title: Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro.
abstract: Pseudomonas fluorescens GcM5-1A, isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, was cultured in Luria Broth medium (LB). The clarified culture was extracted with ethyl acetate, and two dipeptides were purified from the extract. The chemical structures of 1 and 2 were identified as cyclo(-Pro-Val-)and cyclo(-Pro-Tyr-), respectively, by MS, (1)H NMR, (13)C NMR,(1)H-(1)H COSY, 1H -(13)C COSY spectra. Bioassay results showed that the two compounds were toxic to both suspension cells and seedlings of Pinus thunbergii, which may offer some clues to research the mechanism of pine wilt disease caused by PWN.
authors.affiliation: Department of Biology, Qingdao University, Qingdao 266071, P. R. China Department of Forest Entomology and Pathology, Nanjing Forest University, Nanjing 210037, P. R. China.
authors.forename: Qunqun
authors.initials: Q
authors.lastname: Guo
authors.forename: Daosen
authors.initials: D
authors.lastname: Guo
authors.forename: Boguang
authors.initials: B
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Ronggui
authors.initials: R
authors.lastname: Li