Evaluation of immunomodulatory activity of an extract of andrographolides from Andographis paniculata.
The immunomodulatory activity of HN-02, an extract containing a mixture of andrographolides (i.e., andrographolide [88 +/- 5 %] plus 14-deoxyandrographolide and 14-deoxy-11,12-didehydroandrographolide together [12 +/- 3 %]) in a pure powder form was evaluated at 1.0, 1.5, and 2.5 mg/kg on different in vivo and in vitro experimental models. In a delayed-type hypersensitivity (DTH) mouse model, potentiation of the DTH reaction was observed after treatment with cyclophosphamide (CYP) and HN-02 individually. However, CYP potentiation of the DTH reaction was reversed by HN-02 pretreatment. Furthermore, HN-02 treatment elevated the depressed hemagglutination antibody (HA) titer and increased the number of plaque-forming cells (PFCs) in the spleen cells of mice that had been treated with CYP and challenged with sheep red blood cells (SRBC). Further, it was also found that HN-02 treatment stimulated phagocytosis in mice. A significant increase in total WBC count and relative weight of spleen and thymus was observed in mice during 30 days of treatment with HN-02. The present experimental findings demonstrate that HN-02 has the ability to enhance immune function, possibly through modulation of immune responses altered during antigen interaction, and to reverse the immunosuppression induced by CYP.
Naik
Suresh R
SR
Prin. K. M. Kundnani College of Pharmacy, Rambhau Salgaonkar Marg, Cuff, Parade, Colaba, Mumbai, India. srnaik5@rediffmail.com
Hule
Amolkumar
A
D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D003520:Cyclophosphamide; D004195:Disease Models, Animal; D004224:Diterpenes / Q000494:pharmacology / Q000627:therapeutic use*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000188:drug therapy*; D007155:Immunologic Factors / Q000494:pharmacology / Q000627:therapeutic use*; D007166:Immunosuppressive Agents; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use*; D012756:Sheep; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
C495626:14-deoxy-11,12-didehydroandrographolide; C114791:14-deoxyandrographolide; D004224:Diterpenes; D007155:Immunologic Factors; D007166:Immunosuppressive Agents; D028321:Plant Preparations; C030419:andrographolide; D003520:Cyclophosphamide