Enhancement of DNA cleavage activity of an unnatural ferrocene-amino acid conjugate.
The nucleolytic activity of an unnatural ferrocenyl amino acid can be significantly enhanced under reducing or Fenton conditions while exploiting different mechanisms of DNA cleavage. In addition, results from high resolution electrophoresis indicate that the ferrocenyl amino acid exhibits non-sequence specific cleavage of the DNA backbone.
Higgins
Pamela J
PJ
Department of Chemistry, Dickinson College, Carlisle, PA 17013, USA.
Gellett
Amanda M
AM
D000596:Amino Acids / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003497:Cyclic N-Oxides / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D053837:DNA Cleavage; D004229:Dithiothreitol / Q000737:chemistry; D004586:Electrophoresis; D005296:Ferrous Compounds / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry; D008353:Mannitol / Q000737:chemistry; D000075163:Metallocenes; D008956:Models, Chemical; D012341:RNA, Ribosomal, 5S / Q000737:chemistry; D017382:Reactive Oxygen Species
D000596:Amino Acids; D000975:Antioxidants; D001386:Azides; D003497:Cyclic N-Oxides; D005296:Ferrous Compounds; D000075163:Metallocenes; D012341:RNA, Ribosomal, 5S; D017382:Reactive Oxygen Species; D008353:Mannitol; D004247:DNA; D008239:Lysine; D004229:Dithiothreitol; C004998:ferrocene; C003959:TEMPO