Effect of isoflavone extracts from Glycine max on human endothelial cell damage and on nitric oxide production.
OBJECTIVE
In this study, we determined the protective effect of isoflavones from Glycine max on human umbilical vein endothelial cell (ECV304) damage induced by hydrogen peroxide (H2O2) and on nitric oxide (NO) production.


METHODS
We studied the regulation of NO synthesis in cultured human endothelial cells by phytoestrogens contained in soy extracts in the presence or absence of ICI 182,780 or N(omega)-nitro-L-arginine methyl esther and determined the protective effect of these isoflavones on ECV304 damage induced by H2O2.


RESULTS
We show that soy extracts activate NO synthesis in endothelial cells and protect against cell damage.


CONCLUSIONS
In conclusion, soy isoflavones markedly protect ECV304 cells against H2O2 damage and promote NO synthesizing. Therefore, these isoflavones can potentially act as an NO promoter and as an antioxidant.
Paulo
Michele
M
Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo, Ribeirão Preto, Brazil.
Salvador
Mirian Mendonça
MM
dos Anjos Neto Filho
Mário
M
Montes
Marlise Bonetti Agostinho
MB
Franceschini
Silvio Antônio
SA
Toloi
Maria Regina Torqueti
MR
D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000187:drug effects; D047629:Estrogen Receptor beta / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D018384:Oxidative Stress / Q000187:drug effects*; D013025:Glycine max / Q000737:chemistry; D014471:Umbilical Veins
D000975:Antioxidants; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D007529:Isoflavones; D009569:Nitric Oxide; C004742:daidzein; D006861:Hydrogen Peroxide; D019833:Genistein