title: A cell strain cloned from Spodoptera exigua cell line (IOZCAS-Spex-II) highly susceptible to S. exigua nucleopolyhedrovirus infection.
abstract: A cell strain (IOZCAS-Spex-II-A) cloned from IOZCAS-Spex-II, a cell line established from the fat body of Spodoptera exigua (Lepidoptera: Noctuidae) larva, was characterized, and its capability to produce S. exigua nucleopolyhedrovirus was high with infection rate exceeding 90% compared with its parental cell line IOZCAS-Spex-II that scored only 50%. Growth curve of budded virus (BV) in the strain was analyzed and the titer of BV reached the highest of 3.7 x 10(4) pfu/mL by 96 h after inoculation. Concentration of occlusion bodies (OBs) produced by the cloned cell strain (IOZCAS-Spex-II-A) was 7.1 x 10(7) OBs/mL, while the parental cell line produced 2.4 x 10(7) OBs/mL. The average yield of the virus was 176 OBs/cell of IOZCAS-Spex-II-A compared with 211 OBs/cell that of the parental cell line. Significant differences were observed in virus production, growth characters, cell shape, between the parental cell line, and its clone. The cell lines (IOZCAS-Spex-II and IOZCAS-Spex-II-A) were also susceptible to Autographa californica multiple nucleopolyhedrovirus infection. In addition, they were characterized with regard to their growth rates and DNA amplification fingerprinting technique employing polymerase chain reaction.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datunlu, Beijing, 100101, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Yong-an
authors.initials: YA
authors.lastname: Zhang
authors.forename: Qilian
authors.initials: Q
authors.lastname: Qin
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Miao
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Wang
authors.forename: Liangjian
authors.initials: L
authors.lastname: Qu
authors.forename: Aijun
authors.initials: A
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002999:Clone Cells; D004198:Disease Susceptibility; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000502:physiology*; D016133:Polymerase Chain Reaction; D018411:Spodoptera / Q000166:cytology* / Q000821:virology*