title: Inhibition of inflammatory mediators by polyphenolic plant extracts in human intestinal Caco-2 cells.
abstract: The mitogen-activated protein kinases (MAPK) and nuclear factor kappaB (NF-kappaB) are involved in transduction cascades that play a key role in inflammatory response. We tested the ability of preselected natural polyphenolic extracts (grape seed, cocoa, sugar cane, oak, mangosteen and pomegranate) to modulate intestinal inflammation using human intestinal Caco-2 cells treated for 4h with these extracts and then stimulated by cytokines for 24 or 48h. The effect of polyphenolic extracts, at 50 micromol of gallic acid equivalent/l, was investigated on inflammation-related cellular events: (i) NF-kappaB activity (cells transfected with a NF-kappaB-luciferase construct), (ii) activation of Erk1/2 and JNK (western blotting), (iii) secretion of interleukin 8 (IL-8) (ELISA), (iv) secretion of prostaglandin (PG) E(2) (ELISA), (v) production of NO (Griess method). Results show that: (i) sugar cane, oak and pomegranate extracts inhibited NF-kappaB activity (from 1.6 to 1.9-fold) (P<0.001); (ii) pomegranate slightly inhibited Erk1/2 activation (1.3-fold) (P=0.008); (iii) oak and pomegranate decreased NO synthesis by 1.5-fold (P<0.001) and that of IL-8 by 10.3 and 6.7-fold respectively; (iv) pomegranate and cocoa decreased PGE(2) synthesis by 4.6 (P<0.0001) and 2.2-fold (P=0.001), respectively. We suggest that pomegranate extract could be particularly promising in dietary prevention of intestinal inflammation.
authors.affiliation: Biochimie Cellulaire, Nutritionnelle et Toxicologique, Institut des Sciences de la Vie, Université Catholique de Louvain, Croix du Sud, 5, B 1348 Louvain-la-Neuve, Belgium.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Romier-Crouzet
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Van De Walle
authors.forename: Alexandrine
authors.initials: A
authors.lastname: During
authors.forename: Aurélie
authors.initials: A
authors.lastname: Joly
authors.forename: Charline
authors.initials: C
authors.lastname: Rousseau
authors.forename: Olivier
authors.initials: O
authors.lastname: Henry
authors.forename: Yvan
authors.initials: Y
authors.lastname: Larondelle
authors.forename: Yves-Jacques
authors.initials: YJ
authors.lastname: Schneider
mesh_terms: D000578:Amidines; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry; D015232:Dinoprostone / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D016209:Interleukin-8 / Q000096:biosynthesis; D008077:Lipoproteins, LDL / Q000737:chemistry; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009569:Nitric Oxide / Q000096:biosynthesis; D010084:Oxidation-Reduction; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D010944:Plants / Q000737:chemistry*; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D000578:Amidines; D000975:Antioxidants; D005419:Flavonoids; D005609:Free Radicals; D018836:Inflammation Mediators; D016209:Interleukin-8; D008077:Lipoproteins, LDL; D016328:NF-kappa B; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; C046728:2,2'-azobis(2-amidinopropane); D003300:Copper; D020928:Mitogen-Activated Protein Kinases; D015232:Dinoprostone