title: Novel method for determination of substance P levels in unextracted human plasma by using acidification.
abstract: Substance P (SP) is a member of the tachykinin family and has an important role in immune responses. SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction.
authors.affiliation: Joseph Stokes Jr. Research Institute at the Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA. campbelld@email.chop.edu
authors.forename: Donald E
authors.initials: DE
authors.lastname: Campbell
authors.forename: Paula
authors.initials: P
authors.lastname: Bruckner
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Tustin
authors.forename: Richard
authors.initials: R
authors.lastname: Tustin
authors.forename: Steven D
authors.initials: SD
authors.lastname: Douglas
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma / Q000737:chemistry*; D013373:Substance P / Q000032:analysis*
chemical_list: D013373:Substance P