title: Lgl2 and E-cadherin act antagonistically to regulate hemidesmosome formation during epidermal development in zebrafish.
abstract: The integrity and homeostasis of the vertebrate epidermis depend on various cellular junctions. How these junctions are assembled during development and how their number is regulated remain largely unclear. Here, we address these issues by analysing the function of Lgl2, E-cadherin and atypical Protein kinase C (aPKC) in the formation of hemidesmosomes in the developing basal epidermis of zebrafish larvae. Previously, we have shown that a mutation in lgl2 (penner) prevents the formation of hemidesmosomes. Here we show that Lgl2 function is essential for mediating the targeting of Integrin alpha 6 (Itga6), a hemidesmosomal component, to the plasma membrane of basal epidermal cells. In addition, we show that whereas aPKClambda seems dispensable for the localisation of Itga6 during hemidesmosome formation, knockdown of E-cadherin function leads to an Lgl2-dependent increase in the localisation of Itga6. Thus, Lgl2 and E-cadherin act antagonistically to control the localisation of Itga6 during the formation of hemidesmosomes in the developing epidermis.
authors.affiliation: Max-Planck Institut für Entwicklungsbiologie, Department of Genetics, Spemannstrasse 35, Tuebingen, D-72076, Germany. mahendra.sonawane@tuebingen.mpg.de
authors.forename: Mahendra
authors.initials: M
authors.lastname: Sonawane
authors.forename: Hans
authors.initials: H
authors.lastname: Martin-Maischein
authors.forename: Heinz
authors.initials: H
authors.lastname: Schwarz
authors.forename: Christiane
authors.initials: C
authors.lastname: Nüsslein-Volhard
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000254:growth & development* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D022002:Hemidesmosomes / Q000235:genetics / Q000378:metabolism*; D039503:Integrin alpha6 / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D011493:Protein Kinase C / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D028961:beta Karyopherins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D039503:Integrin alpha6; D007527:Isoenzymes; D028961:beta Karyopherins; C424163:late gestation lung 2 karyopherin; D011493:Protein Kinase C