Characterization of a PLLA-collagen I blend nanofiber scaffold with respect to growth and osteogenic differentiation of human mesenchymal stem cells.
We developed a nanofiber scaffold by blending PLLA with collagen I, suitable for bone regeneration. Among several PLLA-COLI ratios tested, cell growth was better enhanced when blends with a ratio of PLLA-COLI 4:1 were used. Here, growth as well as osteoblast differentiation of hMSC was improved when compared to PLLA nanofibers alone. Therefore, blending is a suitable tool to enhance PLLA nanofibers with respect to bone tissue engineering.
Schofer
Markus D
MD
Department of Orthopedics, University of Marburg, D-35043 Marburg, Baldingerstrasse, Germany. schofer@med.uni-marburg.de
Boudriot
Ulrich
U
Leifeld
Irini
I
Sütterlin
Romina I
RI
Rudisile
Markus
M
Wendorff
Joachim H
JH
Greiner
Andreas
A
Paletta
Jürgen R J
JR
Fuchs-Winkelmann
Susanne
S
D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*
D024042:Collagen Type I; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)