[Effect of intense pulsed light on transforming growth factor-beta1 mRNA expression in rat skin].
OBJECTIVE
To observe the effect of intense pulsed light (IPL) on transforming growth factor-beta1 mRNA (TGF-beta1 mRNA) expression in rat skin and explore the molecular mechanisms of photorejuvenation.


METHODS
Fifteen SD rats were exposed to IPL in 3 dermal regions with triple pulses (duration of 4, 5, and 6 ms) at the energy density of 34 J/cm2 and pulse delay of 20 or 25 ms. On days 1, 3, 5, 7, 15, and 30 after the treatment, skin specimens from the treated and non-treated areas were obtained to detect TGF-beta1 mRNA expression with in situ hybridization.


RESULTS
In the UPL-exposed skin areas, TGF-beta1 mRNA expression was detected in the epidermal keratinocytes and dermal cells 1 day after the exposure, reaching the highest expression level on day 7 followed by gradual decrement since day 15, and till day 30, only weak expression was found in the dermal cells. In the non-exposed regions, the cells remained negative for TGF-beta1 mRNA.


CONCLUSIONS
IPL can enhance TGF-beta1 mRNA expression in the skin, suggesting that TGF-beta1 plays an important role in dermal remodeling in photorejuvenation.
Wang
Ming-li
ML
Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Liu
Da-lie
DL
Yuan
Qiang
Q
Du
Ben-jun
BJ
D000818:Animals; D005260:Female; D008297:Male; D010789:Phototherapy / Q000009:adverse effects / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012060:Rejuvenation; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*
D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1