The expression of P2Y1, 2, 4, and 6 receptors in rat endolymphatic sac epithelia.
Earlier studies have suggested that activation of the purinergic receptor causes Na absorption from the endolymph through nonselective cation channels in endolymphatic sac (ES) epithelia. In this study, the mRNA expression patterns of the P2Y(1, 2, 4, and 6) receptors in the rat ES were examined by conventional reverse-transcription PCR. Their cellular localization was investigated by high-specificity reverse-transcription PCR using laser capture microdissection and in-situ hybridization. Our experiments showed that the mRNA of these receptors is expressed in ES epithelia. These results indicate that extracellular nucleotides may regulate ion transport by several purinergic pathways that operate through these receptors in the ES and that some of these receptors may be responsible for regulating Na+ absorption through the activation of nonselective cation channels.
Mori
Terushige
T
Department of Otolaryngology, Kagawa University, Kagawa, Japan. morinko@med.kagawa-u.ac.jp
Miyashita
Takenori
T
Akiyama
Kosuke
K
Inamoto
Ryuhei
R
Mori
Nozomu
N
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism; D004712:Endolymphatic Sac / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D042282:Microdissection; D008840:Microfilament Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058472:Receptors, Purinergic P2Y1; D058473:Receptors, Purinergic P2Y2; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000096985:Calponins
D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1; D058473:Receptors, Purinergic P2Y2; D000096985:Calponins; C546384:P2ry1 protein, rat; C546387:P2ry2 protein, rat; C104749:purinoceptor P2Y4; C099845:purinoceptor P2Y6