title: The impact of daily sildenafil on levels of soluble molecular markers of endothelial function in plasma in patients with erectile dysfunction.
abstract: OBJECTIVE
  To investigate the impact of daily sildenafil on levels of soluble molecular markers of endothelial function in men with erectile dysfunction.
  
  
  METHODS
  Patients aged >18 years with erectile dysfunction of vascular etiology for >6 months, either alone or in combination with disease states strongly associated with endothelial dysfunction such as diabetes/metabolic syndrome, hypertension and coronary artery disease, received sildenafil 25 mg orally for 4 weeks. Markers of endothelial function were measured in plasma at baseline and at end of treatment using standard methods and commercially available kits.
  
  
  RESULTS
  Altogether, 112 men with mean (SD) age of 60.6 (7.3) years completed the protocol. Sildenafil 25 mg daily for 4 weeks significantly reduced endothelin-1 levels compared with baseline (2.83+/-1.63 vs 3.24+/-1.90 pg/ml, p<0.001). Significant changes were also observed for nitric oxide (35.12+/-21.14 vs 31.91+/-16.28 pmol/lt, p=0.01) and cyclic guanosine monophosphate (3.79+/-2.37 vs 2.70+/-1.34 pmol/ml, p<0.001) levels, but not for any of the other biomarkers measured. Erectile function was significantly improved.
  
  
  CONCLUSIONS
  Daily sildenafil ameliorates endothelial function as assessed by levels of biomarkers of endothelial function in patients with erectile dysfunction. This is in agreement with other studies showing similar benefits with phosphodiesterase-5 inhibitor treatment. The clinical implications of this finding warrant further investigation.
authors.affiliation: Patras University Hospital, Urology Department (4th floor), Rion, Patras 26504, Greece.
authors.forename: Angelis
authors.initials: A
authors.lastname: Konstantinopoulos
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Giannitsas
authors.forename: Anastasios
authors.initials: A
authors.lastname: Athanasopoulos
authors.forename: Dionisios
authors.initials: D
authors.lastname: Spathas
authors.forename: Petros
authors.initials: P
authors.lastname: Perimenis
mesh_terms: D000284:Administration, Oral; D015415:Biomarkers / Q000097:blood; D006152:Cyclic GMP / Q000097:blood; D004334:Drug Administration Schedule; D019332:Endothelin-1 / Q000097:blood; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000503:physiopathology; D007172:Erectile Dysfunction / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D010879:Piperazines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D011687:Purines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; D019332:Endothelin-1; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D009569:Nitric Oxide; D000068677:Sildenafil Citrate; D006152:Cyclic GMP