Gene expression profiling in anti-CD11d mAb-treated spinal cord-injured rats.
Acute administration of a mononclonal antibody (mAb) raised against the CD11d subunit of the leukocyte CD11d/CD18 integrin after spinal cord injury (SCI) in the rat greatly improves neurological outcomes. We have profiled gene expression in anti-CD11d and isotyped-matched control mAb-treated rats after SCI. Microarray analysis demonstrated reduced expression of pro-inflammatory cytokines and increased expression of inflammatory mediators that promote wound healing and the expression of scar proteins predicted to improve nerve growth. These changes in gene expression may reflect changes in the types of macrophages that populate the lesions in anti-CD11d mAb-treated rats.
Gris
Paul
P
The Spinal Cord Injury Team, BioTherapeutics Research Group, Robarts Research Institute, London, Canada.
Tighe
Allyson
A
Thawer
Sakina
S
Hemphill
Andrew
A
Oatway
Mark
M
Weaver
Lynne
L
Dekaban
Gregory A
GA
Brown
Arthur
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D018845:CD11 Antigens / Q000187:drug effects* / Q000378:metabolism; D002921:Cicatrix / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D016207:Cytokines / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000235:genetics* / Q000276:immunology; D014945:Wound Healing / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D018845:CD11 Antigens; D016207:Cytokines; D018836:Inflammation Mediators; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger