title: Altered postsynaptic-density-levels of caldendrin in the para-chloroamphetamine-induced serotonin syndrome but not in the rat ketamine model of psychosis.
abstract: Caldendrin is a synaptic calcium sensor protein that is tightly associated with the postsynaptic density (PSD). Previous work has shown that the association of the protein with the synapse is highly dynamic and is increased in an activity-dependent manner. In the present study the caldendrin-association with the postsynaptic cytomatrix was analyzed in animal models of psychosis and drug abuse induced neurotoxicity. Subchronic administration of the N-methyl-D-aspartate (NMDA)-receptor antagonist ketamine, serving as a model of NMDA-receptor hypofunction and schizophrenia showed no significant effect on the PSD-levels of caldendrin, indicating that NMDA-receptor activity is not required to keep caldendrin at the synapse. However, administration of high doses of the serotonergic neurotoxin p-chloroamphetamine (PCA) lead to significant changes in the association of caldendrin with the PSD. These results underscore the dynamic association of caldendrin with the PSD and suggest a role of this synaptic calcium sensor in the PCA-induced serotonin syndrome.
authors.affiliation: Special Lab Molecular Biological Techniques, Leibniz-Institute for Neurobiology, Magdeburg, Germany.
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Smalla
authors.forename: Jale
authors.initials: J
authors.lastname: Sahin
authors.forename: Jörg
authors.initials: J
authors.lastname: Putzke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tischmeyer
authors.forename: Eckart D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: Michael R
authors.initials: MR
authors.lastname: Kreutz
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists; D007649:Ketamine; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011605:Psychoses, Substance-Induced / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018490:Serotonin Agents; D020230:Serotonin Syndrome / Q000139:chemically induced* / Q000378:metabolism*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D010133:p-Chloroamphetamine
chemical_list: D002135:Calcium-Binding Proteins; D018691:Excitatory Amino Acid Antagonists; D009419:Nerve Tissue Proteins; D018490:Serotonin Agents; D010133:p-Chloroamphetamine; D007649:Ketamine; C085577:Ca2+-binding protein-1