title: NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
abstract: In this study, cancer cells were isolated from tumor specimens of nine glioblastoma patients. Glioblastoma cells, cultured under suitable culture conditions, displayed markers typical of neural stem cells, were capable of partial multilineage differentiation in vitro, and gave origin to infiltrating tumors when orthotopically injected in NOD/SCID mice. These cells, although resistant to freshly isolated NK cells, were highly susceptible to lysis mediated by both allogeneic and autologous IL-2 (or IL-15)-activated NK cells. Indeed, all stem cell-cultured glioblastoma cells analyzed did not express protective amounts of HLA class I molecules, while expressing various ligands of activating NK receptors that triggered optimal NK cell cytotoxicity. Importantly, glioblastoma stem cells expressed high levels of PVR and Nectin-2, the ligands of DNAM-1-activating NK receptor.
authors.affiliation: Dipartimento di Medicina Sperimentale, Università degli Studi di Genova, Genova, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Castriconi
authors.forename: Antonio
authors.initials: A
authors.lastname: Daga
authors.forename: Alessandra
authors.initials: A
authors.lastname: Dondero
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Zona
authors.forename: Pietro Luigi
authors.initials: PL
authors.lastname: Poliani
authors.forename: Alice
authors.initials: A
authors.lastname: Melotti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Griffero
authors.forename: Daniela
authors.initials: D
authors.lastname: Marubbi
authors.forename: Renato
authors.initials: R
authors.lastname: Spaziante
authors.forename: Francesca
authors.initials: F
authors.lastname: Bellora
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moretta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Moretta
authors.forename: Giorgio
authors.initials: G
authors.lastname: Corte
authors.forename: Cristina
authors.initials: C
authors.lastname: Bottino
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D005909:Glioblastoma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D008024:Ligands; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology / Q000637:transplantation; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D055607:Receptors, Natural Killer Cell / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D008024:Ligands; D055607:Receptors, Natural Killer Cell