title: Impact of radiotherapy and chemotherapy on biomarkers of oxidative DNA damage in lung cancer patients.
abstract: OBJECTIVE
  To assess oxidative damage to DNA during lung cancer (LC) treatments.
  
  
  METHODS
  Urinary levels of 8-oxoguanine (8-oxoGua) and levels of 8-oxo-2'-deoxyguanosine (8-oxodG) from urine and whole blood were determined in 36 non-cancer controls and 65 LC patients before any treatments. Samples were also obtained of LC patients during and after radiotherapy (RT, n=33) and chemotherapy (CT, n=16).
  
  
  RESULTS
  Stage IV LC patients had higher urinary 8-oxoGua and 8-oxodG levels than patients with stage I-III disease (p=0.044 and p=0.034, respectively). Urinary 8-oxodG levels increased during the first week of RT (p<0.001). Nuclear 8-oxodG increased during RT and 3 months after start of RT. Nuclear 8-oxodG levels also rose between the first two CT cycles (p=0.043), and urinary 8-oxodG levels during the sixth CT cycle (p=0.009).
  
  
  CONCLUSIONS
  Urinary DNA damage biomarker levels may be associated with LC stage. Both RT and CT increase the parameters of DNA oxidation.
authors.affiliation: Department of Oncology, University of Tampere, P.O. Box 607, FI-33101 Tampere, Finland. macro1@sci.fi
authors.forename: Marika
authors.initials: M
authors.lastname: Crohns
authors.forename: Seppo
authors.initials: S
authors.lastname: Saarelainen
authors.forename: Marina
authors.initials: M
authors.lastname: Erhola
authors.forename: Hannu
authors.initials: H
authors.lastname: Alho
authors.forename: Pirkko
authors.initials: P
authors.lastname: Kellokumpu-Lehtinen
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine*; D004249:DNA Damage; D003710:Demography; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000532:radiotherapy* / Q000652:urine; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D016019:Survival Analysis; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; C024829:8-hydroxyguanine; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine