title: Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase.
abstract: BACKGROUND
  Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme.
  
  
  METHODS
  Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 microg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px.
  
  
  RESULTS
  Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein.
  
  
  CONCLUSIONS
  The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.
authors.affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland. bronzach@yahoo.com
authors.forename: Bronislaw A
authors.initials: BA
authors.lastname: Zachara
authors.forename: Jolanta
authors.initials: J
authors.lastname: Gromadzinska
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Zbrog
authors.forename: Rafal
authors.initials: R
authors.lastname: Swiech
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wasowicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Twardowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Jablonska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Sobala
mesh_terms: D004311:Double-Blind Method; D004790:Enzyme Induction; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology / Q000628:therapy*; D010919:Placebos; D006435:Renal Dialysis; D012643:Selenium / Q000008:administration & dosage*; D013054:Spectrophotometry, Atomic
chemical_list: D010919:Placebos; D005979:Glutathione Peroxidase; D012643:Selenium