Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney.
OBJECTIVE
To compare the effect of short-term calorie restriction (CR) on aging with that of already known long-term CR, the anti-inflammatory efficacy of 10-day CR was explored in aged rat kidney.


METHODS
Two different age groups, 6 months (young) and 24 months (old) were used. In the old group, one sub-group was control, fed ad libitum (AL) and the other was fed CR for 10 days with 40 % of the food intake of the AL subgroup (n = 5).


METHODS
Reactive species (RS), lipid peroxides and COX-2 activity were measured. The activities of proinflammatory transcription factors NF-kB and AP-1 were measured by electro-mobility shift assay (EMSA). Upstream signaling cascades of NF-kB and AP-1 as well as proinflammatory gene expression were detected by Western blot.


RESULTS
10-day CR suppressed RS, lipid peroxides, and COX-2 activity in aged rat kidney. CR also inhibited upstream signaling cascades and DNA binding activity of NF-kB and AP-1, and thioredoxin/Ref-1 pathway. CR blocked expression of NF-kB-and AP-1-responsive gene COX-2, iNOS, VCAM-1 and ICAM-1.


CONCLUSIONS
We report for the first time that 10-day CR can attenuate the altered signaling transduction for inflammatory processes which is mediated through RS-induced NF-kB and AP-1 in aged kidney.
Jung
K J
KJ
Department of Pharmacy, Pusan National University, Busan, Korea.
Lee
E K
EK
Kim
J Y
JY
Zou
Y
Y
Sung
B
B
Heo
H S
HS
Kim
M K
MK
Lee
J
J
Kim
N D
ND
Yu
B Pal
BP
Chung
H Y
HY
D000375:Aging / Q000502:physiology*; D000818:Animals; D031204:Caloric Restriction; D004032:Diet; D007249:Inflammation / Q000276:immunology*; D007668:Kidney / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D052002:NF-kappa B p50 Subunit; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D011451:Prostaglandin-Endoperoxide Synthases; D020928:Mitogen-Activated Protein Kinases