Receptor arrays for the selective and efficient capturing of viral particles.
We describe microarrays of receptors on gold/glass substrates for the selective capturing of viral particles at high density. Microscale gold squares were surface-modified with alkanethiol derivatives which enabled the immobilization of the His(6)-tagged virus-binding domain from the very-low density lipoprotein (VLDL) receptor. The free glass areas surrounding the gold squares were passivated with a dense film of poly(ethylene glycol) (PEG). As assessed by atomic force microscopy, human rhinovirus particles were captured onto the VLDL-receptor patches with a high surface coverage but were effectively repelled by the PEG layer, resulting in a 330 000-fold higher density of the particles on the gold as compared to the glass surfaces. The metal chelate-based coupling strategy was found to be superior to two alternative routes, which used the covalent coupling of viral particles or viral receptors to the substrate surface. The high-density receptor arrays were employed for sensing and characterizing viral particles with so far unprecedented selectivity.
Pollheimer
Philipp D
PD
Institute of Biophysics, Johannes Kepler University, Linz, Austria.
Kastner
Markus
M
Ebner
Andreas
A
Blaas
Dieter
D
Hinterdorfer
Peter
P
Gruber
Hermann J
HJ
Howorka
Stefan
S
D005898:Glass / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008079:Lipoproteins, VLDL / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry; D011973:Receptors, LDL / Q000737:chemistry / Q000378:metabolism*; D012229:Rhinovirus / Q000302:isolation & purification* / Q000378:metabolism; D013499:Surface Properties; D014771:Virion / Q000302:isolation & purification* / Q000378:metabolism
D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; C036053:VLDL receptor; D011092:Polyethylene Glycols; D006046:Gold