Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli.
Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin beta subunit, resulting in overexpression of a fluorescent allophycocyanin beta-subunit in Escherichia coli. A high-density cell culture was developed using a continuous feeding strategy. After 16 h of culture, the dry cell density reached 21.4 g l(-1), the expression of the allophycocyanin beta-subunit was 0.86 g l(-1) broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein.
Ge
Baosheng
B
Center for Biotechnology and Bioengineering, China University of Petroleum, Qingdao, Shandong 266555, PR China. gbshx@yahoo.com.cn
Sun
Haixiang
H
Feng
Yang
Y
Yang
Jinying
J
Qin
Song
S
D018533:Biomass; D019149:Bioreactors; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D005822:Genetic Vectors; D008190:Lyases / Q000235:genetics / Q000378:metabolism; D010798:Phycocyanin / Q000096:biosynthesis*; D021122:Protein Subunits / Q000378:metabolism*
D021122:Protein Subunits; C002497:allophycocyanin; D010798:Phycocyanin; D008190:Lyases