Mitochondrial compartment: a possible target of cadmium effects on breast epithelial cells.
Cadmium-breast epithelial cell interactions were studied by analyzing some mitochondria-related aspects of stress response. We treated immortalized non-tumor breast cells with 5 or 50 microM CdCl(2) for 24 or 96 h demonstrating that the exposure did not cause a significant mitochondrial proliferation, while it induced a significant increase in the respiratory activity and mitochondrial polarization. In addition, we found that hsp60 was up-regulated while hsp70 and COXII and COXIV were down-regulated. The mRNA for hsp70 remained constant and only the inducible form of the 70-kDa heat shock protein was over expressed. The mRNAs for COXII and COXIV remained constant after 24 h and increased after longer incubations while the respective proteins decreased. These findings provide additional information on the cellular and molecular aspects of the interaction between Cd and epithelial cells, and on alterations of mitochondria as early events in Cd cytotoxicity.
Cannino
Giuseppe
G
Dipartimento di Biologia Cellulare e dello Sviluppo "A.Monroy", Università di Palermo, Italy.
Ferruggia
Elisa
E
Luparello
Claudio
C
Rinaldi
Anna Maria
AM
D001940:Breast / Q000166:cytology*; D002104:Cadmium / Q000633:toxicity*; D002460:Cell Line; D019069:Cell Respiration / Q000187:drug effects; D018834:Chaperonin 60 / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D003576:Electron Transport Complex IV / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008566:Membranes; D008928:Mitochondria / Q000187:drug effects*
D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; D003576:Electron Transport Complex IV