Molecular interactions between barley and oat beta-glucans and phenolic derivatives.
Equilibrium dialysis, molecular modeling, and multivariate data analysis were used to investigate the nature of the molecular interactions between 21 vanillin-inspired phenolic derivatives, 4 bile salts, and 2 commercially available beta-glucan preparations, Glucagel and PromOat, from barley and oats. The two beta-glucan products showed very similar binding properties. It was demonstrated that the two beta-glucan products are able to absorb most phenolic derivatives at a level corresponding to the absorption of bile salts. Glucosides of the phenolic compounds showed poor or no absorption. The four phenolic derivatives that showed strongest retention in the dialysis assay shared the presence of a hydroxyl group in para-position to a CHO group. However, other compounds with the same structural feature but possessing a different set of additional functional groups showed less retention. Principal component analysis (PCA) and partial least-squares regression (PLS) calculations using a multitude of diverse descriptors related to electronic, geometrical, constitutional, hybrid, and topological features of the phenolic compounds showed a marked distinction between aglycon, glucosides, and bile salt retention. These analyses did not offer additional information with respect to the mode of interaction of the individual phenolics with the beta-glucans. When the barley beta-glucan was subjected to enzyme degradation, the ability to bind some but not all of the phenolic derivatives was lost. It is concluded that the binding must be dependent on multiple characteristics that are not captured by a single molecular descriptor.
Simonsen
Henrik Toft
HT
Plant Biochemistry Laboratory and Laboratory for Molecular Plant Biology, VKR Research Centre Pro-Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark.
Nielsen
Mette S
MS
Christensen
Niels J
NJ
Christensen
Ulla
U
La Cour
Thomas V
TV
Motawia
Mohammed Saddik
MS
Jespersen
Birthe P M
BP
Engelsen
Søren B
SB
Møller
Birger Lindberg
BL
D018554:Avena / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000737:chemistry; D001467:Hordeum / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry*
D001647:Bile Acids and Salts; D010636:Phenols; D047071:beta-Glucans