Identification of novel peptides specifically binding to endometriosis by screening phage-displaying peptide libraries.
OBJECTIVE
To search for novel peptides and common binding motif that specifically bind to endometriosis.


METHODS
Prospective study.


METHODS
Department of Biological Science and Technology in national university.


METHODS
Specimens were divided into [1] ectopic endometrium (n = 10); [2] eutopic endometrium (n = 10).


METHODS
Peptides specifically binding to endometriosis are screened from a phage-displaying peptide library (Ph.D.-12) by using whole-cell screening technique after an adsorption elution amplification procedure.


METHODS
Combinatorial peptide libraries were used to identify small molecules that bind with high affinity to receptor molecules and mimic the interaction with natural ligands. Few pans of positive phage clones with significantly positive signals were identified by ELISA and analyzed by DNA sequencing.


RESULTS
During the biopanning processes, the recovered phage number (10(6) pfu/mL) in parts 1, 2, 3, 4, and 5 of the study were 9, 33, 82, 142, and 169. Nine phages consistently had residue Arg, whereas six clones had a consensus motif of Arg-X-Arg-X-X-X-X-Arg. The biotin-labeled peptide bound to endometriosis cells in a dose-dependent manner, yet the control peptide revealed lesser binding activity.


CONCLUSIONS
The novel motif is associated with higher affinity of endometriosis, which might be useful in endometriosis targeting and as potential antiendometriosis therapies. We provide one potential approach for novel therapies toward endometriosis.
Chang
Chi-Chen
CC
Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan.
Hsieh
Yao-Yuan
YY
Wang
Yu-Kuo
YK
Hsu
Kung-Hao
KH
Tsai
Horng-Der
HD
Tsai
Fuu-Jen
FJ
Lin
Chih-Sheng
CS
D000595:Amino Acid Sequence; D016384:Consensus Sequence; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D011446:Prospective Studies; D017422:Sequence Analysis, DNA
D019151:Peptide Library; D010455:Peptides