title: [Low paraoxonase and arylesterase plasma activities in Mexican patients with coronary artery disease].
abstract: OBJECTIVE
  To determine the Paraoxonase-1 (PON1) activity as well as its pheno- and genotypes at position 192 in Mexican subjects with diagnosis of coronary heart disease (CHD).
  
  
  METHODS
  We determined the PON1-192 polymorphism by PCR-RFLP, and serum PON1 activity, using either paraoxon (PONase activity) or phenylacetate (ARE activity) as substrates, in 155 clinically healthy individuals (control group), and 155 patients with at least one myocardial infarction (CHD group). The biochemical A/B phenotype was determined by the ratio of the NaCI 1 M-stimulated PONase activity divided by the ARE activity.
  
  
  RESULTS
  We found significantly lower PONase and ARE activities in CHD patients as compared to controls (233.1 +/- 102.1 vs. 295.8 +/- 159.1 nmol/min/mL, and 103.1 +/- 33.7 vs 220.2 +/- 120.7 micromol/min/mL, respectively, p<0.05 for both). Allele and genotype frequencies for PON1-192 were similar in CHD patients and healthy controls. Moreover, in the control group, the PON1-192 Q/R genotype did not matched with the A/B phenotype as has been proposed by other studies.
  
  
  CONCLUSIONS
  There were important differences in the ARE and PONase activities between Mexican CHD patients and controls, suggesting that PON1 activity could be a good marker of CHD risk, whereas PON1-192 lacks of value to assess such risk.
authors.affiliation: Departamento de Fisiología, Instituto Nacional de Cardiología Ignacio Chávez, DF México.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gamboa
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Regalado
authors.forename: Claudia
authors.initials: C
authors.lastname: Huesca-Gómez
authors.forename: Carlos
authors.initials: C
authors.lastname: Posadas-Romero
authors.forename: Juan
authors.initials: J
authors.lastname: Verdejo Paris
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.forename: Oscar
authors.initials: O
authors.lastname: Pérez-Méndez
mesh_terms: D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000097:blood / Q000235:genetics; D008076:Cholesterol, HDL / Q000097:blood; D003324:Coronary Artery Disease / Q000201:enzymology*; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: D008076:Cholesterol, HDL; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase