Alpha-amylase production by Streptomyces erumpens MTCC 7317 in solid state fermentation using response surface methodology (RSM).
Production of alpha-amylase under solid state fermentation by Streptomyces erumpens MTCC 7317 has been investigated using different agro-industrial residues, i.e. cassava bagasse, sugarcane bagasse and wheat bran; wheat bran was found to be the best substrate. Among different nitrogen source supplemented to wheat bran, beef extract or peptone (1%) showed maximum enzyme production. Response surface methodology was used to evaluate the effect of main process parameters as incubation period (48 h), moisture holding capacity (70%), pH (7.0) and temperature (50 degrees C) on enzyme production by applying a full factorial central composite design. The maximum hydrolysis of soluble starch (90%) and cassava starch (75%) was obtained with the application of 4 ml (approximately 12096 U) of S. erumpens crude enzyme after 5 h of incubation.
Kar
Shaktimay
S
Microbiology Laboratory, Central Tuber Crops Research Institute (Regional Centre), Orissa, India.
Ray
Ramesh C
RC
Mohapatra
Uma B
UB
D019149:Bioreactors; D002482:Cellulose / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D002365:Manihot / Q000378:metabolism; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism; D031786:Saccharum / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors; D000516:alpha-Amylases / Q000096:biosynthesis*
D004043:Dietary Fiber; D002482:Cellulose; D013213:Starch; C027433:bagasse; D000516:alpha-Amylases; D009584:Nitrogen