Anti-inflammatory effects of talosin A via inhibition of NF-kappaB activation in lipopolysaccharide-stimulated RAW264.7 cells.
Aim of work To determine whether talosin A inhibits production of pro-inflammatory cytokines and nitric oxide (NO) in lipopolysaccharide (1 mug/ml)-stimulated RAW 264.7 macrophages. Talosin A (10 and 50 mug/ml) significantly reduced LPS-induced overproduction of tumor necrosis factor-alpha, interleukin IL-1beta, -6 and NO in a dose-dependent manner (P < 0.01). Talosin A had a direct NO-scavenging activity in the cell-free system. In RT-PCR analysis, gene expressions were inhibited at a transcriptional level. Moreover, the activation of nuclear factor-kappa B (NF-kappaB) was significantly suppressed by talosin A in LPS-stimulated macrophage cells (P < 0.05). Therefore, we confirmed anti-inflammatory activity of talosin A was via suppression of pro-inflammatory cytokines, NO production and NF-kappaB activation, suggesting a therapeutic candidate for inflammatory disorders.
Hwang
Youn-Hwan
YH
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea.
Kim
Myoung-Seok
MS
Song
In-Bae
IB
Lim
Jong-Hwan
JH
Park
Byung-Kwon
BK
Yun
Hyo-In
HI
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006027:Glycosides / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D006027:Glycosides; D007529:Isoflavones; D016328:NF-kappa B; C516691:genistein 7-(6-deoxytalopyranoside); D009569:Nitric Oxide