High-performance liquid chromatographic determination of ustiloxin A in forage rice silage.
We describe a high-performance liquid chromatographic method for determining ustiloxin A, a mycotoxin produced by Ustilaginoidea virens, in forage rice silage. Lyophilized silage samples were ground and extracted with water. The extracts were purified by solid-phase extraction and subjected to high-performance liquid chromatography using an octadecylsilane-bonded column. Separated ustiloxin A was detected with ultraviolet (UV) absorption at 254 nm. The limit of quantitation for ustiloxin A in silage found to be 2.5 mg/kg. The present method can be used for routine monitoring of the contamination of ustiloxin A in forage rice silage.
Miyazaki
Shigeru
S
Safety Research Team, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, Japan. s.miyazaki@affrc.go.jp
Matsumoto
Yuichi
Y
Uchihara
Tsutomu
T
Morimoto
Kazuhide
K
D001203:Ascomycota; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005612:Freeze Drying; D012275:Oryza / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000032:analysis*; D012820:Silage / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010456:Peptides, Cyclic; C087237:ustiloxin A