title: Protective action of aqueous black tea (Camellia sinensis) extract (BTE) against ovariectomy-induced oxidative stress of mononuclear cells and its associated progression of bone loss.
abstract: The protective action of aqueous black tea extract (BTE) against ovariectomy-induced oxidative stress of mononuclear cells and its associated progression of bone loss was demonstrated in this study. Eighteen female adult 6-month-old Wistar albino rats were divided into three groups: sham-control (A), bilaterally ovariectomized (B) and bilaterally ovariectomized + BTE supplemented (C). Studies included the measurement of oxidative (nitric oxide, lipid peroxidation) and antioxidative (superoxide dismutase, catalase) markers, inflammatory cytokines (IL-6, TNF-alpha), osteoclast differentiation factor (RANKL) and bone resorption markers (tartrate-resistant acid phosphatase and hydroxyproline). Also quantitative histomorphometry and histological studies were undertaken. The bone breaking force was measured. The results indicate that BTE was effective in preserving and restoring skeletal health by reducing the number of active osteoclasts. Such changes with BTE supplementation were steadily linked with the reduced oxidative stress of mononuclear cells, serum levels of bone resorbing cytokines, osteoclast differentiation factor and resorption markers. The results of the bone breaking force, histological and histomorphometric analyses further supported the hypothesis. This study suggests that BTE has both protective and restorative actions against ovariectomy-induced mononuclear cell oxidative stress and associated bone loss.
authors.affiliation: Department of Physiology, Presidency College, Kolkata 700 073, India.
authors.forename: Asankur Sekhar
authors.initials: AS
authors.lastname: Das
authors.forename: Maitrayee
authors.initials: M
authors.lastname: Mukherjee
authors.forename: Dolan
authors.initials: D
authors.lastname: Das
authors.forename: Chandan
authors.initials: C
authors.lastname: Mitra
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000517:prevention & control*; D001842:Bone and Bones / Q000473:pathology; D028241:Camellia sinensis / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D005260:Female; D006909:Hydroxyproline / Q000652:urine; D007527:Isoenzymes / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation; D009569:Nitric Oxide / Q000378:metabolism; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase; D013662:Tea / Q000737:chemistry
chemical_list: D016207:Cytokines; D007527:Isoenzymes; D010936:Plant Extracts; D013662:Tea; D009569:Nitric Oxide; D002374:Catalase; D013482:Superoxide Dismutase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D006909:Hydroxyproline