A novel dietary restriction method for group-housed rats: weight gain and clinical chemistry characterization.
Laboratory rodents are usually fed ad libitum. Moderate dietary restriction decreases mortality and morbidity compared with ad libitum feeding. There are, however, problems in achieving dietary restriction. Traditional methods of restricted feeding may interfere with the diurnal rhythms of the animals and are not compatible with group-housing of rodents. We have invented a novel method, the diet board, for restricting the feed intake of laboratory rats. The use of the diet board moderately decreased weight gain of rats when compared with ad libitum-fed animals. The diet board retarded skeletal growth only minimally, whereas major differences were found in body fat depositions. Serum free fatty acid, triglyceride and cholesterol values were lower in diet-restricted rats, while the opposite was true for serum creatine kinase. There were no differences in total protein, albumin or alanine aminotransferase. Moreover, differences in interindividual variances in parameters were not detected between the groups; hence this study could not combine the diet board with reduction potential. The diet board provides mild to moderate dietary restriction for group-housed rats and is unlikely to interfere with the diurnal eating rhythm. The diet board can also be seen as a cage furniture item, dividing the open cage space and increasing the structural complexity of the environment. In conclusion, the diet board appears to possess refinement potential when compared with traditional methods of dietary restriction.
Kasanen
I H E
IH
National Laboratory Animal Center, University of Kuopio, PO Box 1627, FI-70211, Kuopio, Finland. Iiris.Kasanen@uku.fi
Inhilä
K J
KJ
Nevalainen
J I
JI
Väisänen
S B
SB
Mertanen
A M O
AM
Mering
S M
SM
Nevalainen
T O
TO
D000410:Alanine Transaminase / Q000097:blood; D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D000830:Animals, Laboratory / Q000254:growth & development* / Q000378:metabolism; D001823:Body Composition / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D015331:Cohort Studies; D003402:Creatine Kinase / Q000097:blood; D004435:Eating; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005508:Food Deprivation; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar / Q000254:growth & development* / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain
D005230:Fatty Acids, Nonesterified; D012709:Serum Albumin; D014280:Triglycerides; D002784:Cholesterol; D000410:Alanine Transaminase; D003402:Creatine Kinase