Antioxidants and lipid peroxidation status in diabetic patients with and without complications.
BACKGROUND
Oxidative stress is involved in the pathophysiology of diabetes mellitus.


METHODS
In the present study, 68 patients with type 2 diabetes mellitus and 31 clinically healthy individuals were evaluated. The patients were divided into two groups. Group 1 included 29 patients without diabetic complications and group 2 consisted of 39 patients with diabetic complications. Erythrocyte glutathione, superoxide dismutase, and thiobarbituric acid-reactive substance levels as well as plasma antioxidant vitamins C and E, and serum total glutathione-S-transferase, ceruloplasmin, and protein thiols were estimated by using spectro-photometer.


RESULTS
A significant decrease of erythrocyte glutathione was observed in group 1 when compared with the controls. Thiols decreased in group 2. An increase in glutathione-S-transferase, ceruloplasmin, superoxide dismutase, and vitamins C and E levels was noted in patients with diabetes mellitus. Thiobarbituric acid-reactive substance levels decreased in group 1 but increased in group 2 when compared with the controls.


CONCLUSIONS
In the present study, tendency of most of the antioxidants to rise in diabetes could probably be due to an adaptive response to the pro-oxidant milieu of the diabetic state. Hence, we suggest that supplementation with dietary antioxidants especially antioxidant vitamins accompanied by change in lifestyle might help to reduce damage brought about by free radical toxicity in diabetes mellitus.
Srivatsan
Rama
R
Department of Biochemistry, Kasturba Medical College and Hospital, Manipal-576104, Karnataka, India.
Das
Sujata
S
Gadde
Ranjita
R
Manoj-Kumar
Krishna
K
Taduri
Snigdha
S
Rao
Nageshwara
N
Ramesh
B
B
Baharani
Akanksha
A
Shah
Kaajal
K
Kamireddy
Srilakshmi C
SC
Priyatham
G
G
Balakumaran
T A
TA
Balakumaran
Shubha Seshadri
SS
Kamath
Asha
A
Rao
Anjali
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D002570:Ceruloplasmin / Q000378:metabolism; D048909:Diabetes Complications / Q000097:blood / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000097:blood / Q000378:metabolism; D055815:Young Adult
D000975:Antioxidants; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid