Cloning and characterization of the biosynthetic gene cluster for tomaymycin, an SJG-136 monomeric analog.
Tomaymycin produced by Streptomyces achromogenes is a naturally produced pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. The gene cluster analysis reveals a novel biosynthetic pathway for the anthranilate moiety of PBDs. Gene replacement and chemical complementation studies were used to confirm the proposed biosynthetic pathway.
Li
Wei
W
Department of Chemistry and Biochemistry, Bldg. 091, University of Maryland, College Park, MD 20742, USA.
Chou
ShenChieh
S
Khullar
Ankush
A
Gerratana
Barbara
B
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001570:Benzodiazepinones / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001570:Benzodiazepinones; D004269:DNA, Bacterial; C003619:tomaymycin