title: Simultaneous determination of polyphenolic compounds in red and white grapes grown in Sardinia by high performance liquid chromatography-electron spray ionisation-mass spectrometry.
abstract: A new method for the identification and the quantification of nonanthocyanin phenolic compounds from six Vitis Vinifera grape varieties native to Sardinia (three native: Vermentino, Malvasia and Cannonau and three non-native types: Chardonnay, Sauvignon and Cabernet Sauvignon; Argiolas vineyard) was developed. This rapid and selective method employs LC/ESI-MS in negative mode. Different solvents extraction and different sorbents for purification were compared to the direct analysis of the initial extracts without further sample preparation. A total of 54 phenolic compounds were identified either in the freeze-dried skins or seeds, including nonflavonoids (hydroxybenzoic and hydroxycinnamic acids and their derivatives, stilbenes) and flavonoids (flavanols, flavonols, dihydroxyflavonols).
authors.affiliation: Saras Ricerche e Tecnologie Spa, Assemini, CA, Italy.
authors.forename: Laurent
authors.initials: L
authors.lastname: Hollecker
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pinna
authors.forename: Giorgia
authors.initials: G
authors.lastname: Filippino
authors.forename: Stefania
authors.initials: S
authors.lastname: Scrugli
authors.forename: Barbara
authors.initials: B
authors.lastname: Pinna
authors.forename: Francesca
authors.initials: F
authors.lastname: Argiolas
authors.forename: Mariano
authors.initials: M
authors.lastname: Murru
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D004355:Drug Stability; D005419:Flavonoids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000032:analysis*; D007558:Italy; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
chemical_list: D003373:Coumaric Acids; D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols