[High mobility group box-1 protein induces apoptosis in mouse peritoneal macrophages].
OBJECTIVE
To investigate the effect of high mobility group box-1 protein (HMGB1) on apoptosis of macrophages in mice.


METHODS
The peritoneal macrophages were obtained from female BALB/c mice. After exposure to different doses of HMGB1 (10, 100, 1000, and 10000 ng/ml) for 24 hours, or exposure to 10 000 ng/ml for different time (6, 12, 24, and 48 hours), cells were stained with Annexin V-PE and 7-amino-actinomycin-D (7-AAD), then determined by flow cytometry and laser scanning confocal microscopy, respectively.


RESULTS
Exposed to HMGB1 for 24 hours resulted in a dose-dependent induction of apoptosis, with increased levels of apoptosis observed at 100, 1 000, and 10 000 ng/ml compared with controls [(12.91 +/- 3.89)%, (18.76 +/- 3.41)%, and (39.84 +/- 5.98)%, respectively, vs. (4.49 +/- 1.72)%]. Exposed to HMGB1 at 10000 ng/ml for 6, 12, 24, or 48 hours showed a time-dependent increase in apoptosis rate [(18.33 +/- 4.60)%, (25.02 +/- 5.27)%, (38.30 +/- 6.95)%, and (21.81 +/- 7.96)%]. The percentage of apoptotic cells markedly increased with a peak value at 24 hours after HMGB1 stimulation (P<0.01).


CONCLUSIONS
HMGB1 has a proapoptotic effect on murine peritoneal macrophages.
Liu
Feng
F
Department of Basic Research, Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100037, China.
Yao
Yong-ming
YM
Dong
Ning
N
Xu
Shan
S
Sheng
Zhi-yong
ZY
D000818:Animals; D017209:Apoptosis; D005260:Female; D024243:HMGB1 Protein / Q000502:physiology*; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C
D024243:HMGB1 Protein