title: A single U/C nucleotide substitution changing alanine to valine in the beet necrotic yellow vein virus P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings.
abstract: Beet necrotic yellow vein virus (BNYVV) A type isolates E12 and S8, originating from areas where resistance-breaking had or had not been observed, respectively, served as starting material for studying the influence of sequence variations in BNYVV RNA 3 on virus accumulation in partially resistant sugar beet varieties. Sub-isolates containing only RNAs 1 and 2 were obtained by serial local lesion passages; biologically active cDNA clones were prepared for RNAs 3 which differed in their coding sequences for P25 aa 67, 68 and 129. Sugar beet seedlings were mechanically inoculated with RNA 1+2/RNA 3 pseudorecombinants. The origin of RNAs 1+2 had little influence on virus accumulation in rootlets. E12 RNA 3 coding for V(67)C(68)Y(129) P25, however, enabled a much higher virus accumulation than S8 RNA 3 coding for A(67)H(68)H(129) P25. Mutants revealed that this was due only to the V(67) 'GUU' codon as opposed to the A(67) 'GCU' codon.
authors.affiliation: Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Koenig
authors.affiliation: Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Loss
authors.affiliation: Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Specht
authors.affiliation: Institut für Zuckerrübenforschung, Abteilung Phytomedizin, Holtenser Landstraße 77, D-37079 Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Varrelmann
authors.affiliation: Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Lüddecke
authors.affiliation: Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Deml
mesh_terms: D000409:Alanine / Q000737:chemistry; D019943:Amino Acid Substitution; D027461:Beta vulgaris / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018517:Plant Roots / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000821:virology*; D017422:Sequence Analysis, DNA; D014633:Valine / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012367:RNA, Viral; C096035:RNAIII, Staphylococcus aureus; D014764:Viral Proteins; D014633:Valine; D000409:Alanine