Expansion of the sortase-mediated labeling method for site-specific N-terminal labeling of cell surface proteins on living cells.
The sortase-mediated labeling method, whose applications have been limited to C-terminal labeling of cell surface proteins, has been expanded for site-specific N-terminal labeling of cell surface proteins.
Yamamoto
Teruyasu
T
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Nagamune
Teruyuki
T
D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D015335:Molecular Probes; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases