Impact of radiotherapy and chemotherapy on biomarkers of oxidative DNA damage in lung cancer patients.
OBJECTIVE
To assess oxidative damage to DNA during lung cancer (LC) treatments.


METHODS
Urinary levels of 8-oxoguanine (8-oxoGua) and levels of 8-oxo-2'-deoxyguanosine (8-oxodG) from urine and whole blood were determined in 36 non-cancer controls and 65 LC patients before any treatments. Samples were also obtained of LC patients during and after radiotherapy (RT, n=33) and chemotherapy (CT, n=16).


RESULTS
Stage IV LC patients had higher urinary 8-oxoGua and 8-oxodG levels than patients with stage I-III disease (p=0.044 and p=0.034, respectively). Urinary 8-oxodG levels increased during the first week of RT (p<0.001). Nuclear 8-oxodG increased during RT and 3 months after start of RT. Nuclear 8-oxodG levels also rose between the first two CT cycles (p=0.043), and urinary 8-oxodG levels during the sixth CT cycle (p=0.009).


CONCLUSIONS
Urinary DNA damage biomarker levels may be associated with LC stage. Both RT and CT increase the parameters of DNA oxidation.
Crohns
Marika
M
Department of Oncology, University of Tampere, P.O. Box 607, FI-33101 Tampere, Finland. macro1@sci.fi
Saarelainen
Seppo
S
Erhola
Marina
M
Alho
Hannu
H
Kellokumpu-Lehtinen
Pirkko
P
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine*; D004249:DNA Damage; D003710:Demography; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000532:radiotherapy* / Q000652:urine; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D016019:Survival Analysis; D016896:Treatment Outcome
D014408:Biomarkers, Tumor; C024829:8-hydroxyguanine; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine