title: Evaluation of mitochondrial respiratory chain activity in muscle healing by low-level laser therapy.
abstract: BACKGROUND
  Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process.
  
  
  OBJECTIVE
  In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after traumatic muscular injury.
  
  
  METHODS
  Male Wistar rats were randomly divided into three groups (n=6): sham (uninjured muscle), muscle injury without treatment, muscle injury with LLLT (AsGa) 5J/cm(2). Gastrocnemius injury was induced by a single blunt-impact trauma. LLLT was used 2, 12, 24, 48, 72, 96, and 120 hours after muscle-trauma.
  
  
  RESULTS
  Our results showed that the activities of complex II and succinate dehydrogenase after 5days of muscular lesion were significantly increased when compared to the control group. Moreover, our results showed that LLLT significantly increased the activities of complexes I, II, III, IV and succinate dehydrogenase, when compared to the group of injured muscle without treatment.
  
  
  CONCLUSIONS
  These results suggest that the treatment with low-level laser may induce an increase in ATP synthesis, and that this may accelerate the muscle healing process.
authors.affiliation: Laboratório de Fisiologia e Bioquímica do Exercício, Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil. silveira_paulo2004@yahoo.com.br
authors.forename: Paulo C L
authors.initials: PC
authors.lastname: Silveira
authors.forename: Luciano Acordi da
authors.initials: LA
authors.lastname: Silva
authors.forename: Daiane B
authors.initials: DB
authors.lastname: Fraga
authors.forename: Tiago P
authors.initials: TP
authors.lastname: Freitas
authors.forename: Emilio L
authors.initials: EL
authors.lastname: Streck
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pinho
mesh_terms: D000818:Animals; D004579:Electron Transport / Q000502:physiology / Q000528:radiation effects; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D042963:Electron Transport Complex II / Q000528:radiation effects; D003576:Electron Transport Complex IV / Q000528:radiation effects; D028022:Low-Level Light Therapy; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism / Q000528:radiation effects*; D018482:Muscle, Skeletal / Q000293:injuries*; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism / Q000528:radiation effects; D014945:Wound Healing / Q000502:physiology* / Q000528:radiation effects
chemical_list: D045222:Electron Transport Chain Complex Proteins; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV