Isolation of azaspiracid-2 from a marine sponge Echinoclathria sp. as a potent cytotoxin.
Azaspiracid-2 was isolated from a marine sponge Echinoclathria sp. collected off Amami-Oshima as the predominant cytotoxic constituent. A combination of HPLC using ODS, GS320, and Phenylhexyl stationary phases permitted the purification without using acid or inorganic additives in the mobile phase. Azaspiracid-2 exhibited potent cytotoxicity against P388 cells with an IC50 value of 0.72 ng/mL and caused S phase arrest on the cell cycle.
Ueoka
Reiko
R
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Ito
Akihiro
A
Izumikawa
Miho
M
Maeda
Satoko
S
Takagi
Motoki
M
Shin-ya
Kazuo
K
Yoshida
Minoru
M
van Soest
Rob W M
RW
Matsunaga
Shigeki
S
D000818:Animals; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy; D011161:Porifera / Q000378:metabolism*; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000095702:Polyether Toxins
D003603:Cytotoxins; D005663:Furans; D011714:Pyrans; C520447:azaspiracid-2; D000095702:Polyether Toxins