Flow-injection chemiluminescence method for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride.
A sensitive and simple flow-injection chemiluminescence (FI-CL) method, which was based on the CL intensity generated from the redoxreaction of potassium permanganate (KMnO4)-formaldehyde in vitriol (H2SO4) medium, has been developed, validated and applied for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride. Besides oxidants and sensitizers, the effect of the concentration of H(2)SO(4), KMnO4 and formaldehyde was investigated. Under the optimum conditions, the linear range was 1.0 x 10(-2)-7.0 mg/L for naphazoline hydrochloride and 5.0 x 10(-2)-10.0 mg/L for oxymetazoline hydrochloride. During seven repeated inter-day and intra-day precision tests of 0.1, 1.0 and 10.0 mg/L samples, the relative standard deviations all corresponded to reference values. The detection limit was 8.69 x 10(-3) mg/L for naphazoline hydrochloride and 3.47 x 10(-2) mg/L for oxymetazoline hydrochloride (signal-to-noise ratio < or = 3). This method has been successfully implemented for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride in pharmaceuticals.
Wang
Nan-Nan
NN
Department of Medicament, The Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, People's Republic of China. nami_wan@163.com
Shao
Yan-Qing
YQ
Tang
Yu-Hai
YH
Yin
He-Ping
HP
Wu
Xiao-Zhong
XZ
D002138:Calibration; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005557:Formaldehyde / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D009278:Naphazoline / Q000032:analysis*; D010109:Oxymetazoline / Q000032:analysis*; D011196:Potassium Permanganate / Q000737:chemistry; D012680:Sensitivity and Specificity
D011196:Potassium Permanganate; D005557:Formaldehyde; D010109:Oxymetazoline; D009278:Naphazoline