An endophytic taxol-producing fungus from Taxus x media, Aspergillus candidus MD3.
An endophytic taxol-producing fungus (strain MD3) isolated from the inner bark of Taxus x media was identified as Aspergillus candidus according to its morphological characteristics, physiological and biochemical characteristics, and 18S rRNA gene sequence analysis. Taxol produced by A. candidus MD3 was shown to be identical to authentic taxol analyzed by UV, HPLC, MS and nuclear magnetic resonance spectroscopy. The gene encoding the 10-deacetylbaccatin III-10-O-acetyl transferase, which catalyzes formation of the last diterpene intermediate in the taxol biosynthetic pathway, has been cloned from A. candidus MD3 for the first time and possesses high homology to the same gene found in Taxus spp.
Zhang
Peng
P
Key Laboratory of Molecular Biophysics Ministry of Education, Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Zhou
Peng-Peng
PP
Yu
Long-Jiang
LJ
D000123:Acetyltransferases / Q000235:genetics; D001230:Aspergillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D020459:Genes, rRNA; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000737:chemistry / Q000378:metabolism*; D024301:Plant Bark / Q000382:microbiology; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013056:Spectrophotometry, Ultraviolet; D020946:Taxus / Q000382:microbiology*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D000123:Acetyltransferases; D017239:Paclitaxel