Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis.
Selenium (Se) plays a critical role in testis, sperm, and reproduction, and testis Se levels are remarkably maintained in Se deficiency. In most other tissues, Se levels decrease dramatically as do levels of most selenoproteins and levels of a subset of Se-regulated selenoprotein mRNAs. Because of the recent identification of key molecules in the targeted trafficking of Se to the testis, we examined the hierarchy of Se regulation in testis by determining the dietary Se regulation of the full testis selenoproteome in rats fed graded levels of Se (0 to 0.8 microg Se/g) as Na2SeO3 for 28 d. Se status did not significantly affect testis weight or glutathione peroxidase 4 (Gpx4) activity (P>0.05). qRT-PCR analysis of selenoprotein mRNA expression revealed that 21 of the 24 selenoprotein mRNAs and ApoER2 mRNA (the selenoprotein P [Sepp1] receptor) were also not regulated significantly by dietary Se status. In contrast, Gpx1 activity decreased to 28% of Se-adequate levels, and mRNA levels for Gpx1, Sepp1, and Sepw1 (selenoprotein W) decreased significantly in Se-deficient rats to 45, 46, and 55%, respectively, of Se-adequate plateau levels. Overlap of hyperbolic Gpx4 activity and Sepw1 mRNA response curves with testis Se concentration, all with minimum dietary Se requirements<0.016 microg Se/g, showed the priority for synthesis of Gpx4. Higher minimum dietary Se requirements of 0.04 microg Se/g for Gpx1 activity and Sepp1 mRNA, and the even higher minimum dietary Se requirement of 0.08 microg Se/g for Gpx1 mRNA, suggest that the hierarchy of these biomarkers reflects distinct, lower priority pools, cell types, and roles for Se within the testis.
Schriever
Sonja C
SC
Department of Nutritional Sciences, University of Wisconsin, 1415 Linden Drive, Madison, WI 53706, USA. Sonja_Charlotte.Schriever@med.uni-muenchen.de
Barnes
Kimberly M
KM
Evenson
Jacqueline K
JK
Raines
Anna M
AM
Sunde
Roger A
RA
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D019587:Dietary Supplements; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D026502:LDL-Receptor Related Proteins; D008297:Male; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D018110:Receptors, Lipoprotein / Q000096:biosynthesis; D012098:Reproduction / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012643:Selenium / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology; D051151:Selenoprotein W / Q000096:biosynthesis; D013094:Spermatozoa / Q000201:enzymology; D013737:Testis / Q000201:enzymology*; D000094765:Glutathione Peroxidase GPX1
D026502:LDL-Receptor Related Proteins; D012333:RNA, Messenger; D018110:Receptors, Lipoprotein; D051151:Selenoprotein W; C098773:low density lipoprotein receptor-related protein 8; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; C523860:glutathione peroxidase 4, rat; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat