title: Structural basis of human high-density lipoprotein formation and assembly at sub nanometer resolution.
abstract: Human high-density lipoproteins (HDL) are protein/lipid particles of nanometer sizes. These nano particles are critical for transportation of the "bad cholesterol" from peripheral tissues back to the liver for clearance. An inverse correlation has been observed between the plasma HDL concentration and atherosclerosis. Furthermore, the HDL particle has also been utilized as a vehicle for drug delivery and for intracellular cell biology studies of membrane proteins. The structural basis of HDL formation and assembly, however, is poorly understood. Using high-resolution structural approaches, the formation and assembly of the HDL particle is being examined at atomic resolution, which is reviewed in this chapter. We will mainly focus on our own NMR studies of different apoAI conformations with a brief summary of previously published work by other laboratories.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48021, USA.
authors.forename: Arun
authors.initials: A
authors.lastname: Sivashanmugam
authors.forename: Yunhuang
authors.initials: Y
authors.lastname: Yang
authors.forename: Victoria
authors.initials: V
authors.lastname: Murray
authors.forename: Christopher
authors.initials: C
authors.lastname: McCullough
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Ren
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D053442:High-Density Lipoproteins, Pre-beta / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D008970:Molecular Weight; D036103:Nanotechnology / Q000379:methods*; D011487:Protein Conformation; D014461:Ultracentrifugation
chemical_list: D016632:Apolipoprotein A-I; D053442:High-Density Lipoproteins, Pre-beta; D008075:Lipoproteins, HDL