Electron microscopy in yeast.
The membrane dynamics of autophagy in yeast were resolved mostly by using various electron microscopy (EM) methods combined with the cryofixation technique. In addition, the similarity of the dynamic process between autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway was first clarified through EM studies. In this chapter the application of several EM methods to detect the dynamic events involving cellular structures are described, and examples are provided of the typical images obtained in autophagy and the Cvt pathway.
Baba
Misuzu
M
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan.
D001343:Autophagy / Q000502:physiology*; D002462:Cell Membrane / Q000648:ultrastructure*; D015925:Cryopreservation / Q000379:methods*; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods*; D010588:Phagosomes / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D014617:Vacuoles / Q000648:ultrastructure