Validation of six closely linked STRs located in the chromosome X centromere region.
We propose that clusters of closely linked markers, which segregate as stable haplotypes, provide a high potential to solve complex kinship cases. It is known that the X-chromosomal centromere region shows an extremely low degree of recombination. Hence, we focused our interest on the region between 56 and 64 Mb distant from the Xp telomere and considered 6 STRs which are now registered in the Genome Data Base as DXS10161, DXS10159, DXS10162, DXS10163, DXS10164, and DXS10165. All of these markers show a tetranucleotide or pentanucleotide structure and exhibit high or medium polymorphic information content. As a peculiarity, DXS10163 is a combination of a pentanucleotide STR and an 18 bp INDEL polymorphism. We report here the primer sequences, the repeat structures, the allele distributions and parameters of forensic interest for a German population sample.
Edelmann
Jeanett
J
Institut für Rechtsmedizin, Universität Leipzig, Leipzig, Germany.
Hering
Sandra
S
Augustin
Christa
C
Kalis
Stefan
S
Szibor
Reinhard
R
D000483:Alleles; D002503:Centromere / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D005858:Germany; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D017931:DNA Primers