Food deprivation induces monocarboxylate transporter 2 expression in the brainstem of female rat.
Ketone bodies are considered to act as a signal to suppress gonadotropin release during negative energy balance. The present study examined the effect of 48-h fasting on the mRNA expressions of monocarboxylate transporter 1 (MCT1) and MCT2, which are involved in ketone body transport, in several brain regions. Quantitative real-time RT-PCR analysis showed that the MCT2 mRNA levels were significantly increased by 48-h fasting in the area postrema-solitary tract nucleus (AP-NTS) region but not the arcuate nucleus-ventromedial hypothalamic nucleus (ARC-VMH) and central gray-supragenual nucleus around the 4th ventricle (CG-SGe) regions. Fasting did not significantly affect MCT1 mRNA expression in any of the brain areas examined. Luteinizing hormone (LH) pulse frequency significantly decreased and plasma concentrations of beta-hydroxybutyric acid, a ketone body, significantly increased after 48-h fasting. The present results suggest that increased uptake of ketone bodies via MCT2 in the AP-NTS region is likely involved in the mechanism of fasting-induced suppression of LH secretion in rats.
Matsuyama
Shuichi
S
National Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization. shuichim@affrc.go.jp
Ohkura
Satoshi
S
Iwata
Kinuyo
K
Uenoyama
Yoshihisa
Y
Tsukamura
Hiroko
H
Maeda
Kei-Ichiro
K
Kimura
Koji
K
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001933:Brain Stem / Q000033:anatomy & histology / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D019344:Lactic Acid / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008954:Models, Biological; D027501:Monocarboxylic Acid Transporters / Q000235:genetics* / Q000378:metabolism; D011673:Pulsatile Flow / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
D020155:3-Hydroxybutyric Acid; D005230:Fatty Acids, Nonesterified; D019344:Lactic Acid; D007986:Luteinizing Hormone; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C091633:Slc16a7 protein, rat