Inclusion of riboflavin in beta-cyclodextrin: a fluorimetric and absorption spectrometric study.
Formation of inclusion complexes between riboflavin and beta-cyclodextrin (beta-CD) with both 1:1 and 1:2 stoichiometry has been established by fluorimetric titration. However, in absorption spectrometric experiment, spectral change of riboflavin in the visible range could be observed only by taking beta-CD at a much higher concentration (about 100 times) than riboflavin and under such condition only 1:2 complexes could be detected. Its formation constant (K) was determined by a multiple linear regression analysis of the absorption data. The reliability of the K value was confirmed by the consistency achieved on analyzing the data at two different wavelengths.
Roy
Dalim Kumar
DK
Department of Chemistry, The University of Burdwan, Golapbag, W.B. 713104, India.
Deb
Nipamanjari
N
Ghosh
Bankim Chandra
BC
Mukherjee
Asok K
AK
D000327:Adsorption; D005470:Fluorometry; D015394:Molecular Structure; D012256:Riboflavin / Q000737:chemistry*; D013053:Spectrophotometry; D017186:Titrimetry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D047392:beta-Cyclodextrins; C031215:betadex; D012256:Riboflavin