title: Shotgun proteome analysis of Rhodospirillum rubrum S1H: integrating data from gel-free and gel-based peptides fractionation methods.
abstract: Beside bioreactor modeling studies, the molecular characterization of life-support organisms appeared to be essential to complete their global behavior picture, in particular, culture conditions. Using a combination of LC-MS/MS approaches with gel-free and gel-based peptides fractionation steps, we identified 932 proteins from the alpha-proteobacterium Rhodospirillum rubrum S1H. In addition, abundance data were retrieved using the recently developed emPAI approach which takes into account the number of sequenced peptides per protein. This work has also allowed identification of new and specific proteins for the Rhodospirillaceae family.
authors.affiliation: Department of Proteomics and Protein Biochemistry, University of Mons, Mons, Belgium.
authors.forename: Felice
authors.initials: F
authors.lastname: Mastroleo
authors.forename: Baptiste
authors.initials: B
authors.lastname: Leroy
authors.forename: Rob
authors.initials: R
authors.lastname: Van Houdt
authors.forename: Catherine
authors.initials: C
authors.lastname: s' Heeren
authors.forename: Max
authors.initials: M
authors.lastname: Mergeay
authors.forename: Larissa
authors.initials: L
authors.lastname: Hendrickx
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012244:Rhodospirillaceae / Q000145:classification / Q000378:metabolism; D012247:Rhodospirillum rubrum / Q000378:metabolism*; D013045:Species Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome