrenal Medulla: Pertussis Toxin Insensitive Activation of Membrane Chloride Currents.
abstract: Abstract Histamine H(2)-receptors have been identified in Xenopus oocytes previously microinjected with poly(A) + ribonucleic acid from bovine adrenal glands. Bath application of histamine to ribonucleic acid-primed oocytes evoked concentration-dependent, oscillating membrane currents under voltage-clamp conditions. H(1)-receptor specific antagonists clemastine, doxepin, pyrilamine, promethacine, diphenylhydramine, dephenylpyraline and chlorpheniramine, but not H(2)-receptor antagonists, cimetidine and ranitidine, inhibited histamine-induced responses. Membrane currents evoked by bath-applied histamine were insensitive to pertussis toxin, carried by chloride ions and dependent on intracellular but not extracellular calcium.
authors.affiliation: Institut für Zellbiochemie und klinische Neurobiologie, Universität Hamburg, UKE, Martinistr. 52, D-2000 Hamburg 20, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Meyerhof
authors.forename: J R
authors.initials: JR
authors.lastname: Schwärzt
authors.forename: V
authors.initials: V
authors.lastname: Höllt
authors.forename: D
authors.initials: D
authors.lastname: Richter