Increased levels of oxidative DNA damage attributable to cooking-oil fumes exposure among cooks.
Previous investigations have indicated that cooks are exposed to polycyclic aromatic hydrocarbons (PAHs) from cooking-oil fumes. However, Emission of PAH and their carcinogenic potencies from cooking oil fumes sources have not been investigated among cooks. To investigate the urinary excretion of a marker for oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), in different groups of cooks and different exposure groups, and to study the association between 8-OHdG and 1-hydroxypyrene(1-OHP), a biological marker for PAH exposure. Urine samples were collected from different groups of cooks (n = 86) and from unexposed controls (n = 36); all were male with similar age and smoking habits. The health status, occupational history, smoking, and alcohol consumption 24 h prior to sampling was estimated from questionnaires. The urine samples were frozen for later analyses of 8-OHdG and 1-OHP levels by high-performance liquid chromatography. Excretion in urine of 8-OHdG was similar for controls (mean 1.2micromol/mol creatinine, n = 36), and for those who had been in the kitchen with an exhaust-hood operating (mean 1.5micromol/mol creatinine, n = 45). Cooks exposed to cooking-oil fumes without exhaust-hood operation had significantly increased excretion of 8-OHdG (mean 2.3micromol/mol creatinine, n = 18), compared with controls. The urinary levels of ln 1-OHP and ln 8-OHdG were still significantly correlated in a multiple regression analysis. The results indicate that exposure to PAH or possibly other compounds in cooking-oil fumes may cause oxidative DNA damage.
Ke
Yuebin
Y
Department of Genetic Toxicology, Shenzhen Center for Diseases Control and Prevention, Shenzhen, P.R. China. keyke@tom.com
Cheng
Jinquan
J
Zhang
Zhicheng
Z
Zhang
Renli
R
Zhang
Zhunzhen
Z
Shuai
Zhihong
Z
Wu
Tangchun
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects* / Q000652:urine; D016902:Air Pollution, Indoor / Q000009:adverse effects* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003296:Cooking; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004784:Environmental Monitoring; D006304:Health Status; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects*; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D016877:Oxidants; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D011721:Pyrenes / Q000032:analysis; D011795:Surveys and Questionnaires; D055815:Young Adult
D000395:Air Pollutants, Occupational; D016877:Oxidants; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C033146:1-hydroxypyrene