Protective effect of an extract of Emblica officinalis against radiation-induced damage in mice.
The radioprotective effect of Emblica officinalis extract (EOE) was studied in mice. Swiss albino mice were exposed to gamma rays (5 Gy) in the absence (control) or presence (experimental) of EOE, orally 100 mg/kg body weight, once daily for 7 consecutive days. A specimen of small intestine (jejunum) was removed from the mice and studied at different autopsy intervals from 12 hours to 30 days. In control animals, crypt cell population, mitotic figures, and villus length were markedly reduced on day 1; these later started to increase progressively but did not attain the normal level even at the last autopsy interval. The animals receiving EOE prior to irradiation had a higher number of crypt cells and mitotic figures when compared with non-drug-treated control at all the autopsy intervals. Irradiation of animals resulted in a dose-dependent elevation in lipid peroxidation and a reduction in glutathione as well as catalase concentration in the intestine at 1 hour post-irradiation. In contrast, EOE treatment before irradiation caused a significant depletion in lipid peroxidation and elevation in glutathione and catalase levels.
Jindal
Archana
A
Radiation and Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India.
Soyal
Dhanraj
D
Sharma
Abhilasha
A
Goyal
P K
PK
D000284:Administration, Oral; D000818:Animals; D002374:Catalase / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays / Q000009:adverse effects; D005978:Glutathione / Q000187:drug effects / Q000528:radiation effects; D007583:Jejunum / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D029842:Phyllanthus emblica / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D010936:Plant Extracts; D011837:Radiation-Protective Agents; D002374:Catalase; D005978:Glutathione