title: Dispersed phantom scatterer technique reveals subtle differences in substrate recognition by phospholipase D inactive mutants.
abstract: An elastic light-scattering-based method that makes use of phantom nanoparticles as a substrate organizer (see illustration) allowed the quantitative evaluation of the molecular recognition events between a series of inactivated mutants of phospholipase D and lysophosphatidylcholine. The results highlight the remarkable effects on binding capability caused by single amino acid substitutions.
authors.affiliation: Istituto Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, Milan, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Morasso
authors.forename: Tommaso
authors.initials: T
authors.lastname: Bellini
authors.forename: Daniela
authors.initials: D
authors.lastname: Monti
authors.forename: Mattia
authors.initials: M
authors.lastname: Bassi
authors.forename: Davide
authors.initials: D
authors.lastname: Prosperi
authors.forename: Sergio
authors.initials: S
authors.lastname: Riva
mesh_terms: D000818:Animals; D002417:Cattle; D008027:Light; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D053758:Nanoparticles / Q000737:chemistry; D010739:Phospholipase D / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D012542:Scattering, Radiation; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D050505:Mutant Proteins; D010739:Phospholipase D