title: Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus.
abstract: Three different dissolved oxygen (DO) control approaches were proposed to improve hyaluronic acid (HA) production: a three-stage agitation speed control approach, a two-stage DO control approach, and an oxygen vector perfluorodecalin (PFC) applied approach. In the three-stage agitation speed control approach, agitation speed was 200 rpm during 0-8 h, 400 rpm during 8-12 h, and 600 rpm during 12-20 h. In the two-stage DO control strategy, DO was controlled at above 10% during 0-8 h and at 5% during 8-20 h. In the PFC applied approach, PFC (3% v/v) was added at 8 h. HA production reached 5.5 g/L in the three-stage agitation speed control culture model, and 6.3 g/L in two-stage DO control culture model, and 6.6 g/L in the PFC applied culture model. Compared with the other two DO control approaches, the PFC applied approach had a lower shear stress and thus a higher HA production was achieved.
authors.affiliation: School of Biotechnology, Jiangnan University, 214122 Wuxi, China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D003470:Culture Media; D005285:Fermentation; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007700:Kinetics; D010101:Oxygen Consumption; D012212:Rheology; D018502:Streptococcus equi / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006820:Hyaluronic Acid