Physical training leads to remodeling of diaphragm muscle in asthma model.
Matrix metalloproteinases (MMPs) are crucial to the development and maintenance of healthy tissue and are mainly involved in extracellular matrix (ECM) remodeling of skeletal muscle. This study evaluated the effects of chronic allergic airway inflammation (CAAI), induced by ovalbumin, and aerobic training in the MMPs activity in mouse diaphragm muscle. Thirty mice were divided into 6 groups: 1) control; 2) ovalbumin; 3) treadmill trained at 50% of maximum speed; 4) ovalbumin and trained at 50%; 5) trained at 75%; 6) ovalbumin and trained at 75%. CAAI did not alter MMPs activities in diaphragm muscle. Nevertheless, both treadmill aerobic trainings, associated with CAAI increased the MMP-2 and -1 activities. Furthermore, MMP-9 was not detected in any group. Together, these findings suggest an ECM remodeling in diaphragm muscle of asthmatic mice submitted to physical training. This result may be useful for a better understanding of functional significance of changes in the MMPs activity in response to physical training in asthma.
Durigan
J L Q
JL
Department of Physical Therapy, Federal University of São Carlos, Brazil. joaodurigan@gmail.com
Peviani
S M
SM
Russo
T L
TL
Duarte
A C
AC
Vieira
R P
RP
Martins
M A
MA
Carvalho
C R F
CR
Salvini
T F
TF
D000818:Animals; D001249:Asthma / Q000201:enzymology / Q000503:physiopathology / Q000534:rehabilitation*; D003964:Diaphragm / Q000201:enzymology / Q000378:metabolism*; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism; D010047:Ovalbumin; D010805:Physical Conditioning, Animal
D010047:Ovalbumin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1