Collaborative work on evaluation of ovarian toxicity by repeated-dose and fertility studies in female rats.
The National Institute of Health Sciences (NIHS) and 18 pharmaceutical companies of the Japan Pharmaceutical Manufacturers Association (JPMA) have conducted a validation study intended to evaluate whether a 2-week repeated general toxicity period with histopathological examination is sufficient to detect ovarian toxicity or not. The current repeated dose general toxicity study is considered to be insufficient in terms of evaluating female reproductive function due to a lack of evidence indicating that it is adequate. Evaluation of ovarian toxicity by comprehensive histopathological examination of the female reproductive organs based on the underlying morphology of a normal cycle of the reproductive tract including the ovary and additional immunohistochemical staining with proliferative cell nuclear antigen (PCNA) to identify small follicles may be a good tool to assess female reproductive function. In the collaborative study, 2- or 4-week repeated dose toxicity studies with ovarian histopathological examinations were conducted. A female fertility study was also conducted to compare the results with those of the ovarian histopathological findings. A total of 17 test substances were evaluated and categorized into hormone analogues, primordial follicle damaging agents, metabolite imbalance inducers, and endocrine imbalance inducers. Based on the results, ovarian toxicity could be detected by a careful histopatholgical examination. A 2-week dosing period may be sufficient for the evaluation of ovarian toxicity, except for cytotoxic compounds such as alkylating agents. The pathological findings of ovarian toxicity (decreases in follicles, increases in atretic follicles, increases in currently formed corpora lutea, etc) reflected the female fertility parameters (irregular estrous cycle, pre-implantation loss).
Sanbuissho
Atsushi
A
Medicinal Safety Research Laboratories, R&D Division, DAIICHI SANKYO CO., LTD., Fukuroi, Shizuoka, Japan. sanbuissho.atsushi.tv@dasiichisankyo.co.jp
Yoshida
Midori
M
Hisada
Shigeru
S
Sagami
Fumio
F
Kudo
Satoshi
S
Kumazawa
Toshihiko
T
Ube
Masayuki
M
Komatsu
Shinichi
S
Ohno
Yasuo
Y
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007564:Japan; D010049:Ovarian Diseases / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D055818:Public-Private Sector Partnerships; D051381:Rats; D011919:Rats, Inbred Strains; D012958:Societies, Scientific; D018675:Toxicity Tests / Q000379:methods*; D015262:Xenobiotics / Q000633:toxicity*
D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D015262:Xenobiotics