title: Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors.
abstract: Glutamate receptors of the AMPA-subtype (AMPARs), together with the transmembrane AMPAR regulatory proteins (TARPs), mediate fast excitatory synaptic transmission in the mammalian brain. Here, we show by proteomic analysis that the majority of AMPARs in the rat brain are coassembled with two members of the cornichon family of transmembrane proteins, rather than with the TARPs. Coassembly with cornichon homologs 2 and 3 affects AMPARs in two ways: Cornichons increase surface expression of AMPARs, and they alter channel gating by markedly slowing deactivation and desensitization kinetics. These results demonstrate that cornichons are intrinsic auxiliary subunits of native AMPARs and provide previously unknown molecular determinants for glutamatergic neurotransmission in the central nervous system.
authors.affiliation: Institute of Physiology II, University of Freiburg, Engesserstrasse 4, 79108 Freiburg, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Schwenk
authors.forename: Nadine
authors.initials: N
authors.lastname: Harmel
authors.forename: Gerd
authors.initials: G
authors.lastname: Zolles
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bildl
authors.forename: Akos
authors.initials: A
authors.lastname: Kulik
authors.forename: Bernd
authors.initials: B
authors.lastname: Heimrich
authors.forename: Osamu
authors.initials: O
authors.lastname: Chisaka
authors.forename: Peter
authors.initials: P
authors.lastname: Jonas
authors.forename: Uwe
authors.initials: U
authors.lastname: Schulte
authors.forename: Bernd
authors.initials: B
authors.lastname: Fakler
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Klöcker
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D015640:Ion Channel Gating; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D018091:Receptors, AMPA / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism; D009435:Synaptic Transmission; D014981:Xenopus; D009419:Nerve Tissue Proteins
chemical_list: D018698:Glutamic Acid; D008565:Membrane Proteins; D021122:Protein Subunits; D018091:Receptors, AMPA; C539313:Cnih2 protein, rat; D009419:Nerve Tissue Proteins; C539314:Cnih3 protein, rat