Photoprotective effect of Pothomorphe umbellata on UVB radiation-induced biomarkers involved in carcinogenesis of hairless mouse epidermis.
In this study we assessed the protective effect of topical application of Pothomorphe umbellata extract on ultraviolet B (UVB)-induced skin lesion parameters in hairless mouse epidermis. A single dose of UVB irradiation (0.23 kJ/m2) resulted in a significant decrease in thymine dimer-positive cells and apoptotic sunburn cells, with an increase in p53 and proliferating cell nuclear antigen-positive cells in the epidermis. After 5 weeks (total dose 13.17 kJ/m2) and 15 weeks (total dose 55.51 kJ/m2) of irradiation, P. umbellata treatment inhibited the hyperplasic response and induced an increase in p53-positive cells. These findings suggest that P. umbellata extract affords protection against UVB-induced skin lesions.
da Silva
Vanessa Vitoriano
VV
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brasil. vavitoriano@gmail.com
Ropke
Cristina Dislich
CD
Miranda
Denise Varella
DV
de Almeida
Rebeca Leite
RL
Sawada
Tânia Cristina Higashi
TC
Rivelli
Diogo Pineda
DP
Barros
Silvia Berlanga de Moraes
SB
D000279:Administration, Cutaneous; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015415:Biomarkers / Q000378:metabolism; D015197:Carcinogenicity Tests; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D005260:Female; D006965:Hyperplasia; D007150:Immunohistochemistry; D051379:Mice; D008812:Mice, Hairless; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D012878:Skin Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D014466:Ultraviolet Rays / Q000009:adverse effects*
D015415:Biomarkers; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents; D016159:Tumor Suppressor Protein p53