title: [Binding activity of polypeptide containing human Na+, K+-ATPase alpha1 subunit M1-M2 extracellular segment].
abstract: OBJECTIVE
  To assess the binding activity of polypeptide containing human Na+, K+-ATPase alpha1 subunit M1-M2 extracellular segment (HES1 derivative).
  
  
  METHODS
  HES1 derivative was synthesized by Fmoc method and purified by high-performance liquid chromatography-mass spectrometry, and its binding activity was identified by radioligand binding assay.
  
  
  RESULTS
  3H-ouabain and synthetic HES1 derivative showed some binding activity with the equilibrium dissociation constant (KD) of 24.58 nmol/L, with the the receptor density of 492.43 fmol x mg(-1) pro. and IC50 of 3.078 x 10(-7) mol/L.
  
  
  CONCLUSIONS
  HES1 derivative can bind to ouabain and has the potential of becoming an effective therapeutic agent.
authors.affiliation: Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. zhangmingjuanyj@yahoo.com
authors.forename: Ming-juan
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Can-zhan
authors.initials: CZ
authors.lastname: Zhu
authors.forename: Zong-ming
authors.initials: ZM
authors.lastname: Duan
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Niu
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D010042:Ouabain / Q000737:chemistry* / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D010455:Peptides; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase