Synthesis, characterization, and bioconjugation of fluorescent gold nanoclusters toward biological labeling applications.
Synthesis of ultrasmall water-soluble fluorescent gold nanoclusters is reported. The clusters have a decent quantum yield, high colloidal stability, and can be readily conjugated with biological molecules. Specific staining of cells and nonspecific uptake by living cells is demonstrated.
Lin
Cheng-An J
CA
Department of Biomedical Engineering, Chung Yuan Christian University, Chung-Li 32023, Taiwan, ROC.
Yang
Ting-Ya
TY
Lee
Chih-Hsien
CH
Huang
Sherry H
SH
Sperling
Ralph A
RA
Zanella
Marco
M
Li
Jimmy K
JK
Shen
Ji-Lin
JL
Wang
Hsueh-Hsiao
HH
Yeh
Hung-I
HI
Parak
Wolfgang J
WJ
Chang
Walter H
WH
D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival; D002850:Chromatography, Gel; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling / Q000379:methods*; D013379:Substrate Specificity
D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006046:Gold