Inhibition of all-trans retinoic acid-induced granulocytic differentiation of WEHI-3B D+ cells by forced expression of SCL (TAL1) and GATA-1.
All-trans retinoic acid (ATRA) induces granulocytic maturation of WEHI-3B D+ leukemia cells and LiCl enhances this maturation, while WEHI-3B D- cells are non-responsive to ATRA. Transfection of SCL, expressed in D- but absent in D+ cells, into D+ cells, caused resistance to ATRA, while transfection of GATA-1 into D+ cells produced resistance to the combination of ATRA and LiCl. SCL expression in D+ cells did not induce the expression of c-Kit, a putative target gene for SCL. LiCl, known to inhibit some kinases by displacing Mg2+, did not affect tyrosine kinase activity of the cytoplasmic domain of c-Kit.
Ishiguro
Kimiko
K
Department of Pharmacology and Developmental Therapeutics Program, Cancer Center, Yale University School of Medicine, New Haven, CT 06520, USA.
Rice
Anna M
AM
Rice
Kevin P
KP
Sartorelli
Alan C
AC
D000818:Animals; D001483:Base Sequence; D001549:Benzamides; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D017931:DNA Primers; D050982:GATA1 Transcription Factor / Q000378:metabolism*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects*; D000068877:Imatinib Mesylate; D018021:Lithium Chloride / Q000494:pharmacology; D051379:Mice; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D010957:Plasmids; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*
D001549:Benzamides; D017931:DNA Primers; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D015514:Oncogene Proteins, Fusion; D010879:Piperazines; D011743:Pyrimidines; D012333:RNA, Messenger; C103486:SIL protein, mouse; D014212:Tretinoin; D000068877:Imatinib Mesylate; D011505:Protein-Tyrosine Kinases; D018021:Lithium Chloride