title: Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats.
abstract: OBJECTIVE
  Hydrogen has been reported to selectively reduce the hydroxyl radical, the most cytotoxic of reactive oxygen species. In this study we investigated the effects of hydrogen-rich saline on the prevention of lung injury induced by intestinal ischemia/reperfusion (I/R) in rats.
  
  
  METHODS
  Male Sprague-Dawley rats (n=30, 200-220g) were divided randomly into three experimental groups: sham operated, intestinal I/R plus saline treatment (5ml/kg, i.v.), and intestinal I/R plus hydrogen-rich saline treatment (5ml/kg, i.v.) groups. Intestinal I/R was produced by 90min of intestinal ischemia followed by a 4h of reperfusion.
  
  
  RESULTS
  Hydrogen-rich saline treatment decreased the neutrophil infiltration, the lipid membrane peroxidation, NF-kappaB activation and the pro-inflammatory cytokine interleukin IL-1beta and TNF-alpha in the lung tissues compared with those in saline-treated rat.
  
  
  CONCLUSIONS
  Hydrogen-rich saline attenuates lung injury induced by intestinal I/R.
authors.affiliation: Department of Surgical Intensive Care Unit, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, PR China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Mao
authors.forename: Xing-Feng
authors.initials: XF
authors.lastname: Zheng
authors.forename: Jian-Mei
authors.initials: JM
authors.lastname: Cai
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: You
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Deng
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
authors.forename: Lai
authors.initials: L
authors.lastname: Jiang
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Sun
mesh_terms: D000818:Animals; D002462:Cell Membrane; D006859:Hydrogen / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000378:metabolism; D007422:Intestines / Q000098:blood supply; D015227:Lipid Peroxidation; D055370:Lung Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications; D012965:Sodium Chloride / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D006859:Hydrogen