title: Genomic expression profiling of peripheral blood leukocytes of pigs infected with highly virulent classical swine fever virus strain Shimen.
abstract: Classical swine fever (CSF), caused by a virus of the same name (CSFV), is a highly contagious swine pyrexic disease featuring extensive haemorrhagic lesions and leukopenia, but little is known about the molecular mechanisms of its pathogenesis. To gain insight into the interaction between the virus and host cells, microarray analyses were performed to detect alterations in genomic expression of pig peripheral blood leukocytes (PBLs) following CSFV infection. Three healthy pigs were inoculated with a lethal dose of highly virulent CSFV strain Shimen. PBLs were isolated at the onset of typical clinical signs and total RNA was subjected to microarray analyses with Affymetrix Porcine Genome Array GeneChips. Of all 20,201 pig genes arrayed in the chip, 1745 showed altered expression (up- or downregulation) after infection. These were classified into eight functional groups, relating to cell proliferation (3.6%), immune response (2.1%), apoptosis (1.4%), kinase activity (1.4%), signal transduction (1.4%), transcription (0.7%), receptor activity (0.7%) and cytokines/chemokines (0.4%). The remaining 88.3% of genes had unknown functions. Alterations in genomic expression were confirmed by real-time RT-PCR of selected cellular genes and Western blotting of annexin 2, a cellular protein relating to virus infection. The observed expression changes of numerous genes involved in immune and inflammatory responses and in the apoptosis process indicate that CSFV has developed sophisticated mechanisms to cause leukopenia in infected pigs. These data provide a basis for exploring the molecular pathogenesis of CSFV infection through an understanding of the interaction between viral and cellular components.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 XiAn Da Road, Changchun 130062, PR China.|Institute of Veterinary Sciences, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China.
authors.forename: Zixue
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Institute of Veterinary Sciences, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China.
authors.forename: Jinfu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Institute of Veterinary Sciences, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China.
authors.forename: Huancheng
authors.initials: H
authors.lastname: Guo
authors.affiliation: Institute of Veterinary Sciences, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China.
authors.forename: Changchun
authors.initials: C
authors.lastname: Tu
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000502:physiology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D007962:Leukocytes / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013552:Swine