Polymer photonic crystal dye lasers as Optofluidic Cell Sensors.
Dye doped hybrid polymer lasers are implemented as label free evanescent field biosensors for detection of cells. It is demonstrated that although the coverage is irregular and the cells extend over several lattice constants, the emission wavelength depends linearly on the fraction of the surface covered by the HeLa cells used as model system. Design parameters relating to photonic crystal sensing of large objects are identified and discussed. The lasers are chemically modified to bind cells and molecules with flexible UV activated linker molecules.
Christiansen
Mads Brøkner
MB
1Department of Micro and Nanotechnology, DTU Nanotech, Technical University of Denmark DK-2800 Kongens Lyngby, Denmark.
Lopacinska
Joanna Malgorzata
JM
Jakobsen
Mogens Havsteen
MH
Mortensen
Niels Asger
NA
Dufva
Martin
M
Kristensen
Anders
A
D015374:Biosensing Techniques / Q000295:instrumentation*; D002452:Cell Count / Q000295:instrumentation*; D017076:Computer-Aided Design; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011108:Polymers