[Changes of some elements in rat's tissues except nerve centre with both ovariectomy and chronic aluminum toxication and the effects of estrogen supplement].
OBJECTIVE
To reveal the influence of ovariectomy and chronic Aluminum toxication in combination on the distribution in tissues except nerve centre and excretion in urine of somel elements. The study will supply the basis for study of Alzheimers disease.


METHODS
Forty 6-month-old female S-D rats were divided randomly into 4 groups: group OVX, group OVX + Al and group OVX + Al + E2. After 3 months, urine of 24 h were collected by metabolic cages; liver, kidney, heart, bone, tibia and skeletal muscle, were got from the rats. The content of the elements in these tissues and urine was detected by ICP-AES.


RESULTS
Compared of group OVX with group sham, the content of Zn decreased (P < 0.001). Compared of group OVX + Al + E2 with group sham, the content of Al, Cd, Si and Se in heart increased (P < 0.05, P < 0.01, P < 0.01, P < 0.001). The content of Se and Cd in tibia increased (P < 0.01, P < 0.001). The content of Al in kidney increased (P < 0.05). The content of Mn and Cu increased (P < 0.05, P < 0.001), Se decreased (P < 0.001) in skeletal muscle. The content of Al, Se and Ca in spinal cord decreased (P < 0.05, P < 0.01, P < 0.05). The content of Mn, Zn and Si in liver increased (P < 0.01, P < 0.05, P < 0.05). The content of Cd, Mg, Se, Al and Ca in urine increased (P < 0.05, P < 0.05, P < 0.01, P < 0.001, P < 0.001). Compared of group OVX + Al + E2 with group OVX + Al, the content of Cd, Mn and Se in heart increased (P < 0.05, P < 0.05, P < 0.01). The content of Al, Mg, Se, Cd and Mn in tibia increased (P < 0.05, P < 0.01, P < 0.001, P < 0.001, P < 0.001). The content of Mn and Cu increased (P < 0.05, P < 0.001), Se decreased (P < 0.001), in skeletal muscle. The content of Se in spinal cord decreased (P < 0.05). The content of Al and Ca decreased (P < 0.05, P < 0.01), the content of Cu, Si, Fe, Mg, Mn and Zn incraesed(P <0.05, P < 0.05, P <0.01, P < 0.01, P < 0.001, P < 0.001), in liver. The content of Se, Al, Cd, Mg, Si and Ca in urine increased (P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.01).


CONCLUSIONS
Zn in heart can transfer to other tissues after a long time ovariectomy. Heart, skeletal muscle and liver, are mainly affected tissues except nerve centre by ovariectomy and chronic aluminum toxication in combination; and mainly affected elements are Zn, Si, Cu and Se. Chronic aluminum toxication make Si transfer to heart of ovariectomized rats, and facilitate Zn in heart transfer to other tissues. Supply estrogen can promote aluminum excretion in urine. distribution in tissues except nerve centre and excretion in urine of somel elements. The study will supply the basis for study of Alzheimers disease.


METHODS
Forty 6-month-old female S-D rats were divided randomly into 4 groups: group OVX, group OVX + Al and group OVX + Al + E2. After 3 months, urine of 24h were collected by metabolic cages; Liver, kidney, heart, bone, tibia and skeletal muscle, were got from the rats. The content of the elements in these tissues and urine was detected by ICP-AES.


RESULTS
Compared of group OVX with group sham, the content of Zn decreased (P < 0.001). Compared of group OVX + Al + E2 with group sham, the content of Al, Cd, Si and Se in heart increased (P < 0.05, P < 0.01, P < 0.01, P < 0.001). The content of Se and Cd in tibia increased (P < 0.01, P < 0.001). The content of Al in kidney increased (P < 0.05). The content of Mn and Cu increased (P < 0.05, P < 0.001),Se decreased (P < 0.001) in skeletal muscle. The content of Al, Se and Ca in spinal cord decreased (P < 0.05, P < 0.01, P < 0.05). The content of Mn, Zn and Si in liver increased (P < 0.01, P < 0.05, P < 0.05). The content of Cd, Mg, Se, Al and Ca in urine increased (P < 0.05, P < 0.05, P < 0.01, P < 0.001, P < 0.001). Compared of group OVX + Al + E2 with group OVX + Al, the content of Cd, Mn and Se in heart increased (P < 0.05, P < 0.05, P < 0.01). The content of Al, Mg, Se, Cd and Mn in tibia increased (P < 0.05, P < 0.01, P < 0.001, P < 0.001, P < 0.001). The content of Mn and Cu increased (P < 0.05, P < 0.001), Se decreased (P < 0.001), in skeletal muscle. The content of Se in spinal cord decreased (P < 0.05). The content of Al and Ca decreased (P < 0.05, P < 0.01), the content of Cu, Si, Fe, Mg, Mn and Zn incraesed(P <0.05, P < 0.05, P <0.01, P < 0.01, P < 0.001, P < 0.001), in liver. The content of Se, Al, Cd, Mg, Si and Ca in urine increased (P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.01).


CONCLUSIONS
Zn in heart can transfer to other tissues after a long time ovariectomy. Heart, skeletal muscle and liver, are mainly affected tissues except nerve centre by ovariectomy and chronic aluminum toxication in combination; and mainly affected elements are Zn, Si, Cu and Se. Chronic aluminum toxication make Si transfer to heart of ovariectomized rats, and facilitate Zn in heart transfer to other tissues. Supply estrogen can promote aluminum excretion in urine.
Zhao
Changan
C
Department of Biochemistry and Molecular Biology of Xinxiang Medical University, Xinxiang 453003, China. zca_phd@yahoo.com.cn
Xu
Na
N
Zhang
Wen
W
Zhao
Chunpeng
C
D000535:Aluminum / Q000633:toxicity* / Q000652:urine; D000544:Alzheimer Disease / Q000209:etiology; D000818:Animals; D003300:Copper / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D004967:Estrogens / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D010052:Ovariectomy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
D004785:Environmental Pollutants; D004967:Estrogens; D003300:Copper; D000535:Aluminum; D012643:Selenium; D015032:Zinc