title: Horseradish peroxidase immobilized on poly(thiophene-2-aminophenol-3-thiopheneacetic acid) film electrode with Au nanoparticle-fabrication and evaluation as hydrogen peroxide sensor.
abstract: Enzyme immobilized electrode was fabricated by two methods. In one of the methods, gold-nanoparticles (Au-NPs) prepared by gamma-irradiation were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au-NPs loaded copolymer film through physical entrapment. In the other method, the Au-NPs was prepared by electrochemical reduction of Au ions on the surface of poly(Th-AP-TAA) and HRP was immobilized into the Au-NPs. The enzyme immobilized electrodes were tested for electrocatalytic activities towards sensing of H2O2.
authors.affiliation: Department of Chemistry, BK21 NanoBiosensor Research Team, Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Sang-Hyub
authors.initials: SH
authors.lastname: Oh
authors.forename: Jin-Chun
authors.initials: JC
authors.lastname: Woo
authors.forename: Il Kwang
authors.initials: IK
authors.lastname: Kim
mesh_terms: D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D013876:Thiophenes; C538762:poly(thiophene-2-aminophenol-3-thiopheneacetic acid); D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase