title: Assessment of pollution in the West Black Sea Coast of Turkey using biomarker responses in fish.
abstract: Aim of this study was to determine the extent of pollution in the West Black Sea Coast of Turkey by measuring CYP1A associated EROD activity, phase II enzyme, glutathione S-transferase and antioxidant enzymes, catalase and glutathione reductase activities and immunochemical detection of CYP1A protein level in the liver of mullet. The fish samples were caught from six locations having a varying degree of pollution in the West Black Sea Region of Turkey in August 2005, 2006 and 2007. Mullets caught from Zonguldak Harbour, Ereğli Harbour and Gülüç Stream's Mouth displayed 6-9-fold higher EROD, 2-4-fold higher glutathione S-transferase and 2-3-fold higher catalase activities than the reference site, Amasra. Total polyaromatic hydrocarbon levels in mullets caught from these locations were also significantly higher (2-4-fold) than Amasra. The results of this study indicate that Zonguldak Harbour, Ereğli Harbour and Gülüç Stream are highly polluted by polycyclic aromatic hydrocarbons and related contaminants.
authors.affiliation: Department of Chemistry, Abant Izzet Baysal University, Bolu, Turkey. bozcaarmutlu_a@ibu.edu.tr
authors.forename: Azra
authors.initials: A
authors.lastname: Bozcaarmutlu
authors.forename: Canan
authors.initials: C
authors.lastname: Sapmaz
authors.forename: Zuleyha
authors.initials: Z
authors.lastname: Aygun
authors.forename: Emel
authors.initials: E
authors.lastname: Arinç
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002374:Catalase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000201:enzymology*; D009792:Oceans and Seas; D023701:Smegmamorpha / Q000502:physiology*; D014421:Turkey; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002374:Catalase; D019363:Cytochrome P-450 CYP1A1; D005980:Glutathione Reductase; D005982:Glutathione Transferase