Cells extract from fetal liver promotes the hematopoietic differentiation of human embryonic stem cells.
Here, we have now developed a new inducing system to promote the differentiation of human stem cells (hESCs) toward hematopoietic lineages by the treatment with cells extract of human fetal liver tissue (hFLT). The embryoid bodies (EBs) obtained from human H1 embryonic stem cells were exposed to buffer, hFLT cells extract, heated hFLT cell extract, and cell extract of human liver cells lines-LO2. Then, the feature of EBs in different groups was characterized by real-time RT-PCR and colony-forming assays. The results showed the treatment by hFLT cells extract could activate the hematopoietic genes expression and improve the capacity for hematopoietic progenitor development of hEBs. After that, we cocultured hFLT extract treated hEBs on the hFLSCs (human fetal liver stromal cells) feeder to differentiate them into hematopoietic cells. As a control, untreated hEBs were cocultured on hFLSCs feeder with cytokines. The feature of induced cells from hEBs was characterized by flow cytometry, Wright-Giemsa staining, and colony-forming assays. The results demonstrated that hFLT cells extract was capable of inducing hEBs into hematopoietic cells and combing it with hFLSCs feeder could largely promote hematopoietic differentiation of hESCs. This method may supply a new way to substitute the cytokines required in hematopoietic induction of hESCs.
Liu
Yu-Xiao
YX
Beijing Institution of Transfusion Medicine, People's Republic of China.
Ji
Lei
L
Yue
Wen
W
Yan
Zhi-Feng
ZF
Wang
Jing
J
Xi
Jia-Fei
JF
Zhang
Rui
R
Nan
Xue
X
Bai
Ci-Xian
CX
Chen
Lin
L
Wang
Yun-Fang
YF
Pei
Xue-Tao
XT
D018952:Antigens, CD34 / Q000378:metabolism; D018929:Cell Culture Techniques; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005333:Fetus / Q000737:chemistry / Q000166:cytology; D015870:Gene Expression / Q000187:drug effects; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000196:embryology; D008110:Liver Extracts / Q000737:chemistry / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
D018952:Antigens, CD34; D002457:Cell Extracts; D008110:Liver Extracts; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human