miRNA expression profiles in head and neck squamous cell carcinoma and adjacent normal tissue.
BACKGROUND
The expression of miRNA in head and neck squamous cell carcinomas (HNSCCs) that had been classified as high risk by surgical pathologic features and validated by trial outcome for disease recurrence was determined and compared with matched adjacent normal tissues.


METHODS
miRNA and corresponding gene expression were determined using miRNA bioarrays and gene expression arrays.


RESULTS
Twenty miRNAs were determined to be differentially regulated in the HNSCC samples when compared with their normal tissue counterparts. Quantitative reverse transcriptase-polymerase chain reaction confirmed differential regulation of miRNA expression, and gene expression analysis on these same-paired samples confirmed the loss of putative mRNA targets including genes such as adenomatous polyposis coli, programmed cell death protein 4, and TGF beta receptor 3 in the tumor samples.


CONCLUSIONS
These data suggest a role for the upregulation of specific miRNAs in high-risk HNSCC. Furthermore, upregulation of these miRNAs may be responsible for the elimination of mRNAs that lead to the growth and progression of HNSCC.
Ramdas
Latha
L
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Giri
Uma
U
Ashorn
Cheryl L
CL
Coombes
Kevin R
KR
El-Naggar
Adel
A
Ang
K Kian
KK
Story
Michael D
MD
D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D019744:Chemokines, C / Q000235:genetics; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D054464:Peroxiredoxins / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050396:TRPP Cation Channels / Q000235:genetics; D015854:Up-Regulation
D025601:Adenomatous Polyposis Coli Protein; D051017:Apoptosis Regulatory Proteins; D019744:Chemokines, C; D035683:MicroRNAs; D054464:Peroxiredoxins; D016601:RNA-Binding Proteins; D018125:Receptors, Transforming Growth Factor beta; D050396:TRPP Cation Channels; C114487:PDCD4 protein, human; C092390:XCL1 protein, human; C515980:PRDX4 protein, human