Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors.
Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO(2) waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.
Gopinath
Subash C B
SC
Center for Applied Near Field Optics Research (CAN-FOR), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
Awazu
Koichi
K
Fujimaki
Makoto
M
Sugimoto
Katsuaki
K
Ohki
Yoshimichi
Y
Komatsubara
Tetsuro
T
Tominaga
Junji
J
Kumar
Penmetcha K R
PK
D015374:Biosensing Techniques / Q000379:methods*; D049329:Nanostructures / Q000032:analysis* / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D012822:Silicon Dioxide