Diimine platinum(II) and palladium(II) complexes of dithiocarbamate derivative as potential antitumor agents: synthesis, characterization, cytotoxicity, and detail dna-binding studies.
The synthesis and chemical characterization of two structurally related platinum(II) and palladium(II) complexes, [M(2,2'-bipyridine)(morpholinedithiocarbamate)]NO(3) or [M(bpy)(mor-dtc)]NO(3), where M = Pt(II) or Pd(II), are described. Studies of anti-tumor activities of these complexes against human cell tumor lines (K(562)) have been carried out. They show 50% cytotoxic concentration (Cc(50)) values much lower than that of cisplatin. Both of these water soluble complexes have been shown to interact with calf thymus DNA (ct-DNA) using difference absorption-, fluorescence-, and circular dichroism-titration techniques. These studies showed that both complexes exhibit cooperative binding and presumably intercalate in DNA. These complexes unexpectedly denature DNA at very low concentrations (50-100 microM). Several binding and thermodynamic parameters are also described.
Mansouri-Torshizi
H
H
Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran. hmtorshizi@hamoon.usb.ac.ir
Moghaddam
M I
MI
Divsalar
A
A
Saboury
A A
AA
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002942:Circular Dichroism; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D006801:Humans; D007097:Imines / Q000737:chemistry*; D007202:Indicators and Reagents / Q000378:metabolism; D020014:K562 Cells; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000970:Antineoplastic Agents; D007097:Imines; D007202:Indicators and Reagents; D009942:Organometallic Compounds; D010984:Platinum; D010165:Palladium; D004247:DNA; C427203:calf thymus DNA; D002945:Cisplatin