title: Bovine papillomavirus type 1 E2 protein heterodimer is functional in papillomavirus DNA replication in vivo.
abstract: Papillomaviruses are small DNA viruses that induce epithelial lesions in their host. The viral life cycle is regulated by the family of proteins encoded by the E2 open reading frame. In addition to the full-length E2 protein, the BPV-1 genome encodes two truncated E2 proteins, E2C and E8/E2, which maintain the DNA-binding-dimerization domains, but lack the activation domain. Heterodimers formed between the full-length E2 and truncated E2 proteins serve as activators of E2-dependent transcription and papillomavirus DNA replication. We show that the single activation domain of E2 is sufficient for interaction with viral helicase E1 and for initiation of DNA replication from different papillomavirus origins. Single-chain E2 heterodimer is able to activate papillomavirus DNA replication in the context of entire BPV genome in the absence of other E2 proteins. These data suggest that E2 heterodimers with single activation domain are functional in initiation of papillomavirus replication in vivo.
authors.affiliation: Institute of Technology, University of Tartu, Nooruse 1, 50411 Tartu, Estonia. reet.kurg@ut.ee
authors.forename: Reet
authors.initials: R
authors.lastname: Kurg
authors.forename: Piia
authors.initials: P
authors.lastname: Uusen
authors.forename: Toomas
authors.initials: T
authors.lastname: Sepp
authors.forename: Mari
authors.initials: M
authors.lastname: Sepp
authors.forename: Aare
authors.initials: A
authors.lastname: Abroi
authors.forename: Mart
authors.initials: M
authors.lastname: Ustav
mesh_terms: D000818:Animals; D001665:Binding Sites; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002417:Cattle; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D014764:Viral Proteins