[Studies on chemical constituents from herbs of Botrychium lanuginosum].
OBJECTIVE
To study the chemical constituents of Botrychium lanuginosum.


METHODS
Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated by chemical evidence and spectroscopic methods.


RESULTS
Ten compounds were isolated from the 95% ethanol extract of the herb of B. lanuginosum and their structures were elucidated as 30-nor-21beta-hopan-22-one (1), beta-sitosterol (2), luteolin (3), thunberginol A (4), apigenin (5), (6'-O-palmitoyl)-sitosterol-3-O-beta-D-glucoside (6), daucosterol (7), 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2R-hydroxy hexadecanoyl) amino]-4, 8-octadecadiene-1, 3-diol (8), luteolin-7-O-glucoside (9), sucrose (10).


CONCLUSIONS
Compounds 1-10 were isolated from this genus for the first time.
Wang
Dong
D
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China.
Liu
Xiao-qiu
XQ
Yao
Chun-suo
CS
Fang
Wei-shuo
WS
D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification; D002845:Chromatography; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D029624:Ferns / Q000737:chemistry*; D049934:Isocoumarins / Q000737:chemistry / Q000302:isolation & purification; D047311:Luteolin / Q000737:chemistry / Q000302:isolation & purification; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification; D013395:Sucrose / Q000737:chemistry / Q000302:isolation & purification
D004365:Drugs, Chinese Herbal; D049934:Isocoumarins; D012855:Sitosterols; C079055:thunberginol A; D013395:Sucrose; C025473:gamma-sitosterol; D047310:Apigenin; D047311:Luteolin; C011015:lyoniside