Inhibition of selectin binding by colloidal gold with functionalized shells.
Gold colloids with terminally functionalized sulfated thiol shells were found to bind to P- and L-selectins with IC(50) values in the picomolar range; branched acyclic epitopes show the highest affinity, whereas a sulfated carbohydrate mimetic provides the best selectivity.
Dernedde
Jens
J
Charité-Universitätsmedizin Berlin, CBF, Zentralinstitut für Laboratoriumsmedizin und Pathobiochemie, Hindenburgdamm 30, 12203 Berlin, Germany. jens.dernedde@charite.de
Enders
Sven
S
Reissig
Hans-Ulrich
HU
Roskamp
Meike
M
Schlecht
Sabine
S
Yekta
Shahla
S
D000818:Animals; D001665:Binding Sites; D003260:Contact Inhibition; D004587:Electrophoresis, Agar Gel; D017979:Gold Colloid / Q000737:chemistry* / Q000378:metabolism; D020128:Inhibitory Concentration 50; D019041:L-Selectin / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008024:Ligands; D019007:P-Selectin / Q000378:metabolism*
D017979:Gold Colloid; D008024:Ligands; D019007:P-Selectin; D019041:L-Selectin