title: Control of Arabidopsis meristem development by thioredoxin-dependent regulation of intercellular transport.
abstract: Cell-to-cell transport in plants occurs through cytoplasmic channels called "plasmodesmata" and is regulated by developmental and environmental factors. Callose deposition modulates plasmodesmal transport in vivo, but little is known about the mechanisms that regulate this process. Here we report a genetic approach to identify mutants affecting plasmodesmal transport. We isolated 5 mutants, named gfp arrested trafficking (gat), affected in GFP unloading from the phloem into the meristem. gat1 mutants were seedling lethal and carried lesions in an m-type thioredoxin that is expressed in non-green plastids of meristems and organ primordia. Callose and hydrogen peroxide accumulated in gat1 mutants, and WT plants subjected to oxidative conditions phenocopied the gat1 trafficking defects. Ectopic expression of GAT1 in mature leaves increased plasmodesmal permeability and led to a delay in senescence and flowering time. We propose a role for the GAT1 thioredoxin in the redox regulation of callose deposition and symplastic permeability that is essential for meristem maintenance in Arabidopsis.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: Yoselin
authors.initials: Y
authors.lastname: Benitez-Alfonso
authors.forename: Michelle
authors.initials: M
authors.lastname: Cilia
authors.forename: Adrianna
authors.initials: A
authors.lastname: San Roman
authors.forename: Carole
authors.initials: C
authors.lastname: Thomas
authors.forename: Andy
authors.initials: A
authors.lastname: Maule
authors.forename: Stephen
authors.initials: S
authors.lastname: Hearn
authors.forename: David
authors.initials: D
authors.lastname: Jackson
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D010641:Phenotype; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C510128:GAT1 protein, Arabidopsis; D026901:Membrane Transport Proteins; D013879:Thioredoxins