title: Nevirapine resistance in human immunodeficiency virus type 1-positive infants determined using dried blood spots stored for up to six years at room temperature.
abstract: Dried blood spots that had been stored ambiently for 3 to 6 years lost approximately 1 log(10) of human immunodeficiency virus type 1 (HIV-1) RNA, but the majority could still be genotyped for resistance. Nevirapine resistance was found in 7/16 (43.5%) HIV-1-positive HIVNET 024 infants at 4 to 6 weeks, but no resistance was found at other time points.
authors.affiliation: Department of Microbiology and Immunology, UNC Center for AIDS Research, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. jnelson@med.unc.edu
authors.forename: Julie A E
authors.initials: JA
authors.lastname: Nelson
authors.forename: Amy M
authors.initials: AM
authors.lastname: Loftis
authors.forename: Deborah
authors.initials: D
authors.lastname: Kamwendo
authors.forename: Wafaie W
authors.initials: WW
authors.lastname: Fawzi
authors.forename: Taha E
authors.initials: TE
authors.lastname: Taha
authors.forename: Robert L
authors.initials: RL
authors.lastname: Goldenberg
authors.forename: Susan A
authors.initials: SA
authors.lastname: Fiscus
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D001769:Blood / Q000821:virology*; D003890:Desiccation; D024882:Drug Resistance, Viral; D005838:Genotype; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019829:Nevirapine / Q000494:pharmacology*; D012367:RNA, Viral / Q000235:genetics*; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral; D019829:Nevirapine