Glyoxalase I activity and immunoreactivity in the aging  human lens.
Glyoxalase I (GLOI) is the first enzyme of the glyoxalase system that catalyzes the metabolism of reactive dicarbonyls, such as methylglyoxal (MGO). During aging and cataract development, human lens proteins are chemically modified by MGO, which is likely due to inadequate metabolism of MGO by the glyoxalase system. In this study, we have determined the effect of aging on GLOI activity and the immunoreactivity and morphological distribution of GLOI in the human lens. A monoclonal antibody was developed against human GLOI. GLOI immunoreactivity was strongest in the anterior epithelial cells and weaker in rest of the lens. Cultured human lens epithelial cells showed immunostaining throughout the cytoplasm. In the human lens, GLOI activity and immunoreactivity both decreased with age. We believe that this would lead to promotion of MGO-modification in aging lens proteins.
Mailankot
Maneesh
M
Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Pathology Building 311, 2085 Adelbert Road, Cleveland, OH 44106, USA.
Padmanabha
Smitha
S
Pasupuleti
NagaRekha
N
Major
Denice
D
Howell
Scott
S
Nagaraj
Ram H
RH
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D002478:Cells, Cultured; D016922:Cellular Senescence; D002648:Child; D003001:Cloning, Molecular; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry; D007791:Lactoylglutathione Lyase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology / Q000201:enzymology*; D008875:Middle Aged; D055815:Young Adult
D000911:Antibodies, Monoclonal; D007791:Lactoylglutathione Lyase