Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate the L(beta)-L(alpha) and L(alpha)-H(II) phospholipid phase transitions as well as check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodological combination should allow the identification of essential regions of membrane-interacting proteins which might be implicated in the molecular mechanism of membrane fusion and/or budding.
Pérez-Berná
Ana J
AJ
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Alicante, Spain.
Pabst
George
G
Laggner
Peter
P
Villalaín
José
J
D000595:Amino Acid Sequence; D002152:Calorimetry, Differential Scanning; D016174:Hepacivirus / Q000737:chemistry; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D053838:Scattering, Small Angle; D020539:Sequence Analysis, Protein; D013696:Temperature; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*; D014961:X-Ray Diffraction
D019151:Peptide Library; D010455:Peptides; D014764:Viral Proteins