title: [The effect of ischemic postconditioning on apoptosis induced by acute hot renal ischemic-reperfusion].
abstract: OBJECTIVE
  To investigate the effects of ischemic postconditioning(IPo) on cellular apoptosis in acute renal ischemic-reperfusion injury (IRI) in rats.
  
  
  METHODS
  Model of acute renal ischemic-reperfusion injury (IRI) was constructed using Wistar rats. Forty healthy male rats were divided into 4 groups randomly,including group C,group IRI, group IPo and group Ado. Rats of group C received right nephronectomy and separation of left renal vessels. Before reperfusion, group IPo was perfused for 10 s, following ischemic reperfusion for another 10 s with 6 cycles. Group Ado was infused with adenosine intravenously 10 min before ischemia. Cellular apoptosis was determined with TUNEL (TdT-mediated dUTP Nick End Labelling), flowcytometry and histopathological staining.
  
  
  RESULTS
  Compared with group C, the apoptotic index increased predominatly in group IRI (P<0.05), whereas, apoptosis index decreased obviousely in group IPo, Ado (P<0.05). In addition, the apoptosis index between group IPo and Ado was not statistically different.
  
  
  CONCLUSIONS
  Ischemic postconditioning could decrease cellular apoptosis induced by IRI in rat kidney.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yu-chun
authors.initials: YC
authors.lastname: Zhu
authors.forename: Tie-long
authors.initials: TL
authors.lastname: Tang
authors.forename: Shu
authors.initials: S
authors.lastname: Cui
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Guo-jun
authors.initials: GJ
authors.lastname: Zeng
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply* / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*