Transcriptome analysis during somatic embryogenesis of the tropical monocot Elaeis guineensis: evidence for conserved gene functions in early development.
With the aim of understanding the molecular mechanisms underlying somatic embryogenesis (SE) in oil palm, we examined transcriptome changes that occur when embryogenic suspension cells are initiated to develop somatic embryos. Two reciprocal suppression subtractive hybridization (SSH) libraries were constructed from oil palm embryogenic cell suspensions: one in which embryo development was blocked by the presence of the synthetic auxin analogue 2,4-dichlorophenoxyacetic acid (2,4-D: ) in the medium (proliferation library); and another in which cells were stimulated to form embryos by the removal of 2,4-D: from the medium (initiation library). A total of 1867 Expressed Sequence Tags (ESTs) consisting of 1567 potential unigenes were assembled from the two libraries. Functional annotation indicated that 928 of the ESTs correspond to proteins that have either no similarity to sequences in public databases or are of unknown function. Gene Ontology (GO) terms assigned to the two EST populations give clues to the underlying molecular functions, biological processes and cellular components involved in the initiation of embryo development. Macroarrays were used for transcript profiling the ESTs during SE. Hierarchical cluster analysis of differential transcript accumulation revealed 4 distinct profiles containing a total of 192 statistically significant developmentally regulated transcripts. Similarities and differences between the global results obtained with in vitro systems from dicots, monocots and gymnosperms will be discussed.
Lin
Hsiang-Chun
HC
IRD, UMR DIAPC, IRD/CIRAD Palm Development Group, 911 Avenue Agropolis, BP 64501, 34394, Montpellier Cedex 5, France.
Morcillo
Fabienne
F
Dussert
Stéphane
S
Tranchant-Dubreuil
Christine
C
Tregear
James W
JW
Tranbarger
Timothy John
TJ
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D028023:Arecaceae / Q000196:embryology* / Q000235:genetics*; D002478:Cells, Cultured; D016000:Cluster Analysis; D003470:Culture Media; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018745:Genome, Plant; D017422:Sequence Analysis, DNA
D003470:Culture Media; D018744:DNA, Plant; D015084:2,4-Dichlorophenoxyacetic Acid