Simultaneous determination of bioactive xanthone glycosides and norlignans from ethanolic extract of Anemarrhena asphodeloides by liquid chromatography.
The rhizomes of Anemarrhena asphodeloides Bunge (Liliaceae) are prescribed as crude drugs in herbal medication for the treatment of various diseases such as diabetes, inflammation, and platelet aggregation inhibition. A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed to study the quantitative determination of 5 bioactive compounds from these rhizomes, namely, neomangiferin, mangiferin, isomangiferin, nyasol, and methylnyasol. Chromatographic analysis was performed on Capcell Pak C18 column (150 x 4.6 mm, 3 microm) with a mobile phase consisting of acetonitrile, methanol, and 0.1% formic acid at a flow rate of 1.00 mL/min. Quantitation was performed using a UV-visible detector at 260 nm. The method for the determination of reported medicinal agents was accurate and reproducible. Excellent linear behavior was observed over the investigated concentration range of 2.5-100.0 microg/mL for neomangiferin; 1.5-60.0 microg/mL for mangiferin; 0.5-20.0 microg/mL for nyasol; and 0.2-20.0 microg/mL for methylnyasol; correlation coefficient > 0.99. The intraday and interday precision over the concentration range of compounds was < 6.6% (relative standard deviation) and accuracy was between 94.9 and 109.3%. This method can be successfully applied for the analysis of medicinal compounds from the ethanolic extract of A. asphodeloides Bunge.
Islam
M Nurul
MN
Korea Institute of Science and Technology, Doping Control Center, PO Box 131, Chungryang, Seoul 130-650, Korea.
Yoo
Hye Hyun
HH
Lee
Jun
J
Nam
Joo Won
JW
Seo
Eun Kyoung
EK
Jin
Changbae
C
Kim
Dong-Hyun
DH
D031403:Anemarrhena / Q000737:chemistry*; D000998:Antiviral Agents / Q000032:analysis / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006027:Glycosides / Q000032:analysis; D017705:Lignans / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D044004:Xanthones / Q000032:analysis* / Q000302:isolation & purification
D000998:Antiviral Agents; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; D012997:Solvents; D044004:Xanthones; C061492:isomangiferin; D000431:Ethanol