Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus.
DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.
Tsukuda
Toyoko
T
Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, United States.
Trujillo
Kelly M
KM
Martini
Emmanuelle
E
Osley
Mary Ann
MA
D042002:Chromatin Assembly and Disassembly; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal; D049770:Genes, Mating Type, Fungal; D008954:Models, Biological; D008957:Models, Genetic; D009707:Nucleosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D004271:DNA, Fungal; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins