Structure and dynamics of the human muscle LIM protein.
The family of cysteine rich proteins (CRP) comprises three closely homologous members that have been reported to interact with alpha-actinin. Muscular LIM protein (MLP/CRP3), the skeletal muscle variant, was originally discovered as a positive regulator of myogenesis and is suggested to be part of the stretch sensor of the myofibril through its interaction with telethonin (T-Cap). We determined the structure of both LIM domains of human MLP by nuclear magnetic resonance spectroscopy. We confirm by (15)N relaxation measurements that both LIM domains act as independent units and that the adjacent linker regions are fully flexible. With the published structures of CRP1 and CRP2, the complete family has now been structurally characterized.
Schallus
Thomas
T
Department of Biomolecular Mechanisms, Max-Planck-Institute for Medical Research, Heidelberg, Germany.
Fehér
Krisztina
K
Ulrich
Anne S
AS
Stier
Gunter
G
Muhle-Goll
Claudia
C
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D005136:Eye Proteins / Q000737:chemistry; D018398:Homeodomain Proteins / Q000737:chemistry; D006801:Humans; D060588:LIM Domain Proteins; D060850:LIM-Homeodomain Proteins; D008565:Membrane Proteins; D008958:Models, Molecular; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D019906:Nuclear Magnetic Resonance, Biomolecular; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D013816:Thermodynamics; D014157:Transcription Factors
D048868:Adaptor Proteins, Signal Transducing; D030161:Avian Proteins; C114604:CRP2 protein, Coturnix japonica; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; C085473:LHX1 protein, human; D060588:LIM Domain Proteins; D060850:LIM-Homeodomain Proteins; C044539:LIM2 protein, human; D008565:Membrane Proteins; D009124:Muscle Proteins; D014157:Transcription Factors; C090114:cysteine and glycine-rich protein 3