Determination of the metal-dithiolate fold angle in mononuclear molybdenum(V) centers by EPR spectroscopy.
The active sites of mononuclear molybdenum-containing enzymes contain a low-symmetry Mo(V)-dithiolene intermediate whose structure can be probed using electron paramagnetic resonance (EPR). The relationship between experimental EPR spectra and the electronic and geometric structure of the active site can be difficult to establish, not least because of the low molecular symmetry. When density functional theory is used, it is possible to assess this relationship by systematically varying the geometric structure and comparing the theoretical EPR parameters with those obtained experimentally. We employed this approach to examine the relationship between the metal-dithiolate fold angle and the monoclinic spin Hamiltonian parameters (g, A, beta) of a prototypical mononuclear molybdenyl model complex. By comparing the experimental EPR parameters with these results, we show that the metal-dithiolate fold angle of the complex in solution may be obtained from the non-coincidence angle beta that transforms the principal axes of g to those of A. This will provide a useful method for probing the structure of the Mo(V) intermediate of mononuclear molybdenum enzymes, where the electronic structure of the active site is modulated by the fold angle of the dithiolate ligand (the "metal-dithiolate folding effect").
Drew
Simon C
SC
The University of Queensland, Centre for Magnetic Resonance and Centre for Metals in Biology, Queensland 4072, Australia.
Hanson
Graeme R
GR
D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D008670:Metals / Q000737:chemistry*; D008982:Molybdenum / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D011789:Quantum Theory; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D008670:Metals; D013438:Sulfhydryl Compounds; D008982:Molybdenum; D010088:Oxidoreductases