IL-1beta in irrigation fluid and mRNA expression in synovial tissue of the knee joint as therapeutic markers of inflammation in collagen antibody-induced arthritis.
OBJECTIVE
We studied the relationship between the severity of inflammation and IL-1beta production and relative expression level of IL-1beta mRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of a murine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markers for treating rheumatoid arthritis.


METHODS
Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIA induction. The IL-1beta protein level was measured by enzyme-linked immunosorbent assay, and the relative expression level of IL-1beta mRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levels and histopathological changes.


RESULTS
IL-1beta concentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. The relative expression level of IL-1beta mRNA was elevated in synovial tissue. Histopathological changes in the knee joint and foot indicated similar severity.


CONCLUSIONS
IL-1beta concentration in irrigation fluid and relative expression level of IL-1beta mRNA in the synovium have potential as therapeutic markers in studying and treating CAIA.
Chia
Wei-Tso
WT
Department of Health, Taiwan Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
Yeh
Li-Tzu
LT
Chen
Yuan-Wu
YW
Lee
Herng-Sheng
HS
Chang
Deh-Ming
DM
Sytwu
Huey-Kang
HK
D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D007596:Joints / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013582:Synovial Fluid / Q000276:immunology; D013583:Synovial Membrane / Q000276:immunology
D015415:Biomarkers; D017931:DNA Primers; D053583:Interleukin-1beta; D012333:RNA, Messenger