SK66-his, a novel glycine-rich peptide derived from Drosophila with antibacterial activity.
SK(66)-his, a novel glycine-rich peptide derived from the CG13551 gene of Drosophila, was directly expressed in Escherichia coli with the help of the glucose effect of the lac repressor and efficiently purified in high yield (10.063 mg/l). It showed significant activity against Gram-positive bacteria. We determined a MIC value of 9 microg ml(-1) with Bacillus thuringiensis.
Feng
Zhiguo
Z
Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Liu
Huijuan
H
Lang
Jun
J
Li
Yanjiao
Y
Shu
Muya
M
Chen
Zhengwang
Z
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004330:Drosophila / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005998:Glycine; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D010455:Peptides; D005998:Glycine