Treatment of experimental periodontal disease by photodynamic therapy in immunosuppressed rats.
OBJECTIVE
The aim of this study was to compare photodynamic therapy (PDT) as an adjunctive treatment of induced periodontitis with scaling and root planing (SRP) in dexamethasone-inhibited rats.


METHODS
The animals were divided into two groups: ND (n=90), saline solution treatment; D (n=90), dexamethasone treatment. In the ND and D Groups, periodontal disease was ligature-induced at the first mandibular molar. After 7 days, the ligature was removed and all animals received SRP and were divided according to the following treatments: SRP, saline solution; Toluidine Blue-O (TBO), phenothiazinium dye; and PDT, TBO and laser irradiation. Ten animals in each treatment were killed at 7, 15 and 30 days. The radiographic and histometric values were statistically analysed.


RESULTS
In the ND and D Groups, radiographic analysis showed less bone loss in animals treated by PDT in all the experimental periods than SRP and TBO at 15 days (p<0.05). After a histometric analysis was carried out in the ND and D groups, the animals treated by PDT showed less bone loss in all periods than SRP and TBO after 15 days (p<0.05).


CONCLUSIONS
The PDT was an effective adjunctive treatment of induced periodontitis compared with SRP in dexamethasone-inhibited rats.
Fernandes
Leandro Araújo
LA
GEPLO - Group of Study and Research on Laser in Dentistry, Division of Periodontics, Department of Surgery and Integrated Clinic, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
de Almeida
Juliano Milanezi
JM
Theodoro
Leticia Helena
LH
Bosco
Alvaro Francisco
AF
Nagata
Maria José Hitomi
MJ
Martins
Thiago Marchi
TM
Okamoto
Tetuo
T
Garcia
Valdir Gouveia
VG
D016301:Alveolar Bone Loss / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D000704:Analysis of Variance; D000818:Animals; D012534:Dental Scaling; D003907:Dexamethasone / Q000276:immunology; D004195:Disease Models, Animal; D007166:Immunosuppressive Agents / Q000276:immunology; D028022:Low-Level Light Therapy / Q000379:methods; D008297:Male; D008334:Mandible / Q000473:pathology; D010518:Periodontitis / Q000150:complications / Q000276:immunology / Q000628:therapy*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014048:Tolonium Chloride / Q000627:therapeutic use*; D016896:Treatment Outcome
D007166:Immunosuppressive Agents; D017319:Photosensitizing Agents; D014048:Tolonium Chloride; D003907:Dexamethasone