Effective antibody production by reusing culture medium previously used in antibody purification.
Culture medium used in antibody purification contains growth factors, but is usually discarded after antibody purification. We examined to determine whether the growth factors can be (i) recovered and (ii) used in subsequent cell culture. Using this medium enhanced the survival of hybridoma cells and improved antibody production. Culture medium previously used in antibody purification offers an attractive resource for mammalian cell culture.
Ogawa
Akiko
A
Department of Chemistry and Biochemistry, Suzuka National College of Technology, Suzuka, Mie, Japan. ogawa@chem.suzuka-ct.ac.jp
Takada
Naoki
N
Terada
Satoshi
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects; D051379:Mice
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned