[Isolation and study of antibiotic INA-1132 (chlorothricin), produced by actinomycete strain].
Taxonomic properties of strain INA-1132 producing antibiotic INA-1132 are described. The antibiotic showed activity against grampositive bacteria and fungi. The strain was classified as belonging to the genus Streptomyces and by its taxomic characteristics is most close to S. baarnensis. The experiments with the bacterial culture Halobacterium salinarum (previously H. halobium) revealed hypolipidemic activity of the antibiotic, i. e. its ability to inhibit biosynthesis of sterols. Conditions for the production of the antibiotic, methods of its isolation and purification, as well as the results of the chemical structure elucidation are described. By its physicochemical properties the antibiotic is identical to chlorothricin. The structure of antibiotic INA-1132 was ascertained by X-ray analysis. Conformation of the molecule of chlorothricin (antibiotic 1132) was determined for the first time.
Terekhova
L P
LP
Galatenko
O A
OA
Trenin
A S
AS
Tolstykh
I V
IV
Zenkova
V A
VA
Zhukhlistova
N E
NE
Ol'khovatova
O L
OL
Malkina
N D
ND
Boĭkova
Iu V
IuV
Ustinova
E V
EV
Katrukha
G S
GS
D039903:Actinobacteria / Q000378:metabolism*; D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D006094:Gram-Positive Bacteria / Q000187:drug effects; D019617:Halobacterium salinarum / Q000187:drug effects; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000494:pharmacology; D008968:Molecular Conformation; D013261:Sterols / Q000096:biosynthesis; D013302:Streptomyces / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000960:Hypolipidemic Agents; D013261:Sterols; C000451:chlorothricin