title: Extracting cancer cell line electrochemical parameters at the single cell level using a microfabricated device.
abstract: Cancer cells have distinctive electrochemical properties. This work sheds light on the system design aspects and key challenges that should be considered when experimentally analyzing and extracting the electrical characteristics of a tumor cell line. In this study, we developed a cellularbased functional microfabricated device using lithography technology. This device was used to investigate the electrochemical parameters of cultured cancer cells at the single-cell level. Using impedance spectroscopy analyses, we determined the average specific capacitance and resistance of the membrane of the cancer cell line B16-F10 to be 1.154 +/- 0.29 microF/cm(2), and 3.9 +/- 1.15 KOmega.cm(2) (mean +/- SEM, n =14 cells), respectively. The consistency of our findings via different trails manifests the legitimacy of our experimental procedure. Furthermore, the data were compared with a proposed constructed analytical-circuit model. The results of this work may greatly assist researchers in defining an optimal procedure while extracting electrical properties of cancer cells. Detecting electrical signals at the single cell level could lead to the development of novel approaches for analysis of malignant cells in human tissues and biopsies.
authors.affiliation: Massachusetts Institute of Technology, Mechatronics Research Laboratory, Cambridge, MA, USA.
authors.forename: Jassim A
authors.initials: JA
authors.lastname: Alqabandi
authors.forename: Ussama M
authors.initials: UM
authors.lastname: Abdel-Motal
authors.forename: Kamal
authors.initials: K
authors.lastname: Youcef-Toumi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane; D025802:Electric Capacitance; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008545:Melanoma / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D008904:Miniaturization; D010992:Plethysmography, Impedance / Q000295:instrumentation* / Q000379:methods