title: Subsets of retinal neurons and glia express P2Y1 receptors.
abstract: Recent evidence suggests that extracellular ATP modulates retinal processing and could play a role in modulating glial cells during retinal diseases. Here, we evaluated the localization of P2Y(1) receptors in the rat retina using indirect immunofluorescence immunocytochemistry. We observed labeling within defined populations of inner retinal neurons and Müller cell processes and end feet. Double labeling of P2Y(1) receptor with choline acetyltransferase revealed extensive colocalization indicating the expression of this receptor by cholinergic amacrine cells. Ganglion cell labeling for P2Y(1) receptors was also observed. Having established the normal pattern of immunolabeling within the retina, we next examined whether immunolabeling was altered by retinal disease. P2Y(1) receptor immunolabeling of Müller cells was of greater intensity following light-induced retinal degeneration, suggesting that Müller cell gliosis is accompanied by changes in P2Y(1) receptor expression. Overall, these data provide further evidence for a role of extracellular ATP in retinal signaling within subsets of retinal neurons as well as glia.
authors.affiliation: Department of Anatomy and Cell Biology, The University of Melbourne, Grattan Street, Parkville 3010, Victoria, Australia.
authors.forename: M M
authors.initials: MM
authors.lastname: Ward
authors.forename: E L
authors.initials: EL
authors.lastname: Fletcher
mesh_terms: D025042:Amacrine Cells / Q000378:metabolism; D000818:Animals; D002799:Cholinergic Fibers / Q000378:metabolism; D005911:Gliosis / Q000378:metabolism* / Q000473:pathology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058472:Receptors, Purinergic P2Y1; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D012162:Retinal Degeneration / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology; D014018:Tissue Distribution
chemical_list: C546384:P2ry1 protein, rat; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1