title: Neutral pH hydrogen-enriched electrolyzed water achieves tumor-preferential clonal growth inhibition over normal cells and tumor invasion inhibition concurrently with intracellular oxidant repression.
abstract: The properties and effects of neutral pH hydrogen-enriched electrolyzed water (NHE water) on tumor cells were examined. NHE water diminished hydroxyl radicals as demonstrated by ESR in a cell-free system. Human tongue carcinoma cells HSC-4 were inhibited for either colony formation efficiencies or colony sizes by NHE water without significant inhibition to normal human tongue epithelial-like cells DOK. Furthermore, NHE water caused growth inhibition, cell degeneration, and inhibition of invasion through the reconstituted basement membrane to human fibrosarcoma cells HT-1080. Intracellular oxidants such as hydroperoxides and hydrogen peroxides were scavenged in HSC-4 or HT-1080 cells by NHE water. In the human oral cavity, a dissolved hydrogen concentrations (DH) of NHE water was drastically declined from 1.1 to 0.5 ppm, but settled to 0.3-0.4 ppm until 180 s, upon static holding without gargling. Thus, NHE water was shown to achieve tumor-preferential growth inhibition and tumor invasion together with scavenging of intracellular oxidants, and is expected as a preventive material against tumor progression and invasion.
authors.affiliation: Cell-Death Control BioTechnology Laboratory, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Hiroshima 727-0023, Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Saitoh
authors.forename: Hajime
authors.initials: H
authors.lastname: Okayasu
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Harata
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Miwa
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004572:Electrolysis; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D016877:Oxidants / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D014059:Tongue / Q000166:cytology; D014867:Water / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D016877:Oxidants; D017382:Reactive Oxygen Species; D014867:Water; D017665:Hydroxyl Radical; D006859:Hydrogen