Irradiated human chondrocytes expressing bone morphogenetic protein 2 promote healing of osteoporotic bone fracture in rats.
Bone morphogenetic protein 2 (BMP2) was selected as a transgene to regenerate osteoporotic bone defects after several BMPs were tested using a bone formation study in nude mice. Human chondrocytes were transduced with a BMP2-containing retroviral vector, and single clones were selected. The cells were characterized over numerous passages for growth and BMP2 expression. The single clones were irradiated and tested for viability. BMP2 expression lasted for 3 weeks before dying off completely after approximately 1 month. Irradiated and non-irradiated transduced chondrocytes successfully healed fractures in osteoporotic rats induced by ovariectomy. The osteoinducing effect of irradiated cells was better than that of their non-irradiated counterparts or a chondrocytes-only control. This study showed that delivering BMP2 from the transduced and irradiated chondrocytes could be an effective and safe method of repairing osteoporotic bone fractures.
Yi
Youngsuk
Y
TissueGene, Inc., Rockville, Maryland, USA.
Choi
Kyoung Baek
KB
Lim
Chae-Lyul
CL
Hyun
Jong-Pil
JP
Lee
Hyeon-Youl
HY
Lee
Kun Bok
KB
Yun
Lillian
L
Ayverdi
Asli
A
Hwang
Sally
S
Yip
Vivian
V
Noh
Moon Jong
MJ
Lee
Kwan Hee
KH
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002460:Cell Line; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D050723:Fractures, Bone / Q000628:therapy*; D005720:Gamma Rays; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D010012:Osteogenesis / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000502:physiology* / Q000528:radiation effects
D055396:Bone Morphogenetic Protein 2