A modular monolayer coating enables cell targeting by luminescent yttria nanoparticles.
Luminescent Eu(3+)-doped Y(2)O(3) nanoparticles are functionalized for cell targeting using a modular, multisegmented approach based on a phosphonate monolayer platform. The first segment provides hydrolytic stability for the particle-organic interface; the second enables aqueous suspendability; the third is used to bond cell attachment molecules. In vitro imaging experiments showed enhanced cell attachment of activated nanoparticles conjugated with cell attachment peptides compared to control nanoparticles. Peptide-derivatized nanoparticles are not displaced from the cells by their soluble peptide analogue, which suggests strong, polyvalent cell-particle interactions.
Traina
Christopher A
CA
Department of Chemistry, Princeton University, Princeton, NJ 08544-1009, USA.
Dennes
T Joseph
TJ
Schwartz
Jeffrey
J
D000818:Animals; D002448:Cell Adhesion; D005063:Europium / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry* / Q000378:metabolism; D015019:Yttrium / Q000737:chemistry* / Q000378:metabolism
D049408:Luminescent Agents; D063065:Organophosphonates; D005063:Europium; D015019:Yttrium; C091417:yttria