title: Determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow-injection analysis based on a specific condensation reaction between malonic acid and ethylenediamine.
abstract: A sensitive and rapid flow-injection analysis was developed for the determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl), which was used for the formation of amide (peptide) and esters as a dehydration or condensation reagent. The EDC.HCl could be determined by the flow-injection analysis based on a specific condensation reaction between malonic acid and ethylenediamine in aquatic media. The reaction was accelerated at 60 degrees C, and the absorbance of the product was detected at 262 nm. The calibration graph of EDC.HCl showed good linearity in the range from 0 to 0.1% (0 to 0.0005 M), whose regression equation was y = 1.52 x 10(9)x (y, peak area; x, % concentration of EDC.HCl). The proposed method allowed high-throughput analysis; the sample throughput was 12 samples per hour. The limit of detection (LOD) and the relative standard deviation (RSD) were 2 x 10(-6) M and 1.0%, respectively. This reaction is proceeded in aqueous solution and specific for EDC.HCl.
authors.affiliation: Chemistry and Biochemistry, Graduate School of Natural Science and Technology & Department of Chemistry, Faculty of Science, Okayama University, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Seno
authors.forename: Kazuki
authors.initials: K
authors.lastname: Matumura
authors.forename: Koji
authors.initials: K
authors.lastname: Oshita
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Oshima
authors.forename: Shoji
authors.initials: S
authors.lastname: Motomizu
mesh_terms: D005022:Ethyldimethylaminopropyl Carbodiimide / Q000032:analysis*; D005029:Ethylenediamines / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D008314:Malonates / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D005029:Ethylenediamines; D008314:Malonates; C031234:ethylenediamine; C030290:malonic acid; D005022:Ethyldimethylaminopropyl Carbodiimide