Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana.
Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H(2)O(2) production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H(2)O(2) and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H(2)O(2) derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.
Hu
Xiangyang
X
Kunming Institute of Botany, Institute of Tibet Plateau Research at Kunming, Chinese Academy of Science, Kunming, 650204, China. huxiangyang@mail.kib.ac.cn
Neill
Stevn J
SJ
Yang
Yongping
Y
Cai
Weiming
W
D000331:Aequorin / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D010838:Phytophthora / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013229:Stearic Acids / Q000378:metabolism*; D013997:Time Factors
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005656:Fungal Proteins; D054883:Oxylipins; D010455:Peptides; D013229:Stearic Acids; D000331:Aequorin; C011006:jasmonic acid; D006861:Hydrogen Peroxide; C416525:OPR3 protein, Arabidopsis; D010088:Oxidoreductases; D008084:Lipoxygenase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase; D002118:Calcium