title: Wound healing in colonic anastomosis in hypothyroidism.
abstract: Colonic anastomosis healing in hypothyroidism was evaluated. Twenty Wistar rats were separated into two groups: the study group (n = 10), which underwent total thyroidectomy to induce hypothyroidism, and the control group (n = 10). After 10 weeks, hypothyroid status was confirmed by blood tests (p < 0.0001). Three days later, a colonic anastomosis was performed, and the animals were sacrificed 1 week later. The colon containing the anastomosis was resected for bursting strength tests and histological studies. The slides were stained with hematoxylin-eosin and with Sirius red to quantify and classify the collagen. Reduced values were found for the bursting strength test (p = 0.0006) and for collagen density in the experimental group. There was a prevalence of immature type III collagen (p < 0.0001) and a decrease in mature type I collagen (p < 0.0001) in the hypothyroid group.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, University Hospital, Curitiba, Brasil. ana.nassif@sulbbs.com
authors.forename: A C
authors.initials: AC
authors.lastname: Thá Nassif
authors.forename: F
authors.initials: F
authors.lastname: Hintz Greca
authors.forename: H
authors.initials: H
authors.lastname: Graf
authors.forename: J C
authors.initials: JC
authors.lastname: Domingues Repka
authors.forename: L S
authors.initials: LS
authors.lastname: Nassif
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000473:pathology / Q000601:surgery*; D007037:Hypothyroidism / Q000503:physiopathology*; D008297:Male; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing