title: Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.
abstract: Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.
authors.affiliation: Department of Biology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway.
authors.forename: Gyri Teien
authors.initials: GT
authors.lastname: Haugland
authors.forename: Marte
authors.initials: M
authors.lastname: Innselset
authors.forename: Dominique
authors.initials: D
authors.lastname: Madern
authors.forename: Nils-Kåre
authors.initials: NK
authors.lastname: Birkeland