Biological control of corky root in tomato.
Corky root caused by Pyrenochaeta lycopersici (Schneider et Gerlach) is one of the most important soil borne fungal pathogens which develops in the soils, causing diseases in different crops. The research was carried out to evaluate the effectiveness of the biological control of corky root on tomato. Biological control was performed by using Trichoderma viride Pers. 18/17 SS, Streptomyces spp. AtB42 and Bacillus subtilis M51 PI. According to present and future regulations on the use of chemical fungicides and considering that treatments must avoids environmental pollution, the main object of this research was to find alternative strategies by using biocontrol agents against P. lycopersici that affect tomato plants. In laboratory, the effectiveness of T. viride 18/17 SS, Streptomyces spp. AtB42 and B. subtilis M51 PI to control P. lycopersici were studied. In greenhouse, the research was carried out comparing the following treatments: 1) untreated control; 2) T. viride 18/17 SS; 3) Streptomyces spp. AtB42; 4) B. subtilis M51 PI. Roots of plants of tomato H3028 Hazera were treated with the antagonist suspensions just prior of transplant. Treatments were repeated about 2 months after, with the same suspensions sprayed on the soil to the plant collar. In dual culture, the inhibition of P. lycopersici ranged up to 81.2% (caused from T. viride 18/17 SS), 75.6% (from Streptomyces spp. AtB42) and 66.8% (from B. subtilis M51 PI). In greenhouse trials, with regard to corky root symptoms, all treated plots showed signifycative differences compared to untreated. T. viride gave the better results followed by Streptomyces spp. and then by B. subtilis. The fungus antagonist showed good root surface competence such as demonstrated its persistence on the roots surface of the tomato plants whose roots were treated with T. viride 18/17 SS up to 2 months before.
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Centro di Ricerca per l'Orticoltura (CRA - Roma), via Cavalleggeri 25, IT-84098 Pontecagnano (Salerno), Italy.
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D000898:Antibiosis; D001412:Bacillus subtilis / Q000502:physiology*; D018920:Coculture Techniques; D015169:Colony Count, Microbial; D018551:Solanum lycopersicum / Q000382:microbiology*; D003904:Mitosporic Fungi / Q000254:growth & development* / Q000472:pathogenicity; D010572:Pest Control, Biological / Q000379:methods*; D018517:Plant Roots / Q000382:microbiology; D011897:Random Allocation; D012988:Soil Microbiology; D013302:Streptomyces / Q000502:physiology*; D014242:Trichoderma / Q000502:physiology*