Lipid hydroperoxides in human plasma after ethanol consumption.
Oxidative stress contributes to the pathogenesis of alcoholic liver disease. The purpose of this study is to estimate the amount of oxidative stress that is present when healthy humans consume moderate amounts of ethanol. Blood was collected from healthy volunteers before, 1 h, and 3 h after drinking 400 ml of Japanese rice wine at the rate of 100 ml per 5 min. The aldehyde dehydrogenase 2 genotype and the concentrations of blood ethanol, total lipid hydroperoxides (LOOH), and cholesterol hydroperoxides were determined. The plasma LOOH was found to have significantly increased 1h after drinking. Cholesterol hydroperoxides were not detected in plasma, either before or after drinking. There was no relationship between the LOOH and the ethanol concentration. We showed that one-shot of moderate ethanol consumption temporarily increases the plasma LOOH in healthy volunteers but excessive plasma LOOH compounds were eliminated within a short time.
Asano
Migiwa
M
Division of Legal Medicine, Department of Social Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. asano@med.kobe-u.ac.jp
Nushida
Hideyuki
H
Adachi
Junko
J
Nagasaki
Yasushi
Y
Nakagawa
Kanako
K
Kuse
Azumi
A
Ueno
Yasuhiro
Y
D000328:Adult; D000428:Alcohol Drinking / Q000097:blood*; D000444:Aldehyde Dehydrogenase / Q000235:genetics; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D002492:Central Nervous System Depressants / Q000097:blood; D002784:Cholesterol / Q000031:analogs & derivatives / Q000097:blood; D000431:Ethanol / Q000097:blood; D005260:Female; D053593:Forensic Toxicology; D005838:Genotype; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male
D002492:Central Nervous System Depressants; D008054:Lipid Peroxides; D000431:Ethanol; C002843:cholesterol hydroperoxide; D002784:Cholesterol; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial