Role of NMDA receptor upon [14C]acetate uptake into intact rat brain.
OBJECTIVE
To clarify the role of N-methyl-D: -aspartate (NMDA) receptors upon [(14)C]acetate uptake in the rodent central nervous system (CNS), ibotenic acid (IBO) was infused into the right striatum of the rat brain.


METHODS
Autoradiograms of [(14)C]acetate uptake in the brain for 2 h following the infusion of IBO (10 microg/microl) were obtained in both non-treated and MK-801 (1 mg/kg, i.v.) pretreated rats. The effect of MK-801 on [(14)C]acetate uptake in the normal rat brain was also studied.


RESULTS
Infusion of IBO significantly decreased [(14)C]acetate uptake in the infused side of the striatum. The expression of monocarboxylate transporter-1 was not altered, suggesting that the activity of tricarboxylic acid (TCA) cycle in glial cells might be depressed. Pretreatment with MK-801 completely blocked the decreasing effect of IBO on [(14)C]acetate uptake. MK-801 also increased [(14)C]acetate uptake in the whole brain of normal rats.


CONCLUSIONS
These results indicate the important roles of NMDA receptors on [(14)C]acetate uptake in the intact rat brain.
Hirose
Shinichiro
S
Division of Health Sciences, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. hirose@sahs.med.osaka-u.ac.jp
Momosaki
Sotaro
S
Hosoi
Rie
R
Abe
Kohji
K
Gee
Antony
A
Inoue
Osamu
O
D000085:Acetates / Q000493:pharmacokinetics*; D000818:Animals; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D003342:Corpus Striatum / Q000000981:diagnostic imaging* / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000008:administration & dosage; D007051:Ibotenic Acid / Q000008:administration & dosage; D008297:Male; D008657:Metabolic Clearance Rate; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014018:Tissue Distribution
D000085:Acetates; D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals; D016194:Receptors, N-Methyl-D-Aspartate; D007051:Ibotenic Acid; D016291:Dizocilpine Maleate