Efficient chemical synthesis of AppDNA by adenylation of immobilized DNA-5'-monophosphate.
AppDNA is an intermediate in enzyme-catalyzed DNA ligation reactions, and its efficient enzymatic synthesis requires a donor-template duplex of at least 11 base pairs in length. An efficient chemical synthesis of AppDNA with the coupling of an adenosine 5'-phosphorimidazolidate to an immobilized DNA-5'-monophosphate as the key step is described. The adenylation efficiencies of DNA-5'-monophosphate were excellent for oligonucleotides containing less than 11 nucleotides and at least 50% for oligonucleotides containing 15-25 nucleotides.
Dai
Qing
Q
Department of Biochemistry & Molecular Biology, The University of Chicago, 929 East 57th Street, MC 1028, Chicago, Illinois 60637, USA.
Saikia
Mridusmitas
M
Li
Nan-Sheng
NS
Pan
Tao
T
Piccirilli
Joseph A
JA
D000249:Adenosine Monophosphate / Q000378:metabolism; D002384:Catalysis; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D000249:Adenosine Monophosphate; D004247:DNA