Osmoregulated periplasmic glucans are needed for competitive growth and biofilm formation by Salmonella enterica serovar Typhimurium in leafy-green vegetable wash waters and colonization in mice.
Osmoregulated periplasmic glucans (OPGs) are major periplasmic constituents of Gram-negative bacteria. The role of OPGs has been postulated in symbiotic as well as pathogenic host-microorganism interactions. Here, we report the role of OPGs from Salmonella enterica serovar Typhimurium during growth and biofilm formation in leafy-green vegetable wash water. The opgGH mutant strain, which was defective in OPG biosynthesis, initiated the growth at a slower rate in wash waters obtained from spinach, lettuce and green collard and severely impaired biofilm formation. The lack of OPG synthesis did not influence biofilm formation by the opgGH mutant in low-nutrient low-osmolarity laboratory media. In coculture experiments initiated with equal proportions of cells, the opgGH mutant was outnumbered by the wild-type strain under the planktonic as well as the biofilm growth conditions. The opgGH mutant strain poorly colonized mouse organs when introduced orally along with the wild-type strain. This is the first report demonstrating the role of OPGs of Salmonella in competitive colonization of biofilms, planktonic cultures and mouse organs.
Liu
Liu
L
Produce Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705-2350, USA.
Tan
Shawn
S
Jun
Won
W
Smith
Allen
A
Meng
Jianghong
J
Bhagwat
Arvind A
AA
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D017353:Gene Deletion; D005936:Glucans / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence; D014871:Water Microbiology; D014882:Water-Electrolyte Balance
D001426:Bacterial Proteins; D005936:Glucans; D011135:Polysaccharides, Bacterial