Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae.
A Saccharomyces cerevisiae strain deficient in phosphatidylethanolamine methyltransferase that exhibits choline auxotrophy grew in the presence of dioctanoyl phosphatidylcholine (diC8PC). We isolated and characterized mutants defective in growth in the diC8PC-containing medium. Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains.
Ono
Yusuke
Y
Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
Fukuda
Ryouichi
R
Ohta
Akinori
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010713:Phosphatidylcholines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C478771:Lem3 protein, S cerevisiae; D026901:Membrane Transport Proteins; D010713:Phosphatidylcholines; D029701:Saccharomyces cerevisiae Proteins