Enzymatic synthesis of peptides on a solid support.
We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.
Haddoub
Rose
R
Manchester Interdisciplinary Biocentre, The University of Manchester and School of Chemistry, 131 Princess Street, Manchester, UK M1 7DN.
Dauner
Martin
M
Stefanowicz
Fiona A
FA
Barattini
Valeria
V
Laurent
Nicolas
N
Flitsch
Sabine L
SL
D000180:Acrylic Resins; D000596:Amino Acids / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D006031:Glycosylation; D008722:Methods; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols; D014867:Water
D000180:Acrylic Resins; D000596:Amino Acids; D004798:Enzymes; D010455:Peptides; C520358:poly(acryloyl-bis(aminopropyl)polyethylene glycol); D014867:Water; D011092:Polyethylene Glycols; D010447:Peptide Hydrolases