title: In vivo off-resonance saturation magnetic resonance imaging of alphavbeta3-targeted superparamagnetic nanoparticles.
abstract: Magnetic resonance imaging is a powerful clinical imaging technique that allows for noninvasive tomographic visualization of anatomic structures with high spatial resolution and soft tissue contrast. However, its application in molecular imaging of cancer has been limited by the lack of sensitivity and detection accuracy in depicting the biochemical expression of these diseases. Here, we combine an ultrasensitive design of superparamagnetic polymeric micelles (SPPM) and an off-resonance saturation (ORS) method to enhance the imaging efficacy of tumor biomarkers in vivo. SPPM nanoparticles encoded with cyclic(RGDfK) were able to target the alpha(v)beta(3)-expressing microvasculature in A549 non-small cell lung tumor xenografts in mice. ORS greatly improved tumor detection accuracy over the conventional T(2)*-weighted method by its ability to turn "ON" the contrast of SPPM. This combination of ORS imaging with a tumor vasculature-targeted, ultrasensitive SPPM design offers new opportunities in molecular imaging of cancer.
authors.affiliation: Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas 35790, USA.
authors.forename: Chalermchai
authors.initials: C
authors.lastname: Khemtong
authors.forename: Chase W
authors.initials: CW
authors.lastname: Kessinger
authors.forename: Jimin
authors.initials: J
authors.lastname: Ren
authors.forename: Erik A
authors.initials: EA
authors.lastname: Bey
authors.forename: Su-Geun
authors.initials: SG
authors.lastname: Yang
authors.forename: Jagadeesh Setti
authors.initials: JS
authors.lastname: Guthi
authors.forename: David A
authors.initials: DA
authors.lastname: Boothman
authors.forename: A Dean
authors.initials: AD
authors.lastname: Sherry
authors.forename: Jinming
authors.initials: J
authors.lastname: Gao
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002196:Capillaries / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply / Q000473:pathology*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000032:analysis* / Q000378:metabolism; D008175:Lung Neoplasms / Q000098:blood supply / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D008833:Microcirculation; D053758:Nanoparticles; D019047:Phantoms, Imaging; D011971:Receptors, Immunologic / Q000032:analysis; D018000:Receptors, Peptide / Q000032:analysis; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous; D014316:Tritium
chemical_list: D014408:Biomarkers, Tumor; D039302:Integrin alphaVbeta3; D008823:Micelles; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C051382:arginyl-glycyl-aspartic acid directed cell adhesion receptor; D014316:Tritium