Enantiorecognition of profens by capillary electrophoresis using a novel chiral selector eremomycin.
The evaluation of a macrocyclic glycopeptide antibiotic, eremomycin, as a chiral selector in capillary electrophoresis (CE) has been performed. The stability of eremomycin in solution and capillary electrolyte, as well as its optical and electrophoretic properties have been discussed. The effect of experimental parameters influencing the enantioseparation of several profens has been studied. Excellent enantioseparation of profens has been achieved and migration order has been validated. Comparison of enantioseparations of profens in CE by using eremomycin-mediated electrolytes and in HPLC with eremomycin immobilized on silica has revealed similar trends for both methods.
Prokhorova
Aleksandra F
AF
Chemistry Department, Division of Analytical Chemistry, Lomonosov Moscow State University, Leninskie Gory, Moscow, Russian Federation.
Shapovalova
Elena N
EN
Shpak
Aleksei V
AV
Staroverov
Sergei M
SM
Shpigun
Oleg A
OA
D002851:Chromatography, High Pressure Liquid; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005480:Flurbiprofen / Q000032:analysis*; D006020:Glycopeptides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010666:Phenylpropionates / Q000032:analysis*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism
D004573:Electrolytes; D006020:Glycopeptides; D010666:Phenylpropionates; D012997:Solvents; C053759:eremomycin; D005480:Flurbiprofen