title: Kinetics of growth and 3-methyl-1-butanol production by meat-borne, coagulase-negative staphylococci in view of sausage fermentation.
abstract: Five species of meat-borne, coagulase-negative staphylococci were screened for their in vitro production of 3-methyl-1-butanol. The highest production level was encountered for Staphylococcus sciuri alphaSG2, despite its poor growth. With respect to Staphylococcus species that are generally applied in sausage starter cultures, production of 3-methyl-1-butanol was higher with Staphylococcus xylosus 3PA6 than with Staphylococcus carnosus 833. Mathematical modelling was used to link the kinetics of 3-methyl-1-butanol production by S. xylosus 3PA6 and S. carnosus 833 in meat simulation medium to bacterial growth and environmental factors, in casu temperature and pH. The specific production rate of 3-methyl-1-butanol was about ten times higher for S. xylosus 3PA6 than for S. carnosus 833, indicating a higher production rate per amount of biomass. This explains the higher concentrations of 3-methyl-1-butanol in the medium with S. xylosus 3PA6, despite its poorer growth.
authors.affiliation: Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Department of Applied Biological Sciences and Engineering, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Ravyts
authors.forename: G
authors.initials: G
authors.lastname: Vrancken
authors.forename: K
authors.initials: K
authors.lastname: D'Hondt
authors.forename: C
authors.initials: C
authors.lastname: Vasilopoulos
authors.forename: L
authors.initials: L
authors.lastname: De Vuyst
authors.forename: F
authors.initials: F
authors.lastname: Leroy
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D005285:Fermentation; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D008461:Meat Products / Q000032:analysis / Q000382:microbiology*; D008954:Models, Biological; D009812:Odorants / Q000032:analysis; D000439:Pentanols / Q000378:metabolism*; D013210:Staphylococcus / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D013552:Swine; D013649:Taste; D013696:Temperature
chemical_list: D000439:Pentanols; C029683:isopentyl alcohol