title: Construction of cDNA libraries: focus on protists and fungi.
abstract: Sequencing of cDNA libraries is an efficient and inexpensive approach to analyze the protein-coding portion of a genome. It is frequently used for surveying the genomes of poorly studied eukaryotes, and is particularly useful for species that are not easily amenable to genome sequencing, because they are nonaxenic and/or difficult to cultivate. In this chapter, we describe protocols that have been applied successfully to construct and normalize a variety of cDNA libraries from many different species of free-living protists and fungi, and that require only small quantities of cell material.
authors.affiliation: Robert-Cedergren Centre for Bioinformatics and Genomics, Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Naiara
authors.initials: N
authors.lastname: Rodríguez-Ezpeleta
authors.forename: Shona
authors.initials: S
authors.lastname: Teijeiro
authors.forename: Lise
authors.initials: L
authors.lastname: Forget
authors.forename: Gertraud
authors.initials: G
authors.lastname: Burger
authors.forename: B Franz
authors.initials: BF
authors.lastname: Lang
mesh_terms: D018076:DNA, Complementary / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005658:Fungi / Q000235:genetics*; D015723:Gene Library; D005821:Genetic Techniques; D023281:Genomics; D012313:RNA / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012313:RNA; D012194:RNA-Directed DNA Polymerase