title: Cellular senescence in usual type uterine leiomyoma.
abstract: OBJECTIVE
  To evaluate the role of senescence in symptomatic patients with multifibroids.
  
  
  METHODS
  A cohort study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Eighty-six fibroids collected from 14 patients who underwent myomectomy or hysterectomy.
  
  
  METHODS
  Senescence-associated beta-galactosidase (SA-beta-Gal) stain in fresh-frozen tissue; reverse-transcription polymerase chain reaction (RT-PCR); MicroRNA in situ hybridization (MISH); immunohistochemistry in formalin-fixed paraffin-embedded tissue.
  
  
  METHODS
  Senescence measured by percentage of SA-beta-Gal-positive cells; levels of let-7 microRNAs measured by RT-PCR and MISH; expression of p16(INK4a), Ki-67, HMGA1, and HMGA2 scaled by immunoreactivity.
  
  
  RESULTS
  About 58% (48 of 82) of tumors showed significant senescent change (SA-beta-Gal positive) in >10% of the tumor volume. The overall trend was a higher level of senescence in small fibroids and older-aged women. Senescent fibroids were additionally shown to have, high levels of let-7 c, d, and f-2 and a low Ki-67 index.
  
  
  CONCLUSIONS
  Senescence is a common cellular change in a large proportion of usual type fibroids. Similarly, senescence may explain the variation in growth rates of these tumors, and may prove to be an important molecular and cellular target in prevention of fibroid growth.
authors.affiliation: Department of Pathology, New York University School of Medicine, New York, New York, USA.
authors.forename: Jordan
authors.initials: J
authors.lastname: Laser
authors.forename: Peng
authors.initials: P
authors.lastname: Lee
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Wei
mesh_terms: D000328:Adult; D016922:Cellular Senescence / Q000502:physiology*; D015331:Cohort Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D007889:Leiomyoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D054928:Validation Studies as Topic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; D001616:beta-Galactosidase