The immunostimulatory effect of IL-1beta in vivo is blocked by antisense peptides complementary to the loop sequence 163-171.
Antisense (AS) peptides complementary to the beta-bulge surface loop VQGEESNDK (Boraschi loop) of the cytokine interleukin-1beta (IL-1beta) have been shown to bind IL-1beta at the Boraschi loop position, and to inhibit some of the IL-1beta-mediated biological effects in vitro. Here we show that primary AS peptide FVITFFSLY inhibits IL-1beta-mediated immunostimulation in vivo in a dose-dependent fashion, while inactive on IL-1beta-induced inflammation, an effect that takes place independently of the Boraschi loop. To the best of our knowledge, this is the first time that an AS peptide has been used successfully in vivo.
Boraschi
Diana
D
Laboratory of Cytokines, Unit of Immunobiology, Institute of Biomedical Technologies (ITB), National Research Council (CNR), Area della Ricerca di S. Cataldo, Via G. Moruzzi 1, I-56124 Pisa, Italy.
Tagliabue
Aldo
A
Miller
Andrew D
AD
D000276:Adjuvants, Immunologic / Q000235:genetics* / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D002999:Clone Cells; D005260:Female; D007114:Immunization; D053583:Interleukin-1beta / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008954:Models, Biological; D009842:Oligopeptides / Q000235:genetics*; D011485:Protein Binding / Q000235:genetics
D000276:Adjuvants, Immunologic; D053583:Interleukin-1beta; D009842:Oligopeptides