Rapid screening platform for stabilization of scFvs in Escherichia coli.
The poor biophysical properties of antibody fragments such as scFvs and diabodies can preclude their use as therapeutic agents. The non-ideal biophysical properties and insufficient thermal stability of antibody fragments often leads to poor expression, poor solubility, and a predisposition of the proteins to aggregate. We have developed a general platform for engineering stability into antibody fragments. By promoting Escherichia coli cultures to secrete scFvs directly into growth media, automated screening methods can be applied to empirically evaluate multiple stability design strategies including rational, sequence-based, and structure-based designs. Once stabilized, these antibody fragments demonstrate improved expression and durability during purification, handling, and storage. Stabilized antibody fragments can also be used as building blocks for multivalent or bispecific antibody-like molecules.
Miller
Brian R
BR
Biogen Idec, San Diego, CA, USA.
Glaser
Scott M
SM
Demarest
Stephen J
SJ
D004926:Escherichia coli / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D009154:Mutation / Q000235:genetics; D019151:Peptide Library; D055550:Protein Stability; D015203:Reproducibility of Results
D007135:Immunoglobulin Variable Region; D019151:Peptide Library