[AQP1 expression in vascular endothelial cells in vitro under hypoxia conditions].
OBJECTIVE
To investigate the expression of AQP1 in endothelial cells under hypoxia conditions in vitro.


METHODS
The cultured human umbilical vein endothelial cells were randomly assigned to hypoxia groups (exposed to 1% of O2 for 3 h, 6 h, 12 h and 24 h respectively), and control group (ordinary culture). The expressions of AQP1 mRNA and protein were detected by real time PCR and immunohistochemistry respectively.


RESULTS
The expressions of AQP1 protein and mRNA increased after exposure to hypoxia initially and then decreased afterwards.


CONCLUSIONS
Hypoxia regulates the expression of AQP1 in vascular endothelial cells.
Wu
Qin-qin
QQ
Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
Yu-cheng
YC
Jiang
Xiao-fei
XF
Zeng
Zhi
Z
D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D014471:Umbilical Veins / Q000166:cytology*
C496020:AQP1 protein, human; D051398:Aquaporin 1