title: [Tissue distribution of solid lipid nanoparticles loaded garlic oil in rats].
abstract: OBJECTIVE
  To investigate the tissue distribution of the diallyl disulfide (DADS) and diallyl trisulfide (DATS) in solid lipid nanoparticles loaded garlic oil (GO-SLN) in rats.
  
  
  METHODS
  The gas chromatography-electron capture detection (GC-ECD) method was established to determined the DADS and DATS simultaneously in the biological samples of rats after administration of 0.5 mL garlic oil injection or GO-SLN (containing about 10 mg garlic oil) via jugular vein cannula. The conditions for gas chromatographic separation were as follows. The oven temperature was set at 110 degrees C and maintained for 15 min. Temperatures at the injection port and detector were 180 degrees C and 300 degrees C, respectively. Ultra-pure nitrogen (purity > 99.999%, Shenyang Kerui Special Gases Co. Ltd., China) was used as a carrier gas and made-up gas at flow-rates of 1 mL x min(-1) and 60 mL x min(-1), respectively. All injections were carried out in the split injection mode with a split ratio of 1:10.
  
  
  RESULTS
  The GC-ECD method was fit for determing the concentration of DADS and DATS in garlic oil. The distribution character of GO-SLN in rats had changed to some extent and the concentration of GO-SLN in tissues was higher than that of GO-Injection.
  
  
  CONCLUSIONS
  The SLN can elevate the passive targeting of drugs and lengthen their action time in tissues.
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Region, Shenyang 110016, China. sunxh4545@163.com
authors.forename: Xue-hui
authors.initials: XH
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Ming-hong
authors.initials: MH
authors.lastname: Zhao
authors.forename: Shu-fang
authors.initials: SF
authors.lastname: Nie
mesh_terms: D000498:Allyl Compounds / Q000032:analysis / Q000493:pharmacokinetics*; D000818:Animals; D004220:Disulfides / Q000032:analysis / Q000493:pharmacokinetics*; D005260:Female; D005737:Garlic / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D000498:Allyl Compounds; D004220:Disulfides; D010938:Plant Oils; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide