Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS.
Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and beta-galactosidase respectively when using SERRS.
Ingram
Andrew
A
WestChem, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK.
Moore
Barry D
BD
Graham
Duncan
D
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002625:Chemistry, Organic / Q000379:methods; D004396:Coloring Agents / Q000494:pharmacology; D004952:Esters / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D015394:Molecular Structure; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D001616:beta-Galactosidase / Q000032:analysis* / Q000378:metabolism
D004396:Coloring Agents; D004952:Esters; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase