Determination of azole antifungal medicines using zero-order and derivative UV spectrophotometry.
This paper presents a new methodology of quantitative determination of seven azole antifungal medicines widely used in therapy. Analyses were performed directly by using zero-order (fluconazole), first derivative (bifonazole, clotrimazole, econazole, itraconazole, miconazole) and second derivative (ketoconazole) UV spectrophotometry. Validation of all methods confirms their proper precision (%RSD = 0.47-2.86), recovery (98.7-101.4) and linearity (r coefficient over 0,999) in concentrations under investigation. The parameters received enable the developed procedure to be used in quantitative and as auxiliary in qualitative pharmaceutical analysis.
Ekiert
Radosław J
RJ
Department of Inorganic and Analytical Chemistry, Collegium Medicum, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland.
Krzek
Jan
J
D000935:Antifungal Agents / Q000032:analysis*; D001393:Azoles / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000935:Antifungal Agents; D001393:Azoles