Characterization of the murine Ephrin-B2 promoter.
Eph/ephrin signaling is pivotal in prenatal angiogenesis. Although their role in these processes has been extensively studied the mechanisms controlling eph/ephrin expression is not fully understood. To better understand the transcriptional regulation of arteriogenesis we have cloned and characterized the promoter of ephrin-B2 and its regulatory elements. The transcription start site was identified using 5' RACE and through deletion studies a minimal promoter region was determined. This 180 bp region located just upstream of the transcription start site has a high GC content and contains a consensus TATA-box which proved to be vital for basal transcriptional activity. In addition, putative regulatory elements for five transcription factors were identified. Mutational destruction of individual binding sites and chromatin immunoprecipitation confirmed that Meis1, MAZ and NFY physically interacted with the region and regulated promoter activity.
Sohl
Marcus
M
Department of Cell and Molecular Biology, Karolinska Institutet, Box 285, SE 171 77, Stockholm, Sweden. marcus.sohl@ki.se
Lanner
Fredrik
F
Farnebo
Filip
F
D000818:Animals; D001158:Arteries / Q000166:cytology; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D036388:Ephrin-B2 / Q000235:genetics*; D018398:Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism
D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins; D036388:Ephrin-B2; D018398:Homeodomain Proteins; C000616392:Meis1 protein, mouse; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins; D014157:Transcription Factors; C076035:c-MYC-associated zinc finger protein; C402244:nuclear factor Y