Production of fusion mussel adhesive fp-353 in Escherichia coli.
Mussel adhesive proteins (MAPs) have a potential as environmentally friendly adhesives for use under aqueous conditions. MAPs maybe of particular value in medical applications. We previously reported the functional expression of recombinant foot protein type 5 (fp-5) and foot protein type 3A (fp-3A), both of which have significant adhesion abilities, in Escherichia coli. However, these proteins were produced at low levels because of post-induction cell growth inhibition, and the proteins showed poor post-purification solubility. Here, we design and produce a new type of recombinant MAP, fp-353, that is a fusion protein with fp-3A at each terminus of fp-5. Because fp-353 formed inclusion bodies, host cell growth inhibition did not occur. In addition, the solubility of MAP fp-353 after purification was significantly enhanced, permitting the preparation of a viscous concentrated glue solution for large-scale adhesion strength measurements. Together with large-scale cowhide adhesion measurements and cell-adhesion analyses, we successfully demonstrated that fusion mussel protein fp-353 has potential as a practical alternative bioadhesive.
Gim
Youngsoo
Y
Dept. of Chemical Engineering, National Research Laboratory of Molecular Biotechnology, Pohang University of Science and Technology, Pohang, Korea.
Hwang
Dong Soo
DS
Lim
Seonghye
S
Song
Young Hoon
YH
Cha
Hyung Joon
HJ
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D014442:Monophenol Monooxygenase / Q000378:metabolism; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
D011506:Proteins; D011993:Recombinant Fusion Proteins; C037403:adhesive protein, mussel; D014442:Monophenol Monooxygenase