Quelling targets the rDNA locus and functions in rDNA copy number control.
BACKGROUND
RNA silencing occurs in a broad range of organisms. Although its ancestral function is probably related to the genome defense mechanism against repetitive selfish elements, it has been found that RNA silencing regulates different cellular processes such as gene expression and chromosomal segregation. In Neurospora crassa, a RNA silencing mechanism, called quelling, acts to repress the expression of transgenes and transposons, but until now no other cellular functions have been shown to be regulated by this mechanism.


RESULTS
Here, we detected by northern blotting endogenous short interfering RNA (siRNAs) from the repetitive ribosomal DNA locus (rDNA) that are loaded onto the argonaute protein QDE-2. Moreover, we found a bidirectional transcription that can generate double strand RNA (dsRNA) molecules. Interestingly, quelling mutants have a reduced rDNA gene copy number.


CONCLUSIONS
Our finding could suggest a new biological function for RNA silencing in the maintenance of the integrity and stability of the Neurospora rDNA locus.
Cecere
Germano
G
Dipartimento di Biotecnologie Cellulari ed Ematologia, Università La Sapienza, Rome, Italy. gc2352@columbia.edu
Cogoni
Carlo
C
D015152:Blotting, Northern; D004275:DNA, Ribosomal / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D018628:Gene Dosage; D009492:Neurospora crassa / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
D004275:DNA, Ribosomal; D005656:Fungal Proteins; C406184:QDE-2 protein, Neurospora crassa; D034741:RNA, Small Interfering