title: The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation.
abstract: Signal transducer and activator of transcription 3 (STAT3), which is activated by cytokines and growth factors, mediates biological actions in many physiological processes. In a previous study, we found that Y14, a core component of the exon-junction complex (EJC) bound to STAT3 and upregulated the transcriptional activity of STAT3 by influencing its DNA-binding activity. In the present study, we demonstrate that STAT3 endogenously interacts with Y14. In addition, we found that MAGOH, a Y14 partner in the EJC, inhibits the STAT3-Y14 complex formation. Furthermore, small-interfering RNA-mediated reduction of MAGOH expression enhanced interleukin-6-induced gene expression. These results indicate that MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and Y14.
authors.affiliation: Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo 060-0812, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Muromoto
authors.forename: Naohisa
authors.initials: N
authors.lastname: Taira
authors.forename: Osamu
authors.initials: O
authors.lastname: Ikeda
authors.forename: Kaname
authors.initials: K
authors.lastname: Shiga
authors.forename: Shinya
authors.initials: S
authors.lastname: Kamitani
authors.forename: Sumihito
authors.initials: S
authors.lastname: Togi
authors.forename: Shiho
authors.initials: S
authors.lastname: Kawakami
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sekine
authors.forename: Asuka
authors.initials: A
authors.lastname: Nanbo
authors.forename: Kenji
authors.initials: K
authors.lastname: Oritani
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D005091:Exons; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C491494:MAGOH protein, human; D009687:Nuclear Proteins; C406243:RBM8A protein, human; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human