[The effect of ischemic postconditioning on apoptosis induced by acute hot renal ischemic-reperfusion].
OBJECTIVE
To investigate the effects of ischemic postconditioning(IPo) on cellular apoptosis in acute renal ischemic-reperfusion injury (IRI) in rats.


METHODS
Model of acute renal ischemic-reperfusion injury (IRI) was constructed using Wistar rats. Forty healthy male rats were divided into 4 groups randomly,including group C,group IRI, group IPo and group Ado. Rats of group C received right nephronectomy and separation of left renal vessels. Before reperfusion, group IPo was perfused for 10 s, following ischemic reperfusion for another 10 s with 6 cycles. Group Ado was infused with adenosine intravenously 10 min before ischemia. Cellular apoptosis was determined with TUNEL (TdT-mediated dUTP Nick End Labelling), flowcytometry and histopathological staining.


RESULTS
Compared with group C, the apoptotic index increased predominatly in group IRI (P<0.05), whereas, apoptosis index decreased obviousely in group IPo, Ado (P<0.05). In addition, the apoptosis index between group IPo and Ado was not statistically different.


CONCLUSIONS
Ischemic postconditioning could decrease cellular apoptosis induced by IRI in rat kidney.
Zhu
Yu-chun
YC
Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Tang
Tie-long
TL
Cui
Shu
S
Zhou
Bin
B
Wang
Wei
W
Zeng
Guo-jun
GJ
Lu
Yi-ping
YP
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply* / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*