Three-dimensional visualization of a human chromosome using coherent X-ray diffraction.
Coherent x-ray diffraction microscopy is a lensless phase-contrast imaging technique with high image contrast. Although electron tomography allows intensive study of the three-dimensional structure of cellular organelles, it has inherent difficulty with thick objects. X rays have the unique benefit of allowing noninvasive analysis of thicker objects and high spatial resolution. We observed an unstained human chromosome using coherent x-ray diffraction. The reconstructed images in two or three dimensions show an axial structure, which has not been observed under unstained conditions.
Nishino
Yoshinori
Y
RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Takahashi
Yukio
Y
Imamoto
Naoko
N
Ishikawa
Tetsuya
T
Maeshima
Kazuhiro
K
D002877:Chromosomes, Human / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000379:methods*; D014961:X-Ray Diffraction / Q000379:methods*