Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall.
It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins.
Timmers
Jaap
J
CRC-Wageningen, Wageningen University and Research Centre, 6703 HD Wageningen, The Netherlands.
Vernhettes
Samantha
S
Desprez
Thierry
T
Vincken
Jean-Paul
JP
Visser
Richard G F
RG
Trindade
Luisa M
LM
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism* / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism / Q000502:physiology; D010944:Plants / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization / Q000502:physiology; D054829:RING Finger Domains / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D008565:Membrane Proteins; D010940:Plant Proteins; D005964:Glucosyltransferases; C478648:cellulose synthase