title: Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members.
abstract: The nucleotide sequence of Pineapple mealybug wilt associated virus-3 (PMWaV-3) (Closteroviridae: Ampelovirus), spanning seven open reading frames (ORFs) and the untranslatable region of the 3' end was determined. Based on the amino acid identities with orthologous ORFs of PMWaV-1 (54%-73%) and PMWaV-2 (13%-35%), we propose PMWaV-3 is a new species in the PMWaV complex. PMWaV-3 lacks an intergenic region between ORF1b and ORF2, encodes a relatively small, 28.8 kDa, coat protein, and lacks a coat protein duplicate. Phylogenetic analyses were used to analyze seven different domains and ORFs from members of the family Closteroviridae. Two distinct clades within the recognized genus Ampelovirus were observed; one that includes PMWaV-3 and PMWaV-1 and several GLRaVs and another that includes PMWaV-2 and GLRaV-3, the type member of the genus Ampelovirus.
authors.affiliation: Plant and Environmental Protection Sciences, University of Hawaii at Manoa, 3190 Maile Way, Honolulu, HI 96822, USA.
authors.forename: Diane M
authors.initials: DM
authors.lastname: Sether
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Melzer
authors.forename: Wayne B
authors.initials: WB
authors.lastname: Borth
authors.forename: John S
authors.initials: JS
authors.lastname: Hu
mesh_terms: D020413:3' Untranslated Regions; D031230:Ananas / Q000821:virology; D029381:Closteroviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D023061:Gene Order; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins