title: beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio).
abstract: The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5 mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5 mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5 mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1 beta but seemed to modulate IFNgamma and chemokine expression in kidney.
authors.affiliation: Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas (CSIC), Eduardo Cabello 6, Vigo, Spain.
authors.forename: Iván
authors.initials: I
authors.lastname: Rodríguez
authors.forename: Rubén
authors.initials: R
authors.lastname: Chamorro
authors.forename: Beatriz
authors.initials: B
authors.lastname: Novoa
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueras
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D016207:Cytokines / Q000097:blood; D005393:Fish Diseases / Q000276:immunology* / Q000401:mortality; D005786:Gene Expression Regulation / Q000187:drug effects; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D007113:Immunity, Innate / Q000187:drug effects*; D007668:Kidney / Q000166:cytology; D016019:Survival Analysis; D015027:Zebrafish / Q000276:immunology*; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D047071:beta-Glucans