title: Effect of different models of physical exercise on oxidative stress markers in mouse liver.
abstract: The aim of this study was to investigate the effect of different protocols of physical exercise on oxidative stress markers in mouse liver. Twenty-eight male CF1 mice (30-35 g) were distributed into 4 groups (n = 7) - untrained (UT), continuous running (CR), downhill running (D-HR), and intermittent running (IR) - and underwent an 8-week training program. Forty-eight hours after the last training session, the animals were killed, and their livers were removed. Blood lactate, creatine kinase, citrate synthase, thiobarbituric acid reactive species, carbonyl, superoxide dismutase (SOD), and catalase (CAT) activities were assayed. Results show a decrease in the level of lipoperoxidation and protein carbonylation in the CR and D-HR groups. SOD activity was significantly increased and CAT activity was reduced in the CR and D-HR groups. Our findings indicate that CR and D-HR may be important for decreasing oxidative damage and in the regulation of antioxidant enzymes (SOD and CAT) in the livers of trained mice.
authors.affiliation: Universidade do Extremo Sul Catarinense-UNESC, Laboratorio de Fisiologia e Bioquimica do Exercicio, Bloco da Saude, Av Universitaria, 1105, Bairro Universitario, 88806-000, Criciuma, Santa Catarina, Brazil. luciano_acordi@yahoo.com.br
authors.forename: Luciano A
authors.initials: LA
authors.lastname: da Silva
authors.forename: Cleber A
authors.initials: CA
authors.lastname: Pinho
authors.forename: Luis G C
authors.initials: LG
authors.lastname: Rocha
authors.forename: Talita
authors.initials: T
authors.lastname: Tuon
authors.forename: Paulo C L
authors.initials: PC
authors.lastname: Silveira
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pinho
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003402:Creatine Kinase / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000502:physiology*; D018384:Oxidative Stress; D005082:Physical Exertion; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D019344:Lactic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D002950:Citrate (si)-Synthase; D003402:Creatine Kinase