A role for autophagolysosomes in dengue virus 3 production in HepG2 cells.
We have recently proposed that amphisomes act as a site for translation and replication of dengue virus (DENV)-2 and that DENV-2 entry and replication are linked through an ongoing association with membranes of an endosomal-autophagosomal lineage. In this report, we present the results of an investigation into the interaction between DENV-3 and the autophagy machinery. Critically, treatment with the lysosomal fusion inhibitor l-asparagine differentiated the interaction of DENV-3 from that of DENV-2. Inhibition of fusion of autophagosomes and amphisomes with lysosomes resulted in decreased DENV-3 production, implying a role for the autophagolysosome in the DENV-3 life cycle. Evidence based upon the co-localization of LC3 and cathepsin D with double stranded RNA and NS1 protein, as assessed by confocal microscopy, support a model in which DENV-3 interacts with both amphisomes and autophagolysosomes. These results demonstrate that the interactions between DENV and the host cell autophagy machinery are complex and may be determined in part by virus-encoded factors.
Khakpoor
Atefeh
A
Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Thailand.
Panyasrivanit
Mingkwan
M
Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Thailand.
Wikan
Nitwara
N
Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Thailand.
Smith
Duncan R
DR
Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Thailand.
D001343:Autophagy / Q000502:physiology*; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D003716:Dengue Virus / Q000145:classification / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology / Q000821:virology*; D006801:Humans; D010588:Phagosomes / Q000502:physiology*