title: Fibrin sealants from fresh or fresh/frozen plasma as scaffolds for in vitro articular cartilage regeneration.
abstract: Our objective was to evaluate human CryoSeal fibrin glue derived from single units of plasma as scaffolds for articular cartilage tissue engineering. Human articular chondrocytes were encapsulated into genipin cross-linked fibrin glue derived from individual units of fresh or frozen plasma using the CryoSeal fibrin sealant (FS) system. The constructs were cultured for up to 7 weeks in vitro under low (5%) or normal (21%) oxygen. Chondrocyte viability was >90% within the fibrin gels. Hypoxia induced significant increases in collagen II and Sox9 gene expression and a significant decrease in collagen I. A significant increase in collagen II was detected in fresh plasma-derived cultures, while only collagen I was significantly increased in frozen plasma cultures. Significant increases in total glycosaminoglycan and collagen were detected in the extracellular matrix secreted by the encapsulated chondrocytes. A significant increase in compression modulus was only observed for fresh plasma-derived gels, which is likely explained by a greater amount of collagen type I detected after 7 weeks in frozen compared to fresh plasma gels. Our results indicate that CryoSeal fibrin glue derived from fresh plasma is suitable as a tissue engineering scaffold for human articular chondrocytes, and therefore should be evaluated for autologous articular cartilage regeneration.
authors.affiliation: Department of Cellular and Molecular Medicine, University of Ottawa , Ottawa, Ontario, Canada.
authors.forename: Emma V
authors.initials: EV
authors.lastname: Dare
authors.forename: May
authors.initials: M
authors.lastname: Griffith
authors.forename: Philippe
authors.initials: P
authors.lastname: Poitras
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Geoffrey F
authors.initials: GF
authors.lastname: Dervin
authors.forename: Antonio
authors.initials: A
authors.lastname: Giulivi
authors.forename: Maxwell T
authors.initials: MT
authors.lastname: Hincke
mesh_terms: D000328:Adult; D001696:Biomechanical Phenomena / Q000187:drug effects; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000187:drug effects* / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism*; D012038:Regeneration / Q000187:drug effects*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015718:Fibrin Tissue Adhesive; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D016023:Integrins; D010100:Oxygen