Dietary taurine attenuates dextran sulfate sodium (DSS)-induced experimental colitis in mice.
Effects of dietary taurine on the experimental colitis induced by dextran sulfate sodium (DSS) were studied. C57BL/6 mice administrated taurine or placebo for 5 days were given 3% DSS to induce acute. The colitis was as-sessed using indices such as diarrhea/bleeding scores, colon length change, histological score and tissue myeloperoxidase (MPO) activity. Further, tissue mRNA levels of interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and macrophage inflammatory protein (MIP)-2, were determined by real-time PCR. Taurine supplementation significantly attenuated the severity of diarrhea, colon shortening, histological score, MPO activity elevation and abnormal MIP-2 gene expression, indicating that taurine prevents DSS-induced colitis. Taurine also inhibited the TNF-alpha-induced secretion of IL-8 (a human homologue of MIP-2) from human intestinal epithelial Caco-2 cells. Inhibition of chemokine secretion from intestinal cells may be involved in the mechanisms underlying the cytoprotective function of taurine in the intestinal epithelium.
Shimizu
Makoto
M
Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, Japan. ams316@mail.ecc.u-tokyo.ac.jp
Zhao
Zhaohui
Z
Ishimoto
Yoko
Y
Satsu
Hideo
H
D000818:Animals; D016264:Dextran Sulfate / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004032:Diet; D004195:Disease Models, Animal; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D016209:Interleukin-8 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D013654:Taurine / Q000008:administration & dosage / Q000494:pharmacology*
D016209:Interleukin-8; D013654:Taurine; D016264:Dextran Sulfate; D009195:Peroxidase