title: Lipid hydroperoxides in human plasma after ethanol consumption.
abstract: Oxidative stress contributes to the pathogenesis of alcoholic liver disease. The purpose of this study is to estimate the amount of oxidative stress that is present when healthy humans consume moderate amounts of ethanol. Blood was collected from healthy volunteers before, 1 h, and 3 h after drinking 400 ml of Japanese rice wine at the rate of 100 ml per 5 min. The aldehyde dehydrogenase 2 genotype and the concentrations of blood ethanol, total lipid hydroperoxides (LOOH), and cholesterol hydroperoxides were determined. The plasma LOOH was found to have significantly increased 1h after drinking. Cholesterol hydroperoxides were not detected in plasma, either before or after drinking. There was no relationship between the LOOH and the ethanol concentration. We showed that one-shot of moderate ethanol consumption temporarily increases the plasma LOOH in healthy volunteers but excessive plasma LOOH compounds were eliminated within a short time.
authors.affiliation: Division of Legal Medicine, Department of Social Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. asano@med.kobe-u.ac.jp
authors.forename: Migiwa
authors.initials: M
authors.lastname: Asano
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nushida
authors.forename: Junko
authors.initials: J
authors.lastname: Adachi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nagasaki
authors.forename: Kanako
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Azumi
authors.initials: A
authors.lastname: Kuse
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000097:blood*; D000444:Aldehyde Dehydrogenase / Q000235:genetics; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D002492:Central Nervous System Depressants / Q000097:blood; D002784:Cholesterol / Q000031:analogs & derivatives / Q000097:blood; D000431:Ethanol / Q000097:blood; D005260:Female; D053593:Forensic Toxicology; D005838:Genotype; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male
chemical_list: D002492:Central Nervous System Depressants; D008054:Lipid Peroxides; D000431:Ethanol; C002843:cholesterol hydroperoxide; D002784:Cholesterol; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial