In Vivo Monitoring of Multiple Circulating Cell Populations Using Two-photon Flow Cytometry.
To detect and quantify multiple distinct populations of cells circulating simultaneously in the blood of living animals, we developed a novel optical system for two-channel, two-photon flow cytometry in vivo. We used this system to investigate the circulation dynamics in live animals of breast cancer cells with low (MCF-7) and high (MDA-MB-435) metastatic potential, showing for the first time that two different populations of circulating cells can be quantified simultaneously in the vasculature of a single live mouse. We also non-invasively monitored a population of labeled, circulating red blood cells for more than two weeks, demonstrating that this technique can also quantify the dynamics of abundant cells in the vascular system for prolonged periods of time. These data are the first in vivo application of multichannel flow cytometry utilizing two-photon excitation, which will greatly enhance our capability to study circulating cells in cancer and other disease processes.
Tkaczyk
Eric R
ER
Center for Ultrafast Optical Science, EECS Department, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI 48109-2099.
Zhong
Cheng Frank
CF
Ye
Jing Yong
JY
Myc
Andrzej
A
Thomas
Thommey
T
Cao
Zhengyi
Z
Duran-Struuck
Raimon
R
Luker
Kathryn E
KE
Luker
Gary D
GD
Norris
Theodore B
TB
Baker
James R
JR