Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection.
Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n=7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of D- and L-Tyr derivatives were 2.9 and 2.2 micromol/L (S/N=3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution.
Bi
Weiwen
W
College of Chemistry, Sichuan University, Chengdu 610065, PR China.
Lei
Shaorong
S
Yang
Xiupei
X
Xu
Zemin
Z
Yuan
Hongyan
H
Xiao
Dan
D
Choi
Martin M F
MM
D000596:Amino Acids; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes; D008027:Light; D012996:Solutions; D013237:Stereoisomerism; D014443:Tyrosine / Q000302:isolation & purification*
D000596:Amino Acids; D005456:Fluorescent Dyes; D012996:Solutions; D014443:Tyrosine