Systemic first-line phenotyping.
With the completion of the mouse genome sequence an essential task for biomedical sciences in the twenty-first century will be the generation and functional analysis of mouse models for every gene in the mammalian genome. More than 30,000 mutations in ES cells will be engineered and thousands of mouse disease models will become available over the coming years by the collaborative effort of the International Mouse Knockout Consortium. In order to realize the full value of the mouse models proper characterization, archiving and dissemination of mouse disease models to the research community have to be performed. Phenotyping centers (mouse clinics) provide the necessary capacity, broad expertise, equipment, and infrastructure to carry out large-scale systemic first-line phenotyping. Using the example of the German Mouse Clinic (GMC) we will introduce the reader to the different aspects of the organization of a mouse clinic and present selected methods used in first-line phenotyping.
Gailus-Durner
Valérie
V
Institute of Experimental Genetics, Helmholtz Zentrum München, Neuherberg, Germany.
Fuchs
Helmut
H
Adler
Thure
T
Aguilar Pimentel
Antonio
A
Becker
Lore
L
Bolle
Ines
I
Calzada-Wack
Julia
J
Dalke
Claudia
C
Ehrhardt
Nicole
N
Ferwagner
Barbara
B
Hans
Wolfgang
W
Hölter
Sabine M
SM
Hölzlwimmer
Gabriele
G
Horsch
Marion
M
Javaheri
Anahita
A
Kallnik
Magdalena
M
Kling
Eva
E
Lengger
Christoph
C
Mörth
Corinna
C
Mossbrugger
Ilona
I
Naton
Beatrix
B
Prehn
Cornelia
C
Puk
Oliver
O
Rathkolb
Birgit
B
Rozman
Jan
J
Schrewe
Anja
A
Thiele
Frank
F
Adamski
Jerzy
J
Aigner
Bernhard
B
Behrendt
Heidrun
H
Busch
Dirk H
DH
Favor
Jack
J
Graw
Jochen
J
Heldmaier
Gerhard
G
Ivandic
Boris
B
Katus
Hugo
H
Klingenspor
Martin
M
Klopstock
Thomas
T
Kremmer
Elisabeth
E
Ollert
Markus
M
Quintanilla-Martinez
Leticia
L
Schulz
Holger
H
Wolf
Eckhard
E
Wurst
Wolfgang
W
de Angelis
Martin Hrabé
MH
D000818:Animals; D003628:Database Management Systems; D016247:Information Storage and Retrieval / Q000379:methods*; D051379:Mice; D010641:Phenotype