title: [Preparation and characterization of chlorogenic acid complete antigen].
abstract: OBJECTIVE
  Characterizate the complete antigen of the chlorogenic acid.
  
  
  METHODS
  The chlorogenic acid was coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC. HCL The immunogen and coating antigen were characterizated by the ultraviolet scanner. The complete antigen was used to immnune the cony pig, detect the antiserum by ELISA.
  
  
  RESULTS
  The coupling ratio of the complete antigens was 20. The quinza pig antiserums in 1:128 in OD values were more higher than 2 times of the negative comparison.
  
  
  CONCLUSIONS
  The complete antigen was preparated successfully.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine Modern Chinese Native Medicine Preparation Ministry of Education key laboratory, Nanchang 330004, China. xuguoliang6606@126.com
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xu
authors.forename: Zengzhu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhuohui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology*; D002726:Chlorogenic Acid / Q000737:chemistry / Q000276:immunology*; D006168:Guinea Pigs; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D052179:Antigens, Plant; D012710:Serum Albumin, Bovine; D002726:Chlorogenic Acid; D010047:Ovalbumin