title: Analysis of the complete genome sequence of epidemic keratoconjunctivitis-related human adenovirus type 8, 19, 37 and a novel serotype.
abstract: We determined the complete genome sequence of epidemic keratoconjunctivitis (EKC)-related human adenoviruses (HAdVs). We analysed a total of 12 HAdV strains; three prototype strains and two HAdV-8, three HAdV-19 and three HAdV-37 clinical isolates from EKC patients in Japan, and one novel serotype of HAdV. Genome organization of these serotypes was identical to those of the recently determined HAdV-19 and HAdV-37. The identities of the whole genome were over 99 % among strains from the same serotype, except for HAdV-19p, which is not associated with conjunctivitis, resulting in the formation of a distinct cluster in the phylogenetic analysis. The penton, loop 1 and loop 2 of hexon, early region 3 (E3) and fiber were hypervariable regions between serotypes. Results suggest that the HAdV-19 clinical strain is a recombinant of HAdV-19p-like and HAdV-37-like strains, and that the acquisition of the penton, E3 or fiber may be related to ocular tropism.
authors.affiliation: Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kaneko
authors.affiliation: Department of Ophthalmology, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Iida
authors.affiliation: Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohguchi
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Ariga
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Tagawa
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
authors.affiliation: Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzutani
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny
chemical_list: D004279:DNA, Viral