title: Polyphosphoric acid treatment promotes bone regeneration around titanium implants.
abstract: This study was conducted to evaluate the effect of polyphosphoric acid (PPA) treatment on bone regeneration around titanium (Ti) implants in vivo. Adsorption of PPA by Ti was achieved by immersing Ti implants (2 mm in diameter, 4 mm in length) in different concentrations of PPA solution (0, 1 and 10 wt%) for 24 h at 37 degrees C after proper Ti surface cleaning. The treated Ti implants were implanted on 8-week-old-male rat (n = 30) tibiae. Two or four weeks after implantation, all animals were deeply anaesthetized and underwent perfusion fixation. Ten specimens in each condition were further immersed in the same fixative for 1 week and eventually embedded in polyester resin. Afterwards, undecalcified sections were ground to a thickness of approximately 70 microm parallel to the long axis of the implant. The sections were stained with basic fuchsine and methylene blue and then examined by light microscopy. For quantitative evaluation of bone regeneration around the implants, the bone-implant contact ratio (BICR) was determined. Polyphosphoric acid treatment of the Ti implant surface significantly enhanced direct bone contact to the Ti surface. Especially, the BICRs of the 1 wt% PPA-treated Ti implants were significantly higher than those of the control untreated Ti implants, both 2 and 4 weeks after implantation. At 4 weeks, 10 wt% PPA-treated implants also significantly increased the BICR as compared to that of the untreated Ti implants. These results suggest that PPA treatment promotes osteoconductivity of Ti in vivo.
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Shikata-cho, Okayama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maekawa
authors.forename: K
authors.initials: K
authors.lastname: Shimono
authors.forename: M
authors.initials: M
authors.lastname: Oshima
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: B
authors.initials: B
authors.lastname: Van Meerbeek
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kuboki
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D020007:Implants, Experimental; D008297:Male; D016348:Osseointegration / Q000187:drug effects; D010756:Phosphoric Acids / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000033:anatomy & histology / Q000187:drug effects / Q000601:surgery; D014025:Titanium
chemical_list: D010756:Phosphoric Acids; D011108:Polymers; C047269:polyphosphoric acid; D014025:Titanium