title: Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes.
abstract: Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Igarashi
authors.forename: Kousuke
authors.initials: K
authors.lastname: Yamagata
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Sugai
authors.forename: Yukari
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Emiko
authors.initials: E
authors.lastname: Sugawara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tamura
authors.forename: Hajime
authors.initials: H
authors.lastname: Yaegashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Takahashi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Isogai
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshikawa
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D019662:Cucurbitaceae / Q000235:genetics; D007887:Fabaceae / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D020868:Gene Silencing; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D008190:Lyases / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D012328:RNA Viruses / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*
chemical_list: D018749:RNA, Plant; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; D008190:Lyases; C086788:magnesium chelatase