title: Effect of anti-GM2 antibodies on rat sciatic nerve: electrophysiological and morphological study.
abstract: We found that a monoclonal human IgM anti-GM2 was fixed in rat sciatic axons and Schwann cells and was able to activate human complement. The passive transfer of IgM and complement in sciatic nerves can induce an acute alteration in nerve conduction. When the transfer of IgM plus complement was repeated for 10 days, the compound action motor potential amplitude was very low and the morphological study showed axons and myelin damage. Without human complement, IgM can only slightly disorganize the myelin by separating some layers, probably by interfering with the functional role of gangliosides in the myelin package.
authors.affiliation: Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain. nicolauantoni.ortiz@urv.net
authors.forename: Nicolau
authors.initials: N
authors.lastname: Ortiz
authors.forename: M Mar
authors.initials: MM
authors.lastname: Sabaté
authors.forename: Neus
authors.initials: N
authors.lastname: Garcia
authors.forename: Manel M
authors.initials: MM
authors.lastname: Santafe
authors.forename: M Angel
authors.initials: MA
authors.lastname: Lanuza
authors.forename: Marta
authors.initials: M
authors.lastname: Tomàs
authors.forename: Josep
authors.initials: J
authors.lastname: Tomàs
mesh_terms: D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology; D001323:Autoantibodies / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D002908:Chronic Disease; D003711:Demyelinating Diseases / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D005678:G(M2) Ganglioside / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology; D008297:Male; D015213:Neuroimmunomodulation / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure*
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007075:Immunoglobulin M; D005678:G(M2) Ganglioside