title: Pulmonary expression of nitric oxide synthase isoforms in sheep with smoke inhalation and burn injury.
abstract: Previous studies have indicated increased plasma levels of inducible nitric oxide synthase in lung. This study further examines the pulmonary expression of nitric oxide synthase (NOS) isoforms in an ovine model of acute lung injury induced by smoke inhalation and burn injury (S+B injury). Female range bred sheep (4 per group) were sacrificed at 4, 8, 12, 24, and 48 hours after injury and immunohistochemistry was performed in tissues for various NOS isoforms. The study indicates that in uninjured sheep lung, endothelial (eNOS) is constitutively expressed in the endothelial cells associated with the airways and parenchyma, and in macrophages. Similarly, neuronal (nNOS) is constitutively present in the mucous cells of the epithelium and in neurons of airway ganglia. In uninjured lung, inducible (iNOS) was present in bronchial secretory cells and macrophages. In tissue after S+B injury, new expression of iNOS was evident in bronchial ciliated cells, basal cells, and mucus gland cells. In the parenchyma, a slight increase in iNOS immunostaining was seen in type I cells at 12 and 24 hours after injury only. Virtually no change in eNOS or nNOS was seen after injury.
authors.affiliation: Department of Pathology, University of Texas Medical Branch and Shriners Hospitals for Children, Galveston, Texas 77550, USA. rocox@utmb.edu
authors.forename: Robert A
authors.initials: RA
authors.lastname: Cox
authors.forename: Sam
authors.initials: S
authors.lastname: Jacob
authors.forename: Gloria
authors.initials: G
authors.lastname: Oliveras
authors.forename: Kazunori
authors.initials: K
authors.lastname: Murakami
authors.forename: Perenlei
authors.initials: P
authors.lastname: Enkhbaatar
authors.forename: Lillian
authors.initials: L
authors.lastname: Traber
authors.forename: Frank C
authors.initials: FC
authors.lastname: Schmalstieg
authors.forename: David N
authors.initials: DN
authors.lastname: Herndon
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Traber
authors.forename: Hal K
authors.initials: HK
authors.lastname: Hawkins
mesh_terms: D000818:Animals; D001980:Bronchi / Q000201:enzymology; D002056:Burns / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D005260:Female; D007700:Kinetics; D008168:Lung / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology; D023421:Models, Animal; D009474:Neurons / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052248:Nitric Oxide Synthase Type I / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis; D020545:Respiratory Mucosa / Q000201:enzymology; D012756:Sheep; D015208:Smoke Inhalation Injury / Q000201:enzymology*; D014018:Tissue Distribution
chemical_list: D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III