Characterization of a CD44/CD122int memory CD8 T cell subset generated under sterile inflammatory conditions.
Most memory CD8 T cell subsets that have been hitherto defined are generated in response to infectious pathogens. In this study, we have characterized the CD8 T cells that survive priming conditions, devoid of pathogen-derived danger signals. In both a TCR-transgenic model and a model of contact hypersensitivity, we show that the priming of naive CD8 T cells under sterile inflammatory conditions generates memory. The corresponding memory CD8 T cells can be identified by their intermediate expression levels of CD44 and CD122. We also show that CD44/122(int) memory CD8 T cells spontaneously develop in wild type mice and that they display intermediate levels of several other memory traits including functional (IFN-gamma secretion capacity, CCL5 messenger stores), phenotypic, and molecular (T-bet and eomesodermin expression levels) features. We finally show that they correspond to an early differentiation stage and can further differentiate in CD44/122(high) memory T cells. Altogether, our results identify a new memory CD8 T cell subset that is generated under sterile inflammatory conditions and involved in the recall contact hypersensitivity reactions that are responsible for allergic contact dermatitis.
Mbitikon-Kobo
Florentin-Martial
FM
Université de Lyon, France.
Vocanson
Marc
M
Michallet
Marie-Cécile
MC
Tomkowiak
Martine
M
Cottalorda
Anne
A
Angelov
Georgi S
GS
Coupet
Charles-Antoine
CA
Djebali
Sophia
S
Marçais
Antoine
A
Dubois
Bertrand
B
Bonnefoy-Bérard
Nathalie
N
Nicolas
Jean-François
JF
Arpin
Christophe
C
Marvel
Jacqueline
J
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000502:physiology*; D007156:Immunologic Memory / Q000235:genetics*; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology*; D053646:Interleukin-2 Receptor beta Subunit / Q000096:biosynthesis / Q000502:physiology*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D015415:Biomarkers; C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; C508022:Il2rb protein, mouse; D018836:Inflammation Mediators; D053646:Interleukin-2 Receptor beta Subunit