Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome.
Atypical protein kinase C (aPKC) is a central component of the evolutionarily conserved Par3-Par6-aPKC complex, one of the fundamental regulators of cell polarity. We recently demonstrated that these proteins interact with Neph-nephrin molecules at the slit diaphragm of the glomerular filtration barrier. Here, we report that podocyte-specific deletion of aPKClambda/iota in mice results in severe proteinuria, nephrotic syndrome, and death at 4 to 5 wk after birth. Podocyte foot processes of knockout mice developed structural defects, including mislocalization of the slit diaphragm. In the glomerulus, aPKClambda/iota was primarily expressed in developing glomerular epithelial cells and podocyte foot processes. Interestingly, under physiologic conditions, aPKClambda/iota translocated from the apical surface to the basolateral side of developing podocytes, and this translocation preceded the development of foot processes and formation of slit diaphragms. Supporting a critical role for aPKClambda/iota in the maintenance of slit diaphragms and podocyte foot processes, aPKClambda/iota associated with the Neph-nephrin slit diaphragm complex and localized to the tips of filopodia and leading edges of cultured podocytes. These results suggest that aPKC signaling is fundamental to glomerular maintenance and development.
Huber
Tobias B
TB
Renal Division, University Hospital Freiburg, Breisacher Strasse 66, D-79106 Freiburg, Germany. tobias.huber@uniklinik-freiburg.de
Hartleben
Björn
B
Winkelmann
Kirstin
K
Schneider
Lisa
L
Becker
Jan U
JU
Leitges
Michael
M
Walz
Gerd
G
Haller
Hermann
H
Schiffer
Mario
M
D000818:Animals; D016764:Cell Polarity / Q000502:physiology*; D003643:Death; D017353:Gene Deletion; D007527:Isoenzymes / Q000172:deficiency; D007678:Kidney Glomerulus / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D018345:Mice, Knockout; D009404:Nephrotic Syndrome / Q000201:enzymology / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D050199:Podocytes / Q000201:enzymology / Q000502:physiology*; D011493:Protein Kinase C / Q000172:deficiency*; D011507:Proteinuria / Q000201:enzymology / Q000235:genetics*; D000099293:Protein Kinase C-lambda
D007527:Isoenzymes; D011493:Protein Kinase C; D000099293:Protein Kinase C-lambda; C115015:PKC-3 protein