Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets.
Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
Joensson
Haakan N
HN
Department of Nano Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden. hakan.jonsson@biotech.kth.se
Samuels
Michael L
ML
Brouzes
Eric R
ER
Medkova
Martina
M
Uhlén
Mathias
M
Link
Darren R
DR
Andersson-Svahn
Helene
H
D018941:Antigens, CD19 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D005453:Fluorescence; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D019713:Receptors, CCR5 / Q000032:analysis; D020298:U937 Cells
D018941:Antigens, CD19; D015415:Biomarkers; D008565:Membrane Proteins; D019713:Receptors, CCR5