DNA damage response of A549 cells treated with particulate matter (PM10) of urban air pollutants.
We describe the events triggered by a sub-lethal concentration of airborne particulate matter (PM(10)) in A549 cells, which include the formation DNA double-strand breaks, gammaH2A.X generation, and 53BP1 recruitment. To protect the genome, cells activated ATM/ATR/Chk1/Chk2/p53 pathway but, after 48 h, cells turned into a senescence-like state. Trolox, an antioxidant, was able to prevent most of the alterations observed after particulate matter exposure, demonstrating the important role of ROS as mediator of PM(10)-induced genotoxicity and suggesting that DNA damage could be the mechanisms by which particulate matter augment the risk of lung cancer.
Sánchez-Pérez
Yesennia
Y
Instituto Nacional de Cancerología (INCan), Subdirección de Investigación Básica, San Fernando No. 22, Tlalpan, 14080 Mexico, DF, Mexico; Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud (DCBS), Universidad Autónoma Metropolitana-Iztapalapa (UAM-Iztapalapa), 09340 Mexico, DF, Mexico.
Chirino
Yolanda I
YI
Instituto Nacional de Cancerología (INCan), Subdirección de Investigación Básica, San Fernando No. 22, Tlalpan, 14080 Mexico, DF, Mexico.
Osornio-Vargas
Álvaro R
ÁR
Instituto Nacional de Cancerología (INCan), Subdirección de Investigación Básica, San Fernando No. 22, Tlalpan, 14080 Mexico, DF, Mexico.
Morales-Bárcenas
Rocío
R
Instituto Nacional de Cancerología (INCan), Subdirección de Investigación Básica, San Fernando No. 22, Tlalpan, 14080 Mexico, DF, Mexico.
Gutiérrez-Ruíz
Concepción
C
Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud (DCBS), Universidad Autónoma Metropolitana-Iztapalapa (UAM-Iztapalapa), 09340 Mexico, DF, Mexico.
Vázquez-López
Inés
I
Instituto Nacional de Cancerología (INCan), Subdirección de Investigación Básica, San Fernando No. 22, Tlalpan, 14080 Mexico, DF, Mexico.
García-Cuellar
Claudia M
CM
Instituto Nacional de Cancerología (INCan), Subdirección de Investigación Básica, San Fernando No. 22, Tlalpan, 14080 Mexico, DF, Mexico. Electronic address: cmgarcia@salud.gob.mx.
D017209:Apoptosis; D064007:Ataxia Telangiectasia Mutated Proteins; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis; D045744:Cell Line, Tumor; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000032:analysis; D006801:Humans; D052638:Particulate Matter / Q000633:toxicity*; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000032:analysis; D020687:cdc25 Phosphatases / Q000032:analysis
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D052638:Particulate Matter; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; C576597:ATR protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases; C517004:CDC25A protein, human; D020687:cdc25 Phosphatases