Chiral separation of beta-adrenergic blockers on CelluCoat column by HPLC.
The chiral separation of 10 beta-adrenergic blockers (acebutalol, alprenolol, bufuralol, bisoprolol, celiprolol, carazolol, indenolol, metoprolol, oxprenolol and propranolol) was achieved on CelluCoat column (250 mm x 4.6mm, 5 microm particle size). The mobile phases used were (90:10:0.2, v/v/v) and (95:5:0.2, v/v/v) combinations of n-heptane-ethanol-diethylamine, respectively. The flow rates were 0.5, 1.0 and 2.0 mL min(-1) with detection at 225 nm. The capacity (k), selectivity (alpha) and resolution (R(s)) factors were 0.44-12.91, 1.12-2.19 and 1.00-9.50, respectively. The proposed supra-molecular models indicated that the chiral resolution were governed by pi-pi interactions, hydrogen bondings and steric effect.
Ali
Imran
I
Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi 110025, India.
Gaitonde
Vinay D
VD
Aboul-Enein
Hassan Y
HY
Hussain
Afzal
A
D000319:Adrenergic beta-Antagonists / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004583:Electrons; D006860:Hydrogen Bonding; D012997:Solvents; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D012997:Solvents