title: Azurin-poly(N-isopropylacrylamide) conjugates by site-directed mutagenesis and their thermosensitive behavior in electron-transfer processes.
abstract: Searching for "intelligence": Azurin-PNIPAM conjugates were prepared by site-directed mutagenesis followed by protein reconstitution by using imidazole-conjugated poly(N-isopropylacrylamides). The polymer-bound imidazole acts as a ligand in the active site of the blue copper protein azurin. The bioconjugates showed thermosensitive behavior in electron-transfer processes with reduced cytochrome c.
authors.affiliation: Organisch-Chemisches Institut, Westfälische Wilhelms-Universität, Corrensstrasse 40, 48149 Münster, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Rosenberger
authors.forename: Armido
authors.initials: A
authors.lastname: Studer
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takatani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakajima
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis / Q000737:chemistry*; D000180:Acrylic Resins; D001400:Azurin / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D016297:Mutagenesis, Site-Directed; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D015202:Protein Engineering; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D001400:Azurin; C052970:poly-N-isopropylacrylamide; D003300:Copper