In vitro osteoblast differentiation is negatively regulated by Hoxc8.
Hoxc8 has multiple roles in normal skeletal development. In this paper, a MC3T3-E1 subclone 4 osteogenic cell differentiation model was used to examine expression of Hoxc8 at multiple stages of osteogenesis. We found that Hoxc8 expression levels do not change in the early stage but increase in the middle stage and decrease in the late stage of osteogenesis. A knockdown of Hoxc8 by small-interfering RNA transfection in C2C12 cells indicated that Hoxc8 is a negative regulator of osteogenesis. Similarly, expression of Hoxc8 in C2C12 cells decreases alkaline phosphatase levels induced by bone morphogenetic protein-2 (BMP-2). The results of this study showed that Hoxc8 is involved in BMP-2-induced osteogenesis, and osteoblast differentiation in vitro is negatively regulated by Hoxc8, suggesting that Hoxc8 regulation is essential for osteoblast differentiation.
Zheng
Yan-Jun
YJ
Department of Anatomy, Embryology Lab, Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University, 134 Seodaemun-gu, Shinchon-dong, 120-752 Seoul, South Korea.
Chung
Hyun Joo
HJ
Min
Hyehyun
H
Kang
Myengmo
M
Kim
Seong Hwan
SH
Gadi
Jogeswar
J
Kim
Myoung Hee
MH
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
D019485:Bone Morphogenetic Proteins; D018398:Homeodomain Proteins; C489816:Hoxc8 protein, mouse; D034741:RNA, Small Interfering