title: Immuno-MALDI-TOF MS: new perspectives for clinical applications of mass spectrometry.
abstract: The discovery of novel biomarkers by means of advanced detection tools based on proteomic analysis technologies necessitates the development of improved diagnostic methods for application in clinical routine. On the basis of three different application examples, this review presents the limitations of conventional routine diagnostic assays and illustrates the advantages of immunoaffinity enrichment combined with MALDI-TOF MS. Applying this approach increases the specificity of the analysis supporting a better diagnostic recognition, sensitivity, and differentiation of certain diseases. The use of MALDI-TOF MS as detection method facilitates the identification of modified peptides and proteins providing additional information. Further, employing respective internal standard peptides allows for relative and absolute quantitation which is mandatory in the clinical context. Although MALDI-TOF MS is not yet established for clinical routine diagnostics this technology has a high potential for improvement of clinical diagnostics and monitoring therapeutic efficacy.
authors.affiliation: Bruker Daltonik GmbH, Leipzig, Germany. ksp@bdal.de
authors.forename: Katrin
authors.initials: K
authors.lastname: Sparbier
authors.forename: Thomas
authors.initials: T
authors.lastname: Wenzel
authors.forename: Hassan
authors.initials: H
authors.lastname: Dihazi
authors.forename: Sabine
authors.initials: S
authors.lastname: Blaschke
authors.forename: Gerhard-Anton
authors.initials: GA
authors.lastname: Müller
authors.forename: André
authors.initials: A
authors.lastname: Deelder
authors.forename: Thomas
authors.initials: T
authors.lastname: Flad
authors.forename: Markus
authors.initials: M
authors.lastname: Kostrzewa
mesh_terms: D000818:Animals; D002624:Chemistry, Clinical / Q000379:methods*; D006801:Humans; D007120:Immunochemistry / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization