title: Bilateral periprosthetic joint infection caused by Salmonella enterica serotype Enteritidis, and identification of Salmonella sp using molecular techniques.
abstract: Salmonella septic arthritis is rare. Our objective was to identify bacterial species from joint fluid using a broad-range real-time PCR and pyrosequencing technique. We describe a case of bilateral Salmonella enterica serotype Enteritidis infection of right and left total knee arthroplasties. DNA was extracted from the joint fluid of the left knee, amplified by PCR, and the amplicons were evaluated by pyrosequencing. The patient was treated with ciprofloxacin, and the polyethylene liners were replaced in both knees. The results of pyrosequencing detected a Salmonella species. To the best of our knowledge, this is the first report describing the detection of Salmonella in joint fluid by universal PCR followed by pyrosequencing.
authors.affiliation: Pathology and Laboratory Medicine Institute, The Cleveland Clinic, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.
authors.forename: Hideo
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Gerri S
authors.initials: GS
authors.lastname: Hall
authors.forename: Marion J
authors.initials: MJ
authors.lastname: Tuohy
authors.forename: Ulf
authors.initials: U
authors.lastname: Knothe
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Bauer
mesh_terms: D000368:Aged; D019645:Arthroplasty, Replacement, Knee / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007595:Joint Prosthesis / Q000382:microbiology; D007719:Knee Joint / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology; D012480:Salmonella Infections / Q000175:diagnosis* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA / Q000379:methods*