title: Adapting to contour-clamped homogeneous electric field minichamber technology the PulseNet protocols to resolve XbaI-DNA fragments of Salmonella serotype Braenderup.
abstract: We propose cost-effective protocols for preparing and resolving the PulseNet universal DNA size standard in contour-clamped homogeneous electric field (CHEF) minichambers. Intact DNA molecules were prepared with protease-free solutions, and electrophoresis separations of the DNA standards needed 5.5h, giving band pattern resolutions similar to those attained with the PulseNet protocols standardized in CHEF chambers.
authors.affiliation: Molecular Biology Department, Cuban Neuroscience Center, Havana, Cuba. llc@cneuro.edu.cu
authors.forename: Lilia
authors.initials: L
authors.lastname: Lopez-Canovas
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Riveron
authors.forename: Yainelis
authors.initials: Y
authors.lastname: Garrido
authors.forename: Fidel
authors.initials: F
authors.lastname: Corrales
authors.forename: Adalberto
authors.initials: A
authors.lastname: Aguila
authors.forename: Yamilet
authors.initials: Y
authors.lastname: Cárdenas
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D015203:Reproducibility of Results; D012475:Salmonella / Q000145:classification / Q000235:genetics*; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific