title: Life cycle of Rickettsia slovaca in L929 cell line studied by quantitative real-time PCR and transmission electron microscopy.
abstract: Rickettsia slovaca, a member of spotted fever rickettsiae, is an agent of a mild human disease known as Tibola or Debonel. Using quantitative real-time PCR we identified the highest point of multiplication of wild and standard type (strain B) of R. slovaca on the second vs. the fourth day postinfection. Comparing both types of R. slovaca by transmission electron microscopy substantiated different cytopathological and morphological changes in infected cells and other differences, for example a slight shift of stages during the life cycle that presented in a variety of forms and localization of the studied Rickettsia within the infected cells. Our study provides a valuable insight into the pathogenicity and virulence of R. slovaca.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Boldis
authors.forename: Jasna
authors.initials: J
authors.lastname: Strus
authors.forename: Elena
authors.initials: E
authors.lastname: Kocianová
authors.forename: Magda
authors.initials: M
authors.lastname: Tusek-Znidaric
authors.forename: Katarína
authors.initials: K
authors.lastname: Stefanidesová
authors.forename: Katarína
authors.initials: K
authors.lastname: Schwarzová
authors.forename: Marcela
authors.initials: M
authors.lastname: Kúdelová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Sekeyová
authors.forename: Eva
authors.initials: E
authors.lastname: Spitalská
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D003870:Dermacentor / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology*; D006801:Humans; D007739:L Cells; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins