The effect of rhG-CSF on the conformation of LFA-1 on CD4+ T cells in hemopoietic stem cell transplantation.
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) modulates donor T cell function in hemopoietic stem cell transplantation. The effects of rhG-CSF on the activation of CD4(+) T cells have been poorly investigated. We investigated whether rhG-CSF mobilization influenced the activation and proliferation capacity of CD4(+) T cells. Cell treatment with phorbol 12-myristate 13-acetate (PMA) plus ionomycin or the CD3 mAb OKT3 plus intercellular cell adhesion molecule-1 (ICAM-1) at 37 degrees C for 6 h induced a dramatic increase in CD25, CD69 and MEM148 epitope exposure. rhG-CSF mobilization decreased CD25, CD69 and MEM148 epitope expression on activated CD4(+) T cells compared with cells before mobilization. The transcription factor Jun activation domain-binding protein 1 (JAB1) plays a role in the activation of CD4(+) T cells, and the rhG-CSF mobilization changed the level of nuclear JAB1 protein. rhG-CSF mobilization also decreased the adhesion of CD4(+) T cells to ICAM-1, but had no effect on the levels of donor CD4+CD25+ regulatory T cells. Overall, these data suggest the rhG-CSF mobilization can influence CD4(+) T cell activation through LFA-1/ICAM-1 costimulatory signaling in HSC transplantation.
Weihua
Chen
C
Department of Hematology, PLA General Hospital, Beijing, China.
Fei
Wang
W
Meng
Li
L
Haiyan
Zhu
Z
Wanming
Da
D
Chunji
Gao
G
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D000075686:COP9 Signalosome Complex; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology; D015759:Ionomycin / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000276:immunology; D011487:Protein Conformation / Q000187:drug effects; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D055815:Young Adult; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000075686:COP9 Signalosome Complex; D016179:Granulocyte Colony-Stimulating Factor; D018799:Intercellular Adhesion Molecule-1; D053645:Interleukin-2 Receptor alpha Subunit; D047908:Intracellular Signaling Peptides and Proteins; D015759:Ionomycin; D037181:Lectins, C-Type; D016169:Lymphocyte Function-Associated Antigen-1; D010447:Peptide Hydrolases; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate; D000099192:CD69 Antigens; C501488:COPS5 protein, human