title: The complete mitochondrial genome sequence of Geisha distinctissima (Hemiptera: Flatidae) and comparison with other hemipteran insects.
abstract: The complete nucleotide sequence of the mitochondrial genome (mitogenome) of Geisha distinctissima (Hemiptera: Flatidae) has been determined in this study. The genome is a circular molecule of 15,971 bp with a total A+T content of 75.1%. The gene content, order, and structure are consistent with the Drosophila yakuba genome structure and the hypothesized ancestral arthropod genome arrangement. All 13 protein-coding genes are observed to have a putative, inframe ATR methionine or ATT isoleucine codons as start signals. Canonical TAA and TAG termination codons are found in nine protein-coding genes, and the remaining four (cox1, atp6, cox3, and nad4) have incomplete termination codons. The anticodons of all transfer RNA (tRNAs) are identical to those observed in D. yakuba and Philaenus spumarius, and can be folded in the form of a typical clover-leaf structure except for tRNA(Ser(AGN)). The major non-coding region (the A+T-rich region or putative control region) between the small ribosomal subunit and the tRNA(Ile) gene includes two sets of repeat regions. The first repeat region consists of a direct 152-bp repetitive unit located near the srRNA gene end, and the second repeat region is composed of a direct repeat unit of 19 bp located toward tRNA(Ile) gene. Comparisons of gene variability across the order suggest that the gene content and arrangement of G. distinctissima mitogenome are similar to other hemipteran insects.
authors.affiliation: Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Song
authors.forename: Aiping
authors.initials: A
authors.lastname: Liang
mesh_terms: D020856:AT Rich Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D018388:Codon, Terminator; D017931:DNA Primers / Q000737:chemistry; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial / Q000235:genetics*; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D006430:Hemiptera / Q000145:classification / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D018388:Codon, Terminator; D017931:DNA Primers; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial; D019476:Insect Proteins; D012335:RNA, Ribosomal; D012313:RNA; D012343:RNA, Transfer