Effect of quercetin on oxidative nuclear and mitochondrial DNA damage.
Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability.
Potenza
Lucia
L
Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino "Carlo Bo", Urbino, Italy. lucia.potenza@uniurb.it
Calcabrini
Cinzia
C
De Bellis
Roberta
R
Mancini
Umberto
U
Cucchiarini
Luigi
L
Dachà
Marina
M
D000975:Antioxidants / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects / Q000528:radiation effects; D008928:Mitochondria / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011794:Quercetin / Q000494:pharmacology*; D014466:Ultraviolet Rays
D000975:Antioxidants; D002839:Chromans; D004272:DNA, Mitochondrial; D011794:Quercetin; D006861:Hydrogen Peroxide; D006899:Mixed Function Oxygenases; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid