title: Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis).
abstract: The study was carried out to evaluate the potential impact of butylated hydroxytoluene (BHT) on the frozen-thawed semen quality of Nili-Ravi buffalo bulls. Ejaculated bull semen was extended in a Tris-citrate egg yolk extender containing various concentrations of BHT (0.5, 1.0, 2.0 and 3.0mM). Semen was frozen at -196 degrees C using 50 x 10(6) spermatozoa per 0.5 mL straws. Five straws from each treatment were thawed to assess the semen quality in terms of sperm motility, viability, plasma membrane integrity and acrosomal integrity. Post-thawed sperm motility was determined using a phase-contrast microscope. Viability, plasma membrane integrity and acrosomal integrity were evaluated by the supravital staining, hypo-osmotic swelling test and normal acrosomal reaction, respectively. The highest (P<0.05) motility, acrosomal integrity and hypo-osmotic swelling response of spermatozoa was achieved by addition of 1.0 and 2.0mM BHT to semen extender. However, highest (P<0.05) viability of spermatozoa was achieved by inclusion of 2.0mM BHT. The higher concentration of BHT (3.0mM) reduced the motility, acrosomal integrity, viability and hypo-osmotic swelling response of the spermatozoa compared to other concentration used. In conclusion, BHT when added in the semen extender can improve the semen quality of buffalo bulls.
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan. drijaz80@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Ijaz
authors.forename: A
authors.initials: A
authors.lastname: Hussain
authors.forename: M
authors.initials: M
authors.lastname: Aleem
authors.forename: M S
authors.initials: MS
authors.lastname: Yousaf
authors.forename: H
authors.initials: H
authors.lastname: Rehman
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D002020:Buffaloes / Q000502:physiology; D002084:Butylated Hydroxytoluene / Q000008:administration & dosage / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D012661:Semen / Q000187:drug effects*; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D002084:Butylated Hydroxytoluene