title: Mapping of IgE-binding epitopes on the major latex allergen Hev b 2 and the cross-reacting 1,3beta-glucanase fruit allergens as a molecular basis for the latex-fruit syndrome.
abstract: Nine distinct IgE-binding epitopes were identified along the entire amino acid sequence of the major latex allergen Hev b 2 (1,3beta-glucanase) using a set of synthetic 15-mer peptides frameshifted by 3 residues immobilized on cellulose membrane (Spot technique). Most of the amino acid residues building these IgE-binding epitopic regions are nicely exposed on the surface and the epitopes usually correspond to charged regions on the molecular surface of the protein. A smaller number of 5 IgE-binding epitopic areas was identified on the banana 1,3beta-glucanase, which exhibits a very similar overall conformation and charge distribution. The latter epitopes might be responsible for the IgE-binding cross-reactivity currently observed in the latex-fruit syndrome. Using rabbit polyclonal IgG anti-BanGluc as a probe instead of IgE from allergic patients the same epitopic regions were identified in both Hev b 2 and BanGluc. Additionally, surface-exposed regions with a very close conformation were predicted to occur on Ole e 9, the 1,3beta-glucanase allergen identified in olive pollen.
authors.affiliation: Surfaces Cellulaires et Signalisation chez les Végétaux, UMR Université Paul Sabatier-CNRS 5546, 24 Chemin de Borde Rouge, 31326 Castanet Tolosan, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Barre
authors.forename: Raphaël
authors.initials: R
authors.lastname: Culerrier
authors.forename: Claude
authors.initials: C
authors.lastname: Granier
authors.forename: Laetitia
authors.initials: L
authors.lastname: Selman
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: Françoise
authors.initials: F
authors.lastname: Bienvenu
authors.forename: Jacques
authors.initials: J
authors.lastname: Bienvenu
authors.forename: Pierre
authors.initials: P
authors.lastname: Rougé
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D003429:Cross Reactions / Q000276:immunology; D018604:Epitope Mapping; D005260:Female; D005638:Fruit / Q000276:immunology; D043326:Glucan 1,3-beta-Glucosidase / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D007840:Latex / Q000276:immunology*; D020315:Latex Hypersensitivity / Q000209:etiology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000276:immunology; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013577:Syndrome; D055815:Young Adult
chemical_list: D000485:Allergens; D007840:Latex; D010940:Plant Proteins; D007073:Immunoglobulin E; D043326:Glucan 1,3-beta-Glucosidase