title: Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?
abstract: OBJECTIVE
  It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease.
  
  
  METHODS
  Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections.
  
  
  RESULTS
  Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn's disease, associated with emerging positivity of CD14(+) cells.
  
  
  CONCLUSIONS
  Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.
authors.affiliation: Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, Prague 4 142 20, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Frol'ová
authors.forename: Karel
authors.initials: K
authors.lastname: Smetana
authors.forename: Dana
authors.initials: D
authors.lastname: Borovská
authors.forename: Andrea
authors.initials: A
authors.lastname: Kitanovicová
authors.forename: Klára
authors.initials: K
authors.lastname: Klimesová
authors.forename: Ivana
authors.initials: I
authors.lastname: Janatková
authors.forename: Karin
authors.initials: K
authors.lastname: Malícková
authors.forename: Milan
authors.initials: M
authors.lastname: Lukás
authors.forename: Pavel
authors.initials: P
authors.lastname: Drastich
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Benes
authors.forename: Ludmila
authors.initials: L
authors.lastname: Tucková
authors.forename: Joachim C
authors.initials: JC
authors.lastname: Manning
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Helena
authors.initials: H
authors.lastname: Tlaskalová-Hogenová
mesh_terms: D000818:Animals; D015415:Biomarkers; D015153:Blotting, Western; D003092:Colitis / Q000139:chemically induced; D003093:Colitis, Ulcerative / Q000097:blood; D003106:Colon / Q000378:metabolism; D003424:Crohn Disease / Q000097:blood; D016264:Dextran Sulfate; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D037502:Galectin 3 / Q000097:blood*; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis; D037102:Lectins / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D037502:Galectin 3; D037102:Lectins; D018950:Lipopolysaccharide Receptors; D016264:Dextran Sulfate; D016650:Fluorescein-5-isothiocyanate