title: Sequence-based HLA high-resolution retyping of a bone marrow donor/recipient pair reveals the novel HLA allele DQB1*0322.
abstract: In this paper, we characterize the novel human leukocyte antigen (HLA)-DQB1*0322. We found this novel allele in a hematopoietic stem cell donor. The donor and the recipient were high-resolution HLA retyped using sequence-based typing. Both, the female patient and her donor were previously typed HLA identical, which was confirmed with the exception of the novel DQB1 allele. The novel allele is characterized by a nucleotide exchange 'G' to 'A' at position 485 in exon 3. This affected codon 130-arginine (CGG), which is replaced by glutamine (CAG) in the new allele DQB1*0322. The transplant was performed because of an acute myeloid leukemia at first remission.
authors.affiliation: Laboratory of Immunogenetics, Department of Transfusion Medicine and Haemostaseology, Clinic for Anesthesiology, University Clinic Munich, Ludwig-Maximilians-University, Munich, Germany. klaus.witter@med.uni-muenchen.de
authors.forename: K
authors.initials: K
authors.lastname: Witter
authors.forename: E
authors.initials: E
authors.lastname: Lochmann
authors.forename: C
authors.initials: C
authors.lastname: Vecchiato
authors.forename: T
authors.initials: T
authors.lastname: Albert
authors.forename: R
authors.initials: R
authors.lastname: Zahn
authors.forename: T
authors.initials: T
authors.lastname: Kauke
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001853:Bone Marrow / Q000276:immunology; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014019:Tissue Donors
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen