Stackebrandtia albiflava sp. nov. and emended description of the genus Stackebrandtia.
A novel actinomycete, designated YIM 45751(T), was isolated from a soil sample collected from the Xishuang Banna tropical rainforest, Yunnan province, south-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained ribose, xylose and glucose. The predominant menaquinones were MK-10(H(4)), MK-10(H(6)), MK-11(H(4)) and MK-11(H(6)). The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. 16S rRNA gene sequence analysis showed that Stackebrandtia nassauensis DSM 44728(T) was the closest phylogenetic neighbour (95.8 % 16S rRNA gene sequence similarity). On the basis of phylogenetic and some common chemotaxonomic data, the isolate should belong to the genus Stackebrandtia. However, phylogenetic analysis and comparison of physiological and chemotaxonomic characteristics demonstrated that the isolate is distinct from S. nassauensis DSM 44728(T). Strain YIM 45751(T) represents a separate species of the genus Stackebrandtia, for which the name Stackebrandtia albiflava sp. nov. is proposed. The type strain is YIM 45751(T) (=DSM 45044(T)=CCTCC AA 206003(T)). Furthermore, the description of the genus Stackebrandtia is also required to be emended.
Wang
Yong-Xia
YX
Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Zhi
Xiao-Yang
XY
Zhang
Yu-Qin
YQ
Cui
Xiao-Long
XL
Xu
Li-Hua
LH
Li
Wen-Jun
WJ
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014197:Trees; D014329:Tropical Climate
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S