Surface expression of Helicobacter pylori urease subunit B gene E fragment on Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A.
Fragment E of ureB (ureBE) was cloned from a clinical isolate of Helicobacter pylori. A prokaryotic expression vector, pAMJ399, with the ureB fragment E and the Staphylococcus aureus protein A anchor fragment (spaX), was constructed. The fusion protein was expressed under the control of the P170 promoter in Lactococcus lactis. Western blot assay of lactococcal cell wall extracts with a polyclonal chicken antiserum confirmed the immunity of the expressed recombinant protein which was located on the cell surface. These results provide the first report of a surface display system in lactic acid bacteria for the delivery of oral vaccines against Helicobacter pylori.
Song
Dafeng
D
Department of Biotechnology, Zhejiang Gongshang University, 310035, Hangzhou, China.
Gu
Qing
Q
D001426:Bacterial Proteins / Q000235:genetics*; D015153:Blotting, Western; D002473:Cell Wall / Q000201:enzymology; D005822:Genetic Vectors; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics*; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics*; D014510:Urease / Q000235:genetics*
D001426:Bacterial Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D014510:Urease