title: Desmoglein ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris.
abstract: Anti-desmoglein 3 and 1 autoantibodies are involved in the pathogenesis of pemphigus diseases. Our objective was to assess the value of ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris. Based on clinical presentation and histopathologic confirmation for the diagnosis of the pemphigus, 38 patients took part in the study. Sera of the patients were tested by desmoglein 1 and desmoglein 3 ELISA. Also, direct immunofluorescence was performed for all patients which revealed positive results in 36 patients (94.7%). ELISA was positive in 37 of 38 pemphigus patients (Sensitivity: 97.3%). The relationship between desmoglein 1 index values and skin severity was statistically significant (p<0.05). Desmoglein 3 index values increased with oral severity although this was not statistically significant. Iranian patients similar to Indian patients had higher positive anti-desmoglein 1 autoantibodies. Desmoglein-ELISA test is appropriate in the diagnosis of pemphigus. Desmoglein 1 index value is statistically correlated with the severity of pemphigus vulgaris.
authors.affiliation: Department of Dermatology, Autoimmune Bullous Diseases Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mortazavi
authors.forename: Majid
authors.initials: M
authors.lastname: Shahdi
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Amirzargar
authors.forename: Zahra S
authors.initials: ZS
authors.lastname: Naraghi
authors.forename: Mahin
authors.initials: M
authors.lastname: Valikhani
authors.forename: Maryam
authors.initials: M
authors.lastname: Daneshpazhooh
authors.forename: Amir
authors.initials: A
authors.lastname: Vasheghani-Farahani
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Sedaghat
authors.forename: Cheyda
authors.initials: C
authors.lastname: Chams-Davatchi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D051183:Desmoglein 1 / Q000276:immunology; D051184:Desmoglein 3 / Q000276:immunology; D051182:Desmogleins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000473:pathology; D010392:Pemphigus / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000473:pathology; D055815:Young Adult
chemical_list: D001323:Autoantibodies; C495297:DSG1 protein, human; C071894:DSG3 protein, human; D051183:Desmoglein 1; D051184:Desmoglein 3; D051182:Desmogleins