title: Effects of temperature on gamete longevity and fertilization success in two sea urchin species, Echinometra mathaei and Tripneustes gratilla.
abstract: We investigated gamete longevity in two sea urchin species, Echinometra mathaei (Em) and Tripneustes gratilla (Tg), via laboratory experiments. The longevity of dry sperm at different ages (6, 12, 18, 24, and 30 h after collection) was tested at 20 degrees, 25 degrees, and 30 degrees C. Sperm viability was determined by the percent cleavage of eggs after in-vitro fertilization. Dry sperm of Em remained viable longer than dry sperm of Tg at all temperatures. The viability of dilute sperm and eggs was also determined at 20 degrees, 25 degrees, and 30 degrees C over varying durations (for sperm, 5, 45, and 85 min; for eggs, 1, 3, 6, 12, and 24 h). The viability of dilute Em sperm remained relatively constant over time (nearly 100%) at 20 degrees and 25 degrees C, but decreased rapidly at 30 degrees C. The viability of Tg sperm declined gradually over time at 20 degrees C and decreased sharply at 25 degrees and 30 degrees C. Eggs of Em and Tg remained fertilizable for 12 and 6 h, respectively. Most eggs of Em and Tg underwent normal development when exposed for less than 6 h and 3 h, respectively. These results may illuminate the different fertilization strategies of these two sea urchin species.
authors.affiliation: Department of Marine and Environmental Science, Graduate School of Engineering and Science, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-0213, Japan. r7saif@yahoo.com
authors.forename: Md Saifur
authors.initials: MS
authors.lastname: Rahman
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uehara
mesh_terms: D000818:Animals; D005306:Fertilization / Q000502:physiology*; D005854:Germ Cells / Q000502:physiology*; D012617:Sea Urchins / Q000502:physiology*; D013696:Temperature; D013997:Time Factors