title: Immunodiagnosis of bovine trypanosomiasis in Anambra and Imo states, Nigeria, using enzyme-linked immunosorbent assay: zoonotic implications to human health.
abstract: OBJECTIVE
  The prevalence of trypanosomiasis was studied in cattle, being a major source of animal protein in Nigeria, thus, a very likely means of spread of Human African Trypanosomosis (HAT).
  
  
  METHODS
  Enzyme-linked immunosorbent assay (ELISA) was used to diagnose bovine trypanosomiasis in 264 samples collected from adult cattle of mixed breeds, age and sex, in Anambra and Imo states, Nigeria.
  
  
  RESULTS
  Out of 264 samples analysed, 21 (7.96%) were seropositive for Trypanosoma congolense while 20 (7.58%) were seropositive for T. vivax and 8 (3.03%) were seropositive for T. brucei infections in both the states.
  
  
  CONCLUSIONS
  The predominant species was found to be T. congolense. Mixed infection of three species, T. vivax, T. congolense and T. brucei was found to dominate other mixed infections in both the states. ELISA detected the infection of the three species of trypanosomes in the same group of animals. The usefulness of antigen capture ELISA in the diagnosis of human or animal trypanosomiasis was established, and the possibility of the spread of HAT caused by T. brucei gambiense and T.b. rhodesiense through cattle was expressed.
authors.affiliation: Department of Immunology, College of Health Sciences, University of Ibadan, Oyo State. mikezeani@yahoo.com
authors.forename: M C
authors.initials: MC
authors.lastname: Ezeani
authors.forename: H
authors.initials: H
authors.lastname: Okoro
authors.forename: V O
authors.initials: VO
authors.lastname: Anosa
authors.forename: C C
authors.initials: CC
authors.lastname: Onyenekwe
authors.forename: S C
authors.initials: SC
authors.lastname: Meludu
authors.forename: C E
authors.initials: CE
authors.lastname: Dioka
authors.forename: C C
authors.initials: CC
authors.lastname: Azikiwe
mesh_terms: D000818:Animals; D002417:Cattle; D004197:Disease Reservoirs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009549:Nigeria; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000145:classification / Q000302:isolation & purification*; D014353:Trypanosomiasis, African / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000097:blood / Q000175:diagnosis* / Q000635:transmission; D015047:Zoonoses / Q000469:parasitology