title: In vitro derby imaging of cancer biomarkers using quantum dots.
abstract: Semiconductor quantum dots (QDs), which have broad absorption with narrow emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and the arginine-glycine-aspartic acid (targeting the integrin alpha(v)beta(3)) in cancer cells is reported. Simultaneous fluorescence imaging of cellular distribution of nucleolin and integrin alpha(v)beta(3) using QDs enables easy monitoring of separate targets in the cancer cells and the normal healthy cells. These results suggest the feasibility of a concurrent visualization of QD-based multiple cancer biomarkers using small molecules such as aptamer or peptide ligands.
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Yongon-dong, Jongno-gu, Republic of Korea.
authors.forename: Mee Hyang
authors.initials: MH
authors.lastname: Ko
authors.forename: Soonhag
authors.initials: S
authors.lastname: Kim
authors.forename: Won Jun
authors.initials: WJ
authors.lastname: Kang
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyungu
authors.initials: H
authors.lastname: Kang
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Moon
authors.forename: Do Won
authors.initials: DW
authors.lastname: Hwang
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Ko
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
mesh_terms: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D018613:Microscopy, Confocal; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D045663:Quantum Dots; D016601:RNA-Binding Proteins / Q000378:metabolism; D000097796:Nucleolin
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D039302:Integrin alphaVbeta3; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C513936:AGRO 100; C047981:arginyl-glycyl-aspartic acid