[Comparison of induction L-form of Staphylococcus epidermidis, Staphylococcus haemolyticus by cefazolin].
The L-forms of bacteria have not been studied carefully yet, because it is difficult to detect them in Gram stain reactions by light microscopy. They can be cultured on specialized hypertonic medium. We don't find any reports about intentional in vitro induction and assessment of frequency of L-forms of S. epidermidis and S. haemolyticus on the medium.


OBJECTIVE
to evaluate the frequency of induction of L-forms by Coagulase Negative Staphylococci. This thesis examines, if the source of isolation from clinical materials has an influence on the frequency of occurrence of cell-wall deficient bacteria.


METHODS
52 strains of S. epidermidis, 52 strains of S. haemolyticus were analysed. After 13 S. epidermidis and S. haemolyticus strains were isolated from blood, urine, biomaterials, changed surface skin from patients of University Hospital in Bydgoszcz. S. epidermidis and S. haemolyticus strains were tested for induction of L-forms the methods of Owens (1988).


RESULTS
It was observed that four (7.7%) strains of and S. haemolyticus transformed into L-forms. S. epidermidis strains isolated from blood induced L-forms (two strains), from urine and biomaterial (one strain). It was observed that strains of S. haemolyticus which have been isolated from blood and urine induced L-forms (three strains and one respectively).


CONCLUSIONS
This study suggest that L-form induction in S. epidermidis and of S. haemolyticus strains is not correlated with sample origin from which the strains had been isolated. S. epidermidis and S. haemolyticus strains produce L-forms rarely.
Wróblewska
Joanna
J
Uniwersytet Mikołaja Kopernika w Toruniu, Collegium Medicum w Bydgoszczy, Katedra i Zakład Mikrobiologii. joannawroblewska@wp.pl
Gospodarek
Eugenia
E
Sekowska
Alicja
A
Mikołajczyk
Dorota
D
Janicka
Grazyna
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001769:Blood / Q000382:microbiology; D002437:Cefazolin / Q000494:pharmacology*; D006801:Humans; D012867:Skin / Q000382:microbiology; D013045:Species Specificity; D013212:Staphylococcus epidermidis / Q000145:classification* / Q000187:drug effects*; D041162:Staphylococcus haemolyticus / Q000145:classification* / Q000187:drug effects*; D014556:Urine / Q000382:microbiology
D000900:Anti-Bacterial Agents; D002437:Cefazolin