Whole blood aggregation, coagulation, and markers of platelet activation in diet-induced diabetic C57BL/6J mice.
OBJECTIVE
Type 2 diabetes in humans is associated with hypercoaguability; however, little is known about platelet function in mouse models of type 2 diabetes used to study this disorder. Therefore, the purpose of this study was to examine platelet aggregation, coagulation, and markers of platelet activation in a diet-induced mouse model of type 2 diabetes.


METHODS
Four week old, male, C57BL/6J mice were randomized to a standard chow or high fat (60% beef lard) diet for 4 months. To examine platelet function we measured ADP-induced whole blood aggregometry, whole blood coagulation by thromboelastography, tail bleeding times, platelet microparticle and platelet expression of p-selectin and platelet expression of CD61 by flow cytometry.


RESULTS
Diabetic mice exhibited less aggregation (p<0.05), less coagulation (p<0.01), prolonged tail bleeding times (n.s.), and lower agonist stimulated platelet CD61 expression (p<0.001) compared to non-diabetic mice. There was no difference in platelet microparticle and platelet p-selectin expression.


CONCLUSIONS
Diet-induced type 2 diabetic mouse do not demonstrate the hypercoagulability and platelet activation typically observed in humans with this disorder. More studies are warranted to further explore platelet function in this mouse model; to determine their applicability for studying these alterations in type 2 diabetes.
Henry
Melissa
M
School of Nursing, The University of Northern Colorado, Office 3290 Gunter Hall, Greeley, CO 80639, USA. melissa.henry@unco.edu
Davidson
Lisa
L
Cohen
Zoe
Z
McDonagh
Paul F
PF
Nolan
Paul E
PE
Ritter
Leslie S
LS
D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology; D005434:Flow Cytometry; D039661:Integrin beta3 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation / Q000502:physiology*; D011897:Random Allocation; D013916:Thrombelastography
D004041:Dietary Fats; D039661:Integrin beta3; D019007:P-Selectin