title: In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds.
abstract: In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.
authors.affiliation: REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal.
authors.forename: Marcos
authors.initials: M
authors.lastname: Taveira
authors.forename: David M
authors.initials: DM
authors.lastname: Pereira
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.forename: Anabela
authors.initials: A
authors.lastname: Martins
authors.forename: José A
authors.initials: JA
authors.lastname: Pereira
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D019149:Bioreactors; D001937:Brassica / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000032:analysis; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D000975:Antioxidants; D017077:Culture Media, Conditioned; D010636:Phenols