title: Flow-injection chemiluminescence method for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride.
abstract: A sensitive and simple flow-injection chemiluminescence (FI-CL) method, which was based on the CL intensity generated from the redoxreaction of potassium permanganate (KMnO4)-formaldehyde in vitriol (H2SO4) medium, has been developed, validated and applied for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride. Besides oxidants and sensitizers, the effect of the concentration of H(2)SO(4), KMnO4 and formaldehyde was investigated. Under the optimum conditions, the linear range was 1.0 x 10(-2)-7.0 mg/L for naphazoline hydrochloride and 5.0 x 10(-2)-10.0 mg/L for oxymetazoline hydrochloride. During seven repeated inter-day and intra-day precision tests of 0.1, 1.0 and 10.0 mg/L samples, the relative standard deviations all corresponded to reference values. The detection limit was 8.69 x 10(-3) mg/L for naphazoline hydrochloride and 3.47 x 10(-2) mg/L for oxymetazoline hydrochloride (signal-to-noise ratio < or = 3). This method has been successfully implemented for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride in pharmaceuticals.
authors.affiliation: Department of Medicament, The Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, People's Republic of China. nami_wan@163.com
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Wang
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Shao
authors.forename: Yu-Hai
authors.initials: YH
authors.lastname: Tang
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Yin
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Wu
mesh_terms: D002138:Calibration; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005557:Formaldehyde / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D009278:Naphazoline / Q000032:analysis*; D010109:Oxymetazoline / Q000032:analysis*; D011196:Potassium Permanganate / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D011196:Potassium Permanganate; D005557:Formaldehyde; D010109:Oxymetazoline; D009278:Naphazoline