Hepatitis C virus genotype in blood donors and associated liver disease in Indonesia.
OBJECTIVE
The aim of this study was to investigate the distribution of hepatitis C virus (HCV) genotype and the possible association between genotype and HCV-associated liver disease in Indonesia.


METHODS
32 anti-HCV-positive asymptomatic carriers (AC), 55 chronic hepatitis (CH), 41 liver cirrhosis (LC), and 35 hepatocellular carcinoma (HCC) patients were included in this study. HCV genotyping was performed by phylogenetic analysis of the NS5B and 5'-UTR regions.


RESULTS
The HCV subtype 1b (36.5%), based on NS5B region, was the most prevalent, followed by subtypes 3k (15.4%), 2a (14.4%), 1a (12.5%) and 1c (12.5%), and 2e (4.8%). Subtypes 2f, 3a, 3b, and 4a were also found in some of the samples. HCV subtypes 3k (40.0%) and 1a (35.0%) were the two major subtypes in AC. HCV subtype 1b was not found in AC, but it was common in CH (31.3%), LC (50.0%), and HCC (57.1%).


CONCLUSIONS
HCV subtype 1b was prevalent in samples of HCV-associated liver disease patients, including CH, LC and HCC. The percentage of subtype 1b was increased with the disease severity (AC < CH < LC < HCC).
Utama
Andi
A
Molecular Epidemiology Division, Mochtar Riady Institute for Nanotechnology, Lippo Karawaci, Tangerang, Indonesia. autama@mrinstitute.org
Budiarto
Bugi Ratno
BR
Monasari
Dewi
D
Octavia
Theresia Imelda
TI
Chandra
Ivan Stevanus
IS
Gani
Rino Alvani
RA
Hasan
Irsan
I
Sanityoso
Andri
A
Miskad
Upik Anderiani
UA
Yusuf
Irawan
I
Lesmana
Laurentius Adrianus
LA
Sulaiman
Ali
A
Tai
Susan
S
D001782:Blood Donors; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D007214:Indonesia; D008107:Liver Diseases / Q000175:diagnosis / Q000821:virology*; D010802:Phylogeny; D017361:Viral Nonstructural Proteins / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase
D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase