Effectiveness of small interfering RNA (siRNA) against the Mcl-1 gene in a canine mammary gland tumor cell line.
The effect of down-regulation of Mcl-1 expression by small interfering RNA (siRNA) against the canine Mcl-1 gene on apoptosis was investigated by transfecting CF33 (canine mammary gland tumor cell line) with siRNA using cationic liposomes. The siRNA against canine Mcl-1 increased the rate of apoptotic cells and decreased the numbers of viable cells. Further, sequence-specific down-regulation of Mcl-1 expression was measured by real time-PCR and Western blot analysis. The siRNA directed against the Mcl-1 gene reduced both the mRNA and protein expression in the CF33. Our study suggests the importance of Mcl-1 in canine mammary tumors for inducing apoptosis and reinforces using Mcl-1 as a putative therapeutic target in canine mammary gland tumor.
Kano
Rui
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. kano@brs.nihon-u.ac.jp
Yano
Takayuki
T
Nagamatsu
Kota
K
Maruyama
Haruhiko
H
Kamata
Hiroshi
H
Hasegawa
Atsuhiko
A
D000818:Animals; D045744:Cell Line, Tumor; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008321:Mammary Glands, Animal / Q000166:cytology*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology*
D009363:Neoplasm Proteins; D034741:RNA, Small Interfering