title: Cloning and characterization of cDNAs encoding ent-copalyl diphosphate synthases in wheat: insight into the evolution of rice phytoalexin biosynthetic genes.
abstract: In vitro assays using recombinant enzymes enabled three cDNAs encoding ent-copalyl diphosphate synthases to be identified in wheat (Triticum aestivum): TaCPS1, TaCPS2, and TaCPS3. The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice.
authors.affiliation: Department of Bioresource Engineering, Yamagata University, Tsuruoka, Yamagata, Japan. toyomasu@tds1.tr.yamagata-u.ac.jp
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Toyomasu
authors.forename: Takuma
authors.initials: T
authors.lastname: Kagahara
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hirose
authors.forename: Masami
authors.initials: M
authors.lastname: Usui
authors.forename: Shiho
authors.initials: S
authors.lastname: Abe
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.forename: Jinichiro
authors.initials: J
authors.lastname: Koga
authors.forename: Wataru
authors.initials: W
authors.lastname: Mitsuhashi
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D019143:Evolution, Molecular; D017343:Genes, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics; D000094162:Phytoalexins
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012717:Sesquiterpenes; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C090963:ent-kaurene synthetase A; D000094162:Phytoalexins