Effects of chronic ingestion of catechin-rich green tea on hepatic gene expression of gluconeogenic enzymes in rats.
Normal rats were given catechin-rich green tea as drinking fluid and the effects on hepatic gene expression were examined. The results of DNA microarray analysis and quantitative real-time reverse transcription-polymerase chain reaction indicated the down-regulated expression of genes for glucose-6-phosphatase (G6Pase) and fatty acid synthase, and the up-regulated expression of peroxisome proliferator activated receptor alpha in the rats given green tea for 4 weeks as compared with the water-given animals. One may expect anti-diabetic activity by catechin-rich green tea through its chronic down-regulatory effect on G6Pase expression.
Abe
Koichi
K
Graduate School of Nutritional and Environmental Sciences, and Global COE, University of Shizuoka, Shizuoka, Japan.
Okada
Norihisa
N
Tanabe
Hiroki
H
Fukutomi
Ryuuta
R
Yasui
Kensuke
K
Isemura
Mamoru
M
Kinae
Naohide
N
D000818:Animals; D002392:Catechin / Q000494:pharmacology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005786:Gene Expression Regulation; D005943:Gluconeogenesis; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D047493:PPAR alpha / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013662:Tea / Q000378:metabolism*; D013997:Time Factors
D047493:PPAR alpha; D013662:Tea; D002392:Catechin; D064429:Fatty Acid Synthases; D005952:Glucose-6-Phosphatase