A luminescent beta-cyclodextrin-based Ru(phen)3 complex as DNA compactor, enzyme inhibitor, and translocation tracer.
A beta-cyclodextrin-based Ru(phen)(3) complex (1) has been synthesized and exhibits good luminescent behavior. Atomic force microscopic and scanning electron microscopic studies show that 1 can induce the aggregation of originally circular DNA to toroidal or spherical shapes. The morphology of these DNA aggregates changes following a pathway of naked circular DNA --> toroid with gaps --> solid toroid --> spherical aggregate, depending on the different 1/DNA (w/w) ratios, and their average diameters vary from the nanometer to micrometer scale. Owing to its capability of inducing the aggregation of DNA, 1 can be used as an inhibitor for DNA topoisomerase and DNA cleavage enzymes. Further studies by means of fluorescence microscopy indicate that 1 can also efficiently trace the translocation of DNA into 293T cells (the human embryonic kidney cell line). These observations consequently establish 1 as not only a potential DNA carrier but also a fluorescent DNA probe.
Liu
Yu
Y
Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, P. R. China. yuliu@nankai.edu.cn
Chen
Yong
Y
Duan
Zhong-Yu
ZY
Feng
Xi-Zeng
XZ
Hou
Sen
S
Wang
Chen
C
Wang
Rui
R
D001692:Biological Transport; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*; D012995:Solubility; D059004:Topoisomerase I Inhibitors; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D004791:Enzyme Inhibitors; D049408:Luminescent Agents; D009942:Organometallic Compounds; D059004:Topoisomerase I Inhibitors; D047392:beta-Cyclodextrins; D014867:Water; D012428:Ruthenium; D004247:DNA; D015247:Deoxyribonuclease HindIII; C031215:betadex