title: PKCalpha tumor suppression in the intestine is associated with transcriptional and translational inhibition of cyclin D1.
abstract: Alterations in PKC isozyme expression and aberrant induction of cyclin D1 are early events in intestinal tumorigenesis. Previous studies have identified cyclin D1 as a major target in the antiproliferative effects of PKCalpha in non-transformed intestinal cells; however, a link between PKC signaling and cyclin D1 in colon cancer remained to be established. The current study further characterized PKC isozyme expression in intestinal neoplasms and explored the consequences of restoring PKCalpha or PKCdelta in a panel of colon carcinoma cell lines. Consistent with patterns of PKC expression in primary tumors, PKCalpha and delta levels were generally reduced in colon carcinoma cell lines, PKCbetaII was elevated and PKCepsilon showed variable expression, thus establishing the suitability of these models for analysis of PKC signaling. While colon cancer cells were insensitive to the effects of PKC agonists on cyclin D1 levels, restoration of PKCalpha downregulated cyclin D1 by two independent mechanisms. PKCalpha expression consistently (a) reduced steady-state levels of cyclin D1 by a novel transcriptional mechanism not previously seen in non-transformed cells, and (b) re-established the ability of PKC agonists to activate the translational repressor 4E-BP1 and inhibit cyclin D1 translation. In contrast, PKCdelta had modest and variable effects on cyclin D1 steady-state levels and failed to restore responsiveness to PKC agonists. Notably, PKCalpha expression blocked anchorage-independent growth in colon cancer cells via a mechanism partially dependent on cyclin D1 deficiency, while PKCdelta had only minor effects. Loss of PKCalpha and effects of its re-expression were independent of the status of the APC/beta-catenin signaling pathway or known genetic alterations, indicating that they are a general characteristic of colon tumors. Thus, PKCalpha is a potent negative regulator of cyclin D1 expression and anchorage-independent cell growth in colon tumor cells, findings that offer important perspectives on the frequent loss of this isozyme during intestinal carcinogenesis.
authors.affiliation: Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York 14263, USA.
authors.forename: Marybeth A
authors.initials: MA
authors.lastname: Pysz
authors.forename: Olga V
authors.initials: OV
authors.lastname: Leontieva
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Bateman
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Uronis
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Curry
authors.forename: David W
authors.initials: DW
authors.lastname: Threadgill
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Janssen
authors.forename: Sylvie
authors.initials: S
authors.lastname: Robine
authors.forename: Anna
authors.initials: A
authors.lastname: Velcich
authors.forename: Leonard H
authors.initials: LH
authors.lastname: Augenlicht
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Black
authors.forename: Jennifer D
authors.initials: JD
authors.lastname: Black
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007414:Intestinal Neoplasms / Q000503:physiopathology*; D051379:Mice; D011401:Promoter Regions, Genetic / Q000187:drug effects; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D019938:Cyclin D1; D011493:Protein Kinase C