RNAi methodologies for the functional study of signaling molecules.
RNA interference (RNAi) was investigated with the aim of achieving gene silencing with diverse RNAi platforms that include small interfering RNA (siRNA), short hairpin RNA (shRNA) and antisense oligonucleotides (ASO). Different versions of each system were used to silence the expression of specific subunits of the heterotrimeric signal transducing G-proteins, G alpha i2 and G beta 2, in the RAW 264.7 murine macrophage cell line. The specificity of the different RNA interference (RNAi) platforms was assessed by DNA microarray analysis. Reliable RNAi methodologies against the genes of interest were then developed and applied to functional studies of signaling networks. This study demonstrates a successful knockdown of target genes and shows the potential of RNAi for use in functional studies of signaling molecules.
Lee
Gwang
G
Department of Molecular Science and Technology, College of Natural Science, Ajou University, Suwon, Korea.
Santat
Leah A
LA
Chang
Mi Sook
MS
Choi
Sangdun
S
D000818:Animals; D035843:Biomedical Research / Q000379:methods; D002460:Cell Line; D020868:Gene Silencing; D008264:Macrophages; D008722:Methods; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000235:genetics*
D034741:RNA, Small Interfering