[Influence of glucagon-like peptide-2 on intestinal lymphocyte homing in mice with acute pancreatitis].
OBJECTIVE
To investigate the influence of glucagon-like peptide-2 (GLP-2) on intestinal lymphocyte homing receptor-integrin alpha 4 beta 7 and homing ligand-intestinal mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mice with acute pancreatitis (AP).


METHODS
A total of 96 mice were divided into three groups randomly (n=32 in each group): AP group, GLP-2 group and control group. Murine AP model was reproduced by intraperitoneal injection of caerulein and lipopolysaccharides (LPS). GLP-2 (250 microg/kg) was injected intraperitoneally at 15 minutes after the establishment of model, then it was injected twice a day for 3 days in GLP-2 group, while the mice in control group received normal saline instead. Mice were sacrificed at 6, 12, 24 and 48 hours after reproduction of AP, and tissue specimens were harvested. Integrin alpha 4 beta 7 positive peripheral blood lymphocytes were determined by flow cytometry. The expression of MAdCAM-1 in the terminal ileum mucosa and Peyer patch was measured by immunohistochemistry. Same observations were also done in the control and GLP-2 groups.


RESULTS
Compared with control group, integrin alpha 4 beta 7 positive lymphocyte in peripheral blood and the expression of MAdCAM-1 in the terminal ileum mucosa and Peyer patch were significantly reduced at 6, 12, 24 and 48 hours in AP mice (all P<0.05). Integrin alpha 4 beta 7 positive lymphocyte and the expression of MAdCAM-1 were markedly higher in GLP-2 group than those in AP group (all P<0.05), but were lower in GLP-2 group than those in control group (all P>0.05).


CONCLUSIONS
Administration of GLP-2 may restore expression of integrin alpha 4 beta 7 and MAdCAM-1, promote lymphocyte homing to intestine, thus improve the immunological function of intestine.
Kong
Ling-shang
LS
Department of General Surgery, the Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China.
Liu
Mu-lin
ML
Zhang
Zong-bing
ZB
Chen
De-li
DL
Liu
Rui-lin
RL
D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D053767:Glucagon-Like Peptide 2 / Q000494:pharmacology*; D007136:Immunoglobulins / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D009088:Mucoproteins / Q000378:metabolism; D010195:Pancreatitis / Q000276:immunology*; D011897:Random Allocation
D053767:Glucagon-Like Peptide 2; D007136:Immunoglobulins; D016023:Integrins; D009088:Mucoproteins; C071777:integrin alpha4beta7