title: MicroRNA transcriptome profiles during swine skeletal muscle development.
abstract: BACKGROUND
  MicroRNA (miR) are a class of small RNAs that regulate gene expression by inhibiting translation of protein encoding transcripts. To evaluate the role of miR in skeletal muscle of swine, global microRNA abundance was measured at specific developmental stages including proliferating satellite cells, three stages of fetal growth, day-old neonate, and the adult.
  
  
  RESULTS
  Twelve potential novel miR were detected that did not match previously reported sequences. In addition, a number of miR previously reported to be expressed in mammalian muscle were detected, having a variety of abundance patterns through muscle development. Muscle-specific miR-206 was nearly absent in proliferating satellite cells in culture, but was the highest abundant miR at other time points evaluated. In addition, miR-1 was moderately abundant throughout developmental stages with highest abundance in the adult. In contrast, miR-133 was moderately abundant in adult muscle and either not detectable or lowly abundant throughout fetal and neonate development. Changes in abundance of ubiquitously expressed miR were also observed. MiR-432 abundance was highest at the earliest stage of fetal development tested (60 day-old fetus) and decreased throughout development to the adult. Conversely, miR-24 and miR-27 exhibited greatest abundance in proliferating satellite cells and the adult, while abundance of miR-368, miR-376, and miR-423-5p was greatest in the neonate.
  
  
  CONCLUSIONS
  These data present a complete set of transcriptome profiles to evaluate miR abundance at specific stages of skeletal muscle growth in swine. Identification of these miR provides an initial group of miR that may play a vital role in muscle development and growth.
authors.affiliation: USDA/ARS Meat Animal Research Center, Clay Center, NE, USA. tara.mcdaneld@ars.usda.gov
authors.forename: Tara G
authors.initials: TG
authors.lastname: McDaneld
authors.forename: Timothy P L
authors.initials: TP
authors.lastname: Smith
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Doumit
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Miles
authors.forename: Luiz L
authors.initials: LL
authors.lastname: Coutinho
authors.forename: Tad S
authors.initials: TS
authors.lastname: Sonstegard
authors.forename: Lakshmi K
authors.initials: LK
authors.lastname: Matukumalli
authors.forename: Dan J
authors.initials: DJ
authors.lastname: Nonneman
authors.forename: Ralph T
authors.initials: RT
authors.lastname: Wiedmann
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D013552:Swine / Q000235:genetics* / Q000254:growth & development
chemical_list: D035683:MicroRNAs