title: [Individual identification and paternity testing of bulls using microsatellite].
abstract: The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
authors.affiliation: Lanzhou Vocational Technology College, Lanzhou, China. jwang_423@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Chou-Sheng
authors.initials: CS
authors.lastname: Liu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Fu-Qing
authors.initials: FQ
authors.lastname: Yu
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Zhang
authors.forename: Xiu-Zhu
authors.initials: XZ
authors.lastname: Song
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Wei
mesh_terms: D000818:Animals; D002417:Cattle; D033481:Chromosomes, Mammalian / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D014644:Genetic Variation / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics