[Proteinchip-flight mass spectrometry for screening differentially expressed proteins in the serum of cervical cancer patients].
OBJECTIVE
To identify the differentially expressed proteins in the serum of patients with cervical cancer for use as the biomarkers for early diagnosis of cervical cancer.


METHODS
Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) with weak cationic chips (CM10) was used to examine the serum samples of 24 patients with cervical squamous cell carcinoma and 25 age-matched healthy women. The protein fingerprints were obtained, and bioinformatic analysis was performed to identify the differentially expressed proteins in the serum of the patients.


RESULTS
Fifty-two differentially expressed proteins were detected in the serum of cervical cancer patients (P<10(-5)), among which 6 proteins with mass/charge ratio of 4173.77, 5903.09, 6087.12, 10716.9, 6109.61 and 3397.41, respectively, showed lowered expression in the serum of cervical cancer patients. Two diagnostic models for cervical cancer were generated using software, including one consisting of the 4173.77(M/Z) protein with the diagnostic specificity of 96% and sensitivity of 75% for cervical cancer and the other consisting of 3 proteins at 5335.81(M/Z), 7562.99(M/Z), and 9287.89(M/Z) with specificity of 91.67% and sensitivity of 96.0%.


CONCLUSIONS
Cervical cancer patients show different serum protein expression profile from healthy women. The 6 differential proteins identified may serve as specific serum biomarkers in close relation to the origin and progression of cervical cancer.
Cai
Si-na
SN
Department Of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. caisina2224@163.com
Liu
Guo-bing
GB
Guo
Xiao-hong
XH
Chen
Jian-guang
JG
Zhang
Jun
J
Wu
Yi-long
YL
Liang
Wei-jiang
WJ
Luo
Rong-cheng
RC
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000175:diagnosis
D014408:Biomarkers, Tumor; D001798:Blood Proteins