title: Ratiometric and turn-on monitoring for heavy and transition metal ions in aqueous solution with a fluorescent peptide sensor.
abstract: A novel fluorescent peptide sensor containing tryptophan (donor) and dansyl fluorophore (acceptor) was synthesized for monitoring heavy and transition metal (HTM) ions on the basis of metal ion binding motif (Cys-X-X-X-Cys). The peptide probe successfully exhibited a turn on and ratiometric response for several heavy metal ions such as Hg(2+), Cd(2+), Pb(2+), Zn(2+), and Ag(+) in aqueous solution. The enhancements of emission intensity were achieved in the presence of the HTM ions by fluorescent resonance energy transfer (FRET) and chelation enhanced fluorescence (CHEF) effects. The detection limits of the sensor for Cd(2+), Pb(2+), Zn(2+), and Ag(+) were lower than the EPA's drinking water maximum contaminant levels (MCL). We described the fluorescent enhancement, binding affinity, and detection limit of the peptide probe for HTM ions.
authors.affiliation: Bioorganic Chemistry Laboratory, Department of Chemistry, Inha University, 253 Younghyun-Dong, Nam-Gu, Inchon-City, 402-751, South Korea.
authors.forename: Bishnu Prasad
authors.initials: BP
authors.lastname: Joshi
authors.forename: Junwon
authors.initials: J
authors.lastname: Park
authors.forename: Wan In
authors.initials: WI
authors.lastname: Lee
authors.forename: Keun-Hyeung
authors.initials: KH
authors.lastname: Lee
mesh_terms: D001665:Binding Sites; D002614:Chelating Agents; D003619:Dansyl Compounds; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D007477:Ions; D019216:Metals, Heavy / Q000032:analysis*; D015336:Molecular Probe Techniques; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D028561:Transition Elements / Q000032:analysis*; D014364:Tryptophan; D014867:Water
chemical_list: D002614:Chelating Agents; D003619:Dansyl Compounds; D007477:Ions; D019216:Metals, Heavy; D010455:Peptides; D012996:Solutions; D028561:Transition Elements; D014867:Water; D014364:Tryptophan