Curcumin-induced DNA damage and inhibited DNA repair genes expressions in mouse-rat hybrid retina ganglion cells (N18).
Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse-rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 microM of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 microM of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 microM of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3sigma, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.
Lu
Hsu-Feng
HF
Department of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan, ROC.
Yang
Jai-Sing
JS
Lai
Kuang-Chi
KC
Hsu
Shu-Chun
SC
Hsueh
Shu-Ching
SC
Chen
Yuan-Liang
YL
Chiang
Jo-Hua
JH
Lu
Chi-Cheng
CC
Lo
Chyi
C
Yang
Mei-Due
MD
Chung
Jing-Gung
JG
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D020552:Comet Assay; D003474:Curcumin / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D005347:Fibroblasts; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051379:Mice; D051381:Rats; D012165:Retinal Ganglion Cells / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003474:Curcumin