title: Potential of DNA sequences to identify zoanthids (Cnidaria: Zoantharia).
abstract: The order Zoantharia is known for its chaotic taxonomy and difficult morphological identification. One method that potentially could help for examining such troublesome taxa is DNA barcoding, which identifies species using standard molecular markers. The mitochondrial cytochrome oxidase subunit I (COI) has been utilized to great success in groups such as birds and insects; however, its applicability in many other groups is controversial. Recently, some studies have suggested that barcoding is not applicable to anthozoans. Here, we examine the use of COI and mitochondrial 16S ribosomal DNA for zoanthid identification. Despite the absence of a clear barcoding gap, our results show that for most of 54 zoanthid samples, both markers could separate samples to the species, or species group, level, particularly when easily accessible ecological or distributional data were included. Additionally, we have used the short V5 region of mt 16S rDNA to identify eight old (13 to 50 years old) museum samples. We discuss advantages and disadvantages of COI and mt 16S rDNA as barcodes for Zoantharia, and recommend that either one or both of these markers be considered for zoanthid identification in the future.
authors.affiliation: Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan. fredsinniger@hotmail.com
authors.forename: Frederic
authors.initials: F
authors.lastname: Sinniger
authors.forename: James D
authors.initials: JD
authors.lastname: Reimer
authors.forename: Jan
authors.initials: J
authors.lastname: Pawlowski
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D003063:Cnidaria / Q000145:classification* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV