Zwitterionic molecularly imprinted polymer-based solid-phase micro-extraction coupled with molecularly imprinted polymer sensor for ultra-trace sensing of L-histidine.
The proposed L-histidine sensing system composed of a molecularly imprinted solid-phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non-specific sorption and false-positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid-phase microextraction material. The hyphenated approach was able to detect L-histidine quantitatively with a limit of detection as low as 0.0435 ng/mL (RSD = 0.2%, S/N = 3).
Prasad
Bhim Bali
BB
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India. prof.bbpd@yahoo.com
Tiwari
Khushaboo
K
Singh
Meenakshi
M
Sharma
Piyush S
PS
Patel
Amit K
AK
Srivastava
Shrinkhala
S
D006639:Histidine / Q000032:analysis*; D007477:Ions / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods*
D007477:Ions; D011108:Polymers; D006639:Histidine