Obstructive apneas induce early release of mesenchymal stem cells into circulating blood.
OBJECTIVE
To investigate whether noninvasive application of recurrent airway obstructions induces early release of mesenchymal stem cells into the circulating blood in a rat model of obstructive sleep apnea.


METHODS
Prospective controlled animal study.


METHODS
University laboratory.


METHODS
Twenty male Sprague-Dawley rats (250-300 g).


METHODS
A specially designed nasal mask was applied to the anesthetized rats. Ten rats were subjected to a pattern of recurrent obstructive apneas (60 per hour, lasting 15 seconds each) for 5 hours. Ten anesthetized rats were used as controls.


RESULTS
Mesenchymal stem cells from the blood and bone marrow samples were isolated and cultured to count the total number of colony-forming unit fibroblasts (CFU-F) of adherent cells after 9 days in culture. The number of CFU-F from circulating blood was significantly (P = 0.02) higher in the rats subjected to recurrent obstructive apneas (5.00 +/- 1.16; mean +/- SEM) than in controls (1.70 +/- 0.72). No significant (P = 0.54) differences were observed in CFU-F from bone marrow.


CONCLUSIONS
Application of a pattern of airway obstructions similar to those experienced by patients with sleep apnea induced an early mobilization of mesenchymal stem cells into circulating blood.
Carreras
Alba
A
Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona--IDIBAPS, Barcelona, Spain.
Almendros
Isaac
I
Acerbi
Irene
I
Montserrat
Josep M
JM
Navajas
Daniel
D
Farré
Ramon
R
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020181:Sleep Apnea, Obstructive / Q000097:blood*; D013234:Stem Cells / Q000166:cytology