Modulation of deoxysugar transfer by the elloramycin glycosyltransferase ElmGT through site-directed mutagenesis.
The glycosyltransferase ElmGT from Streptomyces olivaceus is involved in the biosynthesis of the antitumor drug elloramycin, and it has been shown to possess a broad deoxysugar recognition pattern, being able to transfer different l- and d-deoxysugars to 8-demethyl-tetracenomycin C, the elloramycin aglycone. Site-directed mutagenesis in residues L309 and N312, located in the alpha/beta/alpha motif within the nucleoside diphosphate-sugar binding region, can be used to modulate the substrate flexibility of ElmGT, making it more precise for transfer of specific deoxysugars.
Ramos
Angelina
A
Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.
Olano
Carlos
C
Braña
Alfredo F
AF
Méndez
Carmen
C
Salas
José A
JA
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003837:Deoxy Sugars / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D003837:Deoxy Sugars; D016695:Glycosyltransferases; C435165:Elloramycin glycosyltransferase, Streptomyces olivaceus