Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor.
A layer-by-layer route to prepare nanoporous Au film materials on transparent ITO substrates is reported by alternatively assembling Au and Ag nanoparticles through 1,5-pentanedithiol as a cross-linker, followed by that Ag nanoparticles are dissolved at room temperature in HAuCl4 solution. Electron transfer of cytochrome c (cyt. c) - an excellent model for investigation of biomolecules, is greatly facilitated at the nanoporous Au film with electron transfer rate constant (ks) of 3.9s(-1). Meanwhile, cyt. c adsorped onto the nanoporous Au film still maintain its enzymatic activity toward H2O2. On the basis of these experimental results, the cyt. c-nanoporous Au film is exploited to an amperometric biosensor for H2O2 with high selectivity, broad linear range, low detection limit, and long stability.
Zhu
Anwei
A
Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
Tian
Yang
Y
Liu
Haiqing
H
Luo
Yongping
Y
D015374:Biosensing Techniques / Q000295:instrumentation*; D045304:Cytochromes c / Q000502:physiology; D004579:Electron Transport; D006046:Gold; D006861:Hydrogen Peroxide / Q000032:analysis*; D008422:Materials Testing; D053758:Nanoparticles; D017971:Tin Compounds
D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold; D045304:Cytochromes c; D006861:Hydrogen Peroxide