Crystallization and preliminary X-ray diffraction analysis of a putative two-domain-type laccase from a metagenome.
A putative two-domain-type laccase retrieved from a metagenome was successfully crystallized using the sitting-drop vapour-diffusion method. Data were collected to a resolution of 1.7 A at 100 K using synchrotron radiation. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.67, b = 100.95, c = 124.11 A. The self-rotation function showed the presence of a noncrystallographic threefold axis in the structure. The presence of one trimer in the asymmetric unit yielded a Matthews coefficient (V(M)) of 2.05 A(3) Da(-1) and a solvent content of 40%.
Komori
Hirofumi
H
Department of Life Science, University of Hyogo, Koto, Kamigori-cho, Ako-gun, Japan.
Miyazaki
Kentaro
K
Higuchi
Yoshiki
Y
D003460:Crystallization; D018360:Crystallography, X-Ray; D016678:Genome / Q000235:genetics*; D042845:Laccase / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D042845:Laccase