Simpler noninstrumented batch and semicontinuous cultures provide mammalian cell kinetic data comparable to continuous and perfusion cultures.
Perfusion culture optimization in multiple noninstrumented small-scale flasks allows reduced expense and time associated with process development. These cultures normally use a different process mode because at small scales it is not practical to retain the cells for medium perfusion. In this work, the kinetics of growth, nutrient consumption, metabolite, and product formation were compared in spinner cultures operated in batch, semicontinuous, chemostat, and perfusion modes. Fed-batch was also included to provide an added comparison. Using logistic fitting for more reliable specific rate estimates in transient conditions, the growth phase of batch cultures predicted similar kinetics to fed-batch and continuous processes. For daily medium exchange rates up to 50%, the semicontinuous mode also predicted the perfusion process kinetics. Differences between the chemostat and semicontinuous culture results were only observed at higher exchange rates with the greatest daily culture perturbation. Overall, the batch or semicontinuous cultures were shown to readily provide results similar to the far more complex to operate chemostat or perfusion cultures.
Henry
Olivier
O
Dépt. de Génie Chimique, Ecole Polytechnique de Montréal, Campus de l'Université de Montréal, Montréal, QC, Canada H3C 3A7. olivier.henry@polymtl.ca
Kwok
Ezra
E
Piret
James M
JM
D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D006825:Hybridomas / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D010477:Perfusion; D051381:Rats