title: The identification of a caffeine-induced Ca2+ influx pathway in rat primary sensory neurons.
abstract: Caffeine-induced Ca2+ transients (CICTs) in rabbit nodose ganglion neurons (NGNs) are produced by two distinct mechanisms: release from intracellular stores via ryanodine receptors and Ca2+ influx across the plasma membrane, due to activation of an unknown receptor. In isolated rat NGNs, we used single-cell microfluorimetry to measure changes in intracellular Ca2+ and to test whether TRPV1 receptors underlie the Ca2+ influx pathway. Caffeine (10 mM) evoked CICTs in all NGNs tested (n = 47) averaging 365 +/- 32 nM. CICTs were partially dependent upon a Ca2+ influx pathway that ranged between 33% and 98% of the total Ca2+ transient. Application of two selective TRPV1 antagonists significantly attenuated CICTs. The peak average amplitudes of CICTs in Ca2+-free Locke solution and Ca2+-free Locke solution with IRTX or with BCTC were not significantly different from one another (n = 5 and 7, respectively). These observations suggest that caffeine can induce Ca2+ influx by activating TRPV1 channels.
authors.affiliation: Department of Pathology, School of Medicine, Fluminense Federal University, 303 Marquês do Paraná Street, Room 4, Niterói, RJ 24033-900, Brazil. jpldaher@hotmail.com
authors.forename: João Paulo L
authors.initials: JP
authors.lastname: Daher
authors.forename: Tony D
authors.initials: TD
authors.lastname: Gover
authors.forename: Thais H V
authors.initials: TH
authors.lastname: Moreira
authors.forename: Vânia G S
authors.initials: VG
authors.lastname: Lopes
authors.forename: Daniel
authors.initials: D
authors.lastname: Weinreich
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000378:metabolism; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000697:Central Nervous System Stimulants; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D002110:Caffeine