Cephalometric changes produced by locally applied anabolic steroid in Wistar rats.
OBJECTIVE
There is a strong relationship between neuromuscular activity and facial skeletal morphogenesis. The systemic use of anabolic steroid accelerates craniofacial growth and induces high functional activity of rats' masticatory muscles. The present study examines the effect of an anabolic steroid locally applied in the masseter muscle of growing rats with the purpose of verifying possible craniofacial changes with minimum drug side effects, using cephalometric analysis.


METHODS
Forty-three 3-week-old Wistar rats were evaluated and divided into two groups: (1) NAN group (local bilateral masseter injection of nandrolone decanoate - 0.05 microg/3 microl/bi-weekly) and (2) CON group (local bilateral masseter injection of sterile oil - 3 microl). Tukey and Wilcoxon tests were used.


RESULTS
In NAN group the cephalometric measures showed horizontalized facial growth (reduce of FMA angle -p<0.01), increase of posterior facial height, decrease of anterior facial height, reduction of gonial angle (p<0.05), increase of mandibular height (p<0.01). There was no variation in the length of the mandible, in the skull and animals' weight gain, thus demonstrating that no important systemic effect occurred.


CONCLUSIONS
The local injection of nandrolone in the masseter muscle of rats was capable to alter the growth direction and the morphology of the craniofacial complex in Wistar rats.
da Silva
Helena Cristina Francisco Pereira
HC
College and Post Graduate Center of Dentistry, São Leopoldo Mandic, Campinas, Brazil. helenacfps@uol.com.br
Cecanho
Rodrigo
R
D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D002508:Cephalometry; D005147:Facial Bones / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D008406:Masseter Muscle / Q000187:drug effects*; D009277:Nandrolone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000254:growth & development
D045930:Anabolic Agents; D009277:Nandrolone