Human amniotic mesenchymal cells differentiate into chondrocytes.
Recently, cartilage diseases have been treated by auto- or allogenic chondrocyte transplantation. However, such treatments are limited by the necessity of having a large amount of cells for transplantation, the risk of rejection, and donor shortage. Since the human amnion is immune-privileged tissue suitable for allotransplantation, the potential of human amniotic mesenchymal cells (HAMc) to differentiate into chondrocytes was assessed. The expression of gene encoding transcription factors SOXs and bone morphogenetic proteins (BMPs) as well as BMP receptors were assessed. Chondrocyte phenotype was characterized by positive expression of the cartilage marker genes collagen type II and aggrecan by RT-PCR, collagen type II protein were analyzed by immunofluorescence analysis. HAMc expressed chondrocyte-related genes, including SOXs, BMPs, as well as BMP receptors. Collagen type II and aggrecan were detected after the induction of chondrogenesis with BMP-2. HAMc, transplanted into noncartilage tissue of mice with BMP-2, or implanted with collagen-scaffold into the defects generated in a rat's bone, underwent morphological changes with deposition of collagen type II. These results showed that HAMc have the potential to differentiate into chondrocytes in vitro and in vivo, suggesting that they have therapeutic potential for the treatment of damaged or diseased cartilage.
Wei
Jun Ping
JP
Department of Organ Regeneration, The Institutes of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, Asahi, Matsumoto, Japan.
Nawata
Masashi
M
Wakitani
Shigeyuki
S
Kametani
Kiyokazu
K
Ota
Masao
M
Toda
Ayaka
A
Konishi
Ikuo
I
Ebara
Souhei
S
Nikaido
Toshio
T
D053674:Aggrecans / Q000378:metabolism; D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002357:Cartilage Diseases / Q000628:therapy; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis; D024043:Collagen Type II / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D051381:Rats; D011923:Rats, Nude; D023822:Tissue Engineering
D053674:Aggrecans; D019485:Bone Morphogenetic Proteins; D024043:Collagen Type II