title: Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
abstract: Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPalpha, PPARgamma2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Taura
authors.forename: Michio
authors.initials: M
authors.lastname: Noguchi
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Sone
authors.forename: Kiminori
authors.initials: K
authors.lastname: Hosoda
authors.forename: Eisaku
authors.initials: E
authors.lastname: Mori
authors.forename: Yohei
authors.initials: Y
authors.lastname: Okada
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Homma
authors.forename: Naofumi
authors.initials: N
authors.lastname: Oyamada
authors.forename: Megumi
authors.initials: M
authors.lastname: Inuzuka
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Sonoyama
authors.forename: Ken
authors.initials: K
authors.lastname: Ebihara
authors.forename: Naohisa
authors.initials: N
authors.lastname: Tamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Suemori
authors.forename: Norio
authors.initials: N
authors.lastname: Nakatsuji
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamanaka
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D000071317:Nanog Homeobox Protein; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D039904:Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
chemical_list: D015415:Biomarkers; D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D005353:Fibronectins; D018398:Homeodomain Proteins; D020738:Leptin; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D047495:PPAR gamma; D010455:Peptides; C008973:polyornithine