title: Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae.
abstract: A Saccharomyces cerevisiae strain deficient in phosphatidylethanolamine methyltransferase that exhibits choline auxotrophy grew in the presence of dioctanoyl phosphatidylcholine (diC8PC). We isolated and characterized mutants defective in growth in the diC8PC-containing medium. Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ono
authors.forename: Ryouichi
authors.initials: R
authors.lastname: Fukuda
authors.forename: Akinori
authors.initials: A
authors.lastname: Ohta
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010713:Phosphatidylcholines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C478771:Lem3 protein, S cerevisiae; D026901:Membrane Transport Proteins; D010713:Phosphatidylcholines; D029701:Saccharomyces cerevisiae Proteins