Chemokine and chemokine receptor gene expression in the mesenteric adipose tissue of KKAy mice.
OBJECTIVE
To investigate chemokines and their receptors gene expression in the intra-abdominal adipose tissue of diabetic/obese mice.


METHODS
KKAy mice were fed either by a high-fat diet (HFD) or a low-fat diet (LFD) and obese characteristics were analyzed. Various adipose tissues were isolated from HFD-fed obese KKAy mice and from obese controls. We carried out RT-PCR, GeneChip microarray, and real-time PCR analyses on samples derived from the adipose tissues.


RESULTS
The HFD-feded obese KKAy mice had the physiological characteristics of obese animal and had increased levels of the transcripts of several chemokine and chemokine receptor genes, such as CCL5, CCL19, CCL25, CXCL10, CXCL13, CCR6, and CCR7, in their intra-abdominal adipose tissue. The strong expression of CCR6 and CCR7 was verified by microarray and quantitative real-time PCR analysis. The HFD increased CCR6 and CCR7 expression only in mesenteric (ME) adipose tissue, not in subcutaneous (SC) adipose tissue.


CONCLUSIONS
Since the enhanced expression of such molecules is likely to contribute to the inflammation in chronic inflammatory disease, our data suggest that the increased levels of CCR6 and CCR7 are involved in the inflammation response in the intra-abdominal adipose tissue of the obese/diabetic mice.
Lee
Han-Sam
HS
Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan, Nam-Ku Moogu-Dong San29, Ulsan 680-749, Republic of Korea.
Park
Jin-Hee
JH
Kang
Ji-Hye
JH
Kawada
Teruo
T
Yu
Rina
R
Han
In-Seob
IS
D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D018752:Diet, Fat-Restricted; D004041:Dietary Fats; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008297:Male; D008643:Mesentery / Q000033:anatomy & histology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009765:Obesity / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*
D001786:Blood Glucose; D018925:Chemokines; D004041:Dietary Fats; D019707:Receptors, Chemokine