Tumor necrosis factor-alpha mediates hyperglycemia-augmented gut barrier dysfunction in endotoxemia.
OBJECTIVE
To examine whether hyperglycemia would augment gut barrier dysfunction and inflammatory responses in endotoxemic rats, and simultaneously to clarify the roles of tumor necrosis factor (TNF)-alpha in alterations of gut mucosal permeability associated with hyperglycemia.


METHODS
Prospective randomized animal study.


METHODS
University research laboratory.


METHODS
: Male Wistar rats treated with lipopolysaccharide (LPS) injection.


METHODS
After LPS injection (4 mg/kg), rats were randomly allocated into group S (n = 6), group G (n = 7), or group GI (n = 8) with continuous infusion of different fluid solutions: normal saline, 40% glucose or 10% glucose mixed with insulin, respectively. Blood glucose, insulin, and proinflammatory cytokines, accompanied by gut mucosal permeability using an in situ loop preparation of gut with fluorescence isothiocyanate-conjugated dextran, were measured. Bacterial growth or alterations in mesenteric lymph nodes and cecal contents were also assessed. We further determined the roles of TNF-alpha using an inhibitor of TNF-alpha converting enzyme in gut barrier dysfunction under the same experimental settings.


RESULTS
Hyperglycemia over 400 mg/dL was achieved and kept in group G during the study period whereas normoglycemia was preserved in group S and GI, the latter of which showed the similar extent of hyperinsulinemia to group G. Plasma concentrations of fluorescence-labeled dextran and TNF-alpha in group G were significantly higher vs. group S and GI, and the number of bacteria found in mesenteric lymph nodes in group G was greater compared with group S. Intestinal environments including microflora and organic acids were not altered by blood glucose or insulin level. Inhibiting conversion of membrane-bound to soluble type of TNF-alpha restored gut mucosal permeability augmented by hyperglycemia.


CONCLUSIONS
These findings indicate that hyperglycemia deteriorates LPS-elicited gut barrier dysfunction and bacterial translocation independently of plasma insulin level, and that TNF-alpha mediates such mucosal dysfunction of gut in endotoxemia.
Yajima
Satoshi
S
Departments of Anesthesiology, Keio University School of Medicine, Tokyo, Japan.
Morisaki
Hiroshi
H
Serita
Ryohei
R
Suzuki
Takeshi
T
Katori
Nobuyuki
N
Asahara
Takashi
T
Nomoto
Koji
K
Kobayashi
Fujio
F
Ishizaka
Akitoshi
A
Takeda
Junzo
J
D000818:Animals; D019446:Endotoxemia / Q000097:blood / Q000150:complications / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D006943:Hyperglycemia / Q000097:blood / Q000150:complications / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D008297:Male; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D014409:Tumor Necrosis Factor-alpha