Molecularly imprinted solid-phase extraction for the selective determination of 17beta-estradiol in fishery samples with high performance liquid chromatography.
A molecularly imprinted polymer (MIP) has been synthesized by a thermo-polymerization method using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, acetonitrile as porogenic solvent, and 17beta-estradiol as template. The MIP showed obvious affinity for 17beta-estradiol in acetonitrile solution, which was confirmed by absorption experiments. After optimization of molecularly imprinted solid-phase extraction (MISPE) conditions, three structurally related estrogenic compounds (17beta-estradiol, estriol, and diethylstilbestrol) were used to evaluate the selectivity of the MIP cartridges. The MIP cartridges exhibited highly selectivity for E(2), the recoveries were 84.8+/-6.53% for MIPs and 19.1+/-1.93% for non-imprinted polymer (NIP) cartridges. The detection and quantification limits correspond to 0.023 and 0.076 mg L(-1). Furthermore, the MISPE methods were used to selectively extract E(2) from fish and prawn tissue prior to HPLC analysis. This MISPE-HPLC procedure could eliminate all matrix interference simultaneously and had good recoveries (78.3-84.5%).
Jiang
Tianhe
T
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Zhao
Lixia
L
Chu
Baolin
B
Feng
Qinzhong
Q
Yan
Wei
W
Lin
Jin-Ming
JM
D000818:Animals; D004958:Estradiol / Q000032:analysis*; D005398:Fisheries / Q000379:methods / Q000592:standards*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D008689:Methacrylates; D054802:Molecular Imprinting; D033561:Penaeidae; D012758:Shellfish; D052616:Solid Phase Extraction / Q000379:methods*
D008689:Methacrylates; C008384:methacrylic acid; D004958:Estradiol; C004919:ethylene dimethacrylate