Induction of Th1-type immune response by chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen Der p 2 for oral vaccination in mice.
This study was to prepare the chitosan-pDer p 2 nanoparticles and to investigate the effect of chitosan-DNA nanoparticles on immune response in mice by oral delivery of chitosan-DNA nanoparticles. The nanoparticles were synthesized by complexing chitosan with plasmid DNA. The DNA was fully complexed into chitosan-DNA nanoparticles, suggesting a 100% encapsulation efficiency. Chitosan-DNA complex renders a significant protection of the plasmid. No effect on cell viability was observed in both cell types and average cell viability over 100% was obtained. Oral gene delivery with chitosan-DNA nanoparticles can generate a higher level expression of gene in vivo. Oral chitosan-pDer p 2 nanoparticles in BALB/c mice can induce IFN-gamma in serum and prevent subsequent sensitization of Th2 cell-regulated specific IgE responses. The data indicate that the oral administration of chitosan-pDer p 2 nanoparticles results in the expression of Der p 2 in the epithelial cells of both stomach and small intestine and the induction of Th1-type immune response.
Li
Guo Ping
GP
Inflammation & Allergic Disease Lab, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan, China.
Liu
Zhi Gang
ZG
Liao
Bin
B
Zhong
Nan Shan
NS
D000284:Administration, Oral; D000485:Allergens / Q000235:genetics / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology*; D060829:Arthropod Proteins; D048271:Chitosan / Q000008:administration & dosage / Q000276:immunology; D015870:Gene Expression; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D006967:Hypersensitivity / Q000628:therapy*; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007421:Intestine, Small / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage; D010957:Plasmids / Q000008:administration & dosage / Q000276:immunology; D013270:Stomach / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use
D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D019444:Vaccines, DNA; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D048271:Chitosan