title: [Effect of atorvastatin on lipopolysaccharide-induced expression of C-reactive protein in human pulmonary epithelial cells].
abstract: OBJECTIVE
  To determine the effect of atorvastatin on lipopolysaccharide-induced expression of C-reactive protein in cultured A549 cells.
  
  
  METHODS
  A549 cells were incubated in DMEM medium containing lipopolysaccharide in the absence or presence of various concentrations of atorvastatin. After the incubation, the medium was collected and the level of C-reactive protein was measured by enzyme-linked immunosorbent assay. The cells were harvested and C-reactive protein mRNA was analyzed by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Incubation with lipopolysaccharide significantly induced a time and dose dependent increase in the mRNA expression and the production of C-reactive protein in A549 cells (P<0.05). Atorvastatin significantly decreased the lipopolysaccharide induced the mRNA expression and the production of C-reactive protein in a dose dependent manner in A549 cells (P<0.05).
  
  
  CONCLUSIONS
  Atorvastatin downregulates lipopolysaccharide-induced expression of C-reactive protein in cultured A549 cells, which may be its mechanism of anti-inflammation.
authors.affiliation: Department of Respiratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. wu_shangjie@hotmail.com
authors.forename: Shangjie
authors.initials: S
authors.lastname: Wu
authors.forename: Xiqian
authors.initials: X
authors.lastname: Xing
authors.forename: Ye
authors.initials: Y
authors.lastname: Gan
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
mesh_terms: D000069059:Atorvastatin; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D011758:Pyrroles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D006538:Heptanoic Acids; D008070:Lipopolysaccharides; D011758:Pyrroles; D012333:RNA, Messenger; D002097:C-Reactive Protein; D000069059:Atorvastatin