title: Ontogeny of FOXP3(+) regulatory T cells in the postnatal human small intestinal and large intestinal lamina propria.
abstract: FOXP3(+) regulatory T cells (Treg) suppress innate and adaptive immune responses and are critical for intestinal immune homeostasis. Our objective was to define the postnatal developmental regulation of Treg in relationship to other T cells in the human intestinal tract. We analyzed 41 small and 18 large intestinal paraffin-embedded tissue samples from preterm and term infants with and without necrotizing enterocolitis (NEC) for the presence of CD3(+), CD4(+), CD8(+), and FOXP3(+) cells by immunohistochemistry. We compared labeled cells against age, gestational age (GA), or (corrected) postmenstrual age (PMA). The GA ranged from 23 to 40 weeks, with a mean of 32 (standard deviation, 4.7) weeks. Independent of age, GA, or PMA, the numbers of CD4(+) cells were higher in the small intestine compared to the large intestine (P = 0.046), except in patients with NEC. FOXP3(+) cells could be detected as early as 23 weeks in GA in both large and small bowel, and similar quantities were detected at the highest GA examined (40 weeks). We saw no statistically significant effect of GA, age, or PMA on total number of FOXP3(+) cells or by comparing FOXP3(+) to CD4(+) or FOXP3(+) to CD8(+) ratios, indicating intact ontogeny of Treg in intestinal tissue early in gestation. Human infants exhibit presence of mucosal FOXP3(+) cells in the small and large intestinal mucosa at birth and as early as 23 weeks GA. The frequency of FOXP3(+) cells and the ratios of FOXP3(+) to CD4(+) or CD8(+) cells do not change with increasing intrauterine development or postnatal age.
authors.affiliation: Department of Pediatrics, Division of Neonatology, Monroe Carell Jr. Children's Hospital at Vanderbilt, 2215 B Garland Avenue, 1125 MRB IV/Light Hall, Nashville, TN 37232-0656, USA. hendrik.weitkamp@vanderbilt.edu
authors.forename: Jörn-Hendrik
authors.initials: JH
authors.lastname: Weitkamp
authors.forename: Erin
authors.initials: E
authors.lastname: Rudzinski
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Koyama
authors.forename: Hernan
authors.initials: H
authors.lastname: Correa
authors.forename: Pranathi
authors.initials: P
authors.lastname: Matta
authors.forename: Brannon
authors.initials: B
authors.lastname: Alberty
authors.forename: D-Brent
authors.initials: DB
authors.lastname: Polk
mesh_terms: D020345:Enterocolitis, Necrotizing / Q000276:immunology; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology* / Q000378:metabolism; D005865:Gestational Age; D006801:Humans; D018928:Immunity, Mucosal / Q000502:physiology; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007413:Intestinal Mucosa / Q000254:growth & development / Q000276:immunology*; D007420:Intestine, Large / Q000254:growth & development / Q000276:immunology; D007421:Intestine, Small / Q000254:growth & development / Q000276:immunology; D008297:Male; D009092:Mucous Membrane / Q000254:growth & development / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors