Effects of propofol on pro-inflammatory cytokines and nuclear factor kappaB during polymicrobial sepsis in rats.
Nuclear factor kappa B (NF-kappaB) plays a central role in regulating the transcription of several genes associated with sepsis/septic shock. Therefore, the author investigated the effects of propofol on the plasma tumor necrosis factor alpha and interleukin 6 (TNF-alpha and IL-6) levels and NF-kappaB activation during polymicrobial sepsis in rats. Male Sprague-Dawlay rats were subjected to cecal ligation and puncture (CLP, a model of polymicrobial sepsis) or sham operation. The animals were randomly assigned into four equal groups (n = 10): sham CLP group, CLP group, PPF (propofol) I group and PPF II group. Thirty minutes before CLP, propofol (5 and 10 mg kg(-1) h(-1), respectively) was infused continuously through the left femoral vein cannula in PPF I group or PPF II group, CLP group and sham CLP group receiving 0.9% saline only at the rates of 5 ml kg(-1) h(-1). The right femoral artery was cannulated to monitor mean arterial pressure (MAP) and heart rates (HR). CLP produced progressive hypotension and a first increase followed by a decrease in HR. The plasma TNF-alpha and IL-6 levels and the hepatic NF-kappaB activation significantly increased after CLP alone. Compared with CLP group, propofol treatment reversed hypotension, slightly steadied heartbeats, and decreased the plasma TNF-alpha and IL-6 levels, and significantly suppressed NF-kappaB activation. Propofol has inhibited the hepatic NF-kappaB activation and the pro-inflammatory cytokine response during polymicrobial sepsis in rats.
Song
Xue-Min
XM
The Research Centre of Anesthesiology and Critical Care Medicine, Zhongnan Hospital, Wuhan University, 430071 Wuhan, Hubei Province, China. sxmcl1018@163.com
Wang
Yan-Lin
YL
Li
Jian-Guo
JG
Wang
Cheng-Yao
CY
Zhou
Qing
Q
Zhang
Zong-Ze
ZZ
Liang
Hui
H
D000818:Animals; D002432:Cecum / Q000382:microbiology / Q000601:surgery; D016207:Cytokines / Q000097:blood*; D004195:Disease Models, Animal; D006439:Hemodynamics / Q000187:drug effects; D006500:Hepatic Duct, Common / Q000473:pathology; D006651:Histocytochemistry; D015850:Interleukin-6 / Q000097:blood; D008026:Ligation; D008297:Male; D016328:NF-kappa B / Q000097:blood*; D010538:Peritonitis / Q000097:blood / Q000382:microbiology; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000382:microbiology / Q000473:pathology; D051996:Transcription Factor RelA / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D016207:Cytokines; D015850:Interleukin-6; D016328:NF-kappa B; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D015742:Propofol