[Characteristics of mRNA level of interleukin 12 and intercellular adhesion molecule 1 and monocyte chemotactic protein 3 in human nasal epithelial cells].
OBJECTIVE
To investigate the mRNA level of IL-12, ICAM-1 and MCP-3 in human nasal epithelial cells.


METHODS
Firstly, human primary nasal epithelial cells were cultured, and then 4 pairs of primers were designed for detecting mRNA level of IL-12, ICAM-1 and MCP-3. The 938 bp PCR products of GAPDH were used as internal standards. The mRNA expression levels of IL-12, ICAM-1 and MCP-3 in primary nasal epithelial cells was measured with semi-quantitative reverse transcription-polymerase chain reaction.


RESULTS
The round or irregular primary nasal epithelial cells were observed sticking to the bottom of cell culture plates under 400 times optical microscope. The expressions of IL-12 p35, ICAM-1 and MCP-3 mRNA were found in primary nasal epithelial cells while IL-12 p40 subunit was not detected.


CONCLUSIONS
IL-12 p35, ICAM-1 and MCP-3 mRNAs are expressed in primary nasal epithelial cells, whereas effective IL-12 with integrity is not present in nasal epithelial cells.
Zhu
Xuewei
X
Department of Otolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
Zhu
Dongdong
D
Sun
Kewei
K
Dong
Zhen
Z
D002478:Cells, Cultured; D054410:Chemokine CCL7 / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D053765:Interleukin-12 Subunit p35 / Q000378:metabolism*; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
C515450:CCL7 protein, human; D054410:Chemokine CCL7; C508858:IL12A protein, human; D053765:Interleukin-12 Subunit p35; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1