Accelerated wound healing in healing-impaired db/db mice by autologous adipose tissue-derived stromal cells combined with atelocollagen matrix.
Adipose tissue-derived stromal cells (ATSCs) have recently gained widespread attention as a potential alternate source to bone marrow-derived mesenchymal stem cells with a proliferative capacity and a similar ability to undergo multilineage differentiation. In this study, we evaluated the effectiveness of freshly isolated autologous ATSCs-containing atelocollagen matrix with silicon membrane (ACMS) on wound healing of diabetic (db/db) mice. Cultured ATSCs from (db/db) mice secreted significant amounts of growth factors and cytokines, which are suitable for wound repair. Two full thickness round skin defects were made on the backs of healing-impaired db/db mice. Freshly isolated autologous ATSCs-containing ACMS or ACMS alone were applied to the wounds. Twelve mice were treated and then killed at 1 or 2 weeks (n = 6 each). Histologic sections of the wounds were prepared at each time period after treatment. Histologic examination demonstrated significantly advanced granulation tissue formation, capillary formation, and epithelialization in diabetic healing-impaired wounds treated with autologous ATSCs-containing ACMS, compared with mice treated with ACMS alone. These results suggested that transplantation of autologous ATSCs-containing ACMS significantly accelerated wound healing in diabetic healing-impaired db/db mice.
Nambu
Masaki
M
Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
Kishimoto
Satoko
S
Nakamura
Shingo
S
Mizuno
Hiroshi
H
Yanagibayashi
Satoshi
S
Yamamoto
Naoto
N
Azuma
Ryuichi
R
Nakamura
Shin-ichiro
S
Kiyosawa
Tomoharu
T
Ishihara
Masayuki
M
Kanatani
Yasuhiro
Y
D000273:Adipose Tissue; D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D012825:Silicon; D017768:Skin, Artificial; D017154:Stromal Cells / Q000637:transplantation*; D014182:Transplantation, Autologous; D014945:Wound Healing
D001672:Biocompatible Materials; C059821:atelocollagen; D003094:Collagen; D012825:Silicon