title: Plasma levels of heat shock protein 72 (HSP72) and beta-endorphin as indicators of stress, pain and prognosis in horses with colic.
abstract: A prospective observational study was performed to evaluate whether the plasma concentration of heat shock protein 72 (HSP72) or beta-endorphin is related to clinical signs, blood chemistry, or severity of pain of colic. Seventy-seven horses with colic and 15 clinically healthy controls were studied. The horses were divided into four groups which reflected increasing severity of colic, from normal control horses to horses with mild, moderate and severe colic. Blood samples were collected before any treatment. Packed cell volume (PCV) and plasma HSP72, beta-endorphin, cortisol, adrenocorticotropic hormone (ACTH) and lactate concentrations were measured. Plasma beta-endorphin was related with severity of colic and survival, as well as with plasma cortisol, ACTH and lactate concentrations, heart rate, PCV and pain score. High plasma HSP72 concentration may indicate circulatory deficits, but was not associated with clinical signs of colic. Plasma lactate still seemed to be the most useful single prognostic parameter in horses with colic.
authors.affiliation: University of Helsinki, Faculty of Veterinary Medicine, Department of Equine and Small Animal Medicine, P.O. Box 57, 00014 Helsinki, Finland. kati.niinisto@fimnet.fi
authors.forename: Kati E
authors.initials: KE
authors.lastname: Niinistö
authors.forename: Raija V
authors.initials: RV
authors.lastname: Korolainen
authors.forename: Marja R
authors.initials: MR
authors.lastname: Raekallio
authors.forename: Anna K
authors.initials: AK
authors.lastname: Mykkänen
authors.forename: Ninna M
authors.initials: NM
authors.lastname: Koho
authors.forename: Mirja O
authors.initials: MO
authors.lastname: Ruohoniemi
authors.forename: Juhani
authors.initials: J
authors.lastname: Leppäluoto
authors.forename: A Reeta
authors.initials: AR
authors.lastname: Pösö
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000662:veterinary; D016022:Case-Control Studies; D003085:Colic / Q000097:blood / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D005260:Female; D050884:HSP72 Heat-Shock Proteins / Q000097:blood*; D006734:Horse Diseases / Q000097:blood* / Q000401:mortality / Q000473:pathology; D006736:Horses; D006854:Hydrocortisone / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008297:Male; D010146:Pain / Q000097:blood / Q000662:veterinary; D011379:Prognosis; D011446:Prospective Studies; D012720:Severity of Illness Index; D001615:beta-Endorphin / Q000097:blood*
chemical_list: D015415:Biomarkers; D050884:HSP72 Heat-Shock Proteins; D019344:Lactic Acid; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone