title: Genetic polymorphisms modifying oxidative stress are associated with disease activity in rheumatoid arthritis patients.
abstract: Reactive oxygen and nitrogen species are involved in the pathology of rheumatoid arthritis (RA). Polymorphisms in genes coding for superoxide dismutases (SOD2 and SOD3), catalase (CAT), tumor necrosis factor-alpha (TNFA) and inducible NO synthase (NOS2A) may influence RA activity. We determined SOD2 Ala-9Val, SOD3 Arg213Gly, CAT C-262T, TNFA G-308A, TNFA C-857T and NOS2A (CCTTT) (n)polymorphisms in 327 RA patients. Carriers of CAT -262T and TNFA -308A allele had lower mean disease activity score of 28 joint count (DAS28) values than patients with CAT -262CC and TNFA -308GG genotypes (p = 0.014 and p = 0.046, respectively). Patients with the combination of CAT -262T and TNFA -308A allele had lower mean DAS28 values and a higher probability for low disease activity than non-carriers (p = 0.003, OR = 3.585, 95% CI = 1.538-8.357). Our results suggest that CAT and TNFA polymorphisms alone and in combination influence the activity of RA.
authors.affiliation: University of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Ljubljana, Slovenia.
authors.forename: Petra
authors.initials: P
authors.lastname: Bohanec Grabar
authors.forename: Dussan
authors.initials: D
authors.lastname: Logar
authors.forename: Matija
authors.initials: M
authors.lastname: Tomsic
authors.forename: Blaz
authors.initials: B
authors.lastname: Rozman
authors.forename: Vita
authors.initials: V
authors.lastname: Dolzan
mesh_terms: D019943:Amino Acid Substitution; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D001483:Base Sequence; D002374:Catalase / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D018598:Minisatellite Repeats; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D013482:Superoxide Dismutase / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D017931:DNA Primers; D005819:Genetic Markers; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D014409:Tumor Necrosis Factor-alpha; D000099156:Superoxide Dismutase 2; C496319:NOS2 protein, human; C441642:SOD3 protein, human