title: Islet encapsulation with living cells for improvement of biocompatibility.
abstract: Bioartificial pancreas, microencapsulation of islets of Langerhans (islets) within devices has been studied as a safe and simple technique for islet transplantation without the need for immuno-suppressive therapy. Various types of bioartificial pancreas have been proposed and developed such as microcapsule, macrocapsule and diffusion chamber types. However, these materials comprising a bioartificial pancreas are not completely inert and may induce foreign body and inflammatory reactions. The residual materials would be a problem in human body. Here we propose an alternative method for microencapsulation of islets with a layer of living cells. We immobilized HEK293 cells (human endoderm kidney cell line) to the islet surface using amphiphilic poly(ethylene glycol)-conjugated phospholid derivative and biotin/streptavidin reaction and encapsulated islets with a cell layer by culture. No necrosis of islet cells at the center was seen after microencapsulation with a layer of living cells. Insulin secretion ability by glucose stimulation was well maintained on these cell-encapsulated islets.
authors.affiliation: Department of Nano-Medicine Merger Education Unit, Graduate School of Engineering, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Teramura
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D002470:Cell Survival; D018914:Cells, Immobilized; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D007328:Insulin; D008055:Lipids; D011092:Polyethylene Glycols; D005947:Glucose