title: Enzymatic synthesis of peptides on a solid support.
abstract: We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.
authors.affiliation: Manchester Interdisciplinary Biocentre, The University of Manchester and School of Chemistry, 131 Princess Street, Manchester, UK M1 7DN.
authors.forename: Rose
authors.initials: R
authors.lastname: Haddoub
authors.forename: Martin
authors.initials: M
authors.lastname: Dauner
authors.forename: Fiona A
authors.initials: FA
authors.lastname: Stefanowicz
authors.forename: Valeria
authors.initials: V
authors.lastname: Barattini
authors.forename: Nicolas
authors.initials: N
authors.lastname: Laurent
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D000180:Acrylic Resins; D000596:Amino Acids / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D006031:Glycosylation; D008722:Methods; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols; D014867:Water
chemical_list: D000180:Acrylic Resins; D000596:Amino Acids; D004798:Enzymes; D010455:Peptides; C520358:poly(acryloyl-bis(aminopropyl)polyethylene glycol); D014867:Water; D011092:Polyethylene Glycols; D010447:Peptide Hydrolases