[Study on the adsorption of endotoxin and proinflammatory cytokines with HB-H-7 resin in vitro].
OBJECTIVE
To study the efficacy and safety of adsorption of endotoxin and proinflammatory cytokines with HB-H-7 resin in vitro.


METHODS
Static adsorption experiment: HB-H-7 resin was added into plasma of endotoxin-positive patients according to the ratio of 1;10. Then, they were put in a constant temperature water bath oscillator, and oscillated for 2 hours. Before and after the experiment, plasma endotoxin, proinflammatory cytokines, protein and electrolytes were detected, and the rate of 2-hour absorption was calculated. The experiment was repeated 10 times. Dynamic adsorption experiment: 5 ml resin was put into a self-made perfusion unit. Endotoxin-positive patients plasma (50 ml) was perfused for 2 hours. The flow rate of plasma was controlled at 4 ml/min with the infusion pump, and the plasma endotoxin was determined at 0, 30, 60 and 120 minutes after plasma hemoperfusion, and the absorption rates were calculated. The results were compared with static adsorption. The influence of temperature during adsorption was determined as follows. Perfusion method was similar with dynamic adsorption experiment. The perfusion units were either placed in a 37 centigrade water path or 25 centigrade (room temperature). Then, the plasma endotoxin was measured 2 hours after plasma perfusion, and the absorption rates were calculated.


RESULTS
In static adsorption experiment, the plasma endotoxin and proinflammatory cytokines were significantly lower after adsorption with HB-H-7 resin adsorption (all P<0.05). The adsorption rates of endotoxin, tumor necrosis factor-alpha and interleukin-1, 6, 8 were 99.2%, 55.0%, 57.0%, 75.0%, 42.0%, respectively. Changes in protein were small (all P<0.05) , and there was no significant change in plasma electrolytes (all P>0.05). Dynamic adsorption rate was higher than that of static, but the differences were not significant (99.8% vs. 99.1%, P>0.05). There was no statistically significant difference between difference in temperature (37 centigrade vs. 25 centigrade, 99.8% vs. 99.9%, P>0.05).


CONCLUSIONS
HB-H-7 resin effectively absorbs endotoxin and proinflammatory cytokines in vitro. Its adsorption rate for protein is lower, and it has no obvious effects on electrolytes.
Tian
Shuang
S
Third Central Clinical Hospital of Tianjin Medical University, Tianjin 300170, China.
Li
Tao
T
Luo
Li-qun
LQ
Du
Zhi
Z
D000327:Adsorption; D016207:Cytokines / Q000097:blood*; D004731:Endotoxins / Q000097:blood*; D006464:Hemoperfusion; D006801:Humans; D066298:In Vitro Techniques; D012117:Resins, Synthetic
D016207:Cytokines; D004731:Endotoxins; D012117:Resins, Synthetic