title: Mutagenic and genotoxic effects of cis-(dichloro)tetraammineruthenium(III) chloride on human peripheral blood lymphocytes.
abstract: Chemotherapeutic agents play an important role in cancer treatment mostly due their systemic action on human organism allowing access to liquid tumors and even metastases. Among these drugs, ruthenium compounds have been showing promising results to treat tumors and represent an important development of new antitumor therapy. This study presents the evaluation of cis-(dichloro)tetraammineruthenium(III) chloride, cis-[RuCl(2)(NH(3))(4)]Cl, genotoxic effects using human peripheral blood lymphocytes cultured in vitro. Mitotic index (MI), chromosome aberrations (CA), and DNA damage using the comet assay were analyzed. MI in human peripheral blood lymphocyte cultures treated with 1, 10, 100, and 1,000 microg mL(-1) cis-[RuCl(2)(NH(3))(4)]Cl were 5.9%, 4.6%, 3.9%, and 0%, respectively. Doxorubicin chloridate was used as the positive control. CA derived from 1, 10, and 100 microg mL(-1) concentrations were defined as spontaneous when compared with the negative control, and at the concentration of 1,000 microg mL(-1), the cell cycle was inhibited (IM = 0%). Results obtained for the comet assay using cis-[RuCl(2)(NH(3))(4)]Cl suggest that this compound has no genotoxic activity against cultured human peripheral blood lymphocytes.
authors.affiliation: Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas, Universidade Federal de Goiás-UFG, ICB I-Sala 200, Goiânia, Goiás 74001-970, Brazil. agarbio@gmail.com
authors.forename: Alessandra de Santana Braga Barbosa
authors.initials: Ade S
authors.lastname: Ribeiro
authors.forename: Cláudio Carlos
authors.initials: CC
authors.lastname: da Silva
authors.forename: Flávia de Castro
authors.initials: Fde C
authors.lastname: Pereira
authors.forename: Aliny Pereira de
authors.initials: AP
authors.lastname: Lima
authors.forename: Cesar Augusto Sam Tiago
authors.initials: CA
authors.lastname: Vilanova-Costa
authors.forename: Simone Santos
authors.initials: SS
authors.lastname: Aguiar
authors.forename: Luiz Alfredo
authors.initials: LA
authors.lastname: Pavanin
authors.forename: Aparecido Divino
authors.initials: AD
authors.lastname: da Cruz
authors.forename: Elisângela de Paula
authors.initials: Ede P
authors.lastname: Silveira-Lacerda
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008677:Metaphase / Q000187:drug effects; D008940:Mitotic Index; D009152:Mutagenicity Tests; D017975:Ruthenium Compounds / Q000008:administration & dosage / Q000633:toxicity*; D055815:Young Adult
chemical_list: C543901:(dichloro)tetraammineruthenium(III); D000970:Antineoplastic Agents; D017975:Ruthenium Compounds