title: Essential oil composition and internal transcribed spacer (ITS) sequence variability of four South-Croatian Satureja species (Lamiaceae).
abstract: The purpose of this study was to compare the essential oil profiles of four South-Croatian Satureja species, as determined by GC/FID and GC/MS, with their DNA sequences for an internal transcribed spacer (ITS1-5.8S-ITS2) of the nuclear ribosomal DNA. A phylogenetic analysis showed that S. montana and S. cuneifolia, characterized by a similar essential oil composition, rich in the monoterpene hydrocarbon carvacrol, clustered together with high and moderate bootstrap support. On the contrary, S. subspicata and S. visianii, characterized by quite unique essential oil compositions, clustered together with the moderate bootstrap support. All four Croatian Satureja species clustered in one clade, separately from Macaronesian S. hortensis,although it had essential oil composition similar to that of S. montana and S. cuneifolia. This is the first report on the comparison between the phytochemical and DNA sequence data in Satureja species and useful contribution to the better understanding of interspecies relationships in this genus.
authors.affiliation: University of Split, Faculty of Science, Department of Biology, Teslina 12, 21000 Split, Croatia.
authors.forename: Nada
authors.initials: N
authors.lastname: Bezić
authors.forename: Ivica
authors.initials: I
authors.lastname: Samanić
authors.forename: Valerija
authors.initials: V
authors.lastname: Dunkić
authors.forename: Visnja
authors.initials: V
authors.lastname: Besendorfer
authors.forename: Jasna
authors.initials: J
authors.lastname: Puizina
mesh_terms: D001483:Base Sequence; D017523:Croatia; D000081007:Cymenes; D004275:DNA, Ribosomal / Q000235:genetics*; D039821:Monoterpenes; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry*; D010802:Phylogeny; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry*; D031362:Satureja / Q000737:chemistry* / Q000235:genetics; D013045:Species Specificity
chemical_list: D000081007:Cymenes; D004275:DNA, Ribosomal; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; C073316:carvacrol