Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice.
K88 (F4) fimbrial adhesin, FaeG, was expressed extracellularly in Lactococcus lactis using a nisin-controlled gene expression system. The antibody response and protective efficacy of the recombinant bacteria (L. lactis [spNZ8048-faeG]) against live enterotoxigenic E. coli (ETEC) C(83549) challenge were evaluated in ICR mice. Mice vaccinated with L. lactis [spNZ8048-faeG] had a significantly increased antigen-specific IgG level in the serum and decreased mortality rate (P < 0.05) compared with the control. This indicates that oral immunization of L. lactis [spNZ8048-faeG] can induce an immune-response protection upon challenge with live ETEC in ICR mice.
Hu
Chun Xia
CX
Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, People's Republic of China.
Xu
Zi Rong
ZR
Li
Wei Fen
WF
Niu
Dong
D
Dong
Niu
N
Lu
Ping
P
Fu
Ling Lin
LL
D018830:Adhesins, Escherichia coli / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D013294:Lactococcus lactis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D016019:Survival Analysis
D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D022361:Escherichia coli Vaccines; C494388:FaeG protein, E coli; D007074:Immunoglobulin G