Signaling pathway on the effect of oxygenated warm perfusion prior to cold preservation of the liver grafts from non-heart-beating donors, and the additive effect of edaravone.
We have previously reported that oxygenated warm perfusion prior to cold preservation (preperfusion) improved the function and viability of liver grafts from non-heart-beating donors (NHBD) using an ex vivo perfusion model. In this study, we evaluated the signaling pathway underlying these effects as well as the additive effect of preperfusion administration of edaravone, a free radical scavenger. Preperfusion treatment suppressed activation of JNK, p38 MAPK, and ERK. The addition of edaravone provided an insignificant increase in bile production and a trend to a decrease in TUNEL-positive cells. Oxygenated perfusion prior to cold preservation improved the function and viability of the grafts from NHBD, which accompanied impairment of MAPK activation. Moreover, the addition of edaravone significantly enhanced the effects of preperfusion.
Kobayashi
Y
Y
Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan. yokobayashi@mail.tains.tohoku.ac.jp
Akamatsu
Y
Y
Iwane
T
T
Nakamura
A
A
Satomi
S
S
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D001646:Bile / Q000378:metabolism; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009926:Organ Preservation; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
D016166:Free Radical Scavengers; D008315:Malondialdehyde; D000410:Alanine Transaminase; D000077553:Edaravone; D000983:Antipyrine