Development and application of high-performance affinity beads: toward chemical biology and drug discovery.
In drug development research, the elucidation and understanding of the interactions between physiologically active substances and proteins that numerous genes produce is important. Currently, most commercially available drugs and physiologically active substances have been brought to market without knowledge of factors interacting with the drugs and the substances. Affinity purification is a useful and powerful technique employed to understand factors that are targeted by drugs and physiologically active substances. However, use of conventional matrices for affinity chromatography often causes a decrease in efficiency of affinity purification and, as a result, more practical matrices for affinity purification have been developed for application in drug discovery research. In this paper, we describe the development of high-performance affinity beads (SG beads and FG beads) that enable one-step affinity purification of drug targets and the elucidation of the mechanism of the action of the drugs. We also describe a chemical screening system using our affinity beads. We hope that utilization of the affinity beads will contribute to the progress of research in chemical biology.
Sakamoto
Satoshi
S
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501, Japan.
Kabe
Yasuaki
Y
Hatakeyama
Mamoru
M
Yamaguchi
Yuki
Y
Handa
Hiroshi
H
D001667:Binding, Competitive; D002846:Chromatography, Affinity / Q000379:methods*; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D052203:Ferrosoferric Oxide / Q000737:chemistry; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011485:Protein Binding
D011109:Polymethacrylic Acids; C524317:poly(styrene-co-glycidyl methacrylate); D052203:Ferrosoferric Oxide