Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells.
The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate- resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.
Kasugai
Chikatoshi
C
Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Japan.
Morikawa
Akiko
A
Naiki
Yoshikazu
Y
Koide
Naoki
N
Komatsu
Takayuki
T
Yoshida
Tomoaki
T
Yokochi
Takashi
T
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D015726:Giant Cells / Q000166:cytology / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D000071681:Tartrate-Resistant Acid Phosphatase
D017077:Culture Media, Conditioned; D007527:Isoenzymes; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D047428:Protein Kinase Inhibitors; D003208:Concanavalin A; D020928:Mitogen-Activated Protein Kinases; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase