title: Combination of p53AIP1 and survivin expression is a powerful prognostic marker in non-small cell lung cancer.
abstract: BACKGROUND
  p53AIP1 is a potential mediator of apoptosis depending on p53, which is mutated in many kinds of carcinoma. High survivin expression in non-small cell lung cancer is related with poor prognosis. To investigate the role of these genes in non-small cell lung cancer, we compared the relationship between p53AIP1 or survivin gene expression and the clinicopathological status of lung cancer.
  
  
  METHODS
  Forty-seven samples from non-small cell lung cancer patients were obtained between 1997 and 2003. For quantitative evaluation of RNA expression by PCR, we used Taqman PCR methods.
  
  
  RESULTS
  Although no correlation between p53AIP1 or survivin gene expression and clinicopathological factors was found, the relationship between survivin gene expression and nodal status was significant (p = 0.03). Overall survival in the p53AIP1-negative group was significantly worse than in the positive group (p = 0.04); however, although survivin expression was not a prognostic factor, the combination of p53AIP1 and survivin was a significant prognostic predictor (p = 0.04). In the multivariate cox proportional hazard model, the combination was an independent predictor of overall survival (p53AIP1 (+) survivin (+), HR 0.21, 95%CI = [0.01-1.66]; p53AIP1 (+) survivin (-), HR 0.01, 95%CI = [0.002-0.28]; p53AIP1 (-) survivin (-), HR 0.01, 95%CI = [0.002-3.1], against p53AIP1 (-) survivin (+), p = 0.03).
  
  
  CONCLUSIONS
  These data suggest that the combination of p53AIP1 and survivin gene expression may be a powerful tool to stratify subgroups with better or worse prognosis from the variable non-small cell lung cancer population.
authors.affiliation: Department of Surgery II, Faculty of Medicine, Oita University, Hasama, Yufu, Oita, Japan. yamashi1@med.oita-u.ac.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Yamashita
authors.forename: Masao
authors.initials: M
authors.lastname: Chujo
authors.forename: Michiyo
authors.initials: M
authors.lastname: Miyawaki
authors.forename: Keita
authors.initials: K
authors.lastname: Tokuishi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Anami
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Katsunobu
authors.initials: K
authors.lastname: Kawahara
mesh_terms: D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C414742:P53AIP1 protein, human