title: Analysis of gene sets based on the underlying regulatory network.
abstract: Networks are often used to represent the interactions among genes and proteins. These interactions are known to play an important role in vital cell functions and should be included in the analysis of genes that are differentially expressed. Methods of gene set analysis take advantage of external biological information and analyze a priori defined sets of genes. These methods can potentially preserve the correlation among genes; however, they do not directly incorporate the information about the gene network. In this paper, we propose a latent variable model that directly incorporates the network information. We then use the theory of mixed linear models to present a general inference framework for the problem of testing the significance of subnetworks. Several possible test procedures are introduced and a network based method for testing the changes in expression levels of genes as well as the structure of the network is presented. The performance of the proposed method is compared with methods of gene set analysis using both simulation studies, as well as real data on genes related to the galactose utilization pathway in yeast.
authors.affiliation: Department of Statistics, University of Michigan, Ann Arbor, Michigan 48109, USA. shojaie@umich.edu
authors.forename: Ali
authors.initials: A
authors.lastname: Shojaie
authors.forename: George
authors.initials: G
authors.lastname: Michailidis
mesh_terms: D003198:Computer Simulation; D005690:Galactose / Q000378:metabolism; D053263:Gene Regulatory Networks; D005800:Genes, Fungal; D008957:Models, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D005690:Galactose