[Expression of CD45 in the serum of patients with Crohn's disease].
OBJECTIVE
To search for differentially expressed proteins in the serum of patients with Crohn's disease.


METHODS
Serum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins.


RESULTS
2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn's disease compared with that in normal adults (P<0.05). The protein was identified as CD45 using mass spectrometry.


CONCLUSIONS
CD45 overexpression in the serum of patients with Crohn's disease may play a role in the disequilibrium of the immune system.
Kang
Liang
L
Department of Colorectal Surgery, Sixth Affiliated Hospital of Sun Yat-sen University, Laboratory of Proteomics, School of Preclinical Medicine, Guangzhou 510655, China.
Yang
Zu-li
ZL
Wang
Lei
L
Huang
Mei-jin
MJ
Liu
Wei
W
Li
Ming-tao
MT
Wang
Jian-ping
JP
D000595:Amino Acid Sequence; D016022:Case-Control Studies; D003424:Crohn Disease / Q000097:blood* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood*; D008297:Male; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017493:Leukocyte Common Antigens