title: Prediction of preeclampsia by analysis of cell-free messenger RNA in maternal plasma.
abstract: OBJECTIVE
  The purpose of this study was to predict the occurrence of preeclampsia in a series of patients at gestational week 15-20 weeks, with the use of a panel of messenger RNA markers.
  
  
  METHODS
  Data from 62 patients with preeclampsia who were asymptomatic at the time of blood testing and 310 control subjects were analyzed. Multivariable analysis was performed with discriminant analysis.
  
  
  RESULTS
  Univariable analysis identified vascular endothelial growth factor receptor 1 as the marker with the highest detection rate; placenta-specific 1 with the lowest. Mean estimated score for preeclampsia was 9.4 for control subjects and 72.5 for subjects who experienced preeclampsia. A receiver operating characteristic curve that was obtained with the estimated score for preeclampsia as a test variable yielded a detection rate of 84% (95% CI, 71.8-91.5) at a 5% false-positive rate with an area under the curve of 0.927 (P < .001). Again, detection rate and score for each patient for classification as preeclamptic correlated with severity.
  
  
  CONCLUSIONS
  A panel of messenger RNA is able to detect subjects who will experience preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Yuditiya
authors.initials: Y
authors.lastname: Purwosunu
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
authors.forename: Shiho
authors.initials: S
authors.lastname: Okazaki
authors.forename: Antonio
authors.initials: A
authors.lastname: Farina
authors.forename: Noroyono
authors.initials: N
authors.lastname: Wibowo
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Nicola
authors.initials: N
authors.lastname: Rizzo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saito
authors.forename: Takashi
authors.initials: T
authors.lastname: Okai
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011446:Prospective Studies; D012333:RNA, Messenger / Q000097:blood*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger