Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats.
OBJECTIVE
Hydrogen has been reported to selectively reduce the hydroxyl radical, the most cytotoxic of reactive oxygen species. In this study we investigated the effects of hydrogen-rich saline on the prevention of lung injury induced by intestinal ischemia/reperfusion (I/R) in rats.


METHODS
Male Sprague-Dawley rats (n=30, 200-220g) were divided randomly into three experimental groups: sham operated, intestinal I/R plus saline treatment (5ml/kg, i.v.), and intestinal I/R plus hydrogen-rich saline treatment (5ml/kg, i.v.) groups. Intestinal I/R was produced by 90min of intestinal ischemia followed by a 4h of reperfusion.


RESULTS
Hydrogen-rich saline treatment decreased the neutrophil infiltration, the lipid membrane peroxidation, NF-kappaB activation and the pro-inflammatory cytokine interleukin IL-1beta and TNF-alpha in the lung tissues compared with those in saline-treated rat.


CONCLUSIONS
Hydrogen-rich saline attenuates lung injury induced by intestinal I/R.
Mao
Yan-Fei
YF
Department of Surgical Intensive Care Unit, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, PR China.
Zheng
Xing-Feng
XF
Cai
Jian-Mei
JM
You
Xin-Min
XM
Deng
Xiao-Ming
XM
Zhang
John H
JH
Jiang
Lai
L
Sun
Xue-Jun
XJ
D000818:Animals; D002462:Cell Membrane; D006859:Hydrogen / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000378:metabolism; D007422:Intestines / Q000098:blood supply; D015227:Lipid Peroxidation; D055370:Lung Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications; D012965:Sodium Chloride / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D006859:Hydrogen