title: Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.
abstract: Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.
authors.affiliation: Department of Biological Sciences, Florida International University, Miami, Florida 33199, USA. S.Raju@Sheffield.ac.uk
authors.forename: Raju
authors.initials: R
authors.lastname: Sekar
authors.forename: Longin T
authors.initials: LT
authors.lastname: Kaczmarsky
authors.forename: Laurie L
authors.initials: LL
authors.lastname: Richardson
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D005615:Freezing; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S