title: Polymorphonuclear neutrophil response to hydroxyapatite particles, implication in acute inflammatory reaction.
abstract: Hydroxyapatite (HA) is widely used as a bone substitute or coating biomaterial in bone diseases or prosthesis metal parts. The release of HA particles induces an inflammatory response and, if uncontrolled, could result in implant loss. Among the hallmarks of such inflammatory response is early recruitment of the polymorphonuclear cells (PMNs). The purpose of this work is to investigate the response of PMNs following exposure to HA in terms of secreted mediators. Our study shows that HA particles increase the release of pro-inflammatory mediators such as interleukin-1alpha, as well as chemotactic factors such as interleukin-8, macrophage inflammatory protein-1alpha and macrophage inflammatory protein-1beta. HA also induces an increase in matrix metalloproteinase 9 expression. Taken together, our data demonstrate for the first time that HA is capable of activating PMNs, a phenomenon that could potentially contribute to the onset of implant-associated inflammation.
authors.affiliation: INSERM, UMR-S 926, CHU de Reims, IFR 53, URCA, 1 avenue du Maréchal Juin, 51095 Reims, Cedex, France. frederic.velard@etudiant.univ-reims.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Velard
authors.forename: Dominique
authors.initials: D
authors.lastname: Laurent-Maquin
authors.forename: Christine
authors.initials: C
authors.lastname: Guillaume
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bouthors
authors.forename: Edouard
authors.initials: E
authors.lastname: Jallot
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Nedelec
authors.forename: Abderrazzaq
authors.initials: A
authors.lastname: Belaaouaj
authors.forename: Patrice
authors.initials: P
authors.lastname: Laquerriere
mesh_terms: D048430:Cell Shape / Q000187:drug effects; D002630:Chemotactic Factors / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000201:enzymology / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008954:Models, Biological; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000187:drug effects
chemical_list: D002630:Chemotactic Factors; D016207:Cytokines; D018836:Inflammation Mediators; D016209:Interleukin-8; D017886:Durapatite; D020780:Matrix Metalloproteinase 9