title: Noninvasive measurement of oxygen saturation of the microvascular blood in Barrett's dysplasia by use of optical spectroscopy.
abstract: BACKGROUND
  Current investigations into endoscopic screening for the early detection of Barrett's esophageal adenocarcinoma have focused on visualization of the microvascular morphology by using narrow-band imaging (NBI). Adjustment of the center wavelength, particularly of the NBI blue imaging filter, may lead to improved image contrast, depending on the oxygen saturation of the microvascular blood of dysplastic and early cancerous Barrett's mucosa.
  
  
  OBJECTIVE
  To perform in vivo, noninvasive measurements of the oxygen saturation of the microvascular blood for different pathologic grades of Barrett's mucosa by using differential path-length spectroscopy (DPS).
  
  
  METHODS
  DPS measurements were made on normal (n = 7), low-grade dysplastic (n = 10), high-grade dysplastic (n = 7), and cancerous (n = 4) Barrett's mucosa by using a fiber-optic probe, and were correlated to the histologic outcome of biopsy specimens taken from the same location.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Fifteen patients with Barrett's esophagus who were undergoing gastroscopy.
  
  
  METHODS
  Biopsy specimens were taken from suspicious areas in the esophagus.
  
  
  METHODS
  The oxygen saturation of the microvascular blood of different pathologic grades of Barrett's mucosa was assessed.
  
  
  RESULTS
  The oxygen saturation of the microvascular blood remains high (approximately 90%) throughout the metaplasia-dysplasia-adenocarcinoma sequence.
  
  
  CONCLUSIONS
  The small number of patients.
  
  
  CONCLUSIONS
  The current NBI blue imaging filter, centered on the peak absorption of oxyhemoglobin (415 nm), is well chosen, and little improvement in image contrast is to be expected from changes in this center wavelength.
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Radiation Oncology, Erasmus Medical Centre, Rotterdam, The Netherlands. a.amelink@erasmusmc.nl
authors.forename: Arjen
authors.initials: A
authors.lastname: Amelink
authors.forename: Jelle
authors.initials: J
authors.lastname: Haringsma
authors.forename: Henricus J C M
authors.initials: HJ
authors.lastname: Sterenborg
mesh_terms: D001471:Barrett Esophagus / Q000097:blood* / Q000473:pathology / Q000503:physiopathology; D001706:Biopsy; D003937:Diagnosis, Differential; D005260:Female; D005336:Fiber Optic Technology / Q000379:methods; D005773:Gastroscopy / Q000379:methods; D006801:Humans; D007413:Intestinal Mucosa / Q000098:blood supply*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption / Q000502:physiology*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D010100:Oxygen