Quantum dots-based immunofluorescence technology for the quantitative determination of HER2 expression in breast cancer.
HER2 detection is important for breast cancer (BC) treatment and prognosis, but the detection methods currently used have some disadvantages. Quantum dots (QDs)-based probes provide a potentially important new method for HER2 detection in clinical practice. This potential is examined in this paper. A QDs HER2 probe kit and QDs image acquisition and analysis software were developed and applied to 94 clinical samples of BC. Compared to conventional immunohistochemistry techniques, this method provided a superior accurate and sensitive method for the detection of HER2 in clinical breast cancer diagnosis.
Chen
Chuang
C
Department of Oncology, Zhongnan Hospital of Wuhan University, Wuchang District, PR China.
Peng
Jun
J
Xia
He-Shun
HS
Yang
Gui-Fang
GF
Wu
Qiong-Shui
QS
Chen
Liang-Dong
LD
Zeng
Li-Bo
LB
Zhang
Zhi-Ling
ZL
Pang
Dai-Wen
DW
Li
Yan
Y
D001943:Breast Neoplasms / Q000235:genetics*; D005240:Feasibility Studies; D005455:Fluorescent Antibody Technique; D018734:Genes, erbB-2; D006801:Humans; D045663:Quantum Dots; D012680:Sensitivity and Specificity