[Mechanism of gambogic acid-induced apoptosis in Raji cells].
This study was purposed to explore the apoptotic effect of gambogic acid on Raji cells and the role of death inducer-obliterator 1 (DIO-1) in this process. Annexin V-fluorescein-isothiocyanate/propidium iodide was used to detect apoptosis of Raji cells. Western blot was used to determine the expressions of DIO-1, Bcl-xL, pro-caspase 3 and 2 activated subunits: P17 and P20. The subcellular localization of DIO-1 in untreated and treated Raji cells was checked by immunofluorescence and Hoechst 33258 double staining. The results showed that the Gambogic acid dose-dependently induced the apoptosis of Raji cells, downregulated the expression of Bcl-xL, upregulated the expressions of DIO-1 and pro-caspase 3, induced the cleavage of pro-caspase 3 and DIO nuclear translocation. It is concluded that gambogic acid induces the apoptosis of Raji cells through DIO-1 upregulation, nuclear translocation, Bcl-xL downregulation and caspase 3 activation.
Wang
Yong
Y
Institute of Hematology, Huazhong University of Science and Technology, Wuhan 430022, China.
Chen
Yan
Y
Chen
Zi
Z
Ke
Wen-Juan
WJ
Wu
Qiu-Ling
QL
He
Jing
J
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D044004:Xanthones / Q000494:pharmacology*; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism*
C494811:BCL2L1 protein, human; C518584:DIDO1 protein, human; D004268:DNA-Binding Proteins; D044004:Xanthones; D051020:bcl-X Protein; C052659:gambogic acid; C505407:CASP3 protein, human; D053148:Caspase 3