title: Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source.
abstract: A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (micro-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol.
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Kangning
authors.initials: K
authors.lastname: Ren
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Liang
authors.forename: Xuan
authors.initials: X
authors.lastname: Mu
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D053801:Capillary Electrochromatography / Q000295:instrumentation*; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008027:Light; D008838:Microcomputers; D036103:Nanotechnology
chemical_list: D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate