title: Cross-inhibition between native and recombinant TRPV1 and P2X(3) receptors.
abstract: Small- to medium-sized neurons in the dorsal root ganglion (DRG) convey nociceptive information to the spinal cord. The co-expression of TRPV1 receptors (sensitive to vanilloids, heat and acidic pH) with P2X(3) receptors (sensitive to extracellular ATP) has been found in many DRG neurons. We investigated whether the co-activation of these two receptor classes in small-diameter cells leads to a modulation of the resulting current responses shaping the intensity of pain sensation. The whole-cell patch clamp method was used to record agonist-induced currents in cultured rat DRG neurons and in HEK293 cells transfected with the respective wild-type recombinant receptors or their mutants. Co-immunoprecipitation studies were used to demonstrate the physical association of TRPV1 and P2X(3) receptors. At a negative holding potential, the P2X(3) receptor agonist alpha,beta-meATP induced less current in the presence of the TRPV1 agonist capsaicin than that in its absence. This inhibitory interaction was not changed at a positive holding potential, in a Ba(2+)-containing superfusion medium, or when the buffering of intrapipette Ca(2+) was altered. The C-terminal truncation at Glu362 of P2X(3) receptors abolished the TRPV1/P2X(3) cross-talk in the HEK293 expression system. Co-immunoprecipitation studies with polyclonal antibodies generated against TRPV1 and P2X(3) showed a visible signal in HEK293 cells transfected with both receptors. It is concluded that the two pain-relevant receptors TRPV1 and P2X(3) interact with each other in an inhibitory manner probably by a physical association established by a motif located at the C-terminal end of the P2X(3) receptor distal to Glu362.
authors.affiliation: Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Haertelstrasse 16-18, D-04107 Leipzig, Germany.
authors.forename: Doychin
authors.initials: D
authors.lastname: Stanchev
authors.forename: Maren
authors.initials: M
authors.lastname: Blosa
authors.forename: Doreen
authors.initials: D
authors.lastname: Milius
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Gerevich
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rubini
authors.forename: Günther
authors.initials: G
authors.lastname: Schmalzing
authors.forename: Klaus
authors.initials: K
authors.lastname: Eschrich
authors.forename: Michael
authors.initials: M
authors.lastname: Schaefer
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wirkner
authors.forename: Peter
authors.initials: P
authors.lastname: Illes
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D009433:Neural Inhibition / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism*; D058477:Receptors, Purinergic P2X3; D011994:Recombinant Proteins / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: C546407:P2RX3 protein, human; C546410:P2rx3 protein, rat; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D011994:Recombinant Proteins; D050916:TRPV Cation Channels; C400769:TRPV2 protein, human; C493922:Trpv1 protein, rat