Proteomic analysis of the secretome of rice calli.
The cell wall and extracellular matrix in higher plants include secreted proteins that play critical roles in a wide range of cellular processes, such as structural integrity and biogenesis. Compared with the intensive cell wall proteomic studies in Arabidopsis, the list of cell wall proteins identified in monocot species is lacking. Therefore, we conducted a large-scale proteomic analysis of secreted proteins from rice. Highly purified secreted rice proteins were obtained from the medium of a suspension of callus culture and were analyzed with multidimensional protein identification technology (MudPIT). As a result, we could detect a total of 555 rice proteins by MudPIT analysis. Based on bioinformatic analyses, 27.7% (154 proteins) of the identified proteins are considered to be secreted proteins because they possess a signal peptide for the secretory pathway. Among the 154 identified proteins, 27% were functionally categorized as stress response proteins, followed by metabolic proteins (26%) and factors involved in protein modification (24%). Comparative analysis of cell wall proteins from Arabidopsis and rice revealed that one third of the secreted rice proteins overlapped with those of Arabidopsis. Furthermore, 25 novel rice-specific secreted proteins were found. This work presents the large scale of the rice secretory proteome from culture medium, which contributes to a deeper understanding of the rice secretome.
Cho
Won Kyong
WK
Division of Applied Life Science (BK21 Program), Graduate School, Environmental Biotechnology National Core Research Center, PMBBRC, Gyeongsang National University, Jinju, Korea.
Chen
Xiong Yan
XY
Chu
Hyosub
H
Rim
Yeonggil
Y
Kim
Suwha
S
Kim
Sun Tae
ST
Kim
Seon-Won
SW
Park
Zee-Yong
ZY
Kim
Jae-Yean
JY
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D019295:Computational Biology; D003470:Culture Media; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
D003470:Culture Media; D010940:Plant Proteins; D020543:Proteome