Complete sequence of biosynthetic gene cluster responsible for producing triostin A and evaluation of quinomycin-type antibiotics from Streptomyces triostinicus.
Streptomyces triostinicus produces triostin A, an antitumor antibiotic, as its major secondary metabolite. Surprisingly, this strain also produced a trace amount of echinomycin. We sequenced the entire triostin A biosynthetic gene cluster from S. triostinicus, and found that this 36 kilobase-long gene cluster contained an ORF homologous to ecm18 that encodes a thioacetal-forming enzyme responsible for the triostin A-to-echinomycin bioconversion. These findings indicate that, unlike previously thought, S. triostinicus is capable of producing not only triostin A but also echinomycin. Our observation suggests potential value in careful re-analysis for metabolites from previously characterized natural product producers with the current technologies.
Praseuth
Alex P
AP
Dept. Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA.
Wang
Clay C C
CC
Watanabe
Kenji
K
Hotta
Kinya
K
Oguri
Hiroki
H
Oikawa
Hideaki
H
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001483:Base Sequence; D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D004448:Echinomycin / Q000096:biosynthesis* / Q000737:chemistry; D015698:Genomic Library; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011810:Quinoxalines / Q000737:chemistry / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D011810:Quinoxalines; C031945:triostin A; D004448:Echinomycin