title: [Study the effect of antisense MMP-2 by transfection of ad-aMMP-2 on vascular endothelial cells from human proferating hemangiomas in vitro].
abstract: OBJECTIVE
  To investigate the effect of antisense MMP-2 by transfecting the Ad-aMMP-2 on the vascular endothelial cells (VEC) derived from human proferating hemangiomas in vitro.
  
  
  METHODS
  Three groups. Group M199, Group Ad-GFP and Group Ad-aMMP-2 were tested in this study with the 100 multiplicity of infection (MOI). Reverse transcription PCR (RT-PCR), Western Blotting and Gelatin Zymography analyses were applied to evaluate the level of endogenous MMP-2 expression.
  
  
  RESULTS
  The decreased expression level of endogenous MMP-2 mRNA and protein excretion of MMP-2 and Gelatin Zymography analyses of liveness of Group Ad-aMMP-2 were observed by comparison with Group M199 and Group Ad-GFP (P<0.05).
  
  
  CONCLUSIONS
  Ad-aMMP-2 could inhibit the secretion of MMP-2 from VEC in vitro.
authors.affiliation: Department of Reconstructive and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Fan-wei
authors.initials: FW
authors.lastname: Zeng
authors.forename: Yin-ga
authors.initials: YG
authors.lastname: Cen
authors.forename: Xiao-xue
authors.initials: XX
authors.lastname: Liu
authors.forename: Xue-wen
authors.initials: XW
authors.lastname: Xu
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Chen
authors.forename: Zheng-yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Wu
mesh_terms: D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics / Q000494:pharmacology; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006392:Hemangioma, Cavernous / Q000473:pathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000473:pathology; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2