Screening of antioxidant phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC.
The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentration-dependent antioxidative capacity was verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results obtained indicate that the in vitro production of B. oleracea var. costata shoots can become important in the obtention of a noticeable dietary source of compounds with health protective potential.
Ferreres
Federico
F
REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal.
Sousa
Carla
C
Pereira
David M
DM
Valentão
Patrícia
P
Taveira
Marcos
M
Martins
Anabela
A
Pereira
José A
JA
Seabra
Rosa M
RM
Andrade
Paula B
PB
D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds; D001937:Brassica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D006997:Hypochlorous Acid; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010851:Picrates; D018520:Plant Shoots / Q000737:chemistry; D013481:Superoxides; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D013481:Superoxides; D006997:Hypochlorous Acid; C004931:1,1-diphenyl-2-picrylhydrazyl