Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived from glacial ice.
Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.
Simon
Carola
C
Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, Grisebachstr. 8, 37077 Göttingen, Germany.
Herath
Judith
J
Rockstroh
Stephanie
S
Daniel
Rolf
R
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005816:Genetic Complementation Test; D005858:Germany; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase