title: Frontiers in fluorescence microscopy.
abstract: How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way scientists look at the structures of life alive.
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal. joserino@fm.ul.pt
authors.forename: José
authors.initials: J
authors.lastname: Rino
authors.forename: José
authors.initials: J
authors.lastname: Braga
authors.forename: Ricardo
authors.initials: R
authors.lastname: Henriques
authors.forename: Maria
authors.initials: M
authors.lastname: Carmo-Fonseca
mesh_terms: D000818:Animals; D005057:Eukaryotic Cells / Q000166:cytology* / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D049452:Green Fluorescent Proteins