title: Use of rotorod as a method for the qualitative analysis of walking in rat.
abstract: High speed video analysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs.These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments [corrected].
authors.affiliation: Department of Psychology and Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge. whishaw@uleth.ca
authors.forename: Ian Q
authors.initials: IQ
authors.lastname: Whishaw
authors.forename: Katie
authors.initials: K
authors.lastname: Li
authors.forename: Paul A
authors.initials: PA
authors.lastname: Whishaw
authors.forename: Bogdan
authors.initials: B
authors.lastname: Gorny
authors.forename: Gerlinde A
authors.initials: GA
authors.lastname: Metz
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004298:Dopamine / Q000172:deficiency; D009048:Motor Skills; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000503:physiopathology*; D051381:Rats; D013647:Task Performance and Analysis; D016138:Walking
chemical_list: D016627:Oxidopamine; D004298:Dopamine