Adapting to contour-clamped homogeneous electric field minichamber technology the PulseNet protocols to resolve XbaI-DNA fragments of Salmonella serotype Braenderup.
We propose cost-effective protocols for preparing and resolving the PulseNet universal DNA size standard in contour-clamped homogeneous electric field (CHEF) minichambers. Intact DNA molecules were prepared with protease-free solutions, and electrophoresis separations of the DNA standards needed 5.5h, giving band pattern resolutions similar to those attained with the PulseNet protocols standardized in CHEF chambers.
Lopez-Canovas
Lilia
L
Molecular Biology Department, Cuban Neuroscience Center, Havana, Cuba. llc@cneuro.edu.cu
Riveron
Ana Maria
AM
Garrido
Yainelis
Y
Corrales
Fidel
F
Aguila
Adalberto
A
Cárdenas
Yamilet
Y
D004269:DNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D015203:Reproducibility of Results; D012475:Salmonella / Q000145:classification / Q000235:genetics*; D012703:Serotyping
D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific