[Investigation on degeneration of outer hair cells in guinea pig].
OBJECTIVE
To investigate the degeneration mechanics of outer hair cells in guinea pig.


METHODS
The mechanics of outer hair cells isolated by enzyme were observed under inverted microscope for 6-8 h continuously.


RESULTS
Over half of living outer hair cells could keep good conditions in 6 hours. During the degeneration there was always a longitudinal fold line from tip to base. Presence or absence of calcium, as well as lossing of stereociliary bundle, couldn't change the conditions of out hair cells.


CONCLUSIONS
Neither calcium nor stereociliary bundle is the decisive cause in keeping outer hair cells alive, and its degeneration may be basically related with something surrounding the cell.
Kang
Xixun
X
Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Kong
Weijia
W
Li
Wanrong
W
Zeng
Xiaorong
X
Zhnag
Song
S
Guo
Changkai
C
Huang
Xiang
X
D000818:Animals; D002118:Calcium; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology* / Q000473:pathology*; D003470:Culture Media; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology*
D003470:Culture Media; D002118:Calcium