title: Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: purification, characterization and potential application as a laundry detergent additive.
abstract: Two detergent stable alkaline serine-proteases (BM1 and BM2) from Bacillus mojavensis A21 were purified. The molecular weights of BM1 and BM2 enzymes determined by SDS-PAGE were approximately 29,00 Da and 15,50 Da, respectively. The optimum pH values of BM1 and BM2 proteases were shown to be 8.0-10.0 and 10.0, respectively. Both enzymes exhibited maximal activity at 60 degrees C, using casein as a substrate. The N-terminal amino acid sequences of BM1 and BM2 proteases were AQSVPYGISQIKA and AIPDQAATTLL, respectively. Both proteases showed high stability towards non-ionic surfactants. The enzymes were found to be relatively stable towards oxidizing agents. In addition, both enzymes showed excellent stability and compatibility with a wide range of commercial liquid and solid detergents. These properties and the high activity in high alkaline pH make these proteases an ideal choice for application in detergent formulations.
authors.affiliation: Laboratoire de Génie Enzymatique et de Microbiologie -Ecole Nationale d'Ingénieurs de Sfax, Sfax, Tunisia.
authors.forename: Anissa
authors.initials: A
authors.lastname: Haddar
authors.forename: Rym
authors.initials: R
authors.lastname: Agrebi
authors.forename: Ali
authors.initials: A
authors.lastname: Bougatef
authors.forename: Noomen
authors.initials: N
authors.lastname: Hmidet
authors.forename: Alya
authors.initials: A
authors.lastname: Sellami-Kamoun
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002364:Caseins / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D003902:Detergents / Q000737:chemistry* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016877:Oxidants / Q000737:chemistry; D012697:Serine Endopeptidases / Q000737:chemistry*; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D002364:Caseins; D003902:Detergents; D016877:Oxidants; C016680:polyacrylamide gels; D012697:Serine Endopeptidases