title: Production, purification, and characterization of a fusion protein of carbonic anhydrase from Neisseria gonorrhoeae and cellulose binding domain from Clostridium thermocellum.
abstract: Carbon dioxide capture technologies have the potential to become an important climate change mitigation option through sequestration of gaseous CO2. A new concept for CO2 capture involves use of immobilized carbonic anhydrase (CA) that catalyzes the reversible hydration of CO2 to HCO3(-) and H+. Cost-efficient production of the enzyme and an inexpensive immobilization system are critical for development of economically feasible CA-based CO2 capture processes. An artificial, bifunctional enzyme containing CA from Neisseria gonorrhoeae and a cellulose binding domain (CBD) from Clostridium thermocellum was constructed with a His6 tag. The chimeric enzyme exhibited both CA activity and CBD binding affinity. This fusion enzyme is of particular interest due to its binding affinity for cellulose and retained CA activity, which could serve as the basis for improved technology to capture CO2 from flue gasses.
authors.affiliation: Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Liu
authors.forename: Patrick
authors.initials: P
authors.lastname: Bartlow
authors.forename: Robert M
authors.initials: RM
authors.lastname: Dilmore
authors.forename: Yee
authors.initials: Y
authors.lastname: Soong
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Pan
authors.forename: Richard
authors.initials: R
authors.lastname: Koepsel
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ataai
mesh_terms: D002256:Carbonic Anhydrases / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000235:genetics / Q000378:metabolism*; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D015202:Protein Engineering; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D002482:Cellulose; D002256:Carbonic Anhydrases