title: Determination of azole antifungal medicines using zero-order and derivative UV spectrophotometry.
abstract: This paper presents a new methodology of quantitative determination of seven azole antifungal medicines widely used in therapy. Analyses were performed directly by using zero-order (fluconazole), first derivative (bifonazole, clotrimazole, econazole, itraconazole, miconazole) and second derivative (ketoconazole) UV spectrophotometry. Validation of all methods confirms their proper precision (%RSD = 0.47-2.86), recovery (98.7-101.4) and linearity (r coefficient over 0,999) in concentrations under investigation. The parameters received enable the developed procedure to be used in quantitative and as auxiliary in qualitative pharmaceutical analysis.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Collegium Medicum, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland.
authors.forename: Radosław J
authors.initials: RJ
authors.lastname: Ekiert
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
mesh_terms: D000935:Antifungal Agents / Q000032:analysis*; D001393:Azoles / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D001393:Azoles