A novel N-linked flagellar glycan from Methanococcus maripaludis.
The archaea Methanococcus maripaludis strain Mm900 produces flagella that are glycosylated with an N-linked tetrasaccharide. Mass spectrometric analysis of flagellar tryptic peptides identified a number of tryptic glycopeptides carrying a glycan of mass 1036.4Da, and fragmentation of the glycan oxonium ion indicated that the glycan was a tetrasaccharide. The glycan was purified, following extensive pronase digestion of flagellar filaments, by size-exclusion and anion-exchange chromatography. NMR spectroscopy revealed that the glycan had the following structure: Sug-4-beta-ManNAc3NAmA6Thr-4-beta-GlcNAc3NAcA-3-beta-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-alpha-l-erythro-hexos-5-ulo-1,5-pyranose. This oligosaccharide has significant similarity to the oligosaccharide that was found previously in Methanococcus voltae.
Kelly
John
J
Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6.
Logan
Susan M
SM
Jarrell
Ken F
KF
VanDyke
David J
DJ
Vinogradov
Evgeny
E
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D002240:Carbohydrate Sequence; D005407:Flagella / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D017017:Methanococcus / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D006020:Glycopeptides; D009844:Oligosaccharides