Facile evaluation of cell disruption efficiency using pH-controlled fluorescence resonance energy transfer.
The evaluation of cell disruption efficiency is important in monitoring durability and diverse cellular physiologies of host cells and improving recovery yield of intracellular products. On the basis of our previously suggested pH-controlled fluorescence resonance energy transfer (FRET) system, we herein demonstrated that easy and effective estimation of the degree of cell disruption under certain stress environments is possible using a simple measurement of changes in fluorescence spectra of two fused fluorescent proteins, because its unique linear correlation was established with cell disruption efficiency from the conventional colony counting method.
Kim
Young Soo
YS
Dept of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
Cha
Hyung Joon
HJ
D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects; D031541:Fluorescence Resonance Energy Transfer; D006863:Hydrogen-Ion Concentration; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014465:Ultrasonics
D012967:Sodium Dodecyl Sulfate