Effects of a Caenorhabditis elegans dauer pheromone ascaroside on physiology and signal transduction pathways.
Daumone is one of the three purified and artificially synthesized components of the Caenorhabditis elegans dauer pheromone. It affects the major signal transduction pathways known to discriminate between developmental arrest at the dauer stage and growth to the adult [the transforming growth factor beta (TGF-beta) and daf-2/IGF1R pathways], just as natural pheromone extracts do. Transcription of daf-7/TGF-beta is reduced in pre-dauer larvae, and nuclear localization of the DAF-16/FOXO transcription factor is increased in embryos and L1 larvae exposed to synthetic daumone. However, daumone does not require the cilia in the amphidial neurons to produce these effects nor does it require the Galpha protein GPA-3 to induce dauer entry, although GPA-3 is required for dauer induction by natural dauer pheromone extracts. Synthetic daumone has physiological effects that have not been observed with natural pheromone. It is toxic at the concentrations required for bioassay and is lethal to mutants with defective cuticles. The molecular and physiological effects of daumone and natural dauer pheromone are only partially overlapping.
Gallo
Marco
M
Department of Medical Genetics, The University of British Columbia, Vancouver, BC, Canada.
Riddle
Donald L
DL
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D051858:Forkhead Transcription Factors; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000254:growth & development; D010675:Pheromones / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D005227:Fatty Acids; D051858:Forkhead Transcription Factors; D010675:Pheromones; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C108831:daf-16 protein, C elegans; C496586:daumone, C elegans