Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates.
Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
Baldwin
Carson D
CD
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
Howe
Gerald B
GB
Sampath
Ranga
R
Blyn
Larry B
LB
Matthews
Heather
H
Harpin
Vanessa
V
Hall
Thomas A
TA
Drader
Jared J
JJ
Hofstadler
Steve A
SA
Eshoo
Mark W
MW
Rudnick
Karl
K
Studarus
Karen
K
Moore
David
D
Abbott
Sharon
S
Janda
J Michael
JM
Whitehouse
Chris A
CA
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004269:DNA, Bacterial