Effects of yessotoxin (YTX) on the skeletal muscle: an update.
Yessotoxins (YTXs) are algal toxins originally included in the diarrheic toxins. After oral intake, YTXs induce only ultra-structural changes (packages of swollen mitochondria) in cardiac cells. The aim of this study was to investigate the possible effects of YTX on the other contractile striated tissue, the skeletal muscle, in vitro and in vivo. In vitro, in skeletal mouse myotubes, YTX (0.01-1.0 microM) influenced cell excitability in a concentration- and time-dependent way. In the in vivo study, transmission electron microscopy analysis did not reveal any ultrastructural alteration of skeletal muscle after acute (1 mg kg(-1)) or repeated (1 and 2mg kg(-1) day(-1), for 7 days) oral administration of YTX to mice. The observation that effects were detected in vitro but not in vivo supports the hypothesis of a low YTX bioavailability to skeletal muscle after oral intake. Therefore, the results seem to exclude a toxic effect in skeletal muscle when YTX is consumed as a food contaminant.
Tubaro
A
A
Department of Materials and Natural Resources, University of Trieste, Trieste, Italy. tubaro@units.it
Bandi
E
E
Sosa
S
S
Soranzo
M R
MR
Giangaspero
A
A
De Ninis
V
V
Yasumoto
T
T
Lorenzon
P
P
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D018715:Microscopy, Video / Q000379:methods; D008978:Mollusk Venoms / Q000494:pharmacology; D018485:Muscle Fibers, Skeletal / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000648:ultrastructure; D035964:Oxocins / Q000494:pharmacology*; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects
D008978:Mollusk Venoms; D035964:Oxocins; C066632:yessotoxin