Practical exercises.
The aim of this chapter is to illustrate the principles of ELISA by (1) showing worked examples of each assay, including diagrams of plates and representational data from assays, (2) analyzing such data in terms of important rules that are learned at each stage, and (3) providing full working instructions for investigators to perform each assay so that they obtain their own data to be analyzed. This chapter can be used in several ways. First, researchers without access to reagents will obtain a working knowledge of ELISA through the examples. Second, it can be used in training courses in which reagents may be provided (as indicated in the text). Third, the information will be useful for investigators who have already had some experience with the technique but may have had difficulties in obtaining and analyzing data. Remember that it is the application of ELISA to specific problems, and not the methodology for its own sake, that is the most important reason the techniques should be mastered.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D035843:Biomedical Research / Q000193:education*; D002417:Cattle / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006168:Guinea Pigs / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D017186:Titrimetry / Q000379:methods
D000906:Antibodies; D000941:Antigens