[Study on detection and identification of Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry].
OBJECTIVE
To detect and type Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).


METHODS
With the optimized experimental conditions, a total of 88 Salmonella strains isolated from feces of patients suffered from diarrhea were applied onto MALDI-TOF-MS and the results were compared with those obtained from serotype profiles, drug-resistance profiles and PFGE profiles.


RESULTS
The results illustrated that all strains tested were divided into 15 types by MALDI-TOF-MS at the distance level of 100. Eighty-eight strains were further break into 44 subtypes, based on the results of MALDI-TOF-MS combined with those of drug-resistance profiles. All strains were classified as 46 subtypes and 64 subtypes in combination MALDI-TOF-MS with serotype profiles and PFGE profiles, respectively. These reveal that MALDI-TOF-MS is a good method in Salmonella species typing.


CONCLUSIONS
The detection and identification of foodborne pathogenic bacteria by MALDI-TOF-MS is of good characteristics of stability and reproducibility. It is a powerful tool in detecting and identifying foodborne pathogenic bacteria.
Wang
Yeru
Y
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Cui
Shenghui
S
Li
Fengqin
F
D015373:Bacterial Typing Techniques / Q000379:methods*; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D012475:Salmonella / Q000145:classification* / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*