title: Comparison of the suitability of different hydrolytic strategies to predict aroma potential of different grape varieties.
abstract: Precursor extracts obtained from different grape varieties were submitted to harsh acid hydrolysis (pH 2.5, 100 degrees C, 1 h) and enzymatic hydrolysis (AR2000, pH 5, 40 degrees C, 16 h) and were also added to a synthetic must (200 g L(-1) glucose), which was fermented (yeast strain Stellevin NT 116), to compare the "natural hydrolysis" carried by yeast with alternative "fast" hydrolytic strategies. In all cases, released volatile compounds were extracted by SPE and determined by GC-MS. Leaving aside Muscat, differences between varieties were not relevant, although Grenache and Chardonnay presented some key peculiarities. In general, alcoholic fermentation showed the lowest potential to release volatile compounds from aromatic precursors, whereas enzymatic hydrolysis was the most efficient but also the most different. Practically, this implies that the predictive ability of this hydrolytic strategy is rather poor. In contrast, harsh acid hydrolysis can be considered to much more adequately measure the aroma potential of grapes for winemaking, which suggests that transformations taking place during fermentation include relevant chemical rearrangements in acid media that are better predicted by acid hydrolysis.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, Laboratory for Aroma Analysis and Enology, University of Zaragoza, Zaragoza, Spain. natalia.loscos@unizar.es
authors.forename: Natalia
authors.initials: N
authors.lastname: Loscos
authors.forename: Purificación
authors.initials: P
authors.lastname: Hernández-Orte
authors.forename: Juan
authors.initials: J
authors.lastname: Cacho
authors.forename: Vicente
authors.initials: V
authors.lastname: Ferreira
mesh_terms: D019343:Citric Acid; D005285:Fermentation; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D009812:Odorants / Q000032:analysis*; D011096:Polygalacturonase / Q000378:metabolism; D013045:Species Specificity; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D019343:Citric Acid; D011096:Polygalacturonase