Molecular characterization of an atoxigenic Aspergillus flavus strain AF051.
An atoxigenic Aspergillus flavus strain AF051 collected from a peanut field in Jiangsu province, P. R. China was characterized by analysis of aflatoxin gene cluster in this study. By using a thermal asymmetric interlaced PCR (TAIL-PCR) and conventional PCR techniques, an 89.59-kb deletion was found in the cluster, and this deletion was replaced by a 3.83-kb insert, which was located at 300-bp upstream ver1 gene and 2594-bp downstream a putative gluconolactone oxidase gene. Based on the DNA sequence at the breakpoint, a nested-PCR method was developed for the rapid and sensitive detection of AF051 strain in soil and peanut samples once the strain is used as a biological agent.
Jiang
Jinhua
J
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
Yan
Leiyan
L
Ma
Zhonghua
Z
D000348:Aflatoxins / Q000235:genetics* / Q000378:metabolism; D001231:Aspergillus flavus / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017384:Sequence Deletion
D000348:Aflatoxins; D005656:Fungal Proteins