Cadherin-20 expression by motor neurons is regulated by Sonic hedgehog during spinal cord development.
During chicken spinal cord development, cadherin-20 is expressed by motor neurons of the lateral motor column and other cells in the basal and alar plates. To investigate the regulation of cadherin-20 expression, Sonic hedgehog (Shh) signaling was altered in chicken spinal cord and hindbrain by in-vivo electroporation. Our results show that, at an early embryonic stage 12, Shh induces cadherin-20 expression by motor neurons. Later, at stage 24 when the sorting of motor neuron pools begins, cadherin-20 expression is induced in the neural progenitors of the ventricular zone but not in motor neurons. Blockage of Shh signaling inhibits cadherin-20 expression in the motor column. Therefore, cadherin-20 expression in motor neurons is regulated by Shh in a time-dependent manner.
Luo
Jiankai
J
Institute of Anatomy I, Friedrich Schiller University Jena, Germany. jiankai.luo@uni-rostock.de
Ju
Min Jeong
MJ
Lin
Juntang
J
Yan
Xin
X
Markus
Annett
A
Mix
Eilhard
E
Rolfs
Arndt
A
Redies
Christoph
C
D000818:Animals; D017209:Apoptosis; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002642:Chick Embryo; D018274:Electroporation; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D009046:Motor Neurons / Q000378:metabolism*; D012249:Rhombencephalon / Q000196:embryology / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism*
D015820:Cadherins; D053823:Hedgehog Proteins