Maritalea myrionectae gen. nov., sp. nov., isolated from a culture of the marine ciliate Myrionecta rubra.
A Gram-negative, rod-shaped, strictly aerobic bacterium, designated strain CL-SK30(T), was isolated from a culture of the marine ciliate Myrionecta rubra. 16S rRNA gene sequence analysis revealed that strain CL-SK30(T) was most closely related to Cucumibacter marinus (92.0 % similarity) and next to the type strains of species of the genus Devosia (89.8-91.3 % similarities) in the family Hyphomicrobiaceae. Phylogenetic analyses of the 16S rRNA gene sequences showed that strain CL-SK30(T) formed a robust clade together with C. marinus, but the sequence divergence value of 8 % between them indicated that the novel bacterium represented a distinct lineage. Strain CL-SK30(T) grew optimally in the presence of 2-5 % sea salts at 30-35 degrees C and pH 7.2-8.0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. Ubiquinone 10 was the major quinone. The DNA G+C content was 52.7 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain CL-SK30(T) represents a novel genus and species of the family Hyphomicrobiaceae, for which the name Maritalea myrionectae gen. nov., sp. nov. is proposed. The type strain is CL-SK30(T) (=KCCM 90060(T)=DSM 19524(T)).
Hwang
Chung Y
CY
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Cho
Kyung D
KD
Yih
Wonho
W
Cho
Byung C
BC
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016798:Ciliophora / Q000254:growth & development / Q000382:microbiology*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D041921:Hyphomicrobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S