Molecular cytogenetic analysis of urothelial carcinomas using urine samples.
Urinary cells obtained from voided urine specimens of 46 patients with urothelial carcinomas (UCs) and 10 normal individuals were analyzed with 3 different centromeric fluorescence in situ hybridization (FISH) probes. The overall sensitivity of cytology was 48.9% compared to 95.7%with the FISH technique. The minimum values were found for stage Ta and grade 1 (90.5 and 89.4) and sensitivity of FISH in other stages and grades was 100%. Chromosome 3 demonstrated the most frequent chromosomal abnormality in all samples (43%), followed by chromosome 17 (32%) and chromosome 7 (25%). There was a statistically significant association between the number of cell abnormalities in chromosome 17 and the tumour stage (p value=0.02). No relationship was found between the type of chromosomal abnormality and grade. Thus feasibility and reliability of a FISH based approach was confirmed for detection of UC in urine samples.
Ayati
M
M
Department of Urooncology, Imam Khomeini Hospital, Tehran, Iran.
Tousi
V Najjaran
VN
Sabokba
T
T
Vaziri-Nasab
H
H
Mehrkhani
F
F
Jamshidian
H
H
Dastan
J
J
Mosavi-Jarrahi
A R
AR
Ghaffari
S R
SR
D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000652:urine*; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D003581:Cytodiagnosis / Q000379:methods; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007492:Iran; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000652:urine*; D014556:Urine / Q000166:cytology*