Assessment of serum paraoxonase and arylesterase activities in early pregnancy failure.
OBJECTIVE
Paraoxonase-1 (PON1) is an HDL-bound enzyme system which plays a key role in the protection of LDL and HDL from oxidation by hydrolysing activated phospholipids and lipid peroxide products. Oxidative stress has been involved in the pathogenesis of many complications of human pregnancy, including early pregnancy failure (EPF), preeclampsia and preterm labour. The purpose of this study was to determine serum paraoxonase/arylesterase activities and lipid hydroperoxide (LOOH) levels as an oxidative stress indicator in women with EPF.


METHODS
Paraoxonase/arylesterase activities and LOOH levels were assessed in women with EPF (n = 40) and healthy continuum pregnant women without EPF (n = 38) before 12 weeks' gestation. Serum basal and salt-stimulated paraoxonase/arylesterase activities were measured spectrophotometrically. LOOH levels were measured by ferrous oxidation with a xylenol orange assay. Student's t-test and the Pearson correlation were used for statistical analysis.


RESULTS
We found that basal and salt-stimulated paraoxonase/arylesterase activities were significantly lower in women with EPF than in women without EPF (all p <0.05), while LOOH levels were significantly higher (p = 0.009).


CONCLUSIONS
Our study showed that decreased paraoxonase/arylesterase activities and increased LOOH levels may play a role in ethiopathogenesis through increased susceptibility to lipid peroxidation in women with EPF.
Toy
H
H
Department of Obstetrics & Gynecology, School of Medicine, Harran University, Sanliurfa, Turkey. haruntoy@hotmail.com
Camuzcuoglu
H
H
Celik
H
H
Erel
O
O
Aksoy
N
N
D000022:Abortion, Spontaneous / Q000097:blood* / Q000503:physiopathology; D043303:Aryldialkylphosphatase / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008054:Lipid Peroxides / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy
D008054:Lipid Peroxides; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human