title: Effect of an oil byproduct from conjugated linoleic acid (CLA) purification on CLA accumulation and lipogenic gene expression in broilers.
abstract: A previous study showed that supplementing broilers with an oil byproduct obtained during the purification process of conjugated linoleic acid (CLA) from safflower oil could result in CLA-enriched egg yolks more efficiently than feeding purified CLA (free fatty acid form). On this basis, this study evaluated whether dietary CLA byproduct (CBP) supplementation would enhance CLA accumulation in broiler muscle and its lipogenic mRNA expression in the liver. A total of 456 1-day-old male broiler chicks were randomly assigned to four groups, each of which was given one of the following 2% dietary supplements for 4 weeks: soybean oil (control), safflower oil (SAF), purified CLA, and CBP. During the feeding trial, little alteration in broiler performance was observed among the test groups. CLA accumulation efficiency in the breast muscle did not differ significantly between the CLA- and CBP-fed groups after feeding of the test diet for 3 weeks. CLA supplementation also induced lipogenesis in the livers of the broilers, and it significantly increased the relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), as well as its target genes: fatty acid synthase (FAS) and acetyl coenzyme A carboxylase (ACC) (p < 0.05). However, in the CBP-fed group, SREBP1 and ACC mRNA levels were not significantly different from the controls (p > 0.05). These results suggest that CBP could be an efficient dietary source that promotes CLA accumulation in broiler muscle without inducing lipogenesis in the liver or compromising performance and meat quality in the birds.
authors.affiliation: Department of Food and Biotechnology, Korea University, Chungnam, Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Wan Sik
authors.initials: WS
authors.lastname: Jeong
authors.forename: In Ho
authors.initials: IH
authors.lastname: Kim
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Geun Ho
authors.initials: GH
authors.lastname: Kang
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Ho Geun
authors.initials: HG
authors.lastname: Yoon
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Ham
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D019587:Dietary Supplements; D015870:Gene Expression / Q000187:drug effects*; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000302:isolation & purification / Q000378:metabolism*; D050155:Lipogenesis / Q000235:genetics*; D008099:Liver / Q000737:chemistry; D008297:Male; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D012450:Safflower Oil / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry
chemical_list: D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D012333:RNA, Messenger; D013024:Soybean Oil; D012450:Safflower Oil