Immunosuppressive effects of new cyclolanostane triterpene diglycosides from the aerial part of Cimicifuga foetida.
Two new cyclolanostane diglycosides, cimifoetiside A (1) and cimifoetiside B (2), were isolated from an 80% ethanolic extract of the aerial part of Cimicifuga foetida L. (Ranuculaceae). Using spectral data and chemical analysis, the structures of 1 and 2 were identified as (23R, 24S) cimicigenol 3-O-beta-D-glucopyranosyl-(1''-->3')-beta-D-xylopyranoside and (23R, 24S) cimicigenol 3-O-beta-D-glucopyranosyl-(1''-->2')-beta-D-xylopyranoside, respectively. The in vitro immunosuppressive effects of the two new compounds 1 and 2, as well as four other known cyclolanostane saponins 3-6 on T cells were evaluated. All the agents tested effectively inhibited the proliferation of murine splenocytes induced by Concanavalin A (ConA), with IC(50) values ranging from 12.7 nM to 33.3 nM.
Pan
Rui-Le
RL
Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Chen
Di-Hua
DH
Si
Jian-Yong
JY
Zhao
Xiao-Hong
XH
Li
Zhan
Z
Cao
Li
L
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D031885:Cimicifuga / Q000737:chemistry*; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D006027:Glycosides; D007166:Immunosuppressive Agents; D014315:Triterpenes; C540201:cimifoetiside A; C540202:cimifoetiside B; D003208:Concanavalin A