Generation of full-length cDNA libraries: focus on plants.
Full-length cDNAs are essential for the correct annotation of transcriptional units and gene products from genomic sequence data and for functional analysis of the genes. Full-length cDNA libraries are very important resources for isolation of the full-length cDNAs. The biotinylated cap trapper method using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter.
Seki
Motoaki
M
Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, Yokohama, Japan.
Kamiya
Asako
A
Carninci
Piero
P
Hayashizaki
Yoshihide
Y
Shinozaki
Kazuo
K
D017360:Arabidopsis / Q000235:genetics; D019921:Biotinylation; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D018744:DNA, Plant; D015723:Gene Library; D017343:Genes, Plant; D005821:Genetic Techniques; D015698:Genomic Library; D008956:Models, Chemical; D008957:Models, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D014199:Trehalose / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D014199:Trehalose