title: [Sperm-fluorescence in situ hybridization analysis in patients with pericentric inversions of Y chromosome].
abstract: OBJECTIVE
  To analyze the sex chromosome meiotic segregation in inv(Y) patients by fluorescence in situ hybridization (FISH).
  
  
  METHODS
  Conventional cytogenetic procedures (GTG and CBG banding) and FISH were performed on metaphase chromosome. Three-color FISH was performed on sperm samples using a probe mixture containing CEPX, Tel Xp/Yp and Tel Xq/Yq to investigate the sex chromosome segregation of five inv(Y) (p11.1q11.2) carriers. A healthy man with normal semen parameters was used as control.
  
  
  RESULTS
  There was no statistical difference in the abnormal sex chromosome number and recombination frequencies in each spermatozoon from the patient in comparison with that in the control.
  
  
  CONCLUSIONS
  There was no apparent sex chromosome abnormality in the sperm of the inv(Y) (p11.1q11.2) carriers. Sperm-FISH allows further understanding of the sex chromosome segregation pattern and an accurate genetic counseling.
authors.affiliation: Department of Clinical Laboratory, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006 PR China.
authors.forename: Yu-qin
authors.initials: YQ
authors.lastname: Luo
authors.forename: Yu-li
authors.initials: YL
authors.lastname: Qian
authors.forename: Huan-ming
authors.initials: HM
authors.lastname: Lu
authors.forename: Chen-ming
authors.initials: CM
authors.lastname: Xu
authors.forename: Fan
authors.initials: F
authors.lastname: Jin
mesh_terms: D016022:Case-Control Studies; D007446:Chromosome Inversion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology