title: [Clinical implication of expression of perforin/granzyme in peripheral blood lymphocyte in severe hepatitis B].
abstract: OBJECTIVE
  To explore role of perforin/granzyme expression of peripheral blood lymphocyte in injury of hepatocyte in severe hepatitis B, and evaluate relationship between perforin/granzyme expression levels and hepatitis B virus (HBV)-DNA load.
  
  
  METHODS
  Thirty eight patients of severe hepatitis B were enrolled in the study. Fasting venous blood was collected on following morning of admission. Twenty adult healthy subjects served as healthy control group. Perforin/granzyme expression of peripheral blood lymphocyte was detected by flow cytometry, and serum HBV-DNA load was detected by fluorescence quantitative polymerase chain reaction (PCR).
  
  
  RESULTS
  Positive rate of perforin/granzyme in peripheral blood lymphocyte in severe hepatitis B was higher than that of the healthy control group [perforin: (43.42+/-19.28)% vs. (19.65+/-9.27)%, granzyme: (40.35+/-12.26)% vs. (22.28+/-9.35)%, both P<0.01]. There was a significant negative correlation between the perforin/granzyme expression of peripheral blood lymphocyte and serum HBV-DNA load (r(perforin) =-0.92, r(granzyme) =-0.96, both P<0.01), the higher serum HBV-DNA load, the lower perforin/granzyme expression in severe hepatitis B.
  
  
  CONCLUSIONS
  Perforin/granzyme overexpression in peripheral blood lymphocyte is an important factor in injury of hepatocyte in patients with severe hepatitis B, and the expression may be involved in HBV-DNA cleanup.
authors.affiliation: Jinan Infectious Diseases Hospital, Jinan 250021, Shandong, China.
authors.forename: Su-nan
authors.initials: SN
authors.lastname: Cui
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Wang
authors.forename: Jing-wen
authors.initials: JW
authors.lastname: Liu
authors.forename: Zhao-hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Shu-li
authors.initials: SL
authors.lastname: Yu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000097:blood; D005260:Female; D053804:Granzymes / Q000097:blood*; D006509:Hepatitis B / Q000097:blood*; D006515:Hepatitis B virus; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054353:Perforin / Q000097:blood*; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D054353:Perforin; D053804:Granzymes