title: Effect of the proton motive force inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development.
abstract: OBJECTIVE
  Proton motive force (PMF) inhibition enhances the intracellular accumulation of autoinducers possibly interfering with biofilm formation. We evaluated the effect of the PMF inhibitor carbonyl cyanide-m-chlorophenylhydrazone (CCCP) on Pseudomonas aeruginosa biofilm development.
  
  
  RESULTS
  Four epidemiologically unrelated P. aeruginosa isolates were studied. A MexAB-oprM overproducing strain was used as control. Expression of gene mexB was examined and biofilm formation after incubation with 0, 125 and 25 micromol l(-1) of CCCP was investigated. Mean values of optical density were analysed with one-way analysis of variance and t-test. Two isolates subexpressed mexB gene and only 25 micromol l(-1) of CCCP affected biofilm formation. Biofilms of the other two isolates and control strain PA140 exhibited significantly lower absorbance (P ranging from < 001 to < 0.05) with either 12.5 or 25 micromol l(-1) of CCCP.
  
  
  CONCLUSIONS
  The PMF inhibitor CCCP effect was correlated with the expression of MexAB-OprM efflux system and found to compromise biofilm formation in P. aeruginosa.
  
  
  CONCLUSIONS
  These data suggest that inhibition of PMF-dependent trasporters might decrease biofilm formation in P. aeruginosa.
authors.affiliation: Department of Microbiology, University Hospital of Larissa, Larissa, Greece. ado@med.uth.gr
authors.forename: A
authors.initials: A
authors.lastname: Ikonomidis
authors.forename: A
authors.initials: A
authors.lastname: Tsakris
authors.forename: M
authors.initials: M
authors.lastname: Kanellopoulou
authors.forename: A N
authors.initials: AN
authors.lastname: Maniatis
authors.forename: S
authors.initials: S
authors.lastname: Pournaras
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000187:drug effects*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006801:Humans; D018892:Proton-Motive Force; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development*; D014475:Uncoupling Agents / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone