Whole genome sequence analysis of Mycobacterium bovis bacillus Calmette-Guérin (BCG) Tokyo 172: a comparative study of BCG vaccine substrains.
To investigate the molecular characteristics of bacillus Calmette-Guérin (BCG) vaccines, the complete genomic sequence of Mycobacterium bovis BCG Tokyo 172 was determined, and the results were compared with those for BCG Pasteur and other M. tuberculosis complex. The genome of BCG Tokyo had a length of 4,371,711bp and contained 4033 genes, including 3950 genes coding for proteins (CDS). There were 18 regions of difference (showing differences of more than 20bp), 20 insertion or deletion (ins/del) mutations of less than 20bp, and 68 SNPs between the two BCG substrains. These findings are useful for better understanding of the genetic differences in BCG substrains due to in vitro evolution of BCG.
Seki
Masaaki
M
Japan BCG Laboratory, Research of Product, 3-1-5 Matsuyama, Kiyose City, Tokyo 204-0022, Japan. seki@bcg.gr.jp
Honda
Ikuro
I
Fujita
Isao
I
Yano
Ikuya
I
Yamamoto
Saburo
S
Koyama
Akira
A
D001500:BCG Vaccine / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity
D001500:BCG Vaccine; D004269:DNA, Bacterial