title: Monoaminergic changes in locus coeruleus and dorsal raphe nucleus following noradrenaline depletion.
abstract: The goal of our study was to assess the monoaminergic changes in locus coeruleus (LC) and dorsal raphe nucleus (DRN) following noradrenaline (NA) depletion. Seven days after a single N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4) intraperitoneal administration in mice, we observed a decrease of NA in both the LC and DRN, as well as in prefrontal cortex (PFC) and hippocampus (HIPP). Moreover, an increase of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) was detected at LC level, while no change was found in DRN. DSP-4 also caused a significant decrease of dopamine (DA) tissue content in HIPP and DRN, without affecting the LC and the PFC. A decrease of DA metabolite, homovanillic acid (HVA), was found in the DRN of NA-depleted mice. These results highlight that the neurotoxic action of DSP-4 is not restricted to LC terminal projections but also involves NA depletion at the cell body level, where it is paralleled by adaptive changes in both serotonergic and dopaminergic systems.
authors.affiliation: Department of Biomedical Sciences, University of Foggia, Viale Luigi Pinto, 1, 71100 Foggia, Italy. tommaso.cassano@unifg.it
authors.forename: Tommaso
authors.initials: T
authors.lastname: Cassano
authors.forename: Silvana
authors.initials: S
authors.lastname: Gaetani
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Morgese
authors.forename: Teresa
authors.initials: T
authors.lastname: Macheda
authors.forename: Leonardo
authors.initials: L
authors.lastname: Laconca
authors.forename: Pasqua
authors.initials: P
authors.lastname: Dipasquale
authors.forename: Juan
authors.initials: J
authors.lastname: Taltavull
authors.forename: Toni S
authors.initials: TS
authors.lastname: Shippenberg
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cuomo
authors.forename: Gabriella
authors.initials: G
authors.lastname: Gobbi
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D015306:Biogenic Monoamines / Q000378:metabolism*; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008125:Locus Coeruleus / Q000378:metabolism*; D008297:Male; D051379:Mice; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009638:Norepinephrine / Q000502:physiology*; D011903:Raphe Nuclei / Q000378:metabolism*
chemical_list: D001596:Benzylamines; D015306:Biogenic Monoamines; D014179:Neurotransmitter Uptake Inhibitors; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; C012102:DSP 4; D009638:Norepinephrine; D006719:Homovanillic Acid