title: Vascular endothelial growth factor in allergen-induced nasal inflammation.
abstract: BACKGROUND
  Increased vessel number and permeability are important features of the nasal mucosa in allergic rhinitis (AR), and are mediated in part by the cytokine vascular endothelial growth factor (VEGF). Eosinophils are the major effector cells in the nasal secretions of patients with AR during the responses to allergen challenges. To evaluate the involvement of VEGF in nasal allergic inflammation, we monitored the levels of VEGF, eosinophil cationic protein (ECP), and specific antibodies in the nasal lavage fluids (NLFs) of patients with AR in response to Dermatophagoides pteronyssinus (Dpt).
  
  
  METHODS
  Sixty-three subjects with sensitization to Dpt were enrolled: 29 patients with AR (group I) who showed positive responses in a nasal provocation test (NPT) with Dpt; and 34 asymptomatic controls (group II) who showed sensitization to Dpt but negative NPT results. NLF samples were collected at baseline, 10, 30, and 60 min, and at 3, 6, and 24 h during the NPT. The ECP levels in the NLF samples were measured using the ImmunoCAP system. VEGF and Dpt-specific IgE, IgA, and IgG in the NLF samples were detected by ELISA.
  
  
  RESULTS
  The eosinophil counts and ECP levels in the samples were significantly increased in group I, but not in group II, during the early and late responses. Although the baseline VEGF level was not significantly different between groups I and II, increased VEGF production was noted in group I after the NPT, especially during the early response. The level of Dpt-specific IgA was significantly increased in group I during the NPT. A relationship was found between the levels of VEGF and ECP or Dpt-specific IgA in the NLF samples collected at 10 min and at 3-6 h (P<0.05, respectively).
  
  
  CONCLUSIONS
  Nasal VEGF secretion in response to allergen exposure may augment eosinophilic inflammation in the nasal mucosa of patients with AR.
authors.affiliation: Department of Allergy and Rheumatology, Ajou University School of Medicine, Youngtong-gu, Suwon, Korea.
authors.forename: G-S
authors.initials: GS
authors.lastname: Choi
authors.forename: H-J
authors.initials: HJ
authors.lastname: Park
authors.forename: G-Y
authors.initials: GY
authors.lastname: Hur
authors.forename: S-J
authors.initials: SJ
authors.lastname: Choi
authors.forename: S-Y
authors.initials: SY
authors.lastname: Shin
authors.forename: Y-M
authors.initials: YM
authors.lastname: Ye
authors.forename: H-S
authors.initials: HS
authors.lastname: Park
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D047150:Eosinophil Cationic Protein / Q000276:immunology / Q000378:metabolism*; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008297:Male; D017817:Nasal Lavage Fluid / Q000276:immunology; D009299:Nasal Provocation Tests; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology* / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology / Q000378:metabolism*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D042461:Vascular Endothelial Growth Factor A; D007073:Immunoglobulin E; D047150:Eosinophil Cationic Protein; C479220:RNASE3 protein, human