In vitro antioxidant, antimicrobial and anti-herpes simplex virus type 1 activity of Phellodendron amurense Rupr. from China.
Phellodendron amurense Rupr. bark extracts were examined for antioxidant activity, antimicrobial activity and antiviral activity on herpes simplex virus type 1 (HSV-1). Ethanol extract showed higher content of both total phenolic and flavonoid than aqueous extract. In DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay, the concentration providing 50% inhibition (IC(50)) values were 6.73 +/- 0.87 mg/ml and 4.26 +/- 0.59 mg/ml for aqueous and ethanol extracts respectively. Ethanol extract had a much higher antimicrobial activity than aqueous extract. Furthermore, the antiviral activity of the ethanol extract on HSV-1 was also tested. The maximum noncytotoxic concentration was 44.12 microg/ml for RC-37 cells. Plaque formation was inhibited by 74+/-6% when HSV-1 was pretreated with the extract prior to adsorption, whereas pretreatment of the cells with the extract, added during adsorption or after the adsorption only exhibited above 10% antiviral effect. This study has to some extent validated the medicinal potential of P. amurense bark.
Wang
Wei
W
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Hexing Road 26, 150040 Harbin, China.
Zu
Yuangang
Y
Fu
Yujie
Y
Reichling
Jürgen
J
Suschke
Ulrike
U
Nokemper
Silke
S
Zhang
Yuhong
Y
D000212:Acyclovir; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002460:Cell Line; D002681:China; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D032102:Phellodendron; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D000212:Acyclovir