title: Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.
abstract: Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 microM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.
authors.affiliation: Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Sungwoo
authors.initials: S
authors.lastname: Bae
authors.forename: Stefan
authors.initials: S
authors.lastname: Wuertz
mesh_terms: D001386:Azides / Q000378:metabolism / Q000494:pharmacology*; D001438:Bacteroidaceae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005456:Fluorescent Dyes / Q000378:metabolism / Q000494:pharmacology*; D050296:Microbial Viability; D016133:Polymerase Chain Reaction / Q000379:methods*; D011419:Propidium / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013170:Spores / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014871:Water Microbiology
chemical_list: D001386:Azides; D005456:Fluorescent Dyes; C533957:propidium monoazide; D011419:Propidium; D004247:DNA