title: Polymorphism of DsbA-L gene associates with insulin secretion and body fat distribution in Chinese population.
abstract: Disulfide-bond-A oxidoreductase-like protein (DsbA-L) has been suggested to take part in the disulfide bond formation progress of proteins, including insulin and adiponectin. Recent study has demonstrated that expression of DsbA-L was decreased in obese mice and human subject, indicating that DsbA-L might be a potential target for treatment of metabolic diseases. We investigated the association of SNP-1308G/T (rs1917760) of DsbA-L gene with metabolic diseases. 589 normal glucose tolerance (NGT) subjects and 556 type 2 diabetes (T2DM) subjects were recruited. Each group was divided into normal weight (NW) (BMI<24 kg/m(2)) subgroup and overweight/obesity (OW/OB) (BMI>/=24 kg/ m(2)) subgroup. Genotype distributions and allele frequencies of SNP (-1308G/T) in DsbA-L gene were not associated with T2DM and obesity. However, it was observed that T allele carriers had better insulin secretion function compared with non-T allele carriers in NGT-NW group, not only the first phase insulin secretion (P=0.007) but also the second phase insulin secretion (P=0.031). Multiple linear regression analysis revealed that SNP-1308G/T polymorphism (rs1917760) was independently correlated with both first and second phase insulin secretion in NGT-NW group (R(2)=0.055, P=0.007; R(2)=0.029, P=0.041). Otherwise, T carriers had more visceral fat than non-T carriers (P=0.020) in NGT-OW/OB group. In conclusion, the SNP-1308G/T (rs1917760) genotypes of DsbA-L gene might participate in insulin secretion and body fat distribution. It is possible that polymorphisms of DsbA-L gene associated with metabolic diseases.
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Clinical Medicine Center for Diabetes, Shanghai, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Qichen
authors.initials: Q
authors.lastname: Fang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Junxi
authors.initials: J
authors.lastname: Lu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Lu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Weiping
authors.initials: W
authors.lastname: Jia
authors.forename: Kunsan
authors.initials: K
authors.lastname: Xiang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000235:genetics; D015992:Body Mass Index; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance / Q000235:genetics; D000078790:Insulin Secretion; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D001786:Blood Glucose; D007328:Insulin; C487896:GSTK1 protein, human; D005982:Glutathione Transferase