title: Molecular characterization of early colonizer bacteria from wastes in a steel plant.
abstract: OBJECTIVE
  Forty-nine bacteria isolated from four newly-produced waste samples of a steel industry, which had a high content of CaO, MgO, Cr and P2O5, were characterized molecularly and phenotypically by susceptibility testing against heavy metals.
  
  
  RESULTS
  Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to nine genera, Pseudomonas, Micrococcus, Acinetobacter, Bacillus, Dietzia, Kocuria, Diaphorobacter, Staphylococcus and Brevibacillus. Besides, some isolates could be affiliated to species: M. luteus, Ac. junii, Ac. schindleri, B. cereus, K. marina, D. nitroreducens and Staph. warneri. The bacteria that were characterized are taxonomically diverse, and Pseudomonas and Micrococcus predominated. Fingerprinting BOX-PCR revealed high genomic heterogeneity among the isolates. Among the heavy metal compounds Zn, Ni, Pb and Cu were least toxic to the bacterial isolates, whereas Ag inhibited all isolates at 0.001 mmol l(-1).
  
  
  CONCLUSIONS
  Heterotrophic bacteria, affiliated with several phylogentic groups, were able to colonize different wastes of a steel industry.
  
  
  CONCLUSIONS
  This study extends our knowledge of the early colonizers bacteria populating siderurgic environments. Some of these bacteria could have potential for recycling siderurgic waste for steel production.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: D B
authors.initials: DB
authors.lastname: Freitas
authors.forename: C I
authors.initials: CI
authors.lastname: Lima-Bittencourt
authors.forename: M P
authors.initials: MP
authors.lastname: Reis
authors.forename: P S
authors.initials: PS
authors.lastname: Costa
authors.forename: P S
authors.initials: PS
authors.lastname: Assis
authors.forename: E
authors.initials: E
authors.lastname: Chartone-Souza
authors.forename: A M A
authors.initials: AM
authors.lastname: Nascimento
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity; D014644:Genetic Variation; D016680:Genome, Bacterial; D007220:Industrial Waste / Q000032:analysis; D019216:Metals, Heavy / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013232:Steel
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D007220:Industrial Waste; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D013232:Steel