Candida albicans RFX2 encodes a DNA binding protein involved in DNA damage responses, morphogenesis, and virulence.
We previously showed that Candida albicans orf19.4590, which we have renamed RFX2, expresses a protein that is reactive with antibodies in persons with candidiasis. In this study, we demonstrate that C. albicans RFX2 shares some functional redundancy with Saccharomyces cerevisiae RFX1. Complementation of an S. cerevisiae rfx1 mutant with C. albicans RFX2 partially restored UV susceptibility and the repression of DNA damage response genes. DNA damage- and UV-induced genes RAD6 and DDR48 were derepressed in a C. albicans rfx2 null mutant strain under basal conditions, and the mutant was significantly more resistant to UV irradiation, heat shock, and ethanol than wild-type strain SC5314. The rfx2 mutant was hyperfilamentous on solid media and constitutively expressed hypha-specific genes HWP1, ALS3, HYR1, ECE1, and CEK1. The mutant also demonstrated increased invasion of solid agar and significantly increased adherence to human buccal epithelial cells. During hematogenously disseminated candidiasis, mice infected with the mutant had a significantly delayed time to death compared to the wild type. During oropharyngeal candidiasis, mice infected with the mutant had significantly lower tissue burdens in the oral cavity and esophagus at 7 days and they were less likely to develop disseminated infections because of mucosal translocation. The data demonstrate that C. albicans Rfx2p regulates DNA damage responses, morphogenesis, and virulence.
Hao
Binghua
B
Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15203, USA.
Clancy
Cornelius J
CJ
Cheng
Shaoji
S
Raman
Suresh B
SB
Iczkowski
Kenneth A
KA
Nguyen
M Hong
MH
D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity* / Q000528:radiation effects; D002177:Candidiasis / Q000382:microbiology*; D004249:DNA Damage / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009154:Mutation; D014466:Ultraviolet Rays; D014774:Virulence
D004268:DNA-Binding Proteins; D005656:Fungal Proteins