Construction of gene-targeting vectors by recombineering.
Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by gamma phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.
Lee
Song-Choon
SC
Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Wang
Wei
W
Liu
Pentao
P
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D007763:Lac Operon; D051379:Mice; D018345:Mice, Knockout; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic