A fused silica micro-electrospray tip with an electrically floating metal wire insert to achieve more stable electrospray ionization.
A new electrospray tip with a wire insert was tested and compared with the conventional bare fused silica capillary tip. The new tip combined the approach of conventional fused silica spray tips with those containing metal wires. Here, we used a floating wire so that the tips could be prepared and replaced more easily. With the conventional tip, the electrospray process became unstable and the spray current fluctuated significantly in the presence of an air bubble. When the wire-inserted tip was used under the same conditions, much less signal deterioration occurred. The superior performance of this tip over the conventional tip was attributable to its enhanced electric conduction. The new tip has great potential for improving signal stability in LC mass spectrometry.
Chung
YunJo
Y
Center for University-Wide Research Facilities, ChonBuk National University, Chonju, Republic of Korea.
Park
ChungUng
C
Kwon
Joseph
J
Kim
Sunghwan
S
D000818:Animals; D004867:Equipment Design; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010763:Phosphorylase b / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*; D014357:Trypsin / Q000378:metabolism
D010446:Peptide Fragments; D012822:Silicon Dioxide; D010763:Phosphorylase b; D014357:Trypsin