START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region.
START-GAP2, also termed as DLC2, is a START domain-containing RhoGAP and a negative regulator of RhoA and Cdc42. Although it was reported as a tumor suppresser gene product, the molecular basis for function of START-GAP2 remains to be clarified. Here, we demonstrate that START-GAP2 is localized in focal adhesions through a "FAT (focal adhesion targeting)" region in the N-terminal half. START-GAP2 competes with START-GAP1/DLC1, another START domain-containing RhoGAP, in focal adhesion targeting. Moreover, the C-terminus of tensin2, one of focal adhesion components and reported to bind START-GAP1, also directly interacts with START-GAP2. These results suggest that START-GAP2 and START-GAP1 share the same molecular mechanism in targeting to focal adhesions.
Kawai
Katsuhisa
K
Graduate School of Life Science, University of Hyogo, Harima Science Garden City, Hyogo-ken 978-1297, Japan.
Seike
Jun-ichi
J
Iino
Takuya
T
Kiyota
Minoru
M
Iwamae
Yui
Y
Nishitani
Hideo
H
Yagisawa
Hitoshi
H
D000818:Animals; D022001:Focal Adhesions / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D000070876:Tensins; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D008840:Microfilament Proteins; D010744:Phosphoric Monoester Hydrolases; D000070876:Tensins; D025521:Tumor Suppressor Proteins; C473606:Stard13 protein, mouse; C452816:TNS2 protein, human