Development of a non-aqueous electrophoresis method for the simultaneous determination of tricyclic antidepressants in human serum.
In the present work we report a non-aqueous electrophoresis method (NACE) for the separation and determination of eight tricyclic antidepressants (TCAs) in human serum. Separation is carried out in 1 M acetic acid, 50 mM ammonium acetate and 10 mM SDS in ACN. Standards and samples were prepared in 0.8 M acetic acid in ACN and introduced by electrokinetic injection. The interactions between TCAs and acetate ions were studied and deduced from comparison of both the actual experimental and theoretical mobility. The establishment of calibration curves in the presence or absence of serum matrix, between 20 and 200 ng/mL, together with the determination of most important analytical parameters and a study of robustness were performed. Validation of the CE method was carried out with serum samples spiked with the analytes. The NACE determination method offers better resolution and higher sensitivity than the determination of TCAs in aqueous media.
Acedo-Valenzuela
María-Isabel
MI
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Extremadura, Spain. miacedo@unex.es
Galeano-Díaz
Teresa
T
Rodríguez
Antonio Silva
AS
Martínez
Rita Carabias
RC
Alvarez
Javier Domínguez
JD
D019342:Acetic Acid / Q000737:chemistry; D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002138:Calibration; D003198:Computer Simulation; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D000929:Antidepressive Agents, Tricyclic; D012967:Sodium Dodecyl Sulfate; D019342:Acetic Acid