[Cell death in Jurkat cells induced by oxygen/nitrogen stress].
The maintenance of balance between lymphocyte proliferation and lymphocyte death is extremely important for normal functioning of the immune system. Considering essential role of nitrogen and oxygen radicals in functioning of immune competent cells, we studied the mechanisms of cell death induced by nitrogen/oxygen stress on the model of Jurkat cell line. We have observed that hyperproduction of reactive oxygen in Jurkat cells incubated with hydrogen peroxide contributes to the activation of membrane peroxidation, to a decrease in the intensity of apoptosis and its replacement by more severe mechanism of cell death - necrosis, which is obviously conditioned by a dramatic decrease in the intensity of energogenesis in mitochondria. In cells incubated with sodium nitroprusside moderate NO-induced inhibition of electron transport in oxidative chain and mitochondrial energogenesis and intensification of oxidative stress in Jurkat cells is accompanied with the activation of the cell death mechanisms- both apoptosis and necrosis.
Enukidze
M G
MG
Machavariani
M G
MG
Intskirveli
N A
NA
Bezhitashvili
N D
ND
Sanikidze
T V
TV
D016923:Cell Death / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D009584:Nitrogen / Q000009:adverse effects* / Q000493:pharmacokinetics; D018384:Oxidative Stress / Q000502:physiology; D010100:Oxygen / Q000009:adverse effects* / Q000493:pharmacokinetics
D009569:Nitric Oxide; D009584:Nitrogen; D010100:Oxygen