title: [Peptide TAT modified polyethylenimine-beta-cyclodextrin for gene delivery].
abstract: To develop a novel gene delivery vector TAT-PEI-beta-CyD.
  
  
  
  beta-cyclodextrin (beta-CyD) was linked by low molecular weight (PEI 600) via 1, 1-carbonyldiimidazole (CDI), and TAT peptide (RRRQRRKKRC) was coupled to PEI 600 by [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. The copolymer was characterized by (1)H-NMR and FT-IR. Physiochemical characteristics of TAT-PEI-beta-CyD/DNA complexes were tested by agarose gel electrophoresis and particle size measurements. Cell viability and transfection efficiency were evaluated in A293 and B16 cells using PEI 25 kDa as a control.
  
  
  
  TAT peptide was successfully coupled to PEI-beta-CyD. The result of gel electrophoresis showed that the TAT-PEI-beta-CyD was able to condense DNA efficiently at N/P ratio of 4. The particle size of TAT-PEI-beta-CyD/DNA complexes was around 100 nm. The cytotoxicity of TAT-PEI-beta-CyD was lower than that of PEI 25 kDa. The transfection efficiency of TAT-PEI-beta-CyD was higher than that of PEI 25 kDa in A293 and B16 cells at N/P ratio of 30.
  
  
  
  The novel vector TAT-PEI-beta-CyD has been developed successfully with low cytotoxicity and high transfection efficiency.
authors.affiliation: Institute of Immunology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Lai
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Jiang
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Hu
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Wang
authors.forename: Gu-Ping
authors.initials: GP
authors.lastname: Tang
mesh_terms: D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D047392:beta-Cyclodextrins; D054322:tat Gene Products, Human Immunodeficiency Virus; D011094:Polyethyleneimine; C031215:betadex