title: Alginate molecular mass produced by Azotobacter vinelandii in response to changes of the O2 transfer rate in chemostat cultures.
abstract: Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g l(-1)) was observed. At a constant growth rate (0.1 h(-1)), the mean molecular mass of the alginate was strongly influenced by changes in the OTR, varying from 860 to 1,690 kDa. These results support a possible relationship between alginate polymerization-depolymerization process and the O(2) uptake rate.
authors.affiliation: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147, Casilla, 4059 Valparaiso, Chile. alvaro.diaz@ucv.cl
authors.forename: A
authors.initials: A
authors.lastname: Díaz-Barrera
authors.forename: P
authors.initials: P
authors.lastname: Silva
authors.forename: R
authors.initials: R
authors.lastname: Avalos
authors.forename: F
authors.initials: F
authors.lastname: Acevedo
mesh_terms: D000464:Alginates / Q000378:metabolism*; D016948:Azotobacter vinelandii / Q000378:metabolism*; D018533:Biomass; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D010100:Oxygen