title: New approach to study of T cellular immunity development: parallel investigation of lymphoid organ formation and changes in immune proteasome amount in rat early ontogenesis.
abstract: The expression pattern and distribution of proteasome immune subunits LMP7 and LMP2 in the developing rat spleen and liver as well as the periarterial lymphoid sheath formation were investigated. LMP7 and LMP2 were detected by immunoblotting in the spleen on the 21st embryonic day and during the first postnatal days in equal amounts. Their levels increased by the 8th and 18th postnatal days. Double immunofluorescent labeling the spleen cells revealed LMP7 and LMP2 in T and B lymphocytes localized in the red pulp in embryogenesis. Few T lymphocytes were discovered in periarterial zones on the 8th postnatal day. T lymphocytes filled these zones and formed lymphoid sheaths by the 18-19th day. In the liver, LMP7 and LMP2 were revealed by the 17-19th postnatal day. Immunofluorescent analysis showed their presence in hepatocytes at this period. The data suggest that T cell-mediated immune response in relation to hepatocytes is possible beginning from 18th to 19th postnatal day.
authors.affiliation: N.K. Koltsov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia. npsharova@bk.ru
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Sharova
authors.forename: Liudmila A
authors.initials: LA
authors.lastname: Zakharova
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Astakhova
authors.forename: Yaroslava D
authors.initials: YD
authors.lastname: Karpova
authors.forename: Victoria I
authors.initials: VI
authors.lastname: Melnikova
authors.forename: Svetlana B
authors.initials: SB
authors.lastname: Dmitrieva
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Lyupina
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Erokhov
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000276:immunology* / Q000378:metabolism*; D047108:Embryonic Development / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D008099:Liver / Q000196:embryology / Q000201:enzymology / Q000254:growth & development / Q000276:immunology; D008221:Lymphoid Tissue / Q000196:embryology* / Q000201:enzymology / Q000254:growth & development / Q000276:immunology*; D009097:Multienzyme Complexes / Q000276:immunology* / Q000378:metabolism*; D011247:Pregnancy; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000196:embryology / Q000201:enzymology / Q000254:growth & development / Q000276:immunology; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology*; D014018:Tissue Distribution
chemical_list: D009097:Multienzyme Complexes; C071313:LMP-2 protein; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex