[Effects of experimental liver injury on the intestinal barrier in rats.].
OBJECTIVE
To study the effects of experimental liver injury on the intestinal barrier, and to evaluate the significance of plasma D(-)-lactate, diamine oxidase (DAO) and endotoxin in live injury.


METHODS
Fifty-five rats were randomized into the acute liver failure group (group C, n = 25), acute liver injury group (group B, n = 15), and control group (group A, n = 15). The concentrations of D(-)-lactate, DAO and endotoxin in plasma were detected by spectrophotograph. The morphology and subcellular structure were observed under optical microscope and transmission electron microscope.


RESULTS
Acute liver failure and acute liver injury models were established successfully. The concentrations of D(-)-lactate and DAO in the plasma of experimental groups (group B and C) were significantly higher than those in the control group (P less than 0.05); the concentration of intestinal DAO in experimental groups were significantly lower than that in the control group (P less than 0.05); the level of endotoxin in C group was significantly higher than that in group A and group B (P less than 0.05).


CONCLUSIONS
Liver injury induces hyperpermeability of the rat intestinal mucosal barrier, plasma D(-)-lactate and DAO are sensitive markers for early diagnosis of liver injury, plasma endotoxin may accelerate deterioration of liver function.
Yao
Ying
Y
Department of Center for Liver Disease, Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China.
Xu
Zhi-Yuan
ZY
Chen
Xue-Ping
XP
Shen
Li-Juan
LJ
Gao
Jian-Peng
JP
You
Li-Ying
LY
Yang
Jin-Hui
JH
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D007413:Intestinal Mucosa; D007422:Intestines; D008111:Liver Function Tests; D051381:Rats; D017208:Rats, Wistar
D006631:Amine Oxidase (Copper-Containing)