Preparation and pH stability of ferrous glycinate liposomes.
Ferrous glycinate liposomes were prepared by reverse phase evaporation method. The effects of cholesterol, Tween 80, ferrous glycinate concentration, hydrating medium, pH of hydrating medium, and sonication strength on the encapsulation efficiency of liposomes were investigated. Encapsulation efficiency was significantly influenced by the different technique parameters. Ferrous glycinate liposomes might be obtained with high encapsulation efficiency of 84.80% under the conditions of optimized technique parameters. The zeta potential and average particle size of liposomes in the hydrating medium of pH 7.0 were 9.6 mV and 559.2 nm, respectively. The release property of ferrous glycinate liposomes in vitro was investigated in simulated gastrointestinal juice. A small amount of ferrous glycinate was released from liposomes in the first 4 h in simulated gastrointestinal juice. The mean diameters of liposomes increased from 559.2 to 692.9, 677.8, and 599.3 nm after incubation in simulated gastrointestinal juice of pH 1.3, 7.5, and 7.5 in the presence of bile salts, respectively. Results showed that the stability of ferrous glycinate in strong acid environment was greatly improved by encapsulation in liposomes, which protected ferrous glycinate from disrupting the extracapsular environment by lipid bilayer. The bioavailability of ferrous glycinate, as the iron source for biological activity including hemoglobin formation, may be increased. The ferrous glycinate liposomes may be a kind of promising iron fortifier.
Ding
Baomiao
B
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, Jiangsu 214122, China.
Xia
Shuqin
S
Hayat
Khizar
K
Zhang
Xiaoming
X
D002784:Cholesterol / Q000032:analysis; D019587:Dietary Supplements; D004339:Drug Compounding; D004355:Drug Stability; D005296:Ferrous Compounds / Q000008:administration & dosage / Q000737:chemistry*; D005750:Gastric Juice / Q000737:chemistry; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000138:chemical synthesis* / Q000737:chemistry; D010316:Particle Size; D011136:Polysorbates / Q000032:analysis
D005296:Ferrous Compounds; D008081:Liposomes; D011136:Polysorbates; D002784:Cholesterol; D005998:Glycine