Shiga toxin Stx2 production is promoted by enrofloxacin in experimental in vitro-selected mutants of Escherichia coli O157:H7 resistant to fluoroquinolones.
Enrofloxacin-resistant mutants of Stx2-producing Escherichia coli O157:H7 from cattle were selected. Mutants produced threefold higher Stx2 levels than native strains after induction with enrofloxacin. Mutants were also inducible using hundredfold higher enrofloxacin concentrations than the ones used for native strains. These results suggest that Escherichia coli O157:H7 from cattle may become more frequently pathogenic to humans as a side effect of the increasing use of veterinary fluoroquinolones.
Maurer
Claire
C
Afssa Lyon, France.
Meunier
Daniele
D
Madec
Jean-Yves
JY
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D000077422:Enrofloxacin; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D024841:Fluoroquinolones / Q000009:adverse effects* / Q000494:pharmacology; D005516:Food Microbiology; D009154:Mutation; D022641:Shiga Toxin 2 / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D022641:Shiga Toxin 2; D000077422:Enrofloxacin