Hydrogen sulfide destroys lipid hydroperoxides in oxidized LDL.
LOOHs (lipid hydroperoxides) in oxLDL [oxidized LDL (low-density lipoprotein)] are potentially atherogenic compounds. Recently, H2S was identified as the third endogenous gasotransmitter in the vasculature. H2O2 is known to be destroyed by H2S. Assuming that H2S may also react with LOOHs, the results show that H2S can destroy LOOHs in oxLDL. The ability of LOOH-enriched LDL to induce HO-1 (haem oxygenase 1) in endothelial cells was abolished by H2S pretreatment. HPLC analysis showed that 9-HPODE [(9S)-hydroperoxy-(10E,12Z)-octadecadienoic acid], a compound found in oxLDL, was reduced to 9-HODE [(9S)-hydroxy-(10E,12Z)-octadecadienoic acid] in the presence of H2S. Thus H2S may act as an antiatherogenic agent by reducing LOOHs to the less reactive LOHs and could abrogate the pathobiological activity of oxLDL.
Muellner
Markus K
MK
Department of Medical Chemistry, CPP, Medical University Vienna, Austria.
Schreier
Sabine M
SM
Laggner
Hilde
H
Hermann
Marcela
M
Esterbauer
Harald
H
Exner
Markus
M
Gmeiner
Bernhard M K
BM
Kapiotis
Stylianos
S
D000704:Analysis of Variance; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D008041:Linoleic Acids / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; C099252:oxidized low density lipoprotein; C024347:9-hydroxy-10,12-octadecadienoic acid; D008315:Malondialdehyde; C049606:9-hydroperoxy-11,12-octadecadienoic acid; D051547:Heme Oxygenase-1; D006862:Hydrogen Sulfide