Differential microRNA expression in childhood B-cell precursor acute lymphoblastic leukemia.
MiRNAs play important roles in the development of both hematopoiesis and leukemogenesis. The analysis of differential microRNA expression profiles may be a powerful tool to allow us insight on the mechanisms of childhood B-cell precursor acute lymphoblastic leukemia (pre-B-ALL). The present study provides an informative profile of the expression of miRNAs in pre-B-ALL using two independent and quantitative methods: miRNA chip and qRT-PCR of mature miRNA from 40 newly diagnosed pre-B-ALL children. Additionally, putative hematopoiesis-specific target genes were analyzed with informatics technique. Both approaches showed that miR-222, miR-339, and miR-142-3p were dramatically overexpressed in pre-B-ALL patients, and downregulation of hsa-miR-451 and hsa-miR-373* was confirmed. The results of this study offer a comprehensive and quantitative profile of miRNA expression in pre-B-ALL and their healthy counterpart, suggesting that miRNAs could play a primary role in the disease itself.
Ju
Xiuli
X
Cryomedicine Laboratory, Qilu Hospital of Shandong University, Shandong, China. shellysdcn07@hotmail.com
Li
Dong
D
Shi
Qing
Q
Hou
Huaishui
H
Sun
Nianzheng
N
Shen
Baijun
B
D002648:Child; D002675:Child, Preschool; D019295:Computational Biology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D007223:Infant; D035683:MicroRNAs / Q000235:genetics*; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics
D035683:MicroRNAs; C525710:MIR222, human; C527114:MIRN373 microRNA, human; C531201:MIRN451 microRNA, human