title: Subversion of pulmonary dendritic cell function by paramyxovirus infections.
abstract: Lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the Ag-presenting function of the lung which follow these infections. In this study, we investigated whether hMPV and RSV experimental infections alter the phenotype and function of dendritic cell (DC) subsets that are recruited to the lung. Characterization of lung DC trafficking demonstrated a differential recruitment of plasmacytoid DC (pDC), conventional DC (cDC), and IFN-producing killer DC to the lung and draining lymph nodes after hMPV and RSV infection. In vitro infection of lung DC indicated that in pDC, production of IFN-alpha, TNF-alpha, and CCL5 was induced only by hMPV, whereas CCL3 and CCL4 were induced by both viruses. In cDC, a similar repertoire of cytokines was induced by hMPV and RSV, except for IFN-beta, which was not induced by RSV. The function of lung pDC was altered following hMPV or RSV infection in vivo, as we demonstrated a reduced capacity of lung pDC to produce IFN-alpha as well as other cytokines including IL-6, TNF-alpha, CCL2, CCL3, and CCL4 in response to TLR9 stimulation. Moreover, we observed an impaired capacity of cDC from infected mice to present Ag to CD4(+) T cells, an effect that lasted beyond the acute phase of infection. Our findings suggest that acute paramyxovirus infections can alter the long-term immune function of pulmonary DC.
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch, Galveston, TX 77555, USA. maaguerr@utmb.edu
authors.forename: Antonieta
authors.initials: A
authors.lastname: Guerrero-Plata
authors.forename: Deepthi
authors.initials: D
authors.lastname: Kolli
authors.forename: Chao
authors.initials: C
authors.lastname: Hong
authors.forename: Antonella
authors.initials: A
authors.lastname: Casola
authors.forename: Roberto P
authors.initials: RP
authors.lastname: Garofalo
mesh_terms: D000208:Acute Disease; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D029121:Metapneumovirus / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D018184:Paramyxoviridae Infections / Q000276:immunology*; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D016898:Interferon-alpha