[Determination of mineral elements in brown rice of near-isogenic lines population for Japonica rice by ICP-AES].
In the present paper, the contents of 17 mineral elements (S, Mo, Ni, Fe, Cr, Na, Al, Cu, P, Sn, Zn, B, Mn, Mg, Ca, Sr and K) in brown rice of 264 plant lines in near-isogenic lines (NILs) population and parents for Japonica rice were determined by ICP-AES technique. The method proved to be simple, rapid, highly sensitive and accurate, and can be used to determine many elements at the same time. Its recovery ratio obtained by standard addition method ranged between 92.3% and 110.27%, and its RSD was lower than 6%. The analytical results showed that NILs for various mineral elements based on the BC5F5 population consisting of 261 lines were developed from a cross between NIL (BC4F5) and Towada. To our knowledge, this is the first report on that near-isogenic lines NIL(BC4F5) with the important value for high mineral elements associated with cold tolerance at booting stage have been bred. The results of the determination show that the brown rice is rich in eleven elements such as P, K, Mg, S, Ca, Zn, Mn, Na, Fe, Cu and Al necessary to human health. There is smaller variation of 5 macroelements (11.15%-16.45%) but larger variation of 12 microelements (16.57%-94.96%) in brown rice from populations. There is significant correlation among 95 from 136 pairs in 17 elements, moreover, especially the correlation (0.531**-0.921**) among 15 pairs of six micro-elements (Mo, Ni, Al, Sn, Cr and Sr) associated with the active components of functional rice is relatively higher than that of 10 pairs (0.175**-0.867**) of 5 macroelements (P, K, Ca, Mg and S), revealing the importance of microelements to catalysing and synthesizing the active components of functional rice. The above results provided reliable data and theory bases for gene location and cloning of controlling elements contents in brown rice, and further revealed the molecular and physiological mechanism of the relation between mineral elements in brown rice and cold tolerance at booting stage in japonica rice from Yunnan Province of China.
Zeng
Ya-wen
YW
Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China. zengyw1967@126.com
Wang
Lu-xiang
LX
Sun
Zheng-hai
ZH
Yang
Shu-ming
SM
Du
Juan
J
Li
Qi-wan
QW
Pu
Xiao-ying
XY
Du
Wei
W
Xiao
Feng-hui
FH
D008903:Minerals / Q000032:analysis* / Q000737:chemistry; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic
D008903:Minerals