title: Stages in ELISA.
abstract: This chapter gives general information on essential practical features of ELISAs. These can be summarized as follows: 1. Adsorption of antigen or antibody to the plastic solid phase. 2. Addition of the test sample and subsequent reagents. 3. Incubation of reactants. 4. Separation of bound and free reactants by washing. 5. Addition of enzyme-labeled reagent. 6. Addition of enzyme detection system (color development). 7. Visual or spectrophotometric reading of the assay.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans
chemical_list: D000906:Antibodies; D000941:Antigens