Assessment of anti-oxidant activity of plant extracts using microbial test systems.
OBJECTIVE
To evaluate the anti-oxidant properties of extracts from 20 medicinal herbs growing in western Siberia using microbial test systems and different in vitro methods.


RESULTS
In vivo anti-oxidant activity of extracts was evaluated for their capacity to protect bacteria, Escherichia coli, against bacteriostatic and bactericidal effects of H(2)O(2) and menadione, and action on anti-oxidant gene expression. In vitro anti-oxidant activity has been examined by a number of methods including: the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH(*))-scavenging assay, chelating activity and capacity to protect plasmid DNA against oxidative damage. In addition, total polyphenol content was determined. The extracts of Fragaria vesca, Rosa majalis, Pentaphylloides fruticosa, Alchemilla vulgaris and Pulmonaria mollis possessed the highest levels of anti-oxidant activity in vivo and in vitro. The protective properties were more closely related to the DPPH(*) radical-scavenging activity, tannin content and action on anti-oxidant gene expression than to other parameters.


CONCLUSIONS
The extracts of medicinal plants may have anti-oxidant effects on bacteria simultaneously through several different pathways, including direct inhibition of reactive oxygen species, iron chelation and anti-oxidant genes induction.


CONCLUSIONS
Using microbial test systems, we revealed herbs that may be used as potential sources of natural anti-oxidants.
Oktyabrsky
O
O
Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, Perm, Russia. oktyabr@iegm.ru
Vysochina
G
G
Muzyka
N
N
Samoilova
Z
Z
Kukushkina
T
T
Smirnova
G
G
D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000032:analysis; D002374:Catalase / Q000032:analysis*; D053960:DNA Breaks; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005419:Flavonoids / Q000032:analysis; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D024483:Vitamin K 3 / Q000494:pharmacology; D001616:beta-Galactosidase / Q000032:analysis*
D000975:Antioxidants; D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D005419:Flavonoids; D010851:Picrates; D010936:Plant Extracts; D024483:Vitamin K 3; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D001616:beta-Galactosidase