title: Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus.
abstract: Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll--interleukin 1--receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24h post-injection and lasted until the fish became moribund (P<0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow.
authors.affiliation: Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, No. 22 Xinong Rd., Yangling, China. su.jianguo@gmail.com
authors.forename: Jianguo
authors.initials: J
authors.lastname: Su
authors.forename: Chunrong
authors.initials: C
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Xiong
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.forename: Zuoyan
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D003530:Cyprinidae / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012087:Reoviridae / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D051197:Toll-Like Receptor 4