title: Overexpression of acetyl-CoA carboxylase gene of Mucor rouxii enhanced fatty acid content in Hansenula polymorpha.
abstract: Malonyl-CoA is an essential precursor for fatty acid biosynthesis that is generated from the carboxylation of acetyl-CoA. In this work, a gene coding for acetyl-CoA carboxylase (ACC) was isolated from an oleaginous fungus, Mucor rouxii. According to the amino acid sequence homology and the conserved structural organization of the biotin carboxylase, biotin carboxyl carrier protein, and carboxyl transferase domains, the cloned gene was characterized as a multi-domain ACC1 protein. Interestingly, a 40% increase in the total fatty acid content of the non-oleaginous yeast Hansenula polymorpha was achieved by overexpressing the M. rouxii ACC1. This result demonstrated a significant improvement in the production of fatty acids through genetic modification in this yeast strain.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Rawisara
authors.initials: R
authors.lastname: Ruenwai
authors.forename: Supapon
authors.initials: S
authors.lastname: Cheevadhanarak
authors.forename: Kobkul
authors.initials: K
authors.lastname: Laoteng
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005227:Fatty Acids / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005990:Glycerol / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D005227:Fatty Acids; D005656:Fungal Proteins; D000103:Acetyl-CoA Carboxylase; D005990:Glycerol; D000432:Methanol