Multiplexed DNA detection using spectrally encoded porous SiO2 photonic crystal particles.
A particle-based multiplexed DNA assay based on encoded porous SiO(2) photonic crystal disks is demonstrated. A "spectral barcode" is generated by electrochemical etch of a single-crystal silicon wafer using a programmed current-time waveform. A lithographic procedure is used to isolate cylindrical microparticles 25 microm in diameter and 10 microm thick, which are then oxidized, modified with a silane linker, and conjugated to various amino-functionalized oligonucleotide probes via cyanuric chloride. It is shown that the particles can be decoded based on their reflectivity spectra and that a multiple analyte assay can be performed in a single sample with a modified fluorescence microscope. The homogeneity of the reflectivity and fluorescence spectra, both within and across the microparticles, is also reported.
Meade
Shawn O
SO
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, USA.
Chen
Michelle Y
MY
Sailor
Michael J
MJ
Miskelly
Gordon M
GM
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004563:Electrochemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008027:Light; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D017785:Photons; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012821:Silanes; D012822:Silicon Dioxide; D004247:DNA