Shinella yambaruensis sp. nov., a 3-methyl-sulfolane-assimilating bacterium isolated from soil.
A bacterial strain, designated MS4(T), was isolated from soil in the Ryukyu Archipelago, Japan. The bacterium grew with 3-methyl sulfolane as sole sulfur source. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain MS4(T) belonged to the genus Shinella; it was closely related to the type strains of Shinella granuli and Shinella zoogloeoides (16S rRNA gene sequence similarities of 98.2 and 96.7 %, respectively). Strain MS4(T) was a Gram-negative, non-motile, rod-shaped, aerobic bacterium. The major respiratory quinone was ubiquinone-10 and the predominant cellular fatty acid was C(18 : 1)omega7c. The DNA G+C content was 66.4 mol%. Based on phylogenetic and phenotypic traits, it was concluded that the organism represents a novel species in the genus Shinella for which the name Shinella yambaruensis sp. nov. is proposed; the type strain is MS4(T) (=NBRC 102122(T)=DSM 18801(T)).
Matsui
Toru
T
Center of Molecular Biosciences, University of the Ryukyus, Nishihara-cho, Okinawa 903-0213, Japan. tmatsui@comb.u-ryukyu.ac.jp
Shinzato
Naoya
N
Tamaki
Hideyuki
H
Muramatsu
Mizuho
M
Hanada
Satoshi
S
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008698:Mesylates / Q000378:metabolism*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008698:Mesylates; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; C045880:methanesulfonic acid