Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples.
Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
Marinheiro
Juliana Cristina
JC
Laboratório de Biologia Molecular de Adenovírus, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil, 05508-900.
Sanalios
Roberta Braga
RB
Santos
Daniela Carvalho dos
DC
Costa
Cristovão Alves da
CA
Hársi
Charlotte Marianna
CM
D000208:Acute Disease; D000256:Adenoviridae / Q000235:genetics* / Q000302:isolation & purification; D000257:Adenoviridae Infections / Q000175:diagnosis*; D016022:Case-Control Studies; D002648:Child; D006801:Humans; D009305:Nasopharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity