title: Dietary n-3 and n-6 PUFA enhance DHA incorporation in retinal phospholipids without affecting PGE(1) and PGE (2) levels.
abstract: The purpose of this study was to determine whether dietary n-3 and n-6 PUFA may affect retinal PUFA composition and PGE(1) and PGE(2) production. Male Wistar rats were fed for 3 months with diets containing: (1) 10% eicosapentaenoic acid (EPA) and 7% docosahexaenoic acid (DHA), or (2) 10% gamma-linolenic acid (GLA), or (3) 10% EPA, 7% DHA and 10% GLA, or (4) a balanced diet deprived of EPA, DHA, and GLA. The fatty acid composition of retinal phospholipids was determined by gas chromatography. Prostaglandin production was measured by enzyme immunoassay. When compared to rats fed the control diet, the retinal levels of DHA were increased in rats fed both diets enriched with n-3 PUFA (EPA + DHA and EPA + DHA + GLA diets) and decreased in those supplemented with n-6 PUFA only (GLA diet). The diet enriched with both n-6 and n-3 PUFA resulted in the greatest increase in retinal DHA. The levels of PGE(1) and PGE(2) were significantly increased in retinal homogenates of rats fed with the GLA-rich diet when compared with those of animals fed the control diet. These higher PGE(1) and PGE(2) levels were not observed in animals fed with EPA + DHA + GLA. In summary, GLA added to EPA + DHA resulted in the highest retinal DHA content but without increasing retinal PGE(2) as seen in animals supplemented with GLA only.
authors.affiliation: Eye and Nutrition Research Group, INRA, UMR1129 FLAVIC, 21000, Dijon, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Schnebelen
authors.forename: Stéphane
authors.initials: S
authors.lastname: Grégoire
authors.forename: Bruno
authors.initials: B
authors.lastname: Pasquis
authors.forename: Corinne
authors.initials: C
authors.lastname: Joffre
authors.forename: Catherine P
authors.initials: CP
authors.lastname: Creuzot-Garcher
authors.forename: Alain M
authors.initials: AM
authors.lastname: Bron
authors.forename: Lionel
authors.initials: L
authors.lastname: Bretillon
authors.forename: Niyazi
authors.initials: N
authors.lastname: Acar
mesh_terms: D000527:Alprostadil / Q000032:analysis; D000818:Animals; D019587:Dietary Supplements; D015232:Dinoprostone / Q000032:analysis; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D043371:Fatty Acids, Omega-6 / Q000494:pharmacology*; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000378:metabolism*; D017965:gamma-Linolenic Acid / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D010743:Phospholipids; D004281:Docosahexaenoic Acids; D017965:gamma-Linolenic Acid; D000527:Alprostadil; D015232:Dinoprostone