title: Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves.
abstract: Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse and human aorta. We used CD11c promoter-enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs and study their antigen-presenting capacity. Stellate EYFP(+) cells were readily identified in the aorta and could be double labeled with antibodies to CD11c and antigen-presenting major histocompatability complex (MHC) II products. The DCs proved to be particularly abundant in the cardiac valves and aortic sinus. In all aortic locations, the CD11c(+) cells localized to the subintimal space with occasional processes probing the vascular lumen. Aortic DCs expressed little CD40 but expressed low levels of CD1d, CD80, and CD86. In studies of antigen presentation, DCs selected on the basis of EYFP expression or binding of anti-CD11c antibody were as effective as DCs similarly selected from the spleen. In particular, the aortic DCs could cross-present two different protein antigens on MHC class I to CD8(+) TCR transgenic T cells. In addition, after intravenous injection, aortic DCs could capture anti-CD11c antibody and cross-present ovalbumin to T cells. These results indicate that bona fide DCs are a constituent of the normal aorta and cardiac valves.
authors.affiliation: Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Do
authors.forename: Cheolho
authors.initials: C
authors.lastname: Cheong
authors.forename: Hyein
authors.initials: H
authors.lastname: Koh
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Boscardin
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Oh
authors.forename: Leonia
authors.initials: L
authors.lastname: Bozzacco
authors.forename: Christine
authors.initials: C
authors.lastname: Trumpfheller
authors.forename: Chae Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Steinman
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001011:Aorta / Q000166:cytology* / Q000276:immunology*; D001426:Bacterial Proteins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002465:Cell Movement; D045142:Cross-Priming / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006351:Heart Valves / Q000166:cytology* / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012850:Sinus of Valsalva / Q000166:cytology / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D015415:Biomarkers; D039521:CD11c Antigen; D015395:Histocompatibility Antigens Class I; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria