Composition of transgenic Volvariella volvacea tolerant to cold stress is equivalent to that of conventional control.
Transgenic Volvariella volvacea strains, which are tolerant to cold stress, were generated by the stable insertion of antifreeze protein gene isolated from budworm into the genome of a conventional variety V23 of V. volvacea. As a part of the safety assessment program, transgenic V. volvacea strains were compared to a nontransgenic near-isogenic V. volvacea strain V23 grown contemporaneously by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring proximates, amino acids, fatty acids, minerals, vitamins, 5'-nucleotides, nucleic acid, and antinutrients such as tannin and cyanide in V. volvacea strains harvested at egg-shaped stage. Results of the comparisons indicate that these transgenic strains are compositionally equivalent to the conventional control.
Wang
Jie
J
Department of Bioengineering, College of Food Science, and Institute of Biomass Research, South China Agricultural University, Guangzhou, China.
Guo
Liqiong
L
Lin
Junfang
J
D000596:Amino Acids / Q000032:analysis; D021301:Antifreeze Proteins / Q000235:genetics; D003080:Cold Temperature; D005227:Fatty Acids / Q000032:analysis; D008903:Minerals / Q000032:analysis; D009711:Nucleotides / Q000032:analysis; D030781:Organisms, Genetically Modified; D014815:Vitamins / Q000032:analysis; D055367:Volvariella / Q000737:chemistry*
D000596:Amino Acids; D021301:Antifreeze Proteins; D005227:Fatty Acids; D008903:Minerals; D009711:Nucleotides; D014815:Vitamins