Molecular phenotyping of mannosyltransferases-deficient Candida albicans cells by high-resolution magic angle spinning NMR.
The yeast Candida albicans is an opportunistic pathogen that causes infections in immunocompromised individuals with a high morbidity and mortality levels. Recognition of yeasts by host cells is directly mediated by cell wall components of the yeast, including a wide range of abundantly expressed glycoconjugates. Of particular interest in C. albicans are the beta-mannosylated epitopes that show a complex expression pattern on N-glycan moiety of phosphopeptidomannans and are absent in the non-pathogenic species Saccharomyces cerevisiae. Being known as potent antigens for the adaptive immune response and elicitors of specific infection-protective antibodies, the exact delineation of beta-mannosides regulation and expression pathways has lately become a major milestone toward the comprehension of host-pathogen interplay. Using the newly developed HR-MAS NMR methodology, we demonstrate the possibility of assessing the general profiles of cell-surface-exposed glycoconjugates from intact living yeast cells without any prior purification step. This technique permitted to directly observe structural modifications of surface expressed phosphodiester-linked beta-mannosides on a series of deletion strains in beta-mannosyltransferases and phospho-mannosyltransferases compared with their parental strains.
Maes
Emmanuel
E
Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, IFR 147, Université des Sciences et Technologies de Lille 1, 59655, Villeneuve d'Ascq, France.
Mille
Céline
C
Trivelli
Xavier
X
Janbon
Guilhem
G
Poulain
Daniel
D
Guérardel
Yann
Y
D002176:Candida albicans / Q000166:cytology* / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification; D008364:Mannosyltransferases / Q000172:deficiency*; D008958:Models, Molecular; D010641:Phenotype; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry; D012995:Solubility
D008351:Mannans; D010748:Phosphopeptides; D011134:Polysaccharides; C072108:phosphopeptidomannan; D008364:Mannosyltransferases