Proteomic global profiling for cancer biomarker discovery.
The ultimate goal of cancer molecular diagnostics is the development of simple tests to predict cancer risk, detect cancer early, classify tumors, and monitor response to therapy. Proteomics is well suited for these tasks. However, there are substantial challenges that need to be met to identify the most informative markers using proteomics. Approaches for in-depth quantitative proteomic analysis based on isotopic labeling and protein fractionation are presented in this chapter.
Faca
Vitor
V
Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Wang
Hong
H
Hanash
Samir
S
D020106:Acrylamide / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D020033:Protein Isoforms; D020106:Acrylamide