title: [Association of polymorphisms of N372H in BRCA2 gene and 135G/C in RAD51 gene and breast cancers].
abstract: OBJECTIVE
  To determine the associations between the polymorphisms of N372H in BRCA2 gene and 135G/C in RAD51 gene and breast cancers.
  
  
  METHODS
  The allele and genotype frequencies of N372H in BRCA2 gene and 135G/C in RAD51 gene were analyzed with denaturing high performance liquid chromatography (DHPLC) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 71 women with breast cancers and 85 normal, women.
  
  
  RESULTS
  The women with breast cancers had higher frequencies of genotype HH of the N372H locus in BRCA2 gene than the normal women. No statistical difference in the polymorphic distribution of 135G/C in RAD51 gene was observed between the two groups of women.
  
  
  CONCLUSIONS
  The genotype HH of the N372H locus in BRCA2 is associated with breast cancers, which might be a genetic risk factor for breast cancers in Chinese populations.
authors.affiliation: Department of Mastologic Surgery, Sichuan Cancer Hospital, Sichuan Province, Chengdu 610041, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Hu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wei
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Jiang
authors.forename: Wen-xiu
authors.initials: WX
authors.lastname: Yao
authors.forename: Qi-ming
authors.initials: QM
authors.lastname: Long
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Tang
mesh_terms: D000368:Aged; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002681:China; D005260:Female; D024522:Genes, BRCA2; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051135:Rad51 Recombinase / Q000235:genetics*
chemical_list: C495153:RAD51 protein, human; D051135:Rad51 Recombinase