title: New method development for nanoparticle extraction of water-soluble beta-(1-->3)-D-glucan from edible mushrooms, Sparassis crispa and Phellinus linteus.
abstract: Sparassis crispa and Phellinus linteus are edible/medicinal mushrooms that have remarkably high contents of beta-(1-->3)-D-glucan, which acts as a biological response modifier, but difficulty in cultivating the fruiting bodies and extraction of beta-D-glucan have restricted detailed studies. Therefore, a novel process for nanoparticle extraction of Sparan, the beta-D-glucan from Sparassis crispa, and Phellin, the beta-D-glucan from Phellinus linteus, has been investigated using insoluble tungsten carbide as a model for nanoknife technology. This is the first report showing that the nanoknife method results in high yields of Sparan (70.2%) and Phellin (65.2%) with an average particle size of 150 and 390 nm, respectively. The extracted Sparan with beta-(1-->3) linkages showed a remarkably high water solubility of 90% even after 10 min of incubation at room temperature. Therefore, it is likely that this nanoknife method could be used to produce beta-D-glucan for food, cosmetic, and pharmaceutical industries.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul, Republic of Korea.
authors.forename: Hyuk-Gu
authors.initials: HG
authors.lastname: Park
authors.forename: Youn Young
authors.initials: YY
authors.lastname: Shim
authors.forename: Seung-Oh
authors.initials: SO
authors.lastname: Choi
authors.forename: Won-Mok
authors.initials: WM
authors.lastname: Park
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001487:Basidiomycota / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D053758:Nanoparticles; D020072:Polyporales / Q000737:chemistry*; D011509:Proteoglycans; D012995:Solubility; D017973:Tungsten Compounds; D014867:Water; D047071:beta-Glucans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D011509:Proteoglycans; D017973:Tungsten Compounds; D047071:beta-Glucans; D014867:Water; C002802:tungsten carbide; C010770:polysaccharide-K