First isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog.
Canine distemper caused by canine distemper virus (CDV) is a contagious, incurable, often fatal, multisystemic viral disease that affects the respiratory gastrointestinal and central nervous system. Strains Vn86 and Vn99 of CDV were isolated, we believe for the first time, in Vietnam from two 4-month-old autopsied dogs pathologically showing non-suppurative encephalitis with pneumonia, lymphoid depletion and severe gastroenteritis. These strains caused syncytium cytopathic effect in Vero cells and Vero cells expressing canine signaling lymphocyte activation molecules. The titers of cell-associated viruses of both strains were higher than for released viruses. Molecular analysis showed that both new isolates of CDV joined to the group of classic type that is far from the Asia 1 and Asia 2 groups. These results indicated that first isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog.
Lan
Nguyen Thi
NT
Department of Veterinary Pathology, Microbiology, Infectious diseases, Hanoi University of Agriculture, Trau Quy- Gialam-Hanoi, Vietnam.
Yamaguchi
Ryoji
R
Kien
Tran Trung
TT
Hirai
Takuya
T
Hidaka
Yuichi
Y
Nam
Nguyen Huu
NH
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops / Q000235:genetics; D004216:Distemper / Q000235:genetics / Q000473:pathology / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004285:Dogs; D015870:Gene Expression; D007150:Immunohistochemistry; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells; D014744:Vietnam