title: Cellular response of preosteoblasts to nanograined/ultrafine-grained structures.
abstract: Metallic materials with submicron- to nanometer-sized grains provide surfaces that are different from conventional polycrystalline materials because of the large proportion of grain boundaries with high free energy. In the study described here, the combination of cellular and molecular biology, materials science and engineering advances our understanding of cell-substrate interactions, especially the cellular activity between preosteoblasts and nanostructured metallic surfaces. Experiments on the effect of nano-/ultrafine grains have shown that cell attachment, proliferation, viability, morphology and spread are favorably modulated and significantly different from conventional coarse-grained structures. Additionally, immunofluorescence studies demonstrated stronger vinculin signals associated with actin stress fibers in the outer regions of the cells and cellular extensions on nanograined/ultrafine-grained substrate. These observations suggest enhanced cell-substrate interaction and activity. The differences in the cellular response on nanograined/ultrafine-grained and coarse-grained substrates are attributed to grain size and degree of hydrophilicity. The outcomes of the study are expected to reduce challenges to engineer bulk nanostructured materials with specific physical and surface properties for medical devices with improved cellular attachment and response. The data lay the foundation for a new branch of nanostructured materials for biomedical applications.
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Center for Structural and Functional Materials, University of Louisiana at Lafayette, P.O. Box 44130, Lafayette, LA 70504, USA. dmisra@louisiana.edu
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
authors.forename: W W
authors.initials: WW
authors.lastname: Thein-Han
authors.forename: T C
authors.initials: TC
authors.lastname: Pesacreta
authors.forename: K H
authors.initials: KH
authors.lastname: Hasenstein
authors.forename: M C
authors.initials: MC
authors.lastname: Somani
authors.forename: L P
authors.initials: LP
authors.lastname: Karjalainen
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D011506:Proteins / Q000378:metabolism; D013193:Stainless Steel / Q000737:chemistry; D022502:Stress Fibers / Q000378:metabolism; D013499:Surface Properties; D013997:Time Factors
chemical_list: D011506:Proteins; D013193:Stainless Steel