title: Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells.
abstract: Feeding experiments using [1-(13)C]-d-glucose to Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by (13)C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space.
authors.affiliation: Division of Pharmacognosy, Leiden University, Einsteinweg 55, Leiden, The Netherlands.
authors.forename: Natali R
authors.initials: NR
authors.lastname: Mustafa
authors.forename: Hye Kyong
authors.initials: HK
authors.lastname: Kim
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.forename: Cornelis
authors.initials: C
authors.lastname: Erkelens
authors.forename: Alfons W M
authors.initials: AW
authors.lastname: Lefeber
authors.forename: Gerwin
authors.initials: G
authors.lastname: Spijksma
authors.forename: Robert
authors.initials: R
authors.lastname: van der Heijden
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D002247:Carbon Isotopes; D030008:Catharanthus / Q000378:metabolism* / Q000382:microbiology; D002478:Cells, Cultured; D062385:Hydroxybenzoates / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D011775:Pythium / Q000502:physiology*; D020156:Salicylic Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D062385:Hydroxybenzoates; C009135:2,3-dihydroxybenzoic acid; D020156:Salicylic Acid