title: Involvement of heme oxygenase-1 in kaempferol-induced anti-allergic actions in RBL-2H3 cells.
abstract: Kaempferol is one of the most commonly found dietary flavonoids. The exposure to kaempferol is known to inhibit degranulation from mast cells, but the inhibitory mechanism of degranulation has not been clarified yet. In this study, we investigated the involvement of heme oxygenase (HO)-1 in the anti-allergic action of kaempferol against degranulation in rat basophilic leukemia (RBL-2H3) cells. Our results demonstrate upregulation of HO enzymatic activity after short (15 min) exposure to kaempferol, followed by the induction of HO-1 expression in protein. The involvement of HO-1 in the kaempferol-induced inhibition of degranulation was confirmed using tin protoporphyrin IX (SnPP), a HO-1 inhibitor. These findings strongly suggest that kaempferol exerts anti-allergic actions via activation of the HO-1.
authors.affiliation: Department of Medical Technology, Nagoya University School of Health Science, 1-1-20 Daikou-minami, Higashi-ku, Nagoya, 461-8673, Japan.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Hirose
authors.forename: Miyoko
authors.initials: M
authors.lastname: Matsushima
authors.forename: Kenzo
authors.initials: K
authors.lastname: Takagi
authors.forename: Yui
authors.initials: Y
authors.lastname: Ota
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishigami
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hirayama
authors.forename: Yuta
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Naozumi
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Imaizumi
authors.forename: Kenji
authors.initials: K
authors.lastname: Baba
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kawabe
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D047309:Flavones; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D044949:Kaempferols / Q000276:immunology / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology; D051381:Rats; D015854:Up-Regulation / Q000235:genetics
chemical_list: D018926:Anti-Allergic Agents; D047309:Flavones; D044949:Kaempferols; C006552:kaempferol; D051547:Heme Oxygenase-1