title: Experimental bone biopsies using two bone biopsy needles: quantitative micro-CT analysis of bone specimens.
abstract: OBJECTIVE
  The aim of this study was to investigate whether samples obtained using two kinds of small trephines, 2.4 and 1.8 mm in inner diameter, are sufficient for the quantitative evaluation of metabolic bone disease using micro-computed tomographic (CT) three-dimensional parameter data sets.
  
  
  METHODS
  A total of 19 porcine lumbar vertebrae prior to biopsy and biopsy samples from the use of 2.4- and 1.8-mm trephines were examined using micro-CT imaging. For quantitative analysis, seven three-dimensional structural parameters, including trabecular bone volume, trabecular number, trabecular thickness, trabecular separation, the structure model index, the degree of anisotropy, and the trabecular bone pattern factor, were measured using CtAn software. The difference and agreement between the biopsy samples and the baseline vertebrae specimens before biopsy were assessed using paired t tests and Bland-Altman analysis, respectively.
  
  
  RESULTS
  There were no significant differences between the 2.4-mm samples and the baseline vertebrae specimens for trabecular bone volume, trabecular thickness, and trabecular number, with mean differences of -0.9%, 2.3%, and -3.1%, respectively; there was no significant difference between the 1.8-mm samples and the baseline vertebrae specimens only for trabecular thickness, with a mean difference of 1.9%.
  
  
  CONCLUSIONS
  Samples taken from the use of the 2.4-mm trephine were better for quantitative analysis than those from the use of the 1.8-mm trephine and were acceptable for the quantitative evaluation of trabecular bone volume, trabecular thickness, and trabecular number.
authors.affiliation: Department of Radiology, Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, 110-744, Korea.
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Park
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Hong
authors.forename: Kwang Gi
authors.initials: KG
authors.lastname: Kim
authors.forename: Ja-Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Chan Soo
authors.initials: CS
authors.lastname: Shin
authors.forename: Heung Sik
authors.initials: HS
authors.lastname: Kang
mesh_terms: D000818:Animals; D001707:Biopsy, Needle / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D066298:In Vitro Techniques; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging* / Q000473:pathology*; D009339:Needles; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014057:Tomography, X-Ray Computed / Q000379:methods*