Induction of growth phase-specific autolysis in Bacillus subtilis 168 by growth inhibitors.
Growth phase-specific autolysis of Bacillus subtilis by inhibitors of membrane permeability, inhibitors of macromolecule biosynthesis, inhibitors of cell wall biosynthesis and detergents were tested and characterized in glucose limited liquid medium. The minimum autolysin induction concentration (MAIC) of test compounds, which was at least 1/20th lower than the conventional autolysis induction concentration, induced autolysis only for cells at the glucose exhaustion point (diauxic point) of the growth phase, while it was not induced for cells at pre- and post-diauxic points. Inhibitors of macromolecule synthesis that are not known for inducing autolysis, such as chloramphenicol, rifampicin, nalidixic acid, and detergents, also induced specific autolysis. Two types of autolysis corresponding to the concentrations of compounds are distinguished: concentration-sensitive and concentration-insensitive types.
Chung
Jin-Kyo
JK
Department of Physiology, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.
Yoon
Hyun Ee
HE
Shin
Ha Chul
HC
Choi
Eun-Young
EY
Byeon
Woo-Hyeon
WH
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D001433:Bacteriolysis / Q000187:drug effects*; D002473:Cell Wall / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D019810:Sodium Azide / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D006131:Growth Inhibitors; D007476:Ionophores; D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D013501:Surface-Active Agents; D003609:Dactinomycin; D012967:Sodium Dodecyl Sulfate; D002701:Chloramphenicol; D000667:Ampicillin; D019810:Sodium Azide; D009238:N-Acetylmuramoyl-L-alanine Amidase; D005947:Glucose; D012293:Rifampin