[Changes of ICAM-1 mRNA expression in rats' skin and muscle after contusion].
OBJECTIVE
To investigate the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA in contused skin and muscle of rats and the relationship between the ICAM-1 expression and the wound age.


METHODS
The samples were taken at 0.5, 1, 6, 12, 18, 24 and 30 h after contusion of rats, respectively. Total RNA was extracted both from the skin and muscle samples of each group and reverse transcription polymerase chain reaction (RT-PCR) was conducted to synthesize the 1st strand cDNA. The amount of ICAM-1 mRNA in each sample was quantified using rp132 intrinsic fluorescent assay and compared by the 2 (-Delta Delta Ct) method with that from control samples.


RESULTS
After contusion the expression of ICAM-1 mRNA in skin increased rapidly and peaked at 0.5 h, at 24 h degraded to the amount that was seven times as much as the control group, then rised again. The expression of ICAM-1 mRNA in muscle increased significantly within 0.5 h and peaked at 6 h, reached the minimum at 18 h, then increased again.


CONCLUSIONS
It is suggested that ICAM-1 mRNA analysis may be useful for estimation of early wound age because of its time-related expression after contusion in skin and muscle.
Sun
Jun-Hong
JH
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. sunjunhong146@163.com
Du
Qiu-Xiang
QX
Wang
Xiao-Wei
XW
Wang
Ying-Yuan
YY
D000818:Animals; D003288:Contusions / Q000378:metabolism*; D005260:Female; D005554:Forensic Medicine; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D013997:Time Factors
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1