Purification and viral inactivation of hemoglobin from human placenta blood.
A new procedure was developed to obtain high-quality polymerized human hemoglobin by modifying purified hemoglobin with PLP and polymerized with GDA. Comparing polymerized hemoglobin products obtained from different methods, the product from the new procedure has similar physical, chemical, and biological properties in the molecular distribution, methemoglobin concentration, oxygen carrier capacity, P(50) and spectral analysis. Furthermore, the new procedure of modification after polymerization can save PLP greatly, and significantly reduce the cost. So the procedure of modification after polymerization is a better way in research and production.
Li
Fengjuan
F
Tianjin University, China.
Zhang
Honghui
H
Wang
Jinfeng
J
Yang
Chengmin
C
D001671:Biochemistry / Q000379:methods; D001774:Blood Chemical Analysis; D001802:Blood Substitutes; D002986:Clinical Trials as Topic; D003362:Cost-Benefit Analysis / Q000379:methods; D005260:Female; D005976:Glutaral / Q000737:chemistry; D006207:Half-Life; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008705:Methemalbumin / Q000737:chemistry* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010920:Placenta / Q000098:blood supply / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*; D011247:Pregnancy; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism
D001802:Blood Substitutes; D006454:Hemoglobins; D008705:Methemalbumin; D011108:Polymers; C030939:polyhemoglobin-pyridoxal-5-phosphate; D011732:Pyridoxal Phosphate; D010100:Oxygen; D005976:Glutaral