title: Piscidin 4, a novel member of the piscidin family of antimicrobial peptides.
abstract: Piscidins are linear, amphipathic, antimicrobial peptides (AMPs) with broad, potent, activity spectrum. Piscidins and other members of the piscidin family appear to comprise the most common group of AMPs in teleost fish. All piscidins and related members of the piscidin family described to date are 18-26 amino acids long.We report here the isolation of a novel 5329.25 Da, 44-residue (FFRHLFRGAKAIFRGARQGXRAHKVVSRYRNRDVPETDNNQEEP) antimicrobial peptide from hybrid striped bass (Morone chrysops female x M. saxatilis male).We have named this peptide "piscidin 4" since it has considerable (to >65%) N-terminal sequence homology to piscidins 1-3 and this distinctive, 10 to 11-residue, N-terminus is characteristic of piscidins. The native peptide has a modified amino acid at position 20 that, based upon mass spectrometry data, is probably a hydroxylated tryptophan. Synthetic piscidin 4 (with an unmodified tryptophan at position 20) has similar antibacterial activity to that of the native peptide. Piscidin 4 demonstrates potent, broad-spectrum, antibacterial activity against a number of fish and human pathogens, including multi-drug resistant bacteria. Its potent antimicrobial activity suggests that piscidin 4 plays a significant role in the innate defense system of hybrid striped bass.
authors.affiliation: Department of Clinical Sciences, North Carolina State University, Raleigh, 27606, USA. ed_noga@ncsu.edu
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Noga
authors.forename: Uma
authors.initials: U
authors.lastname: Silphaduang
authors.forename: Nam Gyu
authors.initials: NG
authors.lastname: Park
authors.forename: Jung Kil
authors.initials: JK
authors.lastname: Seo
authors.forename: Jeana
authors.initials: J
authors.lastname: Stephenson
authors.forename: Shannon
authors.initials: S
authors.lastname: Kozlowicz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001492:Bass; D004926:Escherichia coli / Q000187:drug effects; D017045:Lactococcus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010776:Photobacterium / Q000187:drug effects; D011513:Proteus mirabilis / Q000187:drug effects; D016415:Sequence Alignment; D012763:Shigella flexneri / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
chemical_list: D023181:Antimicrobial Cationic Peptides; C541375:piscidin 4, striped bass