title: Effect of gamma-irradiation on membrane fatty acids and peptidoglycan's muropeptides of Pantoea agglomerans, a plant pathogen.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of gamma-irradiation on the fatty acids (FA) and muropeptides content of two strains of an Enterobacteriacea: Pantoea agglomerans.
  
  
  RESULTS
  Pantoea agglomerans strains ATCC 49174 and RL1 isolated from irradiated carrots were used for this study. Radiation treatments (1 and 3.5 kGy) were performed to study the radiotolerance. Total lipids were obtained by multiple extractions using methanol/chloroform (2 : 1) and were quantified by GC. Muropeptides were purified by successive enzymatic digestions and analysed using a reverse phase C(18) column in high performance liquid chromatography. A significant (P <or= 0.05) modification of the bacterial wall was noticed for the membrane FA composition and the muropeptides.
  
  
  CONCLUSIONS
  Effects of irradiation on the bacterial membrane are noticeable and could play an important role on the cellular response and ability to survive this harsh environment.
  
  
  CONCLUSIONS
  To our knowledge, it is the first study to demonstrate the effects of ionizing irradiation on the modification of the FA and one of the few to confirm its effects on the muropeptides of the peptidoglycan.
authors.affiliation: Canadian Irradiation Centre and Research Laboratories in Sciences Applied to Food, INRS-Institut Armand-Frappier, Laval, QC, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Dussault
authors.forename: S
authors.initials: S
authors.lastname: Caillet
authors.forename: C
authors.initials: C
authors.lastname: Le Tien
authors.forename: M
authors.initials: M
authors.lastname: Lacroix
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000528:radiation effects*; D002851:Chromatography, High Pressure Liquid; D018552:Daucus carota / Q000382:microbiology; D005227:Fatty Acids / Q000032:analysis / Q000528:radiation effects; D005514:Food Irradiation / Q000379:methods*; D005720:Gamma Rays; D020636:Pantoea / Q000528:radiation effects*; D010457:Peptidoglycan / Q000032:analysis / Q000528:radiation effects*
chemical_list: D005227:Fatty Acids; D010457:Peptidoglycan