title: Nuclear targeted nanoprobe for single living cell detection by surface-enhanced Raman scattering.
abstract: We present a novel nuclear targeting nanoprobe based on peptide functionalized gold nanoparticles and its surface-enhanced Raman scattering (SERS) in living cells. For the first time, we probe an original SERS signal from the living cell nucleus by using high-selectivity functionalized gold nanoparticles. The gold nanoparticles conjugated with SV-40 large T nuclear localization signal (NLS) peptide successfully enter the cell nucleus after the incubation with Hela cells and deliver the spatially localized chemical information of the nucleus, as well as the signature of chemicals that intruded subsequently. This new targeted nanoprobe is a nontoxic, biocompatible method for biological research, provided with multiple functions comprising subcellular targeting, intracellular imaging, and real-time SERS detection.
authors.affiliation: Wuhan University, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Le
authors.initials: L
authors.lastname: Su
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
authors.forename: Jinquan
authors.initials: J
authors.lastname: Tan
authors.forename: Jiming
authors.initials: J
authors.lastname: Hu
mesh_terms: D001692:Biological Transport; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007364:Intercalating Agents / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D007364:Intercalating Agents; D010455:Peptides; D006046:Gold; D004247:DNA