[Effect of low-intensity laser radiation on the function of vascular endothelium in patients with chronic viral hepatitis].
The aim of the study was to measure plasma levels of stable metabolites of nitric oxide, nitrates, and nitrites (NOx) in patients with chronic viral hepatitis and evaluate the possibility of their correction by low-power laser irradiation. NO metabolites (total nitrites and nitrates) were measured colorimetrically from the development of colour in the reaction of nitrite with sulfanilamide diazotization in Griess reagent. Colour intensity was determined with Victor2 enzyme immunoanalyzer, Perkin Elmaer (Finland). The patients were divided into three groups. In group 1 (control, n = 30) they received combined medicamentous therapy, in group 2 (n = 45) medicamentous therapy and a course of intravenous laser therapy, in goup 3 (n = 45) medicamentous therapy and skin laserotherapy. The results indicate that medicamentous treatment of patients with chronic hepatitis does not bring any beneficial changes in plasma NOx whose levels are significantly improved in case of simultaneous laser therapy. It is concluded that different laserotherapeutic modalities have beneficial effect on NO-producing function of endothelium and thereby improve its functional state. Compensation of NO deficit by laser therapy ensures overall protection of the organism against free radicals and decreases severity of oxidative stress.
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D000328:Adult; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology* / Q000528:radiation effects; D005260:Female; D005500:Follow-Up Studies; D019694:Hepatitis B, Chronic / Q000378:metabolism / Q000503:physiopathology / Q000532:radiotherapy*; D019698:Hepatitis C, Chronic / Q000378:metabolism / Q000503:physiopathology / Q000532:radiotherapy*; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000528:radiation effects*; D016896:Treatment Outcome; D014664:Vasodilation / Q000502:physiology / Q000528:radiation effects*
D009569:Nitric Oxide