Horseradish peroxidase immobilized on poly(thiophene-2-aminophenol-3-thiopheneacetic acid) film electrode with Au nanoparticle-fabrication and evaluation as hydrogen peroxide sensor.
Enzyme immobilized electrode was fabricated by two methods. In one of the methods, gold-nanoparticles (Au-NPs) prepared by gamma-irradiation were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au-NPs loaded copolymer film through physical entrapment. In the other method, the Au-NPs was prepared by electrochemical reduction of Au ions on the surface of poly(Th-AP-TAA) and HRP was immobilized into the Au-NPs. The enzyme immobilized electrodes were tested for electrocatalytic activities towards sensing of H2O2.
Kim
Hwa-Jung
HJ
Department of Chemistry, BK21 NanoBiosensor Research Team, Hannam University, Daejeon 305-811, Republic of Korea.
Choi
Seong-Ho
SH
Oh
Sang-Hyub
SH
Woo
Jin-Chun
JC
Kim
Il Kwang
IK
D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011108:Polymers; D013876:Thiophenes; C538762:poly(thiophene-2-aminophenol-3-thiopheneacetic acid); D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase