Detection of LP2086 on the cell surface of Neisseria meningitidis and its accessibility in the presence of serogroup B capsular polysaccharide.
The outer membrane protein LP2086, a human factor H binding protein, is undergoing clinical trials as a vaccine against invasive serogroup B meningococcal (MnB) disease. As LP2086 is a surface protein, expression of capsular polysaccharide could potentially limit accessibility of anti-LP2086 antibodies to LP2086 expressed on the surface of bacteria. To determine whether variability in expression levels of the serogroup B capsule (Cap B) might interfere with accessibility of anti-LP2086 antibody binding to LP2086, we evaluated the ability of anti-Cap B and anti-LP2086 antibodies to bind to the surface of 1263 invasive clinical MnB strains by flow cytometry. One of the anti-LP2086 monoclonal antibodies used recognizes virtually all LP2086 sequence variants. Our results show no correlation between the amount of Cap B expressed and the binding of anti-LP2086 antibodies. Furthermore, the susceptibility of MnB bacteria to lysis by anti-LP2086 immune sera was independent of the level of Cap B expressed. The data presented in this paper demonstrates that Cap B does not interfere with the binding of antibodies to LP2086 expressed on the outer membrane of MnB clinical isolates.
McNeil
Lisa K
LK
Wyeth Research, 401 N. Middletown Rd., Pearl River, NY 10965, United States.
Murphy
Ellen
E
Zhao
Xiao-Juan
XJ
Guttmann
Stephen
S
Harris
Shannon L
SL
Scott
Adrienne A
AA
Tan
Cuiwen
C
Mack
Michelle
M
DaSilva
Ida
I
Alexander
Kristin
K
Mason
Kathryn
K
Jiang
Han-Qing
HQ
Zhu
Duzhang
D
Mininni
Terri L
TL
Zlotnick
Gary W
GW
Hoiseth
Susan K
SK
Jones
Thomas R
TR
Pride
Michael W
MW
Jansen
Kathrin U
KU
Anderson
Annaliesa S
AS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001770:Blood Bactericidal Activity; D005260:Female; D006801:Humans; D009345:Neisseria meningitidis / Q000737:chemistry / Q000276:immunology*; D038541:Neisseria meningitidis, Serogroup B / Q000276:immunology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C484699:factor H-binding protein, Neisseria meningitidis