[Effect of a simple morphine system injection in some aminoacids in the anterior cingulate cortex during acute pain].
The aim of this research was to find out the effects of ip morphine pretreatment in the extracellular content of the arginine, glutamate, aspartate and GABA levels in the anterior cingulate cortex in rats, during the formalin test (phase I). A combination of micro dialysis and Capillary Electrophoresis Zone and laser-induced fluorescence detection (CZE-LIFD) technique was used to measure the extracellular levels of amino acids in microdialized zones. The microdialysis probes were unilaterally implanted in the left anterior cingulate cortex of freely moving rats. The samples were collected every 30 seconds and derivatized with fluorescein isothiocianate. The arginine, glutamate, aspartate and GABA levels were measured in the CZE-LIFD device. Arginine (p<0.001) and glutamate levels (p<0.012) were significantly increased in the first few minutes following the formalin test (phase 1). Pretreatment with morphine suppressed the glutamate increase. A transient GABA level increase (p<0.001) was also detected. These experiments suggest that rapid changes in neurotransmitters levels were detected in the first few minutes of acute pain as revealed by the glutamate and arginine level increases in the anterior cingulate cortex. These changes could be related to the emotion of pain processing (fear and aversion). Morphine pretreatment produced an increase in GABA levels and a decrease in glutamate levels in the first few minutes. These findings may be related to euphoria and/or analgesia.
Silva
Elizabeth
E
Laboratorio de Fisiología de la Conducta, Departamento de Fisiología, Escuela de Medicina, Universidad de los Andes, Mérida, Venezuela. rosas@ula.ve
Quiñones
Belkis
B
Páez
Ximena
X
Hernández
Luis
L
D000208:Acute Disease; D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000032:analysis / Q000378:metabolism; D001224:Aspartic Acid / Q000032:analysis / Q000378:metabolism; D002540:Cerebral Cortex / Q000737:chemistry; D004202:Disinfectants / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism; D006179:Gyrus Cinguli / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D017551:Microdialysis; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology*; D010146:Pain / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000032:analysis / Q000378:metabolism
D000596:Amino Acids; D000701:Analgesics, Opioid; D004202:Disinfectants; D005557:Formaldehyde; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D009020:Morphine; D001120:Arginine