AhpC (alkyl hydroperoxide reductase) from Anabaena sp. PCC 7120 protects Escherichia coli from multiple abiotic stresses.
Alkyl hydroperoxide reductase (AhpC) is known to detoxify peroxides and reactive sulfur species (RSS). However, the relationship between its expression and combating of abiotic stresses is still not clear. To investigate this relationship, the genes encoding the alkyl hydroperoxide reductase (ahpC) from Anabaena sp. PCC 7120 were introduced into E. coli using pGEX-5X-2 vector and their possible functions against heat, salt, carbofuron, cadmium, copper and UV-B were analyzed. The transformed E. coli cells registered significantly increase in growth than the control cells under temperature (47 degrees C), NaCl (6% w/v), carbofuron (0.025mgml(-1)), CdCl(2) (4mM), CuCl(2) (1mM), and UV-B (10min) exposure. Enhanced expression of ahpC gene as measured by semi-quantitative RT-PCR under aforementioned stresses at different time points demonstrated its role in offering tolerance against multiple abiotic stresses.
Mishra
Yogesh
Y
Center of Advanced Study in Botany, Banaras Hindu University, Varanasi, India.
Chaurasia
Neha
N
Rai
Lal Chand
LC
D017033:Anabaena / Q000201:enzymology*; D019256:Cadmium Chloride / Q000494:pharmacology; D003001:Cloning, Molecular; D003300:Copper / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D015870:Gene Expression; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D003300:Copper; D054464:Peroxiredoxins; D019256:Cadmium Chloride