title: DNA identification of biological traces and interpretation in a sexual assault case.
abstract: The approaches in research and practical work in the forensic laboratories of DNA profiling in a sexual assault case can be assessed by the individual valuation of the quality and quantity of available DNA and the choice of combination of the STRs loci that have been examined. The applied procedures are classic or modificated extraction, amplification, and electrophoretical procedures, which have been selected for every individual case. We set up to present a criminal case of sexual assault over a woman, where the suspect used a condom which--after being examined--gave a negative sperm cytologic result. Our aim is to put some debatable issues and to propose an interpretation of the obtained results based on an association of the DNA typing of the victim and the suspect, separated only by the condom.
authors.affiliation: Department of Forensic Medicine and Deontology, Medical University, Faculty of Medicine, Sofia, Bulgaria. alexa2000@mail.bg
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Apostolov
authors.forename: Stanislav
authors.initials: S
authors.lastname: Hristov
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Angelova
mesh_terms: D000328:Adult; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D017280:Condoms; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011902:Rape; D013048:Specimen Handling; D013094:Spermatozoa / Q000166:cytology; D020080:Tandem Repeat Sequences
chemical_list: D005819:Genetic Markers; D004247:DNA