Isolation of a sulphated polysaccharide from a recently discovered sponge species (Celtodoryx girardae) and determination of its anti-herpetic activity.
Exopolysaccharides (EPS) were extracted from a sponge, Celtodoryx girardae recently discovered in the Golfe du Morbihan in 2000. Sponge samples were collected monthly from November 2007 to May 2008. SEC analysis of EPS samples showed that they exhibit a unique molecular weight of approximately 800 kDa. However, infrared analysis revealed that structural seasonal variations occur. EPS fractions also exhibit significant sulphate contents and were screened in vitro for a potential antiviral activity against Herpes simplex virus type 1 (HSV-1). The best result was obtained with a sample collected in January which exhibits an EC(50) of 5.9 microg/mL without cytotoxicity on the Vero cell line. Experiments carried out to elucidate the mechanism of the EPS showed that the sulphated groups of EPS interact with the glycoproteins on the surface of the virus' membrane.
Rashid
Zalilawati Mat
ZM
LIMAT B, Université Européenne de Bretagne, Vannes, France.
Lahaye
Elodie
E
Defer
Diane
D
Douzenel
Philippe
P
Perrin
Bertrand
B
Bourgougnon
Nathalie
N
Sire
Olivier
O
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D002850:Chromatography, Gel; D004353:Drug Evaluation, Preclinical; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D016018:Least-Squares Analysis; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry*; D025341:Principal Component Analysis; D013055:Spectrophotometry, Infrared; D014709:Vero Cells
D000998:Antiviral Agents; D011134:Polysaccharides