Infectivity and propagation of attenuated infectious bursal disease virus in the chicken B-lymphocyte cell line DT40.
In this paper, the infectivity and propagation of two attenuated infectious bursal disease virus (IBDV) strains in DT40 cells were investigated. The results showed that both of the tested strains, TAD and HN(3), directly infect and proliferate in DT40 cells, requiring no adaptive passages. Unexpectedly, IBDV can be rapidly propagated and continuously harvested at high titers for a long time, accompanied by the rapid growth of host cells and showing no increase in pathogenicity. Our results provide further support to suggest that DT40 cells can be used as an ideal model for studying IBDV pathogenesis. Additionally, the DT40 cell line could also serve as a potential system for commercial IBDV vaccine preparation.
Luo
Jun
J
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, People's Republic of China. luojun593@yahoo.com.cn
Zhang
Gai-Ping
GP
Fan
Jian-Ming
JM
Teng
Man
M
You
Lei-Ming
LM
Zhou
Ling
L
Deng
Rui-Guang
RG
Wang
Xuan-Nian
XN
Yang
Yan-Yan
YY
Wang
Li
L
Xing
Guang-Xu
GX
Cheng
Na
N
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D002645:Chickens; D007243:Infectious bursal disease virus / Q000254:growth & development*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000235:genetics; D014776:Virus Cultivation / Q000379:methods
D015678:Viral Structural Proteins