Transcriptional responses of Mycobacterium tuberculosis to lung surfactant.
This study uses microarray analyses to examine gene expression profiles for Mycobacterium tuberculosis (Mtb) induced by exposure in vitro to bovine lung surfactant preparations that vary in apoprotein content: (i) whole lung surfactant (WLS) containing the complete mixture of endogenous lipids and surfactant proteins (SP)-A, -B, -C, and -D; (ii) extracted lung surfactant (CLSE) containing lipids plus SP-B and -C; (iii) column-purified surfactant lipids (PPL) containing no apoproteins, and (iv) purified human SP-A. Exposure to WLS evoked a multitude of transcriptional responses in Mtb, with 52 genes up-regulated and 23 genes down-regulated at 30min exposure, plus 146 genes up-regulated and 27 genes down-regulated at 2h. Notably, WLS rapidly induced several membrane-associated lipases that presumptively act on surfactant lipids as substrates, and a large number of genes involved in the synthesis of phthiocerol dimycocerosate (PDIM), a cell wall component known to be important in macrophage interactions and Mtb virulence. Exposure of Mtb to CLSE, PPL, or purified SP-A caused a substantially weaker transcriptional response (</=20 genes were induced) suggesting that interactions among multiple lipid-protein components of WLS may contribute to its effects on Mtb transcription.
Schwab
Ute
U
Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA. ues3@cornell.edu
Rohde
Kyle H
KH
Wang
Zhengdong
Z
Chess
Patricia R
PR
Notter
Robert H
RH
Russell
David G
DG
D000818:Animals; D002417:Cattle; D020869:Gene Expression Profiling; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D011663:Pulmonary Surfactants / Q000378:metabolism*; D013312:Stress, Physiological
D011663:Pulmonary Surfactants