title: Analysis of neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry.
abstract: A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression and localization of neuropeptides in the adult D. melanogaster brain. In extract from the whole adult brain, 42 neuropeptides from 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations of cells which were enriched with immunoaffinity purification using a genetically expressed marker.
authors.affiliation: Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA. joanne_yew@hms.harvard.edu
authors.forename: Joanne Y
authors.initials: JY
authors.lastname: Yew
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Natasha
authors.initials: N
authors.lastname: Barteneva
authors.forename: Sergei
authors.initials: S
authors.lastname: Dikler
authors.forename: Kimberly K
authors.initials: KK
authors.lastname: Kutz-Naber
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
authors.forename: Edward A
authors.initials: EA
authors.lastname: Kravitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002490:Central Nervous System / Q000378:metabolism / Q000648:ultrastructure; D004331:Drosophila melanogaster / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009479:Neuropeptides