[Association of TNNT2 gene mutations with idiopathic dilated cardiomyopathy in a Chengdu population].
OBJECTIVE
To determine whether cardiac troponin T gene (TNNT2, AY044273) is the virulence gene of idiopathic dilated cardiomyopathy (IDCM) in a Chengdu population.


METHODS
DNA were isolated from 96 hospitalized unrelated patients with IDCM and 110 healthy Chinese Han people who served as controls. The TNNT2 mutations including Arg131Trp, Arg141Trp, Arg205Leu, deltaLys210 and Asp270Asn were screened by polymerase chain reaction-single strand conformation polymorphisms (PCR-SSCP) and nucleotide sequence analysis.


RESULTS
No mutation (Arg141Trp, Arg131Trp, Arg205Leu, deltaLys210, Asp270Asn) in TNNT2 was found in either the IDCM patients or the healthy people. The G9005C and C13350T polymorphism of the TNNT2 appeared in both the IDCM patients and the healthy people.


CONCLUSIONS
The IDCM patients in Chengdu have no TNNT2 mutation. Two polymorphisms, G9005C and C13350T, are identified, which are not associated with IDCM.
Chen
Bin
B
Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Rao
Li
L
Zhou
Bin
B
Liu
Xing
X
Hang
Ping
P
Wu
Yang
Y
Peng
Ying
Y
Chen
Yu
Y
D001483:Base Sequence; D002311:Cardiomyopathy, Dilated / Q000235:genetics*; D002681:China; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D020107:Troponin T / Q000235:genetics*
C545568:TNNT2 protein, human; D020107:Troponin T