[Functional activity of the rat blood leucocytes during dynamics of nitric hypoxia].
Alert Wistar rats were subcutaneously injected with sodium nitrite (3 mg/100 g body weight), the control group rats were injected with saline. The blood was taken in 30, 60 and 90 minutes after the injection. Total leucocyte quantity and their subpopulations, the oxygen-dependent and oxygen-independent phagocytosis by neutrophils, functional activity of T-lymphocytes and their responsiveness to neurotransmitters and sodium nitrite and sodium nitrite and basal cell activity mobility were studied. It was shown that a more obvious alteration of the lymphocyte number, an increase of migratory and cytokine-productive activity, as well as augmentation of the cell sensitivity response to sodium nitrite and acetylcholine occurred in 30 minutes after the nitric oxide donor injection. At the same time, the neutrophil radical-productive activity and quantity of cationic peptides in neutrophils remained unchanged during nitric intoxication. At a later time, the leucocyte state did not change as compared with the control parameters.
Tsoĭ
E M
EM
Kovalenko
R I
RI
Nozdrachev
A D
AD
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D002465:Cell Movement / Q000187:drug effects*; D005520:Food Preservatives / Q000633:toxicity*; D000860:Hypoxia / Q000097:blood* / Q000139:chemically induced; D007962:Leukocytes / Q000378:metabolism*; D018655:Lymphocyte Count; D008297:Male; D018377:Neurotransmitter Agents / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012977:Sodium Nitrite / Q000633:toxicity*; D013997:Time Factors
D023181:Antimicrobial Cationic Peptides; D005520:Food Preservatives; D018377:Neurotransmitter Agents; D012977:Sodium Nitrite