Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection.
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
Kühn
Jonas
J
Institute of Imaging and Applied Optics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. jonas.kuehn@a3.epfl.ch
Montfort
Frédéric
F
Colomb
Tristan
T
Rappaz
Benjamin
B
Moratal
Corinne
C
Pavillon
Nicolas
N
Marquet
Pierre
P
Depeursinge
Christian
C
D002477:Cells / Q000648:ultrastructure*; D004867:Equipment Design; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D006696:Holography / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*