title: Carriage of both the fnbA and fnbB genes and growth at 37 degrees C promote FnBP-mediated biofilm development in meticillin-resistant Staphylococcus aureus clinical isolates.
abstract: The Staphylococcus aureus FnBPA and FnBPB proteins promote acid-induced biofilm accumulation. Meticillin-resistant S. aureus (MRSA) isolates from device-related infections with both fnbA and fnbB produced significantly more biofilm than isolates with either gene alone. Under mildly acidic growth conditions, FnBP-mediated biofilm and fnbA and fnbB transcript levels were substantially higher during growth at 37 degrees C than at 30 degrees C. Thus, in addition to a lowered pH, carriage of both fnbA and fnbB and growth at 37 degrees C promote MRSA biofilm development, further supporting a role for the FnBPA and FnBPB surface proteins in the pathogenesis of MRSA device-related infections.
authors.affiliation: Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland.|UCD School of Biomolecular and Biomedical Science, Ardmore House, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Eoghan
authors.initials: E
authors.lastname: O'Neill
authors.affiliation: Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Hilary
authors.initials: H
authors.lastname: Humphreys
authors.affiliation: UCD School of Biomolecular and Biomedical Science, Ardmore House, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: James P
authors.initials: JP
authors.lastname: O'Gara
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000502:physiology*; D016459:Prosthesis-Related Infections / Q000382:microbiology; D012189:Retrospective Studies; D013203:Staphylococcal Infections / Q000382:microbiology; D013696:Temperature
chemical_list: D018829:Adhesins, Bacterial; C078635:fibronectin-binding proteins, bacterial