[Studies on extraction of active fraction from Rhizoma et Radix Ligustici by supercritical CO2 extraction].
OBJECTIVE
To study the conditions of extraction of active fraction in Rhizoma et Radix Ligustici by supercritical CO2 extraction (SFE).


METHODS
Using the content of ferulic acid as the index, conditions for the extraction including extracting pressure, extracting temperature, temperature of resolution and extracting time were optimized by uniform design.


RESULTS
The best SFE conclusions were as follows: cosolvent was 50% ethanol (ml/g), extracting pressure was 40 MPa, extracting temperature was 50 degrees C, pressure of resolution was 6 MPa, temperature of resolution was 50 degrees C, extracting time was 3.5 hours.


CONCLUSIONS
The optimum extraction has high extraction ratio of active fraction in Rhizoma et Radix Ligustici, so it is reasonable and practicable.
Mao
Cai-Ni
CN
Hainan Medical College, Haikou 571101, China.
He
Wei
W
Li
Yong
Y
Yuan
Wan-Rui
WR
D019661:Apiaceae / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis* / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D009822:Oils, Volatile / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D003373:Coumaric Acids; D009822:Oils, Volatile; D002245:Carbon Dioxide; D000431:Ethanol; C004999:ferulic acid