title: Delayed lung maturation of foetus of diabetic mother rats develop with a diminish, but without changes in the proportion of type I and II pneumocytes, and decreased expression of protein D-associated surfactant factor.
abstract: Newborn children of diabetic mothers have an increased morbidity and mortality because of respiratory distress syndrome. We study lung histogenesis during intrauterine development of offspring of diabetic Sprague-Dawley rats at 18, 19 and 21 days of gestation (DG). Pregnant rats were grouped into diabetic (streptozotocin-induced), citrate, and control groups; five female and five male offspring were selected randomly from each group at 18, 19 and 21 DG, and a biopsy of the lung was taken and processed in paraffin for histological examination. The biopsy for the transmission electron microscopy (TEM) analysis was taken at 21 days. A delay in alveolization of the offspring at 18, 19 and 21 days of the diabetic group was observed, which was confirmed at TEM level, and also less quantity of protein D associated to surfactant in diabetic group was detected (P < 0.001). The foetuses of the diabetic group presented a delay in lung histogenesis and in differentiation of the type II pneumocytes cells, but conserved the proportion with a decrease in 50% of pneumocytes, accompanied by a diminish of protein D associated to surfactant factor.
authors.affiliation: Department of Basic Science, Health Science Division, University of Monterrey, Nuevo León, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Treviño-Alanís
authors.forename: J
authors.initials: J
authors.lastname: Ventura-Juárez
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Piñero
authors.forename: A
authors.initials: A
authors.lastname: Nevárez-Garza
authors.forename: A
authors.initials: A
authors.lastname: Quintanar-Stephano
authors.forename: A
authors.initials: A
authors.lastname: González-Piña
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000196:embryology* / Q000503:physiopathology; D005260:Female; D005325:Fetal Organ Maturity / Q000502:physiology*; D005865:Gestational Age; D008168:Lung / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D011247:Pregnancy; D011254:Pregnancy in Diabetics; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants