title: Immunohistochemical expression of matrix metalloproteinases 1, 2, and 9 in odontogenic myxoma and dental germ papilla.
abstract: The aim of this study was to compare the immunohistochemical expression of matrix metalloproteinases (MMPs) 1, 2, and 9 in odontogenic myxomas and dental germ papillae. Twelve cases of odontogenic myxoma and eight tooth germ specimens were selected for analysis of the immunohistochemical expression and the pattern of distribution of MMPs 1, 2, and 9 in extracellular matrix (ECM), as well as of the number of MMP-positive cells. MMP-2 was expressed only in the ECM of myxomas (p<0.05). No significant difference was observed between ECM immunoreactivity for MMP-9 in myxomas and dental papillae (p>0.05). MMP-1 immunoreactivity was detected in most myxoma cases at a proportion similar to that observed in dental papillae (p>0.05). A significant difference was observed in the number of immunoreactive cells in myxomas (p<0.05), MMP-1 being present at higher proportions than MMPs 2 and 9. There was a gradient in the expression of MMPs in the ECM and in neoplastic cells of odontogenic myxomas, with higher immunoreactivity to MMP-1 and lower immunoreactivity to MMP-9. Taken together, our results suggest the existence of a coordinated mechanism between MMPs 1, 2, and 9 that aimed at the efficient degradation of extracellular matrix in odontogenic myxomas.
authors.affiliation: Department of Oral Pathology, Dentistry School, Av. Senador Salgado Filho, 1787, Federal University of Rio Grande do Norte, Lagoa Nova, Natal, Rio Grande do Norte CEP 59056-000, Brazil.
authors.forename: Cassiano Francisco Weege
authors.initials: CF
authors.lastname: Nonaka
authors.forename: João
authors.initials: J
authors.lastname: Augusto Vianna Goulart Filho
authors.forename: Márcia
authors.initials: M
authors.lastname: Cristina da Costa Miguel
authors.forename: Lélia
authors.initials: L
authors.lastname: Batista de Souza
authors.forename: Leão
authors.initials: L
authors.lastname: Pereira Pinto
mesh_terms: D003771:Dental Papilla / Q000201:enzymology*; D005109:Extracellular Matrix / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000201:enzymology* / Q000473:pathology; D020781:Matrix Metalloproteinase 1 / Q000032:analysis*; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D009232:Myxoma / Q000201:enzymology* / Q000473:pathology; D009808:Odontogenic Tumors / Q000201:enzymology* / Q000473:pathology
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1