title: Revisiting optical clearing with dimethyl sulfoxide (DMSO).
abstract: Functional optical characterization of disease progression and response to therapy suffers from loss of spatial resolution and imaging depth due to scattering. Here we report on the ability of dimethyl sulfoxide (DMSO) alone to reduce the optical scattering of skin. We observed a threefold reduction in the scattering of skin with topical DMSO application. With an in vivo window chamber model, we observed a threefold increase in light transmittance through the preparation and enhanced visualization of subsurface microvasculature. Collectively, our data demonstrate the potential of DMSO alone to mitigate effects of scattering, which we expect will improve molecular imaging studies.
authors.affiliation: School of Biological Sciences, University of California, Irvine, California 92697, USA.
authors.forename: Albert K
authors.initials: AK
authors.lastname: Bui
authors.forename: R Anthony
authors.initials: RA
authors.lastname: McClure
authors.forename: Jennell
authors.initials: J
authors.lastname: Chang
authors.forename: Charles
authors.initials: C
authors.lastname: Stoianovici
authors.forename: Jason
authors.initials: J
authors.lastname: Hirshburg
authors.forename: Alvin T
authors.initials: AT
authors.lastname: Yeh
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
mesh_terms: D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D012867:Skin / Q000187:drug effects*
chemical_list: D004121:Dimethyl Sulfoxide