[Rapid determination on cocoonase contents in maxilla of four silkmoth breeds by dot blotting].
OBJECTIVE
To determine the cocoonase content in maxilla of four silkmoth breeds of Bombyx mori and set up a dot blotting method to determine the content of the drug action components in Chinese medicines.


METHODS
The contents of cocoonase in maxilla of four silkworm breed were determined by dot blotting with rabbit antibody of anti-cocoonase as the first antibody. Blotting signals were analyzed by software of Quantity one.


RESULTS
Contents of cocoonase in maxilla of four silkmoth breeds were 6.41, 8.09, 7.62, and 1.88 microg/moth, respectively.


CONCLUSIONS
The difference among four silkmoth breeds was significant in the content of maxilla cocoonase. Dot blotting, as a convenient and accurate method could be used for determination of the contents of drug action components in Chinese crude drugs similar to silkworm.
Wang
Hou-Wei
HW
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. houwew@163.com
D000818:Animals; D000917:Antibody Formation; D012831:Bombyx / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism; D015151:Immunoblotting / Q000379:methods*; D008297:Male; D008419:Materia Medica / Q000737:chemistry*; D011786:Quality Control; D011817:Rabbits; D015203:Reproducibility of Results; D012697:Serine Endopeptidases / Q000276:immunology / Q000378:metabolism*
D004792:Enzyme Precursors; D008419:Materia Medica; D012697:Serine Endopeptidases