title: Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308.
abstract: Docosahexaenoic acid (DHA) production in Schizochytrium sp. HX-308 was evaluated by detecting enzymatic activities of ATP:citrate lyase (EC 4.1.3.8), malic enzyme (EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) at different fermentation stages. According to the analysis, a regulation strategy was proposed which reinforced acetyl-CoA and NADPH supply at a specific fermentation stage. DHA content of total fatty acids was increased from 35 to 60% by the addition of 4 g/L malic acid at the rapid lipid accumulation stage. Total lipid content also showed an apparent increase of 35% and reached 19 g/L when 40 mL ethanol/L was added at the late lipid accumulation stage.
authors.affiliation: College of Life Science and Pharmacy, Nanjing University of Technology, Gulou District, 210009 Nanjing, China.
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Xiao
authors.forename: Min
authors.initials: M
authors.lastname: Lian
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Jin
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000105:Acetyl Coenzyme A / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D003470:Culture Media; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D056890:Eukaryota / Q000254:growth & development / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000378:metabolism; D009249:NADP / Q000378:metabolism
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D004281:Docosahexaenoic Acids; D009249:NADP; D000105:Acetyl Coenzyme A; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D001275:ATP Citrate (pro-S)-Lyase