[Effects of circadian gene mPer1 on therapeutic sensitivity of adriamycin].
OBJECTIVE
To study the effects of mPer1 gene on the response of mammary carcinoma EMT6 cells to Adriamycin in vitro.


METHODS
The eukaryotic expression vector pcDNA3. 1 (+)-mPer1 was transfected into the EMT6 cells (EMT6-mPerl). The vector pcDNA3. 1(+) transfect was also performed to serve as the control (EMT6-vect). The transfect efficiency was detected by RT-PCR and Western Blotting. The transfect cells were treated with Adriamycin in vitro. The apoptosis and distribution of cells in the cell cycle were analysed by FCM. The cell proliferation was detected by MTT assay. RT-PCR was used to show the mRNA expression of apoptosis-related genes.


RESULTS
The mPerl-transfected EMT6 cells revealed S phase arrest, increased rate of apoptosis [EMT6-vect: (65.65 +/- 0.07)%; EMT6-mPer1: (72.35 +/- 0.57)%], decreased cell proliferation CEMT6-vect: (42.18 +/- 5.73)%; EMT6-mPer1: (53.28 +/- 7.32%)%] and stronger expression of p53 mRNA in RT-PCR (EMT6-vect, 0.48 +/- 0.08; EMT6-mPer1: 1.18 +/- 0.02).


CONCLUSIONS
mPer1 gene can improve the drug sensitivity of this cell line to ADM in vitro.
Duan
Zhi-qing
ZQ
Department of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhu
Xiao-yun
XY
Zhang
Yu
Y
Wang
Yu-hui
YH
Liu
Yan-you
YY
Guo
Hui-ling
HL
Xiao
Jing
J
Wang
Zheng-rong
ZR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D004357:Drug Synergism; D005822:Genetic Vectors; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D056950:Period Circadian Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin