Selectivity and cooperativity of modulatory ions in a neurotransmitter receptor.
Ions play a modulatory role in many proteins. Kainate receptors, members of the ionotropic glutamate receptor family, require both monovalent anions and cations in the extracellular milieu for normal channel activity. Molecular dynamics simulations and extensive relative binding free energy calculations using thermodynamic integration were performed to elucidate the rank order of binding of monovalent cations, using x-ray crystal structures of the GluR5 kainate receptor dimers with bound cations from the alkali metal family. The simulations show good agreement with experiments and reveal that the underlying backbone structure of the binding site is one of the most rigid regions of the protein. A simplified model where the partial charge of coordinating oxygens was varied suggests that selectivity arises from the presence of two carboxylate groups. Furthermore, using a potential of mean force derived from umbrella sampling, we show that the presence of cations lower the energy barrier for anion approach and binding in the buried anion binding cavity.
Vijayan
Ranjit
R
Department of Biochemistry, University of Oxford, United Kingdom.
Plested
Andrew J R
AJ
Mayer
Mark L
ML
Biggin
Philip C
PC
D001665:Binding Sites; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D007477:Ions / Q000737:chemistry*; D008672:Metals, Alkali / Q000737:chemistry; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D011487:Protein Conformation; D055503:Protein Multimerization; D018092:Kainic Acid Receptors / Q000737:chemistry*; D013816:Thermodynamics
D007477:Ions; D008672:Metals, Alkali; D010100:Oxygen; D018092:Kainic Acid Receptors