Identification of CD15 as a marker for tumor-propagating cells in a mouse model of medulloblastoma.
The growth of many cancers depends on self-renewing cells called cancer stem cells or tumor-propagating cells (TPCs). In human brain tumors, cells expressing the stem cell marker CD133 have been implicated as TPCs. Here we show that tumors from a model of medulloblastoma, the Patched mutant mouse, are propagated not by CD133(+) cells but by cells expressing the progenitor markers Math1 and CD15/SSEA-1. These cells have a distinct expression profile that suggests increased proliferative capacity and decreased tendency to undergo apoptosis and differentiation. CD15 is also found in a subset of human medulloblastomas, and tumors expressing genes similar to those found in murine CD15(+) cells have a poorer prognosis. Thus, CD15 may represent an important marker for TPCs in medulloblastoma.
Read
Tracy-Ann
TA
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.
Fogarty
Marie P
MP
Markant
Shirley L
SL
McLendon
Roger E
RE
Wei
Zhengzheng
Z
Ellison
David W
DW
Febbo
Phillip G
PG
Wechsler-Reya
Robert J
RJ
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor; D001932:Brain Neoplasms / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism*; D008527:Medulloblastoma / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008817:Mice, Mutant Strains; D016513:Mice, SCID; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D000072079:Patched Receptors; D010455:Peptides / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D015996:Survival Rate; D014407:Tumor Cells, Cultured
D000071916:AC133 Antigen; D015703:Antigens, CD; D051792:Basic Helix-Loop-Helix Proteins; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D053823:Hedgehog Proteins; D016256:Lewis X Antigen; D000072079:Patched Receptors; D010455:Peptides; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C500943:Atoh1 protein, mouse; C000606023:PROM1 protein, human; C000606024:Prom1 protein, mouse