title: [Phenol degradation by Rhodococcus opacus strain 1G].
abstract: During cultivation in a liquid medium, the bacterium Rhodococcus opacus 1G was capable of growing on phenol at a concentration of up to 0.75 g/l. Immobilization of Rhodococcus opacus 1G had a positive effect on cell growth in the presence of phenol at high concentrations. The substrate at concentrations of 1.0 and 1.5 g/l was completely utilized over 24 and 48 h, respectively. The key enzymes of phenol degradation (two pyrocatechol 1,2-dioxygenases and muconate cycloisomerase) were isolated. One of the dioxygenases was very unstable. By substrate specificity, another enzyme belonged to pyrocatechol 1,2-dioxygenases of the classical ortho-pathway. Chloropyrocatechols and chlorophenols served as competitive antagonists of pyrocatechol 1,2-dioxygenases. The inhibitory effect of other aromatic compounds was less significant. Our results suggest that this strain holds promise for bioremediation of phenol wastewater.
authors.forename: E S
authors.initials: ES
authors.lastname: Shumkova
authors.forename: I P
authors.initials: IP
authors.lastname: Solianikova
authors.forename: E G
authors.initials: EG
authors.lastname: Plotnikova
authors.forename: L A
authors.initials: LA
authors.lastname: Golovleva
mesh_terms: D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase / Q000378:metabolism*; D002733:Chlorophenols / Q000737:chemistry; D019753:Intramolecular Lyases / Q000378:metabolism*; D019800:Phenol / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology*
chemical_list: D002733:Chlorophenols; D019800:Phenol; D050548:Catechol 1,2-Dioxygenase; D019753:Intramolecular Lyases; C021811:muconate cycloisomerase