title: Quantification of dengue virus type 3 RNA in fatal and non-fatal cases in Brazil, 2002.
abstract: We examined levels of dengue virus type 3 (DENV-3) RNA in association with the type of infection (primary or secondary) in 42 patients with fatal and non-fatal outcomes in Rio de Janeiro, Brazil, 2002. Subjects with fatal outcomes had mean virus titers significantly higher than those who survived (12.5 vs. 7.9 log(10) RNA copies/ml). Because primary infections were confirmed among the fatal cases (52.1%), antibody-dependent enhancement alone did not explain all the cases of severe disease in this study population. These findings suggest that high levels of DENV-3 may have contributed to the severe form of dengue in Rio de Janeiro, 2002.
authors.affiliation: Laboratory of Flavivirus, Oswaldo Cruz Institute, 21045-900 Rio de Janeiro, Brazil. joselio@ioc.fiocruz.br
authors.forename: Josélio M G
authors.initials: JM
authors.lastname: de Araújo
authors.forename: Ana M B
authors.initials: AM
authors.lastname: de Filippis
authors.forename: Hermann G
authors.initials: HG
authors.lastname: Schatzmayr
authors.forename: Eliane S M
authors.initials: ES
authors.lastname: de Araújo
authors.forename: Constança
authors.initials: C
authors.lastname: Britto
authors.forename: Maria A
authors.initials: MA
authors.lastname: Cardoso
authors.forename: Luiz A B
authors.initials: LA
authors.lastname: Camacho
authors.forename: Rita M R
authors.initials: RM
authors.lastname: Nogueira
mesh_terms: D019067:Antibody-Dependent Enhancement / Q000276:immunology; D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000401:mortality / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007074:Immunoglobulin G; D012367:RNA, Viral