title: Postthymic maturation influences the CD8 T cell response to antigen.
abstract: Complete T cell development requires postthymic maturation, and we investigated the influence of this ontological period on the CD8 T cell response to infection by comparing responses of mature CD8 T cells with those of recent thymic emigrants (RTEs). When activated with a noninflammatory stimulus or a bacterial or viral pathogen, CD8 RTEs generated a lower proportion of cytokine-producing effector cells and long-lived memory precursors compared with their mature counterparts. Although peripheral T cell maturation is complete within several weeks after thymic egress, RTE-derived memory cells continued to express inappropriate levels of memory cell markers and display an altered pattern of cytokine production, even 8 weeks after infection. When rechallenged, RTE-derived memory cells generated secondary effector cells that were phenotypically and functionally equivalent to those generated by their mature counterparts. The defects at the effector and memory stages were not associated with differences in the expression of T cell receptor-, costimulation-, or activation-associated cell surface markers yet were associated with lower Ly6C expression levels at the effector stage. This work demonstrates that the stage of postthymic maturation influences cell fate decisions and cytokine profiles of stimulated CD8 T cells, with repercussions that are apparent long after cells have progressed from the RTE compartment.
authors.affiliation: Department of Immunology, University of Washington, Campus Box 357650, Seattle, WA 98195-7650, USA.
authors.forename: Lydia E
authors.initials: LE
authors.lastname: Makaroff
authors.forename: Deborah W
authors.initials: DW
authors.lastname: Hendricks
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Niec
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Fink
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001424:Bacterial Infections / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D016207:Cytokines / Q000096:biosynthesis; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D013997:Time Factors; D014780:Viruses / Q000276:immunology
chemical_list: D000941:Antigens; D016207:Cytokines; D011948:Receptors, Antigen, T-Cell