title: Life with and without AtTIP1;1, an Arabidopsis aquaporin preferentially localized in the apposing tonoplasts of adjacent vacuoles.
abstract: The Arabidopsis thaliana Tonoplast Intrinsic Protein 1;1 (AtTIP1;1) is a member of the tonoplast aquaporin family. The tissue-specific expression pattern and intracellular localization of AtTIP1;1 were characterized using GUS and GFP fusion genes. Results indicate that AtTIP1;1 is expressed in almost all cell types with the notable exception of meristematic cells. The highest level of AtTIP1;1 expression was detected in vessel-flanking cells in vascular bundles. AtTIP1;1-GFP fusion protein labelled the tonoplast of the central vacuole and other smaller peripheral vacuoles. The fusion protein was not found evenly distributed along the tonoplast continuum but concentrated in contact zones of tonoplasts from adjacent vacuoles and in invaginations of the central vacuole. Such invaginations may result from partially engulfed small vacuoles. A knockout mutant was isolated and characterized to gain insight into AtTIP1;1 function. No phenotypic alteration was found under optimal growth conditions indicating that AtTIP1;1 function is not essential to the plant and that some members of the TIP family may act redundantly to facilitate water flow across the tonoplast. However, a conditional root phenotype was observed when mutant plants were grown on a glycerol-containing medium.
authors.affiliation: UMR INRA 1088/CNRS 5184/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065, Dijon, France. azeez.beebo@u-bourgogne.fr
authors.forename: Azeez
authors.initials: A
authors.lastname: Beebo
authors.forename: Dominique
authors.initials: D
authors.lastname: Thomas
authors.forename: Christophe
authors.initials: C
authors.lastname: Der
authors.forename: Lisa
authors.initials: L
authors.lastname: Sanchez
authors.forename: Nathalie
authors.initials: N
authors.lastname: Leborgne-Castel
authors.forename: Francis
authors.initials: F
authors.lastname: Marty
authors.forename: Benoît
authors.initials: B
authors.lastname: Schoefs
authors.forename: Karim
authors.initials: K
authors.lastname: Bouhidel
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D016254:Mutagenesis, Insertional; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism*
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C506684:TIP1 protein, Arabidopsis