Two-dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus.
The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immunosuppressed patients. We established a 2-D reference map for A. fumigatus. Using MALDI-TOF-MS/MS, we identified 381 spots representing 334 proteins. Proteins involved in cellular metabolism, protein synthesis, transport processes and cell cycle were most abundant. Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified.
Vödisch
Martin
M
Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knoell-Institute (HKI), Jena, Germany.
Albrecht
Daniela
D
Lessing
Franziska
F
Schmidt
André D
AD
Winkler
Robert
R
Guthke
Reinhard
R
Brakhage
Axel A
AA
Kniemeyer
Olaf
O
D001232:Aspergillus fumigatus / Q000166:cytology / Q000378:metabolism*; D002453:Cell Cycle; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D014176:Protein Biosynthesis; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D005656:Fungal Proteins; D020543:Proteome