Proteomic analysis of proteins bound to adsorption units of extracorporeal liver support system under clinical conditions.
Fractionated Plasma Separation, Adsorption and Dialysis (Prometheus) has a well-documented capacity to remove protein-bound organic toxins in patients with liver failure. However, the compositions of adsorbed proteins remain unknown. Elution of both adsorbers constituting Prometheus system was performed following a 6-h session in a patient with acute on chronic liver failure. Sodium dodecylsulphate was employed to elute proteins from the neutral adsorber (P1), while acetic acid was applied to the cationic one (P2). Eluted proteins were resolved by two-dimensional gel electrophoresis (2-DE) and identified by mass spectrometry (MS). Totally, 4113 and 8280 mg of proteins were obtained from P1 and P2 eluates, 2-DE yielded 148 and 163 protein fractions in P1 and P2, respectively. MS identified 18 unique proteins in P1, and 30 unique proteins in P2 sample. Proteins with the highest selective adsorption (as determined by eluate to plasma ratio) included transthyretin (37), trypsin-2 (29), prothrombin (23), hyaluronan-binding protein 2 (13) and plasma retinol-binding protein (8.7), all of which adsorbed to P2. We identified a large number of proteins removed by extracorporeal liver support system. A selective adsorption was demonstrated in a subset of proteins depending on the type of adsorber and proteins' characteristics.
Mares
Jan
J
Department of Internal Medicine I, Charles University Medical School and Teaching Hospital, Plzen, Czech Republic. mares@fnplzen.cz
Thongboonkerd
Visith
V
Tuma
Zdenek
Z
Moravec
Jiri
J
Karvunidis
Thomas
T
Matejovic
Martin
M
D000327:Adsorption; D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D017093:Liver Failure / Q000378:metabolism*; D019164:Liver, Artificial; D008297:Male; D020543:Proteome / Q000378:metabolism*; D016060:Sorption Detoxification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome