[Determination of pesticide residues from seed coating reagent in agricultural products using ultra performance liquid chromatography-tandem mass spectrometry].
An ultra performance liquid chromatography-tandem mass spectrometric method (UPLC-MS/MS) has been developed for the simultaneous determination of eight pesticide residues from seed coating in fruits, vegetable and grain. The sample was extracted by methanol-water (1:1, v/v) and determined by ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry in positive mode (ESI+) and multiple reaction monitoring (MRM) mode. The UPLC analyses were performed on an Acquity UPLC C18 column with gradient eluation. The utility of the method was demonstrated by the analysis of crude extracts, with no sample clean up, from soybean. The linear range was 1 - 200 microg/L. The correlation coefficients (r) were under 0.997. The average recoveries of eight pesticides in samples (from 0.006 to 1.2 mg/kg) ranged from 60% to 110%, and the relative standard deviations (RSDs) were less than 10%. The results indicate that the method is easier, faster, more sensitive, and suitable for the qualitative and quantitative confirmation of pesticide residues from seed coating reagent in fruit, vegetable and grain samples.
Chen
Yue
Y
School of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Wang
Jinhua
J
Lu
Xiaoyu
X
Wang
Wanchun
W
Huang
Mei
M
Xu
Chaoyi
C
D053000:Analytic Sample Preparation Methods; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D012639:Seeds; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D007202:Indicators and Reagents; D010573:Pesticide Residues