Single-point mutations causing more than 100-fold underestimation of human immunodeficiency virus type 1 (HIV-1) load with the Cobas TaqMan HIV-1 real-time PCR assay.
Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.
Korn
Klaus
K
Institute of Clinical and Molecular Virology, National Reference Center for Retroviruses, University of Erlangen-Nuremberg, Erlangen, Germany. klaus.korn@viro.med.uni-erlangen.de
Weissbrich
Benedikt
B
Henke-Gendo
Cornelia
C
Heim
Albert
A
Jauer
Christin Mariel
CM
Taylor
Ninon
N
Eberle
Josef
J
D001483:Base Sequence; D003951:Diagnostic Errors; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019562:Viral Load / Q000379:methods*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus