title: Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers.
abstract: This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I) and chlorhexidine (CHX) - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.
authors.affiliation: chrispadovani@hotmail.com
authors.forename: Christiane Moreira
authors.initials: CM
authors.lastname: Padovani
authors.forename: Kazuko Uchikawa
authors.initials: KU
authors.lastname: Graziano
authors.forename: Vânia Regina
authors.initials: VR
authors.lastname: Goveia
mesh_terms: D000891:Anti-Infective Agents, Local; D002710:Chlorhexidine; D004340:Drug Contamination; D004866:Equipment Contamination; D011206:Povidone-Iodine; D012706:Serratia marcescens / Q000302:isolation & purification*
chemical_list: D000891:Anti-Infective Agents, Local; D011206:Povidone-Iodine; D002710:Chlorhexidine