[Analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector].
OBJECTIVE
To establish a new method for the analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector.


METHODS
An initial procedure of precipitation was performed by adding hydrochloric solution with sodium chloride and a mixture of chloroform and ethanol. Then the analyte contained in supernatant was reduced by a reduction system of sodium borohydride and nickel chloride and extracted by acetic ether. Ethyl paraquat (EPQ) was used as internal standard. GC/TSD was used to identify and quantify the analyte.


RESULTS
The limits of detection (S/N=3) in blood and urine were 0.002 and 0.004 microg/mL, respectively. The linear ranges were 0.050-30.0 microg/mL. Correlation coefficients in blood and urine were 0.999 and 0.998, respectively. The recoveries exceeded 80% both in blood and urine.


CONCLUSIONS
This method is applicable for quantification of paraquat in biological fluids.
Huang
Lu-Yao
LY
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liao
Lin-chuan
LC
Chen
Li-Li
LL
Su
Gui
G
Li
Wen-Jia
WJ
He
Rong
R
Yan
You-Yi
YY
Yang
Lin
L
D001894:Borohydrides / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods*; D053593:Forensic Toxicology; D006540:Herbicides / Q000097:blood / Q000652:urine; D006801:Humans; D009532:Nickel / Q000737:chemistry*; D010084:Oxidation-Reduction; D010269:Paraquat / Q000097:blood* / Q000652:urine*; D012680:Sensitivity and Specificity
D001894:Borohydrides; D006540:Herbicides; C022838:nickel chloride; D009532:Nickel; C025364:sodium borohydride; D010269:Paraquat