title: Immune response against equine gammaherpesvirus in Icelandic horses.
abstract: Horses are hosts to two types of gammaherpesviruses, equine herpes virus (EHV) 2 and 5. While EHV-2 is ubiquitous in adult horses, EHV-5 has been less frequently described. Due to strong serological cross-reactivity, EHV-2 and -5 cannot be discriminated in broad spectrum antibody tests and are thus commonly referred to as gamma-EHV. Total IgG and IgG subclass response against gamma-EHV were determined in serum from 41 healthy Icelandic horses, thereof 20 adults, 10 foals aged 10 months, and 11 foals aged 1-4 months. Additionally, in 10 of the adult horses, interferon (IFN)-gamma and interleukin (IL)-4 expression were measured by real-time PCR in white blood cells upon in vitro stimulation with EHV-2. With the exception of one orphan foal, all tested individuals were seropositive for gamma-EHV. All but one adult had high titer of EHV-specific IgG4/7 (IgGb) in combination with much lower titer of IgG1 (IgGa) and IgG3/5 (IgG(T)), indicating a stabilized response. IgG titer and subclasses in the foals showed considerably more variation, possibly dependant on maternal antibodies and/or recent infection. In all the 10 horses tested for cytokine expression, IFN-gamma production exceeds production of IL-4. These results indicate that equine gammaherpesvirus infection is characterized by an induction of IgG1, IgG4/7 and IgG3/5 with prevailing IgG4/7 and cytokine profile dominated by IFN-gamma. To our knowledge, this is the first report on the cytokine and IgG subclass response against gamma-EHV in horses.
authors.affiliation: Institute for Experimental Pathology, University of Iceland, Keldur v/Vesturlandsveg, IS-112 Reykjavík, Iceland. vsvanss@hi.is
authors.forename: Vilhjálmur
authors.initials: V
authors.lastname: Svansson
authors.forename: Mieke
authors.initials: M
authors.lastname: Roelse
authors.forename: Gudbjörg
authors.initials: G
authors.lastname: Olafsdóttir
authors.forename: Lilja
authors.initials: L
authors.lastname: Thorsteinsdóttir
authors.forename: Einar G
authors.initials: EG
authors.lastname: Torfason
authors.forename: Sigurbjörg
authors.initials: S
authors.lastname: Torsteinsdóttir
mesh_terms: D017677:Age Distribution; D000818:Animals; D000914:Antibodies, Viral; D018142:Gammaherpesvirinae / Q000145:classification / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000276:immunology* / Q000821:virology; D006736:Horses; D007055:Iceland
chemical_list: D000914:Antibodies, Viral