title: Design of a human synthetic combinatorial library of single-chain antibodies.
abstract: Antibody libraries came into existence 15 years ago when the accumulating sequence data of immunoglobulin genes and the advent of the PCR technology made it possible to clone antibody gene repertoires. Phage display (most common) and additional display and screening technologies were applied to pan out desired binding specificities from antibody libraries. "Synthetic" or "semi-synthetic" libraries are from naïve, non-immunized source and considered to be a source for many different targets, including self-antigens. We describe here how to construct a large human synthetic single-chain Fv (scFv) antibody library displayed on phages, where in vivo-formed complementarity-determining regions (CDRs) are shuffled combinatorially onto germline-derived human variable-region frameworks.
authors.affiliation: Tel-Aviv University, Ramat Aviv, Israel.
authors.forename: Limor
authors.initials: L
authors.lastname: Nahary
authors.forename: Itai
authors.initials: I
authors.lastname: Benhar
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000915:Antibody Affinity; D000941:Antigens / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D022801:Complementarity Determining Regions / Q000276:immunology; D018274:Electroporation; D004926:Escherichia coli / Q000166:cytology; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D055767:Immobilized Proteins / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D019151:Peptide Library; D016133:Polymerase Chain Reaction
chemical_list: D000906:Antibodies; D000941:Antigens; D022801:Complementarity Determining Regions; D055767:Immobilized Proteins; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D019151:Peptide Library