Stable and nondisruptive in vitro/in vivo labeling of mesenchymal stem cells by internalizing quantum dots.
Progress in stem cell research has prioritized the refinement of cell-labeling techniques for in vitro and in vivo basic and therapeutic studies. Although quantum dots, because of their optical properties, are emerging as favorable nanoparticles for bioimaging, substantial refinements or modifications that would improve their biocompatibility are still required. We report here that internalizing quantum dots (i-QDs) generated by their conjugation with an internalizing antibody against a heat shock protein-70 family stress chaperone, mortalin, offered an efficient, genetically noninvasive, nontoxic, and functionally inert way to label mesenchymal stem cells (MSCs). The i-QD-labeled MSCs underwent normal adipocyte, osteocyte, and chondrocyte differentiation in vitro and in vivo, suggesting the potential application of i-QDs in in vivo diagnostics, regenerative and therapeutic medicine.
Ohyabu
Yoshimi
Y
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Kaul
Zeenia
Z
Yoshioka
Tomokazu
T
Inoue
Kazuki
K
Sakai
Shinsuke
S
Mishima
Hajime
H
Uemura
Toshimasa
T
Kaul
Sunil C
SC
Wadhwa
Renu
R
D050156:Adipogenesis; D000818:Animals; D018929:Cell Culture Techniques; D020219:Chondrogenesis; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology; D000882:Haplorhini; D006801:Humans; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D045663:Quantum Dots; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
D018840:HSP70 Heat-Shock Proteins; C080352:mortalin