Role of chitin binding domain of chitinase A of Streptomyces cyaneus SP-27 in protoplast formation from Schizophyllum commune.
Chitinase A (CHIA) of Streptomyces cyaneus SP-27 forms protoplasts from Schizophyllum commune mycelia when it is combined with alpha-1,3-glucanase of Bacillus circulans KA-304. An N-terminal chitin-binding domain truncated mutant (CatCHIA), which was expressed in Escherichia coli Rosetta-gami B (DE 3), lost most of its colloidal chitin- and powder chitin-binding activity. The colloidal chitin-hydrolyzing, the powder chitin-hydrolyzing, and the protoplast-forming activities of CatCHIA were lower than those of CHIA, suggesting that the chitin-binding domain contributes to the hydrolysis of chitin in the cell-wall of S. commune.
Yano
Shigekazu
S
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University, Kusatsu, Shiga, Japan. shige-y@sk.ritsumei.ac.jp
Honda
Arata
A
Rattanakit-Chandet
Nopakarn
N
Noda
Yuta
Y
Wakayama
Mamoru
M
Plikomol
Abhinya
A
Tachiki
Takashi
T
D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D009154:Mutation; D017434:Protein Structure, Tertiary; D011523:Protoplasts / Q000378:metabolism*; D012567:Schizophyllum / Q000166:cytology*; D013302:Streptomyces / Q000201:enzymology*
D002686:Chitin; D002688:Chitinases