title: Thymosin beta-10 is aberrantly expressed in pancreatic cancer and induces JNK activation.
abstract: Thymosin beta-10 (T beta 10) has been shown to be associated with several cancers; however, its role in pancreatic cancer is not understood. The expression of T beta 10 was determined by immunohistochemistry and real-time polymerase chain reaction. The phosphorylation of JNK and the cytokine secretion was determined by using the Bio-Plex phosphoprotein and cytokines assays. Pancreatic cancer tissues and cells expressed higher amounts of T beta 10 than normal surrounding tissues and human pancreatic duct epithelial cells. Exogenous T beta 10 caused the phosphorylation of JNK and increased the secretion of cytokines interleukin (IL)-7 and IL-8 in BxPC-3 cells. T beta 10 might be a promising marker and a novel therapeutic target for pancreatic cancer.
authors.affiliation: Molecular Surgeon Research Center, Elkins Pancreas Center, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA. minli@bcm.edu
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qihui
authors.initials: Q
authors.lastname: Zhai
authors.forename: Louis W
authors.initials: LW
authors.lastname: Feurino
authors.forename: William E
authors.initials: WE
authors.lastname: Fisher
authors.forename: Changyi
authors.initials: C
authors.lastname: Chen
authors.forename: Qizhi
authors.initials: Q
authors.lastname: Yao
mesh_terms: D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis; D004789:Enzyme Activation; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology; D010190:Pancreatic Neoplasms / Q000276:immunology* / Q000473:pathology; D010766:Phosphorylation; D013947:Thymosin / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D016207:Cytokines; D013947:Thymosin; C039302:thymosin beta(10); D048031:JNK Mitogen-Activated Protein Kinases