title: Open tubular layer of S-ofloxacin imprinted polymer fabricated in silica capillary for chiral CEC separation.
abstract: An open tubular molecule imprinted polymer (OT-MIP) capillary column has been prepared for chiral separation of ofloxacin enantiomers in CEC. The S-ofloxacin imprinted OT column was fabricated by thermally initiated non-covalent polymerization procedure inside a pretreated and silanized fused silica capillary. The template molecule was incorporated with methacrylic acid (MAA), ethylene glycol dimethacrylate (EDMA) and 4-styrenesulfonic acid (4-SSA) and dissolved in a porogen mixture of ACN/2-propanol (9:1). The separation efficiency of the 4-SSA MIP column was found quite better than that of the MIP column without 4-SSA. It has been demonstrated that our OT-MIP column can separate ofloxacin enantiomers with excellent chiral separation efficiency after tuning the various chromatographic conditions. The optimized chromatographic eluent was 85:15, v/v%, ACN/60 mM sodium acetate at pH 7. The separation efficiency and selectivity of chiral separation of this study were far better than those obtained by previous methods for chiral separation of R- and S-ofloxacin.
authors.affiliation: Department of Chemistry, Nano Fine Center, and Institute of Basic Research, Inha University, Incheon, South Korea.
authors.forename: Shabi Abbas
authors.initials: SA
authors.lastname: Zaidi
authors.forename: Kyoung Moon
authors.initials: KM
authors.lastname: Han
authors.forename: Sung Soon
authors.initials: SS
authors.lastname: Kim
authors.forename: Dong Guk
authors.initials: DG
authors.lastname: Hwang
authors.forename: Won Jo
authors.initials: WJ
authors.lastname: Cheong
mesh_terms: D002021:Buffers; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D015394:Molecular Structure; D015242:Ofloxacin / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013343:Styrenes; D013451:Sulfonic Acids
chemical_list: D002021:Buffers; D011108:Polymers; D013343:Styrenes; D013451:Sulfonic Acids; D012822:Silicon Dioxide; D015242:Ofloxacin