title: Ion-transfer voltammetric determination of the beta-blocker propranolol in a physiological matrix at silicon membrane-based liquid|liquid microinterface arrays.
abstract: In this work, the ion-transfer voltammetric detection of the protonated beta-blocker propranolol in artificial saliva is presented. Cyclic voltammetry, differential pulse voltammetry, and differential pulse stripping voltammetry (DPSV) were employed in the detection of the cationic drug based on ion-transfer voltammetry across arrays of microinterfaces between artificial saliva and an organogel phase. It was found that the artificial saliva matrix decreased the available potential window for ion-transfer voltammetry at this liquid|liquid interface but transfer of protonated propranolol was still achieved. The DPSV method employed a preconditioning step as well as a preconcentration step followed by analytical signal generation based on the back-transfer of the drug across the array of microinterfaces. The DPSV peak current response was linear with drug concentration in the artificial saliva matrix over the concentration range of 0.05-1 microM (i(p) = -8.13 (nA microM(-1))(concentration) + 0.07 (nA), R = 0.9929, n = 7), and the calculated detection limit (3s(b)) was 0.02 microM. These results demonstrate that DPSV at arrays of liquid|liquid microinterfaces is a viable analytical approach for pharmaceutical determinations in biomimetic matrixes.
authors.affiliation: Tyndall National Institute, Lee Maltings, University College, Cork, Ireland.
authors.forename: Courtney J
authors.initials: CJ
authors.lastname: Collins
authors.forename: Damien W M
authors.initials: DW
authors.lastname: Arrigan
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D017136:Ion Transport; D011433:Propranolol / Q000032:analysis*; D012825:Silicon / Q000737:chemistry*
chemical_list: D000319:Adrenergic beta-Antagonists; D011433:Propranolol; D012825:Silicon