title: Bladder tumor infiltrating mature dendritic cells and macrophages as predictors of response to bacillus Calmette-Guérin immunotherapy.
abstract: BACKGROUND
  The clinical significance of tumor-infiltrating dendritic cells (TIDCs) and tumor-associated macrophages (TAMs) as markers of immune response has been reported for many cancers.
  
  
  OBJECTIVE
  To measure tumor infiltration by CD83(+) dendritic cells (DCs) and CD68(+) macrophages in non-muscle-invasive urothelial cancer (NMIUC) prior to bacillus Calmette-Guérin (BCG) immunotherapy and to evaluate their significance in the response to immunotherapy.
  
  
  METHODS
  Patients with NMIUC at high risk of recurrence and progression were recruited for a study on markers of the response to BCG.
  
  
  METHODS
  Patients were treated by transurethral resection followed by maintenance BCG.
  
  
  METHODS
  Immunohistochemical staining with anti-CD83 and anti-CD68 monoclonal antibodies on 53 and 46 NMIUC tumors, respectively, prior to BCG treatment. A scoring index was calculated based on the average density of positive cells within the papillary axis, the stroma, lymphoid aggregates, and infiltration into tumors.
  
  
  CONCLUSIONS
  CD83(+) TIDCs were observed mostly within lymphoid aggregates. Multivariate Cox regression analysis showed that maintenance BCG (more than one maintenance cycle) was highly effective in patients with a low level of CD83(+) TIDCs at time of resection (hazard ratio [HR]: 0.035; p=0.002) but showed reduced efficacy in patients with a high level of CD83(+) TIDCs (HR: 0.87; p=0.810). A high level of infiltration by CD83(+) TIDCs slightly decreased the risk of recurrence in patients treated with one or no maintenance BCG cycle (HR: 0.4; p=0.117). In the same population, a strong infiltration of CD68(+) TAMs was associated with an increased risk of recurrence (HR: 3.8; p=0.013).
  
  
  CONCLUSIONS
  These results suggest that patients with a high level of infiltration by CD83(+) TIDCs or CD68(+) TAMs do not respond as well to BCG immunotherapy. If confirmed in larger cohorts, the pretreatment level of infiltration by these cells may be useful to influence the choice of treatment strategy.
authors.affiliation: Centre de recherche en cancérologie de l'Université Laval, L'Hôtel-Dieu de Québec, CHUQ, Québec, Canada.
authors.forename: Cherifa
authors.initials: C
authors.lastname: Ayari
authors.forename: Hélène
authors.initials: H
authors.lastname: LaRue
authors.forename: Hélène
authors.initials: H
authors.lastname: Hovington
authors.forename: Marc
authors.initials: M
authors.lastname: Decobert
authors.forename: François
authors.initials: F
authors.lastname: Harel
authors.forename: Alain
authors.initials: A
authors.lastname: Bergeron
authors.forename: Bernard
authors.initials: B
authors.lastname: Têtu
authors.forename: Louis
authors.initials: L
authors.lastname: Lacombe
authors.forename: Yves
authors.initials: Y
authors.lastname: Fradet
mesh_terms: D000283:Administration, Intravesical; D000368:Aged; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000378:metabolism*; D001500:BCG Vaccine / Q000627:therapeutic use*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy* / Q000276:immunology / Q000401:mortality / Q000473:pathology; D015331:Cohort Studies; D015653:Cystectomy / Q000379:methods; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy / Q000379:methods; D053208:Kaplan-Meier Estimate; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011336:Probability; D011379:Prognosis; D018570:Risk Assessment; D016019:Survival Analysis; D016896:Treatment Outcome; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000401:mortality / Q000473:pathology; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001500:BCG Vaccine; D014408:Biomarkers, Tumor; C067980:CD68 antigen, human; D000097382:CD68 Molecule