title: Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction.
abstract: In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Hazra
authors.forename: Abhishek
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D019759:Carbon-Nitrogen Lyases / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D013831:Thiamine / Q000096:biosynthesis*; D013844:Thiazoles / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013844:Thiazoles; D019759:Carbon-Nitrogen Lyases; C551808:thiamin thiazole synthase, Bacillus subtilis; D013831:Thiamine