title: Screening of antioxidant phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC.
abstract: The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentration-dependent antioxidative capacity was verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results obtained indicate that the in vitro production of B. oleracea var. costata shoots can become important in the obtention of a noticeable dietary source of compounds with health protective potential.
authors.affiliation: REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: David M
authors.initials: DM
authors.lastname: Pereira
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Marcos
authors.initials: M
authors.lastname: Taveira
authors.forename: Anabela
authors.initials: A
authors.lastname: Martins
authors.forename: José A
authors.initials: JA
authors.lastname: Pereira
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Seabra
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds; D001937:Brassica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D006997:Hypochlorous Acid; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010851:Picrates; D018520:Plant Shoots / Q000737:chemistry; D013481:Superoxides; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D013481:Superoxides; D006997:Hypochlorous Acid; C004931:1,1-diphenyl-2-picrylhydrazyl