title: Na+ -Ca2+ exchanger expression and its modulation.
abstract: Here we reviewed our recent work on the chronic effects of nicotine on the Na+ -Ca2+ exchanger (NCX) gene and protein expressions in various organs of rats treated with nicotine in the drinking water for 4-12 weeks. Microarray analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) did not detect significant changes in NCX mRNA expression in cerebral cortex, hippocampus, heart and skeletal muscle. However, NCX1 protein was up-regulated by nicotine in cerebral cortex and hippocampus, but was down-regulated in the heart. NCX2 protein was up-regulated by nicotine in hippocampus. We suggest that although mRNA change was insignificant, NCX protein expression was altered by chronic nicotine administration in brain and heart in rats. We also reviewed our work on modulators of NCX gene expression and function in cardiac myocytes.
authors.affiliation: Department of Pharmacology, Fukushima Medical University, School of Medicine, Japan. jkimura@fmu.ac.jp
authors.forename: Junko
authors.initials: J
authors.lastname: Kimura
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ono
authors.forename: Kazuho
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.forename: Sachiko
authors.initials: S
authors.lastname: Maeda
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Shikama
authors.forename: Midori Sasaki
authors.initials: MS
authors.lastname: Yatabe
authors.forename: Isao
authors.initials: I
authors.lastname: Matsuoka
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D019831:Sodium-Calcium Exchanger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D019831:Sodium-Calcium Exchanger; D009538:Nicotine