Osteoprotegerin and ligand of receptor activator of nuclear factor kappaB expression in ovariectomized rats during tooth movement.
OBJECTIVE
To test the null hypothesis that increased tooth displacement in ovariectomized rats is not related to differential expressions of OPG and RANKL in the periodontium.


METHODS
Eighty-four 12-week female rats were used; half were ovariectomized and half were not. Three months later, the maxillary first molar was moved mesially. Groups of rats were sacrificed at days 0, 1, 3, 5, 7, 10, and 14 after activation. Tooth movement was measured at each time point. OPG and RANKL expressions were examined through immunohistochemistry.


RESULTS
Ovariectomized and nonovariectomized rats showed three-phase tooth movement. In both groups, OPG expression increased at the tension area and RANKL increased at the pressure area. The OPG/RANKL ratio coincided with tooth movement, especially in the linear phase from 7 to 14 days.


CONCLUSIONS
The null hypothesis is rejected. The increased rate of tooth movement in ovariectomized rats was related to differential expressions of OPG and RANKL.
Tan
Lijun
L
Sichuan University, Sichuan, China.
Ren
Yijin
Y
Wang
Jun
J
Jiang
Lingyong
L
Cheng
Hui
H
Sandham
Andrew
A
Zhao
Zhihe
Z
D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007180:Incisor / Q000473:pathology; D008963:Molar / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D053244:Osteoprotegerin / Q000032:analysis*; D010052:Ovariectomy; D010513:Periodontal Ligament / Q000473:pathology; D011312:Pressure; D053245:RANK Ligand / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013997:Time Factors; D014087:Tooth Movement Techniques
D053244:Osteoprotegerin; D053245:RANK Ligand