Molecularly imprinted polymer for solid-phase extraction of ephedrine and analogs from human plasma.
A molecularly imprinted polymer (MIP) was synthesized and evaluated to selectively extract ephedrine from human plasma. The MIP synthesis was performed in chloroform with methacrylic acid as a functional monomer and the target alkaloid as a template molecule. The resulting MIP was applied to the selective extraction of ephedrine from a pure aqueous medium. A recovery about 74% was obtained using the MIP with only 7% on the nonimprinted polymer (NIP). A very straightforward selective SPE procedure was then successfully applied to the direct extraction of ephedrine from spiked human plasma with a high extraction recovery (68%) on the MIP with no recovery on the NIP. Moreover, the MIP was used for the selective extraction of catecholamine neurotransmitters, i.e. adrenaline and noradrenaline.
Lasáková
Martina
M
Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.
Thiébaut
Didier
D
Jandera
Pavel
P
Pichon
Valérie
V
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004809:Ephedrine / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
D011108:Polymers; D004809:Ephedrine