Polymorphism of the sterol regulatory element-binding protein-2 gene and its association with serum lipid levels in the Guangxi Hei Yi Zhuang and Han populations.
BACKGROUND
Information about the association of sterol regulatory element-binding protein-2 (SREBP-2) gene polymorphism at the 1784G>C locus and serum lipid parameters is limited and contradictory. The present study was undertaken to compare the difference in the SREBP-2 gene polymorphism and its association with serum lipid levels between the Guangxi Hei Yi Zhuang and Han populations.


METHODS
Genotyping of the SREBP-2 gene in 768 subjects of Hei Yi Zhuang and 798 participants of Han Chinese aged 15 to 89 was performed by polymerase chain reaction and restriction fragment length polymorphism.


RESULTS
The frequency of G allele was 88.9% in Hei Yi Zhuang and 84.7% in Han (P < 0.001). The frequencies of GG, GC, and CC genotypes were 78.9%, 20.1%, and 1.0% in Hei Yi Zhuang, and 70.4%, 28.6%, and 1.0% in Han (P < 0.001), respectively. The frequencies of G allele and GG genotype in Han, but not in Hei Yi Zhuang, were higher in males than in females, or in high total cholesterol (TC >5.18 mmol/L) subgroup than in normal TC subgroup (P < 0.01 for all). In high TC subgroup, TC, triglycerides, low-density lipoprotein cholesterol, and apolipoprotein B levels in Han were lower in GG genotype than in GC/CC genotype (P < 0.05-0.01).


CONCLUSIONS
There were significant differences in the genotypic and allelic frequencies of the SREBP-2 gene polymorphism at the 1784G>C locus between the Hei Yi Zhuang and Han populations. The individuals carrying the G allele have more favorable lipid profiles than those carrying the C allele in Han but not in Hei Yi Zhuang.
Yaju
Deng
D
Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, Nanning, People's Republic of China.
Ruixing
Yin
Y
Yiyang
Li
L
Yijiang
Zhou
Z
Weixiong
Lin
L
Shangling
Pan
P
Dezhai
Yang
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics*
D008055:Lipids; C497390:SREBF2 protein, human; D051782:Sterol Regulatory Element Binding Protein 2