Optimization of production of caffeine demethylase by Pseudomonas sp. in a bioreactor.
The effect of pH, aeration rate, and agitation rate on specific productivity of caffeine demethylase from Pseudomonas sp. was studied in a bioreactor. Maximum specific productivity of caffeine demethylase of 2,214 U g cell dry weight(-1) h(-1) was obtained at 0.27 vvm, 700 rpm, and pH 7.0. Under these conditions, volumetric oxygen transfer coefficient was 74.2 h(-1), indicating that caffeine demethylase production by Pseudomonas sp. was highly oxygen-dependent. Different metabolite formation at different agitation and aeration rates can be used as a strategy for recovery of pharmaceutically important metabolites from caffeine by manipulation of conditions in a bacterial culture. This is the first report on production of high levels of caffeine demethylase in bioreactors.
Gummadi
Sathyanarayana N
SN
Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, India. gummadi@iitm.ac.in
Dash
Swati Sucharita
SS
Devarai
Santhosh
S
D001426:Bacterial Proteins / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D046508:Culture Techniques / Q000379:methods*; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
D001426:Bacterial Proteins; D019388:Cytochrome P-450 CYP1A2; D010100:Oxygen