title: Regulation of episomal gene expression by KRAB/KAP1-mediated histone modifications.
abstract: KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studied within the environment of chromosomal DNA. Here we demonstrate that KRAB/KAP1 regulation is fully functional within the context of episomal DNA, such as adeno-associated viral and nonintegrated lentiviral vectors, and is correlated with histone modifications typically associated with this epigenetic regulator.
authors.affiliation: School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Barde
authors.forename: Elisa
authors.initials: E
authors.lastname: Laurenti
authors.forename: Sonia
authors.initials: S
authors.lastname: Verp
authors.forename: Anna Claire
authors.initials: AC
authors.lastname: Groner
authors.forename: Christopher
authors.initials: C
authors.lastname: Towne
authors.forename: Viviane
authors.initials: V
authors.lastname: Padrun
authors.forename: Patrick
authors.initials: P
authors.lastname: Aebischer
authors.forename: Andreas
authors.initials: A
authors.lastname: Trumpp
authors.forename: Didier
authors.initials: D
authors.lastname: Trono
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006657:Histones / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D000076183:Tripartite Motif-Containing Protein 28
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D012097:Repressor Proteins; C422407:ZNF350 protein, human; D004247:DNA; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28