Identification of autoantibody against beta-amyloid peptide in the serum of elderly.
Alzheimer's disease (AD) is characterized by two major neurological features: amyloid deposits and neurofibrillary tangles in the brain. According to the amyloid cascade hypothesis, accumulation of amyloid-beta peptide (A-beta) plays a central role in the pathogenesis of AD. Several lines of evidence suggest that antibodies against A-beta play a protective role in the neuropathology of AD. In this study, we describe the purification of an autoantibody against A-beta from human serum using affinity purification method. The purified autoantibody recognized A-beta deposits in the brain of aged Tg2676 mice, an animal model of AD. The serum levels of anti-A-beta autoantibody correlated inversely with age in both AD patients and control non-demented elderly subjects. Furthermore, the levels were significantly lower in AD patients compared with the age-matched control subjects. It is the first time to show the identification of endogenous anti-A-beta autoantibody in human serum and suggesting that serum levels of anti-A-beta autoantibody might be a good biomarker for AD patients.
Sohn
Ji-Hoon
JH
University College of Medicine, Seoul, 110-799, Korea.
So
Jung On
JO
Hong
Hyun Joo
HJ
Kim
Jong Won
JW
Na
Duk Ryul
DR
Kim
Manho
M
Kim
Hee
H
Nam
Eunjoo
E
Ha
Hee Jin
HJ
Kim
Young Ho
YH
Mook-Jung
Inhee
I
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016229:Amyloid beta-Peptides / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008875:Middle Aged
D016229:Amyloid beta-Peptides; D001323:Autoantibodies