title: Influence of abrasion on biofilm detachment: evidence for stratification of the biofilm.
abstract: The objective of this paper was to understand the detachment of multispecies biofilm caused by abrasion. By submitting a biofilm to different abrasion strengths (collision of particles), stratification of biofilm cohesion could be highlighted and related to stratification of biofilm bacterial communities using the PCR-SSCP fingerprint method. The biofilm comprised a thick top layer, weakly cohesive and composed of one dominant species, and a thin basal layer, strongly cohesive and composed of a more diverse population. These observations suggest that microbial composition of biofilms may be an important parameter in understanding biofilm detachment.
authors.affiliation: INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Rochex
authors.forename: Anthony
authors.initials: A
authors.lastname: Massé
authors.forename: Renaud
authors.initials: R
authors.lastname: Escudié
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bernet
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000254:growth & development*; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors; D001709:Biotechnology; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D004867:Equipment Design; D008863:Microspheres; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011126:Polypropylenes; D013314:Stress, Mechanical
chemical_list: D004269:DNA, Bacterial; D011126:Polypropylenes