Extraction of erythrocyte enzymes for the preparation of polyhemoglobin-catalase-superoxide dismutase.
In sustained severe ischemia, reperfusion with oxygen carriers may result in ischemia-reperfusion injuries because of the release of damaging oxygen radicals. A nanobiotechnology-based polyhemogloin-calatase-superoxide dismutase can prevent this because the oxygen carrier, polyhemoglobin, is linked to antioxidant enzymes, catalase and superoxide dismutase. However, these antioxidant enzymes come from nonhuman sources and recombinant human enzymes are expensive. This paper describes our study on extracting these enzymes from red blood cells and analyzing the amount of enzymes needed for adequate protection from ischemia-reperfusion.
Gu
Jingsong
J
Artificial Cells & Organs Research Centre, Department of Physiology, McGill University, Montreal, Quebec, Canada.
Chang
Thomas Ming Swi
TM
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism; D002374:Catalase / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002457:Cell Extracts; D019610:Cytoprotection; D004912:Erythrocytes / Q000201:enzymology*; D006454:Hemoglobins / Q000302:isolation & purification / Q000378:metabolism*; D036103:Nanotechnology; D017382:Reactive Oxygen Species; D015427:Reperfusion Injury / Q000097:blood / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000378:metabolism*
D000975:Antioxidants; D002457:Cell Extracts; D006454:Hemoglobins; D017382:Reactive Oxygen Species; C037158:polyhemoglobin; D002374:Catalase; D013482:Superoxide Dismutase