[The effect of PKC activation and Smac release on inhibition of myocardial cell apoptosis by Sini Decoction].
OBJECTIVE
To investigate the effect of Sini Decoction on myocardial cell apoptosis after MI/R and the possible relation to myocardial cell apoptosis, PKC and Smac.


METHODS
Sprage-Dawley rats were divided into four groups: control group, sham group, MI/R group and SND group. The rats in MI/R group, left anterior descending coronary artery (LAD) was occluded for 1 h and reperfused for 1 h. The rats in SND group were pretreated with Sini decoction(5 g/kg x d) for three days, the last treatment was pretreated 24 h before the index occlusion. Cell apoptosis was measured by flow cytometry, the expression of PKC was measured by immunohistochemistry. The expression of Smac was analyzed by Western blotting. The activity of Caspase-3 was also measured.


RESULTS
24 h after Sini Decoction treatment, Myocardial cell apoptosis significantly decreased, PKC was translocated and its expression increased, the release of Smac decreased, the activity of Caspase-3 decreased.


CONCLUSIONS
Sini Decoction treatment can protect myocardial cell from apoptosis caused by MI/R, the mechanism is related to PKC activation, Mitochondria protection and in turn inhibition of Smac release, and inhibition of apoptosis signal transduction.
Liu
Ying
Y
Department of Pathophysiology of Guangdong Pharmacological College, Guangzhou 510006, China.
Nie
Yong-Mei
YM
Wu
Wei-Kang
WK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D007150:Immunohistochemistry; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D024101:Mitochondrial Proteins; D011493:Protein Kinase C; D053148:Caspase 3