Molecular cloning and characterization of porcine homeodomain transcription factor Msx1.
We cloned a porcine ortholog of homeodomain transcription factor Msx1 from the porcine pituitary cDNA library. The amino acid sequence of Msx1 shows high conservation among mammalian species. RT-PCR for porcine fetal and postnatal pituitaries showed that Msx1 is already expressed at early fetal day 40, decreases to a low level before birth and then remarkably decreases after birth. On the other hand, Msx1 expression was observed in all pituitary-derived cell line tested, with most in a gonadotrope lineage LbetaT4. Transfection assay demonstrated that Msx1 markedly repressed the basal Cga and Fshb gene expression, while Lhb expression was affected slightly. Taken together, Msx1 may play a role in repressing gene expression in the fetal and postnatal periods.
Ishikawa
Akio
A
Division of Life Science, Graduate School of Agriculture, Meiji University.
Kato
Takako
T
Susa
Takao
T
Sano
Akiko
A
Kato
Yukio
Y
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000235:genetics / Q000302:isolation & purification; D051957:MSX1 Transcription Factor / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000196:embryology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013552:Swine / Q000196:embryology / Q000235:genetics*
D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor