title: Comparison of routine bench and molecular diagnostic methods in identification of Burkholderia pseudomallei.
abstract: This study compared the identification of Burkholderia pseudomallei with that of related organisms. Bench tests and latex agglutination were compared with molecular identification. Using bench tests and latex agglutination alone, 100% (30/30) of B. pseudomallei isolates were correctly identified. Amoxicillin-clavulanate susceptibility testing was also a good and simple discriminatory test.
authors.affiliation: School of Veterinary & Biomedical Sciences, James Cook University, Townsville, Queensland, Australia.
authors.forename: Kelly
authors.initials: K
authors.lastname: Hodgson
authors.forename: Cathy
authors.initials: C
authors.lastname: Engler
authors.forename: Brenda
authors.initials: B
authors.lastname: Govan
authors.forename: Natkunam
authors.initials: N
authors.lastname: Ketheesan
authors.forename: Robert
authors.initials: R
authors.lastname: Norton
mesh_terms: D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D008554:Melioidosis / Q000175:diagnosis*; D008826:Microbial Sensitivity Tests; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010088:Oxidoreductases / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D019980:Amoxicillin-Potassium Clavulanate Combination; D010088:Oxidoreductases