[Structrue identification of biflavones and determination of Taxol from Taxus Madia].
OBJECTIVE
To study the flavonoids from Taxus Madia and determine the content of Taxol from different parts of Taxus Madia.


METHODS
Various column chromatographic techniques were applied for isolation and purification. The structrues were elucidated by physico-chemical property and spectral evidence. The analysis of Taxol was carried out on C18 (150 mm x 4.6 mm i. d. 5 microm). The mobile phase was MeOH-H2O (3:1). The flow-rate was 0.8 ml/min. The detection wave-length was 228 nm.


RESULTS
Three bifiavonyl compounds were isolated and identified as Sciadopitysin (Compound I), Ginkgetin (Compound II), 7,7",4'-Tri-O-Methylamentoflavone (Compound III). The contents of Taxol from branches and leaves, main root, fibrous root was 0.0308 mg/g, 0.02191 mg/g, 0.01983 mg/g respectively.


CONCLUSIONS
Compound I-III are obtained from Taxus Madia for the first time. The contents of Taxol is the highest in the part of branches and leaves, and then is the main root, the lowest is the fibrous root.
Liu
Xiang-Qian
XQ
Department of Pharmaceutical Engineering, Central South University, Changsha 410083, China. lxq0001cn@yahoo.com.cn
Zhang
Xiao-Dan
XD
Zhu
Yu-Lin
YL
Shin
Bum-Young
BY
Wu
Shi-Xu
SX
D044946:Biflavonoids / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D017239:Paclitaxel / Q000032:analysis* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D018547:Plant Stems / Q000737:chemistry / Q000254:growth & development; D020946:Taxus / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D014197:Trees / Q000737:chemistry
D044946:Biflavonoids; C077458:ginkgetin; C086243:sciadopitysin; D017239:Paclitaxel