FTICR-MS and LC-UV/MS-SPE-NMR applications for the rapid dereplication of a crude extract from the sponge Ianthella flabelliformis.
Dereplication of a methanolic extract of the marine sponge Ianthella flabelliformis using FTICR-MS accurate mass determination and MS(n) techniques enabled rapid and unambiguous detection of a new compound among a plethora of known compounds. Isolation of this compound and the known 19-deoxy analogue using the hyphenated technique LC-UV/MS-SPE-NMR was undertaken, and the structures were confirmed, from a single chromatographic run, as 19-hydroxyaraplysillin-I N(20)-sulfamate (1) and araplysillin-I N(20)-sulfamate (2).
Motti
Cherie A
CA
Australian Institute of Marine Science, Australia. C.Motti@aims.gov.au
Freckelton
Marnie L
ML
Tapiolas
Dianne M
DM
Willis
Richard H
RH
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006842:Hydrocarbons, Brominated / Q000737:chemistry / Q000302:isolation & purification*; D007555:Isoxazoles / Q000737:chemistry / Q000302:isolation & purification*; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011161:Porifera / Q000737:chemistry*
C550403:19-hydroxyaraplysillin-I N(20)-sulfamate; D006842:Hydrocarbons, Brominated; D007555:Isoxazoles; C550402:araplysillin-I N(20)-sulfamate