[Study on repair of subacute spinal cord injury by transplantation of olfactory ensheathing cells combined with chondroitinase ABC in adult rats].
OBJECTIVE
To investigate the synergetic effect and possibility of repairing spinal cord injury (SCI) by transplantation of olfactory ensheathing cells (OECs) and chondroitinase ABC (ChABC) in adult rats.


METHODS
Three adult male SD rats were used to isolated olfactory bulb and primarily cultured OECs. In the 8th or 9th day, OECs were transplanted, the concentration of cells was modulated to 1 x 10(5)/microL. Fifty-four SD rats were made the models of T8 spinal cord crush injury and divided into 4 groups. In group A (control, n = 36), injured site was not treated; in groups B, C and D (n = 6), OECs, ChABC and OECs+ChABC were injected into injured site, respectively. At 1, 2, 3, 7 and 14 days after injury, the BBB score system was used to evaluate the motion function. At 0, 1, 2, 3, 7, 14 days in group A and at 14 days in groups B, C, D after injury, the maximal transverse diameter and gross area of necrosis were evaluated on HE stained sections. The immunofluorescence double labeling staining for glial-fibrillary acidic protein (GFAP)/CS56, GFAP/growth associated protein 43 (GAP-43) and GFAP/neurofilament 160 (NF160) was carried out to evaluate the regeneration of nerve fiber.


RESULTS
At 14 days after injury, there were significant difference in the BBB scores between group A and groups B, C, D (P < 0.05), and between groups B, C and group D (P < 0.05), HE staining showed that the formation of cavity was observed in each group at 14 days after injury. There were significant difference in the maximal transverse diameter and gross area of necrosis between groups B, C, D and group A (P < 0.01), and between groups B, C and group D (P < 0.01). The immunofluorescence staining indicated that expression of GFAP were more intense in group A than in other groups, and the cavity of the lesion site was apparent, but it was moderate in groups B and C. The expression of GAP-43 was more intense in group D than in groups B and C. The expression of NF160 was more intense in group D.


CONCLUSIONS
Transplantation strategy of OECs combined with ChABC was effective in the repair of SCI in some extent.
Zhang
Chun
C
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710004, P.R. China.
He
Xijing
X
Lan
Binshang
B
Li
Haopeng
H
D000818:Animals; D018929:Cell Culture Techniques; D017690:Cell Transplantation; D019765:Chondroitin ABC Lyase / Q000627:therapeutic use*; D004195:Disease Models, Animal; D008297:Male; D009416:Nerve Regeneration; D009830:Olfactory Bulb / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000601:surgery / Q000628:therapy*
D019765:Chondroitin ABC Lyase