title: Investigations into the uptake of copper, iron and selenium by a highly sulphated bacterial exopolysaccharide isolated from microbial mats.
abstract: A bacterium isolated from microbial mats located on a polynesian atoll produced a high molecular weight (3,000 kDa) and highly sulphated exopolysaccharide. Previous studies showed that the chemical structure of this EPS consisted of neutral sugars, uronic acids, and high proportions of acetate and sulphate groups. The copper- and iron-binding ability of the purified pre-treated native EPS was investigated. Results showed that this EPS had a very high affinity for both copper (9.84 mmol g(-1) EPS) and ferrous iron (6.9 mmol g(-1) EPS). Amazingly, this EPS did not show any affinity for either ferric ions or selenium salts. This finding is one of the first steps in assessing the biotechnological potential of this polysaccharide.
authors.affiliation: CAIRAP Sarl, Centre d'Analyses Industrielles et de Recherche Appliquée pour le Pacifique, BP 1720, 98713, Papeete, Tahiti, French Polynesia. xmoppert@cairap.pf
authors.forename: Xavier
authors.initials: X
authors.lastname: Moppert
authors.forename: Tinaïg
authors.initials: T
authors.lastname: Le Costaouec
authors.forename: Gérard
authors.initials: G
authors.lastname: Raguenes
authors.forename: Anthony
authors.initials: A
authors.lastname: Courtois
authors.forename: Christelle
authors.initials: C
authors.lastname: Simon-Colin
authors.forename: Philippe
authors.initials: P
authors.lastname: Crassous
authors.forename: Bernard
authors.initials: B
authors.lastname: Costa
authors.forename: Jean
authors.initials: J
authors.lastname: Guezennec
mesh_terms: D000042:Absorption; D003300:Copper / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D010230:Paracoccus / Q000737:chemistry / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012643:Selenium / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D013431:Sulfates; D003300:Copper; D007501:Iron; D012643:Selenium