Novel models of cancer-related anemia in mice inoculated with IL-6-producing tumor cells.
We established models of cancer-related anemia in mice from subcutaneous inoculation of two IL-6-producing cancer cell lines, human lung cancer cell line LC-06-JCK and murine colon26 clone 5 colon cancer cells. In both models, elevated levels of IL-6 were detected in sera and hemoglobin levels significantly decreased compared with non-tumor-bearing mice. In the LC-06-JCK model, serum albumin levels also decreased with elevated levels of human IL-6 in sera. On the other hand, serum levels of EPO increased, although anemia developed and did not improve. The development of cancer-related anemia was prevented by the administration of a rat anti-mouse IL-6 receptor antibody, MR16-1, in the LC-06-JCK model. It is therefore suggested that IL-6 causes anemia independent of a reduction in EPO levels. Our preclinical models should be useful for exploring new modalities for the treatment of cancer-related anemia.
Mori
Kazushige
K
Department of Product Research, Chugai Pharmaceutical Co., Ltd., Kamakura, Japan. morikzs@chugai-pharm.co.jp
Fujimoto-Ouchi
Kaori
K
Onuma
Etsuro
E
Noguchi
Mariko
M
Shimonaka
Yasushi
Y
Yasuno
Hideyuki
H
Nishimura
Takashi
T
D000740:Anemia / Q000209:etiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats
D000911:Antibodies, Monoclonal; D016207:Cytokines; D015850:Interleukin-6; D004921:Erythropoietin