Highly specific detection of interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor dip-probe.
We are reporting highly specific and sensitive detection of human interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor (CTFOB) dip-probe. With these probes we could successfully detect IL-6, down to a concentration of 5 pM (0.12 ng/ml) in the presence of much higher concentration of a non specific protein. Sandwich immunoassay was used to generate specific fluorescence signal. A novel strategy is developed to eliminate the false signal from non specific binding. In this new strategy it is not required to pre-treat probe surface with any kind of blocking buffer. The specificity of the sensor was established by incubating negative control probes in high concentration (1 nM) of another cytokine IL-8.
Kapoor
Rakesh
R
Department of Physics, University of Alabama at Birmingham, Birmingham, AL 35294, United States. rkapoor@uab.edu
Wang
Chun-Wei
CW
D015374:Biosensing Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D015850:Interleukin-6 / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation*
D015850:Interleukin-6