title: [Isolation, identification, and biodegradation characteristics of a quinoline-degrading bacterium].
abstract: A bacterial strain BW003, which could utilize quinoline as sole carbon, nitrogen and energy source, was isolated from the activated sludge in a coking wastewater treatment plant. According to the 16S rRNA gene sequence analysis, the strain was identified as Pseudomonas sp. Biodegradation experiments showed that the strain could degrade 192-911 mg/L of quinoline efficiently within 3-8 h, and the removal rates of quinoline were ranged from 96% to 98%. The optimum conditions for the degradation were 30 degrees C and pH 8. Metabolic products analysis revealed that at least 43% quinoline was first transformed into 2-hydroxyquinoline, 0.69% 2-hydroxyquinoline was then transformed into 2,8-dihydroxyquinoline, and subsequently into 8-hydroxycoumarin in the process of biodegradation. Additionally, at least 48% of nitrogen in quinoline was transformed into NH3-N directly and external carbon source could promote the nitrogen transformation, demonstrating that the quinoline and its metabolic products could be eliminated if controlling proper C/N ratio.
authors.affiliation: College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China. baiyh@pku.edu.cn
authors.forename: Yao-hui
authors.initials: YH
authors.lastname: Bai
authors.forename: Cui
authors.initials: C
authors.lastname: Zhao
authors.forename: Ya-na
authors.initials: YN
authors.lastname: Xiao
authors.forename: Dong-hui
authors.initials: DH
authors.lastname: Wen
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Tang
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011804:Quinolines / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D011804:Quinolines; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C037219:quinoline