Identification and analysis of new proteins involved in the DNA damage response network of Fanconi anemia and Bloom syndrome.
The use of co-immunoprecipitation (co-IP) to purify multi-protein complexes has contributed greatly to our understanding of the DNA damage response network associated with Fanconi anemia (FA), Bloom syndrome (BS) and breast cancer. Four new FA genes and two new protein partners for the Bloom syndrome gene product have been identified by co-IP. Here, we discuss our experience in using co-IP and other techniques to isolate and characterize new FA and BS-related proteins.
Guo
Rong
R
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, Baltimore, MD 21224, USA.
Xu
Dongyi
D
Wang
Weidong
W
D001816:Bloom Syndrome / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D004249:DNA Damage; D004264:DNA Topoisomerases, Type I / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D005199:Fanconi Anemia / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D053484:RecQ Helicases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C501527:RMI1 protein, human; C533196:RMI2 protein, human; C097151:Bloom syndrome protein; D053484:RecQ Helicases; D004264:DNA Topoisomerases, Type I