[Cloning and characterization of a transcription factor ZmNAC1 in maize (Zea mays)].
NAC transcription factors are a family of functionally diverse proteins. They are unique to plants and play an important role in regulation of plant growth and development, hormone regulation and responses to various stresses. A cDNA encoding the NAC-like gene homologue was isolated from maize (Zea mays L.) by RT-PCR and designated ZmNAC1 (GenBank Accession No. EU224278). Sequence analysis showed that cDNA of ZmNAC1 was 1,029 bp long and contained a single open reading frame (ORF, 26 to approximately 907 bp). The predicted ZmNAC1 protein has 293 amino acids with an estimated molecular mass of 32.3 kDa and an isoelectric point of 8.65. RT-PCR analysis showed that the expression of ZmNAC1 was induced by low temperature, PEG, salt, and ABA, respectively. These results suggest that ZmNAC1 may play important roles in biotic and abiotic resistance pathways. This is the first NAC-like gene reported in maize.
Liu
Zhan-Ji
ZJ
Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences; Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Jinan 250100, China. liuzhanji@yahoo.com.cn
Shao
Feng-Xia
FX
Tang
Gui-Ying
GY
Shan
Lei
L
Bi
Yu-Ping
YP
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C418004:NAC1 protein, Arabidopsis; D015534:Trans-Activators; D014157:Transcription Factors