title: POSTMan (POST-translational modification analysis), a software application for PTM discovery.
abstract: Post-translationally modified peptides present in low concentrations are often not selected for CID, resulting in no sequence information for these peptides. We have developed a software POSTMan (POST-translational Modification analysis) allowing post-translationally modified peptides to be targeted for fragmentation. The software aligns LC-MS runs (MS(1) data) between individual runs or within a single run and isolates pairs of peptides which differ by a user defined mass difference (post-translationally modified peptides). The method was validated for acetylated peptides and allowed an assessment of even the basal protein phosphorylation of phenylalanine hydroxylase (PHA) in intact cells.
authors.affiliation: PROBE Proteomic Platform, Department of Biomedicine, University of Bergen, Bergen, Norway.
authors.forename: Magnus Ø
authors.initials: MØ
authors.lastname: Arntzen
authors.forename: Christoffer Leif
authors.initials: CL
authors.lastname: Osland
authors.forename: Christopher Rasch-Olsen
authors.initials: CR
authors.lastname: Raa
authors.forename: Reidun
authors.initials: R
authors.lastname: Kopperud
authors.forename: Stein-Ove
authors.initials: SO
authors.lastname: Døskeland
authors.forename: Aurélia E
authors.initials: AE
authors.lastname: Lewis
authors.forename: Clive S
authors.initials: CS
authors.lastname: D'Santos
mesh_terms: D000107:Acetylation; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D010651:Phenylalanine Hydroxylase / Q000032:analysis / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012984:Software
chemical_list: D010455:Peptides; D010651:Phenylalanine Hydroxylase