STOP proteins contribute to the maturation of the olfactory system.
Regulation of microtubule dynamics is crucial for axon growth and guidance as well as for the establishment of synaptic connections. STOPs (Stable Tubule Only Polypeptides) are microtubule-associated proteins that regulate microtubule stabilization but are also able to interact with actin or Golgi membranes. Here, we have investigated the involvement of STOPs during the development of the olfactory system. We first describe the spatio-temporal expression patterns of N- and E-STOP, the two neuronal-specific isoforms of STOP. E- and N-STOP are expressed in the axonal compartment of olfactory sensory neurons, but are differentially regulated during development. Interestingly, each neuronal isoform displays a specific gradient distribution within the olfactory nerve layer. Then, we have examined the development of the olfactory system in the absence of STOPs. Olfactory axons display a normal outgrowth and targeting in STOP-null mice, but maturation of the synapses in the glomerular neuropil is altered.
Richard
Marion
M
Laboratoire Neurosciences Sensorielles, Comportement, Cognition, CNRS-UMR 5020, Université de Lyon, Lyon 1, F-69366, France. marion.richard@yale.edu
Sacquet
Joëlle
J
Jany
Marion
M
Schweitzer
Annie
A
Jourdan
François
F
Andrieux
Annie
A
Pellier-Monnin
Véronique
V
D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009833:Olfactory Pathways / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques; D019147:Vomeronasal Organ / Q000166:cytology / Q000502:physiology
C113727:Map6 protein, rat; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms