Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane.
We report our experience with Gnathostoma protein preparation by the ultrafiltration method. Crude antigen was sonicated and ultrafiltrated using the Nanosep 100 K membrane. SDS-PAGE electrophoresis showed protein bands at 43, 41, 24, 22, 21, 19.5 kDa. Use of the ultrafiltration method can provide specific protein (24 kDa), similar to the non-ultrafiltration method, with the other 5 non-specific proteins. Using the non-ultrafiltration method, there were more (20) non-specific protein. The ultrafiltration method can be an alternative method for the preparation of protein, which can provide better results than non-ultrafiltration.
Sugaroon
Suphan
S
Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Saksirisampant
Wilai
W
Kraivichian
Kanyarattana
K
Suwansaksri
Jamsai
J
Wiwanitkit
Viroj
V
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D045424:Complex Mixtures / Q000737:chemistry; D006039:Gnathostoma / Q000276:immunology*; D015801:Helminth Proteins / Q000302:isolation & purification*; D008567:Membranes, Artificial; D036103:Nanotechnology; D014462:Ultrafiltration
D000947:Antigens, Helminth; D045424:Complex Mixtures; D015801:Helminth Proteins; D008567:Membranes, Artificial