title: Molecular identification of Mycobacterium chimaera as a cause of infection in a patient with chronic obstructive pulmonary disease.
abstract: This report describes a case of Mycobacterium chimaera infection in a patient with a history of chronic obstructive pulmonary disease where the organism was identified by using molecular methods. M. chimaera was identified from fresh lung tissue and from an instrument-negative mycobacterial growth indicator tube broth culture. The utility of using sequence analysis of the internal transcribed spacer region for the rapid identification of a slow-growing nontuberculous Mycobacterium spp. where conventional culture methods were not successful was shown.
authors.affiliation: i(3)Bio, LLC, Omaha, NE 68022, USA.
authors.forename: Nathan D
authors.initials: ND
authors.lastname: Bills
authors.forename: Steven H
authors.initials: SH
authors.lastname: Hinrichs
authors.forename: Tricia A
authors.initials: TA
authors.lastname: Aden
authors.forename: Robert S
authors.initials: RS
authors.lastname: Wickert
authors.forename: Peter C
authors.initials: PC
authors.lastname: Iwen
mesh_terms: D000368:Aged; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004864:Equipment and Supplies / Q000382:microbiology; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D029424:Pulmonary Disease, Chronic Obstructive / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer