A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer.
OBJECTIVE
With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer.


METHODS
GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach.


RESULTS
Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90.


CONCLUSIONS
These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
Chen
Yuxin
Y
Lab of Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, 210029 Nanjing, Jiangsu, People's Republic of China.
Zhao
Muzi
M
Wang
Saiqun
S
Chen
Jie
J
Wang
Yun
Y
Cao
Qinhong
Q
Zhou
Wenbin
W
Liu
Jin
J
Xu
Zhiyang
Z
Tong
Guoqing
G
Li
Jianmin
J
D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D015854:Up-Regulation / Q000235:genetics
D003598:Cytoskeletal Proteins; C478878:DNAAF4 protein, human; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger