title: A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine.
abstract: For the ornithine fermentation pathway, described more than 70 years ago, genetic and biochemical information are still incomplete. We present here the experimental identification of the last four missing genes of this metabolic pathway. They encode L-ornithine racemase, (2R,4S)-2,4-diaminopentanoate dehydrogenase, and the two subunits of 2-amino-4-ketopentanoate thiolase. While described only for the Clostridiaceae to date, this pathway is shown to be more widespread.
authors.affiliation: CEA, DSV, IG, Genoscope, 2 rue Gaston Crémieux, Evry F-91057, France. fonk@genoscope.cns.fr
authors.forename: Nuria
authors.initials: N
authors.lastname: Fonknechten
authors.forename: Alain
authors.initials: A
authors.lastname: Perret
authors.forename: Nadia
authors.initials: N
authors.lastname: Perchat
authors.forename: Sabine
authors.initials: S
authors.lastname: Tricot
authors.forename: Christophe
authors.initials: C
authors.lastname: Lechaplais
authors.forename: David
authors.initials: D
authors.lastname: Vallenet
authors.forename: Carine
authors.initials: C
authors.lastname: Vergne
authors.forename: Anne
authors.initials: A
authors.lastname: Zaparucha
authors.forename: Denis
authors.initials: D
authors.lastname: Le Paslier
authors.forename: Jean
authors.initials: J
authors.lastname: Weissenbach
authors.forename: Marcel
authors.initials: M
authors.lastname: Salanoubat
mesh_terms: D000693:Anaerobiosis; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009952:Ornithine / Q000378:metabolism*; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D009952:Ornithine