title: Production of a reporter transgenic pig for monitoring Cre recombinase activity.
abstract: The pig is thought to be the most suitable non-human source of organs for xenotransplantation and is widely used as a model of human disease. Using pigs as disease models requires the design of conditional Cre recombinase-loxP gene modifications, which, in turn, requires a Cre-expressing pig with defined patterns of expression controlled by the use of a tissue-specific promoter. In order to monitor Cre recombinant expression in vivo, it is important to create a reporter strain. We have generated reporter a pig that is based on a single vector that drives the ubiquitous expression of the enhanced green fluorescent protein (EGFP). The EGFP gene is expressed only after Cre-mediated excision of loxP-flanked stop sequences. These reporter transgenic pigs will be of great value for monitoring Cre recombinase activity in vivo.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China. lilitonghappy@hotmail.com
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
authors.forename: Tiedong
authors.initials: T
authors.lastname: Wang
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
authors.forename: Limei
authors.initials: L
authors.lastname: Chen
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Song
authors.forename: Daibang
authors.initials: D
authors.lastname: Nie
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Chen
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004195:Disease Models, Animal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases