Extending the range of microsecond-to-millisecond chemical exchange detected in labeled and unlabeled nucleic acids by selective carbon R(1rho) NMR spectroscopy.
We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.
Hansen
Alexandar L
AL
Department of Chemistry and Biophysics, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA.
Nikolova
Evgenia N
EN
Casiano-Negroni
Anette
A
Al-Hashimi
Hashim M
HM
D002247:Carbon Isotopes; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000737:chemistry
D002247:Carbon Isotopes; D009696:Nucleic Acids; D012335:RNA, Ribosomal; D004247:DNA