title: Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis.
abstract: 2,3-Diketo-5-methylthiopentyl-1-phosphate enolase (DK-MTP-1P enolase) from Bacillus subtilis was crystallized using the hanging-drop vapour-diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 A resolution at 100 K using synchrotron radiation and were found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 79.3, b = 91.5, c = 107.0 A, beta = 90.8 degrees. The asymmetric unit contained four molecules of DK-MTP-1P enolase, with a V(M) value of 2.2 A(3) Da(-1) and a solvent content of 43%.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Tamura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ashida
authors.forename: Shogo
authors.initials: S
authors.lastname: Koga
authors.forename: Yohtaro
authors.initials: Y
authors.lastname: Saito
authors.forename: Tomonori
authors.initials: T
authors.lastname: Yadani
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kai
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Akiho
authors.initials: A
authors.lastname: Yokota
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010755:Organophosphates / Q000737:chemistry*; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry / Q000235:genetics
chemical_list: C519707:2,3-diketo-5-methylthiopentane 1-phosphate; D001426:Bacterial Proteins; D010755:Organophosphates; D011994:Recombinant Proteins; D012273:Ribulose-Bisphosphate Carboxylase; D010751:Phosphopyruvate Hydratase