Sub-proteome analysis in the green flagellate alga Chlamydomonas reinhardtii.
In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical purification procedures of sub-cellular fractions. Combined with the available genome sequences, this paved the way to perform analysis of specific sub-proteomes by mass spectrometry. In this review, several approaches that provided comprehensive lists of components of certain sub-cellular compartments and their biological relevance will be described. These include proteins of chloroplast ribosomes, of flagella, of the eyespot as well as posttranslational and environmentally modified sub-proteomes. The power of such proteome approaches lies in the identification of novel components and modifications of a given sub-proteome that have not been discovered before. Information is usually gained at a large scale and is very valuable to further understand biological processes of a given cellular sub-compartment. But clearly the arduous task has then to be performed to further analyze the function of specific proteins/genes by RNA interference technology, mutant analyses or methods for identifying the protein interaction network within a sub-proteome.
Wagner
Volker
V
Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Boesger
Jens
J
Mittag
Maria
M
D000818:Animals; D002104:Cadmium / Q000378:metabolism; D002502:Centrioles / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D005407:Flagella / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D010750:Phosphoproteins / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D012269:Ribosomal Proteins / Q000378:metabolism
D010750:Phosphoproteins; D020543:Proteome; D012269:Ribosomal Proteins; D002104:Cadmium