title: Method validation of microcystins in water and tissue by enhanced liquid chromatography tandem mass spectrometry.
abstract: A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method has been developed and validated to identify and quantify trace levels of cyanotoxins or microcystins (MC) in water, bivalves and fish tissue with enhanced sensitivity and specificity. The method enables confirmation and quantification of six MCs (MC-LA, LF, LR, LW, RR and YR) with a single chromatographic run. The applied chromatography also allows determination of certain MC metabolites (Desmethyl-LR and -RR). By using LC-ESI-MS/MS in multiple reaction monitoring (MRM) mode, the limit of detection and quantitation for the microcystins studied, were determined to be between 0.2 and 1 pg on column (5:1 S/N ratio). These values are below the 2 pg detection limits found in the available literature.
authors.affiliation: California Department of Fish and Game and Moss Landing Marine Laboratory, Rancho Cordova, CA 95670, USA. amekebri@ospr.dfg.ca.gov
authors.forename: A
authors.initials: A
authors.lastname: Mekebri
authors.forename: G J
authors.initials: GJ
authors.lastname: Blondina
authors.forename: D B
authors.initials: DB
authors.lastname: Crane
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005068:Eutrophication; D005399:Fishes; D005618:Fresh Water / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D052998:Microcystins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D052998:Microcystins