Production of glycolipid biosurfactants, mannosylerythritol lipids, by a smut fungus, Ustilago scitaminea NBRC 32730.
A smut fungus Ustilago scitaminea NBRC 32730 on sugar cane (Saccharum) was found to accumulate a large amount of glycolipids in the culture medium. As a result of structural characterization, the main glycolipid was identified as MEL-B, 4-O-beta-(2',3'-di-O-alka(e)noyl-6'-O-acetyl-D-mannopyranosyl)-erythritol. The MEL-B was sufficiently produced from a variety of sugars such as sucrose, glucose, fructose, and mannose. Olive oil and methyl oleate were also available as carbon sources to produce MEL-B. However, these residual oils made product recovery very complicated. Under optimal conditions, a maximum MEL yield of 12.8 g/l was achieved by feeding of sucrose.
Morita
Tomotake
T
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
Ishibashi
Yuko
Y
Fukuoka
Tokuma
T
Imura
Tomohiro
T
Sakai
Hideki
H
Abe
Masahiko
M
Kitamoto
Dai
D
D003470:Culture Media / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism*; D014588:Ustilago / Q000378:metabolism*
D003470:Culture Media; D006017:Glycolipids; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; D013395:Sucrose