title: Class IIC alpha-mannosidase AfAms1 is required for morphogenesis and cellular function in Aspergillus fumigatus.
abstract: The mammalian ER/cytosolic alpha-mannosidase (Man2C1p), yeast vacuolar alpha-mannosidase (Ams1p) and the Aspergillus nidulans alpha-mannosidase are members of Class IIC subgroup, which is involved in oligosaccharide catabolism and N-glycan processing. Unlike their mammalian counterparts, the yeast Ams1p and A. nidulans Class IIC alpha-mannosidase are not essential for morphogenesis and cellular function. In this study, the Afams1, a gene encoding a member of Class IIC alpha-mannosidases, was identified in the opportunistic pathogen Aspergillus fumigatus. Deletion of the Afams1 led to a severe defect in conidial formation, especially at a higher temperature. In addition, abnormalities of polarity and septation were associated with the DeltaAfams1 mutant. Our results showed that the Afams1 gene, in contrast to its homolog in yeast or A. nidulans, was required for morphogenesis and cellular function in A. fumigatus.
authors.affiliation: Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.forename: Wenxia
authors.initials: W
authors.lastname: Fang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Haomiao
authors.initials: H
authors.lastname: Ouyang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Luo
authors.forename: Cheng
authors.initials: C
authors.lastname: Jin
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D017353:Gene Deletion; D009024:Morphogenesis / Q000235:genetics; D043323:alpha-Mannosidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D043323:alpha-Mannosidase