The stationary-phase sigma factor sigma(S) is responsible for the resistance of Escherichia coli stationary-phase cells to mazEF-mediated cell death.
Escherichia coli mazEF is a toxin-antitoxin gene module that mediates cell death during exponential-phase cellular growth through either reactive oxygen species (ROS)-dependent or ROS-independent pathways. Here, we found that the stationary-phase sigma factor sigma(S) was responsible for the resistance to mazEF-mediated cell death during stationary growth phase. Deletion of rpoS, the gene encoding sigma(S) from the bacterial chromosome, permitted mazEF-mediated cell death during stationary growth phase.
Kolodkin-Gal
Ilana
I
Department of Molecular Biology, the Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.
Engelberg-Kulka
Hanna
H
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D015169:Colony Count, Microbial; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004722:Endoribonucleases / Q000096:biosynthesis*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D050296:Microbial Viability; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C422954:MazE protein, E coli; C422955:MazF protein, E coli; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D004722:Endoribonucleases