title: Vibrio gallaecicus sp. nov. isolated from cultured clams in north-western Spain.
abstract: A group of three motile facultative anaerobic marine bacteria were isolated from cultured Manila clams (Ruditapes philippinarum) in Galicia, north-western Spain. The strains were characterized phenotypically and genotypically. Phylogenetic analysis of the 16S rRNA gene and four housekeeping genes, RNA polymerase alpha-chain (rpoA), RecA protein (recA), the alpha-subunit of bacterial ATP synthase (atpA) and the uridine monophosphate (UMP) kinase (pyrH), indicated that these strains were closely related to the Vibrio splendidus clade. The amplified fragment length polymorphism (AFLP) fingerprints, DNA-DNA hybridizations and phylogenies of the housekeeping and 16S rRNA gene sequences showed that the three strains represented a different species from all currently described vibrios. The new species could be differentiated from its nearest neighbours on the basis of several phenotypic features. The three strains are therefore a novel species within the genus Vibrio, for which the name Vibrio gallaecicus is proposed, with the type strain being VB 8.9T(=CECT 7244T=LMG 24045T).
authors.affiliation: Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Roxana
authors.initials: R
authors.lastname: Beaz-Hidalgo
authors.forename: Alejandra
authors.initials: A
authors.lastname: Doce
authors.forename: Javier
authors.initials: J
authors.lastname: Pascual
authors.forename: Alicia E
authors.initials: AE
authors.lastname: Toranzo
authors.forename: Jesús L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000818:Animals; D017756:Aquaculture; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D015373:Bacterial Typing Techniques; D049872:Bivalvia / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013030:Spain; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S