Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?
OBJECTIVE
It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease.


METHODS
Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections.


RESULTS
Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn's disease, associated with emerging positivity of CD14(+) cells.


CONCLUSIONS
Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.
Frol'ová
Lenka
L
Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Videnska 1083, Prague 4 142 20, Czech Republic.
Smetana
Karel
K
Borovská
Dana
D
Kitanovicová
Andrea
A
Klimesová
Klára
K
Janatková
Ivana
I
Malícková
Karin
K
Lukás
Milan
M
Drastich
Pavel
P
Benes
Zdenek
Z
Tucková
Ludmila
L
Manning
Joachim C
JC
André
Sabine
S
Gabius
Hans-Joachim
HJ
Tlaskalová-Hogenová
Helena
H
D000818:Animals; D015415:Biomarkers; D015153:Blotting, Western; D003092:Colitis / Q000139:chemically induced; D003093:Colitis, Ulcerative / Q000097:blood; D003106:Colon / Q000378:metabolism; D003424:Crohn Disease / Q000097:blood; D016264:Dextran Sulfate; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D037502:Galectin 3 / Q000097:blood*; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000097:blood* / Q000139:chemically induced / Q000175:diagnosis; D037102:Lectins / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
D015415:Biomarkers; D005456:Fluorescent Dyes; D037502:Galectin 3; D037102:Lectins; D018950:Lipopolysaccharide Receptors; D016264:Dextran Sulfate; D016650:Fluorescein-5-isothiocyanate