Construction of cDNA libraries: focus on protists and fungi.
Sequencing of cDNA libraries is an efficient and inexpensive approach to analyze the protein-coding portion of a genome. It is frequently used for surveying the genomes of poorly studied eukaryotes, and is particularly useful for species that are not easily amenable to genome sequencing, because they are nonaxenic and/or difficult to cultivate. In this chapter, we describe protocols that have been applied successfully to construct and normalize a variety of cDNA libraries from many different species of free-living protists and fungi, and that require only small quantities of cell material.
Rodríguez-Ezpeleta
Naiara
N
Robert-Cedergren Centre for Bioinformatics and Genomics, Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.
Teijeiro
Shona
S
Forget
Lise
L
Burger
Gertraud
G
Lang
B Franz
BF
D018076:DNA, Complementary / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005658:Fungi / Q000235:genetics*; D015723:Gene Library; D005821:Genetic Techniques; D023281:Genomics; D012313:RNA / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000235:genetics
D018076:DNA, Complementary; D012313:RNA; D012194:RNA-Directed DNA Polymerase