Polarization from a helix of fluorophores and its relation to that obtained from muscle.
Expressions for the polarization of fluorescence from fluorophores in a helical array have been obtained. These predictions have been compared with experimental data obtained by reacting fluorescent S-1 subfragments of myosin (extrinsically labeled with N-(iodoacetylaminoethyl)-5-napthylamine-1-sulfonic acid [1,5-IAEDANS]) with glycerated rabbit psoas fibers in rigor. A comparison with data obtained by directly labeling fibers with this fluorophore is also presented.
Tregear
R T
RT
University of California, San Francisco, California 94143, USA.
Mendelson
R A
RA