title: Methylation profiles in breast cancer.
abstract: In this study, we sought to assess the aberrant methylation of multiple tumor-suppressor genes in a single reaction by using methylation-specific multiplex ligation-dependent probe amplification. Breast tumors and corresponding normal tissues of 77 patients were analyzed. In this study, 17 of 24 genes displayed promoter methylation in one or more of the tumor samples. The most frequently methylated genes were RASSF1 and GSTP1, followed by DAPK1 and CDKN2B. Our data indicate that the methylation of specific genes is a frequent event in breast cancer and show that MS-MLPA is a powerful tool to analyze epigenetic alterations for diagnostic, as well as therapeutic, purposes.
authors.affiliation: Department of Medical Biology, Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey.
authors.forename: Nur
authors.initials: N
authors.lastname: Buyru
authors.forename: Julide
authors.initials: J
authors.lastname: Altinisik
authors.forename: Filiz
authors.initials: F
authors.lastname: Ozdemir
authors.forename: Semra
authors.initials: S
authors.lastname: Demokan
authors.forename: Nejat
authors.initials: N
authors.lastname: Dalay
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics; D019175:DNA Methylation; D064550:Death-Associated Protein Kinases; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D011401:Promoter Regions, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; C493966:CDKN2B protein, human; D050762:Cyclin-Dependent Kinase Inhibitor p15; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; C579096:DAPK1 protein, human; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases