title: Detection of serum gastric cancer-associated MG7-Ag from gastric cancer patients using a sensitive and convenient ELISA method.
abstract: OBJECTIVE
  To seek a high sensitive and convenient method for early diagnosis of gastric cancer by testing MG7-Ag in serum of gastric cancer patients and some other control groups using a convenient ELISA method.
  
  
  METHODS
  The expression of serum MG7-Ag was detected in 116 preoperative gastric cancer patients, 63 postoperative gastric cancer patients, 78 precancerous lesion patients, 50 healthy blood donors and patients of other cancers by a convenient ELISA method. For comparison, serum CEA, CA 50, CA 19-9 and TAG-72 were also detected in preoperative gastric cancer patients. Meanwhile, the expression of MG7-Ag was detected by immunohistochemical analysis in the groups of patients with gastric cancer or precancerous lesion mentioned above.
  
  
  RESULTS
  The positive rate of Mg7-Ag determined by ELISA was 83. 6% of preoperative gastric cancer patients, 54.8% of lung cancer patients, 45.5% of rectal cancer patients, 17.6% of colonic cancer patients, 14.2% of breast cancer patients, 47.6% of postoperative gastric cancer patients, 12.8% of precancerous lesions patients and 0% of healthy blood donors, respectively. The sensitivity of ELISA (83.6%) was found to be similar with that of immunohistochemistry (94%, p > 0. 01), while the false positive rate was lower (12.8% vs. 51.3%). MG7-Ag expression level in gastric cancer was correlated with tumor differentiation (p < 0. 01) and pathological stage (p < 0. 01).
  
  
  CONCLUSIONS
  This ELISA method may be a non-invasive candidate method for screening of large population with high risk of gastric cancer.
authors.affiliation: State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Shannxi, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Jin
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Wensen
authors.initials: W
authors.lastname: Xia
authors.forename: Bei
authors.initials: B
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Wenqi
authors.initials: W
authors.lastname: Du
authors.forename: Kun
authors.initials: K
authors.lastname: Yan
authors.forename: Honghong
authors.initials: H
authors.lastname: Wang
authors.forename: Donghong
authors.initials: D
authors.lastname: Yuan
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hui
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Faming
authors.initials: F
authors.lastname: Zhang
authors.forename: Yunping
authors.initials: Y
authors.lastname: Zhao
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood*
chemical_list: D000951:Antigens, Neoplasm; C508753:MG7 antigen, human