Biogenic amine determination in wines using solid-phase extraction: a comparative study.
Biogenic amines in wine usually are analyzed by high-performance liquid chromatography after direct derivatization. A method of isolation based on solid-phase extraction (SPE) with mixed-mode resins (Oasis MCX, reverse-phase and ion exchange) was developed. The different stages of the isolation process (loading, elution and washing) were optimized to obtain a simple procedure that yields a clean chromatogram. The relative standard deviation (%RSD) of the retention times and relative areas was less than 0.3% and 6%, respectively. Limits of quantification were lower than 0.16 mg L(-1) for all the amines and the linear range of concentration was 0.16-8 mg L(-1) for putrescine, cadaverine and tyramine, and up to 10 mg L(-1) for histamine.
Peña-Gallego
A
A
Department of Analytical Chemistry, School of Sciences, University of Zaragoza, Zaragoza, Spain.
Hernández-Orte
P
P
Cacho
J
J
Ferreira
V
V
D001679:Biogenic Amines / Q000032:analysis*; D002853:Chromatography, Liquid; D005453:Fluorescence; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
D001679:Biogenic Amines