Overview on concepts and applications of Fab antibody fragments.
In this overview, the Fab molecule is introduced and discussed as the first generated antibody fragment, which still dominates basic research and clinical applications. The unit contains a concepts section and an applications section. In the concepts section, the two principal methods for producing Fab, as well as the generation and directed evolution of Fab by phage display, are described. The applications section discusses Fab in clinical applications, as well as their increasingly important role in the determination of the three-dimensional structures of transmembrane proteins.
Rader
Christoph
C
Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
D003460:Crystallization; D010450:Endopeptidases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics* / Q000378:metabolism / Q000627:therapeutic use*; D008565:Membrane Proteins / Q000648:ultrastructure; D019151:Peptide Library; D011485:Protein Binding
D007140:Immunoglobulin Fab Fragments; D008565:Membrane Proteins; D019151:Peptide Library; D010450:Endopeptidases