Langmuir-Blodgett film based on MEH-PPV for cholesterol biosensor.
Cholesterol oxidase (ChOx) has been immobilized onto conducting poly[2-methoxy,5-(2'-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV)/stearic acid (SA) Langmuir-Blodgett film transferred onto octadecanethiol (ODT) modified gold plate. The ChOx/MEH-PPV/SA LB film bioelectrode exhibits has been characterized by FT-IR, contact angle, and atomic force microscopy. The response of the ChOx/MEH-PPV/SA LB film bioelectrode carried out using differential pulse voltammetry (DPV) studies reveal linearity from 1.29 to 12.91 mM of cholesterol concentration and response time as 30 s. This ChOx/MEH-PPV/SA bioelectrode exhibits values of correlation coefficient as 0.9939, standard deviation as 0.0029 microA and limit of detection as 1.66 mM. UV-visible spectrophotometer studies reveal that 5.2x10(-3) U of ChOx are actively working per cm(2) area of ChOx/MEH-PPV/SA LB film bioelectrode and this bioelectrode is thermally stable upto 55 degrees C with reusability of about 60 times.
Matharu
Zimple
Z
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India.
Arya
Sunil K
SK
Singh
S P
SP
Gupta
Vinay
V
Malhotra
B D
BD
D015374:Biosensing Techniques / Q000379:methods*; D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase / Q000378:metabolism*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D008567:Membranes, Artificial; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013229:Stearic Acids / Q000737:chemistry; D013499:Surface Properties; D014753:Vinyl Compounds / Q000737:chemistry*
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011108:Polymers; D013229:Stearic Acids; D014753:Vinyl Compounds; C520682:poly(2-methoxy-5-(2'-ethylhexyloxy)-p-phenylenevinylene); C031183:stearic acid; D006046:Gold; D002784:Cholesterol; D002789:Cholesterol Oxidase