Antimicrobial activity of isolate HL-12 against Clavibacter michiganensis subsp. michiganensis in the presence of cadmium.
An actinomycete, strain HL-12, that was isolated from a farmland on the Huajiachi campus of Zhejiang University was capable of inhibiting the growth of Clavibacter michiganensis subsp. michiganensis (Cmm) and was identified as a member of Streptomyces. Its antimicrobial activity against Cmm was measured using the agar plate sensitivity method in pure culture and evaluated by the inhibition ratio of Cmm in soil. The inhibitory activity of strain HL-12 against Cmm following exposure to low concentrations of Cd was greater than the inhibitory activity following exposure to high concentrations of Cd both in liquid culture and in soil. A stronger inhibition was also seen following a 24 h preculture in the presence of Cd in liquid culture. The growth of Cmm in soil was stimulated at low concentrations of Cd (<5.0 mg Cd kg(-1) dry soil) but inhibited when cultured in high concentrations of Cd (5.0 and 10.0 mg Cd kg(-1) dry soil). A higher inhibition ratio of strain HL-12 against Cmm, which was over 40% after soil incubation for 2 weeks, was observed following exposure to low concentrations of Cd (<5.0 mg Cd kg(-1) dry soil).
Yuan
Hai-ping
HP
Institute of Microbiology, College of Life Sciences, Zhejiang University, Zhejiang, People's Republic of China.
Min
Hang
H
Lv
Zhen-mei
ZM
Li
Zi-mu
ZM
D000192:Actinomycetales / Q000187:drug effects* / Q000254:growth & development*; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*; D013302:Streptomyces / Q000235:genetics / Q000472:pathogenicity*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D002104:Cadmium