title: Neopyrrolomycins with broad spectrum antibacterial activity.
abstract: Three new antibiotics, neopyrrolomycins B (1), C (2), and D (3), with potent activity against Gram-positive pathogens were discovered. They exhibited MIC values < 1 microg/mL versus a number of resistant strains. The compounds were obtained from the ethyl acetate extracts of a Streptomyces sp. after purification by column chromatography and RP-HPLC. Their structures were elucidated using X-ray crystallography (1) and NMR spectroscopy (2 and 3).
authors.affiliation: AMRI, Bothell Research Center, 18804 North Creek Parkway, Bothell, Washington 98011, USA.
authors.forename: D Craig
authors.initials: DC
authors.lastname: Hopp
authors.forename: Joshua
authors.initials: J
authors.lastname: Rhea
authors.forename: Daniel
authors.initials: D
authors.lastname: Jacobsen
authors.forename: Khadidja
authors.initials: K
authors.lastname: Romari
authors.forename: Chris
authors.initials: C
authors.lastname: Smith
authors.forename: John
authors.initials: J
authors.lastname: Rabenstein
authors.forename: Macarena
authors.initials: M
authors.lastname: Irigoyen
authors.forename: Midori
authors.initials: M
authors.lastname: Clarke
authors.forename: Linda
authors.initials: L
authors.lastname: Francis
authors.forename: Michele
authors.initials: M
authors.lastname: Luche
authors.forename: Grant J
authors.initials: GJ
authors.lastname: Carr
authors.forename: Ulla
authors.initials: U
authors.lastname: Mocek
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D016984:Enterococcus faecium / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011758:Pyrroles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013296:Streptococcus pneumoniae / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D011758:Pyrroles; C550398:neopyrrolomycin B; C550399:neopyrrolomycin C; C066136:neopyrrolomycin; D014640:Vancomycin