Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata.
The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis oculata.
Min
Joong Kee
JK
Department of Biotechnology, Soonchunhyang University, Asan, Chungnan-336-600, Korea.
Rhim
Tae Yeon
TY
Shin
Hyun Woong
HW
Lee
Mi Young
MY
D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure; D056890:Eukaryota / Q000187:drug effects* / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D015854:Up-Regulation / Q000187:drug effects*
D017931:DNA Primers; D002104:Cadmium; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase