title: Conserved DNA methylation in Gadd45a(-/-) mice.
abstract: Gadd45a (growth arrest and DNA-damage-inducible protein 45 alpha) plays a pivotal role in cellular stress responses and is implicated in DNA repair, cell cycle arrest and apoptosis.(1) Recently, it was proposed that GADD45A is a key regulator of active DNA demethylation by way of its role in DNA repair.(2) Barreto et al. reported that Gadd45a overexpression activated transcription from methylation-silenced reporter plasmids and promoted global DNA demethylation. siRNA-mediated knockdown of Gadd45a levels resulted in increased levels of DNA methylation at specific endogenous loci. Based on these exciting results, Gadd45a(-/-) mice might be predicted to have a hypermethylation phenotype. We report here that neither global nor locus-specific methylation is increased in Gadd45a(-/-) mice.
authors.affiliation: Fels Institute for Cancer Research & Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA. noraengel@temple.edu
authors.forename: Nora
authors.initials: N
authors.lastname: Engel
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Tront
authors.forename: Toyin
authors.initials: T
authors.lastname: Erinle
authors.forename: Nghi
authors.initials: N
authors.lastname: Nguyen
authors.forename: Keith E
authors.initials: KE
authors.lastname: Latham
authors.forename: Carmen
authors.initials: C
authors.lastname: Sapienza
authors.forename: Barbara
authors.initials: B
authors.lastname: Hoffman
authors.forename: Dan A
authors.initials: DA
authors.lastname: Liebermann
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D019175:DNA Methylation / Q000187:drug effects; D004260:DNA Repair; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000172:deficiency*; D034741:RNA, Small Interfering / Q000494:pharmacology; D000097642:GADD45 Proteins
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C499072:Gadd45a protein, mouse; D000097642:GADD45 Proteins