title: Determination of Helicobacter pylori virulence by analysis of the cag pathogenicity island isolated from Iranian patients.
abstract: BACKGROUND
  The cag pathogenicity island (PAI), which can be divided into two parts, cagI and cagII, is the most well-known virulence factor of Helicobacter pylori.
  
  
  OBJECTIVE
  We investigated the association between genetic variations within the cag PAI (cagA and cagE in the cagI and cagT in the cagII) and clinical outcomes in Iranian patients.
  
  
  METHODS
  A total of 231 patients including 182 patients with gastritis, 41 with peptic ulcer and 8 with gastric cancer.
  
  
  METHODS
  The presence of the cagA, cagE and cagT genes were measured by polymerase chain reaction and the results were compared with clinical outcomes and gastric histology.
  
  
  RESULTS
  The cagA, cagE and cagT genes were found in 154 (66.7%), 90 (39.0%) and 70 (30.3%) of clinical isolates. At least 144 (62.3%) strains possessed partially deleted cag PAI (e.g., 69 [29.9%] strains were cagA-positive, but cagE and cagT-negative).
  
  
  CONCLUSIONS
  The single genes as well as the combination of genes in the cag PAI appeared not to be useful markers to predict H. pylori-related diseases in Iranian patients. The genomic sequences of the cag PAI in Iranian strains might be considerably different from those in other geographic locations.
authors.affiliation: Research Center of Gastroenterology and Liver Diseases in Shahid Beheshti University, Tehran, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Baghaei
authors.forename: L
authors.initials: L
authors.lastname: Shokrzadeh
authors.forename: F
authors.initials: F
authors.lastname: Jafari
authors.forename: H
authors.initials: H
authors.lastname: Dabiri
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: M
authors.initials: M
authors.lastname: Bolfion
authors.forename: H
authors.initials: H
authors.lastname: Zojaji
authors.forename: M M
authors.initials: MM
authors.lastname: Aslani
authors.forename: M R
authors.initials: MR
authors.lastname: Zali
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D005260:Female; D005756:Gastritis / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics*; D016481:Helicobacter Infections / Q000235:genetics; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D037521:Virulence Factors / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C103937:PicB protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori