title: Manufacture of small calibre quadruple lamina vascular bypass grafts using a novel automated extrusion-phase-inversion method and nanocomposite polymer.
abstract: Long-term patency of expanded polytetrafluoroethylene (ePTFE) small calibre cardiovascular bypass prostheses (<6mm) is poor because of thrombosis and intimal hyperplasia due to low compliance, stimulating the search for elastic alternatives. Wall porosity allows effective post-implantation graft healing, encouraging endothelialisation and a measured fibrovascular response. We have developed a novel poly (carbonate) urethane-based nanocomposite polymer incorporating polyhedral oligomeric silsesquioxane (POSS) nanocages (UCL-NANO) which shows anti-thrombogenicity and biostability. We report an extrusion-phase-inversion technique for manufacturing uniform-walled porous conduits using UCL-NANO. Image analysis-aided wall measurement showed that two uniform wall-thicknesses could be specified. Different coagulant conditions revealed the importance of low-temperature phase-inversion for graft integrity. Although minor reduction of pore-size variation resulted from the addition of ethanol or N,N-dimethylacetamide, high concentrations of ethanol as coagulant did not provide uniform porosity throughout the wall. Tensile testing showed the grafts to be elastic with strength being directly proportional to weight. The ultimate strengths achieved were above those expected from haemodynamic conditions, with anisotropy due to the manufacturing process. Elemental analysis by energy-dispersive X-ray analysis did not show a regional variation of POSS on the lumen or outer surface. In conclusion, the automated vertical extrusion-phase-inversion device can reproducibly fabricate uniform-walled small calibre conduits from UCL-NANO. These elastic microporous grafts demonstrate favourable mechanical integrity for haemodynamic exposure and are currently undergoing in-vivo evaluation of durability and healing properties.
authors.affiliation: Centre for Nanotechnology, Biomaterials & Tissue Engineering, UCL Division of Surgery & Interventional Science, University College London, London, UK.
authors.forename: Sandip
authors.initials: S
authors.lastname: Sarkar
authors.forename: Gaetano
authors.initials: G
authors.lastname: Burriesci
authors.forename: Adam
authors.initials: A
authors.lastname: Wojcik
authors.forename: Nicholas
authors.initials: N
authors.lastname: Aresti
authors.forename: George
authors.initials: G
authors.lastname: Hamilton
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Seifalian
mesh_terms: D001026:Coronary Artery Bypass; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D011312:Pressure; D013718:Tensile Strength
chemical_list: D011108:Polymers