In situ ligation between peptides and silica nanoparticles for making peptide microarrays on polycarbonate.
Bisphenol A polycarbonate (PC) is emerging as an interesting alternative to silicon oxide substrates for making microarrays. We show that the printing of peptide/nanoparticle mixtures allows the creation of complex peptide microarrays. Semicarbazone ligation was used for linking the peptides to the nanoparticles. The reaction occurred probably after printing due to solvent evaporation and to the in situ concentration of the reagents. Peptide microarrays were used successfully for the specific capture of purified antibodies or of antibodies from serum.
Souplet
Vianney
V
Institut de Biologie de Lille, UMR CNRS 8161, Universites de Lille 1 et 2, Institut Pasteur de Lille, 1 rue du Professeur Calmette, Lille Cedex, France.
Desmet
Rémi
R
Melnyk
Oleg
O
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011076:Polycarboxylate Cement / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D044967:Serum / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
D000906:Antibodies; D010455:Peptides; D011076:Polycarboxylate Cement; C005506:polycarbonate; D012822:Silicon Dioxide