title: Detection and size analysis of proteins with switchable DNA layers.
abstract: We introduce a chip-compatible scheme for the label-free detection of proteins in real-time that is based on the electrically driven conformation switching of DNA oligonucleotides on metal surfaces. The switching behavior is a sensitive indicator for the specific recognition of IgG antibodies and antibody fragments, which can be detected in quantities of less than 10(-18) mol on the sensor surface. Moreover, we show how the dynamics of the induced molecular motion can be monitored by measuring the high-frequency switching response. When proteins bind to the layer, the increase in hydrodynamic drag slows the switching dynamics, which allows us to determine the size of the captured proteins. We demonstrate the identification of different antibody fragments by means of their kinetic fingerprint. The switchDNA method represents a generic approach to simultaneously detect and size target molecules using a single analytical platform.
authors.affiliation: Walter Schottky Institut, Technische Universitat Munchen, Garching, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Rant
authors.forename: Erika
authors.initials: E
authors.lastname: Pringsheim
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kaiser
authors.forename: Kenji
authors.initials: K
authors.lastname: Arinaga
authors.forename: Jelena
authors.initials: J
authors.lastname: Knezevic
authors.forename: Marc
authors.initials: M
authors.lastname: Tornow
authors.forename: Shozo
authors.initials: S
authors.lastname: Fujita
authors.forename: Naoki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Gerhard
authors.initials: G
authors.lastname: Abstreiter
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D005453:Fluorescence; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D004247:DNA