Generation of immune inhibitory dendritic cells and CD4+T regulatory cells inducing by TGF-beta.
Variety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. In this research, we studied the capacity of dendritic cells to expand antigen-specific T regulatory cells.We also investigated the role of TGF-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions.Dendritic cells were generated from blood CD14(+) monocytes with granulocyte-Monocyte colony stimulating factor and interleukin-4 with or without TGF-beta (TGF-beta-GM-DC or GM-DC). CD4(+) T cell were isolated to assess lymphocyte proliferation by lymphocyte transformation test assay and the ratio of CD4(+)FOXp3(+) CD25(+) T cells were determined by fluorescene-activated cell sorter. T cell proliferation responses in GM-DC showed a significance antigen-specific proliferative response comparing with TGFbeta-GM -DC. T Cell proliferation was inhibited in co-culture system containing DC-treated TGF-beta. It can be suggested that the expsansion of T regulatory by TGF-beta-GM-DC provides a means for antigen specific control of unwanted immune reactions.
Abediankenari
Saeid
S
Department of Microbiology and Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. abedianlab@yahoo.co.uk
Ghasemi
Maryam
M
D015703:Antigens, CD / Q000032:analysis; D051940:B7-2 Antigen / Q000032:analysis; D003713:Dendritic Cells / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology*; D051858:Forkhead Transcription Factors / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects* / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008562:Membrane Glycoproteins / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D000097802:CD83 Antigen
D015703:Antigens, CD; D051940:B7-2 Antigen; D051858:Forkhead Transcription Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D053645:Interleukin-2 Receptor alpha Subunit; D015847:Interleukin-4; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D016212:Transforming Growth Factor beta; D000097802:CD83 Antigen; C497964:CD86 protein, human; C418974:FOXP3 protein, human