title: START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region.
abstract: START-GAP2, also termed as DLC2, is a START domain-containing RhoGAP and a negative regulator of RhoA and Cdc42. Although it was reported as a tumor suppresser gene product, the molecular basis for function of START-GAP2 remains to be clarified. Here, we demonstrate that START-GAP2 is localized in focal adhesions through a "FAT (focal adhesion targeting)" region in the N-terminal half. START-GAP2 competes with START-GAP1/DLC1, another START domain-containing RhoGAP, in focal adhesion targeting. Moreover, the C-terminus of tensin2, one of focal adhesion components and reported to bind START-GAP1, also directly interacts with START-GAP2. These results suggest that START-GAP2 and START-GAP1 share the same molecular mechanism in targeting to focal adhesions.
authors.affiliation: Graduate School of Life Science, University of Hyogo, Harima Science Garden City, Hyogo-ken 978-1297, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kawai
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Seike
authors.forename: Takuya
authors.initials: T
authors.lastname: Iino
authors.forename: Minoru
authors.initials: M
authors.lastname: Kiyota
authors.forename: Yui
authors.initials: Y
authors.lastname: Iwamae
authors.forename: Hideo
authors.initials: H
authors.lastname: Nishitani
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yagisawa
mesh_terms: D000818:Animals; D022001:Focal Adhesions / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D000070876:Tensins; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008840:Microfilament Proteins; D010744:Phosphoric Monoester Hydrolases; D000070876:Tensins; D025521:Tumor Suppressor Proteins; C473606:Stard13 protein, mouse; C452816:TNS2 protein, human