title: CD4 microglial expression correlates with spontaneous clinical improvement in the acute Lewis rat EAE model.
abstract: CD4 is a molecule commonly expressed on the surface of T-helper lymphocytes with a recognized critical role in the antigen presentation process that has also been reported in monocytes and macrophages, although its role in these cells remains unknown. The objective of the present study was to analyze whether experimental conditions involving a potent acquired immune component, as occurs in experimental autoimmune encephalomyelitis (EAE), are able to induce CD4 expression in the population of microglia/macrophages. Myelin Basic Protein (MBP) immunized female Lewis rats, were examined at different phases during the course of EAE according to their clinical score. Spinal cords were analyzed by flow cytometry for CD11b, CD4 and CD45, by histochemistry for NDPase and by immunohistochemistry for ED2, Iba1, CD45 and CD4. Flow cytometry analysis showed that EAE induced CD4 expression in macrophages (CD11b+/CD45(high)) and microglia (in both CD11b+/CD45(intermediate) and CD11b+/CD45(low) phenotypes). Noticeably, microglial CD4 expression was found during the recovery phase and was maintained until 40 days post-induction. In agreement, immunolabelled sections revealed CD4 expression in microglial cells with ramified morphology during the recovery and post-recovery phases. In conclusion, our results indicate that, in this EAE model, perivascular cells, microglia and macrophages showed different dynamics during the course of the disease in close relation with symptomatology and that microglial cells expressed CD4 interestingly during the recovery phase, suggesting a role of microglial CD4 expression in the resolution of the immune response.
authors.affiliation: Unit of Histology, Department of Cell Biology, Physiology and Immunology, Institute of Neuroscience, Faculty of Medicine, Universitat Autònoma de Barcelona, Bellaterra, Spain. beatriz.almolda@uab.es
authors.forename: Beatriz
authors.initials: B
authors.lastname: Almolda
authors.forename: Manuela
authors.initials: M
authors.lastname: Costa
authors.forename: Maria
authors.initials: M
authors.lastname: Montoya
authors.forename: Berta
authors.initials: B
authors.lastname: González
authors.forename: Bernardo
authors.initials: B
authors.lastname: Castellano
mesh_terms: D000208:Acute Disease; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000378:metabolism; D018845:CD11 Antigens / Q000032:analysis / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism*; D002490:Central Nervous System / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000503:physiopathology; D005260:Female; D005434:Flow Cytometry; D005911:Gliosis / Q000276:immunology / Q000503:physiopathology; D007113:Immunity, Innate / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D017628:Microglia / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D020127:Recovery of Function / Q000276:immunology*; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology
chemical_list: D000954:Antigens, Surface; D018845:CD11 Antigens; D015704:CD4 Antigens; D017493:Leukocyte Common Antigens