title: Genetic consequences of glacial survival and postglacial colonization in Norway spruce: combined analysis of mitochondrial DNA and fossil pollen.
abstract: Norway spruce (Picea abies [L.] Karst.) is a broadly distributed European conifer tree whose history has been intensively studied by means of fossil records to infer the location of full-glacial refugia and the main routes of postglacial colonization. Here we use recently compiled fossil pollen data as a template to examine how past demographic events have influenced the species' modern genetic diversity. Variation was assessed in the mitochondrial nad1 gene containing two minisatellite regions. Among the 369 populations (4876 trees) assayed, 28 mitochondrial variants were identified. The patterns of population subdivision superimposed on interpolated fossil pollen distributions indicate that survival in separate refugia and postglacial colonization has led to significant structuring of genetic variation in the southern range of the species. The populations in the northern range, on the other hand, showed a shallow genetic structure consistent with the fossil pollen data, suggesting that the vast northern range was colonized from a single refugium. Although the genetic diversity decreased away from the putative refugia, there were large differences between different colonization routes. In the Alps, the diversity decreased over short distances, probably as a result of population bottlenecks caused by the presence of competing tree species. In northern Europe, the diversity was maintained across large areas, corroborating fossil pollen data in suggesting that colonization took place at high population densities. The genetic diversity increased north of the Carpathians, probably as a result of admixture of expanding populations from two separate refugia.
authors.affiliation: Norwegian Forest and Landscape Institute, Høgskoleveien 12, NO-1432 As, Norway.
authors.forename: Mari Mette
authors.initials: MM
authors.lastname: Tollefsrud
authors.forename: Roy
authors.initials: R
authors.lastname: Kissling
authors.forename: Felix
authors.initials: F
authors.lastname: Gugerli
authors.forename: Øystein
authors.initials: Ø
authors.lastname: Johnsen
authors.forename: Tore
authors.initials: T
authors.lastname: Skrøppa
authors.forename: Rachid
authors.initials: R
authors.lastname: Cheddadi
authors.forename: W O
authors.initials: WO
authors.lastname: Van der Knaap
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Latałowa
authors.forename: Ruth
authors.initials: R
authors.lastname: Terhürne-Berson
authors.forename: Thomas
authors.initials: T
authors.lastname: Litt
authors.forename: Thomas
authors.initials: T
authors.lastname: Geburek
authors.forename: Christian
authors.initials: C
authors.lastname: Brochmann
authors.forename: Christoph
authors.initials: C
authors.lastname: Sperisen
mesh_terms: D004272:DNA, Mitochondrial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005060:Europe; D019143:Evolution, Molecular; D005580:Fossils; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D018598:Minisatellite Repeats; D010802:Phylogeny; D028222:Picea / Q000235:genetics*; D011058:Pollen / Q000235:genetics*; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA; D014197:Trees / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D018744:DNA, Plant