On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins.
Electron capture dissociation (ECD) allows fragmentation of the phosphopeptide backbone while keeping the labile phospho-amino acid intact. This feature of ECD fragmentation, coupled with the acquisition of mass spectra at high mass accuracy, makes ECD well-suited to phosphorylation mapping. The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses.
Sweet
Steve M M
SM
University of Birmingham, School of Biosciences, Birmingham, UK.
Cooper
Helen J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D002853:Chromatography, Liquid / Q000379:methods*; D004583:Electrons; D006801:Humans; D009862:Online Systems; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software
D010748:Phosphopeptides; D010750:Phosphoproteins; D011494:Protein Kinases