title: Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish.
abstract: During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.
authors.affiliation: Kyungpook National University, Buk-gu, Daegu, Republic of Korea.
authors.forename: Jun-Dae
authors.initials: JD
authors.lastname: Kim
authors.forename: Hang-Suk
authors.initials: HS
authors.lastname: Chun
authors.forename: Seok-Hyung
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Young-Seop
authors.initials: YS
authors.lastname: Kim
authors.forename: Myoung-Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jimann
authors.initials: J
authors.lastname: Shin
authors.forename: Myungchull
authors.initials: M
authors.lastname: Rhee
authors.forename: Sang-Yeob
authors.initials: SY
authors.lastname: Yeo
authors.forename: Tae-Lin
authors.initials: TL
authors.lastname: Huh
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D051157:Frizzled Receptors / Q000096:biosynthesis*; D018398:Homeodomain Proteins / Q000378:metabolism; D016548:Prosencephalon / Q000254:growth & development* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis*; D019170:Somites / Q000254:growth & development* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D051153:Wnt Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000096:biosynthesis*
chemical_list: C088334:Distal-less homeobox proteins; D051157:Frizzled Receptors; D018398:Homeodomain Proteins; D043562:Receptors, G-Protein-Coupled; D014157:Transcription Factors; D051153:Wnt Proteins; D029961:Zebrafish Proteins; C503354:fzd8a protein, zebrafish