title: Screening of food grade lipases to be used in esterification and interesterification reactions of industrial interest.
abstract: Seven food grade commercially available lipases were immobilized by covalent binding on polysiloxane-polyvinyl alcohol (POS-PVA) hybrid composite and screened to mediate reactions of industrial interest. The synthesis of butyl butyrate and the interesterification of tripalmitin with triolein were chosen as model reactions. The highest esterification activity (240.63 microM/g min) was achieved by Candida rugosa lipase, while the highest interesterification yield (31%, in 72 h) was achieved by lipase from Rhizopus oryzae, with the production of about 15 mM of the triglycerides C(50) and C(52). This lipase also showed a good performance in butyl butyrate synthesis, with an esterification activity of 171.14 microM/g min. The results demonstrated the feasibility of using lipases from C. rugosa for esterification and R. oryzae lipase for both esterification and interesterification reactions.
authors.affiliation: Engineering School of Lorena, University of São Paulo, P.O. Box 116, Lorena, 12602-810 São Paulo, Brazil.
authors.forename: Ariela Veloso
authors.initials: AV
authors.lastname: de Paula
authors.forename: Gisele Fátima Morais
authors.initials: GF
authors.lastname: Nunes
authors.forename: Josiane de Lourdes
authors.initials: Jde L
authors.lastname: Silva
authors.forename: Heizir Ferreira
authors.initials: HF
authors.lastname: de Castro
authors.forename: Júlio César
authors.initials: JC
authors.lastname: dos Santos
mesh_terms: D002087:Butyrates / Q000378:metabolism; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification / Q000502:physiology; D005524:Food Technology / Q000379:methods; D008049:Lipase / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology; D014280:Triglycerides / Q000378:metabolism; D014304:Triolein / Q000378:metabolism
chemical_list: D002087:Butyrates; D004800:Enzymes, Immobilized; D014280:Triglycerides; D014304:Triolein; C022793:n-butyl n-butyrate; C005859:tripalmitin; D008049:Lipase