Optimized method for the determination of itopride in human plasma by high-performance liquid chromatography with fluorimetric detection.
A high-performance liquid chromatographic method with fluorescence detection for the determination of itopride in human plasma is reported. The sample preparation was based on liquid-liquid extraction of itopride from plasma with t-butylmethylether and dichloromethane (70:30, v/v) mixture followed by a back extraction of the analyte to the phosphate buffer (pH 3.2). Liquid chromatography was performed on an octadecylsilica column (55 mm x 4 mm, 3 microm particles), the mobile phase consisted of acetonitrile-triethylamine-15 mM dihydrogenpotassium phosphate (14.5:0.5:85, v/v/v), pH of the mobile phase was adjusted to 4.8. The run time was 3 min. The fluorimetric detector was operated at 250/342 nm (excitation/emission wavelength). Naratriptan was used as the internal standard. The limit of quantitation was 9.5 ng/ml using 0.5 ml of plasma. The method precision and inaccuracy were less than 8%. The assay was applied to the analysis of samples from a bioequivalence study.
Ptácek
Pavel
P
Pharmakl s.r.o., Seydlerova 2451, CZ-15800 Prague 13, Czech Republic.
Klíma
Josef
J
Macek
Jan
J
D000293:Adolescent; D000328:Adult; D001549:Benzamides / Q000097:blood*; D001593:Benzyl Compounds / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D001549:Benzamides; D001593:Benzyl Compounds; C102254:itopride