Identification of Vibrio anguillarum outer membrane vesicles related to immunostimulation in the Japanese flounder, Paralichthys olivaceus.
We identified outer membrane vesicle (OMV) production in Vibrio anguillarum O1, a major fish pathogen that causes vibriosis, and characterized the OMVs. They were produced during normal growth, and were appeared as spherical vesicle fractions. The protein profile of the OMVs was similar to that of the outer membrane proteins, and the 38-kDa major protein band of OMV was identified as OmpU. The OMVs had enzyme activity of metalloprotease, hemolysin, and phospholipase, and stimulated the production of proinflammatory cytokines, such as TNF-alpha, IL-1beta, and IL-6 when injected into the flounder.
Hong
Gyeong-Eun
GE
Department of Biotechnology, Pukyong National University, Busan, Korea.
Kim
Dong-Gyun
DG
Park
Eun-Mi
EM
Nam
Bo-Hye
BH
Kim
Young-Ok
YO
Kong
In-Soo
IS
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology* / Q000648:ultrastructure; D016207:Cytokines / Q000378:metabolism; D005412:Flatfishes / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007114:Immunization; D051379:Mice; D046529:Microscopy, Electron, Transmission; D014733:Vibrio / Q000166:cytology* / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines