title: ChE, GST and CAT: evaluation of the efficiency of a combined buffer for protein extraction.
abstract: Toxicity and biotransformation of several earthworm contaminants are widely evaluated nowadays using biochemical biomarkers. Many investigations track enzyme activities as biomarkers of neurotoxicity (cholinesterase (ChE)), metabolisation (glutathione-S-transferase (GST)) and oxidative stress (catalase (CAT)). This study proposes an evaluation of the use of a combined buffer, to extract proteins from earthworms and then analyse the 3 biomarkers. The method provides good results and allows protein extraction and quantitative determination of biomarkers with the same efficiency as the enzyme-specific buffers. It decreases preparation time and permits a study of the biomarkers on the same individual with only one homogenisation.
authors.affiliation: Laboratoire Chimie et Biochimie des Interactions, Université de Toulouse-Centre Universitaire JF Champollion, Albi, France. eva.schreck@univ-jfc.fr
authors.forename: E
authors.initials: E
authors.lastname: Schreck
authors.forename: F
authors.initials: F
authors.lastname: Geret
authors.forename: L
authors.initials: L
authors.lastname: Gontier
authors.forename: M
authors.initials: M
authors.lastname: Treilhou
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002021:Buffers; D002374:Catalase / Q000302:isolation & purification*; D002802:Cholinesterases / Q000302:isolation & purification*; D005982:Glutathione Transferase / Q000302:isolation & purification*; D007700:Kinetics; D009835:Oligochaeta / Q000187:drug effects / Q000201:enzymology*; D012989:Soil Pollutants / Q000633:toxicity; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D015415:Biomarkers; D002021:Buffers; D012989:Soil Pollutants; D014020:Tissue Extracts; D002374:Catalase; D005982:Glutathione Transferase; D002802:Cholinesterases