Kinetically controlled self-assembly of DNA oligomers.
Metastable two-stranded DNA loops can be assembled into extended DNA oligomers by kinetically controlled self-assembly. Along the designed reaction pathway, the sequence of hybridization reactions is controlled by progressively revealing toeholds required to initiate strand-displacement reactions. The product length depends inversely on seed concentration and ranges from a few hundred to several thousand base-pairs.
Lubrich
Daniel
D
Department of Physics, University of Oxford, South Parks Road, Oxford, OX1 3PU, UK. phyld@nus.edu.sg
Green
Simon J
SJ
Turberfield
Andrew J
AJ
D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D013816:Thermodynamics
D009841:Oligonucleotides; D004247:DNA