Rapid detection of Salmonella in foods using real-time PCR.
Conventional methods for detection of Salmonella serovars in foods are generally time-consuming and labor intensive. A real-time PCR method has been developed with custom designed primers and a TaqMan probe to detect the presence of a 262-bp fragment of the Salmonella-specific invA gene. The method has been tested with a total of 384 field-isolated Salmonella serovars and non-Salmonella stock strains, as well as 420 U.S. Food and Drug Administration food samples, comprising a variety of food matrices. The method was highly specific in detecting Salmonella in spiked chili powder and shrimp samples, with a sensitivity of 0.04 CFU/g. In addition, the method is faster, more accurate, and less costly than the traditional U.S. Food and Drug Administration's Bacteriological Analytical Manual cell-culturing and the AOAC International-approved VIDAS methods to detect Salmonella in foods.
Cheng
Chorng-Ming
CM
U.S. Food and Drug Administration, Pacific Regional Laboratory Southwest, Irvine, California 92612, USA. chorng-ming.cheng@fda.hhs.gov
Lin
Wen
W
Van
Khanh Thien
KT
Phan
Lieuchi
L
Tran
Nelly N
NN
Farmer
Doris
D
D001426:Bacterial Proteins; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005784:Gene Amplification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019779:Salmonella enterica / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity; D013997:Time Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C075518:invA protein, Bacteria