The c2 domains of human synaptotagmin 1 have distinct mechanical properties.
Synaptotagmin 1 (Syt1) is the Ca(+2) receptor for fast, synchronous vesicle fusion in neurons. Because membrane fusion is an inherently mechanical, force-driven event, Syt1 must be able to adapt to the energetics of the fusion apparatus. Syt1 contains two C2 domains (C2A and C2B) that are homologous in sequence and three-dimensional in structure; yet, a number of observations have suggested that they have distinct biochemical and biological properties. In this study, we analyzed the mechanical stability of the C2A and C2B domains of human Syt1 using single-molecule atomic force microscopy. We found that stretching the C2AB domains of Syt1 resulted in two distinct unfolding force peaks. The larger force peak of approximately 100 pN was identified as C2B and the second peak of approximately 50 pN as C2A. Furthermore, a significant fraction of C2A domains unfolded through a low force intermediate that was not observed in C2B. We conclude that these domains have different mechanical properties. We hypothesize that a relatively small stretching force may be sufficient to deform the effector-binding regions of the C2A domain and modulate the affinity for soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptors (SNAREs), phospholipids, and Ca(+2).
Fuson
Kerry L
KL
Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas 77555, USA.
Ma
Liang
L
Sutton
R Bryan
RB
Oberhauser
Andres F
AF
D001696:Biomechanical Phenomena; D002118:Calcium / Q000378:metabolism; D064211:Connectin; D006801:Humans; D008958:Models, Molecular; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011489:Protein Denaturation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D050600:SNARE Proteins / Q000378:metabolism; D050863:Synaptotagmin I / Q000737:chemistry* / Q000378:metabolism*
D064211:Connectin; D009124:Muscle Proteins; D010743:Phospholipids; D050600:SNARE Proteins; D050863:Synaptotagmin I; C577285:TTN protein, human; D011494:Protein Kinases; D002118:Calcium