In vitro distribution of gold in serum proteins after incubation of sodium aurothiomalate and auranofin with human blood and its pharmacological significance.
This study presents a comparative drug-protein, in vitro, binding profile of sodium aurothiomalate and auranofin. It was found that about 40% of total protein-bound gold is attached to albumin after incubation of aurothiomalate with whole blood for 24 h and about 29% of it was with alpha(1)-globulin and the least amount was found with gamma-globulin (6.1%). On the other hand, approximately 84% of the protein-bound auranofin gold attached to globulins of which 51% was found with beta-globulin band. It was almost equally distributed among albumin, alpha(2)-globulin and gamma-globulin, and showed least affinity for alpha(1)-globulin. The gold analyses were performed by standardless instrumental neutron activation method duly validated by use of an established atomic absorption method. The results of this study explain to some extent the difference in, in vivo, pharmacokinetics and pharmacodynamics of the two drugs.
Iqbal
Mohammad S
MS
Department of Chemistry, GC University, Lahore 54000, Pakistan. saeediq50@hotmail.com
Taqi
Syed G
SG
Arif
Muhammad
M
Wasim
Muhammad
M
Sher
Muhammad
M
D001310:Auranofin / Q000378:metabolism*; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D006046:Gold / Q000032:analysis / Q000378:metabolism*; D006052:Gold Sodium Thiomalate / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009501:Neutron Activation Analysis; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D012712:Serum Globulins / Q000737:chemistry / Q000378:metabolism
D001798:Blood Proteins; D012709:Serum Albumin; D012712:Serum Globulins; D006052:Gold Sodium Thiomalate; D001310:Auranofin; D006046:Gold