title: The porcine ANG, RNASE1 and RNASE6 genes: molecular cloning, polymorphism detection and the association with haematological parameters.
abstract: Angiogenin (ANG) [also known as ribonuclease, RNase A family, 5 (RNASE5)], ribonuclease, RNase A family, 1 (pancreatic) (RNASE1) and ribonuclease, RNase A family, k6 (RNASE6) are three members of the RNase A superfamily. It has been suggested that these three genes play important roles in host defense. In this study, we obtained the whole open reading frame (ORF) of each gene and found the deduced proteins contain some similar structures harboring a catalytic triad and an invariant "CKXXNTF" signature motif. One single nucleotide polymorphism (SNP) was detected in each gene (g. 149G>T polymorphism in the porcine ANG gene, which resulted in an amino acid change from glycine to valine, g. 296A>G polymorphism in the porcine RNASE1 gene and g. 389C>T polymorphism in the porcine RNASE6 gene). Association analyses revealed the significant associations (P < 0.05) between the porcine ANG g. 149G>T polymorphism and mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean platelet volume (MPV) and platelet-large cell ratio (P-LCR) measured on 0-day-old pigs and MCV measured at 32 days after birth. The porcine RNASE6 g. 389C>T polymorphism was significantly associated (P < 0.05) with MCV, MCH and neutrophil percentage (NEI %) measured on 0-day-old pigs, respectively. Our current findings, if confirmed by other studies, might shed some light on the roles of the investigated genes in host defense.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong (Central China) Agricultural University, 430070 Wuhan, Hubei, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Zian
authors.initials: Z
authors.lastname: Liang
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Liu
authors.forename: Mengjin
authors.initials: M
authors.lastname: Zhu
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Wu
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001790:Blood Physiological Phenomena / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004722:Endoribonucleases / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012259:Ribonuclease, Pancreatic / Q000235:genetics*; D013552:Swine / Q000235:genetics*
chemical_list: D004722:Endoribonucleases; C046366:angiogenin; C101685:ribonuclease k6; D012259:Ribonuclease, Pancreatic