Carbohydrate dependent targeting of cancer cells by bleomycin-microbubble conjugates.
Biotinylated bleomycin A(5) was attached to streptavidin-derivatized microbubbles, and a solution containing the conjugate was passed over a monolayer of cultured MCF-7 cells. The bleomycin-derivatized microbubbles adhered to the MCF-7 cells, and the association could be monitored by the use of a microscope. Three other cancer cell lines gave similar results. The bleomycin-microbubble conjugate did not bind to a normal breast cell line (MCF-10A) or to the matched noncancer cell lines corresponding to the other cancer cell lines targeted by bleomycin. No binding to any tested cell line was observed when the microbubbles lacked conjugated bleomycin A(5) or when the microbubble contained a bleomycin A(5) analogue lacking the carbohydrate moiety.
Chapuis
Jean-Charles
JC
Center for BioEnergetics and Department of Chemistry, Arizona State University, Tempe, Arizona 85287, USA.
Schmaltz
Ryan M
RM
Tsosie
Krystal S
KS
Belohlavek
Marek
M
Hecht
Sidney M
SM
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D001761:Bleomycin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D045423:Microbubbles; D019809:Streptavidin / Q000008:administration & dosage / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C025703:bleomycetin; D001710:Biotin; D019809:Streptavidin