The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans.
We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T(1) soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene.
Tougou
Makoto
M
National Agricultural Research Center for Tohoku Region (NARCT), Morioka, Iwate, Japan. tougou@affrc.go.jp
Yamagishi
Noriko
N
Furutani
Noriyuki
N
Kaku
Koichiro
K
Shimizu
Tsutomu
T
Takahata
Yoshihito
Y
Sakai
Jun-ichi
J
Kanematsu
Seiji
S
Hidaka
Soh
S
D000095:Acetolactate Synthase / Q000235:genetics*; D053209:Herbicide Resistance / Q000235:genetics; D006540:Herbicides / Q000494:pharmacology; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics; D010957:Plasmids; D018749:RNA, Plant / Q000235:genetics; D013025:Glycine max / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic; D019076:Transgenes
D006540:Herbicides; D010940:Plant Proteins; D018749:RNA, Plant; D000095:Acetolactate Synthase