In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method.
The antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from Goiania city patients, Brazil. In this study, the microtiter plates were incubated at 28 masculineC allowing a reading of the minimal inhibitory concentration (MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03 microg/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole, ketoconazole and terbinafine, respectively.
Araújo
Crystiane Rodrigues
CR
Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás, Goiânia, GO, Brasil. rosario@iptsp.ufg.br
Miranda
Karla Carvalho
KC
Fernandes
Orionalda de Fatima Lisboa
Ode F
Soares
Ailton José
AJ
Silva
Maria do Rosário Rodrigues
Mdo R
D000935:Antifungal Agents / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D008864:Microsporum / Q000145:classification / Q000187:drug effects*; D014249:Trichophyton / Q000145:classification / Q000187:drug effects*
D000935:Antifungal Agents; D017964:Itraconazole; D015725:Fluconazole; D007654:Ketoconazole