Wound healing in colonic anastomosis in hypothyroidism.
Colonic anastomosis healing in hypothyroidism was evaluated. Twenty Wistar rats were separated into two groups: the study group (n = 10), which underwent total thyroidectomy to induce hypothyroidism, and the control group (n = 10). After 10 weeks, hypothyroid status was confirmed by blood tests (p < 0.0001). Three days later, a colonic anastomosis was performed, and the animals were sacrificed 1 week later. The colon containing the anastomosis was resected for bursting strength tests and histological studies. The slides were stained with hematoxylin-eosin and with Sirius red to quantify and classify the collagen. Reduced values were found for the bursting strength test (p = 0.0006) and for collagen density in the experimental group. There was a prevalence of immature type III collagen (p < 0.0001) and a decrease in mature type I collagen (p < 0.0001) in the hypothyroid group.
Thá Nassif
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AC
Division of Endocrinology and Metabolism, Department of Internal Medicine, University Hospital, Curitiba, Brasil. ana.nassif@sulbbs.com
Hintz Greca
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F
Graf
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H
Domingues Repka
J C
JC
Nassif
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LS
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000473:pathology / Q000601:surgery*; D007037:Hypothyroidism / Q000503:physiopathology*; D008297:Male; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing