Colloidal nanogold-based immunochromatographic strip test for the detection of digoxin toxicity.
Digoxin is widely used as a cardiac glycoside drug in the treatment of various heart conditions. Because it is a toxic drug, it should be regularly monitored in the serum of patients under treatment. In this study, colloidal nanogold is synthesized and the preparation of nanogold-labeled monoclonal antibody probe to digoxin is described under optimal conditions. In addition, an immunochromatographic (IC) method for digoxin analysis employing nanogold-labeled probe is developed. With this technique, it requires only 5 min to complete the quantitative detection of digoxin. The detection time is decreased 20-30 times in comparison to radioimmunoassay (RIA). The sensitivity to digoxin was about 2 ng/ml by naked eye, which is within the therapeutic and toxic ranges of digoxin. The results of serum samples obtained by IC strip were in agreement with those obtained by RIA. The IC strip was sufficiently sensitive and accurate to be used for the rapid detection of digoxin in serum samples.
Omidfar
Kobra
K
Laboratory of Biochemistry, Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, Iran. omidfar@tums.ac.ir
Kia
Solmaz
S
Kashanian
Soheila
S
Paknejad
Malihe
M
Besharatie
Afsaneh
A
Kashanian
Susan
S
Larijani
Bagher
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D002845:Chromatography; D003102:Colloids; D004077:Digoxin / Q000032:analysis / Q000276:immunology / Q000378:metabolism / Q000633:toxicity*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011863:Radioimmunoassay; D011934:Reagent Strips; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors
D000911:Antibodies, Monoclonal; D003102:Colloids; D011934:Reagent Strips; D012710:Serum Albumin, Bovine; D004077:Digoxin; D006046:Gold