title: Accelerated wound healing in healing-impaired db/db mice by autologous adipose tissue-derived stromal cells combined with atelocollagen matrix.
abstract: Adipose tissue-derived stromal cells (ATSCs) have recently gained widespread attention as a potential alternate source to bone marrow-derived mesenchymal stem cells with a proliferative capacity and a similar ability to undergo multilineage differentiation. In this study, we evaluated the effectiveness of freshly isolated autologous ATSCs-containing atelocollagen matrix with silicon membrane (ACMS) on wound healing of diabetic (db/db) mice. Cultured ATSCs from (db/db) mice secreted significant amounts of growth factors and cytokines, which are suitable for wound repair. Two full thickness round skin defects were made on the backs of healing-impaired db/db mice. Freshly isolated autologous ATSCs-containing ACMS or ACMS alone were applied to the wounds. Twelve mice were treated and then killed at 1 or 2 weeks (n = 6 each). Histologic sections of the wounds were prepared at each time period after treatment. Histologic examination demonstrated significantly advanced granulation tissue formation, capillary formation, and epithelialization in diabetic healing-impaired wounds treated with autologous ATSCs-containing ACMS, compared with mice treated with ACMS alone. These results suggested that transplantation of autologous ATSCs-containing ACMS significantly accelerated wound healing in diabetic healing-impaired db/db mice.
authors.affiliation: Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Nambu
authors.forename: Satoko
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yanagibayashi
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Azuma
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kiyosawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D012825:Silicon; D017768:Skin, Artificial; D017154:Stromal Cells / Q000637:transplantation*; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; C059821:atelocollagen; D003094:Collagen; D012825:Silicon