Global proteomic analysis of the chromate response in Arthrobacter sp. strain FB24.
A global proteomic evaluation of the response of Arthrobacter sp. strain FB24 to 5 and 20 mM Cr(VI) was conducted using both two-dimensional gel electrophoresis (2-DGE) and liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS). The changes in protein expression found with 2-DGE indicate alterations in central metabolism and amino acid synthesis. Proteome coverage increased from 22% with 2-DGE to 71% with LC/LC-MS/MS. The proteins exhibiting the highest levels of expression under Cr(VI) stress suggest intracellular sulfur limitation, which could be driven by competition for the sulfate (SO4(2-)) transporter by the chromate (CrO4(2-)) ion. These results are consistent with the growth defects seen with strain FB24 when Cr(VI) concentrations exceeded 5 mM.
Henne
Kristene L
KL
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA. khenne@purdue.edu
Turse
Joshua E
JE
Nicora
Carrie D
CD
Lipton
Mary S
MS
Tollaksen
Sandra L
SL
Lindberg
Carl
C
Babnigg
Gyorgy
G
Giometti
Carol S
CS
Nakatsu
Cindy H
CH
Thompson
Dorothea K
DK
Konopka
Allan E
AE
D001173:Arthrobacter / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002840:Chromates / Q000494:pharmacology*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D002840:Chromates; D020543:Proteome