title: Rrp2, a sigma54-dependent transcriptional activator of Borrelia burgdorferi, activates rpoS in an enhancer-independent manner.
abstract: Rrp2 is the sole sigma(54)-dependent transcriptional activator present in the Borrelia burgdorferi genome. We showed that recombinant Rrp2 binds to DNA in a sequence-nonspecific manner. During infection, Rrp2 activates sigma(54)-dependent rpoS expression without an apparent upstream enhancer element commonly associated with other sigma(54)-dependent transcriptional activators.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Jon S
authors.initials: JS
authors.lastname: Blevins
authors.forename: Haijun
authors.initials: H
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: He
authors.forename: Michael V
authors.initials: MV
authors.lastname: Norgard
authors.forename: Larry
authors.initials: L
authors.lastname: Reitzer
authors.forename: X Frank
authors.initials: XF
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D025065:Borrelia burgdorferi / Q000502:physiology*; D004269:DNA, Bacterial / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051877:RNA Polymerase Sigma 54 / Q000378:metabolism; D012808:Sigma Factor / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; D015534:Trans-Activators; C069567:sigma factor KatF protein, Bacteria; D051877:RNA Polymerase Sigma 54