title: Reticulated platelet levels in whole blood and platelet-rich plasma of dogs with various platelet counts measured by flow cytometry.
abstract: Reticulated platelets (RP) are young platelets that contain residual RNA, and measurement of RP has been to assess thrombopoiesis. In the present study, flow cytometric counts of RP were compared using paired specimens elicited from dogs with various platelet counts by different RP collection procedures, the whole blood method (WBM) and platelet rich plasma method (PRPM). The flow cytometric counts of RP for the specimens collected by WBM showed good and stable agreement with those taken by PRPM from the same canine subjects. The result revealed that WBM, as well as PRPM, can be used clinically to determine RP levels in dogs with abnormal platelet counts.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan. maruyama.haruhiko@nihon-u.ac.jp
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Maruyama
authors.forename: Hirozumi
authors.initials: H
authors.lastname: Yamagami
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D001792:Blood Platelets / Q000166:cytology* / Q000276:immunology; D004285:Dogs / Q000097:blood*; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D010976:Platelet Count / Q000379:methods / Q000662:veterinary*; D053657:Platelet-Rich Plasma / Q000166:cytology*; D011804:Quinolines; D012313:RNA / Q000032:analysis; D034061:Thrombopoiesis / Q000502:physiology
chemical_list: D052160:Benzothiazoles; D011804:Quinolines; C051000:thiazole orange; D012313:RNA