Correlation of CD34+ cells with tissue angiogenesis after traumatic brain injury in a rat model.
Increasing evidence suggests that circulating endothelial progenitor cells, which are a subpopulation of hematopoietic progenitor CD34(+) cells, play a critical role in neovascularization and tissue repair. We have tested the hypothesis that traumatic brain injury (TBI) could mobilize CD34(+) cells to peripheral blood and brain tissue, a process critical for vascular repair, in a rat model of TBI. Male Wistar rats were subjected to controlled fluid percussion. Blood and brain tissue were collected before and after TBI to measure the levels of CD34(+) cells in peripheral blood and to detect their accumulation in the damaged cerebral tissue. Compared with surgery controls, CD34(+) cells significantly increased in the peripheral blood and accumulated in the brain tissue of TBI rats. Immunohistochemistry detected new vessels with incomplete CD34(+) endothelial-like cell lining and an increased number of microvessels in the injured and surrounding tissue. The results demonstrate a close correlation between an increase in circulating CD34(+) cells in response to traumatic injury and angiogenesis in TBI rat brain. They also suggest that transplantation of CD34(+) cells or augmentation of endogenous CD34(+) cells may be a novel therapeutic approach for patients with TBI.
Guo
Xinbin
X
Department of Neurosurgery, Tianjin Medical University, Laboratory of TJIVR, Tianjin Neurological Institute, Tianjin, China.
Liu
Li
L
Zhang
Ming
M
Bergeron
Angela
A
Cui
Zhuang
Z
Dong
Jing-Fei
JF
Zhang
Jianning
J
D000704:Analysis of Variance; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D002454:Cell Differentiation; D049109:Cell Proliferation; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D018952:Antigens, CD34