Phase noise optimization in temporal phase-shifting digital holography with partial coherence light sources and its application in quantitative cell imaging.
In temporal phase-shifting-based digital holographic microscopy, high-resolution phase contrast imaging requires optimized conditions for hologram recording and phase retrieval. To optimize the phase resolution, for the example of a variable three-step algorithm, a theoretical analysis on statistical errors, digitalization errors, uncorrelated errors, and errors due to a misaligned temporal phase shift is carried out. In a second step the theoretically predicted results are compared to the measured phase noise obtained from comparative experimental investigations with several coherent and partially coherent light sources. Finally, the applicability for noise reduction is demonstrated by quantitative phase contrast imaging of pancreas tumor cells.
Remmersmann
Christian
C
Center for Biomedical Optics and Photonics, University of Münster,Robert-Koch-Strasse 45, 48129 Münster, Germany.
Stürwald
Stephan
S
Kemper
Björn
B
Langehanenberg
Patrik
P
von Bally
Gert
G
D000465:Algorithms; D016477:Artifacts; D002477:Cells / Q000166:cytology* / Q000473:pathology; D006696:Holography / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008029:Lighting; D008858:Microscopy, Phase-Contrast; D008962:Models, Theoretical; D055095:Optics and Photonics; D010190:Pancreatic Neoplasms / Q000473:pathology; D013997:Time Factors