Fabrication of F0F1-ATPase nanostructure on gold surface through Dip-Pen nanolithography.
DPN (Dip-Pen nanolithography) is one kind of widely used technique to create nanoscopic patterns of many different materials. FoF1-ATPase is nano scale rotary molecular motor, and it would be an ideal motor or energy providing device in micro/nano system. In this paper, we used DPN technique to create nanoarrays of F0F1-ATPase within chromatophore on gold surface. The feature size of our F0F1-ATPase patterns was 270 nm in average, and there were no more than 20 F0F1-ATPases in each dot. The activity of patterned F0F1-ATPase was demonstrated by its ATP synthesis, which was indicated by the fluorescence change of labeled F1300. The patterned F0F1-ATPase nanoarrays can be further used as biosensor, or power providing system. And precisely patterning F0F1-ATPase with desired size, position and biological activity will accelerate its application in many basic and application research fields.
Liu
Xiaolong
X
The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Yue
Jiachang
J
Yu
Gui
G
Liu
Zhongfan
Z
D000327:Adsorption; D003460:Crystallization / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000648:ultrastructure*; D013499:Surface Properties
D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D020409:Molecular Motor Proteins; D006046:Gold; D006180:Proton-Translocating ATPases