title: Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula.
abstract: Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Almeida
authors.forename: N F
authors.initials: NF
authors.lastname: Azevedo
authors.forename: C
authors.initials: C
authors.lastname: Iversen
authors.forename: S
authors.initials: S
authors.lastname: Fanning
authors.forename: C W
authors.initials: CW
authors.lastname: Keevil
authors.forename: M J
authors.initials: MJ
authors.lastname: Vieira
mesh_terms: D044083:Cronobacter sakazakii / Q000302:isolation & purification*; D005516:Food Microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D041943:Infant Formula; D015345:Oligonucleotide Probes / Q000235:genetics*; D020135:Peptide Nucleic Acids; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids