Sperm cell proteomics.
The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertility, embryo development and heredity. The recent developments in MS have boosted the potential for identification and study of the sperm proteins. Catalogues of hundreds to thousands of spermatozoan proteins in human and in model species are becoming available setting up the basis for subsequent research, diagnostic applications and the development of specific treatments. The present article reviews the available scientific publications dealing with the composition and function of the sperm cell using an MS proteomic approach.
Oliva
Rafael
R
Human Genetics Research Group, IDIBAPS, Faculty of Medicine, University of Barcelona, Barcelona, Spain. roliva@ub.edu
de Mateo
Sara
S
Estanyol
Josep Maria
JM
D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D044127:Epigenesis, Genetic; D018392:Genomic Imprinting; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008954:Models, Biological; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism*
D011506:Proteins