Simultaneous determination of nine flavonoids in Anaphalis margaritacea by capillary zone electrophoresis.
A capillary zone electrophoresis method was developed for simultaneous determination of nine flavonoids, including two rare flavonols, in Tibetan medicine Anaphalis margaritacea. Baseline separation was performed at pH 9.6 with 25 mM Na(2)B(4)O(7) and 10 mM NaH(2)PO(4) buffer solution, 20 kV as driving voltage and 275 nm as detection wavelength. Repeatability tests showed that the R.S.D. of both intra- and inter-day migration times and peak areas were less than 5%. Recovery results ranged from 87.9% to 106.1%. Samples of A. margaritacea extracts were analyzed using the validated method, which is useful for its quality control.
Ren
Zhao-Yan
ZY
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Zhang
Yu
Y
Shi
Yan-Ping
YP
D019659:Asteraceae; D019075:Electrophoresis, Capillary / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results
D005419:Flavonoids; D007202:Indicators and Reagents; D010936:Plant Extracts