High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis.
The alkaloid ricinine, an insecticide for leaf-cutting ant (Atta sexdens rubropilosa), was obtained from Ricinus communis. A two-phase solvent system composed of CH(2)Cl(2)/EtOH/H(2)O (93:35:72, v/v/v) was used for high-speed counter-current chromatographic (HSCCC) isolation of ricinine in high yield and with over 96% purity, as determined by liquid and gas chromatography-mass spectrometry (LC-MS and GC-MS). Identification of ricinine was performed by comparison of (1)H NMR, (13)C NMR and LC-MS/MS data.
Cazal
Cristiane de Melo
Cde M
Universidade Federal de São Carlos, Departamento de Química, SP, Brazil.
Batalhão
Jaqueline Raquel
JR
Domingues
Vanessa de Cássia
Vde C
Bueno
Odair Corrêa
OC
Filho
Edson Rodrigues
ER
Forim
Moacir R
MR
da Silva
Maria Fátima G Fernandes
MF
Vieira
Paulo Cezar
PC
Fernandes
João Batista
JB
D000470:Alkaloids / Q000302:isolation & purification*; D000818:Animals; D001000:Ants; D002853:Chromatography, Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007306:Insecticides / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011728:Pyridones / Q000302:isolation & purification*; D012278:Ricinus / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D000470:Alkaloids; D007306:Insecticides; D010936:Plant Extracts; D011728:Pyridones; D012997:Solvents; C019550:ricinine