Van Gogh and Frizzled act redundantly in the Drosophila sensory organ precursor cell to orient its asymmetric division.
Drosophila sensory organ precursor cells (SOPs) divide asymmetrically along the anterior-posterior (a-p) body axis to generate two different daughter cells. Planar Cell Polarity (PCP) regulates the a-p orientation of the SOP division. The localization of the PCP proteins Van Gogh (Vang) and Frizzled (Fz) define anterior and posterior apical membrane domains prior to SOP division. Here, we investigate the relative contributions of Vang, Fz and Dishevelled (Dsh), a membrane-associated protein acting downstream of Fz, in orienting SOP polarity. Genetic and live imaging analyses suggest that Dsh restricts the localization of a centrosome-attracting activity to the anterior cortex and that Vang is a target of Dsh in this process. Using a clone border assay, we provide evidence that the Vang and fz genes act redundantly in SOPs to orient its polarity axis in response to extrinsic local PCP cues. Additionally, we find that the activity of Vang is dispensable for the non-autonomous polarizing activity of fz. These observations indicate that both Vang and Fz act as cues for downstream effectors orienting the planar polarity axis of dividing SOPs.
Gomes
José-Eduardo
JE
Ecole Normale Supérieure, CNRS UMR8542, Paris, France.
Corado
Maria
M
Schweisguth
François
F
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000705:Anaphase / Q000502:physiology; D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000502:physiology*; D016764:Cell Polarity; D018385:Centrosome / Q000378:metabolism; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000502:physiology; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D020730:Guanine Nucleotide Dissociation Inhibitors; D008565:Membrane Proteins; D010750:Phosphoproteins; C405164:Pins protein, Drosophila; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; C111638:Vang protein, Drosophila; C000606588:dsh protein, Drosophila; C058784:fz protein, Drosophila