Gel purification of radiolabeled nucleic acids via phosphorimaging: Dip-N-Dot.
RNA and DNA oligonucleotides radiolabeled with (32)P or (33)P often require gel electrophoresis to remove undesired side and/or degradation products. Common ways to visualize these molecules after electrophoresis are by ultraviolet (UV) shadowing, which necessarily reduces the specific activity of the oligonucleotide, and by autoradiography using film, which is cumbersome and increases the cost of generating the radiolabeled molecule. A more cost-effective method is to physically inject the gel with a "Dip-N-Dot" solution of dye and radionuclide after electrophoresis but prior to phosphorimaging. The gel can be overlaid on its computer-generated image, allowing the labeled molecules to be visualized quickly.
Burton
Aaron S
AS
Department of Chemistry, Portland State University, Portland, OR 97207, USA.
Madix
Rowan A
RA
Vaidya
Nilesh
N
Riley
Craig A
CA
Hayden
Eric J
EJ
Chepetan
Andre
A
Arenas
Carolina Díaz
CD
Larson
Brian C
BC
Lehman
Niles
N
D001345:Autoradiography / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry*; D010761:Phosphorus Radioisotopes / Q000737:chemistry*
D009696:Nucleic Acids; D010761:Phosphorus Radioisotopes