Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein (MRP) affects transmigration in activated RAW264.7 cells.
The molecular action mechanism of MRP, one of the protein kinase C (PKC) substrates, has been under intense investigation, but reports on its role in macrophage function remain controversial. The treatment of macrophage cell lines with bacterial lipopolysaccharide (LPS) induced a high level of MRP expression suggesting that MRP plays a role in the function of activated macrophages. In order to investigate the role of MRP in activated RAW264.7 cells, we stably transfected MRP-specific shRNA expression constructs and tested for alterations in macrophage-related functions. The down-regulation of MRP expression resulted in a marked reduction in chemotaxis toward MCP-1 or extracellular matrix proteins. Furthermore, pharmacological inhibitors of PKC significantly inhibited the chemotaxis in RAW264.7 cells. These data reveals the pivotal role of MRP in the transmigration of activated RAW264.7 cells.
Chun
Kwang-Rok
KR
Department of Genetic Engineering, Kyungpook National University, Daegu, Republic of Korea.
Bae
Eun Mi
EM
Kim
Jae-Kwan
JK
Suk
Kyoungho
K
Lee
Won-Ha
WH
D000818:Animals; D001483:Base Sequence; D002148:Calmodulin-Binding Proteins; D002460:Cell Line; D018932:Chemokine CCL2 / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins; D010587:Phagocytosis; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
D002148:Calmodulin-Binding Proteins; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; C512945:Marcksl1 protein, mouse; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse