title: Bactericidal efficacy of nitric oxide-releasing silica nanoparticles.
abstract: The utility of nitric oxide (NO)-releasing silica nanoparticles as novel antibacterial agents is demonstrated against Pseudomonas aeruginosa. Nitric oxide-releasing nanoparticles were prepared via co-condensation of tetraalkoxysilane with aminoalkoxysilane modified with diazeniumdiolate NO donors, allowing for the storage of large NO payloads. Comparison of the bactericidal efficacy of the NO-releasing nanoparticles to 1-[2-(carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolate (PROLI/NO), a small molecule NO donor, demonstrated enhanced bactericidal efficacy of nanoparticle-derived NO and reduced cytotoxicity to healthy cells (mammalian fibroblasts). Confocal microscopy revealed that fluorescently labeled NO-releasing nanoparticles associated with the bacterial cells, providing rationale for the enhanced bactericidal efficacy of the nanoparticles. Intracellular NO concentrations were measurable when the NO was delivered from nanoparticles as opposed to PROLI/NO. Collectively, these results demonstrate the advantage of delivering NO via nanoparticles for antimicrobial applications.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Evan M
authors.initials: EM
authors.lastname: Hetrick
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Stasko
authors.forename: C Bryce
authors.initials: CB
authors.lastname: Johnson
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Wespe
authors.forename: Ekhson
authors.initials: E
authors.lastname: Holmuhamedov
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D009569:Nitric Oxide; D012822:Silicon Dioxide