title: Effect of biowaste sludge maturation on the diversity of thermophilic bacteria and archaea in an anaerobic reactor.
abstract: Prokaryotic diversity was investigated near the inlet and outlet of a plug-flow reactor. After analyzing 800 clones, 50 bacterial and 3 archaeal phylogenetic groups were defined. Clostridia (>92%) dominated among bacteria and Methanoculleus (>90%) among archaea. Significant changes in pH and volatile fatty acids did not invoke a major shift in the phylogenetic groups. We suggest that the environmental filter imposed by the saline conditions (20 g liter(-1)) selected a stable community of halotolerant and halophilic prokaryotes.
authors.affiliation: Universität Innsbruck, Institut für Mikrobiologie, Technikerstrasse 25 d, 6020, Innsbruck, Austria. marta.goberna@uibk.ac.at
authors.forename: M
authors.initials: M
authors.lastname: Goberna
authors.forename: H
authors.initials: H
authors.lastname: Insam
authors.forename: I H
authors.initials: IH
authors.lastname: Franke-Whittle
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005232:Fatty Acids, Volatile / Q000378:metabolism; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage / Q000382:microbiology*
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D012722:Sewage