Preparation and in vitro-in vivo evaluation of salmon calcitonin-loaded polymeric nanoparticles.
The aim of the study was to develop and characterize polymeric nanoparticles as a sustained release system for salmon calcitonin (sCT). Nanoparticles were prepared by a double emulsion solvent evaporation method using Eudragit RS and two types of a biodegradable poly(lactic-co-glycolic) copolymer (PLGA). It was demonstrated that sCT was incorporated into nanoparticles with encapsulation efficiencies in the range 69-83%. In vitro release studies, unconventionally conducted in 5% acetic acid, showed great differences in sCT release time profiles. Nanoparticles with fast release profile (Eudragit RS, PLGA/Eudragit RS) released 80-100% of the encapsulated drug within a few hours. In contrast, the sCT release from pure PLGA nanoparticles was very slow, incomplete and reached only 20% after 4 weeks. In vivo study, conducted in Wistar rats, proved that elevated serum sCT levels could be sustained for 3 days after subcutaneous administration of PLGA nanoparticles and the achieved bioavailability was increased compared to sCT solution.
Glowka
Eliza
E
Faculty of Pharmacy, Laboratory of Pharmaceutical Technology and Biopharmacy, Nancy University, Nancy Cedex, France.
Sapin-Minet
Anne
A
Leroy
Pierre
P
Lulek
Janina
J
Maincent
Philippe
P
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage* / Q000097:blood; D002116:Calcitonin / Q000008:administration & dosage* / Q000097:blood; D003692:Delayed-Action Preparations / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D055550:Protein Stability; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon
D000180:Acrylic Resins; D050071:Bone Density Conservation Agents; D003692:Delayed-Action Preparations; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; C050528:Eudragit RS; D019344:Lactic Acid; D002116:Calcitonin