Environmental proteomics: a paradigm shift in characterizing microbial activities at the molecular level.
The increase in sequencing capacity led to a new wave of metagenomic projects, enabling and setting the prerequisite for the application of environmental proteomics technologies. This review describes the current status of environmental proteomics. It describes sample preparation as well as the two major technologies applied within this field: two-dimensional electrophoresis-based environmental proteomics and liquid chromatography-mass spectrometry-based environmental proteomics. It also highlights current publications and describes major scientific findings. The review closes with a discussion of critical improvements in the area of integrating experimental mass spectrometry technologies with bioinformatics as well as improved sample handling.
Keller
Martin
M
Biosciences Division, Oak Ridge National Laboratory, One Bethel Valley Road, P.O. Box 2008, MS-6026, Oak Ridge, TN 37831, USA. kellerm@ornl.gov
Hettich
Robert
R
D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D019295:Computational Biology; D017753:Ecosystem; D004783:Environmental Microbiology; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D005658:Fungi / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods* / Q000639:trends*
D001426:Bacterial Proteins; D005656:Fungal Proteins; D020543:Proteome