title: [Histological evaluation of hepatic fibrosis in patients with post surgical bile duct injury].
abstract: BACKGROUND
  Bile duct injuries after cholecystectomy can produce fibrous and collagen deposit tissue. Our objective was to evaluate the liver fibrosis measured in histological tissue in patients with bile duct injury after cholecystectomy.
  
  
  METHODS
  Three normal liver biopsies and 21 from patients with bile duct injuries were studied. Group I: with three normal liver biopsies. Group II: with external abdominal fistula alone in six patients. Group III with complete bile duct obstruction in 15 patients. The surgical biliary enteric reconstructions were performed 8 weeks after bile duct injury in all cases. The fibrosis and collagen deposits were studied by Masson's trichrome and Sirius red stains and they were measured by a digital program.
  
  
  RESULTS
  Group I showed 2 % of fibrosis tissue and 1% of collagen deposit and was considered as normal. Group II showed unexpected 1 fold more liver fibrosis and 9 fold more collagen deposit in extracellular matrix macromolecule (p < 0.05, Anova) against group I. Patients in group III, had fibrous tissue increase 43 folds more and 14 collagen folds more (p < 0.0001, Bonferroni's post hoc) versus group I.
  
  
  CONCLUSIONS
  The patients in groups II and III showed liver fibrosis, being this more important in group III.
authors.affiliation: Departamento de Cirugía General, Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social (CMNO IMSS), Guadalajara, Jalisco, México.
authors.forename: José M
authors.initials: JM
authors.lastname: Hermosillo-Sandoval
authors.forename: Vicente
authors.initials: V
authors.lastname: Ramírez-Casillas
authors.forename: Sebastián
authors.initials: S
authors.lastname: Hernández-Hoyos
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Osuna-Rubio
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Nuño-Guzmán
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Gálvez-Gastélum
authors.forename: Alejandra G
authors.initials: AG
authors.lastname: Miranda-Díaz
mesh_terms: D000328:Adult; D001652:Bile Ducts / Q000293:injuries*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007431:Intraoperative Complications; D008103:Liver Cirrhosis / Q000209:etiology* / Q000473:pathology*; D008297:Male