title: [Effects of survivin antisense oligodeoxynucleotide on drug resistance in K562 cells].
abstract: OBJECTIVE
  To study the effect of Survivin antisense oligodeoxgnucleotide (ASODN) on drug resistance and its mechanism in K562 cells.
  
  
  METHODS
  Cells were divided into four groups: ASODN group, SODN group, Lip group and blank group. Survivin ASODN was transfered into K562 cells by liposomal reagent. The expression of Survivin mRNA was detected by RT-PCR. The sensitivity of K562 cells to adriamycin (ADM) and daunorubicin (DAM) was detected by MTT assay, while the intracellular concentration of ADM and DAM was measured by flowcytometry.
  
  
  RESULTS
  Compared to control group Survivin mRNA expression in ASODN group decreased obviously. IC50(s) of ADM and ASODN+ADM were 0.5457 mg/L and 0.1933 mg/L respectively, IC50 (s) of DAM and ASODN+DAM were 0.5408 mg/L and 0.2027 mg/L respectively. Expression of Survivin mRNA decreased by 25.8% after the transfection of ASODN. Fluorescence intensity of ADM and ASODN+ADM in K562 cells were 51.64 and 89.92, Fluorescence intensity of DAM and ASODN+DAM in K562 cells were 63.71 and 88.47.
  
  
  CONCLUSIONS
  Expression of Survivin mRNA in k562 cells was down-regulated by ASODN. Survivin ASODN is able to reverse the drug resistance via inhibition of Survivin expression and inducement of apoptosis.
authors.affiliation: Department of Hematology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yang
authors.forename: You-hua
authors.initials: YH
authors.lastname: Xu
authors.forename: Xian-hao
authors.initials: XH
authors.lastname: Wen
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D003630:Daunorubicin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D020014:K562 Cells; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D000077022:Survivin; D004317:Doxorubicin; D003630:Daunorubicin