Quantitative determination of quinocetone and its metabolites in chicken plasma by liquid chromatography/tandem mass spectrometry.
A new method has been established for the simultaneous determination of the new veterinary drug quinocetone (3-methyl-2-cinnamoyl-N-1,4-dioxyquioxaline; QTN) and its 2 metabolites de-monoxy-quinocetone (3-methyl-2-cinnamoyl-N-1-monoxyquinoxaline; DMO-QTN) and de-dioxy-quinocetone (3-methyl-2-cinnamoyl-N-quinoxaline; DDI-QTN) in chicken plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The detection was performed using electrospray ionization in multiple reaction monitoring mode. The analysis of the linearity, accuracy, and precision of the method is described. The limits of detection and quantification of the LC/MS/MS method used for testing QTN, DMO-QTN, and DDI-QTN were 0.002 and 0.008 microg/mL; 0.002 and 0.008 microg/mL; and 0.003 and 0.010 microg/mL, respectively. The method was validated and can be used in future pharmacokinetic studies.
Li
Yong
Y
The Institute of Hygiology and Environmental Medical Research, Academy of Military Medical Sciences, Tianjin 300050, People's Republic of China.
Gao
ZhiXian
Z
Dong
FangTing
F
Cheng
YiYong
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002645:Chickens / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis; D007202:Indicators and Reagents; D000432:Methanol; D011786:Quality Control; D011810:Quinoxalines / Q000097:blood*; D012015:Reference Standards; D012996:Solutions; D012997:Solvents; D053719:Tandem Mass Spectrometry
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D011810:Quinoxalines; D012996:Solutions; D012997:Solvents; C502851:quinocetone; D000432:Methanol