title: The effect of tumor necrosis factor-alpha (TNF-alpha, interleukin (IL)-1beta and IL-6 on prostaglandins (PG)F2alpha and E2 secretion from maternal placenta in pigs.
abstract: The aim of the present study was to determine the effect of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) on prostaglandin (PG)F2alpha and PGE2 secretion as well as on cyclooxygenase-2 (COX-2) protein expression in maternal placenta collected on days 25, 30 and 40 of pregnancy in pigs. Maternal placental slices were incubated for 16 h with TNF-alpha, IL-1beta and IL-6 and (1 or 10 ng/ml of medium) or two combinations of the three cytokines (1 or 10 ng/ml of each cytokine per combination). We demonstrated the stimulatory effect of TNF-alpha, IL-1beta and IL-6 and on PGF2alpha and PGE2 secretion by the porcine maternal placenta. The medium content of these PGs depended on the cytokine type, its concentration and day of pregnancy. Cytokine stimulation of PGE2 was more pronounced than that of PGF2alpha. Additionally, an increase in PGF2alpha and/or PGE2 secretion was usually associated with the augmentation of COX-2 protein expression. Our study shows that TNF-alpha, IL-1beta and IL-6 increase production of PGF2alpha and PGE2 by porcine maternal placenta from 25, 30 and 40 day of pregnancy. These results further confirm the possible role of cytokines in modulating secretion of PGs by maternal placenta during the first trimester of gestation.
authors.affiliation: Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 0-747 Olsztyn, Poland. baja@pan.olsztyn.pl
authors.forename: B
authors.initials: B
authors.lastname: Jana
authors.forename: M
authors.initials: M
authors.lastname: Koszykowska
authors.forename: A
authors.initials: A
authors.lastname: Andronowska
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D004958:Estradiol; D015237:Dinoprost; D051546:Cyclooxygenase 2