Identification of mutation sites on Delta12 desaturase genes from Mortierella alpina 1S-4 mutants.
The mutation sites on the Delta12 desaturase gene in Mortierella alpina Delta12 desaturase-defective mutants SR88, TM912, and Mut48 accumulating Mead acid were identified. Each mutation resulted in an amino acid replacement (H116Y and P166L) in the Delta12 desaturase gene from SR88 and Mut48, respectively.
Sakuradani
Eiji
E
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Abe
Takahiro
T
Matsumura
Kenji
K
Tomi
Akiko
A
Shimizu
Sakayu
S
D001483:Base Sequence; D017931:DNA Primers; D044943:Fatty Acid Desaturases / Q000235:genetics*; D020094:Mortierella / Q000201:enzymology*; D009154:Mutation
D017931:DNA Primers; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase