title: Cryopreservation of Spanish ibex (Capra pyrenaica) sperm obtained by electroejaculation outside the rutting season.
abstract: The effectiveness of electroejaculation for obtaining Spanish ibex sperm samples for freeze preserving outside the rutting season was evaluated-the aim being to optimise biological resources for the establishment of germplasm banks. The effect of different egg yolk concentrations (6% or 12%, v/v) in diluents of different buffer composition (Tris-citric acid buffer or Tes-Tris buffer) on frozen-thawed samples of the above also investigated. Experiments were undertaken with six ibex males in February-May, and involved four different semen samples from each animal with four combination of extender, respectively: Tes-Tris-glucose (TTG)-6% egg yolk, TTG-12% egg yolk, Tris-citric acid-glucose (TCG)-12% egg yolk, TCG-6% egg yolk. The results show that electroejaculation is a useful way of obtaining sperm samples from Spanish ibex outside the rutting season (i.e., at a time coinciding with plasma testosterone levels of <0.4ng/ml). According to the results of the eosin-nigrosin staining and the hypo-osmotic swelling test, the freezing-thawing process significantly reduced the viability and membrane integrity of the spermatozoa extended with TTG-6% egg yolk, TTG-12% egg yolk, and TCG-12% egg yolk, but did not affect these variables in spermatozoa extended with TCG-6% egg yolk. Therefore, the use of Tris-citric acid-based extenders containing low concentrations of egg yolk is recommended for cryopreserving Spanish ibex spermatozoa obtained by electroejaculation outside the rutting season.
authors.affiliation: Departamento de Reproducción Animal, Avda. Puerta de Hierro Km 5.9, INIA, 28040 Madrid, Spain. moreno@inia.es
authors.forename: J
authors.initials: J
authors.lastname: Santiago-Moreno
authors.forename: M A
authors.initials: MA
authors.lastname: Coloma
authors.forename: J
authors.initials: J
authors.lastname: Dorado
authors.forename: A
authors.initials: A
authors.lastname: Pulido-Pastor
authors.forename: F
authors.initials: F
authors.lastname: Gómez-Guillamon
authors.forename: R
authors.initials: R
authors.lastname: Salas-Vega
authors.forename: A
authors.initials: A
authors.lastname: Gómez-Brunet
authors.forename: A
authors.initials: A
authors.lastname: López-Sebastián
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004542:Ejaculation / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D006041:Goats / Q000502:physiology; D008297:Male; D012621:Seasons; D012662:Semen Preservation / Q000379:methods*; D012726:Sexual Behavior, Animal / Q000502:physiology; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000187:drug effects; D013739:Testosterone / Q000097:blood; D014325:Tromethamine / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; C077847:TES-Tris yolk buffer; D014325:Tromethamine; D013739:Testosterone; D005947:Glucose