Extraction of two active polysaccharides from the yeast cell wall.
Yeast cell wall matrix particles are composed entirely of beta-glucan and mannoprotein. Alkali-insoluble (1-->3)-beta-D-glucan was extracted from the yeast cell wall by an alkaline-acid method. IR spectra analysis showed that the product was chemically pure glucan, that is to say, it contained no other carbohydrates and proteins. So, the alkaline-acid method was ideal for extracting (1-->3)-beta-D-glucan from the yeast cell wall. We also purified and analyzed mannan oligosaccharides by the dilute alkali-Sevage method from the yeast cell wall.
Huang
Gang L
GL
College of Chemistry, Chongqing Normal University, Chongqing 400047, China. huangdoctor226@163.com
D002473:Cell Wall / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D047071:beta-Glucans / Q000737:chemistry / Q000302:isolation & purification
D008351:Mannans; D011134:Polysaccharides; D047071:beta-Glucans