Production of chromopyrrolic acid by coexpression of inkOD in a heterologous host Streptomyces albus.
Two ink genes, inkO and inkD, responsible for the earliest steps of K252a biosynthetic pathway, from Nonomurea longicantena JCM 11136 were heterologously coexpressed in Streptomycesalbus J1074. The resultant strain accumulated compound that was purified by HPLC and studied by NMR. Coexpression of inkOD yielded chromopyrrolic acid, the key intermediate in an indolocarbazole biosynthesis.
Chae
Choong-Sik
CS
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
Park
Jin-Soo
JS
Chung
Soon-Chun
SC
Kim
Tae-Im
TI
Lee
So-Hyoung
SH
Yoon
Kyung-Mi
KM
Shin
Jongheon
J
Oh
Ki-Bong
KB
D002227:Carbazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D015195:Drug Design; D026121:Indole Alkaloids / Q000138:chemical synthesis / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D005810:Multigene Family; D011758:Pyrroles / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry
D002227:Carbazoles; D026121:Indole Alkaloids; D011758:Pyrroles; C541708:chromopyrrolic acid; D014364:Tryptophan; C049985:staurosporine aglycone