Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing.
The therapeutic use of proteins has created an increasing demand for feasible and economical methods for both up- and downstream processes. However, whereas upstream processes have attracted substantial investment and commercial attention, downstream processing has been overlooked, causing a production bottleneck that is shifting the costs of production. This review focuses on the use of aqueous two-phase extraction as an option for the downstream processing of therapeutic proteins. It is a potential and promising liquid-liquid extraction technique for the purification of biomolecules, such as monoclonal antibodies, growth factors and hormones, that combines a high selectivity and biocompatibility with an easy scale-up and continuous operation mode.
Azevedo
Ana M
AM
Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
Rosa
Paula A J
PA
Ferreira
I Filipa
IF
Aires-Barros
M Raquel
MR
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001702:Biopharmaceutics / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D019382:Human Growth Hormone / Q000302:isolation & purification; D006801:Humans; D007328:Insulin / Q000302:isolation & purification; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry
D000911:Antibodies, Monoclonal; D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; D004364:Pharmaceutical Preparations; D011108:Polymers; D019382:Human Growth Hormone