title: Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor.
abstract: A layer-by-layer route to prepare nanoporous Au film materials on transparent ITO substrates is reported by alternatively assembling Au and Ag nanoparticles through 1,5-pentanedithiol as a cross-linker, followed by that Ag nanoparticles are dissolved at room temperature in HAuCl4 solution. Electron transfer of cytochrome c (cyt. c) - an excellent model for investigation of biomolecules, is greatly facilitated at the nanoporous Au film with electron transfer rate constant (ks) of 3.9s(-1). Meanwhile, cyt. c adsorped onto the nanoporous Au film still maintain its enzymatic activity toward H2O2. On the basis of these experimental results, the cyt. c-nanoporous Au film is exploited to an amperometric biosensor for H2O2 with high selectivity, broad linear range, low detection limit, and long stability.
authors.affiliation: Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.forename: Haiqing
authors.initials: H
authors.lastname: Liu
authors.forename: Yongping
authors.initials: Y
authors.lastname: Luo
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D045304:Cytochromes c / Q000502:physiology; D004579:Electron Transport; D006046:Gold; D006861:Hydrogen Peroxide / Q000032:analysis*; D008422:Materials Testing; D053758:Nanoparticles; D017971:Tin Compounds
chemical_list: D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold; D045304:Cytochromes c; D006861:Hydrogen Peroxide