A novel liquid multi-phytonutrient supplement demonstrates DNA-protective effects.
This study explored the DNA protective (anti-mutagenic) effects of an oral, liquid, multi-phytonutrient dietary supplement containing a proprietary blend of fruits, vegetables and aloe vera concentrated components in addition to a proprietary catechin complex from green tea (VIBE Cardiac & Life, Eniva Nutraceuticals, Anoka, MN; herein described as "VIBE"). This study tested the hypothesis that VIBE would reduce DNA damage in skin cells exposed to UVR. Human epidermal cells, from the cell line A431NS, were treated with 0% (control), 0.125%, 0.5%, 1% and 2% VIBE, and then exposed to 240 J/m(2) UVR. The amount of DNA damage was assessed using the COMET assay. At each concentration tested, a significantly smaller amount of DNA damage was measured by the COMET assay for the VIBE treated cells compared to the control cells exposed to UVR without VIBE. The dose response curves showed a maximal response at 0.5% VIBE with a threefold reduction in COMET tail density compared to the control samples without VIBE (p < 0.001). Additional research is warranted in human clinical trials to further explore the results of this study which demonstrated the DNA protective and anti-mutagenic effects of VIBE for human skin cells exposed to UVR-induced DNA damage.
Baechler
Benjamin J
BJ
Eniva Nutraceuticals, Blaine, MN 55434, USA. baec0004@umn.edu
Nita
Florina
F
Jones
Lon
L
Frestedt
Joy L
JL
D016587:Antimutagenic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D002392:Catechin; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D019587:Dietary Supplements; D004307:Dose-Response Relationship, Radiation; D004338:Drug Combinations; D005638:Fruit; D006801:Humans; D019684:Magnoliopsida; D009381:Neoplasms, Radiation-Induced / Q000517:prevention & control; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011837:Radiation-Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D012878:Skin Neoplasms / Q000517:prevention & control; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014675:Vegetables
D016587:Antimutagenic Agents; D000975:Antioxidants; D004338:Drug Combinations; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D002392:Catechin