[Effects of PUVA on mitochondrial membrane potential in HL-60 and K562 cells].
OBJECTIVE
To study the effects of psoralen (PSO) and long wave ultraviolet light (PUVA) on mitochondrial membrane potential in HL-60 and K562 cells.


METHODS
Cells were incubated with PSO in different concentrations irradiated with or without UVA. The changes of ultrastructure of cells were observed under the electron microscope. The apoptosis ratios and the changes of mitochondrial membrane potential were detected through the flow cytometry. The factorial design and analysis of variance were used to analyze the interaction among the factors.


RESULTS
There were obvious ultrastructure changes about apoptosis in HL-60 and K562 cells after treated with PUVA. PSO, UVA and PUVA all increased the apoptosis ratios and decreased the mitochondrial membrane potential, and the effects of PUVA were stronger than the other two (p<0.01).


CONCLUSIONS
PUVA can induce the apoptosis of HL-60 and K562 cells and one of the pathway to induce apoptosis is to downregulate the mitochondrial membrane potential.
Chen
Nan-Nan
NN
Department of Traditional Chinese Medicine and Hematology, 210th Hospital of PLA, Dalian 116021, China. chnngzx@163.com
Liu
Chun-Yu
CY
Huang
Shi-Lin
SL
Xiang
Yang
Y
Zhang
Chen
C
Zhang
De-Jie
DJ
D017209:Apoptosis / Q000187:drug effects*; D015536:Down-Regulation; D005434:Flow Cytometry; D011564:Furocoumarins / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects* / Q000502:physiology; D011701:PUVA Therapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
D011564:Furocoumarins; D017319:Photosensitizing Agents