[Obtaining of outer membrane protein I of Pseudomonas aeruginosa and assessment of its antigenic properties].
Gene of outer membrane protein I (OprI) of Pseudomonas aeruginosa was cloned in Escherichia coli cells. Synthesized protein OprI contained additional sequence of 6 histidines on the N-terminus, which allowed its chromatographic purification in Ni-agarose. Obtained recombinant ptotein specifically reacted with hyperimmune rabbit serum against whole-cell P. aeruginosa and stimulated synthesis of specific antibodies in immunized mice and rabbits. Obtained hyperimmune rabbit sera against recombinant protein OprI had directive antimicrobial activity against P. aeruginosa.
Gatypova
E V
EV
Zlygostev
S A
SA
Kaloshin
A A
AA
Mikhaĭlova
N A
NA
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D004285:Dogs; D004926:Escherichia coli / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008074:Lipoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D015202:Protein Engineering; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; D008074:Lipoproteins; D011994:Recombinant Proteins; C060507:outer membrane lipoprotein I, Bacteria