An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli.
The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.
Seo
Jeong-Woo
JW
Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, Korea.
Hong
Won-kyung
WK
Rairakhwada
Dina
D
Seo
Pil-Soo
PS
Choi
Min Ho
MH
Song
Ki-Bang
KB
Rhee
Sang-Ki
SK
Kim
Chul Ho
CH
D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D020638:Rahnella / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic
D011994:Recombinant Proteins