title: Complete sequence of biosynthetic gene cluster responsible for producing triostin A and evaluation of quinomycin-type antibiotics from Streptomyces triostinicus.
abstract: Streptomyces triostinicus produces triostin A, an antitumor antibiotic, as its major secondary metabolite. Surprisingly, this strain also produced a trace amount of echinomycin. We sequenced the entire triostin A biosynthetic gene cluster from S. triostinicus, and found that this 36 kilobase-long gene cluster contained an ORF homologous to ecm18 that encodes a thioacetal-forming enzyme responsible for the triostin A-to-echinomycin bioconversion. These findings indicate that, unlike previously thought, S. triostinicus is capable of producing not only triostin A but also echinomycin. Our observation suggests potential value in careful re-analysis for metabolites from previously characterized natural product producers with the current technologies.
authors.affiliation: Dept. Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA.
authors.forename: Alex P
authors.initials: AP
authors.lastname: Praseuth
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kinya
authors.initials: K
authors.lastname: Hotta
authors.forename: Hiroki
authors.initials: H
authors.lastname: Oguri
authors.forename: Hideaki
authors.initials: H
authors.lastname: Oikawa
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001483:Base Sequence; D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D004448:Echinomycin / Q000096:biosynthesis* / Q000737:chemistry; D015698:Genomic Library; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011810:Quinoxalines / Q000737:chemistry / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D011810:Quinoxalines; C031945:triostin A; D004448:Echinomycin