ThPOD3, a truncated polypeptide from Tamarix hispida, conferred drought tolerance in Escherichia coli.
The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS-PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were approximately 57, approximately 50 and approximately 47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.
Guo
Xiao-Hong
XH
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin, China.
Jiang
Jing
J
Wang
Bai-Chen
BC
Li
Hui-Yu
HY
Wang
Yu-Cheng
YC
Yang
Chuan-Ping
CP
Liu
Gui-Feng
GF
D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D055864:Droughts; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D009195:Peroxidase / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000502:physiology*; D016019:Survival Analysis; D032363:Tamaricaceae / Q000235:genetics*
D017931:DNA Primers; D009195:Peroxidase