title: Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms.
abstract: Talins are large adaptor proteins that link the integrin family of adhesion molecules to F-actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin-mediated cell adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian talin 2. This reveals the existence of a previously unrecognized promoter associated with a CpG island, and separated from the first coding exon by numerous alternatively spliced noncoding exons spanning > 200 kb. Analysis of a mouse gene trap line shows that this promoter accounts for most of the talin 2 expression in adult tissues. We also demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N-terminal half of the protein, and provide evidence for the developmentally regulated expression of the short testis-specific talin 2 isoform in elongating spermatids. Finally, we identify four tissue-specific alternative splicing events within the coding region of talin 2.
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester, UK.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Debrand
authors.forename: Yasmine
authors.initials: Y
authors.lastname: El Jai
authors.forename: Lorraine
authors.initials: L
authors.lastname: Spence
authors.forename: Neil
authors.initials: N
authors.lastname: Bate
authors.forename: Uta
authors.initials: U
authors.lastname: Praekelt
authors.forename: Catrin A
authors.initials: CA
authors.lastname: Pritchard
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Monkley
authors.forename: David R
authors.initials: DR
authors.lastname: Critchley
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D004247:DNA; D005091:Exons; D055785:Gene Knockdown Techniques; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013087:Spermatids / Q000378:metabolism; D016608:Talin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; C518833:TLN2 protein, mouse; D016608:Talin; D004247:DNA