Effect of an oil byproduct from conjugated linoleic acid (CLA) purification on CLA accumulation and lipogenic gene expression in broilers.
A previous study showed that supplementing broilers with an oil byproduct obtained during the purification process of conjugated linoleic acid (CLA) from safflower oil could result in CLA-enriched egg yolks more efficiently than feeding purified CLA (free fatty acid form). On this basis, this study evaluated whether dietary CLA byproduct (CBP) supplementation would enhance CLA accumulation in broiler muscle and its lipogenic mRNA expression in the liver. A total of 456 1-day-old male broiler chicks were randomly assigned to four groups, each of which was given one of the following 2% dietary supplements for 4 weeks: soybean oil (control), safflower oil (SAF), purified CLA, and CBP. During the feeding trial, little alteration in broiler performance was observed among the test groups. CLA accumulation efficiency in the breast muscle did not differ significantly between the CLA- and CBP-fed groups after feeding of the test diet for 3 weeks. CLA supplementation also induced lipogenesis in the livers of the broilers, and it significantly increased the relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), as well as its target genes: fatty acid synthase (FAS) and acetyl coenzyme A carboxylase (ACC) (p < 0.05). However, in the CBP-fed group, SREBP1 and ACC mRNA levels were not significantly different from the controls (p > 0.05). These results suggest that CBP could be an efficient dietary source that promotes CLA accumulation in broiler muscle without inducing lipogenesis in the liver or compromising performance and meat quality in the birds.
Kim
Jun Ho
JH
Department of Food and Biotechnology, Korea University, Chungnam, Korea.
Jeong
Wan Sik
WS
Kim
In Ho
IH
Kim
Hyo-Jin
HJ
Kim
Sang Ho
SH
Kang
Geun Ho
GH
Lee
Hong-Gu
HG
Yoon
Ho Geun
HG
Ham
Hee-Jin
HJ
Kim
Young Jun
YJ
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D019587:Dietary Supplements; D015870:Gene Expression / Q000187:drug effects*; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000302:isolation & purification / Q000378:metabolism*; D050155:Lipogenesis / Q000235:genetics*; D008099:Liver / Q000737:chemistry; D008297:Male; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D012450:Safflower Oil / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry
D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D012333:RNA, Messenger; D013024:Soybean Oil; D012450:Safflower Oil