title: Prenatal diagnosis of beta-thalassemia/Hb E by hemoglobin typing compared to DNA analysis.
abstract: To determine the accuracy of prenatal diagnosis of beta-thalassemia (beta-thal)/Hb E disease using fetal hemoglobin (Hb) typing compared to DNA analysis, automated DNA sequencing was performed on 98 blood samples from fetuses diagnosed as beta-thal/Hb E by Hb typing. Thirteen samples from homozygous Hb E fetuses were also collected. The Hb patterns obtained by high performance liquid chromatography (HPLC) from both groups were analyzed. The codon 26 (G>A) mutation was identified in all 98 samples. The beta-globin gene mutation was identified in 97 cases by DNA sequencing and the 3.4 kb deletion by polymerase chain reaction (PCR) in one case. The result from DNA analysis was in agreement with the HPLC result in all samples. In beta-thal/Hb E fetuses, the Hb A level was 0-0.3% and mean Hb A(2)(E) level was 1.3 +/- 0.3%. In homozygous Hb E fetuses, the Hb A level was 0% and mean Hb A(2)(E) level was 2.48 +/- 0.6%. The Hb pattern obtained by HPLC on fetal blood is a reliable and accurate method for prenatal diagnosis of this disease.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. ssiricho@mail.med.cmu.ac.th
authors.forename: Supatra
authors.initials: S
authors.lastname: Sirichotiyakul
authors.forename: Rattika
authors.initials: R
authors.lastname: Saetung
authors.forename: Torpong
authors.initials: T
authors.lastname: Sanguansermsri
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004252:DNA Mutational Analysis; D005260:Female; D005319:Fetal Hemoglobin; D006441:Hemoglobin A / Q000032:analysis; D006443:Hemoglobin A2 / Q000032:analysis; D006446:Hemoglobin E / Q000032:analysis* / Q000235:genetics; D006720:Homozygote; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000235:genetics
chemical_list: D006441:Hemoglobin A; D006443:Hemoglobin A2; D006446:Hemoglobin E; D005319:Fetal Hemoglobin