Purification, crystallization and preliminary X-ray diffraction of wild-type and mutant recombinant human transforming growth factor beta-induced protein (TGFBIp).
Transforming growth factor beta-induced protein (TGFBIp) has been linked to several corneal dystrophies as certain point mutations in the protein may give rise to a progressive accumulation of insoluble protein material in the human cornea. Little is known about the biological functions of this extracellular protein, which is expressed in various tissues throughout the human body. However, it has been found to interact with a number of extracellular matrix macromolecules such as collagens and proteoglycans. Structural information about TGFBIp might prove to be a valuable tool in the elucidation of its function and its role in corneal dystrophies caused by mutations in the TGFBI gene. A simple method for the purification of wild-type and mutant forms of recombinant human TGFBIp from human cells under native conditions is presented here. Moreover, the crystallization and preliminary X-ray analysis of TGFBIp are reported.
Runager
Kasper
K
Center for Insoluble Protein Structures and Interdisciplinary Nanoscience Center at the Department of Molecular Biology, University of Aarhus, Denmark.
García-Castellanos
Raquel
R
Valnickova
Zuzana
Z
Kristensen
Torsten
T
Nielsen
Niels Chr
NC
Klintworth
Gordon K
GK
Gomis-Rüth
F Xavier
FX
Enghild
Jan J
JJ
D002846:Chromatography, Affinity; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D050505:Mutant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000302:isolation & purification*; D000099222:betaIG-H3 Protein
D016326:Extracellular Matrix Proteins; D050505:Mutant Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein