Effect of the proton motive force inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development.
OBJECTIVE
Proton motive force (PMF) inhibition enhances the intracellular accumulation of autoinducers possibly interfering with biofilm formation. We evaluated the effect of the PMF inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development.


RESULTS
Four epidemiologically unrelated P. aeruginosa isolates were studied. A MexAB-oprM overproducing strain was used as control. Expression of gene mexB was examined and biofilm formation after incubation with 0, 125 and 25 micromol l(-1) of CCCP was investigated. Mean values of optical density were analysed with one-way analysis of variance and t-test. Two isolates subexpressed mexB gene and only 25 micromol l(-1) of CCCP affected biofilm formation. Biofilms of the other two isolates and control strain PA140 exhibited significantly lower absorbance (P ranging from < 001 to < 0.05) with either 12.5 or 25 micromol l(-1) of CCCP.


CONCLUSIONS
The PMF inhibitor CCCP effect was correlated with the expression of MexAB-OprM efflux system and found to compromise biofilm formation in P. aeruginosa.


CONCLUSIONS
These data suggest that inhibition of PMF-dependent trasporters might decrease biofilm formation in P. aeruginosa.
Ikonomidis
A
A
Department of Microbiology, University Hospital of Larissa, Larissa, Greece. ado@med.uth.gr
Tsakris
A
A
Kanellopoulou
M
M
Maniatis
A N
AN
Pournaras
S
S
D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000187:drug effects*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006801:Humans; D018892:Proton-Motive Force; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development*; D014475:Uncoupling Agents / Q000494:pharmacology*
D001426:Bacterial Proteins; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone