title: [Study on the specific immunity of dendritic cell vaccine loading human umbilical vein endothelial cell antigen against U14 cervical cancer].
abstract: OBJECTIVE
  To explore the specific immunity of dendritic cell (DC) vaccine loading human umbilical vein endothelial cell (HUVEC) antigen.against U14 cervical cancer of mice.
  
  
  METHODS
  Primary HUVECs were cultured, identified and made into antigen. The BALB/c mice were immunized with DCs loading HUVEC antigen 4 times a week. One week after last vaccination, U14 cervical cancer cells were injected subcutaneously into the mice. The tumor size, CTL response of spleen lymphocytes in vitro, the percentage of CD3(+)CD8(+) surface markers of spleen lymphocytes, and the titer of serum antibody were detected. The specific immunity was examined by immunocytochemistry and Western blot.
  
  
  RESULTS
  HUVECs with high purity were successfully cultured and by identified by immunocytochemistry and RT-PCR. After the vaccine was injected into mice, the tumors of mice in PBS group grew faster than the those in other groups. The tumors of mice in HUVEC-DC groups grew slowly and disappeared after 2 weeks and The tumors of mice in DC group disappeared after 3 weeks. The CTL response of spleen lymphocytes in vitro showed that HUVEC-DC-T cells could kill HUVEC cells. The percentage of CD3(+)CD8(+) surface markers of spleen lymphocytes in HUVEC-DC group was higher than that in other groups. The titer of serum antibody was 1:800. immunocytochemistry analysis indicated HUVEC-DC group had specific antigen-antibody reaction to HUVECs through and Western blot analysis revealed there were specific bands at 130 KDa and 220 KDa.
  
  
  CONCLUSIONS
  HUVEC-DC vaccine and DC vaccine can inhibit the tumor growth of U14 cervical cancer of mice. The special cellular and humoral immunity are induced by HUVEC-DC vaccine. Furthermore, some antigens in HUVECs maybe have special immune reaction with integrin alphav and VEGF-R2.
authors.affiliation: Department of Pathophysiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. zhaojun_2@yahoo.com.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Jing-ming
authors.initials: JM
authors.lastname: Zhai
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Yang
authors.forename: You-tian
authors.initials: YT
authors.lastname: Huang
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Zi-ming
authors.initials: ZM
authors.lastname: Dong
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D015153:Blotting, Western; D040881:CD11a Antigen / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D015820:Cadherins / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D042783:Endothelial Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D016219:Immunotherapy, Adoptive; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy; D016896:Treatment Outcome; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D014841:von Willebrand Factor / Q000276:immunology; D000099152:Cadherin 5
chemical_list: D000941:Antigens; D015703:Antigens, CD; D051940:B7-2 Antigen; D040881:CD11a Antigen; D017252:CD3 Complex; D015820:Cadherins; D019496:Cancer Vaccines; D007106:Immune Sera; D014841:von Willebrand Factor; D000099152:Cadherin 5