[The influence of lysogenic phage on biofilm formation of Pseudomonas aeruginosa].
OBJECTIVE
To investigate the effect of the lysogenic phage Ppa3094 on the biofilm formation of PA3094.


METHODS
The modified plate culture method was used to established the biofilm model in vitro. The viable counts of bacteria in biofilm were detected by MTT method; The real-time RT-PCR was applied to measure the expression level of algC and algD during the biofilm formtion of PA3094 and PA3094-L.


RESULTS
Biofilm of both strains were mature at 5th to 7th day. The structures of the biofilms were both like pellicle. There was a significant difference in the viable counts of bacteria during biofilm development between PA3094 and PA3094-L. The expression of algC and algD genes was upregulated during biofilm formation. However, the expression level of PA3094-L was lower than PA3094, especially algC at 12 h.


CONCLUSIONS
The lysogenic phage Ppa3094 could influence the biofilm formation during its development through changing the expressing level of the alginate biosynthetic genes.
Du
Bao-zhong
BZ
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zeng
Wei
W
Tao
Chuan-min
CM
Kang
Mei
M
Liu
Mei
M
Zhang
Zai-rong
ZR
Jia
Wen-xiang
WX
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001435:Bacteriophages / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000821:virology*
D001426:Bacterial Proteins