Life cycle of Rickettsia slovaca in L929 cell line studied by quantitative real-time PCR and transmission electron microscopy.
Rickettsia slovaca, a member of spotted fever rickettsiae, is an agent of a mild human disease known as Tibola or Debonel. Using quantitative real-time PCR we identified the highest point of multiplication of wild and standard type (strain B) of R. slovaca on the second vs. the fourth day postinfection. Comparing both types of R. slovaca by transmission electron microscopy substantiated different cytopathological and morphological changes in infected cells and other differences, for example a slight shift of stages during the life cycle that presented in a variety of forms and localization of the studied Rickettsia within the infected cells. Our study provides a valuable insight into the pathogenicity and virulence of R. slovaca.
Boldis
Vojtech
V
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
Strus
Jasna
J
Kocianová
Elena
E
Tusek-Znidaric
Magda
M
Stefanidesová
Katarína
K
Schwarzová
Katarína
K
Kúdelová
Marcela
M
Sekeyová
Zuzana
Z
Spitalská
Eva
E
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D003870:Dermacentor / Q000382:microbiology; D005347:Fibroblasts / Q000382:microbiology*; D006801:Humans; D007739:L Cells; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins