title: Use of ion mobility spectroscopy with an ultraviolet ionization source as a vanguard screening system for the detection and determination of acetone in urine as a biomarker for cow and human diseases.
abstract: An ion mobility spectrometer equipped with an ultraviolet lamp was used for the qualitative and quantitative determination of acetone in urine samples. This analyte can be used as a biomarker for some fat metabolism-related diseases in humans and cows. Samples require no pretreatment other than warming at 80 degrees C for 5 min, after which an N(2) stream is used to drive volatile analytes to the ion mobility spectrometer. The precision of the ensuing method, expressed as relative standard deviation (%RSD), is better in all cases than 6.7% for peak height and calculated at three levels of concentration. The analyte concentration range studied was from 5 to 80 mg L(-1), its limit of detection in the aqueous matrix 3 mg L(-1) and recoveries from spiked urine samples 109+/-3%. The calculated reduced mobility for acetone in the urine samples, 1.75+/-0.04 cm(2)V(-1)s(-1), was similar to previously reported values. Also, the results were consistent with those provided by test strips used for reference. The proposed method provides a new vanguard screening system for determining acetone in urine samples.
authors.affiliation: Department of Analytical Chemistry, University of Córdoba, Annex C3 Building, Campus of Rabanales, E-14071 Córdoba, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Garrido-Delgado
authors.forename: L
authors.initials: L
authors.lastname: Arce
authors.forename: C C
authors.initials: CC
authors.lastname: Pérez-Marín
authors.forename: M
authors.initials: M
authors.lastname: Valcárcel
mesh_terms: D000096:Acetone / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D003933:Diagnosis; D006801:Humans; D008659:Metabolic Diseases / Q000175:diagnosis* / Q000662:veterinary; D015203:Reproducibility of Results; D013057:Spectrum Analysis; D014466:Ultraviolet Rays
chemical_list: D015415:Biomarkers; D000096:Acetone