title: Prevalence of antibody to hepatitis E virus among pet dogs in the Jiang-Zhe area of China.
abstract: In order to investigate the prevalence of HEV infection and immunity in pet dogs in the Jiang-Zhe area of China, we examined 192 dog serum samples collected from 11 animal hospitals in this area for anti-HEV IgG and HEV RNA, using ELISA and RT-PCR assays, respectively. Two dogs were injected intravenously with swine HEV to obtain anti-HEV antibody positive serum for ELISA assay. After the ELISA assay, 26 samples showing OD values higher than 0.20 were selected and examined by Dot-blot assay, and 88.5% (23/26) were confirmed to be positive for anti-HEV IgG. Although our results indicated swine HEV could experimentally induce anti-HEV IgG in the serum, we failed to detect HEV RNA in the serum samples from either the 2 intravenously injected dogs or 192 serum samples collected from animal hospitals. This implied that dog may not be a reservoir of HEV in this area.
authors.affiliation: Group of Zoonosis and Comparative Medicine, School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, PR China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.forename: Shixing
authors.initials: S
authors.lastname: Yang
authors.forename: Fen
authors.initials: F
authors.lastname: Huang
authors.forename: Peifeng
authors.initials: P
authors.lastname: Li
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Guo
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
mesh_terms: D000818:Animals; D002681:China; D004283:Dog Diseases / Q000453:epidemiology* / Q000276:immunology* / Q000821:virology; D004285:Dogs; D006508:Hepatitis Antibodies / Q000097:blood*; D016751:Hepatitis E / Q000453:epidemiology / Q000276:immunology* / Q000662:veterinary*; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006762:Hospitals, Animal; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood; D016036:Seroepidemiologic Studies
chemical_list: D006508:Hepatitis Antibodies; D012367:RNA, Viral