Global protein-level responses of Halobacterium salinarum NRC-1 to prolonged changes in external sodium chloride concentrations.
Responses to changes in external salinity were examined in Halobacterium salinarum NRC-1. H. salinarum NRC-1 grows optimally at 4.3 M NaCl and is capable of growth between 2.6 and 5.1 M NaCl. Physiological changes following incubation at 2.6 M NaCl were investigated with respect to growth behavior and proteomic changes. Initial observations indicated delayed growth at low NaCl concentrations (2.6 M NaCl), and supplementation with different sugars, amino acids, or KCl to increase external osmotic pressure did not reverse these growth perturbations. To gain a more detailed insight into the adaptive responses of H. salinarum NRC-1 to changes in salinity, the proteome was characterized using iTRAQ (amine specific isobaric tagging reagents). Three hundred and nine differentially expressed proteins were shown to be associated with changes in the external sodium chloride concentration, with proteins associated with metabolism revealing the greatest response.
Leuko
Stefan
S
Australian Centre for Astrobiology, Bioanalytical Mass Spectrometry Facility, School of Biotechnology and Biomolecular Science, University of New South Wales, NSW, Australia.
Raftery
Mark J
MJ
Burns
Brendan P
BP
Walter
Malcolm R
MR
Neilan
Brett A
BA
D019843:Archaeal Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D019617:Halobacterium salinarum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
D019843:Archaeal Proteins; D020543:Proteome; D012965:Sodium Chloride