Structural characterization of an oligosaccharide made by Neisseria sicca.
Neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. Using matrix-assisted laser desorption ionization--time of flight and electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. Gas chromatography identified the sugars composing the unit as rhamnose and N-acetyl-D-glucosamine. Glycosidase digestion confirmed the identity of the nonreducing terminal sugar of the disaccharide and established its beta-anomeric configuration. Mass spectrometry analysis and lectin binding were used to verify the linkages within the disaccharide repeat. The results revealed that the disaccharide repeat is [-4) beta-L-rhamnose (1-3) beta-N-acetyl-D-glucosamine (1-] with an N-acetyl-D-glucosamine nonreducing terminus. This work is the first structural characterization of a molecule that possesses rhamnose in the genus Neisseria.
O'Connor
Ellen T
ET
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Zhou
Hui
H
Bullock
Kevin
K
Swanson
Karen V
KV
Griffiss
J McLeod
JM
Reinhold
Vernon N
VN
Miller
Clinton J
CJ
Stein
Daniel C
DC
D000117:Acetylglucosamine / Q000737:chemistry; D015153:Blotting, Western; D002849:Chromatography, Gas; D004187:Disaccharides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D042808:Neisseria sicca / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D012210:Rhamnose / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004187:Disaccharides; D009844:Oligosaccharides; D012210:Rhamnose; D000117:Acetylglucosamine