title: Crystallization and preliminary X-ray analysis of a cohesin-like module from AF2375 of the archaeon Archaeoglobus fulgidus.
abstract: A cohesin-like module of 160 amino-acid residues from the hypothetical protein AF2375 of the noncellulolytic, hyperthermophilic, sulfate-reducing archaeon Archaeoglobus fulgidus was cloned, expressed, purified, crystallized and subjected to X-ray structural study in order to compare its structure with those of cellulolytic cohesins. The crystals had cubic symmetry, with unit-cell parameters a = b = c = 101.75 A in space group P4(3)32, and diffracted to 1.82 A resolution. The asymmetric unit contained a single cohesin molecule. A model assembled from six cohesin structures (PDB entries 1anu, 1aoh, 1g1k, 1qzn, 1zv9 and 1tyj) of very low sequence identity to the cohesin-like module was used in molecular-replacement attempts, producing a marginal solution.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Israel.
authors.forename: Milana
authors.initials: M
authors.lastname: Voronov-Goldman
authors.forename: Ilit
authors.initials: I
authors.lastname: Noach
authors.forename: Raphael
authors.initials: R
authors.lastname: Lamed
authors.forename: Linda J W
authors.initials: LJ
authors.lastname: Shimon
authors.forename: Ilya
authors.initials: I
authors.lastname: Borovok
authors.forename: Edward A
authors.initials: EA
authors.lastname: Bayer
authors.forename: Felix
authors.initials: F
authors.lastname: Frolow
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D019611:Archaeoglobus fulgidus / Q000737:chemistry*; D018797:Cell Cycle Proteins / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D000097722:Cohesins
chemical_list: D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins