Circulating dendritic cells following burn.
Burns are associated with immune suppression and subsequent development of sepsis. Dendritic cells (DCs) are potent antigen-presenting cells that serve as a critical link between the innate and acquired immune systems, and are essential in coordinating the host response to pathogens. Using multicolour flow cytometry, the percentages of LIN(-) DR(+) CD11c(+) myeloid (mDC) and LIN(-) DR(+) CD123(+) plasmacytoid (pDC) subsets were determined in peripheral blood from 32 people (15 septic and 5 non-septic burn victims and 12 age- and gender-matched healthy controls, up to 20 days from injury). Analysis revealed significant reductions in circulating mDCs and pDCs in survivor as well as non-survivor septic cases compared with non-septic cases and controls (p<0.001). These findings suggest that deficiencies in mDCs and pDC subsets are related to sepsis following severe burn, and may contribute to immunosuppression among burn victims.
D'Arpa
N
N
Plastic Surgery and Burns Therapy Operating Unit, ARNAS, Civic Hospital, Piazza Leotta 4, 90127 Palermo, Italy. lab.biotecno@ospedalecivicopa.org
Accardo-Palumbo
A
A
Amato
G
G
D'Amelio
L
L
Pileri
D
D
Cataldo
V
V
Mogavero
R
R
Lombardo
C
C
Napoli
B
B
Conte
F
F
D000328:Adult; D000367:Age Factors; D000938:Antigen-Presenting Cells / Q000166:cytology* / Q000276:immunology; D002056:Burns / Q000276:immunology* / Q000401:mortality; D016022:Case-Control Studies; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology*; D018805:Sepsis / Q000276:immunology* / Q000401:mortality