title: Immunosuppressive function of bone marrow mesenchymal stem cells on acute rejection of liver allografts in rats.
abstract: Bone marrow mesenchymal stem cells (MSCs) demonstrate functions of immunologic regulation. However, little is known about the role of interferon-gamma (IFN-gamma) on MSCs and whether MSCs alone can prevent allograft rejection. We purified MSCs, which were or were not treated with IFN-gamma, to act as regulatory cells in mixed lymphocyte reactions. We measured their expression of PDL-1, MHC-I, MHC-II, CD40, CD54, and CD86. The MSCs stained with carboxyfluorescein diacetate-succinimidyl ester were used to detect homing in vivo. The MSCs were injected into an orthotopic liver transplantation model. The result suggested that IFN-gamma enhances expression of PDL-1, MHC-I, MHC-II, and CD54 and boosts immunosuppressive ability in vivo. The MSCs demonstrated homing to the liver, alleviating acute immunologic rejection of an hepatic graft in rats. We conclude that IFN-gamma may enhance the immunosuppressive function of MSCs to protect liver allografts in rats from acute immunologic rejection.
authors.affiliation: Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Xiamen, China.
authors.forename: Z-F
authors.initials: ZF
authors.lastname: Hong
authors.forename: X-J
authors.initials: XJ
authors.lastname: Huang
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Yin
authors.forename: W-X
authors.initials: WX
authors.lastname: Zhao
authors.forename: X-M
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006084:Graft Rejection / Q000276:immunology* / Q000517:prevention & control; D007165:Immunosuppression Therapy; D017493:Leukocyte Common Antigens / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D011914:Rats, Inbred BN; D051876:Sex-Determining Region Y Protein / Q000235:genetics; D018800:Thy-1 Antigens / Q000276:immunology; D018183:Transplantation Chimera; D014998:Y Chromosome
chemical_list: D000939:Epitopes; D051876:Sex-Determining Region Y Protein; C497747:Sry protein, rat; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens