Aqueous batch rebinding and selectivity studies on sucrose imprinted polymers.
Polymers imprinted with sucrose and corresponding non-imprinted polymers are prepared photo-chemically at 3 degrees C and thermally at 65 degrees C. The pre-polymerization complex formation in dimethyl sulfoxide between sucrose and methacrylic acid via hydrogen bonding was investigated through (1)H NMR titration. The imprinting effect and the selectivity of the imprinted polymers in water are studied by batch rebinding studies with different mono and disaccharides and fitted to the Freundlich isotherm. Based on the calculated numbers of binding sites and average affinity, it is concluded that sucrose has been successfully imprinted at 3 and 65 degrees C. The polymer imprinted at 3 degrees C possesses the best recognition properties. The imprinted polymers are selective towards sucrose in water.
Kirk
Camilla
C
Department of Biotechnology, Aalborg University, DK-9000 Aalborg, Denmark.
Jensen
Martin
M
Kjaer
Christina N
CN
Smedskjaer
Morten M
MM
Larsen
Kim Lambertsen
KL
Wimmer
Reinhard
R
Yu
Donghong
D
D001665:Binding Sites; D015374:Biosensing Techniques; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D013395:Sucrose / Q000032:analysis / Q000737:chemistry*; D014867:Water
D002241:Carbohydrates; D008689:Methacrylates; D011108:Polymers; D011109:Polymethacrylic Acids; D014867:Water; D013395:Sucrose; C004919:ethylene dimethacrylate