title: Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal.
abstract: OBJECTIVE
  Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa).
  
  
  METHODS
  Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n=150, age: 46.6+/-7.7 years), and female controls (n=150, age: 46.8+/-7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction.
  
  
  RESULTS
  Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001; 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N(2),N(2)-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients.
  
  
  CONCLUSIONS
  This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.
authors.affiliation: Life Sciences Division, Korea Institute of Science and Technology, 39-1 Hawolkok-dong, Seoul, Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Cho
authors.forename: Man Ho
authors.initials: MH
authors.lastname: Choi
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Lee
authors.forename: Bong Chul
authors.initials: BC
authors.lastname: Chung
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000652:urine; D000328:Adult; D001943:Breast Neoplasms / Q000601:surgery / Q000652:urine; D002853:Chromatography, Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008408:Mastectomy; D008875:Middle Aged; D009705:Nucleosides / Q000652:urine*; D018384:Oxidative Stress / Q000502:physiology; D011184:Postoperative Period; D017382:Reactive Oxygen Species / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013936:Thymidine / Q000031:analogs & derivatives / Q000652:urine
chemical_list: D009705:Nucleosides; D017382:Reactive Oxygen Species; D006151:Guanosine; C002230:1-methyladenosine; C008940:N(2),N(2)-dimethylguanosine; C018080:5-hydroxymethyl-2'-deoxyuridine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000241:Adenosine; D013936:Thymidine