YB-1 transcription in the postnatal brain is regulated by a bHLH transcription factor Math2 through an E-box sequence in the 5'-UTR of the gene.
YB-1 controls gene expression at both the transcriptional and translational levels. In the brain, a high level of YB-1 is expressed from prior to the first week after birth. However, YB-1 expression substantially declines to the adult level thereafter. Here, we investigated the regulatory mechanism of YB-1 transcription in the postnatal brain. We found that an E-box sequence within the 5'-untranslated region (5'-UTR) of the gene was recognized by Math2 in the 5-day-old brain, whereas the DNA binding was reduced in the 4-week-old brain. In vitro transcription analysis and reporter gene assay revealed that Math2 was necessary for YB-1 transcription. Furthermore, the expression pattern of Math2 protein in the postnatal brain was correlated with that of YB-1 protein. Our results suggest that YB-1 transcription in the postnatal brain is regulated by Math2, which binds to an E-box in the 5'-UTR of the YB-1 gene.
Ohashi
Sachiyo
S
Research Unit of Biochemistry, College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba 274-8555, Japan.
Fukumura
Reiko
R
Higuchi
Takashi
T
Kobayashi
Shunsuke
S
D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D024721:E-Box Elements / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C110701:Neurod6 protein, mouse; C541744:Ybx1 protein, mouse