Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells.
We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus-oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA-CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA-CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells.
Tunjung
Woro Anindito Sri
WA
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi Aobaku, Sendai 981-8555, Japan. dito@bios.tohoku.ac.jp
Yokoo
Masaki
M
Hoshino
Yumi
Y
Miyake
Yuko
Y
Kadowaki
Akane
A
Sato
Eimei
E
D000818:Animals; D017209:Apoptosis; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D004789:Enzyme Activation; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D006923:Hymecromone / Q000031:analogs & derivatives / Q000494:pharmacology; D013552:Swine
D061945:Caspase Inhibitors; D018960:Hyaluronan Receptors; D006923:Hymecromone; D005640:Follicle Stimulating Hormone; D006820:Hyaluronic Acid; D020169:Caspases