title: [A method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles].
abstract: OBJECTIVE
  To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.
  
  
  METHODS
  Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis.
  
  
  RESULTS
  There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05).
  
  
  CONCLUSIONS
  It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.
authors.affiliation: Institute of Forensic Science, Chongqing Public Security Bureau, Chongqing 400042, China. fy777777@126.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Feng
authors.forename: Mi
authors.initials: M
authors.lastname: Yan
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
mesh_terms: D004247:DNA / Q000032:analysis*; D004847:Epithelial Cells / Q000378:metabolism*; D053803:Forensic Genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D009061:Mouth Mucosa / Q000166:cytology*; D016133:Polymerase Chain Reaction; D014099:Toothbrushing
chemical_list: D004247:DNA