Chemically specific imaging of cryptosporidium oocysts using coherent anti-Stokes Raman scattering (CARS) microscopy.
We demonstrate the application of coherent anti-Stokes Raman scattering microscopy for the rapid, label-free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti-Stokes Raman scattering microscopy, demonstrating its capability for the rapid detection of cryptosporidium at the single oocyst level. We discuss the applicability of such a technique in a near-real time automated water testing system.
Murugkar
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School of Information Technology and Engineering (SITE), University of Ottawa, 800 King Edward, P.O. Box 450, Stn A, Ottawa, Ontario K1N6N5, Canada. murugkar@site.uottawa.ca
Evans
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Xie
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Anis
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D000818:Animals; D002417:Cattle; D003458:Cryptosporidium / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D004867:Equipment Design; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D033661:Oocysts / Q000648:ultrastructure*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods; D014867:Water / Q000469:parasitology*
D014867:Water