Comparison of 8-isoprostane and interleukin-8 in induced sputum and exhaled breath condensate from asymptomatic and symptomatic smokers.
BACKGROUND
Markers of airway inflammation and oxidative stress have been mainly investigated in moderate/severe chronic obstructive pulmonary disease (COPD) or during its exacerbation. They have not been compared in noninvasive specimens such as exhaled breath condensate (EBC) and induced sputum in healthy nonsymptomatic smokers or in those who have symptoms and are at risk for COPD development.


OBJECTIVE
To compare the relative proportions of 2 potential COPD biomarkers, 8-isoprostane and interleukin- 8 (IL-8) in the induced sputum and EBC sampled from the same subjects: nonsmokers (n = 14), healthy smokers (n = 17) and symptomatic smokers (n = 9) who are considered to be at risk for COPD. COPD patients with acute exacerbation (n = 10) were employed as positive controls.


METHODS
The levels of the aforementioned biomarkers in induced sputum and EBC were investigated using commercial biochemical techniques.


RESULTS
In induced sputum, the levels of 8-isoprostane and IL-8 were at least 10-fold higher compared to EBC levels in all groups. Healthy nonsmokers had the lowest levels, and patients with exacerbation of COPD the highest levels of 8-isoprostane in induced sputum and EBC. The same observation held true for IL-8 in induced sputum. Inverse correlations with lung function parameters were observed for both mediators.


CONCLUSIONS
The levels of both potential markers were clearly higher in the induced sputum than in EBC. The results point to an advantage of induced sputum over EBC for assessing the degree of airway oxidative stress and inflammation in smokers with a potential risk for COPD development.
Mazur
Witold
W
Biomedicum Helsinki and Department of Internal Medicine, Division of Pulmonary Medicine, University of Helsinki and Helsinki University Central Hospital, FI-00014 Helsinki, Finland. witold.mazur@helsinki.fi
Stark
Harri
H
Sovijärvi
Anssi
A
Myllärniemi
Marjukka
M
Kinnula
Vuokko L
VL
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism*; D013183:Sputum / Q000378:metabolism*
D015415:Biomarkers; D016209:Interleukin-8; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost