Immunohistochemical expression of matrix metalloproteinases 1, 2, and 9 in odontogenic myxoma and dental germ papilla.
The aim of this study was to compare the immunohistochemical expression of matrix metalloproteinases (MMPs) 1, 2, and 9 in odontogenic myxomas and dental germ papillae. Twelve cases of odontogenic myxoma and eight tooth germ specimens were selected for analysis of the immunohistochemical expression and the pattern of distribution of MMPs 1, 2, and 9 in extracellular matrix (ECM), as well as of the number of MMP-positive cells. MMP-2 was expressed only in the ECM of myxomas (p<0.05). No significant difference was observed between ECM immunoreactivity for MMP-9 in myxomas and dental papillae (p>0.05). MMP-1 immunoreactivity was detected in most myxoma cases at a proportion similar to that observed in dental papillae (p>0.05). A significant difference was observed in the number of immunoreactive cells in myxomas (p<0.05), MMP-1 being present at higher proportions than MMPs 2 and 9. There was a gradient in the expression of MMPs in the ECM and in neoplastic cells of odontogenic myxomas, with higher immunoreactivity to MMP-1 and lower immunoreactivity to MMP-9. Taken together, our results suggest the existence of a coordinated mechanism between MMPs 1, 2, and 9 that aimed at the efficient degradation of extracellular matrix in odontogenic myxomas.
Nonaka
Cassiano Francisco Weege
CF
Department of Oral Pathology, Dentistry School, Av. Senador Salgado Filho, 1787, Federal University of Rio Grande do Norte, Lagoa Nova, Natal, Rio Grande do Norte CEP 59056-000, Brazil.
Augusto Vianna Goulart Filho
João
J
Cristina da Costa Miguel
Márcia
M
Batista de Souza
Lélia
L
Pereira Pinto
Leão
L
D003771:Dental Papilla / Q000201:enzymology*; D005109:Extracellular Matrix / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000201:enzymology* / Q000473:pathology; D020781:Matrix Metalloproteinase 1 / Q000032:analysis*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D009232:Myxoma / Q000201:enzymology* / Q000473:pathology; D009808:Odontogenic Tumors / Q000201:enzymology* / Q000473:pathology
C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1