title: [Effect of AT-1 alpha receptor gene silencing on nuclear factor-kappaB activity in hepatic Kupffer cells].
abstract: OBJECTIVE
  To investigate the effect of angiotensin II type-1 (AT-1) alpha receptor gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic Kupffer cells.
  
  
  METHODS
  The expression of AT-1 alpha receptors in primary isolated cultured hepatic Kupffer cells was detected by immunohistochemistry. pSilencer/AT-1 alpha receptor siRNA plasmids were transfected into Kupffer cells, which were subsequently exposed to 10(-6) mol/L angiotensin II (Ang II) for 60 min. The changes in the DNA binding activity of NF-kappaB in the cells was assessed using electrophoretic gel mobility shift assay (EMSA).
  
  
  RESULTS
  AT-1 alpha receptor expression was detected in Kupffer cells. NF-kappaB DNA binding activity was markedly increased in Kupffer cells after Ang II stimulation, and obviously inhibited by transfectiom with pSilencer/AT-1 alpha receptor siRNA plasmid.
  
  
  CONCLUSIONS
  Ang II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor.
authors.affiliation: Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. mylx99@163.com
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.forename: Gao-su
authors.initials: GS
authors.lastname: Zhou
authors.forename: Zhen-shu
authors.initials: ZS
authors.lastname: Zhang
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D007728:Kupffer Cells / Q000166:cytology*; D016328:NF-kappa B / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D034741:RNA, Small Interfering; D044140:Receptor, Angiotensin, Type 1