Comparison of vesicular stomatitis virus pseudotyped with the S proteins from a porcine and a human coronavirus.
The surface proteins S of severe acute respiratory syndrome coronavirus (SARS-CoV) and transmissible gastroenteritis virus (TGEV) were compared for their ability to mediate infection of viral pseudotypes based on vesicular stomatitis virus (VSV). The cell tropism of the respective pseudotypes corresponded to the tropism of the viruses from which the S protein was derived. Higher infectivity values were obtained with the SARS-CoV S protein than with the TGEV S protein. Differences were observed with respect to the importance of the cytoplasmic tail and the membrane anchor of the S proteins. In the case of the SARS-CoV S protein, truncation of the cytoplasmic tail resulted in increased infectivity. For the TGEV S protein, the inactivation of an intracellular retention signal in the cytoplasmic tail was required. Exchange of the membrane anchor of the S proteins led to a low infection efficiency. Our results indicate that related glycoproteins may show substantial differences in their ability to mediate pseudotype infection.
Schwegmann-Weßels
Christel
C
Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Glende
Jörg
J
Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Ren
Xiaofeng
X
College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, PR China.|Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
Qu
Xiuxia
X
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Deng
Hongkui
H
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Enjuanes
Luis
L
Centro Nacional de Biotecnología, CSIC, Department of Molecular and Cell Biology, Campus Universitario de Cantoblanco, Darwin 3, 28049 Madrid, Spain.
Herrler
Georg
G
Institute of Virology, University of Veterinary Medicine Hannover, Hannover, Germany.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry* / Q000235:genetics; D017384:Sequence Deletion; D064370:Spike Glycoprotein, Coronavirus; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000737:chemistry* / Q000235:genetics; D018116:Vesiculovirus / Q000235:genetics / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014774:Virulence
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus