High-affinity labeling and tracking of individual histidine-tagged proteins in live cells using Ni2+ tris-nitrilotriacetic acid quantum dot conjugates.
Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for QD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates tris-nitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using QD-trisNTA, we demonstrate two-color QD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein-protein interactions at the single molecule level.
Roullier
Victor
V
Université de Rennes, Sciences Chimiques de Rennes, CNRS UMR 6226, Campus de Beaulieu, 35042 Rennes Cedex, France.
Clarke
Samuel
S
You
Changjiang
C
Pinaud
Fabien
F
Gouzer
G Géraldine
GG
Schaible
Dirk
D
Marchi-Artzner
Valérie
V
Piehler
Jacob
J
Dahan
Maxime
M
D001672:Biocompatible Materials / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry; D002448:Cell Adhesion; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D011092:Polyethylene Glycols; D045663:Quantum Dots; D017471:Receptors, Interferon / Q000378:metabolism; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
D001672:Biocompatible Materials; D019187:Cadmium Compounds; C485182:Cyan Fluorescent Protein; D017471:Receptors, Interferon; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D006639:Histidine; D009532:Nickel; C058667:cadmium selenide; D009571:Nitrilotriacetic Acid; C031238:zinc sulfide