title: Stenotrophomonas chelatiphaga sp. nov., a new aerobic EDTA-degrading bacterium.
abstract: A new ethylenediaminetetraacetic acid (EDTA)-utilizing gammaproteobacterial strain LPM-5(T) was isolated from municipal sewage sludge. Aerobic, gram-negative, motile rods multiply by binary fission. Neutrophilic and mesophilic, these are unable to grow in the presence of 3% NaCl (w/v), and unable to reduce nitrate to nitrite, and are oxidase and catalase positive, but lipase negative. The major cellular fatty acids are C(i15:0), C(a15:0) and C(16:1w7c). The dominant phospholipids are phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol (cardiolipin). The DNA G+C content is 68.3mol% (T(m)). The 16S rRNA gene sequence analysis showed a high similarity of strain LPM-5(T) to the species members of genus Stenotrophomonas: S. maltophilia LMG 958(T) (98.6%), S. rhizophila CCUG 47042(T) (98.3%), S. koreensis TR6-01(T) (97.6%) and S. acidaminiphila CIP 106456(T) (97.0%). Based on these results and modest DNA-DNA hybridization levels with S. maltophilia VKM B-591(T) (=LMG 958(T)) (51%) and S. rhizophila CCUG 47042(T) (52%), the isolate was classified as a novel species, Stenotrophomonas chelatiphaga sp. nov. (type strain LPM-5(T)=VKM B-2486=DSM-21508=CCUG 57178).
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS and Pushchino State University, Pushchino, Moscow Region 142290, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kaparullina
authors.forename: Nina
authors.initials: N
authors.lastname: Doronina
authors.forename: Tatyana
authors.initials: T
authors.lastname: Chistyakova
authors.forename: Yuri
authors.initials: Y
authors.lastname: Trotsenko
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004492:Edetic Acid / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D013045:Species Specificity; D020588:Stenotrophomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D004492:Edetic Acid