Mass-mapping of ECM macromolecules by scanning transmission electron microscopy.
In the scanning transmission electron microscope, the degree of electron scattering induced by biological specimens, such as ECM macromolecules, is dependent on the molecular mass. By calibrating the ratio of scattered to non-scattered electrons against a known mass standard, such as tobacco mosaic virus, it is possible to quantify absolute changes in both mass and mass distribution. These mass mapping approaches can provide important information on ECM assembly, organisation, and interactions which is not obtainable by other means.
Sherratt
Michael J
MJ
The University of Manchester, Manchester, M13 9PT, UK. michael.j.sherratt@manchester.ac.uk
Graham
Helen K
HK
Kielty
Cay M
CM
Holmes
David F
DF
D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods*; D008970:Molecular Weight
D016326:Extracellular Matrix Proteins