title: Genetic variant of the p22PHOX component of NADPH oxidase C242T and the incidence of gastric cancer in Japan.
abstract: OBJECTIVE
  Reactive oxygen species has been implicated in the development of gastric cancer. NADPH oxidase, a major source of superoxide generation play a critical role in H. pylori related gastric inflammation. We aimed to clarify the effect of C242T polymorphism of p22PHOX, an essential component of NADPH oxidase on the risk of gastric cancer, gastric ulcer and duodenal ulcer in a Japanese population.
  
  
  METHODS
  376 gastric cancer patients and 436 cancer-free subjects participated in this study. The p22PHOX C242T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  In the non-cancer subjects, the p22PHOX genotype distribution was 365CC (83.7%), 63CT (15.4%), and 4TT (0.9%). Meanwhile, the p22PHOX genotype distribution in gastric cancer was 311CC (82.7%), 63CT (16.8%), and 2TT (0.5%). There was no significant difference between two groups in the distribution of T carrier frequency (OR=1.04, 95% CI=0.71-1.53). No association was also observed between p22PHOX genotypes and Lauren's classification, tumor stage and anatomical locations.
  
  
  CONCLUSIONS
  It appears that the C242T polymorphism of p22PHOX is not associated with the incidence of gastric cancer in the Japanese population.
authors.affiliation: Department of Gastroenterology, Fujita Health University School of Medicine, Japan. tomomiccyu@yahoo.co.jp
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Tahara
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Fangyu
authors.initials: F
authors.lastname: Wang
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yoshioka
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okubo
authors.forename: Naoko
authors.initials: N
authors.lastname: Maruyama
authors.forename: Tashiaki
authors.initials: T
authors.lastname: Kamano
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujita
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nagasaka
authors.forename: Masami
authors.initials: M
authors.lastname: Iwata
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takahama
authors.forename: Makoto
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakano
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirata
mesh_terms: D000368:Aged; D000483:Alleles; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics*; D009367:Neoplasm Staging; D012150:Polymorphism, Restriction Fragment Length; D016036:Seroepidemiologic Studies; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics* / Q000473:pathology
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human