title: The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria.
abstract: Prohibitin ring complexes in the mitochondrial inner membrane regulate cell proliferation as well as the dynamics and function of mitochondria. Although prohibitins are essential in higher eukaryotes, prohibitin-deficient yeast cells are viable and exhibit a reduced replicative life span. Here, we define the genetic interactome of prohibitins in yeast using synthetic genetic arrays, and identify 35 genetic interactors of prohibitins (GEP genes) required for cell survival in the absence of prohibitins. Proteins encoded by these genes include members of a conserved protein family, Ups1 and Gep1, which affect the processing of the dynamin-like GTPase Mgm1 and thereby modulate cristae morphogenesis. We show that Ups1 and Gep1 regulate the levels of cardiolipin and phosphatidylethanolamine in mitochondria in a lipid-specific but coordinated manner. Lipid profiling by mass spectrometry of GEP-deficient mitochondria reveals a critical role of cardiolipin and phosphatidylethanolamine for survival of prohibitin-deficient cells. We propose that prohibitins control inner membrane organization and integrity by acting as protein and lipid scaffolds.
authors.affiliation: Institute for Genetics, Centre for Molecular Medicine (CMMC), Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne 50674, Germany.
authors.forename: Christof
authors.initials: C
authors.lastname: Osman
authors.forename: Mathias
authors.initials: M
authors.lastname: Haag
authors.forename: Christoph
authors.initials: C
authors.lastname: Potting
authors.forename: Jonathan
authors.initials: J
authors.lastname: Rodenfels
authors.forename: Phat Vinh
authors.initials: PV
authors.lastname: Dip
authors.forename: Felix T
authors.initials: FT
authors.lastname: Wieland
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
authors.forename: Benedikt
authors.initials: B
authors.lastname: Westermann
authors.forename: Thomas
authors.initials: T
authors.lastname: Langer
mesh_terms: D000595:Amino Acid Sequence; D002308:Cardiolipins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000378:metabolism; D000091402:Prohibitins; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D002308:Cardiolipins; D010714:Phosphatidylethanolamines; D000091402:Prohibitins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C483858:phosphatidylethanolamine