Effect of different extraction protocols on anticancer and antioxidant activities of Berberis koreana bark extracts.
High-pressure extraction and ultrasonification extraction techniques were employed to extract bioactive compounds from Berberis koreana. This study aimed to determine the effect of ultrasonification in a high pressure process on the extraction yield, and the anticancer and antioxidant activities of the B. koreana bark extract. The effect of high-pressure extraction time when carried out for 5 and 15 min (HP5 and HP15) was also investigated. The best extraction yield with maximum percentage of phenolic compounds was obtained using high pressure with sonification (HPWS) extraction method. Experimental results indicated that HPWS altered the antioxidant activities, including the scavenging capacity of diphenylpicrylhydrazyl (DPPH) and xanthine oxidase. HP5 and HP15 with conventional extraction have almost similar bioactivity, but showed lower antioxidant and anticancer activities compared to HPWS. The results showed that the application of ultrasonification improved the extraction efficiency for bioactive compounds and, as deduced from chromatographic profiles, it may have allowed the release of new compounds. The scanning electron microscope (SEM) showed evidence of rupturing of the tissue surface treated with HPWS, in contrast to conventional extraction, HP5, and HP15. The HPWS extraction was not only more efficient but also convenient for the recovery and purification of the active compounds of hard plant tissues.
Qadir
Syed Abdul
SA
Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University, Chunchon 200-701, Republic of Korea.
Kwon
Min Chul
MC
Han
Jae Gun
JG
Ha
Ji He
JH
Chung
Hyang Suk
HS
Ahn
Juhee
J
Lee
Hyeon Yong
HY
D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D028142:Berberis / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology
D000970:Antineoplastic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts