title: Role of silencing phosphatase of regenerationg liver-3 expression by microRNA interference in the growth of gastric cancer.
abstract: BACKGROUND
  Accumulated evidences demonstrate that phosphatase of regeneration liver-3 (PRL-3) is associated with metastasis of multiple tumor types, and has been validated as a potential therapeutic target for metastasis. High expression of PRL-3 was implicated in lymph node metastasis of gastric cancer. In the present study, we investigated the role of silencing PRL-3 expression by microRNA (miRNA) interference in gastric cancer growth.
  
  
  METHODS
  RNA interference, mediated by recombinant lentivirus expressing artificial PRL-3 miRNA, was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and tumor growth.
  
  
  RESULTS
  Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm(3), significantly smaller compared with controls (P < 0.001).
  
  
  CONCLUSIONS
  Knockdown of PRL-3 significantly suppressed the proliferation of SGC7901 cells in vitro and tumor growth in vivo. PRL-3 plays a key role in the growth of gastric cancer. PRL-3 should be considered as a potential therapeutic target.
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital, Sun Yat-sen University, Gastric Cancer Center of Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: Shi-rong
authors.initials: SR
authors.lastname: Cai
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Chuang-qi
authors.initials: CQ
authors.lastname: Chen
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Wu
authors.forename: Yu-long
authors.initials: YL
authors.lastname: He
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Zhan
authors.forename: Chang-hua
authors.initials: CH
authors.lastname: Zhang
authors.forename: Ji
authors.initials: J
authors.lastname: Cui
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases