title: Parenteral nutrition maintains pulmonary IgA antibody transport capacity, but not active transport, following injury.
abstract: BACKGROUND
  Parenteral nutrition (PN) increases post-trauma pneumonia versus enteral feeding. PN impairs murine immunoglobulin A (IgA) airway defenses and abrogates a normal IgA increase following injury. This work investigates the effect of type/route of nutrition on lung IgA and its transport protein, polymeric immunoglobulin receptor (pIgR), after injury.
  
  
  METHODS
  Catheterized mice were randomized to Chow or PN for 5 days and sacrificed without injury (Chow: n = 12; PN n = 11), or 8 hours after laparotomy + neck incisions (Chow-injury: n = 11, PN-injury: n = 13). Bronchoalveolar lavage (BAL) and lung IgA levels were analyzed by enzyme-linked immunosorbent assay (ELISA) and lung pIgR by Western blot.
  
  
  RESULTS
  BAL IgA levels increased in Chow-injury versus PN-injury (P <.01) with no differences in pIgR. PN-injury tissue IgA levels decreased versus Chow (P <.01), Chow-injury (P <.01), and PN (P <.05).
  
  
  CONCLUSIONS
  PN impairs the airway IgA response to injury but not due to impaired IgA transport capacity/pIgR level.
authors.affiliation: Department of Surgery, University of Wisconsin-Madison, College of Medicine, Public Health, Madison, WI, USA.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Sano
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Hermsen
authors.forename: Woodae
authors.initials: W
authors.lastname: Kang
authors.forename: F Enrique
authors.initials: FE
authors.lastname: Gomez
authors.forename: Jinggang
authors.initials: J
authors.lastname: Lan
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Maeshima
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Kudsk
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007070:Immunoglobulin A / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010288:Parenteral Nutrition / Q000379:methods*; D011379:Prognosis; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism*; D014947:Wounds and Injuries / Q000276:immunology / Q000378:metabolism / Q000628:therapy*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A