title: An alternative and fast method for determination of glyphosate and aminomethylphosphonic acid (AMPA) residues in soybean using liquid chromatography coupled with tandem mass spectrometry.
abstract: A simple and specific method using reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was investigated, which allowed the determination of residues of glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), in soybean samples. An aqueous extraction with liquid-liquid partition followed by protein precipitation was performed before the LC/MS/MS determination. The quantitation of glyphosate and AMPA was performed in positive and negative ESI mode, respectively, using the multiple reaction monitoring (MRM) mode with three transitions for each analyte to enhance the specificity of the method and avoid false positives. The methodology reported in this work is capable of detecting residues of glyphosate and AMPA in soybean samples with limits of quantification of 0.30 and 0.34 mg kg(-1), respectively. This alternative method has throughput advantages such as simpler sample preparation and faster chromatographic analysis.
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares, Av. Prof. Lineu Prestes 2242, Cidade Universitária, São Paulo, Brazil.
authors.forename: Helio A
authors.initials: HA
authors.lastname: Martins-Júnior
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Lebre
authors.forename: Alexandre Y
authors.initials: AY
authors.lastname: Wang
authors.forename: Maria A F
authors.initials: MA
authors.lastname: Pires
authors.forename: Oscar V
authors.initials: OV
authors.lastname: Bustillos
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D006540:Herbicides / Q000032:analysis*; D007555:Isoxazoles; D063065:Organophosphonates / Q000032:analysis*; D015203:Reproducibility of Results; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013777:Tetrazoles; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D007555:Isoxazoles; D063065:Organophosphonates; D013777:Tetrazoles; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid