title: Bimatoprost-induced calcium signaling in human T-cells does not involve prostanoid FP or TP receptors.
abstract: OBJECTIVE
  The prostamide bimatoprost and prostanoid FP receptor agonists are highly efficacious drugs for glaucoma treatment. The presence of both prostamide and prostanoid FP receptors in bimatoprost-sensitive preparations has made prostamide receptor classification difficult. This study investigated a novel bimatoprost-sensitive preparation.
  
  
  METHODS
  Human peripheral blood T lymphoblasts (Molt-3) and human osteoblasts (hFOB) were cultured for intracellular calcium signaling studies and quantitative real-time PCR analysis of RNA.
  
  
  RESULTS
  Bimatoprost stimulated concentration-related increases in [Ca(2 +)](i) in a human T-cell line that does not express human FP receptor/variants, according to PCR analysis. The calcium signal induced by bimatoprost was not antagonized by prostanoid FP receptor antagonist/partial agonist AL-8810 or selective TP receptor antagonist SQ 29548. Conversely, bimatoprost did not elevate [Ca(2 +)](i) in human osteoblasts, which were confirmed to contain RNA of human FP receptor/variants.
  
  
  CONCLUSIONS
  Molt-3 cells have been identified as a bimatoprost-sensitive preparation in which the activity of bimatoprost is independent of prostanoid FP receptors.
authors.affiliation: Department of Biological Sciences, Allergan, Inc., Irvine, California 92612, USA. chen_june@allergan.com
authors.forename: June
authors.initials: J
authors.lastname: Chen
authors.forename: Ryan Ta
authors.initials: RT
authors.lastname: Lu
authors.forename: Ronald
authors.initials: R
authors.lastname: Lai
authors.forename: Tim
authors.initials: T
authors.lastname: Dinh
authors.forename: David
authors.initials: D
authors.lastname: Paul
authors.forename: Steven
authors.initials: S
authors.lastname: Venadas
authors.forename: Larry A
authors.initials: LA
authors.lastname: Wheeler
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000959:Antihypertensive Agents / Q000494:pharmacology*; D000069580:Bimatoprost; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D003008:Cloprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017482:Receptors, Thromboxane / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000577:Amides; D000959:Antihypertensive Agents; D011982:Receptors, Prostaglandin; D017482:Receptors, Thromboxane; C034671:prostaglandin F2alpha receptor; D003008:Cloprostenol; D000069580:Bimatoprost; D002118:Calcium