Path dependence of three-phase or two-phase end points in fluid binary lipid mixtures.
The phase behavior of anionic/zwitterionic mixtures can be controlled by tuning the charge state of the anionic lipid. In the case of dioleoylphosphatidic acid (DOPA)/dioleoylphosphatidylcholine (DOPC) mixtures, demixing occurs either when DOPA is protonated or when DOPA(2-):Ca(2+) complexes form. Herein it will be shown that the final end point, a three-phase or two-phase system, depends on the order in which the charge state is manipulated. The facile accessibility of different end points is a clear demonstration of the inherent flexibility of biological systems.
Lamberson
Emily R
ER
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Cambrea
Lee R
LR
Rochet
Jean-Christophe
JC
Hovis
Jennifer S
JS
D000465:Algorithms; D002118:Calcium / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D010712:Phosphatidic Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry
D008051:Lipid Bilayers; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; C037657:dioleoylphosphatidic acid; C017251:1,2-oleoylphosphatidylcholine; D002118:Calcium