title: Capillary zone electrophoresis with diode-array detection for analysis of local anaesthetics and opium alkaloids in urine samples.
abstract: The present study describes the simultaneous determination of four drugs, two local anaesthetics (lidocaine and bupivacaine) and two opium alkaloids (noscapine and papaverine) by capillary zone electrophoresis (CZE) with solid-phase extraction (SPE) procedure using Oasis HLB cartridges. Their recoveries ranged from 81 to 107% at the target concentrations of 2.0, 5.0 and 8.0 microgmL(-1) in spiked urine samples. Coefficients of variation of the recoveries ranged from 2.1 to 11.3% at these concentrations. The quantitation limits of the method were approximately 300 ngmL(-1) for the different compounds studied. The assay is very specific for these compounds and requires a short sample preparation procedure prior to the electrophoretic analysis.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, C/Fuentenueva s/n, University of Granada, E-18071 Granada, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Lombardo-Agüí
authors.forename: C
authors.initials: C
authors.lastname: Cruces-Blanco
authors.forename: A M
authors.initials: AM
authors.lastname: García-Campaña
mesh_terms: D000470:Alkaloids / Q000652:urine*; D000779:Anesthetics, Local / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009892:Opium / Q000652:urine*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000470:Alkaloids; D000779:Anesthetics, Local; D009892:Opium