title: Dicing of viral replication intermediates during silencing of latent Drosophila viruses.
abstract: Previous studies revealed roles for RNA interference (RNAi) in the immediate cellular response to viral infection in plants, nematodes and flies. However, little is known about how RNAi combats viruses during persistent or latent infections. Our analysis of small RNAs cloned from Drosophila cells latently infected with Flock House Virus (FHV) failed to reveal signatures of bulk degradation of the viral genome. Instead, this + strand virus specifically generated Dicer-2-dependent, 21-nucleotide siRNAs that derived in equal proportion from + and - strands. Curiously, luciferase reporters that are fully complementary to abundant viral siRNAs were poorly repressed. Moreover, although the viral siRNAs that were incorporated into an effector complex associated with Argonaute2, bulk FHV siRNAs in latently infected cells were not loaded into any Argonaute protein. Together, these data suggest that direct dicing of viral replication intermediates plays an important role in maintaining the latent viral state. In addition, the denial of bulk viral siRNAs from effector complexes suggests that criteria beyond the structural competency of RNA duplexes influence the assembly of functional silencing complexes.
authors.affiliation: Department of Developmental Biology, Sloan-Kettering Institute, 1017 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, NY 10065, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Flynt
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Raquel
authors.initials: R
authors.lastname: Martin
authors.forename: Eric C
authors.initials: EC
authors.lastname: Lai
mesh_terms: D000818:Animals; D060565:Argonaute Proteins; D002460:Cell Line; D004330:Drosophila / Q000821:virology*; D029721:Drosophila Proteins / Q000378:metabolism / Q000502:physiology*; D020365:RNA Helicases / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex / Q000378:metabolism; D043244:Ribonuclease III; D017735:Virus Latency; D014779:Virus Replication
chemical_list: C433807:AGO2 protein, Drosophila; D060565:Argonaute Proteins; D029721:Drosophila Proteins; D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex; C478069:DCR-2 protein, Drosophila; D043244:Ribonuclease III; D020365:RNA Helicases