Genetic engineering of Caulobacter crescentus for removal of cadmium from water.
Hexa-histidine (6His) peptide was inserted to a permissive site of the surface layer (S-layer) protein RsaA of Caulobacter crescentus. The recombinant strain JS4022/p723-6H, expressing RsaA-6His fusion protein was examined for its ability to sequester Cd(II) from the bacterial growth medium. When mixed with 1 ppm CdCl(2), JS4022/p723-6H removed 94.3 approximately 99.9% of the Cd(II), whereas the control strain removed only 11.4 approximately 37.0%, depending on experimental conditions. The effective contact time of the cells and Cd(II) was as short as 15 min. When higher concentrations of CdCl(2) were tested, JS4022/p723-6H consistently demonstrated enhanced binding capacity over the control strain. At 15 ppm of Cd(II), each gram of JS4022/p723-6H dry cells retrieved 16.0 mg of Cd(II), comparing to 11.6 mg g(-1) achieved by the control strain. This work provides a potential cost-effective solution toward bioremediation of heavy metals from aqueous systems.
Patel
Jigar
J
Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0208, USA.
Zhang
Qiong
Q
McKay
R Michael L
RM
Vincent
Robert
R
Xu
Zhaohui
Z
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002104:Cadmium / Q000302:isolation & purification* / Q000378:metabolism*; D016935:Caulobacter crescentus / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D049109:Cell Proliferation; D005818:Genetic Engineering; D012996:Solutions; D013997:Time Factors; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
D001426:Bacterial Proteins; D012996:Solutions; D014874:Water Pollutants, Chemical; D002104:Cadmium; D014867:Water