title: Expression and regulation of cholesterol sulfotransferase (SULT2B1b) in human endometrium.
abstract: OBJECTIVE
  To investigate the hormonal regulation of SULT2B1b in human endometrium.
  
  
  METHODS
  In vitro study with human endometrial tissues and cultured human endometrial cells.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Thirty-seven women undergoing hysterectomy for benign disease.
  
  
  METHODS
  Human endometrial tissues were collected for in situ hybridization. Culture medium of human endometrial epithelial cells (EECs) was collected for determination of secretion of cholesterol sulfate (CS). Total RNAs were extracted from human endometrial tissues and cultured cells for real-time reverse transcriptase-polymerase chain reaction (RT-PCR).
  
  
  METHODS
  The expression of SULT2B1b mRNA in human endometrial tissues and cultured cells.
  
  
  RESULTS
  In situ hybridization studies and real-time RT-PCR showed that the amount of SULT2B1b mRNA in human endometrial tissues was significantly higher during the midluteal phase than during other phases of the menstrual cycle. The secretion of CS from EECs was confirmed using [(35)S]-phosphoadenosine phosphosulfate. The expression of SULT2B1b mRNA was induced by cAMP or P in human endometrial stromal cells (ESCs), whereas it was induced by cAMP or relaxin in EECs. The induction of SULT2B1b mRNA by P or relaxin was abolished by the specific protein kinase A (PKA) inhibitor, Rp-adenosine 3',5' cyclic monophosphothioate (Rp-cAMPS).
  
  
  CONCLUSIONS
  The expression of SULT2B1b mRNA in ESCs is induced by P and that in EECs is induced by relaxin via the cAMP pathway.
authors.affiliation: Department of Obstetrics and Gynecology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. momoeda-tky@umin.ac.jp
authors.forename: Minako
authors.initials: M
authors.lastname: Koizumi
authors.forename: Mikio
authors.initials: M
authors.lastname: Momoeda
authors.forename: Hisahiko
authors.initials: H
authors.lastname: Hiroi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsutsumi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Osuga
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002788:Cholesterol Esters / Q000378:metabolism*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000601:surgery; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007044:Hysterectomy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008597:Menstrual Cycle; D011374:Progesterone / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D012065:Relaxin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects / Q000201:enzymology*; D015238:Sulfotransferases / Q000235:genetics / Q000378:metabolism*; D013873:Thionucleotides / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002788:Cholesterol Esters; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D013873:Thionucleotides; C016957:adenosine-3',5'-cyclic phosphorothioate; D011374:Progesterone; D012065:Relaxin; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D015238:Sulfotransferases; C433248:SULT2B1 protein, human; C007045:cholesteryl sulfate