title: Identification and characterization of class 1 integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China.
abstract: OBJECTIVE
  The role of integrons in the spread of antibiotic resistance has been well established. The aim of this study was to investigate the resistance profiles of Pseudomonas aeruginosa isolated from patients in Zhenjiang to 13 antibiotics, and to identify the structure and dissemination of class 1 integrons.
  
  
  METHODS
  The Kirby-Bauer disk diffusion assay was used to determine the rate of P. aeruginosa resistance. Class 1 integrons from multidrug-resistant isolates were amplified by PCR, and their PCR products were sequenced. We also analyzed the integron structures containing the same gene cassettes by restriction fragment length polymorphism (RFLP). Isolates were genotyped by pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  The resistance rates were between 29.6% and 90.1%. The prevalence of class 1 integrons was 38.0%. These integrons included five gene cassettes (aadB, aac6-II, blaPSE-1, dfrA17, and aadA5). The dfrA17 and aadA5 gene cassettes were found most often.
  
  
  CONCLUSIONS
  Class 1 integrons were found to be widespread in P. aeruginosa isolated from clinical samples in the Zhenjiang area of China. The antibiotic resistance rates in class 1 integron-positive strains of P. aeruginosa were noticeably higher than those in class 1 integron-negative strains. PFGE showed that particular clones were circulating among patients.
authors.affiliation: Clinical Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
authors.forename: Yingzhao
authors.initials: Y
authors.lastname: Liu
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Dai
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Li
authors.forename: Sufang
authors.initials: S
authors.lastname: Peng
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Shao
authors.forename: Haifang
authors.initials: H
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wen
authors.forename: Jianren
authors.initials: J
authors.lastname: Yu
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial