Cultivation of Rhodobacter sphaeroides in the stirred bioreactor with different feeding strategies for CoQ(10) production.
The logistic growth model combined with the Luedeking-Piret equation was adopted in this study to model the batch production of CoQ(10) in the cultivation of Rhodobacter sphaeroides. The simulation results indicated that CoQ(10) production was a primary metabolite. As being a primary metabolite, a longer cell growing stage would tend to accumulate more biomass and lead to a higher CoQ(10) concentration being produced. In this context, a fed-batch operation by molasses feeding was performed to increase the biomass and subsequent CoQ(10) production. Three different molasses feeding strategies were operated in this study. Results suggested that the fed-batch operation with molasses controlled at 10 + or - 1 g/l could increase the cell mass and CoQ(10) concentration to reach their maximum values of 18.6 g/l and 83.8 mg/l, respectively, nearly 2.2 times and 1.9 times their respective values obtained in the batch cultivation.
Yen
Hong-Wei
HW
Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan, Republic of China. hwyen@thu.edu.tw
Feng
Chang-Yu
CY
Kang
Jia-Lin
JL
D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D003198:Computer Simulation; D005285:Fermentation; D016015:Logistic Models; D008965:Molasses; D012242:Rhodobacter sphaeroides / Q000254:growth & development*; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D014451:Ubiquinone; C024989:coenzyme Q10