Dual response surface-optimized process for feruloylated diacylglycerols by selective lipase-catalyzed transesterification in solvent free system.
Feruloylated diacylglycerol (FDAG) was synthesized using a selective lipase-catalyzed the transesterification between ethyl ferulate and triolein. To optimize the reaction conversion and purity of FDAG, dual response surface was applied to determine the effects of five-level-five-factors and their reciprocal interactions on product synthesis. A total of 32 individual experiments were performed to study reaction temperature, reaction time, substrate molar ratio, enzyme loading, and water activity. The highest reaction conversion and selectivity towards FDAG were 73.9% and 92.3%, respectively, at 55 degrees C, reaction time 5.3 day, enzyme loading 30.4 mg/ml, water activity 0.08, and a substrate molar ratio of 3.7. Moreover, predicted values showed good validation with the experimental values when experiments corresponding to selected points on the contour plots were carried out.
Zheng
Yan
Y
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai 201620, PR China.
Wu
Xiao-Mei
XM
Branford-White
Christopher
C
Quan
Jing
J
Zhu
Li-Min
LM
D000465:Algorithms; D002109:Caffeic Acids / Q000737:chemistry*; D002384:Catalysis; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004075:Diglycerides / Q000138:chemical synthesis*; D004951:Esterification; D008049:Lipase / Q000737:chemistry*; D011786:Quality Control; D012997:Solvents / Q000737:chemistry; D014304:Triolein / Q000737:chemistry*
D002109:Caffeic Acids; D004075:Diglycerides; D012997:Solvents; D014304:Triolein; C099085:ethyl ferulate; D008049:Lipase