title: Engineering a uranyl-specific binding protein from NikR.
abstract: A new pick-up line: The first uranyl-selective DNA-binding protein is designed using the E. coli nickel(II)-responsive protein NikR as the template. The resulting NikR' protein binds uranyl (see picture) with a dissociation constant K(d) = 53 nM and selectively binds to DNA in the presence of uranyl.
authors.affiliation: Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
authors.forename: Seraphine V
authors.initials: SV
authors.lastname: Wegner
authors.forename: Hande
authors.initials: H
authors.lastname: Boyaci
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Mark P
authors.initials: MP
authors.lastname: Jensen
authors.forename: Chuan
authors.initials: C
authors.lastname: He
mesh_terms: D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009532:Nickel; D015202:Protein Engineering; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014501:Uranium / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D050505:Mutant Proteins; C402409:NikR protein, E coli; D012097:Repressor Proteins; D014501:Uranium; D009532:Nickel; D004247:DNA