Molecular identification of Mycobacterium chimaera as a cause of infection in a patient with chronic obstructive pulmonary disease.
This report describes a case of Mycobacterium chimaera infection in a patient with a history of chronic obstructive pulmonary disease where the organism was identified by using molecular methods. M. chimaera was identified from fresh lung tissue and from an instrument-negative mycobacterial growth indicator tube broth culture. The utility of using sequence analysis of the internal transcribed spacer region for the rapid identification of a slow-growing nontuberculous Mycobacterium spp. where conventional culture methods were not successful was shown.
Bills
Nathan D
ND
i(3)Bio, LLC, Omaha, NE 68022, USA.
Hinrichs
Steven H
SH
Aden
Tricia A
TA
Wickert
Robert S
RS
Iwen
Peter C
PC
D000368:Aged; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004864:Equipment and Supplies / Q000382:microbiology; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer