title: [Genetic polymorphism of HSP70-1 gene and its correlation with resistance to mastitis in Chinese Holstein].
abstract: The polymorphisms of HSP70-1 gene in 253 Chinese Holstein dairy cows were studied, and the association between the polymorphisms and somatic cell score (SCS) were analyzed. PCR-SSCP, PCR-RFLP and DNA sequencing were used to investigate mutations in the coding region of HSP70-1 gene. The G-->A-->C mutation at 1 623 bp and G-->A mutation at 2 409 bp were found and both of them were silence mutations that caused no alteration in amino acid sequence. Chi-square test showed both loci were'nt at Hardy-Weinberg disequilibrium in Chinese Holstein. In the meanwhile, the association of 2 409 locus and SCS was not significant. However, the polymorphism at 1623 locus affected SCS significantly (P<0.05). The SCS of genotype CC was significantly lower than that of genotype AG and GG (P<0.05), so genotype CC was mastitis resistant. These results suggest that genotype CC of HSP70-1 gene may be used as a molecular and genetic marker to improve the phenotype of anti-mastitis in Chinese Holstein dairy cows.
authors.affiliation: Dairy Cattle Rearch Center, Shandong Academy of Agricultural Science, Ji'nan 250100, China. taijichi@126.com
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Cheng
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Li
authors.forename: Chang-Fa
authors.initials: CF
authors.lastname: Wang
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wang
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Li
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Sun
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Zhong
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008414:Mastitis, Bovine / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D018840:HSP70 Heat-Shock Proteins; C081884:heat-shock protein 70.1