title: Synchronous fluorescence determination of urinary 1-hydroxypyrene, beta-naphthol and 9-hydroxyphenanthrene based on the sensitizing effect of beta-cyclodextrin.
abstract: A novel method for the simultaneous determination of 1-hydroxypyrene (1-OHP), beta-naphthol (beta-NAP) and 9-hydroxyphenanthrene (9-OHPe) in human urine has been established by using synchronous fluorescence spectrometry. It was based on the fact that synchronous fluorescence spectrometry can resolve the broad-band overlapping of conventional fluorescence spectra, which arise from their similar molecular structures. Only one single scan is needed for quantitative determination of three compounds simultaneously when Deltalambda=15nm is chosen. The signals detected at these three wavelengths, 369.6, 330.0 and 358.0nm, vary linearly when the concentration of 1-OHP, beta-NAP and 9-OHPe is in the range of 2.16x10(-8)-1.50x10(-5)molL(-1), 1.20x10(-7)-1.10x10(-5)molL(-1) and 1.07x10(-7)-3.50x10(-5)molL(-1), respectively. The correlation coefficients for the standard calibration graphs were 0.994, 0.999 and 0.997 (n=7) for 1-OHP, beta-NAP and 9-OHPe, respectively. The limits of detection (LOD) for 1-OHP, beta-NAP and 9-OHPe were 6.47x10(-9)molL(-1), 3.60x10(-8)molL(-1) and 3.02x10(-8)molL(-1)with relative standard deviations (R.S.D.) of 4.70-6.40%, 2.80-4.20%, 3.10-4.90% (n=6), respectively. The method described here had been applied to determine traces of 1-OHP, beta-NAP and 9-OHPe in human urine, and the obtained results were in good agreement with those obtained by the HPLC method. In addition, the interaction modes between beta-cyclodextrin (beta-CD) and 1-OHP, beta-NAP or 9-OHPe, as well as the mechanism of the fluorescence enhancement were also discussed.
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Yang
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Li
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Tan
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Xue
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Xiao
authors.forename: Rong-Hui
authors.initials: RH
authors.lastname: Kang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009284:Naphthols / Q000652:urine*; D010616:Phenanthrenes / Q000652:urine*; D011721:Pyrenes / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013816:Thermodynamics; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D009284:Naphthols; D010616:Phenanthrenes; D011721:Pyrenes; D047392:beta-Cyclodextrins; C091375:9-phenanthrol; C031215:betadex; C033146:1-hydroxypyrene; C028405:2-naphthol