Changes in plasma membrane lipids, aquaporins and proton pump of broccoli roots, as an adaptation mechanism to salinity.
Salinity stress is known to modify the plasma membrane lipid and protein composition of plant cells. In this work, we determined the effects of salt stress on the lipid composition of broccoli root plasma membrane vesicles and investigated how these changes could affect water transport via aquaporins. Brassica oleracea L. var. Italica plants treated with different levels of NaCl (0, 40 or 80mM) showed significant differences in sterol and fatty acid levels. Salinity increased linoleic (18:2) and linolenic (18:3) acids and stigmasterol, but decreased palmitoleic (16:1) and oleic (18:1) acids and sitosterol. Also, the unsaturation index increased with salinity. Salinity increased the expression of aquaporins of the PIP1 and PIP2 subfamilies and the activity of the plasma membrane H(+)-ATPase. However, there was no effect of NaCl on water permeability (P(f)) values of root plasma membrane vesicles, as determined by stopped-flow light scattering. The counteracting changes in lipid composition and aquaporin expression observed in NaCl-treated plants could allow to maintain the membrane permeability to water and a higher H(+)-ATPase activity, thereby helping to reduce partially the Na(+) concentration in the cytoplasm of the cell while maintaining water uptake via cell-to-cell pathways. We propose that the modification of lipid composition could affect membrane stability and the abundance or activity of plasma membrane proteins such as aquaporins or H(+)-ATPase. This would provide a mechanism for controlling water permeability and for acclimation to salinity stress.
López-Pérez
Luis
L
Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, Campus Universitario de Espinardo, 30100 Murcia, Spain.
Martínez-Ballesta
María Del Carmen
Mdel C
Maurel
Christophe
C
Carvajal
Micaela
M
D000222:Adaptation, Physiological; D020346:Aquaporins / Q000378:metabolism* / Q000502:physiology; D001692:Biological Transport; D001937:Brassica / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism* / Q000502:physiology; D010539:Permeability; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006180:Proton-Translocating ATPases / Q000378:metabolism* / Q000502:physiology; D055049:Salt Tolerance / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
D020346:Aquaporins; D004573:Electrolytes; D008563:Membrane Lipids; D014867:Water; D012965:Sodium Chloride; D006180:Proton-Translocating ATPases