[Effects of rh-leptin on the proliferation and migration of orbital preadipocytes of thyroid associated ophthalmopathy].
OBJECTIVE
To investigate the effects of rh-leptin on the proliferation, migration of orbital preadipocytes of thyroid associated ophthalmopathy (TAO).


METHODS
Preadipocytes were cultured from orbital tissues of TAO patients after orbital decompression. The subcultured preadipocytes were incubated with different concentrations of rh-leptin. The proliferation of preadipocytes was tested by methyl thiazlyl tetrazolium (MTT) assay, and the migration was evaluated by crutch method.


RESULTS
With the treatment of 500 mg/L rh-leptin, the preadipocytes proliferated more than control (P<0.05), while 10-100 mg/L rh-leptin has no such significant effects. The migration of the preadipocytes was enhanced by rh-leptin, which showed statistic difference in 50-500 mg/L rh-leptin groups when compared with control group (P<0.05).


CONCLUSIONS
Rh-leptin could improve the proliferation and migration of TAO orbital preadipocytes in vitro, which suggests that leptin may play an important role in the pathogenesis of TAO.
Tang
Jian
J
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.
Luo
Qing-li
QL
He
Wei-min
WM
D017667:Adipocytes / Q000473:pathology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D049970:Graves Ophthalmopathy / Q000473:pathology*; D006801:Humans; D020738:Leptin / Q000235:genetics / Q000494:pharmacology*; D009915:Orbit / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology
D020738:Leptin; D011994:Recombinant Proteins