title: Oral administration of pharmacologically active substances to squid: a methodological description.
abstract: The squid giant synapse is a well-defined experimental preparation for the study of ligand-dependant synaptic transmission. Its large size gives direct experimental access to both presynaptic and postsynaptic junctional elements, allowing direct optical, biophysical, and electrophysiological analysis of depolarization-release coupling. However, this important model has not been utilized in pharmacological studies, other than those implementable acutely in the in vitro condition. A method is presented for oral administration of bioactive substances to living squid. Electrophysiological characterization and direct determination of drug absorption into the nervous system demonstrate the administration method described here to be appropriate for pharmacological research.
authors.affiliation: Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA.
authors.forename: William
authors.initials: W
authors.lastname: Berk
authors.forename: Jake
authors.initials: J
authors.lastname: Teperman
authors.forename: Kerry D
authors.initials: KD
authors.lastname: Walton
authors.forename: Kazunari
authors.initials: K
authors.lastname: Hirata
authors.forename: Mutsuyuki
authors.initials: M
authors.lastname: Sugimori
authors.forename: Rodolfo R
authors.initials: RR
authors.lastname: Llinas
mesh_terms: D000284:Administration, Oral; D000818:Animals; D049832:Decapodiformes / Q000502:physiology*; D005260:Female; D009431:Neural Conduction; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D010880:Piperidines / Q000633:toxicity; D011720:Pyrazoles / Q000633:toxicity; D013569:Synapses
chemical_list: C524478:1,3-bis(4-hydroxyphenyl)-4-methyl-5-(4-(2-piperidinylethoxy)phenol)-1H-pyrazole; D004364:Pharmaceutical Preparations; D010880:Piperidines; D011720:Pyrazoles