title: Antineoplastic agents. 570. Isolation and structure elucidation of bacillistatins 1 and 2 from a marine Bacillus silvestris.
abstract: Two new cyclodepsipeptides designated bacillistatins 1 (1) and 2 (2) have been isolated from cultures of a sample of Bacillus silvestris that was obtained from a Pacific Ocean (southern Chile) crab. Each 12-unit cyclodepsipeptide strongly inhibited growth of a human cancer cell line panel, with GI(50)'s of 10(-4)-10(-5) microg/mL, and each compound was active against antibiotic-resistant Streptococcus pneumoniae. The structures were elucidated by a combination of X-ray diffraction and mass and 2D NMR spectroscopic analyses, together with chemical degradation.
authors.affiliation: Cancer Research Institute and Department of Chemistry and Biochemistry, Arizona State University, P.O. Box 871604, Tempe, Arizona 85287-1604, USA. bpettit@asu.edu
authors.forename: George R
authors.initials: GR
authors.lastname: Pettit
authors.forename: John C
authors.initials: JC
authors.lastname: Knight
authors.forename: Delbert L
authors.initials: DL
authors.lastname: Herald
authors.forename: Robin K
authors.initials: RK
authors.lastname: Pettit
authors.forename: Fiona
authors.initials: F
authors.lastname: Hogan
authors.forename: Venugopal J R V
authors.initials: VJ
authors.lastname: Mukku
authors.forename: John S
authors.initials: JS
authors.lastname: Hamblin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dodson
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Chapuis
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry*; D018360:Crystallography, X-Ray; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D008297:Male; D008386:Marine Biology; D051379:Mice; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D047630:Depsipeptides; C548569:bacillistatin 1; C548570:bacillistatin 2