[Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis].
OBJECTIVE
To investigate the apoptosis-inducing effect of caspase-3 on human nasopharyngeal carcinoma cells (CNE2).


METHODS
Recombinant caspase-3 was subcloned into the eukaryotic expression vector PEGFP-C1 containing the reporter gene using DNA recombinant technique. CNE2 cells were transfected with the recombinant caspase-3 gene via lipofectamine 2000 and the expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction. The cell morphological changes were observed under fluorescence microscope and electron microscope and the cell survival rate after the transfection was assessed by MTT assay.


RESULTS
Transfection with the recombinant caspase-3 gene induced significant apoptosis in CNE2 cells, which exhibited obvious morphological changes typical of apoptotic cells.


CONCLUSIONS
The recombinant caspase-3 gene can inhibit the growth and effectively induce apoptosis of CNE2 cells.
Wang
Xiao-xia
XX
Department of Otolaryngology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China. xiaoxiawang9139@sina.com
Yao
Xiao-bao
XB
Ji
Xian-sheng
XS
Zhu
Hong-liang
HL
D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D053148:Caspase 3