title: The first extracellular domain of the tumour stem cell marker CD133 contains an antigenic ganglioside-binding motif.
abstract: Prominin 1/CD133 is a marker of transplantable cancer stem cells. We have generated anti-peptide antibodies against a N-terminal epitope of CD133 belonging to a ganglioside-binding domain. The labelling of colon cancer cells with these antibodies was inhibited by various gangliosides including GM1 and GD3, but not GT1b. CD133 immunolabelling progressively decreased to undetectable levels in post-confluent cultures, possibly through ganglioside-mediated epitope masking since the staining was partially recovered after chemical disruption of lipid rafts. We suggest that selected gangliosides could modulate the accessibility of CD133 and regulate cell-cell contacts involving CD133(+) stem cells at the earliest steps of tumour development.
authors.affiliation: CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
authors.forename: Nadira
authors.initials: N
authors.lastname: Taïeb
authors.affiliation: CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Maresca
authors.affiliation: CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Guo
authors.affiliation: CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Garmy
authors.affiliation: CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Fantini
authors.affiliation: CRN2M, CNRS UMR 6231, INRA USC 2027, Laboratoire des Interactions Moléculaires et Systèmes Membranaires, University of Aix-Marseille 2 and Aix-Marseille 3, Faculté des Sciences de St-Jérôme, 13397 Marseille Cedex 20, France. Electronic address: nouara.yahi@univ-cezanne.fr.
authors.forename: Nouara
authors.initials: N
authors.lastname: Yahi
mesh_terms: D000071916:AC133 Antigen; D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000032:analysis; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D005677:G(M1) Ganglioside / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D021962:Membrane Microdomains / Q000502:physiology; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D005732:Gangliosides; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D005677:G(M1) Ganglioside; C026226:ganglioside, GD3