title: [Effects of experimental liver injury on the intestinal barrier in rats.].
abstract: OBJECTIVE
  To study the effects of experimental liver injury on the intestinal barrier, and to evaluate the significance of plasma D(-)-lactate, diamine oxidase (DAO) and endotoxin in live injury.
  
  
  METHODS
  Fifty-five rats were randomized into the acute liver failure group (group C, n = 25), acute liver injury group (group B, n = 15), and control group (group A, n = 15). The concentrations of D(-)-lactate, DAO and endotoxin in plasma were detected by spectrophotograph. The morphology and subcellular structure were observed under optical microscope and transmission electron microscope.
  
  
  RESULTS
  Acute liver failure and acute liver injury models were established successfully. The concentrations of D(-)-lactate and DAO in the plasma of experimental groups (group B and C) were significantly higher than those in the control group (P less than 0.05); the concentration of intestinal DAO in experimental groups were significantly lower than that in the control group (P less than 0.05); the level of endotoxin in C group was significantly higher than that in group A and group B (P less than 0.05).
  
  
  CONCLUSIONS
  Liver injury induces hyperpermeability of the rat intestinal mucosal barrier, plasma D(-)-lactate and DAO are sensitive markers for early diagnosis of liver injury, plasma endotoxin may accelerate deterioration of liver function.
authors.affiliation: Department of Center for Liver Disease, Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yao
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Xu
authors.forename: Xue-Ping
authors.initials: XP
authors.lastname: Chen
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Shen
authors.forename: Jian-Peng
authors.initials: JP
authors.lastname: Gao
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: You
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Yang
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D007413:Intestinal Mucosa; D007422:Intestines; D008111:Liver Function Tests; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006631:Amine Oxidase (Copper-Containing)