title: Isolation and characterization of human coronary artery-derived endothelial cells in vivo from patients undergoing percutaneous coronary interventions.
abstract: OBJECTIVE
  Human coronary artery-derived endothelial cells (ECs) seem to be the most appropriate cells for the pathogenesis study of coronary artery disease. But limited availability of endothelial tissue is a major constraint. In this study, we developed a method to isolate human coronary artery ECs in vivo from patients.
  
  
  METHODS
  Coronary guidewires were used to obtain EC samples from coronary arteries in 76 patients. Cells were eluted from wire tips and purified by immunomagnetic beads. Von Willebrand factor and CD31 were used as immunocytochemical markers to identify cells as endothelium. Cell viability was evaluated in terms of cell membrane integrity, energy-dependent uptake of DiI-labeled acetylated low-density lipoprotein, and apoptosis. Nitric oxide synthase (eNOS) expression and nitric oxide (NO) production of cells were detected to evaluate cell function.
  
  
  RESULTS
  About 96 coronary artery ECs were obtained per guidewire. Cells manifested endothelial morphology and immunoreactivity for von Willebrand factor and CD31 with good viability. But eNOS expression and NO production of cells were decreased.
  
  
  CONCLUSIONS
  Viable coronary endothelium could be obtained during routine percutaneous coronary interventions combined with immunomagnetic beads. These cells may be used for advanced cellular functional analyses such as immunocytochemistry and molecular biology. Such information could aid in understanding mechanisms of coronary artery diseases.
authors.affiliation: Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Yu
authors.forename: Yao-Ming
authors.initials: YM
authors.lastname: Song
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Li
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Yu
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Ming-Bao
authors.initials: MB
authors.lastname: Song
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Lin
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Tao
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
mesh_terms: D000368:Aged; D015906:Angioplasty, Balloon, Coronary; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D051929:CD146 Antigen / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation / Q000379:methods*; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D015415:Biomarkers; D051929:CD146 Antigen; C497902:MCAM protein, human; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III