title: Functionalization of luminescent aminated particles for facile bioconjugation.
abstract: For labeling proteins (streptavidin and fibronectin) by luminescent aminated nanoparticles, an interesting strategy that requires neither activation nor chemical pre- or post-treatment was explored. Because biomolecules are easily rendered luminescent after reaction with organic dyes carrying isothiocyanate moiety, phenylene diisothiocyanate (DITC) was used for covalently binding proteins onto luminescent hybrid gadolinium oxide nanoparticles whose ability to combine imaging and therapy was recently demonstrated.
authors.affiliation: Laboratoire de Physico-Chimie des Materiaux Luminescents, UMR 5620 CNRS-Universite Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France.
authors.forename: Anne-Charlotte
authors.initials: AC
authors.lastname: Faure
authors.forename: Céline
authors.initials: C
authors.lastname: Hoffmann
authors.forename: Rana
authors.initials: R
authors.lastname: Bazzi
authors.forename: Fabrice
authors.initials: F
authors.lastname: Goubard
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pauthe
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Pascal
authors.initials: P
authors.lastname: Perriat
authors.forename: Stéphane
authors.initials: S
authors.lastname: Roux
authors.forename: Olivier
authors.initials: O
authors.lastname: Tillement
mesh_terms: D001710:Biotin / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D003460:Crystallization; D005353:Fibronectins / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods; D011522:Protons; D012833:Siloxanes / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
chemical_list: D004396:Coloring Agents; D005353:Fibronectins; D011522:Protons; D012833:Siloxanes; C030581:gadolinium oxide; D001710:Biotin; D019809:Streptavidin; D005682:Gadolinium