Development of the light sensitivity of the clock genes Period1 and Period2, and immediate-early gene c-fos within the rat suprachiasmatic nucleus.
The molecular mechanism underlying circadian rhythmicity within the suprachiasmatic nuclei (SCN) of the hypothalamus has two light-sensitive components, namely the clock genes Per1 and Per2. Besides, light induces the immediate-early gene c-fos. In adult rats, expression of all three genes is induced by light administered during the subjective night but not subjective day. The aim of the present study was to ascertain when and where within the SCN the photic sensitivity of Per1, Per2 and c-fos develops during early postnatal ontogenesis. The specific aim was to find out when the circadian clock starts to gate photic sensitivity. The effect of a light pulse administered during either the subjective day or the first or second part of the subjective night on gene expression within the rat SCN was determined at postnatal days (P) 1, 3, 5 and 10. Per1, Per2 and c-fos mRNA levels were assessed 30 min, 1 and 2 h after the start of each light pulse by in situ hybridization histochemistry. Expression of Per1 and c-fos was light responsive from P1, and the responses began to be gated by the circadian clock at P3 and P10, respectively. Expression of Per2 was only slightly light responsive at P3, and the response was not fully gated until P5. These data demonstrate that the light sensitivity of the circadian clock develops gradually during postnatal ontogenesis before the circadian clock starts to control the response. The photoinduction of the clock gene Per2 develops later than that of Per1.
Matejů
Kristýna
K
Institute of Physiology, vvi, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Bendová
Zdena
Z
El-Hennamy
Rehab
R
Sládek
Martin
M
Sosniyenko
Serhiy
S
Sumová
Alena
A
D000375:Aging / Q000235:genetics / Q000528:radiation effects; D000818:Animals; D000831:Animals, Newborn; D001683:Biological Clocks / Q000235:genetics* / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology / Q000528:radiation effects; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D055537:Light Signal Transduction / Q000235:genetics / Q000528:radiation effects; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000528:radiation effects; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D056950:Period Circadian Proteins; D010775:Photic Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D012333:RNA, Messenger / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000378:metabolism* / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; C539024:Per1 protein, rat; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014157:Transcription Factors