Hydrophilic interaction liquid chromatography with alcohol as a weak eluent.
There has been a significant increase of interest in polar compound separation by hydrophilic interaction liquid chromatography (HILIC), in which acetonitrile is mostly used as a weak eluent. Although replacing acetonitrile with alcohols as organic modifiers has been previously reported, the separation mechanism was poorly understood. In this paper we explored the separation mechanism through the method development for the analysis of the trace amounts of polar and basic hydrazines, which were genotoxic in nature. Separation parameters such as the type and concentration of alcohol, acid modifier, and buffer in mobile phase as well as the choice of stationary phase and column temperature were studied. The data indicated that both electrostatic and hydrophilic interactions contributed to the retention and separation of the hydrazines. The results presented here provide insight into the adjustment of the retention and separation of analytes in HILIC mode with alcohol as a weak eluent. The optimized HILIC method coupled with chemiluminescent nitrogen detection (CLND) is simple and sensitive (reporting limit at 0.02%) and was applied to simultaneous analysis of hydrazine and 1,1-dimethylhydrazine in a pharmaceutical intermediate.
Liu
Min
M
Analytical Research & Development, Amgen Inc, Thousand Oaks, CA 91320, USA. mliu@amgen.com
Ostovic
Judy
J
Chen
Emily X
EX
Cauchon
Nina
N
D000144:Acids, Acyclic / Q000737:chemistry; D000438:Alcohols / Q000737:chemistry*; D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D006834:Hydrazines / Q000032:analysis / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008163:Luminescent Measurements; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D013696:Temperature
D000144:Acids, Acyclic; D000438:Alcohols; D002021:Buffers; D006834:Hydrazines