title: Mechanisms of sleep-dependent consolidation of cortical plasticity.
abstract: Sleep is thought to consolidate changes in synaptic strength, but the underlying mechanisms are unknown. We investigated the cellular events involved in this process during ocular dominance plasticity (ODP)-a canonical form of in vivo cortical plasticity triggered by monocular deprivation (MD) and consolidated by sleep via undetermined, activity-dependent mechanisms. We find that sleep consolidates ODP primarily by strengthening cortical responses to nondeprived eye stimulation. Consolidation is inhibited by reversible, intracortical antagonism of NMDA receptors (NMDARs) or cAMP-dependent protein kinase (PKA) during post-MD sleep. Consolidation is also associated with sleep-dependent increases in the activity of remodeling neurons and in the phosphorylation of proteins required for potentiation of glutamatergic synapses. These findings demonstrate that synaptic strengthening via NMDAR and PKA activity is a key step in sleep-dependent consolidation of ODP.
authors.affiliation: Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: Sara J
authors.initials: SJ
authors.lastname: Aton
authors.forename: Julie
authors.initials: J
authors.lastname: Seibt
authors.forename: Michelle
authors.initials: M
authors.lastname: Dumoulin
authors.forename: Sushil K
authors.initials: SK
authors.lastname: Jha
authors.forename: Nicholas
authors.initials: N
authors.lastname: Steinmetz
authors.forename: Tammi
authors.initials: T
authors.lastname: Coleman
authors.forename: Nirinjini
authors.initials: N
authors.lastname: Naidoo
authors.forename: Marcos G
authors.initials: MG
authors.lastname: Frank
mesh_terms: D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D007858:Learning / Q000187:drug effects / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D010766:Phosphorylation; D010775:Photic Stimulation; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism; D012683:Sensory Deprivation / Q000502:physiology; D012890:Sleep / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: D004791:Enzyme Inhibitors; D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D017868:Cyclic AMP-Dependent Protein Kinases