title: Expression of maturation markers on murine dendritic cells in response to group A streptococcal lipopeptide vaccines.
abstract: Bone marrow (BM)-FMS-like tyrosine kinase 3 (Flt3) ligand-murine dendritic cells (DC) in response to group A streptococcal (GAS) lipopeptide vaccines containing an analogue of a Toll-like receptor (TLR) 2 agonist showed significant up-regulation in expression of DC maturation markers CD40 and CD80 when compared to unstimulated controls. There were significant increases in MHC class II, CD40, CD80 and CD86 expression on classical DC and plasmacytoid DC after in vivo administration or in vitro stimulation of BM-granulocyte macrophage colony stimulating factor (GMCSF)-DC with lipopolysaccharide but not lipopeptide GAS vaccines. Our results indicate that an LCP-GAS vaccine induced phenotypic maturation of BM-Flt3-DC but not BM-GMCSF-DC.
authors.affiliation: The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane, Queensland 4029, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Yong
authors.forename: Daniel
authors.initials: D
authors.lastname: Mitchell
authors.forename: Audrey
authors.initials: A
authors.lastname: Caudron
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
authors.forename: Colleen
authors.initials: C
authors.lastname: Olive
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000502:physiology; D003713:Dendritic Cells / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D055666:Lipopeptides / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D013154:Spleen / Q000276:immunology; D022541:Streptococcal Vaccines / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*; D051941:fms-Like Tyrosine Kinase 3 / Q000502:physiology
chemical_list: D015415:Biomarkers; D055666:Lipopeptides; D022541:Streptococcal Vaccines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C497971:Flt3 protein, mouse; D051941:fms-Like Tyrosine Kinase 3