Morphological analysis of the antimicrobial action of nitric oxide on gram-negative pathogens using atomic force microscopy.
Atomic force microscopy (AFM) was used to study the morphological changes of two gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli, after exposure to nitric oxide (NO). The time-dependent effects of NO released from a xerogel coating and the concentration-dependent effects rendered by a small molecule that releases NO in a bolus were examined and compared. Bacteria exhibited irregular and degraded exteriors. With NO-releasing surfaces, an increase in surface debris and disorganized adhesion patterns were observed compared to controls. Analysis of cell surface topography revealed that increasing membrane roughness correlated with higher doses of NO. At a lower total dose, NO delivered via a bolus resulted in greater membrane roughness than NO released from a surface via a sustained flux. At sub-inhibitory levels, treatment with amoxicillin, an antibiotic known to compromise the integrity of the cell wall, led to morphologies resembling those resulting from NO treatment. Our observations indicate that cell envelope deterioration is a visible consequence of NO-exposure for both gram-negative species studied.
Deupree
Susan M
SM
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Schoenfisch
Mark H
MH
D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry; D002462:Cell Membrane / Q000187:drug effects; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D009569:Nitric Oxide / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000187:drug effects*; D013499:Surface Properties / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D009569:Nitric Oxide; D000658:Amoxicillin