Inorganic phosphate regulates Glvr-1 and -2 expression: role of calcium and ERK1/2.
Sodium-dependent phosphate cotransporters are key regulators of phosphate homeostasis and play a major role in mineralized tissues remodelling. However, factors influencing their expression remain under consideration. In our study, modulation of type III sodium-dependent phosphate cotransporters expression by inorganic phosphate (Pi) was investigated in the murine odontoblast-like cell line MO6-G3. Experiments were designed to determine the effects of phosphate release on dental cells during tooth decay. By real-time RT-PCR we demonstrated that Glvr-1 and -2 expressions are up-regulated by Pi. The increase in Glvr-1 and -2 expressions was correlated with ERK1/2 phosphorylation and calcium/phosphate crystals formation in cultured wells. Using calcium-free culture conditions or the specific inhibitor of ERK phosphorylation (UO126), we demonstrated that Pi effects on Glvr-1 and -2 up-regulation require the presence of calcium and involve ERK signalling pathways. This study contributes to give new insights in the control of Pi transport during carious diseases.
Wittrant
Y
Y
INSERM Unité 791, Université de Nantes, Faculté de Chirurgie Dentaire, Nantes, France. yohann.wittrant@univ-nantes.fr
Bourgine
A
A
Khoshniat
S
S
Alliot-Licht
B
B
Masson
M
M
Gatius
M
M
Rouillon
T
T
Weiss
P
P
Beck
L
L
Guicheux
J
J
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003731:Dental Caries / Q000378:metabolism*; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology*; D010766:Phosphorylation; D011991:Receptors, Virus / Q000096:biosynthesis*; D050611:Sodium-Phosphate Cotransporter Proteins, Type III / Q000096:biosynthesis*
D010710:Phosphates; D011991:Receptors, Virus; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; C067981:leukemia virus receptor, gibbon ape; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D002118:Calcium