title: [Detection of hearing threshold and polymorphic molecular marker analysis of guinea pigs of mimetic aging].
abstract: OBJECTIVE
  To explore the establishment of the mimetic aging effect in guinea pigs induced by D-galactose, and to detect the biological indicatrix associated with hearing loss and provide a new tool for molecular pathogenesis of hearing loss.
  
  
  METHODS
  Total of 51 guinea pigs were randomly divided into three groups: group A (model aging group, n = 25), which were injected with D-galactose (200 mgxkg(-1)xd(-1)) by intra peritoneum for 6 weeks, group B (model control group, n = 18), which were given the same amount of saline only, and group C (vacant group, n = 15) were not treated. Then, The guinea pigs in group A and B were exposed in noise for 8 days, 8 hours once a day. Auditory brainstem response (ABR) was used to test the hearing threshold of guinea pigs thrice, first before the drug administered, then after 6 weeks the drug used, third after noise exposure. And colorimetry was used to analyze the activity of superoxide dismutase (SOD) and malon dialdehyde (MDA) in brain and liver tissue. The DNA of inner ear tissue was harvested and amplified fragment length polymorphism (AFLP) was used to detect the differential polymorphic markers.
  
  
  RESULTS
  After injection, there was no significant difference in elevation of ABR threshold between the group A and group B (t = 1.14, P > 0.05). However, exposure of noise later, elevation in ABR threshold of (22.97 +/- 10.56) dB peSPL was observed in group A, and (14.16 +/- 7.36) dB peSPL in group B. The was significant difference in variation of hearing threshold between group A and group B (t = 2.78, P < 0.05). The activity of SOD in brain and liver tissue in group A was lower than that in group B. the level of MDA was opposite between group A and group B. The difference between group A and group B was significant (P < 0.01). A differential polymorphic marker was observed by AFLP.
  
  
  CONCLUSIONS
  The mimetic aging effect of the guinea pigs can be induced by D-galactose, and this model can not directly induce the hearing loss. The differential polymorphic marker possibly act as a predisposing factor which can greatly enhance the sensitivity of the ear to the noise.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Second Affiliated Hospital of Guangzhou Medical College, China.
authors.forename: Min
authors.initials: M
authors.lastname: Mao
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Zhang
authors.forename: Ai-lin
authors.initials: AL
authors.lastname: Tao
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Yan
authors.forename: Shun-lin
authors.initials: SL
authors.lastname: Ouyang
authors.forename: Xu-ping
authors.initials: XP
authors.lastname: Yuan
authors.forename: Li-zi
authors.initials: LZ
authors.lastname: Qiu
authors.forename: Yang
authors.initials: Y
authors.lastname: Xie
authors.forename: Ze-hong
authors.initials: ZH
authors.lastname: Zou
authors.forename: Jie-zhen
authors.initials: JZ
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D000375:Aging; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001309:Auditory Threshold; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D005690:Galactose / Q000494:pharmacology; D006168:Guinea Pigs; D008297:Male; D011304:Presbycusis / Q000139:chemically induced / Q000503:physiopathology*
chemical_list: D005690:Galactose