Mass spectrometric characterization of neuropeptides.
Because of their great biological significance, neuropeptides are the subject of intensive research. Mass spectrometry (MS) is a highly informative and sensitive method used for detecting and characterizing these compounds. Successful MS analysis of neuropeptides is dependent on careful sample preparation. Herein, we present two common sample preparation strategies: direct tissue analysis and pooled tissue extraction coupled with fractionation.
Cape
Stephanie S
SS
Department of Chemistry, School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
Dowell
James A
JA
Li
Lingjun
L
D019342:Acetic Acid / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D009928:Organ Specificity; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D009479:Neuropeptides; D019342:Acetic Acid; D000432:Methanol