title: Identification of mutation sites on Delta12 desaturase genes from Mortierella alpina 1S-4 mutants.
abstract: The mutation sites on the Delta12 desaturase gene in Mortierella alpina Delta12 desaturase-defective mutants SR88, TM912, and Mut48 accumulating Mead acid were identified. Each mutation resulted in an amino acid replacement (H116Y and P166L) in the Delta12 desaturase gene from SR88 and Mut48, respectively.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakuradani
authors.forename: Takahiro
authors.initials: T
authors.lastname: Abe
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumura
authors.forename: Akiko
authors.initials: A
authors.lastname: Tomi
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D044943:Fatty Acid Desaturases / Q000235:genetics*; D020094:Mortierella / Q000201:enzymology*; D009154:Mutation
chemical_list: D017931:DNA Primers; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase