title: Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
abstract: Although numerous studies have shown a higher risk of acquiring HIV infection in the presence of other sexually transmitted diseases, the biologic mechanisms responsible for enhanced HIV acquisition are unclear. Because Langerhans cells (LCs) are suspected to be the initial HIV targets after sexual exposure, we studied whether microbial components augment HIV infection in LCs by activating Toll-like receptor (TLR) and nucleotide-binding oligomerization domain (NOD) pattern recognition receptors. We found that TLR1/2 and TLR2/6 agonists dramatically enhanced both HIV susceptibility and replication in immature monocyte-derived LCs, whereas TLR3-5, TLR7-9, and NOD1,2 agonists did not significantly affect HIV infection. The same infection-enhancing effects were observed when LCs were incubated with other related bacterial components as well as with whole Gram(+) bacteria. In resident LCs in human skin, TLR2 agonists also significantly increased HIV susceptibility. By contrast, TLR2 agonists and related bacterial components decreased HIV susceptibility in monocyte-derived dendritic cells (DCs). We found that TLR2 activation of LCs, but not DCs, resulted in a significant down-regulation of APOBEC3G, which is a cellular restriction factor for HIV. Given these data, we hypothesize that ligation of TLR2 by Gram(+) bacterial products may underlie enhanced sexual transmission of HIV that occurs with concomitant bacterial sexually transmitted disease infections.
authors.affiliation: Department of Dermatology, University of Yamanashi, Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Tatsuyoshi
authors.initials: T
authors.lastname: Kawamura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ito
authors.forename: Andrew
authors.initials: A
authors.lastname: Blauvelt
authors.forename: Shinji
authors.initials: S
authors.lastname: Shimada
mesh_terms: D000071480:APOBEC-3G Deaminase; D003564:Cytidine Deaminase / Q000235:genetics; D003713:Dendritic Cells / Q000821:virology*; D004198:Disease Susceptibility / Q000209:etiology*; D015536:Down-Regulation; D006094:Gram-Positive Bacteria / Q000276:immunology*; D015658:HIV Infections / Q000209:etiology*; D006801:Humans; D007113:Immunity, Innate; D007801:Langerhans Cells / Q000821:virology*; D053472:Nod Signaling Adaptor Proteins / Q000819:agonists / Q000378:metabolism; D012749:Sexually Transmitted Diseases / Q000209:etiology; D012867:Skin / Q000166:cytology; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000819:agonists / Q000276:immunology / Q000378:metabolism*
chemical_list: D053472:Nod Signaling Adaptor Proteins; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase