A myocardium tropic adeno-associated virus (AAV) evolved by DNA shuffling and in vivo selection.
To engineer gene vectors that target striated muscles after systemic delivery, we constructed a random library of adeno-associated virus (AAV) by shuffling the capsid genes of AAV serotypes 1 to 9, and screened for muscle-targeting capsids by direct in vivo panning after tail vein injection in mice. After 2 rounds of in vivo selection, a capsid gene named M41 was retrieved mainly based on its high frequency in the muscle and low frequency in the liver. Structural analyses revealed that the AAVM41 capsid is a recombinant of AAV1, 6, 7, and 8 with a mosaic capsid surface and a conserved capsid interior. AAVM41 was then subjected to a side-by-side comparison to AAV9, the most robust AAV for systemic heart and muscle gene delivery; to AAV6, a parental AAV with strong muscle tropism. After i.v. delivery of reporter genes, AAVM41 was found more efficient than AAV6 in the heart and muscle, and was similar to AAV9 in the heart but weaker in the muscle. In fact, the myocardium showed the highest gene expression among all tissues tested in mice and hamsters after systemic AAVM41 delivery. However, gene transfer in non-muscle tissues, mainly the liver, was dramatically reduced. AAVM41 was further tested in a genetic cardiomyopathy hamster model and achieved efficient long-term delta-sarcoglycan gene expression and rescue of cardiac functions. Thus, direct in vivo panning of capsid libraries is a simple tool for the de-targeting and retargeting of viral vector tissue tropisms facilitated by acquisition of desirable sequences and properties.
Yang
Lin
L
Division of Molecular Pharmaceutics, University of North Carolina Eshelman School of Pharmacy, Chapel Hill, NC 27599, USA.
Jiang
Jiangang
J
Drouin
Lauren M
LM
Agbandje-McKenna
Mavis
M
Chen
Chunlian
C
Qiao
Chunping
C
Pu
Dongqiuye
D
Hu
Xiaoyun
X
Wang
Da-Zhi
DZ
Li
Juan
J
Xiao
Xiao
X
D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D009202:Cardiomyopathies / Q000821:virology; D006224:Cricetinae; D032621:DNA Shuffling; D000229:Dependovirus / Q000235:genetics*; D004195:Disease Models, Animal; D018628:Gene Dosage; D015723:Gene Library; D005822:Genetic Vectors / Q000008:administration & dosage; D016679:Genome, Viral / Q000235:genetics; D006321:Heart / Q000821:virology*; D006333:Heart Failure / Q000821:virology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000821:virology; D009500:Neutralization Tests; D009928:Organ Specificity; D012641:Selection, Genetic; D020539:Sequence Analysis, Protein; D014161:Transduction, Genetic
D036022:Capsid Proteins; D007074:Immunoglobulin G; D008156:Luciferases