title: Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing.
abstract: The therapeutic use of proteins has created an increasing demand for feasible and economical methods for both up- and downstream processes. However, whereas upstream processes have attracted substantial investment and commercial attention, downstream processing has been overlooked, causing a production bottleneck that is shifting the costs of production. This review focuses on the use of aqueous two-phase extraction as an option for the downstream processing of therapeutic proteins. It is a potential and promising liquid-liquid extraction technique for the purification of biomolecules, such as monoclonal antibodies, growth factors and hormones, that combines a high selectivity and biocompatibility with an easy scale-up and continuous operation mode.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Azevedo
authors.forename: Paula A J
authors.initials: PA
authors.lastname: Rosa
authors.forename: I Filipa
authors.initials: IF
authors.lastname: Ferreira
authors.forename: M Raquel
authors.initials: MR
authors.lastname: Aires-Barros
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001702:Biopharmaceutics / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D019382:Human Growth Hormone / Q000302:isolation & purification; D006801:Humans; D007328:Insulin / Q000302:isolation & purification; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; D004364:Pharmaceutical Preparations; D011108:Polymers; D019382:Human Growth Hormone