title: A simple assay system to monitor the potential for contamination during different stages of cryopreservation.
abstract: A simple assay to monitor the potential for contamination during different steps of cryopreservation is described. The assay is based on the contamination of liquid nitrogen using crystals of sucrose hemi-heptahydrate, these are stable in liquid nitrogen, nitrogen vapour and ambient air and can be monitored by a simple assay which allows contamination risks to be evaluated in a direct, rapid manner.
authors.affiliation: Asymptote Ltd., St John's Innovation Centre, Cowley Road, Cambridge CB4 0WS, UK. jmorris@asymptote.co.uk
authors.forename: G J
authors.initials: GJ
authors.lastname: Morris
mesh_terms: D001681:Biological Assay / Q000379:methods*; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods*; D003460:Crystallization; D004866:Equipment Contamination / Q000517:prevention & control; D009584:Nitrogen; D013395:Sucrose / Q000737:chemistry
chemical_list: D013395:Sucrose; D009584:Nitrogen