Velocity gap theory developed for magnifying resolutions without changing separation mechanisms or separation lengths.
Separation techniques, such as chromatography and electrophoresis, form the basis in many fields and are continually developed for better separation efficiency. The efforts normally involve a new mechanism together with sufficient separation length. We develop a velocity gap theory to make things simple. The theory is based on the discovery that the velocity gap (VG) effect could enlarge the distance between two moving objects. Mathematical deduction certified that the resolution may be magnified infinitely without changing the separation mechanism or the separation length. DNA separation confirmed its practical feasibility by achieving 2-5 times higher resolution on a microchip. Our results indicate that VG effect could enlarge the distance between two moving objects and may potentially be utilized to ameliorate separation efficiency.
Zhang
Yong
Y
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan. yl7173@gmail.com
Wang
Jun
J
Okamoto
Yukihiro
Y
Tokeshi
Manabu
M
Kaji
Noritada
N
Baba
Yoshinobu
Y
D001888:Boric Acids / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D004492:Edetic Acid / Q000737:chemistry; D005240:Feasibility Studies; D009038:Motion; D014325:Tromethamine / Q000737:chemistry
D001888:Boric Acids; C069591:Tris-borate-EDTA buffer; D014325:Tromethamine; D004247:DNA; D004492:Edetic Acid