Peroxo and oxo intermediates in mononuclear nonheme iron enzymes and related active sites.
Fe(III)OOH and Fe(IV)O intermediates have now been documented in a number of nonheme iron active sites. In this Current Opinion we use spectroscopy combined with electronic structure calculations to define the frontier molecular orbitals (FMOs) of these species and their contributions to reactivity. For the low-spin Fe(III)OOH species in activated bleomycin we show that the reactivity of this nonheme iron intermediate is very different from that of the analogous Compound 0 of cytochrome P450. For Fe(IV)O S=1 model species we experimentally define the electronic structure and its contribution to reactivity, and computationally evaluate how this would change for the Fe(IV)O S=2 intermediates found in nonheme iron enzymes.
Solomon
Edward I
EI
Department of Chemistry, Stanford University, CA 94305, United States. Edward.Solomon@stanford.edu
Wong
Shaun D
SD
Liu
Lei V
LV
Decker
Andrea
A
Chow
Marina S
MS
D020134:Catalytic Domain; D004789:Enzyme Activation; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D019159:Nonheme Iron Proteins / Q000737:chemistry* / Q000378:metabolism*; D011789:Quantum Theory; D013057:Spectrum Analysis
D004798:Enzymes; D019159:Nonheme Iron Proteins; D007501:Iron