title: Quantitative phosphoproteomics reveals a cluster of tyrosine kinases that mediates SRC invasive activity in advanced colon carcinoma cells.
abstract: The nonreceptor tyrosine kinase Src is frequently overexpressed and/or activated in human colorectal carcinoma (CRC), and its increased activity has been associated with a poor clinical outcome. Src has been implicated in growth and invasion of these cancer cells by still not well-known mechanisms. Here, we addressed Src oncogenic signaling using quantitative phosphoproteomics. Src overexpression increased growth and invasiveness of metastatic SW620 CRC cells. Stable isotope labeling with amino acids in cell culture in combination with liquid chromatography tandem mass spectrometry allowed the identification of 136 proteins which exhibited a significant increase in and/or association with tyrosine phosphorylation upon Src expression. These mainly include signaling, cytoskeleton, and vesicular-associated proteins. Interestingly, Src also phosphorylated a cluster of tyrosine kinases, i.e., the receptors Met and EphA2, the cytoplasmic tyrosine kinase Fak, and pseudo-tyrosine kinase SgK223, which were required for its invasive activity. Similar results were obtained with metastatic Colo205 CRC cells that exhibit high endogenous Src activity. We concluded that Src uses a tyrosine kinases network to promote its invasive activity in CRC and this implicates a reverse signaling via tyrosine kinase receptors. Targeting these tyrosine kinases may be of significant therapeutic value in this cancer.
authors.affiliation: Centre National de la Recherche Scientifique UMR5237, University of Montpellier 1 and 2, CRBM, Montpellier, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Leroy
authors.forename: Camille
authors.initials: C
authors.lastname: Fialin
authors.forename: Audrey
authors.initials: A
authors.lastname: Sirvent
authors.forename: Valérie
authors.initials: V
authors.lastname: Simon
authors.forename: Serge
authors.initials: S
authors.lastname: Urbach
authors.forename: Joël
authors.initials: J
authors.lastname: Poncet
authors.forename: Bruno
authors.initials: B
authors.lastname: Robert
authors.forename: Patrick
authors.initials: P
authors.lastname: Jouin
authors.forename: Serge
authors.initials: S
authors.lastname: Roche
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D014183:Transplantation, Heterologous; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D011505:Protein-Tyrosine Kinases; D019061:src-Family Kinases