Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon.
The secretion of colorectal epithelium is important for maintaining the physiological function of colorectal organ. Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. Polarity is essential for directed secretion of substances produced by epithelial cells to the external (luminal) compartment; CAS overexpression induced polarization of HT-29 cells. CAS was punctate stained in the cytoplasm of HT-29 cells, and CAS overexpression increased the translocation of CAS-stained vesicles to the cytoplasm near cell membrane and cell protrusions. CAS overexpression increased the secretion of carcinoembryonic antigen (CEA) and cathepsin D. Immunohistochemistry showed CAS was positively stained in the goblet cells of colon mucosa and cells in the crypts of Lieberkühn of human colon as well as the glands in metastatic colorectal cancer tissue. Our results suggest that CAS regulates the secretion of colorectal cells and may regulate the metastasis of colorectal cancer.
Tsao
Tang-Yi
TY
Department of Pathology, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.
Tsai
Chin-Shaw Stella
CS
Tung
Jai-Nien
JN
Chen
Shun-Liang
SL
Yue
Chia-Herng
CH
Liao
Ching-Fong
CF
Wang
Chi-Chao
CC
Jiang
Ming-Chung
MC
D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D029563:Cellular Apoptosis Susceptibility Protein / Q000378:metabolism*; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry
D002272:Carcinoembryonic Antigen; D029563:Cellular Apoptosis Susceptibility Protein; D002402:Cathepsin D