[Detection of RNA expression on whole genome analysis of Mycobacterium leprae by tiling array].
Completion of Mycobacterium leprae genome sequence revealed that there are many pseudogenes and non-coding regions, but rather small numbers of protein-coding genes. Although it was thought that pseudogenes and non-coding regions were silent and junk, our previous studies indicated that RNA expression was detected from these regions. To elucidate comprehensive RNA expression pattern on M. leprae whole genome, tiling array was designed and total RNA of M. leprae Thai-53 strain was analyzed. As a result, highly expressed regions were detected among not only the gene regions but also pseudogenes and non-coding regions. Since some of the RNA expression levels were modulated by MDT, evaluation of RNA expression pattern might be a good indicator for the treatment of leprosy.
Akama
Takeshi
T
Department of Bioregulation, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama, Tokyo 189-0002, Japan. akama@nih.go.jp
Suzuki
Koichi
K
Tanigawa
Kazunari
K
Kawashima
Akira
A
Wu
Huhehasi
H
Nakamura
Kazuaki
K
Hayashi
Moyuru
M
Ishii
Norihisa
N
D015870:Gene Expression; D016680:Genome, Bacterial / Q000235:genetics*; D009166:Mycobacterium leprae / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011544:Pseudogenes / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics*
D012329:RNA, Bacterial