Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase.
BACKGROUND
Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme.


METHODS
Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 microg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px.


RESULTS
Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein.


CONCLUSIONS
The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.
Zachara
Bronislaw A
BA
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland. bronzach@yahoo.com
Gromadzinska
Jolanta
J
Zbrog
Zbigniew
Z
Swiech
Rafal
R
Wasowicz
Wojciech
W
Twardowska
Ewa
E
Jablonska
Ewa
E
Sobala
Wojciech
W
D004311:Double-Blind Method; D004790:Enzyme Induction; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology / Q000628:therapy*; D010919:Placebos; D006435:Renal Dialysis; D012643:Selenium / Q000008:administration & dosage*; D013054:Spectrophotometry, Atomic
D010919:Placebos; D005979:Glutathione Peroxidase; D012643:Selenium