Positive regulation of minichromosome maintenance gene expression, DNA replication, and cell transformation by a plant retinoblastoma gene.
Retinoblastoma-related (RBR) genes inhibit the cell cycle primarily by repressing adenovirus E2 promoter binding factor (E2F) transcription factors, which drive the expression of numerous genes required for DNA synthesis and cell cycle progression. The RBR-E2F pathway is conserved in plants, but cereals such as maize are characterized by having a complex RBR gene family with at least 2 functionally distinct members, RBR1 and RBR3. Although RBR1 has a clear cell cycle inhibitory function, it is not known whether RBR3 has a positive or negative role. By uncoupling RBR3 from the negative regulation of RBR1 in cultured maize embryos through a combination of approaches, we demonstrate that RBR3 has a positive and critical role in the expression of E2F targets required for the initiation of DNA synthesis, DNA replication, and the efficiency with which transformed plants can be obtained. Titration of endogenous RBR3 activity through expression of a dominant-negative allele with a compromised pocket domain suggests that these RBR3 functions require an activity distinct from its pocket domain. Our results indicate a cell cycle pathway in maize, in which 2 RBR genes have specific and opposing functions. Thus, the paradigm that RBR genes are negative cell cycle regulators cannot be considered universal.
Sabelli
Paolo A
PA
Department of Plant Sciences, University of Arizona, 303 Forbes Hall, Tucson, AZ 85721, USA. psabelli@ag.arizona.edu
Hoerster
George
G
Lizarraga
Lucina E
LE
Brown
Sara W
SW
Gordon-Kamm
William J
WJ
Larkins
Brian A
BA
D032461:Chromosomes, Plant / Q000235:genetics*; D004261:DNA Replication; D015536:Down-Regulation / Q000235:genetics; D016195:G2 Phase; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D016161:Genes, Retinoblastoma; D008957:Models, Genetic; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D003313:Zea mays / Q000166:cytology* / Q000235:genetics*
D010940:Plant Proteins; D012333:RNA, Messenger