title: Involvement of Ca(2+) in globular adiponectin-induced reactive oxygen species.
abstract: Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. We investigated the role of Ca(2+) in gAd-induced ROS and NO generation. Pretreatment with BAPTA-AM, a selective chelator of intracellular Ca(2+) ([Ca(2+)](i)), partially reduced gAd-induced generation of ROS and NO in gAd-treated RAW 264 cells. The lowest [Ca(2+)](i) occurred 30min after gAd treatment, after which [Ca(2+)](i) increased continually and exceeded the initial level. The mitochondrial Ca(2+) ([Ca(2+)](m)) detected by Rhod-2 fluorescence started to increase at 6h after gAd treatment. Pretreatment with a NAD(P)H oxidase inhibitor, diphenyleneiodonium, prevented the reduction of [Ca(2+)](i) in the early phase after gAd treatment. Calcium depletion by BAPTA-AM had no effect on the gAd-induced [Ca(2+)](m) oscillation. The administration of a specific calmodulin inhibitor, calmidazolium, significantly suppressed gAd-induced ROS and NO generation and NOS activity.
authors.affiliation: Department of Preventive Dentistry, Kyushu University, Higashi-ku, Fukuoka, Japan. akifusa@dent.kyushu-u.ac.jp
authors.forename: Sumio
authors.initials: S
authors.lastname: Akifusa
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kamio
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimazaki
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Yamashita
mesh_terms: D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D002147:Calmodulin; D004791:Enzyme Inhibitors; D007093:Imidazoles; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C031938:calmidazolium; D002118:Calcium