title: Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis.
abstract: Exit from mitosis is characterized by a precipitous decline in cyclin-dependent kinase (Cdk) activity, dissolution of mitotic structures, and cytokinesis. In Saccharomyces cerevisiae, mitotic exit is driven by a protein phosphatase, Cdc14, which is in part responsible for counteracting Cdk activity. Throughout interphase, Cdc14 is sequestered in the nucleolus, but successful anaphase activates the mitotic exit network (MEN), which triggers dispersal of Cdc14 throughout the cell by a mechanism that has remained unknown. In this study, we show that a MEN component, protein kinase Dbf2-Mob1, promotes transfer of Cdc14 to the cytoplasm and consequent exit from mitosis by direct phosphorylation of Cdc14 on serine and threonine residues adjacent to a nuclear localization signal (NLS), thereby abrogating its NLS activity. Our results define a mechanism by which the MEN promotes exit from mitosis.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. mohld@caltech.edu
authors.forename: Dane A
authors.initials: DA
authors.lastname: Mohl
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Huddleston
authors.forename: Therese S
authors.initials: TS
authors.lastname: Collingwood
authors.forename: Roland S
authors.initials: RS
authors.lastname: Annan
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Deshaies
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D020090:Chromosome Segregation; D003593:Cytoplasm / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D008938:Mitosis; D009154:Mutation; D019913:Nuclear Localization Signals; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism
chemical_list: C074860:CDC14 protein, S cerevisiae; C082279:CDC15 protein; D018797:Cell Cycle Proteins; C110689:MOB1 protein, S cerevisiae; D019913:Nuclear Localization Signals; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C085768:DBF2 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C503885:CDC14 protein, S pombe; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D019204:GTP-Binding Proteins