Correlation between sPLA2-IIA and phosgene-induced rat acute lung injury.
Secreted phospholipase A(2) of group IIA (sPLA(2)-IIA) has been involved in a variety of inflammatory diseases, including acute lung injury. However, the specific role of sPLA(2)-IIA in phosgene-induced acute lung injury remains unidentified. The aim of the present study was to investigate the correlation between sPLA(2)-IIA activity and the severity of phosgene-induced acute lung injury. Adult male rats were randomly exposed to either normal room air (control group) or a concentration of 400 ppm phosgene (phosgene-exposed group) for there are 5 phosgene-exposed groups altogether. For the time points of 1, 3, 6, 12 and 24 h post-exposure, one phosgene-exposed group was sacrificed at each time point. The severity of acute lung injury was assessed by Pa(O2)/F(IO2) ratio, wet-to-dry lung-weight ratio, and bronchoalveolar lavage (BAL) fluid protein concentration. sPLA(2)-IIA activity in BAL fluid markedly increased between 1 h and 12 h after phosgene exposure, and reached its highest level at 6 h. Moreover, the trend of this elevation correlated well with the severity of lung injury. These results indicate that sPLA(2)-IIA probably participates in phosgene-induced acute lung injury.
Chen
Hong-li
HL
Department of Toxicology, Fourth Military Medical University, Xi'an, China.
Hai
Chun-xu
CX
Liang
Xin
X
Zhang
Xiao-di
XD
Liu
Riu
R
Qin
Xu-jun
XJ
D000818:Animals; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid; D002199:Capillary Permeability / Q000187:drug effects; D002619:Chemical Warfare Agents / Q000633:toxicity*; D054501:Group II Phospholipases A2 / Q000096:biosynthesis* / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced* / Q000201:enzymology* / Q000473:pathology; D008297:Male; D009929:Organ Size; D010100:Oxygen / Q000097:blood; D010705:Phosgene / Q000633:toxicity*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D002619:Chemical Warfare Agents; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D010705:Phosgene; D016753:Interleukin-10; D054501:Group II Phospholipases A2; D010100:Oxygen