Enantioseparation of amino acid amides on an (S)-N-(3,5-dinitrobenzoyl)leucine-N-phenyl-N-propylamide-bonded silica in normal phase CEC.
(S)-N-(3,5-Dinitrobenzoyl)leucine-N-phenyl-N-propylamide-bonded silica was used as a chiral stationary phase for separation of enantiomers of some racemic pi-acidic and pi-basic amino acid amides in normal phase capillary LC (CLC) and CEC. For generation of EOF in CEC, different amounts of water were added to n-hexane-isopropanol (IPA) eluents. Influences of added water and composition of polar modifier on retention, enantioselectivity and resolution were studied. Generally better separation was obtained in the eluents of lower water content. Shorter retention and better enantioselectivities were observed in the eluents of higher IPA content, whereas resolution became lower as IPA content was increased. Enantioselectivities were comparable but resolutions were much better in CEC than CLC.
Kim
In Whan
IW
Department of Chemistry Education, Daegu University, Gyeongsan, Korea.
Hyun
Myung Ho
MH
Gwon
Jurim
J
Jin
Juhyeon
J
Park
Jung Hag
JH
D019840:2-Propanol / Q000737:chemistry; D000577:Amides / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D053801:Capillary Electrochromatography; D004136:Dinitrobenzenes / Q000737:chemistry; D053841:Electroosmosis; D007930:Leucine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
D000577:Amides; D000596:Amino Acids; D004136:Dinitrobenzenes; D014867:Water; D012822:Silicon Dioxide; D007930:Leucine; D019840:2-Propanol