Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri.
Maltose-binding protein is the periplasmic component of the ABC transporter responsible for the uptake of maltose/maltodextrins. The Xanthomonas axonopodis pv. citri maltose-binding protein MalE has been crystallized at 293 K using the hanging-drop vapour-diffusion method. The crystal belonged to the primitive hexagonal space group P6(1)22, with unit-cell parameters a = 123.59, b = 123.59, c = 304.20 A, and contained two molecules in the asymetric unit. It diffracted to 2.24 A resolution.
Souza
C S
CS
Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, SP, Brazil.
Ferreira
L C S
LC
Thomas
L
L
Barbosa
J A R G
JA
Balan
A
A
D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D017124:Conserved Sequence; D003460:Crystallization; D003625:Data Collection / Q000379:methods; D008320:Maltose / Q000737:chemistry / Q000378:metabolism; D058619:Maltose-Binding Proteins; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D053532:Xanthomonas axonopodis / Q000737:chemistry* / Q000472:pathogenicity
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011134:Polysaccharides; D008320:Maltose; C008315:maltodextrin