Atypical scrapie in sheep from a UK research flock which is free from classical scrapie.
BACKGROUND
In the wake of the epidemic of bovine spongiform encephalopathy the British government established a flock of sheep from which scrapie-free animals are supplied to laboratories for research. Three breeds of sheep carrying a variety of different genotypes associated with scrapie susceptibility/resistance were imported in 1998 and 2001 from New Zealand, a country regarded as free from scrapie. They are kept in a purpose-built Sheep Unit under strict disease security and are monitored clinically and post mortem for evidence of scrapie. It is emphasised that atypical scrapie, as distinct from classical scrapie, has been recognised only relatively recently and differs from classical scrapie in its clinical, neuropathological and biochemical features. Most cases are detected in apparently healthy sheep by post mortem examination.


RESULTS
The occurrence of atypical scrapie in three sheep in (or derived from) the Sheep Unit is reported. Significant features of the affected sheep included their relatively high ages (6 y 1 mo, 7 y 9 mo, 9 y 7 mo respectively), their breed (all Cheviots) and their similar PRNP genotypes (AFRQ/AFRQ, AFRQ/ALRQ, and AFRQ/AFRQ, respectively). Two of the three sheep showed no clinical signs prior to death but all were confirmed as having atypical scrapie by immunohistochemistry and Western immunoblotting. Results of epidemiological investigations are presented and possible aetiologies of the cases are discussed.


CONCLUSIONS
By process of exclusion, a likely explanation for the three cases of atypical scrapie is that they arose spontaneously and were not infected from an exterior source. If correct, this raises challenging issues for countries which are currently regarded as free from scrapie. It would mean that atypical scrapie is liable to occur in flocks worldwide, especially in older sheep of susceptible genotypes. To state confidently that both the classical and atypical forms of scrapie are absent from a population it is necessary for active surveillance to have taken place.
Simmons
Hugh A
HA
Animal Services Unit, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. h.a.simmons@vla.defra.gsi.gov.uk
Simmons
Marion M
MM
Spencer
Yvonne I
YI
Chaplin
Melanie J
MJ
Povey
Gill
G
Davis
Andrew
A
Ortiz-Pelaez
Angel
A
Hunter
Nora
N
Matthews
Danny
D
Wrathall
Anthony E
AE
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000473:pathology; D005838:Genotype; D007150:Immunohistochemistry / Q000662:veterinary; D011328:Prions / Q000235:genetics; D012608:Scrapie / Q000235:genetics / Q000473:pathology*; D012756:Sheep; D006113:United Kingdom
D011328:Prions