Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies.
The human antibody response to polyomavirus capsid proteins is not well characterized. Recombinant BK virus (BKV), JC virus (JCV) and simian virus 40 (SV40) virus-like particles (VLP) were produced in a baculovirus system, and mouse monoclonal antibodies (mAbs) to these proteins were generated using standard methods. Nine of 12 BKV mAbs showed neutralizing activity. The non-neutralizing antibodies also bound BKV pseudocapsids in an ELISA binding assay. Most antibodies recognized conformational species-specific epitopes, but several exceptions were found: (i) BKV mAb BK-F11 cross-reacted with a linear buried epitope common to both JCV and SV40 pseudocapsids, (ii) two of six JCV antibodies (JC-6.7 and JC-7.9) and two of 13 SV40 antibodies (VP1-H2 and VP1-I2) recognized linear buried epitopes common to all three viruses and (iii) SV40 antibody VP1-E5 recognized a linear surface epitope on JCV pseudocapsids.
Randhawa
Parmjeet
P
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Viscidi
Raphael
R
Department of Pediatrics, Johns Hopkins University, Baltimore, MD, USA.
Carter
Joseph J
JJ
Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA.
Galloway
Denise A
DA
Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA.
Culp
Tim D
TD
Department of Pathology, Jake Gittlen Cancer Research Foundation, Pennsylvania State University, Hershey, USA.
Huang
Cathy
C
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Ramaswami
Bala
B
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Christensen
Neil D
ND
Department of Pathology, Jake Gittlen Cancer Research Foundation, Pennsylvania State University, Hershey, USA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001739:BK Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016367:Baculoviridae / Q000378:metabolism; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology*; D003429:Cross Reactions; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes