title: Developmental potential of human oocytes reconstructed by transferring somatic cell nuclei into polyspermic zygote cytoplasm.
abstract: The generation of patient-specific nuclear transfer embryonic stem cells holds huge promise in modern regenerative medicine and cell-based drug discovery. Since human in vivo matured oocytes are not readily available, human therapeutic cloning is developing slowly. Here, we investigated for the first time whether human polyspermic zygotes could support preimplantation development of cloned embryos. Our results showed that polyspermic zygotes could be used as recipients for human somatic cell nuclear transfer (SCNT). The preimplantation developmental potential of SCNT embryos from polyspermic zygotes was limited to the 8-cell stage. Since ES cell lines can be derived from single blastomeres, these results may have important significance for human ES cells derived by SCNT. In addition, confocal images demonstrated that all of the SCNT embryos that failed to cleave showed abnormal microtubule organization. The results of the present study suggest that polyspermic human zygotes could be used as a potential source of recipient cytoplasm for SCNT.
authors.affiliation: Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, Duobao Road #63, Guangzhou, Guangdong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.forename: Xinjie
authors.initials: X
authors.lastname: Chen
authors.forename: Yumei
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.forename: Shaoying
authors.initials: S
authors.lastname: Li
authors.forename: Yulin
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Sun
mesh_terms: D001755:Blastocyst / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D002675:Child, Preschool; D019976:Cloning, Organism / Q000379:methods*; D003593:Cytoplasm / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D008870:Microtubules; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology; D015053:Zygote / Q000502:physiology*