Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic Acid-conjugated dendrimers.
Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.
Landmark
Kevin J
KJ
Programs in Applied Physics, University of Michigan, Ann Arbor, Michigan 48109, USA.
Dimaggio
Stassi
S
Ward
Jesse
J
Kelly
Christopher
C
Vogt
Stefan
S
Hong
Seungpyo
S
Kotlyar
Alina
A
Myc
Andrzej
A
Thomas
Thommey P
TP
Penner-Hahn
James E
JE
Baker
James R
JR
Holl
Mark M Banaszak
MM
Orr
Bradford G
BG
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000493:pharmacokinetics; D003460:Crystallization / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D005290:Ferric Compounds / Q000493:pharmacokinetics; D005492:Folic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D008422:Materials Testing; D008968:Molecular Conformation; D050997:Nanomedicine / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D013499:Surface Properties
D003287:Contrast Media; D004337:Drug Carriers; D005290:Ferric Compounds; D046911:Macromolecular Substances; C000499:ferric oxide; D005492:Folic Acid