[Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome].
OBJECTIVE
To establish a flow microbeads assay (FMA) to examine the level of hemorrhagic fever with renal syndrome virus (HFRS V.) specific IgM, IgG and cytokines in HFRS patients.


METHODS
Serum samples from 28 cases of HFRS and 20 healthy controls were studied. Serum levels of anti-HFRS V. antibodies were qualified and inflammatory cytokines IL-6 and TNF-alpha were quantified by FMA. The results were compared with the results by ELISA.


RESULTS
FMA showed that the positivity rates for anti-HFRS V. IgM and IgG were 92.85% and 71.43%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (532.62+/-397.19) ng/L and (392.68+/-177.68)ng/L, respectively, which were significantly higher than that in healthy control group (38.77+/-20.32 ng/L and 15.91+/-6.91 ng/L, P<0.01). In contrast, ELISA showed that the positivity rates for anti-HFRS V. IgM and IgG were 71.43% and 50.00%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (256.46+/-102.51) ng/L ang (45.63+/-5.32) ng/L, respectively, which were significantly higher than that in healthy control group (53.80+/-19.21 ng/L and 5.81+/-3.58 ng/L, P<0.01).


CONCLUSIONS
A FMA method is established to monitor the immune response in HFRS patients, which has better sensitivity than ELISA, and thus provides a useful tool for study of HFRS.
Zhang
Jie
J
Central Laboratory, Shaanxi Provincial People's Hospital, Xi'an, China. zhangjie810@yahoo.com.cn
Lin
Yuan
Y
Jin
Bo-quan
BQ
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D018063:Hantaan virus / Q000276:immunology; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood* / Q000175:diagnosis / Q000821:virology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha