5-lipoxygenase-inhibitory constituents from Schizandra fructus and Magnolia flos.
In order to establish the antiallergic properties of Schisandra fructus and Magnolia flos, several compounds isolated from these plants were tested for 5-lipoxygenase (5-LOX) inhibitory activity in vitro, for the first time. The compounds including schizandrins, schisandrols, gomisins, fargesin, eudesmin and lirioresinol B dimethyl ether, inhibited 5-LOX-catalysed leukotriene production from A23187-treated rat basophilic leukemia (RBL-1) cells at concentrations of 1-100 microm. In particular, constituents such as schisandrol A and gomisins showed potent inhibitory activity (IC(50)s < 10 microm) on 5-LOX-catalysed leukotriene production, but were much less active on cyclooxygenase-2-catalysed prostaglandin E(2) and inducible nitric oxide-catalysed NO production. These compounds have the potential to be developed as novel antiallergic agents and may contribute to the antiallergic pharmacological use of these plant materials in Chinese medicine.
Lim
Hyun
H
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Son
Kun Ho
KH
Bae
Ki Hwan
KH
Hung
Tran Manh
TM
Kim
Yeong Shik
YS
Kim
Hyun Pyo
HP
D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D034242:Cyclooctanes / Q000302:isolation & purification / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D035264:Flowers; D005638:Fruit; D020128:Inhibitory Concentration 50; D015289:Leukotrienes / Q000096:biosynthesis; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry*; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D032141:Schisandra / Q000737:chemistry*
D018926:Anti-Allergic Agents; D034242:Cyclooctanes; D015289:Leukotrienes; D017705:Lignans; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; C520474:schisandrol A; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D015232:Dinoprostone