title: Comparative analysis of four lipoproteins from Mycoplasma mycoides subsp. mycoides Small Colony identifies LppA as a major T-cell antigen.
abstract: Control of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), remains an important goal in Africa. Subunit vaccines triggering B and T-cell responses could represent a promising approach. To this aim, the T-cell immunogenicity of four MmmSC lipoproteins (LppA, LppB, LppC and LppQ), present in African strains and able to elicit humoral response, was evaluated. In vitro assays revealed that only LppA was recognized by lymph node lymphocytes taken from three cattle, 3 weeks after MmmSC exposure. Maintenance of the LppA-specific response, relying on CD4 T-cells and IFN gamma production, was then demonstrated 1 year after infection. LppA is thus an important target for the CD4 T-cells generated early after MmmSC infection and persisting in the lymph nodes of recovered cattle. Its role as a protective antigen and ability to in vivo trigger both arms of the host immune response remain to be evaluated.
authors.affiliation: CIRAD, UMR Contrôle des Maladies, F-34398 Montpellier, France. laurence.dedieu@cirad.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Dedieu
authors.forename: Philippe
authors.initials: P
authors.lastname: Totte
authors.forename: Valerie
authors.initials: V
authors.lastname: Rodrigues
authors.forename: Edy M
authors.initials: EM
authors.lastname: Vilei
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology; D049109:Cell Proliferation; D015964:Gene Expression Regulation, Bacterial / Q000276:immunology*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D009176:Mycoplasma mycoides / Q000378:metabolism*; D011002:Pleuropneumonia, Contagious / Q000276:immunology / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117984:LPPA protein, Mycoplasma mycoides subsp.; D008074:Lipoproteins