Selectivity and antimicrobial action of bovine lactoferrin derived peptides against wine lactic acid bacteria.
In this study, the antibacterial activities of a bovine Lactoferrin pepsin hydrolysate (LFH) and a synthetic peptide derived from bovine lactoferricin (LfcinB(17-31)) have been evaluated against Oenococcus oeni and three additional lactic acid bacteria (LAB) known to cause spoilage during winemaking processes. Inhibition of bacterial growth was demonstrated in vitro in synthetic broth media (MRS) for both LFH and LfcinB(17-31). The bactericidal activity of the synthetic peptide was also assayed and found to vary depending on the bacterial species and the matrix in which exposure to peptide occurred (either MRS broth or white must). Specificity of LfcinB(17-31) for Lactobacillus brevis, Pediococcus damnosus, and O. oeni was demonstrated in must fermentation experiments in which these three LAB co-existed with the winemaking Saccharomyces cerevisiae T73 in the presence of the peptide. Finally, fermentation experiments also showed that LfcinB(17-31) at inhibitory concentrations did not alter either fermentation kinetics or specific enological parameters.
Enrique
María
M
Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas PO Box 73, 46100 Burjassot, Valencia, Spain.
Manzanares
Paloma
P
Yuste
María
M
Martínez
Mireia
M
Vallés
Salvador
S
Marcos
Jose F
JF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005285:Fermentation; D005506:Food Contamination / Q000517:prevention & control; D007700:Kinetics; D007777:Lactobacillaceae / Q000187:drug effects*; D007781:Lactoferrin / Q000494:pharmacology*; D007934:Leuconostoc / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010376:Pediococcus / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013045:Species Specificity; D014920:Wine / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D010446:Peptide Fragments; D007781:Lactoferrin