High-density peptide arrays.
Arrays promise to advance biology by allowing parallel screening for many different binding partners. Meanwhile, lithographic methods enable combinatorial synthesis of > 50,000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This review discusses recent developments in the field with an emphasis on methods that lead to very high-density peptide arrays.
Breitling
Frank
F
German Cancer Research Center, Im Neuenheimer Feld 580, Heidelberg, Germany. f.breitling@dkfz.de
Nesterov
Alexander
A
Stadler
Volker
V
Felgenhauer
Thomas
T
Bischoff
F Ralf
FR
D020650:Combinatorial Chemistry Techniques; D010455:Peptides / Q000138:chemical synthesis*; D040081:Protein Array Analysis; D011506:Proteins / Q000138:chemical synthesis
D010455:Peptides; D011506:Proteins