title: [Beneficial effect of total flavonoids of Chrysanthemum indicum on adjuvant arthritis by induction of apoptosis of synovial fibroblasts].
abstract: OBJECTIVE
  To investigate the effect on the extract of total flavonoids of Chrysanthemum indicum (TFC) on adjuvant arthritis synovial cells.
  
  
  METHODS
  SD rats were divided randomly into six groups including normal, model, TFC (84, 168, 336 mg x kg(-1)) and control drug Tripterygium glycosides (30 mg x kg(-1)) groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0.1 mL of the complete Freund's adjuvant into the right hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MT; The apoptosis rates of synovial cells were evaluated using TUNEL and FCM analysis.
  
  
  RESULTS
  TFC resulted in a dose-dependent way in inhibiting the proliferation of synovial and inducing the apoptosis of synovium and synoviocytes in vivo.
  
  
  CONCLUSIONS
  TFC can inhibit proliferation and induce apoptosis in synovial cells, and exert therapeutical effect on rheumatoid arthritis.
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei 230032, China. xuefengtt@yahoo.com.cn
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.forename: Cheng-mu
authors.initials: CM
authors.lastname: Hu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D049109:Cell Proliferation / Q000187:drug effects; D031202:Chrysanthemum / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005419:Flavonoids / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids