Enhancement of erythromycin A production with feeding available nitrogen sources in erythromycin biosynthesis phase.
Effects of feeding different available nitrogen sources from 80 h in erythromycin biosynthesis phase on the erythromycin A (Er-A) production were investigated in 50 l fermenter. Feeding corn steep liquor and yeast extract, the Er-A production was enhanced, while the biotransformation from erythromycin C (Er-C) to Er-A had no increase. When ammonium sulphate was fed at high feeding rate, the maximal Er-A production and ratio of Er-A to Er-C were 7953 U/ml and 98.18:1 at 184 h, respectively, which were higher than that of the control (6742 U/ml and 5.47:1). The feeding ammonium sulphate process was successfully scaled up from 50 l to 25 m(3) fermenter. The maximal Er-A production reached 7938 U/ml at 203 h, which was enhanced by 22.1% compared with the control (6501 U/ml at 192 h). The ratio of Er-A to Er-C was 24.05:1, which was higher than that of the control (4.77:1).
Zou
Xiang
X
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
Hang
Hai-feng
HF
Chu
Ju
J
Zhuang
Ying-ping
YP
Zhang
Si-liang
SL
D000645:Ammonium Sulfate / Q000737:chemistry; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods; D004917:Erythromycin / Q000096:biosynthesis*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D013997:Time Factors
D004917:Erythromycin; D009584:Nitrogen; D010100:Oxygen; D000645:Ammonium Sulfate