title: Mapping of RNA-protein interactions.
abstract: RNA-protein interactions are important biological events that perform multiple functions in all living organisms. The wide range of RNA interactions demands diverse conformations to provide contacts for the selective recognition of proteins. Various analytical procedures are presently available for quantitative analyses of RNA-protein complexes, but analytical-based mapping of these complexes is essential to probe specific interactions. In this overview, interactions of functional RNAs and RNA-aptamers with target proteins are discussed by means of mapping strategies.
authors.affiliation: Institute for Biological Resources and Functions & Center for Applied Near Field Optics Research (CAN-FOR), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba City 305-8562, Ibaraki, Japan.
authors.forename: Subash Chandra Bose
authors.initials: SC
authors.lastname: Gopinath
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D009711:Nucleotides / Q000037:antagonists & inhibitors / Q000737:chemistry; D010710:Phosphates / Q000037:antagonists & inhibitors / Q000737:chemistry; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D005609:Free Radicals; D009711:Nucleotides; D010710:Phosphates; D016601:RNA-Binding Proteins; D012313:RNA; D012260:Ribonucleases