title: Mini sensing chip for point-of-care acute myocardial infarction diagnosis utilizing micro-electro-mechanical system and nano-technology.
abstract: A rapid and accurate diagnosis of acute myocardial infarction (AMI) is crucial for saving lives. For this purpose, we have been developing a rapid, automatic, point-of-care, biosensing system for simultaneous four cardiac marker quantification. This system performs a fluorophore mediated immuno-sensing on optical fibers. To improve the sensitivity of the sensor, novel nanoparticle reagents enhancing fluorescence were implemented. Micro-electro-mechanical system (MEMS) technology was applied in the sensing chip development and automatic sensing operation was implemented to ensure a reliable and user-friendly assay. The resulting system is a point-of-care, automatic four cardiac marker sensing system with a 2 x 2.5 cm sensing chip. An assay requires a 200 microL plasma sample and 15-minute assay time.
authors.affiliation: Department of Chemical Engineering, University of Louisville, Louisville, KY 40292, USA.
authors.forename: Jianting
authors.initials: J
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Hong
authors.forename: Junhai
authors.initials: J
authors.lastname: Kai
authors.forename: Jungyoup
authors.initials: J
authors.lastname: Han
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zou
authors.forename: Chong H
authors.initials: CH
authors.lastname: Ahn
authors.forename: Kyung A
authors.initials: KA
authors.lastname: Kang
mesh_terms: D000208:Acute Disease; D015415:Biomarkers; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation* / Q000379:methods*; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D020097:Natriuretic Peptide, Brain / Q000032:analysis
chemical_list: D015415:Biomarkers; D020097:Natriuretic Peptide, Brain