Engineering bisquinolinium/thiazole orange conjugates for fluorescent sensing of G-quadruplex DNA.
Lighting up: A G-quadruplex-specific fluorescent probe was designed combining the specificity of the pyridodicarboxamide motif for guanine quadruplexes and the fluorescence properties of thiazole orange. While the assembly of the two partners through a flexible linker leads to a nonselective probe, merging them in a single, rigid scaffold leads to a dye that elicits the properties required for G-quadruplex sensing.
Yang
Peng
P
Muséum National d'Histoire Naturelle, USM 503 INSERM, U565, CNRS, UMR5153, 43 Rue Cuvier, Case postale 26, 75005 Paris, France.
De Cian
Anne
A
Teulade-Fichou
Marie-Paule
MP
Mergny
Jean-Louis
JL
Monchaud
David
D
D052160:Benzothiazoles / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D011804:Quinolines / Q000737:chemistry*; D011806:Quinolinium Compounds / Q000737:chemistry
D052160:Benzothiazoles; D005456:Fluorescent Dyes; C540258:PDC-M-TO 2; D011804:Quinolines; D011806:Quinolinium Compounds; C051000:thiazole orange; D004247:DNA