title: Optimal immobilization of beta-galactosidase from Pea (PsBGAL) onto Sephadex and chitosan beads using response surface methodology and its applications.
abstract: Response surface methodology (RSM) and centre composite design (CCD) were used to optimize immobilization of beta-galactosidase (BGAL) from Pisum sativum onto two matrices: Sephadex G-75 and chitosan beads. The immobilization efficiency of 75.66% and 75.19% were achieved with Sephadex G-75 and chitosan, respectively. There was broad divergence in physico-chemical properties of Sephadex-PsBGAL and chitosan-PsBGAL. Chitosan-PsBGAL was better suited for industrial application based on its broad pH and temperature optima, higher temperature stability, reusability etc. Sephadex-PsBGAL and chitosan-PsBGAL showed much variation in their catalytic properties with respect to soluble enzyme. About 50% loss in activity of Sephadex-PsBGAL and chitosan-PsBGAL were observed after 12 and 46 days at 4 degrees C, respectively. Chitosan-PsBGAL showed higher rate of lactose hydrolysis present in milk and whey at room temperature and 4 degrees C than Sephadex-PsBGAL. In both cases, lactose of milk whey was hydrolyzed at higher rate than that of milk.
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Alka
authors.initials: A
authors.lastname: Dwevedi
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D008863:Microspheres; D018532:Pisum sativum / Q000201:enzymology*; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: D003911:Dextrans; D004800:Enzymes, Immobilized; D048271:Chitosan; C025614:sephadex; D001616:beta-Galactosidase