Cloning and characterization of cDNAs encoding ent-copalyl diphosphate synthases in wheat: insight into the evolution of rice phytoalexin biosynthetic genes.
In vitro assays using recombinant enzymes enabled three cDNAs encoding ent-copalyl diphosphate synthases to be identified in wheat (Triticum aestivum): TaCPS1, TaCPS2, and TaCPS3. The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice.
Toyomasu
Tomonobu
T
Department of Bioresource Engineering, Yamagata University, Tsuruoka, Yamagata, Japan. toyomasu@tds1.tr.yamagata-u.ac.jp
Kagahara
Takuma
T
Hirose
Yuko
Y
Usui
Masami
M
Abe
Shiho
S
Okada
Kazunori
K
Koga
Jinichiro
J
Mitsuhashi
Wataru
W
Yamane
Hisakazu
H
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D019143:Evolution, Molecular; D017343:Genes, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics; D000094162:Phytoalexins
D018076:DNA, Complementary; D010940:Plant Proteins; D012717:Sesquiterpenes; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C090963:ent-kaurene synthetase A; D000094162:Phytoalexins