title: Isolation of two new prenylflavonols from Epimedium brevicornum and their effects on cytokine production in vitro.
abstract: In an attempt to search for bioactive natural products, two new prenylflavonols, 2''-hydroxy-3''-en-anhydroicaritin (1) and 2''-hydroxy-beta-anhydroicaritin (2), were isolated from the Chinese medicinal herb Epimedium brevicornum. Their structures were determined by 1D and 2D NMR spectroscopic analysis. The effects of compounds 1 and 2 on cytokine production in vitro were investigated. Compound 1 could significantly downregulate tumor necrosis factor-alpha (TNF-alpha) production and increase interleukin-10 (IL-10) production. These results suggested that compound 1 may have anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated mouse macrophages. In addition, the biogenetic relationships among compounds 1 and 2 are discussed.
authors.affiliation: College of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, P. R. China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Luo
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Ci
authors.forename: Rong
authors.initials: R
authors.lastname: Ren
authors.forename: Zhao-Yuan
authors.initials: ZY
authors.lastname: Wu
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.forename: Hai-Zhou
authors.initials: HZ
authors.lastname: Li
authors.forename: Rong-Tao
authors.initials: RT
authors.lastname: Li
authors.forename: Xu-Ming
authors.initials: XM
authors.lastname: Deng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D015536:Down-Regulation; D034181:Epimedium / Q000737:chemistry*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054876:Prenylation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C543986:2'-hydroxy-3'-en-anhydroicaritin; C543985:2'-hydroxy-beta-anhydroicaritin; D000893:Anti-Inflammatory Agents; D044948:Flavonols; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10