title: Fourier transform infrared spectroscopy of gallbladder carcinoma cell line.
abstract: BACKGROUND
  Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line.
  
  
  METHODS
  The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF2 window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue.
  
  
  RESULTS
  Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue.
  
  
  CONCLUSIONS
  The diagnosis of carcinoma tissue by FT-IR spectroscopy has a sufficient cellular basis.
authors.affiliation: Department of General Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Du
authors.forename: Jing-Sen
authors.initials: JS
authors.lastname: Shi
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Sun
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Wang
authors.forename: Yi-Zhuang
authors.initials: YZ
authors.lastname: Xu
authors.forename: Jin-Guang
authors.initials: JG
authors.lastname: Wu
authors.forename: Yuan-Fu
authors.initials: YF
authors.lastname: Zhang
authors.forename: Shi-Fu
authors.initials: SF
authors.lastname: Weng
mesh_terms: D045744:Cell Line, Tumor; D042241:Early Diagnosis; D005704:Gallbladder / Q000473:pathology*; D005706:Gallbladder Neoplasms / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*