Detection of acetyl monoglyceride as a metabolite of newly isolated glycerol-assimilating bacteria.
Thirty-five glycerol-assimilating bacteria have recently been isolated from soil samples. Amplified ribosomal DNA restriction analysis revealed that these strains are grouped into four genetically different types of bacteria. Gas chromatography-mass spectrometry analysis of glycerol metabolites produced by the three selected strains (strains HH7, HH12, and HH31) revealed that extracts of culture liquid with ethyl acetate contains acetyl monoglyceride (monoacetin), which has not previously been reported as a glycerol metabolite and is used as a solvent, plasticizer, and food additive, as well as for other industrial purposes. The sequence analyses of the 16S rRNA genes from the selected strains showed that all of them belong to the Enterobacteriaceae.
Habe
Hiroshi
H
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. hiroshi.habe@aist.go.jp
Iwabuchi
Hiroyuki
H
Uemura
Shingo
S
Tamura
Takamitsu
T
Morita
Tomotake
T
Fukuoka
Tokuma
T
Imura
Tomohiro
T
Sakaki
Keiji
K
Kitamoto
Dai
D
D004755:Enterobacteriaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005503:Food Additives / Q000378:metabolism*; D005989:Glycerides / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012997:Solvents / Q000378:metabolism*
D005503:Food Additives; D005989:Glycerides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012997:Solvents; C038923:monoacetin; D005990:Glycerol