title: Two-dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus.
abstract: The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immunosuppressed patients. We established a 2-D reference map for A. fumigatus. Using MALDI-TOF-MS/MS, we identified 381 spots representing 334 proteins. Proteins involved in cellular metabolism, protein synthesis, transport processes and cell cycle were most abundant. Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified.
authors.affiliation: Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knoell-Institute (HKI), Jena, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Vödisch
authors.forename: Daniela
authors.initials: D
authors.lastname: Albrecht
authors.forename: Franziska
authors.initials: F
authors.lastname: Lessing
authors.forename: André D
authors.initials: AD
authors.lastname: Schmidt
authors.forename: Robert
authors.initials: R
authors.lastname: Winkler
authors.forename: Reinhard
authors.initials: R
authors.lastname: Guthke
authors.forename: Axel A
authors.initials: AA
authors.lastname: Brakhage
authors.forename: Olaf
authors.initials: O
authors.lastname: Kniemeyer
mesh_terms: D001232:Aspergillus fumigatus / Q000166:cytology / Q000378:metabolism*; D002453:Cell Cycle; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D014176:Protein Biosynthesis; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins; D020543:Proteome