Improved infiltration of stem cells on electrospun nanofibers.
Nanofibrous scaffolds have been recently used in the field of tissue engineering because of their nano-size structure which promotes cell attachment, function, proliferation and infiltration. In this study, nanofibrous polyethersulfone (PES) scaffolds was prepared via electrospinning. The scaffolds were surface modified by plasma treatment and collagen grafting. The surface changes then investigated by contact angle measurements and FTIR-ATR. The results proved grafting of the collagen on nanofibers surface and increased hydrophilicity after plasma treatment and collagen grafting. The cell interaction study was done using stem cells because of their ability to differentiate to different kinds of cell lines. The cells had normal morphology on nanofibers and showed very high infiltration through collagen grafted PES nanofibers. This infiltration capability is very useful and needed to make 3D scaffolds in tissue engineering.
Shabani
Iman
I
Polymer Engineering Department, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
Haddadi-Asl
Vahid
V
Seyedjafari
Ehsan
E
Babaeijandaghi
Farshad
F
Soleimani
Masoud
M
D018914:Cells, Immobilized; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013234:Stem Cells / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D013450:Sulfones / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone