title: Differentially expressed genes in eutopic and ectopic endometrium of women with endometriosis.
abstract: OBJECTIVE
  To elucidate the potential mechanisms involved in the physiopathology of endometriosis. We analyzed the differential gene expression profiles of eutopic and ectopic tissues from women with endometriosis.
  
  
  METHODS
  Prospective laboratory study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Seventeen patients in whom endometriosis was diagnosed and 11 healthy fertile women.
  
  
  METHODS
  Endometrial biopsy specimens from the endometrium of healthy women without endometriosis and from the eutopic and ectopic endometrium tissues of patients with endometriosis were obtained in the early proliferative phase of the menstrual cycle.
  
  
  METHODS
  Six paired samples of eutopic and ectopic tissue were analyzed by subtractive hybridization. To evaluate the expression of genes found by rapid subtraction hybridization methods, we measured CTGF, SPARC, MYC, MMP, and IGFBP1 genes by real-time polymerase chain reaction in all samples.
  
  
  RESULTS
  This study identified 291 deregulated genes in the endometriotic lesions. Significant expression differences were obtained for SPARC, MYC, and IGFBP1 in the peritoneal lesions and for MMP3 in the ovarian endometriomas. Additionally, significant differences were obtained for SPARC and IGFBP1 between the peritoneal and ovarian lesions. No significant differences were found for the studied genes between the control and the eutopic endometrium.
  
  
  CONCLUSIONS
  This study identified 291 genes with differential expression in endometriotic lesions. The deregulation of the SPARC, MYC, MMP3, and IGFBPI genes may be responsible for the loss of cellular homeostasis in endometriotic lesions.
authors.affiliation: Department of Genetics, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. jumeola@yahoo.com.br
authors.forename: Juliana
authors.initials: J
authors.lastname: Meola
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Rosa e Silva
authors.forename: Daniel Blassioli
authors.initials: DB
authors.lastname: Dentillo
authors.forename: Wilson Araújo
authors.initials: WA
authors.lastname: da Silva
authors.forename: Luciana Caricati
authors.initials: LC
authors.lastname: Veiga-Castelli
authors.forename: Luciano Angelo de Souza
authors.initials: LA
authors.lastname: Bernardes
authors.forename: Rui Alberto
authors.initials: RA
authors.lastname: Ferriani
authors.forename: Cláudia Cristina Paro
authors.initials: CC
authors.lastname: de Paz
authors.forename: Silvana
authors.initials: S
authors.lastname: Giuliatti
authors.forename: Lúcia
authors.initials: L
authors.lastname: Martelli
mesh_terms: D000293:Adolescent; D000328:Adult; D000465:Algorithms; D002828:Choristoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D016259:Genes, myc; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D010532:Peritoneal Diseases / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: C526317:CCN2 protein, human; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D015676:Osteonectin; D055513:Connective Tissue Growth Factor; D019278:Matrix Metalloproteinase 3