Effect of potassium diformate on growth performance and gut microbiota in broiler chickens challenged with necrotic enteritis.
1. The effect of potassium diformate (KDF) on mortality, growth performance, microbial populations, pH and short chain fatty acid concentrations in the intestinal tract of broiler chickens challenged with necrotic enteritis was investigated. 2. An experiment with 1050 Cobb male broiler chickens was conducted from 1 to 35 d of age. There were 7 treatment groups: (1) unchallenged negative control, (2) unchallenged KDF (4.50 g/kg feed), (3) challenged negative control, (4) challenged positive control (100 ppm monensin and 45 ppm Zn-bacitracin), and (5) to (7) challenged KDF (inclusion rate 2.25, 4.50 and 6.75 g/kg feed, respectively). 3. The necrotic enteritis challenge caused significant growth depression except in the challenged positive control group. Inclusion of KDF in feed had no significant effect on weight gain or feed conversion ratio, neither under challenged nor unchallenged conditions. Necrotic-enteritis-related mortalities were reduced in response to the positive control feed and KDF at 2.25 and 4.50 g/kg rates, but with no effect at the 6.75 g/kg rate. 4. There was no effect of KDF on Clostridium perfringens numbers in jejunum during the necrotic enteritis challenge (d 15). Post challenge (d 35), 4.5 g/kg KDF reduced the number of C. perfringens and Enterobacteria in jejunum compared with the negative control group. 5. Intestinal pH was not affected by adding KDF to the feed. The challenge reduced jejunum pH compared with the unchallenged treatment groups and challenged positive control group. 6. Chickens fed the KDF diets had detectable concentrations of formic acid in the jejunum. There was a tendency towards higher concentrations of acetic acid and lactic acid in the small intestine of unchallenged birds, whereas challenged birds had higher concentrations of butyric acid in the caeca. 7. It is concluded that KDF holds promise as an agent to control necrotic enteritis in broiler chickens and it may be possible to enhance the efficacy of KDF by manipulating dietary properties that may influence the dissociation kinetics of KDF in the gut.
Mikkelsen
L L
LL
School of Rural Science and Agriculture, University of New England, Armidale, NSW, Australia. lmikkels@une.edu.au
Vidanarachchi
J K
JK
Olnood
C G
CG
Bao
Y M
YM
Selle
P H
PH
Choct
M
M
D000818:Animals; D002645:Chickens; D003015:Clostridium Infections / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D003016:Clostridium perfringens / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000662:veterinary; D004751:Enteritis / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D005561:Formates / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007410:Intestinal Diseases / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D008297:Male; D009336:Necrosis; D017680:Potassium Compounds / Q000494:pharmacology*; D011201:Poultry Diseases / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*
D005561:Formates; D017680:Potassium Compounds