v-Src-mediated transformation suppresses the expression of focal adhesion protein vinexin.
Expression of focal adhesion protein vinexin is reported to be altered in several cancer tissues; however, the mechanism of expressional change in vinexin is not known. Here we report the suppression of vinexin expression according to cellular transformation by v-Src. We found that vinexin expression was down-regulated both at the mRNA level and at the post-transcriptional level in v-Src-transformed cells. Both mTOR and MEK/ERK signals were involved in the suppression. Inhibition of these pathways by pharmacological treatment partially restored both vinexin protein and mRNA expression. Moreover, re-expression of vinexin in v-Src-transformed cells suppressed cell migration. Taken together, these observations suggest that cellular transformation by v-Src suppressed vinexin expression and that down-regulation of vinexin may be associated with oncogenic transformation.
Umemoto
Tsutomu
T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Inomoto
Takahiro
T
Ueda
Kazumitsu
K
Hamaguchi
Michinari
M
Kioka
Noriyuki
N
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002465:Cell Movement; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002472:Cell Transformation, Viral / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism*; D016391:Genes, src; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D041681:NIH 3T3 Cells; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases; D013997:Time Factors; D014162:Transfection; D048868:Adaptor Proteins, Signal Transducing
D002352:Carrier Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D009124:Muscle Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D058570:TOR Serine-Threonine Kinases; C117452:Sorbs3 protein, mouse; D048868:Adaptor Proteins, Signal Transducing; C546843:mTOR protein, mouse