title: Hematopoietic stem cell transplantation without irradiation.
abstract: Hematopoietic stem cell (HSC) transplantation is limited by histocompatibility barriers and by lack of space in bone marrow niches. These obstacles prevent in vivo analysis of histoincompatible mutant stem cells and of HSC functions in non-irradiated mice. By genetically combining immunodeficiency with impaired function of the growth factor receptor Kit in mice, we generated a 'universal' HSC recipient that efficiently accepts long-term histocompatible and histoincompatible HSCs without prior irradiation.
authors.affiliation: Center for Regenerative Therapies Dresden, Dresden University of Technology, Germany. claudia.waskow@crt-dresden.de
authors.forename: Claudia
authors.initials: C
authors.lastname: Waskow
authors.forename: Vikas
authors.initials: V
authors.lastname: Madan
authors.forename: Susanne
authors.initials: S
authors.lastname: Bartels
authors.forename: Céline
authors.initials: C
authors.lastname: Costa
authors.forename: Rosel
authors.initials: R
authors.lastname: Blasig
authors.forename: Hans-Reimer
authors.initials: HR
authors.lastname: Rodewald
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000276:immunology*; D014916:Whole-Body Irradiation
chemical_list: D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; D019009:Proto-Oncogene Proteins c-kit