title: Adhesion of human probiotic Lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens.
abstract: OBJECTIVE
  The ability of a probiotic Lactobacillus rhamnosus strain (Lcr35) to adhere to cervical and vaginal cells and to affect the viability of two main vaginosis-associated pathogens, Prevotella bivia, Gardnerella vaginalis, as well as Candida albicans was investigated.
  
  
  METHODS
  Adhesion ability was determined in vitro with immortalized epithelial cells from the endocervix, ectocervix, and vagina. Coculture experiments were performed to count viable pathogens cells in the presence of Lcr35.
  
  
  RESULTS
  Lcr35 was able to specifically and rapidly adhere to the three cell lines. In coculture assays, a decrease in pathogen cell division rate was observed as from 4 hours of incubation and bactericidal activity after a longer period of incubation, mostly with P. bivia.
  
  
  CONCLUSIONS
  The ability of Lcr35 to adhere to cervicovaginal cells and its antagonist activities against vaginosis-associated pathogens suggest that this probiotic strain is a promising candidate for use in therapy.
authors.affiliation: Laboratory of Bacteriology, UFR Pharmacy, University Clermont 1, Clermont-Ferrand, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Coudeyras
authors.forename: Gwendoline
authors.initials: G
authors.lastname: Jugie
authors.forename: Marion
authors.initials: M
authors.lastname: Vermerie
authors.forename: Christiane
authors.initials: C
authors.lastname: Forestier
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion / Q000502:physiology*; D002176:Candida albicans / Q000254:growth & development* / Q000472:pathogenicity; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D006194:Gardnerella vaginalis / Q000254:growth & development* / Q000472:pathogenicity; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000502:physiology*; D018720:Prevotella / Q000254:growth & development* / Q000472:pathogenicity; D019936:Probiotics; D014621:Vagina / Q000382:microbiology; D016585:Vaginosis, Bacterial / Q000382:microbiology* / Q000517:prevention & control