title: Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro.
abstract: Recently, osteogenic precursor cells were isolated from human dental follicles, which differentiate into cementoblast- or osteoblast-like cells under in vitro conditions after the induction with dexamethasone or insulin. However, mechanisms for osteogenic differentiation are not understood in detail. In a previous study, real-time RT-PCR results demonstrated molecular mechanisms in dental follicle cells (DFCs) during osteogenic differentiation that are different from those in bone-marrow-derived mesenchymal stem cells. We analysed gene expression profiles in DFCs before and after osteogenic differentiation with the Affymetrix GeneChip(R) Human Gene 1.0 ST Array. Transcripts of 98 genes were up-regulated after differentiation. These genes could be clustered into subcategories such as cell differentiation, cell morphogenesis, and skeletal development. Osteoblast-specific transcription factors like osterix and runx2 were constitutively expressed in differentiated DFCs. In contrast, the transcription factor ZBTB16, which promotes the osteoblastic differentiation of mesenchymal stem cells as an up-stream regulator of runx2, was differentially expressed after differentiation. Transcription factors NR4A3, KLF9 and TSC22D3, involved in the regulation of cellular development, were up-regulated as well. In conclusion, we present the first transcriptome of human DFCs before and after osteogenic differentiation. This study sheds new light on the complex mechanism of osteogenic differentiation in DFCs.
authors.affiliation: Department of Operative Dentistry and Periodontology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany. christian.morsczeck@klinik.uni-regensburg.de
authors.forename: Christian
authors.initials: C
authors.lastname: Morsczeck
authors.forename: Gottfried
authors.initials: G
authors.lastname: Schmalz
authors.forename: Torsten Eugen
authors.initials: TE
authors.lastname: Reichert
authors.forename: Florian
authors.initials: F
authors.lastname: Völlner
authors.forename: Michael
authors.initials: M
authors.lastname: Saugspier
authors.forename: Sandra
authors.initials: S
authors.lastname: Viale-Bouroncle
authors.forename: Oliver
authors.initials: O
authors.lastname: Driemel
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D003795:Dental Sac / Q000166:cytology* / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D020869:Gene Expression Profiling; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009024:Morphogenesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics*; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D011987:Receptors, Steroid / Q000235:genetics; D011988:Receptors, Thyroid Hormone / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D055815:Young Adult; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D004268:DNA-Binding Proteins; D005938:Glucocorticoids; C489676:KLF9 protein, human; D051741:Kruppel-Like Transcription Factors; C490412:NR4A3 protein, human; D000075722:Promyelocytic Leukemia Zinc Finger Protein; C493709:RUNX2 protein, human; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; C404226:TSC22D3 protein, human; D014157:Transcription Factors; C092037:ZBTB16 protein, human; D003907:Dexamethasone