A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast.
A mutated allele of the essential gene TAH18 was previously identified in our laboratory in a genetic screen for new proteins interacting with the DNA polymerase delta in yeast [1]. The present work shows that Tah18 plays a role in response to oxidative stress. After exposure to lethal doses of H(2)O(2), GFP-Tah18 relocalizes to the mitochondria and controls mitochondria integrity and cell death. Dre2, an essential Fe/S cluster protein and homologue of human anti-apoptotic Ciapin1, was identified as a molecular partner of Tah18 in the absence of stress. Moreover, Ciapin1 is able to replace yeast Dre2 in vivo and physically interacts with Tah18. Our results are in favour of an oxidative stress-induced cell death in yeast that involves mitochondria and is controlled by the newly identified Dre2-Tah18 complex.
Vernis
Laurence
L
Régulation de la Réplication de l'ADN des Eucaryotes, UMR2027 CNRS/Institut Curie, INSERM, Université Paris-Sud, Orsay, France. laurence.vernis-beringue@curie.u-psud.fr
Facca
Céline
C
Delagoutte
Emmanuelle
E
Soler
Nicolas
N
Chanet
Roland
R
Guiard
Bernard
B
Faye
Gérard
G
Baldacci
Giuseppe
G
D000595:Amino Acid Sequence; D017353:Gene Deletion; D018628:Gene Dosage / Q000187:drug effects; D016153:Genes, Suppressor; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D050505:Mutant Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
C489114:CIAPIN1 protein, human; C531900:Dre2 protein, S cerevisiae; D047908:Intracellular Signaling Peptides and Proteins; D007506:Iron-Sulfur Proteins; D009153:Mutagens; D050505:Mutant Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide