title: Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver.
abstract: To investigate the role of DNA methylation during human development, we developed Methyl-seq, a method that assays DNA methylation at more than 90,000 regions throughout the genome. Performing Methyl-seq on human embryonic stem cells (hESCs), their derivatives, and human tissues allowed us to identify several trends during hESC and in vivo liver differentiation. First, differentiation results in DNA methylation changes at a minimal number of assayed regions, both in vitro and in vivo (2%-11%). Second, in vitro hESC differentiation is characterized by both de novo methylation and demethylation, whereas in vivo fetal liver development is characterized predominantly by demethylation. Third, hESC differentiation is uniquely characterized by methylation changes specifically at H3K27me3-occupied regions, bivalent domains, and low density CpG promoters (LCPs), suggesting that these regions are more likely to be involved in transcriptional regulation during hESC differentiation. Although both H3K27me3-occupied domains and LCPs are also regions of high variability in DNA methylation state during human liver development, these regions become highly unmethylated, which is a distinct trend from that observed in hESCs. Taken together, our results indicate that hESC differentiation has a unique DNA methylation signature that may not be indicative of in vivo differentiation.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA.
authors.forename: Alayne L
authors.initials: AL
authors.lastname: Brunner
authors.forename: David S
authors.initials: DS
authors.lastname: Johnson
authors.forename: Si Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Anton
authors.initials: A
authors.lastname: Valouev
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Reddy
authors.forename: Norma F
authors.initials: NF
authors.lastname: Neff
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Anton
authors.forename: Catherine
authors.initials: C
authors.lastname: Medina
authors.forename: Loan
authors.initials: L
authors.lastname: Nguyen
authors.forename: Eric
authors.initials: E
authors.lastname: Chiao
authors.forename: Chuba B
authors.initials: CB
authors.lastname: Oyolu
authors.forename: Gary P
authors.initials: GP
authors.lastname: Schroth
authors.forename: Devin M
authors.initials: DM
authors.lastname: Absher
authors.forename: Julie C
authors.initials: JC
authors.lastname: Baker
authors.forename: Richard M
authors.initials: RM
authors.lastname: Myers
mesh_terms: D001665:Binding Sites; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D002874:Chromosome Mapping; D016000:Cluster Analysis; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D015894:Genome, Human / Q000235:genetics; D006657:Histones / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D006657:Histones; D008239:Lysine