Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR).
Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only forthe identification of the 5 standard Vibrio sp simultaneously but also for the detection of Vibrio spin the samples collected from the natural environment.
Kim
Mal Nam
MN
Department of Biology, Sangmyung University, Seoul-110-743, Korea. mnkim@smu.ac.kr
Bang
Hyo Joo
HJ
D008386:Marine Biology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014733:Vibrio / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D014871:Water Microbiology
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S