A cell strain cloned from Spodoptera exigua cell line (IOZCAS-Spex-II) highly susceptible to S. exigua nucleopolyhedrovirus infection.
A cell strain (IOZCAS-Spex-II-A) cloned from IOZCAS-Spex-II, a cell line established from the fat body of Spodoptera exigua (Lepidoptera: Noctuidae) larva, was characterized, and its capability to produce S. exigua nucleopolyhedrovirus was high with infection rate exceeding 90% compared with its parental cell line IOZCAS-Spex-II that scored only 50%. Growth curve of budded virus (BV) in the strain was analyzed and the titer of BV reached the highest of 3.7 x 10(4) pfu/mL by 96 h after inoculation. Concentration of occlusion bodies (OBs) produced by the cloned cell strain (IOZCAS-Spex-II-A) was 7.1 x 10(7) OBs/mL, while the parental cell line produced 2.4 x 10(7) OBs/mL. The average yield of the virus was 176 OBs/cell of IOZCAS-Spex-II-A compared with 211 OBs/cell that of the parental cell line. Significant differences were observed in virus production, growth characters, cell shape, between the parental cell line, and its clone. The cell lines (IOZCAS-Spex-II and IOZCAS-Spex-II-A) were also susceptible to Autographa californica multiple nucleopolyhedrovirus infection. In addition, they were characterized with regard to their growth rates and DNA amplification fingerprinting technique employing polymerase chain reaction.
Zhang
Huan
H
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datunlu, Beijing, 100101, China.
Zhang
Yong-an
YA
Qin
Qilian
Q
Li
Xuan
X
Miao
Lin
L
Wang
Yuzhu
Y
Qu
Liangjian
L
Zhang
Aijun
A
Yang
Qing
Q
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002999:Clone Cells; D004198:Disease Susceptibility; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000502:physiology*; D016133:Polymerase Chain Reaction; D018411:Spodoptera / Q000166:cytology* / Q000821:virology*