PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores.
This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores.
Rawsthorne
H
H
Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, NC 27695-7624, USA. Helen_Rawsthorne@NCSU.edu
Dock
C N
CN
Jaykus
L A
LA
D001386:Azides / Q000378:metabolism / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism / Q000494:pharmacology*; D050296:Microbial Viability; D016133:Polymerase Chain Reaction / Q000379:methods*; D011419:Propidium / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013170:Spores / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D001386:Azides; D005456:Fluorescent Dyes; C533957:propidium monoazide; D011419:Propidium; D004247:DNA