The bioactivity of rhBMP-2 immobilized poly(lactide-co-glycolide) scaffolds.
In this study, immobilization of rhBMP-2 on polylactone-type polymer scaffolds via plasma treatment was investigated. To introduce proper functional groups on the surface of poly(lactide-co-glycolide) (PLGA) matrix, PLGA films were treated under different atmospheres, such as oxygen, ammonia and carbon dioxide, respectively, and then incubated in rhBMP-2 solution of de-ionized water. The effect of various plasma-treated PLGA films on binding rhBMP-2 was investigated and compared. It was found that PLGA binding ability to rhBMP-2 was enhanced by carbon dioxide and oxygen plasma treatment, and the binding ability of the oxygen plasma-treated PLGA (OT-PLGA) to rhBMP-2 was the strongest after oxygen plasma treating for 10 min under a power of 50 W. The changes of surface chemistry and surface topography of PLGA matrix induced by oxygen plasma treatment played main roles in improving the PLGA binding ability to rhBMP-2. The stability of rhBMP-2 bound on OT-PLGA film was determined under a dynamic condition by a Parallel Plate Flow Chamber. The result showed that the rhBMP-2 had been immobilized on the OT-PLGA film. Mouse OCT-1 osteoblast-like cell as a model cell was cultured on the rhBMP-2 bound OT-PLGA (OT-PLGA/BMP) in vitro, which showed that the bound rhBMP-2 via oxygen plasma treatment was bioactive. Depending on hydrophilicity and rich polar O-containing groups of the OT-PLGA scaffold, different amount of rhBMP-2 could be evenly immobilized on the surface of the OT-PLGA scaffold. The immobilized rhBMP-2 had stimulated differentiation of OCT-1 cell and accelerated process of mineralization of OCT-1 cell in the scaffold. It revealed the rhBMP-2 immobilized PLGA scaffold had good cell affinity.
Shen
Hong
H
BNLMS, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Hu
Xixue
X
Yang
Fei
F
Bei
Jianzhong
J
Wang
Shenguo
S
D000469:Alkaline Phosphatase / Q000032:analysis; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000494:pharmacology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000166:cytology; D005740:Gases; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D010100:Oxygen / Q000494:pharmacology; D011098:Polyglactin 910 / Q000737:chemistry / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D054457:Tissue Scaffolds
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D005740:Gases; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D011098:Polyglactin 910; D000641:Ammonia; D000469:Alkaline Phosphatase; D010100:Oxygen