title: CABS1 is a novel calcium-binding protein specifically expressed in elongate spermatids of mice.
abstract: Single intraperitoneal injection of busulfan at 20 mg/kg body weight to mature male mice induced the deletion of the spermatogenic cells, followed by the restoration of the spermatogenesis by the surviving undifferentiated spermatogonia. The changes of the protein contents in testis during these processes were analyzed by two-dimensional gel electrophoresis in order to identify the proteins expressed at the specific stages of spermatogenesis. An acidic protein that disappeared and recovered in the same time course as spermatids after the busulfan treatment was identified as CABS1 by mass spectrometry. It was found that CABS1 was specifically expressed in the elongate spermatids at steps 13 to 16 in stages I to VIII of the seminiferous epithelium cycle of the mouse, and then it localized to the principal piece of flagellum of the mature sperm in the cauda epididymis. We have found for the first time that CABS1 is a calcium-binding protein that binds calcium during the maturation in the epididymis.
authors.affiliation: Graduate School of Science, University of Tokyo, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kawashima
authors.forename: Boran A H
authors.initials: BA
authors.lastname: Osman
authors.forename: Minoru
authors.initials: M
authors.lastname: Takashima
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Sae
authors.initials: S
authors.lastname: Kohchi
authors.forename: Emiko
authors.initials: E
authors.lastname: Satoh
authors.forename: Michiko
authors.initials: M
authors.lastname: Tamba
authors.forename: Manabu
authors.initials: M
authors.lastname: Matsuda
authors.forename: Naomichi
authors.initials: N
authors.lastname: Okamura
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating; D002066:Busulfan; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013082:Sperm Tail / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism*; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism
chemical_list: D018906:Antineoplastic Agents, Alkylating; C542345:CABS1 protein, mouse; D002135:Calcium-Binding Proteins; D002066:Busulfan; D002118:Calcium