Preliminary genome-wide association study of bipolar disorder in the Japanese population.
Recent progress in genotyping technology and the development of public databases has enabled large-scale genome-wide association tests with diseases. We performed a two-stage genome-wide association study (GWAS) of bipolar disorder (BD) in Japanese cohorts. First we used Affymetrix 100K GeneChip arrays in the analysis of 107 cases with bipolar I disorder and 107 controls, and selected markers that were nominally significant (P < 0.01) in at least one of the three models (1,577 markers in total). In the follow-up stage, we analyzed these markers using an Illumina platform (1,526 markers; 51 markers were not designable for the platform) and an independent sample set, which consisted of 395 cases (bipolar I + II) and 409 controls. We also assessed the population stratification of current samples using principal components analysis. After the two-stage analysis, 89 markers remained nominally significant (allelic P < 0.05) with the same allele being consistently over-represented in both the first and the follow-up stages. However, none of these were significant after correction for multiple-testing by false discovery rates. Sample stratification was virtually negligible. Collectively, this is the first GWAS of BD in the Japanese population. But given the small sample size and the limited genomic coverage, these results should be taken as preliminary.
Hattori
Eiji
E
RIKEN Brain Science Institute, Saitama, Japan.
Toyota
Tomoko
T
Ishitsuka
Yuichi
Y
Iwayama
Yoshimi
Y
Yamada
Kazuo
K
Ujike
Hiroshi
H
Morita
Yukitaka
Y
Kodama
Masafumi
M
Nakata
Kenji
K
Minabe
Yoshio
Y
Nakamura
Kazuhiko
K
Iwata
Yasuhide
Y
Takei
Nori
N
Mori
Norio
N
Naitoh
Hiroshi
H
Yamanouchi
Yoshio
Y
Iwata
Nakao
N
Ozaki
Norio
N
Kato
Tadafumi
T
Nishikawa
Toru
T
Kashiwa
Atsushi
A
Suzuki
Mika
M
Shioe
Kunihiko
K
Shinohara
Manabu
M
Hirano
Masami
M
Nanko
Shinichiro
S
Akahane
Akihisa
A
Ueno
Mikako
M
Kaneko
Naoshi
N
Watanabe
Yuichiro
Y
Someya
Toshiyuki
T
Hashimoto
Kenji
K
Iyo
Masaomi
M
Itokawa
Masanari
M
Arai
Makoto
M
Nankai
Masahiro
M
Inada
Toshiya
T
Yoshida
Sumiko
S
Kunugi
Hiroshi
H
Nakamura
Michiko
M
Iijima
Yoshimi
Y
Okazaki
Yuji
Y
Higuchi
Teruhiko
T
Yoshikawa
Takeo
T
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D001714:Bipolar Disorder / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005819:Genetic Markers; D055106:Genome-Wide Association Study / Q000379:methods*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects; D025341:Principal Component Analysis
D005819:Genetic Markers