Evaluation of chaperone-like activity of alginate: microcapsule and water-soluble forms.
To evaluate the chaperone-like activity of alginate stabilization and refolding of alkaline phosphatase (ALP) was investigated in the presence of alginate through two different approaches, the soluble form and microcapsule assisted methods. It was found that in the presence of microcapsules, ALP can be stabilized to a higher degree compared with the water-soluble form, whereas the denatured ALP is refolded with a higher yield through latter method. Lower refolding yields of alginate beads compared with its soluble form may be the result of lower refolding rate of ALP upon elution of the bound enzyme by dispersing the precipitate in NaCl which left the unfolded protein in an unsuitable environment, providing enough time for protein aggregation and leading finally to lower recovered activity compared with application of soluble form of alginate. In addition in the case of alginate capsules, the choice of suitable divalent ion is essential for stability and assistance in refolding.
Rezaii
Neguine
N
Neuroscience Research Center, Shahid Beheshti University (M.C.), Tehran, Iran.
Khodagholi
Fariba
F
D000464:Alginates / Q000737:chemistry* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry; D008863:Microspheres; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D012965:Sodium Chloride / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry
D000464:Alginates; D006603:Hexuronic Acids; D018832:Molecular Chaperones; D012965:Sodium Chloride; D003300:Copper; D020723:Glucuronic Acid; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc; D002118:Calcium