Nanoscale live-cell imaging using hopping probe ion conductance microscopy.
We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.
Novak
Pavel
P
Imperial College London, UK.
Li
Chao
C
Shevchuk
Andrew I
AI
Stepanyan
Ruben
R
Caldwell
Matthew
M
Hughes
Simon
S
Smart
Trevor G
TG
Gorelik
Julia
J
Ostanin
Victor P
VP
Lab
Max J
MJ
Moss
Guy W J
GW
Frolenkov
Gregory I
GI
Klenerman
David
D
Korchev
Yuri E
YE
D000818:Animals; D002478:Cells, Cultured / Q000648:ultrastructure*; D004553:Electric Conductivity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D007477:Ions; D020527:Microscopy, Scanning Probe / Q000295:instrumentation* / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007477:Ions