Hapten synthesis and enzyme-linked immunosorbent assay for phosmet residues: assay optimization and investigation of matrix effects from different food samples.
In this study, a panel of haptens was synthesized for immunoconjugate preparation, and several haptens for heterologous tracer conjugates were also prepared. A highly sensitive polyclonal antibody against the organophosphorus insecticide phosmet was obtained and competitive direct enzyme-linked immunosorbent assays (cd-ELISA) for this pesticide were developed. In the cd-ELISA, the limit of detection (IC(15)) was 0.6 microg kg(-1) and the sensitivity (IC(50)) was 20 microg kg(-1). The suitability of the ELISA for pesticide quantification in peach, apple, orange juice, and apple juice was also studied. Good accuracy and precision were obtained with mean recoveries between 78% and 102.3% and mean coefficients of variation below 13.63%. Validation of the ELISA was conducted by high-performance liquid chromatography. The correlation between the data obtained using the microwell assay and the high-performance liquid chromatography was good (R(2) = 0.9849). The developed immunoassay methods were suitable for the rapid quantitative or qualitative determination of phosmet in food samples.
Song
Yang
Y
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300222, China.
Ge
Yu
Y
Zhang
Yan
Y
Liu
Bing
B
Lu
Yang
Y
Dong
Tingting
T
Wang
Shuo
S
D001628:Beverages / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis / Q000379:methods*; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D027845:Malus; D015394:Molecular Structure; D010573:Pesticide Residues / Q000032:analysis* / Q000276:immunology; D010706:Phosmet / Q000032:analysis* / Q000276:immunology; D027861:Prunus; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006241:Haptens; D007106:Immune Sera; D010573:Pesticide Residues; D010706:Phosmet