[Efficient detection and analysis of linear plasmid in Stremptomyces Men-myco-93-63].
OBJECTIVE
To develop an efficient method to detect and analyze the endogenous plasmid in Stremptomyces Men-myco-93-63.


METHODS
According to the property of 'the linear chromosomes and plasmids of Stremptomyces spp.have proteins covalently bound to the 5'ends of the DNA', a novel method was established to detect the type, configuration and size of the Streptomyces spp. plasmids by improving the preparation of agarose embedded block. The method can also show whether the linear plasmids have proteins covalently bound to the DNA from both the positive and negative aspects.


RESULTS
The type and configuration of two plasmids were determined efficiently from Stremptomyces roseoflavus Men-myco-93-63 and verified by the two-dimensional electrophoresis. The speculated sizes of these plasmids were 47 kb and 53 kb, respectively.


CONCLUSIONS
Two linear plasmids were detected successfully from Stremptomyces roseoflavus Men-myco-93-63 by a new method.
Sun
Weiming
W
College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China. swimming122@yahoo.com.cn
Guo
Wei
W
Daqun
Liu
L
D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008297:Male; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology
D004269:DNA, Bacterial