title: Speciation of glyphosate, phosphate and aminomethylphosphonic acid in soil extracts by ion chromatography with inductively coupled plasma mass spectrometry with an octopole reaction system.
abstract: Ion-pairing chromatography coupled with inductively coupled plasma mass spectrometry (ICP-MS) used for the speciation of phosphorus is limited as the mobile phase containing organic solvents changes in detection sensitivity and the carbon precipitates on torch and cones. To address this issue, anion-exchange chromatography with ICP-MS has been used for the speciation of glyphosate, phosphate and aminomethylphosphonic acid in soil extracts. The separation of the targets on a new column was achieved within 5 min using an eluent containing 20 mM NH(4)NO(3) at pH 5.1. Furthermore, since the polyatomic ions such as (14)N(16)O(1)H(+) and (15)N(16)O(+) from a nitrogen-based ion-pairing reagent interfered with ICP-MS detection of (31)P, an octopole reaction system was investigated to determine whether the polyatomic interferences could be reduced. The results show that addition of He to the cell can benefit analyses by reducing such interferences, but at the expense of reduced sensitivity. The detection limits in the range of 1.0-1.5 microg L(-1) (expressed as P) was achieved when 50 microL was injected using He as the collusion gas.
authors.affiliation: Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, SA 5095, Australia. zuliang.chen@unisa.edu.au
authors.forename: Zuliang
authors.initials: Z
authors.lastname: Chen
authors.forename: Wenxiang
authors.initials: W
authors.lastname: He
authors.forename: Michael
authors.initials: M
authors.lastname: Beer
authors.forename: Mallavarapu
authors.initials: M
authors.lastname: Megharaj
authors.forename: Ravendra
authors.initials: R
authors.lastname: Naidu
mesh_terms: D002845:Chromatography / Q000379:methods; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D006371:Helium; D007477:Ions; D007555:Isoxazoles; D013058:Mass Spectrometry / Q000379:methods; D063065:Organophosphonates / Q000032:analysis*; D010710:Phosphates / Q000032:analysis*; D012987:Soil / Q000032:analysis*; D013777:Tetrazoles; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006371:Helium; D007477:Ions; D007555:Isoxazoles; D063065:Organophosphonates; D010710:Phosphates; D012987:Soil; D013777:Tetrazoles; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid