title: Diffuse reflectance spectroscopy detects increased hemoglobin concentration and decreased oxygenation during colon carcinogenesis from normal to malignant tumors.
abstract: Blood content and tumor oxygen level are important biomarkers and prognostic indicators in patients with colorectal cancer (CRC). However, noninvasive measurements of both quantities in human colon are limited. In this study, we extracted the total hemoglobin concentration (THC) and oxygen saturation (StO(2)) of normal, premalignant, and malignant colonic tissues in 27 patients using a diffuse reflectance instrument and algorithms based on the diffusion equation. The mean+/-standard error of THC and StO(2) from all normal sites (n=26) is 93.4+/-17.1microM and 67.2+/-3.7%, respectively. THC increased to 136.9+/-23.8microM and 153.8+/-38.6microM and StO(2) decreased to 51.3+/-7.0% and 26.4+/-6.1% for premalignant and malignant tissues, respectively. The disease-to-normal THC ratios are 3.2+/-1.1 and 4.4+/-1.9 and the disease-to-normal StO(2) ratios are 0.7+/-0.1 and 0.5+/-0.1 for pr alignant and malignant tissues, respectively. These results demonstrate the feasibility of a robust optical method to assess colon THC and StO2 at all stages of carcinogenesis in vivo so that the angiogenesis and hypoxia of the disease and the therapeutic role can be studied in CRC patients.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan. hwwang2@ym.edu.tw
authors.forename: Hsing-Wen
authors.initials: HW
authors.lastname: Wang
authors.forename: Jeng-Kai
authors.initials: JK
authors.lastname: Jiang
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Lin
authors.forename: Jen-Kou
authors.initials: JK
authors.lastname: Lin
authors.forename: Guang-Jie
authors.initials: GJ
authors.lastname: Huang
authors.forename: Jia-Sin
authors.initials: JS
authors.lastname: Yu
mesh_terms: D000465:Algorithms; D014408:Biomarkers, Tumor / Q000032:analysis*; D003106:Colon / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000175:diagnosis*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D010100:Oxygen / Q000032:analysis*; D011230:Precancerous Conditions / Q000737:chemistry / Q000175:diagnosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D006454:Hemoglobins; D010100:Oxygen