The influence of developmental stages and protective additives on cryopreservation of surf clam (Spisula sachalinensis) larvae.
This study was performed to find out the optimal larval stage and the most desirable protective additives for cryopreservation of surf clam, Spisula sachalinensis larvae. The survival rates of frozen-thawed larvae increased with post developmental stage. The highest value of 96.1 +/- 1.0% was achieved using umbo stage larva as developmental stage and 0.2 M sucrose as protective additive.
Choi
Youn Hee
YH
Institute of Fisheries Sciences, Pukyong National University, Nam-gu, Busan 608-737, Korea.
Lee
Jeong Yong
JY
Chang
Young Jin
YJ
D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004187:Disaccharides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D013696:Temperature
D003451:Cryoprotective Agents; D004187:Disaccharides; D005632:Fructose; D005947:Glucose