Velocity-resolved 3D retinal microvessel imaging using single-pass flow imaging spectral domain optical coherence tomography.
We demonstrate in vivo velocity-resolved, volumetric bidirectional blood flow imaging in human retina using single-pass flow imaging spectral domain optical coherence tomography (SPFI-SDOCT). This technique uses previously described methods for separating moving and non-moving scatterers within a depth by using a modified Hilbert transform. Additionally, a moving spatial frequency window is applied, creating a stack of depth-resolved images of moving scatterers, each representing a finite velocity range. The resulting velocity reconstruction is validated with and strongly correlated to velocities measured with conventional Doppler OCT in flow phantoms. In vivo velocity-resolved flow mapping is acquired in healthy human retina and demonstrate the measurement of vessel size, peak velocity, and total foveal blood flow with OCT.
Tao
Yuankai K
YK
Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Durham, North Carolina 27708, USA. yt13@duke.edu
Kennedy
Kristen M
KM
Izatt
Joseph A
JA
D001783:Blood Flow Velocity; D016208:Databases, Factual; D004867:Equipment Design; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional; D008962:Models, Theoretical; D019047:Phantoms, Imaging; D012171:Retinal Vessels / Q000033:anatomy & histology / Q000502:physiology*; D012815:Signal Processing, Computer-Assisted; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data