title: Comparison of UV and charged aerosol detection approach in pharmaceutical analysis of statins.
abstract: CAD (charged aerosol detector) has recently become a new alternative detection system in HPLC. This detection approach was applied in a new HPLC method for the determination of three of the major statins used in clinical treatment-simvastatin, lovastatin and atorvastatin. The method was optimized and the influence of individual parameters on CAD response and sensitivity was carefully studied. Chromatography was performed on a Zorbax Eclipse XDB C18 (4.6 mm x 75 mm, 3.5 microm), using acetonitrile and formic acid 0.1% as mobile phase. The detection was performed using both CAD (20 pA range) and DAD (diode array detector-238 nm) simultaneously connected in series. In terms of linearity, precision and accuracy, the method was validated using tablets containing atorvastatin and simvastatin. The CAD is designated to be a non-linear detector in a wide dynamic range, however, in this application and in the tested concentration range its response was found to be perfectly linear. The limits of quantitation (0.1 microg/ml) were found to be two times lower than those of UV detection.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. novakoval@faf.cuni.cz
authors.forename: Lucie
authors.initials: L
authors.lastname: Nováková
authors.forename: Sofía Arnal
authors.initials: SA
authors.lastname: Lopéz
authors.forename: Dagmar
authors.initials: D
authors.lastname: Solichová
authors.forename: Dalibor
authors.initials: D
authors.lastname: Satínský
authors.forename: Bohumila
authors.initials: B
authors.lastname: Kulichová
authors.forename: Ales
authors.initials: A
authors.lastname: Horna
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
mesh_terms: D000336:Aerosols; D000069059:Atorvastatin; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004864:Equipment and Supplies; D006538:Heptanoic Acids / Q000032:analysis; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000032:analysis*; D008148:Lovastatin / Q000032:analysis; D011758:Pyrroles / Q000032:analysis; D019821:Simvastatin / Q000032:analysis; D013607:Tablets / Q000032:analysis
chemical_list: D000336:Aerosols; D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011758:Pyrroles; D013607:Tablets; D008148:Lovastatin; D000069059:Atorvastatin; D019821:Simvastatin