Synthesis of hepcidin derivatives in order to develop standards for immune adsorption method.
MeCN, acetonitrile; ECL, enhanced chemiluminescence; EDT, 1,2-ethanedithiole; HEPC12-A, rabbit anti-human hepcidin IgG, affinity purified; HEPC13-A, rabbit anti-mouse/human hepcidin IgG, affinity purified; HEPC61-P, human hepcidin-25 control/blocking synthetic peptide; HRP, horseradish peroxidase; IL-6, interleukin-6; KLH, keyhole limpet hemocyanin; LEAP, liver-expressed antimicrobial peptide; NEM, N-ethylmaleimide; NMP, N-methyl-pirrolidone; PBS, phosphate buffered saline; PVDF, polyvinylidene difluoride; SELDI-TOF-MS, surface-enhanced laser desorption ionization-time-of-flight mass spectrometry; TMB, tetramethylbenzidin; TNF-alpha, tumor necrosis factor-alpha.
Balogh
Adám
A
First Department of Pediatrics, Semmelweis University, Budapest, Hungary. balad@gyer1.sote.hu
Horváti
Kata
K
Mezo
Gábor
G
Derzbach
László
L
Szebeni
Beáta
B
Nagy
Lajos
L
Prechl
József
J
Vásárhelyi
Barna
B
Hudecz
Ferenc
F
Bosze
Szilvia
S
D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000097:blood / Q000138:chemical synthesis* / Q000737:chemistry / Q000592:standards; D019921:Biotinylation; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005033:Ethylmaleimide; D064451:Hepcidins; D006801:Humans; D007163:Immunosorbent Techniques / Q000592:standards; D008969:Molecular Sequence Data; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D023181:Antimicrobial Cationic Peptides; C578702:HAMP protein, human; C579599:Hamp protein, mouse; D064451:Hepcidins; D005033:Ethylmaleimide