[In vitro cultivation and identification of epithelial cells of keratocystic odontogenic tumor].
OBJECTIVE
To establish a method of in vitro cultivation of epithelial cells of keratocystic odontogenic tumors (KCOT).


METHODS
Tissue block and enzyme digestion techniques were used for primary cultivation of KCOT cells. The cells were grown in keratinocyte-serum free medium (K-SFM). The biological characteristics of the cultivated keratinocytes were identified by phase microscopic observation and by immunohistochemistry of cytokeratin, vimentin, cytokeratin10 and 14.


RESULTS
KCOT keratinocytes could survive for 30-50 days in K-SFM after passing 2-4 generations. Cells were polygon and showed typical slabstone-like appearance. Immunostaining showed positive staining for cytokeratin antibody, and negative for vimentin.


CONCLUSIONS
KCOT epithelial cells could be serially cultured in vitro in K-SFM by techniques suggested in this study.
Pan
Shuang
S
Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Li
Tie-jun
TJ
D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002648:Child; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007573:Jaw Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009807:Odontogenic Cysts / Q000378:metabolism / Q000473:pathology*; D009808:Odontogenic Tumors / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D055815:Young Adult
D016895:Culture Media, Serum-Free