title: Assessment of genetic damage in peripheral blood of human volunteers exposed (whole-body) to a 200 muT, 60 Hz magnetic field.
abstract: OBJECTIVE
  To investigate the extent of damage in nucleated cells in peripheral blood of healthy human volunteers exposed to a whole-body 60 Hz, 200 microT magnetic field.
  
  
  METHODS
  In this study, 10 male and 10 female healthy human volunteers received a 4 h whole-body exposure to a 200 microT, 60 Hz magnetic field. In addition, five males and five females were treated in a similar fashion, but were exposed to sham conditions. For each subject, a blood sample was obtained prior to the exposure period and aliquots were used as negative- (pre-exposure) and positive- [1.5 Gray (Gy) (60)Cobalt ((60)Co) gamma-irradiation] controls. At the end of the 4 h exposure period, a second blood sample was obtained. The extent of DNA damage was assessed in peripheral human blood leukocytes from all samples using the alkaline comet assay. To detect possible clastogenic effects, the incidence of micronuclei was assessed in phytohemagglutinin (PHA)-stimulated lymphocytes using the cytokinesis-block micronucleus assay.
  
  
  RESULTS
  There was no evidence of either increased DNA damage, as indicated by the alkaline comet assay, or increased incidence of micronuclei (MN) in the magnetic field exposed group. However, an in vitro exposure of 1.5 Gy gamma-irradiation caused a significant increase in both DNA damage and MN induction.
  
  
  CONCLUSIONS
  This study found no evidence that an acute, whole-body exposure to a 200 microT, 60 Hz magnetic field for 4 hours could cause DNA damage in human blood.
authors.affiliation: Bioelectromagnetics, Lawson Health Research Institute, Department of Medical Biophysics, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario.
authors.forename: Genevieve C
authors.initials: GC
authors.lastname: Albert
authors.forename: James P
authors.initials: JP
authors.lastname: McNamee
authors.forename: Leonora
authors.initials: L
authors.lastname: Marro
authors.forename: Pascale V
authors.initials: PV
authors.lastname: Bellier
authors.forename: Frank S
authors.initials: FS
authors.lastname: Prato
authors.forename: Alex W
authors.initials: AW
authors.lastname: Thomas
mesh_terms: D000328:Adult; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged