Synthesis and characterization of a chondroitin sulfate-polyethylene glycol corneal adhesive.
OBJECTIVE
To describe the synthesis of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and characterize its physical and biological properties in vitro and in vivo.


METHODS
Johns Hopkins University and a research facility, Baltimore, Maryland, USA.


METHODS
Metabolic activity (WST-1 reagent) was used to evaluate the cytocompatibility of the adhesive with rabbit primary epithelial, stromal, and endothelial cells. Full-thickness corneal incisions (3.0 mm) in ex vivo porcine eyes were sealed with the adhesive, and burst pressure was evaluated to determine the effectiveness of the material in maintaining intraocular pressure (IOP). Finally, a partial-thickness incision was made in a swine cornea and then sealed using the adhesive. Two weeks postoperatively, both eyes were enucleated and examined grossly and histologically.


RESULTS
In vitro results showed cytocompatibility of the tissue adhesive with corneal cells and an ability to seal full-thickness corneal incisions exposed to IOPs of 200 mm Hg and higher. Histological evidence from in vivo data confirmed that the CS-PEG material is biodegradable, induces minimal inflammatory response, resists epithelial cell ingrowth, and does not induce scar formation.


CONCLUSIONS
The new adhesive was effective in restoring IOP and withstanding pressures greater than 200 mm Hg after being applied to a full-thickness corneal incision. The adhesive material was biocompatible with the 3 types of cells found in corneal tissue. When the adhesive was implanted in a live swine model, no adverse side effects were observed.
Strehin
Iossif
I
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Ambrose
Winnette McIntosh
WM
Schein
Oliver
O
Salahuddin
Afrah
A
Elisseeff
Jennifer
J
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry*; D003315:Cornea / Q000473:pathology / Q000601:surgery*; D003319:Corneal Stroma / Q000187:drug effects; D004728:Endothelium, Corneal / Q000187:drug effects; D019573:Epithelium, Corneal / Q000187:drug effects; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology; D008297:Male; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry*; D011817:Rabbits; D013529:Surgical Wound Dehiscence / Q000473:pathology / Q000517:prevention & control*; D013552:Swine; D014014:Tissue Adhesives / Q000138:chemical synthesis* / Q000494:pharmacology* / Q000633:toxicity; D014945:Wound Healing / Q000187:drug effects*
D001672:Biocompatible Materials; D014014:Tissue Adhesives; D011092:Polyethylene Glycols; D002809:Chondroitin Sulfates