title: Expression of amyloid precursor protein after rat traumatic brain injury.
abstract: OBJECTIVE
  Previous reports have demonstrated that some focal brain injuries increase amyloid precursor protein (APP) immunoreactivity in the region surrounding the injury in the cerebral cortex. However, the chronologic changes in APP expression have not been evaluated after traumatic brain injury (TBI).
  
  
  METHODS
  In this study, we immunohistochemically and biologically investigated chronologic changes in cellular sources and levels of APP production after rat TBI.
  
  
  RESULTS
  In the present report, we show that traumatic brain injury increased the expression of APP in the neuronal perikarya and in damaged dystrophic neurites from 1 to 90 days after injury. Moreover, 7 days after injury, some macrophages/microglia also were co-localized with APP, which was overproduced by the neuronal perikarya and APP-positive dystrophic neurites after injury and then APP were phagocytosed by macrophages/microglia during this phase. However, astroglia did not express APP immunopositivity after brain injury.
  
  
  CONCLUSIONS
  These results suggested that long-term overexpression of APP was confirmed by immunohistochemical and biologic technique after TBI. This may be related to the induction of Alzheimer type dementia and it is a very important risk factor for this disease.
authors.affiliation: Department of Pathology, Kinki University School of Medicine, Okasayyama City, Osaka, Japan. tatsuki@med.kindai.ac.jp
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Itoh
authors.forename: Takao
authors.initials: T
authors.lastname: Satou
authors.forename: Shozo
authors.initials: S
authors.lastname: Nishida
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsubaki
authors.forename: Shigeo
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger