title: Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. coli methionine repressor, MetJ, for its operator DNA.
abstract: The efficient synthesis of a range of stable SAM mimetics, and their ability to promote the binding of the E. coli methionine repressor (MetJ) to its operator DNA, is described.
authors.affiliation: School of Chemistry, University of Leeds, Leeds, UKLS2 9JT, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Joce
authors.forename: Jamie
authors.initials: J
authors.lastname: Caryl
authors.forename: Peter G
authors.initials: PG
authors.lastname: Stockley
authors.forename: Stuart
authors.initials: S
authors.lastname: Warriner
authors.forename: Adam
authors.initials: A
authors.lastname: Nelson
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008024:Ligands; D011485:Protein Binding / Q000187:drug effects; D012097:Repressor Proteins / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000031:analogs & derivatives* / Q000138:chemical synthesis
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008024:Ligands; D012097:Repressor Proteins; C060486:methionine repressor protein, Bacteria; D012436:S-Adenosylmethionine; D004247:DNA