[A method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles].
OBJECTIVE
To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.


METHODS
Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis.


RESULTS
There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05).


CONCLUSIONS
It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.
Feng
Yu
Y
Institute of Forensic Science, Chongqing Public Security Bureau, Chongqing 400042, China. fy777777@126.com
Yan
Mi
M
Liu
Yun
Y
Chen
Jun
J
D004247:DNA / Q000032:analysis*; D004847:Epithelial Cells / Q000378:metabolism*; D053803:Forensic Genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D009061:Mouth Mucosa / Q000166:cytology*; D016133:Polymerase Chain Reaction; D014099:Toothbrushing
D004247:DNA