Optimization of preservation conditions of As (III) bioreporter bacteria.
Long-term preservation of bioreporter bacteria is essential for the functioning of cell-based detection devices, particularly when field application, e.g., in developing countries, is intended. We varied the culture conditions (i.e., the NaCl content of the medium), storage protection media, and preservation methods (vacuum drying vs. encapsulation gels remaining hydrated) in order to achieve optimal preservation of the activity of As (III) bioreporter bacteria during up to 12 weeks of storage at 4 degrees C. The presence of 2% sodium chloride during the cultivation improved the response intensity of some bioreporters upon reconstitution, particularly of those that had been dried and stored in the presence of sucrose or trehalose and 10% gelatin. The most satisfying, stable response to arsenite after 12 weeks storage was obtained with cells that had been dried in the presence of 34% trehalose and 1.5% polyvinylpyrrolidone. Amendments of peptone, meat extract, sodium ascorbate, and sodium glutamate preserved the bioreporter activity only for the first 2 weeks, but not during long-term storage. Only short-term stability was also achieved when bioreporter bacteria were encapsulated in gels remaining hydrated during storage.
Kuppardt
Anke
A
Department Environmental Microbiology, Helmholtz Centre for Environmental Research, UFZ, Permoserstrasse 15, 04318 Leipzig, Germany.
Chatzinotas
Antonis
A
Breuer
Uta
U
van der Meer
Jan Roelof
JR
Harms
Hauke
H
D018053:Arsenites / Q000378:metabolism*; D015169:Colony Count, Microbial; D003451:Cryoprotective Agents / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D011309:Preservation, Biological / Q000379:methods*
D018053:Arsenites; D003451:Cryoprotective Agents; D003470:Culture Media; C015001:arsenite