[Effect of AT-1 alpha receptor gene silencing on nuclear factor-kappaB activity in hepatic Kupffer cells].
OBJECTIVE
To investigate the effect of angiotensin II type-1 (AT-1) alpha receptor gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic Kupffer cells.


METHODS
The expression of AT-1 alpha receptors in primary isolated cultured hepatic Kupffer cells was detected by immunohistochemistry. pSilencer/AT-1 alpha receptor siRNA plasmids were transfected into Kupffer cells, which were subsequently exposed to 10(-6) mol/L angiotensin II (Ang II) for 60 min. The changes in the DNA binding activity of NF-kappaB in the cells was assessed using electrophoretic gel mobility shift assay (EMSA).


RESULTS
AT-1 alpha receptor expression was detected in Kupffer cells. NF-kappaB DNA binding activity was markedly increased in Kupffer cells after Ang II stimulation, and obviously inhibited by transfectiom with pSilencer/AT-1 alpha receptor siRNA plasmid.


CONCLUSIONS
Ang II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor.
Li
Xu
X
Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. mylx99@163.com
Meng
Ying
Y
Zhou
Gao-su
GS
Zhang
Zhen-shu
ZS
D002478:Cells, Cultured; D006801:Humans; D007728:Kupffer Cells / Q000166:cytology*; D016328:NF-kappa B / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics* / Q000378:metabolism
D016328:NF-kappa B; D034741:RNA, Small Interfering; D044140:Receptor, Angiotensin, Type 1