title: Prostate stromal cells produce CXCL-1, CXCL-2, CXCL-3 and IL-8 in response to epithelia-secreted IL-1.
abstract: It is well accepted that tumor microenvironment is essential for tumor cells survival, cancer progression and metastasis. However, the mechanisms by which tumor cells interact with their surrounding at early stages of cancer development are largely unidentified. The aim of this study was to identify specific molecules involved in stromal-epithelial interactions that might contribute to early stages of prostate tumor formation. Here, we show that conditioned medium (CM) from immortalized non-transformed prostate epithelial cells stimulated immortalized prostate stromal cells to express cancer-related molecules. CM obtained from epithelial cells triggered stromal cells to express and secrete CXCL-1, CXCL-2, CXCL-3 and interleukin (IL)-8 chemokines. This effect was predominantly mediated by the cytokines of the IL-1 family secreted by the epithelial cells. Thus, prostate epithelial cells induced the secretion of proinflammatory and cancer-promoting chemokines by prostate stromal cells. Such interactions might contribute to prostatic inflammation and progression at early stages of prostate cancer formation.
authors.affiliation: Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Ira
authors.initials: I
authors.lastname: Kogan-Sakin
authors.forename: Merav
authors.initials: M
authors.lastname: Cohen
authors.forename: Nicole
authors.initials: N
authors.lastname: Paland
authors.forename: Shalom
authors.initials: S
authors.lastname: Madar
authors.forename: Hilla
authors.initials: H
authors.lastname: Solomon
authors.forename: Alina
authors.initials: A
authors.lastname: Molchadsky
authors.forename: Ran
authors.initials: R
authors.lastname: Brosh
authors.forename: Yosef
authors.initials: Y
authors.lastname: Buganim
authors.forename: Naomi
authors.initials: N
authors.lastname: Goldfinger
authors.forename: Helmut
authors.initials: H
authors.lastname: Klocker
authors.forename: Jack A
authors.initials: JA
authors.lastname: Schalken
authors.forename: Varda
authors.initials: V
authors.lastname: Rotter
mesh_terms: D015153:Blotting, Western; D002450:Cell Communication; D049109:Cell Proliferation; D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000235:genetics / Q000378:metabolism*; D054426:Chemokine CXCL2 / Q000235:genetics / Q000378:metabolism*; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism
chemical_list: C475829:CXCL1 protein, human; C499706:CXCL2 protein, human; C499707:CXCL3 protein, human; D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger