title: Cytoskeleton disorganization during apoptosis induced by curcumin in A549 lung adenocarcinoma cells.
abstract: Several studies have shown that curcumin can induce apoptosis and inhibit growth in human A549 lung adenocarcinoma cells. However, the mechanism is not completely understood yet. In the present study, we investigated the in vitro effect of curcumin on cell viability, apoptosis and disorganization of the actin cytoskeleton in A549 cells. Our results showed that curcumin significantly inhibited the viability of A549 cells in a dose- and time-dependent manner by induced apoptosis. The apoptotic process was associated with a disorganization of the architecture of actin microfilaments and a decrease in the levels of F-actin. DMSO-treated control cells exhibited a well-defined F-actin network that was mainly organized into stress fibers. The actin fibers in cells treated with curcumin or the positive control drug cytochalasin B were disorganized, disassembled, or disrupted, however, the disorganization of actin fibers and apoptosis could be prevented by phalloidin, an F-actin stabilizing compound. Thus, these results demonstrated that actin filament disorganization might play a central role in the curcumin-induced apoptosis of A549 cells.
authors.affiliation: Department of Respiratory Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hang Zhou, P. R. China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Chen
authors.forename: Guohua
authors.initials: G
authors.lastname: Lu
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yingke
authors.initials: Y
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Zhongyong
authors.initials: Z
authors.lastname: Jiang
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhan
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wu
authors.forename: Jianying
authors.initials: J
authors.lastname: Zhou
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism; D000230:Adenocarcinoma / Q000473:pathology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D030024:Curcuma; D003474:Curcumin / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology / Q000627:therapeutic use; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D010590:Phalloidine / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011042:Poisons / Q000494:pharmacology
chemical_list: D000199:Actins; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D011042:Poisons; D010590:Phalloidine; D003571:Cytochalasin B; D003474:Curcumin