Structural identification and cytotoxic activity of a polysaccharide from the fruits of Lagenaria siceraria (Lau).
A water-soluble polysaccharide, isolated from fruiting bodies of Lagenaria siceraria, is composed of methyl-alpha-d-galacturonate, 3-O-acetyl methyl-alpha-d-galacturonate, and beta-d-galactose in a ratio of nearly 1:1:1. Compositional analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, 2D-COSY, TOCSY, NOESY, HMQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide: [carbohydrate structure: see text] This polysaccharide showed cytotoxic activity in vitro against human breast adenocarcinoma cell line (MCF-7).
Ghosh
Kaushik
K
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Chandra
Krishnendu
K
Ojha
Arnab K
AK
Sarkar
Siddik
S
Islam
Syed S
SS
D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019662:Cucurbitaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D011134:Polysaccharides