[Association of apolipoprotein A5 gene -1131T/C polymorphism with serum lipids and carotid intima-media thickness in patients with type 2 diabetes mellitus].
OBJECTIVE
To investigate the relationship between apolipoprotein A5 gene -1131T/C polymorphism and serum lipids levels and carotid intima-media thickness in patients with type 2 diabetes mellitus in a Chinese population in Chengdu.


METHODS
The genotype and allele frequencies of apolipoprotein A5-1131T/C polymorphism were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide electrophoresis (PAGE) methods. The serum lipids were measured with enzymatic kits in 154 type 2 diabetic patients and 206 normal people (control). The carotid intima-media thickness (IMT) of 116 type 2 diabetic patients was measured by B-mode ultrasonography.


RESULTS
The allele frequencies of T, C in the diabetic patients were 0.721, 0.279, respectively, which were not significantly different from those of the normal controls (0. 760, 0. 240). Compared with the wild type TT, CC homozygote increased the risk of type 2 diabetes significantly (OR=2.432, 95% CI: 1.081-5.473). In the patients with type 2 diabetes, the serum triglyceride (TG) level and TG/HDL-C ratio were greater in those with TC and CC genotypes than those with TT genotype subjects (P<0.05). The normal people with TC genotype also had greater triglyceride levels and TG/HDL-C ratio than those with TT genotype. The diabetic patients with CC genotype had greater carotid IMT than those with TT genotype (P=0.08).


CONCLUSIONS
The -1131T/C polymorphism in the apolipoprotein A5 gene may have an impact on serum triglyceride levels and TG/HDL-C ratio. People with homozygote CC have increased risk of type 2 diabetes. But more evidence is needed to prove its association with carotid IMT in patients with type 2 diabetes.
Qiao
Yan
Y
Division of Peptides Related with Human Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Rui
R
Tian
Hao-ming
HM
Liu
Yu
Y
Qiang
Ou
O
Liu
Bing-wen
BW
D000328:Adult; D000368:Aged; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D002339:Carotid Arteries / Q000000981:diagnostic imaging / Q000473:pathology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017539:Tunica Intima / Q000000981:diagnostic imaging / Q000473:pathology; D014463:Ultrasonography
C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D008055:Lipids