[The change of certain biochemical indexes and apotosis protein (Fas/FasL) expression in rat liver of selenium and iodine deficiency].
OBJECTIVE
To investigate the effect of selenium (Se) and iodine (I) deficiency on certain biochemical indexes and apotosis protein (Fas/FasL) expression.


METHODS
Healthy SD rats were divided into 4 groups at random, including control group, Se deficient group, I deficient group and both Se and I deficient group.The rats in each group were given different man-made feeds containing different levels of Se and I to breed. The second generation rats were chosen as research subjects. The GPX-Px activity and MDA and NO contents and expression of apoptosis protein (Fas/FasL) in liver were determined by chromometry and western blot.


RESULTS
GPX-Px of rat liver in Se deficient group significantly reduced, MDA and NO content s and expression of apoptosis of Fas/FasL increased. There were no apparent change in GPX-Px activities and NO contents of rat liver in I deficient group, while the contents of MDA and Fas/FasL expressions were much higher. The changes of GSH-Px activities and contents of MDA, NO as well as the Fas/FasL expressions in both Se and I deficiency groups are much higher than those of Selenium and Iodine deficiency groups.


CONCLUSIONS
Se deficiency can cause the decrease of GPX-Px activities of rat liver and the increase of contents of MDA and NO. It can also lead to over expression of Fas/FasL. It seems that I deficiency can enhance the effect of selenium deficiency.
Zhang
Danying
D
Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
Lei
Yanxia
Y
Zhao
Junjie
J
Tan
Wuhong
W
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007455:Iodine / Q000172:deficiency*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000172:deficiency*; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D019014:fas Receptor; D009569:Nitric Oxide; D008315:Malondialdehyde; D007455:Iodine; D005979:Glutathione Peroxidase; D012643:Selenium