title: Molecular diagnosis of disseminated adiaspiromycosis due to Emmonsia crescens.
abstract: Emmonsia crescens is a saprophytic fungus that is distributed worldwide, causing diseases mostly in rodents. It has also been described, though rarely, as an etiologic agent of pulmonary pathology in humans, potentially leading to death. A case of pulmonary adiaspiromycosis is reported in a 30-year-old immunocompetent man. The patient presented with a history of several weeks of weakness, cough, fever, and weight loss of 10 kg. Clinical and radiographic findings showed pulmonary lesions consistent with tuberculosis or histoplasmosis, but no pathogen was found with classical microbiological procedures. The diagnosis of adiaspiromycosis due to Emmonsia crescens was initially made using molecular biology techniques. Histological observations subsequently confirmed the presence of adiaspores in granulomas. To our knowledge, this is the first case of adiaspiromycosis diagnosed by PCR and sequencing. The patient was treated with itraconazole and was seen at 1 month with symptomatic improvement. Here we will discuss this rare fungal infection and its difficult treatment and diagnosis. As represented in this case, molecular biology is a powerful method to optimize diagnostic tests and therefore improve the care of the infected patient.
authors.affiliation: Service de Pneumologie, Hôpital d'Instruction des Armées Legouest, Metz Armées, France.
authors.forename: J-M
authors.initials: JM
authors.lastname: Dot
authors.forename: A
authors.initials: A
authors.lastname: Debourgogne
authors.forename: J
authors.initials: J
authors.lastname: Champigneulle
authors.forename: Y
authors.initials: Y
authors.lastname: Salles
authors.forename: M
authors.initials: M
authors.lastname: Brizion
authors.forename: J M
authors.initials: JM
authors.lastname: Puyhardy
authors.forename: J
authors.initials: J
authors.lastname: Collomb
authors.forename: F
authors.initials: F
authors.lastname: Plénat
authors.forename: M
authors.initials: M
authors.lastname: Machouart
mesh_terms: D000328:Adult; D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D002912:Chrysosporium / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005602:France; D006801:Humans; D017964:Itraconazole / Q000627:therapeutic use; D008168:Lung / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008969:Molecular Sequence Data; D013902:Radiography, Thoracic; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D017964:Itraconazole