title: Isolation and identification of two novel SDS-resistant secreted chitinases from Aeromonas schubertii.
abstract: Two SDS-resistant endochitinases, designated as ASCHI53 and ASCHI61, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI-TOF mass measurement indicates the molecular weights of 53,527 for ASCHI53 and 61,202 for ASCHI61. N-terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCHI53 and ASCHI61 retained, respectively, 65% and 75%, of their chitinase activity in the presence of 5% SDS and 100% of their activity in the presence of 10% beta-mercaptoethanol. These results demonstrate that they are SDS-resistant endochitinases and probably have a rigid structure.
authors.affiliation: Graduate School of Biochemical Engineering, and Dept. of Safety Health and Environmental Engineering, MingChi University of Technology, Taipei, Taiwan, Republic of China. f2402002@ms16.hinet.net
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Liu
authors.forename: Chia-Rui
authors.initials: CR
authors.lastname: Shen
authors.forename: Fong-Fu
authors.initials: FF
authors.lastname: Hsu
authors.forename: Jeen-Kuan
authors.initials: JK
authors.lastname: Chen
authors.forename: Pei-Tzu
authors.initials: PT
authors.lastname: Wu
authors.forename: Shang-Hsin
authors.initials: SH
authors.lastname: Guo
authors.forename: Wen-Chien
authors.initials: WC
authors.lastname: Lee
authors.forename: Feng-Wei
authors.initials: FW
authors.lastname: Yu
authors.forename: Zachary B
authors.initials: ZB
authors.lastname: Mackey
authors.forename: John
authors.initials: J
authors.lastname: Turk
authors.forename: Michael L
authors.initials: ML
authors.lastname: Gross
mesh_terms: D000333:Aeromonas / Q000201:enzymology*; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D002688:Chitinases / Q000302:isolation & purification* / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate; D002688:Chitinases