cDNA cloning and life-cycle stage-specific expression of coronin from Physarum polycephalum.
Coronin cDNA was cloned from the plasmodia of Physarum polycephalum. The amino acid sequence deduced from the cDNA was comprised of 449 residues and showed 60% identity to that of Dictyostelium discoideum coronin. Southern blot analysis suggested that the coronin gene present in the P. polycephalum genome might be a single copy. Coronin was expressed in diploid plasmodia, while it was not detected in haploid amoebae or spores.
Minami
Yoshiko
Y
Department of Biochemistry, Faculty of Science, Okayama University of Science, Okayama, Japan. minami@dbc.ous.ac.jp
Ishihara
Masaaki
M
Hayase
Masato
M
Sakaguchi
Tomohisa
T
Yubisui
Toshitsugu
T
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D008018:Life Cycle Stages / Q000235:genetics*; D008969:Molecular Sequence Data; D016839:Physarum polycephalum / Q000235:genetics* / Q000254:growth & development*; D016415:Sequence Alignment; D054378:Acetogenins
D015107:4-Butyrolactone; D018076:DNA, Complementary; C438920:coronin acetogenin; D054378:Acetogenins