title: Crystallization and preliminary X-ray analysis of human liver alpha-enolase.
abstract: Enolase is a multifunctional enzyme that plays important roles in many biological and disease processes. alpha-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by affinity column chromatography and gel filtration. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 72.85, b = 66.02, c = 79.43 A, beta = 94.54 degrees .
authors.affiliation: Peking University, Beijing, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Yan Feng
authors.initials: YF
authors.lastname: Zhou
authors.forename: Lan Fen
authors.initials: LF
authors.lastname: Li
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry*; D002846:Chromatography, Affinity; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000737:chemistry*; D016415:Sequence Alignment; D025521:Tumor Suppressor Proteins / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase