Dispersed phantom scatterer technique reveals subtle differences in substrate recognition by phospholipase D inactive mutants.
An elastic light-scattering-based method that makes use of phantom nanoparticles as a substrate organizer (see illustration) allowed the quantitative evaluation of the molecular recognition events between a series of inactivated mutants of phospholipase D and lysophosphatidylcholine. The results highlight the remarkable effects on binding capability caused by single amino acid substitutions.
Morasso
Carlo
C
Istituto Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, Milan, Italy.
Bellini
Tommaso
T
Monti
Daniela
D
Bassi
Mattia
M
Prosperi
Davide
D
Riva
Sergio
S
D000818:Animals; D002417:Cattle; D008027:Light; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D053758:Nanoparticles / Q000737:chemistry; D010739:Phospholipase D / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D012542:Scattering, Radiation; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity
D050505:Mutant Proteins; D010739:Phospholipase D