Selected nondigestible carbohydrates and prebiotics support the growth of probiotic fish bacteria mono-cultures in vitro.
OBJECTIVE
To search for nondigestible but fermentable (NDF) carbohydrates and prebiotics with a potency to promote the growth of selected bacteria in vitro.


RESULTS
The growth of three reference bacteria strains Bacillus subtilis LMG 7135(T), Carnobacterium piscicola LMG 9839, Lactobacillus plantarum LMG 9211 and one candidate probiotic bacteria Lactobacillus delbrueckii subsp. lactis was investigated over a minimum period of 48 h in the presence of beta-glucan, xylo-oligosaccharide, arabinoxylo-oligosaccharide, inulin, oligofructose and glucose. Besides the capability to grow on inulin and oligofructose containing media, a distinct high growth in beta-glucan based substrates and a low growth in (arabino)xylooligosaccharide containing media were evident for most bacteria tested. With the exception of B. subtilis and L. plantarum, other bacteria grew equally well or even better on different substrates than on glucose. The fermentation of studied carbohydrates by these micro-organisms was dominated by the production of acetic acid as the main short chain fatty acid.


CONCLUSIONS
Selected bacteria are able to ferment and grow on NDF and prebiotic carbohydrates but in a substrate dependent manner.


CONCLUSIONS
This study delivers a first screening of which NDF or prebiotic carbohydrates are the most promising for aquaculture feed supplementations.
Rurangwa
E
E
Laboratory of Aquatic Ecology and Evolutionary Biology, Katholieke Universiteit Leuven, Leuven, Belgium. eugene.rurangwa@bio.kuleuven.be
Laranja
J L
JL
Van Houdt
R
R
Delaedt
Y
Y
Geraylou
Z
Z
Van de Wiele
T
T
Van Loo
J
J
Van Craeyveld
V
V
Courtin
C M
CM
Delcour
J A
JA
Ollevier
F
F
D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D001412:Bacillus subtilis / Q000254:growth & development; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D056573:Carnobacterium / Q000254:growth & development; D015169:Colony Count, Microbial; D003470:Culture Media; D005399:Fishes / Q000382:microbiology; D005947:Glucose / Q000378:metabolism; D007444:Inulin / Q000378:metabolism; D007778:Lactobacillus / Q000254:growth & development; D009844:Oligosaccharides / Q000378:metabolism; D056692:Prebiotics; D019936:Probiotics; D047071:beta-Glucans / Q000378:metabolism
D003470:Culture Media; D009844:Oligosaccharides; D056692:Prebiotics; D047071:beta-Glucans; C120489:oligofructose; D007444:Inulin; D005947:Glucose; D019342:Acetic Acid