title: Osmium complexation of mismatched DNA: effect of the bases adjacent to mismatched 5-methylcytosine.
abstract: The efficiency of osmium complex formation at 5-methylcytosine in mismatched DNA duplexes is a key point for the design of sequence-specific detection of DNA methylation. Osmium complexation was not observed in fully matched duplexes, whereas the complexation site and efficiency in mismatched duplexes changed depending on the type of 5'-neighboring base of the 5-methylcytosine forming a mismatched base pair. In particular, when the base adjacent to the 5' side of the mismatched base pair was thymine, a unique "side reaction" was observed. However, the nature of the mismatched base pairs in the reaction site did not influence the selectivity of osmium complex formation with methylated DNA.
authors.affiliation: Advanced Science Institute, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nomura
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tainaka
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry*; D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D009992:Osmium; D044503:5-Methylcytosine; D004247:DNA