title: Lack of genetic variation in tree ring delta13C suggests a uniform, stomatally-driven response to drought stress across Pinus radiata genotypes.
abstract: We assessed the variation in delta(13)C signatures of Pinus radiata D. Don stemwood taken from three genetic trials in southern Australia. We sought to determine the potential of using delta(13)C signatures as selection criteria for drought tolerance. Increment cores were taken from P. radiata and were used to determine the basal area increment and the delta(13)C signature of extracted cellulose. Both growth increment and cellulose delta(13)C were affected by water availability. Growth increment and delta(13)C were negatively correlated suggesting that growth was water-limited. While there was significant genetic variation in growth, there was no significant genetic variation in cellulose delta(13)C of tree rings. This suggests that different genotypes of P. radiata display significant differences in growth and yet respond similarly to drought stress. The delta(13)C response to drought stress was more due to changes in stomatal conductance than to the variation in photosynthetic capacity, and this may explain the lack of genetic variation in delta(13)C. The lack of genetic variation in cellulose delta(13)C of tree rings precludes its use as a selection criterion for drought tolerance among P. radiata genotypes.
authors.affiliation: Department of Forest and Ecosystem Science, School of Land and Environment, University of Melbourne, Parkville VIC 3010, Australia. rowelldm@unimelb.edu.au
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Rowell
authors.forename: Peter K
authors.initials: PK
authors.lastname: Ades
authors.forename: Michael
authors.initials: M
authors.lastname: Tausz
authors.forename: Stefan K
authors.initials: SK
authors.lastname: Arndt
authors.forename: Mark A
authors.initials: MA
authors.lastname: Adams
mesh_terms: D000222:Adaptation, Physiological; D002244:Carbon / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D003681:Dehydration; D055864:Droughts; D017343:Genes, Plant; D014644:Genetic Variation; D005838:Genotype; D010788:Photosynthesis / Q000502:physiology*; D028223:Pinus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D054046:Plant Stomata; D013312:Stress, Physiological; D014197:Trees / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D014934:Wood / Q000235:genetics / Q000378:metabolism; D052584:Xylem / Q000235:genetics / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D002244:Carbon