Potentiation of angiogenesis and regeneration by G-CSF after sciatic nerve crush injury.
Granulocyte colony-stimulating factor (G-CSF) demonstrates neuroprotective effects through different mechanisms, including mobilization of bone marrow cells. However, the influence of G-CSF-mediated mobilization of bone marrow-derived cells on injured sciatic nerves remains to be elucidated. The administration of G-CSF promoted a short-term functional recovery 7 days after crush injury in sciatic nerves. A double-immunofluorescence study using green fluorescent protein-chimeric mice revealed that bone marrow-derived CD34+ cells were predominantly mobilized and migrated into injured nerves after G-CSF treatment. G-CSF-mediated beneficial effects against sciatic nerve injury were associated with increased CD34+ cell deposition, vascular endothelial growth factor (VEGF) expression, and vascularization/angiogenesis as well as decreased CD68+ cell accumulation. However, cell differentiation and VEGF expression were not demonstrated in deposited cells. The results suggest that the promotion of short-term functional recovery in sciatic nerve crush injury by G-CSF involves a paracrine modulatory effect and a bone marrow-derived CD34+ cell mobilizing effect.
Pan
Hung-Chuan
HC
Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 407, Taiwan.
Wu
Hsi-Tien
HT
Cheng
Fu-Chou
FC
Chen
Cheng-Hsu
CH
Sheu
Meei-Ling
ML
Chen
Chun-Jung
CJ
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009416:Nerve Regeneration / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D012584:Sciatic Nerve / Q000187:drug effects* / Q000293:injuries* / Q000502:physiology
D018952:Antigens, CD34; D018696:Neuroprotective Agents; D016179:Granulocyte Colony-Stimulating Factor; D049452:Green Fluorescent Proteins