title: Porous bioactive scaffold of aliphatic polyurethane and hydroxyapatite for tissue regeneration.
abstract: In this study, a new hydroxyapatite (HA)/polyurethane (PU) composite porous scaffold was developed by in situ polymerization. Aliphatic isophorone diisocyanate as a nontoxic and safe agent was adopted to produce the rigid segment in polyurethane polymerization. Hydroxyapatite powder was compounded in a PU polymer matrix during the polymeric process. The macrostructure and morphology as well as mechanical strength of the scaffolds were characterized by FTIR, XRD, DSC and SEM. The results show that the isophorone diisocyanate can react mildly with hydroxyl (-OH) groups of castor oil and a mild foaming action caused by the release of CO2 gas occurred simultaneously in the reactive process, thus producing a uniform porous structure of HA/PU scaffold. The HA/PU composite scaffold with a high HA content of about 60 wt% has a porosity of more than 78% and a pore size from 100 microm to 800 microm. The HA/PU scaffold exhibited good cytocompatibility estimated by co-culturing the scaffold with MG63 cells through MTT test. The porous composite scaffold has good homogenization and a perfect three-dimensional structure for cell migration and bone tissue ingrowth, and should have good prospects for bone tissue regeneration.
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zou
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
mesh_terms: D002152:Calorimetry, Differential Scanning / Q000379:methods; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D017886:Durapatite; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D012516:Osteosarcoma / Q000473:pathology; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D011208:Powders; D012038:Regeneration; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011140:Polyurethanes; D011208:Powders; D013778:Tetrazolium Salts; D013844:Thiazoles; D017886:Durapatite; C022616:thiazolyl blue