Comparisons among sensitivities of different tumor cells to focused ultrasound in vitro.
This study is to test the sensitivities of different tumor cells to ultrasound irradiation at the frequency of 2.2 MHz for 60 s duration, and investigate the potential mechanism underlying different sensitivities. Three murine tumor models with distinct aggressiveness (S180, H-22 and EAC) were exposed to ultrasound to evaluate their sonodynamic efficiencies, and several biological parameters such as cell membrane permeability, lipid peroxidation (LPO), ultra-structure observation, intracellular reactive oxygen species (ROS) and mitochondria membrane potential (MMP) were analyzed after exposures. The results showed that cellular responses of different cells were distinct, of interest to note, the aggressive S180 cells were much more sensitive than others, whereas EAC cells were relatively more resistant to ultrasound irradiation. The direct comparisons among different types of cells indicate that the sono-sensitization seems to depend on the physiological and chemical properties of tumor cells. Perhaps sections of cell membrane became destabilized following the initial radical attack and LPO reaction, which caused S180 cells more susceptible to mechanical stresses during sonolysis. This study provides important implications for cancer therapy.
Wang
XiaoBing
X
College of Life Sciences, Shaanxi Normal University, Shaanxi, Xian 710062, China.
Liu
QuanHong
Q
Wang
Pan
P
Wang
ZheZhi
Z
Tong
WanYan
W
Zhu
Bo
B
Wang
Yuan
Y
Li
ChenDi
C
D000818:Animals; D045744:Cell Line, Tumor / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D005434:Flow Cytometry; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000378:metabolism; D009369:Neoplasms / Q000628:therapy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012680:Sensitivity and Specificity; D014464:Ultrasonic Therapy / Q000379:methods; D014465:Ultrasonics
D017382:Reactive Oxygen Species