title: E. coli expression and purification of Fab antibody fragments.
abstract: The Fab molecule was the first generated antibody fragment and still dominates basic research and clinical applications. This unit describes the E. coli expression and purification of Fab antibody fragments with and without a His tag, and is designed to yield sufficient protein for the evaluation and characterization of a panel of Fab selected from a Fab library by phage display.
authors.affiliation: Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Ka Yin
authors.initials: KY
authors.lastname: Kwong
authors.forename: Christoph
authors.initials: C
authors.lastname: Rader
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D006041:Goats; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification*; D009842:Oligopeptides / Q000737:chemistry; D019151:Peptide Library
chemical_list: C471213:His-His-His-His-His-His; D007140:Immunoglobulin Fab Fragments; D009842:Oligopeptides; D019151:Peptide Library; D006639:Histidine