Metabolic imaging of the organ of corti--a window on cochlea bioenergetics.
Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.
Tiede
LeAnn
L
Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA.
Steyger
Peter S
PS
Nichols
Michael G
MG
Hallworth
Richard
R
D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D005839:Gentamicins / Q000494:pharmacology; D018069:Hair Cells, Vestibular / Q000378:metabolism / Q000648:ultrastructure; D066298:In Vitro Techniques; D051379:Mice; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D009243:NAD / Q000378:metabolism*; D009925:Organ of Corti / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D019265:Spectroscopy, Near-Infrared / Q000379:methods
D000617:Aminoglycosides; D005839:Gentamicins; D009243:NAD