[Genomic comparisons of multiresistant Pseudomonas aeruginosa isolates obtained from surgical intensive care unit].
OBJECTIVE
To investigate the genetic differences and their relativity with multi-drug resistance of Pseudomonas aeruginosa isolated.


METHODS
Forty-nine Pseudomonas aeruginosa isolates were characterized by antimicrobial susceptibility and four-enzyme (I-CeuI, SpeI, SwaI, PacI) pulsed-field gel electrophoresis (PFGE).


RESULTS
85.7% of the P.aeruginosa isolates were MDR strains. Strains with PFGE pattern A were all susceptible to amikacin and cefepime, but were resistant to levofloxacin and meropenem. Strains with PFGE Patterns H and P had resistance to 6 - 8 different kinds of antibiotics. Strains with PFGE Patterns I and J were susceptible to all antibiotics tested in this study. Strains with other PFGE Patterns had intermediate resistance. PFGE pattern A was the dominant pattern, which accounted for 61.2% of all P.aeruginosa strains, 100% (2/2) in 2001, 65% (13/20) in 2002, 44.4% (8/18) in 2003 and 77.8% (7/9) in 2004.


CONCLUSIONS
Four-enzyme combined PFGE analysis is highly discriminatory for the subtyping of MDR P.aeruginosa isolates.
Wang
Yu-Ming
YM
Department of General Surgery, Peking University First Hospital, Beijing 100034, China.
Liu
Yu-Cun
YC
Wan
Yuan-Lian
YL
Liu
Shu-Lin
SL
Liu
Gui-Rong
GR
Wang
Xin
X
Wang
Qiu-Shi
QS
Cao
Xiao-Li
XL
D015373:Bacterial Typing Techniques; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects* / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D007362:Intensive Care Units; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification