Magnetic microbead-based electrochemical immunoassays.
This review provides a summary of recent works concerning electrochemical immunoassays using magnetic microbeads as a solid phase. Recent research activity has led to innovative and powerful detection strategies that have been resulted in sensitive electrochemical detection. Coupling of magnetic microbeads with highly sensitive electrochemical detection provides a useful analytical method for environmental evaluation and clinical diagnostics, etc. The huge surface area and high dispersion capability of magnetic microbeads strongly contributes towards the development of new sensitive, rapid, user-friendly, and miniaturized electrochemical immunoassay systems. Moreover, the immunocomplexes formed on the magnetic microbead surface can be easily detected without pretreatment steps such as preconcentration or purification, which are normally required for standard methods. The discussion in this review is organized in two main subjects that include magnetic-microbead-based assays using enzyme labels and nanoparticle tags.
Kuramitz
Hideki
H
Department of Environmental Biology and Chemistry, Graduate School of Science and Engineering for Research, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan. kuramitz@sci.u-toyama.ac.jp
D004563:Electrochemistry / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008280:Magnetics; D008863:Microspheres; D012680:Sensitivity and Specificity; D013499:Surface Properties