Quantitative differential proteome analysis in an animal model for human melanoma.
In fish of the genus Xiphophorus, different grades of pigment cell lesions from nevi to melanoma can be gained by simple crossbreeding. With this model, one can easily access tissues of different malignancies from animals with highly identical genetic background. To find protein expression differences between healthy, benign and malignant tissues, we performed 2D PAGE and DIGE and found among regulated proteins antioxidant proteins that were overexpressed with increasing malignancy.
Lokaj
Katrin
K
Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Wurzburg, Versbacher Str. 9, 97078 Wurzburg, Germany.
Meierjohann
Svenja
S
Schütz
Claudia
C
Teutschbein
Janka
J
Schartl
Manfred
M
Sickmann
Albert
A
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D003532:Cyprinodontiformes / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008546:Melanoma, Experimental / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*
D014408:Biomarkers, Tumor; D020543:Proteome