title: Detection of typical and atypical bovine spongiform encephalopathy and scrapie prion strains by prion protein motif-grafted antibodies.
abstract: To evaluate further the reactivity of prion-specific monoclonal antibodies containing the 89-112 or 136-158 prion protein (PrP) polypeptides, immunoprecipitations were performed on brain extracts from Italian bovines, sheep and goats with transmissible spongiform encephalopathies. No binding of IgG 89-112 or IgG 136-158 to PrP in normal brain extracts was detected. Conversely, both reagents immunoprecipitated PrP from bovine and bovine amyloidotic spongiform encephalopathies, and from typical and atypical scrapie brain extracts. The immunoprecipitated PrP bands mirrored the Western blot (WB) profile of the different prion strains, indicating universal affinity of two independent PrP regions for disease-associated PrP conformers regardless of species source and strain properties. Immunoprecipitation with motif-grafted antibodies increased the sensitivity of conventional detection methods based on centrifugation followed by WB, which was confirmed by assay of diluted samples using both methods. These reagents or derivative molecules may thus find broad applications in prion detection and research.
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Martucci
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Acutis
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Mazza
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Nodari
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Colussi
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Corona
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Contrada S. Martino 6, 63023 Fermo, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Barocci
authors.affiliation: Clinica Medica, Dipartimento di Scienze Mediche e Chirurgiche, Università Politecnica delle Marche, via Tronto 10, 60126 Ancona, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: Gabrielli
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Caramelli
authors.affiliation: CEA, Italian Reference Laboratory for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Casalone
authors.affiliation: Clinica Medica, Dipartimento di Scienze Mediche e Chirurgiche, Università Politecnica delle Marche, via Tronto 10, 60126 Ancona, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Moroncini
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis / Q000378:metabolism; D015511:Goat Diseases / Q000175:diagnosis / Q000378:metabolism; D006041:Goats; D047468:Immunoprecipitation; D018620:PrPSc Proteins / Q000276:immunology; D017096:Prion Diseases / Q000175:diagnosis / Q000378:metabolism; D011328:Prions / Q000737:chemistry / Q000276:immunology*; D012608:Scrapie / Q000175:diagnosis / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D018620:PrPSc Proteins; D011328:Prions