title: Efficient detection of QTL with large effects in a simulated pig-type pedigree using selective genotyping.
abstract: BACKGROUND
  The ultimate goal of QTL studies is to find causative mutations, which requires additional expression studies. Given the limited amount of time and funds, the smart option is to identify the most important QTL with minimal effort. A cost-effective solution is to genotype only those animals with high or low phenotypic values or DNA-pools of these individuals. A two-stage genotyping strategy was applied on samples in the tails of the distribution of breeding values.
  
  
  RESULTS
  The tail-analysis approach identified eight out of the 19 QTL in the first stage, explaining about half of 98% of the genetic variance. Four additional QTL with small effects were found in the second stage.
  
  
  CONCLUSIONS
  The two-stage genotyping strategy with selective genotyping detected regions with highly significant QTL useful for further fine-mapping. The large reduction in costs allows for follow-up expression and functional studies.
authors.affiliation: Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, P,O, box 80163, 3508 TD Utrecht, The Netherlands. h.c.m.heuven@uu.nl
authors.forename: Henri C M
authors.initials: HC
authors.lastname: Heuven
authors.forename: John W M
authors.initials: JW
authors.lastname: Bastiaansen
authors.forename: Stéphanie M
authors.initials: SM
authors.lastname: van den Berg