Ontogeny and phagocytic function of baboon lung dendritic cells.
Dendritic cells (DCs) are the most potent antigen-presenting cells, but the ontogeny and functions of lung DCs are not known during prenatal period. Here, we isolated lung DC population from fetal (125-175 days of gestation age) and adult baboons. The cells were stained with fluorochrome-conjugated-HLA-DP, DQ, DR, CD1a, CD11c, CD14, CD40, CD80, CD86, CD209, CMKLR1, ILT7-specific antibodies, and staining was analyzed by flow cytometry. The phagocytic function was investigated by incubating the cells with fluorescent-labeled Escherichia coli bioparticles and analyzed by flow cytometry and fluorescence microscopy. The fetal baboon lung DCs expressed low levels of HLA-DP, DQ, DR, CD11c and CD86 as compared to adult baboon lung DCs and showed distinct DC morphology. The fetal lung DCs were also less capable of phagocytosing E. coli as compared to the adult lung DCs (P<0.05). In conclusion, the fetal lung DCs are not only phenotypically immature, but also less efficient in phagocytosing E. coli.
Awasthi
Shanjana
S
Department of Pharmaceutical Sciences, University of Oklahoma Health Science Center, Oklahoma City, OK, USA. Shanjana-Awasthi@ouhsc.edu
Wolf
Roman
R
White
Gary
G
D000367:Age Factors; D000818:Animals; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D039521:CD11c Antigen / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D005434:Flow Cytometry; D006358:Hot Temperature; D016130:Immunophenotyping; D008168:Lung / Q000196:embryology / Q000276:immunology*; D048530:Papio anubis / Q000196:embryology / Q000276:immunology*; D048548:Papio cynocephalus / Q000196:embryology / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D039521:CD11c Antigen; D019013:CD40 Antigens