Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment.
In this work, in vitro biosynthesis of silver nanoparticles was achieved using AgNO(3) as a substrate by Penicillium fellutanum isolated from coastal mangrove sediment. The biosynthesis was faster within minutes of silver ion coming in contact with the cell filtrate. Presence of silver nanoparticles in the culture filtrate was confirmed by absorption peak at 430 nm, as well under transmission electron microscope. The biosynthesis of nanoparticles was the maximum when the culture filtrate was treated with 1.0 mM AgNO(3), maintained at 0.3% NaCl and pH 6.0, incubated at 5 degrees C for 24h. The culture filtrate, precipitated with ammonium sulphate, was proved to have a single protein band with a molecular weight of 70 kDa using polyacrylamide gel electrophoresis. The present work highlighted the possibility of using the marine fungal strain of P. fellutanum to achieve a fast rate of nanoparticles synthesis.
Kathiresan
K
K
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India. kathirsum@rediffmail.com
Manivannan
S
S
Nabeel
M A
MA
Dhivya
B
B
D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000648:ultrastructure; D010407:Penicillium / Q000302:isolation & purification* / Q000378:metabolism*; D012834:Silver; D012835:Silver Nitrate / Q000378:metabolism
D012834:Silver; D012835:Silver Nitrate