title: Expression of nestin mRNA is a differentiation marker in thyroid tumors.
abstract: Nestin is a maker that identifies stem cells in some adult tissues, and its expression is believed to relate to malignancy in cancer cells. In this study, we measured the expression levels of nestin mRNA in various kinds of thyroid tumor by the real-time quantitative reverse transcription-polymerase chain reaction. Unexpectedly, nestin mRNA was detected in almost all differentiated thyroid tumors and normal thyroid tissues, whereas extremely decreased expression was observed in anaplastic carcinomas, which are the most malignant of the thyroid follicular cell-derived tumors. These results suggest that nestin mRNA is a differentiation marker, and its expression does not relate to malignant characteristics in thyroid tumors.
authors.affiliation: Department of Laboratory Medicine, Osaka University Medical School, 2-2 D2, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yamada
authors.forename: Toru
authors.initials: T
authors.lastname: Takano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuzuka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Miya
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Mikio
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwatani
authors.forename: Akira
authors.initials: A
authors.lastname: Miyauchi
mesh_terms: D000236:Adenoma / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006042:Goiter / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D064231:Nestin; D009687:Nuclear Proteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000378:metabolism*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors