title: Notch signaling downstream of foxD5 promotes neural ectodermal transcription factors that inhibit neural differentiation.
abstract: We investigated the role of the Notch signaling pathway in regulating several transcription factors that stabilize a neural fate and expand the neural plate. Increased Notch signaling in a neural lineage via a constitutively activated form (NICD) up-regulated geminin and zic2 in a cell-autonomous manner, and expanded the neural plate domains of sox11, sox2, and sox3. Loss- and gain-of-function assays show that foxD5 acts upstream of notch1 gene expression. Decreasing Notch signaling with an anti-morphic form of a Notch ligand (X-Delta-1(STU)) showed that the foxD5-mediated expansion of the sox gene neural plate domains requires Notch signaling. However, geminin and zic2 appear to be dually regulated by foxD5 and Notch1 signaling. These studies demonstrate that: (1) Notch signaling acts downstream of foxD5 to promote the expression of a subset of neural ectodermal transcription factors; and (2) Notch signaling and the foxD5 transcriptional pathway together maintain the neural plate in an undifferentiated state. Developmental Dynamics 238:1358-1365, 2009. (c) 2009 Wiley-Liss, Inc.
authors.affiliation: Department of Anatomy and Regenerative Biology, The George Washington University Medical Center, Washington, DC, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: Karen M
authors.initials: KM
authors.lastname: Neilson
authors.forename: Sally A
authors.initials: SA
authors.lastname: Moody
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D004475:Ectoderm / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D054258:Neural Plate / Q000033:anatomy & histology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C495064:FoxD5a protein, Xenopus; D016376:Oligonucleotides, Antisense; D051880:Receptors, Notch; D014157:Transcription Factors; D029867:Xenopus Proteins