Identification and quantitation of human amniotic fluid components using capillary zone electrophoresis.
A capillary electrophoresis method was used to measure albumin, immunoglobulin G (IgG), transferrin, and uric acid in 230 amniotic fluid (AF) samples collected at 15.15+/-0.06 weeks gestation. Species were quantified by external calibration using thiamine as internal standard. All major components were detected within 10 min. Migration time reproducibility was 3.0% relative standard deviation (RSD) and normalized peak areas were 12% RSD or better at 190 nm from 81 measurements of a pooled AF sample. The separation profile was not affected by 10h of storage at room temperature or by 10 freeze-thaw cycles, suggesting that frozen AF samples are suitable for protein biomarker studies.
Gao
Tao
T
School of Dietetics and Human Nutrition, McGill University (Macdonald Campus), Montreal, Quebec H9X-3V9, Canada.
Zablith
Nadine
N
Burns
David H
DH
Koski
Kristine G
KG
Skinner
Cameron D
CD
D000653:Amniotic Fluid / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005615:Freezing; D005865:Gestational Age; D006801:Humans; D011247:Pregnancy; D013831:Thiamine / Q000737:chemistry; D013997:Time Factors
D013831:Thiamine