[Regulation of integrin alpha2, alpha5, beta1 mRNA expression on osteoblasts membrane by bFGF].
OBJECTIVE
To observe the regulation of bFGF on mRNA expression of integrin subunits alpha2, alpha5, beta1, in osteoblasts.


METHODS
Osteoblasts were pre-cultured for 24 h with F12 medium containing bFGF with different concentration and planted on the sand blasting titanium disks. Three days after planting, osteoblasts were harvested. The mRNA expression of integrin subunit alpha2, alpha5, beta1, were examined by immunofluorescent quantitative real-time polymerase chain reaction (RT-PCR).


RESULTS
The mRNA expression of integrin beta1 is most abundant and the mRNA expressions of integrin alpha2, as are relatively lower than integrin beta1. In the study, the mRNA expression levels of integrin alpha2, alpha5 and beta1 were up-regulated after bFGF stimulation. However, the effective concentration range of bFGF to the three subunits was different.


CONCLUSIONS
bFGF could up-regulate the mRNA expression of integrin subunits in osteoblasts.
Xia
Lu
L
Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Wang
Pei-zhi
PZ
Liang
Xing
X
Xu
Ling
L
Wang
Guo-ping
GP
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism*; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D039421:Integrin alpha2; D039482:Integrin alpha5; D019012:Integrin beta1; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2