Efficiency of slightly acidic electrolyzed water for inactivation of Salmonella enteritidis and its contaminated shell eggs.
The efficiency of slightly acidic electrolyzed water (SAEW) at different temperatures (4, 20 and 45 degrees C) for inactivation of Salmonella enteritidis and it on the surface of shell eggs was evaluated. The bactericidal activity of SAEW, sodium hypochlorite solution (NaClO) and acidic electrolyzed water (AEW) to inactivate S. enteritidis was also compared. SAEW with a pH value of 6.0-6.5 used was generated by the electrolysis of a dilute hydrochloric acid (2.4 mM) in a chamber without a membrane. Although the pH value of SAEW was greatly higher than that of AEW (pH2.6-2.7), SAEW had a comparative powerful bactericidal activity at the same available chlorine concentrations. The efficiency of SAEW for inactivation of pure S. enteritidis cultures increased with increasing the available chlorine concentration and treatment time at the three different temperatures. The S. enteritidis counts decreased to less than 1.0 log(10) CFU/ml at available chlorine of 2 mg/l and 100% inactivation (reduction of 8.2 log(10) CFU/ml) was resulted in using SAEW with available chlorine more than 4 mg/l at 4, 20 and 45 degrees C after 2 min treatment, whereas no reduction was observed in the control samples. Moreover, SAEW was also effective for inactivating the S. enteritidis inoculated on the surface of shell eggs. A reduction of 6.5 log(10) CFU/g of S. enteritidis on shell eggs was achieved by SAEW containing 15 mg/l available chlorine for 3 min, but only a reduction of 0.9-1.2 log(10) CFU/g for the control samples. No survival of S. enteritidis was recovered in waste wash SAEW after treatment. The findings of this study indicate that SAEW may be a promising disinfectant agent for the shell egg washing processing without environmental pollution.
Cao
Wei
W
China Agricultural University, Beijing, PR China.
Zhu
Zhi Wei
ZW
Shi
Zheng Xiang
ZX
Wang
Chao Yuan
CY
Li
Bao Ming
BM
D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000517:prevention & control*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D010063:Ovum / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000187:drug effects*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013696:Temperature
D004202:Disinfectants; C118085:superoxidized water; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite