[Angiotensin II increases ROS production in cardiac fibroblasts by inducing p22phox over-expression].
OBJECTIVE
To investigate the changes in the reactive oxygen species (ROS) in rat cardiac fibroblasts exposed to angiotensin II (Ang II) treatment and explore the possible pathways that mediate ROS production.


METHODS
In vitro cultured fetal rat cardiac fibroblasts treated with apocynin (APO, 100 micromol/L), Ang II (10(-7) mol/L), or APO+Ang II (10(-7) mol/L Ang II was added 1 h after 100 micromol/L APO), and the ROS levels and p22phox expression in the cells were detected using fluorescent microscope and immunohistochemistry, respectively.


RESULTS
Compared with the normal control cells, Ang II treatment of the cardiac fibroblasts resulted in significantly increased ROS production, the effect of which was inhibited by the application of APO. p22phox expression was hardly detected by immunohistochemistry in the control cells, but over-expressed in AngII-treated cells. APO substantially decreased the over-expression of p22phox induced by Ang II.


CONCLUSIONS
Ang II increases ROS production in fetal rat cardiac fibroblasts probably by inducing p22phox over-expression.
Guo
Rong
R
Department of Physiology and Pathophysiology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. guoguo1616@sina.com
Zhou
Juan
J
Deng
Xiu-ling
XL
Gao
Guang-dao
GD
Jiang
Xiao-ying
XY
Lin
Yuan-xi
YX
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology*; D019255:NADPH Oxidases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
D017382:Reactive Oxygen Species; D000804:Angiotensin II; D019255:NADPH Oxidases; C515768:Cyba protein, rat