The C-terminus of the P22 tailspike protein acts as an independent oligomerization domain for monomeric proteins.
TSP (P22 tailspike protein) is a well-established model system for studying the folding and assembly of oligomeric proteins, and previous studies have documented both in vivo and in vitro folding intermediates using this protein. Especially important is the C-terminus of TSP, which plays a critical role in the assembly and maturation of the protrimer intermediate to its final trimeric form. In the present study, we show that by grafting the C-terminus of TSP on to the monomeric MBP (maltose-binding protein), the resulting chimaera (MBP-537) is a trimeric protein. Moreover, Western blot studies (using an anti-TSP antibody) indicate that the TSP C-terminus in the MBP-537 chimaera has the same conformation as the native TSP. The oligomerization of the MBP-537 chimaera appears to involve hydrophobic interactions and a refolding sequence, both of which are analogous to the native TSP. These results underscore the importance of the TSP C-terminus in the assembly of the mature trimer and demonstrate its potential utility as a model to study the folding and assembly of the TSP C-terminus in isolation.
Webber
Tawnya
T
Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, AZ 86011, U.S.A.
Gurung
Sarsati
S
Saul
Justin
J
Baker
Trenton
T
Spatara
Michelle
M
Freyer
Matthew
M
Robinson
Anne Skaja
AS
Gage
Matthew J
MJ
D000906:Antibodies / Q000276:immunology; D017100:Bacteriophage P22 / Q000737:chemistry*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases; D057927:Hydrophobic and Hydrophilic Interactions; D058619:Maltose-Binding Proteins; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D017510:Protein Folding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017376:Viral Tail Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D050505:Mutant Proteins; D011993:Recombinant Fusion Proteins; D017376:Viral Tail Proteins; D006026:Glycoside Hydrolases; C083268:tailspike protein, bacteriophage