Age-dependent differential expression of genes involved in steroid signalling pathway in the brain of protandrous black porgy, Acanthopagrus schlegeli.
The mechanisms underlying brain sex differentiation in animals are poorly understood. In the present study, using black porgy, Acanthopagrus schlegeli, as primary experimental model, we investigated the temporal expression patterns of receptors for androgen (ar) and estrogen (esr1 and esr2a) in the brain during posthatching ages and analyzed them against the timing of gonadal germ cell development. We hypothesized that endogenous estrogens naturally masculinize the brain of black porgy. The expression of sex steroid receptors was studied in relation to a wider suite of other related genes (nr5a2, nr0b1, star, and cyp19a1b) to provide some insight into the monomale sex differentiation pattern observed in this species. Our results revealed a highly significant increase in esr1 together with the increase in esr2a at 120 dph (days posthatching), suggesting a significant role for esr in sex differentiation in this species. Temporal expression patterns of nr5a2, nr0b1, star, sex steroid receptors, and cyp19a1b in the brain provided evidence for their physiological roles in the monomale sex differentiation in this species. The expression of nr5a2, star, ar, esr1, esr2a, and cyp19a1b increased at 120 dph, a period when brain sex differentiation probably occurs in this species. The study also suggests that neurosteroidogenesis in black porgy may be regulated by both nr5a2-dependent and nr5a2-independent mechanisms. The results demonstrated striking differences in the abundance of the gene transcripts in discrete brain region throughout ontogeny. In addition, the sex steroid hormone levels and aromatase activity in brain at different developmental states and the changes in the gene expression patterns in response to aromatase inhibitor treatment are also discussed.
Tomy
Sherly
S
Department of Aquaculture, National Taiwan Ocean University, Keelung 202, Taiwan.
Wu
Guan-Chung
GC
Huang
Horng-Ru
HR
Chang
Ching-Fong
CF
D000375:Aging / Q000502:physiology*; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007124:Immunoenzyme Techniques / Q000379:methods; D008297:Male; D010473:Perciformes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism*; D012733:Sex Differentiation / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology*; D013739:Testosterone / Q000378:metabolism
D029941:Fish Proteins; D011987:Receptors, Steroid; D013739:Testosterone; D004958:Estradiol; D001141:Aromatase