Upregulated expression of miR-1/miR-206 in a rat model of myocardial infarction.
MicroRNAs (miRNAs) have been increasingly reported to have important roles in diverse biological and pathological processes. We investigated miR-1 and miR-206 expression and their potential roles in a rat model of myocardial infarction (MI). miR-1 and miR-206 expression were significantly increased, and insulin-like growth factor 1 (IGF-1) protein was markedly reduced without obvious change of its mRNA level after MI induction. Position 175-196 of rat IGF-1 3'-untranslated region was identified to be required for efficient downregulation by miR-1/miR-206. IGF-1 level was reduced without changing its transcript level in rat H9C2 myoblast cells modified with miR-1 (H9C2-miR-1). In the serum withdrawal and hypoxic condition, caspase-3 activity and mitochondrial potential were significantly increased in H9C2-miR-1 cells compared with the control group, respectively (p<0.05, p<0.01). Together, our results indicate that miR-1 and miR-206 are involved in apoptotic cell death in MI by post-transcriptional repression of IGF-1.
Shan
Zhi-Xin
ZX
Research Center of Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou, China.
Lin
Qiu-Xiong
QX
Fu
Yong-Heng
YH
Deng
Chun-Yu
CY
Zhou
Zhi-Ling
ZL
Zhu
Jie-Ning
JN
Liu
Xiao-Ying
XY
Zhang
You-Yi
YY
Li
Yangxin
Y
Lin
Shu-Guang
SG
Yu
Xi-Yong
XY
D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis*; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic; D015854:Up-Regulation
D035683:MicroRNAs; C522220:mirn206 microRNA, rat; D007334:Insulin-Like Growth Factor I