Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression.
We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.
Magari
Masaki
M
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
Aya
Takahiro
T
Ikeda
Mika
M
Todo
Kagefumi
K
Kanayama
Naoki
N
Ohmori
Hitoshi
H
D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D017931:DNA Primers; D051757:PAX5 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D051757:PAX5 Transcription Factor