title: Specific Removal of Monocytes from Peripheral Blood of Septic Patients by Polymyxin B-immobilized Filter Column.
abstract: Lipopolysaccharide (LPS) is one of the major causes of septic shock. The polymyxin B-immobilized filter column (PMX) was developed for the adsorption of endotoxin by direct hemoperfusion and has been used for the treatment of LPS-induced septic shock. In this study, we demonstrated that PMX also specifically bound monocytes from the peripheral blood leukocytes of septic patients by mean of an analysis of bound cells using immunocytochemical and electron microscopic techniques. The specific removal of monocytes from septic patients may produce beneficial effects by reducing the interaction between monocytes and functionally associated cells including vascular endothelial cells.
authors.affiliation: Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. mbori@md.okayama-u.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishibori
authors.forename: Hideo K
authors.initials: HK
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katayama
authors.forename: Shuji
authors.initials: S
authors.lastname: Mori
authors.forename: Shinya
authors.initials: S
authors.lastname: Saito
authors.forename: Hiromi
authors.initials: H
authors.lastname: Iwagaki
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Morita
authors.forename: Aiji
authors.initials: A
authors.lastname: Ohtsuka
mesh_terms: D000327:Adsorption; D000368:Aged; D039481:CD11b Antigen / Q000097:blood; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005374:Filtration; D006801:Humans; D019041:L-Selectin / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D011112:Polymyxin B / Q000737:chemistry*; D018805:Sepsis / Q000097:blood / Q000628:therapy*
chemical_list: D039481:CD11b Antigen; C509295:ITGAM protein, human; D019041:L-Selectin; D011112:Polymyxin B