title: Class III beta-tubulin expression in tumor cells is correlated with resistance to docetaxel in patients with completely resected non-small-cell lung cancer.
abstract: OBJECTIVE
  To assess the relationship between in vitro chemosensitivity evaluated by the histoculture drug response assay (HDRA) and the expression of beta-tubulin isotypes in tumors of patients with completely resected NSCLC in order to determine the predictive value of beta-tubulin in chemotherapy for NSCLC.
  
  
  METHODS
  Expression of beta-tubulin isotypes was immunohistochemically analyzed in a series of 58 tumor samples from patients with completely resected NSCLC. The sensitivity of individual tumors to anticancer agents was evaluated by HDRA.
  
  
  RESULTS
  Class III beta-tubulin expression by tumor cells was significantly correlated with resistance to docetaxel (p=0.0250), but not related with resistance to gemcitabine. Patient characteristics (age, gender, histology, and stage) were not associated with class III beta-tubulin expression.
  
  
  CONCLUSIONS
  An abundance of class III beta-tubulin in tumor cells could be a biomarker for resistance to docetaxel in patients with completely resected NSCLC.
authors.affiliation: Division of Respiratory Medicine, Niigata University Graduate School of Medical and Dental Sciences, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kuriyama
authors.forename: Hajime
authors.initials: H
authors.lastname: Umezu
authors.forename: Junta
authors.initials: J
authors.lastname: Tanaka
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yoshimasu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Furukawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kagamu
authors.forename: Fumitake
authors.initials: F
authors.lastname: Gejyo
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Yoshizawa
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D002478:Cells, Cultured; D000077143:Docetaxel; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D008297:Male; D043823:Taxoids / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D043823:Taxoids; D014404:Tubulin; D000077143:Docetaxel