title: rs224136 on chromosome 10q21.1 and variants in PHOX2B, NCF4, and FAM92B are not major genetic risk factors for susceptibility to Crohn's disease in the German population.
abstract: OBJECTIVE
  Recently, a North American genome-wide association study identified three novel gene variants in PHOX2B, NCF4, and FAM92B as well as one single nucleotide polymorphisms (SNP; rs224136) in the intergenic region on chromosome 10q21.1 as being associated with Crohn's disease (CD). However, their influence on European CD patients as well as ulcerative colitis (UC) is unknown. Therefore we aimed to replicate these novel CD susceptibility variants in a large European cohort with inflammatory bowel disease and analyzed potential gene-gene interactions with variants in the NOD2/CARD15, IL23R, and ATG16L1 genes.
  
  
  METHODS
  Genomic DNA from 2,833 Caucasian individuals including 854 patients with CD, 476 patients with UC, and 1,503 healthy unrelated controls was analyzed for SNPs in PHOX2B (rs16853571), NCF4 (rs4821544), and FAM92B (rs8050910), including rs224136 on chromosome 10q21.1.
  
  
  RESULTS
  In our study population, no association of PHOX2B (P=0.563), NCF4 (P=0.506), FAM92B (P=0.401), and rs224136 (P=0.363) with CD was found. Similarly, none of these SNPs was associated with UC. In contrast, all analyzed SNPs in NOD2/CARD15, IL23R, and ATG16L1 were strongly associated with CD with P values ranging from 5.0x10(-3) to 1.6x10(-22), but there was no epistasis with polymorphisms in PHOX2B, NCF4, FAM92B, and rs224136.
  
  
  CONCLUSIONS
  In contrast to the North American population, PHOX2B, NCF4, FAM92B, and rs224136 are not associated with CD in the European population, whereas NOD2/CARD15, IL23R, and ATG16L1 are strongly associated with CD in both the North American and European populations, confirming these three genes as major CD susceptibility genes in Caucasian populations.
authors.affiliation: Department of Medicine II, Grosshadern, University of Munich, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Glas
authors.forename: Julia
authors.initials: J
authors.lastname: Seiderer
authors.forename: Giulia
authors.initials: G
authors.lastname: Pasciuto
authors.forename: Cornelia
authors.initials: C
authors.lastname: Tillack
authors.forename: Julia
authors.initials: J
authors.lastname: Diegelmann
authors.forename: Simone
authors.initials: S
authors.lastname: Pfennig
authors.forename: Astrid
authors.initials: A
authors.lastname: Konrad
authors.forename: Silke
authors.initials: S
authors.lastname: Schmechel
authors.forename: Martin
authors.initials: M
authors.lastname: Wetzke
authors.forename: Helga-Paula
authors.initials: HP
authors.lastname: Török
authors.forename: Johannes
authors.initials: J
authors.lastname: Stallhofer
authors.forename: Matthias
authors.initials: M
authors.lastname: Jürgens
authors.forename: Thomas
authors.initials: T
authors.lastname: Griga
authors.forename: Wolfram
authors.initials: W
authors.lastname: Klein
authors.forename: Jörg T
authors.initials: JT
authors.lastname: Epplen
authors.forename: Uwe
authors.initials: U
authors.lastname: Schiemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Mussack
authors.forename: Peter
authors.initials: P
authors.lastname: Lohse
authors.forename: Burkhard
authors.initials: B
authors.lastname: Göke
authors.forename: Thomas
authors.initials: T
authors.lastname: Ochsenkühn
authors.forename: Matthias
authors.initials: M
authors.lastname: Folwaczny
authors.forename: Bertram
authors.initials: B
authors.lastname: Müller-Myhsok
authors.forename: Stephan
authors.initials: S
authors.lastname: Brand
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000235:genetics; D003424:Crohn Disease / Q000235:genetics*; D004843:Epistasis, Genetic; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D014644:Genetic Variation; D055106:Genome-Wide Association Study; D005838:Genotype; D005858:Germany; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014157:Transcription Factors / Q000235:genetics*; D055815:Young Adult
chemical_list: D018398:Homeodomain Proteins; C109220:NBPhox protein; D014157:Transcription Factors; D019255:NADPH Oxidases; C113333:NCF4 protein, human