title: Development of an autofluorescent organophosphates-degrading Stenotrophomonas sp. with dehalogenase activity for the biodegradation of hexachlorocyclohexane (HCH).
abstract: Simultaneous biodegradation of hexachlorocyclohexane (HCH) and organophosphates (OPs) by a recombinant Stenotrophomonas sp. was studied in the study. The broad-host-range plasmid pVGAB, harboring enhanced green fluorescent protein gene (egfp) and dehalogenase genes (linA and linB), was constructed and transformed into the OP-degrading strain Stenotrophomonas YC-1 to get the recombinant strain YC-H. Over-expression of dehalogenase (LinA and LinB) and enhanced green fluorescent protein (EGFP) was obtained in YC-H by determining their enzymatic activities and fluorescence intensity. YC-H was capable of rapidly and simultaneously degrading 10mg/l gamma-HCH and 100mg/l methyl parathion (MP) determined by GC-ECD analysis. A bioremediation assay with YC-H inoculated into fumigated and nonfumigated soil showed that both 10mg/kg gamma-HCH and 100mg/kg MP could be completely degraded within 32 days. The novel EGFP-marked bacterium could be potentially applied in the field-scale decontamination of HCH and OPs residues in the environment.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhao
authors.forename: Chuanling
authors.initials: C
authors.lastname: Qiao
mesh_terms: D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000737:chemistry* / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008743:Methyl Parathion / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry; D012988:Soil Microbiology; D020588:Stenotrophomonas / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D010755:Organophosphates; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D008743:Methyl Parathion; D001556:Hexachlorocyclohexane; D010088:Oxidoreductases