Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2.
Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.
Mikkelsen
Michael Dalgaard
MD
VKR Research Centre Pro-Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
Fuller
Victoria L
VL
Hansen
Bjarne Gram
BG
Nafisi
Majse
M
Olsen
Carl Erik
CE
Nielsen
Henrik Bjørn
HB
Halkier
Barbara Ann
BA
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005961:Glucosinolates / Q000737:chemistry / Q000378:metabolism; D007210:Indoleacetic Acids / Q000737:chemistry / Q000378:metabolism; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D015394:Molecular Structure; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010091:Oximes / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism; D000094162:Phytoalexins
D029681:Arabidopsis Proteins; D003577:Cytochrome P-450 Enzyme System; D000431:Ethanol; D005961:Glucosinolates; D007210:Indoleacetic Acids; D007211:Indoles; D010091:Oximes; D013844:Thiazoles; C102405:camalexin; D000094162:Phytoalexins; C467098:indole-3-acetaldoxime; C030737:indoleacetic acid; C530432:cytochrome P-450 79B2 protein, Arabidopsis; C431937:CYP83B1 protein, Arabidopsis; C471054:cytochrome P-450 CYP79B3, Sinapis alba