Construction and characterization of a high activity mutant of human keratinocyte growth factor-2.
Keratinocyte growth factor-2 (KGF-2) plays an important role in vertebrate limb development, lung branching morphogenesis, regeneration and reconstruction of the epidermis. Previous studies have used the wild type factor. Here, we have constructed a double-site mutant of human KGF-2, named STEA. STEA possesses higher receptor binding affinity and promotes better proliferation activity on rat tracheal epithelium (RTE) cells than recombinant human KGF-2. These results suggest that the simultaneous mutation of Ser115 to Thr and Glu117 to Ala improves the biological activity of KGF-2.
Wang
Jinfeng
J
Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, 100850 Beijing, China.
Cai
Xin
X
Zou
Minji
M
Xu
Tao
T
Liu
Shen
S
Wang
Yuanyuan
Y
Wang
Jiaxi
J
Xu
Donggang
D
D019943:Amino Acid Substitution; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000494:pharmacology*; D020125:Mutation, Missense; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects
D051526:Fibroblast Growth Factor 10; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins