title: Classic and molecular study of Giardia duodenalis in children from a daycare center in the region of Presidente Prudente, São Paulo, Brazil.
abstract: Epidemiological studies on giardiasis by using molecular techniques such as RAPD (Randomly Amplified Polymorphic DNA) may give information on factors related to the transmission of Giardia duodenalis. The aim of this work was to assess the epidemiology of G. duodenalis in 101 children attended at a daycare center in Presidente Bernardes, SP, Brazil. After parasitological examinations in feces samples, 15 children presented cysts of G. duodenalis. Their respective parents, brothers and pets, besides the daycare center workers, also had their feces submitted to parasitological analysis. Seven mothers and nine brothers also presented G. duodenalis cysts, while fathers, daycare workers and pets (dogs) did not presented the parasite. Besides the 15 cases with G. duodenalis, other 23 children presented other enteroparasites (Entamoeba coli, Endolimax nana, Enterobius vermicularis, Ascaris lumbricoides and Trichuris trichiura). Samples of G. duodenalis cysts from children and their relatives were submitted to molecular typing by RAPD after genomic DNA extraction and amplification of a fragment of the 18S rDNA region by PCR. After examining 31 isolates of G. duodenalis (children and their respective mothers and brothers), it was concluded that the parasite transmission occurred in children, probably during daily cohabitation at the daycare center, but not at home among their relatives or pets.
authors.affiliation: Departamento de Parasitologia, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil. nairtashima@unoeste.br
authors.forename: Nair Toshiko
authors.initials: NT
authors.lastname: Tashima
authors.forename: Maria Jacira Silva
authors.initials: MJ
authors.lastname: Simões
authors.forename: Clarice Queico Fujimura
authors.initials: CQ
authors.lastname: Leite
authors.forename: Antonio
authors.initials: A
authors.lastname: Fluminhan
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Nogueira
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Malaspina
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002656:Child Day Care Centers / Q000706:statistics & numerical data*; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis; D004285:Dogs; D005243:Feces / Q000469:parasitology*; D005260:Female; D005872:Giardia / Q000145:classification / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis / Q000453:epidemiology* / Q000635:transmission; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D015995:Prevalence; D019105:Random Amplified Polymorphic DNA Technique; D012307:Risk Factors
chemical_list: D016054:DNA, Protozoan