title: Acetylcholine influences growth cone motility and morphology of developing thalamic axons.
abstract: The neurotransmitter acetylcholine (ACh) is expressed in the developing telencephalon at the time when thalamic axons project to the cortex, long before synapses are being formed. Since previous studies demonstrated an influence of ACh on neurite extension we used different in vitro assays to examine possible effects of ACh on the growth of thalamic axons. In explant cultures, application of ACh reduced the length of thalamic axons in a dose dependent manner, an effect that could also be evoked by selective muscarinic and nicotinic agonists. Time-lapse imaging of thalamic axons exposed to microscopic gradients of ACh revealed that growth cones no longer advanced, but maintained high filopodial activity. This growth cone pausing was not accompanied by axon retraction or growth cone collapse. It could at least partially be blocked by muscarinic and nicotinic antagonists, indicating that both types of ACh receptors contribute to mediate these effects on thalamic axons. Finally, we also found that ACh changed the morphology of growth cones; they became larger and extended more filopodia. Since such changes in the structure and motility of growth cones are observed at decision regions along the path of many fiber populations including thalamic axons, we suggest that ACh plays a role during the elaboration of thalamocortical projections.
authors.affiliation: Universität Jena, Institut für Allgemeine Zoologie und Tierphysiologie, Jena, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Rüdiger
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bolz
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000187:drug effects* / Q000378:metabolism; D020439:Growth Cones / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011976:Receptors, Muscarinic / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism; D013788:Thalamus / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D000109:Acetylcholine