title: [Establish stable cell line to express M2 ion channel of influenza A virus H5N1].
abstract: The M2 ion channel protein is an important target against influenza A virus. In this study, H5N1 influenza A virus M2 ion channel (H5M2) gene was cloned into pcDNA4 vector. The HEK293 stable cell line expressing H5M2 was successfully established. The expression of H5M2 ion channel protein was induced only by tetracycline and confirmed by imuunofluorescence and Western blot. The ion channel activity of H5M2 was confirmed by whole cell patch-clamp recording. Fifty micromol per liter amantadine blocked the H5M2 channel conductance completely in HEK293 cells. This stable cell line may provide a model for screening inhibitors of M2 ion channel.
authors.affiliation: Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences (GIBH), Guangzhou 510663, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Sun
authors.forename: Chufang
authors.initials: C
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Li
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000378:metabolism*; D007473:Ion Channels / Q000037:antagonists & inhibitors; D007668:Kidney / Q000166:cytology*; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*; D000086082:Viroporin Proteins
chemical_list: D007473:Ion Channels; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins