title: Contribution of methylated exudate flavonoids to the anti-inflammatory activity of Grindelia robusta.
abstract: The flavonoid pattern of an acetonic extract of Grindelia robusta Nutt. was investigated in detail. The flavonoids were enriched by CC. In addition to twelve known methylated exudate flavonols four compounds were identified for the first time in G. robusta. Several substances of the flavonoid complex, among them the main compounds quercetin-3-methylether and 6-OH-kaempferol-3,6-dimethylether, were tested for their activity to inhibit neutrophil elastase. Quercetin-3-methylether was shown to be most active with an IC(50) of 19 microM, thus obviously contributing to the anti-inflammatory activity of the drug.
authors.affiliation: Department of Pharmacognosy, University of Vienna, PharmaCenter Vienna, Vienna, Austria. liselotte.krenn@univie.ac.at
authors.forename: Liselotte
authors.initials: L
authors.lastname: Krenn
authors.forename: Eckhard
authors.initials: E
authors.lastname: Wollenweber
authors.forename: Katja
authors.initials: K
authors.lastname: Steyrleuthner
authors.forename: Cornelia
authors.initials: C
authors.lastname: Görick
authors.forename: Matthias F
authors.initials: MF
authors.lastname: Melzig
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D044948:Flavonols / Q000302:isolation & purification* / Q000494:pharmacology; D036623:Grindelia / Q000737:chemistry*; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives
chemical_list: C543233:6-OH-kaempferol-3,6-dimethylether; D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D044948:Flavonols; D010936:Plant Extracts; C459210:quercetin 3-O-methyl ether; D011794:Quercetin; D019272:Leukocyte Elastase