Biofunctionalization of dispense-plotted hydroxyapatite scaffolds with peptides: quantification and cellular response.
Hydroxyapatite (HA) ceramic is a widely used synthetic bone substitute material for the regeneration of bone defects. We manufactured HA scaffolds with adjustable pore sizes and pore geometry by dispense-plotting. In addition, we attached peptides covalently onto the HA surface and are able to simultaneously quantify the amount of covalently attached and adsorbed peptide down to the picomolar range with a novel fluorescence-based detection method. In cell culture assays with stromal bone marrow cells, we observed a positive effect of biofunctionalization on cell differentiation after 21 days of culture when comparing the scaffold functionalized with the RGD motif containing adhesion peptide to an unmodified scaffold.
Detsch
Rainer
R
BioCer Entwicklungs-GmbH, Ludwig-Thoma-Str. 36 c, 95447 Bayreuth, Germany.
Dieser
Irina
I
Deisinger
Ulrike
U
Uhl
Franziska
F
Hamisch
Sabine
S
Ziegler
Günter
G
Lipps
Georg
G
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D002477:Cells / Q000187:drug effects / Q000648:ultrastructure; D017886:Durapatite / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds / Q000737:chemistry*; D014158:Transcription, Genetic
D001672:Biocompatible Materials; D018786:Bone Substitutes; D010455:Peptides; D017886:Durapatite; D007770:L-Lactate Dehydrogenase