title: Proteomic analysis of Vibrio vulnificus M2799 grown under iron-repleted and iron-depleted conditions.
abstract: Vibrio vulnificus is an opportunistic marine bacterium that causes a serious, often fatal, infection in human. An important factor that determines the survival of V. vulnificus in the human body is the ability to acquire iron. The differential expression of proteins in whole-cell lysates of V. vulnificus M2799, a clinical isolate, was evaluated under iron-repleted and iron-depleted conditions during the early, mid and late logarithmic growth phases. A total of 32, 53 and 42 iron-regulated spots were detected by two-dimensional differential gel electrophoresis (2D-DIGE) in the early, mid and late logarithmic growth phases, respectively. Of these, 18 (early logarithmic growth phase), 31 (mid logarithmic growth phase) and 26 (late logarithmic growth phase) proteins were subsequently identified by matrix-assisted laser desorption/ionization-time of flight analysis. These proteins were classified into 10 functional categories, including inorganic ion transport and metabolism, carbohydrate transport and metabolism, and amino acid transport and metabolism. Based on this classification, the expression of proteins involved in the iron acquisition system increased from the early to the mid logarithmic growth phases, while that of proteins involved in other metabolic pathways increased from the mid to the late logarithmic growth phases. Furthermore, when the protein expression profile of the wild type bacterium was compared with that of the fur mutant grown under the iron-repleted condition, the expression of 18 proteins was found to be regulated by iron and Fur.
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Katsushiro
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kosakai
authors.forename: Satomi
authors.initials: S
authors.lastname: Ikebayashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujibo
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006801:Humans; D007501:Iron / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D041261:Vibrio vulnificus / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D007501:Iron