Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms.
Talins are large adaptor proteins that link the integrin family of adhesion molecules to F-actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin-mediated cell adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian talin 2. This reveals the existence of a previously unrecognized promoter associated with a CpG island, and separated from the first coding exon by numerous alternatively spliced noncoding exons spanning > 200 kb. Analysis of a mouse gene trap line shows that this promoter accounts for most of the talin 2 expression in adult tissues. We also demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N-terminal half of the protein, and provide evidence for the developmentally regulated expression of the short testis-specific talin 2 isoform in elongating spermatids. Finally, we identify four tissue-specific alternative splicing events within the coding region of talin 2.
Debrand
Emmanuel
E
Department of Biochemistry, University of Leicester, Leicester, UK.
El Jai
Yasmine
Y
Spence
Lorraine
L
Bate
Neil
N
Praekelt
Uta
U
Pritchard
Catrin A
CA
Monkley
Susan J
SJ
Critchley
David R
DR
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D004247:DNA; D005091:Exons; D055785:Gene Knockdown Techniques; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013087:Spermatids / Q000378:metabolism; D016608:Talin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D012333:RNA, Messenger; C518833:TLN2 protein, mouse; D016608:Talin; D004247:DNA