title: Evidence for novel tomato seed allergens: IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling.
abstract: Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, D-14467 Potsdam, Germany, Universitat Potsdam GoFORSYS, D-14467 Potsdam, Germany.
authors.forename: Olivia Y
authors.initials: OY
authors.lastname: Bässler
authors.forename: Julia
authors.initials: J
authors.lastname: Weiss
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wienkoop
authors.forename: Karola
authors.initials: K
authors.lastname: Lehmann
authors.forename: Christian
authors.initials: C
authors.lastname: Scheler
authors.forename: Sabine
authors.initials: S
authors.lastname: Dölle
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schwarz
authors.forename: Philipp
authors.initials: P
authors.lastname: Franken
authors.forename: Eckhard
authors.initials: E
authors.lastname: George
authors.forename: Margitta
authors.initials: M
authors.lastname: Worm
authors.forename: Wolfram
authors.initials: W
authors.lastname: Weckwerth
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D000939:Epitopes / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D018551:Solanum lycopersicum / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000276:immunology*; D017434:Protein Structure, Tertiary; D020543:Proteome / Q000378:metabolism; D055314:Seed Storage Proteins / Q000276:immunology*; D012639:Seeds / Q000276:immunology*; D053719:Tandem Mass Spectrometry; D000097793:Legumins
chemical_list: D000485:Allergens; D000939:Epitopes; D007073:Immunoglobulin E; D010940:Plant Proteins; D020543:Proteome; D055314:Seed Storage Proteins; D000097793:Legumins; C015575:vicilin protein, plant