Cytotoxicity of cardenolides and cardenolide glycosides from Asclepias curassavica.
A new cardenolide, 12beta,14beta-dihydroxy-3beta,19-epoxy-3alpha-methoxy-5alpha-card-20(22)-enolide (6), and a new doubly linked cardenolide glycoside, 12beta-hydroxycalotropin (13), together with eleven known compounds, coroglaucigenin (1), 12beta-hydroxycoroglaucigenin (2), calotropagenin (3), desglucouzarin (4), 6'-O-feruloyl-desglucouzarin (5), calotropin (7), uscharidin (8), asclepin (9), 16alpha-hydroxyasclepin (10), 16alpha-acetoxycalotropin (11), and 16alpha-acetoxyasclepin (12), were isolated from the aerial part of ornamental milkweed, Asclepias curassavica and chemically elucidated through spectral analyses. All the isolates were evaluated for their cytotoxic activity against HepG2 and Raji cell lines. The results showed that asclepin (9) had the strongest cytotoxic activity with an IC(50) value of 0.02 microM against the two cancer cell lines and the new compound 13 had significant cytotoxic activity with IC(50) values of 0.69 and 1.46 microM, respectively.
Li
Jun-Zhu
JZ
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
Qing
Chen
C
Chen
Chang-Xiang
CX
Hao
Xiao-Jiang
XJ
Liu
Hai-Yang
HY
D031108:Asclepias / Q000737:chemistry*; D002298:Cardenolides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D002460:Cell Line; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006801:Humans
C541280:12,14-dihydroxy-3,19-epoxy-3-methoxy-5-card-20(22)-enolide; C541281:12-hydroxycalotropin; D002298:Cardenolides; D006027:Glycosides