title: Region required for protein expression from the stop-start pentanucleotide in the M gene of influenza B virus.
abstract: Segment 7 of influenza B virus encodes two proteins, M1 and BM2. BM2 is expressed from a stop-start pentanucleotide, in which the BM2 initiation codon overlaps with the M1 stop codon. Here, we demonstrate that 45 nucleotides of the 3' end of the M1 coding region, but not the 5' end of the BM2 coding region, are sufficient for the efficient expression of the downstream protein. Placing these 45 nucleotides and the stop-start pentanucleotide in between the coding sequences induced the expression of at least three noninfluenza proteins, suggesting the utility of this system for expressing multiple proteins from one mRNA.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin 53706, USA.
authors.forename: Masato
authors.initials: M
authors.lastname: Hatta
authors.forename: Candice K
authors.initials: CK
authors.lastname: Kohlmeier
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Hatta
authors.forename: Makoto
authors.initials: M
authors.lastname: Ozawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D009981:Influenza B virus / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009711:Nucleotides / Q000235:genetics / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D009711:Nucleotides; D014763:Viral Matrix Proteins