title: [Asbestos fibre lung burden and exposure indices in asbestos-cement workers].
abstract: BACKGROUND
  In many previous studies, the asbestos fibres retained in the lung were regarded as a good index of cumulative occupational asbestos exposure. Twelve workers suffering from asbestos-related diseases and had been employed in an asbestos-cement factory operating from 1961 to 1994 underwent post mortem investigations in the course of a criminal law suit.
  
  
  OBJECTIVE
  Samples of lung tissues were collected for electron microscopy analysis to measure the asbestos fibre burden of the lungs in workers with high exposure, and assess the possible correlation between asbestos fibre lung burden and the estimated levels of cumulative exposure.
  
  
  METHODS
  Samples of lung parenchyma obtained from a consecutive series of 12 post-mortem examinations that were performed between 1994 and 2007and included 5 cases of malignant pleural mesothelioma, 4 lung cancers, 1 case of asbestosis and2 ofpleuralplagues, were collected, stored and analysed by SEM electron microscopy, according to the methods suggested by the current scientific literature. For each worker, all males, a detailed occupational history was reconstructed by means ofpersonal interviews; both the measurements of airborne asbestos fibresperformed by the factory in the 1970's and the duration of each single job in the plant were taken into account to estimate an individual cumulative exposure index.
  
  
  RESULTS
  A wide variation of total asbestos fibre concentrations in the lung (1,320-118 million) was observed; in all 12 workers, the lung amphibole fibre burden exceeded 1,000,000 fibres per g/dry tissue, The highest values were detected in the mesothelioma cases, in which the mean fibre concentrations differed statistically (t=2.29, p=0.045) from the mean calculated for the other asbestos-related diseases; in 9 subjects only amphibole fibres were detected. There was a good correlation between total asbestos fibre concentration and cumulative exposure index (r=0.91, p<0.0001).
  
  
  CONCLUSIONS
  This study, which was numerically the biggest ever performed in Italy for this category of workers, confirms a wide range of total asbestos fibre burden in heavily occupationally exposed workers and showed that of the asbestos-related diseases, the highest lung concentrations of asbestos fibres were reached in cases of mesothelioma. It was also observed that almost the entire lung burden consists of only amphibole fibres, all exceeding 1 million per gramme of dry tissue. This study tested a synthetic cumulative occupational exposure index, which appears to be well correlated to the level of exposure established by biological analysis.
authors.affiliation: Servizio Prevenzione e Sicurezza Ambienti di Lavoro, ASL Brescia, UO Medicina del Lavoro. pietro.barbieri@aslbrescia.it
authors.forename: P G
authors.initials: PG
authors.lastname: Barbieri
authors.forename: Anna
authors.initials: A
authors.lastname: Somigliana
authors.forename: Sandra
authors.initials: S
authors.lastname: Lombardi
authors.forename: R
authors.initials: R
authors.lastname: Girelli
authors.forename: A
authors.initials: A
authors.lastname: Benvenuti
mesh_terms: D000368:Aged; D017639:Asbestos, Amosite / Q000009:adverse effects / Q000032:analysis*; D017638:Asbestos, Crocidolite / Q000009:adverse effects / Q000032:analysis*; D001195:Asbestosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D003252:Construction Materials / Q000009:adverse effects*; D004577:Electron Probe Microanalysis; D005355:Fibrosis; D006801:Humans; D007558:Italy; D008168:Lung / Q000737:chemistry*; D008175:Lung Neoplasms / Q000737:chemistry / Q000209:etiology / Q000648:ultrastructure; D008297:Male; D008654:Mesothelioma / Q000737:chemistry / Q000209:etiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D019016:Mineral Fibers / Q000009:adverse effects; D009784:Occupational Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D016273:Occupational Exposure / Q000145:classification*; D009790:Occupations; D010994:Pleura / Q000737:chemistry / Q000648:ultrastructure; D010997:Pleural Neoplasms / Q000737:chemistry / Q000209:etiology / Q000648:ultrastructure
chemical_list: D019016:Mineral Fibers; D017638:Asbestos, Crocidolite; D017639:Asbestos, Amosite