title: Temperature dependency for survival of Mycobacterium leprae in macrophages.
abstract: Hansen's disease is caused by an infection with an intracellular pathogen, Mycobacterium leprae, which mainly inhabits macrophages and Schwann cells. However, little is known about the survival or growth mechanisms of the bacilli in mouse and human macrophages. In the present study, by using radiorespirometry analysis for the evaluation of the viability of M. leprae, we observed that in vitro incubation of M. leprae-infected macrophages at 35 degrees C was more growth permissive than at 37 degrees C, and supplementation with the immunosuppressive cytokine IL-10 supported the survival of the bacilli in the macrophages for 3 weeks, whereas viability of the bacilli was gradually lost if cultured without IL-10. In human macrophages, M. leprae retained its viability when cultured at 35 degrees C for at least 4 weeks without IL-10. However, the viability of M. leprae was almost lost within 2 weeks if cultured at 37 degrees C. These data suggest that temperature is a crucial factor for the survival of M. leprae in host cells.
authors.affiliation: Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1, Aoba-cho, Higashimurayama-shi, Tokyo 189-0002, Japan. fukutomi@nih.go.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Fukutomi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Maeda
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Masahiko
authors.initials: M
authors.lastname: Makino
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008819:Mice, Nude; D050296:Microbial Viability / Q000187:drug effects*; D009166:Mycobacterium leprae / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D016207:Cytokines; D016212:Transforming Growth Factor beta; D016753:Interleukin-10