title: Direct analysis of valsartan or candesartan in human plasma and urines by on-line solid phase extraction coupled to electrospray tandem mass spectrometry.
abstract: AT1 receptor blockers are agents for the treatment of hypertension. Rapid and precise assay methods are needed to evaluate possible sub- or overdosage. A direct on-line solid phase extraction coupled to tandem mass spectrometry was developed and validated to determine valsartan (5-2000ng/mL) or candesartan (1-200ng/mL) in human plasma and urines. Both intra- and inter-assay accuracy and precision were in the range 89.2-111% and 0.9-16.9% RSD. Total run time was 4.5min. This on-line clean-up-MS method was found to be robust for the analysis of a high number of samples with unvarying performance.
authors.affiliation: CEA, iBiTecS, Service de Pharmacologie et d'Immunoanalyse, Gif-sur-Yvette F-91191, France.
authors.forename: Mikaël
authors.initials: M
authors.lastname: Levi
authors.forename: Grégoire
authors.initials: G
authors.lastname: Wuerzner
authors.forename: Eric
authors.initials: E
authors.lastname: Ezan
authors.forename: Alain
authors.initials: A
authors.lastname: Pruvost
mesh_terms: D001562:Benzimidazoles / Q000097:blood* / Q000652:urine*; D001713:Biphenyl Compounds; D006801:Humans; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013777:Tetrazoles / Q000097:blood* / Q000652:urine*; D014633:Valine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D000068756:Valsartan
chemical_list: D001562:Benzimidazoles; D001713:Biphenyl Compounds; D013777:Tetrazoles; D000068756:Valsartan; D014633:Valine; C081643:candesartan