Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization.
We discovered a novel method to prepare a protein-based hydrogel, that is, a "Three-Dimensional Nanostructured Protein Hydrogel (3D NPH)", which is composed of protein-polymer hybrid nanoparticles. In this study, we propose a novel protein microarray whose 3D NPH spots were prepared by dispensing a small volume of the solution of protein-polymer mixture on a substrate. The dispensed solution had a short time for cross-linking before its drying-up and the resulting 3D NPH had loosely cross-linked, thin spongy structure. Therefore, the reaction ratio between ligands and analytes was drastically improved in this system compared with the large volume system for Surface Plasmon Resonance (SPR) protein microarray.
Tanaka
Hiroyuki
H
Graduate School of Science and Technology, Keio University, Yokohama, Japan.
Hanasaki
Minako
M
Isojima
Tatsushi
T
Takeuchi
Hisao
H
Shiroya
Toshifumi
T
Kawaguchi
Haruma
H
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D021621:Imaging, Three-Dimensional / Q000379:methods*; D055767:Immobilized Proteins / Q000737:chemistry*; D008024:Ligands; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance
D003432:Cross-Linking Reagents; D020100:Hydrogels; D055767:Immobilized Proteins; D008024:Ligands; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D019809:Streptavidin