Allele frequency of CODIS 13 in Indonesian population.
Since the first application of DNA technology in 1985 in forensic cases, and the acceptance of this technology in 1988 at court, the DNA typing is widely used in personal identification, parentage cases and tracing the source of biological samples found in the crime scene. The FBI on 1990 had recommended the forensic labs to used 13 loci of Short Tandem Repeats (STR), known as CODIS 13, as the loci of choice for forensic use. The research on the population DNA database on these loci is extremely important for calculating the Paternity Index as well as Matching Probability for forensic application of DNA technology. As many as 402 unrelated persons, consisted of 322 from western part of Indonesia and 80 from eastern part of Indonesia, were chosen as the respondents of this research, after signing the informed consent. The peripheral blood sample was taken using sterile lancets and dropped onto FTA classic cards. The DNA was extracted by FTA purification solution (3x) and TE(-1) (2x), and amplified by PCR mix, either Cofiler or Profiler Plus (Perkin Elmers), followed by sequencing using ABI Prism type 3100 Avant Genetic Analyzer. The analysis showed that the alleles frequencies of Indonesian is specific, different with the other Asian populations with some specific alleles and microvariant were found.
Untoro
Evi
E
Department of Forensic Medicine and Medico-legal, Faculty of Medicine, University of Indonesia, Jl. Salemba Raya no. 6, Jakarta 10430, Indonesia. evi_untoro@yahoo.com
Atmadja
Djaja Surya
DS
Pu
Chang-En
CE
Wu
Fang-Chi
FC
D016172:DNA Fingerprinting; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D007214:Indonesia; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences