An in vitro characterization study of new near infrared dyes for molecular imaging.
The spectroscopic properties, stability, and cytotoxicity of series of cyanine labels, the dyes DY-681, DY-731, DY-751, and DY-776, were studied to identify new tools for in vivo fluorescence imaging and to find substitutes for DY-676 recently used by us as fluorescent label in a target-specific probe directed against carcinoembryonic antigen (CEA). This probe enables the selective monitoring of CEA-expressing tumor cells in mice, yet displays only a low fluorescence quantum yield and thus, a non-optimum sensitivity. All the DY dyes revealed enhanced fluorescence quantum yields, a superior stability, and a lower cytotoxicity in comparison to clinically approved indocyanine green (ICG). With DY-681 and far-red excitable DY-731 and DY-751, we identified three dyes with improved properties compared to DY-676 and ICG.
Pauli
Jutta
J
BAM Bundesanstalt für Materialforschung und -prüfung, OE I.5, Richard-Willstaetter-Str. 11, D-12489 Berlin, Germany.
Vag
Tibor
T
Haag
Romy
R
Spieles
Monika
M
Wenzel
Matthias
M
Kaiser
Werner A
WA
Resch-Genger
Ute
U
Hilger
Ingrid
I
D000475:Alkenes / Q000737:chemistry* / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000633:toxicity*; D007211:Indoles / Q000737:chemistry* / Q000633:toxicity*; D008264:Macrophages / Q000166:cytology; D015394:Molecular Structure; D051189:Murinae; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000475:Alkenes; D005456:Fluorescent Dyes; D007211:Indoles