Fast two-dimensional NMR spectroscopy of high molecular weight protein assemblies.
An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl (1)H-(13)C correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.
Amero
Carlos
C
Institut de Biologie Structurale Jean-Pierre Ebel CNRS-CEA-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.
Schanda
Paul
P
Durá
M Asunción
MA
Ayala
Isabel
I
Marion
Dominique
D
Franzetti
Bruno
B
Brutscher
Bernhard
B
Boisbouvier
Jérôme
J
D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D011506:Proteins