A model system to study antimicrobial strategies in endodontic biofilms.
The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
Estrela
Carlos
C
Department of Oral Science, Federal University of Goiás, Goiânia, GO, Brazil. estrela3@terra.com.br
Sydney
Gilson Blitzkow
GB
Figueiredo
José Antonio Poli
JA
Estrela
Cyntia Rodrigues de Araújo
CR
D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003804:Dentin / Q000382:microbiology; D013293:Enterococcus faecalis / Q000254:growth & development*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry
D003470:Culture Media