[Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis].
The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis. Cell viability was tested by trypan blue staining and apoptosis was detected by using flow cytometry. The expressions of CBL and CBL-b and the phosphorylation of SYK were detected by using immunoprecipitation and Western blot. The results showed that Bufalin inhibited HL-60 cell proliferation in time- and dose-dependent manners. IC(50) of suppressing cell viability at 24, 48 and 72 hours were about 26.3, 7.8 and 2.0 nmol/L respectively. The high dose of bufalin already induced apoptosis of HL-60 cells at 8 hours. SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin. It is concluded that SYK activation and CBL protein down-regulation may be involved in Bufalin-induced HL-60 cell apoptosis.
Qu
Xiu-Juan
XJ
Department of Medical Oncology, The First Clinical College, China Medical University, Shenyang 110001, China.
Zhao
Ming-Fang
MF
Liu
Yun-Peng
YP
Teng
Yue-E
YE
Qu
Jing-Lei
JL
Zhang
Ye
Y
Xu
Ling
L
Li
Ying-Chun
YC
Hou
Ke-Zuo
KZ
D017209:Apoptosis / Q000187:drug effects*; D002018:Bufanolides / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D050721:Proto-Oncogene Proteins c-cbl / Q000378:metabolism*; D015398:Signal Transduction; D000072377:Syk Kinase
D002018:Bufanolides; D047908:Intracellular Signaling Peptides and Proteins; D050721:Proto-Oncogene Proteins c-cbl; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; C067419:CBL protein, human; C022777:bufalin