title: Role of serum amyloid a in adipocyte-macrophage cross talk and adipocyte cholesterol efflux.
abstract: BACKGROUND
  Acute phase serum amyloid A (A-SAA) is secreted by hepatocytes in response to injury and is regulated by proinflammatory cytokines. In obese humans, adipocytes are also a major contributor to circulating A-SAA levels.
  
  
  OBJECTIVE
  We aimed to investigate the role and regulation of A-SAA in human adipose tissue (AT).
  
  
  METHODS
  An approach combining microarrays and the FunNet bioinformatics tool was applied to human AT fractions (i.e. adipocytes vs. stroma vascular fraction) to hypothesize genes and functions related to A-SAA. Experiments with human AT from 37 obese subjects and human multipotent adipose-derived stem (hMADS) cells were used to confirm the microarray driven hypotheses.
  
  
  RESULTS
  Microarray analysis highlighted the relationship between A-SAA and stroma vascular fraction inflammatory genes, and between A-SAA and adipocyte-expressed ATP-binding cassette (ABC) transporters. We confirmed that serum amyloid A (SAA) protein is expressed in sc AT of obese subjects (n = 37, body mass index = 49.3 +/- 1.5 kg/m(2)) and showed that SAA protein expression correlated with adipocyte size (R = 0.44; P = 6.10(-3)), macrophage infiltration (R = 0.61; P = 10(-4)), and ABC subfamily A1 protein expression (R = 0.43; P = 9.10(-3)). IL-1beta, TNF-alpha, and human AT macrophage-conditioned medium significantly induced A-SAA secretion (from 2.6 to 7.6 fold) in hMADS cells. Recombinant SAA induced cholesterol ABC subfamily A1-dependent efflux from hMADS adipocytes by 4.3-fold in a dose-dependent manner.
  
  
  CONCLUSIONS
  This work provides original insight suggesting that A-SAA is a player in the dialogue between hypertrophied adipocytes and macrophages through its regulation of adipocyte cholesterol efflux.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, U872 team7, Nutriomique, Cordelier Research Center, Paris 75006, France. christine.poitou-bernert@psl.aphp.fr
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Adeline
authors.initials: A
authors.lastname: Divoux
authors.forename: Aurélie
authors.initials: A
authors.lastname: Faty
authors.forename: Joan
authors.initials: J
authors.lastname: Tordjman
authors.forename: Danielle
authors.initials: D
authors.lastname: Hugol
authors.forename: Abdelhaim
authors.initials: A
authors.lastname: Aissat
authors.forename: Mayoura
authors.initials: M
authors.lastname: Keophiphath
authors.forename: Corneliu
authors.initials: C
authors.lastname: Henegar
authors.forename: Stéphane
authors.initials: S
authors.lastname: Commans
authors.forename: Karine
authors.initials: K
authors.lastname: Clément
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000502:physiology*; D052436:Adipose Tissue, White / Q000166:cytology / Q000378:metabolism; D000328:Adult; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation; D002784:Cholesterol / Q000378:metabolism*; D003470:Culture Media; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism* / Q000502:physiology*; D008297:Male; D009767:Obesity, Morbid / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000685:Serum Amyloid A Protein / Q000502:physiology*
chemical_list: D003470:Culture Media; D017077:Culture Media, Conditioned; D000685:Serum Amyloid A Protein; D002784:Cholesterol