title: Analysis of impurities in streptomycin and dihydrostreptomycin by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time-of-flight mass spectrometry.
abstract: Impurities in streptomycin (STR) and dihydrostreptomycin (DHS) were investigated by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time-of-flight mass spectrometry (HILIC/ESI-QIT/TOFMS). Samples were separated on a fused-core silica column (100 mmx2.1 mm i.d., particle size: 2.7 microm) with isocratic elution using 200 mM ammonium formate buffer (pH 4.5) and acetonitrile as mobile phase. Constant neutral loss survey in accurate mass measurement was carried out by QIT/TOFMS. Formulae, chemical structures of impurities in an STR sample were suggested with supporting results on the probable pathways of STR biosynthesis by Streptomyces griseus.
authors.affiliation: Analytical Applications Department, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. kawashin@shimadzu.co.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kawano
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis / Q000737:chemistry; D004340:Drug Contamination / Q000517:prevention & control*; D057927:Hydrophobic and Hydrophilic Interactions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013307:Streptomycin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin