title: Rapid and sensitive assay for trantinterol, a novel beta(2)-adrenoceptor agonist, in human plasma using liquid chromatography-tandem mass spectrometry.
abstract: A rapid and sensitive assay for trantinterol, a novel beta(2)-adrenoceptor agonist, in human plasma has been developed. Samples containing the analyte and internal standard, clenbuterol, were analyzed by liquid chromatography-tandem mass spectrometry after liquid-liquid extraction with diethyl ether:dichloromethane (60:40, v/v). Separation was performed on a Venusil MP C(18) column (50 mm x 4.6 mm, 5 microm) using methanol:1% formic acid (50:50, v/v) as mobile phase and monitored by multiple reaction monitoring of the precursor-to-product ion transitions of trantinterol at m/z 311.2-->238.1 and clenbuterol at m/z 277.2 --> 203.1. The total run time was only 1.5 min and the method was linear over the concentration range 1-1000 pg/mL with a lower limit of quantitation of 1 pg/mL. Intra- and inter-day precisions (relative standard deviation) were below 7% and 12%, respectively, with accuracy (relative error) below 8%. The method was successfully applied to a pharmacokinetic study involving oral administration of a 50 microg trantinterol tablet to healthy volunteers.
authors.affiliation: Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun, China.
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Liu
authors.forename: Jiwen
authors.initials: J
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Weisong
authors.initials: W
authors.lastname: Yu
authors.forename: John Paul
authors.initials: JP
authors.lastname: Fawcett
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D058647:Adrenergic alpha-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000097:blood* / Q000493:pharmacokinetics; D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002976:Clenbuterol / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008297:Male; D011786:Quality Control; D053719:Tandem Mass Spectrometry
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; C542064:trantinterol; D002976:Clenbuterol