title: In situ TEM and SEM studies on the antimicrobial activity and prevention of Candida albicans biofilm by Cassia spectabilis extract.
abstract: The inhibitory effect of Cassia spectabilis methanol leaf extract was evaluated against biofilm forming Candida albicans, which was sensitive to 6.25 mg/ml concentration of the extract. Transmission (TEM) and scanning electron microscope (SEM) observations were used to study the anticandidal activity and prevention of biofilm formation by the C. spectabilis extract. SEM analysis further revealed reduction in C. albicans biofilm in response to the extract. The main abnormalities noted via TEM study was the alterations in morphology and complete collapse of the yeast cells after 36 h of exposure to the extract. The significant antifungal activity shown by this methanol extract of C. spectabilis suggests its potential against infections caused by C. albicans.
authors.affiliation: School of Distance Education, Universiti Sains Malaysia, Minden, Penang, Malaysia.
authors.forename: S
authors.initials: S
authors.lastname: Sangetha
authors.forename: Z
authors.initials: Z
authors.lastname: Zuraini
authors.forename: S
authors.initials: S
authors.lastname: Suryani
authors.forename: S
authors.initials: S
authors.lastname: Sasidharan
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D002366:Cassia / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts