title: [Differential incorporation of genomic segments into the influenza A virus reassortants in mixed infection].
abstract: The gene composition of the viral population obtained via mixed infection of embryonated chick eggs with influenza viruses at a high multiplicity of infection was analyzed. In mixed infection caused by influenza A/WSN/33 (H1N1) and A/Duck/Czechoslovakia/56 (H4N6) viruses, the population showed a preponderance of the reassortants whose content of genomic segments originating from either of the parent virus deviated strongly from the random pattern: the hemagglutinin (HA) gene of A/WSN/33 (H1N1) virus and the NP gene of A/Duck/Czechoslovakia/56 (H4N6) virus were prevalent in the gene composition of the reassortants. The mixed infection produced by influenza A/Udorn/72 (H3N2) virus and the reassortant R8 containing the HA gene of A/Duck/Ho Chi Minh/014/78 (H5N3) virus, the population of reassortants contained mainly the HA gene of A/Udorn/72 (H3N2) virus and the NP gene of the reassortant R8. The findings are discussed due to the problem of specific recognition of gene segments when incorporated into the viral particles.
authors.forename: N L
authors.initials: NL
authors.lastname: Varich
authors.forename: A K
authors.initials: AK
authors.lastname: Gitel'man
authors.forename: A A
authors.initials: AA
authors.lastname: Shilov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Smirnov
authors.forename: N V
authors.initials: NV
authors.lastname: Kaverin
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology; D002642:Chick Embryo; D016679:Genome, Viral / Q000235:genetics / Q000502:physiology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000502:physiology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000502:physiology; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics* / Q000502:physiology; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D005585:Influenza in Birds / Q000821:virology*; D016865:Reassortant Viruses / Q000235:genetics* / Q000502:physiology; D014758:Viral Core Proteins / Q000235:genetics / Q000502:physiology
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014758:Viral Core Proteins