Detection of differential proteomes of human beta-cells during islet-like differentiation using iTRAQ labeling.
A human beta-cell line, RNAKT-15, was recently established from human pancreatic islets, whereby its differentiation into islet-like beta-cells (islet-like RNAKT-15) increased its expression of insulin 2-fold compared with RNAKT-15 cells. To characterize the differentiation of RNAKT-15 cells into islet-like RNAKT-15, microarray and quantitative proteomics were performed. Our analysis of differential proteomic and mRNA expression has resulted in a greater understanding of the molecular functions that are involved in beta-cell differentiation and insulin synthesis and release.
Jin
Jonghwa
J
Departments of Biomedical Sciences and Internal Medicine, Genome Research Center for Diabetes and Endocrine Disease, Seoul National University College of Medicine, 28 Yongon-Dong, Seoul 110-799, Korea.
Park
Jungeun
J
Kim
Kyunggon
K
Kang
Yup
Y
Park
Sang Gyu
SG
Kim
Jae Hyeon
JH
Park
Kyong Soo
KS
Jun
Heesook
H
Kim
Youngsoo
Y
D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002853:Chromatography, Liquid; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000502:physiology*; D007553:Isotope Labeling; D020543:Proteome / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D007328:Insulin; D020543:Proteome; D012333:RNA, Messenger