Production of a reporter transgenic pig for monitoring Cre recombinase activity.
The pig is thought to be the most suitable non-human source of organs for xenotransplantation and is widely used as a model of human disease. Using pigs as disease models requires the design of conditional Cre recombinase-loxP gene modifications, which, in turn, requires a Cre-expressing pig with defined patterns of expression controlled by the use of a tissue-specific promoter. In order to monitor Cre recombinant expression in vivo, it is important to create a reporter strain. We have generated reporter a pig that is based on a single vector that drives the ubiquitous expression of the enhanced green fluorescent protein (EGFP). The EGFP gene is expressed only after Cre-mediated excision of loxP-flanked stop sequences. These reporter transgenic pigs will be of great value for monitoring Cre recombinase activity in vivo.
Li
Li
L
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China. lilitonghappy@hotmail.com
Pang
Daxin
D
Wang
Tiedong
T
Li
Zhanjun
Z
Chen
Limei
L
Zhang
Mingjun
M
Song
Na
N
Nie
Daibang
D
Chen
Zhenwen
Z
Lai
Liangxue
L
Ouyang
Hongsheng
H
D000818:Animals; D030801:Animals, Genetically Modified; D004195:Disease Models, Animal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics*
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases