Cellular internalization and in vivo tracking of thermosensitive luminescent micelles based on luminescent lanthanide chelate.
An amphiphilic tris(dibenzoylmethanato)europium(III) (Eu(DBM)(3)) coordinated P(MMA-co-EIPPMMA)-co-P(NIPAAm-co-NDAPM) copolymer was synthesized, which exhibited good biocompatibility and emitted strong red luminescence (MMA, methyl methacrylate; EIPPMMA, 4-(1-ethyl-1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenyl methacrylate; NIPAAm, N-isopropylacrylamide; NDAPM, (N-(3-dimethylamino)propyl)methacrylamide). The copolymer could self-assemble into micelles of size around 260 nm, and the micelles were thermosensitive at around body temperature. The drug-loaded micelles showed thermosensitive controlled drug release, and the paclitaxel loaded micelles were capable of being internalized into the tumor cells (A549) and exhibited obvious inhibition to the growth of A549 cells. Importantly, in vivo study showed the self-assembled micelles of Eu(DBM)(3) coordinated P(MMA-co-EIPPMMA)-co-P(NIPAAm-co-NDAPM) copolymer uptaken by the larvae of zebrafish could be easily tracked and be eliminated from the body within several days.
Li
Yong-Yong
YY
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
Cheng
Han
H
Zhang
Zhi-Guo
ZG
Wang
Chang
C
Zhu
Jing-Ling
JL
Liang
Yong
Y
Zhang
Ke-Li
KL
Cheng
Si-Xue
SX
Zhang
Xian-Zheng
XZ
Zhuo
Ren-Xi
RX
D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry*; D004337:Drug Carriers / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008823:Micelles; D009942:Organometallic Compounds / Q000737:chemistry; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013500:Surface Tension; D013696:Temperature; D015027:Zebrafish / Q000196:embryology
D002614:Chelating Agents; D004337:Drug Carriers; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D008823:Micelles; D009942:Organometallic Compounds; D011108:Polymers; D017239:Paclitaxel