title: Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences.
abstract: We have developed a new red fluorogenic compound derived from naphthorhodamine for a reduction-triggered fluorescence probe to sense oligonucleotides. The fluorogenic reaction between naphthorhodamine azide derivatives and reducing reagents such as triphenylphosphine (TPP) on the DNA target does not use any enzyme or reagent, and fluoresces at 650 nm. The probes were used for dual color detection of a single nucleotide difference on the leukemia-related bcr/abl gene.
authors.affiliation: Nano Medical Engineering Laboratory, Advance Science Institute, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Miwako
authors.initials: M
authors.lastname: Uda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koshino
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D001483:Base Sequence; D003116:Color; D004252:DNA Mutational Analysis / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D016313:Genes, abl / Q000235:genetics; D006801:Humans; D015336:Molecular Probe Techniques; D009841:Oligonucleotides / Q000032:analysis / Q000235:genetics*; D010084:Oxidation-Reduction; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012235:Rhodamines
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012235:Rhodamines