title: Induced sputum eosinophilia in ulcerative colitis patients: the lung as a mirror image of intestine?
abstract: BACKGROUND
  Ulcerative colitis (UC) is a systemic disease of unknown etiology with extra-intestinal manifestation. Induced sputum (IS) non-invasively assesses extrapulmonary involvement in Crohn's disease. We sought to determine whether there is a cellular marker of lung injury in UC patients detectable by IS.
  
  
  METHODS
  Nineteen UC patients (mean age 46.4+/-11.3 years, disease duration 8.6+/-7.5 years [range 1-25 years] 68.4% males) were studied, 6 with active disease and 13 in remission. Eleven received 5-ASA, 5 received steroids and/or azathioprine and 3 patients were untreated. UC patients were compared with 27 healthy non-smoker controls. IS was recovered after 20 min inhalation of 3% saline with an ultrasonic nebulizer by the selecting plugs method, and 300 cells were differentially cell counted in cytospin Giemsa-stained slides. CD4/CD8 subsets were identified by FACS. Pulmonary function tests were performed by the Jaeger Masterlab spirometer.
  
  
  RESULTS
  UC patients' IS contained higher %eosinophils than controls (p=0.05) and lower FEV(1)/FVC ratios (p=0.001). Steroid- and/or azathioprine-treated patients had significantly lower FEV(1)/FVC ratios than only 5-ASA-treated patients (p=0.019). Eosinophil infiltration in airways was high in 5-ASA-treated patients compared to those receiving steroids and/or azathioprine (p=0.046) and those with less extensive disease (p=0.05). Using a cutoff of 3% eosinophils, IS had a sensitivity of 67% and specificity of 73% to differentiate patients with a cutoff of 70 eosinophils/mm(2) in biopsy.
  
  
  CONCLUSIONS
  The percentage of sputum eosinophils is significantly different between UC patients with proctitis and pancolitis. These immune abnormalities may be a common pattern that is present throughout the mucosae.
authors.affiliation: Department of Pulmonary and Allergic Diseases, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Fireman
authors.forename: Farid
authors.initials: F
authors.lastname: Masarwy
authors.forename: Gabi
authors.initials: G
authors.lastname: Groisman
authors.forename: Moshe
authors.initials: M
authors.lastname: Shtark
authors.forename: Yael
authors.initials: Y
authors.lastname: Kopelman
authors.forename: Shmuel
authors.initials: S
authors.lastname: Kivity
authors.forename: Zvi
authors.initials: Z
authors.lastname: Fireman
mesh_terms: D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D004804:Eosinophils / Q000166:cytology*; D005260:Female; D006801:Humans; D007422:Intestines / Q000276:immunology* / Q000473:pathology; D008168:Lung / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012129:Respiratory Function Tests; D013183:Sputum / Q000166:cytology*; D016176:T-Lymphocyte Subsets / Q000166:cytology*