title: Drosophila DHR38 nuclear receptor is required for adult cuticle integrity at eclosion.
abstract: DHR38 is the only Drosophila member of the NR4A subclass of vertebrate nuclear receptors, which have been implicated in multiple biological pathways, including neuronal function, apoptosis, and metabolism. Although an earlier study identified three point mutations in DHR38, none of these were shown to be a null allele for the locus, leaving it unclear whether a complete loss of DHR38 function might uncover novel roles for the receptor. Here we show that a specific DHR38 null allele, DHR38(Y214), leads to fully penetrant pharate adult lethality, similar to the most severe phenotype associated with the EMS-induced mutations. DHR38(Y214) mutants display minor effects on ecdysone-regulated transcription at the onset of metamorphosis. In contrast, cuticle gene expression is significantly reduced in DHR38(Y214) mutant pupae. These studies define the essential functions of DHR38 and provide a genetic context for further characterization of its roles during development.
authors.affiliation: Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112-5330, USA.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kozlova
authors.forename: Geanette
authors.initials: G
authors.lastname: Lam
authors.forename: Carl S
authors.initials: CS
authors.lastname: Thummel
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D004440:Ecdysone / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D034582:Integumentary System / Q000254:growth & development*; D009154:Mutation / Q000235:genetics; D011679:Pupa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C095176:Hr38 protein, Drosophila; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D004440:Ecdysone