[AuCl(4)] (-) and Fe (3+)/[Fe(CN) (6)] (3-) ions-derivated immunosensing interface for electrochemical immunoassay of carcinoembryonic antigen in human serum.
[AuCl(4)](-) was initially deposited by electrochemical reduction on a glassy carbon electrode (GCE) to form porous nanogold layer, then prussian blue (PB) was electrodeposited onto the as-prepared nanogold layer, and then secondary nanogold particles were fabricated again on the PB surface by electrochemical reduction for the immobilization of anti-CEA antibodies. The presence of double-layer porous gold nanoparticles enhanced the immobilized amount of biomolecules, and improved the sensitivity of the immunoassay. PB, as a good redox probe, was facile to electrochemical analysis and measurement. Under optimal conditions, the developed immunoassay exhibited dynamic range from 3.0 to 80.0 ng/mL with a detection limit of 0.9 ng/mL CEA (S/N = 3). Moreover, the selectivity, reproducibility and stability of the immunosensor were acceptable.
Liu
Kaige
K
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry, Chongqing, People's Republic of China. liukaige08@yahoo.com.cn
Yuan
Ruo
R
Chai
Yaqin
Y
Tang
Dianping
D
An
Haizhen
H
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000097:blood*; D002417:Cattle; D002712:Chlorides / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D005295:Ferrocyanides / Q000737:chemistry*; D005898:Glass; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007477:Ions; D053768:Metal Nanoparticles / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods*
D017607:Aluminum Compounds; D002272:Carcinoembryonic Antigen; D002712:Chlorides; D005295:Ferrocyanides; D007477:Ions; D000077410:Aluminum Chloride; C000170:ferric ferrocyanide