Overexpression of a new gene P28GANK confers multidrug resistance of gastric cancer cells.
Here we investigated the roles of P28GANK in multidrug resistance of gastric cancer cells and the possible mechanisms. We constructed the siRNA vector of P28GANK and transfected it into human vincristine-resistant gastric adenocarcinoma cell line SGC7901/VCR. Down-regulation of P28GANK could enhance the sensitivity of SGC7901/VCR cells towards anticancer drugs and could decrease the capacity of cells to efflux adriamycin. P28GANK could down-regulate the expression of P-gp, but not affect MRP or GST. In vivo experiment also confirmed our above results. Further study of the biological functions of P28GANK might be helpful for understanding the mechanisms of MDR in gastric cancer.
Li
Xiaohua
X
State Key Laboratory of Cancer Biology, Xijing Hospital, the Fourth Military Medical University, Xi'an, China.
Zhang
Ying
Y
Xiong
Chen
C
Jin
Haifeng
H
Jing
Bin
B
Zhang
Yafei
Y
Fan
Daiming
D
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000493:pharmacokinetics; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics; D014183:Transplantation, Heterologous; D014700:Verapamil / Q000494:pharmacology
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C403591:PSMD10 protein, human; D011518:Proto-Oncogene Proteins; D004317:Doxorubicin; D014700:Verapamil; D005982:Glutathione Transferase; D046988:Proteasome Endopeptidase Complex