[MTT assay for detecting 5-fluorouracil chemosensitivity of human breast carcinoma cell line].
OBJECTIVE
To assess the feasibility of MTT colorimetric assay for testing the in vitro chemosensitivity of breast cancer cells to 5 fluorouracil (5-Fu).


METHODS
The chemosensitivity of human breast carcinoma cell lines MCF-7 and MDA-MB-435S to 5-Fu at different concentrations was evaluated with MTT assay.


RESULTS
5-FU treatment resulted in dose-dependent growth inhibition of the breast cancer cells with both low and high metastatic capacities.


CONCLUSIONS
MTT assay may help select appropriate chemotherapeutic agents and provides evidence for individualized chemotherapy for breast cancer.
Han
Fang-xuan
FX
Department of Pharmacy, People's Hospital of Hainan Province, Haikou 570311, China. hfx1223@163.com
Lin
Hui
H
Ru
Ling
L
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D013778:Tetrazolium Salts
D000964:Antimetabolites, Antineoplastic; D004396:Coloring Agents; D013778:Tetrazolium Salts; D005472:Fluorouracil