Metabolism of hibifolin by human intestinal bacteria.
Hibifolin, the highest-content bioactive flavonoid of the flowers of Abelmoschus manihot, was incubated with human intestinal bacteria, and four metabolites (1-4) were obtained from the incubated solution by chromatographic methods. The structures of the four metabolites were elucidated as gossypetin 8-O-beta-D-4''-deoxy- Delta(4'')-glucuropyranoside (1), gossypetin (2), quercetin (3), and 8-methoxy-quercetin (4), respectively, on the basis of UV, NMR, and MS data. Metabolite 1 was obtained as a new compound with a specific beta-D-4''-deoxy-Delta(4'')-glucuropyranosyl moiety, which was formed through a unique and novel metabolic pathway that has not been reported previously.
Xu
Tong-Tong
TT
Department of Natural Medicines and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical , Sciences, Peking University Health Science Center, Beijing, P. R. China.
Yang
Xiu-Wei
XW
Wang
Bin
B
Xu
Wei
W
Zhao
Yu-Ying
YY
Zhang
Qing-Ying
QY
D028483:Abelmoschus / Q000737:chemistry; D001419:Bacteria / Q000378:metabolism*; D005419:Flavonoids / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009005:Monosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D011794:Quercetin / Q000302:isolation & purification / Q000378:metabolism*
C543378:8-O-beta-D-4'-deoxy-delta(4')-glucuropyranoside; D005419:Flavonoids; D009005:Monosaccharides; C087346:hibifolin; D011794:Quercetin; C059922:gossypetin