The Escherichia coli common pilus and the bundle-forming pilus act in concert during the formation of localized adherence by enteropathogenic E. coli.
Although the bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) mediates microcolony formation on epithelial cells, the adherence of BFP-deficient mutants is significantly abrogated, but the mutants are still adherent due to the presence of intimin and possibly other adhesins. In this study we investigated the contribution of the recently described E. coli common pilus (ECP) to the overall adherence properties of EPEC. We found that ECP and BFP structures can be simultaneously observed in the course (between zero time and 7 h during infection) of formation of localized adherence on cultured epithelial cells. These two pilus types colocalized at different levels of the microcolony topology, tethering the adhering bacteria. No evidence of BFP disappearance was found after prolonged infection. When expressed from a plasmid present in nonadherent E. coli HB101, ECP rendered this organism highly adherent at levels comparable to those of HB101 expressing the BFP. Purified ECP bound in a dose-dependent manner to epithelial cells, and the binding was blocked with anti-ECP antibodies, confirming that the pili possess adhesin properties. An ECP mutant showed only a modest reduction in adherence to cultured cells due to background expression levels of BFP and intimin. However, isogenic mutants not expressing EspA or BFP were significantly less adherent when the ecpA gene was also deleted. Furthermore, a DeltaespA DeltaecpA double mutant (unable to translocate Tir and to establish intimate adhesion) was at least 10-fold less adherent than the DeltaespA and DeltaecpA single mutants, even in the presence of BFP. A Delta bfp DeltaespA DeltaecpA triple mutant showed the least adherence compared to the wild type and all the isogenic mutant strains tested, suggesting that ECP plays a synergistic role in adherence. Our data indicate that ECP is an accessory factor that, in association with BFP and other adhesins, contributes to the multifactorial complex interaction of EPEC with host epithelial cells.
Saldaña
Zeus
Z
Department of Immunobiology, University of Arizona, 1501 N. Campbell Ave., Tucson, Arizona 85724, USA.
Erdem
Aysen L
AL
Schüller
Stephanie
S
Okeke
Iruka N
IN
Lucas
Mark
M
Sivananthan
Arunon
A
Phillips
Alan D
AD
Kaper
James B
JB
Puente
José L
JL
Girón
Jorge A
JA
D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission
D029968:Escherichia coli Proteins; C102051:EspA protein, E coli; D036781:Fimbriae Proteins