Effects of macronutrient additions on nickel uptake and distribution in the dinoflagellate Prorocentrum donghaiense Lu.
The influences of macronutrient additions on nickel (Ni) uptake and distribution in the subcellular structures and macromolecular components of the dinoflagellate Prorocentrum donghaiense Lu were examined using a radioisotope tracer method. The results showed that nitrate addition enhanced the uptake of Ni by P. donghaiense, whereas phosphate addition inhibited Ni uptake at high-Ni concentration. Nitrate or phosphate addition significantly affected Ni distribution in the subcellular structures and components. The majority of Ni was found in the soluble substances (>70%) and in the proteins (55.0-79.6%) of the algal cells. Urea reduced the Ni content in the amino acid-carbohydrate but elevated its content in proteins, and shown significantly correlated with the protein content of the algal cells. Thus, nutrient enrichment could influence both metal uptake and its distribution in the subcellular structures and components of the phytoplankton, as well as its subsequent transfer in marine food chains.
Hong
Hua-Sheng
HS
State Key Laboratory of Marine Environmental Science, Xiamen University/Environmental Science Research Center, Xiamen University, No. 192, Daxue Road, Siming Zone, Xiamen 361005, People's Republic of China.
Wang
Ming-Hua
MH
Huang
Xu-Guang
XG
Wang
Da-Zhi
DZ
D020418:Algal Proteins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D004141:Dinoflagellida / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004463:Ecology / Q000379:methods; D005068:Eutrophication; D009532:Nickel / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism* / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism* / Q000494:pharmacology; D014508:Urea / Q000378:metabolism / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000494:pharmacology
D020418:Algal Proteins; D009566:Nitrates; D010710:Phosphates; D014874:Water Pollutants, Chemical; D009532:Nickel; D014508:Urea