title: Relationship of neutrophil phagocytosis and oxidative burst with the subgingival microbiota of generalized aggressive periodontitis.
abstract: BACKGROUND
  Polymorphonuclear neutrophil (PMN) dysfunctions have been associated with severe forms of periodontitis. This study evaluated the correlation between PMN phagocytosis and oxidative burst with the subgingival microbiota of patients with generalized aggressive periodontitis (GAgP).
  
  
  METHODS
  Heparinized peripheral blood samples were obtained from 18 GAgP patients and 11 periodontally healthy (PH) subjects, and PMNs were isolated on a Ficoll-Hypaque gradient. For phagocytosis analysis, PMNs were incubated with fluorescein-labeled Staphylococcus aureus. The oxidative burst was evaluated by incubation of PMNs with dihydroethidium and activation by S. aureus. The assays were examined using flow cytometry. Subgingival biofilm samples were obtained from periodontal sites with and without periodontitis and 24 species were detected by checkerboard.
  
  
  RESULTS
  A significantly lower phagocytosis rate was observed for patients with GAgP compared with PH subjects over time (P < 0.05). No differences between groups were found for superoxide production. GAgP patients presented significantly higher prevalence and levels of Porphyromonas gingivalis, Tannerella forsythia, and Aggregatibacter actinomycetemcomitans serotype b than controls (P < 0.05). Significant negative correlations between T. forsythia and P. gingivalis and PMN functions were observed.
  
  
  CONCLUSIONS
  GAgP subjects presented diminished phagocytic activity of peripheral PMNs and high prevalence and levels of classical periodontal pathogens.
authors.affiliation: Department of Periodontology, Dental School of Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: R P M
authors.initials: RP
authors.lastname: Carvalho
authors.forename: J S
authors.initials: JS
authors.lastname: Mesquita
authors.forename: A
authors.initials: A
authors.lastname: Bonomo
authors.forename: P X
authors.initials: PX
authors.lastname: Elsas
authors.forename: A P V
authors.initials: AP
authors.lastname: Colombo
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology / Q000302:isolation & purification; D010520:Aggressive Periodontitis / Q000276:immunology* / Q000382:microbiology*; D001439:Bacteroides / Q000276:immunology / Q000302:isolation & purification; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010514:Periodontal Pocket / Q000382:microbiology*; D010587:Phagocytosis; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000302:isolation & purification; D016897:Respiratory Burst; D013291:Streptococcus / Q000276:immunology / Q000302:isolation & purification