Highly sensitive determination of microRNA using target-primed and branched rolling-circle amplification.
One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined.
Cheng
Yongqiang
Y
Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, China.
Zhang
Xian
X
Li
Zhengping
Z
Jiao
Xiaoxia
X
Wang
Yucong
Y
Zhang
Yali
Y
D005453:Fluorescence; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D011130:RNA Ligase (ATP) / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000737:chemistry*
D035683:MicroRNAs; D014764:Viral Proteins; D011130:RNA Ligase (ATP); C467724:bacteriophage T4 RNA ligase 2