Neutrophil formylpeptide receptor single nucleotide polymorphism 348T>C in aggressive periodontitis.
BACKGROUND
Neutrophil formylpeptide receptors (FPRs) play an important role in bacterial recognition and chemotaxis. Defective FPR1 expression and impaired polymorphonuclear leukocyte chemotaxis toward bacterial formylpeptides are associated with aggressive periodontitis (AgP). The objective of this study was to determine whether single nucleotide polymorphisms (SNPs) in FPR1 are associated with AgP.


METHODS
Genomic DNA was isolated from blood samples obtained from African Americans (30 subjects with AgP and 33 healthy controls) and Turks (75 subjects with AgP and 63 healthy controls). A highly polymorphic fragment of the FPR1 gene was amplified by polymerase chain reaction and sequenced for analysis of SNPs.


RESULTS
Five previously identified SNPs were detected in African Americans, whereas six were detected in Turkish subjects. The frequency of synonymous SNP c.348T>C was significantly higher in African Americans with AgP than in controls (P = 0.029). The 348T allele was significantly associated with AgP (P = 0.010). Seven of the subjects with AgP, but none of the controls, were homozygous for 348T. FPR1 haplotypes 348T.568A, 348T.576T, and 348T.568A.576T were significantly increased in African Americans with AgP (P <0.02). The Turkish population did not exhibit significant differences in FPR1 SNP frequencies between subjects with AgP and controls.


CONCLUSIONS
FPR1 SNP c.348T>C is associated with AgP in African Americans. Individuals who are homozygous for 348T may have an increased risk for developing this disorder.
Maney
Pooja
P
Sections of Periodontology and Oral Biology, College of Dentistry, The Ohio State University Health Sciences Center, Columbus, OH 43218-2357, USA.
Emecen
Pinar
P
Mills
John S
JS
Walters
John D
JD
D000293:Adolescent; D000328:Adult; D001741:Black or African American / Q000235:genetics; D010520:Aggressive Periodontitis / Q000235:genetics*; D000483:Alleles; D003596:Cytosine; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D017622:Periodontal Attachment Loss / Q000235:genetics; D010514:Periodontal Pocket / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D044042:Receptors, Formyl Peptide / Q000235:genetics*; D012307:Risk Factors; D013941:Thymine; D014421:Turkey; D055815:Young Adult
C469986:FPR1 protein, human; D044042:Receptors, Formyl Peptide; D003596:Cytosine; D013941:Thymine