POSTMan (POST-translational modification analysis), a software application for PTM discovery.
Post-translationally modified peptides present in low concentrations are often not selected for CID, resulting in no sequence information for these peptides. We have developed a software POSTMan (POST-translational Modification analysis) allowing post-translationally modified peptides to be targeted for fragmentation. The software aligns LC-MS runs (MS(1) data) between individual runs or within a single run and isolates pairs of peptides which differ by a user defined mass difference (post-translationally modified peptides). The method was validated for acetylated peptides and allowed an assessment of even the basal protein phosphorylation of phenylalanine hydroxylase (PHA) in intact cells.
Arntzen
Magnus Ø
MØ
PROBE Proteomic Platform, Department of Biomedicine, University of Bergen, Bergen, Norway.
Osland
Christoffer Leif
CL
Raa
Christopher Rasch-Olsen
CR
Kopperud
Reidun
R
Døskeland
Stein-Ove
SO
Lewis
Aurélia E
AE
D'Santos
Clive S
CS
D000107:Acetylation; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D010651:Phenylalanine Hydroxylase / Q000032:analysis / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012984:Software
D010455:Peptides; D010651:Phenylalanine Hydroxylase