title: Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.
abstract: Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Hurst
authors.forename: S E
authors.initials: SE
authors.lastname: Kidd
authors.forename: C O
authors.initials: CO
authors.lastname: Morrissey
authors.forename: E
authors.initials: E
authors.lastname: Snelders
authors.forename: W J G
authors.initials: WJ
authors.lastname: Melchers
authors.forename: M V
authors.initials: MV
authors.lastname: Castelli
authors.forename: E
authors.initials: E
authors.lastname: Mellado
authors.forename: K
authors.initials: K
authors.lastname: Simmon
authors.forename: C A
authors.initials: CA
authors.lastname: Petti
authors.forename: S
authors.initials: S
authors.lastname: Richardson
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: A M
authors.initials: AM
authors.lastname: Romanelli
authors.forename: B L
authors.initials: BL
authors.lastname: Wickes
authors.forename: H A
authors.initials: HA
authors.lastname: de Valk
authors.forename: C H W
authors.initials: CH
authors.lastname: Klaassen
authors.forename: S Arunmozhi
authors.initials: SA
authors.lastname: Balajee
mesh_terms: D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D005838:Genotype; D016533:Mycological Typing Techniques / Q000379:methods* / Q000592:standards*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000592:standards*
chemical_list: D004271:DNA, Fungal