title: Oct4 is expressed in Nestin-positive cells as a marker for pancreatic endocrine progenitor.
abstract: There are abundant progenitor cells in the developing pancreas, but molecular markers for these cells are lacking. Octamer-binding transcription factor-4 (Oct4) is an important transcription factor for keeping the features of self-renewal and pluripotency of embryonic stem cells. It's well known that Oct4, as a totipotent stem cells marker, just is expressed in totipotent stem cells. In the present study, we collected ten human fetal pancreases, and found that Oct4 mRNA and protein were expressed in human fetal pancreas samples by RT-PCR, western blot and immunohistochemistry assays. Using double-staining, we demonstrated that Oct4 was not co-expressed with Chromogranin A (a peptide expressed in endocrine cells), but partially co-expressed with Ngn3 (a transcription factor expressed in pancreatic endocrine precursor cells) and Nestin (a intermediate filament, Nestin-positive cells isolated from islets can be induced to express insulin) in human fetal pancreases. Indeed, we prepared Nestin-positive cells from human fetal pancreas by cell selection, and found that these cells expressed Oct4 and Ngn3. The Nestin-positive cells displayed a rapid duplication and could differentiate into osteoblasts, fat and endocrine cells in vitro. These results indicated that the Nestin-positive cells in the fetal age should be pancreatic progenitor cells. Overall, our study suggested that Oct4 was a marker for pancreatic endocrine progenitor.
authors.affiliation: Stem Cell Research Center, Health Science Center, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing, China. eebytht@gmail.com
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Shuling
authors.initials: S
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Kong
authors.forename: Shen
authors.initials: S
authors.lastname: Chen
authors.forename: Lingsong
authors.initials: L
authors.lastname: Li
authors.forename: Liying
authors.initials: L
authors.lastname: Li
mesh_terms: D037881:Aborted Fetus; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D053379:Chromogranin A / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D064231:Nestin; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D053379:Chromogranin A; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D050814:Octamer Transcription Factor-3; C577484:NES protein, human; C498695:NEUROG3 protein, human; C494173:POU5F1 protein, human