[Determination of melamine in milk powder and milk by high performance liquid chromatography].
The analytical method of melamine in milk and milk powder by high performance liquid chromatography (HPLC) was established. The sample was purified by a cation exchange solid-phase extraction column, separated on a Diamonsil C18 column (4.6 mm x 200 mm, 5 microm) with acetonitrile-0.01 mol/L sodium heptanesulfonate (pH 3.3) (10:90, v/v) as mobile phase at a flow rate of 1.0 mL/min, and determined by a photodiode array detector. A linear calibration curve was obtained in the concentration range of 1 - 500 mg/L. The qualification limit was 0.2 mg/kg, and the quantification limit was 1 mg/kg. The recovery was 81.4% -83.7%.
He
Qiaosang
Q
Zhejiang Test Academy of Quality and Technical Supervision, Hangzhou 310013, China. hqsang@163.com
Liu
Minfang
M
Huang
Liying
L
Yang
Yuanyuan
Y
Liao
Shangfu
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D041943:Infant Formula / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D011208:Powders; D014227:Triazines / Q000032:analysis* / Q000302:isolation & purification
D011208:Powders; D014227:Triazines; C011907:melamine