title: Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.
abstract: Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype have been identified as a potential pandemic threat by the World Health Organization (WHO). Since 1997, these viruses have been spreading from Asia to Europe and Africa with increasing genetic and antigenic diversities. Vaccination is the preferred strategy for the prevention and control of influenza infections and the availability of a system for the rapid engineering and production of vaccines is required in the event of an influenza pandemic. In this study, we engineered and produced recombinant hemagglutinin (HA) from A/Bar-headed Goose/Qinghai/1A/05 (clade 2.2) and A/Anhui/1/2005 (clade 2.3) in Nicotiana benthamiana plants. Immunization of mice with these plant-derived HA antigens elicited serum hemagglutination inhibition (HI) and virus neutralization (VN) antibodies. These results suggest the utility of our plant-expression system for recombinant influenza vaccine production.
authors.affiliation: Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE 19711, USA.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shoji
authors.forename: Christine E
authors.initials: CE
authors.lastname: Farrance
authors.forename: Hong
authors.initials: H
authors.lastname: Bi
authors.forename: Moneim
authors.initials: M
authors.lastname: Shamloul
authors.forename: Brian
authors.initials: B
authors.lastname: Green
authors.forename: Slobodanka
authors.initials: S
authors.lastname: Manceva
authors.forename: Amy
authors.initials: A
authors.lastname: Rhee
authors.forename: Natalia
authors.initials: N
authors.lastname: Ugulava
authors.forename: Gourgopal
authors.initials: G
authors.lastname: Roy
authors.forename: Konstantin
authors.initials: K
authors.lastname: Musiychuk
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Chichester
authors.forename: Vadim
authors.initials: V
authors.lastname: Mett
authors.forename: Vidadi
authors.initials: V
authors.lastname: Yusibov
mesh_terms: D000818:Animals; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014026:Nicotiana / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic