title: 2-Aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline.
abstract: 2-Aminopurine-modified abasic-site-containing duplex [DNA 5'-TCTGC GTCCT PXT TAACG CACAC-3'/3'-AGACG CAGGA TCA ATTGC GTGTG-5'; P = 2-aminopurine, X = abasic site (Spacer-C3), C = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 microM (5 degrees C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Minjie
authors.initials: M
authors.lastname: Li
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Takehiro
authors.initials: T
authors.lastname: Seino
authors.forename: Kodai
authors.initials: K
authors.lastname: Nakamura
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
mesh_terms: D015075:2-Aminopurine / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013806:Theophylline / Q000032:analysis* / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D005456:Fluorescent Dyes; D015075:2-Aminopurine; D004247:DNA; D013806:Theophylline