title: Gel-pad microarrays templated by patterned porous silicon for dual-mode detection of proteins.
abstract: A proof-of-concept study demonstrated the feasibility of a novel gel-pad microarray on porous silicon chips, by initiation of an atom transfer radical propagation (ATRP) polymerisation of (polyethylene glycol) methacrylate (PEGMA) with surface Si-H species, stepwise chemical conversions of the gel membrane to an NTA-Ni2+/histidine-tagged protein system, and matrix-assisted laser desorption/ionisation mass spectroscopy (MALDI MS) and fluorescence detections.
authors.affiliation: State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Zeng-Tai
authors.initials: ZT
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shou-Jun
authors.initials: SJ
authors.lastname: Xiao
authors.forename: Zu-Hong
authors.initials: ZH
authors.lastname: Lu
authors.forename: Zhong-Ze
authors.initials: ZZ
authors.lastname: Gu
authors.forename: Lin
authors.initials: L
authors.lastname: Kang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Pei-Heng
authors.initials: PH
authors.lastname: Wu
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Tang
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Liu
mesh_terms: D005782:Gels / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties
chemical_list: D005782:Gels; D012825:Silicon