title: Microleakage of human saliva in coronally unsealed obturated root canals in anaerobic conditions.
abstract: The purpose of this study was to determine the time required for anaerobic bacteria in natural human saliva to contaminate root-filled teeth. Thirty-two single-rooted teeth were cleaned, shaped, filled, and exposed to human saliva for 120 days. Teeth that had not leaked were subjected to polymerise chain reaction examination. Sixty-six percent of the experimental group were totally contaminated. A PCR examination revealed there was no contamination in the apical 3 mm of leakage-free teeth.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Iran.
authors.forename: K Ashofteh
authors.initials: KA
authors.lastname: Yazdi
authors.forename: S
authors.initials: S
authors.lastname: Bayat-Movahed
authors.forename: M
authors.initials: M
authors.lastname: Aligholi
authors.forename: S J
authors.initials: SJ
authors.lastname: Hayes
authors.forename: M H
authors.initials: MH
authors.lastname: Nekoofar
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000302:isolation & purification*; D001729:Bismuth / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000032:analysis; D003763:Dental Leakage / Q000145:classification / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D004587:Electrophoresis, Agar Gel; D004853:Epoxy Resins / Q000627:therapeutic use; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D016133:Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012389:Root Canal Obturation / Q000295:instrumentation / Q000379:methods*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D012463:Saliva / Q000382:microbiology*; D012834:Silver / Q000627:therapeutic use; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000627:therapeutic use; D019227:Tooth Apex / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D012834:Silver; C028044:epoxy resin AH-26; D006171:Gutta-Percha; D014025:Titanium; D012973:Sodium Hypochlorite; D001729:Bismuth