Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Chen
Alice E
AE
Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
Egli
Dieter
D
Niakan
Kathy
K
Deng
Jie
J
Akutsu
Hidenori
H
Yamaki
Mariko
M
Cowan
Chad
C
Fitz-Gerald
Claire
C
Zhang
Kun
K
Melton
Douglas A
DA
Eggan
Kevin
K
D000818:Animals; D053624:Blastocyst Inner Cell Mass / Q000166:cytology / Q000502:physiology*; D000071616:CDX2 Transcription Factor; D018929:Cell Culture Techniques; D002460:Cell Line; D002478:Cells, Cultured; D016000:Cluster Analysis; D053595:Embryonic Stem Cells / Q000502:physiology*; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism
D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human