[YC-1-like potentiation of nitric oxide-dependent activation of soluble guanylyl cyclase by adrenochrome].
The influence of adrenochrome and YC-1 on spermine NONO-induced activation of human soluble guanylyl cyclase was investigated. Adrenochrome (0.1-10 microM) had no effect on the basal activity, but it potentiated in concentration-dependent manner the spermine NONO-induced activation of this enzyme. Adrenochrome, like YC-1, sensitized guanylyl cyclase towards nitric oxide (NO) and produced the leftward shift of spermine NONO concentration responce curve. Addition of adrenochrome decreased the YC-1-induced leftward shift of spermine NONO concentration response curve. Adrenochrome also inhibited (by 63%) the enzyme activation by YC-1. These data demonstrates the possible competition between adrenochrome and YC-1. Thus, synergistic activation of NO-stimulated guanylyl cyclase activity by adrenochrome represents a new biochemical effect of this compound and indicates that adrenochrome may act as an endogenous regulator of NO-dependent stimulation of soluble guanylyl cyclase. This new property of adrenochrome, similar to YC-1, is necessary taking into account, especially under conditions of overproduction of adrenochrome in organism.
Severina
I S
IS
Piatakova
N V
NV
Shchegolev
A Iu
AIu
Sidorova
T A
TA
D000323:Adrenochrome / Q000378:metabolism*; D001792:Blood Platelets / Q000201:enzymology; D004357:Drug Synergism; D004789:Enzyme Activation; D020536:Enzyme Activators / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007191:Indazoles / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D000071756:Soluble Guanylyl Cyclase; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology
D020536:Enzyme Activators; D007191:Indazoles; D020030:Nitric Oxide Donors; D018160:Receptors, Cytoplasmic and Nuclear; C091861:spermine nitric oxide complex; C090937:3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole; D013096:Spermine; D009569:Nitric Oxide; D000323:Adrenochrome; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase