The quest for a message: budding yeast, a model organism to study the control of pre-mRNA splicing.
Removal of introns during pre-mRNA splicing is a critical process in gene expression, and understanding its control at both single-gene and genomic levels is one of the great challenges in Biology. Splicing takes place in a dynamic, large ribonucleoprotein complex known as the spliceosome. Combining Genetics and Biochemistry, Saccharomyces cerevisiae provides insights into its mechanisms, including its regulation by RNA-protein interactions. Recent genome-wide analyses indicate that regulated splicing is broad and biologically relevant even in organisms with a relatively simple intronic structure, such as yeast. Furthermore, the possibility of coordination in splicing regulation at genomic level is becoming clear in this model organism. This should provide a valuable system to approach the complex problem of the role of regulated splicing in genomic expression.
Meyer
Markus
M
Gene Regulation Program, Centre de Regulació Genòmica, Dr Aiguader 88, Barcelona, Spain.
Vilardell
Josep
J
D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D015966:Gene Expression Regulation, Fungal; D016678:Genome; D016681:Genome, Fungal; D007438:Introns; D008540:Meiosis; D009687:Nuclear Proteins / Q000378:metabolism; D025941:Protein Interaction Mapping; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; C092601:MER1 protein, S cerevisiae; D009687:Nuclear Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae