title: Isolation of azaspiracid-2 from a marine sponge Echinoclathria sp. as a potent cytotoxin.
abstract: Azaspiracid-2 was isolated from a marine sponge Echinoclathria sp. collected off Amami-Oshima as the predominant cytotoxic constituent. A combination of HPLC using ODS, GS320, and Phenylhexyl stationary phases permitted the purification without using acid or inorganic additives in the mobile phase. Azaspiracid-2 exhibited potent cytotoxicity against P388 cells with an IC50 value of 0.72 ng/mL and caused S phase arrest on the cell cycle.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Ueoka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ito
authors.forename: Miho
authors.initials: M
authors.lastname: Izumikawa
authors.forename: Satoko
authors.initials: S
authors.lastname: Maeda
authors.forename: Motoki
authors.initials: M
authors.lastname: Takagi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shin-ya
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Rob W M
authors.initials: RW
authors.lastname: van Soest
authors.forename: Shigeki
authors.initials: S
authors.lastname: Matsunaga
mesh_terms: D000818:Animals; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy; D011161:Porifera / Q000378:metabolism*; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000095702:Polyether Toxins
chemical_list: D003603:Cytotoxins; D005663:Furans; D011714:Pyrans; C520447:azaspiracid-2; D000095702:Polyether Toxins