The effect of lactic acid bacteria isolates on the urinary tract pathogens to infants in vitro.
Urinary tract infections are common clinical problems in children, even though lots of treatment strategies have been tried. Many studies of the application of probiotics for urinary tract infection in female adults exist, but there is a lack of studies in children. The aims of this study were to screen probiotic strains for inhibiting the uropathogens in vitro, to find candidates for in vivo study. Nine strains of E. coli were isolated from children with urinary tract infection and six uropathogens were obtained from Korean Collection for Type Cultures and American Type Culture Collection. Also 135 lactic acid bacteria (LAB) strains were isolated from healthy children, and were identified through physiologic, biochemical methods, 16S rDNA PCR, and data analysis. And with agar disk diffusion assay technique the antimicrobial activities of these LAB strains against those uropathogens were examined. Three strains of separated LAB strains demonstrated major antimicrobial activity against all the uropathogens. In the agar disk diffusion assay technique, antimicrobial activities increased most in the 4th day culture broth with separated Lactobacillus. In summary, some LAB can be used as candidates to develop the probiotic microorganisms that inhibit uropathogens in children, and are expected to be applied to treatment and prevention of pediatric urinary tract infection.
Lim
In Seok
IS
Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, Korea. ctrhslee@hotmail.com
Lee
Ho Seok
HS
Kim
Won Yong
WY
D000362:Agar / Q000737:chemistry; D000890:Anti-Infective Agents / Q000494:pharmacology; D002648:Child; D003470:Culture Media / Q000378:metabolism; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism*; D005243:Feces; D006801:Humans; D066298:In Vitro Techniques; D007723:Korea; D019344:Lactic Acid / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000628:therapy
D000890:Anti-Infective Agents; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D000362:Agar