title: [Determination of anthraquinone in Semen Cassiae from different regions by HPLC].
abstract: OBJECTIVE
  To determine the content of 7 anthraquinones in Semen Cassiae.
  
  
  METHODS
  A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1).
  
  
  RESULTS
  Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different.
  
  
  CONCLUSIONS
  The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.
authors.affiliation: Chongqing Academy of Chinese Materia Medcia, Chongqing 400065, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Huang
authors.forename: Dai-qun
authors.initials: DQ
authors.lastname: Weng
authors.forename: Da-jian
authors.initials: DJ
authors.lastname: Yang
mesh_terms: D000880:Anthraquinones / Q000032:analysis*; D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal