title: Synovial sarcoma: diagnosis on fine-needle aspiration by morphology and molecular analysis.
abstract: BACKGROUND
  Synovial sarcoma (SS) is characterized by the t(X; 18) (p11.2; q11.2) translocation resulting in the SYT-SSX fusion transcript, detectable by reverse transcriptase polymerase chain reaction (RT-PCR). Fine-needle aspiration (FNA) cytology diagnosis of SS is challenging. We evaluated the applicability of RT-PCR on FNAs and to perform a detailed cytomorphological analysis in unequivocal cases of SS.
  
  
  METHODS
  A prospective and retrospective analysis was performed over 4 years (2003-2007). Prospectively, FNAs positive for the SYT-SSX fusion transcript by RT-PCR (n = 6) and, retrospectively, cases proven on histopathology and immunohistochemistry (ICC; positivity for vimentin and epithelial membrane antigen [EMA]/cytokeratin) as SS (n = 10) were included in the study. A detailed cytomorphological analysis was carried out.
  
  
  RESULTS
  There were 9 biphasic and 7 monophasic tumors. The aspirates from both biphasic and monophasic tumors were richly cellular in all cases with micro tissue fragments. Pericapillary arrangement of tumor cells was present in most cases. Attempted gland formation was seen in 7 of 9 biphasic tumors. The individual tumor cells were round, ovoid, or spindle shaped. Pleomorphism was mild; monophasic tumors displayed lesser pleomorphism as compared with the biphasic ones. Nuclear chromatin was bland in all cases except 1 and nucleolar prominence was seen in just 3 biphasic tumors. Mast cells were seen in 3 biphasic and 2 monophasic tumors. Scanty to moderate extracellular matrix material was seen in 5 cases.
  
  
  CONCLUSIONS
  FNA cytology of SS shows a spectrum of cytomorphological features; the diagnosis is confirmed by RT-PCR on the aspirated material for the SYT-SSX fusion transcript.
authors.affiliation: Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. drsradhika@gmail.com
authors.forename: Radhika
authors.initials: R
authors.lastname: Srinivasan
authors.forename: Upasana
authors.initials: U
authors.lastname: Gautam
authors.forename: Ruchi
authors.initials: R
authors.lastname: Gupta
authors.forename: Arvind
authors.initials: A
authors.lastname: Rajwanshi
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Vasistha
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D044963:Biopsy, Fine-Needle; D003581:Cytodiagnosis / Q000379:methods; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000032:analysis; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D011446:Prospective Studies; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013584:Sarcoma, Synovial / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D017422:Sequence Analysis, DNA; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000032:analysis; D055815:Young Adult
chemical_list: D018396:Mucin-1; D015514:Oncogene Proteins, Fusion; C116512:SYT-SSX fusion protein; D014746:Vimentin; D007633:Keratins