Protective effect of ferulic acid ethyl ester against oxidative stress mediated by UVB irradiation in human epidermal melanocytes.
UV solar radiation is the major environmental risk factor for malignant melanoma. A great effort is currently posed on the search of new compounds able to prevent or reduce UV-mediated cell damage. Ferulic acid is a natural compound recently included in the formulation of solar protecting dermatological products. The purpose of the present work was to assess whether its ethyl ester derivative, FAEE, could protect skin melanocytes from UV-induced oxidative stress and cell damage. Experiments on human melanocytes irradiated with UVB showed that FAEE treatment reduced the generation of ROS, with a net decrease of protein oxidation. FAEE treatment was accompanied by an induction of HSP70 and heme oxygenase, by a marked suppression of PARP activation and a significant suppression of apoptosis. Moreover FAEE prevented iNOS induction, thus suppressing the secondary generation of NO-derived oxidizing agents. FAEE may represent a potentially effective pharmacological approach to reduce UV radiation-induced skin damage.
Di Domenico
F
F
Department of Biochemical Sciences, University of Rome La Sapienza, P.le Aldo Moro, 5-00185 Rome, Italy.
Perluigi
M
M
Foppoli
C
C
Blarzino
C
C
Coccia
R
R
De Marco
F
F
Butterfield
D A
DA
Cini
C
C
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002109:Caffeic Acids / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006801:Humans; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D013473:Sunscreening Agents / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D002109:Caffeic Acids; D050884:HSP72 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; C099085:ethyl ferulate; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D011065:Poly(ADP-ribose) Polymerases