title: [Preliminary structural and functional study on a novel gene HSPCSET].
abstract: OBJECTIVE
  To characterize the structural and the functional feature of a novel gene HSPCSET isolated from human CD34+ hematopoietic stem/progenitor cells (HS/PCs).
  
  
  METHODS
  Bioinformatic technology was used to identify the structural features of the HSPCSET protein and perform the multiple sequence alignment. Yeast-two-hybrid system was used to identify the proteins interacting with the HSPCSET protein. After sequencing, we selected out the positive clones which had clear functions, and carried out beta-gal experiment and GST pull down assay to confirm the results. The cellular location of the HSPCSET was checked by immunofluorescence assay.
  
  
  RESULTS
  The HSPCSET protein belongs to a SET domain family, which is evolutionarily conserved across species. It implied that HSPCSET may have biologically important function. Using yeast-two-hybrid system, we showed that the protein sequence with SET domain might bind to 13 proteins, which involved in signaling transduction, transcriptional regulation, apoptosis, tumorigenesis, development, etc. And 4 proteins (GADD34, SIVA, DNAJ and PHF1) were confirmed by one-on-one back of the hybrid experiment, beta-gal test and GST pull down assay. When GADD34 and HSPCSET were co-transfected, they co-localized in the nucleus, suggesting a strong interaction.
  
  
  CONCLUSIONS
  The novel gene HSPCSET is likely to have biologically important function. This study provides the basis for further studies of its function in hematopoiesis and tumorigenesis.
authors.affiliation: Department of Hematology, Shanghai First People's Hospital, Shanghai, 200080 PR China.
authors.forename: Ju
authors.initials: J
authors.lastname: Wei
authors.forename: Xiao-jian
authors.initials: XJ
authors.lastname: Sun
authors.forename: Xin-yan
authors.initials: XY
authors.lastname: Wu
authors.forename: Sai-juan
authors.initials: SJ
authors.lastname: Chen
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Qiu-hua
authors.initials: QH
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D019295:Computational Biology; D017124:Conserved Sequence; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D054645:Protein Phosphatase 1; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
chemical_list: D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D011506:Proteins; C087519:PPP1R15A protein, human; D054645:Protein Phosphatase 1