[Influence of tea polyphenols on the damage of fibrinolytic system induced by homocysteine in human umbilical vein endothelial cells].
OBJECTIVE
To study the influence of tea polyphenols (TP) on cells form and fibrinolytic system which focused on tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) induced by homocysteine (Hcy) in human umbilical vein endothelial cells (HUVEC).


METHODS
HUVEC were divided into two groups, one was administered with TP and Hey at the same time, the other was firstly treated with Hey for 12 hours and followed by administering TP. Each group was divided into some groups depending on different concentrations of TP. The t-PA and PAI-1 levels of HUVEC supernatant were determined by ELISA assay.


RESULTS
Compared with the groups that just treated with Hcy, medium concentration (4 and 8 microg/ml) and high concentration (16 microg/ml) of TP could strengthen cells stereoscopic effect and decrease endochylema particles; the results of ELISA demonstrated that the concentrations of PAI-1 were increased in the groups that just treated with Hey compared with control group (P < 0.05), administrations of medium and high dose of TP could decrease the concentration of PAI-1, and the concentration of PAI-1 were decreased depending on the increase of TP concentration (P < 0.05), there was no difference between low concentration TP group and the group only treated with Hcy. There were no differences on the concentrations of t-PA among each groups.


CONCLUSIONS
The proper concentration of TP could restore the damage of HUVEC form and decrease the concentration of PAI-1 induced by Hey.
Pei
Jingjing
J
School of Public Health, Shandong University, Ji'nan 250012, China. pjj2001@163.com
Guo
Shuai
S
Zhang
Chunling
C
Xie
Keqin
K
D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D005342:Fibrinolysis / Q000187:drug effects / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology*; D006710:Homocysteine / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000473:pathology / Q000503:physiopathology*; D010636:Phenols / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*; D010959:Tissue Plasminogen Activator / Q000032:analysis*; D014471:Umbilical Veins / Q000166:cytology
D005419:Flavonoids; D010636:Phenols; D017395:Plasminogen Activator Inhibitor 1; D059808:Polyphenols; D013662:Tea; D006710:Homocysteine; D010959:Tissue Plasminogen Activator