Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid.
Tuberonic acid (12-hydroxy epi-jasmonic acid, TA) and its glucoside (TAG) were isolated from potato leaflets (Solanumtuberosum L.) and shown to have tuber-inducing properties. The metabolism of jasmonic acid (JA) to TAG in plant leaflets, and translocation of the resulting TAG to the distal parts, was demonstrated in a previous study. It is thought that TAG generated from JA transmits a signal from the damaged parts to the undamaged parts by this mechanism. In this report, the metabolism of TA in higher plants was demonstrated using [12-(3)H]TA, and a glucosyltransferase active toward TA was purified from the rice cell cultures. The purified protein was shown to be a putative salicylic acid (SA) glucosyltransferase (OsSGT) by MALDI-TOF-MS analysis. Recombinant OsSGT obtained by overexpression in Escherichia coli was active not only toward TA but also toward SA. The OsSGT characterized in this research was not specific, but this is the first report of a glucosyltransferase active toward TA. mRNA expressional analysis of OsSGT and quantification of TA, TAG, SA and SAG after mechanical wounding indicated that OsSGT is involved in the wounding response. These results demonstrated a crucial role for TAG not only in potato tuber formation, but also in the stress response in plants and that the SA glucosyltransferase can work for TA glucosylation.
Seto
Yoshiya
Y
Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku Sapporo 060-8589, Japan.
Hamada
Shigeki
S
Matsuura
Hideyuki
H
Matsushige
Mana
M
Satou
Chizuru
C
Takahashi
Kosaku
K
Masuta
Chikara
C
Ito
Hiroyuki
H
Matsui
Hirokazu
H
Nabeta
Kensuke
K
D000085:Acetates / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism*; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism*; D015394:Molecular Structure; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012459:Salicylates / Q000378:metabolism; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D005960:Glucosides; D010940:Plant Proteins; D011994:Recombinant Proteins; D012459:Salicylates; C421181:tuberonic acid glucoside; C421182:tuberonic acid; D005964:Glucosyltransferases