title: Polynomial modeling for the identification of lactate minimum velocity by different methods.
abstract: OBJECTIVE
  The lactate minimum (LM) protocol has been used to assess aerobic fitness and to predict exercise intensity associated with the maximal blood lactate steady state. The aim of this study was to compare different methods to identify the lactate minimum velocity (LMV) on cycling.
  
  
  METHODS
  Fourteen male cyclists (26.8+/-4.5 years; 173.2+/-6.1 cm; 67.3+/-5.2 kg; 5,8+/-2.9 years of training) performed the LM test in a velodrome. The protocol consisted of an all out 2 km time trial to elevate blood lactate (bLAC), followed by 8 min of recovery and then 6 bouts of 2 km starting 5 kmxh(-1) below the individual mean velocity for the 6 km performance. The velocity was incremented by 1 kmxh(-1) at each bout with 25 microL of capillary blood being collected for bLAC measurements (YSI 2700 STAT). The LMV was identified visually (vLMV), and by applying a second grade polynomial function on 6 (pLMV(6)) and 3 (pLMV(3)) incremental bouts. Additionally, a method where the bLACx work velocity(-1) quotients (LMVQ) were plotted against the correspondent velocity during the incremental test, identified the LMV by considering 6 (LMVQ(6)) or 3 bouts (LMVQ(3)).
  
  
  RESULTS
  ANOVA showed no differences between vLMV (33.1+/-2.5 kmxh(-1)), pLMV(6) (32.9+/-2.5 kmxh(-1)), pLMV(3) (33.2+/-2.3 kmxh(-1)), LMVQ(6) (32.8+/-2.5 kmxh(-1)) and LMVQ(3) (33.4+/-2.3 kmxh(-1)), with high correlation among them.
  
  
  CONCLUSIONS
  It was possible to identify the LMV by the methods proposed in the present study, even when the results of only 3 bouts of the test were modeled by polynomial function. Such an approach enables a more practical and economical test in addition to minimizing the discomfort due to several blood collections.
authors.affiliation: Unit of Physical Activity and Health, Catholic University of Brasilia, Brasìlia, DF, Brazil. hsimoes@pos.ucb.br
authors.forename: H G
authors.initials: HG
authors.lastname: Simões
authors.forename: W C
authors.initials: WC
authors.lastname: Hiyane
authors.forename: R C
authors.initials: RC
authors.lastname: Sotero
authors.forename: E
authors.initials: E
authors.lastname: Pardono
authors.forename: G M
authors.initials: GM
authors.lastname: Puga
authors.forename: L C J
authors.initials: LC
authors.lastname: Lima
authors.forename: C S G
authors.initials: CS
authors.lastname: Campbell
mesh_terms: D000328:Adult; D015308:Anaerobic Threshold / Q000502:physiology*; D001642:Bicycling / Q000502:physiology*; D005080:Exercise Test; D017079:Exercise Tolerance / Q000502:physiology; D005221:Fatigue; D005500:Follow-Up Studies; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D053580:Muscle Strength / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology*; D014142:Track and Field / Q000502:physiology*
chemical_list: D019344:Lactic Acid