title: ITAM signaling by Vav family Rho guanine nucleotide exchange factors regulates interstitial transit rates of neutrophils in vivo.
abstract: BACKGROUND
  In response to infection, neutrophils are quickly recruited from the blood into inflamed tissues. The interstitial migration of neutrophils is crucial for the efficient capture and control of rapidly proliferating microbes before microbial growth can overwhelm the host's defenses. However, the molecular mechanisms that regulate interstitial migration are incompletely understood.
  
  
  RESULTS
  Here, we use two-photon microscopy (2PM) to study discrete steps of neutrophil responses during subcutaneous infection with bacteria. Our study demonstrates that signals emanating from ITAM-containing receptors mediated by Vav family Rho GEFs control the velocity, but not the directionality, of neutrophil migration towards sites of bacterial infection.
  
  
  CONCLUSIONS
  Here we show that during neutrophil migration towards sites of bacterial infection, signals emanating from ITAM-containing receptors specifically control interstitial neutrophil velocity.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, United States of America.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Graham
authors.forename: Bernd H
authors.initials: BH
authors.lastname: Zinselmeyer
authors.forename: Francesca
authors.initials: F
authors.lastname: Mascarenhas
authors.forename: Ryan
authors.initials: R
authors.lastname: Delgado
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Miller
authors.forename: Wojciech
authors.initials: W
authors.lastname: Swat
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D051379:Mice; D009504:Neutrophils / Q000166:cytology*; D051567:Proto-Oncogene Proteins c-vav / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D051567:Proto-Oncogene Proteins c-vav