TauT protects against cisplatin-induced acute kidney injury (AKI) established in a TauT transgenic mice model.
Cisplatin is a commonly used chemotherapeutic agent that has a major limitation because of its nephrotoxicity. Taurine is an important osmolyte that has been found to protect against cisplatin-induced apoptosis in renal cells in vitro. To determine whether over-expression of hTauT protects against cisplatin-induced acute kidney injury (AKI) in vivo, animals (wt and transgenic) were injected with cisplatin and the levels of BUN and serum creatinine were measured. Saline-injected mice were used as a control. The results showed that the levels of BUN and creatinine were significantly increased in the cisplatin-injected wild-type mice (110 +/- 12 mg/dl and 0.98 +/- 0.05 mg/dl) as compared to the saline-injected wild type mice (20 +/- 2.5 mg/dl and 0.52 +/- 0.06 mg/dl). However, over-expression of hTauT effectively prevented the progression of cisplatin-induced AKI in hTauT transgenic mice, as measured by the levels of BUN and serum creatininelevels (23 +/- 3.5 mg/dl and 0.6 +/- 0.05 mg/dl).
Han
Xiaobin
X
Department of Pediatrics, University of Tennessee Health Science Center and the Children's Memphis, TN, USA. xhan@utmem.edu
Chesney
Russell W
RW
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001483:Base Sequence; D001806:Blood Urea Nitrogen; D015153:Blotting, Western; D002945:Cisplatin / Q000633:toxicity*; D003404:Creatinine / Q000097:blood; D017931:DNA Primers; D005838:Genotype; D007668:Kidney / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D000099247:Taurine Transporters
D000970:Antineoplastic Agents; D002945:Cisplatin; D003404:Creatinine; D017931:DNA Primers; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D000099247:Taurine Transporters