Linking dengue virus entry and translation/replication through amphisomes.
Amphisomes are preautolysosomal vacuoles formed upon the fusion of autophagosomes with endosomes, and as such represent a critical meeting point between endocytic and autophagic pathways. Dengue virus enters into susceptible cells by clathrin-mediated endocytosis, and colocalization of dengue markers with markers of both autophagic and endosomal vesicles demonstrates that amphisomes are a site of dengue virus replication and translation. This work links for the first time the processes of dengue virus entry and translation/replication, and allows for interpretation of the early part of the dengue virus life cycle in terms of a continual association with membranes of an endosomal-autophagosomal lineage.
Panyasrivanit
Mingkwan
M
Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Nakorn Pathom, Thailand.
Khakpoor
Atefeh
A
Wikan
Nitwara
N
Smith
Duncan R
DR
D000330:Aedes / Q000821:virology; D000818:Animals; D001343:Autophagy; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D003716:Dengue Virus / Q000378:metabolism*; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D010588:Phagosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000378:metabolism; D014779:Virus Replication
D002966:Clathrin