Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB.
BACKGROUND
Exposure to ultraviolet (UV) radiation causes a complex cellular response, mostly mediated by the production of reactive oxygen species (ROS), which can be counteracted by exogenous treatments and endogenous mechanisms with anti-oxidant and scavenger properties. Keratinocyte growth factor (KGF/FGF7), a member of the fibroblast growth factor family, promotes epithelial growth and differentiation and is involved in cell survival after oxidant injuries.


OBJECTIVE
We analyzed the role of KGF in the control of intracellular ROS production and oxidative stress after UVB exposure on KGF receptor (KGFR) transfected cells and human immortalized and primary keratinocytes.


METHODS
We assessed the intracellular ROS production measuring the intensity of the oxidation-sensitive fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA) by confocal microscopy, as well as the catalase activity by spectrophotometric assay. Moreover, morphological and biochemical analysis of actin cytoskeleton reorganization was evaluated as a further marker of oxidative damage.


RESULTS
Our data show that KGF significantly reduces intracellular ROS generation in response to UVB, preserves the decrease of catalase activity and prevents actin cytoskeleton rearrangement.


CONCLUSIONS
Our results provide a further evidence that KGF may be crucial for an efficient skin photoprotection, demonstrating a direct role for KGF in the reduction of intracellular ROS content following UVB exposure.
Kovacs
Daniela
D
Istituto Dermatologico San Gallicano, IRCCS, Via Elio Chianesi 53, 00144 Rome, Italy.
Raffa
Salvatore
S
Flori
Enrica
E
Aspite
Nicaela
N
Briganti
Stefania
S
Cardinali
Giorgia
G
Torrisi
Maria Rosaria
MR
Picardo
Mauro
M
D000199:Actins / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D002374:Catalase / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D002460:Cell Line; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015536:Down-Regulation / Q000187:drug effects; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D041681:NIH 3T3 Cells; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000819:agonists / Q000378:metabolism*; D014162:Transfection; D014466:Ultraviolet Rays
D000199:Actins; C496453:FGF7 protein, human; D017382:Reactive Oxygen Species; D051523:Fibroblast Growth Factor 7; D002374:Catalase; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor