title: Application of open tubular capillary columns coated with zirconium phosphonate for enrichment of phosphopeptides.
abstract: A new approach utilizing open tubular capillary columns coated with zirconium phosphonate (ZrP-OTCC) for enrichment of phosphopeptides is described. The experimental conditions: interior diameter, length of capillary and flow rate was optimized using tryptic digest of alpha-casein (a phosphoprotein) as a model sample. The ZrP-OTCC was demonstrated to tolerate urea, sodium dodecyl sulphate (SDS), and NaCl. Further experimental results show that the ZrP-OTCC can trap the phosphopeptides even at the concentration of alpha-casein as low as 10(-8)M. This column has also been successfully coupled online with nano-liquid chromatography for enrichment and then separation of phosphopeptides from a complex sample, and finally analyzed the phosphopeptides by mass spectrometry (MS).
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, No. 33 Life Science Park Road, Changping District, Beijing 102206, PR China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Xue
authors.forename: Junying
authors.initials: J
authors.lastname: Wei
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Han
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhao
authors.forename: Dong
authors.initials: D
authors.lastname: Cao
authors.forename: Jinglan
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D010748:Phosphopeptides / Q000302:isolation & purification*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D010748:Phosphopeptides; C027006:zirconium phosphate; D015040:Zirconium