Intracellular adaptation of Brucella abortus.
Macrophages were infected with virulent Brucella abortus strain 2308 or attenuated strain 19. Intracellular bacteria were recovered at different times after infection and their proteomes compared. The virulent strain initially reduced most biosynthesis and altered its respiration; adaptations reversed later in infection. The attenuated strain was unable to match the magnitude of the virulent strain's adjustments. The results provide insight into mechanisms utilized by Brucella to establish intracellular infections.
Lamontagne
Julie
J
Caprion Proteomics, Inc., 7150 Alexander-Fleming, Montreal, Quebec, Canada, H4S 2C8.
Forest
Anik
A
Marazzo
Elena
E
Denis
François
F
Butler
Heather
H
Michaud
Jean-François
JF
Boucher
Lyne
L
Pedro
Ida
I
Villeneuve
Annie
A
Sitnikov
Dmitri
D
Trudel
Karine
K
Nassif
Najib
N
Boudjelti
Djamila
D
Tomaki
Fadi
F
Chaves-Olarte
Esteban
E
Guzmán-Verri
Caterina
C
Brunet
Sylvain
S
Côté-Martin
Alexandra
A
Hunter
Joanna
J
Moreno
Edgardo
E
Paramithiotis
Eustache
E
D001426:Bacterial Proteins / Q000378:metabolism*; D002003:Brucella abortus / Q000378:metabolism*; D002460:Cell Line; D002853:Chromatography, Liquid; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D020543:Proteome