title: [Biochemical methods for the analysis of DNA-protein interactions].
abstract: Investigation of DNA-protein interactions is fundamental to understand the mechanism underlying a variety of life processes. In this article, various types of biochemical methods in DNA-protein interaction study in vivo and in vitro at the level of DNA, protein, and the complex, respectively were briefly reviewed. Traditional assays including Nitrocellulose filter-binding assay, Footprinting, EMSA, and Southwestern blotting were summarized. In addition, chromatin immunoprecipitation techniques including nChIP, xChIP, and ChIP-on-chip, which were widely used in epigenetics, were particularly introduced.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. 2007105010@njau.edu.cn
authors.forename: Jin-Bi
authors.initials: JB
authors.lastname: Zhang
authors.forename: Zeng-Xiang
authors.initials: ZX
authors.lastname: Pan
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Ma
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Liu
mesh_terms: D015139:Blotting, Southern; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D008957:Models, Genetic; D011485:Protein Binding; D023302:Protein Footprinting; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins; D004247:DNA