Full-length sequence of genotype 3 hepatitis E virus derived from a pig in Thailand.
Hepatitis E virus (HEV) infection in pigs was investigated in two principal swine farming areas in Thailand. Anti-HEV antibodies and HEV RNA in sera were examined in 258 pigs reared on five commercial farms from age 1 to 6.5 months and sows. Overall, 167 of 258 (64.7%) pigs were positive for anti-HEV IgG, while 20 of 258 (7.75%) had detectable HEV RNA. Sequence analysis of 20 HEV isolates obtained from viremic pigs revealed that they were 92.3-100% identical to each other and had 82.2-88.2% nucleotide similarity to other reported genotype 3 isolates in 415 nucleotide sequences within ORF2 region. Further characterization by sequencing the complete genome of the Thai swine HEV isolate (named Thai-swHEV07) and phylogenetic analysis showed that Thai-swHEV07 segregated into a cluster consisting of swine isolates from Japan, Mongolia, and Kyrgyzstan within the HEV genotype 3. The Thai-swHEV07 had a genomic length of 7,229 nt excluding the polyadenylated region at 3' terminus of the genome. Comparison of Thai-swHEV07 and 27 reported strains of genotype 3 revealed 80.4-85.9% nucleotide identity, with the highest identity of 85.9% to the novel swHEV strain from Mongolia. These findings suggest that genotype 3 HEV isolates are markedly heterogeneous.
Siripanyaphinyo
Uamporn
U
Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI).
Laohasinnarong
Dusit
D
Siripanee
Juthamas
J
Kaeoket
Kampon
K
Kameoka
Masanori
M
Ikuta
Kazuyoshi
K
Sawanpanyalert
Pathom
P
D000822:Animal Husbandry; D000818:Animals; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood; D016751:Hepatitis E / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology; D013785:Thailand / Q000453:epidemiology; D014766:Viremia / Q000453:epidemiology / Q000662:veterinary / Q000821:virology
D006508:Hepatitis Antibodies; D012367:RNA, Viral