Microplate hybridization assay for detection of isoniazid resistance in Mycobacterium tuberculosis.
Early and accurate detection of drug resistant Mycobacterium tuberculosis can improve both the treatment outcome and public health control of tuberculosis. A number of molecular-based techniques have been developed including ones using probe molecules that target drug resistance-related mutations. Although these techniques are highly specific and sensitive, mixed signals can be obtained when the drug resistant isolates are mixed with drug susceptible isolates. In order to overcome this problem, we developed a new drug susceptibility test (DST) for one of the most effective anti-tuberculosis drug, isoniazid. This technique employed a microplate hybridization assay that quantified signals from each probe molecule, and was evaluated using clinical isolates. The evaluation analysis clearly showed that the microplate hybridization assay was an accurate and rapid method that overcame the limitations of DST based on conventional molecular techniques.
Han
Hye Eun
HE
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 220-710, Korea.
Lee
In Soo
IS
Hwang
Joo Hwan
JH
Bang
Hye Eun
HE
Kim
Yeun
Y
Cho
Sang-Nae
SN
Kim
Tae Ue
TU
Lee
Hye Young
HY
D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology* / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy / Q000382:microbiology
D000995:Antitubercular Agents; D007538:Isoniazid