title: Regulation of environmental factors on the expression of a solid-state specific lipase (Lip1) with Rhizopus chinensis by western blot and indirect Elisa.
abstract: To identify which solid-state typical environmental factors are involved in the induction of a solid-state special lipase (Lip1), western blot and Elisa based on Lip1 antibody were used. A low water activity played a significant role in the induction of Lip1, as evidenced by the increased expression level (20-46 microg/g dry cell) along with the decrease of water activity (0.927-0.969). Physical barrier against hyphal extension was found to be another required factor, since the expression of Lip1 was significantly enhanced by 3-fold using a membrane with smaller pore size (0.45 and 0.22 microm) covered on top of surface culture.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Shu Yang
authors.initials: SY
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D004777:Environment; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005246:Feedback / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D008049:Lipase / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology*
chemical_list: D008049:Lipase