[Extraction, isolation and immunological identification of allergens from Pseudosciaena crocea].
OBJECTIVE
To extract, identify and purify the major allergens of Pseudosciaena crocea in order to evaluate the immunological activities.


METHODS
Pseudosciaena crocea proteins were extracted by Coca's buffer. Allergens of the Pseudosciaena crocea were identified by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western-Blotting. Major allergens were isolated by ion exchange chromatagraphy (IEC) and their immunological activities were evaluated by Western-Blotting with the serum of patients as 1st antibody.


RESULTS
The molecular weight of the Pseudosciaena crocea protein were between 8-116 kD. The molecular weight of the major allergens of Pseudosciaena crocea were 54, 29, 27, 14 kD. The concentration of allergens of Pseudosciaena crocea after isolated by IEC were highly improved and the immunological activities were kept.


CONCLUSIONS
The allergens of Pseudosciaena crocea were detected and identified by immunological activity.
Yang
Rui
R
Allergy and Immunology Institute, Shenzhen University, Shenzhen 510860, China. yangruimail@yahoo.com.cn
Wu
Haiqiang
H
Liu
Zhigang
Z
D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D010473:Perciformes / Q000276:immunology* / Q000378:metabolism
D000485:Allergens; D029941:Fish Proteins; D007073:Immunoglobulin E