title: A HaemAtlas: characterizing gene expression in differentiated human blood cells.
abstract: Hematopoiesis is a carefully controlled process that is regulated by complex networks of transcription factors that are, in part, controlled by signals resulting from ligand binding to cell-surface receptors. To further understand hematopoiesis, we have compared gene expression profiles of human erythroblasts, megakaryocytes, B cells, cytotoxic and helper T cells, natural killer cells, granulocytes, and monocytes using whole genome microarrays. A bioinformatics analysis of these data was performed focusing on transcription factors, immunoglobulin superfamily members, and lineage-specific transcripts. We observed that the numbers of lineage-specific genes varies by 2 orders of magnitude, ranging from 5 for cytotoxic T cells to 878 for granulocytes. In addition, we have identified novel coexpression patterns for key transcription factors involved in hematopoiesis (eg, GATA3-GFI1 and GATA2-KLF1). This study represents the most comprehensive analysis of gene expression in hematopoietic cells to date and has identified genes that play key roles in lineage commitment and cell function. The data, which are freely accessible, will be invaluable for future studies on hematopoiesis and the role of specific genes and will also aid the understanding of the recent genome-wide association studies.
authors.affiliation: Department of Haematology, University of Cambridge, National Health Service Blood and Transplant, Cambridge, United Kingdom. naw23@cam.ac.uk
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Watkins
authors.forename: Arief
authors.initials: A
authors.lastname: Gusnanto
authors.forename: Bernard
authors.initials: B
authors.lastname: de Bono
authors.forename: Subhajyoti
authors.initials: S
authors.lastname: De
authors.forename: Diego
authors.initials: D
authors.lastname: Miranda-Saavedra
authors.forename: Debbie L
authors.initials: DL
authors.lastname: Hardie
authors.forename: Will G J
authors.initials: WG
authors.lastname: Angenent
authors.forename: Antony P
authors.initials: AP
authors.lastname: Attwood
authors.forename: Peter D
authors.initials: PD
authors.lastname: Ellis
authors.forename: Wendy
authors.initials: W
authors.lastname: Erber
authors.forename: Nicola S
authors.initials: NS
authors.lastname: Foad
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Garner
authors.forename: Clare M
authors.initials: CM
authors.lastname: Isacke
authors.forename: Jennifer
authors.initials: J
authors.lastname: Jolley
authors.forename: Kerstin
authors.initials: K
authors.lastname: Koch
authors.forename: Iain C
authors.initials: IC
authors.lastname: Macaulay
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Morley
authors.forename: Augusto
authors.initials: A
authors.lastname: Rendon
authors.forename: Kate M
authors.initials: KM
authors.lastname: Rice
authors.forename: Niall
authors.initials: N
authors.lastname: Taylor
authors.forename: Daphne C
authors.initials: DC
authors.lastname: Thijssen-Timmer
authors.forename: Marloes R
authors.initials: MR
authors.lastname: Tijssen
authors.forename: C Ellen
authors.initials: CE
authors.lastname: van der Schoot
authors.forename: Lorenz
authors.initials: L
authors.lastname: Wernisch
authors.forename: Thilo
authors.initials: T
authors.lastname: Winzer
authors.forename: Frank
authors.initials: F
authors.lastname: Dudbridge
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
authors.forename: Cordelia F
authors.initials: CF
authors.lastname: Langford
authors.forename: Sarah
authors.initials: S
authors.lastname: Teichmann
authors.forename: Berthold
authors.initials: B
authors.lastname: Göttgens
authors.forename: Willem H
authors.initials: WH
authors.lastname: Ouwehand
mesh_terms: D001271:Atlases as Topic; D001854:Bone Marrow Cells / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage; D002478:Cells, Cultured; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D006410:Hematopoiesis; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors