Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. coli methionine repressor, MetJ, for its operator DNA.
The efficient synthesis of a range of stable SAM mimetics, and their ability to promote the binding of the E. coli methionine repressor (MetJ) to its operator DNA, is described.
Joce
Catherine
C
School of Chemistry, University of Leeds, Leeds, UKLS2 9JT, UK.
Caryl
Jamie
J
Stockley
Peter G
PG
Warriner
Stuart
S
Nelson
Adam
A
D001426:Bacterial Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008024:Ligands; D011485:Protein Binding / Q000187:drug effects; D012097:Repressor Proteins / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000031:analogs & derivatives* / Q000138:chemical synthesis
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008024:Ligands; D012097:Repressor Proteins; C060486:methionine repressor protein, Bacteria; D012436:S-Adenosylmethionine; D004247:DNA