title: Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology.
abstract: Optimization of media components for the growth and biomass production of Halobacterium salinarum VKMM 013 was carried out using response surface methodology. A second order quadratic model was estimated and media components were determined based on quadratic regression equation generated by model. These were 6.35 g L(-1) of KCl, 9.70 g L(-1) of MgSO(4), 13.38 g L(-1) of gelatin and 12.00 g L(-1) of soluble starch in nutrient broth supplemented with artificial seawater with 20% (w/v) of NaCl. In these optimal conditions, the obtained cell concentration of 0.746 g L(-1) dry weight was in agreement with the predicted cell concentration. The optimized media significantly shortened the time required for cell culture to reach the stationary phase while providing a nearly 2.4-fold increase in biomass production. Furthermore, in cell cultures of three other halophilic archaea the use of optimized media enhanced growth rate and provided high-cell density.
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, India. bhagatmani@yahoo.co.in
authors.forename: Muthu
authors.initials: M
authors.lastname: Manikandan
authors.forename: Lejla
authors.initials: L
authors.lastname: Pasić
authors.forename: Vijayaraghavan
authors.initials: V
authors.lastname: Kannan
mesh_terms: D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D006216:Halobacteriaceae / Q000254:growth & development*; D008954:Models, Biological; D011786:Quality Control
chemical_list: D003470:Culture Media