Effects of bicyclol on liver regeneration after partial hepatectomy in rats.
Bicyclol is a synthetic antihepatitis drug with antioxidative property. The present study was performed to investigate the effect of bicyclol on liver regeneration after partial hepatectomy in rats. Bicyclol (300 mg/kg) was given to rats subjected to 70% hepatectomy three times before operation. At 6, 24, and 48 h after resection, samples were collected for the measurement of serum alanine aminotransferase (ALT), total bilirubin (TBil), hepatic glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH). Moreover, liver regeneration rate, proliferating cell nuclear antigen (PCNA) labeling, proliferation index, and histopathological examination were evaluated at 48 h after hepatectomy. As a result, bicyclol significantly increased regeneration rate, mitotic index (MI), PCNA labeling index, and proliferation index in PH rats. Additionally, bicyclol remarkably inhibited the elevation of serum ALT and TBil levels, alleviated the formation of liver MDA, restored impaired antioxidant SOD and GSH, increased hepatic glycogen content, and also attenuated hepatic vacuolar degeneration. These results suggested that bicyclol had a beneficial effect on liver regenerative capacity of the remnant liver tissue after hepatectomy, probably due to its antioxidative property.
Yao
Xiao-Min
XM
Department of New Drug Development, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1, Xian Nong Tan Street, Beijing, 100050, People's Republic of China. yaoxiaominshanyong@yahoo.com.cn
Zhao
Jing
J
Li
Yue
Y
Li
Yan
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D001713:Biphenyl Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D049109:Cell Proliferation; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008107:Liver Diseases / Q000473:pathology / Q000517:prevention & control; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008940:Mitotic Index; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D001713:Biphenyl Compounds; D018809:Proliferating Cell Nuclear Antigen; D008315:Malondialdehyde; D006003:Glycogen; C477843:bicyclol; D013482:Superoxide Dismutase; D000410:Alanine Transaminase; D005978:Glutathione; D001663:Bilirubin