title: Facile evaluation of cell disruption efficiency using pH-controlled fluorescence resonance energy transfer.
abstract: The evaluation of cell disruption efficiency is important in monitoring durability and diverse cellular physiologies of host cells and improving recovery yield of intracellular products. On the basis of our previously suggested pH-controlled fluorescence resonance energy transfer (FRET) system, we herein demonstrated that easy and effective estimation of the degree of cell disruption under certain stress environments is possible using a simple measurement of changes in fluorescence spectra of two fused fluorescent proteins, because its unique linear correlation was established with cell disruption efficiency from the conventional colony counting method.
authors.affiliation: Dept of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects; D031541:Fluorescence Resonance Energy Transfer; D006863:Hydrogen-Ion Concentration; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D012967:Sodium Dodecyl Sulfate