title: Inflammatory and oxidative stress markers in experimental crystalopathy: their modification by photostimulation.
abstract: Crystalopathies are inflammatory pathologies caused by cellular reactions to the deposition of crystals in the joints. The anti-inflammatory effect of the helium-neon (He-Ne) laser and that of the nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac, meloxicam, celecoxib, and rofecoxib was studied in acute and chronic arthritis produced by hydroxyapatite and calcium pyrophosphate in rats. The presence of the markers fibrinogen, L-citrulline, nitric oxide, and nitrotyrosine was determined. Crystals were injected into the posterior limb joints of the rats. A dose of 8 J/cm(2) of energy from an He-Ne laser was applied for 3 d in some groups and for 5 d in other groups. The levels of some of the biomarkers were determined by spectrophotometry, and that of nitrotyrosine was determined by ELISA. For statistical analysis, Fisher's exact test was used, and p +/- 0.05 was considered significant. In arthritic rats, the fibrinogen, L-citrulline, nitric oxide, and nitrotyrosine levels increased in comparison to controls and to the laser-treated arthritic groups (p +/- 0.001), (p +/- 0.001), (p +/- 0.02), and (p +/- 0.01), respectively. When comparing fibrinogen from arthritic rats with disease induced by hydroxyapatite with undiseased and arthritic rats treated with NSAIDs, the He-Ne laser decreased levels to values similar to those seen in controls (p +/- 0.01). Inflammatory and oxidative stress markers in experimental crystalopathy are positively modified by photobiostimulation.
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Claudia Reinoso
authors.initials: CR
authors.lastname: Rubio
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Simes
authors.forename: Monica
authors.initials: M
authors.lastname: Moya
authors.forename: Fernando
authors.initials: F
authors.lastname: Soriano
authors.forename: Jose Atilio
authors.initials: JA
authors.lastname: Palma
authors.forename: Vilma
authors.initials: V
authors.lastname: Campana
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001168:Arthritis / Q000188:drug therapy / Q000378:metabolism / Q000532:radiotherapy*; D015415:Biomarkers / Q000097:blood; D002131:Calcium Pyrophosphate / Q000008:administration & dosage; D002956:Citrulline / Q000097:blood; D003460:Crystallization; D017886:Durapatite / Q000008:administration & dosage; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006614:Hindlimb; D007249:Inflammation; D007270:Injections, Intra-Articular; D054020:Lasers, Gas / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D051381:Rats
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D002956:Citrulline; D009569:Nitric Oxide; D005340:Fibrinogen; D017886:Durapatite; D002131:Calcium Pyrophosphate