Novel application of carbon nanotubes for improving resolution in detecting human serum proteins with native polyacrylamide gel electrophoresis.
This paper describes a novel application of carbon nanotubes for improving the resolution of a native PAGE in the detection of human serum proteins. Carbon nanotubes were functionalized and introduced into the gel of native PAGE system, and the electropherogram showed sharp, clear bands. Furthermore, the separation of some most important proteins was improved, and the established method could be applied for the detection of sera from patients with liver diseases.
Guo
Yandong
Y
College of Chemistry, Beijing Normal University, Institute of Cell Biology, Beijing Normal University, Beijing, People's Republic of China.
Huang
Lingyun
L
Baeyens
Willy R G
WR
Delanghe
Joris R
JR
He
Dacheng
D
Ouyang
Jin
J
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008107:Liver Diseases / Q000097:blood; D037742:Nanotubes, Carbon
D001798:Blood Proteins; D037742:Nanotubes, Carbon