[Biochemical methods for the analysis of DNA-protein interactions].
Investigation of DNA-protein interactions is fundamental to understand the mechanism underlying a variety of life processes. In this article, various types of biochemical methods in DNA-protein interaction study in vivo and in vitro at the level of DNA, protein, and the complex, respectively were briefly reviewed. Traditional assays including Nitrocellulose filter-binding assay, Footprinting, EMSA, and Southwestern blotting were summarized. In addition, chromatin immunoprecipitation techniques including nChIP, xChIP, and ChIP-on-chip, which were widely used in epigenetics, were particularly introduced.
Zhang
Jin-Bi
JB
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. 2007105010@njau.edu.cn
Pan
Zeng-Xiang
ZX
Lin
Fei
F
Ma
Xue-Shan
XS
Liu
Hong-Lin
HL
D015139:Blotting, Southern; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D008957:Models, Genetic; D011485:Protein Binding; D023302:Protein Footprinting; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
D011506:Proteins; D004247:DNA