Role of OsHAL3 protein, a putative 4'-phosphopantothenoylcysteine decarboxylase in rice.
In this study, we cloned the OsHAL3 gene from rice Oryza sativa. Alignment analysis revealed that OsHAL3 has a high sequence identity to Dfp protein in Escherichia coli and AtHAL3a protein in Arabidopsis thaliana, which have 4'-phosphopantothenoylcysteine decarboxylase (PPC-DC) activity. OsHAL3 can complement mutation in the E. coli dfp gene encoding PPC-DC, so that the mutant strains with OsHAL3 can grow on rich media at 42 degrees C and on VB minimal media at 30 degrees C. Complementation tests with point mutations of OsHAL3 suggested that the conserved Cys176 residue of OsHAL3 is a key active-site residue. The mutant OsHAL3 G180A has a partly reduced activity. Related mRNA-level analysis showed that the OsHAL3 gene is induced by calcium pantothenate in rice.
Zhang
Ning
N
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100094, China.
Wang
Xuechen
X
Chen
Jia
J
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010205:Pantothenic Acid / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D013997:Time Factors
D010940:Plant Proteins; D010205:Pantothenic Acid; D002262:Carboxy-Lyases; C022373:Phosphopantothenoyl-cysteine decarboxylase