[In vivo effect of recombined IL-15/Fc fusion protein on EAU].
OBJECTIVE
To test the effect of recombined IL-15/Fc on experimental autoimmune uveitis (EAU) in mice.


METHODS
EAU were induced in C57 mice by transferring activated T cells specific to the interphotoreceptor-binding protein (IRBP) 1-20 peptide. The mice were then treated with recombine IL-15/Fc fusion protein or IgG as controls. The severity of EAU were graded on a scale of 0 to 4 with half-point increment based on the type, number, and size of the lesions detected by funduscopic and HE staining. The IRBP1-20 sensitive CD8+T cells were isolated from the IRBP1-20 immune mice with auto-MACS. The in vitro effect of IL-15/Fc fusion protein on the proliferation, differentiation, expansion and production of inflammatory cytokines of the purified IRBP1-20 sensitive CD8+T cells were analyzed with 3HTdR, FACS and ELISA.


RESULTS
IL-15/Fc fusion protein inhibited the activation, proliferation, expansion and production of inflammatory cytokines of the IRBP1-20 specific CD8+T cells, down regulated CD44(high)CD62L(low) effect and CD8+ CD62L(low) activated T cell subsets, and consequently decreased the severity of EAU.


CONCLUSIONS
IL-15/Fc fusion proteins decrease the severity of EAU through inhibiting the proliferation, expansion, differentiation and production of inflammatory cytokines of CD8+ T cells.
Xia
Zong-jing
ZJ
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Kong
Xiang-li
XL
Zhang
Ping
P
D000818:Animals; D001327:Autoimmune Diseases / Q000188:drug therapy*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D005136:Eye Proteins / Q000276:immunology; D019409:Interleukin-15 / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use*; D012177:Retinol-Binding Proteins / Q000276:immunology; D014605:Uveitis / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D019409:Interleukin-15; D011993:Recombinant Fusion Proteins; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial; C440611:IL-15-Fcgamma2a protein, human