The first complete genome sequence of a chicken group A rotavirus indicates independent evolution of mammalian and avian strains.
Group A rotaviruses are a leading cause of gastroenteritis in humans and animals. Transmission between mammalian species and humans has been demonstrated repeatedly. Here, the first entire genome sequence (19,064 bp) of a chicken rotavirus, strain Ch-02V0002G3, is presented. A low degree of nucleotide sequence identity with the mammalian group A rotaviruses is evident for all 11 genome segments, whereas a closer relationship to available rotavirus sequences from avian species has been determined. According to a novel rotavirus classification system, new genotypes were proposed and ratified by the Rotavirus Classification Working Group for eight of the Ch-02V0002G3 genome segments, resulting in the genotype constellation G19-P[30]-I11-R6-C6-M7-A16-N6-T8-E10-H8. Due to the low percentages of genome sequence identity, the different genome segment sizes and the marked sequence differences of non-structural proteins, an independent evolution without exchange of genetic material between mammalian and avian group A rotavirus strains is likely.
Trojnar
Eva
E
Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
Otto
Peter
P
Johne
Reimar
R
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000821:virology*; D019143:Evolution, Molecular; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA
D012367:RNA, Viral