title: Contaminated soils (II): in vitro dermal absorption of nickel (Ni-63) and mercury (Hg-203) in human skin.
abstract: Dermal absorption of heavy metal soil contaminants was tested in vitro with chloride salts of radioactive nickel (Ni-63) and mercury (Hg-203). Aqueous soil suspensions, spiked with either Ni-63 or Hg-203, were applied to fresh viable human breast skin tissue in Bronaugh diffusion cells perfused with Hanks HEPES buffered (pH 7.4) receptor containing 4% bovine serum albumin (BSA). Receptor fractions were collected every 6 h for 24 h when skin was soap washed. Tests were conducted concurrently in triplicate with and without soil for each skin specimen. Mean percent dermal absorption including the skin depot for Ni-63 was 1 and 22.8% with and without soil, respectively, while for Hg-203, values of 46.6 and 78.3% were obtained. Excluding the skin depot and considering only absorption in receptor, there was 0.5 and 1.8% absorption of Ni-63 with and without soil, respectively, and 1.5 and 1.4% for Hg-203. The potential bioavailability of the skin depot is discussed in relation to dermal exposure to these metals in contaminated soil.
authors.affiliation: Exposure and Bio-Monitoring Division, Dermal Absorption Lab Unit, Health Canada, Ottawa ONK1AOK9, Canada. rick_moody@hc-sc.gc.ca
authors.forename: Richard P
authors.initials: RP
authors.lastname: Moody
authors.forename: Julie
authors.initials: J
authors.lastname: Joncas
authors.forename: Mark
authors.initials: M
authors.lastname: Richardson
authors.forename: Sanya
authors.initials: S
authors.lastname: Petrovic
authors.forename: Ih
authors.initials: I
authors.lastname: Chu
mesh_terms: D000383:Agriculture; D016477:Artifacts; D001682:Biological Availability; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008628:Mercury / Q000493:pharmacokinetics*; D008631:Mercury Radioisotopes / Q000493:pharmacokinetics; D009532:Nickel / Q000493:pharmacokinetics*; D011868:Radioisotopes / Q000493:pharmacokinetics; D012869:Skin Absorption; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000493:pharmacokinetics*
chemical_list: D008631:Mercury Radioisotopes; D011868:Radioisotopes; D012987:Soil; D012989:Soil Pollutants; D009532:Nickel; D008628:Mercury