title: Detection of cerebral metabolites in a canine model of ischemic stroke using 1H magnetic resonance spectroscopy.
abstract: Proton magnetic resonance spectroscopy ((1)H MRS) provides in vivo biochemical information on tissue metabolites. The purpose of this study was to investigate the serial metabolic changes of (1)H MRS in the cerebrum of ischemic dogs. An ischemic stroke was induced in five health laboratory beagle dogs by permanent middle cerebral artery occlusion using a silicone plug. (1)H MRS was serially performed three times with a 1.5-T MR system: before, three days after and 10days after the stroke. Immunohistochemical staining was performed to determine the expression of neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) at both the ipsilateral and contralateral cerebral cortex. Reduced levels of N-acetyl-asparate (p<0.05), choline (Cho), creatine (Cr) and myo-inositol (mI), and a marked increase in the lactate (Lac) level (p<0.01) were found at three days after the stroke. At 10days after the stroke, the levels of Lac significantly increased (p<0.01); however, the other metabolites were partially elevated. The changes of Cr, Cho and mI were not statistically significant (p>0.05) when the before and after stroke values were compared. There was a significant loss of NeuN and GFAP immunoreactivity at the ischemic core. (1)H MRS may be to a useful diagnostic tool for the evaluation of ischemic stroke in dogs.
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, #1 Hwayang-dong, Gwang-jin-gu, Seoul 143-701, South Korea.
authors.forename: Byeong-Teck
authors.initials: BT
authors.lastname: Kang
authors.forename: Dong-Pyo
authors.initials: DP
authors.lastname: Jang
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong-In
authors.initials: DI
authors.lastname: Jung
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Gu
authors.forename: Chae-Young
authors.initials: CY
authors.lastname: Lim
authors.forename: Young-Bo
authors.initials: YB
authors.lastname: Kim
authors.forename: Fu-Shi
authors.initials: FS
authors.lastname: Quan
authors.forename: Hyung-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Eung-Je
authors.initials: EJ
authors.lastname: Woo
authors.forename: Zang-Hee
authors.initials: ZH
authors.lastname: Cho
authors.forename: Hee-Myung
authors.initials: HM
authors.lastname: Park
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000662:veterinary*; D002371:Cat Diseases / Q000473:pathology; D002415:Cats; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology / Q000662:veterinary; D008279:Magnetic Resonance Imaging / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D020521:Stroke / Q000473:pathology / Q000662:veterinary*