A novel strategy for obtaining kanamycin resistance in Arabidopsis thaliana by silencing an endogenous gene encoding a putative chloroplast transporter.
The use of bacterial antibiotic resistance markers in transgenic plants raises concerns about horizontal gene transfer to soil bacteria. We report here that kanamycin resistance in Arabidopsis thaliana can be achieved by silencing an endogenous gene encoding a putative chloroplast transporter, which presumably imports kanamycin into chloroplasts to interfere with ribosomal RNA. Homologs of the transporter exist in other plant species, suggesting this strategy may be generally useful for selecting transformed plant cells.
Aufsatz
Werner
W
Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria.
Nehlin
Lilian
L
Voronin
Viktor
V
Schmidt
Agnes
A
Matzke
Antonius J M
AJ
Matzke
Marjori
M
D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D060365:Chloroplast Proteins; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D020868:Gene Silencing / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D007612:Kanamycin / Q000008:administration & dosage*; D026901:Membrane Transport Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000502:physiology*
D000900:Anti-Bacterial Agents; D060365:Chloroplast Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D007612:Kanamycin