Plasma pharmacokinetics, tissue distribution and excretion study of 6-gingerol in rat by liquid chromatography-electrospray ionization time-of-flight mass spectrometry.
A rapid resolution liquid chromatography coupled with electrospray ionization (ESI) time-of-flight mass spectrometry method was developed and validated for quantitative analysis of 6-gingerol in plasma and various tissues. Liquid-liquid extraction was employed as sample preparation technique. Biological samples were separated on an Agilent Zorbax StableBond-C(18) column (4.6 mm x 50 mm, 1.8 microm) and detected by TOF/MS with electrospray ionization (ESI) interface in positive ion mode. Calibration curves (1/x(2) weighted) offered satisfactory linearity (r(2)>0.995) within the test range. The lower limit of quantification in different matrices was in a range of 10-100 ng/mL. Inter- and intra-day precision were in the range of 0.91-11.90% and 0.75-10.23%, respectively. Recoveries in plasma, urine and tissues ranged from 72.5% to 90.4%. Glucuronide of 6-gingerol, the major metabolite of 6-gingerol, was further determined after beta-glucuronidase hydrolyzation. This developed method was successfully applied to pharmacokinetics, tissue distribution and excretion studies of 6-gingerol after oral or intraperitoneal administration in rats.
Wang
Wei
W
Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing, China.
Li
Chang-Yin
CY
Wen
Xiao-Dong
XD
Li
Ping
P
Qi
Lian-Wen
LW
D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002240:Carbohydrate Sequence; D002396:Catechols / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005233:Fatty Alcohols / Q000493:pharmacokinetics* / Q000652:urine; D020719:Glucuronides / Q000652:urine; D007202:Indicators and Reagents; D007274:Injections, Intraperitoneal; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D014018:Tissue Distribution
D002396:Catechols; D005233:Fatty Alcohols; D020719:Glucuronides; D007202:Indicators and Reagents; C007845:gingerol