title: Molecular characterization and in situ quantification of anoxic arsenite-oxidizing denitrifying enrichment cultures.
abstract: To explore the bacteria involved in the oxidation of arsenite (As(III)) under denitrifying conditions, three enrichment cultures (ECs) and one mixed culture (MC) were characterized that originated from anaerobic environmental samples. The oxidation of As(III) (0.5 mM) was dependent on NO(3) (-) addition and N(2) formation was dependent on As(III) addition. The ratio of N(2)-N formed to As(III) fed approximated the expected stoichiometry of 2.5. A 16S rRNA gene clone library analysis revealed three predominant phylotypes. The first, related to the genus Azoarcus from the division Betaproteobacteria, was found in the three ECs. The other two predominant phylotypes were closely related to the genera Acidovorax and Diaphorobacter within the Comamonadaceae family of Betaproteobacteria, and one of these was present in all of the cultures examined. FISH confirmed that Azoarcus accounted for a large fraction of bacteria present in the ECs. The Azoarcus clones had 96% sequence homology with Azoarcus sp. strain DAO1, an isolate previously reported to oxidize As(III) with nitrate. FISH analysis also confirmed that Comamonadaceae were present in all cultures. Pure cultures of Azoarcus and Diaphorobacter were isolated and shown to be responsible for nitrate-dependent As(III) oxidation. These results, taken as a whole, suggest that bacteria within the genus Azoarcus and the family Comamonadaceae are involved in the observed anoxic oxidation of As(III).
authors.affiliation: Department of Chemical and Environmental Engineering, University of Arizona, Tucson, AZ, USA.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Sun
authors.forename: Reyes
authors.initials: R
authors.lastname: Sierra-Alvarez
authors.forename: Nuria
authors.initials: N
authors.lastname: Fernandez
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Sanz
authors.forename: Ricardo
authors.initials: R
authors.lastname: Amils
authors.forename: Antje
authors.initials: A
authors.lastname: Legatzki
authors.forename: Raina M
authors.initials: RM
authors.lastname: Maier
authors.forename: Jim A
authors.initials: JA
authors.lastname: Field
mesh_terms: D018053:Arsenites / Q000378:metabolism*; D020627:Azoarcus / Q000235:genetics* / Q000378:metabolism; D042621:Comamonadaceae / Q000235:genetics*; D003470:Culture Media; D015723:Gene Library; D020459:Genes, rRNA; D006885:Hydroxybutyrates / Q000032:analysis; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018053:Arsenites; D003470:Culture Media; D006885:Hydroxybutyrates; D009566:Nitrates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C015001:arsenite