[Determination of eleven steroid hormones in animal muscle tissues and eggs using ultra-performance liquid chromatography-tandem mass spectrometry].
A credible method was developed for the simultaneous determination of eleven steroid hormone residues in animal muscle tissues and eggs based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The eleven steroid hormones were testosterone, methyltestosterone, trenbolone, boldenone, nandrolone, methandienone, stanozolol, progesterone, nadrolone propionate, testosterone propionate and nadrolone phenylpropionate. The samples were extracted with tert-butyl methyl ether at alkaline pH and then cleaned up by freezing-lipid filtration. All these drugs can be assayed in 10 min by UPLC-MS/MS using electrospray ionization in positive ion mode and multiple reaction monitoring mode. The limits of detection were 0.3 microg/kg for testosterone, methyltestosterone, boldenone, methandienone and stanozolol, and 0.4 microg/kg for trenbolone nandrolone, progesterone, testosterone propionate and nadrolone phenylpropionate. Overall recoveries of testosterone, methyltestosterone, boldenone, methandienone and stanozolol were 62.3% - 105% from pork, beef, mutton and chicken muscle tissues, and eggs fortified at the 1, 2 and 10 microg/kg levels, and the relative standard deviations (RSDs) were 0.5% - 15%. The recoveries of trenbolone nandrolone, progesterone, testosterone propionate and nadrolone phenylpropionate were higher than 50.0%, and the RSDs were lower than 16%. The matrix calibration curve for each drug was linear (r > 0.99) from 1 to 100 microg/L. The established method is simple, rapid, sensitive and specific, and is appropriate for the identification and quantification of anabolic androgenic steroids in animal muscle tissues and eggs.
He
Limin
L
Department of Pharmacology and Toxicology, College of Veterinary Medicine, South China Agriculture University, Guangzhou 510642, China. liminokhe@scau.edu.cn
Huang
Xianhui
X
Fang
Binghu
B
Huang
Shixin
S
Cao
Ying
Y
Chen
Jianxin
J
Zeng
Zhenling
Z
Chen
Zhangliu
Z
D053000:Analytic Sample Preparation Methods; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis*; D006728:Hormones / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D008460:Meat / Q000032:analysis; D009132:Muscles / Q000737:chemistry / Q000166:cytology*; D013256:Steroids / Q000032:analysis* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
D006728:Hormones; D013256:Steroids