title: Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis.
abstract: OBJECTIVE
  To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis.
  
  
  METHODS
  RAG-1 knockout (KO) mice, NUDE mice and microMT KO mice were compared to their wild-type controls.
  
  
  METHODS
  Mice were administered with 1 ml of Brewer's thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment.
  
  
  METHODS
  Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array.
  
  
  RESULTS
  RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, microMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines.
  
  
  CONCLUSIONS
  These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.
authors.affiliation: Phagocyte Laboratory, MRC Centre for Inflammation Research, University of Edinburgh, Edinburgh, UK.
authors.forename: T
authors.initials: T
authors.lastname: Kipari
authors.forename: S
authors.initials: S
authors.lastname: Watson
authors.forename: K
authors.initials: K
authors.lastname: Houlberg
authors.forename: S
authors.initials: S
authors.lastname: Lepage
authors.forename: J
authors.initials: J
authors.lastname: Hughes
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cailhier
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology; D018925:Chemokines / Q000276:immunology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D010537:Peritoneum / Q000166:cytology / Q000276:immunology; D010538:Peritonitis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D018925:Chemokines; D018398:Homeodomain Proteins; D015850:Interleukin-6; C064658:RAG-1 protein