title: Repeated elements coordinate the spatial organization of the yeast genome.
abstract: The spatial organization of the chromosomes is crucial for gene expression and development. Inter- and intrachromosomal interactions form a crucial part of this epigenomic regulatory system. Here we use circular chromosome conformation capture-on-chip (4C) to identify interactions between repetitive and non-repetitive loci within the yeast genome. The interacting regions occur in non-randomly distributed clusters. Furthermore, the SIR2 histone deacetylase has opposing roles in the organization of the inter- or intrachromosomal interactions. These data establish a dynamic domain model for yeast genome organization. Moreover, they point to the repeated elements playing a central role in the dynamic organization of genome architecture.
authors.affiliation: Institute of Molecular Biosciences, Massey University, Albany, New Zealand. j.m.osullivan@massey.ac.nz
authors.forename: J M
authors.initials: JM
authors.lastname: O'Sullivan
authors.forename: D M
authors.initials: DM
authors.lastname: Sontam
authors.forename: R
authors.initials: R
authors.lastname: Grierson
authors.forename: B
authors.initials: B
authors.lastname: Jones
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D045584:Chromosome Positioning; D004251:DNA Transposable Elements / Q000235:genetics / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal / Q000235:genetics* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements