Bioinformatics analyses of the mannose-binding lectins from Polygonatum cyrtonema, Ophiopogon japonicus and Liparis noversa with antiproliferative and apoptosis-inducing activities.
In the present study, three typical monocot mannose-binding lectins (e.g., Polygonatum cyrtonema lectin [PCL], Ophiopogon japonicus lectin [OJL] and Liparis noversa lectin [LNL]), were reported to possess a similar tertiary structure with three mannose-binding sites and a close phylogenetic relationship. Subsequently, these lectins were found to bear remarkable inhibitory effects on the growth of MCF-7 cells. Further experiments confirmed that there is a link among the hemagglutinating activity, antiproliferative activity and mannose-binding activity. In addition, these lectins were shown to induce MCF-7 cell apoptosis and caspase was found to be involved in this apoptotic pathway. In conclusion, these findings demonstrate that the different antiproliferative effects may be due to the conserved motifs of mannose-binding sites. Furthermore, our results demonstrate that these lectins induce apoptosis in MCF-7 cells via a caspase-dependent pathway.
Liu
Bo
B
College of Life Sciences & State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China.
Peng
Hao
H
Yao
Qing
Q
Li
Jing
J
Van Damme
Els
E
Balzarini
Jan
J
Bao
Jin-ku
JK
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019295:Computational Biology; D006801:Humans; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D031441:Ophiopogon / Q000737:chemistry*; D010802:Phylogeny; D031384:Polygonatum / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D037241:Mannose-Binding Lectins