title: Increased plasma dipeptidyl peptidase IV (DPP IV) activity and decreased DPP IV activity of visceral but not subcutaneous adipose tissue in impaired glucose tolerance rats induced by high-fat or high-sucrose diet.
abstract: Several studies have investigated whether dipeptidyl peptidase IV (DPP IV) activity is correlated to the severity of diabetes; however, it remains unclear. To investigate the roles of DPP IV activity in metabolic abnormalities, impaired glucose tolerance rats were produced using a high-fat (HF) or high-sucrose (HS) diet. HF diet-fed rats obviously exhibited impaired glucose tolerance, with increases in subcutaneous and epididymal fat mass, insulin resistance and dyslipidaemia. In rats fed a HS diet rather than a normal diet, lower body weight and fasting blood glucose were observed temporarily in the early period after HS diet feeding; however, impaired glucose tolerance was evoked to some extent with an increase in epididymal fat mass. Both HF and HS diet-fed rats showed significantly higher plasma DPP IV activity than normal diet-fed rats, in the order of HF diet>HS diet>normal diet. HF and HS diets did not significantly affect DPP IV activity and mRNA expression in the kidney. On the other hand, HF, but not HS, diet caused a significant decrease in DPP IV activity in the liver as compared to the control. Of note, both HF and HS diets caused a significant decrease in DPP IV activity in epididymal fat, even though they did not change DPP IV activity in subcutaneous fat. In conclusion, HF or HS diet-induced impaired glucose tolerance with visceral fat accumulation may be interrelated with increased plasma DPP IV activity and decreased DPP IV activity of visceral but not subcutaneous adipose tissue.
authors.affiliation: Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kirino
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kamimoto
authors.forename: Youichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawazoe
authors.forename: Kazuo
authors.initials: K
authors.lastname: Minakuchi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakahori
mesh_terms: D000818:Animals; D001835:Body Weight; D004041:Dietary Fats / Q000008:administration & dosage*; D019422:Dietary Sucrose / Q000008:administration & dosage*; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D004435:Eating; D018149:Glucose Intolerance / Q000201:enzymology*; D005951:Glucose Tolerance Test; D050152:Intra-Abdominal Fat / Q000201:enzymology*; D008297:Male; D009928:Organ Specificity; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D050151:Subcutaneous Fat / Q000201:enzymology*
chemical_list: D004041:Dietary Fats; D019422:Dietary Sucrose; D012313:RNA; D018819:Dipeptidyl Peptidase 4