title: Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy.
abstract: Epithelial cancers, including those of the skin and cervix, are the most common type of cancers in humans. Many recent studies have attempted to use Raman spectroscopy to diagnose these cancers. In this paper, Raman spectral markers related to the temporal and spatial effects of cervical and skin cancers are examined through four separate but related studies. Results from a clinical cervix study show that previous disease has a significant effect on the Raman signatures of the cervix, which allow for near 100% classification for discriminating previous disease versus a true normal. A Raman microspectroscopy study showed that Raman can detect changes due to adjacent regions of dysplasia or HPV that cannot be detected histologically, while a clinical skin study showed that Raman spectra may be detecting malignancy associated changes in tissues surrounding nonmelanoma skin cancers. Finally, results of an organotypic raft culture study provided support for both the skin and the in vitro cervix results. These studies add to the growing body of evidence that optical spectroscopy, in this case Raman spectral markers, can be used to detect subtle temporal and spatial effects in tissue near cancerous sites that go otherwise undetected by conventional histology.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Keller
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Kanter
authors.forename: Chad A
authors.initials: CA
authors.lastname: Lieber
authors.forename: Shovan K
authors.initials: SK
authors.lastname: Majumder
authors.forename: Joanne
authors.initials: J
authors.lastname: Hutchings
authors.forename: Darrel L
authors.initials: DL
authors.lastname: Ellis
authors.forename: Richard B
authors.initials: RB
authors.lastname: Beaven
authors.forename: Nicholas
authors.initials: N
authors.lastname: Stone
authors.forename: Anita
authors.initials: A
authors.lastname: Mahadevan-Jansen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D018929:Cell Culture Techniques; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002584:Cervix Uteri / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology*; D012680:Sensitivity and Specificity; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor