title: Role of glyceraldehyde-3-phosphate dehydrogenase binding to hepatitis B virus posttranscriptional regulatory element in regulating expression of HBV surface antigen.
abstract: The hepatitis B virus (HBV) posttranscriptional regulatory element (HPRE) has been demonstrated to facilitate the cytoplasmic localization of unspliced transcripts. One cellular protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), is known to combine with this element. However, its function on HPRE remains unclear. Here, we show that recombinant GAPDH protein binds to HPRE RNA in vitro in streptavidin pull-down assays. Functional analysis demonstrated that GAPDH inhibited HPRE function in a pDM138-HPRE chloramphenicol acetyltransferase reporter assay system. Overexpression of GAPDH depressed the expression of HBs antigen, as detected both in cells transiently expressing HBs-HPRE and in HepG2.2.15 cells. These data indicate that GAPDH may be involved in the posttranscriptional regulation of HBV, which is critical for the life cycle of HBV.
authors.affiliation: Key Laboratory of Molecular Infectious Diseases, Ministry of Education, Chongqing Medical University, 1 Yi Xue Yuan Road, 400016, Chongqing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wan
authors.forename: Jieli
authors.initials: J
authors.lastname: Hu
authors.forename: Ailong
authors.initials: A
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D017930:Genes, Reporter; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*
chemical_list: D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; D012367:RNA, Viral; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D015500:Chloramphenicol O-Acetyltransferase