title: [Researches on cloning and expression of the gene encoding Schistosoma japonicum tetraspanins extracellular loop 2 and its immunogenicity].
abstract: OBJECTIVE
  To clone and express the gene (Sj-tsp-2) encoding extracellular loop 2 (EC-2) of tetraspanins (TSPs) of Schistosoma japonicum (Chinese strain), and then to study the antigenicity and immunogenicity of this protein.
  
  
  METHODS
  Synthesized Sj-tsp-2 gene was cloned into prokaryotic expression vector pET32a to generate pET32a-Sj-tsp-2 recombinant plasmid, and transformed into competent E. coli BL21 (DE3). After inducing with IPTG, the expressed fusion protein was purified under nondenaturing conditions.
  
  
  RESULTS
  The recombinant was confirmed by sequence analysis. The size of fusion protein and interest peptide was accords with the theoretical value by SDS-PAGE analysis. Additionally, the results of Western Blotting and ELISA demonstrated that the expressed protein had good immunogenicity. More importantly, we confirmed that TSP-2 is mainly located on the surface of S. japonicum.
  
  
  CONCLUSIONS
  The successful expression and purification of recombinant protein Trx-Sj-TSP-2 will be very helpful for the further study of its protection role in animals.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yi-jie
authors.initials: YJ
authors.lastname: Fu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.forename: Chuang
authors.initials: C
authors.lastname: Yuan
authors.forename: Xian-cheng
authors.initials: XC
authors.lastname: Chen
authors.forename: Yu-quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins