Olive tree wood phenolic compounds with human platelet antiaggregant properties.
Oleuropein and (+)-cycloolivil are natural polyphenolic compounds with a significant radical scavenging activity present in olive tree. We have investigated the antiaggregant effects of oleuropein and (+)-cycloolivil isolated from an ethyl acetate extract of olive tree wood. Oleuropein and (+)-cycloolivil reduced the ability of thrombin to stimulate platelet aggregation. Both compounds reduced thrombin-evoked Ca(2+) release and entry to a similar extent to hydroxytyrosol. This effect was greater in platelets from patients with type 2 diabetes mellitus than in controls. Thrombin-, thapsigargin- and 2,5-di-(tert-butyl)-1,4-hydroquinone (TBHQ)-evoked protein tyrosine phosphorylation, which is involved in Ca(2+) signalling and platelet aggregation, is inhibited by oleuropein and (+)-cycloolivil. oleuropein and (+)-cycloolivil are natural oxygen radical scavengers that reduce thrombin-induced protein tyrosine phosphorylation, Ca(2+) signalling and platelet aggregation. These observations suggest that oleuropein and (+)-cycloolivil may prevent thrombotic complications associated to platelet hyperaggregability and be the base for the development of antiaggregant therapeutic strategies.
Zbidi
Hanene
H
Unité de Recherche de Biochimie, Institut Supérieur de Biotechnologie, 5019-Monastir, Tunisia.
Salido
Sofia
S
Altarejos
Joaquín
J
Perez-Bonilla
Mercedes
M
Bartegi
Aghleb
A
Rosado
Juan A
JA
Salido
Ginés M
GM
D000085:Acetates; D020013:Calcium Signaling / Q000187:drug effects; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011714:Pyrans / Q000302:isolation & purification / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014934:Wood / Q000032:analysis
D000085:Acetates; D016166:Free Radical Scavengers; D057888:Iridoid Glucosides; D039823:Iridoids; D010636:Phenols; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D011714:Pyrans; C542655:cycloolivil; C005975:3,4-dihydroxyphenylethanol; D019000:Phosphotyrosine; C002769:oleuropein; C007650:ethyl acetate; D011505:Protein-Tyrosine Kinases; D013917:Thrombin; D010626:Phenylethyl Alcohol