Self-assembly of a four-helix bundle on a DNA quadruplex.
Come together: A novel method for assembling monomers and controlling structure of a de novo helix bundle protein is described. A guanine (G)-rich oligodeoxynucleotide scaffold forms a hydrogen-bonded DNA quadruplex in the presence of potassium counterions, thereby inducing a helical structure and fourfold stoichiometry in conjugated, amphiphilic peptide sequences. The DNA scaffold shows potential for rapidly assembling designed proteins.
Rosenzweig
Brooke A
BA
Department of Chemistry, Yale University, P.O. Box 208017, New Haven, CT 06520-8107, USA.
Hamilton
Andrew D
AD
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D054856:G-Quadruplexes; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011188:Potassium / Q000737:chemistry; D017510:Protein Folding; D017433:Protein Structure, Secondary
D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011188:Potassium