A simple method for screening antimicrobial compounds with application to plant disease and fruit quality.
OBJECTIVE
It is often difficult to extrapolate information from a Petri dish and apply it to commercial applications as with antimicrobial assays. Often large volumes of commodities are used for a virtually untested protocol and result in an unnecessary expenditure of time and materials. An intermediate method, where experimental compounds could be tested directly on a specific commodity without expending large quantities of either sample or compound, would be practical and economical.


RESULTS
A method was developed that employs the use of a small experimental chamber in which pieces of natural materials (e.g. fruit) can be tested with antimicrobial compounds. This method uses a type of autoclavable incubation chamber with a filter paper base, hydrating sponge pieces and a sterile glass sample platform. The chamber offers a sterile, controlled environment and can be manipulated to serve a number of studies.


CONCLUSIONS
The chamber results are more analogous to what happens on whole fruit than the Petri dish.


CONCLUSIONS
This method is presently used to screen for antimicrobial compounds and treatments needed to control serious economic pests compromising Florida's agriculture.
Narciso
J A
JA
USDA/ARS, Citrus and Subtropical Products Laboratory, Winter Haven, FL 33881, USA. jan.narciso@ars.usda.gov
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005638:Fruit / Q000187:drug effects / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents