Screening of specific internalization Fab fragment from human naive phage library by combinational bio-panning.
Phage display is a powerful tool in identifying specific antibody fragment binding to the target. Several strategies can be used to screen out a specific Fab from the phage library that binds to an antigen; it may be performed on immobilized targeted molecule, on the intact cells, or by other strategies. Antibodies with properties of recognizing tumor cell surface receptors in native conformation and ability to internalize to tumor cells through receptor binding are ideal carriers for targeted immuno-chemo and/or immuno-radiation therapy. This chapter describes a unique bio-panning method to screen out a Fab fragment against human epithelial growth factor receptor (EGFR) from a naive human Fab phage library for potential targeted therapeutic application on EGFR overexpressed cancers.
Wang
Xin
X
Department of Endocrinology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, China.
Cao
Brian B
BB
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002999:Clone Cells; D004705:Endocytosis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D066246:ErbB Receptors / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli; D005057:Eukaryotic Cells / Q000378:metabolism; D006378:Helper Viruses; D006801:Humans; D055767:Immobilized Proteins; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008967:Molecular Biology / Q000379:methods*; D019151:Peptide Library; D017434:Protein Structure, Tertiary; D017186:Titrimetry
D055767:Immobilized Proteins; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D066246:ErbB Receptors