title: Is the intravascular administration of mesenchymal stem cells safe? Mesenchymal stem cells and intravital microscopy.
abstract: We investigated the kinetics of human mesenchymal stem cells (MSCs) after intravascular administration into SCID mouse cremaster vasculature by intravital microscopy. MSCs were injected into abdominal aorta through left femoral artery at two different concentrations (1 x 10(6) or 0.2 x 10(6) cell). Arterial blood velocity decrease by 60 and 18% 1 min after high/low dose MSCs injection respectively. The blood microcirculation was interrupted after 174+/-71 and 485+/-81 s. Intravital microscopy observation and histopathologic analysis of cremaster muscles indicated MSCs were entrapped in capillaries in both groups. 40 and 25% animals died of pulmonary embolism respectively in both high and low MSCs dose groups, which was detected by histopathologic analysis of the lungs. Intraarterial MSCs administration may lead to occlusion in the distal vasculature due to their relatively large cell size. Pulmonary sequestration may cause death in small laboratory animals. MSCs should be used cautiously for intravascular transplantation.
authors.affiliation: Department of Cardiac Surgery, Medical Faculty, University of Rostock Schillingallee 35, 18055 Rostock, Germany.
authors.forename: Dario
authors.initials: D
authors.lastname: Furlani
authors.forename: Murat
authors.initials: M
authors.lastname: Ugurlucan
authors.forename: LeeLee
authors.initials: L
authors.lastname: Ong
authors.forename: Karen
authors.initials: K
authors.lastname: Bieback
authors.forename: Erik
authors.initials: E
authors.lastname: Pittermann
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Westien
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Can
authors.initials: C
authors.lastname: Yerebakan
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Li
authors.forename: Ralf
authors.initials: R
authors.lastname: Gaebel
authors.forename: Ren-ke
authors.initials: RK
authors.lastname: Li
authors.forename: Brigitte
authors.initials: B
authors.lastname: Vollmar
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001160:Arterioles / Q000473:pathology / Q000503:physiopathology; D001783:Blood Flow Velocity / Q000502:physiology; D048429:Cell Size; D006801:Humans; D007269:Injections, Intra-Arterial; D007511:Ischemia; D008168:Lung / Q000098:blood supply / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects* / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D008853:Microscopy / Q000379:methods; D018482:Muscle, Skeletal / Q000098:blood supply; D011655:Pulmonary Embolism / Q000209:etiology* / Q000473:pathology; D013923:Thromboembolism / Q000209:etiology* / Q000473:pathology