Molecular and functional characterization of the plastid-localized Phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana.
The Arabidopsis thaliana gene At1g74030 codes for a putative plastid phosphoenolpyruvate (PEP) enolase (ENO1). The recombinant ENO1 protein exhibited enolase activity and its kinetic properties were determined. ENO1 is localized to plastids and expressed in most heterotrophic tissues including trichomes and non-root-hair cells, but not in the mesophyll of leaves. Two T-DNA insertion eno1 mutants exhibited distorted trichomes and reduced numbers of root hairs as the only visible phenotype. The essential role of ENO1 in PEP provision for anabolic processes within plastids, such as the shikimate pathway, is discussed with respect to plastid transporters, such as the PEP/phosphate translocator.
Prabhakar
Veena
V
Universität zu Köln, Botanisches Institut, Köln (Cologne), Germany.
Löttgert
Tanja
T
Gigolashvili
Tamara
T
Bell
Kirsten
K
Flügge
Ulf-Ingo
UI
Häusler
Rainer E
RE
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004789:Enzyme Activation; D007700:Kinetics; D009928:Organ Specificity / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D018087:Plastids / Q000378:metabolism*; D014018:Tissue Distribution
D010751:Phosphopyruvate Hydratase