Differential expression of sigH paralogs during growth and under different stress conditions in Mycobacterium smegmatis.
SigH regulates a transcriptional network that responds to heat and oxidative stress in mycobacteria. Seven sigH paralogs are reported to exist in the Mycobacterium smegmatis genome. A comprehensive real-time reverse transcriptase PCR analysis during different stages of growth and upon exposure to various stress conditions and antimycobacterial compounds showed differential expression of sigH paralogs during stationary phase and severalfold increases in the levels of transcription of sigH1, sigH4, sigH5, sigH6, and sigH7 under specific stress conditions.
Singh
Anirudh K
AK
Division of Microbiology, Central Drug Research Institute, Lucknow 226 001, India. bhupendransingh@gmail.com
Singh
Bhupendra N
BN
D000995:Antitubercular Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D005798:Genes, Bacterial; D006358:Hot Temperature; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018384:Oxidative Stress; D020033:Protein Isoforms / Q000096:biosynthesis*; D012808:Sigma Factor / Q000096:biosynthesis*
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D020033:Protein Isoforms; C120328:SigH protein, bacteria; D012808:Sigma Factor