Renin-angiotensin gene polymorphisms in relation to severe chronic periodontitis.
OBJECTIVE
Evidence suggests that the ultimate product of the renin-angiotensin system (RAS), angiotensin II, exerts inflammatory actions. The present study aimed to evaluate the inter-relation between gene polymorphisms of the RAS components; angiotensin converting enzyme (ACE), angiotensinogen (AGT) and angiotensin II type-I receptor (AT1R), and severe chronic periodontitis (CP).


METHODS
DNA was obtained from peripheral blood of 90 CP patients and 126 periodontally healthy subjects, and the clinical parameters were recorded. ACE I/D, AGT M235T and AT1R A1166C polymorphisms were genotyped by the PCR-RFLP method. Chi-square, anova and logistic regression methods were used in statistical analyses.


RESULTS
The frequency of the ACE D allele was significantly lower in the CP group than the healthy group (p(corr)=0.015). CP subjects exhibited increased C allele carriage and C allele frequency of the AT1R gene (p(corr)=0.03 and p(corr)=0.03, respectively). All clinical parameters of CP patients were found to be similar in variant allele-carrying and non-carrying subjects (p>0.05).


CONCLUSIONS
The present findings suggest that ACE I/D and AT1R polymorphisms might be associated with susceptibility to CP but not with disease severity. The D allele of ACE I/D might be associated with decreased, whereas the C variant of AT1R A1166C might be associated with an elevated risk for CP in Turkish population.
Gürkan
Ali
A
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey. ali.gurkan@ege.edu.tr
Emingil
Gülnur
G
Saygan
Buket Han
BH
Atilla
Gül
G
Köse
Timur
T
Baylas
Haluk
H
Berdeli
Afig
A
D000328:Adult; D000704:Analysis of Variance; D000808:Angiotensinogen / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D055113:Chronic Periodontitis / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D012016:Reference Values; D012084:Renin-Angiotensin System / Q000235:genetics*; D012720:Severity of Illness Index; D014421:Turkey
D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; D007703:Peptidyl-Dipeptidase A