Fatty acid uptake by breast cancer cells (MDA-MB-231): effects of insulin, leptin, adiponectin, and TNFalpha.
In order to exert metabolic effects, fatty acids must be taken up by cells and metabolize effectively to different classes of cellular lipids (triacylglycerols, phospholipids, etc.) for incorporation into different cellular and intracellular compartments. Therefore, the main aim of the present study is to investigate the uptake and metabolism of fatty acids representing three different series of fatty acids such as oleic acid, 18:1n-9 (OA), arachidonic acid, 20:4n-6 (AA), and eicosapentaneoic acid, 20:5n-3 (EPA) by breast cancer cells, MDA-MB-231. Moreover, we investigated the effects of insulin and several adipokines on the fatty acid uptake by these cells as obesity and insulin resistance syndrome have been suggested to affect breast cancer risk. We report for the first time that AA was predominantly taken up by these cells compared with EPA and OA. Pre-incubation of these cells with TNFalpha stimulated most of the uptake of EPA (30%), whereas uptake of OA and AA was stimulated only 10-15% compared with the controls. Insulin, leptin, and adiponectin had no effect on fatty acid uptake by these cells. Together these results demonstrate that preferential uptake of AA in MDA-MB-231 cells, and the fatty acid uptake activity of these cells is influenced by TNFalpha.
Kaur
Baljit
B
Department of Nutrition, Faculty of Medicine, Institute for Basic Medical Sciences, University of Oslo, PO Box 1046 Blindern, N-0316 Oslo, Norway.
Jørgensen
Aud
A
Duttaroy
Asim K
AK
D052242:Adiponectin / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002788:Cholesterol Esters / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D020738:Leptin / Q000494:pharmacology*; D019301:Oleic Acid / Q000378:metabolism / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D052242:Adiponectin; D002788:Cholesterol Esters; D004075:Diglycerides; D005227:Fatty Acids; D007328:Insulin; D020738:Leptin; D010743:Phospholipids; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D016718:Arachidonic Acid; D019301:Oleic Acid; D015118:Eicosapentaenoic Acid