[Chiral separation of bisoprolol using high performance liquid chromatography with amylose chiral stationary phase].
A high performance liquid chromatographic (HPLC) method using amylase chiral stationary phase for the separation of bisoprolol enantiomers has been developed. Tris (3,5-dimethylphenyl carbamate) amylase chiral stationary phase was used in the normal-phase mode. The effects of the concentration of organic solvents (iso-propanol and ethanol), flow rate and column temperature on the enantiomer separation were studied systematically. The best separation was obtained with hexane-ethanol-diethyl amine (88 : 12 : 0.1, v/v/v) as mobile phase at 20 degrees C. The optimal flow rate was 0.6 mL/min. The detection wavelength was set at 270 nm. By using this method, the enantiomers of bisoprolol can be separated quickly and easily.
Li
Fang
F
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. lifang198467@163.com
Li
Jiayang
J
Zhang
Huayan
H
Guo
Xingjie
X
D000688:Amylose / Q000737:chemistry*; D017298:Bisoprolol / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature
D012997:Solvents; D000688:Amylose; D017298:Bisoprolol