Chiral separations of some beta-adrenergic agonists and antagonists on AmyCoat column by HPLC.
Sixteen beta-adrenergic antagonists namely acebutalol, alprenolol, atenolol, bisoprolol, bopindolol, bufurolol, carazolol, celiprolol, indenolol, metaprolol, nebivolol, oxprenolol, practolol, propranolol, tertalol, and timolol, and two beta-adrenergic agonists namely cimeterol and clenbuterol were resolved on AmyCoat (150 x 46 mm, 3 microm size of silica particle) by using (85:15:0.1, v/v/v), (90:10:0.1, v/v/v), and (95:05:0.1, v/v/v) combinations of n-heptane, ethanol, and diethylamine solvents, respectively. The flow rates used were 0.5, 1.0, 2.0, and 3.0 ml/min with detection at 225 nm. The values of capacity, separation, and resolution factors ranged from 0.38 to 19.70, 1.08-2.33, and 1.0 and 4.50, respectively. The maximum and minimum resolutions were achieved for celiprolol and bufurolol, respectively. The chiral recognition mechanisms were also discussed. The values of validation parameters were calculated.
Ali
Imran
I
Department of Chemistry Jamia Millia Islamia (Central University), New Delhi 110025, India.
Saleem
Kishwar
K
Gaitonde
Vinay D
VD
Aboul-Enein
Hassan Y
HY
Hussain
Iqbal
I
D000318:Adrenergic beta-Agonists / Q000737:chemistry / Q000302:isolation & purification*; D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists