title: A 28-day repeat dose toxicity study of steroidal glycoalkaloids, alpha-solanine and alpha-chaconine in the Syrian Golden hamster.
abstract: Glycoalkaloids alpha-solanine and alpha-chaconine are naturally present toxicants in the potato plant (Solanumtuberosum). Human intake of high doses of glycoalkaloids has led to acute intoxication, in severe cases coma and death. Previous studies have indicated that the ratio of alpha-solanine to alpha-chaconine may determine the degree and nature of the glycoalkaloid toxicity in potatoes, as the toxicity of the two alkaloids act synergistically. The aim of the present study was to investigate whether an altered ratio of alpha-solanine and alpha-chaconine would reduce the toxicity of the glycoalkaloids. The Syrian Golden hamster was given daily doses of alpha-solanine and alpha-chaconine by gavage for 28 days. Doses of up to 33.3 mg total glycoalkaloids/kg body weight were applied in ratios of 1:3.7 and 1:70 (alpha-solanine:alpha-chaconine). Administration of the highest doses of both ratios resulted in distended and fluid filled small intestines and stomach. Animals receiving the ratio with the reduced content of alpha-solanine were less affected compared to those receiving the other ratio. Gene expression profiling experiments were conducted using RNA from epithelial scrapings from the small intestines of the hamsters administered the highest doses of the glycoalkaloid treatments. In general, more differential gene expression was observed in the epithelial scrapings of the hamsters fed the ratio of 1:3.7. Mostly, pathways involved in lipid and energy metabolism were affected by the ratio of 1:3.7.
authors.affiliation: The National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Langkilde
authors.forename: Tafadzwa
authors.initials: T
authors.lastname: Mandimika
authors.forename: Malene
authors.initials: M
authors.lastname: Schrøder
authors.forename: Otto
authors.initials: O
authors.lastname: Meyer
authors.forename: Wout
authors.initials: W
authors.lastname: Slob
authors.forename: Ad
authors.initials: A
authors.lastname: Peijnenburg
authors.forename: Morten
authors.initials: M
authors.lastname: Poulsen
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001682:Biological Availability; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D002091:Butyrylcholinesterase / Q000097:blood / Q000378:metabolism; D002802:Cholinesterases / Q000097:blood; D006224:Cricetinae; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D007441:Intubation, Gastrointestinal; D008647:Mesocricetus; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012992:Solanine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000633:toxicity; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: C514919:alpha-solanine; D012992:Solanine; C011860:alpha-chaconine; D012313:RNA; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases