title: Temephos-induced resistance in Aedes aegypti and its cross-resistance studies to certain insecticides from India.
abstract: A temephos-induced resistance in Aedes aegypti that was developed for 24 generations exhibited 20.3-fold resistance as compared to susceptible strain. The diagnostic dose of temephos 0.02 mg/l exhibited gradual decrease in larval mortality with the progression of generations. An operational dose (1 mg/l) exhibited the LT(50) value of 41.42 min in the susceptible population, whereas the value of the resistant population increased to 72.62, 108.86, 122.34, 182.03, 244.82 and 304.86 min in the fourth, eighth, 12th, 16th, 20th and 24th generation, respectively. The study carried out showed 120 min as the cut-off limit for differentiation between susceptible and resistant A. aegypti. Cross-resistance studies showed a varied degree of cross-resistance to fenthion, chlorpyrifos, malathion and DDT, whereas comparatively higher cross-resistance was observed to chlorpyrifos. Study on diagnostic doses of insecticides to A. aegypti females indicated a gradual decrease in adult mortality at every eighth generation as compared to susceptible population when exposed to malathion 0.8%, fenthion 0.25%, DDT 4%, permethrin 0.25% and temephos 6.5%, whereas little or no change in mortality when exposed to lambda-cyhalothrin 0.03% and propoxur 0.1%, was observed. Thus, the expression of larval resistance was observed in adult stages also.
authors.affiliation: Entomology Division, Defence Research and Development Establishment, Gwalior, India. sachin_ento@rediffmail.com
authors.forename: S N
authors.initials: SN
authors.lastname: Tikar
authors.forename: Arkaja
authors.initials: A
authors.lastname: Kumar
authors.forename: G B K S
authors.initials: GB
authors.lastname: Prasad
authors.forename: Shri
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D004390:Chlorpyrifos / Q000494:pharmacology; D003634:DDT / Q000494:pharmacology; D004351:Drug Resistance; D005284:Fenthion / Q000494:pharmacology; D007194:India; D007306:Insecticides / Q000494:pharmacology*; D008294:Malathion / Q000494:pharmacology; D016019:Survival Analysis; D000002:Temefos / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D007306:Insecticides; D005284:Fenthion; D003634:DDT; D004390:Chlorpyrifos; D000002:Temefos; D008294:Malathion