Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes.
Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.
Igarashi
Aki
A
Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
Yamagata
Kousuke
K
Sugai
Tomokazu
T
Takahashi
Yukari
Y
Sugawara
Emiko
E
Tamura
Akihiro
A
Yaegashi
Hajime
H
Yamagishi
Noriko
N
Takahashi
Tsubasa
T
Isogai
Masamichi
M
Takahashi
Hideki
H
Yoshikawa
Nobuyuki
N
D017360:Arabidopsis / Q000235:genetics*; D019662:Cucurbitaceae / Q000235:genetics; D007887:Fabaceae / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D020868:Gene Silencing; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D008190:Lyases / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*
D018749:RNA, Plant; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; D008190:Lyases; C086788:magnesium chelatase