Determination of impurities in oxidation hair dyes as raw materials by liquid chromatography (HPLC).
Synopsis Oxidation hair dyes as raw materials may present toxic impurities from synthesis or degradation pathways, which are responsible for allergic phenomena. In the present study selective and sensitive HPLC methods are proposed for the determination of Bandrowski base, 4-chloroaniline, 1,2-phenylenediamine and 4,4'-diaminoazobenzene in 1,4-phenylenediamine as well as 2,4-diaminoanisole in N-hydroxyethyl-4-methoxy-1,3-phenylenediamine. The proposed methods, based on reversed phase mode separation using 1,8-diaminooctane as amine modifier and photodiode array detection, proved to be suitable for the purity assessment of these widely used hair dyes; in particular the cited potential impurities were determined at ppm level.
Andrisano
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Department of Pharmaceutical Sciences, Via Belmeloro 6, 40126 Bologna, Italy.
Cavrini
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Summer
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Passuti
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