Glycan tagging to produce bioactive ligands for a surface plasmon resonance study via immobilization on different surfaces.
Suitable glycan derivatives will find immediate application in the study of their interactions. Here, we present an efficient synthetic strategy to introduce a fluorescent tag functionalized with an amino group into a model disaccharide structure (lactose). This strategy led to the maintenance of bioactivity, checked by the study of the interaction of this bioconjugate with a plant lectin (mistletoe lectin 1) by NMR spectroscopy, computational docking, and surface plasmon resonance (SPR). To demonstrate the versatility of this approach, we immobilized the new glycan derivatives on different surfaces, and a comparative analysis is presented and can be successfully used for biomimetic carbohydrate-protein interaction studies on the SPR biochip.
Muñoz
F Javier
FJ
Departamento de Quimica Organica y Farmaceutica, Universidad Complutense de Madrid, Spain.
Pérez
José
J
Rumbero
Angel
A
Santos
J Ignacio
JI
Cañada
F Javier
FJ
André
Sabine
S
Gabius
Hans-Joachim
HJ
Jiménez-Barbero
Jesús
J
Sinisterra
José V
JV
Hernáiz
María J
MJ
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D007785:Lactose / Q000737:chemistry; D008024:Ligands; D008968:Molecular Conformation; D037121:Plant Lectins / Q000378:metabolism; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D054788:Ribosome Inactivating Proteins / Q000378:metabolism; D054790:Ribosome Inactivating Proteins, Type 2; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D014118:Toxins, Biological / Q000378:metabolism
D005456:Fluorescent Dyes; D008024:Ligands; D037121:Plant Lectins; D011134:Polysaccharides; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488865:VAA-I protein, Viscum album; C510889:1,5-diaminonaphthalene; D015081:2-Naphthylamine; D054788:Ribosome Inactivating Proteins; D007785:Lactose