title: Autoantibody to NA14 is an independent marker primarily for Sjogren's syndrome.
abstract: Nuclear Autoantigen of 14 kDa (NA14) was originally identified using the serum of a Sjögren's syndrome (SS) patient as probe in screening a human testis cDNA expression library. To date there is no report in the systematic analysis of the prevalence of autoantibodies to NA14. In this study, anti-NA14 was determined in several rheumatic diseases from independent cohorts in the US and Japan. The prevalence of anti-NA14 were 18/132 (13.6%) in primary SS, 0/50 (0%) secondary SS, 2/100 (2%) SLE, 1/43 (2.3%) scleroderma, 0/54 (0%) rheumatoid arthritis, 1/29 (3.4%) polymyositis/dermatomyositis, and 0/58 (0%) normal healthy controls. The frequencies of anti-NA14 positive sera in primary SS are statistically greater than normal healthy controls (p=0.006), secondary SS (p=0.044), and other rheumatic diseases. Furthermore, among 11 anti-NA14 positive primary SS sera, 4/11 (36.3%) sera were negative for both anti-SS-A/Ro and SS-B/La antibodies. Thus anti-NA14 autoantibodies may be useful for the discrimination of primary versus secondary SS and serve as a diagnostic marker for primary SS especially in seronegative (anti-SS-A/Ro and anti-SS-B/La antibodies negative) patients with SS.
authors.affiliation: Department of Rheumatology and Internal Medicine, Juntendo University Urayasu hospital, Institute for Environment and Gender Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nozawa
authors.forename: Keigo
authors.initials: K
authors.lastname: Ikeda
authors.forename: Minoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Westley H
authors.initials: WH
authors.lastname: Reeves
authors.forename: Carol M
authors.initials: CM
authors.lastname: Stewart
authors.forename: Yueh-Chun
authors.initials: YC
authors.lastname: Li
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Yen
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Rios
authors.forename: Kenji
authors.initials: K
authors.lastname: Takamori
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Iwao
authors.initials: I
authors.lastname: Sekigawa
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Takasaki
authors.forename: Edward K L
authors.initials: EK
authors.lastname: Chan
mesh_terms: D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015415:Biomarkers / Q000097:blood*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009687:Nuclear Proteins / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; C110204:NA14 nuclear autoantigen; D009687:Nuclear Proteins