title: Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice.
abstract: K88 (F4) fimbrial adhesin, FaeG, was expressed extracellularly in Lactococcus lactis using a nisin-controlled gene expression system. The antibody response and protective efficacy of the recombinant bacteria (L. lactis [spNZ8048-faeG]) against live enterotoxigenic E. coli (ETEC) C(83549) challenge were evaluated in ICR mice. Mice vaccinated with L. lactis [spNZ8048-faeG] had a significantly increased antigen-specific IgG level in the serum and decreased mortality rate (P < 0.05) compared with the control. This indicates that oral immunization of L. lactis [spNZ8048-faeG] can induce an immune-response protection upon challenge with live ETEC in ICR mice.
authors.affiliation: Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, People's Republic of China.
authors.forename: Chun Xia
authors.initials: CX
authors.lastname: Hu
authors.forename: Zi Rong
authors.initials: ZR
authors.lastname: Xu
authors.forename: Wei Fen
authors.initials: WF
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Niu
authors.forename: Niu
authors.initials: N
authors.lastname: Dong
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Ling Lin
authors.initials: LL
authors.lastname: Fu
mesh_terms: D018830:Adhesins, Escherichia coli / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D016019:Survival Analysis
chemical_list: D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D022361:Escherichia coli Vaccines; C494388:FaeG protein, E coli; D007074:Immunoglobulin G