title: Genetic diversity and physiological traits of Brettanomyces bruxellensis strains isolated from Tuscan Sangiovese wines.
abstract: Eighty four isolates of Brettanomyces bruxellensis, were collected during fermentation of Sangiovese grapes in several Tuscan wineries and characterized by restriction analysis of 5.8S-ITS and species-specific PCR. The isolates were subsequently analysed, at strain level, by the combined use of the RAPD-PCR assay with primer OPA-02 and the mtDNA restriction analysis with the HinfI endonuclease. This approach showed a high degree of polymorphism and allowed to identify seven haplotypes, one of them being the most represented and widely distributed (72 isolates, 85.7%). Physiological traits of the yeasts were investigated under a wine model condition. Haplotypes clustered into two groups according to their growth rates and kinetics of production of 4-ethylphenol and 4-ethylguaiacol. Hexylamine was the biogenic amine most produced (up to 3.92 mg l(-1)), followed by putrescine and phenylethylamine. Formation of octapamine was detected by some haplotypes, for the first time.
authors.affiliation: Dipartimento di Biologia delle Piante Agrarie, Università di Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Agnolucci
authors.forename: I
authors.initials: I
authors.lastname: Vigentini
authors.forename: G
authors.initials: G
authors.lastname: Capurso
authors.forename: A
authors.initials: A
authors.lastname: Merico
authors.forename: A
authors.initials: A
authors.lastname: Tirelli
authors.forename: C
authors.initials: C
authors.lastname: Compagno
authors.forename: R
authors.initials: R
authors.lastname: Foschino
authors.forename: M
authors.initials: M
authors.lastname: Nuti
mesh_terms: D055168:Brettanomyces / Q000235:genetics* / Q000502:physiology*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D021901:DNA, Intergenic; D014644:Genetic Variation; D007558:Italy; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014920:Wine / Q000145:classification* / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic