Regulation of intracellular calcium concentration by nanosecond pulsed electric fields.
Changes in [Ca(2+)](i) response of individual Jurkat cells to nanosecond pulsed electric fields (nsPEFs) of 60 ns and field strengths of 25, 50, and 100 kV/cm were investigated. The magnitude of the nsPEF-induced rise in [Ca(2+)](i) was dependent on the electric field strength. With 25 and 50 kV/cm, the [Ca(2+)](i) response was due to the release of Ca(2+) from intracellular stores and occurred in less than 18 ms. With 100 kV/cm, the increase in [Ca(2+)](i) was due to both internal release and to influx across the plasma membrane. Spontaneous changes in [Ca(2+)](i) exhibited a more gradual increase over several seconds. The initial, pulse-induced [Ca(2+)](i) response initiates at the poles of the cell with respect to electrode placement and co-localizes with the endoplasmic reticulum. The results suggest that nsPEFs target both the plasma membrane and subcellular membranes and that one of the mechanisms for Ca(2+) release may be due to nanopore formation in the endoplasmic reticulum.
Scarlett
Shaka S
SS
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.
White
Jody A
JA
Blackmore
Peter F
PF
Schoenbach
Karl H
KH
Kolb
Juergen F
JF
D000814:Aniline Compounds; D055592:Biophysical Phenomena; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002462:Cell Membrane / Q000378:metabolism; D004558:Electric Stimulation; D055724:Electrophysiological Phenomena; D005456:Fluorescent Dyes; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D019169:Jurkat Cells; D008856:Microscopy, Fluorescence; D014966:Xanthenes
D000814:Aniline Compounds; C409648:Fluo 4; D005456:Fluorescent Dyes; D014966:Xanthenes; D002118:Calcium