Effectiveness of antibiotics and antiseptics on coagulase-negative staphylococci for the decontamination of bone allografts.
Bone allografts retrieved from multi-organ donors can be decontaminated with minimally aggressive methods. Therefore, we evaluated the efficacy of antibiotics and antiseptics in the decontamination of bone fragments actively contaminated with coagulase-negative staphylococci. Gentamicin (512/1,024 microg/mL), rifampicin (400/1,000 microg/mL), chlorhexidine in alcohol and chlorhexidine soap were tested with different contact times and temperatures and a delay in starting decontamination. Gentamicin-susceptible strains dried on bone could be removed by gentamicin 512 microg/mL after 19 h of contact, while strains not dried on bone could be eliminated by soaking bone for 60 min in gentamicin 512 microg/mL. Rifampicin-susceptible strains could be eliminated by soaking bone for 60 min in rifampicin 1,000 microg/mL. In none of the experimental conditions could gentamicin/rifampicin-resistant staphylococci be eliminated. Antiseptics could not eliminate staphylococci from bone. Different antibiotics need different protocols in order to decontaminate bone allografts.
Saegeman
V S M
VS
Tissue Banks, UH-KULeuven, Herestraat 49, 3000, Leuven, Belgium.
Ectors
N L
NL
Lismont
D
D
Verduyckt
B
B
Verhaegen
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001842:Bone and Bones / Q000382:microbiology*; D003030:Coagulase / Q000096:biosynthesis; D004203:Disinfection / Q000379:methods*; D006801:Humans; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology; D013696:Temperature; D013997:Time Factors; D014184:Transplantation, Homologous
D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D003030:Coagulase