Photosensitizer-loaded magnetic nanoemulsion for use in synergic photodynamic and magnetohyperthermia therapies of neoplastic cells.
In this study a magnetic nanoemulsion (MNE) was developed from a mixture of two components, namely biodegradable surfactants and biocompatible citrate-coated cobalt ferrite-based magnetic fluid, for entrapment of Zn(II)-Phthalocyanine (ZnPc), the latter a classical photosensitizer (PS) species used in photodynamic therapy (PDT) procedures. The sample's stability was evaluated as a function of time using photocorrelation spectroscopy (PCS) for determination of the average hydrodynamic diameter, diameter dispersion and zeta potential. The ZnPc-loaded magneto nanoemulstion (ZnPc/MNE) formulation was evaluated in vitro assays to access the phototoxicity and the effect of application of AC magnetic fields (magnetohyperthermia damage) after incubation with J774-A1 macrophages cells. Darkness toxicity, phototoxicity and AC magnetic field exposures revealed an enhancement response for combined photodynamic and magnetohyperthermia (MHT) processes, indicating the presence of the synergic effect.
Primo
Fernando L
FL
Departamento de Química, Laboratório de Fotobiologia e Fotomedicína, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901, Ribeirão Preto-SP, Brasil.
Rodrigues
Marcilene M A
MM
Simioni
Andreza Ribeiro
AR
Lacava
Zulmira G M
ZG
Morais
Paulo C
PC
Tedesco
Antonio C
AC
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions; D006979:Hyperthermia, Induced / Q000379:methods*; D008264:Macrophages / Q000187:drug effects* / Q000528:radiation effects; D008280:Magnetics / Q000379:methods*; D008422:Materials Testing; D051379:Mice; D050997:Nanomedicine / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry / Q000627:therapeutic use*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry
D004337:Drug Carriers; D004655:Emulsions; D017319:Photosensitizing Agents