title: L-type calcium channels govern calcium signaling in migrating newborn neurons in the postnatal olfactory bulb.
abstract: Newborn inhibitory neurons migrate into existing neural circuitry in the olfactory bulb throughout the lifetime of adult mammals. While many factors contribute to the maturation of neural circuits, intracellular calcium is believed to play an important role in regulating cell migration and the development of neural systems. However, the factors underlying calcium signaling within newborn neurons in the postnatal olfactory bulb are not well understood. Here, we show that migrating, immature neurons in the olfactory bulb subependymal layer (SEL) undergo spontaneous and depolarization-evoked intracellular calcium transients mediated by high-voltage-activated L-type calcium channels. In contrast to migrating immature neurons in other brain regions, modulation of calcium transients in SEL cells does not alter their rate of migration.
authors.affiliation: Department of Neuroscience, University of California, San Diego School of Medicine, La Jolla, California 92093, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Darcy
authors.forename: Jeffry S
authors.initials: JS
authors.lastname: Isaacson
mesh_terms: D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000502:physiology*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D000089622:Doublecortin Domain Proteins; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D018683:Excitatory Amino Acid Agents / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics; D009553:Nimodipine / Q000494:pharmacology; D009830:Olfactory Bulb / Q000166:cytology* / Q000254:growth & development; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D000089622:Doublecortin Domain Proteins; D018683:Excitatory Amino Acid Agents; D005452:Fluoresceins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; C069306:5-chloromethylfluorescein; D049452:Green Fluorescent Proteins; D004533:Egtazic Acid; D009553:Nimodipine; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C046770:EGTA acetoxymethyl ester