Transcriptome analysis of cadmium response in Ganoderma lucidum.
Ganoderma species are white-rot fungi widespread throughout the world. In this study, a wild isolate of Ganoderma lucidum was first collected and its tolerance was tested in a medium containing 3.0 mM CdCl(2). The cDNA-amplified fragment length polymorphism method was conducted to analyze the transcription profiling of this Ganoderma species in response to Cd treatment. In total, 12 925 transcript-derived fragments (TDFs) were amplified using 256 primer combinations. Forty-nine differentially expressed TDFs were confirmed by DNA dot-blot analysis. Northern blot analysis was used to verify the transcription levels of 34 Cd-inducible TDFs. Sequence analysis indicated that genes involved in reactive oxygen species generation, synthesis of sulfur-containing metabolites, translation machinery, DNA repair, transporting system, proteolysis pathway, mitochondria function, and cell wall biosynthesis were upregulated by Cd treatment. Our results provide a genome-wide transcriptome profiling of Cd response in Ganoderma species.
Chuang
Huey-Wen
HW
Department of BioAgricultural Sciences, National Chiayi University, Chiayi, Taiwan.
Wang
I-Wen
IW
Lin
Shen-Yao
SY
Chang
Yueh-Long
YL
D002104:Cadmium / Q000633:toxicity*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D020940:Reishi / Q000187:drug effects* / Q000502:physiology*; D013312:Stress, Physiological
D002104:Cadmium