title: [Cytogenetic analysis of 362 cases of chronic myeloid leukemia].
abstract: In order to evaluate the cytogenetic features and clinical significance of chronic myeloid leukemia (CML), chromosome preparation of bone marrow cells was made by using 24-hour culture, and R-banding technique was employed for karyotyping in 362 patients with CML. The patients were divided into two groups of chronic phase (CP) and blast crisis (BC). The results showed that the incidence of additional chromosome, variant translocation and Philadelphia (Ph) negative, bcr/abl positive CML with abnormal chromosomes in CP group were 70 cases (26.1%), 19 cases (7.1%), 4 cases (1.5%), and were 50 cases (53.2%), 8 cases (8.5%), 4 cases (4.3%) in BC group. Among the 362 cases, 324 cases (89.5%) were Ph positive. Classic translocation was found in 297 cases (91.7%) and variant translocation in 27 cases (8.3%), including 13 cases of simple variant, 13 cases of complex variant and 1 case of marked Ph. Special karyotypes were found in 120 out of 362 cases. Analysis of these karyotypes demonstrated that the most common numerical abnormalities were +Ph (21.7%), +8 (10.0%), +21 (10.0%), +19 (7.5%) and structure abnormalities were i(17q) (13.3%). In conclusion, compared to chronic phase, the incidence of additional chromosome, variant translocation and so on are much higher at in blast crisis. It is feasible to evaluate the progress of the disease by karyotype analysis.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People Hospital, Nanjing 210029, China.
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Qiu
authors.forename: Kou-Rong
authors.initials: KR
authors.lastname: Miao
authors.forename: Si-Xuan
authors.initials: SX
authors.lastname: Qian
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Hong
authors.forename: Chun
authors.initials: C
authors.lastname: Qiao
authors.forename: Jian-Fu
authors.initials: JF
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
authors.forename: Han-Xin
authors.initials: HX
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Qiu
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Su-Jiang
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D005260:Female; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D055815:Young Adult