Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS).
We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods.
Heyes
Derren J
DJ
Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess St., Manchester, UKM1 7DN.
Quinn
Anne-Marie
AM
Cullis
Paul M
PM
Lee
Michael
M
Munro
Andrew W
AW
Scrutton
Nigel S
NS
D004579:Electron Transport; D007700:Kinetics; D007834:Lasers; D019001:Nitric Oxide Synthase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D011721:Pyrenes / Q000737:chemistry*
D011721:Pyrenes; C121436:thiouredopyrenetrisulfonate; D019001:Nitric Oxide Synthase