[The expression of B-cell translocation gene 2 in diethylnitrosamine-induced primary hepatocellular carcinoma rat model.].
OBJECTIVE
To investigate the expression and role of B-cell translocation gene 2(BTG2) in the carcinogenesis of hepatocellular carcinoma (HCC).


METHODS
Modified Diethylnitrosamine (DEN)-induced primary hepatocellular carcinoma rat model was established. The expression of BTG2, p53 and cyclinD1 was detected by RT-PCR, western blot and immunohistochemistry.


RESULTS
The BTG2 protein was predominantly localized in the nucleus, with faint cytoplasmic staining in normal liver cells; however, it is mainly a cytoplasmic protein in HCC cells. BTG2 was over-expressed during the early stage after DEN treatment, the expression level peaked at 5 weeks and then it gradually decreased to the normal level after 16 weeks. The expression of cyclin D1 and cyclin E was increased gradually after DEN treatment, and peaked at 16 weeks and 5 weeks respectively. A significant increase in p53 was not observed until 5 weeks after DEN treatment, and it gradually decreased after 16 weeks.


CONCLUSIONS
Decreased expression of BTG2 may be an important step in carcinogenesis of HCC. BTG2 may positively regulate p53 expression and negatively regulate cyclin D1 expression in the carcinogenesis of HCC.
Zhang
Zhi-Min
ZM
Cancer Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Wang
Ge
G
Yang
Zhi-Xiang
ZX
Shan
Jin-Lu
JL
Chen
Chuan
C
Jin
Feng
F
Xu
Wen
W
Li
Qiong
Q
Luo
Xi-Zhong
XZ
Wang
Dong
D
Li
Zeng-Peng
ZP
D000818:Animals; D001402:B-Lymphocytes; D006528:Carcinoma, Hepatocellular; D004052:Diethylnitrosamine; D022781:Hepatocytes / Q000378:metabolism; D008113:Liver Neoplasms; D051381:Rats
D004052:Diethylnitrosamine