Isolation of two new prenylflavonols from Epimedium brevicornum and their effects on cytokine production in vitro.
In an attempt to search for bioactive natural products, two new prenylflavonols, 2''-hydroxy-3''-en-anhydroicaritin (1) and 2''-hydroxy-beta-anhydroicaritin (2), were isolated from the Chinese medicinal herb Epimedium brevicornum. Their structures were determined by 1D and 2D NMR spectroscopic analysis. The effects of compounds 1 and 2 on cytokine production in vitro were investigated. Compound 1 could significantly downregulate tumor necrosis factor-alpha (TNF-alpha) production and increase interleukin-10 (IL-10) production. These results suggested that compound 1 may have anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated mouse macrophages. In addition, the biogenetic relationships among compounds 1 and 2 are discussed.
Luo
Guo-Jun
GJ
College of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, P. R. China.
Ci
Xin-Xin
XX
Ren
Rong
R
Wu
Zhao-Yuan
ZY
Li
Hong-Mei
HM
Li
Hai-Zhou
HZ
Li
Rong-Tao
RT
Deng
Xu-Ming
XM
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D015536:Down-Regulation; D034181:Epimedium / Q000737:chemistry*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054876:Prenylation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
C543986:2'-hydroxy-3'-en-anhydroicaritin; C543985:2'-hydroxy-beta-anhydroicaritin; D000893:Anti-Inflammatory Agents; D044948:Flavonols; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10