title: Application of 16S rRNA, cytochrome b and control region sequences for understanding the phylogenetic relationships in Oryx species.
abstract: The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective phylogenetic trees were compared. All these markers clearly differentiated the genus Addax from Oryx. However, for species-level grouping, while 16S rRNA and cytochrome b produced similar phylogeny (SHO grouped with PO), the control region grouped SHO with AO. Further studies are warranted to generate more sequencing data, apply multiple bioinformatics tools and to include relevant nuclear markers for phylogenetic analysis of Oryx species.
authors.affiliation: Molecular Fingerprinting and Biodiversity Unit, Prince Sultan Research Chair Program for Environment and Wildlife, College of Science, King Saud University, Riyadh, Saudi Arabia. khan_haseeb@yahoo.com
authors.forename: H A
authors.initials: HA
authors.lastname: Khan
authors.forename: I A
authors.initials: IA
authors.lastname: Arif
authors.forename: A A
authors.initials: AA
authors.lastname: Al Homaidan
authors.forename: A H
authors.initials: AH
authors.lastname: Al Farhan
mesh_terms: D000818:Animals; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D030561:Databases, Nucleic Acid; D019143:Evolution, Molecular; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012418:Ruminants / Q000145:classification / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D045303:Cytochromes b