title: Biotin-tagged probes for MMP expression and activation: design, synthesis, and binding properties.
abstract: The design and synthesis of biotin chain-terminated inhibitors (BTI) showing high affinity for matrix metalloproteinases (MMPs) on one side and high affinity for avidin through the biotinylated tag on the other are reported. The affinity of the designed BTI toward five different MMPs has been evaluated and the simultaneous formation of a highly stable ternary system Avidin-BTI-MMP clearly assessed. This system will permit the development of new approaches to detect, quantify, or collect MMPs in biological samples, with potential applications in vivo.
authors.affiliation: Magnetic Resonance Center - University of Florence, Sesto Fiorentino, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Dragoni
authors.forename: Vito
authors.initials: V
authors.lastname: Calderone
authors.forename: Marco
authors.initials: M
authors.lastname: Fragai
authors.forename: Rahul
authors.initials: R
authors.lastname: Jaiswal
authors.forename: Claudio
authors.initials: C
authors.lastname: Luchinat
authors.forename: Cristina
authors.initials: C
authors.lastname: Nativi
mesh_terms: D001360:Avidin / Q000378:metabolism; D001710:Biotin / Q000378:metabolism*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D015195:Drug Design; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008958:Models, Molecular; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D004791:Enzyme Inhibitors; D015335:Molecular Probes; D001360:Avidin; D001710:Biotin; D045726:Metalloproteases