[Effects of human bone marrow mesenchymal stem cells on proliferation and apoptosis of K562 cells].
This study was aimed to compare K562 cell proliferation, cell cycle and apoptosis before and after adhesive culture with MSCs of the same and different counts, so as to investigate the effect of human bone marrow mesenchymal stem cells (MSCs) on the growth of K562 cells. Culture system of bone marrow MSCs and co-culture system of K562 cells and BMSCs in vitro were established. And K562 cell proliferation curves were drawn by co-cultured of K562 cells with different counts of BMSCs. Cell cycle and apoptosis were determined by flow cytometry. The results showed that compared with the control, the proliferation of K562 cells cultured with the same amounts of MSCs was inhibited. After co-culture with the different amounts of MSCs, MSCs exhibited a dose-dependent anti-proliferation effect on K562 cells. The percentages of K562 cells in G(1) phase and G(2) phase were higher than those of the control. It is concluded that the normal bone marrow mesenchymal stem cells can inhibit the proliferation, the progress of cell cycle and the rate of apoptosis of K562 cells. As the number of mesenchymal stem cells increased, their anti-proliferation effect on the K562 cells were enhanced in a certain range.
Ding
Ye
Y
Department of Hematology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
Lu
Hua
H
Lu
Shi-Feng
SF
Lu
Rui-Nan
RN
Liu
Peng
P
Wu
Yu-Jie
YJ
Li
Jian-Yong
JY
D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology*