title: [MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line].
abstract: OBJECTIVE
  To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).
  
  
  METHODS
  The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.
  
  
  RESULTS
  5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.
  
  
  CONCLUSIONS
  MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.
authors.affiliation: Department of Pharmacy, People's Hospital of Hainan Province, Haikou 570311, China. hfx1223@163.com
authors.forename: Fang-xuan
authors.initials: FX
authors.lastname: Han
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Ling
authors.initials: L
authors.lastname: Ru
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D013778:Tetrazolium Salts
chemical_list: D000964:Antimetabolites, Antineoplastic; D004396:Coloring Agents; D013778:Tetrazolium Salts; D005472:Fluorouracil