Microbial degradation of cyclic peptides produced by bacteria.
Bacterial strain, B-9, isolated from Lake Tsukui, Japan, and characterized as genus Sphingosinicella sp., possesses hydrolytic enzymes capable of degrading various toxic and non-toxic cyanobacterial cyclic peptides, such as microcystins, nodularin, microviridin, microcyclamide and aeruginopeptin. In this study, the degradation activities of the cell extract of B-9 against bacterial cyclic peptides, bacitracin, colistin, polymyxin, mikamycin, thiopeptin and WAP-8294A2, were investigated and the degradation products were analyzed using HPLC and liquid chromatography/ion trap tandem mass spectrometry (LC/ITMS). As a result of extensive experiments, it was confirmed that B-9 could also degrade these bacterial cyclic peptides by hydrolysis of their peptide or ester bonds, except for WAP-8294A2. These results indicated that the functions of the bacterium with its enzymes were further extended and offered the possibility of degrading other types of compounds.
Kato
Hajime
H
Graduate School of Environmental and Human Science and Faculty of Pharmacy, Meijo University, Tempaku, Nagoya, Japan.
Tsuji
Kiyomi
K
Harada
Ken-ichi
K
D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007202:Indicators and Reagents; D052998:Microcystins / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism*; D020560:Proteobacteria / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D007202:Indicators and Reagents; D052998:Microcystins; D010456:Peptides, Cyclic