Proliferation and gene expression of osteoblasts cultured in DMEM containing the ionic products of dicalcium silicate coating.
The medium containing ionic products of dicalcium silicate (Ca(2)SiO(4)) for culturing the osteoblast-like cells (MG63) were prepared by immersing the plasma sprayed Ca(2)SiO(4) coatings in DMEM solution for 24h. The normal DMEM was also used to culture the MG63 cells as the control group. The results obtained from this work showed that the proliferation of MG63 cell was more significant in the group containing ionic products of Ca(2)SiO(4) coatings than in the control group. The cell cycle distribution indicated that the decreased G(0)/G(1) phase and increased S phase occurred in the cells cultured in the DMEM containing ionic products of Ca(2)SiO(4) coatings. The analysis of osteogenic genes indicated that the ionic products of Ca(2)SiO(4) coating enhanced the expression of osteoblast-related genes and promote differentiation of MG63 cells at initial period. Moreover, the ionic products of Ca(2)SiO(4) coating differentially regulated osteoclastogenic gene expression by up-regulated OPG and down-regulated RANKL.
Sun
Junying
J
Orthopaedic Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.
Li
Jianyou
J
Liu
Xuanyong
X
Wei
Li
L
Wang
Guocheng
G
Meng
Fanhao
F
D000469:Alkaline Phosphatase / Q000235:genetics; D017610:Calcium Compounds / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics; D053244:Osteoprotegerin / Q000235:genetics; D053245:RANK Ligand / Q000235:genetics; D017640:Silicates / Q000494:pharmacology*
D017610:Calcium Compounds; D003470:Culture Media; D053244:Osteoprotegerin; D053245:RANK Ligand; D017640:Silicates; C505688:TNFSF11 protein, human; C013481:dicalcium silicate; D015675:Osteocalcin; D000469:Alkaline Phosphatase