Monitoring autophagy in insect eggs.
Oogenesis is a fundamental physiological process in insects. Successful oogenesis is critical for evolutionary success by transferring genetic information to the next generation. This is achieved by the normal maturation of the egg chamber (egg), which is accomplished through cell death of the cells that accompany the oocyte. Recent studies demonstrate that autophagy contributes to this cell death process. Hence, comprehension of the mechanisms that implicates autophagy during cell death in insect eggs is very important. Herein, we describe some experimental approaches that can be used to monitor autophagy in insect eggs.
Nezis
Ioannis P
IP
Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
Papassideri
Issidora
I
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007313:Insecta / Q000166:cytology / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D009866:Oogenesis / Q000502:physiology
D005456:Fluorescent Dyes; D019476:Insect Proteins