title: In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method.
abstract: The antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from Goiania city patients, Brazil. In this study, the microtiter plates were incubated at 28 masculineC allowing a reading of the minimal inhibitory concentration (MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03 microg/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole, ketoconazole and terbinafine, respectively.
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás, Goiânia, GO, Brasil. rosario@iptsp.ufg.br
authors.forename: Crystiane Rodrigues
authors.initials: CR
authors.lastname: Araújo
authors.forename: Karla Carvalho
authors.initials: KC
authors.lastname: Miranda
authors.forename: Orionalda de Fatima Lisboa
authors.initials: Ode F
authors.lastname: Fernandes
authors.forename: Ailton José
authors.initials: AJ
authors.lastname: Soares
authors.forename: Maria do Rosário Rodrigues
authors.initials: Mdo R
authors.lastname: Silva
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D008864:Microsporum / Q000145:classification / Q000187:drug effects*; D014249:Trichophyton / Q000145:classification / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole; D015725:Fluconazole; D007654:Ketoconazole