title: Global proteomic analysis of the chromate response in Arthrobacter sp. strain FB24.
abstract: A global proteomic evaluation of the response of Arthrobacter sp. strain FB24 to 5 and 20 mM Cr(VI) was conducted using both two-dimensional gel electrophoresis (2-DGE) and liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS). The changes in protein expression found with 2-DGE indicate alterations in central metabolism and amino acid synthesis. Proteome coverage increased from 22% with 2-DGE to 71% with LC/LC-MS/MS. The proteins exhibiting the highest levels of expression under Cr(VI) stress suggest intracellular sulfur limitation, which could be driven by competition for the sulfate (SO4(2-)) transporter by the chromate (CrO4(2-)) ion. These results are consistent with the growth defects seen with strain FB24 when Cr(VI) concentrations exceeded 5 mM.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA. khenne@purdue.edu
authors.forename: Kristene L
authors.initials: KL
authors.lastname: Henne
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Turse
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.forename: Mary S
authors.initials: MS
authors.lastname: Lipton
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Tollaksen
authors.forename: Carl
authors.initials: C
authors.lastname: Lindberg
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Babnigg
authors.forename: Carol S
authors.initials: CS
authors.lastname: Giometti
authors.forename: Cindy H
authors.initials: CH
authors.lastname: Nakatsu
authors.forename: Dorothea K
authors.initials: DK
authors.lastname: Thompson
authors.forename: Allan E
authors.initials: AE
authors.lastname: Konopka
mesh_terms: D001173:Arthrobacter / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002840:Chromates / Q000494:pharmacology*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D002840:Chromates; D020543:Proteome