Separation of protein oligomers by blue native gel electrophoresis.
Native gel electrophoresis is used as a tool to assess structural differences in proteins. This article presents an application to separate oligomeric forms of proteins such as human immunodeficiency virus type 1 (HIV-1) reverse transcriptase monomers and homodimers. Technical difficulties encountered with various native gel techniques and ways to circumvent them are described.
Braz
Valerie A
VA
Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA. vab6@case.edu
Howard
Kathryn J
KJ
D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D054303:HIV Reverse Transcriptase / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry
D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; C004692:coomassie Brilliant Blue