title: Study on the enhanced cellular uptake effect of daunorubicin on leukemia cells mediated via functionalized nickel nanoparticles.
abstract: The success of cancer chemotherapy is largely dependent on the efficient anticancer drug accumulation in target tumor tissues and cells so as to inhibit the proliferation of the cancer cells. Recently, some biocompatible nanomaterials have been utilized as drug target delivery systems and have shown the great potential to effectively afford the sustained drug delivery for the target cancer cells. In this study, we have explored the possibility for the bio-application of the functionalized nickel (Ni) nanoparticles and the efficiency of the functionalized Ni nanoparticles on drug permeability, and cellular uptake of leukemia K562 cells in vitro has been probed via atomic force microscopy, inverted fluorescence microscopy and confocal microscopy, electrochemical study and MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl-tetrazolium bromide) assay. It is observed that the presence of relevant Ni nanoparticles could induce the membrane structure change of target cells and efficiently improve the permeability of the cell membrane so that the combination of these Ni nanoparticles with anticancer drug daunorubicin could have a synergistic effect on the efficient cytotoxicity suppression in leukemia cancer cells. These observations indicate the great potential of Ni nanoparticles in the future biomedical application including target cancer diagnosis and chemotherapy.
authors.affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Southeast University, Nanjing 210096, People's Republic of China.
authors.forename: Dadong
authors.initials: D
authors.lastname: Guo
authors.forename: Chunhui
authors.initials: C
authors.lastname: Wu
authors.forename: Hongli
authors.initials: H
authors.lastname: Hu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Li
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000737:chemistry; D045744:Cell Line, Tumor; D003630:Daunorubicin / Q000008:administration & dosage*; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D009532:Nickel / Q000737:chemistry*; D010539:Permeability; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; D009532:Nickel; C022616:thiazolyl blue; D003630:Daunorubicin