title: Microarray analysis of gene expression in soybean roots susceptible to the soybean cyst nematode two days post invasion.
abstract: Soybean root cells undergo dramatic morphological and biochemical changes during the establishment of a feeding site in a compatible interaction with the soybean cyst nematode (SCN). We constructed a cDNA microarray with approximately 1,300 cDNA inserts targeted to identify differentially expressed genes during the compatible interaction of SCN with soybean roots 2 days after infection. Three independent biological replicates were grown and inoculated with SCN, and 2 days later RNA was extracted for hybridization to microarrays and compared to noninoculated controls. Statistical analysis indicated that approximately 8% of the genes monitored were induced and more than 50% of these were genes of unknown function. Notable genes that were more highly expressed 2 days after inoculation with SCN as compared to noninoculated roots included the repetitive proline-rich glycoprotein, the stress-induced gene SAM22, ss-1,3-endoglucanase, peroxidase, and those involved in carbohydrate metabolism, plant defense, and signaling.
authors.forename: R
authors.initials: R
authors.lastname: Khan
authors.forename: N
authors.initials: N
authors.lastname: Alkharouf
authors.forename: H
authors.initials: H
authors.lastname: Beard
authors.forename: M
authors.initials: M
authors.lastname: Macdonald
authors.forename: I
authors.initials: I
authors.lastname: Chouikha
authors.forename: S
authors.initials: S
authors.lastname: Meyer
authors.forename: J
authors.initials: J
authors.lastname: Grefenstette
authors.forename: H
authors.initials: H
authors.lastname: Knap
authors.forename: B
authors.initials: B
authors.lastname: Matthews