[Evaluation of myelin basic protein levels with receiver operating characteristic curves for diagnosis of multiple sclerosis].
OBJECTIVE
To assess the value of cerebral spinal fluid (CSF) and serum myelin basic protein (MBP) levels in the diagnosis of multiple sclerosis (MS).


METHODS
Enzyme-linked immunosorbent assay was used to detect the CSF and serum levels of MBP in patients with MS (n=45), patients with Guillain-Barre syndrome (GBS) (n=36) and control subjects (control) (n=33). The sensitivity and specificity of MBP in CSF and serum in the diagnosis of MS were evaluated using the receiver-operating characteristic (ROC) curves.


RESULTS
The MBP levels in CSF and serum both increased significantly in MS group as compared with those in GBS (P<0.01) and control groups (P<0.01). The area under the curve (AUC) of the ROC curve of MBP in CSF was 0.853-/+0.037 for MS diagnosis, and with the optimal cut-off value of 0.87 pg/ml, CSF MBP showed a diagnostic sensitivity of 83.7% and specificity of 78.3%. The AUC of the ROC curve of serum MBP was 0.761-/+0.046, and the optimal cut-off value of 0.25 pg/ml resulted in a diagnostic sensitivity of 62.8% and specificity of 73.9%. No statistically significant difference was found between the two AUCs (P>0.05).


CONCLUSIONS
Evaluation of CSF and serum MBP levels allows accurate diagnosis of MS, and MBP level in the CSF has greater diagnostic sensitivity than serum MBP. The combination of both CSF and serum MBP levels may serve as a sensitive index for the diagnosis of MS.
Tian
Zuo-jun
ZJ
Department of Neurology, First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China. tianzuojun9@163.com
Zhao
Xue-xu
XX
Li
Zuo-han
ZH
Zhang
Fan
F
Cao
Fu-tian
FT
Li
Shao-ming
SM
Shao
Ming
M
Dong
Ya-xian
YX
D000293:Adolescent; D000328:Adult; D000368:Aged; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis*; D004676:Myelin Basic Protein / Q000097:blood* / Q000134:cerebrospinal fluid*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D055815:Young Adult
D004676:Myelin Basic Protein