title: Sequence-specific HCV RNA quantification using the size-dependent nonlinear optical properties of gold nanoparticles.
abstract: The hepatitis C virus (HCV) is a single-stranded (ss) RNA virus that is responsible for chronic liver diseases, such as cirrhosis, end-stage liver disease, and hepatocellular carcinoma. Driven by the need to detect the presence of the HCV viral sequence, herein it is demonstrated for the first time that the nonlinear optical (NLO) properties of gold nanoparticles can be used for screening and quantifying HCV RNA without any modification, with excellent detection limit (80 pM) and selectivity (single base-pair mismatch). The hyper-Rayleigh scattering (HRS) intensity increases 25 times when label-free, 145-mer, HCV ss-RNA is hybridized with 400 pM target RNA. The mechanism of HRS intensity change is discussed with experimental evidence for a higher multipolar contribution to the NLO response of gold nanoparticles.
authors.affiliation: Department of Chemistry, Jackson State University, Jackson, MS 39217, USA.
authors.forename: Jelani
authors.initials: J
authors.lastname: Griffin
authors.forename: Anant Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Dulal
authors.initials: D
authors.lastname: Senapati
authors.forename: Eumin
authors.initials: E
authors.lastname: Lee
authors.forename: Kevin
authors.initials: K
authors.lastname: Gaylor
authors.forename: Jeanette
authors.initials: J
authors.lastname: Jones-Boone
authors.forename: Paresh Chandra
authors.initials: PC
authors.lastname: Ray
mesh_terms: D006046:Gold; D016174:Hepacivirus / Q000737:chemistry*; D053768:Metal Nanoparticles; D017711:Nonlinear Dynamics; D009693:Nucleic Acid Hybridization; D055620:Optical Phenomena; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral; D006046:Gold