title: Metabolic shift in the phytopathogen Rhodococcus fascians in response to cell-free extract of infected tobacco plant tissues.
abstract: The phytopathogen Rhodococcus fascians induces the development of leafy gall, which is considered to be its ecological niche. To obtain a view of the metabolic changes occurring in R. fascians during this process, an in vitro system was used where bacteria are grown in the presence of a leafy gall extract, a condition mimicking that found by the bacteria in infected plants. Proteins of R. fascians grown for 24 h under these conditions were displayed by two-dimensional polyacrylamide gel electrophoresis. Fifteen polypeptides showing a differential accumulation in response to the inducing conditions were analyzed by mass spectrometry. Two polypeptides potentially linked to the Krebs cycle, a pyruvate dehydrogenase and a fumarate hydratase, were further characterized and shown to be downregulated at the transcriptional level. The identification of these two enzymes suggests that R. fascians may shift its metabolism during the interaction with plants from the Krebs cycle to the glyoxylate shunt.
authors.affiliation: Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue des Professeurs Jeener et Brachet 12, Gosselies, Belgium.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Forizs
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lestrade
authors.forename: Adeline
authors.initials: A
authors.lastname: Mol
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Dierick
authors.forename: Cécile
authors.initials: C
authors.lastname: Gerbaux
authors.forename: Billo
authors.initials: B
authors.lastname: Diallo
authors.forename: Mondher
authors.initials: M
authors.lastname: El Jaziri
authors.forename: Marie
authors.initials: M
authors.lastname: Baucher
authors.forename: Olivier M
authors.initials: OM
authors.lastname: Vandeputte
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000032:analysis; D012240:Rhodococcus / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000737:chemistry* / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010936:Plant Extracts; D020543:Proteome