title: [Effect of angiotensin II on pregnancy-associated plasma protein A and insulin-like growth factor 1 gene expression in human umbilical artery smooth muscle cells].
abstract: OBJECTIVE
  To study the effect of angiotensin II (Ang II) on pregnancy-associated plasma protein A (PAPP-A) and insulin-like growth factor 1 (IGF-1) mRNA expressions in human umbilical artery smooth muscle cells (HUVSMCs).
  
  
  METHODS
  In the presence or absence of Ox-LDL, HUVSMCs were cultured with Ang II of 10(-5) mol/L for 0, 6, 12, 24, 36, and 48 h, or with Ang II at the concentrations of 10(-7), 10(-6), 10(-5), and 10(-4) mol/L for 24 h, after which the cells were then collected to detect PAPP-A and IGF-1 mRNA expressions in the cells using RT-PCR.
  
  
  RESULTS
  At the concentration of 10(-5) mol/L, Ang II showed a time-dependent effect in inducing PAPP-A and IGF-1 mRNA expressions, which began to increase at 12 h of culture and reaching the highest level at 24 h. Ang II also dose-dependently induced PAPP-A and IGF-1 mRNA expressions, and 10(-5) mol/L Ang II induced the highest expression levels of the two genes. Ox-LDL exposure significantly further increased the expression levels of PAPP-A and IGF-1 mRNA in the cells regardless of the Ang II concentration or duration for cell treatment (P<0.05).
  
  
  CONCLUSIONS
  Ang II can time- and dose-dependently induces PAPP-A and IGF-1 mRNA expression in HUVSMCs and is responsible for inducing platelet activity and inflammatory reaction in acute coronary syndromes, and the effects of Ang II can be enhanced by Ox-LDL.
authors.affiliation: Postgraduate School, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zha
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Zhang
authors.forename: Zhi-xin
authors.initials: ZX
authors.lastname: Jiang
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Hu
authors.initials: H
authors.lastname: Lin
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Liang
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D002478:Cells, Cultured; D004357:Drug Synergism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D011266:Pregnancy-Associated Plasma Protein-A / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014469:Umbilical Arteries / Q000166:cytology / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; D000804:Angiotensin II; D007334:Insulin-Like Growth Factor I; D011266:Pregnancy-Associated Plasma Protein-A