Detection of antigens reactive to IgE and IgA during wheat seed maturation and in different wheat cultivars.
BACKGROUND
Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease.


OBJECTIVE
To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars.


METHODS
Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease.


RESULTS
With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable.


CONCLUSIONS
The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.
Constantin
Claudia
C
Division of Immunopathology, Department of Pathophysiology, Center of Physiology, Pathophysiology and Immunology, Medical University of Vienna, Vienna, Austria.
Touraev
Alisher
A
Heberle-Bors
Erwin
E
Quirce
Santiago
S
Huber
Wolf Dietrich
WD
Valenta
Rudolf
R
D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology; D052179:Antigens, Plant / Q000032:analysis / Q000276:immunology*; D002446:Celiac Disease / Q000276:immunology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000276:immunology; D055314:Seed Storage Proteins / Q000276:immunology; D012639:Seeds / Q000254:growth & development / Q000276:immunology*; D014908:Triticum / Q000276:immunology*; D021182:Wheat Hypersensitivity / Q000097:blood / Q000276:immunology*
D000485:Allergens; D052179:Antigens, Plant; D007070:Immunoglobulin A; D010936:Plant Extracts; D055314:Seed Storage Proteins; D007073:Immunoglobulin E