[Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle].
OBJECTIVE
To observe the effects of Ganoderma lucidum Spores (GLS) on proliferation and growth cycle in the human hepatoma cell line (HepG2 cells), and study its possible mechanism of activities.


METHODS
The growth inhibition of GLS on HepG2 cells was detected by MTT assay. The DNA contents and the distribution of cell cycle were analyzed by flow cytometry.


RESULTS
The results of MTT assays showed that GLS could inhibit the HepG2 cells growth at a dose and time-dependent manner directly; the inhibition rate of GLS (2500 microg/ml) on HepG2 cells after 72 h was a maximum up 51.4%. The results of flow cytometry experiments showed that GLS (3 mg/ml) could reduce the G2 phase of HepG2 cells and a clear apoptosis peak would be observed when GLS was 6 mg/ml.


CONCLUSIONS
GLS has a direct inhibitory effect on tumor cell proliferation and its growth cycle, it can reduce the G2 phase; and high doses of GLS can also make tumor cells apoptosised.
Li
Lin
L
Department of Pharmacology, School of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China. li75lin@126.com
Li
Ting
T
Wang
Xiao-Jing
XJ
Xu
Jiang-Ping
JP
Wang
Shun-Guan
SG
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D020940:Reishi; D013172:Spores, Fungal; D013997:Time Factors
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal