Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells.
Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.
Wang
Jun-Jie
JJ
Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, PR China.
Fu
Xiu-Qing
XQ
Guo
Yu-Guang
YG
Yuan
Lin
L
Gao
Qian-Qian
QQ
Yu
Hua-Li
HL
Shi
Heng-Liang
HL
Wang
Xing-Zhi
XZ
Xiong
Wen-Cheng
WC
Zhu
Xiao-Juan
XJ
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D051379:Mice; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
C409064:Myo10 protein, mouse; D011993:Recombinant Fusion Proteins; D007987:Gonadotropin-Releasing Hormone; D009218:Myosins