title: Flavonoids inhibit the AU-rich element binding of HuC.
abstract: Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC(50) values were low (0.2-1.8 microM). [BMB reports 2009; 42(1): 41-46].
authors.affiliation: Departments of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University, College of Medicine, Seoul 110-799, Korea.
authors.forename: Hojoong
authors.initials: H
authors.lastname: Kwak
authors.forename: Kyung-Chae
authors.initials: KC
authors.lastname: Jeong
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Chae
authors.forename: Soo-Youl
authors.initials: SY
authors.lastname: Kim
authors.forename: Woong-Yang
authors.initials: WY
authors.lastname: Park
mesh_terms: D001483:Base Sequence; D051959:ELAV Proteins / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics*; D013698:Templates, Genetic
chemical_list: D051959:ELAV Proteins; D005419:Flavonoids; D038621:Regulatory Sequences, Ribonucleic Acid