Derivatization of 11-alpha-hydroxyprogesterone using phosphoramidite chemistry.
The well-known phosphoramidite chemistry routinely used in solid-phase oligonucleotide synthesis is exploited here in the preparation of steroid conjugates in solution. The applicability of the method is tested by conjugating one nucleosidic and one non-nucleosidic phosphoramidite building block to 11-alpha-hydroxyprogesterone. The suitability of one of the conjugates synthesized (5) for a competitive binding assay is also demonstrated.
Ketola
Janne
J
Genetic Screening, PerkinElmer, Turku, Finland.
Hurskainen
Pertti
P
Hovinen
Jari
J
D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006908:Hydroxyprogesterones / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*
D006908:Hydroxyprogesterones; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C030228:11-hydroxyprogesterone