[Isolation and identification of a Pp-LIM only A gene expressed during cold acclimation in Physcomitrella patens].
Cold acclimation can improve freezing tolerance. Here cDNA amplified fragment length polymorphism (cDNA-AFLP) was used to isolate differentially expressed cDNAs and a Pp-LIM only A cDNA was isolated and identified in the cold acclimation of Physcomitrella patens. Real-time RT-PCR indicated it is obviously up-regulated at 6 h, 12 h, 24 h, 48 h and 72 h after cold acclimation. After comparing the cDNA with the gene sequence, seven introns and eight exons were identified in the cDNA. The cDNA putatively encodes a protein of 345 amino acid residues and only contains one LIM domain which has highly similarity with the PDZ/LIM domains of the protein family in animals. We proposed that Pp-LIM only A be a new gene coding for the LIM-domain containing protein and enhance stability of cell membrane via their effects on cytoskeleton during cold acclimation in P. patens.
Sun
Mingming
M
College of Elementary Education, Capital Normal University, Beijing 100080, China.
Ma
Rongcai
R
He
Yikun
Y
D000064:Acclimatization / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D019068:Bryopsida / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D014157:Transcription Factors