title: An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli.
abstract: The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.
authors.affiliation: Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, Korea.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Won-kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Dina
authors.initials: D
authors.lastname: Rairakhwada
authors.forename: Pil-Soo
authors.initials: PS
authors.lastname: Seo
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Choi
authors.forename: Ki-Bang
authors.initials: KB
authors.lastname: Song
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Rhee
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D020638:Rahnella / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D011994:Recombinant Proteins