title: T allele of -344 C/T polymorphism in aldosterone synthase gene is not associated with resistant hypertension.
abstract: Resistant hypertension (RH) is the maintenance of elevated blood pressure concurrent with the use of three different anti-hypertensive drugs, one of which is a diuretic. The Renin-Angiotensin-Aldosterone System plays a major role in volume-dependent hypertension. Therefore, its components are interesting targets for genetic association studies. This work focused on the -344 C/T polymorphism in the CYP11b2 gene, which encodes aldosterone synthase. This work evaluates the association between T allele and resistance to anti-hypertensive treatment. Genotyping analysis included 88 subjects with RH, 142 who were responsive to anti-hypertensive treatment and 110 subjects as a control group. Plasmatic concentrations of aldosterone, renin and cortisol, carotid intima-media thickness and carotid-femoral pulse wave velocity were assessed in a smaller subset of hypertensive patients. An association was found between T allele and hypertension (P<0.005), but there was no difference in allele frequencies between both hypertensive groups. There was no difference in plasmatic parameters either, in remodeling indicators between the genotypic groups.
authors.affiliation: Cardiovascular Pharmacology Laboratory, Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. lacchi_4@terra.com.br
authors.forename: Riccardo
authors.initials: R
authors.lastname: Lacchini
authors.forename: Maricene
authors.initials: M
authors.lastname: Sabha
authors.forename: Fernanda B
authors.initials: FB
authors.lastname: Coeli
authors.forename: Fabrício F
authors.initials: FF
authors.lastname: Favero
authors.forename: Juan
authors.initials: J
authors.lastname: Yugar-Toledo
authors.forename: Tatiane C
authors.initials: TC
authors.lastname: Izidoro-Toledo
authors.forename: Valéria C
authors.initials: VC
authors.lastname: Sandrim
authors.forename: José E
authors.initials: JE
authors.lastname: Tanus-Santos
authors.forename: Maricilda P
authors.initials: MP
authors.lastname: de Mello
authors.forename: Heitor
authors.initials: H
authors.lastname: Moreno
mesh_terms: D000328:Adult; D000450:Aldosterone / Q000097:blood; D000483:Alleles; D001794:Blood Pressure / Q000502:physiology; D002339:Carotid Arteries / Q000000981:diagnostic imaging; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D004351:Drug Resistance; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D006973:Hypertension / Q000188:drug therapy / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000502:physiology; D012150:Polymorphism, Restriction Fragment Length; D012083:Renin / Q000097:blood; D014463:Ultrasonography
chemical_list: D000450:Aldosterone; D019405:Cytochrome P-450 CYP11B2; D012083:Renin; D006854:Hydrocortisone