Monitoring the role of autophagy in C. elegans aging.
Autophagy plays crucial roles in many biological processes, and recent research points to a possibly conserved role for autophagy in the process of organismal aging. Experiments in the nematode C. elegans suggest that autophagy may be required specifically for longevity pathways that are regulated by environmental signals. Known longevity genes can be assigned to four major longevity pathways/processes: insulin/IGF-1 signaling, dietary restriction, protein translation, and mitochondrial respiration. Of these, reduced insulin/IGF-1 signaling and dietary restriction, but not protein translation inhibition, appear to rely on autophagy to increase life span. Multiple experimental approaches have been used to study autophagy in the context of aging in C. elegans. This chapter describes techniques used to address the link between aging and autophagy in C. elegans. Specifically, we summarize how to examine organismal life span in various longevity mutants and how to visually detect autophagy and auto-lysosomal formation in C. elegans.
Meléndez
Alicia
A
Queens College-CUNY, Department of Biology, Flushing, New York, USA.
Hall
David H
DH
Hansen
Malene
M
D000375:Aging / Q000502:physiology*; D000818:Animals; D001343:Autophagy / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D017173:Caenorhabditis elegans / Q000166:cytology / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D031204:Caloric Restriction; D019069:Cell Respiration / Q000502:physiology; D007328:Insulin / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008136:Longevity / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism; D010588:Phagosomes / Q000378:metabolism; D010860:Pigments, Biological / Q000378:metabolism; D014176:Protein Biosynthesis; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D029742:Caenorhabditis elegans Proteins; D007328:Insulin; D010860:Pigments, Biological; D011993:Recombinant Fusion Proteins; D007334:Insulin-Like Growth Factor I