title: Palladium(II) complexes with R2edda-derived ligands. Part II. Synthesis, characterization and in vitro antitumoral studies of R2eddip esters and palladium(II) complexes.
abstract: New R(2)eddip-type esters (R = cyclopentyl, L3 x 2 HCl 1.5 H(2)O; cyclohexyl, L4 x 2 HCl x H(2)O) and corresponding palladium(II) complexes, [PdCl(2)L3] (3) and [PdCl(2)L4] x H(2)O (4), as well as [PdCl(2)L2] (2; L2 diisobutyl ester of eddip) were synthesized and characterized by IR, (1)H and (13)C NMR spectroscopies and elemental analysis. The crystal structure of L3 x 2 HCl x 2 CHCl(3) was resolved and is given herein. The NMR spectroscopy confirmed the presence of two isomers (from three possible) for each palladium(II) complex. DFT calculations support the formation of two diastereoisomers. In addition, antitumoral investigations were performed and these investigations also included the diisopropyl ester of eddip (L1) and corresponding palladium(II) complex, [PdCl(2)L1] (1). In vitro antiproliferative activity was determined against several tumor cell lines HeLa, Fem-x, K562 and rested and stimulated normal immunocompetent cells (human peripheral blood mononuclear cells--PBMCs) using MTT test.
authors.affiliation: Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski Trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Bojana B
authors.initials: BB
authors.lastname: Zmejkovski
authors.forename: Goran N
authors.initials: GN
authors.lastname: Kaluderović
authors.forename: Santiago
authors.initials: S
authors.lastname: Gómez-Ruiz
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Zizak
authors.forename: Dirk
authors.initials: D
authors.lastname: Steinborn
authors.forename: Harry
authors.initials: H
authors.lastname: Schmidt
authors.forename: Reinhard
authors.initials: R
authors.lastname: Paschke
authors.forename: Zorica D
authors.initials: ZD
authors.lastname: Juranić
authors.forename: Tibor J
authors.initials: TJ
authors.lastname: Sabo
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007536:Isomerism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D010165:Palladium / Q000737:chemistry* / Q000494:pharmacology*; D011789:Quantum Theory
chemical_list: D000970:Antineoplastic Agents; D008024:Ligands; D010165:Palladium