Effects of parenteral glutamine supplementation on modulating the immune response in rats undergoing a total gastrectomy.
The present study investigated the effect of parenteral glutamine (Gln) supplementation on cellular adhesion molecule expression and release of chemokines responsible for inflammatory cell recruitment in rats undergoing a total gastrectomy. Normal rats with internal jugular catheters were assigned to one control group and two experimental groups and received total parenteral nutrition (TPN). A total gastrectomy was performed in the experimental groups, whereas the control group received a sham operation (Sham). The TPN solutions were isonitrogenous and identical in nutrient composition except that the Sham group and one of the experimental group received conventional (Conv) TPN solution, whereas the other experimental group received 25% of the amino acid nitrogen as Gln. Half of the rats in each group were killed 1 or 3 d after surgery or the Sham to examine their immune response. The results showed that the surgery produced higher polymorphonuclear leucocyte CD11b/CD18 expressions, and Gln supplementation lowered CD11b/CD18 expressions compared with the Conv group post-operatively. The levels of monocyte chemotactic protein-1 and macrophage inflammatory protein-2 in peritoneal lavage fluid were higher in the Gln group than those in the Conv group 1 d post-operatively; these chemotactic proteins had returned to the levels comparable with those in the Sham group on post-operative day 3. These results suggest that Gln supplementation attenuated polymorphonuclear leucocyte integrin expression. In addition, Gln-enriched parenteral nutrition induced an earlier more intensive and rapid immune response to injury than the Conv parenteral nutrition after a total gastrectomy.
Lin
Ming-Tsan
MT
Department of Surgery, College of Medicine, National Taiwan University Hospital and National Taiwan University, Taipei, Taiwan, ROC.
Chou
Szu-Yuan
SY
Tsou
Shung-Sheng
SS
Wang
Ming-Yang
MY
Wu
Ming-Hsun
MH
Yeh
Sung-Ling
SL
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D039481:CD11b Antigen / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology; D018932:Chemokine CCL2 / Q000032:analysis; D054360:Chemokine CXCL1 / Q000032:analysis; D054426:Chemokine CXCL2 / Q000032:analysis; D005743:Gastrectomy; D005973:Glutamine / Q000008:administration & dosage*; D007114:Immunization; D018836:Inflammation Mediators / Q000032:analysis*; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D010289:Parenteral Nutrition, Total / Q000379:methods*; D011184:Postoperative Period; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D039481:CD11b Antigen; D018821:CD18 Antigens; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; C513297:Cxcl1 protein, rat; D018836:Inflammation Mediators; D005973:Glutamine; D015847:Interleukin-4; D007371:Interferon-gamma