title: Use of two anesthetic combinations for semen collection by electroejaculation from captive coatis (Nasua nasua).
abstract: The objective was to compare the effects of ketamine-xylazine or tiletamine-zolazepam combinations as anesthetic protocols for captive coatis (Nasua nasua) for semen collection by electroejaculation. Five mature male coatis were physically restrained and then anesthetized by im injections of ketamine (10mg/kg) plus xylazine (1mg/kg) or a tiletamine-zolazepam combination (8 mg/kg). For the two combinations, additional quarter-doses of ketamine or the tiletamine-zolazepam combination were administered when necessary. Semen was collected by electroejaculation and immediately evaluated for color, volume, pH, sperm motility, vigor, morphology, acrosomal integrity, and percentage of live cells. Overall, collection of nine ejaculates was attempted from five animals for each treatment. Regardless of the anesthetic combination, all animals developed an erection during each attempt to collect semen. Ejaculates were obtained in all (9 of 9) attempts that used ketamine-xylazine for anesthesia, but in only 3 of 9 attempts (P<0.05) when tiletamine-zolazepam was used. All ejaculates contained sperm, with no significant differences in semen characteristics between the two anesthetic combinations. Recovery was smooth in all animals. In conclusion, semen collection by electroejaculation in coatis was significantly more successful with the use of a ketamine-xylazine combination than with tiletamine-zolazepam.
authors.affiliation: Department of Animal Science, Country Federal University of Semi-Arid - UFERSA, BR 110, Km 47, Presidente Costa e Silva, 59625-900, Mossoró, RN, Brazil.
authors.forename: F F P C
authors.initials: FF
authors.lastname: Barros
authors.forename: J P A F
authors.initials: JP
authors.lastname: Queiroz
authors.forename: A C M
authors.initials: AC
authors.lastname: Filho
authors.forename: E A A
authors.initials: EA
authors.lastname: Santos
authors.forename: V V
authors.initials: VV
authors.lastname: Paula
authors.forename: C I A
authors.initials: CI
authors.lastname: Freitas
authors.forename: A R
authors.initials: AR
authors.lastname: Silva
mesh_terms: D019162:Anesthetics, Combined / Q000494:pharmacology*; D000818:Animals; D000835:Animals, Wild / Q000502:physiology; D004338:Drug Combinations; D004542:Ejaculation / Q000187:drug effects / Q000502:physiology*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D045988:Procyonidae / Q000502:physiology; D012149:Restraint, Physical / Q000662:veterinary; D053625:Sperm Retrieval / Q000662:veterinary; D013992:Tiletamine / Q000494:pharmacology; D014991:Xylazine / Q000494:pharmacology; D015041:Zolazepam / Q000494:pharmacology
chemical_list: D019162:Anesthetics, Combined; D004338:Drug Combinations; D014991:Xylazine; D013992:Tiletamine; D007649:Ketamine; D015041:Zolazepam