PDLIM4, an actin binding protein, suppresses prostate cancer cell growth.
We investigated the molecular function of PDLIM4 in prostate cancer cells. PDLIM4 mRNA and protein-expression levels were reduced in LNCaP, LAPC4, DU145, CWR22, and PC3 prostate cancer cells. The re-expression of PDLIM4 in prostate cancer cells has significantly reduced the cell growth and clonogenicity with G1 phase of cell-cycle arrest. We have shown the direct interaction of PDLIM4 with F-actin. Restoration of PDLIM4 expression resulted in reduction of tumor growth in xenografts. These results suggest that PDLIM4 may function as a tumor suppressor, involved in the control of cell proliferation by associating with actin in prostate cancer cells.
Vanaja
Donkena Krishna
DK
Department of Urology, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN 55905, USA.
Grossmann
Michael E
ME
Cheville
John C
JC
Gazi
Mozammel H
MH
Gong
Aiyu
A
Zhang
Jin San
JS
Ajtai
Katalin
K
Burghardt
Thomas P
TP
Young
Charles Y F
CY
D000199:Actins / Q000032:analysis; D000818:Animals; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D060588:LIM Domain Proteins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins; D011471:Prostatic Neoplasms / Q000473:pathology / Q000517:prevention & control*; D025521:Tumor Suppressor Proteins / Q000502:physiology*
D000199:Actins; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; D008840:Microfilament Proteins; C486247:PDLIM4 protein, human; C486248:Pdlim4 protein, mouse; D025521:Tumor Suppressor Proteins