[Study on activity and mechanism of Sini Decoction anti-mitochondrial oxidation injury caused by myocardial ischemia/reperfusion].
OBJECTIVE
To study the activity and mechanism of Sini Decoction (SND) Anti-mitochondrial Oxidation Injury caused by Myocardial Ischemia/Reperfusion.


METHODS
Kun ming mice were randomly divided into three groups: Control group, Ischemia/Reperfusion (I/R) group and SND-treated group. At the end of experiment,hearts of mice were taken out for further detection. Activity of myocardium and mitochondrial SOD, content of myocardium and mitochondrial MDA, swelling of mitochondria, Lactic Acid content of myocardium and MnSODmRNA expression were observed.


RESULTS
SND could increase the activity of myocardium and mitochondrial SOD (P<0.01), decrease the content of myocardium and mitochondrial MDA (P<0.01), decrease the Lactic Acid content of myocardium, lighted the swelling of mitochondria (P<0.01) and change the expression of MnSODmRNA (P<0.01).


CONCLUSIONS
Sini decoction can anti-mitochondrial oxidation injury caused by Myocardial Ischemia/Reperfusion, its mechanism may be relate to increasing the MnSODmRNA expression.
Zhao
Dan-Yang
DY
Guangzhou Children's Hospital, Guangzhou 510120, China.
Zhao
Ming-Qi
MQ
Wu
Wei-Kang
WK
D031841:Aconitum / Q000737:chemistry; D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D020939:Zingiber officinale / Q000737:chemistry; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D008315:Malondialdehyde; D013482:Superoxide Dismutase