title: Cellular internalization and in vivo tracking of thermosensitive luminescent micelles based on luminescent lanthanide chelate.
abstract: An amphiphilic tris(dibenzoylmethanato)europium(III) (Eu(DBM)(3)) coordinated P(MMA-co-EIPPMMA)-co-P(NIPAAm-co-NDAPM) copolymer was synthesized, which exhibited good biocompatibility and emitted strong red luminescence (MMA, methyl methacrylate; EIPPMMA, 4-(1-ethyl-1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenyl methacrylate; NIPAAm, N-isopropylacrylamide; NDAPM, (N-(3-dimethylamino)propyl)methacrylamide). The copolymer could self-assemble into micelles of size around 260 nm, and the micelles were thermosensitive at around body temperature. The drug-loaded micelles showed thermosensitive controlled drug release, and the paclitaxel loaded micelles were capable of being internalized into the tumor cells (A549) and exhibited obvious inhibition to the growth of A549 cells. Importantly, in vivo study showed the self-assembled micelles of Eu(DBM)(3) coordinated P(MMA-co-EIPPMMA)-co-P(NIPAAm-co-NDAPM) copolymer uptaken by the larvae of zebrafish could be easily tracked and be eliminated from the body within several days.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Yong-Yong
authors.initials: YY
authors.lastname: Li
authors.forename: Han
authors.initials: H
authors.lastname: Cheng
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.forename: Jing-Ling
authors.initials: JL
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Ke-Li
authors.initials: KL
authors.lastname: Zhang
authors.forename: Si-Xue
authors.initials: SX
authors.lastname: Cheng
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry*; D004337:Drug Carriers / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008823:Micelles; D009942:Organometallic Compounds / Q000737:chemistry; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013500:Surface Tension; D013696:Temperature; D015027:Zebrafish / Q000196:embryology
chemical_list: D002614:Chelating Agents; D004337:Drug Carriers; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D008823:Micelles; D009942:Organometallic Compounds; D011108:Polymers; D017239:Paclitaxel