Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template.
Surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. In this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble UV sensitive initiator onto the surface of silica beads. The polymerization was initiated by UV radiation with N-[3-(dimethylamino)propyl]methacrylamide and N-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat of stimuli-responsive hydrogel yielded over the silica gels. The surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (K(a)) of 2.2 x 10(5)L mol(-1) and a maximum binding capacity (Q(max)) of 27.3 mgg(-1) in Tris-HCl buffer (pH 7.0). The rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3h for the previously prepared bulk imprinted hydrogel). Besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. The above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials.
Hua
Zhendong
Z
Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry & Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Zhou
Shuang
S
Zhao
Meiping
M
D000327:Adsorption; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry; D001665:Binding Sites; D015374:Biosensing Techniques; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D020100:Hydrogels; D066298:In Vitro Techniques; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide; D013499:Surface Properties; D014466:Ultraviolet Rays; D014631:Valerates / Q000737:chemistry
D001391:Azo Compounds; D020100:Hydrogels; D011506:Proteins; D014631:Valerates; C091250:4,4'-azobis(4-cyanovaleric acid); D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide