title: Production of FK520 by Streptomyces tubercidicus.
abstract: The discovery of immunosuppressant compounds created the conditions for the successful transplantations. Effective immunosuppressant compounds were isolated from cultures of different microorganisms, among others macrolide type immunosuppressants, FK506 and FK520, were isolated from the fermentation broths of Streptomyces species. In this study a screening program was carried out to isolate microorganisms, which produce macrolide type immunosuppressant compounds. More than 40,000 actinomycete strains were investigated in the screening program comprising chemical, microbiological and PCR methods for the investigation of the cultures. Actinomycete strains with FK520 biosynthesizing ability were isolated in the program, which were identified as isolates of the Streptomyces tubercidicus species according to the taxonomical investigations. A part of the 31-O-demethyl-FK520 methyltransferase gene of the isolated S. tubercidicus was sequenced and it showed 88% homology to that of Streptomyces hygroscopicus. Till now strains of the Streptomyces hygroscopicus species were known to produce FK520 and this study proved that strains of other Streptomyces species have FK520 biosynthetic ability.
authors.affiliation: IVAX Drug Research Institute Ltd., 1045 Budapest Berlini ùt 47-49, Hungary. h8773kon@ella.hu
authors.forename: Attila
authors.initials: A
authors.lastname: Kónya
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Szabó
authors.forename: Ildikó
authors.initials: I
authors.lastname: Láng
authors.forename: István
authors.initials: I
authors.lastname: Barta
authors.forename: János
authors.initials: J
authors.lastname: Salát
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000378:metabolism*; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016559:Tacrolimus / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D007166:Immunosuppressive Agents; D018942:Macrolides; D003577:Cytochrome P-450 Enzyme System; C101653:FK506 macrolide 9-hydroxylase, Streptomyces; D016559:Tacrolimus