Monomeric pheophorbide(a)-containing poly(ethyleneglycol-b-epsilon-caprolactone) micelles for photodynamic therapy.
Incorporation of unaggregated monomeric molecules of pheophorbide(a) into micelles of poly(ethyleneoxide-b-epsilon-caprolactone) has been characterized by fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy and asymmetric flow field flow fractionation. It was shown that the method used leads to 20 nm micelles, corresponding to approximately 200 molecules of polymer and 4 molecules of monomeric pheophorbide(a) per nano-object which was able to generate (1)O(2) in the medium. They have been used thereafter as nanocarriers for photosensitizers in photodynamic therapy against cancer cells. The encapsulation of photosensitizer has been verified and in vitro tests on human cancerous cells have revealed a ca. 2-fold enhanced photocytotoxicity and cellular uptake compared to free pheophorbide(a).
Knop
Katrin
K
Université de Toulouse; UPS/CNRS, IMRCP, 118 route de Narbonne, F-31062, Toulouse Cedex 9, France.
Mingotaud
Anne-Françoise
AF
El-Akra
Naram
N
Violleau
Frédéric
F
Souchard
Jean-Pierre
JP
D045744:Cell Line, Tumor; D002734:Chlorophyll / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D004339:Drug Compounding; D005026:Ethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000633:toxicity; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry
D005026:Ethylene Glycols; D008823:Micelles; D017319:Photosensitizing Agents; D011091:Polyesters; C476300:poly(epsilon-caprolactone)-b-poly(ethylene glycol); D002734:Chlorophyll; D026082:Singlet Oxygen; C032623:pheophorbide a