In vitro adhesion of human dermal fibroblasts on iron cross-linked alginate films.
We evaluated the potential of alginate film incorporating ferric ions as a gelling agent (Fe-alginate) in comparison with that incorporating calcium ions (Ca-alginate) as a scaffold for culturing normal human dermal fibroblasts (NHDF). NHDF adhered to Fe-alginate and proliferated well, but no growth of the cells was observed on Ca-alginate. Since vitronectin and fibronectin play pivotal roles in cellular adhesion, their participation in NHDF behavior on alginate surfaces was investigated. We found that vitronectin was a critical element for initial attachment and spreading of NHDF on Fe-alginate. The surface properties of both alginate films were characterized in terms of protein adsorption ability and surface wettability, and it was revealed that Fe-alginate film adsorbed a significantly higher amount of proteins, including vitronectin and fibronectin, and had a higher surface hydrophobicity than Ca-alginate film. Moreover, under serum-free conditions, only a small number of NHDF were able to attach to the surface of Fe-alginate. Fe-alginate appeared to provide an appropriate surface for cellular attachment by adsorption of serum proteins such as vitronectin. These results suggest that Fe-alginate can serve as a scaffold for human fibroblasts and may be useful for tissue engineering research and other biomedical applications.
Machida-Sano
Ikuko
I
Department of Biology, School of Education, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. ikuko@ruri.waseda.jp
Matsuda
Yasushi
Y
Namiki
Hideo
H
D000327:Adsorption; D000464:Alginates / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry; D018929:Cell Culture Techniques; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology*; D005353:Fibronectins / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007477:Ions; D007501:Iron / Q000737:chemistry*; D012867:Skin / Q000166:cytology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D019096:Vitronectin / Q000737:chemistry
D000464:Alginates; D001798:Blood Proteins; D003432:Cross-Linking Reagents; D003470:Culture Media; D005353:Fibronectins; D006603:Hexuronic Acids; D007477:Ions; D019096:Vitronectin; D020723:Glucuronic Acid; D007501:Iron