HTLV-2B Tax oncoprotein is modified by ubiquitination and sumoylation and displays intracellular localization similar to its homologue HTLV-1 Tax.
HTLV-1 is more pathogenic than HTLV-2B. The difference is generally attributed to the properties of their individual transactivating Tax proteins. By using internal Flag-6His tagged Tax-1 and Tax-2B, which display transcriptional activities comparable to the untagged proteins and can be recognized by a single anti-Flag antibody, we demonstrate that Tax-2B is modified by ubiquitination and sumoylation. In addition, Tax2B is distributed in punctuate nuclear structures that include the RelA subunit of NF-kappaB, as has been previously demonstrated for Tax-1.
Turci
Marco
M
Department of Mother and Child, Biology and Genetics, Section of Biology and Genetics, University of Verona, Strada Le Grazie 8, I-37134 Verona, Italy.
Lodewick
Julie
J
Righi
Paola
P
Polania
Angela
A
Romanelli
Maria Grazia
MG
Bex
Françoise
F
Bertazzoni
Umberto
U
D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D016356:Gene Products, tax / Q000378:metabolism*; D015367:Human T-lymphotropic virus 2 / Q000502:physiology*; D006801:Humans; D051996:Transcription Factor RelA / Q000378:metabolism; D054875:Ubiquitination
D016356:Gene Products, tax; C498143:RELA protein, human; D051996:Transcription Factor RelA; C514971:tax protein, Human T-lymphotrophic virus 2