Tropicimonas isoalkanivorans gen. nov., sp. nov., a branched-alkane-degrading bacterium isolated from Semarang Port in Indonesia.
An aerobic, Gram-negative, motile bacterium, strain B51(T), was isolated from seawater obtained from Semarang Port in Indonesia. Cells of strain B51(T) were peritrichously flagellated and rod-shaped. Strain B51(T) was able to degrade alkanes, branched alkanes and alkylnaphthalenes. 16S rRNA gene sequence analysis revealed that strain B51(T) was affiliated with the family Rhodobacteraceae, and was related most closely to Thioclava pacifica TL 2(T) (94.6 % similarity). The DNA G+C content of strain B51(T) was 66.5 mol%. The major cellular fatty acids were C(18 : 1)omega7c (84.9 %), C(18 : 1)omega9c (13.8 %), C(16 : 0) (8.7 %), C(18 : 0) (6.4 %) and anteiso-C(15 : 0) (5.8 %) and the major quinone was ubiquinone-10. Based on its phenotypic and phylogenetic characteristics, strain B51(T) is considered to represent a novel species of a new genus, for which the name Tropicimonas isoalcanivorans gen. nov., sp. nov. is proposed. The type strain of the type species is B51(T) (=JCM 14837(T)=DSM 19548(T)).
Harwati
Theresia Umi
TU
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Kasai
Yuki
Y
Kodama
Yumiko
Y
Susilaningsih
Dwi
D
Watanabe
Kazuya
K
D000473:Alkanes / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012623:Seawater / Q000382:microbiology*; D013045:Species Specificity
D000473:Alkanes; D012336:RNA, Ribosomal, 16S