Internal dynamics of a living cell nucleus investigated by dynamic light scattering.
Recent progresses in cellular biology have shown that the nucleus of a living cell is a structured integration of many functional domains with a complex spatial organization. This organization, as well as molecular and biochemical processes, is time regulated. In the past years many investigations have been performed using fluorescent microscopy techniques to study the internal dynamics of the nucleus of a living cell. These investigations, however, have never focussed on the global internal dynamics of the nucleus, which is still unknown. In this article we present an original light scattering experimental device that we built to investigate this dynamics during biological processes. By means of this experimental set-up, we investigated the global dynamics of the nucleus of a living cell treated with a DNA replication inhibitor. This dynamics presents different and independent kinds of relaxation well separated in time that vary as a function of the cell cycle phases.
Suissa
M
M
Université de Lyon, Laboratoire de Physique, Ecole Normale Supérieure de Lyon, CNRS, 46 allée d'Italie, 69364 Lyon, France.
Place
C
C
Goillot
E
E
Freyssingeas
E
E
D000818:Animals; D001671:Biochemistry / Q000379:methods; D001703:Biophysics / Q000379:methods*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*; D012542:Scattering, Radiation
D005456:Fluorescent Dyes; D004247:DNA