title: Neuroprotective effects of Kangen-karyu on spatial memory impairment in an 8-arm radial maze and neuronal death in the hippocampal CA1 region induced by repeated cerebral ischemia in rats.
abstract: In the present study, we investigated the neuroprotective effects of Kangen-karyu (KGK) in a repeated cerebral ischemia model (2 x 10 min, 1-h interval). A 21-day pre- and post-ischemic treatment with KGK (10 - 300 mg/kg) and aspirin (5 mg/kg) improved the spatial memory impairment and neuronal death in the hippocampal CA1 region induced by repeated cerebral ischemia. However, a 7-day post-ischemic treatment with KGK did not attenuate the spatial memory impairment and neuronal death in this model. To determine the mechanism of action of KGK, we investigated the effects of a 14-day pre-ischemic treatment with KGK on cerebral blood flow in the hippocampal area of the repeated cerebral ischemia model using laser Doppler flowmetry. The 14-day pre-ischemic treatment with KGK increased the cerebral blood flow during reperfusion. These results suggest that a 21-day pre- and post-ischemic treatment with KGK can protect against brain damage caused by cerebral ischemia by increasing the cerebral blood flow in the hippocampal area.
authors.affiliation: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
authors.forename: Fengling
authors.initials: F
authors.lastname: Pu
authors.forename: Kyoko
authors.initials: K
authors.lastname: Motohashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kaneko
authors.forename: Yurika
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Naomi
authors.initials: N
authors.lastname: Manome
authors.forename: Keiichi
authors.initials: K
authors.lastname: Irie
authors.forename: Jirou
authors.initials: J
authors.lastname: Takata
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Egashira
authors.forename: Ryozo
authors.initials: R
authors.lastname: Oishi
authors.forename: Takuya
authors.initials: T
authors.lastname: Okamoto
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sei
authors.forename: Takako
authors.initials: T
authors.lastname: Yokozawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Mishima
authors.forename: Katsunori
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Michihiro
authors.initials: M
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D006624:Hippocampus / Q000098:blood supply / Q000187:drug effects; D017078:Laser-Doppler Flowmetry; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000188:drug therapy* / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; C468270:Kangen-karyu; D018696:Neuroprotective Agents; D001241:Aspirin