The expression of PADI4 in synovium of rheumatoid arthritis.
PADI4 that catalyzes the conversion of peptidylarginine to citrulline is associated with rheumatoid arthritis in some populations. The current study investigated the expressions of PADI4 in synovial fluid of RA (n = 73), osteoarthritis (OA, n = 96) and ankylosing spondylitis (AS, n = 32) using ELISA and western blotting following immuno-precipitation (n = 6 for each diseases). The study also compared the mRNA level of PADI4 in the synovial membrane of RA with the levels in the samples of OA and AS (n = 6 for each diseases) using real time PCR. ELISA detected a higher level of PADI4 in SF of RA than in samples of OA and AS (P = 0.0001). The level of PADI4 was significantly correlated with the level of rheumatic factor (P = 0.015), but not with anti cyclic citrullinated peptide antibody (anti-CCP) in the RA fluids. Western blotting confirmed the expression of PADI4 in SF of RA. Quantitative PCR measured higher transcription of PADI4 in the synovial membrane of RA than in the samples of OA and AS. The results confirmed increased expression of PADI4 in synovium of RA.
Chang
Xiaotian
X
Key Laboratory for Bio-drugs of Ministry of Health, Research Center for Medicinal Biotechnology, Shandong Academy of Medical Sciences, 250062 Jinan, Shandong, People's Republic of China. changxt@sdams.cn
Zhao
Yan
Y
Sun
Shui
S
Zhang
Yunzhong
Y
Zhu
Youming
Y
D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D002116:Calcitonin / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000276:immunology; D011498:Protein Precursors / Q000097:blood; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; D012333:RNA, Messenger / Q000378:metabolism; D012217:Rheumatoid Factor / Q000378:metabolism; D013167:Spondylitis, Ankylosing / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D010456:Peptides, Cyclic; D011498:Protein Precursors; D012333:RNA, Messenger; C487763:cyclic citrullinated peptide; D002116:Calcitonin; D002097:C-Reactive Protein; D012217:Rheumatoid Factor; D006867:Hydrolases; C000629235:PADI4 protein, human; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases