[Effects of domoic acid on membrane function of primary cultured rat glial cells].
OBJECTIVE
To study the effects of domoic acid (DA) on membrane function of primary cultured rat glial cell.


METHODS
After the glial cells were treated with 6.4 x 10(-2), 6.4 x 10(-3) and 6.4 x 10(-4) micromol/L DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, the membrane fluidity and the permeability were measured to reflect the membrane function.


RESULTS
After treatment of DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase were inhibited significantly, the membrane fluidity decreased and the membrane permeability increased. The fluorescence polarization and microviscosity in the low, middle and high dosage treatment groups were 0.0626 +/- 0.0051, 0.0685 +/- 0.0097, 0.0648 +/- 0.0086 and 0.3154 +/- 0.0298, 0.3510 +/- 0.0571, 0.3286 +/- 0.0504 respectively, compared with the control group (0.0481 +/- 0.0069 and 0.2338 +/- 0.0372) (P < 0.01).


CONCLUSIONS
DA has obvious effects on membrane function of rat glial cells and may cause further injury on the cells.
Liu
Lin-Lin
LL
Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Li
Long
L
Chen
Dan
D
Liu
Ying-Sheng
YS
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects; D009457:Neuroglia / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
C012301:domoic acid; D007608:Kainic Acid