title: Fungal transmission of plant viruses.
abstract: Fungal zoospores of Olpidium species transmit several viruses in the family Tombusviridae as well as in the Ophio- and Varicosavirus genera. This unit describes procedures for virus transmission by Olpidium sp. The method is useful for assessing fungal transmissibility of a given virus as well as for further studies on molecular and biological aspects of virus/vector interaction.
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, British Columbia, Canada.
authors.forename: D'Ann
authors.initials: D
authors.lastname: Rochon
mesh_terms: D008411:Chytridiomycota / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology / Q000821:virology*; D018553:Cucumis sativus / Q000382:microbiology; D003470:Culture Media; D018545:Lactuca / Q000382:microbiology; D008828:Microbiological Techniques; D010935:Plant Diseases / Q000821:virology*; D018517:Plant Roots / Q000382:microbiology; D010942:Plant Viruses / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D012328:RNA Viruses / Q000502:physiology*; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000821:virology*; D019183:Tombusviridae / Q000502:physiology
chemical_list: D003470:Culture Media