[Tissue distribution of solid lipid nanoparticles loaded garlic oil in rats].
OBJECTIVE
To investigate the tissue distribution of the diallyl disulfide (DADS) and diallyl trisulfide (DATS) in solid lipid nanoparticles loaded garlic oil (GO-SLN) in rats.


METHODS
The gas chromatography-electron capture detection (GC-ECD) method was established to determined the DADS and DATS simultaneously in the biological samples of rats after administration of 0.5 mL garlic oil injection or GO-SLN (containing about 10 mg garlic oil) via jugular vein cannula. The conditions for gas chromatographic separation were as follows. The oven temperature was set at 110 degrees C and maintained for 15 min. Temperatures at the injection port and detector were 180 degrees C and 300 degrees C, respectively. Ultra-pure nitrogen (purity > 99.999%, Shenyang Kerui Special Gases Co. Ltd., China) was used as a carrier gas and made-up gas at flow-rates of 1 mL x min(-1) and 60 mL x min(-1), respectively. All injections were carried out in the split injection mode with a split ratio of 1:10.


RESULTS
The GC-ECD method was fit for determing the concentration of DADS and DATS in garlic oil. The distribution character of GO-SLN in rats had changed to some extent and the concentration of GO-SLN in tissues was higher than that of GO-Injection.


CONCLUSIONS
The SLN can elevate the passive targeting of drugs and lengthen their action time in tissues.
Sun
Xue-hui
XH
Department of Pharmacy, General Hospital of Shenyang Military Region, Shenyang 110016, China. sunxh4545@163.com
Guo
Tao
T
He
Jin
J
Zhao
Ming-hong
MH
Nie
Shu-fang
SF
D000498:Allyl Compounds / Q000032:analysis / Q000493:pharmacokinetics*; D000818:Animals; D004220:Disulfides / Q000032:analysis / Q000493:pharmacokinetics*; D005260:Female; D005737:Garlic / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000032:analysis / Q000493:pharmacokinetics*
D000498:Allyl Compounds; D004220:Disulfides; D010938:Plant Oils; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide