Gene targeting in mouse embryonic stem cells.
The scientific value of a mouse model with a targeted mutation depends greatly upon how carefully the mutation has been engineered. Until recently, our ability to alter the mouse genome has been limited by both the lack of technologies to conditionally target a locus and by conventional cloning. The "cre/loxP" and "recombineering" technologies have overcome some of these limitations and have greatly enhanced our ability to manipulate the mouse genome in a sophisticated way. However, there are still some practical aspects that need to be considered to successfully target a specific genetic locus. Here, we describe the process to engineer a targeted mutation to generate a mouse model. We include a tutorial using the publicly available informatic tools that can be downloaded for processing the genetic information needed to generate a targeting vector.
Tessarollo
Lino
L
Mouse Cancer Genetics Program, NCI-Frederick, Frederick, MD, USA.
Palko
Mary Ellen
ME
Akagi
Keiko
K
Coppola
Vincenzo
V
D000818:Animals; D001483:Base Sequence; D053595:Embryonic Stem Cells / Q000502:physiology*; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid