Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.
Previous studies demonstrated that CD147 molecule, highly expressed on the surface of various malignant tumor cells, significantly correlated with the malignancy of these cancers; however, the role of HAb18G/CD147 in endothelial cells has yet to be established. In this study, we found that the expression of HAb18G/CD147 was significantly upregulated in activated HUVECs. The inhibition of HAb18G/CD147 expression by specific siRNA led to significantly decreased angiogenesis in vitro. Our data indicate that HAb18G/CD147 may regulate angiogenesis via several mechanisms including proliferation, survival, migration, MMPs secretion, and PI3K/Akt activation. Our findings for the first time suggest that upregulation of HAb18G/CD147 in activated HUVECs might play an important role in angiogenesis.
Chen
Yanke
Y
Department of Cell Biology and Cell Engineering Research Center and State Key Laboratory of Cancer Biology, Fourth Military Medical University, 17 West Changle Street, Xi'an 710032, China.
Zhang
Hongxin
H
Gou
Xingchun
X
Horikawa
Yohei
Y
Xing
Jinliang
J
Chen
Zhinan
Z
D015703:Antigens, CD / Q000235:genetics; D051926:Basigin / Q000235:genetics*; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D009369:Neoplasms / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000235:genetics; D018919:Neovascularization, Physiologic / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology*; D015854:Up-Regulation
D015703:Antigens, CD; C497886:BSG protein, human; D034741:RNA, Small Interfering; D051926:Basigin