title: [The activity of thermolability amylase in serum nonsmoking and smoking healthy persons and patients with pancreatitis].
abstract: The aim of this study is to prove the influence of tobacco smoking on total and thermolability amylase activity in the serum of non-smoking and smoking health persons and patients with diagnosed acute (AP), chronic exaggerated (CEP) and chronic pancreatitis (CP) and patients with diabetes. The blood has been collected from 28 healthy persons and 52 patients. The enzyme total activity has been determined using the colorimetric method with substrate 1,2-odilauryl-rac-glycero-3-glutaric acid -(6-methylresorufin) ester. The thermolability activity has been determined using the thermolability test. The tobacco smoke has been examined on the basic of concentration of cotinine in the serum of health persons and patients. The highest amylase total activity and her thermolability form have been found in smoking patients with diabetes. It has been noted that the serum amylase activity is significantly higher in smoking and healthy persons (p < 0.0002; p < 0.002) then in non-smoking and healthy patients. However no significant differences have been found between the thermolability total activity, however it has been noted higher thermolability thermolability activity in smoking patients with CP and nonsmoking patients with CP. Smoking patients with AP and CEP have been found to have a significantly increased enzyme and her form thermolability activity (p > 0.001; p > 0.005 respectively) when compared to non-smoking patients. Results of examination indicate that tobacco smoking has a significant influence on pancreatic amylase activity.
authors.affiliation: Zakład Biomedycznych Analiz Srodowiskowych, Akademia Medyczna we Wrocławiu. msm@tox.am.wroc.pl
authors.forename: Mariola
authors.initials: M
authors.lastname: Sliwińska-Mossoń
authors.forename: Halina
authors.initials: H
authors.lastname: Milnerowicz
mesh_terms: D000328:Adult; D000681:Amylases / Q000097:blood*; D015897:Comorbidity; D003920:Diabetes Mellitus / Q000201:enzymology* / Q000453:epidemiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010195:Pancreatitis / Q000201:enzymology* / Q000453:epidemiology; D050500:Pancreatitis, Chronic / Q000201:enzymology / Q000453:epidemiology; D012016:Reference Values; D044967:Serum / Q000378:metabolism; D012907:Smoking / Q000097:blood* / Q000453:epidemiology
chemical_list: D000681:Amylases