Review paper: Gene expression profiling in veterinary and human medicine: overview of applications and proposed quality control practices.
High throughput molecular analysis of veterinary tissue samples is being applied to a wide range of research questions aimed at improving survival, development of diagnostic assays, and improving the economics of commercial production of animal products. Many of these efforts also, implicitly or explicitly, have ramifications for the clinical care of humans and, potentially, animals. Here we provide an overview of applications of gene expression profiling in veterinary research and practice. We then focus on the current state of quality control and quality assurance efforts in gene expression profiling studies, underscoring lessons learned from such analysis of human samples. Finally, we propose practices aimed at ensuring the reliability and reproducibility of such assays. The implementation of quality assurance practices by a trained pathologist is an essential link in the chain of events leading ultimately to reliable and reproducible research findings and appropriate clinical care.
Kort
E J
EJ
Laboratory of Molecular Epidemiology, Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503, USA.
Norton
P
P
Haak
P
P
Berghuis
B
B
Ramirez
S
S
Resau
J
J
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards* / Q000662:veterinary*; D006801:Humans; D011786:Quality Control; D014730:Veterinary Medicine / Q000379:methods*