title: [Histologic study and type II collagen expression in the rib cartilage of patients at different age].
abstract: OBJECTIVE
  To study the difference of histologic examination and type II collagen expression in the rib cartilage of patients at different age.
  
  
  METHODS
  The patients were divided into three groups, as children group (5 to 10 years old), teenager group (11 to 17 years old), and adult group (18 to 29 years old). Histologic examination of rib cartilage and quantitative analysis of type II collagen with immunohistochemical stain were carried out in the three groups. Motic Med 6.0 A system was used to quantitate immunohistochemical image through measurement of mean photodensity. The results were statistically analyzed.
  
  
  RESULTS
  Among the three groups, the children's rib cartilage had the most blood vessels, the most chondrocytes, well-distributed stain of matrixes, and the type II collagen was expressed actively and highest in photodensity. The rib cartilage of teenager group had less blood vessels, unhomogeny distributed stain of matrixes, the enlarged and separated cartilage lacunae. The rib cartilage in adult group showed the least blood vessels, the least chondrocytes, the hyalinization of perichondrium, the most deposition of calcium salt, and the type II collagen was expressed at the lowest level in photodensity. Statistical analysis showed significant differences among the three groups (P < 0.01).
  
  
  CONCLUSIONS
  Histologic structure in human rib cartilage changes during the aging process and the type II collagen is decreased with the increase of age.
authors.affiliation: Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Yang
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Zhuang
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Jiang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhuang
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D019902:Chondrocytes / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D012272:Ribs / Q000033:anatomy & histology* / Q000378:metabolism*; D055815:Young Adult
chemical_list: D024043:Collagen Type II