Extracellular chitinases of mutant superproducing strain Serratia marcescens M-1.
Four extracellular proteins with chitinase activity capable of binding chitin substrates have been revealed in the culture liquid of chitinase superproducing mutant strain M-1 of Serratia marcescens. Proteins were analyzed by SDS-PAGE and MALDI-TOF mass spectrometry. Based on the data obtained, the proteins were identified as typical chitinases of S. marcescens: ChiA, ChiB, ChiC, and CBP21.
Duzhak
A B
AB
Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. abduzhak@mail.ru
Panfilova
Z I
ZI
Duzhak
T G
TG
Vasyunina
E A
EA
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000201:enzymology; D011485:Protein Binding; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D003470:Culture Media; D002686:Chitin; D002688:Chitinases