title: Glycine betaine may have opposite effects on protein stability at high and low pH values.
abstract: The compatible osmolyte glycine betaine (GB) is the most efficient osmoprotectant and best excluder from the protein surface. It can reverse protein aggregation and correct mutant protein defects and counter the harmful effects of urea and salts in vivo and in vitro. In this study we have investigated the pH dependence of the stabilizing effect of GB on three different proteins, namely, alpha-lactalbumin (alpha-LA), lysozyme and ribonuclease-A (RNase-A). We show here that (a) GB stabilizes RNase-A at all pH values, and (b) GB has opposite effects on two proteins at high pH and low pH values, namely, alpha-LA and lysozyme. This conclusion was reached by determining T(m) (midpoint of denaturation), DeltaH(m) (denaturational enthalpy change at T(m)), DeltaC(p) (constant-pressure heat capacity change) and DeltaG(D)(o) (denaturational Gibbs energy change at 25 degrees C) of proteins in the presence of different GB concentrations. Another conclusion of this study is that DeltaH(m) and DeltaC(p) are not significantly changed in the presence of GB. This study suggests that other methylated glycine osmolytes may also behave in the same manner.
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Laishram R
authors.initials: LR
authors.lastname: Singh
authors.forename: Tanveer Ali
authors.initials: TA
authors.lastname: Dar
authors.forename: Safikur
authors.initials: S
authors.lastname: Rahman
authors.forename: Shazia
authors.initials: S
authors.lastname: Jamal
authors.forename: Faizan
authors.initials: F
authors.lastname: Ahmad
mesh_terms: D001622:Betaine / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D001622:Betaine