Cyclosporin A inhibits CD11a/CD18 adhesion molecules due to inhibition of TNFalpha and IL-1 beta levels in the mouse model of pleurisy induced by carrageenan.
The mouse model of pleurisy induced by carrageenan is characterized by a significant enhancement of cell migration due to neutrophils 4 h after pleurisy induction. Forty-eight hours after pleurisy induction, a significant increase in cell migration due to mononuclear cells occurs. Recently, studies in our laboratory have demonstrated that cyclosporine A (CsA) inhibits leukocyte migration in the pleural cavity and lungs in the mouse model of pleurisy induced by carrageenan. In the present work we evaluated whether CsA was able to downregulate CD11a/CD18 adhesion molecule in the lungs, as well as TNFalpha and IL-1 beta levels in the fluid leakage of the pleural cavity in this model. Our results showed that CsA significantly decreased CD11a/CD18 in the lungs, as well as TNFalpha and IL-1 beta levels in the fluid leakage of the pleural cavity 4 h and 48 h after pleurisy induction. It is our hypothesis that the inhibitory effect elicited by CsA upon these adhesion molecules may be also be attributed to the downregulation of TNFalpha and IL-1 beta cytokines.
Dalmarco
Eduardo Monguilhott
EM
Department of Pharmaceutical Sciences, Regional University of Blumenau, Itoupava Seca, Blumenau, Santa Catarina, Brazil.
Medeiros
Yara Santos
YS
Fröde
Tânia Silvia
TS
D000818:Animals; D040881:CD11a Antigen / Q000378:metabolism*; D018821:CD18 Antigens / Q000378:metabolism*; D002351:Carrageenan / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000378:metabolism*; D051379:Mice; D010998:Pleurisy / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D040881:CD11a Antigen; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016572:Cyclosporine; D002351:Carrageenan