Differentially expressed genes in eutopic and ectopic endometrium of women with endometriosis.
OBJECTIVE
To elucidate the potential mechanisms involved in the physiopathology of endometriosis. We analyzed the differential gene expression profiles of eutopic and ectopic tissues from women with endometriosis.


METHODS
Prospective laboratory study.


METHODS
University hospital.


METHODS
Seventeen patients in whom endometriosis was diagnosed and 11 healthy fertile women.


METHODS
Endometrial biopsy specimens from the endometrium of healthy women without endometriosis and from the eutopic and ectopic endometrium tissues of patients with endometriosis were obtained in the early proliferative phase of the menstrual cycle.


METHODS
Six paired samples of eutopic and ectopic tissue were analyzed by subtractive hybridization. To evaluate the expression of genes found by rapid subtraction hybridization methods, we measured CTGF, SPARC, MYC, MMP, and IGFBP1 genes by real-time polymerase chain reaction in all samples.


RESULTS
This study identified 291 deregulated genes in the endometriotic lesions. Significant expression differences were obtained for SPARC, MYC, and IGFBP1 in the peritoneal lesions and for MMP3 in the ovarian endometriomas. Additionally, significant differences were obtained for SPARC and IGFBP1 between the peritoneal and ovarian lesions. No significant differences were found for the studied genes between the control and the eutopic endometrium.


CONCLUSIONS
This study identified 291 genes with differential expression in endometriotic lesions. The deregulation of the SPARC, MYC, MMP3, and IGFBPI genes may be responsible for the loss of cellular homeostasis in endometriotic lesions.
Meola
Juliana
J
Department of Genetics, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. jumeola@yahoo.com.br
Rosa e Silva
Júlio César
JC
Dentillo
Daniel Blassioli
DB
da Silva
Wilson Araújo
WA
Veiga-Castelli
Luciana Caricati
LC
Bernardes
Luciano Angelo de Souza
LA
Ferriani
Rui Alberto
RA
de Paz
Cláudia Cristina Paro
CC
Giuliatti
Silvana
S
Martelli
Lúcia
L
D000293:Adolescent; D000328:Adult; D000465:Algorithms; D002828:Choristoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D016259:Genes, myc; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D010532:Peritoneal Diseases / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
C526317:CCN2 protein, human; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D015676:Osteonectin; D055513:Connective Tissue Growth Factor; D019278:Matrix Metalloproteinase 3