title: Structural identification and cytotoxic activity of a polysaccharide from the fruits of Lagenaria siceraria (Lau).
abstract: A water-soluble polysaccharide, isolated from fruiting bodies of Lagenaria siceraria, is composed of methyl-alpha-d-galacturonate, 3-O-acetyl methyl-alpha-d-galacturonate, and beta-d-galactose in a ratio of nearly 1:1:1. Compositional analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, 2D-COSY, TOCSY, NOESY, HMQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide: [carbohydrate structure: see text] This polysaccharide showed cytotoxic activity in vitro against human breast adenocarcinoma cell line (MCF-7).
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
authors.forename: Kaushik
authors.initials: K
authors.lastname: Ghosh
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Chandra
authors.forename: Arnab K
authors.initials: AK
authors.lastname: Ojha
authors.forename: Siddik
authors.initials: S
authors.lastname: Sarkar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019662:Cucurbitaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides