title: Community composition and density of methanogens in the foregut of the Tammar wallaby (Macropus eugenii).
abstract: The composition of the methanogenic archaeal community in the foregut contents of Tammar wallabies (Macropus eugenii) was studied using 16S rRNA and methyl coenzyme reductase subunit A (mcrA) gene clone libraries. Methanogens belonging to the Methanobacteriales and a well-supported cluster of uncultivated archaeon sequences previously observed in the ovine and bovine rumens were found. Methanogen densities ranged from 7.0 x 10(5) and 3.9 x 10(6) cells per gram of wet weight.
authors.affiliation: CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Brisbane, Queensland 4067, Australia. Paul.Evans@csiro.au
authors.forename: Paul N
authors.initials: PN
authors.lastname: Evans
authors.forename: Lyn A
authors.initials: LA
authors.lastname: Hinds
authors.forename: Lindsay I
authors.initials: LI
authors.lastname: Sly
authors.forename: Christopher S
authors.initials: CS
authors.lastname: McSweeney
authors.forename: Mark
authors.initials: M
authors.lastname: Morrison
authors.forename: André-Denis G
authors.initials: AD
authors.lastname: Wright
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics; D044822:Biodiversity; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology*; D020459:Genes, rRNA; D007614:Macropodidae / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D008697:Methane