title: [Effects of PUVA on mitochondrial membrane potential in HL-60 and K562 cells].
abstract: OBJECTIVE
  To study the effects of psoralen (PSO) and long wave ultraviolet light (PUVA) on mitochondrial membrane potential in HL-60 and K562 cells.
  
  
  METHODS
  Cells were incubated with PSO in different concentrations irradiated with or without UVA. The changes of ultrastructure of cells were observed under the electron microscope. The apoptosis ratios and the changes of mitochondrial membrane potential were detected through the flow cytometry. The factorial design and analysis of variance were used to analyze the interaction among the factors.
  
  
  RESULTS
  There were obvious ultrastructure changes about apoptosis in HL-60 and K562 cells after treated with PUVA. PSO, UVA and PUVA all increased the apoptosis ratios and decreased the mitochondrial membrane potential, and the effects of PUVA were stronger than the other two (p<0.01).
  
  
  CONCLUSIONS
  PUVA can induce the apoptosis of HL-60 and K562 cells and one of the pathway to induce apoptosis is to downregulate the mitochondrial membrane potential.
authors.affiliation: Department of Traditional Chinese Medicine and Hematology, 210th Hospital of PLA, Dalian 116021, China. chnngzx@163.com
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Chen
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Liu
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: De-Jie
authors.initials: DJ
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015536:Down-Regulation; D005434:Flow Cytometry; D011564:Furocoumarins / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects* / Q000502:physiology; D011701:PUVA Therapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D011564:Furocoumarins; D017319:Photosensitizing Agents