title: DNA context and promoter activity affect gene expression in lentiviral vectors.
abstract: Several varieties oflentiviral delivery systems have been used to generate stable cell lines and transgenic animals. Some enhancing elements have been identified to promote stable transgene expression. In this study, we describe that the promoter activity is affected by the lentiviral genomic context. We have examined the promoter activities using green fluorescence protein (GFP) as a reporter in different cell lines to demonstrate that the cytomegalovirus (CMV) promoter may not always be the best choice to overexpress transgenes in all cell types. Our data showed that the polypeptide chain elongation factor 1 alpha (EF1alpha) promoter is relatively active in all three model cell lines (293T, HOS, and Hela) while the CMV promoter is less effective in Hela cells.
authors.affiliation: Department of Neurosurgery, Chinese People's Armed Police Forces General Hospital, Beijing 100039, China.
authors.forename: Gensheng
authors.initials: G
authors.lastname: Mao
authors.forename: Francesco
authors.initials: F
authors.lastname: Marotta
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.forename: Dehua
authors.initials: D
authors.lastname: Chui
mesh_terms: D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014161:Transduction, Genetic
chemical_list: D004279:DNA, Viral