[Studies on preparative technology and quantitative determination for extracts of total saponin in roof of Panax japonicus].
OBJECTIVE
To explore the optimum extraction and purification condition of the total saponins in the root of Panax japonicus (RPJ), and establish its quality control methods.


METHODS
Designed L16 (4(5)) orthogonal test with the extraction rate of total saponins as index, to determine the rational extraction process, and the techniques of water-saturated n-butanol extraction and acetone precipitation were applied to purify the alcohol extract of RPJ. Total saponins were detected by spectrophotometry and its triterpenoidal sapogenin oleanolic acid detected by HPLC.


RESULTS
The optimum conditions of total saponins from RPJ was as follows: the material was pulverized, dipped in 60% ethanol aqueous solution as extract solvent at 10 times of volume, and refluxed 3 times for 3 h each time. Extractant of water-saturated n-butanol with extraction times of 3 and precipitant of acetone with precipitation amount of 4-5 times were included in the purification process, which would obtain the quality products. The content of total saponins could reach to 83.48%, and oleanolic acid to 38.30%.


CONCLUSIONS
The optimized preparative technology is stable, convenient and practical. The extract rate of RPJ was high and steady with this technology, which provided new evidence for industrializing production of the plant and developing new drug.
He
Yu-min
YM
Medical Science College of China Three Gorges University, Yichang 443002, China.
Lu
Ke-ming
KM
Yuan
Ding
D
Zhang
Chang-cheng
CC
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D012503:Saponins