title: Effect of physical conditions and chemicals on the binding of a mini-CbpA from Clostridium cellulovorans to a semi-crystalline cellulose ligand.
abstract: OBJECTIVE
  To investigate the effect that environmental factors have on Clostridium cellulovorans cellulose binding domain (CBD) binding to a semi-crystalline cellulose ligand, namely Avicel.
  
  
  RESULTS
  The behaviour of a 58 kDa mini-CbpA protein containing the CBD from the scaffoldin protein of C. cellulovorans was studied in the presence of various environmental factors, in order to determine whether such factors promote or reduce CBD binding to its ligand, thus potentially affecting its activity on the substrate. The amount of binding was found to be dependent on the Avicel concentration and optimal binding occurred when the ligand concentration was 15 mg ml(-1). Optimal CBD binding occurred at pH 7.0 and at an incubation temperature of 28 degrees C. The effects of dithiothreitol (DTT), 2-mercaptoethanol, acetone, butanol, ethanol and butyric acid were also investigated.
  
  
  CONCLUSIONS
  Temperature, pH, DTT, 2-mercaptoethanol and solvents were shown to affect the binding of C. cellulovorans CBD to Avicel.
  
  
  CONCLUSIONS
  Clostridium cellulovorans CBD binding to Avicel is affected by physical conditions and chemicals.
authors.affiliation: Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown, South Africa.
authors.forename: L M
authors.initials: LM
authors.lastname: Lavan
authors.forename: J S
authors.initials: JS
authors.lastname: Van Dyk
authors.forename: H
authors.initials: H
authors.lastname: Chan
authors.forename: R H
authors.initials: RH
authors.lastname: Doi
authors.forename: B I
authors.initials: BI
authors.lastname: Pletschke
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D046974:Clostridium cellulovorans / Q000201:enzymology / Q000378:metabolism*; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008623:Mercaptoethanol; D012997:Solvents; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D008024:Ligands; D012997:Solvents; C074074:cellulose binding protein A, Clostridium cellulovorans; D008623:Mercaptoethanol; D002482:Cellulose