title: Roles of the creatine kinase system and myoglobin in maintaining energetic state in the working heart.
abstract: BACKGROUND
  The heart is capable of maintaining contractile function despite a transient decrease in blood flow and increase in cardiac ATP demand during systole. This study analyzes a previously developed model of cardiac energetics and oxygen transport to understand the roles of the creatine kinase system and myoglobin in maintaining the ATP hydrolysis potential during beat-to-beat transient changes in blood flow and ATP hydrolysis rate.
  
  
  RESULTS
  The theoretical investigation demonstrates that elimination of myoglobin only slightly increases the predicted range of oscillation of cardiac oxygenation level during beat-to-beat transients in blood flow and ATP utilization. In silico elimination of myoglobin has almost no impact on the cytoplasmic ATP hydrolysis potential (DeltaGATPase). In contrast, disabling the creatine kinase system results in considerable oscillations of cytoplasmic ADP and ATP levels and seriously deteriorates the stability of DeltaGATPase in the beating heart.
  
  
  CONCLUSIONS
  The CK system stabilizes DeltaGATPase by both buffering ATP and ADP concentrations and enhancing the feedback signal of inorganic phosphate in regulating mitochondrial oxidative phosphorylation.
authors.affiliation: Biotechnology and Bioengineering Center, Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA. fwu@mcw.edu
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Beard
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004734:Energy Metabolism; D006321:Heart / Q000502:physiology*; D006868:Hydrolysis; D008954:Models, Biological; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009211:Myoglobin / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009211:Myoglobin; D000255:Adenosine Triphosphate; D003402:Creatine Kinase