title: Cough reflex sensitivity is increased in the guinea pig model of allergic rhinitis.
abstract: Increased cough reflex sensitivity is found in patients with allergic rhinitis and may contribute to cough caused by rhinitis. We have reported that cough to citric acid is enhanced in the guinea pig model of allergic rhinitis. Here we address the hypothesis that the cough reflex sensitivity is increased in this model. The data from our previous studies were analyzed for the cough reflex sensitivity. The allergic inflammation in the nose was induced by repeated intranasal instillations of ovalbumin in the ovalbumin-sensitized guinea pigs. Cough was induced by inhalation of doubling concentrations of citric acid (0.05-1.6 M). Cough threshold was defined as the lowest concentration of citric acid causing two coughs (C2, expressed as geometric mean [95% confidence interval]). We found that the cough threshold was reduced in animals with allergic rhinitis. C2 was 0.5 M [0.36-0.71 M] and 0.15 M [0.1-0.23 M] prior and after repeated intranasal instillations of ovalbumin, respectively, P<0.01, n=36). C2 was not affected in control animals (n=29). We have reported that the selective leukotriene cys-LT1 receptor antagonist montelukast inhibited cough enhancement in this model. We found that this was accompanied by inhibition of the changes in cough reflex sensitivity. C2 was reduced in animals with allergic rhinitis treated orally with vehicle (0.57 M [0.28-1.1] vs. 0.09 M [0.04-0.2 M], P<0.05, n=8), but not in animals treated with montelukast (0.57 M [0.22-1.4 M] vs. 0.52 M [0.17-1.6 M], NS, n=8). We conclude that the cough reflex sensitivity is increased in the guinea pig model of allergic rhinitis. Our results suggest that guinea pig is a suitable model for mechanistic studies of increased cough reflex sensitivity in rhinitis.
authors.affiliation: Department of Pathophysiology, Jessenius School of Medicine, Comenius University, Martin, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Brozmanova
authors.forename: J
authors.initials: J
authors.lastname: Plevkova
authors.forename: M
authors.initials: M
authors.lastname: Tatar
authors.forename: M
authors.initials: M
authors.lastname: Kollarik
mesh_terms: D000085:Acetates / Q000627:therapeutic use; D000485:Allergens / Q000494:pharmacology; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D019343:Citric Acid; D003371:Cough / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology*; D003521:Cyclopropanes; D006168:Guinea Pigs; D007249:Inflammation / Q000473:pathology; D020024:Leukotriene Antagonists / Q000627:therapeutic use; D008297:Male; D009297:Nasal Mucosa / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D011804:Quinolines / Q000627:therapeutic use; D012018:Reflex / Q000187:drug effects / Q000502:physiology*; D006255:Rhinitis, Allergic, Seasonal / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D013440:Sulfides
chemical_list: D000085:Acetates; D000485:Allergens; D018927:Anti-Asthmatic Agents; D003521:Cyclopropanes; D020024:Leukotriene Antagonists; D011804:Quinolines; D013440:Sulfides; D019343:Citric Acid; D010047:Ovalbumin; C093875:montelukast