title: Improvement of pseudojujubogenin glycosides production from regenerated Bacopa monnieri (L.) Wettst. and enhanced yield by elicitors.
abstract: Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin glycosides [(30.62 +/- 1.29) mg/g dry wt] which was 2-fold higher than that of in vitro grown plants of the same age [(16.96 +/- 1.49) mg/g dry wt]. Plantlets regenerated from 0.1 mg/l TDZ also showed a high level of pseudojujubogenin glycosides [(27.94 +/- 1.19) mg/g dry wt]. The effect of elicitor on pseudojujubogenin glycosides accumulation in B. monnieri whole plant cultures was investigated. Chitosan at 150 mg/l and yeast extract at 2 mg/ml increased the pseudojujubogenin glycosides production [(40.83 +/- 2.24) mg/g dry wt and (40.05 +/- 2.37) mg/g dry wt, respectively] after 7 days, which was 6-fold higher than in the control cultures.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Sirilak
authors.initials: S
authors.lastname: Kamonwannasit
authors.forename: Watoo
authors.initials: W
authors.lastname: Phrompittayarat
authors.forename: Kornkanok
authors.initials: K
authors.lastname: Ingkaninan
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D034681:Bacopa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D048271:Chitosan / Q000494:pharmacology; D006027:Glycosides / Q000096:biosynthesis*; D008515:Medicine, Ayurvedic; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010946:Plants, Medicinal; D012038:Regeneration / Q000502:physiology; D014315:Triterpenes / Q000378:metabolism*; D015003:Yeasts / Q000502:physiology
chemical_list: C508084:3-O-(beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl) pseudojujubogenin; D006027:Glycosides; D010937:Plant Growth Regulators; D014315:Triterpenes; D048271:Chitosan