Rrp2, a sigma54-dependent transcriptional activator of Borrelia burgdorferi, activates rpoS in an enhancer-independent manner.
Rrp2 is the sole sigma(54)-dependent transcriptional activator present in the Borrelia burgdorferi genome. We showed that recombinant Rrp2 binds to DNA in a sequence-nonspecific manner. During infection, Rrp2 activates sigma(54)-dependent rpoS expression without an apparent upstream enhancer element commonly associated with other sigma(54)-dependent transcriptional activators.
Blevins
Jon S
JS
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Xu
Haijun
H
He
Ming
M
Norgard
Michael V
MV
Reitzer
Larry
L
Yang
X Frank
XF
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D025065:Borrelia burgdorferi / Q000502:physiology*; D004269:DNA, Bacterial / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051877:RNA Polymerase Sigma 54 / Q000378:metabolism; D012808:Sigma Factor / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; D015534:Trans-Activators; C069567:sigma factor KatF protein, Bacteria; D051877:RNA Polymerase Sigma 54