Gonadotropin-releasing hormone modulates immune system function via the nuclear factor-kappaB pathway in murine Raw264.7 macrophages.
Gonadotropin-releasing hormone (GnRH), which was originally found to be involved in the reproductive process, has also been implicated in the modulation of immune system function. However, the underlying mechanisms of this involvement remain largely unclear. In this study, we found that GnRH increased the intracellular calcium levels in murine Raw264.7 macrophages. Furthermore, the production of nitric oxide, costimulated with lipopolysaccharide and interferon-gamma, was suppressed by exposure to GnRH. Moreover, the modulatory effects of GnRH on calcium and nitric oxide were observed in freshly isolated primary peritoneal macrophages. In addition, the activity of nuclear factor-kappaB was suppressed by GnRH exposure. On the other hand, the phosphorylation of the Janus kinase-signal transducer and activator of transcription pathway was not affected by cotreatment with GnRH. Taken together, these results demonstrate that GnRH participates in the macrophage function and indicate that the nuclear factor-kappaB signaling pathway may be responsible for GnRH-mediated immune system modulation.
Min
Joo Young
JY
Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Korea.
Park
Moon Ho
MH
Lee
Jae Kwan
JK
Kim
Hai Joong
HJ
Park
Yong Kyun
YK
D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism* / Q000494:pharmacology; D007155:Immunologic Factors / Q000378:metabolism* / Q000494:pharmacology; D018836:Inflammation Mediators / Q000494:pharmacology; D007371:Interferon-gamma / Q000187:drug effects / Q000276:immunology; D053613:Janus Kinase 1 / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism*; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000276:immunology*; D009569:Nitric Oxide / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*
D007155:Immunologic Factors; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009569:Nitric Oxide; D007987:Gonadotropin-Releasing Hormone; D007371:Interferon-gamma; C507905:Jak1 protein, mouse; D053613:Janus Kinase 1