[Study the effect of antisense MMP-2 by transfection of ad-aMMP-2 on vascular endothelial cells from human proferating hemangiomas in vitro].
OBJECTIVE
To investigate the effect of antisense MMP-2 by transfecting the Ad-aMMP-2 on the vascular endothelial cells (VEC) derived from human proferating hemangiomas in vitro.


METHODS
Three groups. Group M199, Group Ad-GFP and Group Ad-aMMP-2 were tested in this study with the 100 multiplicity of infection (MOI). Reverse transcription PCR (RT-PCR), Western Blotting and Gelatin Zymography analyses were applied to evaluate the level of endogenous MMP-2 expression.


RESULTS
The decreased expression level of endogenous MMP-2 mRNA and protein excretion of MMP-2 and Gelatin Zymography analyses of liveness of Group Ad-aMMP-2 were observed by comparison with Group M199 and Group Ad-GFP (P<0.05).


CONCLUSIONS
Ad-aMMP-2 could inhibit the secretion of MMP-2 from VEC in vitro.
Zeng
Fan-wei
FW
Department of Reconstructive and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Cen
Yin-ga
YG
Liu
Xiao-xue
XX
Xu
Xue-wen
XW
Chen
Jun-jie
JJ
Li
Zheng-yong
ZY
Wu
Dong-mei
DM
D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics / Q000494:pharmacology; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006392:Hemangioma, Cavernous / Q000473:pathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000473:pathology; D014162:Transfection
D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2