Amyloid fibrillation of hen egg-white lysozyme is inhibited by TCEP.
This work examines the inhibitory effect of TCEP on the in vitro fibrillation of hen lysozyme at pH 2. We demonstrate that the inhibition of hen lysozyme fibrillation by TCEP follows a dose-dependent manner. Our data show that the addition of TCEP prevents alpha-to-beta transition and promoted unfolding of lysozyme. Moreover, our findings suggested that the TCEP-induced attenuated fibrillation is associated with disulfide disruption and structural unfolding of HEWL.
Wang
Steven S-S
SS
Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan. sswang@ntu.edu.tw
Liu
Kuan-Nan
KN
Lu
Yen-Chun
YC
D000682:Amyloid / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D010720:Phosphines / Q000737:chemistry / Q000494:pharmacology*; D017433:Protein Structure, Secondary / Q000187:drug effects
D000682:Amyloid; D009113:Muramidase; D010720:Phosphines; C080938:tris(2-carboxyethyl)phosphine; C421864:hen egg lysozyme