title: Enantioseparation of amino acid amides on an (S)-N-(3,5-dinitrobenzoyl)leucine-N-phenyl-N-propylamide-bonded silica in normal phase CEC.
abstract: (S)-N-(3,5-Dinitrobenzoyl)leucine-N-phenyl-N-propylamide-bonded silica was used as a chiral stationary phase for separation of enantiomers of some racemic pi-acidic and pi-basic amino acid amides in normal phase capillary LC (CLC) and CEC. For generation of EOF in CEC, different amounts of water were added to n-hexane-isopropanol (IPA) eluents. Influences of added water and composition of polar modifier on retention, enantioselectivity and resolution were studied. Generally better separation was obtained in the eluents of lower water content. Shorter retention and better enantioselectivities were observed in the eluents of higher IPA content, whereas resolution became lower as IPA content was increased. Enantioselectivities were comparable but resolutions were much better in CEC than CLC.
authors.affiliation: Department of Chemistry Education, Daegu University, Gyeongsan, Korea.
authors.forename: In Whan
authors.initials: IW
authors.lastname: Kim
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Hyun
authors.forename: Jurim
authors.initials: J
authors.lastname: Gwon
authors.forename: Juhyeon
authors.initials: J
authors.lastname: Jin
authors.forename: Jung Hag
authors.initials: JH
authors.lastname: Park
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D000577:Amides / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D053801:Capillary Electrochromatography; D004136:Dinitrobenzenes / Q000737:chemistry; D053841:Electroosmosis; D007930:Leucine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
chemical_list: D000577:Amides; D000596:Amino Acids; D004136:Dinitrobenzenes; D014867:Water; D012822:Silicon Dioxide; D007930:Leucine; D019840:2-Propanol