A ratiometric fluorescent sensor with a large Stokes shift for imaging zinc ions in living cells.
The ratiometric fluorescent sensor based on furoquinoline has been designed and synthesized, and not only exhibits a large Stokes shift of 199 nm in a mixture of C(2)H(5)OH-H(2)O (v/v = 8 ratio 2, 10 mM Tris-HCl, pH = 7.3), but can selectively detect zinc ions in the presence of competing metal ions with a red-shift of 75 nm; moreover, cell stain experiments show that can readily reveal a change in intracellular Zn(2+).
Xue
Lin
L
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Liu
Chun
C
Jiang
Hua
H
D000818:Animals; D015374:Biosensing Techniques; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D005663:Furans / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D011725:Pyridines / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
D005456:Fluorescent Dyes; D005663:Furans; D011725:Pyridines; D011804:Quinolines; C037219:quinoline; D015032:Zinc