Beneficial effect of autologous endometrial cell coculture in patients with repeated implantation failure.
OBJECTIVE
To confirm the beneficial effect of endometrial coculture in patients with repeated failures with assisted reproductive techniques (ART).


METHODS
Patients with previous failures were offered a repetition of ART in conjunction with autologous endometrial coculture.


METHODS
Private fertility center.


METHODS
Sixty-eight couples who had attempted 92 cycles of IVF or intracytoplasmic sperm injection without obtaining an evolutive pregnancy.


METHODS
Patients repeated one cycle of ART with concomitant endometrial coculture of their embryos.


METHODS
Comparative pregnancy and delivery rates in conventional ART cycles vs. cycles with autologous endometrial coculture.


RESULTS
In the previous 92 cycles (146 ETs, fresh plus frozen) only 8 pregnancies were initiated, and all ended in spontaneous abortion. Upon repeating 68 cycles (76 ETs) using coculture, 39 pregnancies were obtained, of which 19 resulted in live births, 10 are ongoing evolutive pregnancies, and 10 ended in spontaneous abortions.


CONCLUSIONS
These results confirm the usefulness of autologous endometrial coculture for the treatment of patients with repeated implantation failure.
Eyheremendy
Victoria
V
Centro Médico Fertilab, Buenos Aires, Argentina.
Raffo
Fernanda G E
FG
Papayannis
Mercedes
M
Barnes
Julia
J
Granados
Cintia
C
Blaquier
Jorge
J
D000328:Adult; D018920:Coculture Techniques; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic