title: Bioinspired mineralization and cell adhesion on surface functionalized poly(vinyl alcohol) films.
abstract: Poly(vinyl alcohol) (PVA) films, when surface functionalized by phosphorylation, induced biomimetic nucleation and growth of calcium phosphate in a simulated physiological environment. The surface phosphorylation on PVA was ensured by attenuated total reflectance infrared spectroscopy. The morphology of the calcium phosphate phase grown on surface-phosphorylated PVA (PPVA) was analysed using scanning electron microscopy coupled with an energy-dispersive X-ray detector. The primary nucleation of calcium phosphate occurs in 3 days and secondary nucleation occurs after 10 days. The energy-dispersive X-ray analysis shows that the Ca/P ratio of the coating increases with time of exposure to the simulated physiological fluid and reaches 1.67 at 10 days. The PPVA supports in vitro cell adhesion and promotes in vitro biomineralization in the presence of cells, evaluated using human osteosarcoma cells.
authors.affiliation: Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, Kerala, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Sailaja
authors.forename: K
authors.initials: K
authors.lastname: Sreenivasan
authors.forename: Y
authors.initials: Y
authors.lastname: Yokogawa
authors.forename: T V
authors.initials: TV
authors.lastname: Kumary
authors.forename: H K
authors.initials: HK
authors.lastname: Varma
mesh_terms: D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D048430:Cell Shape; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010766:Phosphorylation; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013194:Staining and Labeling; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D011142:Polyvinyl Alcohol; D017886:Durapatite