Altered flow-mediated vasodilatation, low paraoxonase-1 activity, and abnormal high-density lipoprotein subclass distribution in Takayasu's arteritis.
BACKGROUND
Takayasu's arteritis (TA) is an idiopathic chronic inflammatory disease that causes occlusion of large arteries, but little is known about whether affected patients are characterized by endothelial dysfunction, different high-density lipoproteins (HDL) subclasses and paraoxonase-1 (PON1) activity.


RESULTS
In the present study, 30 patients with TA, 30 age- and gender-matched volunteers (controls) and 15 patients with essential hypertension were studied. Flow-mediated vasodilation (FMD) and maximal blood flow velocity, assessed in the brachial artery by high-resolution ultrasound, were significantly lower in patients. HDL subclass distribution was determined by polyacrylamide gradient gel electrophoresis. HDL-cholesterol, HDL3b subclass and PON1 activity, assessed spectrophotometrically using phenylacetate as the substrate, were also lower in patients compared with controls. In a multiple regression analysis, the use of prednisone and systolic blood pressure were independent variables that predicted the FMD.


CONCLUSIONS
A low FMD, abnormal size distribution of HDLs, and low PON1 activity are observed in TA patients. These abnormalities appear independently and constitute a cluster that may contribute to the vascular dysfunction of TA arteritis.
Espinola-Zavaleta
Nilda
N
Echocardiography in Out Patient Clinic, Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Soto-López
María Elena
ME
Carreón-Torres
Elizabeth
E
Gamboa
Ricardo
R
Mejía
Ana M
AM
Márquez-Velasco
Ricardo
R
Bojalil
Rafael
R
Huesca-Gomez
Claudia
C
Reyes
Pedro A
PA
Pérez-Méndez
Oscar
O
D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D001783:Blood Flow Velocity; D005260:Female; D006801:Humans; D053440:Lipoproteins, HDL3 / Q000097:blood*; D008875:Middle Aged; D013625:Takayasu Arteritis / Q000097:blood* / Q000503:physiopathology; D014664:Vasodilation
D053440:Lipoproteins, HDL3; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human