Measurement of apparent cell radii using a multiple wave vector diffusion experiment.
It had been previously shown that an idealized version of the two-wave-vector extension of the NMR pulsed-field-gradient spin echo diffusion experiment can be used to determine the apparent radius of geometries with restricted diffusion. In the present work, the feasibility of the experiment was demonstrated in an NMR imaging experiment, in which the apparent radius of axons in white matter tissue was determined. Moreover, numerical simulations have been carried out to determine the reliability of the results. For small diffusion times, the radius is systematically underestimated. Larger gradient area, finite length gradient pulses, and a statistical distribution of radii within a voxel all have a minor influence on the estimated radius.
Weber
T
T
Department of Experimental Physics 5, Julius-Maximilians-Universität Würzburg, Am Hubland, Würzburg, Germany.
Ziener
C H
CH
Kampf
T
T
Herold
V
V
Bauer
W R
WR
Jakob
P M
PM
D000465:Algorithms; D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure*; D048429:Cell Size; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity