title: Composition of transgenic Volvariella volvacea tolerant to cold stress is equivalent to that of conventional control.
abstract: Transgenic Volvariella volvacea strains, which are tolerant to cold stress, were generated by the stable insertion of antifreeze protein gene isolated from budworm into the genome of a conventional variety V23 of V. volvacea. As a part of the safety assessment program, transgenic V. volvacea strains were compared to a nontransgenic near-isogenic V. volvacea strain V23 grown contemporaneously by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring proximates, amino acids, fatty acids, minerals, vitamins, 5'-nucleotides, nucleic acid, and antinutrients such as tannin and cyanide in V. volvacea strains harvested at egg-shaped stage. Results of the comparisons indicate that these transgenic strains are compositionally equivalent to the conventional control.
authors.affiliation: Department of Bioengineering, College of Food Science, and Institute of Biomass Research, South China Agricultural University, Guangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Liqiong
authors.initials: L
authors.lastname: Guo
authors.forename: Junfang
authors.initials: J
authors.lastname: Lin
mesh_terms: D000596:Amino Acids / Q000032:analysis; D021301:Antifreeze Proteins / Q000235:genetics; D003080:Cold Temperature; D005227:Fatty Acids / Q000032:analysis; D008903:Minerals / Q000032:analysis; D009711:Nucleotides / Q000032:analysis; D030781:Organisms, Genetically Modified; D014815:Vitamins / Q000032:analysis; D055367:Volvariella / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D021301:Antifreeze Proteins; D005227:Fatty Acids; D008903:Minerals; D009711:Nucleotides; D014815:Vitamins