Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle.
Mixed lineage leukemias (MLLs) are histone H3 at lysine 4 (H3K4)-specific methylases that play a critical role in regulating gene expression in humans. As chromatin condensation, relaxation and differential gene expression are keys to correct cell cycle progression, we analyzed the dynamic association of MLL and H3K4 trimethylation at different stages of the cell cycle. Interestingly, MLL1, which is normally associated with transcriptionally active chromatins (G1 phase), dissociates from condensed mitotic chromatin and returns at the end of telophase when the nucleus starts to relax. In contrast, H3K4 trimethylation mark, which is also normally associated with euchromatins (in G1), remains associated, even with condensed chromatin, throughout the cell cycle. The global levels of MLL1 and H3K4 trimethylation are not affected during the cell cycle, and H3Ser28 phosphorylation is only observed during mitosis. Interestingly, MLL target homeobox-containing (Hox) genes (HoxA5, HoxA7 and HoxA10) are differentially expressed during the cell cycle, and the recruitment of MLL1 and H3K4 trimethylation levels are modulated in the promoter of these Hox genes as a function of their expression. In addition, down-regulation of MLL1 results in cell cycle arrest at the G2/M phase. The fluctuation of H3K4 trimethylation marks at specific promoters, but not at the global level, indicates that H3K4 trimethylation marks that are present in the G1 phase may not be the same as the marks in other phases of the cell cycle; rather, old marks are removed and new marks are introduced. In conclusion, our studies demonstrate that MLL1 and H3K4 methylation have distinct dynamics during the cell cycle and play critical roles in the differential expression of Hox genes associated with cell cycle regulation.
Mishra
Bibhu P
BP
Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington 76019, TX, USA.
Ansari
Khairul I
KI
Mandal
Subhrangsu S
SS
D001483:Base Sequence; D015153:Blotting, Western; D002453:Cell Cycle; D002843:Chromatin / Q000378:metabolism*; D017931:DNA Primers; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005801:Genes, Homeobox; D006367:HeLa Cells; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D051788:Myeloid-Lymphoid Leukemia Protein / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002843:Chromatin; D017931:DNA Primers; D006657:Histones; C497422:KMT2A protein, human; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase