title: Expressions of glucose transporter Types 1 and 3 and hexokinase-II in diffuse large B-cell lymphoma and other B-cell non-Hodgkin's lymphomas.
abstract: BACKGROUND
  Diffuse large B-cell lymphoma (DLBCL) has been reported to show higher uptake of 2-deoxy-2-F18-fluoro-d-glucose (FDG) by positron emission tomography than other B-cell non-Hodgkin's lymphomas (non-DLBCL). The authors addressed the mechanism of FDG uptake in DLBCL by immunostaining for glucose transporter Types 1 (Glut-1) and 3 (Glut-3) and hexokinase-II (HK-II) in excised lymphoma tissues.
  
  
  METHODS
  Sixteen B-cell non-Hodgkin's lymphoma patients (11 DLBCL and 5 non-DLBCL patients) were included in the study because the lymphoma tissues obtained by excision were eligible for immunostaining. The expressions of Glut-1, Glut-3 and HK-II were assessed regarding the percentages of positively stained lymphoma cells (%expression), the staining intensities (none=0, weak=1, moderate=2 and strong=3) and the staining patterns (membranous or cytoplasmic) and compared between DLBCL and non-DLBCL.
  
  
  RESULTS
  Glut-1 was not expressed at all in DLBCL or non-DLBCL, and their Glut-3 expressions were not significantly different (P>.05) with respect to %expression (mean+/-S.E.M., 73.6+/-7.3% vs. 72.0+/-3.7%), staining intensity (2.5+/-0.2 vs. 2.6+/-0.2) and staining pattern (membranous pattern dominant; 54.5% vs. 60.0%). However, DLBCL expressed more HK-II than non-DLBCL, i.e., %expression (45.2+/-11.5% vs. 17.0+/-15.8%, P=.0275) and staining intensity (2.3+/-0.2 vs. 0.6+/-0.4, P=.0032). HK-II showed a cytoplasmic location in DLBCL and non-DLBCL.
  
  
  CONCLUSIONS
  HK-II and Glut-3 contribute significantly to FDG uptake in DLBCL. DLBCL may have higher FDG uptake because it expresses more HK-II, whereas Glut-1 appears to play no role in FDG uptake in B-cell non-Hodgkin's lymphoma.
authors.affiliation: Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Korea.
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Shim
authors.forename: Won Woo
authors.initials: WW
authors.lastname: Lee
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Haeryoung
authors.initials: H
authors.lastname: Kim
authors.forename: Young
authors.initials: Y
authors.lastname: So
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Kim
mesh_terms: D000368:Aged; D005260:Female; D019788:Fluorodeoxyglucose F18; D051272:Glucose Transporter Type 1 / Q000032:analysis*; D051274:Glucose Transporter Type 3 / Q000032:analysis*; D006593:Hexokinase / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; D019788:Fluorodeoxyglucose F18; D006593:Hexokinase