Hybrid of baculovirus and galactosylated PEI for efficient gene carrier.
Baculovirus, containing an appropriate eukaryotic promoter, is considered an attractive approach for an efficient and safe gene delivery vehicle. However, the drawbacks of baculovirus, such as the lack of specificity and the inactivation of baculovirus by the complement system in human serum, negatively affect efficient gene delivery. Therefore, a hybrid system utilizing the positive aspects of both viral and non-viral vector systems would be useful to overcome the obstacles of either system alone. In this study, we constructed a hybrid system composed of baculovirus (B) and galactosylated polyethylenimine (GP)/DNA complexes through electrostatic interaction. The resulting GP/B hybrid had suitable physicochemical properties and low cytotoxicity for use in gene therapy. Furthermore, the GP/B significantly enhanced transduction efficiency and showed good cell-specificity compared to either viral or non-viral vector systems. These results suggest that the GP/B hybrid system can be used in gene therapy to enhance transduction efficiency and hepatocyte specificity.
Kim
You-Kyoung
YK
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Choi
Jae Young
JY
Jiang
Hu-Lin
HL
Arote
Rohidas
R
Jere
Dhananjay
D
Cho
Myung-Haing
MH
Je
Yeon Ho
YH
Cho
Chong-Su
CS
D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D048271:Chitosan / Q000031:analogs & derivatives* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000378:metabolism; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
D004337:Drug Carriers; C534328:galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine; D011094:Polyethyleneimine; D048271:Chitosan