Rapid colorimetric field test to determine levels of deltamethrin on PermaNet surfaces: association with mosquito bioactivity.
OBJECTIVE
To evaluate a simple and inexpensive colorimetric test to measure the amount of cyanopyrethroid insecticide residue from filter paper exposed to mosquito net surfaces.


METHODS
The net sampling protocol and colorimetric test (NetTest) were evaluated for deltamethrin-impregnated PermaNet 2.0 by comparison with high-performance liquid chromatographic assays and mosquito mortality (WHO Cone Test).


CONCLUSIONS
The observed correlation between the amount of deltamethrin adsorbed onto the filter paper and the entire amount of deltamethrin per unit area of net material was good: 0.967, five assays. The relationship between the surface levels of deltamethrin determined by the colorimetric test and the 'gold standard' mosquito bioassay reveals a relatively accurate field test with a sensitivity of 91.4% and specificity if 85.4% (76 samplings from 19 nets).
Green
Michael D
MD
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. mdg4@cdc.gov
Atieli
Frances
F
Akogbeto
Martin
M
D000818:Animals; D000852:Anopheles; D001512:Bedding and Linens; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D008288:Malaria / Q000517:prevention & control; D009032:Mosquito Control / Q000379:methods*; D009570:Nitriles / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*
D009570:Nitriles; D010573:Pesticide Residues; D011722:Pyrethrins; C017180:decamethrin