Resonance light scattering spectral method for the determination of serum albumin with the interaction of neutral red-sodium dodecyl sulfonate.
Based on the enhancement of resonance light scattering (RLS) of serum albumin interaction with neutral red (NR) and sodium dodecyl sulfonate (SDS), a novel sensitive assay of serum albumins has been developed. Experimental conditions such as mixing sequence of reagents, pH, NR and SDS concentrations have been optimized. Linear relationships between the enhanced RLS intensity and the protein concentration were observed for bovine serum albumin (BSA) within the range of 0.01-5.0 microg mL(-1) and human serum albumin (HAS) of 0.01-7.0 microg mL(-1). The detection limits (S/N=3) are 6.0 ng mL(-1) for BSA and 5.0 ng mL(-1) for HAS, respectively. The method was successfully applied to the determination of HSA in human blood plasma samples with recovery from 97.3 to 104.3%.
Zhan
Guoqing
G
Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
Zhang
Lixia
L
Li
Chunya
C
D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry*; D002417:Cattle; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009499:Neutral Red / Q000737:chemistry; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
D001557:Benzenesulfonates; D012709:Serum Albumin; D009499:Neutral Red; D012710:Serum Albumin, Bovine; C001114:dodecylbenzenesulfonic acid