title: Hormonal regulation of GnRH and LHbeta mRNA expression in cultured rat granulosa cells.
abstract: We have recently demonstrated that the rat ovary expresses LHbeta, FSHbeta, and the common alpha subunit mRNA. In the present report, we studied the regulation of LHbeta and of gonadotropin-releasing hormone (GnRH) mRNA expression in granulosa cells that were isolated from immature rats treated with either estrogen or pregnant mare serum gonadotropin (PMSG). In both cell types, GnRH agonist treatment resulted in a decrease in LHbeta mRNA expression. However, only in cells derived from PMSG-treated rats, GnRH treatment produced an increase in GnRH mRNA expression. A markedly increased GnRH mRNA expression was also obtained in granulosa cells derived from PMSG-primed rats in response to LH. In addition, FSH reduced the expression of LHbeta mRNA in granulosa cells from estrogen-primed rats. These results suggest that the expression of LHbeta in the ovary is regulated by locally produced GnRH and by FSH from either the ovary or the pituitary.
authors.affiliation: Department of Neurobiology, Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Naomi
authors.initials: N
authors.lastname: Litichever
authors.forename: Eran
authors.initials: E
authors.lastname: Gershon
authors.forename: Nava
authors.initials: N
authors.lastname: Dekel
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Koch
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D005786:Gene Expression Regulation; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology*; D006736:Horses; D006801:Humans; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012756:Sheep
chemical_list: D037101:Luteinizing Hormone, beta Subunit; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone