title: Evaluation of urothelial stretch-induced cyclooxygenase-2 expression in a mouse ureteral obstruction model.
abstract: Prostanoids play a major role in the nociceptive response to ureteral obstruction. Cyclooxygenases (COXs) 1 and 2 catalyze the rate-limiting step in prostanoid synthesis; COX-2 is the more inducible isoform. Previous studies in human and animal models have shown that COX-2 is highly induced during ureteral obstruction. Our objective was to characterize acute COX-2 induction in a reproducible mouse model. Unilateral ureteral ligation was performed, and obstruction was maintained for 2, 4, 6, 8, or 12 hours. We evaluated COX-2 protein expression using Western immunoblotting, and found that ureteral obstruction induced COX-2 expression ninefold within 6 hours. This is the first report to characterize in vivo temporal stretch-induced COX-2 expression in a mouse model. This model will be critical for elucidation of COX-2 signaling pathways and may eventually help to identify novel therapeutic targets for treating ureteral obstruction.
authors.affiliation: Department of Urology, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Samuel P
authors.initials: SP
authors.lastname: Sterrett
authors.forename: Eric R
authors.initials: ER
authors.lastname: Wilkinson
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Jerde
authors.forename: William S
authors.initials: WS
authors.lastname: Mellon
authors.forename: Stephen Y
authors.initials: SY
authors.lastname: Nakada
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D015151:Immunoblotting; D051379:Mice; D013314:Stress, Mechanical; D013537:Sutures; D013997:Time Factors; D014517:Ureteral Obstruction / Q000201:enzymology*; D019459:Urothelium / Q000201:enzymology*
chemical_list: C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2