title: Acceptor specificity and transfer efficiency of a beta-glycosidase from the Chinese white jade snail.
abstract: The acceptor specificity and transfer potential of a beta-D-glycosidase (G I), which had been purified from the China white jade snail, were further investigated by using various sugars as acceptors. G I had broad monosaccharide acceptor specificity for its transglycosylation activity. More specifically, it efficiently catalyzed the transfer of the beta-D-fucosyl, beta-D-glucosyl or beta-D-galactosyl moiety from the corresponding p-nitrophenyl-beta-D-glycopyranosides to various monosaccharides. The transfucosylation efficiency of G I was studied by using p-nitrophenyl-beta-D-fucopyranoside (pNPFuc) as the donor and glucose and xylose as the acceptors. The yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. The transfer product with glucose as the acceptor was isolated and identified as beta-fucosyl-1,6-glucose by an NMR analysis. The data from these analyses indicate that G I had broad acceptor specificity and high efficiency for transglycosylation. These uncommon properties of G I could make it a valuable biocatalyst for the synthesis of various disaccharides.
authors.affiliation: Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Luan
authors.forename: Huixin
authors.initials: H
authors.lastname: Liu
authors.forename: Guangbo
authors.initials: G
authors.lastname: Ge
authors.forename: Kun
authors.initials: K
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D002681:China; D004187:Disaccharides / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism*; D006031:Glycosylation; D012908:Snails / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004187:Disaccharides; D005959:Glucosidases