Compatibility of hyaluronic acid hydrogel and skeletal muscle myoblasts.
Compatibility of hyaluronic acid hydrogel (HAH) and skeletal muscle myoblasts has been investigated for the first time in the present paper. Skeletal muscle myoblasts were separated from skeletons of rats and incubated with a HAH-containing culture medium. Cell morphology, hydrophilicity and cell adhesion of the HAH scaffold were investigated using optical microscopy, scanning electron microscopy, Hoechest33258 fluorescent staining, the immunocytochemistry method and water adsorption rate measurement. It was found that at a proper concentration (around 0.5%) of hyaluronic acid, the hydrogel possessed good compatibility with skeletal muscle myoblasts. The hydrogel can create a three-dimensional structure for the growth of skeletal muscle myoblasts and benefit cell attachment to provide a novel scaffold material for the tissue engineering of skeletal muscle.
Wang
Wei
W
Jinzhou Central Hospital, Jinzhou 121000, People's Republic of China. Weiwang Ly@yahoo.com.cn
Fan
Ming
M
Zhang
Li
L
Liu
Shu-hong
SH
Sun
Liang
L
Wang
Cheng-yue
CY
D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D006690:Bisbenzimidazole / Q000494:pharmacology; D049109:Cell Proliferation; D003470:Culture Media / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology*; D020100:Hydrogels / Q000737:chemistry*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects; D032446:Myoblasts / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D003470:Culture Media; D005456:Fluorescent Dyes; D020100:Hydrogels; D006820:Hyaluronic Acid; D006690:Bisbenzimidazole