title: [AQP1 expression in vascular endothelial cells in vitro under hypoxia conditions].
abstract: OBJECTIVE
  To investigate the expression of AQP1 in endothelial cells under hypoxia conditions in vitro.
  
  
  METHODS
  The cultured human umbilical vein endothelial cells were randomly assigned to hypoxia groups (exposed to 1% of O2 for 3 h, 6 h, 12 h and 24 h respectively), and control group (ordinary culture). The expressions of AQP1 mRNA and protein were detected by real time PCR and immunohistochemistry respectively.
  
  
  RESULTS
  The expressions of AQP1 protein and mRNA increased after exposure to hypoxia initially and then decreased afterwards.
  
  
  CONCLUSIONS
  Hypoxia regulates the expression of AQP1 in vascular endothelial cells.
authors.affiliation: Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qin-qin
authors.initials: QQ
authors.lastname: Wu
authors.forename: Yu-cheng
authors.initials: YC
authors.lastname: Chen
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Jiang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D014471:Umbilical Veins / Q000166:cytology*
chemical_list: C496020:AQP1 protein, human; D051398:Aquaporin 1