Electrophoretic separation of proteins in capillaries filled with poly(ethylene oxide)-stabilized silver nanoparticles.
Poly(ethylene oxide)-stabilized silver nanoparticles (PEO-SSNPs) were synthesized and investigated for their potential use in capillary electrophoretic separation of proteins. It was found that the PEO-SSNPs were relatively stable against changes of the buffer ionic strength, while they began to aggregate with increase of buffer pH. Adding 0.024 g/L PEO-SSNPs to 30 mmol/L phosphate buffer (pH 2.66) led to obvious increase in the peak heights of albumin bovine and albumin egg. Also, the separation efficiency for all the standards enhanced due to the suppressed wall-adsorption of proteins in the presence of the nanoparticles. The above experiments suggest that the PEO-SSNPs are effective pseudostationary phase for CE separation of proteins.
Qin
Weidong
W
College of Chemistry, Beijing Normal University, Beijing 100875, PR China. qinwd@bnu.edu.cn
Tursen
Janar
J
D000327:Adsorption; D000418:Albumins / Q000302:isolation & purification*; D003102:Colloids / Q000138:chemical synthesis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006454:Hemoglobins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D013997:Time Factors
D000418:Albumins; D003102:Colloids; D006454:Hemoglobins; D012834:Silver; D011092:Polyethylene Glycols; D009113:Muramidase