title: Establishment and characterization of a fibroblast cell line from the Mongolian horse.
abstract: A fibroblast line was successfully established from Mongolian horse ear marginal tissue by using a primary explant technique and cell cryogenic preservation technology. Biological analysis showed the following: The cells were adherent and exhibited density-dependent inhibition of proliferation; assays of microbial contamination from bacteria, fungi, and mycoplasma were negative; the population doubling time of the cells was 33.9 h; and a 2n chromosome number of 64 at a frequency higher than 80%. A lack of cross-contamination of this cell line with other species was confirmed by isoenzyme analysis of lactic and malic dehydrogenases. In order to study exogenous gene expression, four fluorescent proteins, pEGFP-N3, pEGFP-C1, pDsRed1-N1, and pEYFP-N1, were transfected into the cells. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 12 h after transfection. This cell line not only preserves the genetic resources of the Mongolian horse at the cellular level but also provides valuable materials for genomic, postgenomic, and somacloning research in this species.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. lilinfeng219@126.com
authors.forename: Lin-feng
authors.initials: LF
authors.lastname: Li
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Guan
authors.forename: Yue
authors.initials: Y
authors.lastname: Hua
authors.forename: Xiu-juan
authors.initials: XJ
authors.lastname: Bai
authors.forename: Yue-hui
authors.initials: YH
authors.lastname: Ma
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D015925:Cryopreservation; D005347:Fibroblasts / Q000166:cytology*; D006736:Horses / Q000235:genetics*; D007621:Karyotyping; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D008164:Luminescent Proteins