title: Phenylalanine containing hydrophobic nanospheres for antibody purification.
abstract: In this study, novel hydrophobic nanospheres with an average size of 158 nm utilizing N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) as a hydrophobic monomer were produced by surfactant free emulsion polymerization of 2-hydroxyethyl methacrylate (HEMA) and MAPA conducted in an aqueous dispersion medium. MAPA was synthesized using methacryloyl chloride and L-phenylalanine methyl ester. Specific surface area of the nonporous nanospheres was found to be 1874 m2/g. Poly(HEMA-MAPA) nanospheres were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Average particle size, size distribution, and surface charge measurements were also performed. Elemental analysis of MAPA for nitrogen was estimated as 0.42 mmol/g polymer. Then, poly(HEMA-MAPA) nanospheres were used in the adsorption of immunoglobulin G (IgG) in batch system. Higher adsorption values (780 mg/g) were obtained when the poly (HEMA-MAPA) nanospheres were used from both aqueous solutions and human plasma. The adsorption phenomena appeared to follow a typical Langmuir isotherm. It was observed that IgG could be repeatedly adsorbed and desorbed without significant loss in adsorption amount. These findings show considerable promise for this material as a hydrophobic support in industrial processes.
authors.affiliation: Dept. of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Türkmen
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
authors.forename: Nevra
authors.initials: N
authors.lastname: Oztürk
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.forename: Assem
authors.initials: A
authors.lastname: Elkak
mesh_terms: D000327:Adsorption; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D007074:Immunoglobulin G; D008689:Methacrylates; D011108:Polymers; D012492:Salts; D012996:Solutions; D014867:Water; D010649:Phenylalanine; C005044:hydroxyethyl methacrylate