The effect of laminin peptide gradient in enzymatically cross-linked collagen scaffolds on neurite growth.
Guided neurite growth is critical in both peripheral nervous system and central nervous system nerve regeneration. Scaffolds that provide structural and guidance cues for neuronal cells have a potential role in neural regeneration application. Type I collagen is suitable to be processed as an engineered scaffold for nerve regeneration because of its biological and structural properties. A few previous studies have shown that cross-linking of collagen scaffolds with microbial transglutaminase improves the mechanical strength and degradation properties of the scaffolds. It was shown that laminin 5 can regulate neurite outgrowth and extension. A motif (PPFLMLLKGSTR) in the human laminin 5 alpha 3 chain is crucial for both integrin alpha 3 beta 1 receptor binding and cell adhesion. In the present work, we studied the guidance effect of a laminin peptide (PPFLMLLKGSTR) gradient in collagen and cross-linked collagen scaffolds on neurite growth. Neurites of rat pheochromocytoma (PC12) cells showed a preferential growth toward the high laminin concentration level on the collagen scaffold, while the incorporation of laminin peptide in the scaffold did not influence neurite length of PC12 cells.
Yao
Li
L
National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
Damodaran
Gopinath
G
Nikolskaya
Natalia
N
Gorman
Adrienne M
AM
Windebank
Anthony
A
Pandit
Abhay
A
D000818:Animals; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007797:Laminin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009416:Nerve Regeneration; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D013306:Streptomycetaceae / Q000201:enzymology; D054457:Tissue Scaffolds / Q000737:chemistry*; D011503:Transglutaminases / Q000737:chemistry
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D007797:Laminin; D003094:Collagen; D011503:Transglutaminases; D016650:Fluorescein-5-isothiocyanate