title: Identification of QTLs that modify peripheral neuropathy in NOD.H2b-Pdcd1-/- mice.
abstract: The non-obese diabetic (NOD) mouse strain is prone to developing various autoimmune syndromes including type I diabetes mellitus (T1DM), sialadenitis, thyroiditis and pancreatitis. Although the genetic basis of T1DM has been extensively analyzed, genetic factors that modify the other autoimmune phenotypes are largely unknown. We have recently reported that NOD mice with anti-diabetogenic MHC haplotype (H-2(b)) and programmed cell death 1 (PD-1) deficiency (NOD.H2(b)-Pdcd1(-/-) mice) are protected from T1DM but develop various tissue-specific autoimmune diseases including peripheral neuropathy due to autoimmune neuritis, sialadenitis and gastritis. In the present study, we generated [(C57BL/6 x NOD.H2(b))(F1) x NOD-H2(b)](BC1)-Pdcd1(-/-) mice to screen non-MHC quantitative trait loci (QTLs) that modify autoimmune phenotypes other than T1DM. We identified seven QTLs for peripheral neuropathy and neuritis, one QTL for insulitis, four QTLs for gastritis, two QTLs for sialadenitis and seven QTLs for vasculitis throughout the genome and designated them as Annp loci for autoimmunity due to polymorphisms of non-MHC genes in NOD mice and PD-1 deficiency. Annp1, 5, 6 and 7 overlapped with reported loci for T1DM (Idd3, 9, 15 and 2, respectively), suggesting that these loci modify not only T1DM but also other autoimmune phenotypes. NOD allele was promotive at 9 of 14 Annp loci, while NOD allele was protective at the other loci. Half of Annp loci associated with a single phenotype, while the other seven loci associated with more than two phenotypes. These results indicate that NOD genetic background harbors various QTLs that modify autoimmune phenotypes either by organ-specific or by organ-non-specific manner.
authors.affiliation: Department of Immunology and Genomic Medicine, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Fang
authors.initials: F
authors.lastname: Jiang
authors.forename: Taku
authors.initials: T
authors.lastname: Yoshida
authors.forename: Fumio
authors.initials: F
authors.lastname: Nakaki
authors.forename: Seigo
authors.initials: S
authors.lastname: Terawaki
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Chikuma
authors.forename: Yu
authors.initials: Y
authors.lastname: Kato
authors.forename: Il-Mi
authors.initials: IM
authors.lastname: Okazaki
authors.forename: Tasuku
authors.initials: T
authors.lastname: Honjo
authors.forename: Taku
authors.initials: T
authors.lastname: Okazaki
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000276:immunology; D015551:Autoimmunity / Q000235:genetics* / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005756:Gastritis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D020022:Genetic Predisposition to Disease; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D009443:Neuritis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D010195:Pancreatitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D010523:Peripheral Nervous System Diseases / Q000235:genetics* / Q000473:pathology; D061026:Programmed Cell Death 1 Receptor; D040641:Quantitative Trait Loci; D012793:Sialadenitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D014657:Vasculitis / Q000235:genetics / Q000276:immunology / Q000473:pathology
chemical_list: D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor