Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.
Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 microM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.
Bae
Sungwoo
S
Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
Wuertz
Stefan
S
D001386:Azides / Q000378:metabolism / Q000494:pharmacology*; D001438:Bacteroidaceae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005456:Fluorescent Dyes / Q000378:metabolism / Q000494:pharmacology*; D050296:Microbial Viability; D016133:Polymerase Chain Reaction / Q000379:methods*; D011419:Propidium / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013170:Spores / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014871:Water Microbiology
D001386:Azides; D005456:Fluorescent Dyes; C533957:propidium monoazide; D011419:Propidium; D004247:DNA