title: Efficiency of methemoglobin, hemin and ferric citrate in catalyzing protein tyrosine nitration, protein oxidation and lipid peroxidation in a bovine serum albumin-liposome system: influence of pH.
abstract: Protein tyrosine nitration, protein oxidation and lipid peroxidation are nitrative/oxidative modification of protein and lipids. In this paper, a BSA (bovine serum albumin)-lecithin liposome system was used to study the nature of different forms of iron, including methemoglobin, hemin and ferric citrate, in catalyzing H(2)O(2)-nitrite system to oxidize protein and lipid as well as nitrate protein. It was found that in pH range of 5.0-9.0, in pure BSA solution or pure liposome solution, hemin and methemoglobin catalyzed protein tyrosine nitration and lipid peroxidation were decreased with the increasing of pH, while hemin and methemoglobin catalyzed protein oxidation was significantly and moderately increased, respectively. Lipid completely inhibited hemin catalyzed protein tyrosine nitration but only partially inhibited methemoglobin catalyzed protein tyrosine nitration, and its inhibitory effect on hemin induced protein oxidation was also more pronounced. In addition, BSA showed more efficient in inhibiting hemin and ferric citrate induced lipid peroxidation. At the same condition, ferric citrate was relatively ineffective in all tests. Considering protein tyrosine nitration, protein oxidation and lipid oxidation as overall oxidative damage, these results indicated that methemoglobin is more toxic than hemin and ferric citrate, the degradation procedure of heme containing macromolecules, e.g. hemoglobin to hemin and finally to low molecular weight bounded iron, is step by step detoxification. These results provide fundamental knowledge on oxidative/nitrative of biomolecules in lipid-protein coexistence system.
authors.affiliation: Quanzhou Normal University, PR China.
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Gao
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Song
authors.forename: Hailing
authors.initials: H
authors.lastname: Li
authors.forename: Zhonghong
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000818:Animals; D002384:Catalysis; D002417:Cattle; D005290:Ferric Compounds / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry*; D008706:Methemoglobin / Q000737:chemistry*; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D050050:Protein Carbonylation; D011506:Proteins / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D008081:Liposomes; D009566:Nitrates; D011506:Proteins; D012710:Serum Albumin, Bovine; C025314:ferric citrate; D006427:Hemin; D008706:Methemoglobin