(-)-Epigallocatechin-3-gallate enhances uncoupling protein 2 gene expression in 3T3-L1 adipocytes.
In this study, we investigated the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the mRNA level and promoter activity of uncoupling protein 2 (UCP2), a mitochondrial membrane transporter that regulates energy expenditure and thermogenesis in 3T3-L1 adipocytes. EGCG up-regulated the UCP2 mRNA level in a dose-dependent manner. UCP2 promoter activity was significantly stimulated by EGCG treatment, to an extent similar to that seen in mRNA expression. These results suggest that expression of UCP2 gene is directly regulated by green tea EGCG, which is mediated through the transcriptional activation of its proximal promoter.
Lee
Mak-Soon
MS
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea.
Kim
Yangha
Y
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000071246:Uncoupling Protein 2
D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D002392:Catechin; C045651:epigallocatechin gallate