Fluorescent color readout of DNA hybridization with thiazole orange as an artificial DNA base.
A fluorescent chameleon: A single thiazole orange (TO) dye, when used as an artificial DNA base shows the typical green emission, whereas the interstrand TO dimer exhibits an orange excimer-type emission inside duplex DNA (see picture).
Berndl
Sina
S
Institute for Organic Chemistry, University of Regensburg, 93040 Regensburg, Germany.
Wagenknecht
Hans-Achim
HA
D001483:Base Sequence; D052160:Benzothiazoles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011804:Quinolines; C051000:thiazole orange; D004247:DNA