title: Differential CCR4 expression and function in cutaneous T-cell lymphoma cell lines.
abstract: Cutaneous T cell lymphoma (CTCL) is a clonal epidermotropic malignancy of memory T cells primarily involving the skin. However, the mechanisms governing migration of CTCL cells have not been fully clarified. It has been shown that certain chemokine receptors are upregulated in CTCL cells, but it remains unanswered whether these chemokine receptors play a critical role in the migration dynamics of CTCL. Using cell lines originally derived from patients with different subtypes of CTCL, we have shown higher CCR4 expression in the line derived from the mycosis fungoides (MJ), compared with the line derived from Sezary syndrome (Hut78). In specific responses to CCL22 (a CCR4 ligand) treatments, MJ cells showed significant chemotactic migration, enhanced activation and adhesion of certain integrins (CD49d and CD29) in vitro, while the control cells (Hut78, CD4+CD45RO+ memory T cells, and Jurkat cells) did not. Furthermore, compared with Hut78 cells, MJ cells manifested significantly more transendothelial migration in responses to treatments with either CCL22 or conditioned medium from dendritic cells in vitro. These results provide further dynamic evidence, in line with the multistep cascade paradigm for leukocyte transendothelial migration, to support a critical role for CCR4 in CTCL migration.
authors.affiliation: Department of Dermatology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Chieh-Shan
authors.initials: CS
authors.lastname: Wu
authors.forename: Sin-Ting
authors.initials: ST
authors.lastname: Wang
authors.forename: Chai-Yu
authors.initials: CY
authors.lastname: Liao
authors.forename: Meng-Tse
authors.initials: MT
authors.lastname: Wu
mesh_terms: D002448:Cell Adhesion; D054422:Chemokine CCL22 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D019743:Chemokines, CXC; D002633:Chemotaxis; D003713:Dendritic Cells / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D039041:Integrin alpha4beta1 / Q000378:metabolism; D008024:Ligands; D016410:Lymphoma, T-Cell, Cutaneous / Q000378:metabolism* / Q000473:pathology*; D009182:Mycosis Fungoides / Q000378:metabolism; D011485:Protein Binding; D054398:Receptors, CCR4 / Q000378:metabolism*; D015398:Signal Transduction; D012871:Skin Diseases / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: C515354:CCR4 protein, human; C508524:CXCL17 protein, human; D054422:Chemokine CCL22; D018925:Chemokines; D019743:Chemokines, CXC; D039041:Integrin alpha4beta1; D008024:Ligands; D054398:Receptors, CCR4