Cloning, expression and immunogenicity of the avian pneumovirus (Colorado isolate) F protein.
The F protein of the Colorado isolate of avian pneumovirus (APV), expressed from a DNA plasmid, was recognized by antiserum to both A and B subgroup APVs. After two intramuscular injections of turkeys with this plasmid, a homologous antibody response was detected by enzyme-linked immunosorbent assay. This antibody also recognized subgroup A APV. However, there was no neutralization of the Colorado isolate or of subgroup A or B viruses. Although no significant clinical protection was detected following homologous challenge of poults, an anamnestic serological response was seen, suggesting that a systemic antibody response but no local mucosal immunity was induced.
Tarpey
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I
Huggins
M B
MB
Davis
P J
PJ
Shilleto
R
R
Orbell
S J
SJ
Cook
J K
JK