title: [Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD].
abstract: OBJECTIVE
  To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.
  
  
  METHODS
  HPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them.
  
  
  RESULTS
  Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol x L(-1) HCl, and furthermore their remained percentage was up to 98% and 103%.
  
  
  CONCLUSIONS
  The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol x L(-1) HCl in the solution.
authors.affiliation: School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Wan-jing
authors.initials: WJ
authors.lastname: Sun
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000097:blood* / Q000493:pharmacokinetics*; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D044950:Flavanones; D005419:Flavonoids; C038044:baicalin; C006680:baicalein