Biosensor immunoassay for traces of hazelnut protein in olive oil.
The fraudulent addition of hazelnut oil to more expensive olive oil not only causes economical loss but may also result in problems for allergic individuals as they may inadvertently be exposed to potentially allergenic hazelnut proteins. To improve consumer safety, a rapid and sensitive direct biosensor immunoassay, based on a highly specific monoclonal antibody, was developed to detect the presence of hazelnut proteins in olive oils. The sample preparation was easy (extraction with buffer); the assay time was fast (4.5 min only) and the limit of detection was low (0.08 microg/g of hazelnut proteins in olive oil). Recoveries obtained with an olive oil mixed with different amounts of a hazelnut protein containing hazelnut oil varied between 93% and 109%.
Bremer
Maria G E G
MG
RIKILT-Institute of Food Safety, Wageningen UR, P.O. Box 230, 6700 AE Wageningen, The Netherlands. monique.bremer@wur.nl
Smits
Nathalie G E
NG
Haasnoot
Willem
W
D015374:Biosensing Techniques / Q000379:methods*; D003257:Consumer Product Safety; D031211:Corylus / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis*
D000069463:Olive Oil; D010938:Plant Oils; D010940:Plant Proteins