title: [An infrared imaging system for detecting electrophoretic mobility shift of DNA-protein complexes].
abstract: OBJECTIVE
  To establish a new non-radioactive method for electrophoretic mobility shift assay (EMSA) to investigate the binding between glucocorticoid induced leucine zipper (GILZ) and peroxisome proliferator-activated receptor-gamma 2 (PPARgamma2) promoter oligonucleotides.
  
  
  METHODS
  GILZ protein prepared by prokaryotic expression was linked to PPARgamma2 promoter oligonucleotides end-labeled with IRDye 800 infrared dye. The DNA-protein complex was separated with non-denatured polyacrylamide gel and scanned with the Odyssey. Infrared Imaging System.
  
  
  RESULTS
  One lane of DNA-protein complex was clearly presented, and the signal intensity increased along with the increment of the protein load.
  
  
  CONCLUSIONS
  This infrared imaging system can be used for EMSA for detecting the DNA-protein complex with high sensitivity efficiency and allows easy operation.
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: De-hong
authors.initials: DH
authors.lastname: Cai
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Yong-yan
authors.initials: YY
authors.lastname: Mo
authors.forename: Na
authors.initials: N
authors.lastname: Yi
authors.forename: Fei-yue
authors.initials: FY
authors.lastname: Xing
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D005786:Gene Expression Regulation; D006801:Humans; D007259:Infrared Rays; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000502:physiology; D011506:Proteins / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D011506:Proteins; D004247:DNA