[Effects of manganese exposure on liver in rats].
OBJECTIVE
To observe effects of manganese on function and pathology of liver in rats.


METHODS
36 male SD rats were randomly divited into 3 groups, each group contained 12 rats. Solutions contained MnCl2 of 5.0 g/L and 0.5 g/L were respectively administrated to the high dose and low dose Mn-exposed groups consecutively by oral drinking for 60 days, while distilled water was given to the contal group. Fluorospectrophotometry was carried out to exame the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), as well as the protein level in liver plasmas. HE staining for the liver sections was applied to exam the pathological changes of liver in rats.


RESULTS
(1) In fluorospe-ctrophotometry, activities of ALT, AST, ALP in high and low dose Mn-exposed groups were significantly higher than those in control group in liver plasmas. In high dose Mn-exposed groups, ALT, AST, ALP was respectively (60.79 +/- 9.88), (49.77 +/- 8.36) and (49.43 +/- 8.88) U/ml, P < 0.01, in low dose Mn-exposed groups, ALT,AST,ALP was respectively (47.35 +/- 8.11), (37.31 +/- 6.77) and (32.34 +/- 4.53) U/ml (P < 0.05). Significant relationships between effects and dose were obvious in the above changes. No changes in PRO levels was found among 3 groups. (2) Pathologically in high dose and low dose Mn-exposed groups the hepatic lobules were injured in different levels. Mainly the arrangements of some hepatic cords became loose or disordered and liver cells were somewhat different in shape and apparent volume. Some hepatic cells appeared lipopexic and numerous granulocytic immersion occurred around the central vein.


CONCLUSIONS
manganese exposure resulted in the poisonous effects on liver in rats.
Wu
Yanming
Y
Department of Basic Sciences, Guangdong Pharmaceutical College, Guangzhou 510006, China. yanmingjx@sina.com
Zhang
Dexing
D
He
Xinghong
X
Lu
Zhigen
Z
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D004781:Environmental Exposure / Q000009:adverse effects*; D043384:Glutamyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000473:pathology* / Q000502:physiology*; D008297:Male; D008345:Manganese / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D008345:Manganese; D000410:Alanine Transaminase; D043384:Glutamyl Aminopeptidase