Detection of apoptosis based on the interaction between annexin V and phosphatidylserine.
The detection of apoptosis based on the interaction between annexin V and phosphatidylserine is presented in this paper. The in vitro experimental results demonstrate that annexin V in polyethylenimine film can maintain its high affinity with phosphatidylserine translocated from the inner to the outer plasma membrane of the apoptotic cells, which may consequently inhibit the redox reaction of [Ru(NH(3))(5)Cl](2+/+) at the annexin V and polyethylenimine comodified pyrolytic graphite electrode. Therefore, if Ca(2+), the required ion for the function of annexin V, is added into the test solution, the redox wave of [Ru(NH(3))(5)Cl](2+/+) can be clearly changed to indicate apoptosis and a simple and convenient method to detect apoptosis is developed.
Liu
Ting
T
Zhu
Wei
W
Yang
Xiang
X
Chen
Lin
L
Yang
Rongwu
R
Hua
Zichun
Z
Li
Genxi
G
D017304:Annexin A5 / Q000378:metabolism*; D017209:Apoptosis; D002118:Calcium / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D004566:Electrodes; D006801:Humans; D010084:Oxidation-Reduction; D010718:Phosphatidylserines / Q000378:metabolism*; D011094:Polyethyleneimine / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry
D017304:Annexin A5; D010718:Phosphatidylserines; D002244:Carbon; D012428:Ruthenium; D011094:Polyethyleneimine; D002118:Calcium