title: Molecular cytogenetic analysis of urothelial carcinomas using urine samples.
abstract: Urinary cells obtained from voided urine specimens of 46 patients with urothelial carcinomas (UCs) and 10 normal individuals were analyzed with 3 different centromeric fluorescence in situ hybridization (FISH) probes. The overall sensitivity of cytology was 48.9% compared to 95.7%with the FISH technique. The minimum values were found for stage Ta and grade 1 (90.5 and 89.4) and sensitivity of FISH in other stages and grades was 100%. Chromosome 3 demonstrated the most frequent chromosomal abnormality in all samples (43%), followed by chromosome 17 (32%) and chromosome 7 (25%). There was a statistically significant association between the number of cell abnormalities in chromosome 17 and the tumour stage (p value=0.02). No relationship was found between the type of chromosomal abnormality and grade. Thus feasibility and reliability of a FISH based approach was confirmed for detection of UC in urine samples.
authors.affiliation: Department of Urooncology, Imam Khomeini Hospital, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ayati
authors.forename: V Najjaran
authors.initials: VN
authors.lastname: Tousi
authors.forename: T
authors.initials: T
authors.lastname: Sabokba
authors.forename: H
authors.initials: H
authors.lastname: Vaziri-Nasab
authors.forename: F
authors.initials: F
authors.lastname: Mehrkhani
authors.forename: H
authors.initials: H
authors.lastname: Jamshidian
authors.forename: J
authors.initials: J
authors.lastname: Dastan
authors.forename: A R
authors.initials: AR
authors.lastname: Mosavi-Jarrahi
authors.forename: S R
authors.initials: SR
authors.lastname: Ghaffari
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000652:urine*; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D003581:Cytodiagnosis / Q000379:methods; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007492:Iran; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000652:urine*; D014556:Urine / Q000166:cytology*