Expression of transcription factor Oct4 in bladder cancer cell line T24 and its effects on the biological characteristics of the cells.
The expression of octamer binding factor 4 (Oct4) gene in bladder cancer cell line T24 and its effects on the biological characteristics of the cells were investigated. RT-PCR and Western blot were employed to detect the expression of Oct4 in T24 cells. The changes of biological characteristics in T24 cells were analyzed before and after gene-silencing by Boyden chamber and MTT. The results showed that the expression of Oct4 gene was detectable in T24 cells by RT-PCR and Western blot. The expression of Oct4 gene and protein was down-regulated by siRNA, and average number of transwell cells in interference group, negative control group and blank control group was 101.40+/-4.56, 104.20+/-10.03 and 111.00+/-11.90, respectively. There was significant difference in the proliferation ability of the cells from 48 h, 72 h to 96 h after the interference by siRNA between interference group and negative group or blank control group (P<0.05). It was suggested that Oct4 gene was related with proliferation ability of T24 cells, but not with invasive capability.
Zhu
Zhiqiang
Z
Department of Emergency Center and Pathology, The First Hospital of Zhengzhou University, Institute of Clinical Medical Research of Henan Universities, Zhengzhou, China. zdzhuzhiqiang@126.com
Wen
Jianguo
J
Zheng
Xiangyu
X
Wang
Daoxie
D
Wang
Qingwei
Q
Fan
Changhui
C
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D034741:RNA, Small Interfering