title: p38 MAPK contributes to CD54 expression and the enhancement of phagocytic activity during macrophage development.
abstract: p38 is a subfamily of the mitogen-activated protein kinase (MAPK) superfamily with four isoforms. It has been well established that p38 plays a central role in the production of inflammatory molecules and is therefore required for the activation of macrophages in response to inflammatory stimuli. However, little is known about the roles of p38 in macrophage development. The difficulty to get mice deficient in multiple p38 isoforms complicates the study of p38 in macrophage development. With the model of bone marrow-derived murine macrophages and highly selective p38alpha/beta inhibitors SB203580 and SB239063, here we report that macrophage colony-stimulating factor (M-CSF) induces p38 activation during macrophage development. Inhibition of p38 activity showed minor effects on macrophage proliferation or survival, and did not block CD14, F4/80 expression. However, p38 inhibitors resulted in a significant reduction in CD54 expression and impaired phagocytic activity. Taken together, our data suggest that p38 contributes to macrophage development.
authors.affiliation: Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Jiannan
authors.initials: J
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jiyan
authors.initials: J
authors.lastname: Zhang
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D048290:Mitogen-Activated Protein Kinase 11 / Q000037:antagonists & inhibitors / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology* / Q000502:physiology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation; D039481:CD11b Antigen; D007093:Imidazoles; D018950:Lipopolysaccharide Receptors; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D011743:Pyrimidines; C036763:monocyte-macrophage differentiation antigen; D018799:Intercellular Adhesion Molecule-1; D016173:Macrophage Colony-Stimulating Factor; D048290:Mitogen-Activated Protein Kinase 11; D048051:p38 Mitogen-Activated Protein Kinases; C406525:SB 239063; C093642:SB 203580