High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling.
Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.
Karlsson
Hasse
H
Department of Medical Biochemistry and Cell Biology, Göteborg University, Gothenburg, Sweden.
Larsson
Jessica M Holmén
JM
Thomsson
Kristina A
KA
Härd
Iris
I
Bäckström
Malin
M
Hansson
Gunnar C
GC
D015153:Blotting, Western / Q000379:methods; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D054794:Glycomics / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D009077:Mucins; D011134:Polysaccharides