title: Stochastically determined directed movement explains the dominant small-scale mitochondrial movements within non-neuronal tissue culture cells.
abstract: The apparently stationary phase of mitochondrial motion was investigated in epithelial cells by spinning disk confocal light microscopy combined with image correlation based single particle tracking using custom software producing sub-pixel accuracy measurements (approximately 5 nm) at 10-12 Hz frame-rates. The analysis of these data suggests that the previously described stationary, or anchored phase, in mitochondrial movement actually comprise Brownian diffusion, interspersed with frequent and brief motor-driven events whose duration are stochastically determined. We have therefore discovered a new aspect of mitochondrial behavior, which we call stochastically determined, directed movement.
authors.affiliation: Biophysical Sciences Institute, Department of Physics, South Road, Durham University, Durham DH1 3LE, UK.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Saunter
authors.forename: Ming Der
authors.initials: MD
authors.lastname: Perng
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Love
authors.forename: Roy A
authors.initials: RA
authors.lastname: Quinlan
mesh_terms: D000818:Animals; D002460:Cell Line; D004173:Dipodomys; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000502:physiology*; D013269:Stochastic Processes; D046509:Tissue Culture Techniques