Label-free relative quantitation of prokaryotic proteomes using the accurate mass and time tag approach.
Prokaryotic protein expression changes in detectable amounts due to the environmental stimuli encountered by the organism. To understand the underlying mechanisms involved it is necessary to comprehensively detect both the proteins present and their relative abundance under the growth conditions of interest. LC-MS based accurate mass and time (AMT) tag method along with the use of clustering software can provide a visual and more comprehensive understanding of significant protein abundance increases and decreases. These data then can be effectively used to pin-point proteins of interest for further genetic and physiological studies. This method allows for the identification and quantitation of thousands of proteins in a single mass spectrometric analysis and is more comprehensive than two dimensional electrophoresis and shotgun approaches.
Hixson
Kim K
KK
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
D000478:Alkylation; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002457:Cell Extracts / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011387:Prokaryotic Cells / Q000378:metabolism*; D011489:Protein Denaturation; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D011804:Quinolines / Q000737:chemistry; D011817:Rabbits; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry; D012680:Sensitivity and Specificity; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D001426:Bacterial Proteins; D002457:Cell Extracts; D008565:Membrane Proteins; D010455:Peptides; D020543:Proteome; D011804:Quinolines; D012394:Rosaniline Dyes; C024795:bicinchoninate reagent; C048139:Coomassie blue; D014357:Trypsin