title: Identification of ellagic acid derivatives in methanolic extracts from Qualea species.
abstract: The methanolic extract from the barks of the medicinal plant Qualea parviflora (Vochysiaceae) was fractionated by column chromatography over silica gel followed by gel permeation over Sephadex LH-20 to give 3,3'-di-O-methylellagic acid-4-O-beta-D-glucopyranoside (1), 3-O-methylellagic acid-4'-O-alpha-L-rhamnopyranoside (2), 3,3',4-tri-O-methylellagic acid-4'-O-beta-D-glucopyranoside (3), and 3,3'-di-O-methylellagic acid (4), together with triterpenes and saponins. We also performed comparative analyses among this species and Q. grandiflora and Q. multiflora using high-pressure liquid chromatography. The biological assays showed that, when compared to the standard ellagic acid, compounds 1-4 are less cytotoxic but have a lower capacity of stimulating murine peritoneal macrophages to release nitric oxide and tumoural-alpha necrose factor.
authors.affiliation: UNESP, São Paulo State University, Instituto de Química de Araraquara, C. Postal 355, 14801-970, Araraquara, SP, Brazil. nasser@iq.unesp.br
authors.forename: Ana L M
authors.initials: AL
authors.lastname: Nasser
authors.forename: Camila B A
authors.initials: CB
authors.lastname: Carli
authors.forename: Clenilson M
authors.initials: CM
authors.lastname: Rodrigues
authors.forename: Danielle C G
authors.initials: DC
authors.lastname: Maia
authors.forename: Iracilda Z
authors.initials: IZ
authors.lastname: Carlos
authors.forename: Marcos N
authors.initials: MN
authors.lastname: Eberlin
authors.forename: Clélia A
authors.initials: CA
authors.lastname: Hiruma-Lima
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D000432:Methanol; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification*; D013864:Thioglycolates / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D013864:Thioglycolates; D014409:Tumor Necrosis Factor-alpha; D004610:Ellagic Acid; D009569:Nitric Oxide; D000432:Methanol