title: The influence of developmental stages and protective additives on cryopreservation of surf clam (Spisula sachalinensis) larvae.
abstract: This study was performed to find out the optimal larval stage and the most desirable protective additives for cryopreservation of surf clam, Spisula sachalinensis larvae. The survival rates of frozen-thawed larvae increased with post developmental stage. The highest value of 96.1 +/- 1.0% was achieved using umbo stage larva as developmental stage and 0.2 M sucrose as protective additive.
authors.affiliation: Institute of Fisheries Sciences, Pukyong National University, Nam-gu, Busan 608-737, Korea.
authors.forename: Youn Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Jeong Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004187:Disaccharides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D013696:Temperature
chemical_list: D003451:Cryoprotective Agents; D004187:Disaccharides; D005632:Fructose; D005947:Glucose