title: Anti-inflammatory sesquiterpenoids from a sponge-derived Fungus Acremonium sp.
abstract: In the course of our search for bioactive metabolites from a sponge-derived fungus Acremonium sp., new sesquiterpenoids (1-4) were isolated along with known derivatives by bioactivity-guided fractionation. The unique cyclic skeleton of compounds 2 and 3 is unprecedented. The absolute configurations were determined by modified Mosher's method and CD spectroscopy, along with comparison of (1)H and (13)C NMR spectroscopic data and specific optical rotation values with those reported. The anti-inflammatory activity of the isolated compounds (1, 5, 7-13) was evaluated by measuring their inhibitory effects on the production of pro-inflammatory mediators (NO, IL-6, and TNF-alpha) in RAW 264.7 murine macrophage cells. Among the compounds tested, compounds 7 and 9 significantly inhibited the production of NO and TNF-alpha at the concentration of 100 microM, while compounds 11 and 12 showed selective inhibition of NO production at the same concentration.
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Korea.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Baoquan
authors.initials: B
authors.lastname: Bao
authors.forename: Hung The
authors.initials: HT
authors.lastname: Dang
authors.forename: Jongki
authors.initials: J
authors.lastname: Hong
authors.forename: Hye Ja
authors.initials: HJ
authors.lastname: Lee
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Yoo
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
authors.forename: Jee H
authors.initials: JH
authors.lastname: Jung
mesh_terms: D000164:Acremonium / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D011161:Porifera / Q000382:microbiology*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015850:Interleukin-6; D012717:Sesquiterpenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide