title: The breakdown of preformed peritoneal advanced glycation end products by intraperitoneal alagebrium.
abstract: It has been demonstrated that inhibitors of advanced glycation end products (AGE), such as aminoguanidine, can suppress peritoneal AGE in rats on peritoneal dialysis (PD). However, it is unknown whether late administration of a putative cross-link breaker, alagebrium, could reverse peritoneal AGE. We therefore compared alagebrium with aminoguanidine in their ability to reverse peritoneal AGE in rats on PD. Male Sprague-Dawley rats were randomly divided into 3 groups: group I dialyzed with 4.25% glucose solution for all exchanges; group II dialyzed with 4.25% glucose solution containing aminoguanidine, and group III dialyzed with 4.25% glucose solution containing alagebrium for last 8 weeks of 12-week dialysis period. Dialysis exchanges were performed 2 times a day for 12 weeks. Immunohistochemistry was performed using a monoclonal anti-AGE antibody. One-hour PET was performed for comparison of transport characteristics. The immunolabelling of AGE in peritoneal membrane was markedly decreased in the alagebrium group. Consistent with this, the alagebrium group exhibited significantly higher D/Do glucose and lower D/P urea, suggesting low peritoneal membrane transport. But there were no significant differences between the control and the aminoguanidine group. These results suggest that the alagebrium may be the optimal therapeutic approach, compared with treatment with inhibitors of AGE formation, in rats on PD.
authors.affiliation: Department of Internal Medicine, Dongguk University Medical Center, Gyeongju, Korea.
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Lee
authors.forename: Joon-Yeop
authors.initials: JY
authors.lastname: Lee
authors.forename: Jun-Seup
authors.initials: JS
authors.lastname: Kim
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Won
authors.forename: Hyeok-Joo
authors.initials: HJ
authors.lastname: Kang
authors.forename: Tae-Jung
authors.initials: TJ
authors.lastname: Jang
authors.forename: Woo-Taek
authors.initials: WT
authors.lastname: Tak
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001692:Biological Transport; D001835:Body Weight; D002462:Cell Membrane / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006146:Guanidines / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D010530:Peritoneal Dialysis / Q000379:methods*; D010537:Peritoneum / Q000378:metabolism / Q000473:pathology*; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017127:Glycation End Products, Advanced; D006146:Guanidines; C004479:pimagedine