title: Metabolomic assessment of oocyte viability.
abstract: The aim of the current study was to evaluate whether near-infrared (NIR) spectroscopy-generated metabolomic data obtained from oocyte culture samples would correlate with nuclear maturity status and derived embryo development. A total of 412 oocyte culture samples were collected from 43 patient cycles. Metabolomic profiles of metaphase I and II oocytes were obtained by NIR spectroscopy and were significantly different from each other and from profiles of prophase I (germinal vesicle) oocytes (P +/- 0.001 at the 95% confidence interval). Additionally, NIR spectroscopic analysis of culture medium of oocytes that developed to grade A embryos on day 3 demonstrated significantly higher viability indices (0.62 +/- 0.23) than those that developed to grades C/D (0.42 +/- 0.26; P < 0.006); and on day 5 grade A (0.37 +/- 0.20) was also higher than grades C/D (0.14 +/- 0.21; P < 0.02). Metabolomic profiles of oocytes that resulted in pregnancy had higher viability indices (0.87 +/- 0.27) than those that did not (0.44 +/- 0.17; P < 0.0001). The results of the current study demonstrate that metabolomic profiling from spent culture medium of the oocyte is related to nuclear maturity, is able to predict embryo development at day 3 and day 5 stages, and relates to embryo viability.
authors.affiliation: Reproductive Biology Associates, Assisted Reproduction, 1150 Lake Hearn Drive, Suite 600, Atlanta, GA 30342, USA. peter.nagy@rba-online.com
authors.forename: Zsolt Peter
authors.initials: ZP
authors.lastname: Nagy
authors.forename: Stacey
authors.initials: S
authors.lastname: Jones-Colon
authors.forename: Pieter
authors.initials: P
authors.lastname: Roos
authors.forename: Lucy
authors.initials: L
authors.lastname: Botros
authors.forename: Ermanno
authors.initials: E
authors.lastname: Greco
authors.forename: Jennifer
authors.initials: J
authors.lastname: Dasig
authors.forename: Barry
authors.initials: B
authors.lastname: Behr
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D008297:Male; D055432:Metabolomics / Q000379:methods*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D011247:Pregnancy; D018873:Pregnancy Rate; D011786:Quality Control; D012107:Research Design; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods
chemical_list: D015415:Biomarkers; D003470:Culture Media