Kinetic properties of dromedary pancreatic lipase: a comparative study on emulsified and monomolecular substrate.
Using the classical emulsified system and the monomolecular film technique, we compared several interfacial properties of dromedary pancreatic lipase (DrPL) with those of a mammal (human) and an avian (turkey) model. Like turkey pancreatic lipase (TPL) and unlike human pancreatic lipase (HPL), in the absence of colipase and bile salts, using tributyrin emulsion or monomolecular films of dicaprin at low surface pressure, DrPL hydrolyses pure tributyrin emulsion, as well as dicaprin films maintained at low surface pressures. DrPL was also able to hydrolyse triolein emulsion in the absence of any additive and despite the accumulation of long-chain free fatty acids at the interface. The difference of behaviours between the two mammal pancreatic lipases (DrPL and HPL) can be explained by the penetration capacity of each enzyme. DrPL presents a critical surface pressure value (21 m Nm(-1)) that is more important than this of HPL. Subsequently, the dromedary pancreatic lipase interacts efficiently with interfaces and it is not denaturated at high interfacial energy. A kinetic study on the surface pressure dependency, stereospecificity and regioselectivity of DrPL was performed using optically pure stereoisomers of either three dicaprin isomers containing a single hydrolysable decanoyl ester bond that were spread as monomolecular films at the air/water interface. Interestingly, in comparison with all the previously studied mammal pancreatic lipases, DrPL presents the highest preference for adjacent ester groups of dicaprin isomers (1,2-sn-dicaprin and 2,3-sn-dicaprin) at high surface pressure. Furthermore, DrPL forms a pancreatic lipase subgroup in which the stereopreference switches from sn-3 position to the sn-1 position when increasing the surface pressure.
Jemel
Ikram
I
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, Sfax, Tunisia.
Fendri
Ahmed
A
Gargouri
Youssef
Y
Bezzine
Sofiane
S
D000818:Animals; D002162:Camelus; D004075:Diglycerides / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D000069463:Olive Oil; D010179:Pancreas / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000737:chemistry; D010938:Plant Oils / Q000378:metabolism; D011312:Pressure; D013237:Stereoisomerism; D013499:Surface Properties; D014280:Triglycerides / Q000737:chemistry
D004075:Diglycerides; D004655:Emulsions; D000069463:Olive Oil; D010713:Phosphatidylcholines; D010938:Plant Oils; D014280:Triglycerides; C044297:1,2-didecanoylglycerol; D008049:Lipase; C518354:PNLIP protein, human; C005830:tributyrin