Simultaneous measurement of diazolidinyl urea, urea, and allantoin in cosmetic samples by hydrophilic interaction chromatography.
A new HPLC method for simultaneous measurement of diazolidinyl urea (DU), urea, and allantoin by hydrophilic interaction chromatography using a column packed with triazol-bonded silica particles is described. The calibration curves of DU, urea, and allantoin were linear over the ranges 2.5-125.0, 30-1250, and 0.25-18.75mg/L, respectively. The recoveries of DU, urea, and allantoin from homemade cosmetic samples ranged from 92.84% to 101.69%, 98.19% to 103.22%, and 95.75% to 102.09%, respectively. The peak relative standard deviation (RSD) values for the recovery tests of 3 concentrations/5 replicates were 3.03% for all compounds. In day-to-day measurement of recovery tests from homemade lotions over 3 consecutive days, the RSDs were less than 2.5% in all cases. This method was well validated and would be helpful for quickly analyzing these compounds in cosmetic samples.
Doi
Takahiro
T
Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537-0025, Japan. tdoi@iph.pref.osaka.jp
Kajimura
Keiji
K
Takatori
Satoshi
S
Fukui
Naoki
N
Taguchi
Shuzo
S
Iwagami
Shozo
S
D000481:Allantoin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000032:analysis*; D012680:Sensitivity and Specificity; D014508:Urea / Q000031:analogs & derivatives* / Q000032:analysis*
D003358:Cosmetics; D000481:Allantoin; D014508:Urea; C044982:diazolidinylurea