title: A Method for Isolation of Pasteuria penetrans Endospores for Bioassay and Genomic Studies.
abstract: A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations.
authors.affiliation: Center for the Biology of Nematode Parasitism, NC State University, Raleigh, NC 27695.
authors.forename: Jenora T
authors.initials: JT
authors.lastname: Waterman
authors.forename: David McK
authors.initials: DM
authors.lastname: Bird
authors.forename: Charles H
authors.initials: CH
authors.lastname: Opperman