title: Lipopolysaccharide elicits expression of immune-related genes in the silkworm, Bombyx mori.
abstract: Lipopolysaccharide (LPS), a major cell wall component of gram-negative bacteria, was found to be unable to activate immune-related genes in Drosophila melanogaster. In contrast, highly purified LPS elicited immune-related gene expression in the fat body of Bombyx mori. However, the level of activation by highly purified LPS was lower than crude LPS and peptidoglycan. Furthermore, synthetic lipid A also activated these genes, suggesting that B. mori possesses unknown signal pathways to activate immune-related genes by LPS. Up-regulation of antimicrobial peptide genes by highly purified LPS was not confirmed in the immune-responsive cell line, NIAS-Bm-aff3, suggesting that some factors necessary for signal transduction activated by LPS are deficient in this cell line.
authors.affiliation: Innate Immunity Research Unit, National Institute of Agrobiological Sciences, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Sagisaka
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
authors.forename: S
authors.initials: S
authors.lastname: Imanishi
authors.forename: M
authors.initials: M
authors.lastname: Yamakawa
mesh_terms: D000818:Animals; D012831:Bombyx / Q000187:drug effects* / Q000276:immunology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D017344:Genes, Insect / Q000235:genetics*; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides