[Evaluation of the INNO-LiPA MYCOBACTERIA v2 for mycobacterial identification].
OBJECTIVE
Evaluation of the INNO-LiPA MYCOBACTERIA v2 (the INNO-LiPA assay) for mycobacterial identification.


METHODS
The laboratory identifications consisting of Cobas Amplicor systems, AccuProbe, and DDH, are commonly used to identify mycobacterial isolates in Japan. We compared the results between the INNO-LiPA assay and the common methods. A total of 122 clinical isolates from NHO Kinki-chuo Chest Medical Center from 1 February to 30 June 2006 were tested.


RESULTS
There was agreement between the INNO-LiPA assay and the common methods for 112 mycobacterium isolates. The six discordant isolates have showed same results between sequencings and the INNO-LiPA assay. The one M. fortuitum isolates was indicated correctness by DDH and the one M. intracellulare isolates was recognized by Cobas Amplicor systems and as MAC by AccuProbe MAC. Moreover, discrepant results between sequencings and mycobacterial identifications including the INNO-LiPA assay were 2 isolates (M. paraffinicum, M. mucogenicum variant type).


CONCLUSIONS
The INNO-LiPA assay could provide rapid and correct identification results with clear-cut and easy interpretation.
Yoshida
Shiomi
S
Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan. dustin@kch.hosp.go.jp
Suzuki
Katsuhiro
K
Tsuyuguchi
Kazunari
K
Iwamoto
Tomotada
T
Tomita
Motohisa
M
Okada
Masaji
M
Sakatani
Mitsunori
M
D001431:Bacteriological Techniques / Q000379:methods*; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011933:Reagent Kits, Diagnostic
D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic