title: Hyperbaric oxygen treatment induces platelet aggregation and protein release, without altering expression of activation molecules.
abstract: OBJECTIVE
  To investigate the effect of hyperbaric oxygen (HBO) on platelet physiology.
  
  
  METHODS
  Human platelets were exposed to HBO (97.7% O(2), balance CO(2) at 2.2 ata) or control (CON; 5% CO(2), balance air at 1 ata) for 90 min, and analyzed for aggregation, protein release, ()NO production, and activation.
  
  
  RESULTS
  HBO induced 29.8+/-3.0% of platelets to aggregate compared with CON (5.5+/-0.9%). Proteins observed to be released in greater abundance from HBO- compared with CON-treated platelets included 14-3-3 zeta and alpha-2-macroglobulin. Release of ()NO by platelets was unaffected following exposure to HBO, as was platelet activation as measured by surface expression of PECAM-1, CD62P and the activated form of alpha(IIB)beta(IIIa).
  
  
  CONCLUSIONS
  Exposure to HBO induces both platelet aggregation and protein release. Further study will better define the precise mechanisms and effects of HBO on platelet activation.
authors.affiliation: Institute of Biomedical and Clinical Science, Peninsula College of Medicine and Dentistry, University of Exeter, Exeter, UK. Frances.Shaw@manchester.ac.uk
authors.forename: Frances L
authors.initials: FL
authors.lastname: Shaw
authors.forename: Paul G
authors.initials: PG
authors.lastname: Winyard
authors.forename: Gary R
authors.initials: GR
authors.lastname: Smerdon
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Bryson
authors.forename: A John
authors.initials: AJ
authors.lastname: Moody
authors.forename: Paul
authors.initials: P
authors.lastname: Eggleton
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D001792:Blood Platelets / Q000737:chemistry / Q000502:physiology*; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D006931:Hyperbaric Oxygenation; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000096:biosynthesis; D009573:Nitrites / Q000032:analysis; D019007:P-Selectin / Q000096:biosynthesis; D015539:Platelet Activation; D010974:Platelet Aggregation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000096:biosynthesis; D053657:Platelet-Rich Plasma / Q000737:chemistry; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D001798:Blood Proteins; D008562:Membrane Glycoproteins; D009566:Nitrates; D009573:Nitrites; D019007:P-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D000511:alpha-Macroglobulins; D009569:Nitric Oxide