Rapid chemiluminescence biosensing of microcystin-LR.
A rapid immunoassay for sensitive detection of microcystin-LR using a portable chemiluminescence multichannel immunosensor (CL-MADAG) was developed. The sensor device is based on a capillary ELISA technique in combination with a miniaturized fluidics system and uses chemiluminescence as the detection principle. Minimum concentrations of at least 0.2 microgL(-1) microcystin-LR could be unambiguously measured in a spiked buffer system as well as in spiked real water samples. A single sample analysis for detection of microcystin-LR could be accomplished in just 13 min on the CL-MADAG. Besides providing a highly reproducible, fast and easy to perform test format, one major advantage of the newly established capillary immunoassay is represented by the feasibility of an internal retrospective quality control mechanism. Finally, simultaneous CL-MADAG measurements employing our inhibition immunoassay and a sandwich ELISA could be successfully demonstrated.
Lindner
Petra
P
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Regensburg, Germany.
Molz
Ramona
R
Yacoub-George
Erwin
E
Wolf
Hans
H
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis*
D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; D012822:Silicon Dioxide; C057862:cyanoginosin LR