title: Heparin-like effect contributes to the coagulopathy in patients with acute liver failure undergoing liver transplantation.
abstract: BACKGROUND
  Liver transplantation (LT) in cirrhotics is characterized by severe coagulopathy, associated with a well documented heparin-like effect (HLE) seen by thromboelastography (TEG) after reperfusion. The amount of HLE present in patients with acute liver failure (ALF) and its role in their bleeding tendency before LT has not been investigated.
  
  
  OBJECTIVE
  To investigate the presence and extent of HLE in patients with ALF undergoing LT and to compare the extent of HLE in this group with a group of cirrhotics undergoing LT.
  
  
  METHODS
  Ten consecutive ALF and 10 cirrhotic patients undergoing LT were included. TEG (with and without heparinase I), surrogate total thrombin generation (TTG) derived by TEG and haemodynamic variables were recorded for every stage of the LT. HLE was defined as a correction of r+k times on TEG of more than 50% by the addition of heparinase I.
  
  
  RESULTS
  Before incision, patients with ALF showed a significantly greater HLE compared with patients with cirrhosis (r+k time: 66 min corrected to 29 vs 45 min corrected to 32 min, P=0.001). After reperfusion, all the patients showed extensive HLE, without any difference between the two groups. Despite the greater HLE, patients with ALF showed similar TTG compared with the cirrhotic group. By the end of the operation, the extent of the HLE was greatly reduced in both the groups.
  
  
  CONCLUSIONS
  Before transplantation, patients with ALF have a greater HLE than patients with liver cirrhosis. However, this did not affect the thrombin generation calculated by TEG and resolved after transplantation.
authors.affiliation: Department of Surgical and Gastroenterological Sciences, University Hospital of Padua, Padua, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Senzolo
authors.forename: Seema
authors.initials: S
authors.lastname: Agarwal
authors.forename: Paola
authors.initials: P
authors.lastname: Zappoli
authors.forename: Sushang
authors.initials: S
authors.lastname: Vibhakorn
authors.forename: Susan
authors.initials: S
authors.lastname: Mallett
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Burroughs
mesh_terms: D000328:Adult; D001778:Blood Coagulation Disorders / Q000209:etiology* / Q000503:physiopathology; D005260:Female; D019763:Heparin Lyase; D006801:Humans; D017114:Liver Failure, Acute / Q000503:physiopathology / Q000601:surgery*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D013916:Thrombelastography / Q000379:methods*; D013917:Thrombin / Q000096:biosynthesis
chemical_list: D013917:Thrombin; D019763:Heparin Lyase