[Partial nucleotomy of the ovine disc as an in vivo model for disc degeneration].
OBJECTIVE
The aim of this study was to develop a suitable animal model for the clinical situation of progressive disc degeneration after microsurgical nucleotomy.


METHODS
Twenty sheep underwent standardised partial anterolateral nucleotomy at lumbar segment 3/4. After randomisation, 10 animals were sacrificed after 12 weeks (group 1). The remainder was sacrificed after 48 weeks (group 2). For radiological examination X-rays, MRI and post-mortem CT scans were performed. Lumbar discs L 3/4 with adjacent subchondral trabecular bone were harvested and analysed macroscopically and histologically. An image-analysing computer program was used to measure histomorphometric indices of bone structure.


RESULTS
17 segments could be evaluated. After 12 weeks (group 1) histological and radiological degenerative disc changes were noted. After 48 weeks (group 2), radiological signs in MRI reached statistical significance. Furthermore, group 2 showed significantly more osteophyte formations in CT scans. Histomorphometric changes of the disc and the adjacent vertebral bone structure suggest a significant progressive degenerative remodelling. The facet joints did not show any osteoarthrosis after 48 weeks.


CONCLUSIONS
Partial nucleotomy of the ovine lumbar disc leads to radiological and histological signs of disc degeneration similar to those seen in humans after microsurgical nucleotomy. The presented in vivo model may be useful to evaluate new orthopaedic treatment strategies.
Guder
E
E
Hals-Nasen-Ohrenheilkunde, Universitätsklinikum Rostock. ellen.guder@med.uni-rostock.de
Hill
S
S
Kandziora
F
F
Schnake
K J
KJ
D000818:Animals; D004195:Disease Models, Animal; D017586:Diskectomy; D005260:Female; D007091:Image Processing, Computer-Assisted; D007405:Intervertebral Disc Displacement / Q000473:pathology*; D008159:Lumbar Vertebrae / Q000473:pathology*; D008866:Microsurgery; D012756:Sheep; D013128:Spinal Osteophytosis / Q000473:pathology; D013167:Spondylitis, Ankylosing / Q000473:pathology; D055009:Spondylosis / Q000473:pathology*