Calcium influx-dependent differential actions of superoxide and hydrogen peroxide on microvessel permeability.
Our previous study demonstrated that reactive oxygen species (ROS) released from activated blood cells contribute significantly to the increased microvessel permeability during inflammation. This study aims to define the individual roles of hydrogen peroxide (H(2)O(2)) and superoxide in ROS-induced increases in permeability and endothelial intracellular Ca(2+) concentration ([Ca(2+)](i)) in individually perfused rat mesenteric venules. Microvessel permeability was determined by measuring hydraulic conductivity (L(p)). Endothelial [Ca(2+)](i) was measured in fura-2 AM-loaded microvessels. Perfusing microvessels with superoxide generated by hypoxanthine and xanthine oxidase (HX/XO) induced immediate and transient increases in L(p). The mean peak value, which occurred within 5 min of HX/XO exposure, was 4.3 +/- 0.6 times that of the control. In contrast, the perfusion of H(2)O(2) (100 and 500 microM) caused no immediate increases in L(p). A significant L(p) increase, 3.6 +/- 0.6 times the control value, occurred 30 min after the perfusion of H(2)O(2) at 500 microM. The perfusion of H(2)O(2) at 100 or 500 microM for 1 h increased L(p) to 6.6 +/- 0.9 and 11.3 +/- 3.6 times the control value, respectively. The increased endothelial [Ca(2+)](i) in HX/XO or H(2)O(2) perfused vessels was correlated with the time course of the increases in L(p). Inhibiting Ca(2+) influx by LaCl(3) prevented the permeability increase induced by HX/XO or H(2)O(2). These results demonstrated differential actions of superoxide and H(2)O(2) on microvessel permeability and endothelial [Ca(2+)](i). Superoxide-induced permeability increases were immediate and transient, whereas H(2)O(2)-induced permeability increases were progressive, demonstrating concentration and time dependence. Ca(2+) influx plays an essential role in both superoxide and H(2)O(2)-induced permeability increases.
Zhou
Xueping
X
Dept. of Physiology and Pharmacology, School of Medicine, West Virginia Univ., Morgantown, WV 26506-9229, USA.
Wen
Ke
K
Yuan
Dong
D
Ai
Ling
L
He
Pingnian
P
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism*; D019271:Hypoxanthine / Q000378:metabolism; D008643:Mesentery / Q000098:blood supply; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D013997:Time Factors; D014969:Xanthine Oxidase / Q000378:metabolism
D017382:Reactive Oxygen Species; D013481:Superoxides; D019271:Hypoxanthine; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D002118:Calcium