Enhanced amine and amino acid analysis using Pacific Blue and the Mars Organic Analyzer microchip capillary electrophoresis system.
The fluorescent amine reactive probe Pacific Blue succinimidyl ester (PB) is used for the detection of trace amounts of amines and amino acids by microchip capillary electrophoresis on the Mars Organic Analyzer (MOA). The spectral and chemical properties of PB provide a 200-fold increase in sensitivity and improved resolution compared to fluorescamine derivatization. With the use of cross injection and PB labeling, the MOA detected amino acids at concentrations as low as 75 pM (sub-parts-per-trillion). Micellar electrokinetic chromatography (MEKC) which separates PB-labeled amino acids by their hydrophobicity is also demonstrated. The optimized MEKC conditions (45 mM CHAPSO, pH 6 at 5 degrees C) effectively separated amines and 25 amino acids with enantiomeric resolution of alanine, serine, and citrulline. Samples from the Yungay Hills region in the Atacama Desert, Chile, and from the Murchison meteorite are successfully analyzed using both techniques, and amino acids are found in the parts-per-billion range. Abiotic amino acids such as beta-alanine and epsilon-aminocaprioc acid are detected along with several neutral and acidic amino acids in the Murchison sample. The Atacama Desert sample is found to contain homochiral L-alanine and L-serine indicating the presence of extant or recently extinct life.
Chiesl
Thomas N
TN
Department of Chemistry, University of California Berkeley, Berkeley, California 94720, USA.
Chu
Wai K
WK
Stockton
Amanda M
AM
Amashukeli
Xenia
X
Grunthaner
Frank
F
Mathies
Richard A
RA
D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D002845:Chromatography; D003889:Desert Climate; D046209:Electrophoresis, Microchip / Q000379:methods*; D018559:Exobiology; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D018535:Mars; D019110:Meteoroids; D008823:Micelles; D012680:Sensitivity and Specificity; D013388:Succinimides / Q000737:chemistry*
D000588:Amines; D000596:Amino Acids; D005456:Fluorescent Dyes; D008823:Micelles; C510619:Pacific Blue succinimidyl ester; D013388:Succinimides