title: CD3zeta expression and T cell proliferation are inhibited by TGF-beta1 and IL-10 in cervical cancer patients.
abstract: BACKGROUND
  Cervical cancer development from a squamous intraepithelial lesion is thought to be favored by an impaired T cell immunity. We evaluated parameters of T cell alterations such as proliferation, cytokine, and CD3zeta expression in peripheral blood and tumor-infiltrating T lymphocytes from women with squamous intraepithelial lesions (SIL) or cervical cancer (CC).
  
  
  CONCLUSIONS
  T cell proliferation and cytokine messenger RNA (mRNA) expression were similar in women with SIL and healthy donors, whereas low T cell proliferation and lower mRNA expression of IL-2, IL-10 and IFN-gamma were observed in women with CC. Moreover, infiltrating cells showed marginal responses. We also found that CD3zeta mRNA expression, whose protein is required for T cell activation, correlated with a decreased proliferation in advanced stages of the disease.
  
  
  CONCLUSIONS
  Experiments with T cells from healthy donors in the presence TGF-beta1 or IL-10 suggest that these cytokines have a relevant role in T cell responses during CC progression.
authors.affiliation: División de Infecciones Crónicas y Cáncer, Instituto Nacional de Salud Pública de México, Sta María Ahuacatitlán, 62508 Cuernavaca, Morelos, Mexico.
authors.forename: Cinthya E
authors.initials: CE
authors.lastname: Díaz-Benítez
authors.forename: Karla R
authors.initials: KR
authors.lastname: Navarro-Fuentes
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Flores-Sosa
authors.forename: Janet
authors.initials: J
authors.lastname: Juárez-Díaz
authors.forename: Felipe J
authors.initials: FJ
authors.lastname: Uribe-Salas
authors.forename: Edgar
authors.initials: E
authors.lastname: Román-Basaure
authors.forename: Ludwig E
authors.initials: LE
authors.lastname: González-Mena
authors.forename: Patricia
authors.initials: P
authors.lastname: Alonso de Ruíz
authors.forename: Guillermina
authors.initials: G
authors.lastname: López-Estrada
authors.forename: Alfredo
authors.initials: A
authors.lastname: Lagunas-Martínez
authors.forename: Victor H
authors.initials: VH
authors.lastname: Bermúdez-Morales
authors.forename: Juan M
authors.initials: JM
authors.lastname: Alcocer-González
authors.forename: Jesús
authors.initials: J
authors.lastname: Martínez-Barnetche
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Rosenstein
authors.forename: José
authors.initials: J
authors.lastname: Moreno
authors.forename: Vicente
authors.initials: V
authors.lastname: Madrid-Marina
mesh_terms: D000328:Adult; D000368:Aged; D017252:CD3 Complex / Q000096:biosynthesis* / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology*; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000276:immunology* / Q000494:pharmacology; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000276:immunology* / Q000473:pathology
chemical_list: D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D017077:Culture Media, Conditioned; C508609:IL10 protein, human; C508594:IL2 protein, human; D007376:Interleukin-2; D010835:Phytohemagglutinins; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D007371:Interferon-gamma