Nevirapine resistance in human immunodeficiency virus type 1-positive infants determined using dried blood spots stored for up to six years at room temperature.
Dried blood spots that had been stored ambiently for 3 to 6 years lost approximately 1 log(10) of human immunodeficiency virus type 1 (HIV-1) RNA, but the majority could still be genotyped for resistance. Nevirapine resistance was found in 7/16 (43.5%) HIV-1-positive HIVNET 024 infants at 4 to 6 weeks, but no resistance was found at other time points.
Nelson
Julie A E
JA
Department of Microbiology and Immunology, UNC Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. jnelson@med.unc.edu
Loftis
Amy M
AM
Kamwendo
Deborah
D
Fawzi
Wafaie W
WW
Taha
Taha E
TE
Goldenberg
Robert L
RL
Fiscus
Susan A
SA
D019380:Anti-HIV Agents / Q000494:pharmacology*; D001769:Blood / Q000821:virology*; D003890:Desiccation; D024882:Drug Resistance, Viral; D005838:Genotype; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019829:Nevirapine / Q000494:pharmacology*; D012367:RNA, Viral / Q000235:genetics*; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature
D019380:Anti-HIV Agents; D012367:RNA, Viral; D019829:Nevirapine