Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene.
We first reported that polyvinylpyrrolidone-protected graphene was dispersed well in water and had good electrochemical reduction toward O(2) and H(2)O(2). With glucose oxidase (GOD) as an enzyme model, we constructed a novel polyvinylpyrrolidone-protected graphene/polyethylenimine-functionalized ionic liquid/GOD electrochemical biosensor, which achieved the direct electron transfer of GOD, maintained its bioactivity and showed potential application for the fabrication of novel glucose biosensors with linear glucose response up to 14 mM.
Shan
Changsheng
C
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, and Graduate University of the Chinese Academy of Sciences, Changchun 130022, PR China.
Yang
Huafeng
H
Song
Jiangfeng
J
Han
Dongxue
D
Ivaska
Ari
A
Niu
Li
L
D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004579:Electron Transport; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D052578:Ionic Liquids / Q000737:chemistry; D010084:Oxidation-Reduction; D011094:Polyethyleneimine / Q000737:chemistry; D011205:Povidone / Q000737:chemistry
D052578:Ionic Liquids; D002244:Carbon; D011094:Polyethyleneimine; D005949:Glucose Oxidase; D011205:Povidone; D005947:Glucose