Role of silencing phosphatase of regenerationg liver-3 expression by microRNA interference in the growth of gastric cancer.
BACKGROUND
Accumulated evidences demonstrate that phosphatase of regeneration liver-3 (PRL-3) is associated with metastasis of multiple tumor types, and has been validated as a potential therapeutic target for metastasis. High expression of PRL-3 was implicated in lymph node metastasis of gastric cancer. In the present study, we investigated the role of silencing PRL-3 expression by microRNA (miRNA) interference in gastric cancer growth.


METHODS
RNA interference, mediated by recombinant lentivirus expressing artificial PRL-3 miRNA, was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and tumor growth.


RESULTS
Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm(3), significantly smaller compared with controls (P < 0.001).


CONCLUSIONS
Knockdown of PRL-3 significantly suppressed the proliferation of SGC7901 cells in vitro and tumor growth in vivo. PRL-3 plays a key role in the growth of gastric cancer. PRL-3 should be considered as a potential therapeutic target.
Cai
Shi-rong
SR
Department of Gastrointestinal Surgery, First Affiliated Hospital, Sun Yat-sen University, Gastric Cancer Center of Sun Yat-sen University, Guangzhou, Guangdong, China.
Wang
Zhao
Z
Chen
Chuang-qi
CQ
Wu
Wen-hui
WH
He
Yu-long
YL
Zhan
Wen-hua
WH
Zhang
Chang-hua
CH
Cui
Ji
J
Wu
Hui
H
D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
D035683:MicroRNAs; D009363:Neoplasm Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases