title: [Lentinan-graft-polyethylenimine-a novel vector for gene delivery].
abstract: To develop a novel vector for gene delivery with low molecular weight polyethylenimine grafted to the natural polysaccharide and conjugated to folic acid (LNT-PEI-FA).
  
  
  
  The properties of LNT-PEI-FA were characterized by (1)H-NMR, FT-IR and TGA, respectively. The particle size of LNT-PEI-FA/DNA complex was measured. The DNA binding ability of LNT-PEI-FA was detected by gel electrophoresis retardation assay.
  
  
  
  The particle size of LNT-PEI-FA/DNA complex was about 200 nm. Gel electrophoresis showed that at N/P ratio of 1.8 (W/W) the polymer was able to completely condense DNA. In vitro experiments showed a high efficiency of gene transfection in A293 and B16 cell lines.
  
  
  
  A novel non-viral vector LNT-PEI-FA was successfully synthesized and characterized, which may be applied in gene transfection research in the future.
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Jiang
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Hu
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Dan-Jun
authors.initials: DJ
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Wang
authors.forename: Gu-Ping
authors.initials: GP
authors.lastname: Tang
mesh_terms: D002460:Cell Line; D005492:Folic Acid / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D007912:Lentinan / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D007912:Lentinan; D011094:Polyethyleneimine; D005492:Folic Acid