[Evaluation of soil lead contamination by biomarkers in leaves of Vicia faba L. seedlings].
Stress proteins (HSP70 and HSP60) and other related biological parameters were investigated in leaves of Vicia faba L. seedlings subjected to soil lead stress. The seedlings were cultivated outdoors in pots for five weeks. Results showed that total lead content, superoxide radical (O2*-) and lipid peroxidation productions in the leaves increased with the increase of extraneous lead in soils. Significant correlation was found between the O2*- and lipid peroxidation productions (r = 0.973, p < 0.01). Activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were activated. Moreover, HSP70 and HSP60 increased more sensitively than the other parameters in response to lead stress. This indicates lead might cause lipid peroxidation in the leaves by mediation of O2*-. The activated antioxidative enzymes alleviated the stress and damage of reactive oxygen species. HSP70 and HSP60 are potential biomarkers for early warning of lead-contaminated soils. Additionally, concentrations of soil lead and related biological parameters should be combined.
Wang
Cheng-Run
CR
State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210093, China. chengrunw66@yahoo.com.cn
Wang
Xiao-Rong
XR
Yu
Hong-Xia
HX
Qiao
Song
S
Zhang
Bao-Gang
BG
Sun
Jian
J
D018834:Chaperonin 60 / Q000378:metabolism; D004784:Environmental Monitoring; D005069:Evaluation Studies as Topic; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007854:Lead / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis*; D013482:Superoxide Dismutase / Q000378:metabolism; D031306:Vicia / Q000737:chemistry* / Q000378:metabolism
D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D012989:Soil Pollutants; D007854:Lead; D013482:Superoxide Dismutase