Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses.
nsp1 protein of severe acute respiratory syndrome coronavirus (SARS-CoV), a group 2b CoV, suppresses host gene expression by promoting host mRNA degradation and translation inhibition. The present study analyzed the activities of nsp1 proteins from the group 2 bat CoV strains Rm1, 133, and HKU9-1, belonging to groups 2b, 2c, and 2d, respectively. The host mRNA degradation and translational suppression activities of nsp1 of SARS-CoV and Rm1 nsp1 were similar and stronger than the activities of the nsp1 proteins of 133 and HKU9-1. Rm1 nsp1 expression in trans strongly inhibited the induction of type I interferon (IFN-I) and IFN-stimulated genes in cells infected with an IFN-inducing SARS-CoV mutant, while 133 and HKU9-1 nsp1 proteins had relatively moderate IFN-inhibitory activities. The results of our studies suggested a conserved function among nsp1 proteins of SARS-CoV and group 2 bat CoVs.
Tohya
Yukinobu
Y
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.
Narayanan
Krishna
K
Kamitani
Wataru
W
Huang
Cheng
C
Lokugamage
Kumari
K
Makino
Shinji
S
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002685:Chiroptera / Q000821:virology; D017934:Coronavirus / Q000378:metabolism / Q000502:physiology*; D018352:Coronavirus Infections / Q000235:genetics* / Q000821:virology; D005786:Gene Expression Regulation; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000378:metabolism / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D007370:Interferon Type I; D017361:Viral Nonstructural Proteins; C521592:Nsp1 protein, SARS coronavirus; D012324:RNA-Dependent RNA Polymerase