Adding new dimensions to laser-scanning fluorescence microscopy.
We describe a novel method of optical imaging by exploiting simple ideas borrowed from pulsed optics. We show that the use of ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy dramatically brings together several advantages offered by two widely used present day microscopic techniques, confocal and multi-photon fluorescence microscopy. The method appears as a novel tool in the context of laser-scanning fluorescence microscopy by having a 'built-in' 3D spatial resolution.
De
A K
AK
Department of Chemistry, Indian Institute of Technology Kanpur, UP 208016, India.
Goswami
D
D
D000818:Animals; D002417:Cattle; D042783:Endothelial Cells / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007074:Immunoglobulin G / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D017785:Photons; D011651:Pulmonary Artery / Q000166:cytology / Q000648:ultrastructure
D005456:Fluorescent Dyes; D007074:Immunoglobulin G