Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPalpha, PPARgamma2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.
Taura
Daisuke
D
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan.
Noguchi
Michio
M
Sone
Masakatsu
M
Hosoda
Kiminori
K
Mori
Eisaku
E
Okada
Yohei
Y
Takahashi
Kazutoshi
K
Homma
Koichiro
K
Oyamada
Naofumi
N
Inuzuka
Megumi
M
Sonoyama
Takuhiro
T
Ebihara
Ken
K
Tamura
Naohisa
N
Itoh
Hiroshi
H
Suemori
Hirofumi
H
Nakatsuji
Norio
N
Okano
Hideyuki
H
Yamanaka
Shinya
S
Nakao
Kazuwa
K
D017667:Adipocytes / Q000187:drug effects / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D000071317:Nanog Homeobox Protein; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D039904:Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D015415:Biomarkers; D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D005353:Fibronectins; D018398:Homeodomain Proteins; D020738:Leptin; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D047495:PPAR gamma; D010455:Peptides; C008973:polyornithine