title: Beneficial effect of autologous endometrial cell coculture in patients with repeated implantation failure.
abstract: OBJECTIVE
  To confirm the beneficial effect of endometrial coculture in patients with repeated failures with assisted reproductive techniques (ART).
  
  
  METHODS
  Patients with previous failures were offered a repetition of ART in conjunction with autologous endometrial coculture.
  
  
  METHODS
  Private fertility center.
  
  
  METHODS
  Sixty-eight couples who had attempted 92 cycles of IVF or intracytoplasmic sperm injection without obtaining an evolutive pregnancy.
  
  
  METHODS
  Patients repeated one cycle of ART with concomitant endometrial coculture of their embryos.
  
  
  METHODS
  Comparative pregnancy and delivery rates in conventional ART cycles vs. cycles with autologous endometrial coculture.
  
  
  RESULTS
  In the previous 92 cycles (146 ETs, fresh plus frozen) only 8 pregnancies were initiated, and all ended in spontaneous abortion. Upon repeating 68 cycles (76 ETs) using coculture, 39 pregnancies were obtained, of which 19 resulted in live births, 10 are ongoing evolutive pregnancies, and 10 ended in spontaneous abortions.
  
  
  CONCLUSIONS
  These results confirm the usefulness of autologous endometrial coculture for the treatment of patients with repeated implantation failure.
authors.affiliation: Centro Médico Fertilab, Buenos Aires, Argentina.
authors.forename: Victoria
authors.initials: V
authors.lastname: Eyheremendy
authors.forename: Fernanda G E
authors.initials: FG
authors.lastname: Raffo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Papayannis
authors.forename: Julia
authors.initials: J
authors.lastname: Barnes
authors.forename: Cintia
authors.initials: C
authors.lastname: Granados
authors.forename: Jorge
authors.initials: J
authors.lastname: Blaquier
mesh_terms: D000328:Adult; D018920:Coculture Techniques; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic