Tissue engineering polymeric microcarriers with macroporous morphology and bone-bioactive surface.
PCL microspheres featuring a macroporous morphology and a bone-bioactive surface have been prepared. 'Camphene' was introduced to generate pores within the microsphere network. The pore size was variable from a few to tens to hundreds of microm depending on the Camphene/PCL ratio. Macropores (with sizes >50 microm) could be obtained with a Camphene/PCL ratio exceeding 6. The microsphere surface was further tailored with apatite mineral phase through solution-mediated precipitation, to endow the interface with bone bioactivity. Rat bone marrow stromal cells attached and spread actively on microspheres and populated well within their macropores. The developed microspheres may be potentially applicable as a cell delivery scaffold for bone tissue engineering.
Hong
Seok-Jung
SJ
Department of Biomaterials Science, School of Dentistry and Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Korea.
Yu
Hye-Sun
HS
Kim
Hae-Won
HW
D000818:Animals; D000081008:Bicyclic Monoterpenes; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001842:Bone and Bones / Q000737:chemistry*; D049109:Cell Proliferation; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D016062:Porosity; D051381:Rats; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D013499:Surface Properties; D013729:Terpenes / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D000081008:Bicyclic Monoterpenes; D013729:Terpenes; C019286:camphene