title: Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS).
abstract: We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods.
authors.affiliation: Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess St., Manchester, UKM1 7DN.
authors.forename: Derren J
authors.initials: DJ
authors.lastname: Heyes
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Quinn
authors.forename: Paul M
authors.initials: PM
authors.lastname: Cullis
authors.forename: Michael
authors.initials: M
authors.lastname: Lee
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Munro
authors.forename: Nigel S
authors.initials: NS
authors.lastname: Scrutton
mesh_terms: D004579:Electron Transport; D007700:Kinetics; D007834:Lasers; D019001:Nitric Oxide Synthase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D011721:Pyrenes; C121436:thiouredopyrenetrisulfonate; D019001:Nitric Oxide Synthase