[Study on the theraputic effect of plants of Camellia genus on osteoporosis].
OBJECTIVE
To observe anti-osteoporotic effect of Plants of Camelia genus induced by retinoic acid in rats, in adqulis crude drug dosage, and to compare activities of them.


METHODS
Extracts of Camellia japonica and Camellia oleifera were given to rats with osteoporosis induced by retinoic acid, some indexes of rats were measured and compared with those of modle group, control group and positive control group, including weight/length (G/L), bone density, earth and calcium content of bone, morphology change and serum calcium, tartrate-resistant acid phosphatase and alkaline phosphatase. We also compared effective intensity between different groups in adqulis crude drug dosage.


RESULTS
Ethanol extracts of seed from Camellia japonica 0.51 g/kg could markedly enhance weight/length (G/L), bone density of femur, serum calcium and alkaline phosphatase level, with the decreasing of anti-tartaric acid tartrate-resistant acid phosphatase level. Meanwhile, they were accompanied by a significant increase of morphologic observed sclerotomal cell and by a significant decrease of osteoclast. Moreover, it was observed greatly that bone trabecula transformateed to normal morphous. The results of this study indicated that effects of ethanol extracts of seed from Camellia japonica on anti-osteoporosis with retinoic acid were the strongest. Ethanol extracts of seed from Camellia japonica , ethanol extracts of leaves from Camellia Oleifera, and aqueous extracts of leaves from Camellia Oleifera were stronger than positive control drug. The other extracts didnt show obvious anti-osteoporotic effects. Eventually the strength order of each group on anti-osteoporosis was as following: ethanol extracts of seed from Camellia japonica > ethanol extracts of leaves from Camellia Oleifera > aqueous extracts of leaves from Camellia Oleifera > aqueous extracts of seed from Camellia Oleifera > positive control drug > aqueous extracts of seed from Camellia Japonica.


CONCLUSIONS
Plants of Camellia genus have different degree anti-osteoporosis effect, which can offer significant theory basis for progressive investigation and exploitation of them.
Tang
Ling
L
College of Bioengineering, Dalian University, Dalian 116622, China. tangyuling6688@163.com
Feng
Bao-Min
BM
Shi
Li-Ying
LY
Wu
Xiao-Juan
XJ
Shi
Hai-Bo
HB
Miao
Yan-Bo
YB
Wang
Yong-Qi
YQ
D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects*; D002118:Calcium / Q000097:blood; D028244:Camellia / Q000737:chemistry* / Q000145:classification; D028241:Camellia sinensis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005269:Femur / Q000187:drug effects; D007527:Isoenzymes / Q000097:blood; D008297:Male; D010024:Osteoporosis / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013662:Tea / Q000737:chemistry; D014212:Tretinoin
D004365:Drugs, Chinese Herbal; D007527:Isoenzymes; D013662:Tea; D014212:Tretinoin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002118:Calcium