Influence of high temperature on cadmium-induced skeletal deformities in juvenile mosquitofish (Gambusia affinis).
The aim of this study was to assess the effect of high temperature on cadmium (Cd)-induced skeletal deformities in juvenile Mosquitofish, Gambusia affinis. For this purpose, 188 juveniles (1 day old) were equally divided into the control group, which was maintained in Cd-free water at 24 degrees C, and three treated groups exposed either to Cd (0.4 mg/l as Cd Cl(2)) at 24 degrees C, to high temperature (32 degrees C), or to Cd at 32 degrees C for 30 days. The results showed that Cd exposure at 24 degrees C significantly increased the Cd accumulation (P < 0.0001) in the whole tissues of juveniles as well as the incidence of skeletal deformities (P < 0.01) compared with control animals. Exposure to high temperature also led to a significant increase in the incidence of skeletal deformities (P < 0.01) with respect to the control group. Interestingly, our results showed that the combined exposure to Cd and high temperature led to a more significant increase in Cd accumulation and in the frequency of spinal deformities than exposure to Cd or high temperature alone. These results confirm that temperature increases Cd toxicity and needs to be taken into account for the accurate prediction and assessment of Cd-induced spinal deformities in fish.
Sassi
Asma
A
UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000, Monastir, Tunisia.
Annabi
Ali
A
UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000, Monastir, Tunisia.
Kessabi
Kaouthar
K
UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000, Monastir, Tunisia.
Kerkeni
Abdelhamid
A
Eléments Trace, Radicaux Libres, Systèmes Antioxydants et Pathologies Humaines et Environnement, Faculté de Médecine, 5000, Monastir, Tunisia.
Saïd
Khaled
K
UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000, Monastir, Tunisia.
Messaoudi
Imed
I
UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000, Monastir, Tunisia. imed_messaoudi@yahoo.fr.
D000423:Alcian Blue; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D000880:Anthraquinones; D002104:Cadmium / Q000493:pharmacokinetics* / Q000633:toxicity*; D003532:Cyprinodontiformes; D013054:Spectrophotometry, Atomic; D013131:Spine / Q000002:abnormalities*; D013696:Temperature; D014416:Tunisia
D000880:Anthraquinones; D002104:Cadmium; C004468:Alizarin Red S; D000423:Alcian Blue