Heterotypic interaction of fibroblasts and endothelial cells on restricted area.
The polysaccharide hyaluronic acid (Hyal) was photoimmobilized on glass surfaces to obtain a pattern with squares and rectangles of different dimensions and chemistry. The microstructured surfaces were characterized by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Attenuated Total Reflection Infrared Spectroscopy (ATR FT-IR), and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). Surface analysis revealed the presence of a pattern consisting of alternating glass and Hyal microstructures whose dimensions decreased from the center to the edge of the sample. The behavior of Human Coronary Artery Endothelial Cells (HCAEC) and human tumoral dermal fibroblasts (C54) was studied on these micropatterned surfaces. Neither HCAEC nor C54 adhered to the immobilized Hyal but both adapted their shape to the different sizes of the glass squares and rectangles. The number of adherent HCAEC and C54 depended on the dimensions of both the glass domains and the nuclei of the cells. Co-cultured C54 on HCAEC patterned surfaces showed a heterotypic cell-cell interaction in the same chemical and topographic domain for the first time. In comparison to other techniques for patterning two different cell types, our approach was non cytotoxic and allowed arbitrary geometric patterns to form on different biocompatible substrata.
Lamponi
S
S
Department of Chemical and Biosystems Science and Technologies and CRISMA, University of Siena, Via A. Moro, 2, Siena 53100, Italy.
Di Canio
C
C
Forbicioni
M
M
Barbucci
R
R
D002448:Cell Adhesion; D002452:Cell Count; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D048430:Cell Shape; D002478:Cells, Cultured; D018920:Coculture Techniques; D003593:Cytoplasm / Q000648:ultrastructure; D042783:Endothelial Cells / Q000502:physiology* / Q000648:ultrastructure; D005347:Fibroblasts / Q000502:physiology* / Q000648:ultrastructure; D005898:Glass; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D013058:Mass Spectrometry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D018715:Microscopy, Video; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared
D006820:Hyaluronic Acid