Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle.
OBJECTIVE
The aim of this study was to investigate the type and the nature of peptides present in the in vivo formed human acquired enamel pellicle.


METHODS
Pellicle material was collected from 10 volunteers and subjected to sample preparations consisting of centrifugal filtration using a 10 kDa molecular weight cut-off membrane and high-resolution gel filtration chromatography. The fractions containing peptides <10 kDa obtained by both methods were analyzed by LC-ESI-MS/MS.


RESULTS
78 natural pellicle peptides with molecular weights ranging from 766.9 Da to 3981.4 Da were identified originating from 29 different proteins.


CONCLUSIONS
The number of peptides present in acquired enamel pellicle appears to be large and this is likely to enhance the functional spectrum of this protein film. The presence of small peptides in pellicle may be functionally important since structure/function studies of many salivary proteins have shown that specific domains within these native proteins retain or even exhibit enhanced biological activities. The data present the basis for determining the precise function of these pellicle peptides and for gaining insights into the role pellicle plays in the oral cavity.
Siqueira
Walter L
WL
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston University, 700 Albany Street, CABR, W201, Boston, MA 02118, United States.
Oppenheim
Frank G
FG
D000328:Adult; D017305:Annexin A1 / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D055312:Cystatin A / Q000032:analysis; D044622:Dental Pellicle / Q000737:chemistry*; D005260:Female; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D008851:Micropore Filters; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis; D011480:Protease Inhibitors / Q000032:analysis; D020543:Proteome / Q000032:analysis; D055333:Salivary Cystatins / Q000032:analysis; D055273:Salivary Proline-Rich Proteins / Q000032:analysis; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D055291:Salivary alpha-Amylases / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
D017305:Annexin A1; D002135:Calcium-Binding Proteins; D055312:Cystatin A; D010750:Phosphoproteins; D011480:Protease Inhibitors; D020543:Proteome; C462950:S100A14 protein, human; C018149:STATH protein, human; D055333:Salivary Cystatins; D055273:Salivary Proline-Rich Proteins; D012471:Salivary Proteins and Peptides; C026407:CSTA protein, human; D055291:Salivary alpha-Amylases