Determination of articaine in human plasma by liquid chromatography-mass spectrometry and its application in a preliminary pharmacokinetic study.
A specific liquid chromatography-mass spectrometric (LC-MS) method using an ion trap spectrometer was developed for the quantitation of articaine in human plasma. Articaine and the internal standard (trazodone) were extracted in a single step with diethyl-ether from 0.5 mL of alkalinized plasma. The mobile phase consisted of acetonitrile with 0.1% formic acid (40:60, v/v). It was delivered at a flow rate of 0.3 mL/min. The effluent was monitored by MS in positive-ion mode. Ionisation was performed using an electrospray ion source operating at 200 degrees C. Articaine was identified and quantified in SIM mode at m/z 185. Calibration curves were linear over the concentration range of 78.1-5000 ng/mL with determination coefficients>0.996. This method was fast (total run-time<3 min), accurate (bias<16%), and reproducible (intra-assay and inter-assay precision<14%) with a quantitation limit of 78.1 ng/mL. The good specificity and sensitivity achieved by this method allowed the determination of articaine plasma levels in patients following a submucosal infiltration injection of articaine in the patients undergoing a third molar surgery.
Hoizey
Guillaume
G
Laboratoire de Pharmacologie et Toxicologie, CHU de Reims, Reims, France. ghoizey@chu-reims.fr
Lamiable
Denis
D
Gozalo
Claire
C
Miric
Thomas
T
Thomas
Aurélie
A
Binet
Laurent
L
Oget
Olivier
O
Millart
Hervé
H
Mauprivez
Cédric
C
D000779:Anesthetics, Local / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D018687:Antidepressive Agents, Second-Generation / Q000032:analysis; D002138:Calibration; D002355:Carticaine / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008964:Molar, Third; D010149:Postoperative Pain / Q000188:drug therapy; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D014081:Tooth Extraction; D014196:Trazodone / Q000032:analysis
D000779:Anesthetics, Local; D018687:Antidepressive Agents, Second-Generation; D007202:Indicators and Reagents; D002355:Carticaine; D014196:Trazodone