Inhibition of oxidant-induced biochemical changes of pork myofibrillar protein by hydrolyzed potato protein.
The objective of the study was to investigate the role of hydrolyzed potato protein (HPP) in protecting myofibril protein isolate (MPI) from oxidative modification. MPI prepared from pork muscle was suspended (30 mg protein/mL) in 15 mM piperazine-N, N-bis(2-ethane sulfonic acid) buffer (pH 6.0) with 0, 0.3, 0.75, and 1.5 mg/mL of antioxidative HPP (1-h Alcalase hydrolysate). Oxidation was induced by incubating the protein suspensions at 4 degrees C for 24 h with (1) an iron-catalyzed oxidizing system (IOS: 0.01 mM FeCl3, 0.1 mM ascorbic acid, and 1.0 mM H202) and (2) a metmyoglobin-oxidizing system (MOS: 0.1 mM metmyoglobin and 0.1 mM H2O2). Changes in oxidized MPI were measured as thiobarbituric acid-reactive substances (TBARS), protein carbonylcontent, Ca- and K-ATPase activities, and ultraviolet (UV) spectra. Oxidation increased the production of TBARS and protein carbonyls by 2.9- and 0.24-fold in IOS and 5.6- and 2.2-fold in MOS, respectively. The 2 oxidizing systems altered the Ca- and K-ATPase activities and exposed hydrophobic groups buried in MPI. The presence of HPP reduced the extent of MPI oxidation in all physicochemical categories tested. Therefore, HPP may be used as a potential functional ingredient in meat products to enhance their oxidative stability.
Wang
L L
LL
Dept. of Animal and Food Sciences, Univ. of Kentucky, Lexington, KY 40546, USA.
Xiong
Y L
YL
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002384:Catalysis; D006868:Hydrolysis; D007501:Iron / Q000378:metabolism; D008786:Metmyoglobin / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000378:metabolism; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000737:chemistry*; D013552:Swine; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D009124:Muscle Proteins; D016877:Oxidants; D010940:Plant Proteins; D017392:Thiobarbituric Acid Reactive Substances; D008786:Metmyoglobin; D007501:Iron