Intercellular exchanges of membrane fragments (trogocytosis) between human muscle cells and immune cells: a potential mechanism for the modulation of muscular immune responses.
Trogocytosis is a cell-contact dependent intercellular transfer of membrane fragments and associated molecules. We studied trogocytosis in the interaction of T cells with human skeletal muscle cells modeling muscle-immune cell interactions under pathophysiological conditions i.e. myositis. Human myoblasts donate membrane fragments to T cells. Acquisition of muscle-derived membrane molecules depended on T-cell activation, was independent of T-cell receptor engagement, sensitive to inhibition of actin polymerization and amplified by protein kinase C activation. Single-cell patch clamping was used to demonstrate the change in membrane capacitance upon incorporation of membrane fragments in T cells. Membrane uptake was fast and temporarily, but had clear functional consequences: T cells after intimate contact with myoblasts stimulated the proliferation of autologous T cells. Our observations raise the hypothesis that trogocytosis may modulate the outcome of T-T interactions within the micromilieu of skeletal muscle.
Waschbisch
Anne
A
Clinical Research Group for Multiple Sclerosis and Neuroimmunology, Department of Neurology, University of Wuerzburg, Wuerzburg, Germany.
Meuth
Sven G
SG
Herrmann
Alexander M
AM
Wrobel
Barbara
B
Schwab
Nicholas
N
Lochmüller
Hanns
H
Wiendl
Heinz
H
D015551:Autoimmunity / Q000502:physiology; D002450:Cell Communication / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008564:Membrane Potentials / Q000276:immunology; D018482:Muscle, Skeletal / Q000276:immunology* / Q000503:physiopathology; D032446:Myoblasts / Q000166:cytology / Q000276:immunology; D009220:Myositis / Q000276:immunology* / Q000503:physiopathology*; D018408:Patch-Clamp Techniques; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013347:Subcellular Fractions / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D011948:Receptors, Antigen, T-Cell