Experimental study on early protective effect of ischemic preconditioning on rat kidney graft.
OBJECTIVE
Our aim was to investigate the mechanisms of early protection by ischemic preconditioning (IPC) of transplanted rat kidneys.


METHODS
Thirty-six male donor and recipient Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham-operated (A), transplantation (B), and IPC treatment (C) groups. Group A underwent exploratory laparotomy, group B received orthotopic transplantation, and group C, 15 minutes of ischemia followed by 10 minutes of reperfusion before transplantation. We measured the serum creatinine (SCr), blood urea nitrogen (BUN), and degree of kidney graft ischemic/reperfusion injury (IRI) by tumor necrosis factor-alpha (TNF-alpha) IkappaB kinase beta (IKK-beta) nuclear factor-kappa beta (NF-kappabeta) p65 subunit mRNA expressions.


RESULTS
The SCr and BUN levels in groups C and B were higher than those in the sham-operated group (P < .01), without a significant difference between groups C and B at 24 hours after transplantation (P > .05). The degree of renal graft tubular injury in group C was significantly lower than that in group B (P < .01). TNF-alpha transcription levels at 24 hours after transplantation were significantly lower compared with the non-IPC group (P < .01). However, we failed to note a significant difference in IKK-beta or p65 mRNA expressions between groups C and B (P > .05).


CONCLUSIONS
One cycle of preconditioning (15 min/10 min) attenuated renal graft IRI in the early phase. The inhibiting effects on TNF-alpha and the positive feedback signaling of TNF-alpha/NF-KB pathways may play important roles in renal graft protection.
Jia
R P
RP
Nanjing Medical University, Department of Urology, Center of Renal Transplantation, Jiangsu, China.
Zhu
J G
JG
Wu
J P
JP
Xie
J J
JJ
Xu
L W
LW
D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D006085:Graft Survival / Q000502:physiology*; D051550:I-kappa B Kinase / Q000235:genetics; D019194:Ischemic Preconditioning / Q000379:methods*; D016030:Kidney Transplantation / Q000473:pathology / Q000502:physiology*; D007813:Laparotomy; D008297:Male; D023421:Models, Animal; D016328:NF-kappa B / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016328:NF-kappa B; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine; D051550:I-kappa B Kinase