title: Acute toxicity of ochratoxin-A in marine water-reared sea bass (Dicentrarchus labrax L.).
abstract: The toxic effects of ochratoxin-A (OTA) on sea bass, Dicentrarchus labrax L., have not been previously documented. A flow-through bioassay test system was conducted in two series and a total 180 of adult marine-reared sea bass was used to estimate the acute oral 96 h median lethal concentration (LC(50)) value and behavioral changes of OTA. The data obtained were statistically evaluated using Finney's Probit Analysis Method developed by EPA. The 96 h LC(50) value for adult D. labrax was found to be 277 microg kg(-1)bwt with 95% confidence limits of 244-311 microg kg(-1)bwt. This value was calculated to be 285 microg kg(-1) bwt with Behrens-Karber's method. The two methods were relatively comparable. The acute dietary 96 h LC(50) of OTA is 9.23 mg kg(-1) diet. Additionally, the behavioral changes of sea bass were primarily observed as nervous and respiratory manifestations. We concluded that sea bass is a species highly sensitive to OTA making them a useful experimental model for aquatic mycotoxigenic problems.
authors.affiliation: Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Alexandria University, Edfina, Behera Province, Egypt. yasser_tf@yahoo.com
authors.forename: Yasser Said
authors.initials: YS
authors.lastname: El-Sayed
authors.forename: Riad Hassan
authors.initials: RH
authors.lastname: Khalil
authors.forename: Talaat Talaat
authors.initials: TT
authors.lastname: Saad
mesh_terms: D000821:Animal Feed; D000818:Animals; D001492:Bass / Q000378:metabolism / Q000502:physiology*; D001522:Behavior, Animal; D017753:Ecosystem; D007928:Lethal Dose 50; D009793:Ochratoxins / Q000494:pharmacology / Q000633:toxicity*; D014873:Water Pollutants / Q000494:pharmacology / Q000633:toxicity*
chemical_list: D009793:Ochratoxins; D014873:Water Pollutants; C025589:ochratoxin A