Effects of gonadotropin-exposed medium with high concentrations of progesterone and estradiol-17beta on in vitro maturation of canine oocytes.
During the process of maturation in the oviduct, canine oocytes in the germinal vesicle stage are exposed to decreasing levels of estradiol-17beta and increasing levels of progesterone. However, hormone concentrations in the microenvironments in which they act are higher than serum concentrations. Therefore, the aim of the present study was to compare the meiotic competence of canine oocytes harvested from anestrous bitches in culture medium containing high concentrations (20 microg ml(-1)) of estradiol-17beta and/or progesterone in association to gonadotropins (luteinizing hormone and follicle-stimulating hormone) using three different maturation periods (48, 72, and 96 h). Oocytes were cultured in tissue culture medium (TCM-199) and arranged in four experimental groups: group control, group E2 (estradiol-17beta), group P4 (progesterone), and group E2 + P4. Regardless of the maturation period, groups P4 and E2 + P4 presented statistically higher rate of germinal vesicle breakdown oocytes compared to the group control and group E2. There were no significant differences among groups on germinal vesicle, metaphase I, metaphase II, and degenerated or unidentifiable oocytes rates. The mean percentage of metaphase II oocytes was higher at 96 h when compared to 72 h. Results of the present research indicate no influence of estradiol-17beta supplementation, unless in association with progesterone. There is an evidence of the positive effect of progesterone on germinal vesicle breakdown. Results also showed that extended periods of in vitro maturation affect positively maturation rates to metaphase II of low competent oocytes harvested from anestrous bitches, independent of the maturation media. In conclusion, high concentrations of steroids, especially progesterone, have positive effect on in vitro oocyte maturation when the oocytes are derived from the anestrous status.
Vannucchi
Camila Infantosi
CI
Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, Cidade Universitária, São Paulo, São Paulo 05508-270, Brazil. cacavann@usp.br
Faustino
Marcelo
M
Marques
Mariana Groke
MG
Nichi
Marcílio
M
Assumpção
Mayra Elena Ortiz D'Avila
ME
Visintin
José Antonio
JA
D000818:Animals; D018929:Cell Culture Techniques; D048750:Cell Nucleus Division / Q000187:drug effects; D003470:Culture Media; D004285:Dogs; D004958:Estradiol / Q000494:pharmacology*; D006062:Gonadotropins / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D011374:Progesterone / Q000494:pharmacology*
D003470:Culture Media; D006062:Gonadotropins; D011374:Progesterone; D004958:Estradiol