Cloning and characterization of the promoter of the human AHI1 gene.
The AHI1 gene is involved in axons crossing the midline during development, and its mutation can cause Joubert syndrome and other related neurological system diseases. Its transcriptional regulation mechanism is still unknown. Here we cloned and characterized the upstream 5' region of the gene. It was shown that 1.3 kb upstream of ATG had the transcription activity. Progressive deletion analysis was done, and all the constructs were transfected into COS7 cells. The promoter activity was determined by fluorescence microscopy, flow cytometry, and reverse transcription polymerase chain reaction. Our results showed that all the constructs had transcription activity. The highest promoter activity was 30 bp upstream of ATG, which included a transcription factor-binding site for Oct-1. Our research provides an important basis for further investigations on the transcriptional regulation of the human AHI1 gene.
Meng
Xian-Fang
XF
Key Laboratory of Nervous System of Hubei Province, Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China. xmeng@mails.tjmu.edu.cn
Luo
Yi
Y
Xiao
Wei
W
Li
Man
M
Shi
Jing
J
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005434:Flow Cytometry; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1 / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
C487178:AHI1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D050811:Octamer Transcription Factor-1; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins