[Research for dependablity of administration of platycodi radix in Tianwang Buxinwan decoction with change of brain inhibitive neurotransmitte in rats by microdialysis].
OBJECTIVE
To study the effects of Tianwang Buxinwan decoction on the contents of amino acids neurotransmitters in corpus striatum of rats to implicate the mechanism of Tianwang Buxinwan promoting and Improving sleeping.


METHODS
Contents of two amino acids neurotransmitters in corpus striatum of rats were prepared by microdialysis technology and were determined by HPLC which involved pre-column derivation with orthophthaladehyde, recersed-phase gradient elution and fluorescence detection.


RESULTS
In the experimental separation condition, Tianwang Buxinwan seemed do not influence three kinds of contents of amino acids neurotransmiters (glutamic acid, glycin, aspartic acid), but TBW seemed increase the content of gamma-GABA in corpus striatum of rats.


CONCLUSIONS
The effects of Tianwang Buxinwan to relieve uneasiness may relate with the inhibitory amino acids gamma-GABA. Tianwang Buxinwan may promote increasing the content of gamma-GABA. This discovery may be helpful for the deep study of related mechanism of Tianwang Buxinwan.
Liu
Ping
P
Traditional Chinese Medicine Pharmacy of PLA General Hospital, Beijing 100853, China. liuping0707@yahoo.com.cn
He
Xin-rong
XR
Zhou
Wen-bin
WB
Shen
Ran-ran
RR
Feng
Fan
F
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D001923:Brain Chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D008297:Male; D017551:Microdialysis; D018377:Neurotransmitter Agents / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D018377:Neurotransmitter Agents; C532371:tianwang buxinwan