title: Polyphenol-stabilized tubular elastin scaffolds for tissue engineered vascular grafts.
abstract: Tissue-engineered vascular grafts require elastic, acellular porous scaffolds with controlled biodegradability and properties matching those of natural arteries. Elastin would be a desirable component for such applications, but elastin does not easily regenerate experimentally. Our approach is to develop tubular elastin scaffolds using decellularization and removal of collagen from porcine carotid arteries ( approximately 5 mm diameter) using alkaline extraction. Because elastin is susceptible to rapid degeneration after implantation, scaffolds were further treated with penta-galloyl glucose (PGG), an established polyphenolic elastin-stabilizing agent. Scaffolds were compared in vitro with detergent-decellularized arteries for structure, composition, resistance to degradation, mechanical properties, and cytotoxicity and in vivo for cell infiltration and remodeling potential. Results showed effective decellularization and almost complete collagen removal by alkaline extraction. PGG-treated elastin scaffolds proved to be resistant to elastase digestion in vitro, maintained their cylindrical shapes, showed high resistance to burst pressures, and supported growth of endothelial cells and fibroblasts. In vivo results showed that PGG treatment reduced the rate of elastin biodegradation and controlled cell infiltration but did not hamper new collagen and proteoglycan deposition and secretion of matrix-degrading proteases. Alkali-purified, PGG-treated tubular arterial elastin scaffolds exhibit many desirable properties to be recommended for clinical applications as vascular grafts.
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, South Carolina 29634, USA.
authors.forename: Ting-Hsien
authors.initials: TH
authors.lastname: Chuang
authors.forename: Christopher
authors.initials: C
authors.lastname: Stabler
authors.forename: Agneta
authors.initials: A
authors.lastname: Simionescu
authors.forename: Dan T
authors.initials: DT
authors.lastname: Simionescu
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D004549:Elastin / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D047348:Hydrolyzable Tannins / Q000737:chemistry*; D007150:Immunohistochemistry; D008297:Male; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D059808:Polyphenols; C435084:pentagalloylglucose; D004549:Elastin