title: Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China.
abstract: In the present study, a genotype 4 HEV strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in Shanghai Children's hospital. The full capsid protein gene (ORF2) sequence of this strain was determined by RT-PCR method. Sequence analysis based on the full ORF2 sequence indicated that this HEV strain shared the highest sequence identity (97.6%) with another human HEV strain isolated from a Japanese patient who was infected by genotype 4 HEV during traveling in Shanghai. Phylogenetic analysis showed that this genotype 4 HEV was phylogenetically far from the genotype 4 HEV strain that was commonly prevalent in Shanghai swine group, suggesting that this strain may not come from swine group and not involved in zoonotic transmission in this area.
authors.affiliation: Zoonosis and Comparative Medicine Group, School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai, PR China. z0216wen@sjtu.edu.cn
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Shixing
authors.initials: S
authors.lastname: Yang
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.forename: Junfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Tongling
authors.initials: T
authors.lastname: Shan
authors.forename: Fen
authors.initials: F
authors.lastname: Huang
authors.forename: Huibo
authors.initials: H
authors.lastname: Ning
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Kang
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
mesh_terms: D002681:China; D005243:Feces / Q000821:virology; D005838:Genotype; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: C400971:ORF2 protein, Hepatitis E virus; D014764:Viral Proteins