CD40/CD154 system and pro-inflammatory cytokines in young healthy male smokers without additional risk factors for atherosclerosis.
OBJECTIVE
Atherosclerosis, as an inflammatory disease, is characterized by pathologically altered levels of cytokines. We investigated whether smoking affects the CD40/CD154 system and pro-inflammatory cytokines in young males without other risk factors for atherosclerosis.


METHODS
Young male smokers (n=13) and 14 non-smoking controls were investigated.


METHODS
The differences in CD40/CD154 system and serum cytokines between the groups were measured using flow cytometry and ELISA.


RESULTS
In smokers, there was a strong trend (P<0.06) for increased CD40 expression on platelets as compared with non-smokers. However, there were no significant differences in CD40 expression on monocytes or in CD154 expression on platelets and T-cells between smokers and non-smokers. There was a strong trend for increased platelet-monocyte aggregates in smokers (P<0.06). Also, smokers had slightly but not significantly elevated hsCRP and IL-6 levels, and slightly decreased TNF-alpha and MCP-1. Interestingly, IL-18, a cytokine which has the ability to promote both Th1 and Th2 responses, was significantly decreased in smokers group (P=0.03 vs controls).


CONCLUSIONS
In young healthy males, smoking is not associated with dramatic changes in CD40/CD154 system. However, cigarette smoke alters the secreted cytokine profile, leading to significant decrease in systemic IL-18 levels.
Garlichs
C D
CD
University of Erlangen-Nuremberg, Medical Clinic 2, Erlangen, Germany. Christoph.Garlichs@uk-erlangen.de
Cicha
I
I
Raaz
D
D
Meyer
L
L
Stumpf
C
C
Klinghammer
L
L
Yilmaz
A
A
Daniel
W G
WG
D000328:Adult; D050197:Atherosclerosis / Q000097:blood / Q000276:immunology*; D019013:CD40 Antigens / Q000276:immunology*; D023201:CD40 Ligand / Q000276:immunology*; D016207:Cytokines / Q000097:blood*; D006801:Humans; D020382:Interleukin-18 / Q000097:blood; D008297:Male; D012906:Smoke / Q000009:adverse effects*
D019013:CD40 Antigens; D016207:Cytokines; D020382:Interleukin-18; D012906:Smoke; D023201:CD40 Ligand