title: Amygdala depotentiation ex vivo requires mitogen-activated protein kinases and protein synthesis.
abstract: We have recently characterized a form of ex vivo depotentiation (depotentiationex vivo), which correlates tightly with fear extinction, at thalamic input synapses onto the lateral amygdala. Here, we examined the effects of learning-attenuating drugs, reported to impair fear extinction when microinjected into the basolateral amygdala, on depotentiationex vivo. U0126, a mitogen-activated protein kinase inhibitor, and cycloheximide, a protein synthesis inhibitor, blocked depotentiationex vivo. However, ifenprodil, an NR2B-containing NMDA receptor inhibitor, did not alter depotentiationex vivo, although it blocked amygdala long-term potentiation. These findings indicate that amygdala depotentiation shares some molecular processes with learning and further suggest that different forms of synaptic plasticity in the basolateral amygdala mediate fear extinction.
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jeongyeon
authors.initials: J
authors.lastname: Kim
authors.forename: Sungmo
authors.initials: S
authors.lastname: Park
authors.forename: Sukwon
authors.initials: S
authors.lastname: Lee
authors.forename: Sukwoo
authors.initials: S
authors.lastname: Choi
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000679:Amygdala / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002070:Butadienes / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005108:Extinction, Psychological / Q000187:drug effects / Q000502:physiology; D005239:Fear / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D036881:Long-Term Synaptic Depression / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008839:Microelectrodes; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors
chemical_list: D000317:Adrenergic alpha-Antagonists; D002070:Butadienes; D004791:Enzyme Inhibitors; D009570:Nitriles; D010880:Piperidines; D011500:Protein Synthesis Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; C113580:U 0126; D003513:Cycloheximide; D020928:Mitogen-Activated Protein Kinases; C010739:ifenprodil