Regulation of episomal gene expression by KRAB/KAP1-mediated histone modifications.
KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studied within the environment of chromosomal DNA. Here we demonstrate that KRAB/KAP1 regulation is fully functional within the context of episomal DNA, such as adeno-associated viral and nonintegrated lentiviral vectors, and is correlated with histone modifications typically associated with this epigenetic regulator.
Barde
Isabelle
I
School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Laurenti
Elisa
E
Verp
Sonia
S
Groner
Anna Claire
AC
Towne
Christopher
C
Padrun
Viviane
V
Aebischer
Patrick
P
Trumpp
Andreas
A
Trono
Didier
D
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006657:Histones / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D000076183:Tripartite Motif-Containing Protein 28
D004268:DNA-Binding Proteins; D006657:Histones; D012097:Repressor Proteins; C422407:ZNF350 protein, human; D004247:DNA; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28