title: The effect of laminin peptide gradient in enzymatically cross-linked collagen scaffolds on neurite growth.
abstract: Guided neurite growth is critical in both peripheral nervous system and central nervous system nerve regeneration. Scaffolds that provide structural and guidance cues for neuronal cells have a potential role in neural regeneration application. Type I collagen is suitable to be processed as an engineered scaffold for nerve regeneration because of its biological and structural properties. A few previous studies have shown that cross-linking of collagen scaffolds with microbial transglutaminase improves the mechanical strength and degradation properties of the scaffolds. It was shown that laminin 5 can regulate neurite outgrowth and extension. A motif (PPFLMLLKGSTR) in the human laminin 5 alpha 3 chain is crucial for both integrin alpha 3 beta 1 receptor binding and cell adhesion. In the present work, we studied the guidance effect of a laminin peptide (PPFLMLLKGSTR) gradient in collagen and cross-linked collagen scaffolds on neurite growth. Neurites of rat pheochromocytoma (PC12) cells showed a preferential growth toward the high laminin concentration level on the collagen scaffold, while the incorporation of laminin peptide in the scaffold did not influence neurite length of PC12 cells.
authors.affiliation: National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.forename: Gopinath
authors.initials: G
authors.lastname: Damodaran
authors.forename: Natalia
authors.initials: N
authors.lastname: Nikolskaya
authors.forename: Adrienne M
authors.initials: AM
authors.lastname: Gorman
authors.forename: Anthony
authors.initials: A
authors.lastname: Windebank
authors.forename: Abhay
authors.initials: A
authors.lastname: Pandit
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D007797:Laminin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009416:Nerve Regeneration; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D013306:Streptomycetaceae / Q000201:enzymology; D054457:Tissue Scaffolds / Q000737:chemistry*; D011503:Transglutaminases / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D007797:Laminin; D003094:Collagen; D011503:Transglutaminases; D016650:Fluorescein-5-isothiocyanate