title: Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane.
abstract: We report our experience with Gnathostoma protein preparation by the ultrafiltration method. Crude antigen was sonicated and ultrafiltrated using the Nanosep 100 K membrane. SDS-PAGE electrophoresis showed protein bands at 43, 41, 24, 22, 21, 19.5 kDa. Use of the ultrafiltration method can provide specific protein (24 kDa), similar to the non-ultrafiltration method, with the other 5 non-specific proteins. Using the non-ultrafiltration method, there were more (20) non-specific protein. The ultrafiltration method can be an alternative method for the preparation of protein, which can provide better results than non-ultrafiltration.
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Suphan
authors.initials: S
authors.lastname: Sugaroon
authors.forename: Wilai
authors.initials: W
authors.lastname: Saksirisampant
authors.forename: Kanyarattana
authors.initials: K
authors.lastname: Kraivichian
authors.forename: Jamsai
authors.initials: J
authors.lastname: Suwansaksri
authors.forename: Viroj
authors.initials: V
authors.lastname: Wiwanitkit
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D045424:Complex Mixtures / Q000737:chemistry; D006039:Gnathostoma / Q000276:immunology*; D015801:Helminth Proteins / Q000302:isolation & purification*; D008567:Membranes, Artificial; D036103:Nanotechnology; D014462:Ultrafiltration
chemical_list: D000947:Antigens, Helminth; D045424:Complex Mixtures; D015801:Helminth Proteins; D008567:Membranes, Artificial