Enhancement of lanthanum (III) on sodium currents in acutely isolated hippocampal CA1 neurons of rat.
The effects of lanthanum (III) (La(3+)) on voltage-gated sodium channel currents (I (Na)) in freshly dissociated rat hippocampal CA1 neurons were studied using the whole-cell patch clamp techniques. La(3+) reversibly enhanced I (Na) in a concentration- and voltage-dependent manner. The 50% enhancement concentration (EC(50)) of La(3+) on I (Na) was 9.93 microM. In addition, 10 microM La(3+) shifted the steady state activation curve of I (Na) towards positive potential and the steady state inactivation curve towards negative potential without changing the slope factor. These results indicated that La(3+) could increase the amplitudes of I (Na) and change the activation and inactivation courses of I (Na) even in very low concentration.
Du
Huizhi
H
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, PR China. duhuizhi@sxu.edu.cn
Yang
Pin
P
D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007811:Lanthanum / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology
D015222:Sodium Channels; D007811:Lanthanum