title: Short communication: evaluation of an on-farm test to estimate somatic cell count.
abstract: The objective of this study was to compare the results of an on-farm test, named Somaticell, with results of electronic cell counting and for milk somatic cell count (SCC) among readers. The Somaticell test correctly determined the SCC in fresh quarter milk samples. Correlation between Somaticell and electronic enumeration of somatic cells was 0.92 and kappa coefficient 0.82. Using a threshold of 205,000 cells/mL, the sensitivity and specificity for determination of intramammary infections were 91.3 and 96.0%, respectively. The SCC was greater for milk samples from which major mastitis pathogens were recovered. Minor variation among readers was observed and most likely associated with the mixing procedure. However, the final analysis indicated that this variation was not significant and did not affect the amount of samples classified as having subclinical mastitis. The on-farm test evaluated in this study showed adequate capacity of determining SCC on quarter milk samples and may be considered as an alternative for on-farm detection of subclinical mastitis.
authors.affiliation: Clinica do Leite, Department of Animal Science, University of Sao Paulo, Piracicaba, Brazil.
authors.forename: A C O
authors.initials: AC
authors.lastname: Rodrigues
authors.forename: L D
authors.initials: LD
authors.lastname: Cassoli
authors.forename: P F
authors.initials: PF
authors.lastname: Machado
authors.forename: P L
authors.initials: PL
authors.lastname: Ruegg
mesh_terms: D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000379:methods / Q000662:veterinary*; D003612:Dairying / Q000379:methods*; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology / Q000473:pathology; D008892:Milk / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity