Simplified procedures for the isolation of HF3, bothropasin, disintegrin-like/cysteine-rich protein and a novel P-I metalloproteinase from Bothrops jararaca venom.
HF3 and bothropasin are P-III hemorrhagic snake venom metalloproteinases (SVMPs) of Bothrops jararaca. The DC protein is composed of the disintegrin-like/cysteine-rich domains derived from the autolysis of P-III SVMPs. Here we describe simplified procedures for the isolation of HF3, bothropasin, the DC protein, and BJ-PI, a novel P-I SVMP. The isolated proteins were identified by mass spectrometry. BJ-PI is a potent caseinolytic enzyme devoid of hemorrhagic activity. HF3, bothropasin and BJ-PI show distinct fibrinogenolytic activities.
Oliveira
Ana K
AK
Laboratório Especial de Toxinologia Aplicada-CAT-CEPID, Instituto Butantan, São Paulo, Brazil.
Paes Leme
Adriana F
AF
Assakura
Marina T
MT
Menezes
Milene C
MC
Zelanis
André
A
Tashima
Alexandre K
AK
Lopes-Ferreira
Mônica
M
Lima
Carla
C
Camargo
Antonio C M
AC
Fox
Jay W
JW
Serrano
Solange M T
SM
D000595:Amino Acid Sequence; D000818:Animals; D017837:Bothrops / Q000502:physiology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D006470:Hemorrhage / Q000139:chemically induced / Q000473:pathology; D008666:Metalloendopeptidases / Q000302:isolation & purification* / Q000633:toxicity; D045726:Metalloproteases / Q000302:isolation & purification* / Q000633:toxicity; D051379:Mice; D008969:Molecular Sequence Data; D014357:Trypsin / Q000737:chemistry; D014757:Viper Venoms / Q000201:enzymology*
D003435:Crotalid Venoms; D014757:Viper Venoms; D045726:Metalloproteases; D014357:Trypsin; C543527:HF3 protein, Bothrops jararaca; D008666:Metalloendopeptidases; C472493:bothropasin, Bothrops jararaca