Aggregation chimeras: combining ES cells, diploid, and tetraploid embryos.
During the past 40 years, mouse chimeras have served as invaluable tools for studying not only genetics but also embryonic development, and the path from undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows highlighting the most commonly used as well as more recently developed applications of various cell types and origins used in the production of chimeras. Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved.
Tanaka
Mika
M
Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada.
Hadjantonakis
Anna-Katerina
AK
Vintersten
Kristina
K
Nagy
Andras
A
D000818:Animals; D002454:Cell Differentiation; D002678:Chimera / Q000502:physiology*; D004171:Diploidy; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D005260:Female; D051379:Mice; D011123:Polyploidy; D011247:Pregnancy