title: Substrate specificity in phospholipid transformations by plant phospholipase D isoenzymes.
abstract: Phospholipase D (PLD) catalyzes the hydrolysis and transesterification of glycerophospholipids at the terminal phosphodiester bond. In many plants, several isoforms of PLD have been identified without knowing their functional differences. In this paper, the specificities of two PLD isoenzymes from white cabbage (Brassica oleracea var. capitata) and two ones from opium poppy (Papaver somniferum L.), which were recombinantly produced in Escherichia coli, were compared in the hydrolysis of phospholipids with different head groups and in the transphosphatidylation of phosphatiylcholine with several acceptor alcohols. In a biphasic reaction system, consisting of buffer and diethyl ether, the highly homologous isoenzymes are able to hydrolyze phosphatidylcholine, -glycerol, -ethanolamine, -inositol and - with one exception - also phosphatidylserine but with different individual reaction rates. In transphosphatidylation of phosphatidylcholine, they show significant differences in the rates of head group exchange but with the same trend in the preference of acceptor alcohols (ethanolamine>glycerol>>l-serine). For l- and d-serine a stereoselectivity of PLD was observed. The results suggest a physiological relevance of the different hydrolytic and transphosphatidylation activities in plant PLD isoenzymes.
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther University, Halle-Wittenberg, Kurt-Mothes-Str. 3, D-06099 Halle, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Dippe
authors.forename: Renate
authors.initials: R
authors.lastname: Ulbrich-Hofmann
mesh_terms: D001937:Brassica / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D010207:Papaver / Q000201:enzymology; D010739:Phospholipase D / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D010743:Phospholipids; D010940:Plant Proteins; D010739:Phospholipase D