title: Preliminary crystallographic study of two cuticle-degrading proteases from the nematophagous fungi Lecanicillium psalliotae and Paecilomyces lilacinus.
abstract: Cuticle-degrading proteases are extracellular subtilisin-like serine proteases that are secreted by entomopathogenic and nematophagous fungi. These proteases can digest the host cuticle during invasion of an insect or nematode and serve as a group of important virulence factors during the infection of nematodes by nematophagous fungi. To elucidate the mechanism of interaction between the proteases and the nematode cuticle, two cuticle-degrading proteases, Ver112 from Lecanicillium psalliotae (syn. Verticillium psalliotae) and PL646 from Paecilomyces lilacinus, were studied. The Ver112 protein and the complex between PL646 and the substrate-like tetrapeptide inhibitor methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (MSU-AAPV) were crystallized using the hanging-drop vapour-diffusion method at 289 K. The crystals were analyzed by X-ray diffraction to resolutions of 1.65 and 2.2 A, respectively. These analyses identified that crystals of Ver112 belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 43.7, b = 67.8, c = 76.3 A, alpha = beta = gamma = 90 degrees . In contrast, crystals of the PL646-MSU-AAPV complex belonged to space group P2(1), with unit-cell parameters a = 65.1, b = 62.5, c = 67.6 A, beta = 92.8 degrees .
authors.affiliation: Yunnan University, Kunming, People's Republic of China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Ye
authors.forename: Lianming
authors.initials: L
authors.lastname: Liang
authors.forename: Qili
authors.initials: Q
authors.lastname: Mi
authors.forename: Jinkui
authors.initials: J
authors.lastname: Yang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
authors.forename: Yuna
authors.initials: Y
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Meng
authors.forename: Keqin
authors.initials: K
authors.lastname: Zhang
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D034582:Integumentary System; D010143:Paecilomyces / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010447:Peptide Hydrolases