title: Selectivity of hyaluronic acid binding for spermatozoa with normal Tygerberg strict morphology.
abstract: During spermiogenesis, a plasma membrane remodelling step facilitates formation of sperm zona pellucida and hyaluronic acid (HA) binding sites. Enrichment of Tygerberg normal spermatozoa in HA-bound versus semen sperm fractions was postulated. Semen was placed on the uncoated A side and HA-coated B side of a semen chamber. After 15 min, the HA binding score (proportion of HA-bound motile spermatozoa) was assessed on the B side, and unbound spermatozoa were removed by gentle rinsing. Following Diff-Quick staining, sperm morphology of A and B sides was evaluated by three blinded investigators at Yale and Tygerberg. The proportion of Tygerberg normal spermatozoa was higher in HA-bound versus semen spermatozoa (n = 63 subjects) with a 3.04-fold improvement (95% confidence limits: 1.9-4.7) in 37 teratozoospermic men, comparable with a 4.2-fold enrichment in zona pellucida-bound spermatozoa reported earlier by the Tygerberg group. The morphology scores of three investigators were different but related, indicating that the variations reflect individual-to-individual differences in the perception of shape normality. The selection power of HA and zona pellucida for normal spermatozoa are similar. The sperm biomarkers of creatine phosphokinase (reflecting retained cytoplasm in arrested maturity spermatozoa) and chaperone protein HspA2 (heat shock protein) were proportional with sperm HA binding. As HA binding reflects sperm maturity and function, the combination of Tygerberg morphology and HA binding is likely to improve male infertility management.
authors.affiliation: Sperm Physiology Laboratory, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Prinosilova
authors.forename: Thinus
authors.initials: T
authors.lastname: Kruger
authors.forename: Leyla
authors.initials: L
authors.lastname: Sati
authors.forename: Sinan
authors.initials: S
authors.lastname: Ozkavukcu
authors.forename: Lynne
authors.initials: L
authors.lastname: Vigue
authors.forename: Ertug
authors.initials: E
authors.lastname: Kovanci
authors.forename: Gabor
authors.initials: G
authors.lastname: Huszar
mesh_terms: D015415:Biomarkers / Q000032:analysis; D003584:Cytological Techniques; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008297:Male; D011786:Quality Control; D012107:Research Design; D013079:Sperm Maturation / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D015415:Biomarkers; D006820:Hyaluronic Acid