In vitro osteogenic differentiation of cultured human dental papilla-derived cells.
OBJECTIVE
This study examined the osteogenic phenotypes and mineralization of cultured human dental papilla-derived cells.


METHODS
Dental papillae were harvested from mandibles during surgical extraction of lower impacted third molars from 3 patients aged 13 to 15 years. The dental papilla-derived cells were introduced into the cell culture. After passage 3, the dental papilla-derived cells were further cultured for 42 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. We examined the histochemical detection of alkaline phosphatase (ALP), the reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for ALP and osteocalcin, and von Kossa staining in the dental papilla-derived cells.


RESULTS
It was observed that ALP was strongly expressed in the earlier stage of osteoblastic differentiation, whereas osteocalcin was mainly expressed and secreted into the medium at the later stage. Von Kossa-positive mineralization nodules were first observed on day 14, which increased in number during the entire culture period.


CONCLUSIONS
These results suggest that dental papilla-derived cell have osteogenic potential and could be used as an additional source of cells for bone tissue engineering.
Park
Bong-Wook
BW
Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Gyeongsangnam-do, South Korea.
Hah
Young-Sool
YS
Choi
Mun-Jeoung
MJ
Ryu
Young-Mo
YM
Lee
Sung-Gyoon
SG
Kim
Deok Ryong
DR
Kim
Jong-Ryoul
JR
Byun
June-Ho
JH
D000293:Adolescent; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002113:Calcification, Physiologic / Q000235:genetics*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008964:Molar, Third / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*
D015675:Osteocalcin; D000469:Alkaline Phosphatase