title: Human antibody repertoires.
abstract: Symplex is an antibody discovery technology that identifies fully human antigen-specific antibody repertoires directly from plasma cells. The technology utilizes reverse transcription and overlap extension polymerase chain reaction performed on single-cell-sorted plasma cells, whereby the heavy- and light-chain cognate pairing of the antibodies is maintained. The isolated antibodies from a plasma cell donor reflect the diversity, affinity, and selectivity of the donor antibody repertoire, making the technology an ideal tool for identifying drug leads and studying the development of human antibody repertoires.
authors.affiliation: Symphogen, Lyngby, Denmark.
authors.forename: Per-Johan
authors.initials: PJ
authors.lastname: Meijer
authors.forename: Lars S
authors.initials: LS
authors.lastname: Nielsen
authors.forename: Johan
authors.initials: J
authors.lastname: Lantto
authors.forename: Allan
authors.initials: A
authors.lastname: Jensen
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007114:Immunization; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D000906:Antibodies; D007140:Immunoglobulin Fab Fragments