title: Medium-chain fatty acids ameliorate insulin resistance caused by high-fat diets in rats.
abstract: BACKGROUND
  High dietary intake of saturated fat impairs insulin sensitivity and lipid metabolism. The influence of fatty acid chain length, however, is not yet fully understood, but evidence exists for different effects of saturated long-chain (LC) versus saturated medium-chain (MC) fatty acids (FA).
  
  
  METHODS
  To investigate the effects of the FA chain length, male Wistar rats were fed high-fat diets containing triacylglycerols composed of either MC- or LCFA for 4 weeks; rats fed maintenance diet served as a control. The animals underwent euglycemic hyperinsulinemic clamping or oral metabolic tolerance testing respectively; enzyme activities of mitochondrial (EC2.3.1.21 carnitine palmitoyl transferase) and peroxisomal (EC1.3.3.6 acyl-CoA oxidase) FA oxidation were measured in liver and muscle.
  
  
  RESULTS
  LCFA consumption resulted in higher fasted serum insulin and glucose concentrations compared to controls, while MCFA-fed animals did not differ from controls. Insulin sensitivity was reduced by 30% in the LCFA group while the MCFA group did not differ from controls. Feeding MCFA resulted in the controls' lowered fasted and post-prandial triacylglycerol concentration compared to LCFA, while triacylglycerol concentrations in muscle were higher in both high-fat groups compared to controls. No diet-induced changes were found in acyl-CoA oxidase (ACO) activity (liver and muscle), while LCFA feeding significantly raised carnitine palmitoyltransferase activity.
  
  
  CONCLUSIONS
  The chain length of saturated fatty acids in isocaloric diets affects insulin sensitivity, lipid metabolism and mitochondrial fatty acid oxidation without influencing body weight. While dietary LCFA clearly impair insulin sensitivity and lipid metabolism, MCFA seem to protect from lipotoxicity and subsequent insulin resistance without caloric restriction.
authors.affiliation: Diabetes and Nutrition Research Laboratory, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. wein@aninut.uni-kiel.de
authors.forename: Silvia
authors.initials: S
authors.lastname: Wein
authors.forename: Siegfried
authors.initials: S
authors.lastname: Wolffram
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schrezenmeir
authors.forename: Daniela
authors.initials: D
authors.lastname: Gasperiková
authors.forename: Iwar
authors.initials: I
authors.lastname: Klimes
authors.forename: Elena
authors.initials: E
authors.lastname: Seböková
mesh_terms: D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D004032:Diet; D004041:Dietary Fats; D004734:Energy Metabolism; D005227:Fatty Acids / Q000627:therapeutic use*; D005230:Fatty Acids, Nonesterified / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D024821:Metabolic Syndrome / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D007328:Insulin