Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates.
We combined high-throughput screening of differential mRNAs with mass spectrometric characterization of proteins obtained from osteocytes untreated and treated with Risedronate. Microarray analysis revealed, upon treatment, a marked upregulation of messengers encoding zinc-proteins. MS analysis identified 84 proteins in the osteocytes proteome map. Risedronate affected the expression of 10 proteins, associated with cytoskeleton, stress-response and metabolism. Data validated using gel imaging in combination with the GLaD post digestion isotopic labeling method provide the molecular basis for understanding the role of bisphosphonates as antiapoptotic drugs for osteocytes.
Bivi
Nicoletta
N
Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy, Shimadzu Research Laboratory (Europe), Manchester, UK.
Bereszczak
Jessica Z
JZ
Romanello
Milena
M
Zeef
Leo A H
LA
Delneri
Daniela
D
Quadrifoglio
Franco
F
Moro
Luigi
L
Brancia
Francesco L
FL
Tell
Gianluca
G
D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D004164:Diphosphonates / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D012968:Etidronic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020869:Gene Expression Profiling; D007553:Isotope Labeling / Q000379:methods; D010011:Osteocytes / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D000068296:Risedronic Acid; D021241:Spectrometry, Mass, Electrospray Ionization
D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D020543:Proteome; D012333:RNA, Messenger; D000068296:Risedronic Acid; D012968:Etidronic Acid