[Effects of uremia serum on monolayer permeability and F-actin in rat pulmonary microvascular endothelial cells].
OBJECTIVE
To observe the effects of uremia serum on monolayer permeability and F-actin in cultured rat pulmonary microvascular endothelial cell (RPMVEC) and to investigate the mechanism of injury of RPMVEC induced by uremia serum.


METHODS
RPMVEC was isolated and cultured from Wistar rat in vitro. The effects of serum of uremia patients on monolayer permeability of RPMVEC were observed with microfilter, and F-actin expression was evaluated by flow cytometry.


RESULTS
Serum of uremia patients induced the increased permeability of RPMVEC monolayer for 6 h and 12 h (P < 0.01), depolymerization of F-actin for 6 h and 12 h (P < 0.01), the increased permeability of RPMVEC monolayer induced by serum of uremia patients is significantly correlated with the depolymerization of F-actin (r = -0.927, P < 0.01).


CONCLUSIONS
Serum of uremia patients may induce the elevation of permeability monolayer and the depolymerization of F-actin in RPMVEC. The elevation of permeability monolayer induced by serum of uremia patients in RPMVEC is significantly correlated with the depolymerization of F-actin.
Wu
Sheng-Bin
SB
Department of Nephrology, Ninth People's Hospital, Shanghai Jiaotong University Medical College, Shanghai 200011, China.
Wang
Ying-Deng
YD
Zhang
Wei
W
D000199:Actins / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D008168:Lung / Q000098:blood supply; D008297:Male; D008875:Middle Aged; D051381:Rats; D017208:Rats, Wistar; D044967:Serum; D014511:Uremia / Q000097:blood*
D000199:Actins; D017077:Culture Media, Conditioned