title: A combination of molecular probe-tandem electrospray ionization mass spectrometry: a technique for tracing structural changes in phospholipid hydroperoxides.
abstract: An ethyl-labeled phosphatidylcholine hydroperoxide (PC-OOH/Et 2) was synthesized as a molecular probe for naturally occurring PC-OOH 1. Applying the precursor ion scan mode in tandem ESI mass spectrometry at m/z 198, a signal of the PC-OOH/Et 2 alone could be selectively detected even in the presence of a large excess of a complex mixture of phospholipids in the blood. Furthermore, molecular species that formed from PC-OOH/Et 2 by its degradation in the blood were also observed in the same spectrum. Since the molecular probe-and-mass spectrometry-assisted analytical method presented herein requires no separation process by HPLC or TLC and is speedy, requiring less than 1 h, it may be useful in lipid analysis.
authors.affiliation: Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka, Okayama, Japan.
authors.forename: Rumiko
authors.initials: R
authors.lastname: Shimizu
authors.forename: Ai
authors.initials: A
authors.lastname: Nagai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tominaga
authors.forename: Megumi
authors.initials: M
authors.lastname: Imura
authors.forename: Arnold N
authors.initials: AN
authors.lastname: Onyango
authors.forename: Minoru
authors.initials: M
authors.lastname: Izumi
authors.forename: Shuhei
authors.initials: S
authors.lastname: Nakajima
authors.forename: Shoichi
authors.initials: S
authors.lastname: Tahara
authors.forename: Takao
authors.initials: T
authors.lastname: Kaneko
authors.forename: Naomichi
authors.initials: N
authors.lastname: Baba
mesh_terms: D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D015335:Molecular Probes; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide