A fluorescence scanning electron microscope.
Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.
Kanemaru
Takaaki
T
Morphology and Core Unit, Kyushu University Hospital, Kyushu, Japan.
Hirata
Kazuho
K
Takasu
Shin-Ichi
S
Isobe
Shin-Ichiro
S
Mizuki
Keiji
K
Mataka
Shuntaro
S
Nakamura
Kei-Ichiro
K
D000818:Animals; D003964:Diaphragm / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar