Silk fibroin/polyacrylamide semi-interpenetrating network hydrogels for controlled drug release.
The present study describes a semi-interpenetrating network hydrogel fabricated using silk fibroin/polyacrylamide for controlled drug delivery applications. Hydrogels were synthesized using varied ratios of silk fibroin/acrylamide mixtures crosslinked by N,N'-Methylenebisacrylamide. Fourier-Transform Infrared analysis was performed suggesting beta sheet transition of silk fibroin with hydrogels. Scanning electron microscopy revealed microporous surface with maximum pore size of 50+/-11 microm. Rheological properties along with swellability, degradation, sol fraction estimation, equilibrium water content and swelling kinetics were evaluated. Compressive strength of 241.9+/-5.5 kPa was observed suggesting mechanically stronger gels. MTT assay showed biocompatibility and absence of deleterious effects of hydrogel on cell viability and functionality. In vitro release studies using two model compounds i.e. trypan blue dye and FITC-inulin reveal their sustained release from the fabricated hydrogel constructs.
Mandal
Biman B
BB
Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, West Bengal, India.
Kapoor
Sonia
S
Kundu
Subhas C
SC
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D012831:Bombyx / Q000378:metabolism; D002460:Cell Line; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D044367:Phase Transition; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001672:Biocompatible Materials; D005349:Fibroins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D004364:Pharmaceutical Preparations; D014867:Water; D000099166:Polyacrylamides