title: Rapid detection of Salmonella in foods using real-time PCR.
abstract: Conventional methods for detection of Salmonella serovars in foods are generally time-consuming and labor intensive. A real-time PCR method has been developed with custom designed primers and a TaqMan probe to detect the presence of a 262-bp fragment of the Salmonella-specific invA gene. The method has been tested with a total of 384 field-isolated Salmonella serovars and non-Salmonella stock strains, as well as 420 U.S. Food and Drug Administration food samples, comprising a variety of food matrices. The method was highly specific in detecting Salmonella in spiked chili powder and shrimp samples, with a sensitivity of 0.04 CFU/g. In addition, the method is faster, more accurate, and less costly than the traditional U.S. Food and Drug Administration's Bacteriological Analytical Manual cell-culturing and the AOAC International-approved VIDAS methods to detect Salmonella in foods.
authors.affiliation: U.S. Food and Drug Administration, Pacific Regional Laboratory Southwest, Irvine, California 92612, USA. chorng-ming.cheng@fda.hhs.gov
authors.forename: Chorng-Ming
authors.initials: CM
authors.lastname: Cheng
authors.forename: Wen
authors.initials: W
authors.lastname: Lin
authors.forename: Khanh Thien
authors.initials: KT
authors.lastname: Van
authors.forename: Lieuchi
authors.initials: L
authors.lastname: Phan
authors.forename: Nelly N
authors.initials: NN
authors.lastname: Tran
authors.forename: Doris
authors.initials: D
authors.lastname: Farmer
mesh_terms: D001426:Bacterial Proteins; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005784:Gene Amplification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019779:Salmonella enterica / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C075518:invA protein, Bacteria