title: Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples.
abstract: Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
authors.affiliation: Laboratório de Biologia Molecular de Adenovírus, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-900.
authors.forename: Juliana Cristina
authors.initials: JC
authors.lastname: Marinheiro
authors.forename: Roberta Braga
authors.initials: RB
authors.lastname: Sanalios
authors.forename: Daniela Carvalho dos
authors.initials: DC
authors.lastname: Santos
authors.forename: Cristovão Alves da
authors.initials: CA
authors.lastname: Costa
authors.forename: Charlotte Marianna
authors.initials: CM
authors.lastname: Hársi
mesh_terms: D000208:Acute Disease; D000256:Adenoviridae / Q000235:genetics* / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000175:diagnosis*; D016022:Case-Control Studies; D002648:Child; D006801:Humans; D009305:Nasopharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity