title: Protein-protein interactions in two potyviruses using the yeast two-hybrid system.
abstract: Interactions between all ten mature proteins of the potyviruses Soybean mosaic virus (Pinellia ternata isolate) and Shallot yellow stripe virus were investigated using yeast two-hybrid (Y2H) assays. Consistently strong self-interactions were found between the pairs of HC-Pro, VPg, NIa-Pro, NIb and CP in both viruses. Apart from the NIb, such interactions have been previously reported for some other potyviruses. The 6K1/NIa-Pro combination gave a consistently moderate to strong interaction in both directions for both viruses. This interaction occurred even when the 6K1 of SMV-P was truncated to eliminate the C-terminal motif that acts as a recognition site for cleavage by the NIa-Pro. Many other interactions occurred only in one direction or only for one of the two viruses. When taken together with other published reports, the data suggest that interactions detected by Y2H should be regarded as only preliminary indications.
authors.affiliation: College of Plant Protection, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Shi
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Luo
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Lu
authors.forename: Hongying
authors.initials: H
authors.lastname: Zheng
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wu
mesh_terms: D017800:Potyvirus / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins