Study of the coumarate decarboxylase and vinylphenol reductase activities of Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) isolates.
OBJECTIVE
To evaluate the coumarate descarboxylase (CD) and vinylphenol reductase (VR) activities in Dekkera bruxellensis isolates and study their relationship to the growth rate, protein profile and random amplified polymorphic DNA (RAPD) molecular pattern.


RESULTS
CD and VR activities were quantified, as well, the growth rate, intracellular protein profile and molecular analysis (RAPD) were determined in 12 isolates of D. bruxellensis. All the isolates studied showed CD activity, but only some showed VR activity. Those isolates with the greatest growth rate did not present a different protein profile from the others. The FASC showed a relationship between RAPD molecular patterns and VR activity.


CONCLUSIONS
CD activity is common to all of the D. bruxellensis isolates. This was not the case with VR activity, which was detected at a low percentage in the analysed micro-organisms. A correlation was observed between VR activity and the RAPD patterns.


CONCLUSIONS
This is the first study that quantifies the CD and VR enzyme activities in D. bruxellensis, demonstrating that these activities are not present in all isolates of this yeast.
Godoy
L
L
Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Alameda, Santiago, Chile.
Garrido
D
D
Martínez
C
C
Saavedra
J
J
Combina
M
M
Ganga
M A
MA
D001709:Biotechnology; D055168:Brettanomyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D003470:Culture Media; D055167:Dekkera / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D014920:Wine / Q000382:microbiology
D003373:Coumaric Acids; D003470:Culture Media; D005656:Fungal Proteins; D010636:Phenols; D010088:Oxidoreductases; D002262:Carboxy-Lyases; C030626:4-vinylphenol