Flow injection chemiluminescence sensor using molecularly imprinted polymers as recognition element for determination of maleic hydrazide.
A novel flow injection chemiluminescence (CL) sensor for the determination of maleic hydrazide (MH) using molecularly imprinted polymer (MIP) as recognition element is reported. The MH-MIP was synthesized by thermal initiated polymerization in methanol, using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker in the maleic hydrazide template molecule. Molecular modeling was employed to simulate the possible recognition process of the MIP, and to achieve high selectivity. Then the synthesized MH-MIP was employed as recognition element by packing into flow cell to establish a novel flow injection CL sensor. The CL intensity responded linearly to the concentration of MH in the range 3.5x10(-4)-5.0x10(-2) mg/mL with a detection limit of 6.0x10(-5) mg/mL (3sigma), which is lower than that of conventional methods. The relative standard deviation (RSD) for the determination of 1.0 microg/mL of MH was 2.7% (n=11). The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIP-CL sensor had been successfully applied to the determination of MH in vegetable samples.
Fang
Yanjun
Y
Institute of Hygienic and Environmental Medicinal Science, Tianjin 300050, PR China. yanjunfang1973@163.com
Yan
Shoulei
S
Ning
Baoan
B
Liu
Nan
N
Gao
Zhixian
Z
Chao
Fuhuan
F
D015374:Biosensing Techniques / Q000295:instrumentation*; D004784:Environmental Monitoring / Q000295:instrumentation*; D004785:Environmental Pollutants / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006540:Herbicides / Q000032:analysis; D008163:Luminescent Measurements / Q000295:instrumentation*; D008300:Maleic Hydrazide / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
D004785:Environmental Pollutants; D006540:Herbicides; D008300:Maleic Hydrazide