Heterologous expression, crystallization and preliminary X-ray characterization of CcCel6C, a glycoside hydrolase family 6 enzyme from the basidiomycete Coprinopsis cinerea.
CcCel6C is a gene that encodes a glycoside hydrolase family 6 (GH6) enzyme in the Coprinopsis cinerea genome. In the evolutionary tree of GH6 enzymes, the encoded enzyme was closely related to Cel6B from Humicola insolens, previously called endoglucanase VI, while its amino-acid sequence revealed a region corresponding to the C-terminal active-site-enclosing loop typical of cellobiohydrolase II. Here, the crystallization of CcCel6C produced in Escherichia coli is reported. The square prismatic crystal belonged to the triclinic space group P1, with unit-cell parameters a = 44.04, b = 45.11, c = 48.90 A, alpha = 77.81, beta = 87.34, gamma = 68.79 degrees. Diffraction data were collected to 1.6 A resolution.
Kurakata
Yuma
Y
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Tonozuka
Takashi
T
Liu
Yuan
Y
Kaneko
Satoshi
S
Nishikawa
Atsushi
A
Fukuda
Kiyoharu
K
Yoshida
Makoto
M
D001487:Basidiomycota / Q000737:chemistry* / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D003302:Coprinus / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics
D006026:Glycoside Hydrolases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase