title: Ion channels underlying stimulus-exocytosis coupling and its cell-to-cell heterogeneity in beta-cells of transplantable porcine islets of Langerhans.
abstract: Given the growing interest in porcine islets as model tissue for studying the pathogenesis of human diabetes mellitus and its treatment by transplantation, we investigated stimulus-exocytosis coupling in single porcine beta-cells using patch clamp electrophysiology, Ca2+ imaging, capacitance tracking and amperometry. We establish that porcine beta-cells display several features prominently seen in beta-cells from human islets of Langerhans. These include: (i) wide heterogeneity of electrical responsiveness to glucose; (ii) dependence of action potential activity on voltage-dependent Na(+) as well as high voltage activated Ca2+ current; (iii) heterogeneity of time course of depolarization-evoked insulin granule exocytosis; and (iv) the dependence of vigorous single cell electrical activity and insulin granule exocytosis on the presence of agents that enhance cytosolic cAMP concentration. These findings promote the usefulness of porcine beta-cells as a model for studying beta-cell function in large mammals, including humans, as well as an appropriate source of tissue for xenotransplantation.
authors.affiliation: Departments of Internal Medicine and Cell Biology/Physiology, Washington University Medical Center, Saint Louis, MO 63110, USA.
authors.forename: Amelia M
authors.initials: AM
authors.lastname: Silva
authors.forename: Adam S
authors.initials: AS
authors.lastname: Dickey
authors.forename: David W
authors.initials: DW
authors.lastname: Barnett
authors.forename: Stanley
authors.initials: S
authors.lastname: Misler
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004594:Electrophysiology; D005089:Exocytosis; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D050417:Insulin-Secreting Cells / Q000502:physiology*; D007473:Ion Channels / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology; D016381:Islets of Langerhans Transplantation; D023421:Models, Animal; D012964:Sodium / Q000378:metabolism; D013552:Swine
chemical_list: D007473:Ion Channels; D012964:Sodium; D005947:Glucose; D002118:Calcium