[Effects of montelukast and BCG-PSN on the expression of STAT5b mRNA and IL-4 mRNA in blood mononuclearcells of rats with asthma].
OBJECTIVE
To study the expression of signal transducer and activator of transcription 5b (STAT5b) mRNA and interleukin-4 (IL-4) mRNA in blood mononuclearcells in a rat model of asthma and the effect of montelukast (MK) and BCG-polysaccharide and nucleic acid injection (BCG-PSN) on STAT5b mRNA and IL-4 mRNA expression.


METHODS
Fifty-two male Sprague-Dawley rats (weight:140-200 g) were randomly divided into four groups: asthma, MK treated and BCG-PSN-treated and control groups. Rat model of asthma was prepared by ovalbumin (OVA) sensitization. The rats were sacrificed 24 hrs after the last sensitization. Blood eosinophils (EOS) were counted. Plasma contens of IL-4 and interferon-gamma (IFN-gamma) were measured using ELISA. Expression of STAT5b mRNA and IL-4 mRNA in blood mononuclearcells was detected with SYBR GREEN I fluorescent quantitation PCR method.


RESULTS
Blood contents of STAT5b mRNA and IL-4 mRNA in the untreated asthma group were significantly higher than those in the other three groups (<0.01). Blood EOS count and plasma IL-4 contents in the untreated asthma group significantly increased, while plasma IFN-gamma contents significantly decreased compared with the other three groups (<0.01). There were no significant differences in the parameters measured among the MK-treated, the BCG-PSNjtreated and the control groups. STAT5b mRNA expression was positively correlated to IL-4 mRNA expression, IL-4 content and EOS count (r=0.730,0.650, 0.664, respectively; <0.01), but negatively correlated to IFN-gamma content (r=-0.798; <0.01).


CONCLUSIONS
STAT5b mRNA and IL-4 mRNA were strongly expressed in blood mononuclearcells in rats with asthma, and there was a positive correlation between them. MK and BCG-PSN had inhibitory effects on the expression of STAT5b mRNA and IL-4 mRNA, which might be contributed to suppression of airway inflammation in asthma.
Jin
Yao-Jian
YJ
Department of Immunology, Jinhua College of Profession and Technology, Jinhua, Zhejiang 321007, China. jinyj999@126.com
Song
Zhi-Yan
ZY
Hu
Ye
Y
Qian
Xu-Bo
XB
Wang
Xiao-Yang
XY
He
Xu-Ying
XY
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D001249:Asthma / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D001500:BCG Vaccine / Q000494:pharmacology*; D003521:Cyclopropanes; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D011804:Quinolines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050799:STAT5 Transcription Factor / Q000235:genetics*; D013440:Sulfides
D000085:Acetates; D001500:BCG Vaccine; D003521:Cyclopropanes; D011804:Quinolines; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; C494097:Stat5b protein, rat; D013440:Sulfides; D015847:Interleukin-4; D007371:Interferon-gamma; C093875:montelukast