title: Enhancement of in vivo anticancer effects of cisplatin by incorporation inside single-wall carbon nanohorns.
abstract: Cisplatin (CDDP) was incorporated inside single-wall carbon nanohorns with holes opened (SWNHox) by a nanoprecipitation method that involved dispersion of CDDP and SWNHox in a solvent followed by the solvent evaporation. The incorporated CDDP quantity increased from the previously reported value of 15 to 46%, and the total released quantity of CDDP also increased from 60 to 100% by changing the solvent from dimethylformamide to water. Concurrently, in vitro anticancer efficiency of CDDP@SWNHox increased to 4-6 times greater than that of the intact CDDP. In vivo, CDDP@SWNHox intratumorally injected to transplanted tumors of mice suppressed the tumor growth more than the intact CDDP. We observed that CDDP@SWNHox adhered to the cell surfaces in vitro and stayed within the tumor tissues in vivo. Therefore, we think that the CDDP released from SWNHox realized high concentrations locally at the cells in vitro and in the tissues in vivo and could efficiently attack the tumor cells. We also found that SWNHox itself had an in vivo anticancer effect, which might increase the anticancer activities of CDDP@SWNHox.
authors.affiliation: SORST, Japan Science and Technology Agency c/o NEC, 34 Miyukigaoka, Tsukuba, Ibaraki 305-8501, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ajima
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murakami
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Mizoguchi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Tsuchida
authors.forename: Toshinari
authors.initials: T
authors.lastname: Ichihashi
authors.forename: Sumio
authors.initials: S
authors.lastname: Iijima
authors.forename: Masako
authors.initials: M
authors.lastname: Yudasaka
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage* / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050997:Nanomedicine / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D037742:Nanotubes, Carbon; D002945:Cisplatin