title: Effect of Quyu Jiedu granule on microenvironment of ova in patients with endometriosis.
abstract: OBJECTIVE
  To observe the effect of Quyu Jiedu Granules (, QJG) on the micro- microenvironment of ova in patients with endometriosis (EM). environment
  
  
  METHODS
  Twenty EM patients who received in vitro fertilization and embryo transfer (IVF-ET) were randomized equally into a treated group and a control group. Further, 20 patients who received IVF-ET due to oviduct factors were enrolled into a non-endometriosis group. The dosage of gonadotrophic hormone used, the number of ova attained, fertilization rate and clinical pregnancy rate were all observed, and the levels of tumor necrosis factor alpha (TNF-right harpoon over left harpoon) and interleukin 6 (IL-6) in follicular fluid as well as their mRNA expressions in ovarian granular cells were detected by RT-PCR on the very day of ovum attainment.
  
  
  RESULTS
  The ova attainment (13.80+/-6.87) and fertilization rate (0.69+/-0.31) in the treated group were all higher than the corresponding values in the control group (9.80+/-5.32 and 0.47+/-0.22); the follicular fluid contents of TNF-alpha and IL-6 in the treated group were 1.38+/-0.21 ng/mL and 130.56+/-12.81 pg/mL, respectively, which were lower than those in the control group (1.98+/-0.34 ng/mL and 146.83+/-17.65 pg/mL, respectively). Further, the treated group showed much lower mRNA expressions of TNF-alpha and IL-6 in ovarian granular cells.
  
  
  CONCLUSIONS
  The elevation of TNF-alpha and IL-6 contents in follicular fluid and their mRNA expressions in ovarian granular cells are possibly related to the low quality of ova in EM; QJG might raise the ova quality by reducing TNF-alpha and IL-6 levels to improve the living micro-environment for the ova.
authors.affiliation: Reproductive and Genetic Center of Intrgrative Medivine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, China. f_lian@163.com
authors.forename: Fang
authors.initials: F
authors.lastname: Lian
authors.forename: Xin-Ling
authors.initials: XL
authors.lastname: Li
authors.forename: Zhen-Gao
authors.initials: ZG
authors.lastname: Sun
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhang
authors.forename: Yan-He
authors.initials: YH
authors.lastname: Liu
authors.forename: Feng-Mei
authors.initials: FM
authors.lastname: Ma
mesh_terms: D000328:Adult; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D004715:Endometriosis / Q000188:drug therapy*; D005260:Female; D015571:Follicular Fluid / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D010060:Ovulation / Q000187:drug effects; D010063:Ovum / Q000187:drug effects* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C544738:quyu jiedu