title: C-type lectin LSECtin interacts with DC-SIGNR and is involved in hepatitis C virus binding.
abstract: Hepatitis C virus (HCV) is a major cause of liver disease. However, the detailed mechanism underlying hepatocyte infection with HCV is not yet completely understood. We previously identified a novel C-type lectin--LSECtin predominantly expressed on liver sinusoidal endothelial cells. Here we demonstrate that LSECtin can interact with two HCV receptors, DC-SIGNR and CD81, through its central ectodomain. Furthermore, cells expressing LSECtin specifically can be attached by the naturally occurring HCV in the sera of infected individuals. This binding was found to be mediated by the HCV E2 glycoprotein and could be efficiently inhibited by EGTA but not by mannan treatment. The present study suggests that LSECtin interaction with DC-SIGNR might contribute to HCV binding to liver sinusoidal endothelial cells.
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Bingtao
authors.initials: B
authors.lastname: Hao
authors.forename: Xuezhang
authors.initials: X
authors.lastname: Kuai
authors.forename: Guichun
authors.initials: G
authors.lastname: Xing
authors.forename: Juntao
authors.initials: J
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Zhang
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
mesh_terms: D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000378:metabolism; D016174:Hepacivirus / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus