title: Production of chromopyrrolic acid by coexpression of inkOD in a heterologous host Streptomyces albus.
abstract: Two ink genes, inkO and inkD, responsible for the earliest steps of K252a biosynthetic pathway, from Nonomurea longicantena JCM 11136 were heterologously coexpressed in Streptomycesalbus J1074. The resultant strain accumulated compound that was purified by HPLC and studied by NMR. Coexpression of inkOD yielded chromopyrrolic acid, the key intermediate in an indolocarbazole biosynthesis.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Choong-Sik
authors.initials: CS
authors.lastname: Chae
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Park
authors.forename: Soon-Chun
authors.initials: SC
authors.lastname: Chung
authors.forename: Tae-Im
authors.initials: TI
authors.lastname: Kim
authors.forename: So-Hyoung
authors.initials: SH
authors.lastname: Lee
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Yoon
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Oh
mesh_terms: D002227:Carbazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D015195:Drug Design; D026121:Indole Alkaloids / Q000138:chemical synthesis / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D005810:Multigene Family; D011758:Pyrroles / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry
chemical_list: D002227:Carbazoles; D026121:Indole Alkaloids; D011758:Pyrroles; C541708:chromopyrrolic acid; D014364:Tryptophan; C049985:staurosporine aglycone