Pharmacokinetic analysis of glaucocalyxin A in rat plasma by liquid-liquid extraction and HPLC.
A validated HPLC method is described for determination of glaucocalyxin A in rat plasma using liquid-liquid extraction and UV absorbance detection. The extraction recovery of glaucocalyxin A ranged from 81.72 % to 79.25 %, the linear range was 0.2-10 microg/mL, and R was 0.9984. A pharmacokinetic analysis of glaucocalyxin A after intravenous administration in rats revealed that glaucocalyxin A followed a two-compartment open model. The values of t (1/2alpha) and t (1/2beta) were 4.327 min and 28.56 min, respectively, and the area under the plasma concentration-time curve (AUC) was 222.744 microg x min x mL (-1). The method developed was simple, rapid and specific, and an accurate way of investigating the pharmacokinetics of a diterpenoid in plasma.
Cao
Luye
L
Food and Pharmaceutics Vocational College of Guangdong, Guangzhou 510520, PR China. yncly001@yahoo.com.cn
Sun
Jin
J
Shen Li
Yun
Y
Chen
Zijun
Z
Li
Yikui
Y
D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D037602:Isodon / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar
D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C055124:glaucocalyxin A