Radiation-induced matrix production of lung fibroblasts is regulated by interleukin-8.
OBJECTIVE
Lung fibrosis can be caused by radiation therapy during cancer treatment and therefore can be the limiting factor of the treatment. The factors that cause the actual fibrosis and the interaction between different cell types were investigated.


METHODS
Epithelial lung cells and fibroblasts were irradiated and different cytokines were measured in the supernatant. Also effects of radiation on the matrix production of fibroblasts were investigated.


RESULTS
Irradiation of isolated lung fibroblasts did not cause increased extracellular matrix production; however, the co-culturing of fibroblasts and irradiated lung epithelial cells or the treatment of fibroblasts with supernatants of irradiated epithelial cells did result in an increase. We were able to show that increased interleukin-8 (IL-8) levels led to increased matrix production.


CONCLUSIONS
IL-8 is not only a proinflammatory cytokine but it also stimulates collagen synthesis and matrix production and therefore could be a possible drug target in preventing radiation damage during cancer therapy.
Kuhlmann
Ulrike C
UC
Institute of Clinical and Molecular Immunology, Otto-von-Guericke-University-Magdeburg, Magdeburg.
Chwieralski
Caroline E
CE
Reinhold
Dirk
D
Welte
Tobias
T
Buhling
Frank
F
D045744:Cell Line, Tumor; D003094:Collagen / Q000096:biosynthesis; D016207:Cytokines / Q000032:analysis / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000528:radiation effects; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D016209:Interleukin-8 / Q000502:physiology*; D008168:Lung / Q000166:cytology
D016207:Cytokines; D016209:Interleukin-8; D003094:Collagen