title: Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV.
abstract: White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus infecting shrimp and other crustaceans. The potentiality of binary ethylenimine (BEI)-inactivated WSSV against WSSV in crayfish, Procambarus clarkii, was investigated in this study. Efficacy of BEI-inactivated WSSV was tested by vaccination trials followed by challenge of crayfish with WSSV. The crayfish injected with BEI-inactivated WSSV showed a better survival (P<0.05) to WSSV on the 7th and 21st day post-vaccination (dpv) compared to the control. Calculated relative percent survival (RPS) values were 77% and 60% on the 7th and 21st dpv for 2mM BEI-inactivated WSSV, and 63%, 30% on 7th and 21st dpv for 3mM BEI-inactivated WSSV. However, heat-inactivated WSSV did not provide protection from WSSV even on 7th dpv. In the inactivation process WSSV especially their envelope proteins maybe changed as happened to 3mM BEI and heat-inactivated WSSV particles. These results indicate the protective efficacy of BEI-inactivated WSSV lies on the integrity of envelope proteins of WSSV and the possibility of BEI-inactivated WSSV to protect P. clarkii from WSSV.
authors.affiliation: Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhu
authors.forename: Huahua
authors.initials: H
authors.lastname: Du
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Miao
authors.forename: Hai-Zhi
authors.initials: HZ
authors.lastname: Quan
authors.forename: Zi-Rong
authors.initials: ZR
authors.lastname: Xu
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000276:immunology* / Q000821:virology*; D001388:Aziridines / Q000737:chemistry; D006358:Hot Temperature; D016019:Survival Analysis; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D038301:Virus Inactivation; D046848:White spot syndrome virus 1 / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D001388:Aziridines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; C478655:binary ethyleneimine