[Poly-aspartamide-glutamic acid grafted low molecular weight polyethylenimine as a novel non-viral gene vector].
To develop a novel gene delivery vector with poly-aspartamide-glutamic acid and polyethylenimine as the backbone.



alpha, beta-poly-(N-2-hydroxypropyl)-D, L-aspartamide-glutamic acid (PHPAG) was synthesized and low molecular weight polyethylenimine (PEI 1.8 kDa) was grafted to form PHPAG-PEI 1800. Chemical and biological characterization of the polymer was identified.



The polymer was confirmed by (1)H-NMR, and the molecular weight was about 1.2 x 10(4). The ability of DNA binding was showed by gel retardation assay at N/P ratio of 3. 5. MTT assay showed that the polymer was non toxic in COS-7 and A293 cell lines. In vitro test demonstrated that it had high transfection efficiency in B16 and Hela cell lines.



PHPAG-PEI 1800 was successfully synthesized,which might be a potential vector for gene delivery.
Chen
Dan
D
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
He
Bin-Bin
BB
Zhao
Dan-Jun
DJ
Jiang
Qi-Ying
QY
Wang
Zhong-Ren
ZR
Zhou
Jun
J
Yu
Hai
H
Wang
Qing-Qing
QQ
Tang
Gu-Ping
GP
D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D018698:Glutamic Acid / Q000737:chemistry*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C071943:polyaspartylglutamate; C017645:polyaspartate; D018698:Glutamic Acid; D011094:Polyethyleneimine