title: Comparison of the cytotoxic effect of lapachol, alpha-lapachone and pentacyclic 1,4-naphthoquinones on human leukemic cells.
abstract: The pentacyclic 1,4-naphthoquinones 1a-d were cytotoxic (IC(50) approximately 2-7 microM) to human leukemic cell lines K562 (oxidative stress-resistant), Lucena-1 (MDR phenotype) and Daudi. Fresh leukemic cells obtained from patients, some with the MDR phenotype, were also sensitive to these compounds. The pentacyclic 1,4-naphthoquinones 1a and 1c induced apoptotic cell death in cells from leukemic patients as determined by flow cytometry. Conversely, the cell lines were highly insensitive to lapachol (2) and alpha-lapachone (3). Mitomycin-C inhibited cell proliferation at concentrations as low as 0.5 microM. The low toxicity against lymphocytes activated by phytohemagglutinin shows that these compounds are selective for the cancer cells studied. Previous data suggest that these compounds (1a-d) can be bioactivated in situ by reduction followed by rearrangement leading to enones, which are powerful alkylating agents. In contrast, lapachol (2) and beta-lapachone (3), which cannot be bioactivated by reduction, showed little activity against the same cell lines.
authors.affiliation: Laboratório de Imunologia Tumoral, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Eduardo J S
authors.initials: EJ
authors.lastname: Salustiano
authors.forename: Chaquip D
authors.initials: CD
authors.lastname: Netto
authors.forename: Renata F
authors.initials: RF
authors.lastname: Fernandes
authors.forename: Alcides J M
authors.initials: AJ
authors.lastname: da Silva
authors.forename: Thiago S
authors.initials: TS
authors.lastname: Bacelar
authors.forename: Carolina P
authors.initials: CP
authors.lastname: Castro
authors.forename: Camilla D
authors.initials: CD
authors.lastname: Buarque
authors.forename: Raquel C
authors.initials: RC
authors.lastname: Maia
authors.forename: Vivian M
authors.initials: VM
authors.lastname: Rumjanek
authors.forename: Paulo R R
authors.initials: PR
authors.lastname: Costa
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D007938:Leukemia / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D016685:Mitomycin / Q000494:pharmacology; D009285:Naphthoquinones / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D009285:Naphthoquinones; D016685:Mitomycin; C008252:lapachol; C035342:1,4-naphthoquinone; C415043:alpha-lapachone