Aptamer-based electrochemical biosensor for Botulinum neurotoxin.
We have developed an aptamer-based electrochemical sensor for detection of Botulinum neurotoxin, where steric hindrance is applied to achieve specific signal amplification via conformational change of the aptamer. The incubation time and potassium concentration of the reaction buffer were found to be key parameters affecting the sensitivity of detection of the recognition of Botulinum neurotoxin by the aptamer. Under optimized experimental conditions, a high signal-to-noise ratio was obtained within 24 h with a limit of detection (LOD) of 40 pg/ml by two standard deviation cutoffs above the noise level.
Wei
Fang
F
Department of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, University of California at Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095-1597, USA.
Ho
Chih-Ming
CM
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D019274:Botulinum Toxins, Type A / Q000032:analysis*; D050091:Dendrimers / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
D052157:Aptamers, Nucleotide; D050091:Dendrimers; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D019809:Streptavidin; D019274:Botulinum Toxins, Type A