Mini-beam collimator enables microcrystallography experiments on standard beamlines.
The high-brilliance X-ray beams from undulator sources at third-generation synchrotron facilities are excellent tools for solving crystal structures of important and challenging biological macromolecules and complexes. However, many of the most important structural targets yield crystals that are too small or too inhomogeneous for a ;standard' beam from an undulator source, approximately 25-50 microm (FWHM) in the vertical and 50-100 microm in the horizontal direction. Although many synchrotron facilities have microfocus beamlines for other applications, this capability for macromolecular crystallography was pioneered at ID-13 of the ESRF. The National Institute of General Medical Sciences and National Cancer Institute Collaborative Access Team (GM/CA-CAT) dual canted undulator beamlines at the APS deliver high-intensity focused beams with a minimum focal size of 20 microm x 65 microm at the sample position. To meet growing user demand for beams to study samples of 10 microm or less, a ;mini-beam' apparatus was developed that conditions the focused beam to either 5 microm or 10 microm (FWHM) diameter with high intensity. The mini-beam has a symmetric Gaussian shape in both the horizontal and vertical directions, and reduces the vertical divergence of the focused beam by 25%. Significant reduction in background was achieved by implementation of both forward- and back-scatter guards. A unique triple-collimator apparatus, which has been in routine use on both undulator beamlines since February 2008, allows users to rapidly interchange the focused beam and conditioned mini-beams of two sizes with a single mouse click. The device and the beam are stable over many hours of routine operation. The rapid-exchange capability has greatly facilitated sample screening and resulted in several structures that could not have been obtained with the larger focused beam.
Fischetti
Robert F
RF
GM/CA CAT at the APS, Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA. rfischetti@anl.gov
Xu
Shenglan
S
Yoder
Derek W
DW
Becker
Michael
M
Nagarajan
Venugopalan
V
Sanishvili
Ruslan
R
Hilgart
Mark C
MC
Stepanov
Sergey
S
Makarov
Oleg
O
Smith
Janet L
JL
D018360:Crystallography, X-Ray / Q000295:instrumentation*; D004307:Dose-Response Relationship, Radiation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D011487:Protein Conformation / Q000528:radiation effects; D011829:Radiation Dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D017356:Synchrotrons / Q000295:instrumentation*
D046912:Multiprotein Complexes; D012996:Solutions