Coordinated implementation of chikungunya virus reverse transcription-PCR.
A preformulated chikungunya virus real-time reverse transcription-PCR, quality-confirmed oligonucleotides, and noninfectious virus controls were distributed by the European Network for the Diagnosis of Imported Viral Diseases. An international proficiency study with 31 participants demonstrated that ad hoc implementation of molecular diagnostics was feasible and successful.
Panning
Marcus
M
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Charrel
Remi N
RN
Donoso Mantke
Oliver
O
Mantke
Oliver D
OD
Landt
Olfert
O
Niedrig
Matthias
M
Drosten
Christian
C
D018354:Alphavirus Infections / Q000175:diagnosis* / Q000821:virology; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005060:Europe; D006801:Humans; D011786:Quality Control; D012367:RNA, Viral / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic