title: Bovine papillomavirus type 1 DNA and E5 oncoprotein expression in water buffalo fibropapillomas.
abstract: Papillomas and fibropapillomas may occur in the skin and in different organs in animals. Ten different genotypes of bovine papillomavirus (BPV) have been identified. BPV-1 through BPV-10 are all strictly species-specific, but BPV-1/2 may also infect other species such as equids, inducing fibroblastic tumors. BPV-1 and BPV-2 are associated with fibropapillomas in cattle; these tumors are formed by excessive proliferation of virus-infected dermal fibroblasts and epidermal keratinocytes. Nine water buffalo (Bubalus bubalis) were examined for the presence of multiple cutaneous and perivulvar tumors. Cutaneous and perivulvar fibropapillomatosis were confirmed histologically. Negative-stain transmission electron microscopic examination revealed papillomavirus-like particles in the fibropapillomas, and papillomaviral DNA was also detected by the polymerase chain reaction. The amplified long control region (LCR) DNA sequence was identical to that of BPV-1. The BPV-1 E5 oncoprotein was strongly expressed in the tumor cells thus confirming a causal role of the virus. This article represents the first report of cutaneous, perivulvar, and vulvar fibropapilloma associated with BPV-1 infection in the water buffalo and describes another example of cross-species infection by BPV-1.
authors.affiliation: Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Silvestre
authors.forename: G
authors.initials: G
authors.lastname: Borzacchiello
authors.forename: D
authors.initials: D
authors.lastname: Nava
authors.forename: G
authors.initials: G
authors.lastname: Iovane
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: D
authors.initials: D
authors.lastname: Vecchio
authors.forename: F
authors.initials: F
authors.lastname: D'Ausilio
authors.forename: E A
authors.initials: EA
authors.lastname: Gault
authors.forename: M S
authors.initials: MS
authors.lastname: Campo
authors.forename: O
authors.initials: O
authors.lastname: Paciello
mesh_terms: D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism*; D002020:Buffaloes / Q000821:virology*; D004247:DNA / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D019901:Locus Control Region / Q000235:genetics; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010212:Papilloma / Q000378:metabolism* / Q000648:ultrastructure; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D009856:Oncogene Proteins, Viral; C054304:oncogene protein E5, Bovine papillomavirus type 1; D004247:DNA