[Expression of Aurora-B in human glioma tissue and its significance].
OBJECTIVE
To study the expression of Aurora-B in human glioma tissue and its significance.


METHODS
The total RNA was extracted from 41 human glioma tissues and 11 normal brain tissues by Trizol reagent. After reverse transcription of the total RNA into cDNAs, Aurora-B mRNA expressions in these samples were detected by quantitative real-time PCR. The protein expression in these samples was detected using immunohistochemical staining.


RESULTS
Aurora-B mRNA and protein expressions were significantly increased in glioma tissues as compared with those in normal brain tissues.


CONCLUSIONS
Aurora-B mRNA and protein show markedly higher expressions in glioma tissue, suggesting that Aurora-B may be one of the malignant biomarkers in the pathogenesis and progression of human glioma.
Sun
Li-bo
LB
Department of Neurosurgery, First Affiliated Hospital of Jilin University, Changchun 130021, China.
Fu
Shuang-lin
SL
Luo
Yi-nan
YN
Chen
Huan
H
Li
Wen-chen
WC
Ge
Peng-fei
PF
D064107:Aurora Kinase B; D064127:Aurora Kinases; D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases