title: Ultra-high-pressure liquid chromatography-tandem mass spectrometry for the analysis of six resorcylic acid lactones in bovine milk.
abstract: This work reports a rapid, reliable and sensitive multi-residue method for the simultaneous determination of six resorcylic acid lactones in bovine milk by ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The resorcylic acid lactones were extracted, purified, and concentrated from milk samples in one step using a solid-phase extraction (SPE) cartridge that contained a polymeric mixed-mode anion-exchange sorbent. The analysis was performed on a Waters Acquity BEH C(18) column utilizing a gradient elution profile. Each LC run was completed in 3.5 min. The analytes were detected by multiple reaction monitoring (MRM) using electrospray ionization (ESI) negative mode. Mean recoveries from fortified samples ranged from 92.6% to 112.5%, with relative standard deviations lower than 11.4%. Using 5 mL bovine milk, the limits of detection and quantification for resorcylic acid lactones were in the ranges of 0.01-0.05 and 0.05-0.2 microg/L, respectively. The application of this newly developed method was demonstrated by analyzing bovine milk samples from markets.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.forename: Shuangyang
authors.initials: S
authors.lastname: Ding
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Li
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004968:Estrogens, Non-Steroidal / Q000032:analysis; D007783:Lactones / Q000032:analysis*; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012118:Resorcinols / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015029:Zeranol / Q000032:analysis
chemical_list: D004968:Estrogens, Non-Steroidal; D007783:Lactones; D012118:Resorcinols; D015029:Zeranol