Comparison of two combinations of cyanine dyes for prelabelling and gel electrophoresis.
We report the use of IC-OSu ethyl-Cy3 and ethyl-Cy5 N-hydroxysuccinimide ester (NHS) cyanine dyes, which have similar chemical properties as the CyDye DIGE fluor minimal dyes for pre-electrophoresis labelling. Multiple sample analyses in different laboratories indicate that the use of IC-OSu ethyl-Cy3 and ethyl-Cy5 NHS ester cyanine dyes produces equivalent results to those obtained with DIGE CyDyes, and allows sample multiplexing and accurate quantitation for differential proteome analysis.
Tsolakos
Nikos
N
Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK.
Techanukul
Tanasit
T
Wallington
Amanda
A
Zhao
Yuan
Y
Jones
Christopher
C
Nagy
Judit
J
Wheeler
Jun X
JX
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism*; D002457:Cell Extracts; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D051379:Mice; D011506:Proteins / Q000032:analysis; D012984:Software; D013194:Staining and Labeling / Q000379:methods*
D002232:Carbocyanines; D002457:Cell Extracts; D011506:Proteins