Functional characterization of kanB by complementing in engineered Streptomyces fradiae Deltaneo6::tsr.
A putative aminotransferase gene, kanB, lies in the biosynthetic gene cluster of Streptomyces kanamyceticus ATCC 12853 and has 66% identity with neo6 in neomycin biosynthesis. Streptomyces fradiae Deltaneo6::tsr was generated by disrupting neo6 in the neomycin producer Streptomyces fradiae. Neomycin production was completely abolished in the disruptant mutant but was restored through self-complementation of neo6. S. fradiae HN4 was generated through complementation with kanB in Streptomyces fradiae neo6::tsr. Based on metabolite analysis by ESI/MS and LC/MS, neomycin production was restored in Streptomyces fradiae HN4. Thus, like neo6, kanB also functions as a 2-deoxy-scyllo-inosose aminotransferase that has dual functions in the formation of 2-deoxy-scyllo-inosose (DOS).
Jnawali
Hum Nath
HN
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University, 100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Korea.
Subba
Bimala
B
Liou
Kwangkyoung
K
Sohng
Jae Kyung
JK
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D017353:Gene Deletion; D005816:Genetic Complementation Test; D007294:Inositol / Q000031:analogs & derivatives / Q000378:metabolism; D009355:Neomycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D000637:Transaminases / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D007294:Inositol; C075548:2-deoxyinosose; D000637:Transaminases; D009355:Neomycin