The flavonoid rutin but not the alkaloid arborinine induces apoptosis in a B-cell hybridoma cell line.
The effects of arborinine, an alkaloid extracted from Erthela bahiensis and of rutin, a flavonoid obtained from Dimorphandra mollis (Benth.), Brazilian medicinal plants, on the viability and function of a murine B-cell hybridoma as a tumor model were investigated. The flavonoid rutin at 50 microM induced an increase in the number of apoptotic cells of one- to fivefold and reductions in cellular proliferation and monoclonal antibody production. Less but still significant necrosis was also induced by rutin under the same experimental conditions. On the other hand, the alkaloid arborinine exerted no significant effects on the studied parameters.
Roseghini
Renata
R
Immunology and Molecular Laboratory, Federal University of Bahia, BA, Brazil. bino7@terra.com.br
Falcão
Georgenes Morato
GM
Oliveira Costa
José Fernando
JF
Clarêncio
Jorge
J
Nascimento
Ivana
I
Velozo
Eudes
E
Schaer
Robert
R
Vale
Vera
V
Costa
Silvia Lima
SL
Costa
Maria de Fátima Dias
Mde F
Tardy
Marcienne
M
Meyer
Roberto
R
Menezes Freire
Songelí
S
D000166:Acridines / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006825:Hybridomas / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009336:Necrosis / Q000139:chemically induced; D010936:Plant Extracts / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*; D012431:Rutin / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds
D000166:Acridines; D000911:Antibodies, Monoclonal; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C022784:arborinine; D012431:Rutin