title: Effects of silencing Id-1 in cell culture of human adenoid cystic carcinoma.
abstract: Adenoid cystic carcinoma (ACC) is a slow growing but highly invasive cancer with a high recurrence rate. Id (inhibitor of DNA binding) proteins are dominant regulators of basic helix-loop-helix transcription factors that control malignant cell behavior in many different tissues. This study aimed to identify the potential role of inhibiting DNA binding-1 (Id-1) in human salivary adenoid cystic carcinoma (SACC) progression. First, we compared the Id-1 protein expression in a human salivary adenoid cystic carcinoma cell line (ACCM) against three other cell lines and found that Id-1 protein expression in ACCM to be significantly higher. Then we measured Id-1 mRNA and protein expression in ACCM before and after RNA interference (RNAi), which showed successful inhibition of Id-1. Further studies then demonstrated that the proliferation and invasiveness of ACCM cells were dramatically down-regulated, and increased numbers of apoptotic cells were detected after Id-1 silencing. Consequently, our data suggest that Id-1 is a potential target in the treatment of human salivary adenoid cystic carcinoma.
authors.affiliation: Department of Plastic Surgery, Qilu Hospital, Shandong University, Jinan, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Liu
authors.forename: Hongshun
authors.initials: H
authors.lastname: Qi
authors.forename: Yutang
authors.initials: Y
authors.lastname: Li
authors.forename: Haixian
authors.initials: H
authors.lastname: He
authors.forename: Fengcai
authors.initials: F
authors.lastname: Wei
mesh_terms: D017209:Apoptosis; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D001324:Autoantigens; D003598:Cytoskeletal Proteins; C103926:LMOD1 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger