title: Long-term marginal zinc supply is not detrimental to the skeleton of aged female rats.
abstract: In this experiment, we investigated the long-term effects of a marginal zinc (Zn) supply on bone metabolism in aged rats. Nine-mo-old female Fischer-344 rats were divided into 8 weight-matched groups of 8 rats each. All rats were adapted for 1 mo to restrictive feeding (7.5 g/d) of a purified diet containing 8 g/kg sodium phytate and 64 mg/kg Zn. Control rats were pair-fed throughout the experiment. During the 1-mo depletion phase, controls received the Zn-replete diet with 64 mg/kg Zn, whereas Zn-deficient rats were fed the same diet with 2.2 mg/kg Zn. The depletion phase was followed by a 3-mo marginal phase in which the rats fed the diet with 2.2 mg/kg Zn received an additional daily Zn supplement of 75 microg Zn/rat by gavage. In the following 2-mo repletion phase, a marginal group was switched to the Zn-replete diet, while the other groups were maintained on marginal Zn supply or on the Zn-replete diet. Zn depletion and marginal Zn reduced serum and bone Zn and serum alkaline phosphatase activity. Zn repletion normalized serum Zn. However, apart from subtle changes in bone mineralization density distribution, Zn deficiency was not associated with detrimental effects on bone mineral density, turnover, architecture, or biomechanics relative to control rats at any time point. Our data suggest that Zn does not play an essential role in bone metabolism in aged rats and cast doubt on the hypthosis that Zn deficiency is a risk factor for osteoporosis.
authors.affiliation: Institute of Physiology, Physiological Chemistry and Animal Nutrition, Ludwig Maximilians University, 80539 Munich, Germany. reinhold.erben@vu-wien.ac.at
authors.forename: Reinhold G
authors.initials: RG
authors.lastname: Erben
authors.forename: Katja
authors.initials: K
authors.lastname: Lausmann
authors.forename: Paul
authors.initials: P
authors.lastname: Roschger
authors.forename: Christiane
authors.initials: C
authors.lastname: Schüler
authors.forename: Monika
authors.initials: M
authors.lastname: Skalicky
authors.forename: Klaus
authors.initials: K
authors.lastname: Klaushofer
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Windisch
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D051381:Rats; D011916:Rats, Inbred F344; D016474:Weight-Bearing; D015032:Zinc / Q000494:pharmacology*
chemical_list: D015032:Zinc