Dysadherin expression in gastrointestinal stromal tumors (GISTs).
The purpose of this study was to detect the protein and mRNA expression of dysadherin and to investigate the clinical significance of dysadherin expression in gastrointestinal stromal tumors (GISTs). Twenty fresh GISTs samples were used for testing the mRNA of dysadherin and E-cadherin by reverse-transcription polymerase chain reaction (RT-PCR). In addition, 54 paraffin blocks of GISTs cases were also used for the investigation of dysadherin and E-cadherin using immunohistochemistry. The dysadherin expression level was significantly correlated with GISTs risk stratification (chi(2)=5.769, P<0.05), but was not related to age, sex, histologic subtype, and location (P>0.05). There was only faint or absent E-cadherin protein expression in GISTs. The expression level of dysadherin is related to the risk of GISTs. Dysadherin upregulation, which may cause loss of E-cadherin, is one of the reasons for GISTs recurrence and metastasis. It seems that dysadherin protein detection is a promising method for GISTs prognostication.
Liang
Jian Fang
JF
Department of Pathology, The First Affiliated Hospital, Shanxi Medical University, 030001 Taiyuan, China. Jfliang2000@yahoo.com.cn
Zheng
Hui Xia
HX
Xiao
Hong
H
Li
Ning
N
Cheng
Cai Xia
CX
Wang
Hong Kun
HK
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D015820:Cadherins / Q000032:analysis / Q000235:genetics; D005260:Female; D046152:Gastrointestinal Stromal Tumors / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007473:Ion Channels; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D008840:Microfilament Proteins; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D055815:Young Adult
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C451410:FXYD5 protein, human; D007473:Ion Channels; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger