[Identification of heterologous antitoxin in sera of patients with diphtheria].
Using immobilized diphtheria toxin and peroxidase conjugate of monoclonal antibodies to light chains of equine immunoglobulin a method of quantification of equine antibodies against diphtheria in sera of patients after serotherapy was developed. The sensitivity of indirect enzyme-linked immunosorbent assay was 0.0005 IU/ml, and coefficient of variation did not exceed 10%. It was shown that in patients with toxic diphtheria heterologous antitoxin is eliminated within 4-6 weeks. Level of anti-diphtheria immunoglobulin under the similar severity of disease and dosage of antitoxin can vary in wide ranges and depends from individual's characteristics.
Gal'vidis
I A
IA
Burkin
M A
MA
Sviridov
V V
VV
D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood; D000911:Antibodies, Monoclonal; D003353:Corynebacterium diphtheriae / Q000276:immunology*; D004165:Diphtheria / Q000097:blood* / Q000628:therapy*; D004166:Diphtheria Antitoxin / Q000097:blood*; D004167:Diphtheria Toxin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006736:Horses / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive / Q000592:standards; D007147:Immunoglobulin Light Chains / Q000097:blood
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D004166:Diphtheria Antitoxin; D004167:Diphtheria Toxin; D007147:Immunoglobulin Light Chains