title: The flavonoid rutin but not the alkaloid arborinine induces apoptosis in a B-cell hybridoma cell line.
abstract: The effects of arborinine, an alkaloid extracted from Erthela bahiensis and of rutin, a flavonoid obtained from Dimorphandra mollis (Benth.), Brazilian medicinal plants, on the viability and function of a murine B-cell hybridoma as a tumor model were investigated. The flavonoid rutin at 50 microM induced an increase in the number of apoptotic cells of one- to fivefold and reductions in cellular proliferation and monoclonal antibody production. Less but still significant necrosis was also induced by rutin under the same experimental conditions. On the other hand, the alkaloid arborinine exerted no significant effects on the studied parameters.
authors.affiliation: Immunology and Molecular Laboratory, Federal University of Bahia, BA, Brazil. bino7@terra.com.br
authors.forename: Renata
authors.initials: R
authors.lastname: Roseghini
authors.forename: Georgenes Morato
authors.initials: GM
authors.lastname: Falcão
authors.forename: José Fernando
authors.initials: JF
authors.lastname: Oliveira Costa
authors.forename: Jorge
authors.initials: J
authors.lastname: Clarêncio
authors.forename: Ivana
authors.initials: I
authors.lastname: Nascimento
authors.forename: Eudes
authors.initials: E
authors.lastname: Velozo
authors.forename: Robert
authors.initials: R
authors.lastname: Schaer
authors.forename: Vera
authors.initials: V
authors.lastname: Vale
authors.forename: Silvia Lima
authors.initials: SL
authors.lastname: Costa
authors.forename: Maria de Fátima Dias
authors.initials: Mde F
authors.lastname: Costa
authors.forename: Marcienne
authors.initials: M
authors.lastname: Tardy
authors.forename: Roberto
authors.initials: R
authors.lastname: Meyer
authors.forename: Songelí
authors.initials: S
authors.lastname: Menezes Freire
mesh_terms: D000166:Acridines / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006825:Hybridomas / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009336:Necrosis / Q000139:chemically induced; D010936:Plant Extracts / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*; D012431:Rutin / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds
chemical_list: D000166:Acridines; D000911:Antibodies, Monoclonal; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C022784:arborinine; D012431:Rutin