Multivalent human blood group ABH and Lewis glycotopes are key recognition factors for a LFuc>Man binding lectin from phytopathogenic Ralstonia solanacearum.
Ralstonia solanacearum lectin (RSL), that might be involved in phytopathogenicity, has been defined as LFuc>>Man specific. However, the effects of polyvalency of glycotopes and mammalian structural units on binding have not been established. In this study, recognition factors of RSL were comprehensively examined with natural multivalent glycotopes and monomeric ligands using enzyme linked lectin-sorbent and inhibition assays. Among the glycans tested, RSL reacted strongly with multivalent blood group A(h) (GalNAcalpha1-3[Fucalpha1-2]Gal) and H (Fucalpha1-2Gal) active glycotopes, followed by B(h) (Galalpha1-3[Fucalpha1-2]Gal), Le(a) (Galbeta1-3[Fucalpha1-4]GlcNAc) and Le(b) (Fucalpha1-2Galbeta1-3[Fucalpha1-4]GlcNAc) active glycotopes. But weak or negligible binding was observed for blood group precursors having Galbeta1-3/4GlcNAcbeta1- (Ibeta/IIbeta) residues or Galbeta1-3GalNAcalpha1- (Talpha), GalNAcalpha1-Ser/Thr (Tn) bearing glycoproteins. These results indicate that the density and degree of exposure of multivalent ligands of alpha1-2 linked LFuc to Gal at the non-reducing end is the most critical factor for binding. An inhibition study with monomeric ligands revealed that the combining site of RSL should be of a groove type to fit trisaccharide binding with highest complementarity to blood group H trisaccharide (H(L); Fucalpha1-2Galbeta1-4Glc). The outstandingly broad RSL saccharide-binding profile might be related to the unusually wide spectrum of plants that suffer from R. solanacearum pathogenicity and provide ideas for protective antiadhesion strategies.
Wu
Albert M
AM
Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-san, Tao-yuan, Taiwan. amwu@mail.cgu.edu.tw
Wu
June H
JH
Singh
Tanuja
T
Singha
Biswajit
B
Sudakevitz
Dvora
D
Gilboa-Garber
Nechama
N
D000017:ABO Blood-Group System / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000737:chemistry*; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry*; D013552:Swine; D014312:Trisaccharides / Q000737:chemistry
D000017:ABO Blood-Group System; D004187:Disaccharides; D007983:Lewis Blood Group Antigens; D009077:Mucins; D037121:Plant Lectins; D014312:Trisaccharides; C090128:fucosyl-1-2-mannose; C031420:2'-fucosyllactose