[Diazoxide-induced mitochondrial swelling in the rat myometrium as a consequence of the activation of the mitochondrial ATP-sensitive (K+)-channel].
In this paper we postulate, that mitochondria isolated from the rat myometrium undergo swelling in isoosmotic medium, which contains K+ (125 mM). This swelling was blocked by ATP (200 microM), but only when MgCl2 (1 mM) was present and observed when oligomycin, the inhibitor of FoF1-ATPase, was added to the incubation medium. Diazoxide (50 microM), activator of the mitochondrial ATP-sensitive potassium channel, removed ATP-induced blockade of swelling. Our results may prompt the presence of K+ transporter on the inner mitochondrial membrane, which possesses the features of the mitochondrial ATP-dependent potassium channel described earlier in mitochondria of the heart, liver, brain, retina, blood vessels and kidneys.
Vadziuk
O B
OB
Kosterin
S A
SA
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D003981:Diazoxide / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D054086:KATP Channels / Q000378:metabolism*; D015636:Magnesium Chloride / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects*; D009215:Myometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D051381:Rats
D054086:KATP Channels; D009840:Oligomycins; D015636:Magnesium Chloride; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D003981:Diazoxide