title: Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice.
abstract: Streptococcus pneumoniae is a bacterial microorganism that frequently causes serious infection, particularly in children and the elderly. Protection against infection with S. pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS), but the mechanisms responsible for the generation of anticapsular antibodies remain incompletely understood. The aim of the present study was to evaluate the role of CD1-restricted T cells in the antibody response to caps-PS. When immunized with Pneumo23, wild-type mice and CD1 knockout mice on BALB/c and C57BL/6 backgrounds generated immunoglobulin M (IgM) and IgG antibody responses to soluble caps-PS that were comparable. Similar results were obtained after immunization with heat-inactivated S. pneumoniae. The IgM and IgG antibody response of wild-type mice to Pneumo23 was not affected by an antagonizing monoclonal anti-CD1 antibody treatment. In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression.
authors.affiliation: University Hospital Leuven, Laboratory Medicine/Immunology, Campus Gasthuisberg, CDG 7th Floor, Herestraat 49, B-3000 Leuven, Belgium.
authors.forename: Leen
authors.initials: L
authors.lastname: Moens
authors.forename: Axel
authors.initials: A
authors.lastname: Jeurissen
authors.forename: Stefan
authors.initials: S
authors.lastname: Nierkens
authors.forename: Louis
authors.initials: L
authors.lastname: Boon
authors.forename: Luc
authors.initials: L
authors.lastname: Van Kaer
authors.forename: Ahmad
authors.initials: A
authors.lastname: Kasran
authors.forename: Greet
authors.initials: G
authors.lastname: Wuyts
authors.forename: Jan L
authors.initials: JL
authors.lastname: Ceuppens
authors.forename: Xavier
authors.initials: X
authors.lastname: Bossuyt
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D018949:Antigens, CD1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D022242:Pneumococcal Vaccines / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: C414006:23-valent pneumococcal capsular polysaccharide vaccine; D000907:Antibodies, Bacterial; D018949:Antigens, CD1; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D022242:Pneumococcal Vaccines