Mitochondrial DNA analysis of Sporothrix schenckii in India, Thailand, Brazil, Colombia, Guatemala and Mexico.
Up to now, 30 mitochondrial DNA(mtDNA)and 4 rDNA types of Sporothrix schenckii strains have been identified. Here, seventy-six isolates of S. schenckii from Mexico, Guatemala, Brazil, Thailand and India were genotyped and studied epidemiologically by mtDNA restriction fragment length polymorphisms(RFLP)and internal transcribed spacer region(ITS)-RFLP analysis and two new mtDNA types, Type 31 and Type 32, were found. Type 30, previously reported by Mora-Cabrera et al. was confirmed to be Type 3 and designated as blank. Of 48 isolates from Mexico, 41 belonged to Group A wherein Type 2(13 isolates), Type 3(10)and Type 28(7)were dominant. All ten isolates from India and Thailand belonged to Group B. The 52 Group A and 24 Group B isolates corresponded to rDNA Type I and Type IV , respectively, reported by Watanabe et al.(Nippon Ishinkin Gakkai Zasshi 45: 165-175, 2004).
Ishizaki
Hiroshi
H
Division of Dermatomycology (Novartis Pharma), Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Kawasaki
Masako
M
Anzawa
Kazushi
K
Mochizuki
Takashi
T
Chakrabarti
Arunaloke
A
Ungpakorn
Rataporn
R
Torres Guererro
Haydee
H
Toriello
Conchita
C
Arenas
Robert
R
D001938:Brazil; D003105:Colombia; D004271:DNA, Fungal / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D006166:Guatemala; D007194:India; D008800:Mexico; D012150:Polymorphism, Restriction Fragment Length; D013175:Sporothrix / Q000145:classification / Q000235:genetics*; D013785:Thailand
D004271:DNA, Fungal; D004272:DNA, Mitochondrial