Methods to monitor autophagy of Salmonella enterica serovar Typhimurium.
Autophagy is an important component of the mammalian innate immune system and is able to specifically target intracellular bacterial pathogens. Salmonella enterica serovar Typhimurium is an intracellular pathogen that causes gastroenteritis in humans. Autophagy has been shown to target S. Typhimurium during in vitro infection of mammalian cultured cells and protects the cytosol of these cells from bacterial colonization. Here we discuss autophagic sequestration of S. Typhimurium and how it can be used as a model system to study the mechanisms of autophagy.
Birmingham
Cheryl L
CL
Department of Molecular Genetics and Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada.
Brumell
John H
JH
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D000071183:Autophagy-Related Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D003424:Crohn Disease / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D051379:Mice; D008954:Models, Biological; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000378:metabolism; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism
C518959:ATG16L1 protein, human; D000071183:Autophagy-Related Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins