title: Induction of growth phase-specific autolysis in Bacillus subtilis 168 by growth inhibitors.
abstract: Growth phase-specific autolysis of Bacillus subtilis by inhibitors of membrane permeability, inhibitors of macromolecule biosynthesis, inhibitors of cell wall biosynthesis and detergents were tested and characterized in glucose limited liquid medium. The minimum autolysin induction concentration (MAIC) of test compounds, which was at least 1/20th lower than the conventional autolysis induction concentration, induced autolysis only for cells at the glucose exhaustion point (diauxic point) of the growth phase, while it was not induced for cells at pre- and post-diauxic points. Inhibitors of macromolecule synthesis that are not known for inducing autolysis, such as chloramphenicol, rifampicin, nalidixic acid, and detergents, also induced specific autolysis. Two types of autolysis corresponding to the concentrations of compounds are distinguished: concentration-sensitive and concentration-insensitive types.
authors.affiliation: Department of Physiology, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jin-Kyo
authors.initials: JK
authors.lastname: Chung
authors.forename: Hyun Ee
authors.initials: HE
authors.lastname: Yoon
authors.forename: Ha Chul
authors.initials: HC
authors.lastname: Shin
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Choi
authors.forename: Woo-Hyeon
authors.initials: WH
authors.lastname: Byeon
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D001433:Bacteriolysis / Q000187:drug effects*; D002473:Cell Wall / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D019810:Sodium Azide / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D006131:Growth Inhibitors; D007476:Ionophores; D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D013501:Surface-Active Agents; D003609:Dactinomycin; D012967:Sodium Dodecyl Sulfate; D002701:Chloramphenicol; D000667:Ampicillin; D019810:Sodium Azide; D009238:N-Acetylmuramoyl-L-alanine Amidase; D005947:Glucose; D012293:Rifampin