title: A colonic mineralocorticoid receptor cell model expressing epithelial Na+ channels.
abstract: In the distal colon, the epithelial sodium channel (ENaC) is rate limiting for sodium absorption. Progress in the molecular characterization of ENaC expression and trafficking in response to the mineralocorticoid aldosterone has been hampered, since no epithelial colonic cell line existed expressing functional ENaC stimulated by nanomolar aldosterone via mineralocorticoid receptor (MR). Here, we present a human colonic epithelial cell line inducibly expressing the MR (HT-29/B6-Tet-On-MR) which exhibits aldosterone-dependent ENaC-mediated sodium transport in the presence of the short-chain fatty acid butyrate. Butyrate was necessary for high-level expression of MR which allowed for aldosterone-dependent upregulation of beta- and gamma-ENaC expression. As butyrate alone was not capable of promoting ENaC-mediated sodium transport, aldosterone-induced GILZ (glucocorticoid-induced leucine zipper protein) was identified as a candidate factor increasing apical ENaC levels.
authors.affiliation: Department of Gastroenterology, Infectious Diseases and Rheumatology, Charité Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Bergann
authors.forename: Svenja
authors.initials: S
authors.lastname: Plöger
authors.forename: Anja
authors.initials: A
authors.lastname: Fromm
authors.forename: Sebastian
authors.initials: S
authors.lastname: Zeissig
authors.forename: Steffen A
authors.initials: SA
authors.lastname: Borden
authors.forename: Michael
authors.initials: M
authors.lastname: Fromm
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Schulzke
mesh_terms: D002460:Cell Line; D003106:Colon / Q000378:metabolism*; D053503:Epithelial Sodium Channels / Q000096:biosynthesis*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008954:Models, Biological; D018161:Receptors, Mineralocorticoid / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
chemical_list: D053503:Epithelial Sodium Channels; D018161:Receptors, Mineralocorticoid; D012964:Sodium