Selection and validation of candidate housekeeping genes for studies of human keratinocytes--review and recommendations.
Gene expression analysis using real-time PCR has become an integral part of biomedical research. Appropriate data normalization based on stably expressed housekeeping genes is crucial for reliable results. Thus, candidate housekeeping genes require careful evaluation with regard to the individual experimental system before being selected for studies of human keratinocytes. Future research may be based on published data, as provided by Minner and Poumay in this issue.
Bär
Michael
M
Department of Dermatology, Carl Gustav Carus Medical School Dresden, Germany. Michael.Baer@uniklinikum-dresden.de
Bär
Dorit
D
Lehmann
Bodo
B
D002454:Cell Differentiation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005823:Genetics; D006801:Humans; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000378:metabolism*; D008954:Models, Biological; D010100:Oxygen / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D012333:RNA, Messenger; D010100:Oxygen