title: Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies.
abstract: The human antibody response to polyomavirus capsid proteins is not well characterized. Recombinant BK virus (BKV), JC virus (JCV) and simian virus 40 (SV40) virus-like particles (VLP) were produced in a baculovirus system, and mouse monoclonal antibodies (mAbs) to these proteins were generated using standard methods. Nine of 12 BKV mAbs showed neutralizing activity. The non-neutralizing antibodies also bound BKV pseudocapsids in an ELISA binding assay. Most antibodies recognized conformational species-specific epitopes, but several exceptions were found: (i) BKV mAb BK-F11 cross-reacted with a linear buried epitope common to both JCV and SV40 pseudocapsids, (ii) two of six JCV antibodies (JC-6.7 and JC-7.9) and two of 13 SV40 antibodies (VP1-H2 and VP1-I2) recognized linear buried epitopes common to all three viruses and (iii) SV40 antibody VP1-E5 recognized a linear surface epitope on JCV pseudocapsids.
authors.affiliation: Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
authors.forename: Parmjeet
authors.initials: P
authors.lastname: Randhawa
authors.affiliation: Department of Pediatrics, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Raphael
authors.initials: R
authors.lastname: Viscidi
authors.affiliation: Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Carter
authors.affiliation: Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA.
authors.forename: Denise A
authors.initials: DA
authors.lastname: Galloway
authors.affiliation: Department of Pathology, Jake Gittlen Cancer Research Foundation, Pennsylvania State University, Hershey, USA.
authors.forename: Tim D
authors.initials: TD
authors.lastname: Culp
authors.affiliation: Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
authors.forename: Cathy
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
authors.forename: Bala
authors.initials: B
authors.lastname: Ramaswami
authors.affiliation: Department of Pathology, Jake Gittlen Cancer Research Foundation, Pennsylvania State University, Hershey, USA.
authors.forename: Neil D
authors.initials: ND
authors.lastname: Christensen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001739:BK Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016367:Baculoviridae / Q000378:metabolism; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology*; D003429:Cross Reactions; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes