title: Comparative proteomic analysis of osteosarcoma cell and human primary cultured osteoblastic cell.
abstract: To identify new biomarkers that facilitate the accurate early diagnosis of osteosarcoma and that may possibly include novel therapeutic candidates, we performed a proteomic approach to compare osteosarcoma cells and human primary cultured osteoblastic cells. Image analysis of silver-stained 2-DE gels revealed that the level of 12 protein spots was significantly different between the two groups of samples (p < .004). After mass spectroscopic identification and database searches, we found that in osteosarcoma cells, the level of HSP70, actin capping protein, ATP synthase, Mthsp75, UQCRC1, Ras-related nuclear protein, UCH-L1, and PRDX4 was elevated. However, the level of pyruvate dehydrogenase E1, Prohibitin, and Annexin V was decreased. Subsequent Western blot analyses of UQCRC1, UCH-L1, and PRDX4 in osteosarcoma tissues confirmed the results obtained by the proteomic analyses. These identified proteins may be potential molecular targets for osteosarcomatous diagnostics and therapeutics.
authors.affiliation: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai JiaoTong University, Shanghai, China. Doctorliu@yahoo.cn
authors.forename: Xudong
authors.initials: X
authors.lastname: Liu
authors.forename: Bingfang
authors.initials: B
authors.lastname: Zeng
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Chunling
authors.initials: C
authors.lastname: Wan
mesh_terms: D015153:Blotting, Western; D001859:Bone Neoplasms / Q000737:chemistry*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D010006:Osteoblasts / Q000737:chemistry*; D012516:Osteosarcoma / Q000737:chemistry*; D054464:Peroxiredoxins / Q000032:analysis; D020543:Proteome; D006180:Proton-Translocating ATPases / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D043222:Ubiquitin Thiolesterase / Q000032:analysis
chemical_list: D018840:HSP70 Heat-Shock Proteins; D009363:Neoplasm Proteins; D020543:Proteome; C482860:UCHL1 protein, human; C515980:PRDX4 protein, human; D054464:Peroxiredoxins; D043222:Ubiquitin Thiolesterase; D006180:Proton-Translocating ATPases