Electronic detection of DNA mutation based on strand exchange reaction.
A new approach to electronic detection of a single base mismatch is described. The assay involves the electrochemical measurements of DNA strand exchange reactions (SERs) between electrode-bound redox-modified DNA duplex and target DNA, where the sequence of redox-modified DNA is exchangeable to that of the target DNA. The presence of a single base mismatch can be determined from the slower SER rates compared with fully matched DNA.
Watanabe
Mariko
M
Department of Materials Science and Chemistry, University of Hyogo, Himeji, Hyogo, Japan.
Kumamoto
Satoshi
S
Nakamaura
Mitsunobu
M
Yamana
Kazushige
K
D000595:Amino Acid Sequence; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction
D015342:DNA Probes; D006046:Gold; D004247:DNA