A multimodal platform for nonlinear optical microscopy and microspectroscopy.
Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.
Chen
Hongtao
H
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Wang
Haifeng
H
Slipchenko
Mikhail N
MN
Jung
YooKyung
Y
Shi
Yunzhou
Y
Zhu
Jiabin
J
Buhman
Kimberly K
KK
Cheng
Ji-Xin
JX
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D005247:Feeding Behavior; D007834:Lasers; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000502:physiology; D008853:Microscopy / Q000379:methods*; D015591:Microspectrophotometry / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D017711:Nonlinear Dynamics; D013059:Spectrum Analysis, Raman; D040521:Subcutaneous Tissue / Q000502:physiology; D014025:Titanium / Q000737:chemistry; D014732:Vibration
D008055:Lipids; C009495:titanium dioxide; D014025:Titanium