Role of CD4(+) T cells in the modulation of neurotrophin production in mice exposed to low-level toluene.
To investigate the role of CD4(+) T cells in neurotrophin production following toluene exposure, male C3H mice were exposed to filtered air (control) or 9 ppm of toluene in a nose-only exposure chamber for 30 min on 3 consecutive days followed by weekly sessions for 4 weeks. All the mice were immunized with ovalbumin and some groups of mice were treated with anti-CD4 antibody. BDNF content in BAL fluid and NGF content in plasma were significantly increased in toluene-exposed mice. However, treatment with anti-CD4 mAb completely abrogated these effects. These findings suggest that the CD4(+) T cells may be involved in the toluene-induced modulation of neurotrophin production.
Fujimaki
Hidekazu
H
National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan. fujimaki@nies.go.jp
Win-Shwe
Tin-Tin
TT
Yamamoto
Shoji
S
Nakajima
Daisuke
D
Goto
Sumio
S
D000280:Administration, Inhalation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D002452:Cell Count; D007114:Immunization; D016676:Macrophages, Alveolar / Q000166:cytology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D020932:Nerve Growth Factor / Q000097:blood / Q000378:metabolism; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000378:metabolism; D015213:Neuroimmunomodulation / Q000187:drug effects* / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014050:Toluene / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D019208:Brain-Derived Neurotrophic Factor; D015704:CD4 Antigens; D009414:Nerve Growth Factors; D014050:Toluene; D010047:Ovalbumin; D020932:Nerve Growth Factor