Immunosuppressive function of bone marrow mesenchymal stem cells on acute rejection of liver allografts in rats.
Bone marrow mesenchymal stem cells (MSCs) demonstrate functions of immunologic regulation. However, little is known about the role of interferon-gamma (IFN-gamma) on MSCs and whether MSCs alone can prevent allograft rejection. We purified MSCs, which were or were not treated with IFN-gamma, to act as regulatory cells in mixed lymphocyte reactions. We measured their expression of PDL-1, MHC-I, MHC-II, CD40, CD54, and CD86. The MSCs stained with carboxyfluorescein diacetate-succinimidyl ester were used to detect homing in vivo. The MSCs were injected into an orthotopic liver transplantation model. The result suggested that IFN-gamma enhances expression of PDL-1, MHC-I, MHC-II, and CD54 and boosts immunosuppressive ability in vivo. The MSCs demonstrated homing to the liver, alleviating acute immunologic rejection of an hepatic graft in rats. We conclude that IFN-gamma may enhance the immunosuppressive function of MSCs to protect liver allografts in rats from acute immunologic rejection.
Hong
Z-F
ZF
Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Xiamen, China.
Huang
X-J
XJ
Yin
Z-Y
ZY
Zhao
W-X
WX
Wang
X-M
XM
D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006084:Graft Rejection / Q000276:immunology* / Q000517:prevention & control; D007165:Immunosuppression Therapy; D017493:Leukocyte Common Antigens / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D011914:Rats, Inbred BN; D051876:Sex-Determining Region Y Protein / Q000235:genetics; D018800:Thy-1 Antigens / Q000276:immunology; D018183:Transplantation Chimera; D014998:Y Chromosome
D000939:Epitopes; D051876:Sex-Determining Region Y Protein; C497747:Sry protein, rat; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens