Osmium complexation of mismatched DNA: effect of the bases adjacent to mismatched 5-methylcytosine.
The efficiency of osmium complex formation at 5-methylcytosine in mismatched DNA duplexes is a key point for the design of sequence-specific detection of DNA methylation. Osmium complexation was not observed in fully matched duplexes, whereas the complexation site and efficiency in mismatched duplexes changed depending on the type of 5'-neighboring base of the 5-methylcytosine forming a mismatched base pair. In particular, when the base adjacent to the 5' side of the mismatched base pair was thymine, a unique "side reaction" was observed. However, the nature of the mismatched base pairs in the reaction site did not influence the selectivity of osmium complex formation with methylated DNA.
Nomura
Akiko
A
Advanced Science Institute, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.
Tainaka
Kazuki
K
Okamoto
Akimitsu
A
D044503:5-Methylcytosine / Q000737:chemistry*; D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction
D009992:Osmium; D044503:5-Methylcytosine; D004247:DNA