Evaluation of mitochondrial respiratory chain activity in muscle healing by low-level laser therapy.
BACKGROUND
Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process.


OBJECTIVE
In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after traumatic muscular injury.


METHODS
Male Wistar rats were randomly divided into three groups (n=6): sham (uninjured muscle), muscle injury without treatment, muscle injury with LLLT (AsGa) 5J/cm(2). Gastrocnemius injury was induced by a single blunt-impact trauma. LLLT was used 2, 12, 24, 48, 72, 96, and 120 hours after muscle-trauma.


RESULTS
Our results showed that the activities of complex II and succinate dehydrogenase after 5days of muscular lesion were significantly increased when compared to the control group. Moreover, our results showed that LLLT significantly increased the activities of complexes I, II, III, IV and succinate dehydrogenase, when compared to the group of injured muscle without treatment.


CONCLUSIONS
These results suggest that the treatment with low-level laser may induce an increase in ATP synthesis, and that this may accelerate the muscle healing process.
Silveira
Paulo C L
PC
Laboratório de Fisiologia e Bioquímica do Exercício, Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil. silveira_paulo2004@yahoo.com.br
Silva
Luciano Acordi da
LA
Fraga
Daiane B
DB
Freitas
Tiago P
TP
Streck
Emilio L
EL
Pinho
Ricardo
R
D000818:Animals; D004579:Electron Transport / Q000502:physiology / Q000528:radiation effects; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D042963:Electron Transport Complex II / Q000528:radiation effects; D003576:Electron Transport Complex IV / Q000528:radiation effects; D028022:Low-Level Light Therapy; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism / Q000528:radiation effects*; D018482:Muscle, Skeletal / Q000293:injuries*; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism / Q000528:radiation effects; D014945:Wound Healing / Q000502:physiology* / Q000528:radiation effects
D045222:Electron Transport Chain Complex Proteins; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV