The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation.
Signal transducer and activator of transcription 3 (STAT3), which is activated by cytokines and growth factors, mediates biological actions in many physiological processes. In a previous study, we found that Y14, a core component of the exon-junction complex (EJC) bound to STAT3 and upregulated the transcriptional activity of STAT3 by influencing its DNA-binding activity. In the present study, we demonstrate that STAT3 endogenously interacts with Y14. In addition, we found that MAGOH, a Y14 partner in the EJC, inhibits the STAT3-Y14 complex formation. Furthermore, small-interfering RNA-mediated reduction of MAGOH expression enhanced interleukin-6-induced gene expression. These results indicate that MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and Y14.
Muromoto
Ryuta
R
Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo 060-0812, Japan.
Taira
Naohisa
N
Ikeda
Osamu
O
Shiga
Kaname
K
Kamitani
Shinya
S
Togi
Sumihito
S
Kawakami
Shiho
S
Sekine
Yuichi
Y
Nanbo
Asuka
A
Oritani
Kenji
K
Matsuda
Tadashi
T
D005091:Exons; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015533:Transcriptional Activation
C491494:MAGOH protein, human; D009687:Nuclear Proteins; C406243:RBM8A protein, human; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human