Cutting edge: Phenotypic characterization and differentiation of human CD8+ T cells producing IL-17.
Although IL-17-producing CD8(+) T cells (Tc17 cells) have recently been identified, the detailed information about these cells still remains unclear. In this article we describe a study investigating the phenotype and differentiation of human Tc17 cells. Human Tc17 cells were detected in a minor population of CD8(+) T cells and were predominantly found in CD27(-/+)CD28(+)CD45RA(-) memory subsets. They also expressed CCR6 and a high level of CCR5. Though most Tc17 cells produced IFN-gamma, a small part of the Tc17 cell population was IFN-gamma negative in some individuals. Analysis of the in vitro induction of Tc17 cells showed that these cells were induced from the CD27(+)CD28(+)CD45RA(+) naive subset and that they expressed the CD27(-)CD28(+)CD45RA(-) and CCR6(+) phenotype. These results together indicate that Tc17 cells have a unique phenotype and suggest that they differentiate from the same precursors that differentiate into IFN-gamma-producing CD8(+) T cells.
Kondo
Takaaki
T
Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
Takata
Hiroshi
H
Matsuki
Fumichika
F
Takiguchi
Masafumi
M
D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D010641:Phenotype; D054399:Receptors, CCR6 / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology / Q000378:metabolism
C106311:CCR6 protein, human; D018106:CD28 Antigens; D020381:Interleukin-17; D054399:Receptors, CCR6; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D017493:Leukocyte Common Antigens