title: MicroRNA profiling of clear cell renal cell cancer identifies a robust signature to define renal malignancy.
abstract: MicroRNAs are short single-stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. Only few data are available on microRNAs in clear cell renal cell carcinoma (ccRCC). We performed genome-wide expression profiling of microRNAs using microarray analysis and quantification of specific microRNAs by TaqMan real-time RT-PCR. Matched malignant and non-malignant tissue samples from two independent sets of 12 and 72 ccRCC were profiled. The microarray-based experiments identified 13 over-expressed and 20 down-regulated microRNAs in malignant samples. Expression in ccRCC tissue samples compared with matched non-malignant samples measured by RT-PCR was increased on average by 2.7- to 23-fold for the hsa-miR-16, -452*, -224, -155 and -210, but decreased by 4.8- to 138-fold for hsa-miR-200b, -363, -429, -200c, -514 and -141. No significant associations between these differentially expressed microRNAs and the clinico-pathological factors tumour stage, tumour grade and survival rate were found. Nevertheless, malignant and non-malignant tissue could clearly be differentiated by their microRNA profile. A combination of miR-141 and miR-155 resulted in a 97% overall correct classification of samples. The presented differential microRNA pattern provides a solid basis for further validation, including functional studies.
authors.affiliation: Department of Urology, Charité Universitätsmedizin, Berlin, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Jung
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Mollenkopf
authors.forename: Christina
authors.initials: C
authors.lastname: Grimm
authors.forename: Ina
authors.initials: I
authors.lastname: Wagner
authors.forename: Marco
authors.initials: M
authors.lastname: Albrecht
authors.forename: Tobias
authors.initials: T
authors.lastname: Waller
authors.forename: Christian
authors.initials: C
authors.lastname: Pilarsky
authors.forename: Manfred
authors.initials: M
authors.lastname: Johannsen
authors.forename: Carsten
authors.initials: C
authors.lastname: Stephan
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Wilfried
authors.initials: W
authors.lastname: Nietfeld
authors.forename: Thomas
authors.initials: T
authors.lastname: Rudel
authors.forename: Klaus
authors.initials: K
authors.lastname: Jung
authors.forename: Glen
authors.initials: G
authors.lastname: Kristiansen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008495:Medical Oncology / Q000379:methods; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs