[Effect of atorvastatin on lipopolysaccharide-induced expression of C-reactive protein in human pulmonary epithelial cells].
OBJECTIVE
To determine the effect of atorvastatin on lipopolysaccharide-induced expression of C-reactive protein in cultured A549 cells.


METHODS
A549 cells were incubated in DMEM medium containing lipopolysaccharide in the absence or presence of various concentrations of atorvastatin. After the incubation, the medium was collected and the level of C-reactive protein was measured by enzyme-linked immunosorbent assay. The cells were harvested and C-reactive protein mRNA was analyzed by reverse transcription polymerase chain reaction.


RESULTS
Incubation with lipopolysaccharide significantly induced a time and dose dependent increase in the mRNA expression and the production of C-reactive protein in A549 cells (P<0.05). Atorvastatin significantly decreased the lipopolysaccharide induced the mRNA expression and the production of C-reactive protein in a dose dependent manner in A549 cells (P<0.05).


CONCLUSIONS
Atorvastatin downregulates lipopolysaccharide-induced expression of C-reactive protein in cultured A549 cells, which may be its mechanism of anti-inflammation.
Wu
Shangjie
S
Department of Respiratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China. wu_shangjie@hotmail.com
Xing
Xiqian
X
Gan
Ye
Y
Zhao
Shuiping
S
Chen
Ping
P
D000069059:Atorvastatin; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D011758:Pyrroles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D006538:Heptanoic Acids; D008070:Lipopolysaccharides; D011758:Pyrroles; D012333:RNA, Messenger; D002097:C-Reactive Protein; D000069059:Atorvastatin