Proteomic analysis of changes induced by nonylphenol in Sprague-Dawley rat Sertoli cells.
Nonylphenol (NP) is a common environmental contaminant that is known to disrupt the reproductive system. The testicular Sertoli cells play a pivotal role in the regulation of spermatogenesis and are susceptible to NP-induced reproductive lesions. Our goal was to ascertain whether NP could induce apoptosis in Sertoli cells and to explore the preapoptotic changes in Sertoli cells at low NP concentrations, similar to environmental conditions. In order to survey events that occur at the protein level in Sertoli cells after exposure to NP, we used a proteomic approach with two-dimensional gel electrophoresis (2DE) and mass spectrometry to identify proteins with altered expression in rat Sertoli cells treated with 0.01 and 0.1 microM NP for 24 h. We separated 63 protein spots and identified 41 that were differently expressed in the NP-treated groups and the control. Of these 41 spots, we focused on Raf kinase inhibitor protein (RKIP), Annexin A7 (ANXA7), ERp57, and Peroxiredoxin 6 (PRDX6) for further analysis by Western blot. These proteins are involved in the response of Sertoli cells to programmed cell death. These data help to outline mechanisms by which NP might induce apoptotic tendencies in Sertoli cells.
Wu
Jiang
J
Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing, 210093, PR China.
Wang
Fuqiang
F
Gong
Yi
Y
Li
Dongmei
D
Sha
Jiahao
J
Huang
Xiaoyan
X
Han
Xiaodong
X
D000818:Animals; D017310:Annexin A7 / Q000378:metabolism; D017209:Apoptosis; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D052244:Endocrine Disruptors / Q000494:pharmacology* / Q000633:toxicity; D008297:Male; D013058:Mass Spectrometry; D054465:Peroxiredoxin VI / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology* / Q000633:toxicity; D049488:Phosphatidylethanolamine Binding Protein / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects*; D013091:Spermatogenesis
D017310:Annexin A7; D052244:Endocrine Disruptors; D010636:Phenols; D049488:Phosphatidylethanolamine Binding Protein; C025256:nonylphenol; D054465:Peroxiredoxin VI; C515984:Prdx6 protein, rat; C513196:PDIA3 protein, rat; D019704:Protein Disulfide-Isomerases