A molybdopterin oxidoreductase is involved in H2 oxidation in Desulfovibrio desulfuricans G20.
Three mutants deficient in hydrogen/formate uptake were obtained through screening of a transposon mutant library containing 5,760 mutants of Desulfovibrio desulfuricans G20. Mutations were in the genes encoding the type I tetraheme cytochrome c(3) (cycA), Fe hydrogenase (hydB), and molybdopterin oxidoreductase (mopB). Mutations did not decrease the ability of cells to produce H(2) or formate during growth. Complementation of the cycA and mopB mutants with a plasmid carrying the intact cycA and/or mopB gene and the putative promoter from the parental strain allowed the recovery of H(2) uptake ability, showing that these specific genes are involved in H(2) oxidation. The mop operon encodes a periplasm-facing transmembrane protein complex which may shuttle electrons from periplasmic cytochrome c(3) to the menaquinone pool. Electrons can then be used for sulfate reduction in the cytoplasm.
Li
Xiangzhen
X
Department of Botany and Microbiology, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Luo
Qingwei
Q
Wofford
Neil Q
NQ
Keller
Kimberly L
KL
McInerney
Michael J
MJ
Wall
Judy D
JD
Krumholz
Lee R
LR
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D045285:Desulfovibrio desulfuricans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D005816:Genetic Complementation Test; D006859:Hydrogen / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism; D008954:Models, Biological; D000090006:Molybdenum Cofactors; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D011621:Pteridines / Q000378:metabolism
D001426:Bacterial Proteins; D003067:Coenzymes; D004251:DNA Transposable Elements; D005561:Formates; D006859:Hydrogen; D008565:Membrane Proteins; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D010088:Oxidoreductases; D011621:Pteridines; C030544:formic acid; C022261:molybdopterin