Molecular genetic characterization of the merozoite surface protein 1 Gene of Plasmodium vivax from reemerging Korean isolates.
Plasmodium vivax merozoite surface protein 1 (PvMSP-1) has been considered a major candidate for the development of an antimalaria vaccine, but the molecule exhibits antigenic diversity among isolates. The extent of genetic polymorphism in the region between interspecies conserved blocks 4 and 5 (ICB4 and ICB5) of the PvMSP-1 gene was analyzed for 30 Korean isolates. Two genotypes, SK-A and SK-B, were identified on the basis of amino acid substitution. Almost all the amino acid sequences of the Korean isolates were nearly identical to those of the Solomon Island isolate Solo-83 (97.8 to 99.9% similarity) and Philippine isolates Ph-79, Ph-52-2, and Ph-49 (97.3 to 99.8% similarity). Also, we report two sequences in the isolates that were characterized on the basis of restriction fragment length polymorphism (RFLP). The RFLP profiles following digestion with the DraI restriction enzyme produced two distinguishable patterns. This study might be the first report of the region between ICB4 and ICB5 of the MSP-1 gene of P. vivax in South Korea.
Kim
So-Hee
SH
Department of Malariology, Paik Institute for Clinical Research, Inje University College of Medicine, Jin-gu, Busan, South Korea.
Hwang
Seung-Young
SY
Shin
Jeong Hwan
JH
Moon
Chi-Sook
CS
Kim
Dong-Wook
DW
Kho
Weon-Gyu
WG
D000328:Adult; D019943:Amino Acid Substitution; D000818:Animals; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D007723:Korea / Q000453:epidemiology; D016780:Malaria, Vivax / Q000453:epidemiology* / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D055815:Young Adult
D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1