title: [The influence of circadian clock gene on mouse sperm fertility in RNAi].
abstract: OBJECTIVE
  To study the influence of circadian Clock gene on the fertilization ability of sperm via attenuating the expression of Clock with RNAi in male mice.
  
  
  METHODS
  After the injection of RNAi plasmid into mice testes, the expression of circadian Clock gene of testis tissue was determined by western Blotting. The fertilization ability of sperm was evaluated by various indices, including litter size in mated female mice, sperm count, sperm motility, in vitro fertilization (IVF) rate and acrosome development.
  
  
  RESULTS
  The RNAi plasmid targeting circadian gene Clock attenuated the expression of CLOCK in mice testis, reduced in vitro fertilization CIVF) rate and the conception rate in viva, and also decreased sperm acrosin activity.
  
  
  CONCLUSIONS
  Circadian gene Clock is related to the reproductive function in male mice. It probably affect sperm fertilization ability by regulating sperm acrosin activity.
authors.affiliation: Department of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Jiang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Yu-hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Yan-you
authors.initials: YY
authors.lastname: Liu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Zheng-rong
authors.initials: ZR
authors.lastname: Wang
mesh_terms: D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics* / Q000378:metabolism; D005298:Fertility / Q000235:genetics*; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013075:Sperm Capacitation / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D056926:CLOCK Proteins; C538944:Clock protein, mouse