Inhibition of thrombin and factor Xa by Fucus evanescens fucoidan and its modified analogs.
Specimens of fucoidan extracted from Fucus evanescens were purified from protein and polyphenols, deacetylated and depolymerized by fucoidanase for evaluation of their biological activity. Deacetylation did not modify the capacity of fucoidan to inhibit thrombin and factor Xa, while purification from protein and polyphenols reduced this capacity. Depolymerization of fucoidan increased its capacity to inhibit thrombin mainly through heparin cofactor II. All the studied specimens formed complexes with protamine sulfate.
Lapikova
E S
ES
Laboratory of Hemostasis Pathology and Pharmacology, Hematology Research Center, Russian Academy of Medical Sciences, Moscow.
Drozd
N N
NN
Tolstenkov
A S
AS
Makarov
V A
VA
Zvyagintseva
T N
TN
Shevchenko
N M
NM
Bakunina
I U
IU
Besednova
N N
NN
Kuznetsova
T A
TA
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D065427:Factor Xa Inhibitors; D044645:Fucus / Q000737:chemistry; D008970:Molecular Weight; D011134:Polysaccharides / Q000494:pharmacology*; D011817:Rabbits; D013917:Thrombin / Q000037:antagonists & inhibitors*
D000925:Anticoagulants; D065427:Factor Xa Inhibitors; D011134:Polysaccharides; C007789:fucoidan; D013917:Thrombin