Multilamellar liposomes of triamcinolone acetonide: preparation, stability, and characterization.
The aim of this study was to assess and characterize the stability of multilamellar liposomes as a delivery vehicle for triamcinolone acetonide. A standardized preparation method for a liposomal delivery vehicle was developed, after varying composition and storage conditions, and assessing encapsulation efficiency and loss of active principle. The assessment of temperature as a factor in formula stability during storage showed that stability improved under refrigeration (4-6 degrees C) (less early diffusion of active principle through the liposomal wall), in comparison with samples stored at room temperature. To improve stability, cholesterol was added to some formulae, which although resulting in a decrease in average encapsulation efficiency, mitigated subsequent losses of retained active principle (formulae 4, 5, and 6), in comparison with those without cholesterol (formulae 1, 2, and 3). This was evident both under refrigerated and room-temperature conditions. Finally, after testing the effects of adding an antioxidant and/or preservative to the formulae, a liposomal design was achieved with acceptable stability, vesicle dimensions, and encapsulation efficiency.
Clares
B
B
Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Granada, Spain.
Gallardo
V
V
Medina
M M
MM
Ruiz
Ma A
MA
D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004355:Drug Stability; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D010316:Particle Size; D013696:Temperature; D014222:Triamcinolone Acetonide / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004337:Drug Carriers; D008081:Liposomes; D002784:Cholesterol; D014222:Triamcinolone Acetonide