Establishment of vascular endothelial cell lines from the aortas of wild Japanese serows (Capricornis crispus).
Endothelial cell lines were established from the aortas of wild Japanese serows (Capricornis crispus) by transfection of a simian virus 40 (SV40) large T antigen gene. The cloned cell lines, designed SeET (Japanese serow endothelial-SV40T) cells, express SV40T antigen and retain cobblestone-like morphology. Although von Willbrand Factor (vWF) is expressed in the cells, the expression rate and the quantity are lower than in serow primary endothelial cells. The SeET cells exhibit positive uptake of acetylated low density lipoprotein and dose-dependent cell proliferation upon exposure to vascular endothelial growth factor. These results suggest that these SeET cells have preserved endothelial phenotypes and able to function with decreased expression of vWF. The SeET cell line will be a valuable tool for in vitro studies on the physiological properties of endothelial cells and for the propagation of viruses and parasites of Japanese serows.
Inoshima
Yasuo
Y
Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Japan. inoshima@gifu-u.ac.jp
Ishiguro
Naotaka
N
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism; D001011:Aorta / Q000166:cytology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006041:Goats / Q000033:anatomy & histology*; D006801:Humans; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming; D042461:Vascular Endothelial Growth Factor A