The expression and concentration of CD40 ligand in normal pregnancy and pre-eclampsia.
This study investigated the surface expression of the CD154 antigen (CD40L) on peripheral blood CD4+ T lymphocytes and the sera concentrations of soluble CD40L antigens (sCD40L) in non-pregnant women, normal pregnant women, and patients with pre-eclampsia. Twenty-five patients with pre-eclampsia, 18 healthy pregnant women, and 10 healthy non-pregnant women were included in the study. The expression of the CD154 antigen on CD4+ T lymphocytes was determined using flow cytometry. The serum concentration of sCD40L was measured using an ELISA. Statistical analysis was performed using the Mann-Whitney U and ANOVA tests. The expression of the CD154 antigen on CD4+ T lymphocytes and the serum concentration of soluble CD40L were significantly higher in pre-eclampsia than in normal pregnant women. In normal pregnancy the expression of the CD154 antigen on CD4+ T lymphocytes and the concentration of sCD40L were significantly lower than in non-pregnant women. We conclude that during normal pregnancy the levels of these inflammatory mediators are lower than in non-pregnant women. In pre-eclampsia the levels are higher than those in normal pregnancy, but stable compared with the non-pregnant state. These results suggest that in pre-eclampsia, there are disturbances in the mechanisms responsible for the decrease in innate immunity which occurs in normal pregnancy.
Darmochwal-Kolarz
D
D
Department of Obstetrics and Perinatology, Medical University of Lublin, Lublin, Poland. dorotak@mp.pl
Kolarz
B
B
Surdacka
A
A
Rolinski
J
J
Leszczynska-Gorzelak
B
B
Oleszczuk
J
J
D023201:CD40 Ligand / Q000097:blood* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006262:Health; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D013601:T-Lymphocytes / Q000378:metabolism
D023201:CD40 Ligand