title: Novel cell lines derived by long-term culture of primary uveal melanomas.
abstract: OBJECTIVE
  Uveal melanomas with or without monosomy 3 have different metastatic potentials. Therefore, it would be of great experimental importance to obtain cell lines of both groups - established and characterized under the same conditions.
  
  
  METHODS
  Long-term culture of biopsies derived from untreated primary uveal melanomas was performed, and the status of chromosome 3 in the tumour was determined. Characterization by immunocytochemistry and in vitro assays for cell migration, vasculogenic cord formation and proliferation were performed.
  
  
  RESULTS
  Establishment of cell lines succeeded in 6 out of 128 uveal melanomas. No histopathological feature was predictive for cultivation success. Two out of 4 cell lines derived from tumours with monosomy 3 were invasive in vitro. Both cell lines with disomy 3 showed higher proliferation levels (p = 0.002).
  
  
  CONCLUSIONS
  The in vitro tendency for invasion did not correlate with the proliferative behaviour of uveal melanoma cell lines, but was partly associated with monosomy of chromosome 3.
authors.affiliation: Department of Ophthalmology, Universitätsklinikum Essen, Essen, Germany. gordon.nareyeck@uni-due.de
authors.forename: Gordon
authors.initials: G
authors.lastname: Nareyeck
authors.forename: Michael
authors.initials: M
authors.lastname: Zeschnigk
authors.forename: Norbert
authors.initials: N
authors.lastname: Bornfeld
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Anastassiou
mesh_terms: D001706:Biopsy; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D002470:Cell Survival; D002893:Chromosomes, Human, Pair 3; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000556:secondary*; D009006:Monosomy; D009362:Neoplasm Metastasis; D014602:Uvea / Q000473:pathology*; D014604:Uveal Neoplasms / Q000235:genetics / Q000473:pathology*