Id-1 expression in androgen-dependent prostate cancer is negatively regulated by androgen through androgen receptor.
This study discovered that Id-1 expression in androgen-dependent prostate cancer decreased immediately after androgen deprivation but increased after longer androgen deprivation both in vivo and in vitro. Id-1 expression in androgen-independent LNCaP cells was about 6 fold as that in their parental cells. As was the case with LNCaP cells, when androgen receptor (AR) was introduced into AR-negative PC-3 cells, dihydrotestosterone inhibited while flutamide increased Id-1 expression. Thus, Id-1 expression in androgen-dependent prostate cancer was negatively regulated by androgen in a receptor-dependent way. The re-increased Id-1 might partially contribute to the emergence of androgen-independent prostate cancer after longer androgen deprivation therapy.
Xu
Bin
B
Department of Urology, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China.
Sun
Yinghao
Y
Department of Urology, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China. Electronic address: sunyh@medmail.com.cn.
Tang
Gusheng
G
Department of Laboratory Diagnosis, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China.
Xu
Chuanliang
C
Department of Urology, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China.
Wang
Linhui
L
Department of Urology, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China.
Zhang
Yuxi
Y
Department of Urology, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China.
Ji
Jiatao
J
Department of Urology, Changhai Hospital, 174 Changhai Road, Shanghai 200433, China.
D000728:Androgens / Q000502:physiology*; D045744:Cell Line, Tumor; D013196:Dihydrotestosterone / Q000494:pharmacology; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000737:chemistry* / Q000628:therapy; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000628:therapy; D012333:RNA, Messenger / Q000032:analysis; D011944:Receptors, Androgen / Q000502:physiology*
C410798:AR protein, human; D000728:Androgens; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D012333:RNA, Messenger; D011944:Receptors, Androgen; D013196:Dihydrotestosterone