The effect of in vitro gamma-irradiation on mitogenic responsiveness of murine lymphocytes.
The objective of this study was to analyze the proliferative response of BALB/c mice lymphocytes after in vitro irradiation (0.05 to 6 Gy). The capability of irradiated lymphocytes for proliferating without any stimulation and after activation with specific T and B cell mitogens has been evaluated. The results show that ionizing radiation significantly inhibits spontaneous cellular proliferation and that induced by mitogens and that variations in the degree of inhibition are found depending on the inducing proliferation mitogens and the dosage applied. The conclusion drawn is that different lymphocyte populations have different radiosensitivities, being B cells more sensitive to ionizing irradiation than T cells. Besides, the effects of gamma-irradiation vary according to the different subpopulations of T cells or, alternatively, to different T-dependent activation mechanisms.
Hernández-Godoy
J
J
Centro de Investigación, Unidad de Inmunología Experimental, Hospital La Fe, Avda. Campanar s/n, 46009 Valencia, Spain.
Planelles
D
D
Balsalobre
B
B
González-Molina
A
A
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000008:administration & dosage; D004306:Dose-Response Relationship, Immunologic; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000008:administration & dosage* / Q000276:immunology; D010835:Phytohemagglutinins / Q000008:administration & dosage; D011043:Pokeweed Mitogens / Q000008:administration & dosage; D011836:Radiation Tolerance / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects
D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A