title: Telomere dynamics during replicative senescence  are not directly modulated by conditions of oxidative  stress in IMR90 fibroblast cells.
abstract: The replicative lifespan of many cell types is determined by the length of telomeres in the initiating cell population. In 20% oxygen, IMR90 cells have a shorter replicative lifespan compared to that achieved in conditions that lower the levels of oxidative stress. We sought to address the role of telomere dynamics in determining the replicative lifespan of IMR90 cells. We analysed clonal populations cultured in parallel in 3 and 20% oxygen. We observed that, at senescence, telomere length was shorter in 3% oxygen and this was proportional to the lifespan extension. We observed no detectable difference in the rate of telomere erosion in the two culture conditions, however as the cells approached senescence the growth rate of the cultures slowed with a commensurate increase in the rate of telomere erosion. We conclude that, in 20% oxygen senescence of IMR90 is telomere-independent, but telomere-dependent in 3% oxygen.
authors.affiliation: Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Bethan
authors.initials: B
authors.lastname: Britt-Compton
authors.forename: Fiona
authors.initials: F
authors.lastname: Wyllie
authors.forename: Jan
authors.initials: J
authors.lastname: Rowson
authors.forename: Rebecca
authors.initials: R
authors.lastname: Capper
authors.forename: Rhiannon
authors.initials: R
authors.lastname: E Jones
authors.forename: Duncan
authors.initials: D
authors.lastname: M Baird
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D015687:Cell Hypoxia; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*; D014162:Transfection
chemical_list: D000975:Antioxidants; C509186:TERT protein, human; D019098:Telomerase; D010100:Oxygen