Detection of cerebral metabolites in a canine model of ischemic stroke using 1H magnetic resonance spectroscopy.
Proton magnetic resonance spectroscopy ((1)H MRS) provides in vivo biochemical information on tissue metabolites. The purpose of this study was to investigate the serial metabolic changes of (1)H MRS in the cerebrum of ischemic dogs. An ischemic stroke was induced in five health laboratory beagle dogs by permanent middle cerebral artery occlusion using a silicone plug. (1)H MRS was serially performed three times with a 1.5-T MR system: before, three days after and 10days after the stroke. Immunohistochemical staining was performed to determine the expression of neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) at both the ipsilateral and contralateral cerebral cortex. Reduced levels of N-acetyl-asparate (p<0.05), choline (Cho), creatine (Cr) and myo-inositol (mI), and a marked increase in the lactate (Lac) level (p<0.01) were found at three days after the stroke. At 10days after the stroke, the levels of Lac significantly increased (p<0.01); however, the other metabolites were partially elevated. The changes of Cr, Cho and mI were not statistically significant (p>0.05) when the before and after stroke values were compared. There was a significant loss of NeuN and GFAP immunoreactivity at the ischemic core. (1)H MRS may be to a useful diagnostic tool for the evaluation of ischemic stroke in dogs.
Kang
Byeong-Teck
BT
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, #1 Hwayang-dong, Gwang-jin-gu, Seoul 143-701, South Korea.
Jang
Dong-Pyo
DP
Lee
Jong-Hwan
JH
Jung
Dong-In
DI
Gu
Su-Hyun
SH
Lim
Chae-Young
CY
Kim
Young-Bo
YB
Quan
Fu-Shi
FS
Kim
Hyung-Joong
HJ
Woo
Eung-Je
EJ
Cho
Zang-Hee
ZH
Park
Hee-Myung
HM
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000662:veterinary*; D002371:Cat Diseases / Q000473:pathology; D002415:Cats; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology / Q000662:veterinary; D008279:Magnetic Resonance Imaging / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D020521:Stroke / Q000473:pathology / Q000662:veterinary*