Evaluation of the agreement among three handheld blood glucose meters and a laboratory blood analyzer for measurement of blood glucose concentration in Hispaniolan Amazon parrots (Amazona ventralis).
OBJECTIVE
To determine the degree of agreement between 3 commercially available point-of-care blood glucose meters and a laboratory analyzer for measurement of blood glucose concentrations in Hispaniolan Amazon parrots (Amazona ventralis).


METHODS
20 healthy adult Hispaniolan Amazon parrots.


METHODS
A 26-gauge needle and 3-mL syringe were used to obtain a blood sample (approx 0.5 mL) from a jugular vein of each parrot. Small volumes of blood (0.6 to 1.5 microL) were used to operate each of the blood glucose meters, and the remainder was placed into lithium heparin microtubes and centrifuged. Plasma was harvested and frozen at -30 degrees C. Within 5 days after collection, plasma samples were thawed and plasma glucose concentrations were measured by means of the laboratory analyzer. Agreement between pairs of blood glucose meters and between each blood glucose meter and the laboratory analyzer was evaluated by means of the Bland-Altman method, and limits of agreement (LOA) were calculated.


RESULTS
None of the results of the 3 blood glucose meters agreed with results of the laboratory analyzer. Each point-of-care blood glucose meter underestimated the blood glucose concentration, and the degree of negative bias was not consistent (meter A bias, -94.9 mg/dL [LOA, -148.0 to -41.7 mg/dL]; meter B bias, -52 mg/dL [LOA, -107.5 to 3.5 mg/dL]; and meter C bias, -78.9 mg/dL [LOA, -137.2 to -20.6 mg/dL]).


CONCLUSIONS
On the basis of these results, use of handheld blood glucose meters in the diagnosis or treatment of Hispaniolan Amazon parrots and other psittacines cannot be recommended.
Acierno
Mark J
MJ
Department of Veterinary Clinical Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70810, USA.
Mitchell
Mark A
MA
Schuster
Patricia J
PJ
Freeman
Diana
D
Sanchez-Migallon Guzman
David
D
Tully
Thomas N
TN
D046708:Amazona / Q000097:blood*; D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000662:veterinary*; D001786:Blood Glucose / Q000032:analysis*; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000662:veterinary*; D015203:Reproducibility of Results
D001786:Blood Glucose