Structure-activity relationships in linear oligoester ion-channels.
The ion transport activity of eighteen linear oligoesters was assessed using a quantitative fluorescence assay to monitor the collapse of a pH gradient across a vesicle bilayer membrane. Significant activity was associated with compounds that have extended lengths comparable to the thickness of the bilayer membrane, and with the most hydrophilic members of the compounds surveyed. Substantial differences in activity between constitutional isomers were also observed. The aggregation of active compounds in aqueous solution was detected by a pyrene fluorescence probe, and subsequently detected as a rate-limiting step in a sequential vesicle experiment.
Fyles
Thomas Murray
TM
Department of Chemistry, University of Victoria, PO Box 3066, Victoria, BC, CanadaV8W 3V6. tmf@uvic.ca
Luong
Horace
H