title: Porcine skin-derived stem cells can serve as donor cells for nuclear transfer.
abstract: Although transgenic animal production through somatic cell nuclear transfer (SCNT) has been successful, the process is still inefficient. One major limitation is the use of somatic donor cells that have a finite life span. Identification and isolation of a cell type capable of rapid proliferation while possessing immortal or prolonged life span in culture and is capable of being genetically modified would be very valuable for utilization in the production of genetically modified pigs. Here we report the birth of live piglets after cloning by using porcine skin-derived stem cells (SSC) as a donor cell type. In the present study, cell cycle analysis indicates that the porcine SSC proliferate rapidly in vitro. The porcine SSC are capable of producing live offspring and can be genetically modified with positive selection. Utilization of porcine SSC may prove to be an excellent cell type for genetic modification followed by nuclear transfer for the production of transgenic pigs.
authors.affiliation: University of Missouri, Columbia, 65211, USA.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Hao
authors.forename: David
authors.initials: D
authors.lastname: Wax
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Zhong
authors.forename: Clifton
authors.initials: C
authors.lastname: Murphy
authors.forename: Jason W
authors.initials: JW
authors.lastname: Ross
authors.forename: August
authors.initials: A
authors.lastname: Rieke
authors.forename: Melissa
authors.initials: M
authors.lastname: Samuel
authors.forename: Lee
authors.initials: L
authors.lastname: Spate
authors.forename: Paul
authors.initials: P
authors.lastname: Dyce
authors.forename: Julang
authors.initials: J
authors.lastname: Li
authors.forename: Peter
authors.initials: P
authors.lastname: Sutovsky
authors.forename: Randall S
authors.initials: RS
authors.lastname: Prather
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D019976:Cloning, Organism / Q000379:methods*; D018895:Microsatellite Repeats; D046529:Microscopy, Electron, Transmission; D053652:Nuclear Transfer Techniques; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine / Q000235:genetics*