A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source.
X-ray crystallography at third-generation synchrotron sources permits tremendous insight into the three-dimensional structure of macromolecules. Additional information is, however, often required to aid the transition from structure to function. In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes. Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source. The continuing development of the spectrometer is also outlined.
Owen
Robin L
RL
Swiss Light Source, Paul Scherrer Institute, CH-5232 Villigen PSI, Switzerland.
Pearson
Arwen R
AR
Meents
Alke
A
Boehler
Pirmin
P
Thominet
Vincent
V
Schulze-Briese
Clemens
C
D017076:Computer-Aided Design; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046911:Macromolecular Substances / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation* / Q000379:methods
D046911:Macromolecular Substances