Effect of anti-GM2 antibodies on rat sciatic nerve: electrophysiological and morphological study.
We found that a monoclonal human IgM anti-GM2 was fixed in rat sciatic axons and Schwann cells and was able to activate human complement. The passive transfer of IgM and complement in sciatic nerves can induce an acute alteration in nerve conduction. When the transfer of IgM plus complement was repeated for 10 days, the compound action motor potential amplitude was very low and the morphological study showed axons and myelin damage. Without human complement, IgM can only slightly disorganize the myelin by separating some layers, probably by interfering with the functional role of gangliosides in the myelin package.
Ortiz
Nicolau
N
Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain. nicolauantoni.ortiz@urv.net
Sabaté
M Mar
MM
Garcia
Neus
N
Santafe
Manel M
MM
Lanuza
M Angel
MA
Tomàs
Marta
M
Tomàs
Josep
J
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology; D001323:Autoantibodies / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D002908:Chronic Disease; D003711:Demyelinating Diseases / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D005678:G(M2) Ganglioside / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology; D008297:Male; D015213:Neuroimmunomodulation / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007075:Immunoglobulin M; D005678:G(M2) Ganglioside