title: Kazachstania wufongensis sp. nov., an ascosporogenous yeast isolated from soil in Taiwan.
abstract: A new yeast species, Kazachstania wufongensis, is proposed in this paper based on six strains isolated from soil in Taiwan. The species may produce one to four ellipsoidal ascospores in each ascus, directly transformed from diploid cells. Genus assignment and distinction of the species from other recognized species of Kazachstania is based on morphological and physiological characteristics, and on phylogenetic analysis of nucleotide sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene. Sequence analysis of the D1/D2 domains of the LSU rRNA gene reveals that K. wufongensis is a member of the Kazachstania exigua complex, and its phylogenetically closest relatives are K. exigua, K. barnettii, K. bulderi, and K. turicensis. The species can be further differentiated from the other phylogenetically related species based on internal transcribed spacer sequence and electrophoretic karyotype. Therefore, the new species Kazachstania wufongensis sp. nov. is proposed. The type strain of this new species, which was isolated from forest soil in Wufong, Hsinchu, Taiwan, is FN21S03(T) (=CBS 10886(T) = BCRC 23138(T)).
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, Taiwan. leecf@mail.nhcue.edu.tw
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
authors.forename: Cheng-Hsu
authors.initials: CH
authors.lastname: Yao
authors.forename: Yi-Ru
authors.initials: YR
authors.lastname: Liu
authors.forename: Shuh-Sen
authors.initials: SS
authors.lastname: Young
authors.forename: Ku-Shang
authors.initials: KS
authors.lastname: Chang
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D007621:Karyotyping; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000166:cytology; D013624:Taiwan
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S