An anatomic gene expression atlas of the adult mouse brain.
Studying gene expression provides a powerful means of understanding structure-function relationships in the nervous system. The availability of genome-scale in situ hybridization datasets enables new possibilities for understanding brain organization based on gene expression patterns. The Anatomic Gene Expression Atlas (AGEA) is a new relational atlas revealing the genetic architecture of the adult C57Bl/6J mouse brain based on spatial correlations across expression data for thousands of genes in the Allen Brain Atlas (ABA). The AGEA includes three discovery tools for examining neuroanatomical relationships and boundaries: (1) three-dimensional expression-based correlation maps, (2) a hierarchical transcriptome-based parcellation of the brain and (3) a facility to retrieve from the ABA specific genes showing enriched expression in local correlated domains. The utility of this atlas is illustrated by analysis of genetic organization in the thalamus, striatum and cerebral cortex. The AGEA is a publicly accessible online computational tool integrated with the ABA (http://mouse.brain-map.org/agea).
Ng
Lydia
L
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Bernard
Amy
A
Lau
Chris
C
Overly
Caroline C
CC
Dong
Hong-Wei
HW
Kuan
Chihchau
C
Pathak
Sayan
S
Sunkin
Susan M
SM
Dang
Chinh
C
Bohland
Jason W
JW
Bokil
Hemant
H
Mitra
Partha P
PP
Puelles
Luis
L
Hohmann
John
J
Anderson
David J
DJ
Lein
Ed S
ES
Jones
Allan R
AR
Hawrylycz
Michael
M
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology*; D001923:Brain Chemistry / Q000235:genetics*; D001931:Brain Mapping / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D016678:Genome / Q000502:physiology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D005810:Multigene Family