title: Assaying of autophagic protein degradation.
abstract: Macroautophagy is a three-step process: (1) autophagosomes form and mature, (2) the autophagosomes fuse with lysosomes, and (3) the autophagic cargo is degraded in the lysosomes. It is this lysosomal degradation of the autophagic cargo that constitutes the autophagic flux. As in the case of metabolic pathways, the steady-state concentration of the intermediary autophagic structures alone is insufficient for investigating the flux. Assaying the degradation of long-lived proteins as described in this chapter is one of the methods that can be used to measure autophagic flux.
authors.affiliation: INSERM U756, Université Paris-Sud 11, Châtenay-Malabry, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Bauvy
authors.forename: Alfred J
authors.initials: AJ
authors.lastname: Meijer
authors.forename: Patrice
authors.initials: P
authors.lastname: Codogno
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D019073:HT29 Cells; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: D011506:Proteins