title: Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions.
abstract: This study investigated conditions for optimal in vitro propagation of human skin-derived mesenchymal stem cells (S-MSC). Forty primary skin-derived precursor cell (SKP) cultures were established from both male and female donors (age 29-65 years) and eight of them were randomly selected for in-depth characterization. Effects of basic fibroblast growth factor (FGF-2), epidermal growth factor (EGF), leukemia inhibiting factor (LIF) and dibutyryl-cyclic adenosine monophosphate (db-cAMP) on S-MSC proliferation were investigated. Primary SKP cultures were >95% homogenous for CD90, CD73, and CD105 marker expression enabling to classify these cells as S-MSC. FGF-2 dose-dependent stimulation was observed in low serum medium only, whereas EGF neither stimulated S-MSC proliferation nor potentates the effect of FGF-2. Pronounced donor to donor differences among S-MSC cultures were observed in 3-day proliferation assay. This study demonstrates that homogenous S-MSC populations can be reproducibly isolated from individual donors of different age. Optimal cell culture conditions for in vitro propagation of S-MSC are B27 supplemented or low serum media with FGF-2 (4 ng/ml). EGF and LIF as well as db-cAMP are dispensable for S-MSC proliferation.
authors.affiliation: Faculty of Medicine, University of Latvia, 1a Sarlotes Street, Riga, 1001, Latvia, Una.Riekstina@lu.lv.
authors.forename: Una
authors.initials: U
authors.lastname: Riekstina
authors.forename: Ruta
authors.initials: R
authors.lastname: Muceniece
authors.forename: Inese
authors.initials: I
authors.lastname: Cakstina
authors.forename: Indrikis
authors.initials: I
authors.lastname: Muiznieks
authors.forename: Janis
authors.initials: J
authors.lastname: Ancans