title: Monocyte differentiation is controlled by MyD88 after mouse orthotopic lung transplantation.
abstract: In lung grafts, ischemia-reperfusion signals rapidly induce the recruitment and differentiation of host monocytes into macrophages and dendritic cells. The nature of ischemia-reperfusion signals are antigen independent, but have been hypothesized to initiate Toll-like receptor (TLR) and interleukin (IL)-1R-mediated signaling pathways that are thought to potentiate alloimmune responses. We wondered whether MyD88, an adaptor molecule critical for both TLR and IL-1R-mediated inflammatory responses, regulated monocyte differentiation in a mouse model of vascularized orthotopic lung transplantation. Orthotopic left lung transplants were performed in the following syngeneic combinations: CD45.1(+) B6 --> CD45.2(+) MyD88(-/-) and CD45.1(+) B6 --> CD45.2(+) B6. One day later, recipient-derived dendritic cells and macrophage numbers were assessed in the bronchiolar lavage by FACS analysis. Compared with the bronchiolar lavage of wildtype recipients, MyD88(-/-) recipients had lower numbers of dendritic cells in lung graft airways that were of recipient origin. Lower numbers of newly differentiated lung graft dendritic cells was coincident with the appearance of higher numbers of undifferentiated monocytes in the lung airways of MyD88(-/-) recipients as compared with wild-type recipients. Moreover, adoptive transfer experiments demonstrated that MyD88(-/-) monocytes were poorer at differentiating into lung dendritic cells as compared with wild-type monocytes. Taken together, these data show that MyD88 regulates graft-infiltrating monocyte differentiation and suggests a mechanism by which TLR/IL-1R-signaling pathways control adaptive responses in lung allografts through controlling monocyte fate.
authors.affiliation: Division of Cardiothoracic Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, Missourri 63110-1013, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sugimoto
authors.forename: X
authors.initials: X
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Okazaki
authors.forename: J
authors.initials: J
authors.lastname: Lai
authors.forename: J R
authors.initials: JR
authors.lastname: Tietjens
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: G A
authors.initials: GA
authors.lastname: Patterson
authors.forename: A S
authors.initials: AS
authors.lastname: Krupnick
authors.forename: D
authors.initials: D
authors.lastname: Kreisel
authors.forename: A E
authors.initials: AE
authors.lastname: Gelman
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D016040:Lung Transplantation / Q000276:immunology / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D053594:Myeloid Differentiation Factor 88 / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology / Q000502:physiology
chemical_list: C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse