An alternative and fast method for determination of glyphosate and aminomethylphosphonic acid (AMPA) residues in soybean using liquid chromatography coupled with tandem mass spectrometry.
A simple and specific method using reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was investigated, which allowed the determination of residues of glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), in soybean samples. An aqueous extraction with liquid-liquid partition followed by protein precipitation was performed before the LC/MS/MS determination. The quantitation of glyphosate and AMPA was performed in positive and negative ESI mode, respectively, using the multiple reaction monitoring (MRM) mode with three transitions for each analyte to enhance the specificity of the method and avoid false positives. The methodology reported in this work is capable of detecting residues of glyphosate and AMPA in soybean samples with limits of quantification of 0.30 and 0.34 mg kg(-1), respectively. This alternative method has throughput advantages such as simpler sample preparation and faster chromatographic analysis.
Martins-Júnior
Helio A
HA
Instituto de Pesquisas Energéticas e Nucleares, Av. Prof. Lineu Prestes 2242, Cidade Universitária, São Paulo, Brazil.
Lebre
Daniel T
DT
Wang
Alexandre Y
AY
Pires
Maria A F
MA
Bustillos
Oscar V
OV
D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D006540:Herbicides / Q000032:analysis*; D007555:Isoxazoles; D063065:Organophosphonates / Q000032:analysis*; D015203:Reproducibility of Results; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013777:Tetrazoles; D000097797:Glyphosate
D005998:Glycine; D006540:Herbicides; D007555:Isoxazoles; D063065:Organophosphonates; D013777:Tetrazoles; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid