title: Binding of CCCTC-binding factor in vivo to the region located between Rep* and the C promoter of Epstein-Barr virus is unaffected by CpG methylation and does not correlate with Cp activity.
abstract: In this study, the binding of the insulator protein CCCTC-binding factor (CTCF) to the region located between Rep* and the C promoter (Cp) of Epstein-Barr virus (EBV) was analysed using chromatin immunoprecipitation and in vivo footprinting. CTCF binding was found to be independent of Cp usage in cell lines corresponding to the major EBV latency types. Bisulfite sequencing and an electrophoretic mobility-shift assay (using methylated and unmethylated probes) revealed that CTCF binding was insufficient to induce local CpG demethylation in certain cell lines and was unaffected by CpG methylation in the region between Rep* and Cp. In addition, CTCF binding to the latency promoter, Qp, did not correlate with Qp activity.
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Daniel
authors.initials: D
authors.lastname: Salamon
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Banati
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Koroknai
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Mate
authors.initials: M
authors.lastname: Ravasz
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Kalman
authors.initials: K
authors.lastname: Szenthe
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Bathori
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Bakos
authors.affiliation: Department of Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss Allee 11, D-93053 Regensburg, Germany.
authors.forename: Hans Helmut
authors.initials: HH
authors.lastname: Niller
authors.affiliation: Department of Microbiology and Hygiene, University of Regensburg, Franz-Josef-Strauss Allee 11, D-93053 Regensburg, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Wolf
authors.affiliation: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors.forename: Janos
authors.initials: J
authors.lastname: Minarovits
mesh_terms: D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D018899:CpG Islands / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology*; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D017735:Virus Latency
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D012097:Repressor Proteins