title: Associations between pre-employment immunologic and airway mucosal factors and the development of occupational allergy.
abstract: BACKGROUND
  Sensitization to occupational allergens is frequently found in laboratory animal workers (LAWs) and can cause serious health problems. Atopy is a major risk factor for sensitization, but it is considered insufficient to advise against working with animals.
  
  
  OBJECTIVE
  We investigated whether immunologic measures, including serology and cytokine production profiles of blood cells, and parameters for airway inflammation are associated with the development of occupational sensitization.
  
  
  METHODS
  In a prospective cohort study 110 starting LAWs were followed for 2 years. At inclusion, results of health questionnaires, skin test results, lung function measures, methacholine threshold levels, and nasal lavage fluid were obtained. Blood was taken for measuring total IgE and allergen-specific IgE antibodies. Cytokine production profiles were measured in whole blood.
  
  
  RESULTS
  Twenty-two new cases of sensitization were identified during follow-up. In multivariate logistic regression analysis a model including atopy and total IgE level predicted sensitization best. This was corroborated in a separate validation cohort. Parameters for airway inflammation or cytokine production profiles did not further contribute to the prediction of sensitization. Based on these results, pre-employment counseling aimed at applicant LAWs with atopy and a total IgE level of greater than 100 IU/mL might be able to reduce occupational sensitization by up to 45% to 50% with less than 10% false-positive predictions.
  
  
  CONCLUSIONS
  The combination of atopy and total IgE level offered the best model to predict development of occupational sensitization. Other immunologic parameters and parameters of airway inflammation did not contribute significantly.
authors.affiliation: Department of Pulmonology, Academic Medical Center, University of Amsterdam, The Netherlands.
authors.forename: Esmeralda J M
authors.initials: EJ
authors.lastname: Krop
authors.forename: Dick J J
authors.initials: DJ
authors.lastname: Heederik
authors.forename: René
authors.initials: R
authors.lastname: Lutter
authors.forename: Gea
authors.initials: G
authors.lastname: de Meer
authors.forename: Rob C
authors.initials: RC
authors.lastname: Aalberse
authors.forename: Henk M
authors.initials: HM
authors.lastname: Jansen
authors.forename: Jaring S
authors.initials: JS
authors.lastname: van der Zee
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology; D015331:Cohort Studies; D016207:Cytokines / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D016015:Logistic Models; D008137:Longitudinal Studies; D008297:Male; D009784:Occupational Diseases / Q000175:diagnosis* / Q000276:immunology*; D011446:Prospective Studies; D020545:Respiratory Mucosa / Q000276:immunology*; D012307:Risk Factors; D012882:Skin Tests; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D000485:Allergens; D016207:Cytokines; D007073:Immunoglobulin E