title: [Poly-aspartamide-glutamic acid grafted low molecular weight polyethylenimine as a novel non-viral gene vector].
abstract: To develop a novel gene delivery vector with poly-aspartamide-glutamic acid and polyethylenimine as the backbone.
  
  
  
  alpha, beta-poly-(N-2-hydroxypropyl)-D, L-aspartamide-glutamic acid (PHPAG) was synthesized and low molecular weight polyethylenimine (PEI 1.8 kDa) was grafted to form PHPAG-PEI 1800. Chemical and biological characterization of the polymer was identified.
  
  
  
  The polymer was confirmed by (1)H-NMR, and the molecular weight was about 1.2 x 10(4). The ability of DNA binding was showed by gel retardation assay at N/P ratio of 3. 5. MTT assay showed that the polymer was non toxic in COS-7 and A293 cell lines. In vitro test demonstrated that it had high transfection efficiency in B16 and Hela cell lines.
  
  
  
  PHPAG-PEI 1800 was successfully synthesized,which might be a potential vector for gene delivery.
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: He
authors.forename: Dan-Jun
authors.initials: DJ
authors.lastname: Zhao
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Jiang
authors.forename: Zhong-Ren
authors.initials: ZR
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Wang
authors.forename: Gu-Ping
authors.initials: GP
authors.lastname: Tang
mesh_terms: D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D018698:Glutamic Acid / Q000737:chemistry*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C071943:polyaspartylglutamate; C017645:polyaspartate; D018698:Glutamic Acid; D011094:Polyethyleneimine