A new automated multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) using an AP720S analyzer.
BACKGROUND
In the diagnosis of atopic diseases, allergen detection is a crucial step. Multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is a simple and noninvasive method for in vitro screening of allergen-specific IgE antibodies.


METHODS
The Korean Inhalant Panel test on 20 patients and Food Panel test on 19 patients were performed using the conventional manual MAST-CLA kit and the new automated MAST-CLA method (automated AP720S system for the Optigen Assay; Hitachi Chemical Diagnostics, Inc., USA) simultaneously. The results were evaluated for positive reactivity and concordance.


RESULTS
The results of inhalant panel gave a relatively higher class level result than the food panel. The 8 patients out of 20 (40%) of the inhalation panel, and 9 patients out of 18 (47.4%) of the food panel showed 100% concordance between the 2 systems. Eighteen patients (90%) of the Inhalation Panel and sixteen patients (84.2%) of the Food Panel showed more than 91% concordance.


CONCLUSIONS
These results suggest that the MAST-CLA assay using the new, automated AP720S analyzer performs well, showing a high concordance rate with conventional MAST-CLA. Compared to manual MAST-CLA, the automated AP720S system has a shorter assay time and uses a smaller serum volume (500 microl) along with other conveniences.
Lee
Sungsil
S
Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
Lim
Hwan Sub
HS
Park
Jungyong
J
Kim
Hyon Suk
HS
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000032:analysis*; D002648:Child; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D000485:Allergens; D007073:Immunoglobulin E