Cloning and expression analysis of a small HSP26 gene of Pacific abalone (Haliotis discus hannai).
Heat shock proteins (HSPs) are evolutionally conserved from micro organism to mammals and play important roles in many biological processes including thermal tolerance. We isolated a homologue of small HSP26 (sHSP26) from a subtracted cDNA library of heat shock-treated abalone (Haliotis discus hannai). The abalone sHSP26 encompossed 793 nt, including a coding region of 501 nt. The deduced amino acid sequence of the abalone sHSP26 contained well conserved alpha-crystallin domain and showed overall identities of 27-31% with the other species' sHSP proteins. The abalone sHSP26 transcript was induced by heat shock treatment, but not by cold shock treatment.
Park
Eun Mi
EM
Biotechnology Research Institute, National Fisheries Research and Development Institute, 408-1 Sirang ri, Gijang eup, Gijang gun, Busan 619-902, Republic of Korea.
Kim
Young Ok
YO
Nam
Bo Hye
BH
Kong
Hee Jeong
HJ
Kim
Woo Jin
WJ
Lee
Sang Jun
SJ
Jee
Young Ju
YJ
Kong
In Soo
IS
Choi
Tae Jin
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D005399:Fishes; D049851:Gastropoda / Q000235:genetics*; D020869:Gene Expression Profiling; D006360:Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D051381:Rats; D016415:Sequence Alignment; D012758:Shellfish; D013045:Species Specificity; D013696:Temperature
D006360:Heat-Shock Proteins