Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins.
A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation (ATRP) polymerisation of (polyethylene glycol) methacrylate (PEGMA) with surface Si-H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.
Chen
Ling
L
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
Chen
Zeng-Tai
ZT
Wang
Jing
J
Xiao
Shou-Jun
SJ
Lu
Zu-Hong
ZH
Gu
Zhong-Ze
ZZ
Kang
Lin
L
Chen
Jian
J
Wu
Pei-Heng
PH
Tang
Yan-Chun
YC
Liu
Jian-Ning
JN
D005782:Gels / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties
D005782:Gels; D012825:Silicon