title: Distribution of lectin-bindings in the testis of the lesser mouse deer, Tragulus javanicus.
abstract: The distribution of lectin bindings in the testis of the smallest ruminant, lesser mouse deer (Tragulus javanicus), was studied using 12 biotinylated lectins specific for d-galactose (peanut agglutinin PNA, Ricinus communis agglutinin RCA I), N-acetyl-d-galactosamine (Dolichos biflorus agglutinin DBA, Vicia villosa agglutinin VVA, Soybean agglutinin SBA), N-acetyl-D-glucosamine and sialic acid (wheat germ agglutinin WGA, s-WGA), D-mannose and d-glucose (Lens culinaris agglutinin LCA, Pisum sativum agglutinin PSA, Concanavalin A Con A), L-fucose (Ulex europaeus agglutinin UEA I), and oligosaccharide (Phaseolus vulgaris agglutinin PHA-E) sugar residues. In Golgi-, cap-, and acrosome-phase spermatids, lectin-bindings were found in the acrosome (PNA, RCA I, VVA, SBA, WGA and s-WGA), and in the cytoplasm (PNA, RCA I, VVA, SBA, WGA, LCA, PSA, Con A and PHA-E). s-WGA binding was confined to the spermatid acrosome, but other lectins were also observed in spermatocytes. In spermatogonia, VVA, WGA, Con A, and PHA-E bindings were observed. Sertoli cells were intensely stained with DBA and Con A, and weakly with PHA-E. In interstitial Leydig cells, RCA I, DBA, VVA, Con A, PSA, LCA, WGA and PHA-E were positive. UEA I was negative in all cell types including spermatogenic cells. Unusual distribution of lectin-bindings noted in the testis of lesser mouse deer included the limited distribution of s-WGA only in the spermatid acrosome, the distribution of DBA in Sertoli cells, Leydig cells and lamina propria, and the absence of UEA I in all type cells. The present results were discussed in comparison with those of other animals and their possible functional implications.
authors.affiliation: Department of Anatomy, Physiology and Pharmacology, Bogor Agricultural University, Bogor 16680, Indonesia. ysrihadi@ipb.ac.id
authors.forename: S
authors.initials: S
authors.lastname: Agungpriyono
authors.forename: M
authors.initials: M
authors.lastname: Kurohmaru
authors.forename: J
authors.initials: J
authors.lastname: Kimura
authors.forename: A H
authors.initials: AH
authors.lastname: Wahid
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: N
authors.initials: N
authors.lastname: Kitamura
authors.forename: J
authors.initials: J
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Fukuta
authors.forename: A B
authors.initials: AB
authors.lastname: Zuki
mesh_terms: D000818:Animals; D001188:Artiodactyla / Q000033:anatomy & histology* / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D037102:Lectins / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D013737:Testis / Q000378:metabolism*
chemical_list: D037102:Lectins