Proteomic profiling in apolipoprotein E-deficient mice during atherosclerosis progression.
Atherosclerosis and related complications are a major worldwide cause of human morbidity and mortality. It is advantageous to perform atherosclerosis studies in the apolipoprotein E-deficient (Apo E(-/-)) mouse model, which develops atherosclerosis very fast in comparison to humans. The aim of this study was to compare serum protein profiles in Apo E(-/-) mice during atherosclerosis progression with the data of control C57BL/6 mice. Serum proteomic analyses were performed using surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS). The protein profiles obtained using three different chips, CM-10 (weak cation exchange), H50 (reversed-phase) and IMAC-30 (immobilized metal affinity capture) were statistically analyzed using the ProteinChip data manager 3.0 program. At 20 weeks of age, all Apo E(-/-) mice developed early atherosclerotic lesions. The peak intensities of 742 serum protein/peptide clusters were found to be different between the atherosclerotic and control mice groups, and the differences reached statistical significance for 107 serum protein/peptide clusters (p<0.05). This study contributes to understanding the changes in serum protein/peptide profiles during atherosclerosis development and may inform discovery of new protein biomarkers for early diagnosis of atherosclerosis.
Dursun
Evrim
E
Medical Faculty, Department of Biochemistry, Akdeniz University, 07070 Antalya, Turkey.
Ozben
Beste
B
Monari
Emanuela
E
Cuoghi
Aurora
A
Tomasi
Aldo
A
Ozben
Tomris
T
D000818:Animals; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D050197:Atherosclerosis / Q000097:blood* / Q000503:physiopathology; D004195:Disease Models, Animal; D051379:Mice; D018345:Mice, Knockout; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001057:Apolipoproteins E