Comparative methods for the evaluation of protein adsorption.
AFM, Immunogold labeling and SPR have been successfully applied to the analysis of protein adsorption on fibroin substrates, chosen as a paradigmatic biomaterial. The process was evaluated both under static and dynamic conditions, also considering the effect of protein competition. AFM via antibody-modified tips provided maps of protein adsorption and information on protein-protein interactions. Double labeling Immunogold imaged protein distribution upon competitive adsorption. SPR described the dynamics of the process, offering also quantitative data both from individual and two-component protein solutions. The integration of these analytical techniques and the critical evaluation of the results can provide a detailed description of protein adsorption.
Servoli
Eva
E
Department of Materials Engineering and Industrial Technologies and INSTM Research Unit, University of Trento, via Mesiano 77, 38100 Trento, Italy. servolie@ing.unitn.it
Maniglio
Devid
D
Aguilar
Maria Rosa
MR
Motta
Antonella
A
Vazquez
Blanca
B
Roman
Julio San
JS
Migliaresi
Claudio
C
D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005349:Fibroins / Q000737:chemistry*; D007150:Immunohistochemistry / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods
D011506:Proteins; D005349:Fibroins