In vitro and in vivo studies evaluating recombinant plasmid pCXN2-mIzumo as a potential immunocontraceptive antigen.
OBJECTIVE
Study on feasibility of pCXN2-mIzumo as a potential immunocontraceptive antigen.


METHODS
Two groups of mice received 100 microg/mouse plasmids of pCXN2-mIzumo and pCXN2 respectively. RT-PCR Immunofluorescence assay and ELISA were performed to observe pCXN2-mIzumo expression and antibody response in the inoculated mice. Sperm penetration assay and animal mating were employed to detect differences of in vitro fertilization (IVF) rate and mean litter size between the experimental and control groups.


RESULTS
Izumo cDNA positive bands were detected in sample from mice immunized with pCXN2-mIzumo. IgG response started to rise at 2 weeks after first boost and reached the highest antibody titers at 2 weeks after third boost of immunization with pCXN2-mIzumo in the experimental mice. In vitro fertilization rate in the experimental group (11.57%) was significantly lower than that in control (36.60%). Significant difference of mean litter size between female experimental and control groups was observed, and there was significant negative correlation between individual anti-serum titers and litter size (r = -0.308, P < 0.05).


CONCLUSIONS
pCXN2-mIzumo plasmid possesses appreciable anti-fertility potential.
An
Gang
G
Research Center for Reproductive Medicine, Shantou University Medical College, Shantou, Guangdong, China.
Huang
Tian-Hua
TH
Wang
De-Gang
DG
Xie
Qing-Dong
QD
Ma
Lian
L
Chen
De-Yu
DY
D000818:Animals; D000917:Antibody Formation / Q000235:genetics / Q000276:immunology; D003269:Contraception, Immunologic; D005260:Female; D005298:Fertility / Q000235:genetics / Q000276:immunology; D005307:Fertilization in Vitro; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D008098:Litter Size / Q000235:genetics / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009132:Muscles / Q000276:immunology / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000276:immunology*; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology*; D022642:Vaccines, Contraceptive / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
D007136:Immunoglobulins; C498177:Izumo1 protein, mouse; D008565:Membrane Proteins; D022642:Vaccines, Contraceptive; D019444:Vaccines, DNA