title: Performance of the whole-blood stimulation assay for assessing innate immune activation under field conditions.
abstract: Innate propensity of immune activation is reflected in production of pro- and anti-inflammatory cytokines upon stimulation of Toll-like receptors (TLR) in whole-blood stimulation assays. The validity of the whole-blood stimulation assay under field conditions has not been evaluated extensively. Here, we have determined correlation of individually repeated whole-blood stimulation assays in a field-study in Ghana and compared it with that of two Dutch populations performed under optimal conditions. We also examined cytokine production to various TLR-agonists in order to create an assay that would mimic general innate immune responses. Under field conditions repeated assessments of lipopolysaccharide-induced Tumor Necrosis Factor-alpha (TNFalpha) production were poorly correlated (r=0.15, p=0.087). Correlation was relatively high for production of Interleukin-10 (IL10) (r=0.48, p<0.001) and comparable to that observed in the Dutch population under optimal conditions. Combined stimulation with lipopolysaccharide and zymosan resulted in cytokine production profiles that were similar to that attained after stimulation with a mixed culture of bacteria. Here, we conclude that variation of a whole-blood assay performed in field setting is large in general but that production of IL10 seems to better reflect an innate pro- or anti-inflammatory tendency whereas production of TNFalpha may predominantly reflect recent immunological challenges. Furthermore, simultaneous stimulation of several Toll-like receptors may mimic general innate immune activation.
authors.affiliation: Department of Gerontology & Geriatrics C2-R, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. l.may@lumc.nl
authors.forename: L
authors.initials: L
authors.lastname: May
authors.forename: D
authors.initials: D
authors.lastname: van Bodegom
authors.forename: M
authors.initials: M
authors.lastname: Kuningas
authors.forename: J J
authors.initials: JJ
authors.lastname: Meij
authors.forename: A J M
authors.initials: AJ
authors.lastname: de Craen
authors.forename: M
authors.initials: M
authors.lastname: Frölich
authors.forename: R G J
authors.initials: RG
authors.lastname: Westendorp
mesh_terms: D000369:Aged, 80 and over; D001769:Blood; D016207:Cytokines / Q000096:biosynthesis*; D005869:Ghana; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D016753:Interleukin-10 / Q000096:biosynthesis*; D007753:Laboratories; D008070:Lipopolysaccharides / Q000494:pharmacology; D009426:Netherlands; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*; D051193:Toll-Like Receptors / Q000819:agonists / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10