title: The actin-binding and bundling protein, EPLIN, is required for cytokinesis.
abstract: Cytokinesis involves two phases: (1) membrane ingression followed by (2) membrane abscission. The ingression phase generates a cleavage furrow and this requires co-operative function of the actin-myosin II contractile ring and septin filaments. We demonstrate that the actin-binding protein, EPLIN, locates to the cleavage furrow during cytokinesis and this is possibly via association with the contractile ring components, myosin II and the septin, Sept2. Depletion of EPLIN results in formation of multinucleated cells and this is associated with inefficient accumulation of active myosin II (MRLC(S19)) and Sept2 and their regulatory small GTPases, RhoA and Cdc42, respectively, to the cleavage furrow during the final stages of cytokinesis. We suggest that EPLIN may function during cytokinesis to maintain local accumulation of key cytokinesis proteins at the furrow.
authors.affiliation: Queensland Institute of Medical Research, Post Office Royal Brisbane Hospital, Brisbane, Queensland, Australia. mchircop@cmri.com.au
authors.forename: Megan
authors.initials: M
authors.lastname: Chircop
authors.forename: Vanessa
authors.initials: V
authors.lastname: Oakes
authors.forename: Mark E
authors.initials: ME
authors.lastname: Graham
authors.forename: Maggie P C
authors.initials: MP
authors.lastname: Ma
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Smith
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Robinson
authors.forename: Kum Kum
authors.initials: KK
authors.lastname: Khanna
mesh_terms: D000199:Actins / Q000378:metabolism; D045744:Cell Line, Tumor; D048749:Cytokinesis; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D024462:Myosin Type II / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; C403263:LIMA1 protein, human; D034741:RNA, Small Interfering; D010744:Phosphoric Monoester Hydrolases; D024462:Myosin Type II; D020764:cdc42 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein