Pyridoxal-5'-phosphate phosphatase/chronophin inhibits long-term potentiation induction in the rat dentate gyrus.
Pyridoxal-5'-phosphate (PLP)-phosphatase/chronophin (PLPP/CIN) directly dephosphorylates actin-depolymerizing factor (ADF)/cofilin as well as PLP. Although PLPP/CIN plays a role in the regulation of F-actin and vitamin B(6) metabolism, there is no direct evidence to support a correlation between PLPP/CIN and F-actin polymerization during long-term potentiation (LTP) induction. In this study, we investigated whether the expression of PLPP/CIN is altered following LTP induction, and whether Tat-PLPP/CIN transduction affects LTP induction in the rat dentate gyrus (DG). PLPP/CIN immunoreactivity was markedly decreased in dentate granule cells after the induction of LTP. Tat-PLPP/CIN transduction (20 and 200 microg/kg) decreased the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude as compared to saline or Tat-protein-treated animals. The PLPP/CIN protein level showed an inverse correlation with phosphorylated ADF/cofilin levels and F-actin content. These findings suggest that PLPP/CIN-mediated actin dynamics may play an important role in the changes of morphological properties (dendritic spine reorganization) of the hippocampus in LTP.
Kim
Ji-Eun
JE
Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon-Do, South Korea.
Kim
Dae-Won
DW
Kwak
Sung-Eun
SE
Ryu
Hea Jin
HJ
Yeo
Seong-Il
SI
Kwon
Oh-Shin
OS
Choi
Soo-Young
SY
Kang
Tae-Cheon
TC
D051339:Actin Depolymerizing Factors / Q000378:metabolism; D000818:Animals; D001703:Biophysics; D018891:Dentate Gyrus / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D016014:Linear Models; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009479:Neuropeptides / Q000378:metabolism; D010590:Phalloidine / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D051381:Rats; D014161:Transduction, Genetic / Q000379:methods
D051339:Actin Depolymerizing Factors; D009479:Neuropeptides; C053386:drebrins; D010590:Phalloidine; C022493:pyridoxal-5-phosphatase; D010744:Phosphoric Monoester Hydrolases