[Lentinan-graft-polyethylenimine-a novel vector for gene delivery].
To develop a novel vector for gene delivery with low molecular weight polyethylenimine grafted to the natural polysaccharide and conjugated to folic acid (LNT-PEI-FA).



The properties of LNT-PEI-FA were characterized by (1)H-NMR, FT-IR and TGA, respectively. The particle size of LNT-PEI-FA/DNA complex was measured. The DNA binding ability of LNT-PEI-FA was detected by gel electrophoresis retardation assay.



The particle size of LNT-PEI-FA/DNA complex was about 200 nm. Gel electrophoresis showed that at N/P ratio of 1.8 (W/W) the polymer was able to completely condense DNA. In vitro experiments showed a high efficiency of gene transfection in A293 and B16 cell lines.



A novel non-viral vector LNT-PEI-FA was successfully synthesized and characterized, which may be applied in gene transfection research in the future.
Jiang
Qi-Ying
QY
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
Lu
Xiao
X
Hu
Yi-Ping
YP
Chen
Dan
D
Zhao
Dan-Jun
DJ
Zhou
Jun
J
Yu
Hai
H
Wang
Qing-Qing
QQ
Tang
Gu-Ping
GP
D002460:Cell Line; D005492:Folic Acid / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D007912:Lentinan / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
D007912:Lentinan; D011094:Polyethyleneimine; D005492:Folic Acid