[Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC].
OBJECTIVE
To develop an HPLC method for the determination of eleutheroside B in As1 (the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue).


METHODS
The antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid (10: 90: 0.01). The UV detection wavelength was set at 344 nm.


RESULTS
As1 showed an excellent antifatigue activity; One compound was isolated from As1 and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0.104-20.8 microg (r = 0.9999), the average recovery was 97.68%, RSD 1.4% (n=6).


CONCLUSIONS
This HPLC method is simple,accurate and reliable.
Li
Chen
C
Shenyang Pharmaceutical University, shenyang 110016, China.
Wang
Xiao-yan
XY
Hu
Xu-wei
XW
Fang
Hai-tao
HT
Qiao
Shan-yi
SY
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D028001:Eleutherococcus / Q000737:chemistry*; D005221:Fatigue / Q000188:drug therapy* / Q000503:physiopathology; D005960:Glucosides / Q000032:analysis*; D006801:Humans; D008297:Male; D051379:Mice; D010666:Phenylpropionates / Q000032:analysis*; D013550:Swimming
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010666:Phenylpropionates; C028305:syringin