Universal detection and identification of avian influenza virus by use of resequencing microarrays.
Zoonotic microbes have historically been, and continue to emerge as, threats to human health. The recent outbreaks of highly pathogenic avian influenza virus in bird populations and the appearance of some human infections have increased the concern of a possible new influenza pandemic, which highlights the need for broad-spectrum detection methods for rapidly identifying the spread or outbreak of all variants of avian influenza virus. In this study, we demonstrate that high-density resequencing pathogen microarrays (RPM) can be such a tool. The results from 37 influenza virus isolates show that the RPM platform is an effective means for detecting and subtyping influenza virus, while simultaneously providing sequence information for strain resolution, pathogenicity, and drug resistance without additional analysis. This study establishes that the RPM platform is a broad-spectrum pathogen detection and surveillance tool for monitoring the circulation of prevalent influenza viruses in the poultry industry and in wild birds or incidental exposures and infections in humans.
Lin
Baochuan
B
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375, USA. baochuan.lin@nrl.navy.mil
Malanoski
Anthony P
AP
Wang
Zheng
Z
Blaney
Kate M
KM
Long
Nina C
NC
Meador
Carolyn E
CE
Metzgar
David
D
Myers
Christopher A
CA
Yingst
Samuel L
SL
Monteville
Marshall R
MR
Saad
Magdi D
MD
Schnur
Joel M
JM
Tibbetts
Clark
C
Stenger
David A
DA
D000818:Animals; D001717:Birds; D009980:Influenza A virus / Q000145:classification* / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
D012367:RNA, Viral