Glutathione s-transferase variants in a brazilian population.
Recent pharmacogenomic studies have revealed significant interethnic differences in glutathione S-transferase (GST) allelic frequencies among various ethnic groups. Therefore, we have investigated GSTM1 (gene deletion), GSTT1 (gene deletion) and GSTP1 (rs1695) polymorphism frequencies in 3 Brazilian ethnic groups (n = 203). GSTM1 and GSTT1 polymorphism analyses were performed by multiplex polymerase chain reaction, and GSTP1 (rs1695) analysis was done by polymerase chain reaction restriction fragment length polymorphism. GSTM1- polymorphism frequency was 33.2%, while GSTT1 null (GSTT1-) was 30.2%. The valine GSTP1*B (rs1695) allele was present in 35.1% subjects, while the heterozygous form (isoleucine/valine) was the most prevalent genotype (46.6%). We found a statistically significant difference in genotype frequency among Amerindians versus Caucasians (p = 0.016) and among Amerindians versus African-Americans (p = 0.033). Considerable frequency variation was found in our study, even when compared with other studies showing phylogeographical heterogeneity to the genes studied in Brazilian populations.
Magno
Luiz Alexandre V
LA
Laboratório de Farmacogenômica e Epidemiologia Molecular, Universidade Estadual de Santa Cruz, Ilhéus, Brazil.
Talbot
Jhimmy
J
Talbot
Teddy
T
Borges Santos
Alex Marques
AM
Souza
Renan P
RP
Marin
Lauro J
LJ
Moreli
Marcos Lázaro
ML
de Melo
Paulo R S
PR
Corrêa
Ronan X
RX
Rios Santos
Fabrício
F
Di Pietro
Giuliano
G
D000328:Adult; D001741:Black or African American / Q000235:genetics; D001938:Brazil / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007199:Indians, South American / Q000235:genetics; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D044465:White People / Q000235:genetics
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1