Diversity of culturable bacteria isolated from root domains of moso bamboo (Phyllostachys edulis).
The distribution of culturable bacteria in the rhizosphere, rhizoplane, and interior root tissues of moso bamboo plants was investigated in this study. Of the 182 isolates showing different colony characteristics on Luria-Bertani and King B plates, 56 operational taxonomic units of 22 genera were identified by 16S ribosomal RNA gene sequence analysis. The majority of root endophytic bacteria were Proteobacteria (67.5%), while the majority of rhizospheric and rhizoplane bacteria were Firmicutes (66.3% and 70.4%, respectively). The most common genus in both the rhizosphere and on the rhizoplane was Bacillus (42.4% and 44.4%, respectively), while Burkholderia was the most common genus inside the roots, comprising 35.0% of the isolates from this root domain. The endophytic bacterial community was less diverse than the rhizoplane and rhizospheric bacterial communities. Members of Lysinibacillus, Bacillus, and Burkholderia were found in all three root domains, whereas many isolates were found in only a single domain. Our results show that the population diversity of culturable bacteria is abundant in the root domains of moso bamboo plants and that obvious differences exist among the rhizospheric, rhizoplane, and endophytic bacterial communities.
Han
Jigang
J
Research Center of Biological Engineering for Hebei Province, College of life Sciences, Hebei University, Baoding 071002, China. jghan@hbu.edu.cn
Xia
Dongliang
D
Li
Lubin
L
Sun
Lei
L
Yang
Kai
K
Zhang
Liping
L
D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D044822:Biodiversity; D002681:China; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D006109:Poaceae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S