title: The thyroid hormone receptor (TR) beta-selective agonist GC-1 inhibits proliferation but induces differentiation and TR beta mRNA expression in mouse and rat osteoblast-like cells.
abstract: Previous studies showed anabolic effects of GC-1, a triiodothyronine (T3) analogue that is selective for both binding and activation functions of thyroid hormone receptor (TR) beta1 over TRalpha1, on bone tissue in vivo. The aim of this study was to investigate the responsiveness of rat (ROS17/2.8) and mouse (MC3T3-E1) osteoblast-like cells to GC-1. As expected, T3 inhibited cellular proliferation and stimulated mRNA expression of osteocalcin or alkaline phosphatase in both cell lineages. Whereas equimolar doses of T3 and GC-1 equally affected these parameters in ROS17/2.8 cells, the effects of GC-1 were more modest compared to those of T3 in MC3T3-E1 cells. Interestingly, we showed that there is higher expression of TRalpha1 than TRbeta1 mRNA in rat (approximately 20-90%) and mouse (approximately 90-98%) cell lineages and that this difference is even higher in mouse cells, which highlights the importance of TRalpha1 to bone physiology and may partially explain the modest effects of GC-1 in comparison with T3 in MC3T3-E1 cells. Nevertheless, we showed that TRbeta1 mRNA expression increases (approximately 2.8- to 4.3-fold) as osteoblastic cells undergo maturation, suggesting a key role of TRbeta1 in mediating T3 effects in the bone forming cells, especially in mature osteoblasts. It is noteworthy that T3 and GC-1 induced TRbeta1 mRNA expression to a similar extent in both cell lineages (approximately 2- to 4-fold), indicating that both ligands may modulate the responsiveness of osteoblasts to T3. Taken together, these data show that TRbeta selective T3 analogues have the potential to directly induce the differentiation and activity of osteoblasts.
authors.affiliation: Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes 2415, Sao Paulo, SP, 05508-000, Brazil.
authors.forename: Eduardo H
authors.initials: EH
authors.lastname: Beber
authors.forename: Luciane P
authors.initials: LP
authors.lastname: Capelo
authors.forename: Tatiana L
authors.initials: TL
authors.lastname: Fonseca
authors.forename: Cristiane C
authors.initials: CC
authors.lastname: Costa
authors.forename: Claudimara F
authors.initials: CF
authors.lastname: Lotfi
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Scanlan
authors.forename: Cecilia H A
authors.initials: CH
authors.lastname: Gouveia
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D015870:Gene Expression; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics; D051381:Rats; D037042:Thyroid Hormone Receptors beta / Q000819:agonists* / Q000235:genetics; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D000085:Acetates; C413355:GC 1 compound; D010636:Phenols; D012333:RNA, Messenger; D037042:Thyroid Hormone Receptors beta; D014284:Triiodothyronine; D015675:Osteocalcin; D000469:Alkaline Phosphatase