Antimicrobial susceptibility profiles of Salmonella enterica serotypes recovered from pens of commercial feedlot cattle using different types of composite samples.
Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). By characterizing Salmonella isolates from commercial feedlot pens, this study aimed to evaluate the strain diversity and prevalence of MDR Salmonella from different types of composite pen samples. Antimicrobial susceptibility profiles, serotype, and presence or absence of the integron-encoded intI1 gene were determined for 530 Salmonella isolates recovered using composite rope (n = 335), feces (n = 59), and water (n = 136) samples from 21 pens in 3 feedlots. The study investigated only pens with available isolates from multiple sample types. Most isolates (83.0%) of the 19 Salmonella serotypes identified were susceptible or intermediately susceptible to all the antimicrobials evaluated. Resistance to sulfisoxazole (14.9%), streptomycin (3.8%), and tetracycline (3.6%) were the most common. None of the isolates tested positive for a class 1 integron, and only 2.5% were resistant to multiple antimicrobials. All the MDR isolates, namely, serotypes Uganda (n = 9), Typhimurium (n = 2), and Give (n = 2), were resistant to at least five antimicrobials. Most MDR isolates (n = 11) were from two pens during 1 week within one feedlot. Overall, many Salmonella isolates collected within a pen were similar in terms of serotype and antimicrobial susceptibility regardless of sample type. However, MDR Salmonella and rare serotypes were not recovered frequently enough to suggest a general strategy for appropriate composite sampling of feedlot cattle populations for Salmonella detection and monitoring.
Alam
Mohammad Jahangir
MJ
Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS, USA.
Renter
David
D
Taylor
Ethel
E
Mina
Diana
D
Moxley
Rodney
R
Smith
David
D
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000940:Antigenic Variation; D002353:Carrier State; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D019426:Integrases / Q000235:genetics*; D008372:Manure / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D015146:Midwestern United States / Q000453:epidemiology; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping; D014871:Water Microbiology
D000890:Anti-Infective Agents; D008372:Manure; D019426:Integrases; C115006:integron integrase IntI1