title: Induced sputum metalloproteinases and their inhibitors in relation to exhaled nitrogen oxide and sputum nitric oxides and other inflammatory cytokines in patients with chronic obstructive pulmonary disease.
abstract: The aim of this study was to assess sputum levels of the metalloproteinases MMP-1, MMP-2, MMP-3, TIMP-1, and TIMP-2, as well as MMPs/TIMPs ratios in relation to exhaled NO (eNO) and sputum NOs (nitrates and nitrites) and IL8 obtained from chronic obstructive pulmonery disease (COPD) patients, healthy non-smokers, and healthy smokers. We found higher levels of TIMP-1 (118.9 ng/ml) and TIMP-2 (3.75 ng/ml) in COPD patients than in healthy smokers (17.7 ng/ml, P<0.03; 0.51 ng/ml, P>0.05, respectively) and healthy non-smokers (84.6 ng/ml, P>0.05; 1.61 ng/ml, P>0.05, respectively). We also observed significant positive correlations between concentrations of NOs and MMP-1, MMP-2, MMP-3, and TIMP-2 (r=0.37, P<0.02; r=0.60, P<0.0001; r=0.56, P<0.0004 and r=0.47 P<0.004, respectively) in COPD patients. IL8, MMP-2, MMP-3, and TIMP-2 levels in induced sputum were negatively correlated with airway obstruction, i.e., FEV(1)/FVC (r=-0.61, P<0.00009; r=-0.41, P<0.01; r=-0.38, P<0.02; r=-0.49, P<0.002). Our study points to a potentially pathogenic role of stromelysin-1 (MMP-3) in COPD.
authors.affiliation: Department of Lung Diseases and Tuberculosis, Medical University of Silesia, Zabrze, Poland. ftpulmza@sum.edu.pl
authors.forename: D
authors.initials: D
authors.lastname: Ziora
authors.forename: S
authors.initials: S
authors.lastname: Dworniczak
authors.forename: J
authors.initials: J
authors.lastname: Kozielski
mesh_terms: D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D005110:Extracellular Space / Q000201:enzymology / Q000378:metabolism; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism / Q000502:physiology; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D012129:Respiratory Function Tests; D012907:Smoking / Q000378:metabolism; D013147:Spirometry; D013183:Sputum / Q000201:enzymology*
chemical_list: D016207:Cytokines; D009589:Nitrogen Oxides; D011480:Protease Inhibitors; D009569:Nitric Oxide; D020782:Matrix Metalloproteinases; D019278:Matrix Metalloproteinase 3