DNA-binding and cleavage studies of a novel two-dimensional manganese(II) azide complex with N-methylimidazole.
A new complex, manganese(II) azide complex with N-methylimidazole, has been synthesized and characterized by elemental analysis, IR spectra and single crystal X-ray studies. Absorption spectroscopy, emission spectroscopy, viscosity measurements and CD spectroscopy have been used to investigate the binding of the complex with calf thymus DNA (CTDNA). The intrinsic binding constant K(b) of the complex with DNA was obtained by electronic absorption titration; the value is consistent with the result by fluorescence titration method. The spectroscopic studies together with viscosity measurements support the claim that the title complex bonds to CT-DNA by a groove mode. Control cleavage experiments using pBR 322 plasmid DNA which suggest minor groove binding for the complex.
Chen
Fengjuan
F
State Key Laboratory of Applied Organic Chemistry, and College of Chemistry and Chemical Engineering, Lanzhou University, PR China.
Xu
Zhihong
Z
Xi
Pinxian
P
Liu
Xiaohui
X
Zeng
Zhengzhi
Z
D000818:Animals; D001386:Azides / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D004269:DNA, Bacterial / Q000737:chemistry*; D004563:Electrochemistry; D005453:Fluorescence; D007093:Imidazoles / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010957:Plasmids; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D014783:Viscosity
D001386:Azides; D004269:DNA, Bacterial; D007093:Imidazoles; D009942:Organometallic Compounds; D008345:Manganese; D004247:DNA; C427203:calf thymus DNA; C018100:1-methylimidazole