Expression of toll-like receptor 2 and 4 in intestinal mucosa in experimental severe acute pancreatitis.
OBJECTIVE
In severe acute pancreatitis (SAP), endotoxin/bacterial translocation (E/BT) from the gut is main cause of infection, which is contributor to high mortality. Toll-like receptors (TLRs) recognize micro-organisms and are involved in the host defense. This study aimed to investigate the expression of TLR2 and TLR4 in intestinal mucosa in SAP.


METHODS
SAP was induced by injection of 3% sodium deoxycholate into the biliopancreatic duct in rats. Terminal ileum was removed 2, 6, 12, and 18 hours after the induction. Expression of TLR2/4 was analyzed by western blotting and immunohistochemistry. NF-kappaB activation was assessed by enzyme immunoassay. E/BT was also evaluated.


RESULTS
TLR2/4 proteins were increased 2 and 6 hours, and were decreased 12 and 18 hours after the induction. Immunoreactivities for them were detected at the top of villi and crypt in control rats. They were increased 6 hours, and were diminished 12 hours after the induction. Activated NF-kappaB was increased 6 hours, and was decreased 18 hours after the induction. E/BT occurred 18 hours after the induction.


CONCLUSIONS
Intestinal immune response enhances in the early phase and suppresses in the late phase of SAP, and TLRs may be implicated in the mechanism of E/BT.
Sawa
Hidehiro
H
Department of Gastroenterological Surgery, Kobe University Graduate School of Medical Sciences, Kobe 650-0017, Japan. hdhrsawa@med.kobe-u.ac.jp
Ueda
Takashi
T
Takeyama
Yoshifumi
Y
Yasuda
Takeo
T
Shinzeki
Makoto
M
Matsumura
Naoki
N
Nakajima
Takahiro
T
Kuroda
Yoshikazu
Y
D000208:Acute Disease; D000818:Animals; D018988:Bacterial Translocation / Q000276:immunology; D015153:Blotting, Western; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism*; D008297:Male; D010195:Pancreatitis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4