title: Simplified procedures for the isolation of HF3, bothropasin, disintegrin-like/cysteine-rich protein and a novel P-I metalloproteinase from Bothrops jararaca venom.
abstract: HF3 and bothropasin are P-III hemorrhagic snake venom metalloproteinases (SVMPs) of Bothrops jararaca. The DC protein is composed of the disintegrin-like/cysteine-rich domains derived from the autolysis of P-III SVMPs. Here we describe simplified procedures for the isolation of HF3, bothropasin, the DC protein, and BJ-PI, a novel P-I SVMP. The isolated proteins were identified by mass spectrometry. BJ-PI is a potent caseinolytic enzyme devoid of hemorrhagic activity. HF3, bothropasin and BJ-PI show distinct fibrinogenolytic activities.
authors.affiliation: Laboratório Especial de Toxinologia Aplicada-CAT-CEPID, Instituto Butantan, São Paulo, Brazil.
authors.forename: Ana K
authors.initials: AK
authors.lastname: Oliveira
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
authors.forename: Marina T
authors.initials: MT
authors.lastname: Assakura
authors.forename: Milene C
authors.initials: MC
authors.lastname: Menezes
authors.forename: André
authors.initials: A
authors.lastname: Zelanis
authors.forename: Alexandre K
authors.initials: AK
authors.lastname: Tashima
authors.forename: Mônica
authors.initials: M
authors.lastname: Lopes-Ferreira
authors.forename: Carla
authors.initials: C
authors.lastname: Lima
authors.forename: Antonio C M
authors.initials: AC
authors.lastname: Camargo
authors.forename: Jay W
authors.initials: JW
authors.lastname: Fox
authors.forename: Solange M T
authors.initials: SM
authors.lastname: Serrano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017837:Bothrops / Q000502:physiology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D006470:Hemorrhage / Q000139:chemically induced / Q000473:pathology; D008666:Metalloendopeptidases / Q000302:isolation & purification* / Q000633:toxicity; D045726:Metalloproteases / Q000302:isolation & purification* / Q000633:toxicity; D051379:Mice; D008969:Molecular Sequence Data; D014357:Trypsin / Q000737:chemistry; D014757:Viper Venoms / Q000201:enzymology*
chemical_list: D003435:Crotalid Venoms; D014757:Viper Venoms; D045726:Metalloproteases; D014357:Trypsin; C543527:HF3 protein, Bothrops jararaca; D008666:Metalloendopeptidases; C472493:bothropasin, Bothrops jararaca