The trigeminal retrograde transfer pathway in the treatment of neurodegeneration.
The trigeminal sensory system was evaluated for the retrograde transfer of gene therapy vectors into the CNS. The feline immunodeficiency viral vector, FIV(HEXB), encoding for the human HEXB gene, was injected intra-articularly in the temporomandibular joint of 12 week-old HexB(-/-) mice displaying clinical and histopathological signs of Sandhoff disease. This treatment regiment reduced GM(2) storage and ameliorated neuroinflammation in the brain of HexB(-/-) mice, as well as attenuated behavioral deficits. In conclusion, retrograde transfer along trigeminal sensory nerves may prove to be a valuable route of gene therapy administration for the treatment of lysosomal storage disorders and other neurodegenerative diseases.
Kyrkanides
Stephanos
S
Department of Children's Dentistry, Stony Brook University, Stony Brook, NY 11794-8700, USA. kyrkanides@gmail.com
Yang
Meixiang
M
Tallents
Ross H
RH
Miller
Jen-nie H
JN
Brouxhon
Sabine M
SM
Olschowka
John A
JA
D000818:Animals; D001370:Axonal Transport / Q000235:genetics; D001522:Behavior, Animal / Q000502:physiology; D004195:Disease Models, Animal; D004660:Encephalitis / Q000235:genetics / Q000628:therapy; D005678:G(M2) Ganglioside / Q000235:genetics / Q000378:metabolism; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics; D020140:Lysosomal Storage Diseases, Nervous System / Q000235:genetics / Q000628:therapy*; D051379:Mice; D018345:Mice, Knockout; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000628:therapy*; D012497:Sandhoff Disease / Q000235:genetics / Q000628:therapy; D016896:Treatment Outcome; D014276:Trigeminal Nerve / Q000166:cytology / Q000378:metabolism*; D054821:beta-Hexosaminidase beta Chain / Q000235:genetics*
D005678:G(M2) Ganglioside; C513386:HEXB protein, human; D054821:beta-Hexosaminidase beta Chain