New anti-Ku80 monoclonal antibody F10H2.B3 as a useful marker for dividing cells in culture.
We report on the development of a mouse monoclonal antibody (named F10H2.B3) using the native cellular fragments of human fetal neural stem cells as immunogens. Molecular analysis has shown that the target antigen of F10H2.B3 is Ku80 (ATP-dependent DNA helicase 2 subunit 2 [EC 3.6.1.-]). We suggest this antibody could be used in certain conditions as a proliferation marker for cells of different origin.
Mikelsaar
Aavo-Valdur
AV
Institute of General and Molecular Pathology, Tartu University, Tartu, Estonia. aavo-valdur.mikelsaar@ut.ee
Sünter
Alar
A
Toomik
Peeter
P
Karpson
Kalmer
K
Juronen
Erkki
E
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D034961:Antigens, Nuclear / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002455:Cell Division / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D000072200:Ku Autoantigen; D051379:Mice; D008969:Molecular Sequence Data; D013234:Stem Cells / Q000276:immunology
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; C000606509:Xrcc6 protein, human; C000606510:Xrcc6 protein, mouse; D000072200:Ku Autoantigen