title: [Effects of domoic acid on membrane function of primary cultured rat glial cells].
abstract: OBJECTIVE
  To study the effects of domoic acid (DA) on membrane function of primary cultured rat glial cell.
  
  
  METHODS
  After the glial cells were treated with 6.4 x 10(-2), 6.4 x 10(-3) and 6.4 x 10(-4) micromol/L DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase, the membrane fluidity and the permeability were measured to reflect the membrane function.
  
  
  RESULTS
  After treatment of DA for 24 h, the activities of Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase were inhibited significantly, the membrane fluidity decreased and the membrane permeability increased. The fluorescence polarization and microviscosity in the low, middle and high dosage treatment groups were 0.0626 +/- 0.0051, 0.0685 +/- 0.0097, 0.0648 +/- 0.0086 and 0.3154 +/- 0.0298, 0.3510 +/- 0.0571, 0.3286 +/- 0.0504 respectively, compared with the control group (0.0481 +/- 0.0069 and 0.2338 +/- 0.0372) (P < 0.01).
  
  
  CONCLUSIONS
  DA has obvious effects on membrane function of rat glial cells and may cause further injury on the cells.
authors.affiliation: Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Liu
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Ying-Sheng
authors.initials: YS
authors.lastname: Liu
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects; D009457:Neuroglia / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C012301:domoic acid; D007608:Kainic Acid