[The influence of circadian clock gene on mouse sperm fertility in RNAi].
OBJECTIVE
To study the influence of circadian Clock gene on the fertilization ability of sperm via attenuating the expression of Clock with RNAi in male mice.


METHODS
After the injection of RNAi plasmid into mice testes, the expression of circadian Clock gene of testis tissue was determined by western Blotting. The fertilization ability of sperm was evaluated by various indices, including litter size in mated female mice, sperm count, sperm motility, in vitro fertilization (IVF) rate and acrosome development.


RESULTS
The RNAi plasmid targeting circadian gene Clock attenuated the expression of CLOCK in mice testis, reduced in vitro fertilization CIVF) rate and the conception rate in viva, and also decreased sperm acrosin activity.


CONCLUSIONS
Circadian gene Clock is related to the reproductive function in male mice. It probably affect sperm fertilization ability by regulating sperm acrosin activity.
Jiang
Xiao-hui
XH
Department of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhang
Lu
L
Wang
Yu-hui
YH
Liu
Yan-you
YY
Jiang
Zhou
Z
Wang
Zheng-rong
ZR
D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics* / Q000378:metabolism; D005298:Fertility / Q000235:genetics*; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013075:Sperm Capacitation / Q000235:genetics*
D034741:RNA, Small Interfering; D056926:CLOCK Proteins; C538944:Clock protein, mouse