title: A4D12 monoclonal antibody recognizes a new linear epitope from SAG2A Toxoplasma gondii tachyzoites, identified by phage display bioselection.
abstract: Toxoplasma gondii surface is coated by closely related antigens that belong to SRS (SAG-1 related sequences) superfamily. Two tachyzoite-specific SRS antigens, SAG1 and SAG2, are immunodominant proteins that apparently modulate the virulence of infection by inducing the host immune response against tachyzoites during the acute phase. In this study, we described a conformationally insensitive monoclonal antibody (A4D12mAb) that recognizes a linear epitope shared by two isoforms of p22 that is expressed in the surface of T. gondii tachyzoites. By using phage display approach and production of recombinant proteins, we clearly demonstrated that the A4D12mAb recognizes an epitope within C-terminal region of SAG2A. This mAb reacts with both T. gondii genotypes (I and II) but not with a closely related parasite, Neospora caninum. Also, the pretreatment of tachyzoites with A4D12 mAb did not inhibit T. gondii infection, suggesting that the epitope herein mapped is not crucial for tachyzoite invasion. However, a panel of human T. gondii positive sera showed significant degree of inhibition of A4D12 mAb reactivity against T. gondii native antigens, indicating that both A4D12 mAb and human sera recognize an overlapping immunodominant epitope within C-terminal region of SAG2A. To our knowledge, this is the first evidence using bioselection by phage display that identifies a T. gondii linear epitope recognized by a mAb specific to SAG2A. In conclusion, the results here presented add a new piece of information concerning T. gondii SAG2A molecule, emphasizing two dissimilar biological roles of this molecule, particularly for A4D12 epitope, suggesting that these characteristics may be important for parasite survival, since it is part of parasite components able to induce a strong immune response enough to allow host survival and establish long-term chronic infection.
authors.affiliation: Institute of Biomedical Sciences, Federal University of Uberlândia, Brazil.
authors.forename: Jair P
authors.initials: JP
authors.lastname: Cunha-Júnior
authors.forename: Deise A O
authors.initials: DA
authors.lastname: Silva
authors.forename: Neide M
authors.initials: NM
authors.lastname: Silva
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souza
authors.forename: Guilherme R L
authors.initials: GR
authors.lastname: Souza
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Prudencio
authors.forename: Carlos P
authors.initials: CP
authors.lastname: Pirovani
authors.forename: Júlio
authors.initials: J
authors.lastname: Cezar M Cascardo
authors.forename: Bellisa F
authors.initials: BF
authors.lastname: Barbosa
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Goulart
authors.forename: José R
authors.initials: JR
authors.lastname: Mineo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular; D018604:Epitope Mapping; D005347:Fibroblasts / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D006801:Humans; D006825:Hybridomas; D007106:Immune Sera; D056724:Immunity, Humoral; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000276:immunology; D019151:Peptide Library; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D014123:Toxoplasmosis / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D014774:Virulence
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D016056:Immunodominant Epitopes; D019151:Peptide Library; D015800:Protozoan Proteins; C067547:surface antigen P22, Toxoplasma