Novel reticular cyclen-based polymer as gene vector in DNA transfection.
This study provided an experimental evidence for the use of cyclen (1, 4, 7, 10-tetraazacyclododecane)-based polymer for gene delivery. The interesting interaction of the polymer with plasmid DNA was studied by using fluorescence titration, circular dichroism spectra, agarose gel electrophoresis and atomic force microscopy. It was found that polyplex was formed between the polycation and plasmid DNA. The results demonstrated that the cyclen-based polymer could act as non-viral gene vector with relatively low cytotoxicity.
Zhou
Li-Hong
LH
Department of Chemistry, Key Laboratory of Green Chemistry and Technology (Ministry of Education), Sichuan University, Chengdu 610064, China.
Yang
Mu
M
Zhou
Hong
H
Zhang
Ji
J
Li
Kun
K
Xiang
Yong-Zhe
YZ
Wang
Na
N
Tian
Yun-Fei
YF
Yu
Xiao-Qi
XQ
D045744:Cell Line, Tumor; D002942:Circular Dichroism; D000080027:Cyclams; D004247:DNA / Q000737:chemistry*; D005822:Genetic Vectors / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry; D006801:Humans; D018625:Microscopy, Atomic Force; D014162:Transfection
D000080027:Cyclams; D006571:Heterocyclic Compounds; D004247:DNA; C038072:cyclen