title: HHV-6 DNA throughout the tissues of two stem cell transplant patients with chromosomally integrated HHV-6 and fatal CMV pneumonitis.
abstract: Two patients with the characteristic high human herpesvirus 6 (HHV-6) DNA loads in peripheral blood caused by chromosomally integrated (CI) virus received a haematopoietic stem cell transplant (HSCT) from a donor without CI HHV-6. Both patients died in consequence of cytomegalovirus (CMV) pneumonitis. At autopsy, high amounts of CMV DNA were detected in lungs but at much lower levels in other organs. In contrast HHV-6 DNA was detected at high levels throughout the organs with the exception of donor-derived haematopoietic tissue. In individuals with chromosomal integration, HHV-6 DNA is found in every tissue of recipient origin indicating inheritance through the germ line.
authors.affiliation: Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine and Motol University Hospital, Charles University, Prague, The Czech Republic. petr.hubacek@lfmotol.cuni.cz
authors.forename: Petr
authors.initials: P
authors.lastname: Hubacek
authors.forename: Anna
authors.initials: A
authors.lastname: Virgili
authors.forename: Katherine N
authors.initials: KN
authors.lastname: Ward
authors.forename: David
authors.initials: D
authors.lastname: Pohlreich
authors.forename: Petra
authors.initials: P
authors.lastname: Keslova
authors.forename: Barbora
authors.initials: B
authors.lastname: Goldova
authors.forename: Marketa
authors.initials: M
authors.lastname: Markova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Zajac
authors.forename: Ondrej
authors.initials: O
authors.lastname: Cinek
authors.forename: Elisabeth P
authors.initials: EP
authors.lastname: Nacheva
authors.forename: Petr
authors.initials: P
authors.lastname: Sedlacek
authors.forename: Petr
authors.initials: P
authors.lastname: Cetkovsky
mesh_terms: D000328:Adult; D002648:Child; D002877:Chromosomes, Human / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000401:mortality; D004279:DNA, Viral / Q000032:analysis*; D017809:Fatal Outcome; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000401:mortality / Q000628:therapy / Q000821:virology; D008168:Lung / Q000821:virology; D008297:Male; D011014:Pneumonia / Q000401:mortality / Q000821:virology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000401:mortality / Q000628:therapy / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019349:Roseolovirus Infections / Q000235:genetics / Q000821:virology*; D016662:Virus Integration / Q000235:genetics*
chemical_list: D004279:DNA, Viral