A truncated T antigen expressed from an alternatively spliced BK virus early mRNA.
The early region of BK virus (BKV) is known to encode two well-characterized tumour (T) antigens, large T antigen (TAg) and small T antigen (tAg). In this study, we provide evidence of a third early BKV mRNA that codes for an additional early region product with an apparent molecular mass of 17-20 kDa. This truncated form of TAg (truncTAg) is expressed from an alternatively spliced mRNA that is derived from the excision of a second intron from the mRNA encoding TAg. The first 133 aa of truncTAg are identical to those of TAg but the additional splice results in translation from a different reading frame, adding three new amino acids before reaching a stop codon. TruncTAg is expressed in both BKV-transformed and lytically infected cells and it is found to be primarily localized to the nucleus. The function of BKV truncTAg is likely to be relevant to transformation, similar to the additional T antigens of simian virus 40, JC virus and mouse polyomavirus.
Abend
Johanna R
JR
Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109-5942, USA.
Joseph
Amy E
AE
Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109-5942, USA.
Das
Dweepanita
D
Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109-5942, USA.
Campbell-Cecen
Deniz B
DB
Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109-5942, USA.
Imperiale
Michael J
MJ
Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109-5942, USA.
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism*; D001739:BK Virus / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D021381:Protein Transport / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
D000957:Antigens, Viral, Tumor; D012333:RNA, Messenger; D012367:RNA, Viral