title: Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells.
abstract: The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 microm feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful 'hands-on' tool for the generation of micro-patterned chemical or biological assays using cells and proteins.
authors.affiliation: Department of Bio and Brain Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Korea.
authors.forename: Hyundoo
authors.initials: H
authors.lastname: Hwang
authors.forename: Gyumin
authors.initials: G
authors.lastname: Kang
authors.forename: Ju Hun
authors.initials: JH
authors.lastname: Yeon
authors.forename: Yoonkey
authors.initials: Y
authors.lastname: Nam
authors.forename: Je-Kyun
authors.initials: JK
authors.lastname: Park
mesh_terms: D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon