title: Identification of novel peptides specifically binding to endometriosis by screening phage-displaying peptide libraries.
abstract: OBJECTIVE
  To search for novel peptides and common binding motif that specifically bind to endometriosis.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Department of Biological Science and Technology in national university.
  
  
  METHODS
  Specimens were divided into [1] ectopic endometrium (n = 10); [2] eutopic endometrium (n = 10).
  
  
  METHODS
  Peptides specifically binding to endometriosis are screened from a phage-displaying peptide library (Ph.D.-12) by using whole-cell screening technique after an adsorption elution amplification procedure.
  
  
  METHODS
  Combinatorial peptide libraries were used to identify small molecules that bind with high affinity to receptor molecules and mimic the interaction with natural ligands. Few pans of positive phage clones with significantly positive signals were identified by ELISA and analyzed by DNA sequencing.
  
  
  RESULTS
  During the biopanning processes, the recovered phage number (10(6) pfu/mL) in parts 1, 2, 3, 4, and 5 of the study were 9, 33, 82, 142, and 169. Nine phages consistently had residue Arg, whereas six clones had a consensus motif of Arg-X-Arg-X-X-X-X-Arg. The biotin-labeled peptide bound to endometriosis cells in a dose-dependent manner, yet the control peptide revealed lesser binding activity.
  
  
  CONCLUSIONS
  The novel motif is associated with higher affinity of endometriosis, which might be useful in endometriosis targeting and as potential antiendometriosis therapies. We provide one potential approach for novel therapies toward endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Chang
authors.forename: Yao-Yuan
authors.initials: YY
authors.lastname: Hsieh
authors.forename: Yu-Kuo
authors.initials: YK
authors.lastname: Wang
authors.forename: Kung-Hao
authors.initials: KH
authors.lastname: Hsu
authors.forename: Horng-Der
authors.initials: HD
authors.lastname: Tsai
authors.forename: Fuu-Jen
authors.initials: FJ
authors.lastname: Tsai
authors.forename: Chih-Sheng
authors.initials: CS
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D016384:Consensus Sequence; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D011446:Prospective Studies; D017422:Sequence Analysis, DNA
chemical_list: D019151:Peptide Library; D010455:Peptides