title: The putative tumor suppressor microRNA-101 modulates the cancer epigenome by repressing the polycomb group protein EZH2.
abstract: The Polycomb Repressive Complex 2 (PRC2) mediates epigenetic gene silencing by trimethylating histone H3 lysine 27 (H3K27me3) and is known to aberrantly silence tumor suppressor genes in cancer. EZH2, the catalytic subunit of PRC2, enhances tumorigenesis and is commonly overexpressed in several types of cancer. Our microRNA profiling of bladder transitional cell carcinoma (TCC) patient samples revealed that microRNA-101 (miR-101) is down-regulated in TCC, and we showed that miR-101 inhibits cell proliferation and colony formation in TCC cell lines. Furthermore, our results confirm that miR-101 directly represses EZH2 and stable EZH2 knockdowns in TCC cell lines create a similar growth suppressive phenotype. This suggests that abnormal down-regulation of miR-101 could lead to the overexpression of EZH2 frequently seen in cancer. We conclude that miR-101 may be a potent tumor suppressor by altering global chromatin structure through repression of EZH2.
authors.affiliation: Department of Urology, Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Friedman
authors.forename: Gangning
authors.initials: G
authors.lastname: Liang
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Liu
authors.forename: Erika M
authors.initials: EM
authors.lastname: Wolff
authors.forename: Yvonne C
authors.initials: YC
authors.lastname: Tsai
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.forename: Xianghong
authors.initials: X
authors.lastname: Zhou
authors.forename: Peter A
authors.initials: PA
authors.lastname: Jones
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics*; D000071221:Enhancer of Zeste Homolog 2 Protein; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics / Q000378:metabolism; D063151:Polycomb Repressive Complex 2; D014157:Transcription Factors / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors; D010089:Oxidoreductases, N-Demethylating; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2