title: Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection.
abstract: Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n=7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of D- and L-Tyr derivatives were 2.9 and 2.2 micromol/L (S/N=3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution.
authors.affiliation: College of Chemistry, Sichuan University, Chengdu 610065, PR China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Bi
authors.forename: Shaorong
authors.initials: S
authors.lastname: Lei
authors.forename: Xiupei
authors.initials: X
authors.lastname: Yang
authors.forename: Zemin
authors.initials: Z
authors.lastname: Xu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yuan
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
authors.forename: Martin M F
authors.initials: MM
authors.lastname: Choi
mesh_terms: D000596:Amino Acids; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes; D008027:Light; D012996:Solutions; D013237:Stereoisomerism; D014443:Tyrosine / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D012996:Solutions; D014443:Tyrosine