Differential interference contrast microscope using photonic crystals for phase imaging and three-dimensional shape reconstruction.
We present a differential interference contrast microscope using photonic crystals capable of real-time capture of both phase and amplitude components independently without moving parts. Unlike previous methods using rotating polarizers to discriminate each component, we propose using a special camera equipped with an arrayed polarizer whose instant polarization measurement allows real-time acquisition of the phase gradient information. A two-image algorithm is used to reconstruct the phase two-dimensional distribution of biological samples from the gradient information with a transmission-type microscope. We also talk about deducing a sample's three-dimensional shape for a reflection-type microscope. The efficiency of the method is demonstrated experimentally.
Fabre
Laurent
L
Graduate School of Information Sciences, Tohoku University, 6-6-04 Aramaki Aza Aoba, Aoba-ku,Sendai, Miyagi 980-8579, Japan. lfabre@upstream-lab.com
Inoue
Yoshihiko
Y
Aoki
Takafumi
T
Kawakami
Shojiro
S
D000465:Algorithms; D003199:Computer Systems; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D010781:Photography / Q000295:instrumentation