[Impact of human isophane insulin on the evaluation of beta cell functions].
OBJECTIVE
To evaluate the beta cell functions with and without human Isophane insulin treatments.


METHODS
Thirty patients with type 2 diabetes mellitus who followed the human Isophane insulin therapeutic regimen at bedtime were given 100 g steamed bread tests in two consecutive days. Then the human Isophane insulin treatments were stopped and the 100 g steamed bread tests were repeated in the next day. The fasting and 1, 2, 3 h after meal plasma glucose, serum insulin, C-peptide and proinsulin were measured.


RESULTS
(1) The fasting and 1,2,3 h after meal plasma glucose increased while the serum insulin decreased after the human Isophane insulin treatments were stopped (P<0.05). (2) The fasting and 1, 2 h after meal C peptide increased (P<0.05) after the human Isophane insulin treatments were stopped. (3) The fasting and 1, 2, 3 h after meal proinsulin increased after the human isophane insulin treatments were stopped (P<0.05). (4) The area under the insulin curve decreased, while, the area under the C peptide increased after the human Isophane insulin treatments were stopped (P<0.05).


CONCLUSIONS
Human Isophane insulin treatments should not be stopped to evaluate the beta cell functions of the patients who are undergoing Isophane insulin treatment.
Zhang
Mei
M
Division of Hormone Assay, Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Lin
L
Huang
Ming-jie
MJ
Xiong
Zhong-yun
ZY
Guo
Dan
D
An
Zhen-mei
ZM
D000328:Adult; D000368:Aged; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism; D002096:C-Peptide / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000503:physiopathology*; D005260:Female; D005951:Glucose Tolerance Test / Q000379:methods; D006801:Humans; D007328:Insulin / Q000097:blood; D007336:Insulin, Isophane / Q000008:administration & dosage*; D050417:Insulin-Secreting Cells / Q000502:physiology*; D008297:Male; D008875:Middle Aged
D001786:Blood Glucose; D002096:C-Peptide; D007328:Insulin; D007336:Insulin, Isophane