[Relationship between protein oxidation levels in the follicular fluid and the outcome parameters of in vitro fertilization-embryo transplantation].
OBJECTIVE
To explore the relationship between protein oxidation levels in the follicular fluid and the outcome parameters of in vitro fertilization-embryo transplantation (IVF-ET).


METHODS
The levels of advanced oxidation protein products (AOPP) in the follicular fluid were measured in 64 women with tubal infertility undergoing IVF-ET. The relationship between the AOPP levels and IVF-ET outcome parameters was analyzed.


RESULTS
AOPP levels showed significant inverse correlations between the proportion of mature oocytes (r=-0.401, P=0.001), fertilization rate (r=-0.257, P=0.045), cleavage rate (r=-0.290, P=0.024) and good embryo rate (r=-0.520, P=0.000). AOPP levels differed significantly between the groups with different retrieved oocyte numbers (F=3.851, P=0.027), being the lowest in women with 8 to 15 retrieved oocytes and the highest in those with retrieved oocytes below 8. The AOPP level in the non-pregnant women was significantly higher than that in the pregnant women (t=3.665, P=0.001). The AOPP levels also differed significantly with age (F=15.919, P=0.000), and the women >35 years of age had the highest level and those below 30 years had the lowest level.


CONCLUSIONS
Protein oxidative stress is present in the follicular fluid of women on IVF-ET cycles. High level of AOPP may have adverse effects on the oocytes and early embryonic development and may affect the outcome of IVF-ET.
Song
Ya-li
YL
Reproductive Technology Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. syl950446@126.com
Quan
Song
S
Tian
Jian-wei
JW
Li
Hong
H
Chen
Si-mei
SM
Xing
Fu-qi
FQ
D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism / Q000628:therapy; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011247:Pregnancy; D018873:Pregnancy Rate; D011506:Proteins / Q000378:metabolism*
D011506:Proteins