title: Gene expression analysis by oligonucleotide microarray in oral leukoplakia.
abstract: BACKGROUND
  Oral leukoplakias (LP) are the most frequent types of oral pre-cancerous lesions, but there is no accurate assessment of this malignant transformation or even genetic diagnosis of the oral epithelial dysplasia. We need to identify the new genetic diagnosis system of the epithelial dysplasia.
  
  
  METHODS
  Oligonucleotide microarray was used to analyze expression patterns of 29,952 genes in 10 LP patients. We compared the different gene expressions between mild dysplasia cases and severe dysplasia cases.
  
  
  RESULTS
  Ninety-six genes expressed differentially were selected as candidates for up-regulated in severe dysplasia. Subsequently, we further selected 16 genes with highest differentially expression. By hierarchical clustering analysis, the 10 cases were divided mild dysplasia from severe dysplasia.
  
  
  CONCLUSIONS
  The 16 genes are suggested as biomarker gene sets of efficacy and quickly recognized in the development of oral epithelial dysplasia.
authors.affiliation: Oral and Maxillofacial Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. kuriosur@tmd.ac.jp
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kuribayashi
authors.forename: Kei-ichi
authors.initials: K
authors.lastname: Morita
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tomioka
authors.forename: Masaru
authors.initials: M
authors.lastname: Uekusa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Ken
authors.initials: K
authors.lastname: Omura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D016000:Cluster Analysis; D004848:Epithelium / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation