title: Understanding membrane fusion combining experimental and simulation studies.
abstract: Target membrane proteins, SNAP-25 and syntaxin (t-SNARE), and secretory vesicle-associated membrane protein (v-SNARE), are part of the conserved protein complex involved in fusion of opposing bilayers in biological systems in the presence of calcium. It is known that SNARE interaction allows opposing bilayers to come close within a distance of approximately 2.8 A, enabling calcium to drive membrane fusion. X-ray diffraction studies and light scattering measurements performed in SNARE-reconstituted liposomes demonstrate that when reconstituted t-SNARE- and v-SNARE-vesicles are allowed to interact prior to calcium addition, membrane fusion fail to occur. These results suggest that hydrated calcium ions are too large (approximately 6 A) to fit between the SNARE-apposed bilayer space, and as a result, unable to induce membrane fusion. In the presence of calcium, however, t-SNARE vesicles interact with v-SNARE vesicles, allowing formation of calcium-phosphate bridges between the opposing bilayers, resulting in the expulsion of coordinated water at the phosphate of the phospholipid head-groups, and due to disruption of the water shell around the calcium ion, enabling lipid mixing and membrane fusion. This hypothesis when tested using atomistic molecular dynamic simulations in the isobaric-isothermal ensemble using hydrated dimethylphosphate anions (DMP(-)) and calcium cations, demonstrate formation of DMP-Ca(2+) complexes and the consequent removal of water, supporting the hypothesis. As a result of Ca(2+)-DMP self-assembly, the distance between anionic oxygens between the two DMP molecules is reduced to 2.92 A, which is in agreement with the 2.8 A SNARE-induced apposition established between opposing lipid bilayers, reported from X-ray diffraction measurements.
authors.affiliation: Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: Bhanu P
authors.initials: BP
authors.lastname: Jena
mesh_terms: D001671:Biochemistry / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D003198:Computer Simulation; D008027:Light; D008081:Liposomes / Q000378:metabolism; D008561:Membrane Fusion; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D012542:Scattering, Radiation; D013572:Synaptic Vesicles / Q000378:metabolism; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D008081:Liposomes; D009943:Organophosphorus Compounds; C007477:dimethyl phosphate; D010100:Oxygen; D002118:Calcium