[Semen descurainiae inhibits CYP11B1, CYP11B2 and TGF-beta1 mRNA expression in left ventricular].
OBJECTIVE
To investigate the effects of Semen descurainiae and Captopril on CYP11B1, CYP11B2 and TGF-beta1 mRNA expression of heart tissue in rats treated with Abdominal Aortic Banding.


METHODS
Ventricular remodeling was induced by abdominal aortic banding (AAB) in rats. After 30 days' treatment, the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; Then the CYP11B, CYP11B2 and TGF-beta1 mRNA expression of left ventricle were detected by Real-time PCR, respectively.


RESULTS
The experimental data demonstrated that Semen descurainiae decreased the indexes of LVW/BW and HW/BW, down-regulated CYP11B, CYP11B2 and TGF-beta1 mRNA expression in left ventricle (P<0.05).


CONCLUSIONS
Semen desceurainiae can significantly inhibit the experimental ventricular remodeling; the mechanism is related to its ability to attenuate the mRNA expression of CYP11B1, CYP11B2 and TGF-beta1 in left ventricle. The inhibition of aldosterone key gene expression by Semen descurainiae may contribute to its effect on restraint cardiac remodeling.
Guo
Juan
J
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. guojuan338@yahoo.com.cn
Chen
Chang-Xun
CX
Du
Jun
J
Gu
Wei-Liang
WL
Shen
Yun-Hui
YH
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D019607:Brassicaceae / Q000737:chemistry*; D002312:Cardiomyopathy, Hypertrophic / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase