Effects of anesthesia on brain mitochondrial function, blood flow, ionic and electrical activity monitored in vivo.
Thiopental, a well-known barbiturate, is often used in patients who are at high risk of developing cerebral ischemia, especially during brain surgery. Although barbiturates are known to affect a variety of processes in the cerebral cortex, including oxygen consumption by the mitochondria, the interrelation between mitochondrial function and anesthetics has not been investigated in detail under in vivo conditions. The aim of this study was to examine the effects of thiopental on brain functions in normoxia and under partial or complete ischemia. The use of the multiparametric monitoring system permitted simultaneous measurements of microcirculatory blood flow, NADH fluorescence, tissue reflectance, and ionic and electrical activities of the cerebral cortex. Thiopental caused a significant, dose-dependent decrease in blood flow and a significant decrease in extracellular levels of potassium, with no significant changes in NADH levels in normoxic and ischemic rats. Following complete ischemia (death), the increase in the reflectance was significantly smaller in the anesthetized normoxic group versus the awake normoxic group. The time until the secondary increase in reflectance, seen in death, was significantly shorter in the anesthetized ischemic group. In conclusion, it seems that the protective effect of thiopental occurs only under partial ischemia and not under complete ischemia.
Dekel
Nava
N
The Mina & Everard Goodman Faculty of life sciences, Bar-Ilan University, Ramat-Gan, Israel.
Sonn
Judith
J
Barbiro-Michaely
Efrat
E
Ornstein
Eugene
E
Mayevsky
Avraham
A
D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D001923:Brain Chemistry; D004594:Electrophysiology; D007477:Ions / Q000737:chemistry; D008297:Male; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D007477:Ions