title: Hepatitis C virus genotype in blood donors and associated liver disease in Indonesia.
abstract: OBJECTIVE
  The aim of this study was to investigate the distribution of hepatitis C virus (HCV) genotype and the possible association between genotype and HCV-associated liver disease in Indonesia.
  
  
  METHODS
  32 anti-HCV-positive asymptomatic carriers (AC), 55 chronic hepatitis (CH), 41 liver cirrhosis (LC), and 35 hepatocellular carcinoma (HCC) patients were included in this study. HCV genotyping was performed by phylogenetic analysis of the NS5B and 5'-UTR regions.
  
  
  RESULTS
  The HCV subtype 1b (36.5%), based on NS5B region, was the most prevalent, followed by subtypes 3k (15.4%), 2a (14.4%), 1a (12.5%) and 1c (12.5%), and 2e (4.8%). Subtypes 2f, 3a, 3b, and 4a were also found in some of the samples. HCV subtypes 3k (40.0%) and 1a (35.0%) were the two major subtypes in AC. HCV subtype 1b was not found in AC, but it was common in CH (31.3%), LC (50.0%), and HCC (57.1%).
  
  
  CONCLUSIONS
  HCV subtype 1b was prevalent in samples of HCV-associated liver disease patients, including CH, LC and HCC. The percentage of subtype 1b was increased with the disease severity (AC < CH < LC < HCC).
authors.affiliation: Molecular Epidemiology Division, Mochtar Riady Institute for Nanotechnology, Lippo Karawaci, Tangerang, Indonesia. autama@mrinstitute.org
authors.forename: Andi
authors.initials: A
authors.lastname: Utama
authors.forename: Bugi Ratno
authors.initials: BR
authors.lastname: Budiarto
authors.forename: Dewi
authors.initials: D
authors.lastname: Monasari
authors.forename: Theresia Imelda
authors.initials: TI
authors.lastname: Octavia
authors.forename: Ivan Stevanus
authors.initials: IS
authors.lastname: Chandra
authors.forename: Rino Alvani
authors.initials: RA
authors.lastname: Gani
authors.forename: Irsan
authors.initials: I
authors.lastname: Hasan
authors.forename: Andri
authors.initials: A
authors.lastname: Sanityoso
authors.forename: Upik Anderiani
authors.initials: UA
authors.lastname: Miskad
authors.forename: Irawan
authors.initials: I
authors.lastname: Yusuf
authors.forename: Laurentius Adrianus
authors.initials: LA
authors.lastname: Lesmana
authors.forename: Ali
authors.initials: A
authors.lastname: Sulaiman
authors.forename: Susan
authors.initials: S
authors.lastname: Tai
mesh_terms: D001782:Blood Donors; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D007214:Indonesia; D008107:Liver Diseases / Q000175:diagnosis / Q000821:virology*; D010802:Phylogeny; D017361:Viral Nonstructural Proteins / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase
chemical_list: D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase