[Modification of bacterial population structure on anti-lysozyme activity under the influence of hexylresorcinol].
OBJECTIVE
To investigate the modifying effect of hexylresorcinol chemical analog on anti-lysozyme activity of bacteria.


METHODS
Influence of hexylresorcinol on anti-lysozyme activity (ALA) of bacteriawas studied on the examples of such strains as Bacillus cereus 279, Klebsiella pneumoniae 278, Escherichia coli 101, which are able to inactivate lysozyme. As a chemical analog of microbial regulators, C6-alkyloxybenzol (C6-AOB) was used, which was introduced in growth medium containing microorganisms, which than were cultivated until entering in the stationary growth phase. Isolation of clones was carried out by seeding the 24-hours broth culture on solid growth medium followed by measurement of ALA with photometric method.


RESULTS
Modifying effect of chemical analog of extracellular microbial regulators (C6-AOB) on population structure of bacteria on the sign of anti-lysozyme activity was revealed. Maximal effect was observed at C6-AOB concentrations 1 and 10 mcg/ml. Clonal redistribution on ALA manifested by decrease of proportion of clones with high and intermediate values of ALA and increase of proportion of clones with low ALA or its absence.


CONCLUSIONS
Obtained data are perspective for development of measures for control of persisting pathogenic microorganisms by modification of persistence factors with microbial autoregulators.
Bukharin
O V
OV
Perunova
N B
NB
Iavnova
S V
SV
D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006604:Hexylresorcinol / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D009113:Muramidase; D006604:Hexylresorcinol