[Effect of jinfu kang to experimental precancerous colon lesions and urinary metabolites in rat].
OBJECTIVE
: To profile urinary metabolite variations from 1, 2-dimethylhydrazine (DMH)-induced precancerous colon rats, Jinfu Kang treated rats and healthy controls.


METHODS
We used ethyl chloroformate derivatization and gas chromatography-mass spectrometry (GC-MS) based metabonomic method to analyze rat urines.


RESULTS
The time-dependent variations of metabolite profile showed a progressive deviation of the metabolism in the model group from the initial pattern over time and a systemic recovery of the metabolism in the treatment group, which is consistent with the histological results. The in-depth analysis indicated that the disorder of tricarboxylic acid cycle (TCA), tryptophan metabolism, polyamine metabolism and gut flora structure were associated with DMH intervention.


CONCLUSIONS
Metabolic study revealed that Jinfu Kang can effectively reverse metabolic departures in DMH-induced precancerous colon rat, which is consistent with pathological results.
Qiu
Yin-ping
YP
School of Pharmnacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Su
Ming-ming
MM
Wu
Da-zheng
DZ
Zhao
Ai-hua
AH
Liu
Yu-min
YM
Jia
Wei
W
D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology*; D003111:Colonic Polyps / Q000139:chemically induced / Q000188:drug therapy* / Q000652:urine*; D004127:Dimethylhydrazines / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004127:Dimethylhydrazines; D004365:Drugs, Chinese Herbal