title: [A quantitative method using one marker for simultaneous assay of ginsenosides in Panax ginseng and P. notoginseng].
abstract: Current quality control patterns are limited to industrial application, for most of the natural chemical reference substances are expensive and unavailable. Herein, a method, quantitative analysis of multi-components with single marker (QAMS), was established and validated to simultaneously determine nine ginsenosides (ginsenoside Rg1, Re, Rf, Rh1, Rb1, Rc, Rb2, Rb3, Rd) in P. ginseng and four ginsenosides (ginsenoside Rg1, Rh1, Rb1, Rd) in P. notoginseng. Using ginsenoside Rb1 as the contrast, the relative correction factors (RCF) of the other eight ginsenosides were determined by HPLC-DAD. Within the linear ranges, the values of RCF of ginsenoside Rb1 to ginsenoside Rg1, Re, Rf, Rh1, Rc, Rb2, Rb3 and Rd were 1.400, 1.215, 1.517, 1.801, 0.944, 1.012, 1.143, and 1.135, respectively. The RCF had a good reproducibility in various instruments, chromatographic columns (RSD = 0.30% - 3.9%). According to their RCF, we simultaneously determined nine ginsenosides in P. ginseng only using one marker. In addition, the RCF of ginsenosides were used to simultaneously quantitative analysis of four ginsenosides in P. notoginseng. The results of QAMS method were validated by comparing with that of external standard method, and no obvious significant difference was found.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Zhu
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Kuang
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Qi-pin
authors.initials: QP
authors.lastname: Gao
authors.forename: Ni
authors.initials: N
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D036145:Ginsenosides; C044462:ginsenoside Rc; C048026:ginsenoside Rb2; C425564:ginsenoside Rh1; C044463:ginsenoside Rb3; C055328:ginsenoside Rf; C049863:ginsenoside Rd