[Effect of As(2)O(3) on expressions of COX-2 and matrix metalloproteinases in SGC7901 and K562 cells].
This study was aimed to investigate on effect of As(2)O(3) on expressions of COX-2, MMP-2 and MMP-9 in SGC7901 and K562 cells. SGC7901 and K562 cells were cultured in RPMI 1640 medium and were inoculated in culture medium with different concentrations of As(2)O(3) and at different times. Expressions of COX-2, MMP-2 and MMP-9 in SGC7901 and K562 cells were measured by using Western blot, while the levels of COX-2 mRNA and MMP-2 mRNA were measured with fluorescence quantitative RT-PCR. The results showed that the expression of COX-2, MMP-2 and MMP-9 decreased in dose- and time-dependent manners after treating with As(2)O(3). The levels of COX-2 mRNA and MMP-2 mRNA reduced in groups treated with As(2)O(3). In conclusion, As(2)O(3) inhibits expressions of COX-2, MMP-2 and MMP-9 in K562 and SGC7901 cells, suggesting that As(2)O(3) inhibits tumor development through its effect on angiogenesis involved in solid and hematologic malignancies.
Xiao
Yan-Feng
YF
Department of Pediatrics, Xi'an Jiaotong University Medical College, Xi'an 710004, China. xiaoyanfeng0639@sina.com
Chen
Xi
X
Liu
Shan-Xi
SX
Wu
De-Dong
DD
Ren
Li-Fen
LF
D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*
D001152:Arsenicals; D010087:Oxides; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D000077237:Arsenic Trioxide