title: Derivatization of 11-alpha-hydroxyprogesterone using phosphoramidite chemistry.
abstract: The well-known phosphoramidite chemistry routinely used in solid-phase oligonucleotide synthesis is exploited here in the preparation of steroid conjugates in solution. The applicability of the method is tested by conjugating one nucleosidic and one non-nucleosidic phosphoramidite building block to 11-alpha-hydroxyprogesterone. The suitability of one of the conjugates synthesized (5) for a competitive binding assay is also demonstrated.
authors.affiliation: Genetic Screening, PerkinElmer, Turku, Finland.
authors.forename: Janne
authors.initials: J
authors.lastname: Ketola
authors.forename: Pertti
authors.initials: P
authors.lastname: Hurskainen
authors.forename: Jari
authors.initials: J
authors.lastname: Hovinen
mesh_terms: D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006908:Hydroxyprogesterones / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D006908:Hydroxyprogesterones; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C030228:11-hydroxyprogesterone