[Peptide CY11 conjugated polyethylenimine-beta-cyclodextrin for gene delivery].
To develop a novel non-viral gene delivery vector CY11-PEI-beta-CyD and to test its gene transfection efficiency.



CY11 (CGMQLPLATWY) was conjugated to polyethylenimine-beta-cyclodextrin to form CY11-PEI-beta-CyD with a cross-linker [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. (1)H-NMR and TGA were used to confirm the structure of vector. The DNA condensing ability of CY11-PEI-beta-CyD was investigated by gel retardation assay. Cytotoxicity of CY11-PEI-beta-CyD was determined by MTT assay and transfection efficiency was investigated in COS-7, Hela and B16 cells.



CY11 was conjugated onto PEI-beta-CyD successfully, confirmed by(1)H NMR and TGA. The novel vector effectively condensed DNA at N/P ratio of 4îIt showed low cytotoxicity up to the concentration was 160 Mgr;g/ml. The transfection efficiency was 17-fold higher than that of PEI 25 kDa at N/P ratio of 20.



The novel vector CY11 -PEI-beta-CyD with low cytotoxic and high transfection efficiency may be used as a potential carrier for gene delivery.
Hu
Yi-Ping
YP
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
Jiang
Qi-Ying
QY
Chen
Dan
D
Zhao
Dan-Jun
DJ
Lu
Xiao
X
Yu
Hai
H
Wang
Qing-Qing
QQ
Zhou
Jun
J
Hu
Xiu-Rong
XR
Tang
Gu-Ping
GP
D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D017468:Receptors, Fibroblast Growth Factor / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D010446:Peptide Fragments; D017468:Receptors, Fibroblast Growth Factor; D047392:beta-Cyclodextrins; D011094:Polyethyleneimine; C031215:betadex