Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production.
Hairy roots of Nasturtium officinale, Barbarea verna and Arabis caucasica with active glucosinolate-myrosinase system were obtained after transformation with Agrobacterium rhizogenes. Hairy roots of N. officinale produced phenylalanine-derived gluconasturtiin and glucotropaeolin (max. 24 and 7 mg g(-1) DW). B. verna and A. caucasica hairy roots produced gluconasturtiin (max. 41 mg g(-1) DW) and methionine-derived glucoiberverin (max. 32 mg g(-1) DW), respectively. Treatment of the roots with amino acid precursors of glucosinolate or/and cysteine biosynthesis increased levels of glucosinolate production, combinations of phenylalanine with cysteine (for gluconasturtiin and glucotropaeolin) and methionine with o-acetylserine (for glucoiberverin) were the most effective.
Wielanek
Marzena
M
Department of Plant Physiology and Biochemistry, University of Łódź, 12/16 Banacha, 90-237 Lodz, Poland. mawie@biol.uni.lodz.pl
Królicka
Aleksandra
A
Bergier
Katarzyna
K
Gajewska
Ewa
E
Skłodowska
Maria
M
D000596:Amino Acids / Q000378:metabolism; D031214:Arabis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D031216:Barbarea / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D005961:Glucosinolates / Q000096:biosynthesis* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D017879:Isothiocyanates / Q000378:metabolism; D031223:Nasturtium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D000596:Amino Acids; D005961:Glucosinolates; D017879:Isothiocyanates; C443697:gluconasturtiin; C060321:glucotropeolin; D006026:Glycoside Hydrolases; C022739:thioglucosidase