title: Compatibility of hyaluronic acid hydrogel and skeletal muscle myoblasts.
abstract: Compatibility of hyaluronic acid hydrogel (HAH) and skeletal muscle myoblasts has been investigated for the first time in the present paper. Skeletal muscle myoblasts were separated from skeletons of rats and incubated with a HAH-containing culture medium. Cell morphology, hydrophilicity and cell adhesion of the HAH scaffold were investigated using optical microscopy, scanning electron microscopy, Hoechest33258 fluorescent staining, the immunocytochemistry method and water adsorption rate measurement. It was found that at a proper concentration (around 0.5%) of hyaluronic acid, the hydrogel possessed good compatibility with skeletal muscle myoblasts. The hydrogel can create a three-dimensional structure for the growth of skeletal muscle myoblasts and benefit cell attachment to provide a novel scaffold material for the tissue engineering of skeletal muscle.
authors.affiliation: Jinzhou Central Hospital, Jinzhou 121000, People's Republic of China. Weiwang Ly@yahoo.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Shu-hong
authors.initials: SH
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.forename: Cheng-yue
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D006690:Bisbenzimidazole / Q000494:pharmacology; D049109:Cell Proliferation; D003470:Culture Media / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology*; D020100:Hydrogels / Q000737:chemistry*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects; D032446:Myoblasts / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D005456:Fluorescent Dyes; D020100:Hydrogels; D006820:Hyaluronic Acid; D006690:Bisbenzimidazole