Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.
Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.
Hurst
S F
SF
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Kidd
S E
SE
Morrissey
C O
CO
Snelders
E
E
Melchers
W J G
WJ
Castelli
M V
MV
Mellado
E
E
Simmon
K
K
Petti
C A
CA
Richardson
S
S
Zhang
S
S
Romanelli
A M
AM
Wickes
B L
BL
de Valk
H A
HA
Klaassen
C H W
CH
Balajee
S Arunmozhi
SA
D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D005838:Genotype; D016533:Mycological Typing Techniques / Q000379:methods* / Q000592:standards*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000592:standards*
D004271:DNA, Fungal