title: SK66-his, a novel glycine-rich peptide derived from Drosophila with antibacterial activity.
abstract: SK(66)-his, a novel glycine-rich peptide derived from the CG13551 gene of Drosophila, was directly expressed in Escherichia coli with the help of the glucose effect of the lac repressor and efficiently purified in high yield (10.063 mg/l). It showed significant activity against Gram-positive bacteria. We determined a MIC value of 9 microg ml(-1) with Bacillus thuringiensis.
authors.affiliation: Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Feng
authors.forename: Huijuan
authors.initials: H
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Lang
authors.forename: Yanjiao
authors.initials: Y
authors.lastname: Li
authors.forename: Muya
authors.initials: M
authors.lastname: Shu
authors.forename: Zhengwang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004330:Drosophila / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005998:Glycine; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D005998:Glycine