title: [Changes of NO2-/NO3- and nitration tyrosine concentrations in induced sputum of silicosis patients].
abstract: OBJECTIVE
  To analyze the change in nitration tyrosine, NO(2)(-)/NO(3)(-)level in induced sputum of silicosis patients and dust exposure workers and to evaluate the approach and feasibility of nitric oxide (NO) metabolites as early detection indicators of silicosis.
  
  
  METHODS
  Nitration tyrosine, NO(2)(-)/NO(3)(-)concentration in induced sputum of 80 dust exposure workers, 84 silicosis patients, 30 logistic personnel with no history of exposure to silica dust were determined and the relationship among Nitration tyrosine, NO(2)(-)/NO(3)(-)level and dust exposure years as well as pulmonary function tests were analyzed.
  
  
  RESULTS
  NO(2)(-)/NO(3)(-)level among exposed group [60.30 (46.58) micromol/l] was significantly higher than the control group [36.90 (22.28) micromol/l], (P < 0.05), and the level of NO(2)(-)/NO(3)(-)among the cases [79.65 (89.10) micromol/l] was significantly higher than exposed group as well as the control group (P < 0.05). Compared with control, the level of nitration tyrosine in exposed group [3.51 (0.46) nmol/l] and the cases [3.48 (0.49) nmol/l] was significantly higher (P < 0.05). NO(2)(-)/NO(3)(-)level and dust exposure years were positively correlated (r = 0.3733 and 0.3830 respectively P < 0.05); NO(2)(-)/NO(3)(-)level and pulmonary function tests (FVC%, FEV1.0%, PEF%, MEF25%, MEF50%) were negatively correlated (r = 0.1540, 0.1723, 0.1535, 0.1485, 0.1643 respectively, P < 0.05). There was no correlation between nitration tyrosine and dust exposure years (P > 0.05), no correlation between nitration tyrosine and pulmonary function test (P > 0.05).
  
  
  CONCLUSIONS
  The level of NO(2)(-)/NO(3)(-)level in induced sputum has a positive correlation with exposure to dust, suggesting that there will be a certain feasibility of the NO(2)(-)/NO(3)(-)as indicators of early detection of silicosis.
authors.affiliation: Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Liu
authors.forename: Jin-long
authors.initials: JL
authors.lastname: Men
authors.forename: En-guang
authors.initials: EG
authors.lastname: Wan
authors.forename: Ling-min
authors.initials: LM
authors.lastname: Kong
authors.forename: Jin-peng
authors.initials: JP
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Shen
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D006801:Humans; D008875:Middle Aged; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D012829:Silicosis / Q000378:metabolism*; D013183:Sputum / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D009566:Nitrates; D009573:Nitrites; D014443:Tyrosine