title: Computational prediction of novel non-coding RNAs in Arabidopsis thaliana.
abstract: BACKGROUND
  Non-coding RNA (ncRNA) genes do not encode proteins but produce functional RNA molecules that play crucial roles in many key biological processes. Recent genome-wide transcriptional profiling studies using tiling arrays in organisms such as human and Arabidopsis have revealed a great number of transcripts, a large portion of which have little or no capability to encode proteins. This unexpected finding suggests that the currently known repertoire of ncRNAs may only represent a small fraction of ncRNAs of the organisms. Thus, efficient and effective prediction of ncRNAs has become an important task in bioinformatics in recent years. Among the available computational methods, the comparative genomic approach seems to be the most powerful to detect ncRNAs. The recent completion of the sequencing of several major plant genomes has made the approach possible for plants.
  
  
  RESULTS
  We have developed a pipeline to predict novel ncRNAs in the Arabidopsis (Arabidopsis thaliana) genome. It starts by comparing the expressed intergenic regions of Arabidopsis as provided in two whole-genome high-density oligo-probe arrays from the literature with the intergenic nucleotide sequences of all completely sequenced plant genomes including rice (Oryza sativa), poplar (Populus trichocarpa), grape (Vitis vinifera), and papaya (Carica papaya). By using multiple sequence alignment, a popular ncRNA prediction program (RNAz), wet-bench experimental validation, protein-coding potential analysis, and stringent screening against various ncRNA databases, the pipeline resulted in 16 families of novel ncRNAs (with a total of 21 ncRNAs).
  
  
  CONCLUSIONS
  In this paper, we undertake a genome-wide search for novel ncRNAs in the genome of Arabidopsis by a comparative genomics approach. The identified novel ncRNAs are evolutionarily conserved between Arabidopsis and other recently sequenced plants, and may conduct interesting novel biological functions.
authors.affiliation: Department of Computer Science and Technology, Tsinghua University, Beijing 100084, PR China. sdd00@mails.tsinghua.edu.cn
authors.forename: Dandan
authors.initials: D
authors.lastname: Song
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Binglian
authors.initials: B
authors.lastname: Zheng
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Deng
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Lu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D017124:Conserved Sequence; D030541:Databases, Genetic; D018745:Genome, Plant; D022661:RNA, Untranslated / Q000235:genetics*; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA
chemical_list: D029681:Arabidopsis Proteins; D022661:RNA, Untranslated