Preincubation with bFGF but not sodium ascorbate improves efficiency of autologous transplantation of muscle-derived cells into urethral wall.
OBJECTIVE
Myogenic cell therapy is considered a new method to treat urinary incontinence. One of the crucial limitations in cellular transplantation is poor cell survival after cell transfer. Preconditioning of cells before transfer is a new approach in improving the efficacy of graft.


METHODS
In this study, autologous cell transplantation was performed on a rat model. Muscle-derived cells were isolated, identified with immunofluorescence and immunoblotting, preincubated or not with 10 ng/mL of basic fibroblast growth factor (bFGF) or 0.1 mM of sodium ascorbate for 24 hours, and transfected with the beta-galactosidase encoding gene using lipofectamine. Cell suspension was injected into the urethral wall. The tissue was collected 2 weeks after injection.


RESULTS
The presence of transplanted cells was confirmed by X-Gal staining. Moreover, the effects of preincubation with either sodium ascorbate or bFGF 24 hours before transfer were evaluated. A semiqualitative analysis using chlorophenol-red-beta-d-galactopyranoside substrate demonstrated that preincubation with ascorbate had no effect on the number of transplanted cells but that bFGF significantly increased the amount of cells in the engrafted tissue by 46% (P < .05).


CONCLUSIONS
Preconditioning of myogenic cells with bFGF should be seriously considered as a new tool for improving cell viability after cell transfer in the treatment of urinary incontinence.
Burdzińska
Anna
A
Department of Immunology, Transplantology, and Internal Disease, Transplantation Institute, Medical University of Warsaw, Warsaw, Nowogrodzka, Poland.
Bartoszuk
Urszula
U
Orzechowski
Arkadiusz
A
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D017690:Cell Transplantation; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D014521:Urethra / Q000601:surgery*
D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D001205:Ascorbic Acid