title: HPV genotypes in women with squamous intraepithelial lesions and normal cervixes participating in a community-based microbicide study in Pretoria, South Africa.
abstract: BACKGROUND
  Little is known regarding the human papillomaviruses (HPV) genotypes prevalent in women in South Africa, a country with a high incidence of cervical cancer.
  
  
  OBJECTIVE
  To determine the prevalence and HPV genotypes in women with squamous abnormalities and normal cervixes participating in a community-based microbicide study.
  
  
  METHODS
  A total of 159 cervical specimens, including 56 specimens from women with abnormal cytology (cases) and 103 randomly selected specimens from women with normal cytology (controls), were collected. HPV was detected by consensus PCR primers and HPV genotypes were determined by Roche Linear Array HPV genotyping assay.
  
  
  RESULTS
  HPV genotypes were found in 91% of cases and 40% of controls (p<0.005). High-risk HPV was detected in all high-grade squamous intraepithelial lesions (HSILs), 69% of low-grade squamous intraepithelial lesions (LSILs), 57% of atypical squamous cells of undetermined significance (ASCUS), and 86% of ASCUS in which HSIL could not be excluded (ASCUS-H), and 73% of HPV positive controls. HPV-35 was the predominant genotype in HSILs; HPV-18 in ASCUS; HPV-58 in ASCUS-H and HPV-16 in LSILs and controls.
  
  
  CONCLUSIONS
  High-risk HPV prevalence was high in both cases and controls. HPV genotype distribution in HSILs was different from that reported worldwide and from other studies in South Africa.
authors.affiliation: Faculty of Health Science, Department of Medical Microbiology, University of Pretoria, South Africa. ahlammdd@yahoo.com
authors.forename: H M
authors.initials: HM
authors.lastname: Said
authors.forename: K
authors.initials: K
authors.lastname: Ahmed
authors.forename: R
authors.initials: R
authors.lastname: Burnett
authors.forename: B R
authors.initials: BR
authors.lastname: Allan
authors.forename: A-L
authors.initials: AL
authors.lastname: Williamson
authors.forename: A A
authors.initials: AA
authors.lastname: Hoosen
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use; D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D018307:Neoplasms, Squamous Cell / Q000150:complications*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D013019:South Africa / Q000453:epidemiology
chemical_list: D000890:Anti-Infective Agents