Gene expression profiling of leukemia stem cells.
Characterization of gene expression programs and pathways important for normal and cancer stem cells has become an active area of investigation. Microarray analysis of various cell populations provides an opportunity to assess genomewide expression programs to define cellular identity and to potentially identify pathways activated in various stem cells. Here we describe methods to isolate a leukemia stem cell population, amplify RNA, and perform microarray analyses.
Krivtsov
Andrei V
AV
Department of Pediatric Oncology, Division of Hematology/Oncology, Children's Hospital, Dana Farber Cancer Insitute, Harvard Medical School, Boston, MA, USA.
Wang
Yingzi
Y
Feng
Zhaohui
Z
Armstrong
Scott A
SA
D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D020869:Gene Expression Profiling; D055014:Granulocyte-Macrophage Progenitor Cells / Q000378:metabolism* / Q000473:pathology; D007942:Leukemia, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism
C474558:MLL-AF9 fusion protein, human; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; D051788:Myeloid-Lymphoid Leukemia Protein