Contribution of methylated exudate flavonoids to the anti-inflammatory activity of Grindelia robusta.
The flavonoid pattern of an acetonic extract of Grindelia robusta Nutt. was investigated in detail. The flavonoids were enriched by CC. In addition to twelve known methylated exudate flavonols four compounds were identified for the first time in G. robusta. Several substances of the flavonoid complex, among them the main compounds quercetin-3-methylether and 6-OH-kaempferol-3,6-dimethylether, were tested for their activity to inhibit neutrophil elastase. Quercetin-3-methylether was shown to be most active with an IC(50) of 19 microM, thus obviously contributing to the anti-inflammatory activity of the drug.
Krenn
Liselotte
L
Department of Pharmacognosy, University of Vienna, PharmaCenter Vienna, Vienna, Austria. liselotte.krenn@univie.ac.at
Wollenweber
Eckhard
E
Steyrleuthner
Katja
K
Görick
Cornelia
C
Melzig
Matthias F
MF
D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D044948:Flavonols / Q000302:isolation & purification* / Q000494:pharmacology; D036623:Grindelia / Q000737:chemistry*; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives
C543233:6-OH-kaempferol-3,6-dimethylether; D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D044948:Flavonols; D010936:Plant Extracts; C459210:quercetin 3-O-methyl ether; D011794:Quercetin; D019272:Leukocyte Elastase