title: Monitoring oxidative stress across worsening Child Pugh class of cirrhosis.
abstract: BACKGROUND
  Oxidative stress has been increasingly implicated in the pathogenesis and progression of cirrhosis.
  
  
  OBJECTIVE
  We studied oxidative stress in patients with cirrhosis by measuring markers reflecting pro-oxidant (serum malondialdehyde-MDA) and antioxidant factors (RBC catalase-CAT, superoxide dismutase-SOD and blood reduced glutathione-GSH) factors. The level of oxidative stress was also assessed with respect to functional compromise of liver, as determined by Child Turcotte Pugh (CTP) scoring.
  
  
  METHODS
  Case-controlled retrospective study.
  
  
  METHODS
  Twenty-three patients of cirrhosis along with 23 age and sex matched healthy controls were studied. Exclusion criteria were concurrent use of anti-oxidant drugs; co-existing diseases like DM, CKD; alcohol use, gastrointestinal bleed or blood transfusion within previous 2 weeks. Besides routine investigations, MDA, CAT, SOD and GSH levels were measured and compared with controls.
  
  
  METHODS
  Continuous variables were recorded as mean +/- SD; ANOVA-f test, followed by Tukey's test, was used to evaluate the significance of difference (P < 0.05) among groups.
  
  
  RESULTS
  Mean age of patients was 41.04 +/- 12.3 yrs. Patients showed a significant increase in MDA {control 3.31 +/- 0.25 (95% CI 3.21-3.41), Child B 6.30 +/- 0.4 (95% CI 6.03-6.53), Child C 8.05 +/- 0.66 (95% CI 7.29-8.81) nmol/l} and a significant decrease in levels of SOD {control 845.13 +/- 36.44 (95% CI 829.92-860.34), Child B 582.91 +/- 42.12 (95% CI 557.45-608.32), Child C 489.5 +/- 17.66 (95% CI 479.3-499.7) U/gm Hb}, CAT {controls 2.54 +/- 0.22 (95% CI 2.45-2.63), Child B 1.93 +/- 0.23 (95% CI 1.72-2.14), Child C 1.46 +/- 0.10 (95% CI 1.40-1.52) U/ gm Hb} and GSH {controls 6.52 +/- 0.25 (95% CI 6.42-6.52), Child B 3.85 +/- 0.18 (95%CI 3.74-3.96), Child C 2.99 +/- 0.30 (95% CI 2.82-3.16) mmol/ gm Hb}.
  
  
  CONCLUSIONS
  Oxidative stress is associated with the development and progression of cirrhosis.
authors.affiliation: Department of Medicine, University College of Medical Sciences and GTB Hospital, New Delhi-110 095, India. sumitbhandari06@gmail.com
authors.forename: Sumit
authors.initials: S
authors.lastname: Bhandari
authors.forename: Mukul P
authors.initials: MP
authors.lastname: Agarwal
authors.forename: S
authors.initials: S
authors.lastname: Dwivedi
authors.forename: B D
authors.initials: BD
authors.lastname: Banerjee
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D016001:Confidence Intervals; D018450:Disease Progression; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D008103:Liver Cirrhosis / Q000145:classification / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D008991:Monitoring, Physiologic; D018384:Oxidative Stress; D012189:Retrospective Studies; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione