title: Nocardia jinanensis sp. nov., an amicoumacin B-producing actinomycete.
abstract: A novel actinomycete, strain 04-5195(T), that produces amicoumacin B, which targets bone morphogenetic protein-2, was isolated from a soil sample collected in Jinan, Shandong Province, China. Strain 04-5195(T) had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia and it formed a phyletic line in the Nocardia 16S rRNA gene tree. It was evident from the phylogenetic data that strain 04-5195(T) was most closely associated with Nocardia speluncae N2-11(T). However, the two organisms were distinguishable from one another using DNA-DNA relatedness and phenotypic data. The isolate was readily differentiated from other related Nocardia strains by a set of phenotypic properties and by its phylogenetic position. Therefore, it is proposed that the isolate represents a novel species in the genus Nocardia, Nocardia jinanensis sp. nov.; the type strain is 04-5195(T) (=CGMCC 4.3508(T) =DSM 45048(T)).
authors.affiliation: The National Key Laboratory for Screening New Microbial Drugs, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Zhao-Yong
authors.initials: ZY
authors.lastname: Yang
authors.forename: Zhi-Heng
authors.initials: ZH
authors.lastname: Liu
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D003374:Coumarins; D012336:RNA, Ribosomal, 16S; C539460:amicoumacin B