Increasing CoQ10 production by Rhodopseudomonas palustris J001 using a two-stage fermentation process.
CoQ10 is used not only as a medicine but also as a food supplement due to its various physiological activities. The production of CoQ10 by microbes is a successful approach for generating large amounts of this natural product. The effects of dissolved oxygen (DO) contents and the two-stage fermentation process on cell growth and CoQ10 production by Rhodopseudomonas palustris J001 were investigated. The optimal DO contents for cell growth and CoQ10 production were 45% and 15%, respectively. A two-stage fermentation process, which consists of a 1st stage with 45% DO, a 2nd stage with 15% DO and a synchronous feeding of 2.0% NaAc at the switching time (42 h after inoculation), has proven to be the optimum fermentation process for the production of CoQ10. The maximum biomass, CoQ10 production and CoQ10 production rate were 1.31 g l(-1), 89.1 mg l(-1), and 1.142 mg l(-1) h(-1), respectively, increased by 28%, 585% and 426% as compared to the one-stage batch production with 45% DO. The DO level was the major factor to increase the CoQ10 production by the two-stage process.
Jiang
Shi-yun
SY
Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. jiangshiyun@126.com
Yu
Long-jiang
LJ
D002455:Cell Division; D005285:Fermentation; D007700:Kinetics; D010100:Oxygen / Q000494:pharmacology; D012241:Rhodopseudomonas / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D014451:Ubiquinone; C024989:coenzyme Q10; D010100:Oxygen