Therapeutic activities of intravenous immunoglobulins in multiple sclerosis involve modulation of chemokine expression.
The objective of this study was to identify genes that are differentially expressed in peripheral T cells of patients with MS exacerbation receiving treatment with IVIG. Using microarray analysis, we identified 360 genes that were at least two-fold up- or down-regulated. The expression of four representative genes (PTGER4, CXCL5, IL11 and CASP2) was confirmed by quantitative PCR. Four of the differentially expressed genes encode chemokines (CXCL3, CXCL5, CCL13 and XCL2) that are involved in directing leukocyte migration. We suggest that the modulation of chemokine expression in peripheral T cells contributes to the beneficial activity of IVIG in patients with MS exacerbation.
Pigard
Nadine
N
BMT-Research GmbH, Vienna, Austria.
Elovaara
Irina
I
Kuusisto
Hanna
H
Paalavuo
Raija
R
Dastidar
Prasun
P
Zimmermann
Klaus
K
Schwarz
Hans-Peter
HP
Reipert
Birgit
B
D000328:Adult; D053143:Caspase 2 / Q000235:genetics; D054365:Chemokine CXCL5 / Q000235:genetics; D018925:Chemokines / Q000235:genetics*; D019743:Chemokines, CXC / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology; D017370:Interleukin-11 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000235:genetics; D058309:Receptors, Prostaglandin E, EP4 Subtype; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D054365:Chemokine CXCL5; D018925:Chemokines; D019743:Chemokines, CXC; C508611:IL11 protein, human; D016756:Immunoglobulins, Intravenous; D007155:Immunologic Factors; D017370:Interleukin-11; C545650:PTGER4 protein, human; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D058309:Receptors, Prostaglandin E, EP4 Subtype; C490360:CASP2 protein, human; D053143:Caspase 2; D003546:Cysteine Endopeptidases