title: Inflammatory and bone-related genes are modulated by aging in human periodontal ligament cells.
abstract: Periodontal ligament cells (PDLC) play a major role in periodontal tissues homeostasis and destruction. Most age-associated diseases seem to be closely related to an underlying chronic inflammatory state. Thus, the present study aimed at evaluating in PDLC the effect of aging on the basal levels of inflammatory and bone-related genes. Primary PDLC cultures were obtained from subjects aged 15-20 years (control- n=5), and subjects aged more than 60 years (test- n=5). Proliferation, cell viability and total secreted protein assays were performed, and mRNA levels were quantitatively assessed for interleukin (IL)-1beta, IL-4, IL-6 and IL-8, and for receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPG) by real time PCR. Data analysis demonstrated that aging negatively influenced cell proliferation, whereas cell viability and total secreted protein were not affected (p>0.05). Gene expression analysis showed that mRNA levels for RANKL and IL-8 were not affected by aging (p>0.05) whereas, mRNA levels for IL-4 was significantly lower in aged cells (p<0.05) and OPG, IL-1beta and IL-6 mRNA levels were higher (p<0.05). Data analysis suggests that aging decreased the ability of PDLC to proliferate and modulated the expression of important inflammatory and bone-related genes in periodontal ligament cells, favoring a proinflammatory and an antiresorptive profile.
authors.affiliation: School of Dentistry, Federal University of Maranhão, Av. dos Portugueses, Campus Bacanga, CEP 65085-580, São Luís, Maranhão, Brazil.
authors.forename: Bruno Braga
authors.initials: BB
authors.lastname: Benatti
authors.forename: Karina Gonzales
authors.initials: KG
authors.lastname: Silvério
authors.forename: Márcio Zaffalon
authors.initials: MZ
authors.lastname: Casati
authors.forename: Enilson Antônio
authors.initials: EA
authors.lastname: Sallum
authors.forename: Francisco Humberto
authors.initials: FH
authors.lastname: Nociti
mesh_terms: D000293:Adolescent; D000368:Aged; D000375:Aging / Q000235:genetics*; D001842:Bone and Bones / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D015847:Interleukin-4