title: Construction and characterization of a high activity mutant of human keratinocyte growth factor-2.
abstract: Keratinocyte growth factor-2 (KGF-2) plays an important role in vertebrate limb development, lung branching morphogenesis, regeneration and reconstruction of the epidermis. Previous studies have used the wild type factor. Here, we have constructed a double-site mutant of human KGF-2, named STEA. STEA possesses higher receptor binding affinity and promotes better proliferation activity on rat tracheal epithelium (RTE) cells than recombinant human KGF-2. These results suggest that the simultaneous mutation of Ser115 to Thr and Glu117 to Ala improves the biological activity of KGF-2.
authors.affiliation: Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, 100850 Beijing, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Cai
authors.forename: Minji
authors.initials: M
authors.lastname: Zou
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Shen
authors.initials: S
authors.lastname: Liu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
authors.forename: Donggang
authors.initials: D
authors.lastname: Xu
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000494:pharmacology*; D020125:Mutation, Missense; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects
chemical_list: D051526:Fibroblast Growth Factor 10; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins