[Effect of high dose apigenin on antioxidase activity and DNA damage in rats].
OBJECTIVE
To investigate the effect of high doses apigenin on antioxidase activity of and DNA oxidative damages in rats.


METHODS
100 Wistar rats were randomly divided into five groups including control, dose one to four groups. The trial lasted 13 weeks, the blood samples and liver of rats were collected at the end of the experiment. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and levels of malondialdehyde (MDA) were determinde, 8-OHdG content, which is indicative of DNA damage were assessed.


RESULTS
At 2 g/kg and 4 g/kg apigenin, the activity of SOD and GSH-Px in male rat liver significantly decreased, at 2 g/kg bw dose, the activity of male rat liver GST also greatly decreased. At the dose of 4 g/kg apigenin, GSH-Px activity in serum of both sex rats significantly decreased, its male rat GST activity at dose of 8 g/kg apigenin also greatly decreased.


CONCLUSIONS
High dose apigenin could decrease antioxidase activity of male rat, but, it didn't cause DNA damage.
Sui
Haixia
H
Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China. haixiasui@yahoo.com.cn
Xu
Haibin
H
Yin
Shi-an
SA
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D047310:Apigenin / Q000008:administration & dosage / Q000494:pharmacology*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*; D014886:Weaning
D047310:Apigenin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D003849:Deoxyguanosine