title: Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection.
abstract: We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
authors.affiliation: Institute of Imaging and Applied Optics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. jonas.kuehn@a3.epfl.ch
authors.forename: Jonas
authors.initials: J
authors.lastname: Kühn
authors.forename: Frédéric
authors.initials: F
authors.lastname: Montfort
authors.forename: Tristan
authors.initials: T
authors.lastname: Colomb
authors.forename: Benjamin
authors.initials: B
authors.lastname: Rappaz
authors.forename: Corinne
authors.initials: C
authors.lastname: Moratal
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pavillon
authors.forename: Pierre
authors.initials: P
authors.lastname: Marquet
authors.forename: Christian
authors.initials: C
authors.lastname: Depeursinge
mesh_terms: D002477:Cells / Q000648:ultrastructure*; D004867:Equipment Design; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D006696:Holography / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*