Azurin-poly(N-isopropylacrylamide) conjugates by site-directed mutagenesis and their thermosensitive behavior in electron-transfer processes.
Searching for "intelligence": Azurin-PNIPAM conjugates were prepared by site-directed mutagenesis followed by protein reconstitution by using imidazole-conjugated poly(N-isopropylacrylamides). The polymer-bound imidazole acts as a ligand in the active site of the blue copper protein azurin. The bioconjugates showed thermosensitive behavior in electron-transfer processes with reduced cytochrome c.
Rosenberger
Nadine
N
Organisch-Chemisches Institut, Westfälische Wilhelms-Universität, Corrensstrasse 40, 48149 Münster, Germany.
Studer
Armido
A
Takatani
Nobuyuki
N
Nakajima
Hiroshi
H
Watanabe
Yoshihito
Y
D000178:Acrylamides / Q000138:chemical synthesis / Q000737:chemistry*; D000180:Acrylic Resins; D001400:Azurin / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D016297:Mutagenesis, Site-Directed; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D015202:Protein Engineering; D013056:Spectrophotometry, Ultraviolet
D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D001400:Azurin; C052970:poly-N-isopropylacrylamide; D003300:Copper