Photothermal absorption correlation spectroscopy.
Fluorescence correlation spectroscopy (FCS) is a popular technique, complementary to cell imaging for the investigation of dynamic processes in living cells. Based on fluorescence, this single molecule method suffers from artifacts originating from the poor fluorophore photophysics: photobleaching, blinking, and saturation. To circumvent these limitations we present here a new correlation method called photothermal absorption correlation spectroscopy (PhACS) which relies on the absorption properties of tiny nano-objects. PhACS is based on the photothermal heterodyne detection technique and measures akin FCS, the time correlation function of the detected signals. Application of this technique to the precise determination of the hydrodynamic sizes of different functionalized gold nanoparticles are presented, highlighting the potential of this method.
Octeau
Vivien
V
Centre de Physique Moleculaire Optique et Hertzienne, Universite de Bordeaux and CNRS, 351 cours de la Liberation, Talence, F-33405, France.
Cognet
Laurent
L
Duchesne
Laurence
L
Lasne
David
D
Schaeffer
Nicolas
N
Fernig
David G
DG
Lounis
Brahim
B
D000042:Absorption; D004058:Diffusion; D006046:Gold / Q000737:chemistry; D008027:Light; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry; D009038:Motion; D055668:Photochemical Processes; D013057:Spectrum Analysis / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D008565:Membrane Proteins; D006046:Gold