In vitro derby imaging of cancer biomarkers using quantum dots.
Semiconductor quantum dots (QDs), which have broad absorption with narrow emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and the arginine-glycine-aspartic acid (targeting the integrin alpha(v)beta(3)) in cancer cells is reported. Simultaneous fluorescence imaging of cellular distribution of nucleolin and integrin alpha(v)beta(3) using QDs enables easy monitoring of separate targets in the cancer cells and the normal healthy cells. These results suggest the feasibility of a concurrent visualization of QD-based multiple cancer biomarkers using small molecules such as aptamer or peptide ligands.
Ko
Mee Hyang
MH
Department of Nuclear Medicine, Seoul National University College of Medicine, Yongon-dong, Jongno-gu, Republic of Korea.
Kim
Soonhag
S
Kang
Won Jun
WJ
Lee
Jung Hwan
JH
Kang
Hyungu
H
Moon
Sung Hwan
SH
Hwang
Do Won
DW
Ko
Hae Young
HY
Lee
Dong Soo
DS
D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D018613:Microscopy, Confocal; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D045663:Quantum Dots; D016601:RNA-Binding Proteins / Q000378:metabolism; D000097796:Nucleolin
D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D039302:Integrin alphaVbeta3; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C513936:AGRO 100; C047981:arginyl-glycyl-aspartic acid