title: Cytotoxicity of cardenolides and cardenolide glycosides from Asclepias curassavica.
abstract: A new cardenolide, 12beta,14beta-dihydroxy-3beta,19-epoxy-3alpha-methoxy-5alpha-card-20(22)-enolide (6), and a new doubly linked cardenolide glycoside, 12beta-hydroxycalotropin (13), together with eleven known compounds, coroglaucigenin (1), 12beta-hydroxycoroglaucigenin (2), calotropagenin (3), desglucouzarin (4), 6'-O-feruloyl-desglucouzarin (5), calotropin (7), uscharidin (8), asclepin (9), 16alpha-hydroxyasclepin (10), 16alpha-acetoxycalotropin (11), and 16alpha-acetoxyasclepin (12), were isolated from the aerial part of ornamental milkweed, Asclepias curassavica and chemically elucidated through spectral analyses. All the isolates were evaluated for their cytotoxic activity against HepG2 and Raji cell lines. The results showed that asclepin (9) had the strongest cytotoxic activity with an IC(50) value of 0.02 microM against the two cancer cell lines and the new compound 13 had significant cytotoxic activity with IC(50) values of 0.69 and 1.46 microM, respectively.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
authors.forename: Jun-Zhu
authors.initials: JZ
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Qing
authors.forename: Chang-Xiang
authors.initials: CX
authors.lastname: Chen
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Liu
mesh_terms: D031108:Asclepias / Q000737:chemistry*; D002298:Cardenolides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D002460:Cell Line; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006801:Humans
chemical_list: C541280:12,14-dihydroxy-3,19-epoxy-3-methoxy-5-card-20(22)-enolide; C541281:12-hydroxycalotropin; D002298:Cardenolides; D006027:Glycosides