title: Effects of Pro-Arg, a novel dipeptide derived from protamine hydrolysate on H2O2-induced oxidative stress in human diploid fibroblasts.
abstract: Oxidative stress plays an important role in tissue damage associated with many different chronic degenerative diseases, such as cancer and cardiovascular disorders. In our previous study, a novel dipeptide Pro-Arg derived from protamine hydrolysates was found to possess significant antioxidative activity in vitro. In this study, we compared the antioxidant capabilities of purified and synthesized dipeptide (Pro-Arg) and found both of them had very strong hydroxyl-radical scavenging activity in vitro, even at a very low concentration. The protective effect of the dipeptide against oxidative stress was evaluated using H2O2-induced oxidative stress of human diploid fibroblasts MRC-5 cell model. Our results showed the dipeptide attenuated H2O2-induced cellular oxidative damage, and normalized S phase arrest of MRC-5 cells exposed to H2O2. Our findings demonstrate that Pro-Arg can protect against oxidative stress/damage and H2O2-induced human diploid fibroblasts cell death.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wang
authors.forename: Fengrong
authors.initials: F
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Fusen
authors.initials: F
authors.lastname: Han
authors.forename: Heyao
authors.initials: H
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004151:Dipeptides / Q000302:isolation & purification / Q000494:pharmacology*; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis; D018384:Oxidative Stress / Q000187:drug effects*; D011479:Protamines / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004151:Dipeptides; D016166:Free Radical Scavengers; D011479:Protamines; D017382:Reactive Oxygen Species; C045406:prolylarginine; D006861:Hydrogen Peroxide