Assessing the marginal error in diagnosis and cure of Schistosoma mansoni in areas of low endemicity using Percoll and PCR techniques.
OBJECTIVE
The Kato-Katz technique misses a proportion of low-intensity schistosomiasis cases. We set out to estimate the rate of false negative results after Kato-Katz.


METHODS
Comparison with the more sensitive Percoll technique and polymerase chain reaction (PCR).


RESULTS
Amongst Kato-Katz negative Schistosoma mansoni specimens in a hypoendemic area, Percoll detected 11% positive cases and PCR detected 23%. After treatment, the cure rate was 97% by Kato-Katz, 90% by Percoll and 71% by PCR. Results of Kato-Katz and Percoll showed moderate agreement, all cases positive by Kato-Katz were positive by Percoll. However, PCR showed some negative results amongst cases considered sure positives.


CONCLUSIONS
The Percoll technique seems to be the technique of choice for diagnosis of S. mansoni in low intensity areas and after treatment.
Allam
Amal Farahat
AF
Medical Research Institute, University of Alexandria, Alexandria, Egypt. amalalam2005@yahoo.com
Kader
Ola
O
Zaki
Adel
A
Shehab
Amel Youssef
AY
Farag
Hoda Fahmy
HF
D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D002499:Centrifugation, Density Gradient / Q000379:methods; D002648:Child; D016054:DNA, Protozoan / Q000032:analysis; D004534:Egypt / Q000453:epidemiology; D005188:False Negative Reactions; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction / Q000379:methods; D011223:Praziquantel / Q000627:therapeutic use; D012550:Schistosoma mansoni / Q000235:genetics / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology; D016896:Treatment Outcome
D000871:Anthelmintics; D016054:DNA, Protozoan; D011223:Praziquantel