title: The Trypanosoma cruzi nucleic acid binding protein Tc38 presents changes in the intramitochondrial distribution during the cell cycle.
abstract: BACKGROUND
  Tc38 of Trypanosoma cruzi has been isolated as a single stranded DNA binding protein with high specificity for the poly [dT-dG] sequence. It is present only in Kinetoplastidae protozoa and its sequence lacks homology to known functional domains. Tc38 orthologues present in Trypanosoma brucei and Leishmania were proposed to participate in quite different cellular processes. To further understand the function of this protein in Trypanosoma cruzi, we examined its in vitro binding to biologically relevant [dT-dG] enriched sequences, its expression and subcellular localization during the cell cycle and through the parasite life stages.
  
  
  RESULTS
  By using specific antibodies, we found that Tc38 protein from epimastigote extracts participates in complexes with the poly [dT-dG] probe as well as with the universal minicircle sequence (UMS), a related repeated sequence found in maxicircle DNA, and the telomeric repeat. However, we found that Tc38 predominantly localizes into the mitochondrion. Though Tc38 is constitutively expressed through non-replicating and replicating life stages of T. cruzi, its subcellular localization in the unique parasite mitochondrion changes according to the cell cycle stage. In epimastigotes, Tc38 is found only in association with kDNA in G1 phase. From the S to G2 phase the protein localizes in two defined and connected spots flanking the kDNA. These spots disappear in late G2 turning into a diffuse dotted signal which extends beyond the kinetoplast. This later pattern is more evident in mitosis and cytokinesis. Finally, late in cytokinesis Tc38 reacquires its association with the kinetoplast. In non-replicating parasite stages such as trypomastigotes, the protein is found only surrounding the entire kinetoplast structure.
  
  
  CONCLUSIONS
  The dynamics of Tc38 subcellular localization observed during the cell cycle and life stages support a major role for Tc38 related to kDNA replication and maintenance.
authors.affiliation: Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Montevideo, Uruguay. mduhagon@fcien.edu.uy
authors.forename: María A
authors.initials: MA
authors.lastname: Duhagon
authors.forename: Lucía
authors.initials: L
authors.lastname: Pastro
authors.forename: José R
authors.initials: JR
authors.lastname: Sotelo-Silveira
authors.forename: Leticia
authors.initials: L
authors.lastname: Pérez-Díaz
authors.forename: Dante
authors.initials: D
authors.lastname: Maugeri
authors.forename: Sheila C
authors.initials: SC
authors.lastname: Nardelli
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.forename: Noreen
authors.initials: N
authors.lastname: Williams
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
authors.forename: Beatriz
authors.initials: B
authors.lastname: Garat
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018105:DNA, Kinetoplast / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry*; D011485:Protein Binding; D014349:Trypanosoma cruzi / Q000502:physiology*
chemical_list: D018105:DNA, Kinetoplast; D004268:DNA-Binding Proteins; C477882:Tc38 protein, Trypanosoma cruzi