[Effect of Semen Litchi containing serum on proliferation and apoptosis of HepG2 cells].
OBJECTIVE
To observe the effect of Semen Litchi containing serum on proliferation and apoptosis of HepG2 cells.


METHODS
The Semen litchi or CTX containing serum and control serum were prepared by serologic pharmacology method. MTT assay was used to observe the proliferation inhibition rate of HepG2 cells after incubated with different kinds of drug's containing serum. Nuclear morphological features of HepG2 cells were detected by fluorescencemicroscopy after staining with Hochest33258. The apoptosis rate of HepG2 cells in each group was detected by flow cytometry.


RESULTS
The cell viability and the apoptosis rate of HepG2 cells in Semen Litchi containing serum groups were higher than that of control group, and the results of fluorescencemicroscopy observation showed the nuclear morphological change of apoptosis.


CONCLUSIONS
Semen Litchi can inhibit the proliferation of HepG2 cells, the acting mechanism may be concerned with cell apoptosis.
Xiong
Ai-Hua
AH
School of Pharmacy, Jinan University, Guangzhou 510632, China.
Shen
Wen-Juan
WJ
Xiao
Liu-Ying
LY
Lv
Jun-Hua
JH
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D032125:Litchi / Q000737:chemistry; D008113:Liver Neoplasms / Q000473:pathology; D008297:Male; D008856:Microscopy, Fluorescence; D011817:Rabbits; D011897:Random Allocation; D012639:Seeds / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D003520:Cyclophosphamide