title: Exogenous N-acetylglucosamine increases hyaluronan production in cultured human dermal fibroblasts.
abstract: Application of hyaluronan (HA) containing cosmetic products to the skin is reported to moisturize and restore elasticity thereby achieving an antiwrinkle effect. In the skin, HA can be synthesized by dermal fibroblasts and N-acetylglucosamine (NAG) is a precursor for HA biosynthesis in the body. To study the effects of exogenous NAG on HA production in human dermal fibroblasts, HA production and HA-synthesizing enzymes 1, 2 and 3 mRNA expression in cultured human dermal fibroblasts were measured by ELISA and RT-PCR, respectively. The results showed that NAG promoted HA production while had no effect on the expression of HA-synthesizing enzymes 1, 2 and 3 mRNA in human dermal fibroblasts.
authors.affiliation: Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, 116027 Dalian, People's Republic of China. tucx2003@163.com
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Tu
authors.forename: Rong-Xin
authors.initials: RX
authors.lastname: Zhang
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Tian
authors.initials: T
authors.lastname: Huang
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D002478:Cells, Cultured; D003358:Cosmetics; D020405:Dermis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D003358:Cosmetics; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; C523794:HAS1 protein, human; C101754:HAS2 protein, human; C105458:HAS3 protein, human; D000076002:Hyaluronan Synthases; D000117:Acetylglucosamine