title: Effect of chitooligosaccharide on neuronal differentiation of PC-12 cells.
abstract: Chitosan is now being widely used biomaterial in the tissue engineering field, and has great potential as a candidate material for preparing nerve guidance conduits due to its various favorable properties, especially that of good nerve cell affinity. Chitosan can be degraded in vivo into chitooligosaccharide. We have investigated the in vitro effects of chitooligosaccharide on neuronal differentiation of PC-12 cells to see what effects chitooligosaccharide have on certain functions in the regenerating neurons. The morphologic observation and assessment using the specific reagent of tetrazolium salt WST-8 indicated that neurite outgrowths from PC-12 cells and the viability of PC-12 cells were enhanced by treatment of chitooligosaccharide. The real-time quantitative RT-PCR and Western blot analysis revealed showed that chitooligosaccharide could upregulate the expression of neurofilament-H mRNA or protein and N-cadherin protein in PC-12 cells. The maximum effect of 0.1 mg/ml chitooligosaccharide was obtained after 2 week culture. All the data suggest that chitooligosaccharide possesses good nerve cell affinity by supporting nerve cell adhesion and promoting neuronal differentiation and neurite outgrowth.
authors.affiliation: Nantong University, Jiangsu, PR China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yang
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.forename: Sheyu
authors.initials: S
authors.lastname: Lin
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D009844:Oligosaccharides / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D013778:Tetrazolium Salts / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C476329:2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt; D015820:Cadherins; D016900:Neurofilament Proteins; D009844:Oligosaccharides; D013778:Tetrazolium Salts; C051774:neurofilament protein H; D048271:Chitosan