title: Modulation of ASK1 expression during overexpression of Trx and HSP70 in stressed fish liver mitochondria.
abstract: Mitochondrial heat shock protein 70 (mtHSP70) is found to play a primary role in cellular defense against physiological stress like exposure to environmental contaminants and helpful in the maintenance of cellular homeostasis by promoting the cell survival. In the present investigation, the environmental-stress-induced increase in mtHSP70 levels along with the quantification of apoptosis signal regulating kinase 1 (ASK1) and thioredoxin (Trx) were measured in the liver mitochondria of grey mullets (Mugil cephalus) collected from the polluted Ennore estuary and the unpolluted Kovalam estuary for a period of 2 years. The results showed elevated lipid peroxide (LPO) and decreased total antioxidant capacity along with the decrease in mitochondrial viability percentage. Mitochondrial HSP70, ASK1, and Trx levels were increased under this stress condition. A 42% increase in LPO levels and 18% decrease in mitochondrial survivality were observed in the polluted-site fish liver mitochondria when compared to the results of unpolluted estuary. We also report that, under observed oxidative stress condition in Ennore fish samples, the ASK1 levels are only moderately elevated (13% increase). This may be due to mitochondrial-HSP70-induced adaptive tolerance signaling for the activation of Trx (22% increase) which suppresses the ASK1 expression thereby promoting the cell survival that leads to the maintenance of the cellular homeostasis.
authors.affiliation: Research Department of Biochemistry, Bharathi Women's College, Chennai, 600108 Tamil Nadu, India. dstpadmini@rediffmail.com
authors.forename: Ekambaram
authors.initials: E
authors.lastname: Padmini
authors.forename: Bose
authors.initials: B
authors.lastname: Vijaya Geetha
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D015227:Lipid Peroxidation; D048848:MAP Kinase Kinase Kinase 5 / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D018384:Oxidative Stress; D023701:Smegmamorpha / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029941:Fish Proteins; D047908:Intracellular Signaling Peptides and Proteins; D048848:MAP Kinase Kinase Kinase 5