PCR diagnosis of visceral leishmaniasis in an endemic region, Mymensingh district, Bangladesh.
Detection of Leishmania parasites in a clinical sample is necessary to confirm a suspected case of leishmaniasis. We compared the sensitivity of internal transcribed spacer 1-PCR (ITS 1-PCR) assay for parasite diagnosis with that of microscopic detection in clinical samples from kala-azar (KA) or post-kala-azar dermal leishmaniasis (PKDL) suspects in Mymensingh. Of 39 specimens collected from 35 KA and four PKDL suspects, 26 were positive by microscopic examination of smears from bone marrow and skin exudates; 38 specimens spotted on filter paper and 27 of the 28 Giemsa-stained slides tested by PCR proved positive by ITS1-PCR.
Alam
Mohammad Zahangir
MZ
Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, Berlin, Germany. mzalam12@yahoo.com
Shamsuzzaman
Abul Khair M
AK
Kuhls
Katrin
K
Schönian
Gabriele
G
D000818:Animals; D001459:Bangladesh; D001853:Bone Marrow / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000469:parasitology; D008853:Microscopy; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan; D015800:Protozoan Proteins