Mobile elements of an Azospirillum brasilense Sp245 85-MDa plasmid involved in replicon fusions.
Sequence analysis of approximately 25kb of an Azospirillum brasilense Sp245 85-MDa ( approximately 142kb) plasmid, p85, identified two novel IS elements mediating p85 fusions with a suicide plasmid vector, pJFF350. These IS elements, 1465-bp ISAzba1 and 1112-bp ISAzba3, belong to the IS256 family and to the IS5 family/IS903 group, respectively. Truncated ISAzba2 from the ISL3 family was found near one of the copies of ISAzba1 that flank pJFF350 in p85::pJFF350. As another factor potentially contributing to the known genetic plasticity of p85, a phage integrase gene was identified in this plasmid.
Katsy
Elena I
EI
Laboratory of Microbial Genetics, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, 410049 Saratov, Russia. katsy@ibppm.sgu.ru
Prilipov
Alexei G
AG
D000595:Amino Acid Sequence; D015806:Azospirillum brasilense / Q000235:genetics*; D001483:Base Sequence; D019426:Integrases / Q000235:genetics; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D012093:Replicon / Q000235:genetics*; D016415:Sequence Alignment
D019426:Integrases