Hematopoietic stem cell transplantation without irradiation.
Hematopoietic stem cell (HSC) transplantation is limited by histocompatibility barriers and by lack of space in bone marrow niches. These obstacles prevent in vivo analysis of histoincompatible mutant stem cells and of HSC functions in non-irradiated mice. By genetically combining immunodeficiency with impaired function of the growth factor receptor Kit in mice, we generated a 'universal' HSC recipient that efficiently accepts long-term histocompatible and histoincompatible HSCs without prior irradiation.
Waskow
Claudia
C
Center for Regenerative Therapies Dresden, Dresden University of Technology, Germany. claudia.waskow@crt-dresden.de
Madan
Vikas
V
Bartels
Susanne
S
Costa
Céline
C
Blasig
Rosel
R
Rodewald
Hans-Reimer
HR
D000818:Animals; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000276:immunology*; D014916:Whole-Body Irradiation
D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; D019009:Proto-Oncogene Proteins c-kit