title: Studies of various biochemical parameters of rat plasma following chronic administration of "Rohitakarista"-an Ayurvedic formulation.
abstract: The study was carried out to investigate the safety profile as well as the effect of "Rohitakarista" (RHT) on various biochemical parameters of rats' plasma after chronic administration. RHT, a classical Ayurvedic preparation used in hepatosplenic disorders, was administered per oral route at a dose of 100 mg kg(-1) body weight, once daily, up to 46 days for all the experiments. Forty albino rats (Rattus novergicus: Sprague-Dawley strains), equally of both sexes, were randomly grouped into four where each group had ten animal/sex. One male and one female group were used as control and other groups were used as test. In the male, rats there was a statistically insignificant increase (p = 0.763) in the total protein but there was a statistically significant increase (p = 0.022) in the total protein content of the plasma of female rats. Statistically very high significant increase (male: p = 0.001 and female: p = 0.001) in the albumin content of the plasma was noted in both sexes. In case of bilirubin, interestingly it was decreased very high significantly (p = 0.001) in plasma of male rats but increased very high significantly (p = 0.001) in the plasma of female rats. In the male rats, statistically there was a very high significant decrease (sGPT: p = 0.001, sGOT: p = 0.001 and ALP: p = 0.001) in the sGPT, sGOT and ALP activities in the plasma. On the other hand, statistically there was a very highly significant increase (sGPT: p = 0.001, sGOT: p = 0.001 and ALP: p = 0.001) in the sGPT, sGOT and ALP activities in the plasma of female rats. Very high significant decrease (male: p = 0.001 and female: p = 0.001) in creatinine in plasma of both sexes were observed after chronic administration of RHT. Urea in the plasma was decreased very high significantly (p = 0.001) in plasma of male rats but increased very high significantly (p = 0.001) in the plasma of female rats. There was high significant increase (p = 0.002) in uric acid in male rats. On the contrary, no significant increase (p = 0.324) of uric acid was observed in female rats.
authors.affiliation: Department of Pharmacy, Southeast University, Banani, Dhaka-1213, Bangladesh.
authors.forename: M Obayed
authors.initials: MO
authors.lastname: Ullah
authors.forename: M Jashim
authors.initials: MJ
authors.lastname: Uddin
authors.forename: K
authors.initials: K
authors.lastname: Hamid
authors.forename: S
authors.initials: S
authors.lastname: Kabir
authors.forename: M Azizur
authors.initials: MA
authors.lastname: Rahman
authors.forename: M S K
authors.initials: MS
authors.lastname: Choudhuri
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005260:Female; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000502:physiology; D008297:Male; D008515:Medicine, Ayurvedic; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors