title: Minimum intravenous infectious dose of ovine progressive pneumonia virus (OPPV).
abstract: The minimum intravenous infectious dose for ovine progressive pneumonia virus (OPPV) WLC1 was determined using twenty-four 6month-old lambs. Twelve groups of two 6month-old lambs were inoculated intravenously (i.v.) with tissue culture fluid containing ovine progressive pneumonia virus (OPPV) WLC1 titers ranging from 10(7.6) TCID(50)/lamb down to 10(-3.4) TCID(50)/lamb and were monitored for seroconversion using the OPPV agar gel immunodiffusion assay (AGID). Fifteen of the 16 lambs given equal or greater than 10(0.6) TCID(50) seroconverted, and virus could be isolated from peripheral blood leukocytes in 13 out of the 15 of these lambs. None of the eight lambs receiving less than 10(0.6) TCID(50) seroconverted during the 12months. The results of this study indicated that 10(0.6) or 4 TCID(50)/lamb given i.v. was capable of establishing infection.
authors.affiliation: Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, United States. Iherrman@vetmed.wsu.edu
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Herrmann-Hoesing
authors.forename: Howard D
authors.initials: HD
authors.lastname: Lehmkuhl
authors.forename: Randall C
authors.initials: RC
authors.lastname: Cutlip
mesh_terms: D000375:Aging; D000818:Animals; D001168:Arthritis / Q000209:etiology / Q000662:veterinary / Q000821:virology; D003937:Diagnosis, Differential; D004197:Disease Reservoirs; D018562:Disease Transmission, Infectious / Q000662:veterinary; D005260:Female; D018111:Pneumovirus / Q000302:isolation & purification / Q000472:pathogenicity*; D018186:Pneumovirus Infections / Q000635:transmission / Q000662:veterinary*; D011247:Pregnancy; D012418:Ruminants / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D019562:Viral Load / Q000662:veterinary