Significance of measuring serum concentrations of remnant lipoproteins and apolipoprotein B-48 in fasting period.
OBJECTIVE
To characterize lipid profiles conveniently in the fasting period to detect postprandial hyperlipidemic subjects, we measured the concentrations of lipids, including remnant lipoproteins and apoB-48, before and after loading the test meal in 24 normolipidemic subjects.


METHODS
We examined remnant-like particle-cholesterol and -triglyceride (RLP-C, RLP-TG) by the immune adsorption method, RemL-C by the newly developed homogeneous method, and apoB-48 by chemiluminescence enzyme immunoassay.


RESULTS
After loading, TG, RemL-C, RLP-C, RLP-TG, and apoB-48 concentrations were elevated. Twenty subjects had only a slight elevation of TG (low TG group) after loading, while 4 subjects showed apparent increase of TG (more than 150 mg/dL, high TG group). In the fasting period, the high TG group had significantly higher serum concentrations of TG and RemL-C than the low TG group. Although not significant, RLP-C, RLP-TG and apoB-48 concentrations in the high TG group were also higher than in the low TG group. After loading, serum concentrations of TG, RemL-C, RLP-C, RLP-TG, and apoB-48 increased significantly more in the high TG group than in the low TG group.


CONCLUSIONS
In conclusion, TG, RemL-C, RLP-C, RLP-TG, and apoB-48 concentrations in the fasting period may be suitable for detecting postprandial hyperlipidemic subjects.
Sato
Itsuko
I
Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan.
Ishikawa
Yuichi
Y
Ishimoto
Ai
A
Katsura
Shiho
S
Toyokawa
Atsushi
A
Hayashi
Fujio
F
Kawano
Seiji
S
Fujioka
Yoshio
Y
Yamashita
Shizuya
S
Kumagai
Shunichi
S
D053283:Apolipoprotein B-48 / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D004435:Eating; D005215:Fasting; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
D053283:Apolipoprotein B-48; D008074:Lipoproteins; D014280:Triglycerides; C112017:remnant-like particle cholesterol; D002784:Cholesterol