MlcR, a zinc cluster activator protein, is able to bind to a single (A/T)CGG site of cognate asymmetric motifs in the ML-236B (compactin) biosynthetic gene cluster.
All of the binding sequences for MlcR, a transcriptional activator of ML-236B (compactin) biosynthetic genes in Penicillium citrinum, were identified by an in vitro gel-shift assay. All the identified sequences contain an asymmetric direct repeat comprised of conserved tetrad bases (A/T)CGG with a spacer sequence of high similarity; in particular, G at position 2 and T at position 3 in the spacer are well conserved. The first (A/T)CGG repeat was essential for MlcR-binding and MlcR could bind to this monomeric site, probably as a monomer. This binding feature might enable MlcR to tolerate the variation of the spacer length and compositions in vitro. From these data, we propose that the consensus binding motif for MlcR is an asymmetric direct repeat, 5'-(A/T)CGG-NGTN(3-6)-TCGG-3'.
Baba
Satoshi
S
International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. satoshi_baba@icb.osaka-u.ac.jp
Kinoshita
Hiroshi
H
Hosobuchi
Masahiko
M
Nihira
Takuya
T
D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family; D009841:Oligonucleotides / Q000737:chemistry; D010407:Penicillium / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D015032:Zinc / Q000737:chemistry; D016335:Zinc Fingers / Q000235:genetics*
D001426:Bacterial Proteins; D009841:Oligonucleotides; D011994:Recombinant Proteins; D015534:Trans-Activators; C012258:mevastatin; D008148:Lovastatin; D015032:Zinc