title: Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli.
abstract: Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 wa scloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This allergen was purified by immobilized Ni2+-affinity chromatography. The purified Pen b 26 was characterized by immunological, biochemical and biophysical methods. C-His6 tagged Pen b 26 produced several fold greater yield than N-His6 tagged Pen b 26. The affinity of C-His6 tagged Pen b 26 for the specific antibody was also 2.75 times higher than N-His6 tagged Pen b 26
authors.affiliation: Healthy Environments and Consumer Safety Branch, Health Canada, 251 F.G. Banting Driveway, Rm C229, Tunney's Pasture, Postal Locator 2202D2, Ottawa, Ont. K1A0K9, Canada. serdal_sevinc@hc-sc.gc.ca
authors.forename: M Serdal
authors.initials: MS
authors.lastname: Sevinc
authors.forename: Veena
authors.initials: V
authors.lastname: Kumar
authors.forename: Makonnen
authors.initials: M
authors.lastname: Abebe
authors.forename: Susantha
authors.initials: S
authors.lastname: Mohottalage
authors.forename: Premkumari
authors.initials: P
authors.lastname: Kumarathasan
authors.forename: Renaud
authors.initials: R
authors.lastname: Vincent
authors.forename: Hari M
authors.initials: HM
authors.lastname: Vijay
mesh_terms: D000485:Allergens / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001249:Asthma / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D015870:Gene Expression; D010407:Penicillium / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D005656:Fungal Proteins; D011994:Recombinant Proteins