title: Effects of chelating reagents on colonial appearance of Paenibacillus alvei isolated from canine oral cavity.
abstract: A bacterial strain isolated from the oral cavity of a healthy dog revealed an unusual colony formation in nebular appearance on agar plates. The isolated bacterial strain was Gram-positive, spore-forming rod with peritrichous flagella, and grown under aerobic conditions, but unable to grow at 45 degrees C. The strain was tentatively classified as Paenibacillus alvei according to the biochemical properties and the 16S rRNA gene sequence. The isolate exhibits collective locomotion on solid agar plates. The bacterial motility was inhibited with EDTA and was restored by adding magnesium. We concluded that magnesium ion is essential for collective locomotion of P. alvei. This suggests that EDTA is useful for inhibition of biofilm formation.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Ueda, Morioka, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujihara
authors.forename: Kouji
authors.initials: K
authors.lastname: Maeda
authors.forename: Eriko
authors.initials: E
authors.lastname: Sasamori
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Matsushita
authors.forename: Ryô
authors.initials: R
authors.lastname: Harasawa
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis*; D004285:Dogs / Q000382:microbiology*; D004492:Edetic Acid / Q000494:pharmacology; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008274:Magnesium / Q000502:physiology; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics
chemical_list: D002614:Chelating Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004492:Edetic Acid; D008274:Magnesium