title: Regulation of persistent Na current by interactions between beta subunits of voltage-gated Na channels.
abstract: The beta subunits of voltage-gated Na channels (Scnxb) regulate the gating of pore-forming alpha subunits, as well as their trafficking and localization. In heterologous expression systems, beta1, beta2, and beta3 subunits influence inactivation and persistent current in different ways. To test how the beta4 protein regulates Na channel gating, we transfected beta4 into HEK (human embryonic kidney) cells stably expressing Na(V)1.1. Unlike a free peptide with a sequence from the beta4 cytoplasmic domain, the full-length beta4 protein did not block open channels. Instead, beta4 expression favored open states by shifting activation curves negative, decreasing the slope of the inactivation curve, and increasing the percentage of noninactivating current. Consequently, persistent current tripled in amplitude. Expression of beta1 or chimeric subunits including the beta1 extracellular domain, however, favored inactivation. Coexpressing Na(V)1.1 and beta4 with beta1 produced tiny persistent currents, indicating that beta1 overcomes the effects of beta4 in heterotrimeric channels. In contrast, beta1(C121W), which contains an extracellular epilepsy-associated mutation, did not counteract the destabilization of inactivation by beta4 and also required unusually large depolarizations for channel opening. In cultured hippocampal neurons transfected with beta4, persistent current was slightly but significantly increased. Moreover, in beta4-expressing neurons from Scn1b and Scn1b/Scn2b null mice, entry into inactivated states was slowed. These data suggest that beta1 and beta4 have antagonistic roles, the former favoring inactivation, and the latter favoring activation. Because increased Na channel availability may facilitate action potential firing, these results suggest a mechanism for seizure susceptibility of both mice and humans with disrupted beta1 subunits.
authors.affiliation: Interdepartmental Neuroscience Program, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: Teresa K
authors.initials: TK
authors.lastname: Aman
authors.forename: Tina M
authors.initials: TM
authors.lastname: Grieco-Calub
authors.forename: Chunling
authors.initials: C
authors.lastname: Chen
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rusconi
authors.forename: Emily A
authors.initials: EA
authors.lastname: Slat
authors.forename: Lori L
authors.initials: LL
authors.lastname: Isom
authors.forename: Indira M
authors.initials: IM
authors.lastname: Raman
mesh_terms: D000200:Action Potentials / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D015640:Ion Channel Gating / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D062550:NAV1.1 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D062547:Voltage-Gated Sodium Channel beta-1 Subunit; D062585:Voltage-Gated Sodium Channel beta-4 Subunit
chemical_list: D062550:NAV1.1 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D021122:Protein Subunits; C568242:SCN1A protein, human; C568249:Scn1a protein, mouse; C524974:Scn1b protein, mouse; C497241:Scn4b protein, mouse; D015222:Sodium Channels; D062547:Voltage-Gated Sodium Channel beta-1 Subunit; D062585:Voltage-Gated Sodium Channel beta-4 Subunit; D012964:Sodium