Chemotaxis and phagocytosis in neutrophils is independent of coronin 1.
The WD repeat protein family member coronin 1 is exclusively expressed in leukocytes, where it colocalizes with the cortical cytoskeleton. Although initially coronin 1 was believed to regulate F-actin dynamics such as leukocyte motility, phagocytosis, and membrane ruffling, recent work showed that in macrophages, T cells, and B cells, coronin 1 is dispensable for these F-actin dependent processes, instead being involved in the regulation of calcium dependent signaling reactions. In this study, we show that in mice lacking coronin 1 neutrophil populations developed normally, and that coronin 1-deficient neutrophils are fully functional with respect to adherence, membrane dynamics, migration, phagocytosis and the oxidative burst. Therefore, these data argue against a role for coronin 1 in the modulation of F-actin and suggest that coronin 1 is dispensable for neutrophil functioning.
Combaluzier
Benoit
B
Biozentrum, University of Basel, Basel, Switzerland.
Pieters
Jean
J
D000199:Actins / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D020013:Calcium Signaling / Q000235:genetics / Q000276:immunology; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis / Q000235:genetics / Q000276:immunology*
D000199:Actins; D008840:Microfilament Proteins; C072061:coronin proteins; D019255:NADPH Oxidases