title: Crystallization and preliminary X-ray analysis of the tumour necrosis factor alpha-tumour necrosis factor receptor type 2 complex.
abstract: Tumour necrosis factor receptor type 2 (TNFR2, TNFRSF1B) is an essential receptor for various host-defence functions of tumour necrosis factor alpha (TNF). As part of studies to determine the structure of TNFR2, the formation, crystallization and preliminary X-ray diffraction analysis of the TNF-TNFR2 complex are described. The TNF-TNFR2 complex, which comprises one TNF trimer and three TNFR2 monomers, was confirmed and purified by size-exclusion chromatography. Crystals of the TNF-TNFR2 complex were obtained using polyethylene glycol 3350 as a precipitant. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.5, b = 117.4, c = 246.8 A. Assuming the presence of two TNF-TNFR2 complexes in the asymmetric unit, the Matthews coefficient V(M) was 2.49 A(3) Da(-1) and the solvent content of the crystal was 50.7%. The crystal diffracted to 2.95 A resolution.
authors.affiliation: Osaka University, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mukai
authors.forename: Teruya
authors.initials: T
authors.lastname: Nakamura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kamada
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Yamagata
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tsutsumi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000032:analysis / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000737:chemistry*
chemical_list: D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha