[Relationship of myeloid differentiation-2 gene promoter polymorphisms with susceptivity of complications after severe trauma in Chinese Han population].
OBJECTIVE
To investigate the polymorphisms of myeloid differentiation-2 (MD-2) gene promoters, and to explore whether such polymorphisms are associated with the susceptibility to multiple organ dysfunction syndrome (MODS) and sepsis in Chinese Han population.


METHODS
Using polymerase chain reaction-restriction fragment length polymorphism method, the authors detected the single nucleotide polymorphisms of the promoter region of MD-2 gene at position - 1625C/G in 105 severe trauma patients (42 with sepsis). The organ function was scored.


RESULTS
The frequency of CC genotype in MD-2 gene promoter region at position - 1625 was 0.5 (21/42) in septic patients and 0.7 (44/63) in non-septic patients. The frequency of CG genotype was 0.38 (16/42) in septic patients and 0.27 (17/63) in non-septic patients. The frequency of GG genotype was 0.12 (5/42) in septic patients and 0.03 (2/63) in non-septic patients. The MODS scores in trauma patients carrying G allele at position - 1625 were significantly higher than those carrying C allele (P<0.001 for dominant effect, and P>0.05 for recessive effect). Moreover, trauma patients carrying G allele appeared to have higher risk of sepsis comparing to those carrying C allele (OR 0.477, 95% CI 0.266-0.855, P<0.05). Sepsis morbidity was significantly different between subjects with C and G alleles (P<0.05 for dominant effect, P>0.05 for recessive effect).


CONCLUSIONS
The polymorphisms of the promoter region of MD-2 gene at position - 1625 C/G is correlated with MODS and sepsis after severe trauma in Chinese Han population. The people with - 1625 G allele in the promoter region of MD-2 gene may be a risk factor of severe complications.
Gu
Wei
W
Department Four of State Key Laboratory of Trauma, Bums and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Shan
You-an
YA
Liu
Qing
Q
Zhou
Jian
J
Jiang
Dong-po
DP
Yao
Yuan-zhang
YZ
Zhang
Lian-yang
LY
Du
Ding-yuan
DY
Gao
Jin-mou
JM
Dong
Hong
H
Yang
Ce
C
Zhu
Pei-fang
PF
Wang
Zheng-guo
ZG
Jiang
Jian-xin
JX
D044466:Asian People; D002681:China; D020022:Genetic Predisposition to Disease; D006801:Humans; D051218:Lymphocyte Antigen 96 / Q000235:genetics*; D009102:Multiple Organ Failure / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D018805:Sepsis / Q000209:etiology / Q000235:genetics*; D014947:Wounds and Injuries / Q000150:complications / Q000235:genetics*
C495377:LY96 protein, human; D051218:Lymphocyte Antigen 96