title: Arrested growth of Trypanosoma cruzi by the calpain inhibitor MDL28170 and detection of calpain homologues in epimastigote forms.
abstract: In this paper, we aimed to explore the effects of the calpain inhibitor III (MDL28170) and to detect calpain-like molecules (CALPs) in epimastigote forms of Trypanosoma cruzi isolate Dm28c. MDL28170 at 70 microM promoted a powerful reduction in the growth rate after 48 h. The IC50 value was calculated to be 31.7 microM. This inhibitor promoted an increase in the cellular volume, but not cell lysis, resulting in a trypanostatic effect. T. cruzi CALPs presented a strong cross-reactivity with anti-Drosophila melanogaster calpain and anti-cytoskeleton-associated protein from Trypanosoma brucei antibodies, and labelling was found mainly intracellularly. Furthermore, an 80 kDa reactive protein was detected by Western blotting assays. No significant cross-reactivity was found with anti-human brain calpain antibody. The expression of CALPs was decreased in cells kept for long periods in axenic cultures in comparison to a strain recently isolated from mice, as well as in MDL28170-treated cells, the latter being paralleled by an increased expression of cruzipain. Different levels of CALPs expression were also detected in distinct phylogenetic lineages, like Y strain (lineage TcII), Dm28c (lineage TcI) [corrected] and INPA6147 strain (Z3 zymodeme). These results may contribute for the investigation of the functions of CALPs in trypanosomatids.
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Sangenito
authors.forename: V
authors.initials: V
authors.lastname: Ennes-Vidal
authors.forename: F A
authors.initials: FA
authors.lastname: Marinho
authors.forename: F F
authors.initials: FF
authors.lastname: Da Mota
authors.forename: A L S
authors.initials: AL
authors.lastname: Santos
authors.forename: C M
authors.initials: CM
authors.lastname: D'Avila-Levy
authors.forename: M H
authors.initials: MH
authors.lastname: Branquinha
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D002154:Calpain / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D004151:Dipeptides / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D015853:Cysteine Proteinase Inhibitors; D004151:Dipeptides; D015800:Protozoan Proteins; D002154:Calpain; C058076:calpain inhibitor III