title: Improvement of liquid and frozen-thawed semen quality of Nili-Ravi buffalo bulls (Bubalus bubalis) through supplementation of fat.
abstract: The aim of the study was to investigate the effects of dietary fat on quality of liquid and frozen-thawed semen of Nili-Ravi buffalo bulls. Adult bulls (n=21) were fed a balanced ration (Con; n=7) or the same ration either containing sunflower oil (SF-O; n=7) or whole sunflower seeds (SF-S; n=7) for 63 days. Body weight and body condition score of each bull was recorded on days 0, 30 and 60 of the experiment. Semen was collected on days 39, 46, 53 and 60, frozen by a fast method and stored at -196 degrees C for 24h. Sperm motility was assessed using a bright field microscope. Plasma membrane integrity of fresh and frozen-thawed spermatozoa was assessed using a hypo-osmotic swelling (HOS) assay. The concentration of spermatozoa and volume of semen was not different among groups on various days of collection. Sunflower-enriched diets did not affect the motility and number of HOS-positive spermatozoa in the fresh semen. Motility and HOS of post-thawed spermatozoa were higher (p<0.05) in bulls fed the sunflower-enriched diets. Similarly, diets did not affect the body condition score and body weight of bulls. In conclusion, feeding of sunflower oil or sunflower seed as fat sources can improve the quality of buffalo bull spermatozoa.
authors.affiliation: Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Adeel
authors.forename: A
authors.initials: A
authors.lastname: Ijaz
authors.forename: M
authors.initials: M
authors.lastname: Aleem
authors.forename: H
authors.initials: H
authors.lastname: Rehman
authors.forename: M S
authors.initials: MS
authors.lastname: Yousaf
authors.forename: M A
authors.initials: MA
authors.lastname: Jabbar
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D050218:Body Fat Distribution; D002020:Buffaloes / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods; D004041:Dietary Fats / Q000494:pharmacology*; D005615:Freezing; D006368:Helianthus / Q000737:chemistry / Q000502:physiology; D008297:Male; D010938:Plant Oils / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry / Q000502:physiology; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects*; D000074242:Sunflower Oil
chemical_list: D004041:Dietary Fats; D010938:Plant Oils; D000074242:Sunflower Oil