Bioactive cycloartane-type triterpene glycosides from Astragalus elongatus.
Together with two known cycloartane-type glycosides, askendosides D (3-O-[alpha-arabinopyranosyl-(1-->2)-beta-xylopyranosyl]-6-O-beta-xylopyranosyl-cycloastragenol, 2) and G (3-O-[alpha-arabinopyranosyl-(1-->2)-beta-xylopyranosyl]-16-O-beta-glucopyranosyl-3 beta,6 alpha,16 beta,24(R),25-pentahydroxycycloartane, 3), also a new monodesmosidic cycloartane-type glycoside, elongatoside (1), was isolated from the roots of Astragalus elongatus and identified as 3-O-[alpha-arabinopyranosyl-(1-->2)-beta-xylopyranosyl]-cycloastragenol. All structures were unambiguously determined by means of spectroscopic and spectrometric methods (1D and 2D NMR, ESI-MS). The isolated compounds were tested for the inhibition of proliferation and ICAM-1 expression in vitro using the human microvascular endothelial cell line (HMEC-1). 1 showed weak activity in the ICAM-1 assay.
Caliş
Ihsan
I
Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, TR-06100 Ankara, Turkey.
Barbic
Matej
M
Jürgenliemk
Guido
G
D027883:Astragalus Plant / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008833:Microcirculation / Q000502:physiology; D008958:Models, Molecular; D008968:Molecular Conformation; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D006027:Glycosides; D010936:Plant Extracts; D014315:Triterpenes; D018799:Intercellular Adhesion Molecule-1; C085352:cycloartane