Low decorin expression along with inherent activation of ERK1,2 in ear lobe keloids.
Over production of collagen and its abnormal assembly is the hallmark of abnormal scars such as keloids, a condition which reflects fibrosis of the skin. Fibrotic conditions of organs such as liver, lungs and eyes are characterized by low levels of decorin, a member of the small leucine proteoglycan family, which is an indispensable modulator of collagen assembly. Involvement of decorin in keloids is not well described, therefore, its expression in keloids was studied here. We showed low decorin levels in the total tissue extract of keloids as compared to normal skin. Further, we showed that this low level is due to reduced transcription of decorin by keloid fibroblasts. As decorin can also act as an intermediate signaling molecule involved in the ERK1,2 pathway, levels of both ERK1,2 and its activated counterpart were studied and found to be up-regulated in keloids. Through this study a novel attempt has been made to understand the implications of decorin expression and a possible cause of over production of collagen and its aberrant assembly in keloids is suggested.
Meenakshi
Janakiraman
J
Biomaterials Division, Central Leather Research Institute, Adyar, Chennai 600 020, India. mjanakiraman@yahoo.com
Vidyameenakshi
Sankarasubramaniam
S
Ananthram
Devarajan
D
Ramakrishnan
Karimpat Mathangi
KM
Jayaraman
Venkatraman
V
Babu
Mary
M
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D058575:Decorin; D054644:Ear Auricle; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014945:Wound Healing / Q000502:physiology*; D055815:Young Adult
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D003094:Collagen; D019950:Mitogen-Activated Protein Kinase 1