A single U/C nucleotide substitution changing alanine to valine in the beet necrotic yellow vein virus P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings.
Beet necrotic yellow vein virus (BNYVV) A type isolates E12 and S8, originating from areas where resistance-breaking had or had not been observed, respectively, served as starting material for studying the influence of sequence variations in BNYVV RNA 3 on virus accumulation in partially resistant sugar beet varieties. Sub-isolates containing only RNAs 1 and 2 were obtained by serial local lesion passages; biologically active cDNA clones were prepared for RNAs 3 which differed in their coding sequences for P25 aa 67, 68 and 129. Sugar beet seedlings were mechanically inoculated with RNA 1+2/RNA 3 pseudorecombinants. The origin of RNAs 1+2 had little influence on virus accumulation in rootlets. E12 RNA 3 coding for V(67)C(68)Y(129) P25, however, enabled a much higher virus accumulation than S8 RNA 3 coding for A(67)H(68)H(129) P25. Mutants revealed that this was due only to the V(67) 'GUU' codon as opposed to the A(67) 'GCU' codon.
Koenig
R
R
Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
Loss
S
S
Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
Specht
J
J
Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
Varrelmann
M
M
Institut für Zuckerrübenforschung, Abteilung Phytomedizin, Holtenser Landstraße 77, D-37079 Göttingen, Germany.
Lüddecke
P
P
Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
Deml
G
G
Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, D-38106 Braunschweig, Germany.
D000409:Alanine / Q000737:chemistry; D019943:Amino Acid Substitution; D027461:Beta vulgaris / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018517:Plant Roots / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000821:virology*; D017422:Sequence Analysis, DNA; D014633:Valine / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D012329:RNA, Bacterial; D012367:RNA, Viral; C096035:RNAIII, Staphylococcus aureus; D014764:Viral Proteins; D014633:Valine; D000409:Alanine