Gene transfer and protein release of fission yeast by application of a high voltage electric pulse.
A high voltage electric pulse can be applied to induce the uptake of DNA into cells and the release of protein from cells. In transformation procedures, electroporation is widely used since the technique is simple, rapid, reproducible, and highly efficient. In extraction of protein, on the other hand, electroextraction has many advantages over other conventional extractions. We have developed a highly efficient method for the electroporation of fission yeast. In particular, application of a high voltage electric pulse to fission yeast improves the cellular uptake and release of macromolecules controlled by both osmotic conditions and electric field strength.
Suga
Minoru
M
Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama City, Toyama, 930-8555, Japan. suga@eng.toyama-u.ac.jp
Hatakeyama
Toyomasa
T
D004247:DNA / Q000235:genetics*; D004558:Electric Stimulation; D018014:Gene Transfer Techniques; D011506:Proteins / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*
D011506:Proteins; D004247:DNA