title: Significance of treated agrowaste residue and autochthonous inoculates (Arbuscular mycorrhizal fungi and Bacillus cereus) on bacterial community structure and phytoextraction to remediate soils contaminated with heavy metals.
abstract: In this study, we analyzed the impact of treatments such as Aspergillus niger-treated sugar beet waste (SB), PO4(3-) fertilization and autochthonous inoculants [arbuscular mycorrhizal (AM) fungi and Bacillus cereus], on the bacterial community structure in a soils contaminated with heavy metals as well as, the effectiveness on plant growth (Trifolium repens). The inoculation with AM fungi in SB amended soil, increased plant growth similarly to PO4(3-) addition, and both treatments matched in P acquisition but bacterial biodiversity estimated by denaturing gradient gel electrophoresis of amplified 16S rDNA sequences, was more stimulated by the presence of the AM fungus than by PO4(3-) fertilization. The SB amendment plus AM inoculation increased the microbial diversity by 233% and also changed (by 215%) the structure of the bacterial community. The microbial inoculants and amendment used favoured plant growth and the phytoextraction process and concomitantly modified bacterial community in the rhizosphere; thus they can be used for remediation. Therefore, the understanding of such microbial ecological aspects is important for phytoremediation and the recovery of contaminated soils.
authors.affiliation: Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Profesor Albareda n degrees 1, 18008 Granada, Spain. rosario.azcon@eez.csic.es
authors.forename: Rosario
authors.initials: R
authors.lastname: Azcón
authors.forename: Almudena
authors.initials: A
authors.lastname: Medina
authors.forename: Antonio
authors.initials: A
authors.lastname: Roldán
authors.forename: Borbála
authors.initials: B
authors.lastname: Biró
authors.forename: Astrid
authors.initials: A
authors.lastname: Vivas
mesh_terms: D000383:Agriculture; D001234:Aspergillus niger / Q000378:metabolism; D001409:Bacillus cereus / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D005308:Fertilizers; D019216:Metals, Heavy / Q000032:analysis*; D038821:Mycorrhizae / Q000378:metabolism*; D063245:Plant Development; D018520:Plant Shoots / Q000254:growth & development; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D013559:Symbiosis; D029921:Trifolium / Q000254:growth & development
chemical_list: D005308:Fertilizers; D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants