title: When starting over makes more sense.
abstract: Proofreading mechanisms intrinsic to DNA and RNA polymers that contribute substantially to overall fidelity are lacking in the ribosome. New evidence, however, suggests that quality control in translation can occur after substrate incorporation by an abortive mechanism entailing premature release factor-catalyzed termination. These data shed new light on the importance and ubiquity of retrospective quality control mechanisms in ensuring the overall fidelity of nature's processive enzymes and demonstrate that competitive elongation reactions on the ribosome are kinetically sensitive to compositional features of the translating particle.
authors.affiliation: Department of Physiology and Biophysics, Weill Cornell Medical College of Cornell University, New York, New York 10021, USA. scb2005@med.cornell.edu
authors.forename: Scott C
authors.initials: SC
authors.lastname: Blanchard
mesh_terms: D049148:Aminoacylation; D020137:Base Pair Mismatch / Q000235:genetics; D055162:Biocatalysis; D008954:Models, Biological; D008958:Models, Molecular; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D010454:Peptide Termination Factors; D012343:RNA, Transfer