The enamel softening and loss during early erosion studied by AFM, SEM and nanoindentation.
Enamel dissolution occurs when it contacts with acids produced by plaque bacteria, foods or drinks. There have been numerous and varied studies quantifying and characterizing the rate, extent and chemical aspects of enamel erosion; however, there is still hot debate about the amounts of enamel softening and loss. The objective of this study was to measure the enamel erosion process with high accuracy. Native third molars were partly eroded in citric acid (pH = 3.8) with some domains protected by a Ti coating layer. The surface morphology and structures before and after exposure to citric acid for different time periods were studied by AFM, SEM and nanoindentation, respectively. Based on this, the functions between the amounts of enamel softening, loss and erosion time were established for the first time. It was demonstrated that AFM, SEM and nanoindentation were suitable for measuring the early stages of enamel demineralization qualitatively and quantitatively.
Cheng
Zhen-Jiang
ZJ
Laboratory of Advanced Materials, State Key Laboratory of New Ceramic & Fine Processing, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Wang
Xiu-Mei
XM
Cui
Fu-Zhai
FZ
Ge
Jun
J
Yan
Jian-Xin
JX
D019343:Citric Acid / Q000737:chemistry*; D003743:Dental Enamel / Q000737:chemistry* / Q000503:physiopathology* / Q000648:ultrastructure; D006245:Hardness Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third / Q000737:chemistry* / Q000503:physiopathology* / Q000648:ultrastructure; D014077:Tooth Erosion / Q000473:pathology / Q000503:physiopathology*
D019343:Citric Acid