title: Parameters of healing in approximative intestinal anastomosis.
abstract: BACKGROUND
  The restoration of bowel continuity using multiple classic anastomoses is mostly impossible in unstable critically ill extremely low birth weight neonates. The parameters of healing of approximative anastomoses in which integrity and continuity of bowel is achieved with limited number of stitches were evaluated in an experimental study.
  
  
  METHODS
  Small bowel anastomoses were performed in twenty-two adult male rats. An approximative ileo-ileal anastomosis was performed with five seromuscular-interrupted sutures only; in the control group the anastomosis was performed with the conventional technique of interrupted sutures. The mechanical and biochemical parameters were compared.
  
  
  RESULTS
  All anastomoses in both groups healed well without obstruction. The mean operating time needed for an approximative anastomosis was shorter (16 +/- 7.1 min versus 23.6 +/- 6.2 min, p = 0.016). The strength of the approximative anastomoses on the 1st day after surgery was 55 +/- 15 torr; the strength of the conventional anastomoses was 55 +/- 42 torr. The strength of the approximative anastomoses after 7 days was 249 +/- 39 torr; the strength of the conventional anastomoses was 218 +/- 23 torr (p = 0.118). The activity of the collagenolytic enzymes matrix metalloproteinase-2 and matrix metalloproteinase-9 in the anastomotic area was significantly increased compared with the activity in samples of non-operated bowel. There was no significant difference in collagenolytic activity between both types of anastomoses.
  
  
  CONCLUSIONS
  The approximative anastomosis is a time-saving alternative to conventional anastomoses with a comparable course of anastomotic healing, anastomotic strength, and changes in collagen metabolism.
authors.affiliation: Department of Pediatric Surgery, Charles University in Prague - 2nd Medical School, Prague, Czech Republic. mrygl@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Rygl
authors.forename: J
authors.initials: J
authors.lastname: Novotna
authors.forename: J
authors.initials: J
authors.lastname: Herget
authors.forename: R
authors.initials: R
authors.lastname: Skaba
authors.forename: J
authors.initials: J
authors.lastname: Snajdauf
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D013505:Digestive System Surgical Procedures / Q000379:methods; D004195:Disease Models, Animal; D007082:Ileum / Q000378:metabolism* / Q000601:surgery*; D007421:Intestine, Small / Q000601:surgery; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014945:Wound Healing
chemical_list: D015415:Biomarkers; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9