Alteration of Ca(2+)-ATPase activity in the homogenate, plasma membrane and microsomes of the salivary glands of streptozotocin-induced diabetic rats.
Diabetes has been implicated in the dryness of the mouth, loss of taste sensation, sialosis, and other disorders of the oral cavity, by impairment of the salivary glands. The aim of the present study was to examine the plasma membrane, microsomal, and homogenate Ca(2+)-ATPase activity in the rat submandibular and parotid salivary glands of streptozotocin-induced diabetes. We have also examined the influence of the acidosis state on this parameter. Diabetes was induced by an intraperitoneal injection of streptozotocin and acidosis was induced by daily injection of NH(4)Cl. At 15 and 30 days after diabetes induction, the animals were euthanized and the submandibular and parotid salivary glands were removed and analyzed. Ca(2+)-ATPase (total, independent, and dependent) was determined in the homogenate, microsomal, and plasma membranes of the salivary glands of diabetic and control rats. Calcium concentration was also determined in the glands and showed to be higher in the diabetic animals. Ca(2+)-ATPase activity was found to be reduced in all cell fractions studied in the diabetic animals compared with control. Similar results were obtained for the submandibular salivary glands of acidotic animals; however in the parotid salivary glands it was found an increase in the enzyme activity.
Nicolau
José
J
Oral Biology Research Center, University of São Paulo, Brazil. jnicolau@usp.br
De Souza
Douglas Nesadal
DN
Simões
Alyne
A
D000138:Acidosis / Q000150:complications / Q000201:enzymology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D010306:Parotid Gland / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000166:cytology* / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology
D000252:Calcium-Transporting ATPases; D002118:Calcium