title: Zwitterionic molecularly imprinted polymer-based solid-phase micro-extraction coupled with molecularly imprinted polymer sensor for ultra-trace sensing of L-histidine.
abstract: The proposed L-histidine sensing system composed of a molecularly imprinted solid-phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non-specific sorption and false-positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid-phase microextraction material. The hyphenated approach was able to detect L-histidine quantitatively with a limit of detection as low as 0.0435 ng/mL (RSD = 0.2%, S/N = 3).
authors.affiliation: Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India. prof.bbpd@yahoo.com
authors.forename: Bhim Bali
authors.initials: BB
authors.lastname: Prasad
authors.forename: Khushaboo
authors.initials: K
authors.lastname: Tiwari
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Singh
authors.forename: Piyush S
authors.initials: PS
authors.lastname: Sharma
authors.forename: Amit K
authors.initials: AK
authors.lastname: Patel
authors.forename: Shrinkhala
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D006639:Histidine / Q000032:analysis*; D007477:Ions / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods*
chemical_list: D007477:Ions; D011108:Polymers; D006639:Histidine