Microleakage of human saliva in coronally unsealed obturated root canals in anaerobic conditions.
The purpose of this study was to determine the time required for anaerobic bacteria in natural human saliva to contaminate root-filled teeth. Thirty-two single-rooted teeth were cleaned, shaped, filled, and exposed to human saliva for 120 days. Teeth that had not leaked were subjected to polymerise chain reaction examination. Sixty-six percent of the experimental group were totally contaminated. A PCR examination revealed there was no contamination in the apical 3 mm of leakage-free teeth.
Yazdi
K Ashofteh
KA
Department of Endodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Iran.
Bayat-Movahed
S
S
Aligholi
M
M
Hayes
S J
SJ
Nekoofar
M H
MH
D001421:Bacteria, Anaerobic / Q000145:classification / Q000302:isolation & purification*; D001729:Bismuth / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000032:analysis; D003763:Dental Leakage / Q000145:classification / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D004587:Electrophoresis, Agar Gel; D004853:Epoxy Resins / Q000627:therapeutic use; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D016133:Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012389:Root Canal Obturation / Q000295:instrumentation / Q000379:methods*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D012463:Saliva / Q000382:microbiology*; D012834:Silver / Q000627:therapeutic use; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000627:therapeutic use; D019227:Tooth Apex / Q000382:microbiology
D004269:DNA, Bacterial; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D012834:Silver; C028044:epoxy resin AH-26; D006171:Gutta-Percha; D014025:Titanium; D012973:Sodium Hypochlorite; D001729:Bismuth