Evaluation of a digoxigenin-labelled probe for detection of Aeromonas spp.
An outer membrane protein-based Digoxigenin (DIG)-labelled DNA probe was developed for the specific detection of Aeromonas sp. from food/environmental/clinical samples. Dot blot reaction answered for all the Aeromonas isolates and was negative for Escherichia coli, Pseudomonas sp., Klebsiella sp., Vibrio parahaemolyticus, V. harveyi, V. alginolyticus, V. vulnificus. Edwardsiella tarda and Staphylococcus sp. As this protein is highly conserved in various Aeromonas species, the probe has the potential for use as a rapid and reliable diagnostic tool.
Khushiramani
R
R
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore, India.
Girisha
S K
SK
Karunasagar
I
I
Karunasagar
I
I
D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D000818:Animals; D015373:Bacterial Typing Techniques; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D004076:Digoxigenin; D006801:Humans; D012680:Sensitivity and Specificity; D014735:Vibrio Infections / Q000175:diagnosis / Q000382:microbiology
D015342:DNA Probes; D004269:DNA, Bacterial; D004076:Digoxigenin