title: Immobilization of albumin on aminosilane modified superparamagnetic magnetite nanoparticles and its characterization.
abstract: Magnetite nanoparticles (NPs) were synthesized by co-precipitating Fe(2+) and Fe(3+) in an ammonia solution. Magnetite NPs coated with 3-aminopropyltriethoxysilane (APTES) were prepared by silanization reaction. After activated with glutaraldehyde (GA), human serum albumin was covalently immobilized on these magnetic NPs. The influence of three different buffer solutions on covalent immobilization was studied. phosphate buffer saline solution (PBS) was shown the highest binding ability for immobilization process compared to other buffer solutions. The morphology and properties of these magnetic NPs were examined by transmission electron eicroscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA) and magnetization measurement.
authors.affiliation: Department of Chemistry, Selcuk University, Konya 42075, Turkey.
authors.forename: Keziban
authors.initials: K
authors.lastname: Can
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozmen
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ersoz
mesh_terms: D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011437:Propylamines; D012709:Serum Albumin / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011437:Propylamines; D012709:Serum Albumin; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D052203:Ferrosoferric Oxide