[The expression of GATA-4 and Nkx2.5 gene in the transformation of Rattus mesenchymal stem cells into cardiomyocytes].
OBJECTIVE
To investigate the expression of the transcriptive factors, GATA-4 and Nkx2.5 gene, in the course of induction of mesenchymal stem cells (MSCs) into cardiomyocytes, and analyze its molecular biological mechanism.


METHODS
Rattus Mesenchymal Stem Cells were cultured in vitro and 3rd generation cells were induced in 10 micromol/L 5-azacytidine, troponin I was checked with immunohistochemistry and Western blot, GATA-4 and NkX2.5 gene were analyzed by RT-PCR in 1, 2, 3 and 4 weeks respectively.


RESULTS
Growth of MSCs stopped after being induced, trending to form colonies, troponin I positive expressed since 2nd week, GATA-4 and Nkx2.5 began to express in 1st week, increasing gradually, peak in 3rd week.


CONCLUSIONS
GATA-4 and Nkx2. 5 gene expression increased gradually in the course of stem cells differentiation, which may control the rule of cardiomyocyte transcription.
Wu
Chang-xue
CX
Department of Thoracic and Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Er-yong
EY
Yang
Si-yuan
SY
Ma
Jian-yang
JY
An
Qi
Q
Shi
Ying-kang
YK
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D050991:GATA4 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D050991:GATA4 Transcription Factor; C084365:Gata4 protein, rat; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C524161:Nkx2-5 protein, rat; D014157:Transcription Factors