[Analysis of genetic diversity of wild Rehmannia glutinosa by using RAPD and ISSR markers].
OBJECTIVE
To analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplified polymorphic DNA (RAPD) and inter sample sequence repeat (ISSR) for analysis of R. glutinosa accessions.


METHODS
Two molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions.


RESULTS
Average 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average (UPGMA) method.


CONCLUSIONS
A high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher genetic diversity of R. glutinosa germplasms than RAPD marker.
Wang
Yan
Y
College of Life Sciences, Capital Normal University, Beijing 100037, China.
Li
Xian-en
XE
Li
Xue-dong
XD
Qi
Jian-jun
JJ
Sun
Peng
P
Zhou
Li-li
LL
D014644:Genetic Variation / Q000235:genetics*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D032267:Rehmannia / Q000145:classification* / Q000235:genetics*