title: Establishment of an animal model of ischemic type intrahepatic biliary lesion in rabbits.
abstract: OBJECTIVE
  To explore a method to establish an animal model of ischemic type intrahepatic biliary lesion in rabbits.
  
  
  METHODS
  Forty Japanese white rabbits of clean grade were divided randomly into four groups (10 rabbits per group) including sham operation (SO) group, and artery-bile obstruction (ABO)-1 h group, ABO-2 h group and ABO-3 h group. All the rabbits in this study underwent the same initial surgical procedure in which the liver was prepared as for graft removal during liver transplantation. Subsequently in the SO group, no additional vascular intervention was performed, while in groups ABO-1 h, ABO-2 h and ABO-3 h, the animals underwent combined clamping of the hepatic artery and common bile duct with microvascular clips for 1, 2 and 3 h, respectively. After the scheduled occlusion time, the clip was removed to recover blood supply. The animals were killed 4 wk after operation. The survival rate, liver function, cholangiography and histopathological manifestation of the rabbits in each group were observed.
  
  
  RESULTS
  The survival rate was 100% in groups SO, ABO-1 h and ABO-2 h, while it was 60% in group ABO-3 h. At each observation time, the change degree of the indexes of liver function was proportional to the clamping time (ABO-3 h > ABO-2 h > ABO-1 h > SO, P < 0.05). Cholangiographical and histopathologic manifestations both showed that intrahepatic biliary lesion aggravated proportionally with the increase of the clamping time.
  
  
  CONCLUSIONS
  An animal model of ischemic type intrahepatic biliary lesion in rabbits is successfully established, which may provide a reliable technique for basic and clinical research into the etiology, development and prophylaxis of ischemic type intrahepatic biliary lesion after liver transplantation.
authors.affiliation: Department of Hepatobiliary and Pancreatosplenic Surgery, Beijing Chaoyang Hospital affiliated to the Capital Medical University, Beijing 100020, China.
authors.forename: Qin-Song
authors.initials: QS
authors.lastname: Sheng
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Chen
authors.forename: Ren
authors.initials: R
authors.lastname: Lang
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Yong-Jiu
authors.initials: YJ
authors.lastname: Yang
authors.forename: Zhao-Wei
authors.initials: ZW
authors.lastname: Qu
authors.forename: De-Fang
authors.initials: DF
authors.lastname: Zhao
authors.forename: Xiao-Sheng
authors.initials: XS
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001659:Biliary Tract / Q000000981:diagnostic imaging / Q000473:pathology; D001660:Biliary Tract Diseases / Q000000981:diagnostic imaging / Q000473:pathology*; D002758:Cholangiography; D004195:Disease Models, Animal; D007511:Ischemia / Q000000981:diagnostic imaging / Q000473:pathology*; D008111:Liver Function Tests; D011817:Rabbits