Sexual development and fertility of Loxl1-/- male mice.
Our objective was to investigate the genitourinary defects and fertility of the male lysyl oxidase-like 1 gene (Loxl1) knockout (Loxl1(-/-)) mouse, with particular attention to fecundity and testicular, epididymal, gubernacular, and penile histopathology, which may lead us to a better understanding of the role of the elastin-homeostasis gene, LOXL1, in male sexual development. Genital morphometric evaluation of 6- to 9-month-old male Loxl1(-/-) mice (n = 26) was compared with C57Bl/6 controls (n = 24). Measurements included: body weight, scrotal development, evidence of feminization (nipples or vaginal pouch), penile malformations, anogenital distance, and absence/presence and size of perineal bulge. Sperm production was estimated using a standardized technique. A breeding program was conducted to determine how much of the infertility observed in Loxl1(-/-) pairs was due to the male factor. Finally, we performed histopathologic comparison of the genitourinary organs of Loxl1(-/-) and control mice. Loxl1(-/-) mice weighed less than their age-matched C57Bl/6 counterparts (P < .001). Size-adjusted perineal bulge was larger (P < .001), and resting location of the gonads was higher intra-abdominally (P = .048) in the Loxl1(-/-) mice. Estimates of daily sperm counts revealed that the Loxl1(-/-) mice had lower sperm production (P = .048). Loxl1(-/-) males bred with control females demonstrated relative fecundity values intermediate between Loxl1(-/-) pairs (lowest fecundity) and control pairs (highest fecundity), suggesting a component of male-factor infertility. No histologic differences were noted using hematoxylin-eosin or specialized elastin staining of the gonads, gubernaculum, and penis. Although further studies are warranted, these findings suggest a subtle and likely multifactorial role of the LOXL1 protein in male sexual development and fertility.
Wood
Hadley M
HM
Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA. damasem@ccf.org
Lee
Una J
UJ
Vurbic
Drina
D
Sabanegh
Edmund
E
Ross
Jonathan H
JH
Li
Tiansen
T
Damaser
Margot S
MS
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D004822:Epididymis / Q000473:pathology; D005260:Female; D005298:Fertility / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010413:Penis / Q000473:pathology; D055101:Semen Analysis; D046468:Sexual Development / Q000235:genetics*; D013076:Sperm Count; D013737:Testis / Q000473:pathology
D000594:Amino Acid Oxidoreductases; C482019:Loxl1 protein, mouse