title: Representational Difference Analysis (RDA) of bacteriophage genomes.
abstract: We implemented the Representational Difference Analysis (RDA) screening method to identify genome variations between related bacteriophages without the need for complete genome sequencing. The strategy, optimized on phiKMV and LKD16 and further evaluated on the newly isolated phage LUZ19, is based on three successive rounds of reciprocal RDA, with an increasing driver/tester molar ratio from 100/1 to 750/1. Using three relevant restriction endonucleases, only 4 to 6 sequences per restriction enzyme are necessary to provide sufficient discriminatory information to reveal the major genome variations between phages.
authors.affiliation: Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Elke
authors.initials: E
authors.lastname: Lammens
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Ceyssens
authors.forename: Marleen
authors.initials: M
authors.lastname: Voet
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hertveldt
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.forename: Guido
authors.initials: G
authors.lastname: Volckaert
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D003198:Computer Simulation; D016679:Genome, Viral; D023281:Genomics / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*