Hydrogen production by immobilized R. faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria E. harbinense B49.
In order to increase the hydrogen yield from glucose, hydrogen production by immobilized Rhodopseudomonas faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria Ethanoligenens harbinense B49 was investigated. The soluble metabolites from dark-fermentation mainly were ethanol and acetate, which could be further utilized for photo-hydrogen production. Hydrogen production by B49 was noticeably affected by the glucose and phosphate buffer concentration. The maximum hydrogen yield (1.83 mol H(2)/mol glucose) was obtained at 9 g/l glucose. In addition, we found that the ratio of acetate/ethanol (A/E) increased with increasing phosphate buffer concentration, which is favorable to further photo-hydrogen production. The total hydrogen yield during dark- and photo-fermentation reached its maximum value (6.32 mol H(2)/mol glucose) using 9 g/l glucose, 30 mmol/l phosphate buffers and immobilized R. faecalis RLD-53. Results demonstrated that the combination of dark- and photo- fermentation was an effective and efficient process to improve hydrogen yield from a single substrate.
Liu
Bing-Feng
BF
State Key Lab of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, No. 202 Haihe Road, Harbin, China.
Ren
Nan-Qi
NQ
Xing
De-Feng
DF
Ding
Jie
J
Zheng
Guo-Xiang
GX
Guo
Wan-Qian
WQ
Xu
Ji-Fei
JF
Xie
Guo-Jun
GJ
D000085:Acetates / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000378:metabolism*; D012995:Solubility
D000085:Acetates; D010710:Phosphates; D000431:Ethanol; D006859:Hydrogen; D005947:Glucose