title: Molecular genetic characterization of the merozoite surface protein 1 Gene of Plasmodium vivax from reemerging Korean isolates.
abstract: Plasmodium vivax merozoite surface protein 1 (PvMSP-1) has been considered a major candidate for the development of an antimalaria vaccine, but the molecule exhibits antigenic diversity among isolates. The extent of genetic polymorphism in the region between interspecies conserved blocks 4 and 5 (ICB4 and ICB5) of the PvMSP-1 gene was analyzed for 30 Korean isolates. Two genotypes, SK-A and SK-B, were identified on the basis of amino acid substitution. Almost all the amino acid sequences of the Korean isolates were nearly identical to those of the Solomon Island isolate Solo-83 (97.8 to 99.9% similarity) and Philippine isolates Ph-79, Ph-52-2, and Ph-49 (97.3 to 99.8% similarity). Also, we report two sequences in the isolates that were characterized on the basis of restriction fragment length polymorphism (RFLP). The RFLP profiles following digestion with the DraI restriction enzyme produced two distinguishable patterns. This study might be the first report of the region between ICB4 and ICB5 of the MSP-1 gene of P. vivax in South Korea.
authors.affiliation: Department of Malariology, Paik Institute for Clinical Research, Inje University College of Medicine, Jin-gu, Busan, South Korea.
authors.forename: So-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Seung-Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Shin
authors.forename: Chi-Sook
authors.initials: CS
authors.lastname: Moon
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Weon-Gyu
authors.initials: WG
authors.lastname: Kho
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution; D000818:Animals; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D007723:Korea / Q000453:epidemiology; D016780:Malaria, Vivax / Q000453:epidemiology* / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D020066:Merozoite Surface Protein 1