Determination of underivatized glucosamine in human plasma by high-performance liquid chromatography with electrochemical detection: application to pharmacokinetic study.
A simple, rapid and sensitive high-performance liquid chromatography method with electrochemical detection was developed for the determination of glucosamine in human plasma. Plasma samples were analyzed after a simple two-step procedure of protein precipitation with subsequent dilution. The chromatographic separation was performed on a Carbopack column (3 mm x 150 mm) with a mobile phase consisting of water and 200 mM sodium hydroxide. Detection was performed electrochemically in a pulsed voltammetry mode. The limit of detection was 2.0 ng/ml, inter- and intra-day precision were less than 10%. The method was successfully applied to the investigation of the pharmacokinetics of glucosamine in healthy man volunteers.
Pashkova
E
E
Moscow State University, Analytical Chemistry Department, 119991, GSP-1, Moscow, Russia. e_pashkova@list.ru
Pirogov
A
A
Bendryshev
A
A
Ivanaynen
E
E
Shpigun
O
O
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements; D055664:Electrochemical Techniques / Q000379:methods; D005944:Glucosamine / Q000097:blood*; D006801:Humans; D008297:Male; D015203:Reproducibility of Results
D005944:Glucosamine