title: Cr (VI) remediation by indigenous bacteria in soils contaminated by chromium-containing slag.
abstract: Hexavalent chromium (Cr) is a toxic element causing serious environmental threat. Recently, more and more attention is paid to the bio-remediation of Cr (VI) in the contaminated soils. Cr (VI) remediation by indigenous bacteria in soils contaminated by chromium-containing slag at a steel-alloy factory in Hunan Province, China, was investigated in the present study. The results showed that when sufficient nutrients were amended into the contaminated soils, total Cr (VI) concentration declined from the initial value of 462.8 to 10 mg kg(-1) at 10 days and the removal rate was 97.8%. Water soluble Cr (VI) decreased from the initial concentration of 383.8 to 1.7 mg kg(-1). Exchangeable Cr (VI) and carbonates-bound Cr (VI) were removed by 92.6% and 82.4%, respectively. Meanwhile, four Cr (VI) resistant bacterial strains were isolated from the soil under the chromium-containing slag. Only one strain showed a high ability for Cr (VI) reduction in liquid culture. This strain was identified as Pannonibacter phragmitetus sp. by gene sequencing of 16S rRNA. X-ray photoelectron spectroscope (XPS) analysis indicated that Cr (VI) was reduced into trivalent chromium. The results suggest that indigenous bacterial strains have potential application for Cr (VI) remediation in the soils contaminated by chromium-containing slag.
authors.affiliation: School of Metallurgical Science & Engineering, Central South University, Changsha, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Chai
authors.forename: Shunhong
authors.initials: S
authors.lastname: Huang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D002857:Chromium / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D012989:Soil Pollutants; D002857:Chromium; C074702:chromium hexavalent ion