title: Increased number of IL-2, IL-2 receptor and IL-10 positive cells in premalignant lesions of the cervix.
abstract: Previous studies have shown the involvement of the immune response in the progression of human uterine cervix cancer. The aim of this study was to determine the expression of Interleukin-2 (IL-2), IL-2 receptor (IL-2R) and Interleukin 10 (IL-10) in different grades of cervical intraepithelial neoplasias of the exocervix (CIN 1, 2 and 3), and its relationship with the serum cytokine profiles and human papilomavirus (HPV) infection status. Indirect immunofluorescence was used to study the expression of IL-2, IL-2R and IL-10 in human cervical samples from 50 patients and 9 normal controls. Serum IL-2, IL-2R and IL-10 were measured by ELISA and HPV DNA and HPV types were identified by PCR. Increased number of IL-2, IL-2R and IL-10 positive cells were observed in the cervix from patients with CIN, associated with the grades of dysplasia. A significant correlation was observed between IL-2 and IL-2R (p>0.0001), IL-2 and IL-10 (p>0.0001), as well as IL-10 and IL-2R (p>0.0001). Twenty percent of patients were HPV positive and 84% of those patients were tissue cytokine positive. These results suggest that IL-2, IL-2R and IL-10 tissue expression may play a role in the development of cervical intraepithelial dysplasias.
authors.affiliation: Laboratorio Regional de Referencia Virológica, Facultad de Medicina, Universidad del Zulia, Venezuela.
authors.forename: Raimy
authors.initials: R
authors.lastname: Mindiola
authors.forename: Diana
authors.initials: D
authors.lastname: Caulejas
authors.forename: José
authors.initials: J
authors.lastname: Núñez-Troconis
authors.forename: Mary
authors.initials: M
authors.lastname: Araujo
authors.forename: Mariela
authors.initials: M
authors.lastname: Delgado
authors.forename: Jesús
authors.initials: J
authors.lastname: Mosquera
mesh_terms: D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000175:diagnosis; D011230:Precancerous Conditions / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000276:immunology / Q000378:metabolism* / Q000821:virology
chemical_list: D004279:DNA, Viral; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D016753:Interleukin-10