[Effect of bisphenol A on the structure of organs and the expression of some cytokines in rats].
OBJECTIVE
To study effect of bisphenol A on the structure of organs and the expression of some cytokines of spleens in F344 rats.


METHODS
Fisher 344 rats were divided into control, low-dose, middle-dose and high-dose groups, and were gavaged to 4 mg/kg, 40 mg/kg, 400 mg/kg bisphenol A per day, respectively. To observe the change of histomorphology of spleens, thymus, kidneys and livers. To detect the mRNA expression of IL-2, IL-12, IFN-gamma, TNF-alpha in spleen and Fas, FasL in thymus by real-time RT-PCR.


RESULTS
Contrast to control, the weight of high dose group decreased (P < 0.05). In 400 mg/kg group, white pulp of spleens reduced, splenic corpuscle contracted, granular degeneration was visible in hepatocyte, and tubular atrophy was occurred in some kidneys of rats. But there were not any change in others groups. The mRNA expression of IL-2, IL-12, IFN-gamma, TNF-alpha in all groups decreased significantly (P < 0.01). The expression of Fas decrease in all treatment groups but the expression of FasL up-regulated in middle and high dose groups after it decrease in low dose group (P < 0.05).


CONCLUSIONS
The environmental endocrine disruptor such as bisphenol A has the same chemical structure as hormone, they can change the development and the function of target organs, possible influence the expression of some cytokines and the immune function.
Miao
Song
S
Shandong Medical College, Jinan 250002, China.
Kou
Zengqiang
Z
Gao
Zhixian
Z
D000818:Animals; D001559:Benzhydryl Compounds; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D013950:Thymus Gland / Q000378:metabolism / Q000473:pathology; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D016207:Cytokines; D052244:Endocrine Disruptors; D007371:Interferon-gamma; D018664:Interleukin-12; D007376:Interleukin-2; D010636:Phenols; D012333:RNA, Messenger; C006780:bisphenol A; D000098826:Bisphenol A Compounds