Validated HPLC method for the standardization of Phyllanthus niruri (herb and commercial extracts) using corilagin as a phytochemical marker.
Phyllanthus niruri L., commonly known in Brazil as 'quebra-pedra', has long been used in the treatment of diverse diseases and especially urolithiasis. The therapeutic effects of P. niruri are attributed to various compounds present in the plant, including the hydrolysable tannin corilagin. In the present study, high-performance liquid chromatography (HPLC-/PAD) profiles of leaves and commercial extracts of P. niruri were examined and three compounds, found to be present in all of the samples studied, were isolated by open column chromatography over C18)silica gel followed by preparative HPLC. These compounds were identified by nuclear magnetic resonance as corilagin, rutin and ethyl 3,4,5-trihydroxybenzoate. Corilagin, which has been proposed as a phytochemical marker for P. niruri, was employed as an external standard in the development and validation of a rapid and efficient qualitative and quantitative HPLC assay for the analyte. The method may be applied in the standardization of herbs and phytomedicines commercialized in Brazil as quebra-pedra.
Colombo
Renata
R
Instituto de Química, Universidade Estadual Paulista, Araraquara, SP, Brazil. renatacolomb@gmail.com
de L Batista
Andrea N
AN
Teles
Helder L
HL
Silva
Geraldo H
GH
Bomfim
Giovani C C
GC
Burgos
Rosilene C R
RC
Cavalheiro
Alberto J
AJ
da Silva Bolzani
Vanderlan
V
Silva
Dulce Helena S
DH
Pelícia
Clícia R
CR
Guimarães
Fabiana M
FM
Heimberg
Maria Célia H
MC
D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis*; D047348:Hydrolyzable Tannins; D009682:Magnetic Resonance Spectroscopy; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013997:Time Factors
D005960:Glucosides; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; C048734:ethyl gallate; D012431:Rutin; C049096:corilagin; D005707:Gallic Acid