title: Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique.
abstract: We describe the fabrication of three-dimensional tissue constructs using a magnetic force-based tissue engineering technique, in which cellular organization is controlled by magnetic force. Target cells were labeled with magnetite cationic liposomes (MCLs) so that the MCL-labeled cells could be manipulated by applying a magnetic field. Line patterning of human umbilical vein endothelial cells (HUVECs) labeled with MCLs was successfully created on monolayer cells or skin tissues using a magnetic concentrator device. Multilayered cell sheets were also inducible on a culture surface by accumulating MCL-labeled cells under a uniform magnetic force. Based on these results, we attempted to construct a complex multilayered myoblast C2C12 cell sheet. Here, patterned HUVECs were embedded by alternating the processes of magnetic accumulation of C2C12 cells for cell layer formation and magnetic patterning of HUVECs on the cell layers. This technique may be applicable for the fabrication of complex tissue architectures required in tissue engineering.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Akiyama
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000166:cytology*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008280:Magnetics; D051379:Mice; D032446:Myoblasts / Q000166:cytology*; D041681:NIH 3T3 Cells; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: D008081:Liposomes; D052203:Ferrosoferric Oxide