title: A comprehensive analysis of immunohistochemical studies in intrahepatic cholangiocarcinoma using the survival tree model.
abstract: OBJECTIVE
  Various immunohistochemical studies have been performed regarding intrahepatic cholangiocarcinoma (ICC), including the cell cycle-related proteins (p27, cyclin D1, 14-3-3sigma, p53, cyclin B1 and Ki-67), the proto-oncogenes (c-erbB-2 and c-Met), the extracellular matrix proteins (tenascin and laminin) and others (beta-catenin, epidermal growth factor receptor, osteopontin, aquaporin 1, MUC5AC and fascin). Nevertheless, none of these have been proven to be a predictive power of the prognosis with high specificity and sensitivity for ICC.
  
  
  METHODS
  Sixty-one patients with ICC were selected and ICC specimens were immunohistochemically stained with the above 16 markers, as previously reported.
  
  
  RESULTS
  The immunoreactivity of osteopontin, tenascin and Ki-67 divided the patients with ICC into 4 subgroups by the survival tree model. There was a significant relationship between the location of the tumor, TNM classification, histological differentiation, tumor size, lymphatic permeation, perineural invasion, lymph node metastasis, intrahepatic metastasis and viral infection among the 4 subgroups. In addition, there was a significant difference in survival among the 4 subgroups.
  
  
  CONCLUSIONS
  In this study, the subgrouping by the survival tree model might be helpful for predicting the patients' prognosis in ICC.
authors.affiliation: Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. tomo@surgpath.med.kyushu-u.ac.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Iguchi
authors.forename: Natsumi
authors.initials: N
authors.lastname: Yamashita
authors.forename: Shinichi
authors.initials: S
authors.lastname: Aishima
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Terashi
authors.forename: Keishi
authors.initials: K
authors.lastname: Sugimachi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Taguchi
authors.forename: Akinobu
authors.initials: A
authors.lastname: Taketomi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Maehara
authors.forename: Masazumi
authors.initials: M
authors.lastname: Tsuneyoshi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001650:Bile Duct Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016019:Survival Analysis