Purification and characteristics of feruloyl esterase from Aspergillus awamori G-2 strain.
For food industry production processes and other uses, a mold that produces high levels of feruloyl esterase was obtained from laboratory mold collections and other sources. It was Aspergillus awanmori G-2 that produces high levels of feruloyl esterase. The feruloyl esterase was purified using ion-exchange chromatography, size-exclusion chromatography, and HPLC chromatography. The enzyme was identified as a monomer protein using size-exclusion chromatography. Its optimum temperature and pH were, respectively, 40 degrees C and pH 5. Its activity was stable at pH 3 to 5. The enzyme was combined with xylan and starch, but it was absorbed by cellulose. The km of the feruloyl esterase was 0.0019% (0.01 mM). The enzyme showed stable activity at pH 3 and 50 degrees C, making this enzyme useful for food production.
Kanauchi
M
M
Dept. of Food Management, Miyagi Univ. 2-2-1 Hatatate, Taihaku-ku, Sendai, Miyagi, Japan 982-0215. kanauchi@myu.ac.jp
Watanabe
S
S
Tsukada
T
T
Atta
K
K
Kakuta
T
T
Koizumi
T
T
D001230:Aspergillus / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005524:Food Technology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000378:metabolism
D014990:Xylans; D002482:Cellulose; D013213:Starch; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase