title: Highly sensitive and reproducible cyclodextrin-modified gold electrodes for probing trace lead in blood.
abstract: A highly sensitive and reproducible lead sensor based on a cyclodextrin-modified gold electrode was created. A self-assembled monolayer (SAM) of thiolated beta-cyclodextrin (6-(2-mercapto-ethylamino)-6-deoxy-beta-cyclodextrin (MEA-beta-CD)) was prepared and modified on a gold electrode (MCGE) for specific Pb(2+)-sensing. Thus the mercury-free sensors for Pb(2+) assay based on MCGE were established. A linear calibration response for Pb(2+) was found in the range of 1.7 x 10(-8)M to 9.3 x 10(-7)M. The detection limit was 7.1 x 10(-9)M (with S/N>3), which was 10 times lower than other reported methods of detection Pb(2+) with CD. The measurement results via this method for real blood samples were well agree with those obtained by ICP-AES, and thus presented a novel strategy in design of specific lead sensors with high sensitivity and stability for analysis of trace Pb(2+) in real blood samples.
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University, Handan Rd. 220#, Shanghai 200433, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guiying
authors.initials: G
authors.lastname: Jin
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D003505:Cyclodextrins; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D006046:Gold; D006801:Humans; D007854:Lead / Q000097:blood*
chemical_list: D003505:Cyclodextrins; D007854:Lead; D006046:Gold