Selective isolation of Bacillus thuringiensis from soil by use of L-serine as minimal medium supplement.
The influence of L-serine on the growth of different strains of the genus Bacillus was investigated. It has been observed that the addition of L-serine to minimal synthetic media results in an inhibition in the growth of certain strains of Bacillus spp. but not B. thuringiensis. Then L-serine-resistance phenomenon was used in isolation of B. thuringiensis strains from soil. An isolation method with media supplied with L-serine was compared to the previously applied procedure (isolation on nutrient agar). L-serine-selective medium appeared to be more effective in isolation of Bt strains.
Andrzejczak
Sylwia
S
Department of Microorganisms Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland. sylwia@microb.uni.wroc.pl
Lonc
Elzbieta
E
D001413:Bacillus thuringiensis / Q000254:growth & development / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media; D012694:Serine; D012988:Soil Microbiology
D003470:Culture Media; D012694:Serine