title: Fast two-dimensional NMR spectroscopy of high molecular weight protein assemblies.
abstract: An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl (1)H-(13)C correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.
authors.affiliation: Institut de Biologie Structurale Jean-Pierre Ebel CNRS-CEA-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.
authors.forename: Carlos
authors.initials: C
authors.lastname: Amero
authors.forename: Paul
authors.initials: P
authors.lastname: Schanda
authors.forename: M Asunción
authors.initials: MA
authors.lastname: Durá
authors.forename: Isabel
authors.initials: I
authors.lastname: Ayala
authors.forename: Dominique
authors.initials: D
authors.lastname: Marion
authors.forename: Bruno
authors.initials: B
authors.lastname: Franzetti
authors.forename: Bernhard
authors.initials: B
authors.lastname: Brutscher
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boisbouvier
mesh_terms: D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins