3-Penten-2-one, a novel aldehyde adduct, is a biomarker for increased acetaldehyde in urine.
Diagnostic profiling of urine for volatile compounds of around 400 patients using headspace solid-phase microextraction (HS-SPME) in alkaline conditions identified 3-penten-2-one (approximately 1 to >6.3 micromol/L) in 26 patients. Five were in barbiturate coma. 3-Penten-2-one, previously of unknown origin, was shown to be formed by aldol condensation of acetaldehyde with acetone or acetoacetate during analysis. Semi-quantification of acetaldehyde using in-fibre derivatisation HS-SPME, showed high concentrations in five urine (33-348 micromol/L) and two plasma (17 and 43 micromol/L) samples. Hence, urinary 3-penten-2-one is a useful biomarker for increased accumulation of acetaldehyde during abnormal metabolic stress.
Walker
Valerie
V
Clinical Biochemistry, Level D, South Block, Southampton General Hospital, Tremona Road, Southampton, Hampshire SO16 6YD, UK.
Mills
Graham A
GA
Stansbridge
Elizabeth M
EM
D000079:Acetaldehyde / Q000097:blood / Q000737:chemistry / Q000652:urine; D000293:Adolescent; D000328:Adult; D000447:Aldehydes / Q000097:blood / Q000737:chemistry* / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000737:chemistry / Q000652:urine; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D000447:Aldehydes; D015415:Biomarkers; D000079:Acetaldehyde