[Effects of survivin antisense oligodeoxynucleotide on drug resistance in K562 cells].
OBJECTIVE
To study the effect of Survivin antisense oligodeoxgnucleotide (ASODN) on drug resistance and its mechanism in K562 cells.


METHODS
Cells were divided into four groups: ASODN group, SODN group, Lip group and blank group. Survivin ASODN was transfered into K562 cells by liposomal reagent. The expression of Survivin mRNA was detected by RT-PCR. The sensitivity of K562 cells to adriamycin (ADM) and daunorubicin (DAM) was detected by MTT assay, while the intracellular concentration of ADM and DAM was measured by flowcytometry.


RESULTS
Compared to control group Survivin mRNA expression in ASODN group decreased obviously. IC50(s) of ADM and ASODN+ADM were 0.5457 mg/L and 0.1933 mg/L respectively, IC50 (s) of DAM and ASODN+DAM were 0.5408 mg/L and 0.2027 mg/L respectively. Expression of Survivin mRNA decreased by 25.8% after the transfection of ASODN. Fluorescence intensity of ADM and ASODN+ADM in K562 cells were 51.64 and 89.92, Fluorescence intensity of DAM and ASODN+DAM in K562 cells were 63.71 and 88.47.


CONCLUSIONS
Expression of Survivin mRNA in k562 cells was down-regulated by ASODN. Survivin ASODN is able to reverse the drug resistance via inhibition of Survivin expression and inducement of apoptosis.
Yang
Shan
S
Department of Hematology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
Xu
You-hua
YH
Wen
Xian-hao
XH
Chen
Lin
L
Li
Xin
X
D003630:Daunorubicin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D020014:K562 Cells; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D000077022:Survivin; D004317:Doxorubicin; D003630:Daunorubicin