Propolis and Herba Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker, Myxocyprinus asiaticus.
The effect of traditional Chinese medicine (TCM) formulated from propolis and Herba Epimedii extracts at the ratio of 3:1 (w/w) on non-specific immune response of Chinese sucker (Myxocyprinus asiaticus) was investigated. Fish were fed diets containing 0 (control), 0.1%, 0.5% or 1.0% TCM extracts for five weeks. The respiratory burst and phagocytic activities of blood leukocytes, lysozyme and natural haemolytic complement activities in plasma were measured weekly. After five weeks of feeding, fish were infected with Aeromonas hydrophila and mortalities were recorded. Results of this study showed that feeding Chinese sucker with different dosage of TCM extracts stimulated respiratory burst activity, phagocytosis of phagocytic cells in blood and lysozyme activity in plasma. They had no effect on plasma natural haemolytic complement activity. All dosage of treated groups showed reduced mortality following A. hydrophila infection. Feed containing 0.5% TCM extracts was the most effective with the mortality of the fish significantly reduced by 35% compared to the control. The results indicate that propolis and Herba Epimedii extracts in combination enhances the non-specific immune response and disease resistance of Chinese sucker against A. hydrophila.
Zhang
Guobin
G
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Gong
Shiyuan
S
Yu
Denghang
D
Yuan
Hanwen
H
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D003165:Complement System Proteins / Q000378:metabolism; D003531:Cypriniformes / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005393:Fish Diseases / Q000276:immunology* / Q000401:mortality; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D007113:Immunity, Innate / Q000187:drug effects*; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011429:Propolis / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C046314:epimedii flavone; D003165:Complement System Proteins; D011429:Propolis; D009113:Muramidase