title: Cell wall component and mycotoxin moieties involved in the binding of fumonisin B1 and B2 by lactic acid bacteria.
abstract: OBJECTIVE
  The ability of lactic acid bacteria (LAB) to bind fumonisins B1 and B2 (FB1, FB2) in fermented foods and feeds and in the gastrointestinal tract could contribute to decrease their bioavailability and toxic effects on farm animals and humans. The aim of this work was to identify the bacterial cell wall component(s) and the functional group(s) of FB involved in the LAB-FB interaction.
  
  
  RESULTS
  The effect of physicochemical, enzymatic and genetic treatments of bacteria and the removal/inactivation of the functional groups of FB on toxin binding were evaluated. Treatments affecting the bacterial wall polysaccharides, lipids and proteins increased binding, while those degrading peptidoglycan (PG) partially decreased it. In addition, purified PG from Gram-positive bacteria bound FB in a manner analogue to that of intact LAB. For FB, tricarballylic acid (TCA) chains play a significant role in binding as hydrolysed FB had less affinity for LAB.
  
  
  CONCLUSIONS
  Peptidoglycan and TCA are important components of LAB and FB, respectively, involved in the binding interaction.
  
  
  CONCLUSIONS
  Lactic acid bacteria binding efficiency seems related to the peptide moiety structure of the PG. This information can be used to select probiotics with increased FB binding efficiency.
authors.affiliation: INRA, UR1213 Herbivores, Saint-Genès Champanelle, France.
authors.forename: V
authors.initials: V
authors.lastname: Niderkorn
authors.forename: D P
authors.initials: DP
authors.lastname: Morgavi
authors.forename: B
authors.initials: B
authors.lastname: Aboab
authors.forename: M
authors.initials: M
authors.lastname: Lemaire
authors.forename: H
authors.initials: H
authors.lastname: Boudra
mesh_terms: D001665:Binding Sites; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D037341:Fumonisins / Q000737:chemistry / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D009183:Mycotoxins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C056933:fumonisin B1