title: Screening of SMG7-binding peptides by combination of phage display and docking simulation analysis.
abstract: We screened SMG7-binding peptides with phage display and docking simulation analysis. Although a consensus motif was absent in the phage display-derived candidates, we succeeded to find a peptide CDDRPPKSC, which can bind specifically to SMG7. We conclude that docking simulation helps to find high-affinity peptides efficiently, even if the phage display-screened candidates lack a consensus region.
authors.affiliation: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Inohana, Chiba 260-8670, Japan.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Zahed
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Akiko
authors.initials: A
authors.lastname: Suganami
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Harada
authors.forename: Masaki
authors.initials: M
authors.lastname: Takiguchi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D003198:Computer Simulation; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002352:Carrier Proteins; D019151:Peptide Library; D010455:Peptides; C494578:SMG7 protein, human