title: High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis.
abstract: The alkaloid ricinine, an insecticide for leaf-cutting ant (Atta sexdens rubropilosa), was obtained from Ricinus communis. A two-phase solvent system composed of CH(2)Cl(2)/EtOH/H(2)O (93:35:72, v/v/v) was used for high-speed counter-current chromatographic (HSCCC) isolation of ricinine in high yield and with over 96% purity, as determined by liquid and gas chromatography-mass spectrometry (LC-MS and GC-MS). Identification of ricinine was performed by comparison of (1)H NMR, (13)C NMR and LC-MS/MS data.
authors.affiliation: Universidade Federal de São Carlos, Departamento de Química, SP, Brazil.
authors.forename: Cristiane de Melo
authors.initials: Cde M
authors.lastname: Cazal
authors.forename: Jaqueline Raquel
authors.initials: JR
authors.lastname: Batalhão
authors.forename: Vanessa de Cássia
authors.initials: Vde C
authors.lastname: Domingues
authors.forename: Odair Corrêa
authors.initials: OC
authors.lastname: Bueno
authors.forename: Edson Rodrigues
authors.initials: ER
authors.lastname: Filho
authors.forename: Moacir R
authors.initials: MR
authors.lastname: Forim
authors.forename: Maria Fátima G Fernandes
authors.initials: MF
authors.lastname: da Silva
authors.forename: Paulo Cezar
authors.initials: PC
authors.lastname: Vieira
authors.forename: João Batista
authors.initials: JB
authors.lastname: Fernandes
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification*; D000818:Animals; D001000:Ants; D002853:Chromatography, Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007306:Insecticides / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011728:Pyridones / Q000302:isolation & purification*; D012278:Ricinus / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000470:Alkaloids; D007306:Insecticides; D010936:Plant Extracts; D011728:Pyridones; D012997:Solvents; C019550:ricinine