title: Combination of Zizyphus jujuba and green tea extracts exerts excellent cytotoxic activity in HepG2 cells via reducing the expression of APRIL.
abstract: Hepatocellular carcinoma is a type of tumor highly resistant to available chemotherapeutic agents. The treatment of hepatocellular carcinoma remains a challenge that needs new approaches in the future. In a previous study, we demonstrated that the chloroform fraction (CHCl(3)-F) from Z. jujuba has anticancer activity in human liver cancer cells (HepG2), and that combining CHCl(3)-F with green tea extracts (GTE) results in enhanced effects of anticancer activity in the cells. To further understand the mechanism of the anticancer activity of combining CHCl(3)-F and GTE in HepG2 cells, we investigated whether the addition of a mixture of CHCl(3)-F and GTE would affect the expression of APRIL (a proliferation-inducing ligand), which was expressed in HepG2 cells from 4 hours of incubation in vitro. We have shown that CHCl(3)-F and GTE enhanced anti-cancer activity by reducing the expression of APRIL. We speculate that the CHCl(3)-F and GTE mixture might provide a lead to a new drug design to treat hepatocellular carcinoma in the future.
authors.affiliation: Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Xuedan
authors.initials: X
authors.lastname: Huang
authors.forename: Akiko
authors.initials: A
authors.lastname: Kojima-Yuasa
authors.forename: Shenghui
authors.initials: S
authors.lastname: Xu
authors.forename: David Opare
authors.initials: DO
authors.lastname: Kennedy
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Hasuma
authors.forename: Isao
authors.initials: I
authors.lastname: Matsui-Yuasa
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology / Q000627:therapeutic use*; D028241:Camellia sinensis; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002470:Cell Survival; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D013662:Tea; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000378:metabolism*; D031957:Ziziphus
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D013662:Tea; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13