MicroRNA expression profiling during neural differentiation of mouse embryonic carcinoma P19 cells.
MicroRNAs (or miRNAs) are small non-coding RNAs (21-25 nucleotides) that are involved in a wide range of activities related to the development and differentiation of cells. Comparison of the miRNA expression profiles of mouse P19 embryonic carcinoma cells with those of differentiated neural stem cells showed that the expression level of 65 miRNAs changed (2-fold) after differentiation. MiR-124a was dramatically upregulated (more than 20-fold) while miRNAs of the miR-302 family and those in the miR-290-295 cluster were strongly down-regulated. Further analysis revealed that some important factors such as Oct4 and Sox2 appeared to be involved in the regulation of these miRNAs. These results may contribute to a better understanding of miRNA-regulated neural differentiation in early mouse embryos.
Huang
Bing
B
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, Peopleos Republic of China.
Li
Wei
W
Zhao
Botao
B
Xia
Caihong
C
Liang
Ruqiang
R
Ruan
Kangcheng
K
Jing
Naihe
N
Jin
Youxin
Y
D000818:Animals; D018236:Carcinoma, Embryonal / Q000235:genetics / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics* / Q000502:physiology; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D018243:Teratocarcinoma / Q000235:genetics / Q000473:pathology
D035683:MicroRNAs