[Targeted delivery of bone marrow mesenchymal stem cells by ultrasound-mediated microbubble destruction].
OBJECTIVE
To investigate the feasibility of bone marrow mesenchymal stem cell (MSC) transplantation with ultrasound-targeted microbubble destruction.


METHODS
Twenty-one Wistar rats were divided into MSCs-iv group (MSCs-iv), ultrasound+MSCs-iv group (US+MSCs-iv), ultrasound+microbubble+MSCs-iv group (US+MB+MSCs-iv) with intravenous MSC transfer, ultrasound and microbubble treatment as indicated. The skeletal muscles were obtained from the rats for microscopic examination with HE staining. The hindlimb gracilis and semimembranosus muscles were sampled 7 days after MSC transplantation, and the transplanted MSCs were detected by immunohistochemistry. The vital organs were collected from rats in US+MB+MSCs-iv group for immunohistochemistry.


RESULTS
In US+MB+MSCs-iv group, HE staining demonstrated the presence of red blood cell leakage into the tissue space in the gracilis and semimembranosus muscles, and immunohistochemistry identified large numbers of transplanted MSCs in the the gracilis and semimembranosus muscles and the spleen, whereas no labeled cells were detected in the skeletal muscles in other groups.


CONCLUSIONS
Ultrasound-targeted microbubble destruction provides a useful means for enhancing the efficiency of stem cell transplantation.
Bai
Yuan-yuan
YY
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. baiyy1983@163.com
Xiu
Jian-cheng
JC
Zha
Dao-gang
DG
Wang
Yue-gang
YG
Liu
Yi-li
YL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement / Q000528:radiation effects; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D045423:Microbubbles; D018482:Muscle, Skeletal / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D014465:Ultrasonics