Capillary lectin-affinity electrophoresis for glycan analysis.
Glycosylation is one of the most important post-translational events for proteins, affecting their functions in health and disease, and plays significant roles in various information trafficking for intercellular and intracellular biological events. The glycans which show such important effects are generally present as quite complex mixtures in minute amounts. The approach described here makes it possible to profile glycans for the analysis of post-translational modification of proteins with carbohydrates. The method is based on high-resolution separation of fluorescent-labeled carbohydrates by capillary electrophoresis with laser-induced fluorescent detection in the presence of carbohydrate-binding proteins at different concentrations. The technique affords simultaneous determination of glycans having similar structures even in complex mixtures.
Kakehi
Kazuaki
K
Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashi-Osaka, 577-8502, Japan. k_kakehi@phar.kindai.ac.jp
Kinoshita
Mitsuhiro
M
D000818:Animals; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism*
D005456:Fluorescent Dyes; D037102:Lectins; D011134:Polysaccharides