Vitrification of isolated mice blastomeres using a closed loading device.
Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic information. A lot of developing technologies may benefit by the efficient cryopreservation of blastomeres for future potential use, especially for stem cell culture and differentiation control. We are hereby reporting for the first time the feasibility of preserving individual isolated blastomeres in microvolumes in a closed vitrification system. Using a cryotip and propagation in microvolumes, isolated mice blastomeres were vitrified and warmed with 100% post-warming survival.
Kader
Amr
A
Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics & Gynecology and Women's Health Institute, Cleveland Clinic, Cleveland, Ohio, USA. agarwaa@ccf.org
Agarwal
Ashok
A
Sharma
Rakesh
R
Falcone
Tommaso
T
D000818:Animals; D001757:Blastomeres; D015925:Cryopreservation / Q000379:methods*; D004249:DNA Damage; D020287:In Situ Nick-End Labeling; D051379:Mice