title: Effect of isoflavone extracts from Glycine max on human endothelial cell damage and on nitric oxide production.
abstract: OBJECTIVE
  In this study, we determined the protective effect of isoflavones from Glycine max on human umbilical vein endothelial cell (ECV304) damage induced by hydrogen peroxide (H2O2) and on nitric oxide (NO) production.
  
  
  METHODS
  We studied the regulation of NO synthesis in cultured human endothelial cells by phytoestrogens contained in soy extracts in the presence or absence of ICI 182,780 or N(omega)-nitro-L-arginine methyl esther and determined the protective effect of these isoflavones on ECV304 damage induced by H2O2.
  
  
  RESULTS
  We show that soy extracts activate NO synthesis in endothelial cells and protect against cell damage.
  
  
  CONCLUSIONS
  In conclusion, soy isoflavones markedly protect ECV304 cells against H2O2 damage and promote NO synthesizing. Therefore, these isoflavones can potentially act as an NO promoter and as an antioxidant.
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Michele
authors.initials: M
authors.lastname: Paulo
authors.forename: Mirian Mendonça
authors.initials: MM
authors.lastname: Salvador
authors.forename: Mário
authors.initials: M
authors.lastname: dos Anjos Neto Filho
authors.forename: Marlise Bonetti Agostinho
authors.initials: MB
authors.lastname: Montes
authors.forename: Silvio Antônio
authors.initials: SA
authors.lastname: Franceschini
authors.forename: Maria Regina Torqueti
authors.initials: MR
authors.lastname: Toloi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000187:drug effects; D047629:Estrogen Receptor beta / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D018384:Oxidative Stress / Q000187:drug effects*; D013025:Glycine max / Q000737:chemistry; D014471:Umbilical Veins
chemical_list: D000975:Antioxidants; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D007529:Isoflavones; D009569:Nitric Oxide; C004742:daidzein; D006861:Hydrogen Peroxide; D019833:Genistein