Overexpression of CHMP6 induces cellular oncosis and apoptosis in HeLa cells.
Cell death can proceed via at least two distinct pathways, apoptosis and oncosis. Apoptosis is an energy-dependent process characterized morphologically by cell shrinkage, whereas oncosis is defined as a prelethal pathway leading to cell death associated with cellular swelling, organelle swelling, and increased membrane permeability. In this study, we found that overexpression of chromatin modifying protein 6 (CHMP6) induced cell death by a series of experiments, including morphological observation, intracellular ATP determination, caspase-3 activity, and flow cytometry. Typical morphological characteristics consistent with oncosis were observed by transmission electron microscopy. Simultaneously, we obtained some results that indicated apoptosis, but the anti-apoptotic gene Bcl-xL and caspase family inhibitor Z-VAD-FMK had little effect on CHMP6-induced cell death. These results suggest that CHMP6 overexpression can cause cell death, predominantly via oncosis and to a certain extent via apoptosis, and that CHMP6 might be a novel regulator involved in both oncosis and apoptosis.
Fu
Dongxu
D
Laboratory of Medical Immunology, School of Basic Medical Science, Harbin Medical University, Harbin, PR China.
Tian
Linjie
L
Peng
Zhi
Z
Deng
Weiwei
W
Yuan
Jinsong
J
Ma
Dalong
D
Shi
Taiping
T
Li
Dianjun
D
Wang
Ying
Y
D000255:Adenosine Triphosphate / Q000172:deficiency; D017209:Apoptosis / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D056827:Endosomal Sorting Complexes Required for Transport; D004789:Enzyme Activation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D033921:Vesicular Transport Proteins / Q000235:genetics*
C481528:CHMP6 protein, human; D056827:Endosomal Sorting Complexes Required for Transport; D033921:Vesicular Transport Proteins; D000255:Adenosine Triphosphate; D020169:Caspases