title: Efficient production of androgenetic embryos by round spermatid injection.
abstract: Mammalian androgenetic embryos can be produced by pronuclear exchange of fertilized oocytes or by dispermic in vitro fertilization of enucleated oocytes. Here, we report a new technique for producing mouse androgenetic embryos by injection of two round spermatid nuclei into oocytes, followed by female chromosome removal. We found that injection of round spermatids resulted in high rates of oocyte survival (88%). Androgenetic embryos thus produced developed into mid-gestation fetuses at various rates, depending on the mouse strain used. All the fetuses examined maintained paternally specific genomic imprinting memories. This technique also enabled us to produce complete heterozygous F1 embryos by injecting two spermatids from different strains. The best rate of fetal survival (12% per embryos transferred) was obtained with C57BL/6 x DBA/2 androgenetic embryos. We also generated embryonic stem cell lines efficiently with the genotype of Mus musculus domesticus x M. m. molossinus. Thus, injection of two round spermatid nuclei followed by maternal enucleation is an effective alternative method of producing androgenetic embryos that consistently develop into blastocysts and mid-gestation fetuses.
authors.affiliation: RIKEN Bioresource Center, Tsukuba, Ibaraki, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miki
authors.forename: Michiko
authors.initials: M
authors.lastname: Hirose
authors.forename: Narumi
authors.initials: N
authors.lastname: Ogonuki
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.forename: Fuyuko
authors.initials: F
authors.lastname: Kezuka
authors.forename: Arata
authors.initials: A
authors.lastname: Honda
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Mekada
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Hanaki
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Iwafune
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yoshiki
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Ishino
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D004622:Embryo, Mammalian; D005260:Female; D008297:Male; D051379:Mice; D053652:Nuclear Transfer Techniques; D009865:Oocytes; D013087:Spermatids