[Construction of an acellular porcine aortic valve].
OBJECTIVE
To prepare a porcine aortic valve (PAV) free of the cellular components.


METHODS
The cellular components of porcine PAV were completely removed using trypsin and Triton X-100, and the acellular PAV was examined microscopically with HE staining with its physical and chemical properties assessed. Transmission electron microscopy was used to observe the integrity of the collagen and elastin and the DNA contents in the PAV was detected to confirm the total removal of the cellular components. With the fresh PAV as the control, small pieces of the acellular PAV were implanted into the subcutaneous tissues of 4 rabbits, and 4 weeks after the implantation, the implants were harvested for microscopic observation.


RESULTS
The cellular components were effectively removed from the cusps and roots of the PAV by trypsin and TritonX-100, with marked soluble protein loss [(0.24-/+0.04)% vs (0.48-/+0.12)%] and significantly increased water content [(92.2-/+1.5)% vs (89.2-/+1.6)%]. The acellular PAV still maintained good fibrous scaffold structure and the shrinkage temperature and tension at fracture underwent no significantly changes [(67.9-/+1.0) degrees celsius; vs (68.8-/+0.8) degrees celsius; and (489.3-/+19.0) g/mm2 vs (540.7-/+19.5) g/mm2, respectively]. The PAVs implanted in rabbits showed only mild tissue reaction with a few infiltrating neutrophils, lymphocytes and plasmocytes observed 4 weeks later. The accelular PAV caused obviously milder inflammatory reactions than fresh PAV.


CONCLUSIONS
The acellular PAV prepared by treatment with trypsin and Triton X-100 retains good fibrous scaffold structure and mechanical strength with low antigenicity.
Zhou
Hua-song
HS
Department of Cardiac Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. windzs115420@yahoo.com.cn
Gu
Chun-hu
CH
Chen
Wen-sheng
WS
Zhao
Jin-chao
JC
Wang
Yun-ya
YY
Tan
Hong-mei
HM
Yi
Ding-hua
DH
D000818:Animals; D001021:Aortic Valve / Q000166:cytology / Q000637:transplantation; D001705:Bioprosthesis; D002469:Cell Separation / Q000379:methods*; D017830:Octoxynol; D011474:Prosthesis Design; D011817:Rabbits; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
D017830:Octoxynol