CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation.
CX(3)CR1 expression is associated with the commitment of CSF-1R(+) myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R(+) CX(3)CR1(+) macrophage/DC precursor (MDP) with other DC precursors and the role of CX(3)CR1 in macrophage and DC development remain unclear. We show that MDPs give rise to conventional DCs (cDCs), plasmacytoid DCs (PDCs), and monocytes, including Gr1(+) inflammatory monocytes that differentiate into TipDCs during infection. CX(3)CR1 deficiency selectively impairs the recruitment of blood Gr1(+) monocytes in the spleen after transfer and during acute Listeria monocytogenes infection but does not affect the development of monocytes, cDCs, and PDCs.
Auffray
Cedric
C
Laboratory of Biology of the Mononuclear Phagocyte System, Institut National de la Santé et de la Recherche Médicale U838, Université Paris-Descartes, 75015 Paris, France.
Fogg
Darin K
DK
Narni-Mancinelli
Emilie
E
Senechal
Brigitte
B
Trouillet
Celine
C
Saederup
Noah
N
Leemput
Julia
J
Bigot
Karine
K
Campisi
Laura
L
Abitbol
Marc
M
Molina
Thierry
T
Charo
Israel
I
Hume
David A
DA
Cumano
Ana
A
Lauvau
Gregoire
G
Geissmann
Frederic
F
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D000073883:CX3C Chemokine Receptor 1; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D003713:Dendritic Cells / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D007249:Inflammation / Q000201:enzymology* / Q000276:immunology; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000172:deficiency / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D051941:fms-Like Tyrosine Kinase 3 / Q000378:metabolism*
D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D017382:Reactive Oxygen Species; D019707:Receptors, Chemokine; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II; D016186:Receptor, Macrophage Colony-Stimulating Factor; D051941:fms-Like Tyrosine Kinase 3