title: 7-Difluoromethyl-5,4'-dimethoxygenistein inhibits oxidative stress induced adhesion between endothelial cells and monocytes via NF-kappaB.
abstract: 7-Difluoromethyl-5,4'-dimethoxygenistein (dFMGEN), prepared by the difluoromethylation and alkylation of genistein, is an active new chemical entity. The effects of dFMGEN on cell adhesion and inflammation were investigated in human umbilical vein endothelial cells, and whether its role involved regulation of NF-kappaB expression was also studied. Results demonstrated that pretreatment with dFMGEN decreased the adhesion between vascular endothelial cells and monocytes, reduced the release of E-Selectin, ICAM-1, IL-6 and TNF-alpha in vascular endothelial cells, and down-regulated the expression of NF-kappaB. Therefore, data suggested that dFMGEN can effectually inhibit the adhesion between vascular endothelial cells and monocytes induced by oxidative stress and inflammatory reaction, and its role is connected with the downregulation of NF-kappaB.
authors.affiliation: College of Medicine, University of South China, Hengyang, Hunan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Zheng
authors.forename: Hong-lin
authors.initials: HL
authors.lastname: Xiang
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Fu
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Cao
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D019040:E-Selectin / Q000187:drug effects / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D019833:Genistein / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
chemical_list: C541079:7-difluoromethyl-5,4'-dimethoxygenistein; D019040:E-Selectin; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D019833:Genistein