Tolerance of whitefish embryos to Pseudomonas fluorescens linked to genetic and maternal effects, and reduced by previous exposure.
Juvenile or adult fish can alter their behaviour and rely on an innate and adaptive immune system to avoid/counteract pathogens, while fish embryos have to depend on egg characteristics and may be partly protected by their developing immune system that is building up from a certain age on. We developed an infection protocol that allows testing the reaction of individual whitefish embryos (Coregonus palaea) to repeated exposures to Pseudomonas fluorescens, an opportunistic bacterial fish pathogen. We used a full-factorial in vitro breeding design to separately test the effects of paternal and maternal contributions to the embryos' susceptibility to different kinds of pathogen exposure. We found that a first non-lethal exposure had immunosuppressive effects: pre-exposed embryos were more susceptible to future challenges with the same pathogen. At intermediate and high levels of pathogen intensity, maternal effects turned out to be crucial for the embryos' tolerance to infection. Paternal (i.e. genetic) effects played a significant role at the strongest level of infection, i.e. the embryos' own genetics already explained some of the variation in embryo susceptibility. Our findings suggest that whitefish embryos are largely protected by maternally transmitted substances, but build up some own innate immunocompetence several days before hatching.
von Siebenthal
Beat A
BA
Department of Ecology and Evolution, Biophore, University of Lausanne, 1015 Lausanne, Switzerland. beat.vonsiebenthal@unil.ch
Jacob
Alain
A
Wedekind
Claus
C
D000818:Animals; D001947:Breeding; D004625:Embryo, Nonmammalian / Q000382:microbiology; D005393:Fish Diseases / Q000235:genetics* / Q000382:microbiology* / Q000401:mortality; D014644:Genetic Variation; D011552:Pseudomonas Infections / Q000235:genetics / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D011551:Pseudomonas fluorescens / Q000502:physiology*; D012487:Salmonidae / Q000235:genetics* / Q000382:microbiology*