title: Nipple aspirate fluid collection, related factors and relationship between carcinoembryonic antigen in nipple aspirate fluid and breast diseases in women in Harbin, PRC.
abstract: This study aimed to examine the factors related to the ability to obtain nipple aspirate fluids in Chinese women and to evaluate the diagnostic value of carcinoembryonic antigen (CEA) in nipple aspiration fluids. Cross-sectional and case-control studies were used to examine the factors associated with the ability to obtain nipple aspiration fluids. A matched case-control study was used to explore the association of CEA level in nipple aspiration fluids with breast disease. CEA level was log10-transformed to normalize the distribution. Univariate and multivariate logistic regression were adopted to analyze the variables in relation to obtaining fluid and the association of its CEA levels and breast diseases. Receiver operating characteristic curve was used to evaluate the value of CEA levels for the detection of breast disease. The nipple aspiration fluid collection rate was 34.24% (201 of 587). Multivariate logistic regression analysis results showed the following to be significantly associated with a greater ability to obtain nipple aspiration fluids: earlier age at menarche [odds ratio (OR), 2.61; 95% confidence interval (95% CI), 1.15-5.91], lactating>12 months (OR, 2.28; 95% CI, 1.44-3.60), wet-type ear wax (OR, 3.80; 95% CI, 2.39-6.02), and fatty diet (OR, 1.56; 95% CI, 1.02-2.39). CEA levels in nipple aspiration fluids of cancerous breasts were significantly higher than those from breasts with benign disease and healthy controls (ORadj, 5.39; P<0.01). However, the sensitivity of CEAs was 35.42% and the specificity was 85.42% whereas the CEA cutoff value was 307.61 ng/mL. The area under the receiver operating characteristic curve was 0.65. The nipple aspiration fluid collection rate in Chinese native women is lower compared to non-Asian-descendant women. CEA level does not seem to be a useful diagnostic tool for early cancer detection; it can only be used in conjugation with other methods.
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, and Department of Breast Surgery, Third Affiliated Clinical Hospital of Harbin Medical University, Harbin, Heilongjiang Province, PR China. zhao_yashuang@263.net
authors.forename: Ya-Shuang
authors.initials: YS
authors.lastname: Zhao
authors.forename: Da
authors.initials: D
authors.lastname: Pang
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Ying-wei
authors.initials: YW
authors.lastname: Xue
authors.forename: Dan-ning
authors.initials: DN
authors.lastname: Gao
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Bin-you
authors.initials: BY
authors.lastname: Wang
authors.forename: Da
authors.initials: D
authors.lastname: Wang
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D001941:Breast Diseases / Q000175:diagnosis*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D009558:Nipples; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen