Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal.
OBJECTIVE
Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa).


METHODS
Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n=150, age: 46.6+/-7.7 years), and female controls (n=150, age: 46.8+/-7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction.


RESULTS
Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001; 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N(2),N(2)-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients.


CONCLUSIONS
This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.
Cho
Sung-Hee
SH
Life Sciences Division, Korea Institute of Science and Technology, 39-1 Hawolkok-dong, Seoul, Korea.
Choi
Man Ho
MH
Lee
Won-Yong
WY
Chung
Bong Chul
BC
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000241:Adenosine / Q000031:analogs & derivatives / Q000652:urine; D000328:Adult; D001943:Breast Neoplasms / Q000601:surgery / Q000652:urine; D002853:Chromatography, Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008408:Mastectomy; D008875:Middle Aged; D009705:Nucleosides / Q000652:urine*; D018384:Oxidative Stress / Q000502:physiology; D011184:Postoperative Period; D017382:Reactive Oxygen Species / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013936:Thymidine / Q000031:analogs & derivatives / Q000652:urine
D009705:Nucleosides; D017382:Reactive Oxygen Species; D006151:Guanosine; C002230:1-methyladenosine; C008940:N(2),N(2)-dimethylguanosine; C018080:5-hydroxymethyl-2'-deoxyuridine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000241:Adenosine; D013936:Thymidine