title: Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells.
abstract: Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.
authors.affiliation: Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, PR China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiu-Qing
authors.initials: XQ
authors.lastname: Fu
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Guo
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Gao
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Yu
authors.forename: Heng-Liang
authors.initials: HL
authors.lastname: Shi
authors.forename: Xing-Zhi
authors.initials: XZ
authors.lastname: Wang
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Xiong
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D051379:Mice; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C409064:Myo10 protein, mouse; D011993:Recombinant Fusion Proteins; D007987:Gonadotropin-Releasing Hormone; D009218:Myosins