title: [Effects of mechanical stress on the formation and expression of Cbfalpha1 in vitro].
abstract: OBJECTIVE
  To evaluate the effects of mechanical stress on the formation and expression of core binding factor alpha1 (Cbfalpha1) in MG-63 cells cultured on titanium in vitro.
  
  
  METHODS
  MG-63 cells cultured on the titanium were subjected to a centrifugal force (2.205 N) 15 min per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfalpha1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force (P<0.05). Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfalpha1 and the relative mRNA level of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significant (P<0.05).
  
  
  CONCLUSIONS
  Mechanical stress are beneficial to the formation and expression of Cbfalpha1.
authors.affiliation: Dept. of Stomatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors.forename: Xue-jin
authors.initials: XJ
authors.lastname: Wang
authors.forename: Xiao-ning
authors.initials: XN
authors.lastname: Hao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Xu
mesh_terms: D050659:Core Binding Factor Alpha 1 Subunit; D066298:In Vitro Techniques; D012333:RNA, Messenger; D013314:Stress, Mechanical
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger