Carbonic anhydrase activators: activation of the beta-carbonic anhydrase Nce103 from the yeast Saccharomyces cerevisiae with amines and amino acids.
The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as scCA, was investigated for its activation with amines and amino acids. scCA was poorly activated by amino acids such as l-/d-His, Phe, DOPA, Trp (K(A)s of 82-90 microM) and more effectively activated by amines such as histamine, dopamine, serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (K(A)s of 10.2-21.3 microM). The best activator was l-adrenaline, with an activation constant of 0.95 microM. This study may help to better understand the catalytic/activation mechanisms of the beta-CAs and eventually to design modulators of CA activity for similar enzymes present in pathogenic fungi, such as Candida albicans and Cryptococcus neoformans.
Isik
Semra
S
Balikesir University, Science and Art Faculty, Department of Chemistry, Balikesir, Turkey.
Kockar
Feray
F
Aydin
Meltem
M
Arslan
Oktay
O
Guler
Ozen Ozensoy
OO
Innocenti
Alessio
A
Scozzafava
Andrea
A
Supuran
Claudiu T
CT
D000596:Amino Acids / Q000737:chemistry; D002176:Candida albicans / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002384:Catalysis; D020134:Catalytic Domain; D003455:Cryptococcus neoformans / Q000201:enzymology; D015195:Drug Design; D004789:Enzyme Activation; D004837:Epinephrine / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D020033:Protein Isoforms; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014364:Tryptophan / Q000737:chemistry
D000596:Amino Acids; D020033:Protein Isoforms; D014364:Tryptophan; D002256:Carbonic Anhydrases; D004837:Epinephrine