title: Cytokinin regulates root meristem activity via modulation of the polar auxin transport.
abstract: Plant development is governed by signaling molecules called phytohormones. Typically, in certain developmental processes more than 1 hormone is implicated and, thus, coordination of their overlapping activities is crucial for correct plant development. However, molecular mechanisms underlying the hormonal crosstalk are only poorly understood. Multiple hormones including cytokinin and auxin have been implicated in the regulation of root development. Here we dissect the roles of cytokinin in modulating growth of the primary root. We show that cytokinin effect on root elongation occurs through ethylene signaling whereas cytokinin effect on the root meristem size involves ethylene-independent modulation of transport-dependent asymmetric auxin distribution. Exogenous or endogenous modification of cytokinin levels and cytokinin signaling lead to specific changes in transcription of several auxin efflux carrier genes from the PIN family having a direct impact on auxin efflux from cultured cells and on auxin distribution in the root apex. We propose a novel model for cytokinin action in regulating root growth: Cytokinin influences cell-to-cell auxin transport by modification of expression of several auxin transport components and thus modulates auxin distribution important for regulation of activity and size of the root meristem.
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium.
authors.forename: Kamil
authors.initials: K
authors.lastname: Ruzicka
authors.forename: Mária
authors.initials: M
authors.lastname: Simásková
authors.forename: Jerome
authors.initials: J
authors.lastname: Duclercq
authors.forename: Jan
authors.initials: J
authors.lastname: Petrásek
authors.forename: Eva
authors.initials: E
authors.lastname: Zazímalová
authors.forename: Sibu
authors.initials: S
authors.lastname: Simon
authors.forename: Jirí
authors.initials: J
authors.lastname: Friml
authors.forename: Marc C E
authors.initials: MC
authors.lastname: Van Montagu
authors.forename: Eva
authors.initials: E
authors.lastname: Benková
mesh_terms: D001692:Biological Transport; D003583:Cytokinins / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D018519:Meristem / Q000502:physiology*; D010937:Plant Growth Regulators; D018521:Plant Physiological Phenomena; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development*
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators