Transient expression of organophosphorus hydrolase to enhance the degrading activity of tomato fruit on coumaphos.
We constructed an expression cassette of the organophosphorus pesticide degrading (opd) gene under the control of the E8 promoter. Then opd was transformed into tomato fruit using an agroinfiltration transient expression system. beta-Glucuronidase (GUS) staining, reverse transcription-polymerase chain reaction (RT-PCR), wavelength scanning, and fluorescent reaction were performed to examine the expression of the opd gene and the hydrolysis activity on coumaphos of organophosphorus hydrolase (OPH) in tomato fruit. The results show that the agroinfiltrated tomato fruit-expressed OPH had the maximum hydrolysis activity of about 11.59 U/mg total soluble protein. These results will allow us to focus on breeding transgenic plants that could not only enhance the degrading capability of fruit and but also hold no negative effects on pest control when spraying organophosphorus pesticides onto the seedlings in fields.
Zhao
Jie-hong
JH
MOE Key Laboratory of Green Pesticide and Agricultural Bioengineering; Guizhou University, Guiyang 550025, China.
Zhao
De-gang
DG
D043303:Aryldialkylphosphatase / Q000235:genetics / Q000502:physiology*; D003372:Coumaphos / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic
D007306:Insecticides; D043303:Aryldialkylphosphatase; D003372:Coumaphos