title: Detection of marine pathogenic bacterial Vibrio species by multiplex polymerase chain reaction (PCR).
abstract: Polymerase chain reactions (PCR) were performed to accomplish quick and accurate detection of Vibrio species. The primers prepared with 16S-23S rDNA intergenic spacer (IGS) region exhibited an excellent species-specificity for Vibrio sp and detected Vibrio sp more successfully than the conventional culture method. Multiplex PCR was also fruitful not only forthe identification of the 5 standard Vibrio sp simultaneously but also for the detection of Vibrio spin the samples collected from the natural environment.
authors.affiliation: Department of Biology, Sangmyung University, Seoul-110-743, Korea. mnkim@smu.ac.kr
authors.forename: Mal Nam
authors.initials: MN
authors.lastname: Kim
authors.forename: Hyo Joo
authors.initials: HJ
authors.lastname: Bang
mesh_terms: D008386:Marine Biology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014733:Vibrio / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S