title: A proteomic analysis of powdery mildew (Blumeria graminis f.sp. hordei) conidiospores.
abstract: Conidiospores are the asexual propagation units of many plant-pathogenic fungi. In this article, we report an annotated proteome map of ungerminated conidiospores of the ascomycete barley powdery mildew pathogen, Blumeria graminis f.sp. hordei. Using a combination of two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, we have identified the proteins in 180 spots, which probably represent at least 123 distinct fungal gene products. Most of the identified proteins have a predicted function in carbohydrate, lipid or protein metabolism, indicating that the spore is equipped for the catabolism of storage compounds as well as for protein biosynthesis and folding on germination.
authors.affiliation: Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Noir
authors.forename: Thomas
authors.initials: T
authors.lastname: Colby
authors.forename: Anne
authors.initials: A
authors.lastname: Harzen
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schmidt
authors.forename: Ralph
authors.initials: R
authors.lastname: Panstruga
mesh_terms: D001203:Ascomycota / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000032:analysis; D010455:Peptides / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D020033:Protein Isoforms / Q000378:metabolism; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D013172:Spores, Fungal / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D005656:Fungal Proteins; D010455:Peptides; D020033:Protein Isoforms; D020543:Proteome