title: Inclusion of riboflavin in beta-cyclodextrin: a fluorimetric and absorption spectrometric study.
abstract: Formation of inclusion complexes between riboflavin and beta-cyclodextrin (beta-CD) with both 1:1 and 1:2 stoichiometry has been established by fluorimetric titration. However, in absorption spectrometric experiment, spectral change of riboflavin in the visible range could be observed only by taking beta-CD at a much higher concentration (about 100 times) than riboflavin and under such condition only 1:2 complexes could be detected. Its formation constant (K) was determined by a multiple linear regression analysis of the absorption data. The reliability of the K value was confirmed by the consistency achieved on analyzing the data at two different wavelengths.
authors.affiliation: Department of Chemistry, The University of Burdwan, Golapbag, W.B. 713104, India.
authors.forename: Dalim Kumar
authors.initials: DK
authors.lastname: Roy
authors.forename: Nipamanjari
authors.initials: N
authors.lastname: Deb
authors.forename: Bankim Chandra
authors.initials: BC
authors.lastname: Ghosh
authors.forename: Asok K
authors.initials: AK
authors.lastname: Mukherjee
mesh_terms: D000327:Adsorption; D005470:Fluorometry; D015394:Molecular Structure; D012256:Riboflavin / Q000737:chemistry*; D013053:Spectrophotometry; D017186:Titrimetry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; C031215:betadex; D012256:Riboflavin