Sox17 facilitates the differentiation of mouse embryonic stem cells into primitive and definitive endoderm in vitro.
The Sox family of HMG (high mobility group)-box transcription factors are highly conserved in vertebrates. Sox members are involved in various developmental processes. Among them Sox17 has been demonstrated to function as an endoderm determinant in zebrafish and Xenopus, respectively. However, little is known about the role of Sox17 in mouse embryonic stem cell (ESC) differentiation. In our research, we investigated the effect of Sox17 on mouse ESC and embryoid body (EB) differentiation. The results demonstrated that Sox17 overexpression upregulated a set of endoderm-specific gene markers, suggesting that Sox17 overexpression induced an ESC differentiation program towards both primitive and definitive endoderm. We believe this finding brings new insights into the understanding of ESC differentiation and the organogenesis of endodermal derivatives.
Qu
Xue-Bin
XB
Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, 88 East Wenhua Road, Jinan City, Shandong Province, China.
Pan
Jie
J
Zhang
Cong
C
Huang
Shu-Yang
SY
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D019521:Body Patterning / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000502:physiology*; D004707:Endoderm / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D024002:HMGB Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D055760:SOXF Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
D015415:Biomarkers; D024002:HMGB Proteins; D055760:SOXF Transcription Factors; C511606:Sox17 protein, mouse