title: Production of conjugated linoleic acid by probiotic Lactobacillus acidophilus La-5.
abstract: OBJECTIVE
  To study the ability of the probiotic culture Lactobacillus acidophilus La-5 to produce conjugated linoleic acid (CLA), which is a potent anti-carcinogenic agent.
  
  
  RESULTS
  The conversion of linoleic acid to CLA was studied both by fermentation in a synthetic medium and by incubation of washed cells. Accumulation of CLA was monitored by gas chromatography analysis of the biomass and supernatants. While the fermentation conditions applied may not be optimal to observe CLA production in growing La-5 cells, the total CLA surpassed 50% of the original content in the washed cells after 48 h under both aerobic and micro-aerobic conditions. The restriction of oxygen did not increase the yield, but favoured the formation of trans, trans isomers.
  
  
  CONCLUSIONS
  The capability of L. acidophilus La-5 to produce CLA is not dependant on the presence of milk fat or anaerobic conditions. Regulation of CLA production in this strain needs to be further investigated to exploit the CLA potential in fermented foods.
  
  
  CONCLUSIONS
  Knowledge gained through the conditions on the accumulation of CLA would provide further insight into the fermentation of probiotic dairy products. The capacity of the nongrowing cells to produce CLA is also of great relevance for the emerging nonfermented probiotic foods.
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, QC, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Macouzet
authors.forename: B H
authors.initials: BH
authors.lastname: Lee
authors.forename: N
authors.initials: N
authors.lastname: Robert
mesh_terms: D000818:Animals; D018533:Biomass; D002849:Chromatography, Gas; D003470:Culture Media; D005285:Fermentation; D007536:Isomerism; D007779:Lactobacillus acidophilus / Q000254:growth & development / Q000378:metabolism*; D019787:Linoleic Acid / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D008892:Milk / Q000382:microbiology; D019936:Probiotics
chemical_list: D003470:Culture Media; D044243:Linoleic Acids, Conjugated; D019787:Linoleic Acid