title: Human mesenchymal stem cell grafts engineered to release adenosine reduce chronic seizures in a mouse model of CA3-selective epileptogenesis.
abstract: A novel generation of silk-based brain implants engineered to release adenosine was recently shown to provide robust seizure suppression in kindled rats. As a first step to develop stem cell-coated silk-based 3D-scaffolds for the therapeutic long-term delivery of adenosine we engineered human mesenchymal stem cells (hMSCs) to release adenosine. Here we demonstrate reduction of chronic seizures in a mouse model of CA3-selective epileptogenesis after infrahippocampal transplantation of adenosine-releasing hMSCs.
authors.affiliation: Robert Stone Dow Neurobiology Laboratories, Legacy Research, 1225 NE 2nd Avenue, Portland, OR 97232, USA.
authors.forename: Tianfu
authors.initials: T
authors.lastname: Li
authors.forename: Gaoying
authors.initials: G
authors.lastname: Ren
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: Detlev
authors.initials: D
authors.lastname: Boison
mesh_terms: D000241:Adenosine / Q000096:biosynthesis / Q000627:therapeutic use*; D000248:Adenosine Kinase / Q000037:antagonists & inhibitors / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000601:surgery; D006801:Humans; D007211:Indoles; D007608:Kainic Acid; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*; D023822:Tissue Engineering / Q000379:methods*; D014162:Transfection / Q000379:methods
chemical_list: D007211:Indoles; D035683:MicroRNAs; D049452:Green Fluorescent Proteins; C007293:DAPI; D000248:Adenosine Kinase; D000241:Adenosine; D007608:Kainic Acid