title: Differential expression of surface glycoconjugates on Entamoeba histolytica and Entamoeba dispar.
abstract: The human large intestine can harbor two morphologically similar amoebae; the invasive Entamoeba histolytica and the non-invasive Entamoeba dispar. Whereas E. histolytica can produce intestinal and extra-intestinal lesions, E. dispar is present in non-symptomatic carriers. Although biochemical, genetic and proteomic studies have identified clear differences between these Entamoebae, it has become clear that several molecules, once assumed to be involved in tissue destruction, exist in both the virulent and the avirulent species. As surface molecules may play a role in invasion and could therefore determine which amoebae are invasive, we analyzed the glycoconjugate composition of E. histolytica and E. dispar using lectins. There was a significant difference between E. histolytica and E. dispar in the expression of glycoconjugates containing d-mannose and N-acetyl-alpha-D-galactosamine residues, but not between virulent and avirulent strains of E. histolytica. N-glycoconjugates with terminal alpha (1-3)-linked mannose residues participate in the adhesion and subsequent cytotoxicity of E. histolytica to cultured hamster hepatocytes. One of them probably is the Gal/GalNAc lectin.
authors.affiliation: Electron Microscopy Laboratory, Mexican Faculty of Medicine, La Salle University, Mexico City, Mexico.
authors.forename: Judith
authors.initials: J
authors.lastname: Pacheco-Yépez
authors.forename: Rafael
authors.initials: R
authors.lastname: Campos-Rodríguez
authors.forename: Saúl
authors.initials: S
authors.lastname: Rojas-Hernández
authors.forename: José J
authors.initials: JJ
authors.lastname: Serrano-Luna
authors.forename: Víctor
authors.initials: V
authors.lastname: Rivera-Aguilar
authors.forename: Saúl
authors.initials: S
authors.lastname: Villa-Treviño
authors.forename: Adolfo
authors.initials: A
authors.lastname: Martínez-Palomo
authors.forename: Víctor
authors.initials: V
authors.lastname: Tsutsumi
authors.forename: Mineko
authors.initials: M
authors.lastname: Shibayama
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D006224:Cricetinae; D004747:Entamoeba / Q000145:classification / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D004748:Entamoeba histolytica / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D004749:Entamoebiasis / Q000469:parasitology / Q000473:pathology; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism*; D022781:Hepatocytes / Q000469:parasitology / Q000473:pathology; D008101:Liver Abscess, Amebic / Q000469:parasitology / Q000473:pathology; D008358:Mannose / Q000032:analysis; D014774:Virulence
chemical_list: D006001:Glycoconjugates; D000116:Acetylgalactosamine; D008358:Mannose