[Effects of perfluorooctane sulfonate on amino acid neurotransmitters and glutamine synthetase in rats].
OBJECTIVE
To discuss the dose-effect of perfluorooctane sulfonate (PFOS) on excitatory amino acids (EAAs) and glutamine synthetase (GS) in rat central nerves system.


METHODS
20 mature male Wister rats were categorized into 4 groups randomly, 5 rats every group. Wister rats were treated once by gastric perfusion method with 12.5, 25, 50 mg/kg PFOS and controls received same valume vehicle (2% tween-80) once orally. 5 days after treated, the rats in all groups were sacrificed. The contents of glutamate (Glu), aspartate (Asp), glycine (Gly) and gamma-aminobutyric acid (GABA) in brain tissues were determined by high performance liquid chromatography (HPLC), Changes in GS in brain tissue were observed GS kit.


RESULTS
The spontaneous motor ability was decreased as compared with the controls. In hippocampus, the content of glutamate (Glu) was significantly decreased at the dose of 50 mg/kg bw (P < 0.05). In cortex, the content of glutamate (Glu) was significantly decreased at the dose of 25 and 50 mg/kg bw (P < 0.05). In hippocampus, increase with the dose of PFOS there was significant difference of GS at 12.5 and 50 mg/kg group compared with corresponding control group (P < 0.05).


CONCLUSIONS
The altered content of amino acid neurotransmitters might be one of the important mechanisms of PFOS neurotoxicity.
Yang
Xiaoshi
X
Department of Social Medicine, China Medical University, Shengyang 110001, China.
Wang
Lie
L
Sun
Wei
W
Xue
Zhongli
Z
D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity; D018846:Excitatory Amino Acids / Q000378:metabolism*; D005466:Fluorocarbons / Q000633:toxicity*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D017738:Alkanesulfonic Acids; D004785:Environmental Pollutants; D018846:Excitatory Amino Acids; D005466:Fluorocarbons; D018377:Neurotransmitter Agents; C076994:perfluorooctane sulfonic acid; D005974:Glutamate-Ammonia Ligase