CAP37-derived antimicrobial peptides have in vitro antiviral activity against adenovirus and herpes simplex virus type 1.
OBJECTIVE
The antiviral activity of an established antibacterial CAP37 domain and its extracellular mechanism of action were investigated.


METHODS
CAP37-derived peptides modified to assess the importance of disulfide bonds were evaluated in cytotoxicity and antiviral assays (direct time kill, dose dependency, and TOTO-1) for adenovirus (Ad) and herpes simplex virus type 1 (HSV-1).


RESULTS
Variable virus, adenovirus serotype-dependent, and dose-dependent inhibition were demonstrated without cytotoxicity. For peptide A (CAP37(20-44)), TOTO-1 dye uptake was demonstrated for Ad5 and HSV-1.


CONCLUSIONS
Unlike the antibacterial activity of this CAP37 domain, its antiviral activity is not fully dependent upon disulfide bond formation. Viral inhibition appears to result, in part, from disruption of the envelope and/or capsid.
Gordon
Y Jerold
YJ
The Charles T. Campbell Lab Ophthalmic Microbiology Laboratory, UPMC Eye Center, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA. gordonjs@upmc.edu
Romanowski
Eric G
EG
Shanks
Robert M Q
RM
Yates
Kathleen A
KA
Hinsley
Heather
H
Pereira
H Anne
HA
D000260:Adenoviruses, Human / Q000187:drug effects* / Q000502:physiology; D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D001798:Blood Proteins / Q000737:chemistry / Q000494:pharmacology*; D002213:Capsid / Q000187:drug effects; D002352:Carrier Proteins / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents; D001798:Blood Proteins; D002352:Carrier Proteins; D010446:Peptide Fragments