Release and clinical significance of soluble CD83 in chronic lymphocytic leukemia.
Soluble CD83 (sCD83), a potent immunosuppressive agent, circulates at elevated levels in some chronic lymphocytic leukemia (CLL) patients. We report that CLL patients with elevated plasma sCD83 levels had significantly shorter (P=0.038) treatment free survival. Culture of CLL cells with solid phase CD83 mAb+IL-4 significantly increases sCD83 release (23-117-fold, P=0.013) and ligation of normal donor PBMC with solid phase CD83 mAb alone induces similar significant increases in sCD83 release (P=0.003). RT-PCR analysis detected the presence of a transcript for sCD83 in 2/3 CLL samples. These results suggest sCD83 release may play a regulatory role in CLL progression.
Hock
B D
BD
Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand. barry.hock@chmeds.ac.nz
Fernyhough
L J
LJ
Gough
S M
SM
Steinkasserer
A
A
Cox
A G
AG
McKenzie
J L
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015703:Antigens, CD / Q000097:blood* / Q000276:immunology; D002908:Chronic Disease; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood* / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000097:blood* / Q000188:drug therapy / Q000276:immunology / Q000401:mortality; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood* / Q000276:immunology; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000097:blood / Q000276:immunology; D012334:RNA, Neoplasm / Q000097:blood / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D000097802:CD83 Antigen
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007136:Immunoglobulins; D015847:Interleukin-4; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000097802:CD83 Antigen; C508598:IL4 protein, human