[Sequence analysis of the nucleocapsid gene and genome promoter region of peste des petits ruminants virus of Chinese origin].
The N gene and genome promoter nucleotide sequence of a Chinese Peste des petits rumiants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The length of N gene was 1689 nucleotides with a single open reading frame (ORF). The nucleotide and deduced amino acid sequence was compared with the homologous region of other PPRV isolates. The nucleotide sequence of the "China/Tib/Gej/07-30" was 91.7%-97.6% identical to other PPRV isolates, while a homology of 94.9%-98.5% could be observed at the amino acids level. The N gene encoded a protein of 525 amino acids. Several sequence motifs were identified on the basis of conservation in the PPRVs and the morbilliviruses. The genome length of promoter region was 107 nucleotides with 91.8%-98.2% identity to other PPRV isolates. Phylogenetic analysis showed that the "China/Tib/Gej/07-30" belonged to the Asian lineage.
Bao
Jing-Yue
JY
National Diagnostic Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
Wang
Zhi-Liang
ZL
Li
Lin
L
Zhao
Wen-Ji
WJ
Suo
Long-Ci-Ren
LC
Li
Jin-Ming
JM
Wang
Ying-Li
YL
Wu
Xiao-Dong
XD
Liu
Chun-Ju
CJ
Liu
Yu-Tian
YT
Yu
Xiao-Jing
XJ
Yang
Yong-Mei
YM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D005260:Female; D016679:Genome, Viral; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics*; D029021:Peste-des-Petits-Ruminants / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017421:Sequence Analysis
D019590:Nucleocapsid Proteins