Fluorescence Quenching/Enhancement Surface Assays: Signal Manipulation Using Silver-coated Gold Nanoparticles.
Gold nanoparticles covalently attached to the indium tin oxide coated glass slide drastically quench fluorescence of a surface immunoassay (approximately 5-fold). Silver electrochemically deposited over the gold particles leads to fluorescence amplification: signal increases approximately 7-8 times if compared to the signal on gold particles not covered with silver. This phenomenon allows enhancing of the surface immunoassays utilizing both types of nanoparticles.
Matveeva
Evgenia G
EG
Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology; Department of Cell Biology and Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76106, USA.
Shtoyko
Tanya
T
Gryczynski
Ignacy
I
Akopova
Irina
I
Gryczynski
Zygmunt
Z