Prognostic value of sperm fluorescence in situ hybridization analysis over PGD.
BACKGROUND
Sperm aneuploidy screening is now widely used as a counseling tool before performing a PGD cycle in infertile patients. The goal of this study was to determine whether sperm aneuploidy has a direct influence on embryo chromosomal abnormalities.


METHODS
Two groups were set up: (i) study group consisting of 13 oocyte-donation PGD cycles from males with normal karyotype and a previous altered sperm fluorescence in situ hybridization (FISH) result and (ii) control group including nine cycles of PGD for X-linked disease with no fertility problems and maternal age <36 years. Sperm samples and Day 3 embryos were evaluated using FISH for chromosomes X, Y, 13, 18 and 21.


RESULTS
A total of 179 embryos were analyzed: 91 embryos for the control group versus 88 for the study group. The study group presented more abnormal embryos than the control group (51.14% versus 35.16%; P = 0.0353). Patients from the study group were then classified according to sperm count. Oligozoospermic patients showed a much higher proportion of abnormal embryos compared with the control group (64.87% versus 35.16%; P = 0.0030).


CONCLUSIONS
Sperm aneuploidy and diploidy screening seems to be an effective prognostic tool that would be useful in the reproductive genetic counseling of infertile couples, especially in oligozoospermic patients.
Sánchez-Castro
M
M
Reprogenetics Spain, S.A., 08006 Barcelona, Spain.
Jiménez-Macedo
A R
AR
Sandalinas
M
M
Blanco
J
J
D000782:Aneuploidy; D002869:Chromosome Aberrations; D003376:Counseling; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000175:diagnosis*; D019836:Preimplantation Diagnosis; D011379:Prognosis; D012189:Retrospective Studies; D013094:Spermatozoa / Q000502:physiology*