title: Poly (I:C) induced immune response in lymphoid tissues involves three sequential waves of type I IFN expression.
abstract: An IFN-alpha heteroduplex-tracking assay (IFN-HTA) was developed to quantify the frequency of expression of the 16 genes coding for related interferon-alpha (IFN-alpha) subtypes in mice. In mLN of mice treated with Poly (I:C), we observed the induction of three sequential waves of type I IFN production, instead of two as is commonly described: early IFNs after 1 h (IFN-beta), late IFNs after 3 h (mostly IFN-alpha1, -alpha2, -alpha 4 and -alpha 5) and "secondary late IFNs" after 6 h (IFN-alpha 6T and -alpha 8/6). The late IFN wave was associated with the upregulation of the interferon regulatory factor (IRF)-7 mRNA and proteins, whereas the secondary late IFN wave was associated with a slight upregulation of IRF-8 mRNA. Type I IFNs produced in the thymus were associated with a distinct IRF mRNA expression pattern. This IFN-HTA strategy can serve as a useful tool to qualify and quantify the expression of various IFN-alpha subtypes under distinct immune responses and thus provides a first step in evaluating their function.
authors.affiliation: Laboratoire d'Immunologie, Centre de Recherche du CHUM, Hôpital Saint-Luc, Montréal, Québec, Canada.
authors.forename: Thomas
authors.initials: T
authors.lastname: Démoulins
authors.forename: Marie-Laurence
authors.initials: ML
authors.lastname: Baron
authors.forename: Nadia
authors.initials: N
authors.lastname: Kettaf
authors.forename: Ali
authors.initials: A
authors.lastname: Abdallah
authors.forename: Ehssan
authors.initials: E
authors.lastname: Sharif-Askari
authors.forename: Rafick-Pierre
authors.initials: RP
authors.lastname: Sékaly
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020180:Heteroduplex Analysis; D050839:Interferon Regulatory Factor-7 / Q000378:metabolism; D050835:Interferon Regulatory Factors / Q000378:metabolism; D016898:Interferon-alpha / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D016899:Interferon-beta / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011070:Poly I-C / Q000494:pharmacology*; D020033:Protein Isoforms / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013950:Thymus Gland / Q000276:immunology; D015854:Up-Regulation; D000099185:Interferon Regulatory Factor-8
chemical_list: D050839:Interferon Regulatory Factor-7; D050835:Interferon Regulatory Factors; D016898:Interferon-alpha; D016899:Interferon-beta; D011070:Poly I-C; D020033:Protein Isoforms; D012333:RNA, Messenger; D000099185:Interferon Regulatory Factor-8; C494235:Irf7 protein, mouse