Efficient and chemoselective surface immobilization of proteins by using aniline-catalyzed oxime chemistry.
Site-specific immobilization of peptides and proteins is crucial to ensure their functionality in surface-based assays. We report the use of aniline-catalyzed oxime ligations as a very efficient and broadly applicable method to covalently attach the N terminus of proteins and peptides to a surface functionalized with alkoxy-amine groups.
Lempens
Edith H M
EH
Laboratory of Molecular Science and Technology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands.
Helms
Brett A
BA
Merkx
Maarten
M
Meijer
E W
EW
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D002384:Catalysis; D055767:Immobilized Proteins / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D013379:Substrate Specificity
D000814:Aniline Compounds; D055767:Immobilized Proteins; D010091:Oximes; D010455:Peptides; C023650:aniline