Comparative studies on the interaction between bovine beta-lacto-globulin type A and B and a new designed Pd(II) complex with anti-tumor activity at different temperatures.
An new water-soluble Pd(II) complex, 2,2'-bipyridin n-butyl dithiocarbamato Pd(II) nitrate has been synthesized. The Pd(II) complex has been characterized by elemental analysis and conductivity measurements as well as spectroscopic methods such as infrared, 1H NMR, and ultraviolet-visible. The interaction between this new design Pd(II)-complex, an anti-tumor component, with carrier proteins of beta-lactoglobulin-A and -B (BLG-A and -B) were studied at different temperatures of 27, 37, 42, and 47 degrees C by fluorescence spectroscopy and far-UV circular dichroism (CD) spectrophotometric techniques. A strong fluorescence quenching interaction of Pd(II) complex with BLG-A and -B was observed at different temperatures. The binding parameters were evaluated by fluorescence quenching method. The thermodynamic parameters, including DeltaH degrees , DeltaS degrees , and DeltaG degrees were calculated by fluorescence quenching method indicated that the electrostatic and hydrophobic forces might play a major role in the interactions of Pd(II) complex with BLG-A and -B, respectively. The distances between donors (Trps of the BLG-A and -B) and acceptor (Pd(II) complex) were obtained according to the fluorescence resonance energy transfer (FRET). Far-UV CD studies showed that the Pd(II) complex did not represent any significant changes in the secondary structures of BLG-A and -B. The difference in the interaction properties observed for BLG-A and -B with Pd(II) complex is related to the difference in the amino acid sequences between these two variants.
Divsalar
Adeleh
A
Institute of Biochemistry and Biophysics, University of Tehran, Iran. divsalar@ibb.ut.ac.ir
Saboury
Ali Akbar
AA
Mansouri-Torshizi
Hassan
H
Moghaddam
Mahboube Islami
MI
Ahmad
Faizan
F
Hakimelahi
Golam Hossein
GH
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D015195:Drug Design; D031541:Fluorescence Resonance Energy Transfer; D007782:Lactoglobulins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000378:metabolism*; D010165:Palladium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013696:Temperature; D013816:Thermodynamics
C541236:2,2'-bipyridin n-butyl dithiocarbamato Pd(II) nitrate; D000970:Antineoplastic Agents; D007782:Lactoglobulins; D009942:Organometallic Compounds; D010165:Palladium