Genetic interaction between ribosome biogenesis and inositol polyphosphate metabolism in Saccharomyces cerevisiae.
Rrs1 has an essential role in 60S ribosomal subunit assembly in Saccharomyces cerevisiae. We isolated a temperature-sensitive kcs1 mutant that suppresses the cold sensitivity of rrs1-1. The kcs1 allele, resulting in truncation of inositol 6 phosphate kinase domain, and kcs1 disruption suppress a defect of rrs1-1 in 60S ribosomal subunit assembly. These results suggest that inositol polyphosphate metabolism affects ribosome biogenesis in yeast.
Horigome
Chihiro
C
Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Ikeda
Ryo
R
Okada
Takafumi
T
Takenami
Kazuhiko
K
Mizuta
Keiko
K
D000483:Alleles; D003080:Cold Temperature; D005800:Genes, Fungal; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D009687:Nuclear Proteins; C485770:RRS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010833:Phytic Acid; D017856:Phosphotransferases (Phosphate Group Acceptor); C098863:KCS1 protein, S cerevisiae