title: Evaluation of coronary endothelial function by catheter-type NO sensor in high-fat-diet-induced obese dogs.
abstract: BACKGROUND
  Direct measurement of plasma nitric oxide (NO) concentration is possible with a newly developed catheter-type sensor.
  
  
  RESULTS
  Adult mongrel dogs (n=5) were fed a high-fat diet (120 kcal . kg(-1) . day(-1)) for 8 months, then endothelial function was assessed by the change in NO concentration induced by acetylcholine (ACh) (DeltaNO). Simultaneously, average peak velocity (APV) was obtained by Doppler guide wire. Although fasting plasma glucose levels did not change after high-fat diet, fasting plasma insulin levels increased significantly (103+/-36 vs 106+/-27 mg/dl, P=0.89 and 0.15+/-0.15 vs 0.26+/-0.07 ng/ml, P=0.04, respectively). ACh-induced peak APV after high-fat feeding was not significantly different from that at baseline (ACh 0.1 microg/kg; 43+/-17 vs 51+/-7 cm/s, P=NS, ACh 0.4 microg/kg; 45+/-20 vs 47+/-16 cm/s, P=NS, respectively). The DeltaNO was significantly smaller after high-fat diet than at baseline (ACh 0.1 microg/kg; 2.6+/-1.6 vs 1.0+/-0.5 nmol/L, P=0.03, ACh 0.4 microg/kg; 3.8+/-2.3 vs 1.8+/-1.1 nmol/L, P=0.04, respectively).
  
  
  CONCLUSIONS
  In high-fat-diet-induced obese dogs NO production was impaired in the early stage when the coronary flow response to ACh may be preserved.
authors.affiliation: Division of Cardiology, Kawasaki Medical School, Kurashiki, Japan.
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Kume
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okura
authors.forename: Yoji
authors.initials: Y
authors.lastname: Neishi
authors.forename: Ken
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hayashida
authors.forename: Nozomi
authors.initials: N
authors.lastname: Watanabe
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kanda
authors.forename: Seiichi
authors.initials: S
authors.lastname: Mochizuki
authors.forename: Masami
authors.initials: M
authors.lastname: Goto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D006328:Cardiac Catheterization / Q000295:instrumentation / Q000379:methods*; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000502:physiology*; D004041:Dietary Fats / Q000494:pharmacology; D004285:Dogs; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D007328:Insulin / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D014665:Vasodilator Agents; D009569:Nitric Oxide; D000109:Acetylcholine