Cytotoxicity evaluation of hydroxyapatite on human umbilical cord vein endothelial cells for mechanical heart valve prosthesis applications.
BACKGROUND
Valvular heart disease is a significant cause of morbidity and mortality worldwide. Artificial heart valves are currently the most widely accepted biomedical material. However, the material's blood compatibility is still not sufficient. Biomaterial with a good biocompatibility has not yet appeared. Our initial study was to evaluate the probable cytotoxicity of hydroxyapatite (HA) on human umbilical vein endothelial cells (HUVEC) for mechanical heart valve prosthesis applications.


RESULTS
Cell growth was tested by MTT assay and the cell relative growth rate (RGR) was calculated. Cells were cultured in media consisting of either leaching extracts of hydroxyapatite at 37 degrees C or leaching extracts at 121 degrees C or a negative and a positive control group, respectively. The cytotoxicity was graded using generally accepted standards. The RGR of cell lines cultured in the culture media consisting of either normal or heat temperature leaching extracts of hydroxyapatite and the negative controls were higher than in the positive controls. For 24 h, 48 h, and 72 h cultures, the cytotoxicity grade of HA was 0 in the normal temperature leaching extracts of hydroxyapatite and 1 in the heat temperature leaching extracts of hydroxyapatite.


CONCLUSIONS
Human umbilical cord endothelial cells grew well in the HA extracts. Our results indicate that HA possesses good IN VITRO bioactivity and biocompatibility with human umbilical cord endothelial cells and could be used as film on mechanical heart valve prostheses.
Sha
Ji-ming
JM
Cardiothoracic Surgery, Chao Hu First People's Hospital, Chao Hu, China. shajiming@163.com
Tao
Yi-qin
YQ
Yan
Zhong-ya
ZY
Li
Dao-hang
DH
Hu
Hai-feng
HF
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000737:chemistry / Q000633:toxicity*; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006350:Heart Valve Prosthesis; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D011474:Prosthesis Design; D013499:Surface Properties; D013997:Time Factors; D014471:Umbilical Veins / Q000187:drug effects* / Q000473:pathology
D020099:Coated Materials, Biocompatible; D017886:Durapatite