Apoptotic-related protein changes induced by hexavalent chromium in mice liver.
Although it has been reported that hexavalent chromium (Cr(VI)) could induce apoptosis in a variety of cell types, the molecular mechanisms underlying this process is still largely unknown. This study was undertaken to determine effects of single oral 0, 25, 50, and 100 mg/kg body weight doses of potassium dichromate on the expression level of p53, Bcl-2, Bax, cytochrome c, and caspase-3, which are vital regulators of apoptosis, in mice liver. The results showed that Cr(VI) could upregulate the protein expression of p53, Bax, cytochrome c, and caspase-3 and downregulate the expression of Bcl-2 in mice liver. All these results suggested that p53, Bcl-2, Bax, cytochrome c, and caspase-3 may be involved in the regulation of Cr(VI) induced apoptosis in vivo.
Wang
Xiao-Feng
XF
Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, China.
Lou
Xiao-Ming
XM
Shen
Ying
Y
Xing
Ming-Luan
ML
Xu
Li-Hong
LH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D011192:Potassium Dichromate / Q000633:toxicity*
D051017:Apoptosis Regulatory Proteins; D011192:Potassium Dichromate