[Isolation-preparation and determination of chromones from Saposhnikovia divaricata].
OBJECTIVE
To compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.


METHODS
The separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).


RESULTS
All the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.


CONCLUSIONS
The method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.
Zhang
Cun
C
Institute of Chinese Materia Madica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Xiao
Yong-qing
YQ
Li
Li
L
Pang
Zhen
Z
Li
Gui-liu
GL
D019661:Apiaceae / Q000737:chemistry*; D002867:Chromones / Q000032:analysis* / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*
D002867:Chromones