title: Reversible oxidation of mitochondrial peroxiredoxin 3 in mouse heart subjected to ischemia and reperfusion.
abstract: Peroxiredoxins decompose peroxides through reversible oxidation of their active site cysteines. The redox state of the 2-Cys peroxiredoxins, 1, 2 and 3, was investigated in mouse hearts undergoing ischemia and reperfusion in a Langendorff system. The peroxiredoxins were predominantly reduced in control hearts. Mitochondrial peroxiredoxin 3 underwent significant oxidation to its disulfide-linked dimer during ischemia. Oxidation was largely reversed during reperfusion. No redox changes in cytoplasmic peroxiredoxins 1 and 2 were apparent. Peroxiredoxin 3 oxidation suggests localized mitochondrial generation of reactive oxidants during ischemia. This local antioxidant activity of peroxiredoxin 3 may have a role in maintaining cardiac function.
authors.affiliation: Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.
authors.forename: Vikas
authors.initials: V
authors.lastname: Kumar
authors.forename: Nailya
authors.initials: N
authors.lastname: Kitaeff
authors.forename: Mark B
authors.initials: MB
authors.lastname: Hampton
authors.forename: Mark B
authors.initials: MB
authors.lastname: Cannell
authors.forename: Christine C
authors.initials: CC
authors.lastname: Winterbourn
mesh_terms: D000818:Animals; D005033:Ethylmaleimide / Q000494:pharmacology; D006321:Heart / Q000502:physiology; D051379:Mice; D008929:Mitochondria, Heart / Q000378:metabolism; D017202:Myocardial Ischemia / Q000378:metabolism*; D015425:Myocardial Reperfusion; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction; D061105:Peroxiredoxin III; D054464:Peroxiredoxins / Q000378:metabolism*; D055503:Protein Multimerization / Q000502:physiology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014020:Tissue Extracts / Q000378:metabolism
chemical_list: C516116:Prdx3 protein, mouse; D013439:Sulfhydryl Reagents; D014020:Tissue Extracts; D061105:Peroxiredoxin III; D054464:Peroxiredoxins; D005033:Ethylmaleimide