The maturation inhibitor bevirimat (PA-457) can be active in patients carrying HIV type-1 non-B subtypes and recombinants.
BACKGROUND
Bevirimat (PA-457) is the first candidate of a new family of antiretroviral drugs, the maturation inhibitors. Its action is based on disruption of the protease cleavage of the Gag precursor region. Six resistance mutations have been described and analysed in virus from both treatment-naive and protease inhibitor (PI)-experienced patients, but only in the subtype B of HIV type-1 (HIV-1) virus. Thus, genotypic resistance in non-B subtypes still requires analysis.


METHODS
HIV-1 sequences of different subtypes (54 B, 81 non-B and recombinants) were analysed for the presence of resistance mutations to bevirimat, located within the capsid (CA) protein and spacer peptide 1 (SP1) cleavage site.


RESULTS
No resistance mutations were found, although polymorphisms appeared in some CA-SP1 residues. The C-terminal CA protein and the N-terminal SP1 presented high conservation, whereas C-terminal SP1 was highly variable in sequence and length, with unknown influence in resistance acquisition.


CONCLUSIONS
The results of the present study confirm an absolute conservation of the residues involved in bevirimat in vitro resistance in a large panel of HIV-1 subtypes and recombinants from both treatment-naive and PI-experienced patients. Treatment alone seemed to increase the polymorphisms account in CRF02_AG recombinant sequences; however, the influence of natural polymorphisms needs to be explored.
Yebra
Gonzalo
G
HIV-1 Molecular Epidemiology Laboratory, Department of Microbiology, Hospital Universitario Ramón y Cajal and CIBERESP, Madrid, Spain.
Holguín
Africa
A
D019380:Anti-HIV Agents / Q000627:therapeutic use*; D036022:Capsid Proteins / Q000235:genetics; D024921:Drug Resistance, Multiple, Viral; D015497:HIV-1 / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D009154:Mutation; D011110:Polymorphism, Genetic; D013386:Succinates / Q000627:therapeutic use*; D014315:Triterpenes / Q000627:therapeutic use*; D053978:Pentacyclic Triterpenes
D019380:Anti-HIV Agents; D036022:Capsid Proteins; D013386:Succinates; D014315:Triterpenes; C479935:bevirimat; D053978:Pentacyclic Triterpenes