title: Genotoxic hazard and cellular stress in pediatric patients treated for psoriasis with the Goeckerman regimen.
abstract: The Goeckerman regimen (GR) represents a local treatment of psoriasis and includes topical dermal application of crude coal tar (containing polycyclic aromatic hydrocarbons) and exposure to UV-irradiation. The aim of the study was to evaluate contribution of GR to genotoxic risk and cellular stress in pediatric patients who represent a sensitive population subgroup. Genotoxic risk (42 patients) was evaluated by using chromosomal aberrations (CA) in peripheral lymphocytes. Cellular stress (26 patients) was assessed by using heat shock proteins (Hsp70). All indicators were determined in blood samples collected before GR and immediately after GR. Decreasing of psoriasis area and severity index (PASI) score indicated higher likelihood of GR (p < 0.001). Significantly increased CA (p < 0.001) and Hsp70 (p < 0.05) indicated higher genotoxic risk and cellular stress in sensitive pediatric patients, immediately after GR.
authors.affiliation: Faculty of Medicine in Hradec Kralove, Institute of Pathological Physiology, Charles University in Prague, Simkova, Hradec Kralove, Czech Republic. borka@lfhk.cuni.cz
authors.forename: Lenka
authors.initials: L
authors.lastname: Borska
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
authors.forename: Kvetoslava
authors.initials: K
authors.lastname: Hamakova
authors.forename: Jan
authors.initials: J
authors.lastname: Kremlacek
authors.forename: Karel
authors.initials: K
authors.lastname: Ettler
authors.forename: Sajith
authors.initials: S
authors.lastname: Vellappally
authors.forename: Jacob
authors.initials: J
authors.lastname: Vimal
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Fiala
mesh_terms: D000287:Administration, Topical; D000293:Adolescent; D002648:Child; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003033:Coal Tar / Q000008:administration & dosage / Q000633:toxicity*; D003131:Combined Modality Therapy; D018153:Czech Republic; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007641:Keratolytic Agents / Q000008:administration & dosage / Q000633:toxicity*; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D008297:Male; D011565:Psoriasis / Q000188:drug therapy* / Q000453:epidemiology / Q000532:radiotherapy*; D012307:Risk Factors; D012720:Severity of Illness Index; D013312:Stress, Physiological / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007641:Keratolytic Agents; D003033:Coal Tar