title: Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia boliviana Piper seeds.
abstract: Plant lectins are the most studied group of carbohydrate-binding proteins. Despite the high similarity between the members of the Diocleinae subtribe (Leguminosae) group, they present differing biological activities. Canavalia boliviana lectin (Cbol) was purified using a Sephadex G-50 column and crystallized in the presence of X-Man by hanging-drop vapour diffusion at 293 K. After optimization, crystals suitable for diffraction were obtained under the condition 0.1 M HEPES pH 7.5 and 3.0 M sodium formate. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 126.70, b = 66.64, c = 64.99 A, alpha = 90.0, beta = 120.8, gamma = 90.0 degrees . Assuming the presence of a dimer in the asymmetric unit, the solvent content was estimated to be about 46%. A complete data set was collected at 1.5 A resolution.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Tales Rocha
authors.initials: TR
authors.lastname: Moura
authors.forename: Gustavo Arruda
authors.initials: GA
authors.lastname: Bezerra
authors.forename: Maria Julia Barbosa
authors.initials: MJ
authors.lastname: Bezerra
authors.forename: Cícero Silvano
authors.initials: CS
authors.lastname: Teixera
authors.forename: Eduardo Henrique Salviano
authors.initials: EH
authors.lastname: Bezerra
authors.forename: Raquel Guimarães
authors.initials: RG
authors.lastname: Benevides
authors.forename: Bruno Anderson Matias
authors.initials: BA
authors.lastname: da Rocha
authors.forename: Luiz Augusto Gomes
authors.initials: LA
authors.lastname: de Souza
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D032449:Canavalia / Q000737:chemistry*; D002846:Chromatography, Affinity; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D037121:Plant Lectins / Q000032:analysis / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D037121:Plant Lectins; C039268:lectin, Canavalia