title: Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.
abstract: Alanine racemase (DadX(OF4)), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P2(1)2(1)2(1), with two protein molecules in the asymmetric unit.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ju
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Shujing
authors.initials: S
authors.lastname: Xu
authors.forename: Kouhei
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Benedik
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Xue
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000411:Alanine Racemase / Q000737:chemistry* / Q000302:isolation & purification; D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration
chemical_list: D000411:Alanine Racemase