Umbilical cord blood cell-derived neurospheres differentiate into Schwann-like cells.
In this study, we examined the phenotypic and bioassay characteristics of human umbilical cord blood-derived mesenchymal stromal cells (UCB-MSCs) differentiated along a Schwann cells lineage. Initially, we induced human UCB-MSCs into floating neurospheres, and then, neurospheres were induced to differentiate into Schwann-like cells using glia growth factors. Differentiated UCB-MSCs showed morphological changes similar to those of Schwann cells. Expression of the Schwann cell markers was determined by immunocytochemical staining and western blotting. Furthermore, differentiated UCB-MSCs could promote neurite outgrowth in coculture with dorsal root ganglia neurons. These results show that UCB-MSCs can be differentiated into cells that are Schwann-like in terms of morphology, phenotype, and function.
Zhang
Hong-Tian
HT
Department of Neurosurgery, Zhujiang Hospital, China.
Cheng
Hao-Yu
HY
Zhang
Liang
L
Fan
Juan
J
Chen
Yi-Zhao
YZ
Jiang
Xiao-Dan
XD
Xu
Ru-Xiang
RX
D000704:Analysis of Variance; D015415:Biomarkers; D015153:Blotting, Western; D002478:Cells, Cultured; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology*; D005727:Ganglia, Spinal / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D016501:Neurites / Q000502:physiology; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D009418:S100 Proteins; C498585:TUBB3 protein, human; D014404:Tubulin