Ginsenoside compound K production from ginseng root extract by a thermostable beta-glycosidase from Sulfolobus solfataricus.
Ginsenoside compound K was produced from ginseng root extract using a thermostable recombinant beta-glycosidase from Sulfolobus solfataricus. The pH and temperature for maximum production were 5.5 and 90 degrees C. The half-lives of the enzyme were 66, 30, and 1.7 h at 80, 85, and 90 degrees C respectively. The ginsenoside-hydrolyzing activity (Rd>Rb(1)>Rc>Rb(2)) and compound K-producing activity (Rd>Rc>Rb(1)>Rb(2)) of the beta-glycosidase were also determined. At pH 5.5 at 85 degrees C, 1.63 mg/ml of compound K was produced within 12 h by 40 U/ml of enzyme from 1.9 mg/ml of ginsenoside Rb(1), 0.52 mg/ml of ginsenoside Rb(2), 0.92 mg/ml of ginsenoside Rc, and 0.23 mg/ml of ginsenoside Rd in a 10% (w/v) ginseng root extract via two transformation pathways, Rb(1) or Rb(2) --> Rd --> F(2) --> compound K, and Rc --> compound Mc --> compound K.
Noh
Kyeong-Hwan
KH
Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
Son
Ju-Wan
JW
Kim
Hye-Jung
HJ
Oh
Deok-Kun
DK
D004795:Enzyme Stability; D005959:Glucosidases / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry*; D012502:Sapogenins / Q000378:metabolism*; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000201:enzymology*; D013696:Temperature
C482402:20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol; D010936:Plant Extracts; D012502:Sapogenins; D005959:Glucosidases; C104851:Sulfolobus solfataricus beta-glycosidase