title: Comparison of density and morphology of neuroblasts in the dentate gyrus among variously aged dogs, German shepherds.
abstract: Neural progenitor cells in the dentate subgranular zone (SGZ) produce new cells all through life via proliferation. We observed differences in neuroblasts using doublecortin (DCX, a marker of neuronal differentiation) in the SGZ in variously aged dogs (German shepherds). In the 1-year-old group, DCX-immunoreactive (+) cells were abundantly observed in the SGZ: These cells had processes extending into the molecular layer. In the 3-year-old group, DCX(+) cells were decreased by 53.0% compared to that in the 1-year-old group, and DCX(+) processes were also markedly decreased. In the 6 and 8-year-old groups, DCX(+) cells were decreased by 34.3% and 62.9% vs. the pre-aged group. In the 8-year-old group, DCX(+) cells were decreased by 86.4% compared to that in the 1-year-old group. In the 12-year-old group, DCX(+) cells and processes were nearly disappeared. This suggests that DCX(+) cells processes significantly decreased at 3 years in the SGZ of the German shepherd.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000367:Age Factors; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology*; D018891:Dentate Gyrus / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D004285:Dogs / Q000033:anatomy & histology*; D000089622:Doublecortin Domain Proteins; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009024:Morphogenesis; D009420:Nervous System / Q000196:embryology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides