Endosperm-specific expression of tyramine N-hydroxycinnamoyltransferase and tyrosine decarboxylase from a single self-processing polypeptide produces high levels of tyramine derivatives in rice seeds.
The plant-specific tyramine derivatives, feruloyltyramine (FT) and 4-coumaroyltyramine (CT), represent bioactive compounds found at low levels in many plant species. We generated transgenic rice seeds that produce high levels of CT (14 microg g(-1) seeds) and FT (2.7 microg g(-1) seeds) through the dual expression of tyramine N-hydroxycinnamoyltransferase and tyrosine decarboxylase, using the self-processing foot-and-mouth disease virus 2A sequence and the endosperm-specific prolamin promoter.
Park
Sangkyu
S
Department of Biotechnology, Interdisciplinary Program for Bioenergy and Biomaterials of Graduate School, Agricultural Plant Stress Research Center, Chonnam National University, Gwangju 500-757, Korea.
Kang
Kiyoon
K
Kim
Young Soon
YS
Back
Kyoungwhan
K
D000217:Acyltransferases / Q000096:biosynthesis*; D003373:Coumaric Acids / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D012275:Oryza / Q000201:enzymology* / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000378:metabolism; D014439:Tyramine / Q000031:analogs & derivatives / Q000378:metabolism*; D014445:Tyrosine Decarboxylase / Q000096:biosynthesis*; D014764:Viral Proteins / Q000235:genetics
D003373:Coumaric Acids; C001411:N-(4-hydroxy-beta-phenethyl)-4-hydroxycinnamide; D010940:Plant Proteins; D014764:Viral Proteins; C057421:virus protein 2A; C074004:feruloyltyramine; D000217:Acyltransferases; C108168:hydroxycinnamoyl-CoA-tyramine N-(hydroxycinnamoyl)transferase; D014445:Tyrosine Decarboxylase; D014439:Tyramine