[Association of polymorphisms of N372H in BRCA2 gene and 135G/C in RAD51 gene and breast cancers].
OBJECTIVE
To determine the associations between the polymorphisms of N372H in BRCA2 gene and 135G/C in RAD51 gene and breast cancers.


METHODS
The allele and genotype frequencies of N372H in BRCA2 gene and 135G/C in RAD51 gene were analyzed with denaturing high performance liquid chromatography (DHPLC) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 71 women with breast cancers and 85 normal, women.


RESULTS
The women with breast cancers had higher frequencies of genotype HH of the N372H locus in BRCA2 gene than the normal women. No statistical difference in the polymorphic distribution of 135G/C in RAD51 gene was observed between the two groups of women.


CONCLUSIONS
The genotype HH of the N372H locus in BRCA2 is associated with breast cancers, which might be a genetic risk factor for breast cancers in Chinese populations.
Hu
Rui
R
Department of Mastologic Surgery, Sichuan Cancer Hospital, Sichuan Province, Chengdu 610041, China.
Wei
Yang
Y
Jiang
Wen-jun
WJ
Yao
Wen-xiu
WX
Long
Qi-ming
QM
Zhang
Jian-hui
JH
Liang
Ying
Y
Tang
Xiao-lan
XL
D000368:Aged; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002681:China; D005260:Female; D024522:Genes, BRCA2; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051135:Rad51 Recombinase / Q000235:genetics*
C495153:RAD51 protein, human; D051135:Rad51 Recombinase