Preparation of a glycan library using a variety of glycosyltrasferases.
We have comprehensively cloned genes associated with the synthesis of glycans in the human body, then translated those glycogenes into recombinant glycosyltransferases using a variety of expression systems. The library of glycans or glycopeptides having various structures can be synthesized in a test tube using various enzymes. Using the glycan library, we have recently constructed a multistage tandem mass (MS( n )) spectral library containing observed spectra. This library may be utilized as a novel tool for glycomics research, as it enables users to identify oligosaccharides very easily and quickly by spectral matching.
Ito
Hiromi
H
Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Open Space Laboratory C-2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan.
Kameyama
Akihiko
A
Sato
Takashi
T
Narimatsu
Hisashi
H
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016695:Glycosyltransferases / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000302:isolation & purification* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods
D011134:Polysaccharides; D054852:Small Molecule Libraries; D016695:Glycosyltransferases