title: Nodavirus associated with pathological changes in adult spotted coralgroupers (Plectropomus maculatus) in Thailand with viral nervous necrosis.
abstract: The present study characterized viral nervous necrosis in sea cage-reared adult spotted coralgroupers (Plectropomus maculatus). Histopathological study showed extensive vacuolation and neuronal necrosis of the olfactory bulb and the optic lobe of the forebrain and the inner and outer nuclear layer of retina. Mild necrosis was observed in the spinal cord. Homogeneous intranuclear inclusion bodies were noted in the hyperplastic and hypertrophic glandular epithelial cells of the swim bladder suggesting viral etiology. Etiological diagnosis of VNN was confirmed by RT-PCR, immunohistochemistry and in situ hybridization. The immunohistochemistry and in situ hybridization gave strongly positive staining in the same area of the infected cells of the brain, spinal cord and retina correlating with histopathological changes. No positive reaction was detectable in the affected gas glandular epithelium and other organs, confirming the consistent neurotropism of this nodavirus. Nodavirus was mainly detected in the olfactory bulb of the brain. The result suggests nasal transmission was the major route of infection.
authors.affiliation: Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. nopadonpirarat@hotmail.com
authors.forename: Pirarat
authors.initials: P
authors.lastname: Nopadon
authors.forename: Ponpornpisit
authors.initials: P
authors.lastname: Aranya
authors.forename: Traithong
authors.initials: T
authors.lastname: Tipaporn
authors.forename: Nakai
authors.initials: N
authors.lastname: Toshihiro
authors.forename: Katagiri
authors.initials: K
authors.lastname: Takayuki
authors.forename: Maita
authors.initials: M
authors.lastname: Masashi
authors.forename: Endo
authors.initials: E
authors.lastname: Makoto
mesh_terms: D000818:Animals; D017756:Aquaculture; D005393:Fish Diseases / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D030381:Nodaviridae / Q000302:isolation & purification*; D010473:Perciformes; D012327:RNA Virus Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D013785:Thailand / Q000453:epidemiology