title: Genetic risk factors for anterior cruciate ligament ruptures: COL1A1 gene variant.
abstract: BACKGROUND
  Anterior cruciate ligament (ACL) ruptures are considered the most severe injury sustained in sports. Although various intrinsic and extrinsic risk factors have been identified, the exact aetiology of the injury is not yet fully understood. Recently, the gene encoding for the alpha1 chain of type I collagen (COL1A1) has been shown to be associated with cruciate ligament ruptures and shoulder dislocations.
  
  
  OBJECTIVE
  To determine whether the functional Sp1 binding site polymorphism within intron 1 of the COL1A1 gene is associated specifically with ACL ruptures in an independent population.
  
  
  METHODS
  117 Caucasian participants with surgically diagnosed ACL ruptures, and 130 Caucasian physically active controls without any history of previous ligament or tendon injuries were recruited for this case-control genetic association study. All participants were genotyped for the COL1A1 Sp1 binding site polymorphism (G/T; rs1800012).
  
  
  RESULTS
  The rare TT genotype was significantly (p = 0.031, OR = 0.08, 95% CI <0.01 to 1.46) under-represented in the ACL group (0 out of 117, 0%), compared with the controls (6 out of 130, 4.6%).
  
  
  CONCLUSIONS
  The TT genotype of the COL1A1 Sp1 binding site polymorphism was significantly under-represented in South African participants with ACL ruptures. We propose that this sequence variant be the first specific genetic element to be included in multifactorial models developed to understand the aetiology and risk factors for ACL rupture.
authors.affiliation: UCT/MRC Research Unit for Exercise Science and Sports Medicine, Newlands 7725, Cape Town, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Posthumus
authors.forename: A V
authors.initials: AV
authors.lastname: September
authors.forename: M
authors.initials: M
authors.lastname: Keegan
authors.forename: D
authors.initials: D
authors.lastname: O'Cuinneagain
authors.forename: W
authors.initials: W
authors.lastname: Van der Merwe
authors.forename: M P
authors.initials: MP
authors.lastname: Schwellnus
authors.forename: M
authors.initials: M
authors.lastname: Collins
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000070598:Anterior Cruciate Ligament Injuries; D001265:Athletic Injuries / Q000235:genetics*; D001665:Binding Sites / Q000235:genetics; D016022:Case-Control Studies; D004247:DNA / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D012421:Rupture / Q000235:genetics
chemical_list: C120892:COLIA1-PDGFB fusion protein, human; D015514:Oncogene Proteins, Fusion; D004247:DNA