title: Platelet proteome changes associated with diabetes and during platelet storage for transfusion.
abstract: Human platelets play a key role in hemostasis and thrombosis and have recently emerged as key regulators of inflammation. Platelets stored for transfusion produce pro-thrombotic and pro-inflammatory mediators implicated in adverse transfusion reactions. Correspondingly, these mediators are central players in pathological conditions including cardiovascular disease, the major cause of death in diabetics. In view of this, a mass spectrometry based proteomics study was performed on platelets collected from healthy and type-2 diabetics stored for transfusion. Strikingly, our innovative and sensitive proteomic approach identified 122 proteins that were either up- or down-regulated in type-2 diabetics relative to nondiabetic controls and 117 proteins whose abundances changed during a 5-day storage period. Notably, our studies are the first to characterize the proteome of platelets from diabetics before and after storage for transfusion. These identified differences allow us to formulate new hypotheses and experimentation to improve clinical outcomes by targeting "high risk platelets" that render platelet transfusion less effective or even unsafe.
authors.affiliation: Fundamental Science Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland Washington 99352, USA. david.springer@pnl.gov
authors.forename: David L
authors.initials: DL
authors.lastname: Springer
authors.forename: John H
authors.initials: JH
authors.lastname: Miller
authors.forename: Sherry L
authors.initials: SL
authors.lastname: Spinelli
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Pasa-Tolic
authors.forename: Samuel O
authors.initials: SO
authors.lastname: Purvine
authors.forename: Donald S
authors.initials: DS
authors.lastname: Daly
authors.forename: Richard C
authors.initials: RC
authors.lastname: Zangar
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Jin
authors.forename: Neil
authors.initials: N
authors.lastname: Blumberg
authors.forename: Charles W
authors.initials: CW
authors.lastname: Francis
authors.forename: Mark B
authors.initials: MB
authors.lastname: Taubman
authors.forename: Ann E
authors.initials: AE
authors.lastname: Casey
authors.forename: Steven D
authors.initials: SD
authors.lastname: Wittlin
authors.forename: Richard P
authors.initials: RP
authors.lastname: Phipps
mesh_terms: D000328:Adult; D000368:Aged; D001771:Blood Banks; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D038982:Integrin alpha2beta1 / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D017713:Platelet Transfusion; D020543:Proteome / Q000032:analysis* / Q000145:classification; D040901:Proteomics / Q000379:methods*; D013997:Time Factors; D055815:Young Adult
chemical_list: D038982:Integrin alpha2beta1; D020543:Proteome