title: Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions.
abstract: Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Headington, Oxford OX3 7BN, UK.
authors.forename: Max
authors.initials: M
authors.lastname: Crispin
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Bowden
authors.forename: Charlotte H
authors.initials: CH
authors.lastname: Coles
authors.forename: Karl
authors.initials: K
authors.lastname: Harlos
authors.forename: A Radu
authors.initials: AR
authors.lastname: Aricescu
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: E Yvonne
authors.initials: EY
authors.lastname: Jones
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D002240:Carbohydrate Sequence; D018360:Crystallography, X-Ray; D006031:Glycosylation; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055672:Static Electricity
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009844:Oligosaccharides; D011994:Recombinant Proteins; C028569:oligomannoside