Multivalent protein binding and precipitation by self-assembling molecules on a DNA pentaplex scaffold.
A supramolecular assembly containing an isoguanosine pentaplex with both a "protein-binding" face and a "reporter" face has been generated. When phosphocholine is appended to the protein-binding face this supramolecular assembly binds multivalently to the pentameric human C-reactive protein, a biomolecule implicated in inflammation and heart disease.
Rosenzweig
Brooke A
BA
Department of Chemistry, Yale University, P.O. Box 208107, New Haven, Connecticut 06511, USA.
Ross
Nathan T
NT
Tagore
Debarati M
DM
Jayawickramarajah
Janarthanan
J
Saraogi
Ishu
I
Hamilton
Andrew D
AD
D002097:C-Reactive Protein / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins; D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008027:Light; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism*; D012542:Scattering, Radiation
D002352:Carrier Proteins; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D004247:DNA