title: Metabolic pathway analysis of glycerol metabolism in Klebsiella pneumoniae incorporating oxygen regulatory system.
abstract: It is well known that environmental and genetic perturbations have major effects on the metabolic behavior of cells. In this work, a model that utilizes existing knowledge of oxygen and redox sensing/regulatory system to assist elementary flux modes (EFMs) has been developed and was carried out to predict the metabolic potential of Klebsiella pneumoniae for the production of 1,3-propanediol (1,3-PD) under anaerobic and aerobic conditions. It was found that the theoretical optimal 1,3-PD yield could reach to 0.844 mol mol(-1) if the pentose phosphate pathway (PPP), and transhydrogenase had a high flux under anaerobic condition. However, PPP had little influence on the theoretical 1,3-PD yield, and the flux through tricarboxylic acid (TCA) cycle was high under aerobic conditions. In addition, the effect of oxygen level on the 1,3-PD and biomass was further analyzed.
authors.affiliation: Dept. of Bioscience and Biotechnology, Dalian University of Technology, Linggong Road 2, Dalian 116023, P.R. China.
authors.forename: Qingrui
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Xiu
mesh_terms: D002952:Citric Acid Cycle / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000502:physiology; D011409:Propylene Glycols / Q000378:metabolism
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol; D010100:Oxygen