Modified method for purifying rat liver branched-chain alpha-ketoacid dehydrogenase complex.
Branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) is a rate-limiting enzyme in the branched-chain amino acid catabolic pathway. We have developed a method of BCKDC purification from rat liver using hydrophobic interaction column chromatography (Shimomura et al., Arch. Biochem. Biophys., 283, 293-299 (1990)). Here we report a modification of the method designed to obtain the purified enzyme with high reproducibility.
Fujimura
Yukihiro
Y
Department of Materials Science and Engineering, Nagoya Institute of Technology, Showa-ku, Nagoya, Japan.
Muramatsu
Yuji
Y
Akita
Keiichi
K
Bajotto
Gustavo
G
Shimomura
Yoshiharu
Y
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) / Q000302:isolation & purification*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology*; D051381:Rats
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)