Synthesis of puromycin derivatives with backbone-elongated substrates and associated translation inhibitory activities.
We have synthesized a series of 5'-phosphorylated and 5'-cytidylyl-(3'-5')-cytidylyl-(3'-5')-puromycin derivatives that have backbone-elongated substrates. All the synthesized puromycin derivatives showed good solubility in water and were applied to translation inhibitory assay in a reconstituted Escherichia coli translation system.
Mizusawa
Keigo
K
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Abe
Kenji
K
Sando
Shinsuke
S
Aoyama
Yasuhiro
Y
D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000235:genetics; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects*; D011691:Puromycin / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011691:Puromycin