title: Formation of cyclodextrins with cyclodextrin glucosyltransferase stimulated with polarized light.
abstract: After illumination with white, linearly polarized light (WLPL), cyclodextrin glycosyltransferase produced mixture of alpha-, beta-, and gamma-cyclodextrins (CD) with higher overall yield than did that enzyme when nonilluminated. The illumination also influenced the ratio of those CD and that effect depended on concentration of enzyme and illumination time. At a high enzyme concentration (0.64 U/cm(3)), regardless the illumination time, formation of beta-CD predominated. The highest yield of beta-CD was afforded after 1 h illumination and 2 h illumination led to a significant increase in the yield of gamma-CD. Three-month storage of enzyme illuminated with WLPL did not reduce its enhanced activity.
authors.affiliation: Dept. of Chemistry, University of Agriculture, Cracow 30-149, Poland. rrfiedor@cyf-kr.edu.pl
authors.forename: Maciej
authors.initials: M
authors.lastname: Fiedorowicz
authors.forename: Gohar
authors.initials: G
authors.lastname: Khachatryan
authors.forename: Anna
authors.initials: A
authors.lastname: Konieczna-Molenda
authors.forename: Piotr
authors.initials: P
authors.lastname: Tomasik
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism* / Q000528:radiation effects*; D007700:Kinetics; D008027:Light; D047391:alpha-Cyclodextrins / Q000737:chemistry / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism; D047408:gamma-Cyclodextrins / Q000737:chemistry / Q000378:metabolism
chemical_list: D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase