Connexin37 mRNA expression in in vivo and in vitro mouse oocyte.
To evaluate gene expression of Connexin37 (Cx37) in oocytes from in vitro follicles at different stages, mouse preantral follicles were isolated and cultured for 12 days in vitro. Compared with in vitro follicles, follicles grown in vivo were collected at day 14 (d14), d16, d18, d20, d22 and d24 with the same stages for gene expression of Cx37 in oocytes. Our results showed that Cx37 mRNA increased along with follicular development, reached the highest level at the onset of antrum cavity formation and decreased after antrum formation in both in vivo and in vitro mouse oocytes. However, Cx37 mRNA was significant higher (p < 0.01) in in vitro cultured oocytes than in vivo oocytes. Moreover, significantly higher levels of Cx37 mRNA were found in oocytes from in vitro disrupted follicles (p < 0.01) and non-grown follicles (p < 0.05) than those from normal follicles with a similar size. These data determine temporal gene expression of Cx37 in oocytes from follicules at different stages and indicate that the gene expression level of Cx37 in oocytes could be evaluated as a criterion to the regulatory mechanism of Cx37 in an in vitro model.
Yin
Bao Ying
BY
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Zhang
Yong
Y
Sun
Jian Hong
JH
Li
Ji Xia
JX
Ma
Ye Fei
YF
D000818:Animals; D002478:Cells, Cultured; D017630:Connexins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism*; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D000097006:Gap Junction alpha-4 Protein
D017630:Connexins; D012333:RNA, Messenger; D000097006:Gap Junction alpha-4 Protein