Click-chemistry-conjugated oligo-angiomax in the two-dimensional DNA lattice and its interaction with thrombin.
The recently arising antithrombin drug, angiomax, was successfully conjugated with a 5'-amino oligonucleotide through click chemistry. This oligo-angiomax conjugate was assembled into a two-dimensional DNA lattice with other oligonucleotides together. Besides the plane sheet of DNA lattices, an interesting angiomax-involved DNA tubing structure, constructed by 40 to 50 angiomax stripes which are parallel to the longitudinal axis of the tube, was also imaged. After incubation of thrombins with the angiomax-involved DNA lattice, the binding of thrombins to arrayed angiomax peptides was observed. Finally a chromogenic substrate bioassay was employed to estimate the antithrombin activities as assembled oligo-angiomax DNA lattice approximately 1.1, oligo-angiomax approximately 2.7 angiomax. The functionalized DNA lattices have the potential to be used as a powerful platform for investigation of biomolecular interactions such as drug-protein, protein-protein, DNA-RNA, and DNA-protein interactions in the nano- and subnanoscales.
Chao
Jie
J
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, Jiangsu, People's Republic of China.
Huang
Wen-Yi
WY
Wang
Jing
J
Xiao
Shou-Jun
SJ
Tang
Yan-Chun
YC
Liu
Jian-Ning
JN
D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism; D000991:Antithrombins / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006629:Hirudins / Q000737:chemistry* / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013816:Thermodynamics; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism*
D000925:Anticoagulants; D000991:Antithrombins; D006629:Hirudins; D009841:Oligonucleotides; D009842:Oligopeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; C014413:chromozym TH; D004247:DNA; D013917:Thrombin; C074619:bivalirudin