Lipopolysaccharide elicits expression of immune-related genes in the silkworm, Bombyx mori.
Lipopolysaccharide (LPS), a major cell wall component of gram-negative bacteria, was found to be unable to activate immune-related genes in Drosophila melanogaster. In contrast, highly purified LPS elicited immune-related gene expression in the fat body of Bombyx mori. However, the level of activation by highly purified LPS was lower than crude LPS and peptidoglycan. Furthermore, synthetic lipid A also activated these genes, suggesting that B. mori possesses unknown signal pathways to activate immune-related genes by LPS. Up-regulation of antimicrobial peptide genes by highly purified LPS was not confirmed in the immune-responsive cell line, NIAS-Bm-aff3, suggesting that some factors necessary for signal transduction activated by LPS are deficient in this cell line.
Tanaka
H
H
Innate Immunity Research Unit, National Institute of Agrobiological Sciences, Ibaraki, Japan.
Sagisaka
A
A
Fujita
K
K
Kaneko
Y
Y
Imanishi
S
S
Yamakawa
M
M
D000818:Animals; D012831:Bombyx / Q000187:drug effects* / Q000276:immunology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D017344:Genes, Insect / Q000235:genetics*; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*
D008050:Lipid A; D008070:Lipopolysaccharides