[A quantitative method using one marker for simultaneous assay of ginsenosides in Panax ginseng and P. notoginseng].
Current quality control patterns are limited to industrial application, for most of the natural chemical reference substances are expensive and unavailable. Herein, a method, quantitative analysis of multi-components with single marker (QAMS), was established and validated to simultaneously determine nine ginsenosides (ginsenoside Rg1, Re, Rf, Rh1, Rb1, Rc, Rb2, Rb3, Rd) in P. ginseng and four ginsenosides (ginsenoside Rg1, Rh1, Rb1, Rd) in P. notoginseng. Using ginsenoside Rb1 as the contrast, the relative correction factors (RCF) of the other eight ginsenosides were determined by HPLC-DAD. Within the linear ranges, the values of RCF of ginsenoside Rb1 to ginsenoside Rg1, Re, Rf, Rh1, Rc, Rb2, Rb3 and Rd were 1.400, 1.215, 1.517, 1.801, 0.944, 1.012, 1.143, and 1.135, respectively. The RCF had a good reproducibility in various instruments, chromatographic columns (RSD = 0.30% - 3.9%). According to their RCF, we simultaneously determined nine ginsenosides in P. ginseng only using one marker. In addition, the RCF of ginsenosides were used to simultaneously quantitative analysis of four ginsenosides in P. notoginseng. The results of QAMS method were validated by comparing with that of external standard method, and no obvious significant difference was found.
Zhu
Jing-jing
JJ
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhi-min
ZM
Kuang
Yan-hui
YH
Zhang
Qi-wei
QW
Gao
Qi-pin
QP
Ma
Ni
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results
D036145:Ginsenosides; C044462:ginsenoside Rc; C048026:ginsenoside Rb2; C425564:ginsenoside Rh1; C044463:ginsenoside Rb3; C055328:ginsenoside Rf; C049863:ginsenoside Rd