Stability of analytes related to clinical chemistry and bone metabolism in blood specimens after delayed processing.
OBJECTIVE
We investigated the stability of 36 analytes related to clinical chemistry in a controlled storage study.


METHODS
Blood was collected from 11 subjects and was maintained for 45 min, 2.5 h, 5 h, or 24 h after phlebotomy before centrifugation.


RESULTS
Statistically significant changes were observed only for parathyroid hormone, osteocalcin, zinc, pyridoxal 5'-phosphate, and homocysteine.


CONCLUSIONS
These studies indicate that many analytes in clinical chemistry are stable for 24 h before centrifugation.
Zwart
Sara R
SR
Universities Space Research Association, Houston, TX, USA. sara.zwart-1@nasa.gov
Wolf
Megan
M
Rogers
Ann
A
Rodgers
Shanna
S
Gillman
Patti L
PL
Hitchcox
Kristen
K
Ericson
Karen L
KL
Smith
Scott M
SM
D001842:Bone and Bones / Q000378:metabolism*; D002498:Centrifugation; D002624:Chemistry, Clinical / Q000379:methods*; D006801:Humans; D015675:Osteocalcin / Q000097:blood; D010281:Parathyroid Hormone / Q000097:blood; D018962:Phlebotomy; D011732:Pyridoxal Phosphate / Q000097:blood; D013048:Specimen Handling; D013997:Time Factors
C470125:PTH protein, human; D010281:Parathyroid Hormone; D015675:Osteocalcin; D011732:Pyridoxal Phosphate