title: Expression of cell cycle biomarkers and telomere length in papillary thyroid carcinoma: a comparative study between radiation-associated and spontaneous cancers.
abstract: OBJECTIVE
  Radiation exposure during childhood is the only well-established risk factor for papillary thyroid carcinoma (PTC). To better define the biologic profile of radiation-induced and sporadic PTC, we compared in these two groups of PTC the expression of cell cycle regulatory proteins and telomere length.
  
  
  METHODS
  Cell cycle markers (cyclin A, B1, D1, E, and Ki67) were evaluated on 100 PTC specimens (26 radiation-induced and 74 sporadic PTCs). The expression of cell cycle regulators was studied using immunohistochemistry; telomere length heterogeneity was studied using in situ hybridization in a subset of 16 formalin-fixed samples (8 radiation-induced and 8 sporadic PTCs).
  
  
  RESULTS
  At multivariate analysis, only cytoplasmic cyclin E staining was overexpressed in sporadic cases (P = 0.006). The other cell cycle markers and telomere length did not differ significantly between sporadic PTC and radiation-induced PTC.
  
  
  CONCLUSIONS
  These markers cannot be used to differentiate radiation-induced from sporadic PTCs.
authors.affiliation: Laboratoire de Radiobiologie et Oncologie, DSV/DRR/Commissariat à l'Energie Atomique, Villejuif, France.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Achille
authors.forename: Houda
authors.initials: H
authors.lastname: Boukheris
authors.forename: Bernard
authors.initials: B
authors.lastname: Caillou
authors.forename: Monique
authors.initials: M
authors.lastname: Talbot
authors.forename: Florent
authors.initials: F
authors.lastname: de Vathaire
authors.forename: Laure
authors.initials: L
authors.lastname: Sabatier
authors.forename: Chantal
authors.initials: C
authors.lastname: Desmaze
authors.forename: Martin
authors.initials: M
authors.lastname: Schlumberger
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Soria
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D016615:Telomere / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D018797:Cell Cycle Proteins