[Effects of all-trans retinioic acid and tazarotene on MMP-1 and TIMP-1 expression in cultured human fibroblasts after heat shock].
OBJECTIVE
To investigate the molecular mechanism of dermal damage in heat shock-induced skin aging by observing the expressions of metalloproteinase-1 (MMP-1) and tissue inhibitor of MMP-1 (TIMP-1) in retinoic acid-treated cultured human fibroblasts with heat shock.


METHODS
Cultured human fibroblasts were treated with tazarotene or all-trans-retinioic acid (at-RA) after heat shock for 30 min in 43 degrees celsius; water bath. Twenty-four hours later, MMP-1 and TIMP-1 contents in the supernatant of the cell culture medium were measured using enzyme-linked immunosorbent assay (ELISA).


RESULTS
Both tazarotene and at-RA dose-dependently reduced the expression of MMP-1 and increased the expression of TIMP-1 in cultured human fibroblasts exposed to heat shock, and tazarotene produced stronger effect than at-RA.


CONCLUSIONS
Retinoic acid can reduce the expression of MMP-1 and increase the expression of TIMP-1 in cultured human fibroblasts, suggesting its therapeutic potential for heat shock-induced skin aging.
Liu
Ping
P
Department of Dermatology, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. medlp@163.com
Tan
Sheng-shun
SS
Wang
Qiong-yu
QY
Wang
Yong-xian
YX
D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018869:Heat-Shock Response; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D009539:Nicotinic Acids / Q000494:pharmacology*; D015595:Skin Aging / Q000528:radiation effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D012176:Retinoids
D020781:Matrix Metalloproteinase 1; D009539:Nicotinic Acids; D019715:Tissue Inhibitor of Metalloproteinase-1; D014212:Tretinoin; C086827:tazarotene; D012176:Retinoids; C522360:MMP1 protein, human