Genetic variability and phylogeny of the high-risk HPV-31, -33, -35, -52, and -58 in central Brazil.
More than 100 HPV types have been described, 13 of which are classified as high-risk due to their association with the development of cervical cancer. The intratype genomic diversity of HPV-16 and -18 has been studied extensively, while little data have been generated for other less common high-risk types. The present study explores the nucleotide variability and phylogeny of the high-risk HPV-31, -33, -35, -52, and -58, in samples from Central Brazil. For this purpose, the LCR and the E6 and L1 genes were sequenced. Several variants of these HPV types were detected, some of which have been detected in other parts of the world. Furthermore, new variants of all types examined were characterized in a total of 13 new variants. Based on the E6 and L1 sequences, variants were described comprising conservative and non-conservative amino acid changes. For phylogenetic tree construction, samples characterized in this study were compared to others described and submitted to GenBank previously. The phylogenetic analysis of HPV-31, -33, -35, and -58 isolates did not reveal ethnic or geographical clustering as observed previously for HPV-16 and -18. HPV-35 analysis showed a dichotomic branching characteristic of viral subtypes. Interestingly, four clusters relative to the analysis of HPV-52 isolates were identified, two of which could be classified as Asian and European branches. The genomic characterization of HPV variants is crucial for understanding the intrinsic geographical relatedness and biological differences of these viruses and contributes further to studies on their infectivity and pathogenicity.
Raiol
Tainá
T
Department of Cellular Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.
Wyant
Patrícia Soares
PS
de Amorim
Regina Maria Santos
RM
Cerqueira
Daniela Marreco
DM
Milanezi
Natália von Gal
Nv
Brígido
Marcelo de Macedo
Mde M
Sichero
Laura
L
Martins
Cláudia Renata Fernandes
CR
D001938:Brazil / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D010802:Phylogeny; D012306:Risk; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral