title: Modified method for purifying rat liver branched-chain alpha-ketoacid dehydrogenase complex.
abstract: Branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) is a rate-limiting enzyme in the branched-chain amino acid catabolic pathway. We have developed a method of BCKDC purification from rat liver using hydrophobic interaction column chromatography (Shimomura et al., Arch. Biochem. Biophys., 283, 293-299 (1990)). Here we report a modification of the method designed to obtain the purified enzyme with high reproducibility.
authors.affiliation: Department of Materials Science and Engineering, Nagoya Institute of Technology, Showa-ku, Nagoya, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Fujimura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Keiichi
authors.initials: K
authors.lastname: Akita
authors.forename: Gustavo
authors.initials: G
authors.lastname: Bajotto
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Shimomura
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) / Q000302:isolation & purification*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D051381:Rats
chemical_list: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)