Identification and antifungal assay of a wheat beta-1,3-glucanase.
A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
Liu
Baoye
B
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Lu
Yan
Y
Xin
Zhiyong
Z
Zhang
Zengyan
Z
D000528:Alternaria / Q000187:drug effects*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D043326:Glucan 1,3-beta-Glucosidase / Q000302:isolation & purification / Q000494:pharmacology*; D010838:Phytophthora / Q000187:drug effects*; D012232:Rhizoctonia / Q000187:drug effects*; D014908:Triticum / Q000201:enzymology*
D000935:Antifungal Agents; D043326:Glucan 1,3-beta-Glucosidase