title: Glutathione s-transferase variants in a brazilian population.
abstract: Recent pharmacogenomic studies have revealed significant interethnic differences in glutathione S-transferase (GST) allelic frequencies among various ethnic groups. Therefore, we have investigated GSTM1 (gene deletion), GSTT1 (gene deletion) and GSTP1 (rs1695) polymorphism frequencies in 3 Brazilian ethnic groups (n = 203). GSTM1 and GSTT1 polymorphism analyses were performed by multiplex polymerase chain reaction, and GSTP1 (rs1695) analysis was done by polymerase chain reaction restriction fragment length polymorphism. GSTM1- polymorphism frequency was 33.2%, while GSTT1 null (GSTT1-) was 30.2%. The valine GSTP1*B (rs1695) allele was present in 35.1% subjects, while the heterozygous form (isoleucine/valine) was the most prevalent genotype (46.6%). We found a statistically significant difference in genotype frequency among Amerindians versus Caucasians (p = 0.016) and among Amerindians versus African-Americans (p = 0.033). Considerable frequency variation was found in our study, even when compared with other studies showing phylogeographical heterogeneity to the genes studied in Brazilian populations.
authors.affiliation: Laboratório de Farmacogenômica e Epidemiologia Molecular, Universidade Estadual de Santa Cruz, Ilhéus, Brazil.
authors.forename: Luiz Alexandre V
authors.initials: LA
authors.lastname: Magno
authors.forename: Jhimmy
authors.initials: J
authors.lastname: Talbot
authors.forename: Teddy
authors.initials: T
authors.lastname: Talbot
authors.forename: Alex Marques
authors.initials: AM
authors.lastname: Borges Santos
authors.forename: Renan P
authors.initials: RP
authors.lastname: Souza
authors.forename: Lauro J
authors.initials: LJ
authors.lastname: Marin
authors.forename: Marcos Lázaro
authors.initials: ML
authors.lastname: Moreli
authors.forename: Paulo R S
authors.initials: PR
authors.lastname: de Melo
authors.forename: Ronan X
authors.initials: RX
authors.lastname: Corrêa
authors.forename: Fabrício
authors.initials: F
authors.lastname: Rios Santos
authors.forename: Giuliano
authors.initials: G
authors.lastname: Di Pietro
mesh_terms: D000328:Adult; D001741:Black or African American / Q000235:genetics; D001938:Brazil / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007199:Indians, South American / Q000235:genetics; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D044465:White People / Q000235:genetics
chemical_list: D005982:Glutathione Transferase; C413545:glutathione S-transferase T1