The stability of biapenem and structural identification of impurities in aqueous solution.
The stability of biapenem in aqueous solution was investigated. Forced degradation of biapenem was carried out under different concentrations, pH values and temperatures. The degradation products were determined by reverse-phase HPLC and identified by LC-MS/MS. One dimeric impurity was obtained by reverse-phase preparative HPLC and characterized by LC-MS/MS and NMR. A possible degradation mechanism has been presented.
Xia
Ming
M
Department of Pharmacy, Jiangsu Simcere Pharmaceutical R&D Co Ltd, Nanjing, PR China. xiaming1217@163.com
Hang
Tai-Jun
TJ
Zhang
Fei
F
Li
Xiao-Min
XM
Xu
Xiang-Yang
XY
D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012996:Solutions; D013696:Temperature; D013845:Thienamycins / Q000032:analysis*; D014867:Water
D000890:Anti-Infective Agents; D012996:Solutions; D013845:Thienamycins; D014867:Water; C065257:biapenem