title: Monocyte-derived dendritic cells from patients with severe forms of chromoblastomycosis induce CD4+ T cell activation in vitro.
abstract: Dendritic cells (DCs) have been described as initiators and modulators of the immune response. Recently we have shown a predominant production of interleukin-10 cytokine, low levels of interferon-gamma and inefficient T cell proliferation in patients with severe forms of chromoblastomycosis. Chromoblastomycosis starts with subcutaneous inoculation of Fonsecaea pedrosoi into tissue where DCs are the first line of defence against this microorganism. In the present study, the interaction of F. pedrosoi and DCs obtained from patients with chromoblastomycosis was investigated. Our results showed that DCs from patients exhibited an increased expression of human leucocyte antigen D-related (HLA-DR) and co-stimulatory molecules. In the presence of conidia, the expression of HLA-DR and CD86 was up-regulated by DCs from patients and controls. Finally, we demonstrate the reversal of antigen-specific anergy and a T helper type 1 response mediated by DCs incubated with F. pedrosoi conidea.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de Sao Paulo, SP, Brazil.
authors.forename: M Glória
authors.initials: MG
authors.lastname: Sousa
authors.forename: E Eid Bou
authors.initials: EE
authors.lastname: Ghosn
authors.forename: R Ciciro
authors.initials: RC
authors.lastname: Nascimento
authors.forename: G Facchioli
authors.initials: GF
authors.lastname: Bomfim
authors.forename: V
authors.initials: V
authors.lastname: Noal
authors.forename: K
authors.initials: K
authors.lastname: Santiago
authors.forename: C
authors.initials: C
authors.lastname: de Maria Pedrozo E Silva Azevedo
authors.forename: S Garcia
authors.initials: SG
authors.lastname: Marques
authors.forename: A Guedes
authors.initials: AG
authors.lastname: Gonçalves
authors.forename: D Wagner
authors.initials: DW
authors.lastname: de Castro Lima Santos
authors.forename: P Ricardo
authors.initials: PR
authors.lastname: Criado
authors.forename: J Eduardo
authors.initials: JE
authors.lastname: Costa Martins
authors.forename: S Rogerio
authors.initials: SR
authors.lastname: Almeida
mesh_terms: D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000276:immunology; D051940:B7-2 Antigen / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002862:Chromoblastomycosis / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D003904:Mitosporic Fungi / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology
chemical_list: D000946:Antigens, Fungal; D051940:B7-2 Antigen; D016207:Cytokines; D006684:HLA-DR Antigens