title: Identification and characterization of a chitinase-produced bacillus showing significant antifungal activity.
abstract: Strain MY75 is a gram-positive, aerobic, endospore-forming bacterium that can secrete high levels of extracellular chitinase (4.645 U/ml) when chitin powder exists as an inducer. This strain was identified as Bacillus licheniformis using the Biolog MicroLog microbial identification system and sequence analysis of 16S rDNA, gyrA and rpoB genes. Strain MY75 has the ability to inhibit the growth of Gibberella saubinetii and Aspergillus niger, two major pathogenic fungi in agriculture, and to restrain their spore germination completely. The chitinase was proved to play an important role in the strain's antifungal activity.
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China. liangxiao@mail.nankai.edu.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.forename: Chi-Chu
authors.initials: CC
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects; D001407:Bacillus / Q000302:isolation & purification* / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D002688:Chitinases / Q000096:biosynthesis* / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005874:Gibberella / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000935:Antifungal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D002688:Chitinases; D027081:DNA Gyrase