title: Highly efficient and low-cost purification of lysozyme: a novel tris(hydroxymethyl)aminomethane immobilized affinity column.
abstract: A highly efficient and low-cost affinity chromatography strategy for lysozyme (LZM) purification is reported. Using tris(hydroxymethyl)aminomethane (Tris) as ligand and macroporous silica spheres as matrix, a novel affinity column was prepared. The high specificity, stability and repeatability of this Tris immobilized affinity column were proved by LZM separations from protein mixture solutions for 20 circles and 6 months test. LZM purified from chicken egg white on the Tris affinity column had even higher purity than the commercial standard and well-maintained activity of 8287 U/mg (activity of commercial LZM was 8171 U/mg). The efficient affinity process avoiding expensive or fragile ligand would bring advantages to the routine production of LZM from chicken egg white.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Li
authors.initials: L
authors.lastname: Quan
authors.forename: Qing
authors.initials: Q
authors.lastname: Cao
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Kean
authors.initials: K
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity / Q000191:economics* / Q000379:methods*; D004529:Egg White / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014325:Tromethamine / Q000737:chemistry*
chemical_list: D014325:Tromethamine; D009113:Muramidase