Demonstration of lipids in plastic resin-embedded sections of skin material.
Two different fluorescence stains, green: 5-hexadecanoylaminofluorescein, and red: BODIPY(R) 665/676 [(E,E)-3, 5-bis-(4-phenyl-1,3-butadienyl)-4,4-difluoro-4-bora-3a, 4a-diaza-s-indacene, produced good results regarding the demonstration of glycolipids, free fatty acids and triglycerides in mammalian skin material that had been embedded in a water miscible plastic resin (Technovit(R) 7100). In this way, functional aspects of specific structures (epidermal barrier region, sebaceous glands) could be characterized histochemically in the integument of five mammalian species with sparse or dense hair coats.
Meyer
W
W
Institute of Anatomy, University of Veterinary Medicine Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany. wilfried.meyer@tiho-hannover.de
Schmidt
J
J
Busche
R
R
Jacob
R
R
Naim
H Y
HY
D000818:Animals; D002415:Cats; D002417:Cattle; D005456:Fluorescent Dyes; D006736:Horses; D008055:Lipids / Q000032:analysis*; D016613:Plastic Embedding / Q000379:methods*; D051381:Rats; D012867:Skin / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013552:Swine
D005456:Fluorescent Dyes; D008055:Lipids