Preparation and non-invasive in-vivo imaging of anti-adhesion barriers with fluorescent polymeric marks.
Comb-like PEMLn polymers with pendent PEG-PLLA side chains were synthesized as tissue anti-adhesion barriers. The comb-like structure improved the flexibility of the films. Fluorescent polymer-biocompatible polymer guest-host materials were printed on the films as marking dots. Without sacrificing rats on different days after surgery, degradation behaviors of the marked films can be investigated non-invasively in the in-vivo imaging system (IVIS) by monitoring the location of fluorescent signals. Degradation properties of PEML1/G26L35 films were adjusted by incorporating G26L35 oligomers. PEML1 and PEML1/G26L35 films were very effective in preventing post-surgical tissue-adhesions. Degradation behaviors of various films observed in the animal study were consistent with those investigated by the in-vivo imaging method. Fluorescent polymer/biocompatible polymer blends were promising candidates for in-vivo imaging applications.
Hsieh
Shih-Rong
SR
Department of Surgery, Taichung Veterans General Hospital, Taichung 40724, Taiwan.
Chang
Chi-Jung
CJ
Way
Tzong-Der
TD
Kwan
Po-Cheung
PC
Hung
Tsung-Wei
TW
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D008163:Luminescent Measurements / Q000379:methods; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D045663:Quantum Dots; D051381:Rats
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D011108:Polymers