[0507JS60 virus isolated in Xinjiang was identified as Liaoning virus].
0507JS60 virus was isolated from a pool of Culex sp. collected in Kashi, Xinjiang, which could be propagated stably on C6/36 cells and caused cytopathic effects continuously. Viral particles had no envelope and appeared round with diameter of about 55nm (n = 10). Capsomeres on the surface of capsid were clearly visible. Electrophoresis of viral genome showed a profile of 12 double stranded RNA (dsRNA) segments. Sequencing of the twelfth segment revealed the length of 760bp (GenBank ID: FJ157354). A single open reading frame (ORF) was found and encoded a protein of 174 amino acids with a molecular mass of 18.9kD. The nucleotide sequence had similarity over 89% with that of LNV, but the deduced amino acid sequence had similarity over 91% with that of LNV. A phylogenetic tree was constructed to compare the corresponding genetic sequences in Seadornavirus. The tree demonstrated that 0507JS60 virus lied in the same branch with LNV and more closely related to LNV-NE9712. 0507JS60 virus was identified as LNV, which was firstly isolated outside the Northeast of China.
Lv
Xin-Jun
XJ
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Lv
Zhi
Z
Sun
Xiao-Hong
XH
Fu
Shi-Hong
SH
Wang
Huan-Qin
HQ
Tong
Su-Xiang
SX
Zhang
Song
S
Attoui
H
H
Liang
Guo-Dong
GD
D000818:Animals; D002460:Cell Line; D002681:China; D003465:Culex / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure