title: The role of oxygen in yeast metabolism during high cell density brewery fermentations.
abstract: The volumetric productivity of the beer fermentation process can be increased by using a higher pitching rate (i.e., higher inoculum size). However, the decreased yeast net growth observed in these high cell density fermentations can have a negative impact on the physiological stability throughout subsequent yeast generations. The use of different oxygen conditions (wort aeration, wort oxygenation, yeast preoxygenation) was investigated to improve the growth yield during high cell density fermentations and yeast metabolic and physiological parameters were assessed systematically. Together with a higher extent of growth (dependent on the applied oxygen conditions), the fermentation power and the formation of unsaturated fatty acids were also affected. Wort oxygenation had a significant decreasing effect on the formation of esters, which was caused by a decreased expression of the alcohol acetyl transferase gene ATF1, compared with the other conditions. Lower glycogen and trehalose levels at the end of fermentation were observed in case of the high cell density fermentations with oxygenated wort and the reference fermentation. The expression levels of BAP2 (encoding the branched chain amino acid permease), ERG1 (encoding squalene epoxidase), and the stress responsive gene HSP12 were predominantly influenced by the high cell concentrations, while OLE1 (encoding the fatty acid desaturase) and the oxidative stress responsive genes SOD1 and CTT1 were mainly affected by the oxygen availability per cell. These results demonstrate that optimisation of high cell density fermentations could be achieved by improving the oxygen conditions, without drastically affecting the physiological condition of the yeast and beer quality.
authors.affiliation: Centre for Malting and Brewing Science, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, Box 2463, 3001 Heverlee, Belgium. Pieter.Verbelen@biw.kuleuven.be
authors.forename: P J
authors.initials: PJ
authors.lastname: Verbelen
authors.forename: S M G
authors.initials: SM
authors.lastname: Saerens
authors.forename: S E
authors.initials: SE
authors.lastname: Van Mulders
authors.forename: F
authors.initials: F
authors.lastname: Delvaux
authors.forename: F R
authors.initials: FR
authors.lastname: Delvaux
mesh_terms: D001515:Beer / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D004952:Esters / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005285:Fermentation; D006003:Glycogen / Q000032:analysis; D010100:Oxygen / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012440:Saccharomyces / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis; D014199:Trehalose / Q000032:analysis
chemical_list: D003470:Culture Media; D004952:Esters; D005231:Fatty Acids, Unsaturated; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D006003:Glycogen; D014199:Trehalose; C079238:alcohol O-acetyltransferase; D010100:Oxygen