title: Characterization of a CD44/CD122int memory CD8 T cell subset generated under sterile inflammatory conditions.
abstract: Most memory CD8 T cell subsets that have been hitherto defined are generated in response to infectious pathogens. In this study, we have characterized the CD8 T cells that survive priming conditions, devoid of pathogen-derived danger signals. In both a TCR-transgenic model and a model of contact hypersensitivity, we show that the priming of naive CD8 T cells under sterile inflammatory conditions generates memory. The corresponding memory CD8 T cells can be identified by their intermediate expression levels of CD44 and CD122. We also show that CD44/122(int) memory CD8 T cells spontaneously develop in wild type mice and that they display intermediate levels of several other memory traits including functional (IFN-gamma secretion capacity, CCL5 messenger stores), phenotypic, and molecular (T-bet and eomesodermin expression levels) features. We finally show that they correspond to an early differentiation stage and can further differentiate in CD44/122(high) memory T cells. Altogether, our results identify a new memory CD8 T cell subset that is generated under sterile inflammatory conditions and involved in the recall contact hypersensitivity reactions that are responsible for allergic contact dermatitis.
authors.affiliation: Université de Lyon, France.
authors.forename: Florentin-Martial
authors.initials: FM
authors.lastname: Mbitikon-Kobo
authors.forename: Marc
authors.initials: M
authors.lastname: Vocanson
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Michallet
authors.forename: Martine
authors.initials: M
authors.lastname: Tomkowiak
authors.forename: Anne
authors.initials: A
authors.lastname: Cottalorda
authors.forename: Georgi S
authors.initials: GS
authors.lastname: Angelov
authors.forename: Charles-Antoine
authors.initials: CA
authors.lastname: Coupet
authors.forename: Sophia
authors.initials: S
authors.lastname: Djebali
authors.forename: Antoine
authors.initials: A
authors.lastname: Marçais
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dubois
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonnefoy-Bérard
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicolas
authors.forename: Christophe
authors.initials: C
authors.lastname: Arpin
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000502:physiology*; D007156:Immunologic Memory / Q000235:genetics*; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology*; D053646:Interleukin-2 Receptor beta Subunit / Q000096:biosynthesis / Q000502:physiology*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers; C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; C508022:Il2rb protein, mouse; D018836:Inflammation Mediators; D053646:Interleukin-2 Receptor beta Subunit