One-step construction of reagentless biosensor based on chitosan-carbon nanotubes-nile blue-horseradish peroxidase biocomposite formed by electrodeposition.
A simple and controllable electrodeposition approach was established for one-step construction of novel reagentless biosensors by in situ formation of chitosan-carbon nanotubes-nile blue-horseradish peroxidase (CS-CNTs-NB-HRP) biocomposite film on electrode surface. The mediator effect of NB, conducting performance of CNTs and the biocompatible microenvironment of CS were combined by such one-step non-manual process. NB could interact with CNTs and resulted in good dispersion of CNTs-NB nanocomposites in aqueous solution. Cyclic voltammetry measurements demonstrated that electrons were efficiently shuttled between HRP and the electrode mediated by NB. The developed reagentless biosensor exhibited a fast amperometric response for the determination of H(2)O(2) and 95% of the steady-state current was obtained within 2s. The linear response of the reagentless biosensor for the determination of H(2)O(2) ranged from 1.0 x 10(-6) to 2.4 x 10(-4)mol l(-1) with a detection limit of 1.2 x 10(-7)mol l(-1). The biosensor exhibited high reproducibility and long-time storage stability. The as-prepared biosensor also showed effective anti-interference capability. The ease of the one-step non-manual technique and the promising feature of the biocomposite could serve as a versatile platform for fabricating electrochemical biosensors.
Xi
Fengna
F
Department of Chemistry, Zhejiang University, Hangzhou 31002, PR China.
Liu
Lijun
L
Chen
Zhichun
Z
Lin
Xianfu
X
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D048271:Chitosan; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis; D037742:Nanotubes, Carbon; D010078:Oxazines
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D010078:Oxazines; C008619:Nile Blue; D048271:Chitosan; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase