A combination of molecular probe-tandem electrospray ionization mass spectrometry: a technique for tracing structural changes in phospholipid hydroperoxides.
An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.
Shimizu
Rumiko
R
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka, Okayama, Japan.
Nagai
Ai
A
Tominaga
Hiroko
H
Imura
Megumi
M
Onyango
Arnold N
AN
Izumi
Minoru
M
Nakajima
Shuhei
S
Tahara
Shoichi
S
Kaneko
Takao
T
Baba
Naomichi
N
D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D015335:Molecular Probes; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide