Single nanoparticle tracking-based detection of membrane receptor-ligand interactions.
We developed a single nanoparticle tracking-based detection method for membrane-associated molecules using a paucivalent gold nanoparticle (AuNP)-modified supported lipid bilayer (SLB) platform. Here, the binding activity of membrane-associated molecules (cholera toxin binding to ganglioside GM(1) in this case) was determined by calculating the diffusion coefficients of membrane-tethered AuNPs. This nonbleaching nanoparticle-based method provides >100-fold improvement in sensitivity for the same target without optimization over the fluorophore-based method that also has photobleaching and photoblinking problems. This new detection platform and analysis method could be used for membrane-associated molecule biosensor and screening assay development.
Yang
Yun-Hee
YH
Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-747, South Korea.
Nam
Jwa-Min
JM
D015374:Biosensing Techniques; D002462:Cell Membrane / Q000378:metabolism*; D002772:Cholera Toxin / Q000032:analysis / Q000378:metabolism; D006046:Gold / Q000032:analysis / Q000737:chemistry; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry; D009038:Motion; D011485:Protein Binding; D014734:Vibrio cholerae
D008024:Ligands; D008051:Lipid Bilayers; D006046:Gold; D002772:Cholera Toxin