title: Hepatocyte growth factor exerts promoting functions on murine dental papilla cells.
abstract: Hepatocyte growth factor (HGF) is a potent mitogenic and angiogenic factor involved in the development of many tissues including the teeth. We previously reported that dental pulp cells express the HGF receptor c-met and that HGF can enhance cell proliferation and differentiation. The purpose of this study was to determine whether HGF could stimulate proliferation and/or differentiation of murine dental papilla cells (MDPCs). MDPCs were isolated from neonatal mice, and the mitogenic potential of HGF on MDPCs was measured by 3-(4, 5-dimethyl-thyazol-2-yl)-2, 5-diphenyltetrazolium and flow cytometry. Differentiation of MDPCs in the presence of HGF was characterized by mineralization assay, alkaline phosphatase (ALP) activity and reverse-transcription polymerase chain reaction. We reported that MDPCs expressed both HGF and c-met and that HGF enhanced proliferation of MDPCs by concentrations ranging from 5 ng/mL to 20 ng/mL, with 10 ng/mL as the optimal concentration (p < 0.05). HGF prolonged S phase and shortened G1 phase of MDPCs, increased ALP activity significantly over the control (p < 0.05), and enhanced the formation of mineral nodules. ALP, dentin sialophosphoprotein, and dentin matrix protein-1 expression were significantly increased in the presence of HGF (p < 0.05), as were the expression of Msx1, Runx2, and Pax9. Our data suggest that HGF plays an important role in tooth development, by promoting the proliferation and differentiation of MDPCs.
authors.affiliation: West China School of Stomatology, Sichuan University, Sichuan, China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.forename: Libin
authors.initials: L
authors.lastname: Ren
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism*; D003810:Dentinogenesis / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D016193:G1 Phase / Q000187:drug effects; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D051957:MSX1 Transcription Factor / Q000096:biosynthesis; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008938:Mitosis; D051764:PAX9 Transcription Factor; D051761:Paired Box Transcription Factors / Q000096:biosynthesis; D010750:Phosphoproteins; D011498:Protein Precursors / Q000096:biosynthesis; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase / Q000187:drug effects; D012795:Sialoglycoproteins; D014074:Tooth Calcification; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D050659:Core Binding Factor Alpha 1 Subunit; D016326:Extracellular Matrix Proteins; D017228:Hepatocyte Growth Factor; D051957:MSX1 Transcription Factor; D051764:PAX9 Transcription Factor; D051761:Paired Box Transcription Factors; D010750:Phosphoproteins; D011498:Protein Precursors; D019859:Proto-Oncogene Proteins c-met; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513308:Dmp1 protein, mouse; C484084:Msx1 protein, mouse; C488759:Pax9 protein, mouse; C493716:Runx2 protein, mouse