title: Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1.
abstract: Chitinase was purified from the culture medium of Bacillus licheniformis SK-1 by colloidal chitin affinity adsorption followed by diethylamino ethanol-cellulose column chromatography. The purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular size and pI of chitinase 72 (Chi72) were 72 kDa and 4.62 (Chi72) kDa, respectively. The purified chitinase revealed two activity optima at pH 6 and 8 when colloidal chitin was used as substrate. The enzyme exhibited activity in broad temperature range, from 40 to 70 degrees C, with optimum at 55 degrees C. It was stable for 2 h at temperatures below 60 degrees C and stable over a broad pH range of 4.0-9.0 for 24 h. The apparent K (m) and V (max) of Chi72 for colloidal chitin were 0.23 mg ml(-1) and 7.03 U/mg, respectively. The chitinase activity was high on colloidal chitin, regenerated chitin, partially N-acetylated chitin, and chitosan. N-bromosuccinamide completely inhibited the enzyme activity. This enzyme should be a good candidate for applications in the recycling of chitin waste.
authors.affiliation: Biochemistry Department, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Sanya
authors.initials: S
authors.lastname: Kudan
authors.forename: Rath
authors.initials: R
authors.lastname: Pichyangkura
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D002688:Chitinases / Q000302:isolation & purification* / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002688:Chitinases