[CD133(+) cells derived from human placenta identified as initiating cells for LTC-IC colony formation].
The aim of this study was to evaluate whether human placenta CD133(+) cells have an ability to reconstitute long-term hematopoiesis. Magnetic-activated cell sorting (MACS) was applied to enrich human placental CD133(+) cells. The isolated human placental CD133(+)cells of four different densities were established by limiting-dilution assay and primary fetal bone marrow stromal cells separated from bone marrow as feeder layer cells were co-cultured in long-term culture system so as to observe the incidence of long-term culture initiating-cells (LTC-IC) and their ability of proliferation and differentiation.The results showed that human placenta derived CD133(+) cells contained LTC-IC with frequency of 1/645 which have an ability to proliferate and differentiate into granulocyte/macrophage colony-forming units (CFU-GM) and mixed colony-forming units (CFU-Mix). In all LTC-IC positive wells, 71.43% form only CFU-GM and 28.57% display both CFU-GM and CFU-Mix formation. It is concluded that human placental CD133(+) cells possess LTC-IC with colony-forming capacity of hematopoietic early progenitor cells.
Liu
Hong-Lin
HL
The Affiliated Hospital, Zunyi Medical College, Zunyi 563003, Guizhou Province, China.
Chen
Dai-Xiong
DX
Fang
Ning
N
Zhang
Tao
T
Liu
Zu-Lin
ZL
Liu
Jin-Wei
JW
Wan
Wei-Hong
WH
Qi
Ying
Y
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D010455:Peptides / Q000276:immunology*; D010920:Placenta / Q000166:cytology* / Q000276:immunology; D011247:Pregnancy
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides