title: Promotion of nano-anatase TiO(2) on the spectral responses and photochemical activities of D1/D2/Cyt b559 complex of spinach.
abstract: Previous researches approved that photocatalysis activity of nano-TiO(2) could obviously increase photosynthetic effects of spinach, but the mechanism of improving light energy transfer and conversion is still unclear. In the present we investigated effects of nano-anatase TiO(2) on the spectral responses and photochemical activities of D1/D2/Cyt b559 complex of spinach. Several effects of nano-anatase TiO(2) were observed: (1) UV-vis spectrum was blue shifted in both Soret and Q bands, and the absorption intensity was obviously increased; (2) resonance Raman spectrum showed four main peaks, which are ascribed to carotene, and the Raman peak intensity was as 6.98 times as that of the control; (3) the fluorescence emission peak was blue shifted and the intensity was decreased by 23.59%; (4) the DCPIP photoreduction activity showed 129.24% enhancement; (5) the oxygen-evolving rate of PS II was elevated by 51.89%. Taken together, the studies of the experiments showed that nano-anatase TiO(2) had bound to D1/D2/Cyt b559 complex, promoted the spectral responses, leading to the improvement of primary electron separation, electron transfer and light energy conversion of D1/D2/Cyt b559 complex.
authors.affiliation: Medical College of Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Su
authors.forename: Huiting
authors.initials: H
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Qu
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D003573:Cytochrome b Group / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism; D018724:Spinacia oleracea / Q000378:metabolism*; D014025:Titanium / Q000737:chemistry*
chemical_list: D003573:Cytochrome b Group; D017319:Photosensitizing Agents; D045332:Photosystem II Protein Complex; C009495:titanium dioxide; C019313:cytochrome b559; D014025:Titanium