title: Sensitive DNA biosensor based on a long-period grating formed on the side-polished fiber surface.
abstract: We demonstrate a sensitive DNA biosensor based on a long period grating (LPG) formed by a photolithograph process on the surface of a side-polished fiber. The biomolecules of the biosensor were immobilized on the silica surface between LPG patterns. The resonance wavelength was red-shifted after the binding of the poly-L-lysine, probe ssDNA and target ssDNA to the sensor surface. The overall wavelength shift after the successful DNA hybridization was 1.82 nm. The proposed LPG-based DNA biosensor is approximately 2.5 times more sensitive than the previously reported fiber grating-based DNA biosensors.
authors.affiliation: School of Information and Communication Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Jang
authors.forename: Kwang No
authors.initials: KN
authors.lastname: Park
authors.forename: Jun P
authors.initials: JP
authors.lastname: Kim
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
authors.forename: Oh J
authors.initials: OJ
authors.lastname: Kwon
authors.forename: Young-Geun
authors.initials: YG
authors.lastname: Han
authors.forename: Kyung Shik
authors.initials: KS
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004867:Equipment Design; D055100:Optical Fibers; D055620:Optical Phenomena; D010781:Photography; D011107:Polylysine; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D013499:Surface Properties
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D011107:Polylysine; D012822:Silicon Dioxide; D004247:DNA