title: Role of anti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection from white spot syndrome virus infection.
abstract: The anti-lipopolysaccharide factor (ALF) from the black tiger shrimp, Penaeus monodon, has been shown previously to exhibit a broad spectrum of activity against various strains of bacteria and fungi. Herein, the recombinant ALFPm3 (rALFPm3) protein was examined for its role in the defence against white spot syndrome virus (WSSV) infection in haematopoietic (Hpt) cell cultures of the freshwater crayfish, Pacifastacus leniusculus, as well as in live P. monodon shrimps. Incubation of Hpt cell cultures with a mixture of WSSV and rALFPm3 resulted in a dose-dependent decrease in VP28 gene expression levels, compared with those incubated with WSSV alone, with an rALFPm3 IC50 value lower than 2.5 microM. However, pre-treatment of Hpt cells with 5 microM rALFPm3 showed no induced protection against subsequent WSSV infection, whereas the synthetic crayfish ALF peptide could protect cells at a higher concentration (10 microM). The in vivo role of ALFPm3 was examined by injection of P. monodon with WSSV pre-treated with rALFPm3 protein. The results clearly showed that rALFPm3 was able to reduce WSSV propagation and prolong the survival of shrimps.
authors.affiliation: Department of Comparative Physiology, Uppsala University, 75236 Uppsala, Sweden.|Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Sirinit
authors.initials: S
authors.lastname: Tharntada
authors.affiliation: Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Sirikwan
authors.initials: S
authors.lastname: Ponprateep
authors.affiliation: Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Kunlaya
authors.initials: K
authors.lastname: Somboonwiwat
authors.affiliation: Department of Comparative Physiology, Uppsala University, 75236 Uppsala, Sweden.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Comparative Physiology, Uppsala University, 75236 Uppsala, Sweden.
authors.forename: Irene
authors.initials: I
authors.lastname: Söderhäll
authors.affiliation: Department of Comparative Physiology, Uppsala University, 75236 Uppsala, Sweden.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Söderhäll
authors.affiliation: Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D003400:Astacoidea; D002478:Cells, Cultured; D007447:Invertebrate Hormones / Q000276:immunology* / Q000494:pharmacology; D033561:Penaeidae / Q000276:immunology* / Q000821:virology*; D016019:Survival Analysis; D014777:Virus Diseases / Q000517:prevention & control*; D046848:White spot syndrome virus 1 / Q000187:drug effects / Q000276:immunology*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D007447:Invertebrate Hormones; C034705:antilipopolysaccharide factor (Limulus)