A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation.
Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
Morley
Rosalind H
RH
Department of Physiology, Development and Neuroscience, Cambridge University, Anatomy Building, Downing Street, Cambridge, CB2 3DY, United Kingdom.
Lachani
Kim
K
Keefe
Damian
D
Gilchrist
Michael J
MJ
Flicek
Paul
P
Smith
James C
JC
Wardle
Fiona C
FC
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D019521:Body Patterning / Q000235:genetics; D019070:Cell Lineage; D017124:Conserved Sequence; D005326:Fetal Proteins / Q000235:genetics / Q000378:metabolism*; D054262:Gastrulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008648:Mesoderm / Q000196:embryology*; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology; D011485:Protein Binding; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism; D000098908:Brachyury Protein
D005326:Fetal Proteins; D018398:Homeodomain Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; D000098908:Brachyury Protein; C096660:noto protein, zebrafish