Anti-biofilm efficacy of nitric oxide-releasing silica nanoparticles.
The ability of nitric oxide (NO)-releasing silica nanoparticles to kill biofilm-based microbial cells is reported. Biofilms of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans were formed in vitro and exposed to NO-releasing silica nanoparticles. Replicative viability experiments revealed that >or= 99% of cells from each type of biofilm were killed via NO release, with the greatest efficacy (>or= 99.999% killing) against gram-negative P. aeruginosa and E. coli biofilms. Cytotoxicity testing demonstrated that the highest dose of NO-releasing silica nanoparticles inhibited fibroblast proliferation to a lesser extent than clinical concentrations of currently administered antiseptics (e.g., chlorhexidine) with proven wound-healing benefits. This study demonstrates the promise of employing nanoparticles for delivering an antimicrobial agent to microbial biofilms.
Hetrick
Evan M
EM
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Shin
Jae Ho
JH
Paul
Heather S
HS
Schoenfisch
Mark H
MH
D000818:Animals; D018441:Biofilms; D002460:Cell Line; D002470:Cell Survival; D005347:Fibroblasts; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D009569:Nitric Oxide; D012822:Silicon Dioxide