title: Identification of the csp gene and molecular modelling of the CspA-like protein from Antarctic soil-dwelling psychrotrophic bacterium Psychrobacter sp. B6.
abstract: We cloned and sequenced the cspA-like gene from a psychrotrophic Antarctic soil-dwelling bacterial strain Psychrobacter sp. B6. The gene is 213 bp long and shows 99% and 98% sequence identity with the Psychrobacter cryohalolentis K5 gene encoding a cold-shock DNA-binding domain protein and the Psychrobacter arcticus transcriptional regulator-CspA gene, respectively. The protein encoded by the Psychrobacter sp. B6 cspA-like gene shows 100% identity with the two proteins mentioned above, and also 61% sequence identity with CspB from Bacillus subtilis and Csp from Bacillus caldolyticus, and 56% - with Escherichia coli CspA protein. A three-dimensional model of the CspA-like protein from Psychrobacter sp. B6 was generated based on three known structures of cold shock proteins: the crystal structure of the major cold shock protein from Escherichia coli (CspA), the NMR structure of the latter protein, and the NMR structure of Csp from Thermotoga maritima. The deduced structure of the CspA-like protein from Psychrobacter sp. B6 was found to be very similar to these known structures of Csp-like proteins.
authors.affiliation: Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland. agakauf@yahoo.com
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kaufman-Szymczyk
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Wojtasik
authors.forename: Paweł
authors.initials: P
authors.lastname: Parniewski
authors.forename: Aneta
authors.initials: A
authors.lastname: Białkowska
authors.forename: Karolina
authors.initials: K
authors.lastname: Tkaczuk
authors.forename: Marianna
authors.initials: M
authors.lastname: Turkiewicz
mesh_terms: D000595:Amino Acid Sequence; D000864:Antarctic Regions; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D044096:Psychrobacter / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial