title: Platelet leukocyte gel facilitates bone substitute growth and autologous bone growth in a goat model.
abstract: The aim of this study is to evaluate multiple conditions on the formation of bone growth in a goat model. We prepared from a unit of whole blood, platelet-leukocyte gel (PLG) to stimulate bone formation, based on the release of platelet growth factors. Two 3-compartment cages containing autologous bone, calcium phosphate, and trabecular metal were implanted onto goat spinal transverse processes. One cage was treated with PLG, prepared according to a standardized protocol. An untreated cage served as a control. To monitor bone formation overtime, fluorochrome markers were administered at 2, 3, and 5 weeks. Animals were sacrificed at 9 weeks after implantation. Bone growth in these 3-compartments cages was examined by histology and histomorphometry of nondecalcified sections using traditional light and epifluorescent microscopy. Compared to the control samples, bone growth in the PLG-treated autologous bone and calcium phosphate samples was significantly more. Fairly little bone growth was seen in PLG treated or untreated trabecular metal scaffolds. The results obtained from this goat model suggest a potential role for the application of autologous PLG during surgeries in which autologous bone grafts or calcium phosphate scaffolds are used.
authors.affiliation: Department of Peri-Operative Blood Management, Catharina Hospital, Eindhoven, The Netherlands. everts@elive.nl
authors.forename: Peter A M
authors.initials: PA
authors.lastname: Everts
authors.forename: Diyar
authors.initials: D
authors.lastname: Delawi
authors.forename: Christine Brown
authors.initials: CB
authors.lastname: Mahoney
authors.forename: Albert
authors.initials: A
authors.lastname: van Erp
authors.forename: Eddy P
authors.initials: EP
authors.lastname: Overdevest
authors.forename: André
authors.initials: A
authors.lastname: van Zundert
authors.forename: Johannes T A
authors.initials: JT
authors.lastname: Knape
authors.forename: Wouter J A
authors.initials: WJ
authors.lastname: Dhert
mesh_terms: D000758:Anesthesia; D000818:Animals; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000737:chemistry*; D001846:Bone Development / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry*; D015194:Diffusion Chambers, Culture; D005260:Female; D005456:Fluorescent Dyes; D005782:Gels; D006041:Goats / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D007962:Leukocytes / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D019736:Prostheses and Implants; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D005456:Fluorescent Dyes; D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins; D053773:Transforming Growth Factor beta1