HPLC determination of irbesartan in human plasma: its application to pharmacokinetic studies.
A simple and rapid HPLC method using fluorescence detection was developed for determination of irbesartan in human plasma. Sample preparation was accomplished through a simple deproteinization procedure with 0.4 mL of acetonitrile containing 800 ng/mL of losartan (internal standard), and to a 0.1 mL plasma sample. Chromatographic separation was performed on a Zorbax Xclipse XDB C18 column (150 x 4.6 mm, i.d., 5 microm) at 40 degrees C. An isocratic mobile phase, acetonitrile:0.1% formic acid (37:63, v/v), was run at a flow-rate of 1.0 mL/min, and the column eluent was monitored using a fluorescence detector set at excitation and emission wavelengths of 250 and 370 nm, respectively. The retention times of irbesartan and losartan were 4.4 and 5.9 min, respectively. This assay was linear over a concentration range of 10-5000 ng/mL with a lower limit of quantification of 10 ng/mL. The coefficient of variation for this assay precision was less than 8.48%, and the accuracy exceeded 94.4%. The mean relative recoveries of irbesartan and losartan were 98.4 and 99.1%, respectively. This method was successfully applied for pharmacokinetic study after oral administration of irbesartan (300 mg) to 23 Korean healthy male volunteers.
Bae
Soo Kyung
SK
Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, Korea. baesk@busanpaik.ac.kr
Kim
Min-Jung
MJ
Shim
Eon-Jeong
EJ
Cho
Doo-Yeoun
DY
Shon
Ji-Hong
JH
Liu
Kwang-Hyeon
KH
Kim
Eun-Young
EY
Shin
Jae-Gook
JG
D000097:Acetonitriles / Q000737:chemistry; D000328:Adult; D047228:Angiotensin II Type 1 Receptor Blockers / Q000097:blood* / Q000493:pharmacokinetics*; D001713:Biphenyl Compounds / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D006801:Humans; D000077405:Irbesartan; D019808:Losartan / Q000097:blood; D008297:Male; D015203:Reproducibility of Results; D018401:Sample Size; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000191:economics / Q000379:methods; D013777:Tetrazoles / Q000097:blood* / Q000493:pharmacokinetics*; D013997:Time Factors; D055815:Young Adult
D000097:Acetonitriles; D047228:Angiotensin II Type 1 Receptor Blockers; D001713:Biphenyl Compounds; D013777:Tetrazoles; D000077405:Irbesartan; D019808:Losartan; C032159:acetonitrile