title: Aberration-corrected microscopy for structural biology applications.
abstract: This study explores the potential of a C(s)-corrected transmission electron microscope for structural studies of biological samples, in particular isolated macromolecular complexes. A 300-kV transmission electron microscope, equipped with a C(s) corrector was employed to record sets of images at different defocus and C(s) settings. The experiments were designed to determine whether imaging with large defocus benefits from C(s) correction. Defocus contrast in biological imaging has a stronger influence on image resolution than any other parameter. We find the results are in good agreement with theoretical framework, verifying that the typical imaging conditions required for biological investigations are not affected by C(s) correction.
authors.affiliation: Max-Planck Institute of Biochemistry, Molecular Structural Biology, D-82152 Martinsried, Germany. ziegler@biochem.mpg.de
authors.forename: A
authors.initials: A
authors.lastname: Ziegler
authors.forename: B
authors.initials: B
authors.lastname: Rockel
authors.forename: R
authors.initials: R
authors.lastname: Hegerl
authors.forename: B
authors.initials: B
authors.lastname: Freitag
authors.forename: U
authors.initials: U
authors.lastname: Lücken
authors.forename: J M
authors.initials: JM
authors.lastname: Plitzko
mesh_terms: D007091:Image Processing, Computer-Assisted / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D046988:Proteasome Endopeptidase Complex / Q000648:ultrastructure
chemical_list: D046988:Proteasome Endopeptidase Complex