Diagnostic model of saliva protein finger print analysis of patients with gastric cancer.
OBJECTIVE
To explore the method for early diagnosis of gastric cancer by screening the expression spectrum of saliva protein in gastric cancer patients using mass spectrometry for proteomics.


METHODS
Proportional peptide mass fingerprints were obtained by analysis based on proteomics matrix-assisted laser desorption ionization time-of-flight/mass spectrometry. A diagnosis model was established using weak cation exchange magnetic beads to test saliva specimens from gastric cancer patients and healthy subjects.


RESULTS
Significant differences were observed in the mass to charge ratio (m/z) peaks of four proteins (1472.78 Da, 2936.49 Da, 6556.81 Da and 7081.17 Da) between gastric cancer patients and healthy subjects.


CONCLUSIONS
The finger print mass spectrum of saliva protein in patients with gastric cancer can be established using gastric cancer proteomics. A diagnostic model for distinguishing protein expression mass spectra of gastric cancer from non-gastric-cancer saliva can be established according to the different expression of proteins 1472.78 Da, 2936.49 Da, 6556.81 Da and 7081.17 Da. The method for early diagnosis of gastric cancer is of certain value for screening special biological markers.
Wu
Zheng-Zhi
ZZ
Southern Medical University, Guangzhou 510515, Guangdong Province, China. zhengzhw@yahoo.com.cn
Wang
Ji-Guo
JG
Zhang
Xiao-Li
XL
D055088:Early Detection of Cancer; D006801:Humans; D008970:Molecular Weight; D009088:Mucoproteins / Q000032:analysis; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D012016:Reference Values; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000175:diagnosis*
D009088:Mucoproteins; D012471:Salivary Proteins and Peptides