title: Replication of calpain-10 genetic association with carotid intima-media thickness.
abstract: OBJECTIVE
  Diabetes and atherosclerosis may share common genetic determinants. A prior study in Hispanics found association of haplotypes in the diabetes gene calpain-10 (CAPN10) with carotid artery intima-media thickness (CIMT). This study sought to replicate this association in an independent cohort.
  
  
  METHODS
  Four CAPN10 SNPs were genotyped and haplotypes determined in 487 Hispanic Americans from 143 families ascertained via an index case with hypertension. CIMT was measured from B-mode ultrasound, and glycemic traits quantified from euglycemic clamps. Association of SNPs and haplotypes with CIMT was determined.
  
  
  RESULTS
  The minor alleles of SNP-56 and SNP-63 were associated with increased CIMT in dominant and additive models. The association of haplotype 1112 with increased CIMT was replicated. No associations with fasting insulin, insulin secretion, or insulin sensitivity were observed.
  
  
  CONCLUSIONS
  CAPN10 association with CIMT was replicated, further supporting its role as a common genetic determinant of diabetes and atherosclerosis in Hispanics.
authors.affiliation: Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA. mark.goodarzi@cshs.org
authors.forename: Mark O
authors.initials: MO
authors.lastname: Goodarzi
authors.forename: Kent D
authors.initials: KD
authors.lastname: Taylor
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Jones
authors.forename: Belle
authors.initials: B
authors.lastname: Fang
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Guo
authors.forename: Anny H
authors.initials: AH
authors.lastname: Xiang
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Buchanan
authors.forename: Howard N
authors.initials: HN
authors.lastname: Hodis
authors.forename: Leslie J
authors.initials: LJ
authors.lastname: Raffel
authors.forename: Jerome I
authors.initials: JI
authors.lastname: Rotter
mesh_terms: D000328:Adult; D002154:Calpain / Q000235:genetics* / Q000502:physiology; D002339:Carotid Arteries / Q000473:pathology; D015331:Cohort Studies; D003920:Diabetes Mellitus / Q000208:ethnology / Q000235:genetics; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006630:Hispanic or Latino; D006801:Humans; D006973:Hypertension / Q000235:genetics; D007328:Insulin / Q000378:metabolism; D008297:Male; D017539:Tunica Intima / Q000473:pathology*; D017540:Tunica Media / Q000473:pathology*; D014463:Ultrasonography / Q000379:methods
chemical_list: D007328:Insulin; D002154:Calpain; C415197:calpain 10