Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China.
In the present study, a genotype 4 HEV strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in Shanghai Children's hospital. The full capsid protein gene (ORF2) sequence of this strain was determined by RT-PCR method. Sequence analysis based on the full ORF2 sequence indicated that this HEV strain shared the highest sequence identity (97.6%) with another human HEV strain isolated from a Japanese patient who was infected by genotype 4 HEV during traveling in Shanghai. Phylogenetic analysis showed that this genotype 4 HEV was phylogenetically far from the genotype 4 HEV strain that was commonly prevalent in Shanghai swine group, suggesting that this strain may not come from swine group and not involved in zoonotic transmission in this area.
Zhang
Wen
W
Zoonosis and Comparative Medicine Group, School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai, PR China. z0216wen@sjtu.edu.cn
Yang
Shixing
S
Shen
Quan
Q
Liu
Junfeng
J
Shan
Tongling
T
Huang
Fen
F
Ning
Huibo
H
Kang
Yanjun
Y
Yang
Zhibiao
Z
Cui
Li
L
Zhu
Jianguo
J
Hua
Xiuguo
X
D002681:China; D005243:Feces / Q000821:virology; D005838:Genotype; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
C400971:ORF2 protein, Hepatitis E virus; D014764:Viral Proteins