Synergistic effects of alpha-1,2-fucosyltransferase, DAF, and CD59 in suppression of xenogenic immunological responses.
BACKGROUND
Previous studies showed that alpha-1,2-fucosyltransferase (HT), decay accelerating factor (DAF), and CD59 have an inhibitory effect on the immunological rejection of xenogenic transplantation.


METHODS
To investigate their possible synergistic effects in suppression of heterogeneic transplantation, we produced transgenic mouse lines expressing human HT, DAF, and/or CD59 by the standard pronuclear injection approach. PCR and Southern blot were used to identify the transgenic founder lines. Flow cytometry confirmed the high-level expression of HT, DAF, or CD59 in the transgenic mice.


RESULTS
The deposition of IgM, C3c, or C9 in the cardiac vascular endothelial cells of the HT, HT/CD59, and/or DAF multiple positive transgenic mice was markedly decreased. The survival time and function of the hearts of the co-transgenic mice were significantly longer and higher than that of the single HT-positive transgenic mice (P < 0.05).


CONCLUSIONS
The mice co-expressing HT/DAF or HT/CD59 could resist the hyperacute rejection better than those expressing HT alone. It is feasible to use HT and C-reactive proteins co-transgenic tissues to resist hyperacute rejection and xenograft rejection.
Li
Sheng-Zhi
SZ
Tianjin Institute of Urology and Second Hospital of Tianjin Medical University, Tianjin, China.
Qu
Yan-Chun
YC
Liu
Bing-Qian
BQ
Wang
Guang-You
GY
Zhang
Yue
Y
Ma
Zhi-Fang
ZF
Ma
Teng-Xiang
TX
Qiu
Mengsheng
M
Han
Rui-Fa
RF
D000818:Animals; D000910:Antibodies, Heterophile / Q000378:metabolism*; D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism*; D018957:CD59 Antigens / Q000235:genetics / Q000378:metabolism*; D015932:Complement C3c / Q000378:metabolism; D003186:Complement C9 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000276:immunology; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control; D016027:Heart Transplantation / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000276:immunology; D014183:Transplantation, Heterologous / Q000276:immunology*
D000910:Antibodies, Heterophile; D018958:CD55 Antigens; D018957:CD59 Antigens; D003186:Complement C9; D007075:Immunoglobulin M; D015932:Complement C3c; D005647:Fucosyltransferases