title: HPV genotyping from invasive cervical cancer in Chile.
abstract: OBJECTIVE
  To determine the prevalence rates of the different HPV types in cervical cancer lesions in Chile to facilitate the development of prophylactic human papillomavirus (HPV) vaccines effective for that country.
  
  
  METHODS
  Biopsy samples of 312 cervical cancer lesions were assessed for HPV type by reverse-line blotting assay.
  
  
  RESULTS
  HPV DNA was found in 94.2% of the lesions, 67.2% harboring 1 viral type and the remainder harboring more than 1 type. HPV-16 was the most frequent type in single infections (50.5%), followed by HPV-18 (7.8%), HPV-31 (2.4%), and HPV-45 (2.0%). HPV-16 was also present in 98.7% of dual and multiple infections, its most frequent association being with HPV-18.
  
  
  CONCLUSIONS
  HPV types 16, 18, 31, and 45, alone or combined with other types, were observed in the biopsy samples of up to 80.5% of cervical cancer lesions.
authors.affiliation: Molecular Pathology Laboratory, Department of Pathology, Universidad de la Frontera School of Medicine, Temuco, Chile.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Roa
authors.forename: Patricia
authors.initials: P
authors.lastname: Garcia
authors.forename: Jorge
authors.initials: J
authors.lastname: Gomez
authors.forename: Wanda
authors.initials: W
authors.lastname: Fernández
authors.forename: Fancy
authors.initials: F
authors.lastname: Gaete
authors.forename: Arturo
authors.initials: A
authors.lastname: Espinoza
authors.forename: Alejandro
authors.initials: A
authors.lastname: Lepetic
authors.forename: Eugenio
authors.initials: E
authors.lastname: Suarez
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000821:virology*; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology*; D002677:Chile / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral