title: p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival.
abstract: p31, the mammalian orthologue of yeast Use1p, is an endoplasmic reticulum (ER)-localized soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) that forms a complex with other SNAREs, particularly syntaxin 18. However, the role of p31 in ER function remains unknown. To determine the role of p31 in vivo, we generated p31 conditional knockout mice. We found that homozygous deletion of the p31 gene led to early embryonic lethality before embryonic day 8.5. Conditional knockout of p31 in brains and mouse embryonic fibroblasts (MEFs) caused massive apoptosis accompanied by upregulation of ER stress-associated genes. Microscopic analysis showed vesiculation and subsequent enlargement of the ER membrane in p31-deficient cells. This type of drastic disorganization in the ER tubules has not been demonstrated to date. This marked change in ER structure preceded nuclear translocation of the ER stress-related transcription factor C/EBP homologous protein (CHOP), suggesting that ER stress-induced apoptosis resulted from disruption of the ER membrane structure. Taken together, these results suggest that p31 is an essential molecule involved in the maintenance of ER morphology and that its deficiency leads to ER stress-induced apoptosis.
authors.affiliation: Laboratory of Molecular Traffic, Department of Molecular and Cellular Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Uemura
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.forename: Takehiro
authors.initials: T
authors.lastname: Aoki
authors.forename: Akitsugu
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Okada
authors.forename: Rika
authors.initials: R
authors.lastname: Hirai
authors.forename: Reiko
authors.initials: R
authors.lastname: Harada
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Mori
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tagaya
authors.forename: Akihiro
authors.initials: A
authors.lastname: Harada
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D004622:Embryo, Mammalian / Q000166:cytology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D017353:Gene Deletion; D020043:Genes, Essential; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D018345:Mice, Knockout; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008954:Models, Biological; D021381:Protein Transport / Q000187:drug effects; D050767:Qc-SNARE Proteins / Q000172:deficiency* / Q000378:metabolism; D050600:SNARE Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / Q000172:deficiency* / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology; D033921:Vesicular Transport Proteins
chemical_list: D050767:Qc-SNARE Proteins; D050600:SNARE Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; C539363:Use1 protein, mouse; D033921:Vesicular Transport Proteins; D014415:Tunicamycin