[Effects of FGF5 gene on fibre traits on Inner Mongolian cashmere goats].
Two pairs primers were designed base on the known sequence for amplification of FGF5 gene in the Inner Mongolian cashmere goats herd. It was found that a restrict fragment length polymorphism within the exon 1 of FGF5 gene from the second pair of primers using PCR-SSCP and PCR-RFLP. PCR products after recovered and purified, were sequenced that randomly selected from different genotypes. It was revealed a synonymous single base mutation (C-->T) for the AB genotype. The allele A was found to be predominant in Inner Mongolian cashmere goats and the allele and genotype frequencies in our sample coincident with the Hardy-Weinberg equilibrium. When analyzed for the association with fibre traits, this FGF5 SNP was found to have significant effect on cashmere fibre stretched length (P<0.01) and cashmere rate(P<0.05), but have slightly effect on others fibre traits (P>0.05). Cashmere fibre stretched length (P<0.01) and cashmere rate (P<0.05) in individuals of genotype AB was significantly higher than that in individuals of genotype AA.
Liu
Hai-Ying
HY
College of Animal Science and Veterinary Science, Shenyang Agricultural University, Shenyang 100161, China. liuhaiyingw@yahoo.com.cn
Yang
Gui-Qin
GQ
Zhang
Wei
W
Zhu
Xiao-Ping
XP
Jia
Zhi-Hai
ZH
D000818:Animals; D002681:China; D051521:Fibroblast Growth Factor 5 / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational
D051521:Fibroblast Growth Factor 5