Expression of IL-23 and IL-17 and effect of IL-23 on IL-17 production in ankylosing spondylitis.
The objective of this study was to investigate the expression of IL-23 and IL-17 and the influence of IL-23 on IL-17 production in ankylosing spondylitis (AS) patients. IL-23 and IL-17 levels in the serum and supernatants of cultured peripheral blood mononuclear cells (PBMCs) were determined by ELISA. IL-23p19 mRNA expression in PBMCs were analyzed using RT-PCR. The patients with AS at active stage showed elevated levels of IL-23 and IL-17 in the serum and supernatants of cultured PBMCs. A higher expression of IL-23p19 mRNA in PBMCs of AS patients was also observed. A significantly enhanced production of IL-17 in the supernatants of cultured PBMCs was found in the presence of recombinant IL-23 and this effect was more significant in patients with AS. The results suggest that IL-23 and IL-17 may play critical roles in the pathogenesis of AS and IL-23-stimulated production of IL-17 by PBMCs may be responsible for the development of AS.
Wang
Xinwei
X
Department of Rheumatology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, People's Republic of China. 94178-wang@163.com
Lin
Zhiming
Z
Wei
Qiujing
Q
Jiang
Yingjuan
Y
Gu
Jieruo
J
D000328:Adult; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood / Q000502:physiology*; D053759:Interleukin-23 / Q000097:blood / Q000235:genetics / Q000502:physiology*; D008297:Male; D013167:Spondylitis, Ankylosing / Q000209:etiology / Q000276:immunology*
D020381:Interleukin-17; D053759:Interleukin-23