title: A multimodal platform for nonlinear optical microscopy and microspectroscopy.
abstract: Multimodal nonlinear optical microscopy is a valuable tool to study complex biological samples. We present an easy-to-operate approach to perform coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), second harmonic generation (SHG), and third-harmonic generation (THG) imaging using a single laser source composed of an 80 MHz femtosecond (fs) laser, an optical parametric oscillator (OPO), and a PPLN crystal for frequency doubling. The platform allows vibrationally resonant CARS imaging of CH-rich myelin sheath in fresh spinal tissues and lipid bodies in live cells. Multimodal nonlinear optical imaging and microspectroscopy analysis of fresh liver tissues are demonstrated.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Chen
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Mikhail N
authors.initials: MN
authors.lastname: Slipchenko
authors.forename: YooKyung
authors.initials: Y
authors.lastname: Jung
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Shi
authors.forename: Jiabin
authors.initials: J
authors.lastname: Zhu
authors.forename: Kimberly K
authors.initials: KK
authors.lastname: Buhman
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D005247:Feeding Behavior; D007834:Lasers; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000502:physiology; D008853:Microscopy / Q000379:methods*; D015591:Microspectrophotometry / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D017711:Nonlinear Dynamics; D013059:Spectrum Analysis, Raman; D040521:Subcutaneous Tissue / Q000502:physiology; D014025:Titanium / Q000737:chemistry; D014732:Vibration
chemical_list: D008055:Lipids; C009495:titanium dioxide; D014025:Titanium