title: The study of the changes in the biochemical and mineral contents of bones of Catla catla due to lead intoxication.
abstract: In the present study, an attempt has been made to analyze the changes in the biochemical and mineral contents of lead-intoxicated bones of Catla catla at subchronic (15.5 ppm) exposure, and also to determine whether the effects of Pb intoxication can be reversed with the chelating agent meso 2, 3-dimercaptosuccinic acid (DMSA) on the bones of freshwater fingerlings Catla catla by using Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and atomic absorption spectrophotometer techniques. The FT-IR spectra of the lead-exposed bones show significant alteration in the biochemical constituents. The XRD analysis showed a decrease in crystallinity due to lead exposure. Further, the Ca, Mg, and P contents of the lead-exposed bones were less than those of the control group, and there was an increase in the mineral contents of the bones after DMSA treatment. In conclusion, the present study suggests that the subchronic lead exposure results in severe loss of bone minerals. The overall decrease in the FT-IR band intensity of Pb-exposed bones relative to the control indicates a decrease in the biochemical constituents like proteins and lipids. The increase in the band intensity after treatment with chelating agent DMSA indicates increased biochemical constituents, showing that the subchronic effects of lead can be reversed by DMSA. The amide I bands observed at 1654 cm(-1) in the present study suggest that the protein is dominated by alpha-helical structure.
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India. plrmpl@hotmail.com
authors.forename: P L R M
authors.initials: PL
authors.lastname: Palaniappan
authors.forename: N
authors.initials: N
authors.lastname: Krishnakumar
authors.forename: M
authors.initials: M
authors.lastname: Vadivelu
authors.forename: V
authors.initials: V
authors.lastname: Vijayasundaram
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects* / Q000502:physiology*; D003530:Cyprinidae / Q000502:physiology*; D007854:Lead / Q000633:toxicity*; D007855:Lead Poisoning / Q000503:physiopathology*; D004113:Succimer; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D007854:Lead; D004113:Succimer