The self-assembly of DNA Holliday junctions studied with a minimal model.
In this paper, we explore the feasibility of using coarse-grained models to simulate the self-assembly of DNA nanostructures. We introduce a simple model of DNA where each nucleotide is represented by two interaction sites corresponding to the sugar-phosphate backbone and the base. Using this model, we are able to simulate the self-assembly of both DNA duplexes and Holliday junctions from single-stranded DNA. We find that assembly is most successful in the temperature window below the melting temperatures of the target structure and above the melting temperature of misbonded aggregates. Furthermore, in the case of the Holliday junction, we show how a hierarchical assembly mechanism reduces the possibility of becoming trapped in misbonded configurations. The model is also able to reproduce the relative melting temperatures of different structures accurately and allows strand displacement to occur.
Ouldridge
Thomas E
TE
Rudolf Peierls Centre for Theoretical Physics, 1 Keble Road, Oxford OX1 3NP, United Kingdom.
Johnston
Iain G
IG
Louis
Ard A
AA
Doye
Jonathan P K
JP
D001483:Base Sequence; D045566:DNA, Cruciform / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry; D005240:Feasibility Studies; D007700:Kinetics; D008958:Models, Molecular; D009010:Monte Carlo Method; D049329:Nanostructures / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D013696:Temperature; D013816:Thermodynamics
D045566:DNA, Cruciform; D004277:DNA, Single-Stranded