title: Relationship between recurrent vulvovaginal candidosis and immune mediators in vaginal fluid.
abstract: OBJECTIVE
  Recurring vulvovaginal candidosis (RVVC) is a common vaginal discharge, affecting 75% of all women at least once in their life. In 5% of these women, this infection is recurring. Recent studies have focused on determining the importance of vaginal-associated immunity. To investigate the effects of immune mediators and the probability of an immune defect as well as localized allergic responses due to candida, we examined the cytokines IL-4, IL-5, IL-13 as well as prostaglandin E(2) (PgE(2)), candida-specific IgE and total-IgE in patients with a clinical diagnosis of RVVC.
  
  
  METHODS
  In 104 symptomatic and 41 asymptomatic patients, regarding clinical symptoms of RVVC, vaginal fluids were measured by ELISA for the immune mediators IL-4, IL-5, IL-13, PgE(2), candida-specific IgE and total-IgE. Polymerase chain reactions as well as culture were used for candida detection.
  
  
  RESULTS
  Concerning IL-5, IL-13 and total-IgE no statistically significant difference was found. Those women with a history of RVVC and who were currently symptomatic had elevated concentrations of IL-4 (p<.0001), PgE(2) (p<.0001) and candida-specific IgE (p<0.02) in their vaginal fluids compared to women with no clinical symptoms. Those three interleukins showed no significant differences between symptomatic patients who were positive or negative for Candida albicans or Candida glabrata.
  
  
  CONCLUSIONS
  These findings indicate that women with clinical vaginal symptoms have a localized vaginal immunosuppression which might be the cause of their symptoms. Greatly elevated vaginal PgE(2) levels can be a consequence of a localized allergic response. An allergic reaction to candida components may be the cause of the allergic response in those women with high levels of candida-specific IgE. In women with elevated PgE(2), treatment with an antihistamine or prostaglandin synthesis inhibitor might be beneficial.
authors.affiliation: Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Weissenbacher
authors.forename: Steven S
authors.initials: SS
authors.lastname: Witkin
authors.forename: Andrea
authors.initials: A
authors.lastname: Gingelmaier
authors.forename: Christoph
authors.initials: C
authors.lastname: Scholz
authors.forename: Klaus
authors.initials: K
authors.lastname: Friese
authors.forename: Ioannis
authors.initials: I
authors.lastname: Mylonas
mesh_terms: D001826:Body Fluids / Q000378:metabolism*; D002181:Candidiasis, Vulvovaginal / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D006633:Histamine Antagonists / Q000627:therapeutic use; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007165:Immunosuppression Therapy; D018793:Interleukin-13 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D012008:Recurrence; D014621:Vagina / Q000378:metabolism*
chemical_list: D016861:Cyclooxygenase Inhibitors; D006633:Histamine Antagonists; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4; D007073:Immunoglobulin E; D015232:Dinoprostone