Open tubular layer of S-ofloxacin imprinted polymer fabricated in silica capillary for chiral CEC separation.
An open tubular molecule imprinted polymer (OT-MIP) capillary column has been prepared for chiral separation of ofloxacin enantiomers in CEC. The S-ofloxacin imprinted OT column was fabricated by thermally initiated non-covalent polymerization procedure inside a pretreated and silanized fused silica capillary. The template molecule was incorporated with methacrylic acid (MAA), ethylene glycol dimethacrylate (EDMA) and 4-styrenesulfonic acid (4-SSA) and dissolved in a porogen mixture of ACN/2-propanol (9:1). The separation efficiency of the 4-SSA MIP column was found quite better than that of the MIP column without 4-SSA. It has been demonstrated that our OT-MIP column can separate ofloxacin enantiomers with excellent chiral separation efficiency after tuning the various chromatographic conditions. The optimized chromatographic eluent was 85:15, v/v%, ACN/60 mM sodium acetate at pH 7. The separation efficiency and selectivity of chiral separation of this study were far better than those obtained by previous methods for chiral separation of R- and S-ofloxacin.
Zaidi
Shabi Abbas
SA
Department of Chemistry, Nano Fine Center, and Institute of Basic Research, Inha University, Incheon, South Korea.
Han
Kyoung Moon
KM
Kim
Sung Soon
SS
Hwang
Dong Guk
DG
Cheong
Won Jo
WJ
D002021:Buffers; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D015394:Molecular Structure; D015242:Ofloxacin / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D013343:Styrenes; D013451:Sulfonic Acids
D002021:Buffers; D011108:Polymers; D013343:Styrenes; D013451:Sulfonic Acids; D012822:Silicon Dioxide; D015242:Ofloxacin