Amino acid alterations in Gag that confer the ability to grow in simian cells on HIV-1 are located at a narrow CA region.
We previously generated a prototype monkey-tropic human immunodeficiency virus type 1 (HIV-1) designated NL-DT5R. This viral clone has a small region of simian immunodeficiency virus (SIV) within Gag capsid (CA) protein and also SIV Vif protein, but displays a poor growth phenotype in simian cells. To improve the growth potential of NL-DT5R, we have constructed a series of its gag variant viruses. Out of fourteen viral clones generated, five were infectious for simian HSC-F cells, and two of the infectious variants grew similarly with NL-DT5R. Taking their genome structures into consideration, our data here clearly show that a narrow CA region within the Gag protein, i.e., the domain around cyclophilin A (CypA)-binding loop, is critical for the growth ability of HIV-1 in simian cells.
Nagao
Tamiko
T
Department of Virology, Institute of Health Biosciences, the University of Tokushima Graduate School, Japan.
Hatcho
Kazuki
K
Doi
Naoya
N
Fujiwara
Sachi
S
Adachi
Akio
A
Nomaguchi
Masako
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D021984:Cyclophilin A / Q000032:analysis* / Q000502:physiology; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000821:virology; D015683:Gene Products, gag / Q000032:analysis* / Q000502:physiology*; D016346:Gene Products, vif / Q000032:analysis / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D009154:Mutation; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D013601:T-Lymphocytes / Q000821:virology*
D000596:Amino Acids; D015683:Gene Products, gag; D016346:Gene Products, vif; D021984:Cyclophilin A