title: cDNA cloning and life-cycle stage-specific expression of coronin from Physarum polycephalum.
abstract: Coronin cDNA was cloned from the plasmodia of Physarum polycephalum. The amino acid sequence deduced from the cDNA was comprised of 449 residues and showed 60% identity to that of Dictyostelium discoideum coronin. Southern blot analysis suggested that the coronin gene present in the P. polycephalum genome might be a single copy. Coronin was expressed in diploid plasmodia, while it was not detected in haploid amoebae or spores.
authors.affiliation: Department of Biochemistry, Faculty of Science, Okayama University of Science, Okayama, Japan. minami@dbc.ous.ac.jp
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Minami
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Masato
authors.initials: M
authors.lastname: Hayase
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Sakaguchi
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Yubisui
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D008018:Life Cycle Stages / Q000235:genetics*; D008969:Molecular Sequence Data; D016839:Physarum polycephalum / Q000235:genetics* / Q000254:growth & development*; D016415:Sequence Alignment; D054378:Acetogenins
chemical_list: D015107:4-Butyrolactone; D018076:DNA, Complementary; C438920:coronin acetogenin; D054378:Acetogenins