[Expression of aspartyl-(asparaginyl) beta-hydroxylase in villi in patients with missed abortion].
OBJECTIVE
To determine the difference in aspartyl-(asparaginyl) beta-hydroxylase (AAH) expression level in villi between patients with missed abortion and normal women with early pregnancy, and to confirm the expression loci of AAH in villi.


METHODS
A total of 50 patients of missed abortion were collected and categorized into a test group, which was subdivided into Group 1 and Group 2. Patients in Group 1 (n=20) were of confirmed etiological disorders while those in Group 2 (n=30) showed no obviously etiological clues. In addition, 20 women of early pregnancy with artificial abortion were categorized into a control group, whose embryos were sonographically confirmed alive before surgery. The 50 patients of missed abortion were also subdivided into a group within 4 weeks and a group over 4 weeks according to the time that the embryo stayed in utrine after death. Immunohistochemical technique and computer image analysis were used to detect the expression loci and the level of AAH in villi.


RESULTS
AAH was expressed in the endochylema and nucleus of trephocyte both in missed abortion and normal early pregnancy. The expression level of AAH in villi of missed abortion was much lower than that of in villi of normal early pregnancy (P<0.05). The expression level had no difference between different groups of patients with missed abortion(P>0.05).


CONCLUSIONS
Low expression of AAH in the endochylema and nucleus of trephocyte may play a role in patients with missed abortion.
Mi
Chunmei
C
Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhou
Changju
C
Xue
Min
M
D000030:Abortion, Missed / Q000201:enzymology*; D000328:Adult; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000201:enzymology*; D005260:Female; D006801:Humans; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy
D006899:Mixed Function Oxygenases; C058291:aspartic acid 2-oxoglutarate-dependent dioxygenase