title: Trifurcate feed-forward regulation of age-dependent cell death involving miR164 in Arabidopsis.
abstract: Aging induces gradual yet massive cell death in higher organisms, including annual plants. Even so, the underlying regulatory mechanisms are barely known, despite the long-standing interest in this topic. Here, we demonstrate that ORE1, which is a NAC (NAM, ATAF, and CUC) transcription factor, positively regulates aging-induced cell death in Arabidopsis leaves. ORE1 expression is up-regulated concurrently with leaf aging by EIN2 but is negatively regulated by miR164. miR164 expression gradually decreases with aging through negative regulation by EIN2, which leads to the elaborate up-regulation of ORE1 expression. However, EIN2 still contributes to aging-induced cell death in the absence of ORE1. The trifurcate feed-forward pathway involving ORE1, miR164, and EIN2 provides a highly robust regulation to ensure that aging induces cell death in Arabidopsis leaves.
authors.affiliation: Division of Molecular Life Sciences, Pohang University of Science and Technology, Hyoja-dong, Pohang, Kyungbuk, 790-784, Republic of Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Hye Ryun
authors.initials: HR
authors.lastname: Woo
authors.forename: Jeongsik
authors.initials: J
authors.lastname: Kim
authors.forename: Pyung Ok
authors.initials: PO
authors.lastname: Lim
authors.forename: In Chul
authors.initials: IC
authors.lastname: Lee
authors.forename: Seung Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Hong Gil
authors.initials: HG
authors.lastname: Nam
mesh_terms: D000375:Aging; D017209:Apoptosis; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D018515:Plant Leaves / Q000166:cytology / Q000502:physiology*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; C119963:EIN2 protein, Arabidopsis; C538852:MIRN164 microRNA, Arabidopsis; D035683:MicroRNAs; C538850:ORE1 protein, Arabidopsis; D012333:RNA, Messenger; D018749:RNA, Plant; D011956:Receptors, Cell Surface; D014157:Transcription Factors