title: Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.
abstract: The mechanisms ensuring specific incorporation of CENP-A at centromeres are poorly understood. Mis16 and Mis18 are required for CENP-A localization at centromeres and form a complex that is conserved from fission yeast to human. Fission yeast sim1 mutants that alleviate kinetochore domain silencing are defective in Scm3(Sp), the ortholog of budding yeast Scm3(Sc). Scm3(Sp) depends on Mis16/18 for its centromere localization and like them is recruited to centromeres in late anaphase. Importantly, Scm3(Sp) coaffinity purifies with CENP-A(Cnp1) and associates with CENP-A(Cnp1) in vitro, yet localizes independently of intact CENP-A(Cnp1) chromatin and is differentially released from chromatin. While Scm3(Sc) has been proposed to form a unique hexameric nucleosome with CENP-A(Cse4) and histone H4 at budding yeast point centromeres, we favor a model in which Scm3(Sp) acts as a CENP-A(Cnp1) receptor/assembly factor, cooperating with Mis16 and Mis18 to receive CENP-A(Cnp1) from the Sim3 escort and mediate assembly of CENP-A(Cnp1) into subkinetochore chromatin.
authors.affiliation: Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, 6.34 Swann Building, Edinburgh EH93JR, Scotland, UK. alison.pidoux@ed.ac.uk
authors.forename: Alison L
authors.initials: AL
authors.lastname: Pidoux
authors.forename: Eun Shik
authors.initials: ES
authors.lastname: Choi
authors.forename: Johanna K R
authors.initials: JK
authors.lastname: Abbott
authors.forename: Xingkun
authors.initials: X
authors.lastname: Liu
authors.forename: Alexander
authors.initials: A
authors.lastname: Kagansky
authors.forename: Araceli G
authors.initials: AG
authors.lastname: Castillo
authors.forename: Georgina L
authors.initials: GL
authors.lastname: Hamilton
authors.forename: William
authors.initials: W
authors.lastname: Richardson
authors.forename: Juri
authors.initials: J
authors.lastname: Rappsilber
authors.forename: Xiangwei
authors.initials: X
authors.lastname: He
authors.forename: Robin C
authors.initials: RC
authors.lastname: Allshire
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D018386:Kinetochores / Q000378:metabolism*; D009154:Mutation; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; C410349:Cnp1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins