title: Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors.
abstract: Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO(2) waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.
authors.affiliation: Center for Applied Near Field Optics Research (CAN-FOR), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.forename: Koichi
authors.initials: K
authors.lastname: Awazu
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimaki
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Ohki
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Komatsubara
authors.forename: Junji
authors.initials: J
authors.lastname: Tominaga
authors.forename: Penmetcha K R
authors.initials: PK
authors.lastname: Kumar
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D049329:Nanostructures / Q000032:analysis* / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
chemical_list: D012822:Silicon Dioxide