Coronin 1 is essential for IgM-mediated Ca2+ mobilization in B cells but dispensable for the generation of immune responses in vivo.
Coronin 1 is a leukocyte specific regulator of Ca(2+)-dependent signaling and is essential for the survival of peripheral T lymphocytes, but its role in B cells is unknown. In this study, we show that coronin 1 is essential for intracellular Ca(2+) mobilization and proliferation upon triggering of the BCR. However, the presence of costimulatory signals rendered coronin 1 dispensable for B cell signaling, consistent with the generation of normal immune responses against a variety of Ags in coronin 1-deficient mice. We conclude that coronin 1, while being essential for T cell function and survival, is dispensable for B cell function in vivo.
Combaluzier
Benoit
B
Biozentrum, University of Basel, Basel, Switzerland.
Mueller
Philipp
P
Massner
Jan
J
Finke
Daniela
D
Pieters
Jean
J
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007075:Immunoglobulin M / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000276:immunology* / Q000378:metabolism; D018613:Microscopy, Confocal
D007075:Immunoglobulin M; D008840:Microfilament Proteins; C072061:coronin proteins; D002118:Calcium