Structures of two monomeric units of teichoic acid prepared from the cell wall of Lactobacillus plantarum NRIC 1068.
The cell wall of Lactobacillus plantarum contains large amounts of cell wall teichoic acid (WTA). WTA was isolated from the cell wall of L. plantarum NRIC 1068 (= ATCC 8014 and 17-5) by extraction with trichloroacetic acid, and two monomeric units (F1 and F2) were prepared from the alkaline hydrolysate of WTA. Componential analysis by HPLC showed that these monomers were composed of ribitol, glucose, and phosphoric acid. Structural analyses of the monomers were performed by NMR spectroscopy with comparison to chemically synthesized monomers. The structures of F1 and F2 were determined to be 3,4-alpha-D-diglucosyl-2-phosphoryl ribitol and 3,4-alpha-D-diglucosyl-1-phosphoryl ribitol respectively. The unique structure of WTA in L. plantarum results from modification of the main chain with multiple glucose residues.
Tomita
Satoru
S
Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Tokyo, Japan. ttagggtelomere@gmail.com
Furihata
Kazuo
K
Nukada
Tomoo
T
Satoh
Eiichi
E
Uchimura
Tai
T
Okada
Sanae
S
D002473:Cell Wall / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D048191:Lactiplantibacillus plantarum / Q000166:cytology*; D009682:Magnetic Resonance Spectroscopy; D010766:Phosphorylation; D013682:Teichoic Acids / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism
D013682:Teichoic Acids