[Experimental research on endocrine disturbing effect of fluorin on hypothalamus-hypophysis-testis axis in male rats].
OBJECTIVE
To study the endocrine disturbing effect of fluorin on Hypothalamus-Hypophysis-Testis axis in male rats.


METHODS
A total of 36 Wister male rats weighting 60-70 g were randomly divided into group I (high fluoride group of F-100 mg/l), group II (low fluoride group of F- 30 mg/l), group III (control group with pure water), with 12 rats in each group. Fluoride was administered with drinking water for 8 weeks. Then the level of procreation hormone in serum was detected by RIA method. And the spermatozoa quality was analysized.


RESULTS
There was difference between group I, group II and group III each other (P < 0.05) in body weight. As to right testis weight, there was difference between group I, group II and group III each other (P < 0.05). Epididymide organic coefficient in group II and group I were lower than that in group III (P > 0.05). Compared with group III, the counts amount of sperm and the rates of sperm mobility in group II and group I singnificantly increased (P < 0.05), and the rates of sperm aberration in group II and group I significantly decreased (P < 0.05), compared with group II, the sperm quality of group I descreased significantly (P < 0.05). The level of GnRH in three groups were significant difference between each groups (P <0.05). The level of FSH in three groups were significant difference between each groups (P < 0.05). The level of ICSH in three groups were no significant difference between each groups (P > 0.05). The level of T in Group I is significant lower than that of in Group II and Group III (P < 0.05). The level of E2 in Group I is significant higher than that of in Group II and Group III (P < 0.05).


CONCLUSIONS
These data support our hypothesis that fluorin have no significant effect on the function of male Rat Hypothalamus, that fluorin may effect the function of male Rat Hypophysis, that T/ICSH can be used to find the functional change of Leydig cells. Fluorin could affect hormone levels of each layer of the Hypothalamus-Hypophysis-Testis axis, and show the male reproductive endocrine disturbing effects.
Ma
Xiaoying
X
Department of Environmental Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Cheng
Xuemin
X
Li
Furan
F
Guo
Junsheng
J
D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity*; D005461:Fluorine / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000097:blood; D006062:Gonadotropins / Q000097:blood*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000166:cytology / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
D052244:Endocrine Disruptors; D006062:Gonadotropins; D005461:Fluorine; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone