Genetic evidence for CheB- and CheR-dependent chemotaxis system in A. tumefaciens toward acetosyringone.
Agrobacterium tumefaciens has homologues of chemotaxis genes of the alpha-subgroup of proteobacteria, which include orf1, orf2, cheY1, cheA, cheR, cheB, cheY2, orf9, and orf10 and organized in one operon [Wright et al. A chemotaxis cluster from Agrobacterium tumefaciens. Gene 1998;220:83-9]. Two mutants of A. tumefaciens were created by in-frame deletions of cheB and cheR using suicide vector, pK18mobsacB. The chemosensory behaviour of mutants studied on swarm plates and in blind-well chemotaxis assay toward acetosyringone. Under the conditions tested, both CheR and CheB were essential for normal chemotaxis.
Harighi
Behrouz
B
Department of Plant Protection, College of Agriculture, University of Kurdistan, Sanandaj, Iran. BHarighi@uok.ac.ir
D000098:Acetophenones / Q000494:pharmacology*; D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion
D000098:Acetophenones; D001426:Bacterial Proteins; C069656:CheB protein, Bacteria; C051667:acetosyringone; D008780:Methyltransferases