Pyloric neuron morphology in the stomatogastric ganglion of the lobster, Panulirus interruptus.
The pyloric network of decapod crustaceans has been intensively studied electrophysiologically in the infraorders Astacidea, Brachyura, and Palinura. The morphology of some or all pyloric neurons has been well described in Astacidea and Brachyura, but less so in Palinura. Given the large evolutionary distance between these three groups, and the large amount of electrophysiology that has been performed in palinuroid species, it is important to fill this gap. We describe here the gross morphology of all six pyloric neuron types in a palinuroid, P. interruptus. All pyloric neurons had complicated, extended dendritic trees that filled the majority of the neuropil, with most small diameter processes present in a shell near the surface of the ganglion. Certain neuron types showed modest preferences for somata location in the ganglion, but these differences were too weak to use as identifying characteristics. Quantitative measurements of secondary branch number, maximum branch order, total process length, and neuron somata diameter were also, in general, insufficient to distinguish among the neurons, although AB and LP neuron somata diameters differed from those of the other types. One neuron type (VD) had a distinctive neurite branching pattern consisting of a small initial branch followed shortly by a bifurcation of the main neurite. The processes arising from these two branches occupied largely non-overlapping neuropil. Electrophysiological recordings showed that each major branch had its own spike initiation zone and that, although the zones fired correlated spikes, they generated spikes independently. VD neurons in the other infraorders have similar morphologies, suggesting that having two arbors is important for the function of this neuron. These data are similar to those previously obtained in Brachyura and Astacidea. It thus appears that, despite their long evolutionary separation, neuron morphology in these three infraorders has not greatly diverged.
Thuma
Jeffrey B
JB
Department of Biological Sciences, Ohio University, Athens, Ohio, USA. thuma@ohio.edu
White
William E
WE
Hobbs
Kevin H
KH
Hooper
Scott L
SL
D000200:Action Potentials; D000704:Analysis of Variance; D000818:Animals; D017615:Enteric Nervous System / Q000166:cytology / Q000502:physiology; D017952:Ganglia, Invertebrate / Q000166:cytology* / Q000502:physiology; D008839:Microelectrodes; D008121:Nephropidae / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010802:Phylogeny