A novel tyrosinase biosensor based on biofunctional ZnO nanorod microarrays on the nanocrystalline diamond electrode for detection of phenolic compounds.
A novel tyrosinase biosensor based on biofuncational ZnO nanorod microarrays on the boron-doped nanocrystalline diamond (BDND) substrates was developed. The ZnO nanorod microarrays were firstly deposited on BDND thin film surfaces via a low-temperature solution method, and then ZnO nanorods were functionalized with the mixture of 3-aminopropyltriethoxysilane (APTES) and tetraethoxysilane (TEOS) by a co-condensation approach, then tyrosinase was immobilized to amino-modification ZnO nanorod surfaces by the covalent binding. As-prepared tyrosinase biosensors were used for the detection of phenolic compounds. The tyrosinase-modified BDND electrode gave a linear response range of 1-175, 1-150 and 1-150 microM and sensitivity of 576.2, 339.3 and 287.1 microA mmol(-1) cm(-2) for p-cresol, 4-chlorophenol and phenol, respectively. The low detection limit was estimated to be 0.1, 0.25 and 0.2 microM (s(b)/m=3), respectively. Therefore, the biofunctional ZnO nanorod arrays have potential applications as platforms to immobilize other enzymes and bioactive molecules in biosensors.
Zhao
Jianwen
J
College of Chemistry and Chemical Engineering, Jishou University, Jishou, Hunan Province, 416000, PR China. zhjw76@hotmail.com
Wu
Daohong
D
Zhi
Jinfang
J
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D001895:Boron / Q000737:chemistry; D018130:Diamond; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D013057:Spectrum Analysis
D004800:Enzymes, Immobilized; D010636:Phenols; D018130:Diamond; D014442:Monophenol Monooxygenase; D001895:Boron