Myosin-IIA and ICAM-1 regulate the interchange between two distinct modes of T cell migration.
How T cells achieve rapid chemotactic motility under certain circumstances and efficient cell surface surveillance in others is not fully understood. We show that T lymphocytes are motile in two distinct modes: a fast "amoeboid-like" mode, which uses sequential discontinuous contacts to the substrate; and a slower mode using a single continuously translating adhesion, similar to mesenchymal motility. Myosin-IIA is necessary for fast amoeboid motility, and our data suggests that this occurs via cyclical rear-mediated compressions that eliminate existing adhesions while licensing subsequent ones at the front of the cell. Regulation of Myosin-IIA function in T cells is thus a key mechanism to regulate surface contact area and crawling velocity within different environments. This can provide T lymphocytes with motile and adhesive properties that are uniquely suited toward alternative requirements for immune surveillance and response.
Jacobelli
Jordan
J
Department of Pathology, University of California San Francisco, San Francisco CA 94143, USA.
Bennett
F Chris
FC
Pandurangi
Priya
P
Tooley
Aaron J
AJ
Krummel
Matthew F
MF
D000818:Animals; D002448:Cell Adhesion / Q000276:immunology; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology*; D015151:Immunoblotting; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D024507:Nonmuscle Myosin Type IIA / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
D018799:Intercellular Adhesion Molecule-1; D024507:Nonmuscle Myosin Type IIA