title: A Simple Method for Determining Aphelenchoides besseyi Infestation Level of Oryza sativa Seeds.
abstract: A simple extraction method was developed for obtaining the white tip nematode, Aphelenchoides besseyi Christie, from single seeds of rice, Oryza sativa. The method was as follows: Individual rice seeds were split longitudinally and then transferred into single pipet tips. Tips containing a split seed were then singly placed upright in glass vials with water to extract the nematodes. This method was more efficient than the Baermann funnel technique and allowed nearly 100% recovery of living A. besseyi from single rice seeds within 4 hours.
authors.forename: S
authors.initials: S
authors.lastname: Hoshino
authors.forename: K
authors.initials: K
authors.lastname: Togashi