[Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector].
To construct a drug carrier and gene vector PEG-PEI-Pt.



Polyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells.



The structure of PEG-PEI-Pt was characterized by XRD, UV-VIS and FT-IR. PEG-PEI-Pt complex was able to bind DNA at N/P weight ratio of 0.4:1; the complex showed cytotoxicity on Hela and B16 cells. The complex had higher transfection efficiency in A293 and B16 cells than PEI 600.



A novel drug carrier and gene vector PEG-PEI-Pt was constructed successfully.
Zhao
Lu
L
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
Chen
Huan
H
Chen
Dan
D
Hu
Yi-Ping
YP
Yu
Hai
H
Wang
Qing-Qing
QQ
Tang
Gu-Ping
GP
Hu
Xiu-Rong
XR
D002460:Cell Line; D004337:Drug Carriers / Q000138:chemical synthesis*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D017671:Platinum Compounds / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection
D004337:Drug Carriers; D017671:Platinum Compounds; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; C032741:platinum tetrachloride