DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision.
No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus DeltaH, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus DeltaH has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus DeltaH.
Schomacher
Lars
L
Abteilung Molekulare Genetik und Präparative Molekularbiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany.
Chong
James P J
JP
McDermott
Paul
P
Kramer
Wilfried
W
Fritz
Hans-Joachim
HJ
D019843:Archaeal Proteins / Q000378:metabolism*; D011088:DNA Ligases / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D045585:Flap Endonucleases / Q000378:metabolism; D017013:Methanobacteriaceae / Q000201:enzymology*; D014498:Uracil / Q000378:metabolism*
D019843:Archaeal Proteins; D014498:Uracil; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases; D045585:Flap Endonucleases; D011088:DNA Ligases; D045643:DNA Repair Enzymes