title: [Detection of the Philadelphia chromosome in adult B-lineage acute lymphoblastic leukemia using dual-color dual-fusion interphase fluorescence in situ hybridization].
abstract: OBJECTIVE
  To investigate the incidence of Philadelphia chromosome (Ph) in adult B-lineage acute lymphoblastic leukemia (B-ALL).
  
  
  METHODS
  One hundred and twelve adult patients with previously untreated B-ALL were prospectively investigated by interphase dual-color dual-fusion fluorescence in situ hybridization (DD-FISH) with two-color break apart probe BCR-ABL and the results were compared with that of conventional cytogenetics (CC).
  
  
  RESULTS
  The incidence of Ph chromosome was 17.98% (16/89) and 31.25% (35/112) by CC and DD-FISH, respectively. The mean positive rate of Ph+cells by FISH was 66.23% (ranging 18.5%-99%). Of the 35 Ph+ALL patients by FISH, 25 were successfully karyotyped by CC which included 5 normal karyotypes, 20 abnormal karyotypes including 16 Ph chromosome and 13 complex abnormalities.
  
  
  CONCLUSIONS
  The incidence of Ph chromosome was 31.25% in adult with B-ALL. DD-FISH with BCR-ABL probe provides a powerful technique for the diagnosis of Ph+B-ALL. It is an important supplement to the CC analysis. DD-FISH technique should be used as a routine method for the diagnosis for adult acute B-ALL.
authors.affiliation: Department of Hematology, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, 210029 PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Si-xuan
authors.initials: SX
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Qiu
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Qiao
authors.forename: Ming
authors.initials: M
authors.lastname: Hong
authors.forename: Jian-fu
authors.initials: JF
authors.lastname: Zhang
authors.forename: Kou-rong
authors.initials: KR
authors.lastname: Miao
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology*; D002869:Chromosome Aberrations; D003116:Color; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology*