Expression of mRNA for 5-HT2 receptors and proteins related to inactivation of 5-HT in mouse osteoblasts.
We analyzed the expression of 5-HT(2) receptors and proteins related to inactivation of 5-HT in primary cultures of mouse osteoblasts. The mRNA for the 5-HT(2A) receptor was detectable in anaplastic osteoblasts as well as in differentiated and matured osteoblasts. The mRNA for the 5-HT(2B) receptor and 5-HT transporter was undetectable in anaplastic osteoblasts and became detectable in differentiated and matured osteoblasts. It was suggested that 5-HT might regulate the proliferation of anaplastic osteoblasts through the 5-HT(2A) receptor without control by 5-HT-inactivating mechanisms. The differentiation and maturation of osteoblasts might be regulated by the activation of the 5-HT(2B) receptor under the control of 5-HT inactivation.
Hirai
Takao
T
Department of Molecular Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan.
Tokumo
Kohji
K
Tsuchiya
Daijyu
D
Nishio
Hiroaki
H
D000708:Anaplasia / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism; D044348:Receptors, Serotonin, 5-HT2 / Q000235:genetics*; D012701:Serotonin / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D044402:Receptor, Serotonin, 5-HT2A; D044348:Receptors, Serotonin, 5-HT2; D012701:Serotonin