[Effect of Xuebijing injection on renal high mobility group box-1 protein expression and acute kidney injury in rats after scald injury].
OBJECTIVE
To investigate the change in renal high mobility group box-1 protein (HMGB1) levels, and the effect of Chinese traditional medicine-Xuebijing injection on HMGB1 expression as well as acute kidney injury in rats after scald injury.


METHODS
Wistar rats were subjected to 30% full-thickness scald injury followed with delayed resuscitation. Totally 78 animals were divided into sham scald group (n=18), scald injury group (n=30), and Xuebijing injection treatment group (n=30). All animals were sacrificed at 8, 24, and 72 hours postburn. Renal tissue and blood samples were harvested to determine HMGB1 mRNA as well as protein expression and organ functional parameters. HMGB1 mRNA level was semi-quantitatively measured by the reverse transcription polymerase chain reaction taking GAPDH as an internal standard, and protein expressions of HMGB1 were detected by both Western blot and immunohistochemistry. Serum creatinine (Cr) contents were measured by automatic biochemistry analyzer. In addition, pathological lesions in kidney were observed under light microscope using HE staining.


RESULTS
Compared with sham scald group, both mRNA and protein expressions of HMGB1 were significantly enhanced in the kidney at 8, 24, and 72 hours after scald injury (P<0.05, P<0.01), meanwhile serum Cr contents were markedly increased following acute insults (P<0.05, P<0.01). Treatment with Xuebijing injection could markedly down-regulated renal HMGB1 mRNA expression and protein release at 24 hours and 72 hours (P<0.05, P<0.01), and significantly reduced serum Cr content following scald injury (P<0.05). Many inflammatory cells in renal tissues were observed using light microscope following scald. The histological morphology of kidney lesions was a-HMGB1, a late mediator, appears to be inmeliorated after treatment with Xuebijing injection.


CONCLUSIONS
volved in the pathogenesis of excessive inflammatory response and acute kidney damage. Treatment with Xuebijing injection can inhibit HMGB1 synthesis and release in renal tissues, and may prevent the development of acute kidney injury induced by serious scald injury.
Wang
Qiang
Q
Department of Basic Research, Bumrns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100037, China.
Yao
Yong-ming
YM
Wang
Wen-jiang
WJ
Xian
Li-ming
LM
Dong
Ning
N
Xu
Shan
S
Dou
Ke-feng
KF
D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D002056:Burns / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D024243:HMGB1 Protein / Q000096:biosynthesis*; D007267:Injections; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; C549376:Xuebijing