Monocyte chemoattractant protein-1-2518 G/A polymorphism, plasma levels, and premature stable coronary artery disease.
BACKGROUND
We examined the -2518G/A polymorphism of the MCP-1 gene, its plasma levels, and premature stable CAD in a Chinese population.


METHODS
The study comprised 132 patients with premature stable CAD (cases) and 153 controls. Genotypes were determined by ligase detection reaction-polymerase chain reaction sequencing and grouping. Plasma MCP-1 level was detected with enzyme-linked immunosorbent assay.


RESULTS
No differences were found between genotype distribution and allele frequencies of MCP-1 gene -2518 G/A polymorphism (AA:18.1%; AG:51.5%; GG:30.3% in cases; AA:16.3%; AG:52.9%; GG:30.7% in controls; P = 0.918). The G allele prevalence was 0.561 in cases and 0.572 in controls (P = 0.786). No significant difference was found in plasma MCP-1 level between cases and controls [(47.50 +/- 26.65) vs. (41.05 +/- 15.71) pg/ml, P = 0.272)] or among the 3 genotypes [AA, (43.49 +/- 10.50) pg/ml; AG, (46.09 +/- 25.08) pg/ml; GG, (40.03 +/- 18.13) pg/ml; P = 0.381]. Logistic regression analysis confirmed the lack of association between MCP-1-2518 G/A single nucleotide polymorphism and premature stable CAD after adjustment for confounding parameters.


CONCLUSIONS
The MCP-1-2518 G/A single nucleotide polymorphism does not affect plasma levels of MCP-1 or susceptibility to premature stable CAD in a Chinese population.
Zhong
Chen
C
Department of Cardiology, The Affiliated ZhongDa Hospital of Southeast University, Nanjing, People's Republic of China.
Luzhan
Zhang
Z
Genshan
Ma
M
Jiahong
Wang
W
Xiaoli
Zhang
Z
Qi
Qian
Q
D000328:Adult; D017668:Age of Onset; D002340:Carotid Artery Diseases / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
C111835:CCL2 protein, human; D018932:Chemokine CCL2