title: Determination of lactic acid enantiomers in human urine by high-performance immunoaffinity LC-MS.
abstract: In this study, a monoclonal anti-d-hydroxy acid antibody was used as chiral selector for chromatographic enantiomer separation and quantification of lactic acid contained in human urine samples. The immunoaffinity column was directly coupled to an electrospray ionization mass spectrometer for detection. Separations were performed at room temperature and under isocratic conditions using ammonium bicarbonate buffer (pH 7.8; 10 mM) as mobile phase. No elaborate sample preparation or analyte derivatization was required and individual runs were completed in less than 10 min.
authors.affiliation: Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115-2862, USA.
authors.forename: Elliott J
authors.initials: EJ
authors.lastname: Franco
authors.forename: Heike
authors.initials: H
authors.lastname: Hofstetter
authors.forename: Oliver
authors.initials: O
authors.lastname: Hofstetter
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003404:Creatinine / Q000097:blood; D006801:Humans; D019344:Lactic Acid / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D019344:Lactic Acid; D003404:Creatinine