Oxaloacetate decreases the infarct size and attenuates the reduction in evoked responses after photothrombotic focal ischemia in the rat cortex.
A traumatic brain injury or a focal brain lesion is followed by acute excitotoxicity caused by the presence of abnormally high glutamate (Glu) levels in the cerebrospinal and interstitial fluids. It has recently been demonstrated that this excess Glu in the brain can be eliminated into the blood following the intravenous administration of oxaloacetate (OxAc), which, by scavenging the blood Glu, induces an enhanced and neuroprotective brain-to-blood Glu efflux. In this study, we subjected rats to a photothrombotic lesion and treated them after the illumination with a single 30-min-long administration of OxAc (1.2 mg/100 g, i.v.). Following induction of the lesion, we measured the infarct size and the amplitudes of the somatosensory evoked potentials (SEPs) as recorded from the skull surface. The photothrombotic lesion resulted in appreciably decreased amplitudes of the evoked potentials, but OxAc administration significantly attenuated this reduction, and also the infarct size assessed histologically. We suggest that the neuroprotective effects of OxAc are due to its blood Glu-scavenging activity, which, by increasing the brain-to-blood Glu efflux, reduces the excess Glu responsible for the anatomical and functional correlates of the ischemia, as evaluated by electrophysiological evoked potential (EP) measurements.
Nagy
David
D
Department of Physiology, Anatomy and Neuroscience, University of Szeged, 6726 Szeged, Hungary. david.nagy01@gmail.com
Marosi
Mate
M
Kis
Zsolt
Z
Farkas
Tamas
T
Rakos
Gabriella
G
Vecsei
Laszlo
L
Teichberg
Vivian I
VI
Toldi
Jozsef
J
D000818:Animals; D002544:Cerebral Infarction / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects / Q000502:physiology*; D005452:Fluoresceins; D008297:Male; D009930:Organic Chemicals; D062907:Oxaloacetic Acid / Q000627:therapeutic use*; D051381:Rats; D012395:Rose Bengal / Q000633:toxicity; D013003:Somatosensory Cortex / Q000098:blood supply / Q000473:pathology* / Q000503:physiopathology*
D005452:Fluoresceins; D009930:Organic Chemicals; C435731:fluoro jade; D012395:Rose Bengal; D062907:Oxaloacetic Acid