[Influence of JDTL injection on inflammatory factors in rat experimental cerebral ischemia].
OBJECTIVE
To stydy the protective mechanism of Jiedu Tongluo (JDTL) injection on experimental cerebral ischemia.


METHODS
Radioimmunoassay (RIA) was utilized to identify the content of TNF-alpha and IL1-beta in brain tissue of rats suffered from 6 h and 24 h ischemia with MCAO, Enzyme-linked immunoadsordent assay (ELISA) was used to observe the level of MCP-1 and ICMA-1.


RESULTS
After 6 h and 24 h of ischemia, the increased contents of TNF-alpha, IL1-beta, MCP-1 as well as ICMA-1 was observed. JDTL injection dramatically inhibited the increase of TNF-alpha, IL1-beta after 6 h and 24 h of ischemia.


CONCLUSIONS
JDTL injection had inhibiting effect on TNF-alpha, IL1-beta, MCP-1 as well as ICMA-1 caused by cerebral ischemia, the result suggested that JDIL injection can protect cerebral tissue from ischemic injury by inhibiting inflammatory factors.
Yang
Hong
H
Medical Experiment Center of China Academy of Chinese Medical Sciences, Beijing 100700, China. leslie18029@163.com
Zhao
Yi-jun
YJ
Wang
Yong-yan
YY
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000276:immunology; D002545:Brain Ischemia / Q000188:drug therapy / Q000276:immunology* / Q000517:prevention & control*; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators