One-dimensional topography underlies three-dimensional fibrillar cell migration.
Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (microPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II contractility and microtubules (MTs). 1D and 3D migration are also characterized by an anterior MT bundle with a posterior centrosome. We propose that cells migrate rapidly through 3D fibrillar matrices by a 1D migratory mechanism not mimicked by 2D matrices.
Doyle
Andrew D
AD
Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. adoyle@mail.nih.gov
Wang
Francis W
FW
Matsumoto
Kazue
K
Yamada
Kenneth M
KM
D000818:Animals; D018929:Cell Culture Techniques; D002465:Cell Movement; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D009218:Myosins / Q000378:metabolism; D041681:NIH 3T3 Cells
D009218:Myosins