Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences.
We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.
Furukawa
Kazuhiro
K
Nano Medical Engineering Laboratory, Advance Science Institute, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Abe
Hiroshi
H
Wang
Jin
J
Uda
Miwako
M
Koshino
Hiroyuki
H
Tsuneda
Satoshi
S
Ito
Yoshihiro
Y
D001483:Base Sequence; D003116:Color; D004252:DNA Mutational Analysis / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D016313:Genes, abl / Q000235:genetics; D006801:Humans; D015336:Molecular Probe Techniques; D009841:Oligonucleotides / Q000032:analysis / Q000235:genetics*; D010084:Oxidation-Reduction; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012235:Rhodamines
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012235:Rhodamines