Specific Removal of Monocytes from Peripheral Blood of Septic Patients by Polymyxin B-immobilized Filter Column.
Lipopolysaccharide (LPS) is one of the major causes of septic shock. The polymyxin B-immobilized filter column (PMX) was developed for the adsorption of endotoxin by direct hemoperfusion and has been used for the treatment of LPS-induced septic shock. In this study, we demonstrated that PMX also specifically bound monocytes from the peripheral blood leukocytes of septic patients by mean of an analysis of bound cells using immunocytochemical and electron microscopic techniques. The specific removal of monocytes from septic patients may produce beneficial effects by reducing the interaction between monocytes and functionally associated cells including vascular endothelial cells.
Nishibori
Masahiro
M
Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. mbori@md.okayama-u.ac.jp
Takahashi
Hideo K
HK
Katayama
Hiroshi
H
Mori
Shuji
S
Saito
Shinya
S
Iwagaki
Hiromi
H
Tanaka
Noriaki
N
Morita
Kiyoshi
K
Ohtsuka
Aiji
A
D000327:Adsorption; D000368:Aged; D039481:CD11b Antigen / Q000097:blood; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005374:Filtration; D006801:Humans; D019041:L-Selectin / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D011112:Polymyxin B / Q000737:chemistry*; D018805:Sepsis / Q000097:blood / Q000628:therapy*
D039481:CD11b Antigen; C509295:ITGAM protein, human; D019041:L-Selectin; D011112:Polymyxin B