Focused in vivo genetic analysis of implanted engineered myofascial constructs.
Successfully engineering functional muscle tissue either in vitro or in vivo to treat muscle defects rather than using the host muscle transfer would be revolutionary. Tissue engineering is on the cutting edge of biomedical research, bridging a gap between the clinic and the bench top. A new focus on skeletal muscle tissue engineering has led investigators to explore the application of satellite cells (autologous muscle precursor cells) as a vehicle for engineering tissues either in vitro or in vivo. However, few skeletal muscle tissue-engineering studies have reported on successful generation of living tissue substitutes for functional skeletal muscle replacement. Our model system combines a novel aligned collagen tube and autologous skeletal muscle satellite cells to create an engineered tissue repair for a surgically created ventral hernia as previously reported [SA Fann, L Terracio, W Yan, et al., A model of tissue-engineered ventral hernia repair, J Invest Surg. 2006;19(3):193-205]. Several key features we specifically observe are the significant persistence of transplanted skeletal muscle cell mass within the engineered repair, the integration of new tissue with adjacent native muscle, and the presence of significant neovascularization. In this study, we report on our experience investigating the genetic signals important to the integration of neoskeletal muscle tissue. The knowledge gained from our model system applies to the repair of severely injured extremities, maxillofacial reconstructions, and restorative procedures following tumor excision in other areas of the body.
Propst
John T
JT
Department of Surgery, University of South Carolina, Columbia, South Carolina 29209, USA. john.propst@uscmed.sc.edu
Fann
Stephen A
SA
Franchini
Jessica L
JL
Lessner
Susan M
SM
Rose
John R
JR
Hansen
Karyn J
KJ
Terracio
Louis
L
Yost
Michael J
MJ
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006555:Hernia, Ventral / Q000378:metabolism / Q000601:surgery*; D000860:Hypoxia / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D023822:Tissue Engineering; D016378:Tissue Transplantation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D015815:Cell Adhesion Molecules; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D014157:Transcription Factors