Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.
Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.
Sekar
Raju
R
Department of Biological Sciences, Florida International University, Miami, Florida 33199, USA. S.Raju@Sheffield.ac.uk
Kaczmarsky
Longin T
LT
Richardson
Laurie L
LL
D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D005615:Freezing; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000379:methods*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S