Syntheses of NPTX-594 analogs with thiol-containing fluorophores to develop a probe for analysis of binding mode between spider toxins and glutamate receptors.
NPTX-594 is a neurotoxic acylpolyamine isolated from a Madagascar Joro spider and acts as a specific blocker of glutamate receptors (GluRs). The present study reports the creation of novel NPTX-594 analogs with thiol-containing fluorophores for use as visualization probes for the analysis of the binding mode between spider toxins and GluRs, together with the result of their cricket bioassay.
Nishimaru
Takahiro
T
Faculty of Science and Technology, Kinki University, Higashi-osaka, Osaka 577-8502, Japan.
Yamaguchi
Yoshihiro
Y
Wakamiya
Tateaki
T
D000818:Animals; D001681:Biological Assay; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D011073:Polyamines / Q000138:chemical synthesis* / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism; D013112:Spiders / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis*
D005456:Fluorescent Dyes; C540561:NPTX-594; D011073:Polyamines; D017470:Receptors, Glutamate; D013438:Sulfhydryl Compounds