title: ES cell line establishment.
abstract: A method is described to establish mouse embryonic stem cell (ESC) lines from hybrid and inbred strains of mice. Attention is paid not only to the methodology for isolation and culture but also to the validation of freshly derived lines, in order to be maintained for prolonged time without significant differentiation or karyotype instability, and to provide reproducible germline transmission in chimaeric mice.
authors.affiliation: Department of Applied Genetics, ARTEMIS Pharmaceuticals, Cologne, Germany.
authors.forename: Heidrun
authors.initials: H
authors.lastname: Kern
authors.forename: Branko
authors.initials: B
authors.lastname: Zevnik
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D007621:Karyotyping; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy