Pullulan acetate nanoparticles prepared by solvent diffusion method for epirubicin chemotherapy.
Pullulan acetate (PA) was synthesized by the reaction of pullulan with acetic anhydride in the presence of pyridine. PA was characterized by Fourier transform infrared (FT-IR) and proton nuclear magnetic resonance ((1)H NMR). A solvent diffusion method was employed in the current work to prepare PA nanoparticles. This technique had some advantages compared with other methods. The particle size increased from 185.7 nm to 423.0 nm with the degree of acetylation increasing from 2.71 to 3.0. Drug-loaded PA nanoparticles were prepared for controlled release of epirubicin (EPI). The drug entrapment and drug content increased with the degree substitution of PA increasing. EPI was released from the nanoparticles in a biphasic profile with a fast release rate in the first 10h followed by a slow release in vitro. A higher cytotoxicity against KB cells was found for EPI-loaded PA nanoparticles in comparison with free EPI. Confocal laser scanning microscopy (CLSM) observations indicate that EPI-loaded nanoparticles were internalized and released in the cytoplasmic compartment.
Zhang
Hui-zhu
HZ
Institute of Biomedical Engineering, Chinese Academy of Medical Science, Peking Union Medical College, The Key Laboratory of Biomedical Material of Tianjin, PO Box 25(204), Tianjin 300192, PR China. huizhu1449@sina.com
Gao
Fu-ping
FP
Liu
Ling-rong
LR
Li
Xue-min
XM
Zhou
Zhi-min
ZM
Yang
Xin-du
XD
Zhang
Qi-qing
QQ
D000089:Acetic Anhydrides / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D015251:Epirubicin / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D005936:Glucans / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared
D000089:Acetic Anhydrides; D004337:Drug Carriers; D005936:Glucans; C031800:acetic anhydride; D015251:Epirubicin; C009109:pullulan