Single step determination of PCB 126 and 153 in rat tissues by using solid phase microextraction/gas chromatography-mass spectrometry: Comparison with solid phase extraction and liquid/liquid extraction.
A simple and reliable solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) method was developed for the single-step determination of PCBs 126 and 153 in rat brain and serum, using liquid/liquid and solid phase extraction (SPE) as reference techniques. The multi-factor categorical experimental design used to study simultaneously the main parameters and their interactions affecting the efficiency of the method, showed that the use of an 85mum PA exposed at 100 degrees C for 40min was the optimum sampling condition for both PCBs. SPME was then validated by studying its linear dynamic (over two orders of magnitude), limits of detection (brain: 2ng/g, serum: 0.2ng/g) and analytical precision that was within 9% for SPME in both brain and serum. Finally, the method was used to determine the brain and blood target dose in mothers and pups after oral exposure of the mothers.
Poli
Diana
D
Laboratory of Industrial Toxicology, Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, via Gramsci 14, 43100 Parma, Italy.
Caglieri
Andrea
A
Goldoni
Matteo
M
Castoldi
Anna F
AF
Coccini
Teresa
T
Roda
Elisa
E
Vitalone
Annabella
A
Ceccatelli
Sandra
S
Mutti
Antonio
A
D000818:Animals; D001923:Brain Chemistry; D005591:Chemical Fractionation / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000097:blood / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D052617:Solid Phase Microextraction / Q000379:methods
D011078:Polychlorinated Biphenyls