HPV genotyping from invasive cervical cancer in Chile.
OBJECTIVE
To determine the prevalence rates of the different HPV types in cervical cancer lesions in Chile to facilitate the development of prophylactic human papillomavirus (HPV) vaccines effective for that country.


METHODS
Biopsy samples of 312 cervical cancer lesions were assessed for HPV type by reverse-line blotting assay.


RESULTS
HPV DNA was found in 94.2% of the lesions, 67.2% harboring 1 viral type and the remainder harboring more than 1 type. HPV-16 was the most frequent type in single infections (50.5%), followed by HPV-18 (7.8%), HPV-31 (2.4%), and HPV-45 (2.0%). HPV-16 was also present in 98.7% of dual and multiple infections, its most frequent association being with HPV-18.


CONCLUSIONS
HPV types 16, 18, 31, and 45, alone or combined with other types, were observed in the biopsy samples of up to 80.5% of cervical cancer lesions.
Roa
Juan Carlos
JC
Molecular Pathology Laboratory, Department of Pathology, Universidad de la Frontera School of Medicine, Temuco, Chile.
Garcia
Patricia
P
Gomez
Jorge
J
Fernández
Wanda
W
Gaete
Fancy
F
Espinoza
Arturo
A
Lepetic
Alejandro
A
Suarez
Eugenio
E
D000230:Adenocarcinoma / Q000453:epidemiology / Q000821:virology*; D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology*; D002677:Chile / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult
D004279:DNA, Viral