Safety assessment and detection method of genetically modified Chinese Kale (Brassica oleracea cv. alboglabra ).
Sporamins are tuberous storage proteins and account for 80% of soluble protein in sweet potato tubers with trypsin-inhibitory activity. The expression of sporamin protein in transgenic Chinese kale (line BoA 3-1) conferred insecticidal activity toward corn earworm [ Helicoverpa armigera (Hubner)] in a previous report. In this study, we present a preliminary safety assessment of transgenic Chinese kale BoA 3-1. Bioinformatic and simulated gastric fluid (SGF) analyses were performed to evaluate the allergenicity of sporamin protein. The substantial equivalence between transgenic Chinese kale and its wild-type host has been demonstrated by the comparison of important constituents. A reliable real-time polymerase chain reaction (PCR) detection method was also developed to control sample quality. Despite the results of most evaluations in this study being negative, the safety of sporamin in transgenic Chinese kale BoA 3-1 was uncluded because of the allergenic risk revealed by bioinformatic analysis.
Lin
Chih-Hui
CH
Institute of Microbiology and Biochemistry, College of Life Science, National Taiwan University, Number 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
Lu
Chien-Te
CT
Lin
Hsin-Tang
HT
Pan
Tzu-Ming
TM
D001937:Brassica / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology; D003257:Consumer Product Safety; D005512:Food Hypersensitivity / Q000378:metabolism / Q000517:prevention & control; D005750:Gastric Juice / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009753:Nutritive Value; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction
D010940:Plant Proteins; C067142:sporamin protein, Ipomoea batatas