[Determination of anthraquinone in Semen Cassiae from different regions by HPLC].
OBJECTIVE
To determine the content of 7 anthraquinones in Semen Cassiae.


METHODS
A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1).


RESULTS
Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different.


CONCLUSIONS
The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.
Zhang
Yi
Y
Chongqing Academy of Chinese Materia Medcia, Chongqing 400065, China.
Huang
Xiao-ping
XP
Weng
Dai-qun
DQ
Yang
Da-jian
DJ
D000880:Anthraquinones / Q000032:analysis*; D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal