title: Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata.
abstract: The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis oculata.
authors.affiliation: Department of Biotechnology, Soonchunhyang University, Asan, Chungnan-336-600, Korea.
authors.forename: Joong Kee
authors.initials: JK
authors.lastname: Min
authors.forename: Tae Yeon
authors.initials: TY
authors.lastname: Rhim
authors.forename: Hyun Woong
authors.initials: HW
authors.lastname: Shin
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure; D056890:Eukaryota / Q000187:drug effects* / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D017931:DNA Primers; D002104:Cadmium; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase