Helicobacter pylori enhances cyclooxygenase 2 expression via p38MAPK/ATF-2 signaling pathway in MKN45 cells.
The over-expression of COX-2 (Cyclooxygenase 2) protein has been reported to play a key role in the incidence and development of Helicobacter pylori-associated gastric cancer. The induction of COX-2 in the gastric cancer cells with H. pylori has been demonstrated previously, but little is known about the mechanism. This study reported that the COX-2 mRNA and proteins expression level and the activity of COX-2 promoter increased remarkably with H. pylori stimulation in the MKN45 gastric cancer cells. H. pylori also stimulated phosphorylation of p38MAPK and ATF-2, which is the downstream kinase of p38MAPK. Moreover, the expression levels of COX-2 were suppressed with p38MAPK inhibitor treatment. These results suggest that H. pylori-induced activation of p38MAPK/ATF-2-mediated signal pathway is necessary for the expression of COX-2.
Li
Qi
Q
Department of Oncology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
Liu
Ningning
N
Shen
Bo
B
Zhou
Lihong
L
Wang
Yan
Y
Wang
Yiqin
Y
Sun
Jue
J
Fan
Zhongze
Z
Liu
Rui Hai
RH
D051697:Activating Transcription Factor 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D016481:Helicobacter Infections / Q000150:complications; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D010766:Phosphorylation; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D051697:Activating Transcription Factor 1; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases