title: Absence of nasal blockage in a Japanese cedar pollen-induced allergic rhinitis model mouse.
abstract: BACKGROUND
  Japanese cedar pollen-induced allergic rhinitis in a guinea pig model clearly induced not only sneezing but also biphasic nasal blockage. To date, there have only been a few reports on models of murine allergic rhinitis which clearly show nasal blockage. Therefore, in order to try and develop such a model, we administered multiple dosages of intranasal pollen or purified antigen protein Cry j 1.
  
  
  METHODS
  B10.S mice were sensitized by intranasal instillations of either pollen extract or Cry j 1 twice a day for 7 days, which was adsorbed on Al(OH)(3). Subsequently, once a week, the mice were given multiple intranasal instillation challenges of either the pollen suspension or Cry j 1 and the frequency of sneezing was observed after respective challenges were made. Specific airway resistance (sRaw) was measured as an indicator for nasal blockage. Cry j 1-specific IgE levels were measured using an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The serum Cry j 1-specific IgE level showed clear elevation only in the group sensitized by Cry j 1 + Al(OH)(3) and then challenged by Cry j 1. No elevations were seen in the groups sensitized by pollen extract + Al(OH)(3) followed by a pollen suspension challenge. There was an immediate increase in sneezing after challenges in all of the sensitized-challenged groups. Nevertheless, no increases in sRaw in any of the groups were detected at any of the time points during the 8 hours following the challenges.
  
  
  CONCLUSIONS
  Cry j 1 may be more effective than crude antigens for efficient sensitization/challenge in mice. No increase in sRaw occurred, even in mice that possessed high amounts of Cry j 1-specific IgE and that exhibited sneezing.
authors.affiliation: Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ogita-Nakanishi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nabe
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Mizutani
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujii
authors.forename: Shigekatsu
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000403:Airway Resistance / Q000276:immunology; D000485:Allergens / Q000276:immunology; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology; D032485:Cryptomeria / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D015508:Nasal Obstruction / Q000276:immunology*; D010936:Plant Extracts / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000737:chemistry / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D012912:Sneezing / Q000276:immunology; D014611:Vaccination
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D010936:Plant Extracts; D010940:Plant Proteins; D007073:Immunoglobulin E