title: Comparison of two combinations of cyanine dyes for prelabelling and gel electrophoresis.
abstract: We report the use of IC-OSu ethyl-Cy3 and ethyl-Cy5 N-hydroxysuccinimide ester (NHS) cyanine dyes, which have similar chemical properties as the CyDye DIGE fluor minimal dyes for pre-electrophoresis labelling. Multiple sample analyses in different laboratories indicate that the use of IC-OSu ethyl-Cy3 and ethyl-Cy5 NHS ester cyanine dyes produces equivalent results to those obtained with DIGE CyDyes, and allows sample multiplexing and accurate quantitation for differential proteome analysis.
authors.affiliation: Laboratory for Molecular Structure, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, UK.
authors.forename: Nikos
authors.initials: N
authors.lastname: Tsolakos
authors.forename: Tanasit
authors.initials: T
authors.lastname: Techanukul
authors.forename: Amanda
authors.initials: A
authors.lastname: Wallington
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Christopher
authors.initials: C
authors.lastname: Jones
authors.forename: Judit
authors.initials: J
authors.lastname: Nagy
authors.forename: Jun X
authors.initials: JX
authors.lastname: Wheeler
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism*; D002457:Cell Extracts; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D051379:Mice; D011506:Proteins / Q000032:analysis; D012984:Software; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002232:Carbocyanines; D002457:Cell Extracts; D011506:Proteins