title: Aminobisphosphonate stimulates bone regeneration and enforces consolidation of titanium implant into a new rat caudal vertebrae model.
abstract: Bisphosphonates are widely used as therapeutic agents in bone disorders including cancer metastasis due to their osteoclast inhibitory effect. Recent data shows that bisphosphonates may also induce bone-building by stimulating osteoblast activity. Clinical observations, however, have revealed that bisphosphonates may cause necrosis in the oral cavity which questions their usefulness in bone regeneration during the consolidation of inorganic implants. Here we report the investigation of bone neogenesis following chronic amine bisphosphonate (Zometa) treatment in a novel experimental model, using the rat tail vertebra as a support. This method involves (1) implantation of titan screw into the tail vertebrae, (2) systemic bisphosphonate treatment and (3) quantitative biophysical measurements which mirrors consolidation of implant, i.e. strength of fixation and changes in newly formed bone architecture using micro Computer Tomograph (micro-CT). The degree of fixation of titan implants (osseointegration) increased by 36% on the effect of Zometa and the structure of newly formed bone became robust. The mass of new bone increased 3.1-fold at 6 weeks of regeneration, as compared to controls. Thus, Zometa, a potent aminobisphosphonate used in therapy of cancer metastases, osteoporosis and bone marrow transplantation, significantly increased bone neogenesis and enforced osseointegration of titan implants as measured quantitatively in the rat tail vertebra. Our data support the usefulness of aminobisphosphonates in the rehabilitation of bone loss as well as in improvement osseointegration of implants. We emphasise that this novel method may open up new possibilities for screening the effects of local and systemic treatments.
authors.affiliation: Department of Oral Biology, Semmelweis University, Nagyvárad tér 4., Budapest, 1089, Hungary. blajozs@net.sote.hu
authors.forename: József
authors.initials: J
authors.lastname: Blazsek
authors.forename: Csaba
authors.initials: C
authors.lastname: Dobó Nagy
authors.forename: István
authors.initials: I
authors.lastname: Blazsek
authors.forename: Rita
authors.initials: R
authors.lastname: Varga
authors.forename: Bálint
authors.initials: B
authors.lastname: Vecsei
authors.forename: Pál
authors.initials: P
authors.lastname: Fejérdy
authors.forename: Gábor
authors.initials: G
authors.lastname: Varga
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D001863:Bone Screws; D004164:Diphosphonates / Q000494:pharmacology*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D023421:Models, Animal; D019919:Prosthesis Implantation; D051381:Rats; D017208:Rats, Wistar; D013623:Tail / Q000000981:diagnostic imaging; D014025:Titanium; D014057:Tomography, X-Ray Computed; D014714:Vertebrates / Q000601:surgery*; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid; D014025:Titanium