title: Apoptosis of interstitial cells of Cajal, smooth muscle cells, and enteric neurons induced by intestinal ischemia and reperfusion injury in adult guinea pigs.
abstract: This study aimed at evaluating whether apoptosis of interstitial cells of Cajal (ICC), smooth muscle cells (SMC), and enteric neurons was involved in a guinea pig model of intestinal ischemia and reperfusion injury. The small intestinal segments were resected at either 6 (I60/R6h) and 12 h (I60/R12h) or 7 (I60/R7d) to 14 (I60/R14d) days after 60 min intestinal ischemia in the adult guinea pigs and studied by immunohistochemistry with anti-Kit, 5-bromo-2'-deoxyuridine (BrdU), alpha-smooth muscle actin, vimentin, and beta-tublin III antibodies. Also, apoptosis was tested by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. In the I60/R12h injury, there was a approximately 50% decrease of Kit+ cells in cell numbers at the level of myenteric plexus and a number of Kit-/vimentin-positive cells were labeled by TUNEL. Also, a few SMC and enteric neurons were TUNEL positive. The Kit+ ICC recovered to normal and a number of Kit-/BrdU-double-positive cells were observed in the I60/R14d group. Our results indicated that the intestinal I/R injury could lead to apoptosis of ICC, SMC, and enteric neurons which may contribute to the gastrointestinal motility disorders, and proliferation was involved in the recovery of ICC.
authors.affiliation: Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. thethirdmilitary@sohu.com
authors.forename: Feng
authors.initials: F
authors.lastname: Mei
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.forename: Yang-tao
authors.initials: YT
authors.lastname: He
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: De-shan
authors.initials: DS
authors.lastname: Zhou
authors.forename: Jian-qin
authors.initials: JQ
authors.lastname: Niu
authors.forename: Han-zhi
authors.initials: HZ
authors.lastname: Wang
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Tian
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007410:Intestinal Diseases / Q000378:metabolism / Q000473:pathology*; D007422:Intestines / Q000098:blood supply / Q000294:innervation / Q000473:pathology; D009197:Myenteric Plexus / Q000378:metabolism / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D014404:Tubulin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D000199:Actins; D014404:Tubulin; D014746:Vimentin