title: Phasic and tonic modes of depolarization-exocytosis coupling in beta-cells of porcine islets of Langerhans.
abstract: In response to depolarizations that open voltage dependent Ca2+ channels single porcine beta-cells display heterogeneous time courses of exocytosis. Some cells display phasic exocytosis that is triggered by individual or short burst of action potentials typically characteristic of glucose-induced electrical activity or brief voltage clamp depolarization. Other cells, singularly or additionally, display tonic exocytosis that (i) is triggered during prolonged (up to seconds-long) depolarizations to voltages (-30 to -20 mV), and (ii) coincides with rises in global cytosolic [Ca2+] >500 nM. We suggest that tonic exocytosis (i) likely results from a recently described pool of granules that is more Ca2+ sensitive and less co-localized with voltage-sensitive Ca2+ entry channels than that contributing to phasic exocytosis and (ii) helps tune exocytosis to glucose-induced electrical activity when the latter consists of spike activity followed by intervals of plateau depolarization to nearly -20 mV.
authors.affiliation: Departments of Internal Medicine & Cell Biology/Physiology, Washington University Medical Center, Saint Louis, MO 63110, USA.
authors.forename: Stanley
authors.initials: S
authors.lastname: Misler
authors.forename: Amelia M
authors.initials: AM
authors.lastname: Silva
authors.forename: David
authors.initials: D
authors.lastname: Barnett
authors.forename: Adam S
authors.initials: AS
authors.lastname: Dickey
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology; D004594:Electrophysiology; D005089:Exocytosis / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D050417:Insulin-Secreting Cells / Q000502:physiology*; D007515:Islets of Langerhans / Q000166:cytology*; D008564:Membrane Potentials / Q000502:physiology*; D013552:Swine
chemical_list: D015220:Calcium Channels; D005947:Glucose; D002118:Calcium