title: Increased mRNA expression of ADAMTS metalloproteinases in metastatic foci of head and neck cancer.
abstract: BACKGROUND
  Although contribution of matrix metalloproteinases in cancer progression and dissemination is now well known, roles of recently discovered metalloproteinases, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), in cancer development and progression remain mostly unknown.
  
  
  METHODS
  Here we examined the mRNA expression pattern of 6 members of ADAMTS aggrecanases (1, 4, 5, 8, 9, and 15) in primary head and neck cancer with and without metastasis by real-time reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  Expression levels of ADAMTS mRNAs were lower in the majority of the primary tumors as compared with the controls. On the other hand, the expression levels of all of the ADAMTS mRNAs except ADAMTS4 were higher in the metastatic foci than in their corresponding primary tumors, which suggest that characteristics of the cancer cell population are different in the primary tumor and metastatic focus.
  
  
  CONCLUSIONS
  Our findings suggest a metastasis model proposing accumulation of a subtype of cancer cells with high metastatic capacity within heterogenous primary tumor cell population.
authors.affiliation: Department of Molecular Biology and Biochemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Shikatacho, Okayama, Japan.
authors.forename: Kadir
authors.initials: K
authors.lastname: Demircan
authors.forename: Esra
authors.initials: E
authors.lastname: Gunduz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gunduz
authors.forename: Levent Bekir
authors.initials: LB
authors.lastname: Beder
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirohata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Mehmet Zeynel
authors.initials: MZ
authors.lastname: Cilek
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamanaka
authors.forename: Kenji
authors.initials: K
authors.lastname: Shimizu
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Ninomiya
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000071116:ADAMTS5 Protein; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000556:secondary*; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D051722:ADAM Proteins; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human