title: [Expression and significance of vascular cell adhesion molecule-1 in human oral squamous cell carcinoma].
abstract: OBJECTIVE
  To study the expression and the location of vascular cell adhesion molecule-1 (VCAM-1) gene and its clinical significance in human oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  In situ hybridization, PV-9000 polymer detection system for immunohistochemical staining was used to detect the expression and the location of VCAM-1 mRNA and VCAM-1 protein in 48 cases of OSCC and 10 cases of normal controls. Statistical analysis was performed using chi-square test in SPSS 13.0.
  
  
  RESULTS
  VCAM-1 protein was mainly expressed in tumor cell cytoplasm and membrane, VCAM-1 mRNA was mainly expressed in tumor cell cytoplasm. The expression rate of VCAM-1 mRNA and VCAM-1 protein was significantly higher in OSCC than that in normal oral mucosa (P<0.01). The expression of VCAM-1 mRNA was positively correlated with that of VCAM-1 protein (P<0.01). In the clinicopathologic factors, lymph node metastasis and depth of infiltration were closely correlated with VCAM-1 expression (P<0.01). The expression of VCAM-1 was significantly higher in tumor with lymph node metastasis than in tumor without lymph node metastasis (P<0.01).
  
  
  CONCLUSIONS
  Overexpression of VCAM-1 gene in OSCC may play a potential role in the development of OSCC. The overexpression of VCAM-1 gene in OSCC may be related to the tumor infiltration and metastasis.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
authors.forename: Le-gang
authors.initials: LG
authors.lastname: Sun
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Song
authors.forename: You-cheng
authors.initials: YC
authors.lastname: Yang
authors.forename: Li-fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell; D006801:Humans; D017403:In Situ Hybridization; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009061:Mouth Mucosa; D009062:Mouth Neoplasms; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1