[Beneficial effect of total flavonoids of Chrysanthemum indicum on adjuvant arthritis by induction of apoptosis of synovial fibroblasts].
OBJECTIVE
To investigate the effect on the extract of total flavonoids of Chrysanthemum indicum (TFC) on adjuvant arthritis synovial cells.


METHODS
SD rats were divided randomly into six groups including normal, model, TFC (84, 168, 336 mg x kg(-1)) and control drug Tripterygium glycosides (30 mg x kg(-1)) groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0.1 mL of the complete Freund's adjuvant into the right hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MT; The apoptosis rates of synovial cells were evaluated using TUNEL and FCM analysis.


RESULTS
TFC resulted in a dose-dependent way in inhibiting the proliferation of synovial and inducing the apoptosis of synovium and synoviocytes in vivo.


CONCLUSIONS
TFC can inhibit proliferation and induce apoptosis in synovial cells, and exert therapeutical effect on rheumatoid arthritis.
Xie
Xue-feng
XF
School of Pharmacy, Anhui Medical University, Hefei 230032, China. xuefengtt@yahoo.com.cn
Li
Jun
J
Chen
Zhen
Z
Hu
Cheng-mu
CM
Chen
Wei-wei
WW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D049109:Cell Proliferation / Q000187:drug effects; D031202:Chrysanthemum / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005419:Flavonoids / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D005419:Flavonoids