Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.
Alanine racemase (DadX(OF4)), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P2(1)2(1)2(1), with two protein molecules in the asymmetric unit.
Ju
Jiansong
J
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Qi
Jianxun
J
Xu
Shujing
S
Ohnishi
Kouhei
K
Benedik
Michael J
MJ
Xue
Yanfen
Y
Ma
Yanhe
Y
D000411:Alanine Racemase / Q000737:chemistry* / Q000302:isolation & purification; D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration
D000411:Alanine Racemase