title: Nanostructured FeS as a mimic peroxidase for biocatalysis and biosensing.
abstract: Artificial enzyme mimics have attracted considerable interest due to easy denaturation and leakage of enzymes during their storage and immobilization procedure. Herein we describe the design of a novel mimic peroxidase, a nanostructure of sheet-like FeS prepared by a simple micelle-assisted synthetic method. Such a nanostructure has a large specific surface area and high peroxidase-like activity, and was thus further used as a mimic enzyme for the development of biocatalysts and amperometric biosensors. The sheet-like FeS nanostructure showed typical Michaelis-Menten kinetics and good affinity to both H(2)O(2) and 3,3',5,5'-tetramethyl benzidine. At pH 7.0 the constructed amperometric sensor showed a linear range for the detection of H(2)O(2) from 0.5 to 150 microM with a correlation coefficient of 0.9998 without any electron transfer mediator. The H(2)O(2) sensor based on the sheet-like FeS showed more sensitive response than those based on spherical FeS nanostructure, and resulted in a better stability than horseradish peroxidase when they were exposed to solutions with different pH values and temperatures. These excellent properties made the sheet-like nanostructured FeS powerful tools for a wide range of potential applications as an "artificial peroxidase" as biosensors and biotechnology.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210097, PR China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Dai
authors.forename: Shaohua
authors.initials: S
authors.lastname: Liu
authors.forename: Jianchun
authors.initials: J
authors.lastname: Bao
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D018716:Molecular Mimicry; D049329:Nanostructures / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005296:Ferrous Compounds; D009195:Peroxidase; C022597:ferrous sulfide