title: Ly49P recognition of cytomegalovirus-infected cells expressing H2-Dk and CMV-encoded m04 correlates with the NK cell antiviral response.
abstract: Natural killer (NK) cells are crucial in resistance to certain viral infections, but the mechanisms used to recognize infected cells remain largely unknown. Here, we show that the activating Ly49P receptor recognizes cells infected with mouse cytomegalovirus (MCMV) by a process that requires the presence of H2-D(k) and the MCMV m04 protein. Using H2 chimeras between H2-D(b) and -D(k), we demonstrate that the H2-D(k) peptide-binding platform is required for Ly49P recognition. We identified m04 as a viral component necessary for recognition using a panel of MCMV-deletion mutant viruses and complementation of m04-deletion mutant (Deltam04) virus infection. MA/My mice, which express Ly49P and H2-D(k), are resistant to MCMV; however, infection with Deltam04 MCMV abrogates resistance. Depletion of NK cells in MA/My mice abrogates their resistance to wild-type MCMV infection, but does not significantly affect viral titers in mice infected with Deltam04 virus, implicating NK cells in host protection through m04-dependent recognition. These findings reveal a novel mechanism of major histocompatibility complex class I-restricted recognition of virally infected cells by an activating NK cell receptor.
authors.affiliation: Department of Human Genetics, McGill University, Montreal, Quebec, H3A 1B1, Canada.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kielczewska
authors.forename: Michal
authors.initials: M
authors.lastname: Pyzik
authors.forename: Tianhe
authors.initials: T
authors.lastname: Sun
authors.forename: Astrid
authors.initials: A
authors.lastname: Krmpotic
authors.forename: Melissa B
authors.initials: MB
authors.lastname: Lodoen
authors.forename: Michael W
authors.initials: MW
authors.lastname: Munks
authors.forename: Marina
authors.initials: M
authors.lastname: Babic
authors.forename: Ann B
authors.initials: AB
authors.lastname: Hill
authors.forename: Ulrich H
authors.initials: UH
authors.lastname: Koszinowski
authors.forename: Stipan
authors.initials: S
authors.lastname: Jonjic
authors.forename: Lewis L
authors.initials: LL
authors.lastname: Lanier
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Vidal
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000276:immunology*; D001681:Biological Assay; D002352:Carrier Proteins / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000821:virology*; D008213:Lymphocyte Activation; D051379:Mice; D018146:Muromegalovirus / Q000276:immunology*; D009154:Mutation / Q000235:genetics; D041681:NIH 3T3 Cells; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000998:Antiviral Agents; D002352:Carrier Proteins; D006023:Glycoproteins; D015395:Histocompatibility Antigens Class I; C400050:Ly49P receptor; D011971:Receptors, Immunologic; D014764:Viral Proteins; C104832:gp34 protein, cytomegalovirus