Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat.
Powdery mildew resistance from Thinopyrum intermedium was introgressed into common wheat (Triticum aestivum L.). Genetic analysis of the F(1), F(2), F(3) and BC(1) populations from powdery mildew resistant line CH5025 revealed that resistance was controlled by a single dominant allele. The gene responsible for powdery mildew resistance was mapped by the linkage analysis of a segregating F(2) population. The resistance gene was linked to five co-dominant genomic SSR markers (Xcfd233, Xwmc41, Xbarc11, Xgwm539 and Xwmc175) and their most likely order was Xcfd233-Xwmc41-Pm43-Xbarc11-Xgwm539-Xwmc175 at 2.6, 2.3, 4.2, 3.5 and 7.0 cM, respectively. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines, the polymorphic markers and the resistance gene were assigned to chromosome 2DL. As no powdery mildew resistance gene was previously assigned to chromosome 2DL, this new resistance gene was designated Pm43. Pm43, together with the identified closely linked markers, could be useful in marker-assisted selection for pyramiding powdery mildew resistance genes.
He
Runli
R
School of Life Science and Technology, Shanxi University, 030006, Taiyuan, Shanxi, China.
Chang
Zhijian
Z
Yang
Zujun
Z
Yuan
Zongying
Z
Zhan
Haixian
H
Zhang
Xiaojun
X
Liu
Jianxia
J
D001203:Ascomycota / Q000472:pathogenicity*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics*; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000382:microbiology*; D006109:Poaceae / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D011110:Polymorphism, Genetic; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
D005819:Genetic Markers