title: Histopathological examination of ovaries in bitches after experimental zearalenone mycotoxicosis.
abstract: The present study deals with the influence of experimental ZEA mycotoxicosis on histopathological lesions in ovaries of bitches, which were administered zearalenone per os during anestrus phase for one hundred days. The experiment was performed on 9 sexually mature, clinically healthy bitches. The animals assigned into two experimental groups received zearalenone per os at two doses, 25 microg/kg b.w. and 50 microg/kg b.w., respectively: the bitches from control group received placebo per os. On the last day of zearalenone intoxication, the bitches were ovariohystorectomized. Histopathology and immunohistochemistry were performed. The study revealed that zearalenone and its metabolites caused profound regressive lesions: granular cells degeneration and atrophy. Numerous edemas and blood extravasations were also found. The intensity of these changes was significantly dose dependent. Furthermore, in ovarian cells and tissues of both experimental groups, no reaction for PCNA antigen was observed. In conclusion, zearalenone and its metabolites exerts unfavorable effects on the morphology of ovaries in bitches.
authors.affiliation: Division of Veterinary Prophylaxis and Feed Hygiene, Department of Veterinary Protection of Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland. mgaja@uwm.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Gajecka
authors.forename: K
authors.initials: K
authors.lastname: Obremski
authors.forename: E
authors.initials: E
authors.lastname: Jakimiuk
authors.forename: E
authors.initials: E
authors.lastname: Skorska-Wyszyńska
authors.forename: L
authors.initials: L
authors.lastname: Zielonka
authors.forename: M
authors.initials: M
authors.lastname: Gajecki
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004283:Dog Diseases / Q000139:chemically induced* / Q000473:pathology; D004285:Dogs; D005260:Female; D015651:Mycotoxicosis / Q000473:pathology / Q000662:veterinary*; D010049:Ovarian Diseases / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*; D010053:Ovary / Q000187:drug effects* / Q000473:pathology; D015025:Zearalenone / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D015025:Zearalenone