Monoclonal antibodies specific to Cucumber mosaic virus coat protein possess DNA-hydrolyzing activity.
Monoclonal antibodies (mAbs) specific to Cucumber mosaic virus coat protein (CMV-CP) were designed from cDNA and deduced amino acid sequences of the light chain genes of 10 out of 14 different hybridoma cell lines. Ten of these mAbs revealed a very restricted germline family VkappaII, within which gene bd2 has identical amino acid sequences with VIPase and an i41SL 1-2 catalytic antibody light chain, both of which possess peptidase activity. Four out of the 14 mAbs illustrated another germline family VkappaIA, within which gene bb1.1 had high homology with BV04-01 light chain mAb, which hydrolyses ssDNA. Interestingly, our mAbs showed DNA-hydrolytic activity at an optimum pH of 4-5, which is a typical pattern of autoimmune diseases in which autoantibodies hydrolyze supercoiled plasmid DNA. This is the first evidence ever that CMV-CP could stimulate catalytic antibodies, which have an identical sequence homology with autoantibodies. Furthermore, the CMV-CP-specific mAbs will be important for isolating antibodies specific to the CPs of bacteria, viruses, cancer cells, etc. that could be used for medical therapy.
Zein
Haggag S
HS
Department of Genetics, Faculty of Agriculture, Cairo University, Giza 121613, Egypt. haggagsalah@gmail.com
da Silva
Jaime A Teixeira
JA
Miyatake
Kazutaka
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D036022:Capsid Proteins / Q000276:immunology*; D002478:Cells, Cultured; D017799:Cucumovirus / Q000276:immunology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D007147:Immunoglobulin Light Chains / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000276:immunology / Q000821:virology
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D007147:Immunoglobulin Light Chains; D004247:DNA; D003851:Deoxyribonucleases