title: Pluripotency of mouse spermatogonial stem cells maintained by IGF-1- dependent pathway.
abstract: Recent studies indicate that neonatal spermatogonial stem cells (SSCs) possess pluripotency. However, the mechanisms that regulate the pluripotent differentiation capacity of SSCs remain unclear. Here, we describe a new method to clonally derive pluripotent SSCs from neonatal mouse testis. By coculturing with testicular stromal cells, SSCs can be maintained and expanded in serum-free conditions. Unlike endogenous SSCs, these in vitro expanded SSCs showed strong alkaline phosphatase (AP) activity and displayed characteristics of embryonic stem cells and primordial germ cells, which were therefore designated as AP(+) germline stem cells (AP(+)GSCs). The pluripotency of AP(+)GSCs was confirmed by in vitro differentiation toward hepatic and neuronal lineages and formation of embryonic chimeras after injection into blastocysts. Further investigation revealed that insulin-like growth factor-1 (IGF-1) secreted from Leydig cells was a key factor involved in maintaining the pluripotency of AP(+)GSCs. The blockage of IGF-1 receptor phosphorylation and its downstream PI3K pathway by PPP or LY294002 dramatically reduced their AP activity and expression of pluripotent genes, such as Oct-4, Blimp1, and Nanog. In conclusion, the present study demonstrated that IGF-1 secreted by testicular Leydig cells plays an important role in maintaining the pluripotency of SSCs in culture, which provides an insight into the molecular mechanism underlying germ cell pluripotency.
authors.affiliation: Department of Biochemistry, Graduate Institute of Medical Sciences, School of Medicine, Taipei Medical University, Taipei, Taiwan. rita1204@tmu.edu.tw
authors.forename: Yen-Hua
authors.initials: YH
authors.lastname: Huang
authors.forename: Cheng-Chieh
authors.initials: CC
authors.lastname: Chin
authors.forename: Hong-Nerng
authors.initials: HN
authors.lastname: Ho
authors.forename: Chuan-Kai
authors.initials: CK
authors.lastname: Chou
authors.forename: Chia-Ning
authors.initials: CN
authors.lastname: Shen
authors.forename: Hung-Chih
authors.initials: HC
authors.lastname: Kuo
authors.forename: Tsai-Jung
authors.initials: TJ
authors.lastname: Wu
authors.forename: Yu-Chih
authors.initials: YC
authors.lastname: Wu
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Hung
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chang
authors.forename: Thai-Yen
authors.initials: TY
authors.lastname: Ling
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019070:Cell Lineage; D007334:Insulin-Like Growth Factor I / Q000378:metabolism* / Q000502:physiology; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D051379:Mice; D019899:Paracrine Communication; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013093:Spermatogonia / Q000166:cytology*; D013737:Testis / Q000166:cytology
chemical_list: D007334:Insulin-Like Growth Factor I