Phenomenological partial-specific volumes for G-quadruplex DNAs.
Accurate partial-specific volume ([Formula: see text]) values are required for sedimentation velocity and sedimentation equilibrium analyses. For nucleic acids, the estimation of these values is complicated by the fact that [Formula: see text] depends on base composition, secondary structure, solvation and the concentrations and identities of ions in the surrounding buffer. Here we describe sedimentation equilibrium measurements of the apparent isopotential partial-specific volume /' for two G-quadruplex DNAs and a single-stranded DNA of similar molecular weight and base composition. The G-quadruplex DNAs are a 22 nucleotide fragment of the human telomere consensus sequence and a 27 nucleotide fragment from the human c-myc promoter. The single-stranded DNA is 26 nucleotides long and is designed to have low propensity to form secondary structures. Parallel measurements were made in buffers containing NaCl and in buffers containing KCl, spanning the range 0.09 M <or= [salt] <or= 2.3 M. Limiting values of /', extrapolated to [salt] = 0 M, were: 22-mer (NaCl-form), 0.525 +/- 0.004 mL/g; 22-mer (KCl-form), 0.531 +/- 0.006 mL/g; 27-mer (NaCl-form), 0.548 +/- 0.005 mL/g; 27-mer (KCl-form), 0.557 +/- 0.006 mL/g; 26-mer (NaCl-form), 0.555 +/- 0.004 mL/g; 26-mer (KCl-form), 0.564 +/- 0.006 mL/g. Small changes /' with [salt] suggest that large changes in counterion association or hydration are unlikely to take place over these concentration ranges.
Hellman
Lance M
LM
Department of Molecular and Cellular Biochemistry, Center for Structural Biology, University of Kentucky, 741 South Limestone, Lexington, KY 40536-0509, USA.
Rodgers
David W
DW
Fried
Michael Gregory
MG
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D054856:G-Quadruplexes; D006801:Humans; D008958:Models, Molecular; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011189:Potassium Chloride / Q000737:chemistry; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry / Q000235:genetics; D012965:Sodium Chloride / Q000737:chemistry; D016615:Telomere / Q000235:genetics
D004277:DNA, Single-Stranded; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012965:Sodium Chloride; D011189:Potassium Chloride; D004247:DNA