Flow cytometric investigation of CD40-CD40 ligand system in preeclampsia and normal pregnancy.
Our aim was to investigate the CD40-CD40 ligand system in preeclamptic women. We also studied CD62P and platelet-monocyte aggregates, which have been closely linked to the CD40-CD40L system. Platelet expression of CD40L and CD62P and expression of CD40 on monocytes and platelet-monocyte aggregates were determined by flow cytometry in whole blood from 23 preeclamptic women, 23 normotensive pregnant women, and 23 nonpregnant women. The preeclamptic women showed a significant increase in CD40L and CD62P on platelets and in CD40 on monocytes when compared with normotensive pregnant women and nonpregnant women (all P < .001). There was a significant increase in platelet-monocyte aggregates in preeclamptic women (P < .001) and normotensive pregnant women (P = .003) compared with nonpregnant women. Preeclampsia is associated with activation of the CD40-CD40L system. The activation of this system may contribute to the development or maintenance of the proinflammatory and prothrombotic milieu found in preeclampsia.
Lukanov
Tzvetan Hristoforov
TH
Immunology Laboratory, University Hospital, Pleven, Bulgaria. Bulgarialukanovtz@abv.bg
Bojinova
Svetla Ivanova
SI
Popova
Vanya Slavcheva
VS
Emin
Alkan Levha
AL
Veleva
Galina Lubomirova
GL
Gecheva
Svetla Penkova
SP
Konova
Emiliana Ilieva
EI
D000328:Adult; D001792:Blood Platelets / Q000737:chemistry*; D019013:CD40 Antigens / Q000097:blood*; D023201:CD40 Ligand / Q000097:blood*; D016022:Case-Control Studies; D002449:Cell Aggregation; D005260:Female; D005434:Flow Cytometry; D017359:HELLP Syndrome / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000209:etiology; D009000:Monocytes / Q000737:chemistry*; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D011225:Pre-Eclampsia / Q000097:blood* / Q000503:physiopathology; D011247:Pregnancy / Q000097:blood*; D011248:Pregnancy Complications / Q000209:etiology; D019851:Thrombophilia / Q000209:etiology; D055815:Young Adult
D019013:CD40 Antigens; D019007:P-Selectin; D023201:CD40 Ligand