title: Association between human leukocyte antigen class II alleles and human papillomavirus-mediated cervical cancer in Indian women.
abstract: We investigated the association of human leukocyte antigen (HLA) II (DRB1 and DQB1) alleles with susceptibility to human papillomavirus (HPV)-associated cervical precancer and cancer cases in a hospital-based case-control study in a northern Indian population. A total of 202 subjects, including 100 patients comprising 31 cervical precancer (cervical intraepithelial neoplasia [CIN] 2/3) and 69 invasive cervical cancer cases, and 102 healthy controls participated in the study. Both patients and controls were screened for HPV infection using a polymerase chain reaction (PCR-based approach. Low-resolution PCR-sequence specific priming (PCR-SSP) was used to genotype HLA II (DRB1 and DQB1). Our results demonstrate that the DRB1*15 allele/DRB1*15-DQB1*06 haplotype may have a predisposition for HPV infection (p(c) < 0.05) or cervical cancer/precancer (p(c) < 0.05) development, whereas the DRB1*04 allele/DRB1*04-DQB1*03 haplotype might exhibit susceptibility to cervical precancerous lesions (p(c) < 0.05). The DRB1*13 allele/DRB1*13-DQB1*06 haplotype was strongly protective against risk to HPV infection (p(c) < 0.002) as well as cervical cancer (p(c) 0.01). Therefore, we have demonstrated that HLA DR-DQ polymorphisms are involved in genetic susceptibility to cervical cancer or HPV infection in a northern Indian population.
authors.affiliation: Division of Molecular Genetics and Biochemistry, Institute of Cytology and Preventive Oncology (ICMR), I-7, Sector 39, Noida 201 301, India.
authors.forename: Indu
authors.initials: I
authors.lastname: Kohaar
authors.forename: Showket
authors.initials: S
authors.lastname: Hussain
authors.forename: Nisha
authors.initials: N
authors.lastname: Thakur
authors.forename: Pratibha
authors.initials: P
authors.lastname: Tiwari
authors.forename: Vilas
authors.initials: V
authors.lastname: Nasare
authors.forename: Swaraj
authors.initials: S
authors.lastname: Batra
authors.forename: Veena
authors.initials: V
authors.lastname: Singh
authors.forename: Suresh
authors.initials: S
authors.lastname: Bhambani
authors.forename: Bhudev C
authors.initials: BC
authors.lastname: Das
authors.forename: Debi P
authors.initials: DP
authors.lastname: Sarkar
authors.forename: Mausumi
authors.initials: M
authors.lastname: Bharadwaj
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D007194:India; D015810:Linkage Disequilibrium; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000235:genetics*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000235:genetics*
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains