title: Evaluation of a rapid fluorescent staining method for detection of mycobacteria in clinical specimens.
abstract: Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time ( approximately 2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories.
authors.affiliation: The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA.
authors.forename: Cindy
authors.initials: C
authors.lastname: Hendry
authors.forename: Kim
authors.initials: K
authors.lastname: Dionne
authors.forename: Annie
authors.initials: A
authors.lastname: Hedgepeth
authors.forename: Karen
authors.initials: K
authors.lastname: Carroll
authors.forename: Nicole
authors.initials: N
authors.lastname: Parrish
mesh_terms: D001576:Benzophenoneidum / Q000494:pharmacology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D005456:Fluorescent Dyes; D001576:Benzophenoneidum