title: Performance of a polymer-based DNA chip platform in detection and genotyping of human papillomavirus in clinical samples.
abstract: Human papillomavirus (HPV) plays a key role in the development of cervical and laryngeal cancers. The aim of our study was to compare the performance of a new hydrogel-based HPV genotyping biochip assay (Biochip) to a commercially available and CE-marked conventional PCR followed by reverse hybridization (GenID-PCR). One hundred twenty-three samples were available for the study. Of these samples, 101/123 were gynecological swabs, 8/123 were swabs or biopsy samples of genital warts, 7/123 were biopsy samples of otorhinolaryngeal lesions, 5/123 were samples of skin warts, and 2/123 were samples of orolabial abnormalities. These molecular methods for HPV genotyping showed comparable sensitivity and specificity. However, 19/123 of the results were discrepant. Specifically, Biochip showed better performance in the detection of multiple infections, especially when more than one high-risk genotype was present. Due to the different probe configurations used in the two assays, GenID-PCR achieves only group-specific detection of many HPV genotypes, whereas Biochip allows for specific identification. Overall, the newly developed HPV chip system (Biochip) proved to be a suitable tool for HPV detection and genotyping; it also proved to be superior for establishing HPV genotyping methods.
authors.affiliation: Department of Virology, University Medical Center Freiburg, Freiburg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Schenk
authors.forename: T
authors.initials: T
authors.lastname: Brandstetter
authors.forename: A
authors.initials: A
authors.lastname: Zur Hausen
authors.forename: J
authors.initials: J
authors.lastname: Alt-Mörbe
authors.forename: D
authors.initials: D
authors.lastname: Huzly
authors.forename: J
authors.initials: J
authors.lastname: Rühe
mesh_terms: D002584:Cervix Uteri / Q000821:virology; D005260:Female; D006801:Humans; D007830:Larynx / Q000821:virology; D009055:Mouth / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D012867:Skin / Q000821:virology