title: PTGDR is not a major candidate gene for asthma and atopy in Chinese children.
abstract: Airway sensitization requires the expression of prostanoid DP receptor in mice. Recent studies reported that polymorphisms in the gene encoding prostanoid DP receptor (PTGDR) were associated with asthma in White people and Black people, but this association could not be replicated among Latinos and Koreans. This study investigated the association between asthma-related traits and six single nucleotide polymorphisms (SNPs) of PTGDR in Chinese children, consisted of 308 asthmatics and 368 non-allergic controls. Plasma total and aeroallergen-specific immunoglobulin E were measured by immunoassays. PTGDR SNPs were determined by multiplex SNaPshot genotyping. All polymorphic markers followed Hardy-Weinberg equilibrium except G1044A in the controls (p = 0.021). The linkage disequilibrium (LD) scores for these SNPs were moderate to high, and in particular, T-549C and C-441T were in strong LD. Significant interethnic variations in PTGDR alleles and haplotypes (up to 41%) were found in our subjects when compared with White people or Latinos. Asthma diagnosis, atopy and aeroallergen sensitization did not differ among children with different PTGDR genotypes (p > 0.15 for all). Linear regression showed weakly significant associations between T-197C and G1044A of PTGDR and spirometric variables. PTGDR haplotypes were not associated with asthma and atopy phenotypes (p > 0.09 for all). Our results do not support PTGDR to be a major candidate gene for asthma traits in Chinese children.
authors.affiliation: Department of Paediatrics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. tfleung@cuhk.edu.hk
authors.forename: Ting Fan
authors.initials: TF
authors.lastname: Leung
authors.forename: Chung Yi
authors.initials: CY
authors.lastname: Li
authors.forename: Alice P S
authors.initials: AP
authors.lastname: Kong
authors.forename: Iris H S
authors.initials: IH
authors.lastname: Chan
authors.forename: Maggie C Y
authors.initials: MC
authors.lastname: Ng
authors.forename: Michael H M
authors.initials: MH
authors.lastname: Chan
authors.forename: Wing Yee
authors.initials: WY
authors.lastname: So
authors.forename: Gary W K
authors.initials: GW
authors.lastname: Wong
authors.forename: Christopher W K
authors.initials: CW
authors.lastname: Lam
authors.forename: Juliana C N
authors.initials: JC
authors.lastname: Chan
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D002681:China; D005260:Female; D006239:Haplotypes; D006723:Hong Kong; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000209:etiology / Q000235:genetics* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011971:Receptors, Immunologic / Q000235:genetics*; D011982:Receptors, Prostaglandin / Q000235:genetics*; D011795:Surveys and Questionnaires
chemical_list: D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; D007073:Immunoglobulin E; C036293:prostaglandin D2 receptor