title: Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins.
abstract: Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.
authors.affiliation: Department of Microbiology and Immunology, WHO Collaborating Centre for Research on Enterotoxigenic Escherichia coli, Institute of Biomedicine, University of Gothenburg, Göteborg, Sweden. claudia.rodas@microbio.gu.se
authors.forename: Claudia
authors.initials: C
authors.lastname: Rodas
authors.forename: Volga
authors.initials: V
authors.lastname: Iniguez
authors.forename: Firdausi
authors.initials: F
authors.lastname: Qadri
authors.forename: Gudrun
authors.initials: G
authors.lastname: Wiklund
authors.forename: Ann-Mari
authors.initials: AM
authors.lastname: Svennerholm
authors.forename: Asa
authors.initials: A
authors.lastname: Sjöling
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D001459:Bangladesh; D001838:Bolivia; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D037521:Virulence Factors