Multidetermination of four nitrofurans in animal feeds by a sensitive and simple enzyme-linked immunosorbent assay.
In this study, the polyclonal antibody against furazolidone was produced with furazolidone coupling to protein carriers by a diazotization method and glutaraldehyde reaction, respectively. The antibody obtained showed good specificity toward furazolidone and various cross-reactivity toward nitrofurantoin, nitrofurazone, and furaltadone. Then, an indirect competitive enzyme-linked immunosorbent assay (ELISA) based on the antibody was first developed for multidetermination of four nitrofurans in animal feeds. The limit of detection (LOD) of the method was 0.2-2.1 ng/g depending on the component. After simple extraction, the fortified swine and broiler chicken feed samples were detected with recovery ranges of 75.9-86.4%. Results obtained from ELISA were confirmed by high-performance liquid chromatography (HPLC) with ultraviolet detection. Analysis of the unknown feed samples indicates that ELISA can be a practical tool for screening of nitrofurans in animal feeds before confirmation by HPLC.
Li
Jun
J
College of Animal Science & Technology, Agricultural University of Hebei, Baoding Hebei, China.
Liu
Ju Xiang
JX
Wang
Jian Ping
JP
D000821:Animal Feed / Q000032:analysis*; D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009581:Nitrofurans / Q000032:analysis*; D012680:Sensitivity and Specificity; D019155:Veterinary Drugs / Q000032:analysis
D000890:Anti-Infective Agents; D009581:Nitrofurans; D019155:Veterinary Drugs