Nexin-1 inhibits the activity of human brain trypsin.
Trypsin and other trypsin-like serine proteases have been shown to play important roles in neural development, plasticity and neurodegeneration. Their activity is modulated by serine protease inhibitors, serpins. However, for human brain trypsin, trypsin-4, no brain-derived inhibitors have been described. Here, we report that nexin-1 inhibits trypsin-4, and forms stable complexes only with this trypsin-isoenzyme. This result suggests that nexin-1 could modulate trypsin activity in brain where both nexin-1 and trypsin-4 are expressed.
Koistinen
H
H
Department of Clinical Chemistry, Biomedicum, University of Helsinki and Helsinki University Central Hospital, PO Box 63, FIN 00014, Finland. hannu.k.koistinen@helsinki.fi
Koistinen
R
R
Zhang
W-M
WM
Valmu
L
L
Stenman
U-H
UH
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D058255:Protease Nexins; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000378:metabolism
D016564:Amyloid beta-Protein Precursor; D007527:Isoenzymes; D058255:Protease Nexins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D014361:Trypsin Inhibitors; C040749:PRSS3 protein, human; D014357:Trypsin