[Effect of total flavones from Cuscuta chinensis on expression of Fas/FasL, PCNA and HB-EGF in SD rats model with bromocriptine-induced abortion].
OBJECTIVE
To explore the effect of total flavones from cuscuta chinensis (TFCC) on expression of Fas, PCNA and HB-EGF in SD rats model with bromocriptine-induced abortion.


METHODS
The model rats of bromocriptine during 6-8 d of pregnancy induced early abortion was established, adopting respectively herbs in high and low dosage and progesterone affect model rat and after 12 d, Immunohistochemical was applied to determine Fas, HB-EGF and PCNA in deciduas and placenta.


RESULTS
Expression of PCNA on trophoblast and deciduas, HB-EGF on trophoblast, PR on deciduas in the model used Semen cuscutae flavonoid, proesterone and normal pregnacy, were significantlly higher than those of the pure model. Expression of Fas on trophoblast and deciduas in above four groups, were significantlly lower than those of the pure model. There were no expression of HB-EGF on deciduas.


CONCLUSIONS
TFCC regulates the proliferation and apoptosis of the deciduas and cytotrophoblasts and prevents spontaneous abortions.
Ma
Hong-Xia
HX
The First Affiliated Hospital of Guangzhou Medicine College, Guangzhou 510120, China. doctormaxia1974@yahoo.com.cn
You
Zhao-Ling
ZL
Wang
Xiao-Yun
XY
D000022:Abortion, Spontaneous / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001971:Bromocriptine; D034182:Cuscuta / Q000737:chemistry*; D003656:Decidua / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism*; D005260:Female; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D066257:Heparin-binding EGF-like Growth Factor; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D010920:Placenta / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
D053222:Fas Ligand Protein; D047309:Flavones; C587885:Hbegf protein, rat; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D018809:Proliferating Cell Nuclear Antigen; D019014:fas Receptor; D001971:Bromocriptine