title: Transcriptional responses of Mycobacterium tuberculosis to lung surfactant.
abstract: This study uses microarray analyses to examine gene expression profiles for Mycobacterium tuberculosis (Mtb) induced by exposure in vitro to bovine lung surfactant preparations that vary in apoprotein content: (i) whole lung surfactant (WLS) containing the complete mixture of endogenous lipids and surfactant proteins (SP)-A, -B, -C, and -D; (ii) extracted lung surfactant (CLSE) containing lipids plus SP-B and -C; (iii) column-purified surfactant lipids (PPL) containing no apoproteins, and (iv) purified human SP-A. Exposure to WLS evoked a multitude of transcriptional responses in Mtb, with 52 genes up-regulated and 23 genes down-regulated at 30min exposure, plus 146 genes up-regulated and 27 genes down-regulated at 2h. Notably, WLS rapidly induced several membrane-associated lipases that presumptively act on surfactant lipids as substrates, and a large number of genes involved in the synthesis of phthiocerol dimycocerosate (PDIM), a cell wall component known to be important in macrophage interactions and Mtb virulence. Exposure of Mtb to CLSE, PPL, or purified SP-A caused a substantially weaker transcriptional response (</=20 genes were induced) suggesting that interactions among multiple lipid-protein components of WLS may contribute to its effects on Mtb transcription.
authors.affiliation: Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA. ues3@cornell.edu
authors.forename: Ute
authors.initials: U
authors.lastname: Schwab
authors.forename: Kyle H
authors.initials: KH
authors.lastname: Rohde
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Wang
authors.forename: Patricia R
authors.initials: PR
authors.lastname: Chess
authors.forename: Robert H
authors.initials: RH
authors.lastname: Notter
authors.forename: David G
authors.initials: DG
authors.lastname: Russell
mesh_terms: D000818:Animals; D002417:Cattle; D020869:Gene Expression Profiling; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D011663:Pulmonary Surfactants / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D011663:Pulmonary Surfactants