Formation of supported lipid bilayers on indium tin oxide for dynamically-patterned membrane-functionalized microelectrode arrays.
Supported lipid bilayers (SLBs) are ideal platforms for the study of membrane proteins and function. Assembly of functional SLBs in an array format would lead to a breakthrough in high-throughput screening of membrane-associated processes, e.g., drugs binding to transmembrane proteins. We report the formation of SLBs from the rupture of anionic vesicles in the presence of Ca(2+) ions on ITO-coated surfaces and characterise the assembly and SLB properties. Furthermore, the formation, manipulation and regeneration of SLBs adsorbed on ITO microelectrode array spots using an electric potential switch are demonstrated. This platform enables addressable assembly and the study of electrochemically mediated membrane processes in a microarray format which can be regenerated in situ.
Kumar
Karthik
K
BioInterface Group, Laboratory of Surface Science and Technology, Swiss Federal Institute of Technology (ETH), Zürich, CH-8093 Zurich, Switzerland.
Tang
Clarence S
CS
Rossetti
Fernanda F
FF
Textor
Marcus
M
Keller
Beat
B
Vörös
Janos
J
Reimhult
Erik
E
D002021:Buffers; D004563:Electrochemistry; D005456:Fluorescent Dyes; D006531:HEPES; D008051:Lipid Bilayers / Q000138:chemical synthesis*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008839:Microelectrodes; D038761:Photobleaching; D011791:Quartz; D012996:Solutions; D017971:Tin Compounds / Q000737:chemistry*
D002021:Buffers; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D012996:Solutions; D017971:Tin Compounds; D011791:Quartz; C109984:indium tin oxide; D006531:HEPES