Cerulenin analogues as inhibitors of efflux pumps in drug-resistant Candida albicans.
Overexpression of the ABC transporters Cdr1 and Cdr2 or the major facilitator Mdr1 causes multidrug resistance in the human fungal pathogen Candida albicans. The fatty acid synthesis inhibitor cerulenin and the structurally unrelated Golgi transport inhibitor brefeldin A are substrates for both types of efflux pumps in Candida albicans. In an effort to overcome efflux pump-mediated drug resistance in Candida albicans, cerulenin analogues were generated using a variety of synthesis pathways. The so obtained cerulenin derivatives were tested on multidrug-resistant Candida albicans isolates which constitutively overexpress either Mdr1 or Cdr1 and Cdr2. Some of these compounds were found to decrease Mdr1-mediated resistance to brefeldin A up to eightfold compared to the control.
Diwischek
Florian
F
Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Würzburg, Germany.
Morschhäuser
Joachim
J
Holzgrabe
Ulrike
U
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D020126:Brefeldin A / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D002569:Cerulenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D025141:Drug Resistance, Fungal / Q000187:drug effects*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors; D006801:Humans; D026901:Membrane Transport Proteins
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D000935:Antifungal Agents; C094693:CDR1 protein, Candida albicans; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D002569:Cerulenin; D020126:Brefeldin A