Capillary electrophoresis with contactless conductivity detection for uric acid determination in biological fluids.
The suitability of capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C(4)D) for the direct determination of uric acid in human plasma and urine was investigated. It was found that a careful optimization of the buffer composition and pH was necessary to achieve selective determination in the complex sample matrices. An electrolyte solution consisting of 10mM 2-morpholinoethanesulfonic acid (MES), 10mM histidine and 0.1mM hexadecyltrimethylammonium bromide (CTAB), pH 6.0, was finally found suitable for use as running buffer for both sample matrices. The limit of detection (3 S/N) was determined as 3.3 microM. The linearity of the response was tested for the range between 10 and 500 microM and a correlation coefficient of 0.9996 was obtained. Intra- and inter-day variabilities were <10%. Quantitative analysis of urine and plasma samples showed a good correlation with the routine enzymatic method currently used at the University Hospital of Basel.
Pormsila
Worapan
W
Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
Krähenbühl
Stephan
S
Hauser
Peter C
PC
D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine
D012997:Solvents; D014527:Uric Acid