Analgesic and anti-inflammatory activities of Torenia concolor Lindley var. formosana Yamazaki and betulin in mice.
The present study was intended to examine the analgesic effect of the 70% methanol extract of Torenia concolor Lindley var. formosana Yamazaki (TC(MeOH)) and betulin using models of acetic acid-induced writhing response and formalin test. In addition, we investigated the anti-inflammatory effect of TC(MeOH) and betulin using model of lambda-carrageenan-induced paw edema. We observed the activities of antioxidant enzymes (SOD, GPx and GR) in the liver and the levels of malondialdehyde (MDA) and nitric oxide (NO) in the edema paw. The results showed that TC(MeOH) (1.0 and 2.0 g/kg) and betulin (30 and 90 mg/kg), significantly inhibited the acetic acid-induced writhing response. TC(MeOH) (2.0 g/kg) and betulin (30 and 90 mg/kg) significantly inhibited formalin-induced licking time during both the early and late phases. TC(MeOH) (0.5, 1.0 and 2.0 g/kg) and betulin (30 and 90 mg/kg) also significantly decreased the paw edema at the 4th hour after lambda-carrageenan injection. Furthermore, TC(MeOH) and betulin treatment also significantly increased the activities of SOD, GR and GPx in the liver while decreasing the level of MDA in the edema paw. Finally, betulin (30 and 90 mg/kg) also caused considerable reduction of NO level in the edema paw. Taken together, the present results indicated that TC(MeOH) and betulin possessed analgesic and anti-inflammatory effects. The anti-inflammatory mechanism of TC(MeOH) and betulin may be related to decreasing the levels of MDA and NO in the edema paw by increasing the activities of antioxidant enzymes in the liver.
Lin
Ying-Chih
YC
Department of Optometry, Jen-Teh Junior College of Medicine, Nursing and Management, No. 79-9, Sha-Luen-Hu, Xi Zhou Li, Hou-Loung Town, Miaoli County 35664, Taiwan.
Cheng
Hao-Yuan
HY
Huang
Tai-Hung
TH
Huang
Hsin-Wei
HW
Lee
Yi-Hsuan
YH
Peng
Wen-Huang
WH
D000698:Analgesia / Q000379:methods; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan / Q000009:adverse effects; D004487:Edema / Q000188:drug therapy*; D008099:Liver / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D010146:Pain / Q000188:drug therapy* / Q000378:metabolism; D010147:Pain Measurement / Q000379:methods; D010636:Phenols / Q000378:metabolism; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D028083:Scrophulariaceae; D014315:Triterpenes / Q000494:pharmacology / Q000627:therapeutic use*; D000094062:Betulinic Acid
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D002351:Carrageenan; D008315:Malondialdehyde; D009569:Nitric Oxide; D010636:Phenols; D010936:Plant Extracts; D014315:Triterpenes; C501911:antioxidant biofactor AOB; C002503:betulin; D000094062:Betulinic Acid