[Preparation and characterization of chlorogenic acid complete antigen].
OBJECTIVE
Characterizate the complete antigen of the chlorogenic acid.


METHODS
The chlorogenic acid was coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC. HCL The immunogen and coating antigen were characterizated by the ultraviolet scanner. The complete antigen was used to immnune the cony pig, detect the antiserum by ELISA.


RESULTS
The coupling ratio of the complete antigens was 20. The quinza pig antiserums in 1:128 in OD values were more higher than 2 times of the negative comparison.


CONCLUSIONS
The complete antigen was preparated successfully.
Xu
Guoliang
G
Jiangxi University of Traditional Chinese Medicine Modern Chinese Native Medicine Preparation Ministry of Education key laboratory, Nanchang 330004, China. xuguoliang6606@126.com
Zhang
Zengzhu
Z
Zhang
Zhuohui
Z
Zhang
Qiyun
Q
D000818:Animals; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology*; D002726:Chlorogenic Acid / Q000737:chemistry / Q000276:immunology*; D006168:Guinea Pigs; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D052179:Antigens, Plant; D012710:Serum Albumin, Bovine; D002726:Chlorogenic Acid; D010047:Ovalbumin