Induction of an oxalate decarboxylase in the filamentous fungus Trametes versicolor by addition of inorganic acids.
In order to improve yields and to reduce the cost of oxalate decarboxylase (OxDC, EC 4.1.1.2), the induction of OxDC in the white-rot fungus Trametes versicolor was studied in this work. OxDC was induced by addition of inorganic acids including hydrochloric acid, sulfuric acid, and phosphoric acid to culture media. The results showed that all the acids could enhance OxDC expression. The activity of the acid-induced OxDC rose continuously. All of the OxDC volumetric activities induced by the inorganic acids were higher than 20.0 U/L and were two times higher than that obtained with oxalic acid. OxDC productivity was around 4.0 U*L(-1)*day(-1). The highest specific activity against total protein was 3.2 U/mg protein at day 8 after induction of sulfuric acid, and the specific activity against mycelial dry weight was 10.6 U/g at day 9 after induction of hydrochloric acid. The growth of mycelia was inhibited slightly when the pH values in culture media was around 2.5-3.0, while the growth was inhibited heavily when the pH was lower than 2.5.
Zhu
Cui Xia
CX
Donghua University, Shanghai, China.
Hong
Feng
F
D000143:Acids / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D025282:Mycelium / Q000254:growth & development; D055454:Trametes / Q000201:enzymology* / Q000254:growth & development
D000143:Acids; D005656:Fungal Proteins; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase