title: Analysis of the poplar phloem proteome and its response to leaf wounding.
abstract: Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protein. This is the first characterization of a phloem proteome from a tree species.
authors.affiliation: Centre for Forest Biology and Department of Biology, University of Victoria, Victoria, BC, Canada.
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: Dafoe
authors.forename: Arezoo
authors.initials: A
authors.lastname: Zamani
authors.forename: Abul K M
authors.initials: AK
authors.lastname: Ekramoddoullah
authors.forename: Dustin
authors.initials: D
authors.lastname: Lippert
authors.forename: Jörg
authors.initials: J
authors.lastname: Bohlmann
authors.forename: C Peter
authors.initials: CP
authors.lastname: Constabel
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D052585:Phloem / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013314:Stress, Mechanical; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D020543:Proteome