title: A pilot study to evaluate a novel subcutaneous continuous glucose monitoring system in healthy Beagle dogs.
abstract: Serial blood glucose measurements are currently regarded as the 'gold standard' for evaluating glycaemic control of canine diabetic patients. The aim of this study was to investigate a subcutaneous continuous glucose monitoring system based on microdialysis. Analyses were performed by taking interstitial glucose samples from two different anatomical regions (interscapular region [IR], thoracic region [TR]) in six healthy Beagle dogs during induced hypoglycaemia and hyperglycaemia, the feeding period and a period of stable glucose values. For comparison, plasma glucose concentration was measured simultaneously by a hexokinase-based automated chemistry analyser. The mean absolute differences ranged from 11.7 mg/dL (SD 23.5 mg/dL, TR) to 5.3 mg/dL (SD 19.2 mg/dL, IR) with a correlation of r=0.43-0.92 (P<0.01). Sensitivity for the detection of hypoglycaemia was 85.0% TR and 93.3% IR, and the specificity was 99.5% TR and 99.7% IR, respectively. In a modified Clarke error grid analysis, 99.3% TR and 99.7% IR of the values fell into zone A and B. Collapse of the microdialysis fibre was a recurrent problem although this was easily detectable by fluctuations in system pressure. The microdialysis system reflects physiological as well as induced variations in blood glucose in a valid manner.
authors.affiliation: Clinic of Internal Medicine and Infectious Diseases, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, A-1210 Vienna, Austria. nadja.affenzeller@vu-wien.ac.at
authors.forename: Nadja
authors.initials: N
authors.lastname: Affenzeller
authors.forename: Thomas
authors.initials: T
authors.lastname: Benesch
authors.forename: Johann G
authors.initials: JG
authors.lastname: Thalhammer
authors.forename: Michael
authors.initials: M
authors.lastname: Willmann
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D003936:Diagnosis, Computer-Assisted; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis; D004285:Dogs / Q000097:blood* / Q000378:metabolism; D005260:Female; D006943:Hyperglycemia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D007003:Hypoglycemia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D008297:Male; D017551:Microdialysis / Q000379:methods / Q000662:veterinary*; D008991:Monitoring, Physiologic / Q000379:methods / Q000662:veterinary*; D010865:Pilot Projects; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose