A Method for Isolation of Pasteuria penetrans Endospores for Bioassay and Genomic Studies.
A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations.
Waterman
Jenora T
JT
Center for the Biology of Nematode Parasitism, NC State University, Raleigh, NC 27695.
Bird
David McK
DM
Opperman
Charles H
CH