Simultaneous immunophenotypical assessment of troponin and extracellular matrix molecules in myocardium of patients with sudden cardiac death.
In patients with sudden unexpected cardiac death, there is a relationship between the interstitial fibrosis of the myocardium and matrix molecules with a role in global remodeling of the cardiac stroma. Tissue samples of left ventricular myocardium from 17 middle-aged patients with sudden cardiac death, following acute or chronic ischemic cardio(myo)pathies, were analyzed using standard HE stain and the indirect tristadial ABC peroxidase immunohistochemical method for a panel of four antibodies involved in the dynamic remodeling of extracellular matrix: matrix metalloproteinase 9 (MMP9), tenascin X (Tn-X), TGF-b, CD54 (ICAM-1), together with simultaneously assessment of troponin in myocardic fibers. The most sensitive reaction was noticed for ICAM-1 in 71% of cases, followed by MMP9 in 59% of cases and TGF-b in 47% of cases (with great specificity for capillary vessels), in the extracellular matrix of the residual cardiomyocytes. A direct correlation, statistically significant was recorded between troponin and MMP9 (r = 0.65, p = 0.01), troponin and ICAM-1 (r = 0.31, p = 0.02), respectively ICAM-1 and tenascin (r = 0.72, p = 0.01). The extensive expression of ICAM-1 in the extracellular matrix from the perilesional area probably plays a role in the stimulation of new developing adhesion substrates between residual cells and adjacent stroma, while the over expression of troponin in the residual cardiomyocytes is accompanied by a high expression of MMP9 in the myocardic interstitium, with heterogeneous remodeling of the ventricular stroma. The simultaneous IHC expression of tenascin and ICAM-1 suggests a colocalization required for the nerve sprouting in the residual myocardium and for developing new focal cell-matrix adhesion contacts.
Ceauşu
M
M
Department of Pathology, "Victor Babeş" National Institute for Research and Development in Pathology and Biomedical Sciences, Bucharest, Romania. ceausu_mihai@yahoo.com
Curcă
C
C
Dermengiu
D
D
Ardeleanu
Carmen
C
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D016757:Death, Sudden, Cardiac / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D019063:Tenascin / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis; D014336:Troponin / Q000032:analysis*
D015703:Antigens, CD; D016326:Extracellular Matrix Proteins; D019063:Tenascin; D016212:Transforming Growth Factor beta; D014336:Troponin; C081865:tenascin X; D018799:Intercellular Adhesion Molecule-1; D020780:Matrix Metalloproteinase 9