title: A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer.
abstract: OBJECTIVE
  With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer.
  
  
  METHODS
  GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach.
  
  
  RESULTS
  Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90.
  
  
  CONCLUSIONS
  These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
authors.affiliation: Lab of Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, 210029 Nanjing, Jiangsu, People's Republic of China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Chen
authors.forename: Muzi
authors.initials: M
authors.lastname: Zhao
authors.forename: Saiqun
authors.initials: S
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Cao
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Xu
authors.forename: Guoqing
authors.initials: G
authors.lastname: Tong
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D015854:Up-Regulation / Q000235:genetics
chemical_list: D003598:Cytoskeletal Proteins; C478878:DNAAF4 protein, human; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger