11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum.
Abstract 11beta-Hydroxysteroid dehydrogenase (11beta-OHSD) metabolizes corticosterone to inactive 11-dehydrocorticosterone and thus protects non-specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11beta-OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11beta-OHSD bioactivity with similar co-substrate requirements and inhibition kinetics to the renal enzyme. 11beta-OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11beta-OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.
Moisan
M P
MP
Department of Medicine, Western General Hospital, Edinburgh EH4 2XU, UK.
Seckl
J R
JR
Brett
L P
LP
Monder
C
C
Agarwal
A K
AK
White
P C
PC
Edwards
C R
CR