title: Nanomole-scale protein solid-state NMR by breaking intrinsic 1HT1 boundaries.
abstract: We present an approach that accelerates protein solid-state NMR 5-20-fold using paramagnetic doping to condense data-collection time (to approximately 0.2 s per scan), overcoming a long-standing limitation on slow recycling owing to intrinsic (1)H T(1) longitudinal spin relaxation. Using low-power schemes under magic-angle spinning at 40 kHz, we obtained two-dimensional (13)C-(13)C and (13)C-(15)N solid-state NMR spectra for several to tens of nanomoles of beta-amyloid fibrils and ubiquitin in 1-2 d.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, Chicago, IL 60607, USA.
authors.forename: Nalinda P
authors.initials: NP
authors.lastname: Wickramasinghe
authors.forename: Sudhakar
authors.initials: S
authors.lastname: Parthasarathy
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Jones
authors.forename: Chhavi
authors.initials: C
authors.lastname: Bhardwaj
authors.forename: Fei
authors.initials: F
authors.lastname: Long
authors.forename: Mrignayani
authors.initials: M
authors.lastname: Kotecha
authors.forename: Shahila
authors.initials: S
authors.lastname: Mehboob
authors.forename: Leslie W-M
authors.initials: LW
authors.lastname: Fung
authors.forename: Jaan
authors.initials: J
authors.lastname: Past
authors.forename: Ago
authors.initials: A
authors.lastname: Samoson
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ishii
mesh_terms: D000682:Amyloid / Q000737:chemistry; D016229:Amyloid beta-Peptides / Q000737:chemistry; D002247:Carbon Isotopes / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D006801:Humans; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D013049:Spectrin / Q000737:chemistry; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010446:Peptide Fragments; D025801:Ubiquitin; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); D013049:Spectrin; D004492:Edetic Acid