title: Effect of arsenic on p53 mutation and occurrence of teratogenic salamanders: their potential as ecological indicators for arsenic contamination.
abstract: The p53 mutation in salamanders can be used as an indicator of arsenic contamination. The influence of arsenic exposure was studied on mutation of tumor suppressor gene in salamanders collected from several As-contaminated mine areas in Korea. Salamander eggs and larvae were exposed to arsenic in a toxicity test, and teratogenic salamanders found in heavy metal- and As-contaminated water from As-Bi mines were evaluated using PCR-SSCP to determine if they would be useful as an ecological indicator species. Changes in amino acids were shown to have occurred as a result of an arsenic-accumulating event that occurred after the DNA damage. In addition, both of the Hynobius leechii exposed groups were primarily affected by forms of skin damage, changes in the lateral tail/dorsal flexure and/or abnormality teratogenesis. Single-base sense mutation in codons 346 (AAG: Lys to ATG: Met), 224 (TTT: Phe to TTA: Leu), 211 (ATG: Met to AAG: Lys), 244 (TTT: Phe to TTTG: insertion), 245 (Glu GAG to Gln CAG) and 249 (TGT Cys to TGA stop) of the p53 gene were simultaneously found in mutated salamanders. Based on the results of our data illustrating the effect of arsenic exposure on the p53 mutation of salamanders in arsenic-contaminated mine areas, these mutated salamanders can be used as potential ecological indicators in the arsenic-contaminated ecosystems.
authors.affiliation: Arsenic Geoenvironment Laboratory (National Research Laboratory), Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Buk-gu, Gwangju, Republic of Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Chang
authors.forename: Man Bock
authors.initials: MB
authors.lastname: Gu
authors.forename: Kyoung-Woong
authors.initials: KW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001151:Arsenic / Q000032:analysis / Q000633:toxicity*; D020261:Arsenic Poisoning / Q000235:genetics*; D004249:DNA Damage; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity; D008906:Mining; D009154:Mutation; D010063:Ovum / Q000187:drug effects / Q000254:growth & development; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D013723:Teratogens / Q000032:analysis / Q000633:toxicity*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014562:Caudata / Q000002:abnormalities / Q000196:embryology / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D012989:Soil Pollutants; D013723:Teratogens; D016159:Tumor Suppressor Protein p53; D014874:Water Pollutants, Chemical; D001151:Arsenic