The effect of Emdogain and 24% EDTA root conditioning on periodontal healing of replanted dog's teeth.
Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain and 24% ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog's teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain; group 2, Emdogain + EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain alone or in combination with 24% EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog's teeth.
Guzmán-Martínez
Nayelli
N
Master in Endodontics Program, Faculty of Dentistry, University of San Luis Potosí, San Luis potosí, México.
Silva-Herzog
Flores Daniel
FD
Méndez
González Verónica
GV
Martín-Pérez
Silvia
S
Cerda-Cristerna
Bernardino Isaac
BI
Cohenca
Nestor
N
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003746:Dental Enamel Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D004285:Dogs; D004492:Edetic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D010513:Periodontal Ligament / Q000187:drug effects*; D011897:Random Allocation; D012038:Regeneration / Q000187:drug effects; D012391:Root Resorption / Q000517:prevention & control*; D014084:Tooth Avulsion / Q000601:surgery; D014090:Tooth Replantation; D014092:Tooth Root / Q000187:drug effects
D002614:Chelating Agents; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D004492:Edetic Acid