title: Human and murine amniotic fluid c-Kit+Lin- cells display hematopoietic activity.
abstract: We have isolated c-Kit(+)Lin(-) cells from both human and murine amniotic fluid (AF) and investigated their hematopoietic potential. In vitro, the c-Kit(+)Lin(-) population in both species displayed a multilineage hematopoietic potential, as demonstrated by the generation of erythroid, myeloid, and lymphoid cells. In vivo, cells belonging to all 3 hematopoietic lineages were found after primary and secondary transplantation of murine c-Kit(+)Lin(-) cells into immunocompromised hosts, thus demonstrating the ability of these cells to self-renew. Gene expression analysis of c-Kit(+) cells isolated from murine AF confirmed these results. The presence of cells with similar characteristics in the surrounding amnion indicates the possible origin of AF c-Kit(+)Lin(-) cells. This is the first report showing that cells isolated from the AF do have hematopoietic potential; our results support the idea that AF may be a new source of stem cells for therapeutic applications.
authors.affiliation: Inserm U768, Paris, France.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ditadi
authors.forename: Paolo
authors.initials: P
authors.lastname: de Coppi
authors.forename: Olivier
authors.initials: O
authors.lastname: Picone
authors.forename: Laetitia
authors.initials: L
authors.lastname: Gautreau
authors.forename: Rim
authors.initials: R
authors.lastname: Smati
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Six
authors.forename: Delphine
authors.initials: D
authors.lastname: Bonhomme
authors.forename: Sophie
authors.initials: S
authors.lastname: Ezine
authors.forename: René
authors.initials: R
authors.lastname: Frydman
authors.forename: Marina
authors.initials: M
authors.lastname: Cavazzana-Calvo
authors.forename: Isabelle
authors.initials: I
authors.lastname: André-Schmutz
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism*; D000818:Animals; D019070:Cell Lineage; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006410:Hematopoiesis; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; C064658:RAG-1 protein; D019009:Proto-Oncogene Proteins c-kit