Gene expression analysis by oligonucleotide microarray in oral leukoplakia.
BACKGROUND
Oral leukoplakias (LP) are the most frequent types of oral pre-cancerous lesions, but there is no accurate assessment of this malignant transformation or even genetic diagnosis of the oral epithelial dysplasia. We need to identify the new genetic diagnosis system of the epithelial dysplasia.


METHODS
Oligonucleotide microarray was used to analyze expression patterns of 29,952 genes in 10 LP patients. We compared the different gene expressions between mild dysplasia cases and severe dysplasia cases.


RESULTS
Ninety-six genes expressed differentially were selected as candidates for up-regulated in severe dysplasia. Subsequently, we further selected 16 genes with highest differentially expression. By hierarchical clustering analysis, the 10 cases were divided mild dysplasia from severe dysplasia.


CONCLUSIONS
The 16 genes are suggested as biomarker gene sets of efficacy and quickly recognized in the development of oral epithelial dysplasia.
Kuribayashi
Yuri
Y
Oral and Maxillofacial Surgery, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. kuriosur@tmd.ac.jp
Morita
Kei-ichi
K
Tomioka
Hirofumi
H
Uekusa
Masaru
M
Ito
Daisuke
D
Omura
Ken
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D016000:Cluster Analysis; D004848:Epithelium / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation