Anti-HEV antibodies in domestic animal species and rodents from Spain using a genotype 3-based ELISA.
A truncated ORF2 capsid HEV antigen derived from a genotype 3 strain was developed in insect cells and insect larvae, and compared with the Sar55 antigen and a commercial ELISA. The antigen expressed in insect cells showed a better correlation with Sar55 (kappa value (k)=0.84) than the insect larvae antigen (k=0.69), and a better reproducibility as indicated by the intra and interplate variation coefficients. Commercial ELISA designed for human diagnosis but adapted to animal use using specific secondary antibodies demonstrated to have a very low sensitivity. The insect cell expressed antigen was used to develop an ELISA to detect anti-HEV-IgG in serum samples of different domestic animal and rodents. Seropositivity in the studied animal populations was 71.4% for pigs, 0.60% for goats, 1.92% for sheep, and 11.11% for cats. None of the 1170 cattle samples or 166 rodent samples analyzed was positive.
Peralta
Bibiana
B
Centre de Recerca en Sanitat Animal (CRESA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Barcelona, Spain. bibiana.peralta@cresa.uab.es
Casas
Maribel
M
de Deus
Nilsa
N
Martín
Marga
M
Ortuño
Anna
A
Pérez-Martín
Eva
E
Pina
Sonia
S
Mateu
Enric
E
D000818:Animals; D000829:Animals, Domestic; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005838:Genotype; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000097:blood; D007313:Insecta / Q000821:virology; D007814:Larva / Q000821:virology; D012377:Rodentia; D012680:Sensitivity and Specificity; D013030:Spain / Q000453:epidemiology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G