title: A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana x P. bolleana).
abstract: The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4 degrees C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 microM). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana x P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.
authors.affiliation: Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Lin
authors.forename: Bai-Chen
authors.initials: BC
authors.lastname: Wang
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Wang
authors.forename: Gui-Feng
authors.initials: GF
authors.lastname: Liu
authors.forename: Chuan-Ping
authors.initials: CP
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017796:Caulimovirus / Q000235:genetics; D003080:Cold Temperature; D003890:Desiccation; D020869:Gene Expression Profiling; D009997:Osmotic Pressure; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology*; D032107:Populus / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological; D032363:Tamaricaceae / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D000040:Abscisic Acid