Significance of transmission electron microscopy in subtyping of monocytic leukemia.
The objective of this paper is to produce an ultrastructural classification of acute monocytic leukemia (AML-M5) in relation to clinical behaviors. The ultrastructural characteristics of blasts of the monocytic series were analyzed in 72 M5 patients by transmission electron microscopy (TEM), in terms of their content of typical monoblasts, atypical monoblasts, atypical promonocytes, and typical promonocytes in bone-marrow aspirates. Four kinds of monocytic blasts were identified by cell size and shape, nuclear profile, nucleocytoplasmic ratio, heterochromatin content, nucleolus, granules, vesicles, and Golgi apparatus. Their characteristics of remission rate, cytochemistry, immunophenotype, and cytogenetics were also investigated. The data obtained permitted M5 patients to be divided into monoblast and promonocyte types. Monoblast-type patients expressed weaker monocytic enzymograms and specific antigen staining for CD14 and CD64, compared with promonocyte-type patients. Monoblast patients had higher CR than promonocyte patients. Therefore, TEM subclassification of patients differs from and improves upon the light microscopical criteria for distinguishing monoblasts and promonocytes and has clinical significance.
Ru
Yong-Xin
YX
Institute of Hematology & Blood Diseases Hospital, State Key Laboratory of Experimental Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. ruyongxin@126.com
Mi
Yin-Chang
YC
Liu
Jin-Hua
JH
Wang
Hui-Jun
HJ
Zhao
Shi-Xuan
SX
Cui
Wen
W
Li
Cheng-Wen
CW
Li
Qing-Hua
QH
Zhu
Xiao-Fan
XF
Xiao
Zhi-Jian
ZJ
Pang
Jian-Xiang
JX
Wang
Jian-Xiang
JX
D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007621:Karyotyping; D007948:Leukemia, Monocytic, Acute / Q000145:classification* / Q000175:diagnosis; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008875:Middle Aged; D055017:Monocyte-Macrophage Precursor Cells / Q000201:enzymology / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism; D055815:Young Adult
D009195:Peroxidase