[Screening human keratinocyte growth factor mimic peptide with Ph.D.-7 phage display peptide library].
OBJECTIVE
To harvest human keratinocyte growth factor (KGF) mimic peptides with Ph.D.-7 phage display peptide library.


METHODS
Ph.D.-7 phage display peptide library was biopanned for 4 rounds to harvest monoclonal anti-body human KGF and then phage titer was detected. ELISA detection was performed to detect the binding force of random-selected monoclonal phages, thereafter DNA extracted from phages with better binding activity was sequenced and the Basic BLAST system was applied to conduct the sequence similarity and homology analysis.


RESULTS
After 4 rounds of biopanning, the titer of phages was increased gradually and the enrichment of specific phage mimic peptides was obtained. The titers of monoclonal phages were up to 2.0 x 10(4) pfu/mL according to ELISA detection. According to the absorbance value, the monoclonal phages with better binding activities to certain specific antibodies were sequenced, and 26 base sequences related to the promotion of division and growth were verified, 2 of which were similar to human KGF. Homology sequence analysis revealed that the common sequence of those 26 base sequences was similar to the partial sequences of human KGF.


CONCLUSIONS
The phage mimic peptides resembling or related to human KGF DNA can be harvested from Ph.D.-7 phage display peptide library, which may be conducive to improve human KGF performance, wound healing and the quality of tissue engineered skin substitutes.
Zong
Xianlei
X
Institute of Tissue Engineering of Shandong University, Jinan Shandong, 250033, P.R. China.
Cai
Jinglong
J
Pang
Li
L
Liu
Junli
J
Jiang
Duyin
D
Wang
Wei
W
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000235:genetics*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics*
D000911:Antibodies, Monoclonal; D019151:Peptide Library; D010455:Peptides; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor