Cell-culture-based immunochromatography for rapid detection of group A human rotaviruses in aquatic environments.
In an effort to detect rapidly and conveniently the infectious waterborne group A human rotaviruses that cause diarrhea in infants and children, the authors developed the first-ever cell-culture-based immunochromatography method. An immunochromatography kit interacted only with the human rotaviruses VP6 antigen, and the cell-culture-based immunochromatography detected group A human rotaviruses with a sensitivity as low as 1.99 TCID50 ml(-1). This detection sensitivity was similar to that of the cytopathic effect-based method. There were no actual differences between the sensitivity of this method and that of the real-time reverse transcription polymerase chain reaction method, which is known as a method with a relatively high sensitivity. Furthermore, while cell culture detection methods, that is total culturable virus assay, can determine only the presence of infectious waterborne viruses, the cell-culture-based immunochromatography is advantageous for the accurate detection of group A human rotaviruses. Compared with the real-time reverse transcription polymerase chain reaction method, the cell-culture-based immunochromatography is advantageous because it requires a relatively simple process that enables easy quality controls and low test costs. Thus, this study proposed a new method for the identification of group A human rotaviruses, and it is suggested that this cell-culture-based immunochromatography may be applied to detect group A human rotaviruses in aquatic environments.
Lee
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GC
Water Analysis and Research Center, Korea Institute of Water and Environment, Korea Water Resources Corporation, Daejeon, Republic of Korea. gclee@kwater.or.kr
Chong
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CK
Lee
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CH
Lee
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ST
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000956:Antigens, Viral / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002845:Chromatography / Q000379:methods; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D014871:Water Microbiology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic; C061332:VP6 protein, Rotavirus