title: [Isolation, differentiation and biochemical aspects of amniotic fluid stem cell].
abstract: The mesenchymals stem cells (MSCs) are cells with the great potential of differentiation are being introduced in the clinic for treatment of several diseases. Mesenchymal stem cells have several advantages including the stability of their phenotype in vitro.
  
  
  BACKGROUND
  isolation of MSCs in amniotic fluid, its expansion and the demonstration of the capacity of these cells to differentiate in adipogenic and myogenic cells, without to change the chromosomal stability of the MSCs in culture.
  
  
  METHODS
  in order to evaluate the functional change of these cells, were gotten values of the differentiated adipogenic cells and not differentiated through the dosage of triglycerides. The myogenic nature of the differentiated cells was analyzed comparing the creatine kinase--CK, lactic dehydrogenase--LDH and aldolase produced by the cells.
  
  
  RESULTS
  the values of triglycerides were significantly higher in differentiated cells, showing intracytoplasmatic granule form after differentiation. All the biochemical characters were significantly higher in differentiated myogenic cells.
  
  
  CONCLUSIONS
  this study suggests that the standardized protocol of differentiation can be used in the attainment of cells with characteristics of adipogenic and muscular cells, from amniotic fluid.
authors.affiliation: Departamento de Genética Humana e pela Criovida do Instituto H. Pardini, Hospital das Clínicas, Faculdade de Farmácia da Universidade Federal de Minais Gerais, Belo Horizonte, MG.
authors.forename: Antônio Carlos Vieira
authors.initials: AC
authors.lastname: Cabral
authors.forename: Patrícia Caroline
authors.initials: PC
authors.lastname: Angelo
authors.forename: Henrique Vitor
authors.initials: HV
authors.lastname: Leite
authors.forename: Alamanda Kfouri
authors.initials: AK
authors.lastname: Pereira
authors.forename: Ana Paula Brum Miranda
authors.initials: AP
authors.lastname: Lopes
authors.forename: Maria Beatriz de
authors.initials: MB
authors.lastname: Oliveira
authors.forename: Karina Braga Gomes
authors.initials: KB
authors.lastname: Borges
authors.forename: Victor Cavalcanti
authors.initials: VC
authors.lastname: Pardini
authors.forename: Alessandro Clayton de Sousa
authors.initials: AC
authors.lastname: Ferreira
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology* / Q000201:enzymology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000032:analysis*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011247:Pregnancy; D014280:Triglycerides / Q000097:blood
chemical_list: D014280:Triglycerides; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005634:Fructose-Bisphosphate Aldolase