Isolation and characterization of extracellular polymeric substances from micro-algae Dunaliellasalina under salt stress.
Extracellular polymeric substances (EPSs), produced by Dunaliella salina strain, increase concomitantly with salt concentration and maximum (944 mg/l) were obtained at 5M NaCl, whereas minimum (56 mg/l) at 0.5M salinity. Emulsifying activity was measured in terms of strength to retain the emulsion and comparatively 85.76% retention was observed at 0.5M salinity thereafter it intends to decline. The FT-IR-spectra reveal characteristic functional groups NH stretching, asymmetrical CH stretching vibration of aliphatic CH(2)-group, CC stretching of aromatic, CN stretch of aliphatic amine, NH wag of primary amine and CX stretch of alkyl-halides with a stretching of COC, CO corresponding to the presence of carbohydrates. The FT-IR-spectra substantiated the presence of primary amine-group, aromatic-compound, halide-group, aliphatic alkyl-group and polysaccharides. Four monosaccharides (glucose, galactose, fructose and xylose) were also detected by HPLC analysis. Production of EPSs may allow further exploration of D. salina as potential EPSs producer and make it as a promising candidate for biotechnological and industrial exploitation.
Mishra
Avinash
A
Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research, GB Marg, Bhavnagar, Gujarat, India. avinash@csmcri.org
Jha
Bhavanath
B
D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D045703:Emulsifying Agents / Q000737:chemistry; D056890:Eukaryota / Q000378:metabolism*; D005632:Fructose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D009005:Monosaccharides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014994:Xylose / Q000032:analysis
D045703:Emulsifying Agents; D009005:Monosaccharides; D011108:Polymers; D011134:Polysaccharides; D012492:Salts; D005632:Fructose; D014994:Xylose; D005947:Glucose; D005690:Galactose