The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles.
Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.
Zhang
Qi
Q
Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.
Li
Yulong
Y
Tsien
Richard W
RW
D000818:Animals; D002478:Cells, Cultured; D004558:Electric Stimulation; D006624:Hippocampus / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport; D049449:Luminescence; D008561:Membrane Fusion; D009474:Neurons / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D017729:Presynaptic Terminals / Q000502:physiology; D045663:Quantum Dots; D051381:Rats; D017207:Rats, Sprague-Dawley; D013570:Synaptic Membranes / Q000502:physiology; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000502:physiology*
D018377:Neurotransmitter Agents