title: Differentially expressed parasite genes involved in host recognition and invasion of the triactinomyxon stage of Myxobolus cerebralis (Myxozoa).
abstract: The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-activated and in vitro-activated TAMs were compared by suppressive subtractive hybridization (SSH) to identify parasite genes involved in the host invasion process. Differential screening and a subsequent BLAST search revealed 15 of 452 SSH-library clones expressed differently in activated TAMs. None of the 15 transcripts obtained has previously been identified from M. cerebralis. Quantitative real-time PCR was used to examine the relative expression profile of 8 selected transcripts upon TAM activation and after penetration of the host. Four of these were found to be up-regulated in activated TAMs, while expression was relatively low in non-activated TAMs and in infected fish tissue, indicating that they are relevant genes during host recognition or subsequent host invasion of M. cerebralis TAMs.
authors.affiliation: Clinic of Fish and Reptiles, Faculty of Veterinary Medicine, University of Munich, Kaulbachstrasse 37, D-80539 Munich, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Eszterbauer
authors.forename: D M
authors.initials: DM
authors.lastname: Kallert
authors.forename: D
authors.initials: D
authors.lastname: Grabner
authors.forename: M
authors.initials: M
authors.lastname: El-Matbouli
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D005393:Fish Diseases / Q000469:parasitology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions; D055766:Myxobolus / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D009693:Nucleic Acid Hybridization / Q000379:methods; D017686:Oncorhynchus mykiss / Q000469:parasitology*; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D033761:Spores, Protozoan / Q000502:physiology
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins