title: A fluorescence scanning electron microscope.
abstract: Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.
authors.affiliation: Morphology and Core Unit, Kyushu University Hospital, Kyushu, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kanemaru
authors.forename: Kazuho
authors.initials: K
authors.lastname: Hirata
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Takasu
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Isobe
authors.forename: Keiji
authors.initials: K
authors.lastname: Mizuki
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Mataka
authors.forename: Kei-Ichiro
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D051381:Rats; D017208:Rats, Wistar