title: Exploiting ESTs in human health.
abstract: Expressed Sequence Tags (ESTs) are fragments of cDNA clones. They correspond to the most abundant type of cDNA information available in the public databases. ESTs have been used for expression profiling, gene identification, characterization of differentially expressed genes, and identification of transcript variants among other utilities. In this review I will discuss the major features of the collection of ESTs available in the public domain giving a special emphasis on how this dataset has been used in studies about human diseases.
authors.affiliation: Ludwig Institute for Cancer Research, São Paulo, Brazil.
authors.forename: Sandro José
authors.initials: SJ
authors.lastname: de Souza
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D003196:Computer Graphics; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000639:trends; D014158:Transcription, Genetic; D014584:User-Computer Interface
chemical_list: D012333:RNA, Messenger