title: Andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome.
abstract: Hantavirus cardiopulmonary syndrome (HCPS) is a highly pathogenic emerging disease (40% case fatality rate) caused by New World hantaviruses. Hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. At present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hospital admission and aggressive pulmonary and hemodynamic support. In this study, we show that Andes virus (ANDV) interacts with human apolipoprotein H (ApoH) and that ApoH-coated magnetic beads or ApoH-coated enzyme-linked immunosorbent assay plates can be used to capture and concentrate the virus from complex biological mixtures, such as serum and urine, allowing it to be detected by both immunological and molecular approaches. In addition, we report that ANDV-antigens and infectious virus are shed in urine of HCPS patients.
authors.affiliation: Laboratorio de Infectología y Virología Molecular, Centro de Investigaciones Médicas y Departamento de Pediatría, Facultad de Medicina, Pontificia Universidad Católica de Chile, Marcoleta 391, Santiago, Chile.
authors.forename: Paula
authors.initials: P
authors.lastname: Godoy
authors.forename: Delphine
authors.initials: D
authors.lastname: Marsac
authors.forename: Elias
authors.initials: E
authors.lastname: Stefas
authors.forename: Pablo
authors.initials: P
authors.lastname: Ferrer
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Tischler
authors.forename: Karla
authors.initials: K
authors.lastname: Pino
authors.forename: Pablo
authors.initials: P
authors.lastname: Ramdohr
authors.forename: Pablo
authors.initials: P
authors.lastname: Vial
authors.forename: Pablo D T
authors.initials: PD
authors.lastname: Valenzuela
authors.forename: Marcela
authors.initials: M
authors.lastname: Ferrés
authors.forename: Francisco
authors.initials: F
authors.lastname: Veas
authors.forename: Marcelo
authors.initials: M
authors.lastname: López-Lastra
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology / Q000652:urine*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D006476:Orthohantavirus / Q000276:immunology*; D018804:Hantavirus Pulmonary Syndrome / Q000097:blood / Q000276:immunology / Q000652:urine*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008863:Microspheres; D012367:RNA, Viral / Q000032:analysis; D014709:Vero Cells; D053482:beta 2-Glycoprotein I / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral; D053482:beta 2-Glycoprotein I