title: Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates.
abstract: Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
authors.affiliation: US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors.forename: Carson D
authors.initials: CD
authors.lastname: Baldwin
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Howe
authors.forename: Ranga
authors.initials: R
authors.lastname: Sampath
authors.forename: Larry B
authors.initials: LB
authors.lastname: Blyn
authors.forename: Heather
authors.initials: H
authors.lastname: Matthews
authors.forename: Vanessa
authors.initials: V
authors.lastname: Harpin
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Hall
authors.forename: Jared J
authors.initials: JJ
authors.lastname: Drader
authors.forename: Steve A
authors.initials: SA
authors.lastname: Hofstadler
authors.forename: Mark W
authors.initials: MW
authors.lastname: Eshoo
authors.forename: Karl
authors.initials: K
authors.lastname: Rudnick
authors.forename: Karen
authors.initials: K
authors.lastname: Studarus
authors.forename: David
authors.initials: D
authors.lastname: Moore
authors.forename: Sharon
authors.initials: S
authors.lastname: Abbott
authors.forename: J Michael
authors.initials: JM
authors.lastname: Janda
authors.forename: Chris A
authors.initials: CA
authors.lastname: Whitehouse
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004269:DNA, Bacterial