Identification of a response element in a herpesvirus saimiri mRNA recognized by the ORF57 protein.
The herpesvirus saimiri (HVS) ORF57 protein binds viral RNA, enabling the efficient nuclear export of intronless viral mRNAs. However, it is not known how ORF57 recognizes these viral mRNAs. In this study, a systematic evolution of ligands by exponential enrichment (SELEX) approach was used to select RNA sequences that are preferentially bound by the ORF57 protein. Results identified a recurring motif, GAAGRG, within the majority of selected RNAs, which is also present in many late HVS mRNAs. RNA immunopreciptations demonstrated that disruption of this motif within a viral intronless RNA ablates ORF57 binding. These data suggest that the GAAGRG motif may be required within a HVS intronless mRNA for recognition by the ORF57 protein.
Colgan
Kevin J
KJ
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Boyne
James R
JR
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Whitehouse
Adrian
A
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.|Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
D000818:Animals; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D052156:SELEX Aptamer Technique; D015534:Trans-Activators / Q000378:metabolism*
C415247:ORF57 protein, Herpesvirus saimiri; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators