[Effects of A68930 on rhythmical respiratory discharge in isolated neonatal rat brainstem slice].
OBJECTIVE
To investigate the role of dopamine-1 receptor in the modulation of basic respiration rhythm.


METHODS
Newborn SD rat (0-3 days, n=20) brain stem slices containing the medial region of the nucleus retrofacialis (mNRF) were prepared with the hypoglossal nerve roots retained. The respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve was recorded using suction electrodes on these preparations, and the effects of dopamine-1 receptor on RRDA were investigated by application of the specific agonist of dopamine-1 receptor A68930 at different concentrations (0, 1, 2, and 5 micromol/L) in the perfusion solution.


RESULTS
The respiratory cycles (RC) and the expiratory time (TE) decreased progressively with gradual increment of the integrated amplitude (IA) after A68930 administration, and their changes were the most conspicuous at 5 min after the administration. A68930 at the concentrations of 2 and 5 micromol/L resulted in the most obvious changes in RC, TE, and IA (P<0.05), but IA exhibited no significant variation at 1 min after perfusion with 2 micromol/L A68930 (P>0.05). RC and TE were gradually shortened after treatment with increasing concentrations of A68930, which also caused gradual increment of IA, and at the concentration of 5 micromol/L, RC, TE, and IA all showed the most obvious changes (P<0.01).


CONCLUSIONS
Dopamine-1 receptor plays a role in the modulation of RRDA in isolated neonatal rat brainstem slice. A68930 may increase the frequency of respiration by shortening TE and enhance the respiratory activity by increasing the amplitude of inspiratory discharge of the respiratory neurons.
Jiao
Yong-gang
YG
Department of Physiology, Southern Medical University, Guangzhou 510515, China.
Wu
Min
M
Wu
Zhong-hai
ZH
D000818:Animals; D000831:Animals, Newborn; D002469:Cell Separation; D002839:Chromans / Q000494:pharmacology*; D018491:Dopamine Agonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011954:Receptors, Dopamine / Q000502:physiology*; D012119:Respiration / Q000187:drug effects*
D002839:Chromans; D018491:Dopamine Agonists; D011954:Receptors, Dopamine; C027460:dopamine-I receptor; C065380:A 68930