title: Hybrid of baculovirus and galactosylated PEI for efficient gene carrier.
abstract: Baculovirus, containing an appropriate eukaryotic promoter, is considered an attractive approach for an efficient and safe gene delivery vehicle. However, the drawbacks of baculovirus, such as the lack of specificity and the inactivation of baculovirus by the complement system in human serum, negatively affect efficient gene delivery. Therefore, a hybrid system utilizing the positive aspects of both viral and non-viral vector systems would be useful to overcome the obstacles of either system alone. In this study, we constructed a hybrid system composed of baculovirus (B) and galactosylated polyethylenimine (GP)/DNA complexes through electrostatic interaction. The resulting GP/B hybrid had suitable physicochemical properties and low cytotoxicity for use in gene therapy. Furthermore, the GP/B significantly enhanced transduction efficiency and showed good cell-specificity compared to either viral or non-viral vector systems. These results suggest that the GP/B hybrid system can be used in gene therapy to enhance transduction efficiency and hepatocyte specificity.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
authors.forename: You-Kyoung
authors.initials: YK
authors.lastname: Kim
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Hu-Lin
authors.initials: HL
authors.lastname: Jiang
authors.forename: Rohidas
authors.initials: R
authors.lastname: Arote
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Jere
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
mesh_terms: D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D048271:Chitosan / Q000031:analogs & derivatives* / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000378:metabolism; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D004337:Drug Carriers; C534328:galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine; D011094:Polyethyleneimine; D048271:Chitosan