Prolyl-4-hydroxylase (AtP4H1) mediates and mimics low oxygen response in Arabidopsis thaliana.
Proline hydroxylation is an important phenomenon of a living cell. Prolyl-4-hydroxylases (P4H) responsible for this process have been characterized from animals, and one of its forms, HIF-P4H, is regarded as an oxygen sensor. In plants, P4H has been partially characterized from few species, and one of the Arabidopsis P4H (AtP4H1) has been shown to hydroxylate proline-rich peptides in vitro. In order to study its function in planta, we have overexpressed AtP4H1 in Arabidopsis. The AtP4H1oexp plants showed hypoxia-in-normoxia phenotype with strict requirement for carbon source for its growth, increased root hair, absence of trichome, and reduction in seed size. Genome-wide expression analyses suggest that expression of several genes related to hypoxia as well as plant growth and development are upregulated in AtP4H1oexp lines. Based on our studies on AtP4H1oexp lines, we speculate a direct role of AtP4H1 in hypoxia stress and in different stages of plant growth and development.
Asif
Mehar Hasan
MH
Plant Gene Expression Laboratory, National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, India.
Trivedi
Prabodh Kumar
PK
Misra
Prashant
P
Nath
Pravendra
P
D000818:Animals; D017360:Arabidopsis / Q000033:anatomy & histology / Q000201:enzymology* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006909:Hydroxyproline / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D046228:Microarray Analysis; D010100:Oxygen / Q000378:metabolism*; D011393:Procollagen-Proline Dioxygenase / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D014157:Transcription Factors; D011393:Procollagen-Proline Dioxygenase; D006909:Hydroxyproline; D010100:Oxygen