A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana x P. bolleana).
The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4 degrees C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 microM). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana x P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.
Guo
Xiao-Hong
XH
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, China.
Jiang
Jing
J
Lin
Shi-Jie
SJ
Wang
Bai-Chen
BC
Wang
Yu-Cheng
YC
Liu
Gui-Feng
GF
Yang
Chuan-Ping
CP
D000040:Abscisic Acid / Q000494:pharmacology; D017796:Caulimovirus / Q000235:genetics; D003080:Cold Temperature; D003890:Desiccation; D020869:Gene Expression Profiling; D009997:Osmotic Pressure; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D032107:Populus / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological; D032363:Tamaricaceae / Q000235:genetics*
D010937:Plant Growth Regulators; D000040:Abscisic Acid