[Individual identification and paternity testing of bulls using microsatellite].
The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
Wang
Jing
J
Lanzhou Vocational Technology College, Lanzhou, China. jwang_423@126.com
Liu
Chou-Sheng
CS
Zhang
Li-Ping
LP
Wang
Zhi-Gang
ZG
Yu
Fu-Qing
FQ
Zhang
Gui-Xiang
GX
Song
Xiu-Zhu
XZ
Han
Xu
X
Wei
Yu-Chun
YC
D000818:Animals; D002417:Cattle; D033481:Chromosomes, Mammalian / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D014644:Genetic Variation / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics