title: Deregulation of microRNA involved in hematopoiesis and the immune response in HTLV-I adult T-cell leukemia.
abstract: Human T-cell leukemia virus type-I (HTLV-I) is the etiologic agent of adult T-cell leukemia (ATL), an aggressive lymphoproliferative disease. MicroRNAs (miRNAs) are differentially expressed during hematopoiesis and lineage commitment of hematopoietic stem cell progenitors (HSCPs). Here, we report aberrant expression of hematopoietic-specific miR-223, miR-181a, miR-150, miR-142.3p, and miR-155 in HTLV-I-infected cells in vitro and uncultured ex vivo ATL cells. Our results suggest that HTLV-I-infected cells have an unbalanced expression of miRNA that favors T-cell differentiation. We also found altered expression of miRNA previously recognized as innate immunity regulators: miR-155, miR-125a, miR-132, and miR-146. Strikingly, our data also revealed significant differences between ex vivo ATL tumor cells and in vitro HTLV-I cell lines. Specifically, miR-150 and miR-223 were up-regulated in ATL patients but consistently down-regulated in HTLV-I cell lines, suggesting that ATL cells and in vitro-established cells are derived from distinct cellular populations.
authors.affiliation: University of Kansas Medical Center, Department of Pathology and Laboratory Medicine, Center for Viral Oncology and University of Kansas Cancer Center, Kansas City, 66160, USA.
authors.forename: Marcia
authors.initials: M
authors.lastname: Bellon
authors.forename: Yves
authors.initials: Y
authors.lastname: Lepelletier
authors.forename: Olivier
authors.initials: O
authors.lastname: Hermine
authors.forename: Christophe
authors.initials: C
authors.lastname: Nicot
mesh_terms: D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006410:Hematopoiesis / Q000235:genetics* / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000502:physiology; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics* / Q000276:immunology*; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D035683:MicroRNAs