Reprogramming of human somatic cells using human and animal oocytes.
There is renewed interest in using animal oocytes to reprogram human somatic cells. Here we compare the reprogramming of human somatic nuclei using oocytes obtained from animal and human sources. Comparative analysis of gene expression in morula-stage embryos was carried out using single-embryo transcriptome amplification and global gene expression analyses. Genomic DNA fingerprinting and PCR analysis confirmed that the nuclear genome of the cloned embryos originated from the donor somatic cell. Although the human-human, human-bovine, and human-rabbit clones appeared morphologically similar and continued development to the morula stage at approximately the same rate (39, 36, and 36%, respectively), the pattern of reprogramming of the donor genome was dramatically different. In contrast to the interspecies clones, gene expression profiles of the human-human embryos showed that there was extensive reprogramming of the donor nuclei through extensive upregulation, and that the expression pattern was similar in key upregulation in normal control embryos. To account for maternal gene expression, enucleated oocyte transcriptome profiles were subtracted from the corresponding morula-stage embryo profiles. t-Test comparisons (median-normalized data @ fc>4; p<0.005) between human in vitro fertilization (IVF) embryos and human-bovine or human-rabbit interspecies somatic cell transfer (iSCNT) embryos found between 2400 and 2950 genes that were differentially expressed, the majority (60-70%) of which were downregulated, whereas the same comparison between the bovine and rabbit oocyte profiles found no differences at all. In contrast to the iSCNT embryos, expression profiles of human-human clones compared to the age-matched IVF embryos showed that nearly all of the differentially expressed genes were upregulated in the clones. Importantly, the human oocytes significantly upregulated Oct-4, Sox-2, and nanog (22-fold, 6-fold, and 12-fold, respectively), whereas the bovine and rabbit oocytes either showed no difference or a downregulation of these critical pluripotency-associated genes, effectively silencing them. Without appropriate reprogramming, these data call into question the potential use of these discordant animal oocyte sources to generate patient-specific stem cells.
Chung
Young
Y
Advanced Cell Technology, Worcester, MA 01605, USA. rlanza@advancedcell.com
Bishop
Colin E
CE
Treff
Nathan R
NR
Walker
Stephen J
SJ
Sandler
Vladislav M
VM
Becker
Sandy
S
Klimanskaya
Irina
I
Wun
Wan-Song
WS
Dunn
Randall
R
Hall
Rebecca M
RM
Su
Jing
J
Lu
Shi-Jiang
SJ
Maserati
Marc
M
Choi
Young-Ho
YH
Scott
Richard
R
Atala
Anthony
A
Dittman
Ralph
R
Lanza
Robert
R
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism*; D065150:Cellular Reprogramming; D019976:Cloning, Organism; D005260:Female; D020869:Gene Expression Profiling; D005838:Genotype; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000235:genetics; D053652:Nuclear Transfer Techniques; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D025341:Principal Component Analysis; D011817:Rabbits; D013234:Stem Cells / Q000502:physiology