title: Cloning, expression and immunogenicity of the avian pneumovirus (Colorado isolate) F protein.
abstract: The F protein of the Colorado isolate of avian pneumovirus (APV), expressed from a DNA plasmid, was recognized by antiserum to both A and B subgroup APVs. After two intramuscular injections of turkeys with this plasmid, a homologous antibody response was detected by enzyme-linked immunosorbent assay. This antibody also recognized subgroup A APV. However, there was no neutralization of the Colorado isolate or of subgroup A or B viruses. Although no significant clinical protection was detected following homologous challenge of poults, an anamnestic serological response was seen, suggesting that a systemic antibody response but no local mucosal immunity was induced.
authors.forename: I
authors.initials: I
authors.lastname: Tarpey
authors.forename: M B
authors.initials: MB
authors.lastname: Huggins
authors.forename: P J
authors.initials: PJ
authors.lastname: Davis
authors.forename: R
authors.initials: R
authors.lastname: Shilleto
authors.forename: S J
authors.initials: SJ
authors.lastname: Orbell
authors.forename: J K
authors.initials: JK
authors.lastname: Cook