[Immunocytochemical demonstration of tissue antigens after a long-term storage of samples in methylsalicylate].
The aim of the present study was to test the possibility of different antigens detection in the biological objects after their storage in methylsalicylate for two or more years before embedding in paraffin, using routine immunocytochemical methods. Using immunocytochemical reactions demostrating intermediate filaments nestin, vimentin, and GFAP, as well as neuronal markers NeuN, neuron-specific enolase, and doublecortin, as the examples, it was shown that long-term storage in methylsalicylate does not prevent immunocytochemical detection of these antigens.
Korzhevskiĭ
D E
DE
Giliarov
A V
AV
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000166:cytology; D003890:Desiccation; D000089662:Doublecortin Protein; D004622:Embryo, Mammalian / Q000737:chemistry; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D016612:Paraffin Embedding / Q000379:methods; D051381:Rats; D012459:Salicylates / Q000737:chemistry*
D000941:Antigens; D015415:Biomarkers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D012459:Salicylates; C033069:methyl salicylate