[Application of created restriction site PCR-RFLP to identify alcohol dehydrogenase 2 gene polymorphism].
OBJECTIVE
To develop a appropriate assay for identifying single nucleotide polymorphism (SNP) of alcohol dehydrogenase 2 (ADH2) gene.


METHODS
According to base substitution situation of one single base mutational site, we designed the present study primers. One of the primers was designed on the basis of neighbourhood sequence of the mutational site, that is, we made one mismatch base to let product a new enzyme site between the 3' end of the primer and the single base mutation type after the PCR amplification. Then PCR-RFLP was adopted to identify the SNP in ADH2 gene.


RESULTS
One primer pair can get target products containing ADH2 SNP site by PCR, restriction enzymes Bsh1236I were adopted to identify the SNP site. The expected results were reached.


CONCLUSIONS
It suggested that the method of detecting the SNP of ADH2 based on CRS-PCR-RFLP theory is facilitated, economic, and rapid.
Jiao
Jie
J
Department of Occupational Health, School of Public Health, Fudan University, Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China. jiaojie66@126.com
Wang
Wei
W
Liu
Jing
J
Xia
Zhaolin
Z
D000328:Adult; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D015183:Restriction Mapping / Q000379:methods; D055815:Young Adult
C489332:ADH1B protein, human; D000426:Alcohol Dehydrogenase