title: Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment.
abstract: A Gram-positive, aerobic, non-motile actinobacterium, designated strain Ms392A(T), was isolated from an ocean-sediment sample collected from the South China Sea. The isolate contained chemical markers that supported chemotaxonomic assignment to the genus Amycolatopsis. On the basis of an analysis of 16S rRNA gene sequence similarities, strain Ms392A(T) represents a novel subclade within the genus Amycolatopsis, with Amycolatopsis palatopharyngis 1BDZ(T) as its closest phylogenetic neighbour (99.4 % similarity). However, DNA-DNA hybridization demonstrated that strain Ms392A(T) was distinct from A. palatopharyngis AS 4.1729(T) (48.6 % relatedness). The polyphasic analysis demonstrated that the ocean isolate can be clearly distinguished from recognized species of the genus Amycolatopsis. Therefore, strain Ms392A(T) represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis marina sp. nov. is proposed. The type strain is Ms392A(T) (=CGMCC 4.3568(T) =NBRC 104263(T)).
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Bian
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Fu-Hang
authors.initials: FH
authors.lastname: Song
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Huan-Qin
authors.initials: HQ
authors.lastname: Dai
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Xinling
authors.initials: X
authors.lastname: Hu
authors.forename: Zhi-Heng
authors.initials: ZH
authors.lastname: Liu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S