title: Association of cytokine gene polymorphisms with chronic obstructive pulmonary disease in Macedonians.
abstract: The aim of this study was to examine the association of 22 cytokine gene polymorphism in Macedonians with chronic obstructive pulmonary disease (COPD). The sample of the population comprised of 301 normal respondents and 62 patients with COPD. Cytokine genotyping was performed by polymerase chain reaction with sequence-specific priming (PCR-SSP). Positive (susceptible) association was found between patient with COPD and IL-1alpha -889/C allele; where as negative (protective) association among was found for the following alleles IL-1beta +3962/C; IL-12B -1188/A; IFNgamma +874/T; IL-2 -330/G; IL-4 -1098/G and IL-4-33/C. We found positive (susceptible) association between patients with COPD and following genotypes: IL4 -33/T:T; IFNgamma +874/A:A; IL-4 -1098/T:T ; IL-1alpha -889/C:C; IL-1beta +3962/C:T; IL-12B -1188/C:C; IL-4Ralpha +1902/G:G; IL-10 -1082/G:G; IL-2 -330/T:T; IL-4 -590/C:C; and IL-1alpha -889/C:T. Negative (protective) association between patients with COPD and following genotypes was found: IFNgamma +874/A:T; IL-4 -33/C:T; IL-4 -1098/G:T; IL-2 -330/G:T; IL-1beta +3962/C:T; IL-4 -590/C:T; IL-10 -1082/A:G; and IL-4 -33/C:C. Positive (susceptible) association between patients with COPD and following haplotypes was found: IL-4/TCT; IL-10/ATC; and IL-2/TG, and negative (protective) association was found between the patients with COPD and haplotypes for: IL-4/TTC; and IL-4/GCC. It could be concluded that several cytokine polymorphisms are positively (susceptible), or negatively (protective) associated with COPD in Macedonians.
authors.affiliation: Institute of Immunobiology and Human Genetics, University School of Medicine Ss. Kiril and Metodij, Skopje, Republic of Macedonia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Trajkov
authors.forename: Jagoda
authors.initials: J
authors.lastname: Mirkovska-Stojkovikj
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petlichkovski
authors.forename: Ana
authors.initials: A
authors.lastname: Strezova
authors.forename: Olivija
authors.initials: O
authors.lastname: Efinska-Mladenovska
authors.forename: Emilija
authors.initials: E
authors.lastname: Sandevska
authors.forename: Olgica
authors.initials: O
authors.lastname: Sibinovska
authors.forename: Slavica
authors.initials: S
authors.lastname: Hristomanova
authors.forename: Eli
authors.initials: E
authors.lastname: Djulejic
authors.forename: Jordan
authors.initials: J
authors.lastname: Petrov
authors.forename: Jean
authors.initials: J
authors.lastname: Gogusev
authors.forename: Mirko
authors.initials: M
authors.lastname: Spiroski
mesh_terms: D000328:Adult; D002908:Chronic Disease; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D053662:Interleukin-4 Receptor alpha Subunit / Q000235:genetics; D007378:Interleukins / Q000235:genetics; D008173:Lung Diseases, Obstructive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018843:Republic of North Macedonia; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D055815:Young Adult
chemical_list: D016207:Cytokines; D053662:Interleukin-4 Receptor alpha Subunit; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma