Prediction of preeclampsia by analysis of cell-free messenger RNA in maternal plasma.
OBJECTIVE
The purpose of this study was to predict the occurrence of preeclampsia in a series of patients at gestational week 15-20 weeks, with the use of a panel of messenger RNA markers.


METHODS
Data from 62 patients with preeclampsia who were asymptomatic at the time of blood testing and 310 control subjects were analyzed. Multivariable analysis was performed with discriminant analysis.


RESULTS
Univariable analysis identified vascular endothelial growth factor receptor 1 as the marker with the highest detection rate; placenta-specific 1 with the lowest. Mean estimated score for preeclampsia was 9.4 for control subjects and 72.5 for subjects who experienced preeclampsia. A receiver operating characteristic curve that was obtained with the estimated score for preeclampsia as a test variable yielded a detection rate of 84% (95% CI, 71.8-91.5) at a 5% false-positive rate with an area under the curve of 0.927 (P < .001). Again, detection rate and score for each patient for classification as preeclamptic correlated with severity.


CONCLUSIONS
A panel of messenger RNA is able to detect subjects who will experience preeclampsia.
Purwosunu
Yuditiya
Y
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Sekizawa
Akihiko
A
Okazaki
Shiho
S
Farina
Antonio
A
Wibowo
Noroyono
N
Nakamura
Masamitsu
M
Rizzo
Nicola
N
Saito
Hiroshi
H
Okai
Takashi
T
D000328:Adult; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011446:Prospective Studies; D012333:RNA, Messenger / Q000097:blood*; D055815:Young Adult
D012333:RNA, Messenger