Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta.
A laccase was purified from Trametes hirsuta. This laccase was classified as a "white" or "yellow" laccase. pH 2.4 was optimal for the oxidation of ABTS and pH 2.5 for DMP. DMP oxidation was optimal at 85 degrees C. The half-life of this laccase was 70 min at 75 degrees C, and 5 h at 65 degrees C. Non-phenolic dyes, such as Methyl Red, were oxidized by purified laccase without mediators. The enzyme was not inhibited by Cu(2+), Mn(2+), or EDTA. These are atypical laccase characteristics that make it a good candidate for theoretical and applied research.
Haibo
Zhang
Z
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Yinglong
Zhang
Z
Feng
Huang
H
Peiji
Gao
G
Jiachuan
Chen
C
D001391:Azo Compounds / Q000378:metabolism; D052160:Benzothiazoles / Q000378:metabolism; D002413:Cations, Divalent / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D010084:Oxidation-Reduction; D013451:Sulfonic Acids / Q000378:metabolism; D013696:Temperature; D055454:Trametes / Q000201:enzymology*
D001391:Azo Compounds; D052160:Benzothiazoles; D002413:Cations, Divalent; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D008670:Metals; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C008492:methyl red; D004492:Edetic Acid; D042845:Laccase