GAMETOPHYTIC FACTOR 1, involved in pre-mRNA splicing, is essential for megagametogenesis and embryogenesis in Arabidopsis.
RNA biogenesis is essential and vital for accurate expression of genes. It is obvious that cells cannot continue normal metabolism when RNA splicing is interfered with. sgt13018 is such a mutant, with partial loss of function of GAMETOPHYTIC FACTOR 1 (GFA1); a gene likely involved in RNA biogenesis in Arabidopsis. The mutant is featured in the phenotype of diminished female gametophyte development at stage FG5 and is associated with the arrest of early embryo development in Arabidopsis. Bioinformatics data showed that homologs of gene GFA1 in yeast and human encode putative U5 snRNP-specific proteins required for pre-mRNA splicing. Furthermore, the result of yeast two-hybrid assay indicated that GFA1 physically interacted with AtBrr2 and AtPrp8, the putative U5 snRNP components, of Arabidopsis. This investigation suggests that GFA1 is involved in mRNA biogenesis through interaction with AtBrr2 and AtPrp8 and functions in megagametogenesis and embryogenesis in plant.
Liu
Man
M
Key Laboratory of Molecular and Developmental Biology, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yuan
Li
L
Liu
Nai-You
NY
Shi
Dong-Qiao
DQ
Liu
Jie
J
Yang
Wei-Cai
WC
D017360:Arabidopsis / Q000166:cytology / Q000196:embryology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development; D005718:Gametogenesis; D018506:Gene Expression Regulation, Plant; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D050505:Mutant Proteins / Q000235:genetics / Q000302:isolation & purification; D009154:Mutation / Q000235:genetics; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D011485:Protein Binding; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000378:metabolism; D012639:Seeds / Q000166:cytology / Q000196:embryology
D029681:Arabidopsis Proteins; C541497:GFA1 protein, Arabidopsis; D050505:Mutant Proteins; D010445:Peptide Elongation Factors; D012322:RNA Precursors; D017415:Ribonucleoprotein, U5 Small Nuclear