Unraveling inner ear induction by gene manipulation using Pax2-Cre BAC transgenic mice.
One of the biggest drawbacks of conventional mouse knockout techniques in the study of the inner ear is that loss of a gene of interest may cause embryonic lethality before the inner ear develops. Thus, there is a need for an inner ear-specific gene manipulation system for loss- and gain-of-function analysis in the mouse inner ear. We generated a Pax2-Cre BAC transgenic line in which Cre recombinase expression recapitulates Pax2 expression in the presumptive otic ectoderm. Here, we present a brief summary of a recent model of inner ear induction suggested by the results of inner ear-specific gene modification using Pax2-Cre mice.
Ohyama
Takahiro
T
Division of Cell Biology and Genetics, House Ear Institute, 2100 West Third Street, Los Angeles, CA 90057, USA. tohyama@hei.org
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000378:metabolism; D007758:Ear, Inner / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D019426:Integrases / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D051762:PAX2 Transcription Factor / Q000235:genetics* / Q000378:metabolism
D051762:PAX2 Transcription Factor; C045073:Cre recombinase; D019426:Integrases