title: The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation.
abstract: OBJECTIVE
  Platelet-rich plasma is used to deliver growth factors, in a safe and convenient manner, for enhancing bone and periodontal regeneration. However, conflicting reports regarding its effectiveness suggest that further study of the relevant cellular mechanisms is required. The aim of this study was to investigate the in vitro effect of platelet-rich plasma on osteoblasts and periodontal ligament cell function.
  
  
  METHODS
  Various concentrations of platelet-rich plasma (100, 50, 20 and 10%) and platelet-poor plasma, obtained from human donors, were applied to primary cultures of human osteoblasts and periodontal ligament cells. [(3)H]-Thymidine incorporation, crystal violet staining and MTT assays were utilized to assess DNA synthesis and proliferation. Migration was determined by assessing the cell response to a concentration gradient, while differentiation was assessed using Alazarin Red staining.
  
  
  RESULTS
  Platelet-rich plasma and platelet-poor plasma had stimulatory effects on the migration of both human osteoblasts and periodontal ligament cells. At 24 h, DNA synthesis was suppressed by the application of the various concentrations of platelet-rich plasma, but over a 5-d period, a beneficial effect on proliferation was observed, especially in response to 50% platelet-rich plasma. Platelet-poor plasma resulted in the greatest enhancement of cellular proliferation for both cell types. At a concentration of 50%, platelet-rich plasma and platelet-poor plasma facilitated differentiation of both cell types.
  
  
  CONCLUSIONS
  Platelet-rich plasma can exert a positive effect on osteoblast and periodontal ligament cell function, but this effect is concentration specific with maximal concentrations not necessarily resulting in optimal outcomes. Platelet-poor plasma also appears to have the ability to promote wound healing-associated cell function.
authors.affiliation: School of Dentistry, The University of Queensland, Queensland, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Creeper
authors.forename: A M
authors.initials: AM
authors.lastname: Lichanska
authors.forename: R I
authors.initials: RI
authors.lastname: Marshall
authors.forename: G J
authors.initials: GJ
authors.lastname: Seymour
authors.forename: S
authors.initials: S
authors.lastname: Ivanovski
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D010949:Plasma; D053657:Platelet-Rich Plasma
chemical_list: D006133:Growth Substances; D004247:DNA