Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli.
Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 wa scloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This allergen was purified by immobilized Ni2+-affinity chromatography. The purified Pen b 26 was characterized by immunological, biochemical and biophysical methods. C-His6 tagged Pen b 26 produced several fold greater yield than N-His6 tagged Pen b 26. The affinity of C-His6 tagged Pen b 26 for the specific antibody was also 2.75 times higher than N-His6 tagged Pen b 26
Sevinc
M Serdal
MS
Healthy Environments and Consumer Safety Branch, Health Canada, 251 F.G. Banting Driveway, Rm C229, Tunney's Pasture, Postal Locator 2202D2, Ottawa, Ont. K1A0K9, Canada. serdal_sevinc@hc-sc.gc.ca
Kumar
Veena
V
Abebe
Makonnen
M
Mohottalage
Susantha
S
Kumarathasan
Premkumari
P
Vincent
Renaud
R
Vijay
Hari M
HM
D000485:Allergens / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001249:Asthma / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D015870:Gene Expression; D010407:Penicillium / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D000485:Allergens; D005656:Fungal Proteins; D011994:Recombinant Proteins