[Expression of WAVE1 and p22phox in children with acute lymphocytic leukemia and the relationship of WAVE1 with oxidative stress].
OBJECTIVE
To study the expression of WAVE1 and p22phox in peripheral blood mononuclear cells (PBMCs) in children with acute lymphocytic leukemia (ALL) and the relationship of WAVE1 with oxidative stress.


METHODS
Real-time PCR was used for detecting WAVE1 and p22phox expression in PBMCs in 41 children with ALL and 10 normal controls. Plasma activity of superoxide dismutase (SOD) was measured by the xanthine oxidase method. Plasma activity of GSH-Px was measured by the DTNB reaction test.


RESULTS
The expression of WAVE1 and p22phox was significantly higher in the active ALL groups (newly diagnosed and relapse ALL) than that in the normal control and the complete remission (CR) ALL groups (<0.01). The CR ALL group showed increased WAVE1 and p22phox expression than those in the normal control group (<0.05). Plasma activities of SOD (22.62+/-7.39 U/mL) and GSH-Px (91.73+/-28.88 micromol/L) in the active ALL group were significantly lower than those in the normal control (166.35+/-27.93 U/mL and 490.94+/-39.38 micromol/L, respectively) and the CR ALL groups (107.11+/-28.57 U/mL and 267.56+/-82.64 micromol/L, respectively) (<0.01). WAVE1 expression was positively correlated with p22phox expression (r=0.34, <0.05) but negatively correlated with plasma activities of SOD and GSH-Px ( r=-0.336 and-0.408, respectively; <0.05).


CONCLUSIONS
WAVE1 and p22phox expression in PBMCs increased and was associated with the disease course in children with ALL. Oxidative stress may be involved in the regulation of WAVE1 expression in ALL children.
He
Yu-Lei
YL
Department of Pediatrics, Xiangya Hospital of Central South University, Changsha 410008, China.
Cao
Li-Zhi
LZ
Yang
Jing
J
Yang
Ming-Hua
MH
Xu
Wang-Qiong
WQ
Xie
Min
M
Shi
Zhuang
Z
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D019255:NADPH Oxidases / Q000235:genetics*; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000235:genetics*
D012333:RNA, Messenger; C114864:WASF1 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; C086986:CYBA protein, human