Cell-autonomous requirement for rx function in the mammalian retina and posterior pituitary.
Rx is a paired-like homeobox gene that is required for vertebrate eye formation. Mice lacking Rx function do not develop eyes or the posterior pituitary. To determine whether Rx is required cell autonomously in these tissues, we generated embryonic chimeras consisting of wild type and Rx-/- cells. We found that in the eye, Rx-deficient cells cannot participate in the formation of the neuroretina, retina pigment epithelium and the distal part of the optic stalk. In addition, in the ventral forebrain, Rx function is required cell autonomously for the formation of the posterior pituitary. Interestingly, Rx-/- and wild type cells segregate before the morphogenesis of these two tissues begins. Our observations suggest that Rx function is not only required for the morphogenesis of the retina and posterior pituitary, but also prior to morphogenesis, for the sorting out of cells to form distinct fields of retinal/pituitary cells.
Medina-Martinez
Olga
O
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America.
Amaya-Manzanares
Felipe
F
Liu
Chaomei
C
Mendoza
Marisela
M
Shah
Rina
R
Zhang
Li
L
Behringer
Richard R
RR
Mahon
Kathleen A
KA
Jamrich
Milan
M
D000818:Animals; D002465:Cell Movement; D002678:Chimera; D004622:Embryo, Mammalian; D005136:Eye Proteins / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009024:Morphogenesis; D010904:Pituitary Gland, Posterior / Q000196:embryology / Q000254:growth & development*; D012160:Retina / Q000196:embryology / Q000254:growth & development*
D005136:Eye Proteins; D018398:Homeodomain Proteins; C501274:Rx protein, mouse