title: Partially hydrolyzed guar gum supplement reduces high-fat diet increased blood lipids and oxidative stress and ameliorates FeCl3-induced acute arterial injury in hamsters.
abstract: Increased reactive oxygen species (ROS) and hyperlipidemia can promote arterial thrombus. We evaluated the potential of a partially hydrolyzed guar gum (PHGG) as dietary fiber on lipid profiles and FeCl3-induced arterial thrombosis in the high fat-diet fed hamsters. Our in vitro results found that PHGG is efficient to scavenge O2-*, H2O2, and HOCl. High fat-diet increased plasma triglyceride, total cholesterol, LDL, VLDL, methylguanidine and dityrosine level and accelerated FeCl3-induced arterial thrombosis formation (from 463 +/- 51 to 303 +/- 45 sec). Low dose PHGG supplement significantly decreased the total cholesterol, LDL, methylguanidine and dityrosine level and delayed the time for arterial thrombosis formation (528 +/- 75 sec). High dose PHGG supplement decreased the level in triglyceride, total cholesterol, LDL and VLDL and further delayed the time for arterial thrombus (671 +/- 36 sec). The increased Bax protein and decreased Bcl-2 and HSP-70 protein expression was found in the carotid and femoral arteries of high fat-diet hamsters. Low and high dose of PHGG supplement decreased Bax expression and increased Bcl-2 and HSP-70 protein expression. We found that FeCl3 significantly enhanced intercellular adhesion molecule-1 and 4-hydroxynonenal expression in the endothelial site of damaged artery after 150-sec FeCl3 stimulation. PHGG supplement decreased the endothelial ICAM-1 and 4-hydroxynonenal expression after 150-sec FeCl3 stimulation. Based on these results, we conclude that PHGG supplement can increase antioxidant protein expression and thus decrease oxidative stress induced arterial injury.
authors.affiliation: Department of Cardiovascular Surgery, Kuang-Tien General Hospital, Taichung, Taiwan. 2542365@ms27.hinet.net
authors.forename: Dar-Chih
authors.initials: DC
authors.lastname: Kuo
authors.forename: Shih-Ping
authors.initials: SP
authors.lastname: Hsu
authors.forename: Chiang-Ting
authors.initials: CT
authors.lastname: Chien
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001158:Arteries / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D001835:Body Weight; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006224:Cricetinae; D004041:Dietary Fats; D019587:Dietary Supplements; D004435:Eating; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000378:metabolism; D005685:Galactans / Q000008:administration & dosage / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D058085:Iron Compounds; D008055:Lipids / Q000097:blood*; D008297:Male; D008351:Mannans / Q000008:administration & dosage / Q000378:metabolism; D008647:Mesocricetus; D008969:Molecular Sequence Data; D015394:Molecular Structure; D018384:Oxidative Stress; D053149:Plant Gums / Q000008:administration & dosage / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013927:Thrombosis / Q000378:metabolism / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000447:Aldehydes; D000975:Antioxidants; D004041:Dietary Fats; D016166:Free Radical Scavengers; D005685:Galactans; D018840:HSP70 Heat-Shock Proteins; D058085:Iron Compounds; D008055:Lipids; D008351:Mannans; D053149:Plant Gums; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D018799:Intercellular Adhesion Molecule-1; C007894:guar gum; C027576:4-hydroxy-2-nonenal