title: Intracellular adaptation of Brucella abortus.
abstract: Macrophages were infected with virulent Brucella abortus strain 2308 or attenuated strain 19. Intracellular bacteria were recovered at different times after infection and their proteomes compared. The virulent strain initially reduced most biosynthesis and altered its respiration; adaptations reversed later in infection. The attenuated strain was unable to match the magnitude of the virulent strain's adjustments. The results provide insight into mechanisms utilized by Brucella to establish intracellular infections.
authors.affiliation: Caprion Proteomics, Inc., 7150 Alexander-Fleming, Montreal, Quebec, Canada, H4S 2C8.
authors.forename: Julie
authors.initials: J
authors.lastname: Lamontagne
authors.forename: Anik
authors.initials: A
authors.lastname: Forest
authors.forename: Elena
authors.initials: E
authors.lastname: Marazzo
authors.forename: François
authors.initials: F
authors.lastname: Denis
authors.forename: Heather
authors.initials: H
authors.lastname: Butler
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Michaud
authors.forename: Lyne
authors.initials: L
authors.lastname: Boucher
authors.forename: Ida
authors.initials: I
authors.lastname: Pedro
authors.forename: Annie
authors.initials: A
authors.lastname: Villeneuve
authors.forename: Dmitri
authors.initials: D
authors.lastname: Sitnikov
authors.forename: Karine
authors.initials: K
authors.lastname: Trudel
authors.forename: Najib
authors.initials: N
authors.lastname: Nassif
authors.forename: Djamila
authors.initials: D
authors.lastname: Boudjelti
authors.forename: Fadi
authors.initials: F
authors.lastname: Tomaki
authors.forename: Esteban
authors.initials: E
authors.lastname: Chaves-Olarte
authors.forename: Caterina
authors.initials: C
authors.lastname: Guzmán-Verri
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brunet
authors.forename: Alexandra
authors.initials: A
authors.lastname: Côté-Martin
authors.forename: Joanna
authors.initials: J
authors.lastname: Hunter
authors.forename: Edgardo
authors.initials: E
authors.lastname: Moreno
authors.forename: Eustache
authors.initials: E
authors.lastname: Paramithiotis
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002003:Brucella abortus / Q000378:metabolism*; D002460:Cell Line; D002853:Chromatography, Liquid; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome