Exogenous N-acetylglucosamine increases hyaluronan production in cultured human dermal fibroblasts.
Application of hyaluronan (HA) containing cosmetic products to the skin is reported to moisturize and restore elasticity thereby achieving an antiwrinkle effect. In the skin, HA can be synthesized by dermal fibroblasts and N-acetylglucosamine (NAG) is a precursor for HA biosynthesis in the body. To study the effects of exogenous NAG on HA production in human dermal fibroblasts, HA production and HA-synthesizing enzymes 1, 2 and 3 mRNA expression in cultured human dermal fibroblasts were measured by ELISA and RT-PCR, respectively. The results showed that NAG promoted HA production while had no effect on the expression of HA-synthesizing enzymes 1, 2 and 3 mRNA in human dermal fibroblasts.
Tu
Cai-Xia
CX
Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, 116027 Dalian, People's Republic of China. tucx2003@163.com
Zhang
Rong-Xin
RX
Zhang
Xin-Jun
XJ
Huang
Tian
T
D000117:Acetylglucosamine / Q000494:pharmacology*; D002478:Cells, Cultured; D003358:Cosmetics; D020405:Dermis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D003358:Cosmetics; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; C523794:HAS1 protein, human; C101754:HAS2 protein, human; C105458:HAS3 protein, human; D000076002:Hyaluronan Synthases; D000117:Acetylglucosamine