Novel tryptamine-related substances, 5-sulphatoxydiacetyltryptamine, 5-hydroxydiacetyltryptamine, and reduced melatonin in human urine and the determination of those compounds, 6-sulphatoxymelatonin, and melatonin with fluorometric HPLC.
During our studies to establish a method for identifying tryptamine-related substances in human urine, we detected three large peaks of unknown origin in an HPLC chromatogram. Fluorometric HPLC and HPLC-TOF-MS/MS analyses led to the identification of these substances as 6-sulphatoxymelatonin, 5-sulphatoxydiacetyltryptamine, and reduced melatonin. This is the first report of the latter two compounds in human urine. Here, we report the results of two fluorometric HPLC assays of these three substances, as well as melatonin, 6-hydroxymelatonin, and 5-hydroxydiacetyltryptamine, using synthesized standards and discuss the possibility that 5-hydroxydiacetyltryptamine (the parent substance of 5-sulphatoxydiacetyltryptamine) and reduced melatonin have radical scavenging activity.
Minami
Masayasu
M
Chronic Fatigue Research Center, Institute for Science of Labour, 2-8-14 Sugao, Miyamae-ku, Kawasaki 216-8501, Japan.
Takahashi
Hideyo
H
Inagaki
Hirofumi
H
Yamano
Yuko
Y
Onoue
Sakura
S
Matsumoto
Shun
S
Sasaki
Tsukasa
T
Sakai
Kazuhiro
K
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry; D006801:Humans; D008297:Male; D008550:Melatonin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000652:urine; D014363:Tryptamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000652:urine; D055815:Young Adult
D014363:Tryptamines; D008550:Melatonin