Screening of SMG7-binding peptides by combination of phage display and docking simulation analysis.
We screened SMG7-binding peptides with phage display and docking simulation analysis. Although a consensus motif was absent in the phage display-derived candidates, we succeeded to find a peptide CDDRPPKSC, which can bind specifically to SMG7. We conclude that docking simulation helps to find high-affinity peptides efficiently, even if the phage display-screened candidates lack a consensus region.
Zahed
Muhammed
M
Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Inohana, Chiba 260-8670, Japan.
Suzuki
Toshikazu
T
Suganami
Akiko
A
Sugiyama
Hajime
H
Harada
Kazuo
K
Takiguchi
Masaki
M
Tamura
Yutaka
Y
Suzuki
Nobuo
N
D002352:Carrier Proteins / Q000378:metabolism*; D003198:Computer Simulation; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding
D002352:Carrier Proteins; D019151:Peptide Library; D010455:Peptides; C494578:SMG7 protein, human