Ecophysiology of "Halarsenatibacter silvermanii" strain SLAS-1T, gen. nov., sp. nov., a facultative chemoautotrophic arsenate respirer from salt-saturated Searles Lake, California.
Searles Lake occupies a closed basin harboring salt-saturated, alkaline brines that have exceptionally high concentrations of arsenic oxyanions. Strain SLAS-1(T) was previously isolated from Searles Lake (R. S. Oremland, T. R. Kulp, J. Switzer Blum, S. E. Hoeft, S. Baesman, L. G. Miller, and J. F. Stolz, Science 308:1305-1308, 2005). We now describe this extremophile with regard to its substrate affinities, its unusual mode of motility, sequenced arrABD gene cluster, cell envelope lipids, and its phylogenetic alignment within the order Halanaerobacteriales, assigning it the name "Halarsenatibacter silvermanii" strain SLAS-1(T). We also report on the substrate dynamics of an anaerobic enrichment culture obtained from Searles Lake that grows under conditions of salt saturation and whose members include a novel sulfate reducer of the order Desulfovibriales, the archaeon Halorhabdus utahensis, as well as a close homolog of strain SLAS-1(T).
Blum
Jodi Switzer
JS
U.S. Geological Survey, MS 480, 345 Middlefield Rd., Menlo Park, CA 94025, USA.
Han
Sukkyun
S
Lanoil
Brian
B
Saltikov
Chad
C
Witte
Brian
B
Tabita
F Robert
FR
Langley
Sean
S
Beveridge
Terry J
TJ
Jahnke
Linda
L
Oremland
Ronald S
RS
D000693:Anaerobiosis; D001149:Arsenates / Q000378:metabolism*; D002140:California; D002462:Cell Membrane / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008124:Locomotion; D008969:Molecular Sequence Data; D005810:Multigene Family; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D001149:Arsenates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C025657:arsenic acid