title: Three dimensional digital holographic profiling of micro-fibers.
abstract: A method to measure the size, orientation, and location of opaque micro-fibers using digital holography is presented. The method involves the recording of a digital hologram followed by reconstruction at different depths. A novel combination of automated image analysis and statistical techniques, applied on the intensity of reconstructed digital holograms is used to accurately determine the characteristics of the micro-fibers. The performance of the proposed method is verified with a single fiber of known length and orientation. The potential of the method for measurement of fiber length is further demonstrated through its application to a suspension of fibers in a liquid medium.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459.
authors.forename: Michel
authors.initials: M
authors.lastname: Kempkes
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Darakis
authors.forename: Taslima
authors.initials: T
authors.lastname: Khanam
authors.forename: Arvind
authors.initials: A
authors.lastname: Rajendran
authors.forename: Vinay
authors.initials: V
authors.lastname: Kariwala
authors.forename: Marco
authors.initials: M
authors.lastname: Mazzotti
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Naughton
authors.forename: Anand K
authors.initials: AK
authors.lastname: Asundi
mesh_terms: D000465:Algorithms; D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008863:Microspheres; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity