title: Evaluation of tris(4,7-diphenyl-1,10-phenanthrolinedisulfonate)ruthenium(II) as a chemiluminescence reagent.
abstract: Previous studies have suggested that tris(4,7-diphenyl-1,10-phenanthrolinedisulfonate)ruthenium(II) (Ru(BPS)(3)(4-)) has great potential as a chemiluminescence reagent in acidic aqueous solution. We have evaluated four different samples of this reagent (two commercially available and two synthesised in our laboratory) in comparison with tris(2,2'-bipyridine)ruthenium(II) (Ru(bipy)(3)(2+)) and tris(1,10-phenanthroline)ruthenium(II) (Ru(phen)(3)(2+)), using a range of structurally diverse analytes. In general, Ru(BPS)(3)(4-) produced more intense chemiluminescence, but the oxidised Ru(BPS)(3)(3-) species is less stable in aqueous solution than Ru(bipy)(3)(3+) and produced a greater blank signal than Ru(bipy)(3)(3+) or Ru(phen)(3)(3+), which had a detrimental effect on sensitivity. Although the complex is often depicted with the sulfonate groups of the BPS ligand in the para position on the phenyl rings, NMR characterisation revealed that the commercially available BPS material used in this study was predominantly the meta isomer.
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.
authors.forename: Geoffrey P
authors.initials: GP
authors.lastname: McDermott
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Zammit
authors.forename: Elspeth K
authors.initials: EK
authors.lastname: Bowen
authors.forename: Michaela M
authors.initials: MM
authors.lastname: Cooke
authors.forename: Jacqui L
authors.initials: JL
authors.lastname: Adcock
authors.forename: Xavier A
authors.initials: XA
authors.lastname: Conlan
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Pfeffer
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
authors.forename: Gail A
authors.initials: GA
authors.lastname: Dyson
authors.forename: Paul S
authors.initials: PS
authors.lastname: Francis
mesh_terms: D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D008024:Ligands; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D010618:Phenanthrolines / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012996:Solutions; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D008024:Ligands; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012996:Solutions; C518776:ruthenium II tris (bathophenanthroline disulfonate); D014867:Water