title: [Studies on extraction of active fraction from Rhizoma et Radix Ligustici by supercritical CO2 extraction].
abstract: OBJECTIVE
  To study the conditions of extraction of active fraction in Rhizoma et Radix Ligustici by supercritical CO2 extraction (SFE).
  
  
  METHODS
  Using the content of ferulic acid as the index, conditions for the extraction including extracting pressure, extracting temperature, temperature of resolution and extracting time were optimized by uniform design.
  
  
  RESULTS
  The best SFE conclusions were as follows: cosolvent was 50% ethanol (ml/g), extracting pressure was 40 MPa, extracting temperature was 50 degrees C, pressure of resolution was 6 MPa, temperature of resolution was 50 degrees C, extracting time was 3.5 hours.
  
  
  CONCLUSIONS
  The optimum extraction has high extraction ratio of active fraction in Rhizoma et Radix Ligustici, so it is reasonable and practicable.
authors.affiliation: Hainan Medical College, Haikou 571101, China.
authors.forename: Cai-Ni
authors.initials: CN
authors.lastname: Mao
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Wan-Rui
authors.initials: WR
authors.lastname: Yuan
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis* / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D009822:Oils, Volatile / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D003373:Coumaric Acids; D009822:Oils, Volatile; D002245:Carbon Dioxide; D000431:Ethanol; C004999:ferulic acid