Enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype New Jersey.
In this study, an enzyme-linked immunosorbent assay (ELISA) using glycoprotein and a monoclonal antibody (MAb) was developed for the detection of antibodies to vesicular stomatitis virus (VSV) serotype New Jersey (NJ). The glycoprotein to be used as a diagnostic antigen was extracted from partially purified VSV-NJ, and a neutralizing MAb specific to VSV-NJ was incorporated to compete with antibodies in a blocking ELISA using glycoprotein (GP ELISA). The cutoff of the GP ELISA was set at 40% inhibition, which corresponded to a virus neutralization test (VNT) titer of 32. With this threshold, the GP ELISA exhibited 99.6% specificity for naïve sera (n = 3,005) from cattle (n = 1,040), pigs (n = 1,120), and horses (n = 845) from domestic farms. The GP ELISA did not cross-react with sera positive for foot-and-mouth disease virus, swine vesicular disease virus, or VSV serotype Indiana. The GP ELISA was more compatible with the VNT than was the nucleocapsid-based ELISA for VSV-NJ-positive sera (n = 19). Taken together, this GP ELISA could be a useful tool as an alternative to the VNT for detecting antibodies specific to VSV-NJ.
Lee
Hyang-Sim
HS
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, Republic of Korea.
Heo
Eun-Jeong
EJ
Jeoung
Hye-Young
HY
Ko
Hyo-Rim
HR
Kweon
Chang-Hee
CH
Youn
Hee-Jeong
HJ
Ko
Young-Joon
YJ
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins; D006734:Horse Diseases / Q000175:diagnosis / Q000821:virology; D006736:Horses; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology; D054260:Vesicular stomatitis New Jersey virus / Q000302:isolation & purification*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006023:Glycoproteins