title: Identification of embryonic pancreatic genes using Xenopus DNA microarrays.
abstract: The pancreas is both an exocrine and endocrine endodermal organ involved in digestion and glucose homeostasis. During embryogenesis, the anlagen of the pancreas arise from dorsal and ventral evaginations of the foregut that later fuse to form a single organ. To better understand the molecular genetics of early pancreas development, we sought to isolate markers that are uniquely expressed in this tissue. Microarray analysis was performed comparing dissected pancreatic buds, liver buds, and the stomach region of tadpole stage Xenopus embryos. A total of 912 genes were found to be differentially expressed between these organs during early stages of organogenesis. K-means clustering analysis predicted 120 of these genes to be specifically enriched in the pancreas. Of these, we report on the novel expression patterns of 24 genes. Our analyses implicate the involvement of previously unsuspected signaling pathways during early pancreas development. Developmental Dynamics 238:1455-1466, 2009. (c) 2009 Wiley-Liss, Inc.
authors.affiliation: Department of Developmental and Cell Biology, University of California, Irvine, California, USA.
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Hayata
authors.forename: Ira L
authors.initials: IL
authors.lastname: Blitz
authors.forename: Nahoko
authors.initials: N
authors.lastname: Iwata
authors.forename: Ken W Y
authors.initials: KW
authors.lastname: Cho
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016000:Cluster Analysis; D003920:Diabetes Mellitus / Q000503:physiopathology; D005346:Fibroblast Growth Factors / Q000378:metabolism; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010179:Pancreas / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology / Q000235:genetics
chemical_list: D015415:Biomarkers; D051157:Frizzled Receptors; D053823:Hedgehog Proteins; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D043562:Receptors, G-Protein-Coupled; D051153:Wnt Proteins; D029867:Xenopus Proteins; C492385:fzd10a protein, Xenopus; D005346:Fibroblast Growth Factors