Determination of flumorph residues in vegetables, soil, and natural water by solid-phase extraction cleanup and high-performance liquid chromatography with UV detection.
A method for high-performance liquid chromatographic (HPLC) determination of flumorph residues in cucumber, tomato, soil, and natural water was developed and validated. Primary secondary amine or octadecylsilyl (C18) solid-phase extraction cartridges were used for sample preparation. Reversed-phase HPLC with UV detection was used for separation and quantification of the pesticide. The combined cleanup and chromatographic method steps were sensitive and reliable for simultaneous determination of residues of the 2 isomers of flumorph in the studied samples. This method is characterized by recovery > 97.9%, coefficient of variation < 6.2%, and limit of quantification of 0.01 mg/kg, in agreement with directives for method validation in residue analysis. Flumorph residues in the samples were further confirmed by HPLC/mass spectrometry. The proposed method is fast, easy to perform, and could be used for monitoring of pesticide residues.
Hu
Ji-Ye
JY
University of Science and Technology Beijing, School of Applied Science, Beijing 100083, People's Republic of China. jiyehu@sas.ustb.edu.cn
Zhang
Yu-Chao
YC
Yan
Hai
H
D002851:Chromatography, High Pressure Liquid; D005659:Fungicides, Industrial / Q000032:analysis*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009025:Morpholines / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D012989:Soil Pollutants / Q000032:analysis*; D052616:Solid Phase Extraction; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014675:Vegetables / Q000737:chemistry*; D014873:Water Pollutants / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
D005659:Fungicides, Industrial; D007202:Indicators and Reagents; D009025:Morpholines; D010573:Pesticide Residues; D012989:Soil Pollutants; D012996:Solutions; D014873:Water Pollutants; C528737:flumorph